LOCUS       KHUT01000001             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000001
VERSION     KHUT01000001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           87 g           52 t
ORIGIN      
        1 caccggcagc tcaagtggtg gccgttttta ttgggcctaa agcgttcgta gccggcttga
       61 taagtctctg gtgaaatctc acggcttaac cgtgagaatt gctggagata ctattaggct
      121 tgaggccggg agaggttagc ggtactcccg gggtaggggt gaaatcctat aatcccggga
      181 ggaccacctg tggcgaaggc ggctaactgg aacggacctg acggtgagta acgaaagcca
      241 ggggcgcgaa ccgg
//
LOCUS       KHUT01000002             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000002
VERSION     KHUT01000002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           88 g           50 t
ORIGIN      
        1 caccggcggc ccgagtggtg atcactatta ttgggtctaa agggtccgta gccggtttga
       61 tcagtcctct gggaaatctg acagctcaac tgttaggctt ccaggggata ctgtcagact
      121 tgggaccggg agaggtaaga ggtaccacag gggtaggagt gaaatcttgt aatccctgtg
      181 ggaccaccag tggcgaaggc gtcttaccag aacgggtccg acggtgaggg acgaaagctg
      241 ggggcacgaa ccgg
//
LOCUS       KHUT01000003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000003
VERSION     KHUT01000003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           86 g           51 t
ORIGIN      
        1 caccgacggc ccaagtggtg accactctta ttgggtctaa agcgtccgta gccggtttag
       61 taagtccttg tttaaatcct ctggcttaac cagaggactg gcagggatac tgctagactt
      121 gggaccggga gaggacaagg gtactccaag ggtagcggtg gaatgtgttg atccttggag
      181 gaccacctat ggcgaaggca cttgtctgga acgggtccga cggtgaggga cgaaagccag
      241 gggcgcgaac cgg
//
LOCUS       KHUT01000004             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000004
VERSION     KHUT01000004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           89 g           46 t
ORIGIN      
        1 taccggcagt ccgagtgatg gccgatctta ttgggcctaa agcgtccgta gctggctgaa
       61 caagtccgtt gggaaatctg tccgcttaac gggcaggcgt ccagcggaaa ctgttcagct
      121 tgggaccgga agacctgagg ggtacgtctg gggtaggagt gaaatcctgt aatcctggac
      181 ggaccgccgg tggcgaaagc gcctcaggag aacggatccg acagtgaggg acgaaagcta
      241 gggtctcgaa ccgg
//
LOCUS       KHUT01000005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000005
VERSION     KHUT01000005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           53 c           87 g           43 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 taagtccgcc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttccgg tggagcggtg aaatgcgtag agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000006
VERSION     KHUT01000006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           88 g           49 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtgggttgt
       61 gaagtcttct gttaaagatc agggcttaac cctgtttagg cagtggaaac tcataaccta
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccgac ggcgaaagca ctctgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000007             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000007
VERSION     KHUT01000007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           66 c           91 g           41 t
ORIGIN      
        1 taccggcagc acgagtgatg gccgctctta ttgggcctaa agcgtccgta gctggccgcg
       61 caagtccatc gggaaatccg cacgcttaac gtgcgggcgt ccggtggaaa ctgcgtggct
      121 tgggaccgga agatccagag ggtacgtccg gggtaggagt gaaatcctgt aatcctggac
      181 ggaccaccgg tggcgaaagc gctctggaaa gacggatccg acggtgaggg acgaaagctc
      241 gggtcacgaa ccgg
//
LOCUS       KHUT01000008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000008
VERSION     KHUT01000008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtctgtc gtgaaagccc cgggctcaac ctgggaattg cgatggagac tgcaaggctt
      121 gaatctggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaagattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000009
VERSION     KHUT01000009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           92 g           45 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcagtt
       61 caagtctgct gtcaaagacc ggggcttaac ttcggaaagg cagtggaaac tgaacagcta
      121 gagtatggta ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacatcggt ggcgaaggcg ctctgctgga ccataactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000010
VERSION     KHUT01000010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgcc gtgaaagtcc ggggctcaac tccggatctg cggtgggtac gggcagacta
      121 gagtgatgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg cattaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000011
VERSION     KHUT01000011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tgggggactt
      121 gagtgcagga gagaagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctttggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000012
VERSION     KHUT01000012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgcgg
       61 taagtcgggt gtgaaatctc ggagcttaac tccgaaactg cattcgatac tgccgtgctt
      121 gaggactgga gaggagactg gaatttacgg tgtagcggtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000013
VERSION     KHUT01000013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcgggtctt
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tgaagatctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000014
VERSION     KHUT01000014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           90 g           46 t
ORIGIN      
        1 tacgggggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggctaat
       61 caagtctgct gtcaaaggtt ggggcttaac cccaaaaagg cagtggaaac tgagaagcta
      121 gagtgcagta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga ctgcaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000015
VERSION     KHUT01000015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           99 g           43 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggctcgg
       61 aaagtcggtc gtgaaatgcc ggggctcaac cccgggactg cgtccgatac ttccgggctg
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU16 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000016
VERSION     KHUT01000016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU16"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggattgc
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tggcgacctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU17 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000017
VERSION     KHUT01000017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU17"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           58 c           84 g           45 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggcctta
       61 caagtcctcc gttaaatcca gctgcttaac agatgggctg cggaggatac tataagacta
      121 ggaggcagga gaggcaagcg gtactcagtg ggtaggggta aaatccgttg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga atgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU18 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000018
VERSION     KHUT01000018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU18"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtcggct gtgaaagtcc agggctcaac cctgggatgc cggtcgatac tgccggacta
      121 gagttcggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacacctat ggcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU19 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000019
VERSION     KHUT01000019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU19"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacgtaggtg acaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgat
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cattcgatac tgatcgactc
      121 gaaggcagga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU20 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000020
VERSION     KHUT01000020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU20"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctgtt
       61 caagtcgaac gtgaaaactc ggggctcaac tccgagcctg cgttcgatac tggatggcta
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU21 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000021
VERSION     KHUT01000021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU21"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           93 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttagt
       61 taagtctgtt gtgaaatccc cgggctcaac ctgggaatgg caatggatac tggctggcta
      121 gagtgtgtca gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccatctggg acaacactga cgctgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU22 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000022
VERSION     KHUT01000022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU22"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggccact
       61 caagtcgaat gtgaaacctc gaggcttaac ctcgagcctg cattcgatac tgggcggcta
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU23 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000023
VERSION     KHUT01000023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU23"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgct gtgaaaactg gaggctcaac ctccagcctg cagtgggtac gggcagacta
      121 gagtgcggta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gatctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU24 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000024
VERSION     KHUT01000024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU24"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggctagg
       61 taggtccgtt gtgaaaactc gaggctcaac ctcgagacgc cggtggaaac catctagcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU25 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000025
VERSION     KHUT01000025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU25"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcag
       61 aaagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac ttctgagctg
      121 gagttctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU26 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000026
VERSION     KHUT01000026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU26"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcactt gtgaaatctc cgggcttaac ccggagcggc caagtgatac tgtcgtgcta
      121 gagtgcggaa ggggctactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU27 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000027
VERSION     KHUT01000027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU27"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtctgt
       61 taagtcatgt gtgaaatccc tcggctcaac cggggaagga cgcatgaaac tgacaagcta
      121 gagtaccaaa gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccccctggt tggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU28 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000028
VERSION     KHUT01000028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU28"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtacgca ggcggctttg
       61 caagacaggt gtgaaatccc cgggcctaac ctgggaactg catttgtgac tgcatggcta
      121 gagtttgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg atgtgactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU29 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000029
VERSION     KHUT01000029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU29"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           89 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctatg
       61 taagtctgct gttaaagaat ctggctcaac cagataaagg cagtggaaac tacatggcta
      121 gagtgcgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU30 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000030
VERSION     KHUT01000030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU30"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgccg
       61 taagtcgggt gtgaaatttc ggagcttaac tccgaaactg cattcgatac tgcggtgctt
      121 gaggactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag agatcatgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU31 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000031
VERSION     KHUT01000031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU31"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           60 c           83 g           45 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtcttg
       61 caagtcttcc gttaaatcca cctgcttaac agatgggctg cggaagatac tacaagacta
      121 ggaggcggga gaggcaagcg gtactcagtg ggtaggggta aaatcctctg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000032             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU32 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000032
VERSION     KHUT01000032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU32"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           99 g           42 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccacc
       61 gcaagtcgac tgtgaaatct ccgggcttaa cccggaaagg tcagccgata ctgcggggct
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU33 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000033
VERSION     KHUT01000033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU33"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggaggactt
      121 gagtgcagaa gaggaaagtg gaattccatg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU34 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000034
VERSION     KHUT01000034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU34"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          101 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcctgt
       61 tgcgtccgct gtgaaagccc ggggcttaac cccgggtctg cagtggatac gggcaggcta
      121 gagtccggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU35 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000035
VERSION     KHUT01000035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU35"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcgg
       61 taagtcactt gtgaaatctc tgagcttaac tcagaacggc caagtgatac tgcagtgcta
      121 gagtgtggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccaacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU36 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000036
VERSION     KHUT01000036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU36"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           98 g           49 t
ORIGIN      
        1 tacgtagggc gcgagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgcc gtgaaagtcc ggggcttaac cccggatctg cggtgggtac gggcagactg
      121 gagtgcagta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU37 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000037
VERSION     KHUT01000037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU37"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           87 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttg
       61 taagtcagtg gtgaaatatg gcagctcaac tgtcagggtg ccattgatac tgcggagctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactggg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU38 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000038
VERSION     KHUT01000038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU38"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           80 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgatgagctt
      121 gaatacacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactatg gtgtcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU39 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000039
VERSION     KHUT01000039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU39"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          103 g           45 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtttga
       61 taagggtgtg gtgaaagtcc ggggctcaac cccggatctg ccgtgccgac tgtcaaactc
      121 gaggactgta gaggcagacg gaattccggg tgtagcggtg gaatgcgtag agatccggag
      181 gaagaccggt ggcgaaggcg gtctgctggg cagtttctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU40 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000040
VERSION     KHUT01000040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU40"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagcg gtgaaatccc agggctcaac cctggaactg ccgttgatac tgcagggctt
      121 gagttcggtt aaggcgggcg gaactggtgg tgtagcggtg aaatgcatag ataccaccaa
      181 gaaccccgat tgcgtaggca gctcgctgag ccgaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU41 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000041
VERSION     KHUT01000041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU41"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           61 c           86 g           44 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtactg
       61 caagtcttcc gttaaatcca cctgcttaac agttgggctg cgggggatac tacagcgcta
      121 ggaggcggga gaggcaagcg gtactcaatg ggtaggggta aaatccgctg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU42 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000042
VERSION     KHUT01000042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU42"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggctgttcgg
       61 taagtctgcc gtgaaaacct gaggctcaac ctcgggcgtg cggtggatac tgccgggcta
      121 gaggacggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccgttcctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000043             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU43 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000043
VERSION     KHUT01000043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU43"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccagc
       61 gcaagtcagt tgtgaaatct ccgagcttaa ctcggaaagg tcaactgata ctgcgcggct
      121 agagtgcgga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000044             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU44 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000044
VERSION     KHUT01000044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU44"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatca ttgggcgtaa agagctcgta ggcggttcag
       61 caagtcggct gtgaaagtcc aaggcttaac cttggaaatg cagtcgatac tgctgtgact
      121 agagtctggt agaggagaat ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gcctcgactg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU45 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000045
VERSION     KHUT01000045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU45"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatcgt
       61 taagtcagag gtgaaatccc agggctcaac cctggaactg cctttgatac tggcgatctt
      121 gagtatgaga gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggc tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU46 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000046
VERSION     KHUT01000046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU46"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaatgg cattggatac tggcgagcta
      121 gagtgcggta gagggtagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg actacctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000047             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU47 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000047
VERSION     KHUT01000047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU47"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           45 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcgggt gtgaaaccct caggctcaac ctggggacgc cactcgatac tgctgtggct
      121 agagtccagt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcactctgg gctggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU48 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000048
VERSION     KHUT01000048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU48"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           76 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcaag
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatttgtctt
      121 gaatactgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU49 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000049
VERSION     KHUT01000049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU49"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccaac
       61 taagtcagac gtgaaatccc caggcttaac ctgggaactg cgtctgatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgagattga cactgatgag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU50 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000050
VERSION     KHUT01000050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU50"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           99 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 tgtgtcggtg gtgaaagccc cgggcttaac ctgggaatgg ccatggaaac tggcgaactg
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ctagcactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU51 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000051
VERSION     KHUT01000051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU51"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtctgct gtgaaaactc gaggctcaac ctcgagatgt cggcggaaac catctggcta
      121 gagtccggaa gaggaaagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctttctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU52 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000052
VERSION     KHUT01000052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU52"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           98 g           43 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaaaccc gcggctcaac cgcgggcctg cagccgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU53 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000053
VERSION     KHUT01000053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU53"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcgaac gtgaaatccc tgggctcaac ccaggaactg cgtccgatac tggaaggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU54 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000054
VERSION     KHUT01000054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU54"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcagagctt
      121 gagtgccgga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000055             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU55 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000055
VERSION     KHUT01000055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU55"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           49 t
ORIGIN      
        1 tacgtagggt cctagcgtta tccggaatca ttgggcgtaa agagctcgta ggcggttcag
       61 caagtcggct gtgaaagtcc gaggctcaac ctcggaattg cagtcgatac tgctgtgact
      121 cgagtccggt agaggagaat ggaattctcg gtgtagcggt gaaatgcgca gatatcgaga
      181 ggaacaccag tagcgaaggc ggttctctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU56 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000056
VERSION     KHUT01000056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU56"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           82 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcaggg gtgaaagacg gtagctcaac tatcgcagtg cccttgatac tgatgggctt
      121 gaatggacta gaggtaggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gcttactatg gtcttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000057             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU57 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000057
VERSION     KHUT01000057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU57"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcagac gtgaaatccc tcggcttaac cggggaactg cgtctgatac tggatggctt
      121 gagtttggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaacactga cgctgagtga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU58 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000058
VERSION     KHUT01000058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU58"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          100 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcggat gtgaaaaccc ggggctcaac cccgggcctg cattcgatac tgtcgggctt
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01000059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU59 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000059
VERSION     KHUT01000059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU59"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggcttct
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tgggaagctc
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU60 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000060
VERSION     KHUT01000060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU60"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagtcgggt gtgaaatccc caggcttaac ctgggaactg cattcgagac tgcattgcta
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU61 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000061
VERSION     KHUT01000061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU61"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           57 c           85 g           47 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggttcta
       61 caagtcttcc gttaaatcca cctgcttaac agatgggctg cggaggatac tatggagcta
      121 ggaggcggga gaggcaagcg gtacttagtg ggtaggggta aaatccgttg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcactcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU62 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000062
VERSION     KHUT01000062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU62"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaagccc ggggctcaac cccgggtctg cagtgggtac gggcagacta
      121 gagtgcagta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU63 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000063
VERSION     KHUT01000063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU63"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaaacct caggctcaac ctggggcttg cagtgggtac gggcagacta
      121 gagtgcggta ggggtgactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtcactggg ccgcaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU64 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000064
VERSION     KHUT01000064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU64"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          100 g           41 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggccgtg
       61 caagtcggtt gtgaaatccc ggggctcaac cccgggactg cgtccgatac tgcacggctc
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctgga cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU65 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000065
VERSION     KHUT01000065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU65"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcgg
       61 taagtcagct gtgaaatctc cgagcttaac tcggaacggc cagctgatac tgcagtgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU66 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000066
VERSION     KHUT01000066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU66"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           74 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttaa
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgttaaactt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU67 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000067
VERSION     KHUT01000067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU67"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          102 g           39 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctttg
       61 gccgtcggct gtgaaagccc ggggctcaac cccggaggtg cagccgagac cccgaagctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU68 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000068
VERSION     KHUT01000068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU68"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtctgct gtgaaaactc gaggctcaac ctcgagctgt cggtggaaac catctggcta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU69 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000069
VERSION     KHUT01000069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU69"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagttagag gtgaaagccc ggggctcaac tccggaattg cctttaagac tgcatcgcta
      121 gaattgtgga gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acttactgga cacatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU70 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000070
VERSION     KHUT01000070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU70"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c           98 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgct gtgaaatccc gaggctcaac ctcgggtctg cagtgggtac gggcagactg
      121 gagtgcggta ggggagaatg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gttctctggg ccgtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU71 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000071
VERSION     KHUT01000071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU71"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttat
       61 caagtcaggc gtgaaattcc cgggctcaac ctgggggctg cgcttgatac tgatgagctt
      121 gaatgcggga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctatctggc ccgtaattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU72 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000072
VERSION     KHUT01000072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU72"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           88 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctgtg
       61 taagtctgct gttaaagagt ctagctcaac tagataaaag cagtggaaac tacacggcta
      121 gagtacgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccagt ggcgaaggcg ctctgctagg ccgtaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU73 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000073
VERSION     KHUT01000073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU73"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           94 g           50 t
ORIGIN      
        1 tacggggggg gtaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcatac gtgaaatccc tcagcttaac tggggaactg cgtgtgatac tggatggctt
      121 ggatttggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaatattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU74 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000074
VERSION     KHUT01000074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU74"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           76 g           64 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattca ctgggtttaa agggtgcgta ggtggggaag
       61 taagtcagtg gtgaaatctc cgtgcttaac atggaaactg ccattgatac tatttttctt
      121 gaattttccg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctaactaca gggatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU75 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000075
VERSION     KHUT01000075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU75"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttgtt
       61 taagtctgct gtgaaagccc tgggctcaac ctgggaattg cagtggatac tgggcgacta
      121 gagtgtggta gaggatagtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaacatctgt ggcgaaggcg actatctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU76 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000076
VERSION     KHUT01000076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU76"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggccaga
       61 taagtccgct gtgaaatctc gaggctcaac ttcgagctgt cggtggaaac tgtctggcta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU77 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000077
VERSION     KHUT01000077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU77"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggtggcctca
       61 taagtccgct gtgaaagtca aaggctcaac ctttgaaagc cggtggatac tgtgaggcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU78 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000078
VERSION     KHUT01000078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU78"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttatt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgagac tgaatagcta
      121 gggtgcggta gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccagcaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU79 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000079
VERSION     KHUT01000079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU79"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           92 g           45 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcactt
       61 caagtctgtc gtcaaagact ggggcttaac cctggaaagg cggtggaaac tgaagcgcta
      121 gagtgtggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga ccacaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU80 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000080
VERSION     KHUT01000080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU80"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           86 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg ctgaaatatc ccagcttaac tgggagggtg gcattgatac tgcggggcta
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU81 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000081
VERSION     KHUT01000081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU81"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtcggct gtgaaatccc gaggctcaac ctcgggtctg cagtcgatac gagcaaacta
      121 gagtgttgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caacaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU82 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000082
VERSION     KHUT01000082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU82"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtccggt gtgaaaactc gaggcttaac ctcgagatgt catcggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaagca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU83 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000083
VERSION     KHUT01000083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU83"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccga
       61 taagtccgct gtgaaagtcc agggctcaac cctgggatgc cggtggatac tgtcgggctc
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gcactctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU84 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000084
VERSION     KHUT01000084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU84"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagccagat gtgaaagccc cgggcttaac ctgggaatgg catttgggac tgcaaggctt
      121 gagtgcggca gaggagactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaataccgat ggcgaaggca ggtctctggg ctgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU85 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000085
VERSION     KHUT01000085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU85"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           87 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggctatt
       61 caagtctgct gttaaagcac ggggcttaac cccgaaaagg cagtggaaac tgaaaagcta
      121 gagtgcagta ggggtaaagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctttactgga ctgcaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU86 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000086
VERSION     KHUT01000086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU86"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtctga
       61 ttagtctgat gtgaaagccc tgggctcaac ccaggaagtg cattggatac tgtcagactt
      121 gaatacggga gagggtagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccgatattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU87 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000087
VERSION     KHUT01000087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU87"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 tgcgtccgct gtgaaaactc ggggcttaac cccgagcctg cagtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000088             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU88 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000088
VERSION     KHUT01000088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU88"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcgtg
       61 acaagtcact tgtgaaatct ccgggcttaa ctcggaacgg tcaagtgata ctgtcggact
      121 agagtacaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgatactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU89 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000089
VERSION     KHUT01000089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU89"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtaag
       61 taagtccgtg gtgaaatctc tgagcttaac tcagaaactg ccgtggatac tatttgcctt
      121 gaatattgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca caatcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU90 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000090
VERSION     KHUT01000090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU90"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtactt
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaaccg cgcttgagac tggagaacta
      121 gagttcggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgat ggcgaaggca gccatctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU91 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000091
VERSION     KHUT01000091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU91"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggttact
       61 caagtcagag gtgaaagccc ggggctcaac cccggaacag cctttgaaac taggtagcta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU92 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000092
VERSION     KHUT01000092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU92"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttta
       61 caggtccgtt gtgaaaactc gaggctcaac ctcgagacgc cggtggaaac cgtaaagcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU93 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000093
VERSION     KHUT01000093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU93"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           86 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatg
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcatagcta
      121 gagtacggta gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctgga cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU94 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000094
VERSION     KHUT01000094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU94"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaactc agggctcaac cctgagcgtg cttccgatac gggcagacta
      121 gaggtattca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg aattacctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU95 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000095
VERSION     KHUT01000095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU95"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggat gtgaaaaccc ggggcttaac cccgggcctg cattcgatac gggcagactc
      121 gagtgtggta ggggagactg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg ggtctctggg ccactactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU96 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000096
VERSION     KHUT01000096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU96"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcttta
       61 taagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tgtagagctt
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU97 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000097
VERSION     KHUT01000097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU97"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaattg cattggatac tggcaggcta
      121 gagtgcggta gaggatggcg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccatctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU98 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000098
VERSION     KHUT01000098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU98"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           84 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagta ttaaaagacg gtagcttaac tatcgggctg gtattgatac tgcagggctt
      121 gagtagagac gaggtaggcg gaattgataa tgtagcggtg aaatgcatag atattatcag
      181 gaactccgat tgcgaaggca gcttactagg ctcaaactga cgctgaggca cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01000099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU99 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000099
VERSION     KHUT01000099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU99"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          102 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggttcga
       61 taagtgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcaaac tgtcggactg
      121 gaggactgca gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag atatccggaa
      181 gaagaccggt ggcgaaggcg acctgctggg cagttactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000100
VERSION     KHUT01000100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c           97 g           52 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcttgt
       61 cacgtcgggt gtgaaagctt ggggcttaac tccaggtctg cattcgatac gggctggcta
      121 gaggtaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000101
VERSION     KHUT01000101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 cacgtaggag gcgagcgttg tccggaatca ttgggcgtaa agagcgcgca ggcggcttgg
       61 caagtcgggt gtgaaatccc ggggctcaac cccgtgactg catccgatac tgtcaggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000102
VERSION     KHUT01000102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tatttgtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca caattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000103
VERSION     KHUT01000103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctac
       61 taagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tggaaggctt
      121 gagtatggga gagggatgta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccattactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000104
VERSION     KHUT01000104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggttcgt
       61 cacgtcggga gtgaaaactc ggagctcaac tccgagcctg cttccgatac gggcagacta
      121 gaggtaggca ggggagagcg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000105
VERSION     KHUT01000105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           92 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggccaaa
       61 caggtccgtt gtgaaaactg gaggctcaac ctccagacgt cgacggaaac cgtgtggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000106
VERSION     KHUT01000106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           81 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctcct
       61 taagtcagtg gtgaaatact ctagctcaac tagaggggtg ccattgatac tgaggaactt
      121 gagtgaagtc gaagtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg cttatactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000107
VERSION     KHUT01000107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           84 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgcgcgta ggtggccaat
       61 taagtcaaat gtgaaatccc cgagcttaac ttgggaattg cattcgatac tggttggcta
      121 gagtatggga gaggatggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccgat ggcgaaggca gccatctggc ctaatactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01000108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000108
VERSION     KHUT01000108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcttcg
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tgcaaagctg
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000109             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000109
VERSION     KHUT01000109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           92 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggtgggtcgc
       61 ttagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tggcgatctt
      121 cgagtccggg agaggtgggt ggaactgcga gtgtagaggt gaaattcgta gatattcgca
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acactgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000110
VERSION     KHUT01000110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtgacg
       61 caagtctggt gtgaaatctc ggagctcaac tccgaaattg caccggatac tgcgtgactc
      121 gaggactgta gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg gatctctggg cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000111
VERSION     KHUT01000111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggccggt
       61 caagtgcggc gtgaaagtcc tgggctcaac ctggggacgg cgctgcggac tgttcggctg
      121 gagtccggaa gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggt ccggtactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000112
VERSION     KHUT01000112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggcttgg
       61 tatgtcgggt gtgaaatccc cgggctcaac ctgggaactg catccgaaac tgccttgctc
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccaatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000113
VERSION     KHUT01000113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcca
       61 caagtcacct gtgaaatctc cgggcttaac tcggagcctg caggcgaaac tgtggtgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000114
VERSION     KHUT01000114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcttgg
       61 caagtcgagt gtgaaatccc ttagcttaac taaggaatgg cgctcgaaac tacctcgcta
      121 gagggcggga gagggaagtg gaactctcgg tgtaggggtg aaatctgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctggc ccgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000115
VERSION     KHUT01000115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tacaaggcta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ttaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000116
VERSION     KHUT01000116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           93 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtctgct gtgaaatttc ggggctcaac cccgaacttg cagtgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccactactga cgctgagaag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000117
VERSION     KHUT01000117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggattgt
       61 taagcaagat gtgaaatccc cgggcttaac ctgggaatgg cattttgaac tggcagtcta
      121 gagtgcgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg atgacactga cgctcatgta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000118
VERSION     KHUT01000118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           93 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtctggt gtgaaagccc tgggctcaac ctgggaagtg cattggatac tggcaaactt
      121 gagtacggga gaggatagtg gaatttcgag tgtaggggtg aaatccgtag atattcgaag
      181 gaacaccggt ggcgaaggcg gctatctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000119
VERSION     KHUT01000119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttcgc
       61 taagtcagct gtgaaatccc cgggctcaac ctgggacttg cagttgaaac tggtgagcta
      121 gagtagggta gaggggtgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg acaccctgga ctcatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000120             251 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000120
VERSION     KHUT01000120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>251
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           92 g           44 t
ORIGIN      
        1 gacggggggg gcaagtgttc ttcggaatga ctgggcgtaa agggcacgta ggcggtgaat
       61 cgggttgaaa gtgaaagtcg ccaaaaactg gtggaatgct ctcgaaacca attcacttga
      121 gtgagacaga ggagagtgga atttcgtgtg taggggtgaa atccggagat ctacgaagga
      181 acgccaaaag cgaaggcagc tctctgggtc cctaccgacg ctggagtgcg aaagcatggg
      241 gagcgaacgg g
//
LOCUS       KHUT01000121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000121
VERSION     KHUT01000121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggttctc
       61 caagtccgtt gtgaaagccc cgggctcaac ctgggaatgg cagtggaaac taggggactt
      121 gaatctggca gaggggggtg gaatgccgcg tgtagcagtg aaatgcgtag agatgcggcg
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000122
VERSION     KHUT01000122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           89 g           52 t
ORIGIN      
        1 gacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggttatt
       61 caagtctttt gttaaatagt ggagctcaac tccatagagg cggaagaaac tgagtaactt
      121 gagtgcggta ggggtagagg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg ctctactggg ccgtaactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000123
VERSION     KHUT01000123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgc
       61 taagtcggtt gtgaaagccc ggggctcaac cccggagagt cgaccgatac tggcgagcta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000124
VERSION     KHUT01000124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcagaatga ctgggcgtaa agggcgtgta ggtggttgac
       61 taggtttgat gtgaaatccc cgggcttaac ctgggaattg cgtcgaaaac gggtcgactc
      121 gagtgagata gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg acaacctgga tcttaactga cactgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000125             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000125
VERSION     KHUT01000125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          100 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggtc gtgaaaactt ggggcttaac cctgagcttg cggtcgatac gggcatgact
      121 ggagttcggc aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000126
VERSION     KHUT01000126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           75 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatctgtctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacc cgaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000127
VERSION     KHUT01000127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtcggat gtgaaagctc ggagctcaac tccgaaatgg cattggaaac taccctgcta
      121 gaggattgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000128
VERSION     KHUT01000128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgtc gtgaaatcct ggggctcaac cccgggcgtg cgatggatac tgccgggcta
      121 gagggtggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccacacctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000129
VERSION     KHUT01000129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacggagggg actagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatttg
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tgcaaatctc
      121 gagtccggaa gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000130
VERSION     KHUT01000130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggtggtttct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggggaactt
      121 gagtgcagaa gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000131
VERSION     KHUT01000131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggcgatc
       61 tacgtctggg gtgaaagccc ggagctcaac tccgggactg ccttggatac gggatcgctt
      121 gaggattgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc caatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000132
VERSION     KHUT01000132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggatgta
       61 caagtctgct gtcaaagtgc agagcttaac tctgtaaagg cggtggaaac tgtaggtctt
      121 gagtatggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg ctctactggg ccataactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000133
VERSION     KHUT01000133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           88 g           53 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taagttagag gtgaaagccc ggagctcaac tccggaattg cctttaagac tgcatcgcta
      121 gaatcatgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000134
VERSION     KHUT01000134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           90 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggactat
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tggtagtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000135
VERSION     KHUT01000135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgg
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaattg cattcgatac tgcctgacta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggg ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000136
VERSION     KHUT01000136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaatgg cattggatac tggcagtcta
      121 gagtgcggta gagggtagcg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatccgt ggcgaaggcg gctacctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000137
VERSION     KHUT01000137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggctatg
       61 taagtcagga gtgaaagttt gcggctcaac cgtaaaattg cttttgatac tgcatggcta
      121 gaattaggat gaggtcagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccaat tgcgaaggca gctggctaga cctggattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000138
VERSION     KHUT01000138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           99 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtgctg
       61 cgcgtttggg gttacagctc ccggctcaac cgggagagcg cctcgaagac ggcagcactt
      121 gagggccaga gagggacacg gaactccggg tggagtggtg aaatgcgtag atatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tggtacctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000139
VERSION     KHUT01000139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaattg cattggatac tggcgggcta
      121 gagtacggta gagggtggcg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacatccgt ggcgaaggcg gccacctgga ccagtactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000140
VERSION     KHUT01000140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          102 g           41 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggccggt
       61 caagttaggc gtgaaaggcc tgggctcaac ctgggaacgg cgcttaagac tgatcggctg
      121 gagtccggga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctggc ccggcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000141
VERSION     KHUT01000141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcctct
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggc cattggaaac tgggaggctt
      121 gagtatagga gagaagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctttggc ctataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000142
VERSION     KHUT01000142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcggat gtgaaatccc caggctcaac ctgggaactg catccgatac tggatggctt
      121 gaatccggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000143
VERSION     KHUT01000143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           95 g           46 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattca ttgggcgtaa agagcgcgca ggcggcttgg
       61 taagtcggat gtgaaatccc gaggctcaac ctcgggtctg catccgatac tgcccggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac tgg
//
LOCUS       KHUT01000144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000144
VERSION     KHUT01000144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagaccgat gtgaaatccc cgggcttaac ctgggaactg cattggtgac tgcaaggctt
      121 gagtgtgtca gagggaggtg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca gcctcctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000145
VERSION     KHUT01000145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctatt
       61 taagctagat gtgaaagccc agggcttaac cttggaactg catttagaac tgggtagcta
      121 gagtacagca gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000146
VERSION     KHUT01000146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatgtg
       61 tcagtcaggg gtgaaatccc ggagctcaac ttcggaactg cctttgatac agcacgtctt
      121 gagtccgata gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactgga tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000147
VERSION     KHUT01000147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgct gtaaaagtca aaggctcaac ctttgaatgc cggtggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctagg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000148
VERSION     KHUT01000148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttgtt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaatgg cgatggaaac tgggcaacta
      121 gagtgtggta gagggtagtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaacatctgt ggcgaaggcg actacctgga ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000149
VERSION     KHUT01000149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           77 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggtttat
       61 taagtcaggg gtgaaagacg gcagcttaac tgtcgcagtg cctttgatac tgatgaactt
      121 gaatgaacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactatg gttttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000150
VERSION     KHUT01000150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           88 g           56 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaattg ccttcgatac tggctttcta
      121 gaggctggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccagatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000151
VERSION     KHUT01000151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagttagag gtgaaagccc ggggctcaac tccggaactg cctttaagac tgcatcgctt
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000152
VERSION     KHUT01000152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 caagaccgat gtgaaatccc cgggcttaac ctgggaatgg cattggtgac tgcacggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000153
VERSION     KHUT01000153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtctggg gtgaaagccc gctgctcaac agcggaactg ccctggatac tggagagctt
      121 gagtacagac gaggttggcg gaatggactg agtagcggtg aaatgcatag atacagtcca
      181 gaaccccgat tgcgaaggca gctgactagg ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000154
VERSION     KHUT01000154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccggc
       61 taggtctggt gtgaaaactc gaggctcaac ctcgagatgt cgccggaaac cagtcggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaagca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000155
VERSION     KHUT01000155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           38 c           73 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggacaa
       61 taagtcagcg gtgaaagtct gtggctcaac catagaattg ccattgatac tgttgttctt
      121 gagtgtttat gaagtggtta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca gatcactaat aaaatactga cgctgatgga cgaaagcgta
      241 ggtagcgaac agg
//
LOCUS       KHUT01000156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000156
VERSION     KHUT01000156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgt
       61 taagtcactt gtgaaatctc taagcttaac ttagaacggt caagtgatac tgatgtgcta
      121 gagtgcagaa ggggcaaccg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000157
VERSION     KHUT01000157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           91 g           43 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcacct
       61 caagtctgct gtcaaagacc ggggcttaac cccagaaagg cagtggaaac tgaagtgcta
      121 gagtgcagta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctctgctgga ctgcaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000158
VERSION     KHUT01000158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           86 g           57 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatatt
       61 taagtcaggg gtgaaatccc agagctcaac tctggaactg cctttgatac tgggtatctt
      121 gagtatggaa gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcttactggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000159
VERSION     KHUT01000159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           86 g           50 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggttttg
       61 aaagtttgct gtcaaagcgc ggagcttaac ttcgtaccgg cagtgaaaac tccaagacta
      121 gagtttggta ggggtcacgg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccagc ggcgaaagcg cgtgactggg cctgaactga cactcatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000160
VERSION     KHUT01000160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccgag
       61 taagtccgac gtgaaatctg gaggctcaac ctccagcggt cgttggaaac tactcggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gctctctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000161
VERSION     KHUT01000161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcttaa
       61 caagtcactt gtgaaatctc cgggcttaac tcggaacggc caagtgaaac tgtcaagcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000162
VERSION     KHUT01000162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggacttg
       61 taagtcggga gtgaaaactc ggggctcaac cccgagcctg cttccgaaac tgcgagtctt
      121 gaggaccgga gaggaaagtg gaattcctag tgtagcggtg gaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg actttctgga cggttcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000163
VERSION     KHUT01000163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           89 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctttg
       61 caagacagat gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcatggctg
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000164
VERSION     KHUT01000164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtccgg
       61 taagtccgct gtgaaagtcg ggggctcaac cctcgaatgc cggtggatac tgtcgggcta
      121 gagtgcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000165
VERSION     KHUT01000165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctga
       61 taagtagggg gtgaaatcct gcggcttaac cgcagggctg ccttctaaac tgtcaggctc
      121 gagcacagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcagt ggcgaaggcg gcctgctggg ctgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000166
VERSION     KHUT01000166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcacct gtgaaaactc tgggctcaac tcagagcctg caggcgaaac tgccgtgctt
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000167
VERSION     KHUT01000167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgcgc
       61 taagtcaggt gtgaaatttc ggagcttaac tccgaaactg catttgatac tggcgtgctt
      121 gaggactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000168
VERSION     KHUT01000168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcggac gtgaaatccc tcggcttaac cgaggaactg cgtccgatac tgaaaggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000169             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000169
VERSION     KHUT01000169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          105 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcccgg
       61 tcagtctttg gtgaaagcgc ggggctcaac cctgcgtcgg ccagggatac tgccgcggct
      121 ggagcactgt agaggcaggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcctgctgg gcagttgctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000170             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000170
VERSION     KHUT01000170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcagc
       61 acaagtcagt tgtgaaatct ccgggcttaa ctcggaaagg tcaactgata ctgtgcgact
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000171
VERSION     KHUT01000171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggcgacg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgagac tgcattgctg
      121 gagtacggaa gaggagggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccctctggt ccagtactga cactcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000172
VERSION     KHUT01000172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           58 c           85 g           47 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgttta ttgggcctaa agcatccgta gccggttctg
       61 caagtcttcc gttaaatcca cctgcttaac agatgggctg cggaagatac tatagagcta
      121 ggaggcggga gaggcaagcg gtactcgatg ggtaggggta aaatccgttg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000173
VERSION     KHUT01000173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           92 g           43 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggccctg
       61 taagtccgtt gtgaaagtca aaggctcaac ctttgaaagc cgatggatac tgcagggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000174
VERSION     KHUT01000174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctgct gtgaaaactc agggcttaac cctgagcttg cagtgggtac gggcagacta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000175
VERSION     KHUT01000175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccgttgatac tatctgtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacatg
      181 gaacaccaat tgcgaaggca gctcactaca cgaatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000176
VERSION     KHUT01000176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          103 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtcgact gtgaaaaccc ggggcttaac ttcgggcttg cagtcgatac gggcgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000177
VERSION     KHUT01000177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgca ggcggactga
       61 tcagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tgtcagtctc
      121 gagtccggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000178
VERSION     KHUT01000178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           99 g           46 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggatgg
       61 caagtcggtc gtgaaatccc tcggctcaac tgaggatcgt cgatcgatac tgccgttctt
      121 gaggattgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caattcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000179
VERSION     KHUT01000179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           90 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatgtg
       61 ccagtcaggg gtgaaatccc ggagctcaac tccggaactg cctctgatac agcacgtctt
      121 gagttcgaga gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000180
VERSION     KHUT01000180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtctgct gtgaaatccc cgggctcaac ctgggaatgg cagtggatac tggcaagcta
      121 gagtgtgata gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga tcaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000181
VERSION     KHUT01000181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c          100 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cgcgttgttc gtgaaaactc acagcttaac tgtgggcgtg cgggcgatac gggcagactg
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000182
VERSION     KHUT01000182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           41 c           75 g           57 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 taagtcaact gttaaatctt gaggctcaac ctcaaaatcg cagtcgaaac tattaggcta
      121 gagtatagta ggggtaaagg gaatttccag tggagcggtg aaatgcgtag agattggaaa
      181 gaacaccgat ggcgaaggca ctttactggg ctattactaa cactcagaga cgaaagctag
      241 ggtagcaaat ggg
//
LOCUS       KHUT01000183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000183
VERSION     KHUT01000183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           85 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttta
       61 taagtccgtg gtgaaagcca acagctcaac tgttgaactg ccatggatac tgtaaggctt
      121 gagtacagac gaggtaggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000184
VERSION     KHUT01000184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttgt
       61 taagttggat gtgaaatccc cgggctcaac ctgggaactg cattcaaaac tgacaagcta
      121 gagtatggta gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg accacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000185
VERSION     KHUT01000185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           84 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctta
       61 taagtcagtg gtgaaagccc atcgctcaac gatggaactg ccattgatac tgtaaggctt
      121 gagtacagac gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag ataccatcaa
      181 gaacaccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000186
VERSION     KHUT01000186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 tacagagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccacg
       61 cgtgtcttcc gtgaaatccc tcggctcaac cgaggacgtg cggaagaaac tgcgaggctc
      121 gagtccggaa gagggggacg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gtcccctggg ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000187
VERSION     KHUT01000187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtcag
       61 taagtcagtg gtgaaatctc tgagctcaac tcggaaactg ccattgatac tattggtctt
      121 gaatattgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000188
VERSION     KHUT01000188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           61 c           86 g           42 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggttctg
       61 caagtccccc gttaaatcca gctgctcaac agatgggccg cggaggatac tacagagcta
      121 ggaggcagga gaggcaagcg gtactcagtg ggtaggggta aaatccgttg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga atgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000189
VERSION     KHUT01000189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           93 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggtttta
       61 taagtcagat gtgaaagccc acggcttaac cgtggaagag catttgaaac tgtgagactt
      121 gagtaccaga ggggaaggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg accttctggc tggatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000190
VERSION     KHUT01000190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           66 c           88 g           39 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtcctg
       61 caagtcctcc gttaaatcca cccgctcaac ggttgggccg cgggggatac tacagggcta
      121 ggaggcggga gaggcaagcg gtactcgatg ggtaggggta aaatccactg atccattgaa
      181 gaccaccagt ggcgaaggcg gcctgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000191
VERSION     KHUT01000191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           96 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctctt
       61 cgcgtcggat gtgaaagctc aaagctcaac tttgagaggc cattcgatac gggagagcta
      121 gaggcaggta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctctctggg cctgttctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01000192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000192
VERSION     KHUT01000192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          107 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcacgca ggcggtgcgg
       61 cgcgtttcgc gtgacagcgg agggcttaac tctccgagga tgcgaaagac ggcggcactt
      121 gagggccaga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc tggtgactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000193
VERSION     KHUT01000193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtgagt
       61 taagtctgtc gtgaaagccc cgggctcaac ctgggaatgg cgatggatac tggctcgcta
      121 gagtgcggta gaggagagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatcagt tgcgaaggcg gctctctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000194
VERSION     KHUT01000194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggactct
       61 taagtcggtg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tgggagtctc
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000195
VERSION     KHUT01000195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttcg
       61 taagtctgtc gtgaaatccc cgggcttaac ctgggaatgg cgatggagac tgcgaggcta
      121 gagtttggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaaaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000196
VERSION     KHUT01000196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgccg
       61 taagtggggt gtgaaatccc ggggcttaac cccgggactg cattccatac tgcggtgctt
      121 gaggactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000197
VERSION     KHUT01000197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           77 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggtagg
       61 taagtcagtg gtgaaatctt caagcttaac ttggaaattg ccgttgatac tatttatctt
      121 gaatatcgtt gaggtttgcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacacctat tgcgaaggca gcaggctaaa cgaacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000198             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000198
VERSION     KHUT01000198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           48 t
ORIGIN      
        1 cacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtgtgt
       61 cgcgtcggcc gtgaaaacct gcagcttaac tgtgggcttg cggtcgatac gggcatcact
      121 ggagttcggc aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000199
VERSION     KHUT01000199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcacac gtgaaatccc tcggctcaac cggggaactg cgtgtgatac tggcgggctt
      121 gaattcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgagattga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000200
VERSION     KHUT01000200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 caagtcgcgt gtgaaagccc ccggctcaac tggggagggt cacgcgatac tgatcgactc
      121 gaaggcagga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000201
VERSION     KHUT01000201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           92 g           43 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 taagtccgct gtaaaagtca aaggctcaac ctttgaatgt cagtggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccaac ggcgaaggca gctcgctagg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000202
VERSION     KHUT01000202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtccgtg gtgaaagcca acagctcaac tgttgaactg ccatggatac tgacgggctt
      121 gagtacagac gaggtaggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000203
VERSION     KHUT01000203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           85 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtcagtg gtgaaagcct ccagctcaac tggagaactg ccattgatac tgacgggctt
      121 gagtacagac gaggttggcg gaattgattg tgtagcggtg aaatgcatag atacaatcaa
      181 gaactccgat tgcgtaggca gctaactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000204
VERSION     KHUT01000204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgtg
       61 taggtctgac gtgaaatctg gaggctcaac ctccagctgt cgttggaaac cgcgcggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000205
VERSION     KHUT01000205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcggat gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcaaggcta
      121 gagtcctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg accttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000206
VERSION     KHUT01000206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agggtgcgta ggtggtttgt
       61 taagtccgtt gtgaaatccc tgggctcaac ctgggaatgg cagtggatac tggcaagcta
      121 gagtgcggta gagggtagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000207             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000207
VERSION     KHUT01000207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcggct gtgaaattcc gaggctcaac ctcggaactg cagtcgaaac tattgtgact
      121 agagtccggt agaggagaat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000208
VERSION     KHUT01000208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           97 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctttg
       61 caagtgacgg gtgaaagccc tcggcttaac cgaggaactg cctgtcagac tgtgaggctt
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000209
VERSION     KHUT01000209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           94 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtttgc
       61 tcagtcagat gtgaaagccc tcggcttaac cggggacgtg catttgaaac tggcagactt
      121 gagtattgga gggggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg accacctggc caaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000210
VERSION     KHUT01000210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           91 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgtcg
       61 taagtcaggt gtgaaatctc ggggcttaac tccgaaactg catttgatac tgcggtgctt
      121 gaggactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000211
VERSION     KHUT01000211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggtccgg
       61 taagtcggtt gtgaaagccc agggctcaac cctgggatgc cgaccgatac tgtcggacta
      121 gagttcggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000212
VERSION     KHUT01000212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggtttt
       61 taagtcagtg gtgaaagccc acagctcaac tgtggaactg ccattgatac tgaagacctt
      121 gagtatcgtt gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat agcgtaggca gcttactaaa cgataactga cgctgaggca cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000213
VERSION     KHUT01000213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          107 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcat
       61 gaaggctgtg gtgaaagtcc ggggctcaac ctcggatcgg ccgtggcgac tcgtgggctg
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gcccgctggg cagtggctga cgctgaagcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000214
VERSION     KHUT01000214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           86 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgac gtgaaatctt caagctcaac ttggaaactg cgtcggatac tattcggcta
      121 gaggaatgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000215
VERSION     KHUT01000215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagttagag gtgaaagccc ggagctcaac tccggaactg cctttaagac tgcatcgctt
      121 gaacgtcgga gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acttactgga cgactgttga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000216
VERSION     KHUT01000216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggctggt
       61 caagtccgat gtgaaatctc ccggctcaac tgggaaactg catcggaaac tgatcggctt
      121 gaaggtggga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ccattcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000217
VERSION     KHUT01000217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c           98 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctgtt
       61 cgagtcggta gtgaaaactc aacgcttaac gttgggcttg ctatcgatac tgggcggctt
      121 gagtctggta ggggagtctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccagtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000218
VERSION     KHUT01000218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttgc
       61 cgtgtcctgt gtgaaagccc cgggctcaac ctgggaattg catgggaaac tggcaggctt
      121 gagtgtggca gaggagggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccctctggg ccaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000219             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000219
VERSION     KHUT01000219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           53 t
ORIGIN      
        1 tacgtagggg gctagcgtta tccggaatta ctgggcgtaa agggtgcgta ggtggtttct
       61 taagtcagag gtgaaaggct acggctcaac cgtagtaagc ctttgaaact gggaaacttg
      121 agtgcaggag aggagagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtt gcgaaggcgg ctctctggac tgtaactgac actgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01000220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000220
VERSION     KHUT01000220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcgggtttt
       61 taagtcagag gtgaaatcct ggagctcaac tccagaactg cctttgatac tgaaagtctt
      121 gagtatggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000221
VERSION     KHUT01000221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtccgtt gtgaaatctc gaggctcaac ttcgagcggt cgacggaaac catctggcta
      121 gagtccggaa gaggagagta gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaatacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000222
VERSION     KHUT01000222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccga
       61 ttagtcggct gtgaaagtcc agggctcaac cctgggatgc cggtcgatac tgtcggacta
      121 gagtccggaa gaggcaagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gcttgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000223
VERSION     KHUT01000223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           88 g           55 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtga
       61 caagtcaatt gtgaaatctc cgggcttaac tcggaacggt caattgatac tgttgtgcta
      121 gagtacagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgatactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000224
VERSION     KHUT01000224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          110 g           34 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 caaggcggcg gtgaaagtcc ggggctcaac cccggatcgg ccgtggcgac tgtcgcgctg
      121 gagcacagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000225
VERSION     KHUT01000225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgcc gtgaaaacct ggggctcaac cccaggcgtg cggtggatac tgccgggcta
      121 gagggtggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccatacctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000226
VERSION     KHUT01000226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggcgag
       61 cgagtcggaa gtgaaatctc ggggctcaac ttcgagactg ctcccgatac tgttcgtcta
      121 gagaccagta ggggttggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacacctgt ggcgaaggcg gccaactggg ctggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000227             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000227
VERSION     KHUT01000227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           47 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcatga
       61 caagtcactt gtgaaatctc tgggcttaac ccagaacggc caagtgatac tgtcgcgcta
      121 gagtgcggaa ggggcaatcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgagtgc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000228
VERSION     KHUT01000228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           90 g           51 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggtggttgtc
       61 caagttggat gtgaaagcct tgagctcaac tcaagaaatg cattcaggac tgggcggcta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cactaagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000229
VERSION     KHUT01000229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggtttgt
       61 taagtctgat gtgaaagccc cgggctcaac ccgggaagtg cattggaaac tggctgactt
      121 gagtacggga gaggaaagtg gaatttcgag tgtaggggtg aaatccgtag atattcgaag
      181 gaacaccagt ggcgaaggcg gctttctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000230
VERSION     KHUT01000230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggacctg
       61 caagtcgggg gtgaaagccc gaggctcaac ctcggaactg ccttcgatac tgcgggtctc
      121 gagtccggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000231
VERSION     KHUT01000231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaatt
       61 caagtcagct gtgaaatctc cgagcttaac tcggaaaggt cagctgatac tgctttgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000232
VERSION     KHUT01000232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggagtgg
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatcattctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcttgctaca cgatcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000233
VERSION     KHUT01000233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taagtctgct gtgaaaactc gaggctcaac ctcgagctgt cggcggaaac tatttggcta
      121 gagtccggaa gaggaaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctttctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000234             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000234
VERSION     KHUT01000234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttgt
       61 gtagtcccat gtcaaagacc aggacttaac cctgggccgg catgtggaaa ctcgcaaggt
      121 tgagtacggt aggggcaggg ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 agaacaccaa tggcgaaagc actctgctgg gccgaaactg acactaagag acgaaagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01000235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000235
VERSION     KHUT01000235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggtcgt
       61 taagtcaggg gtgaaatccc agagctcaac tctggaactg cctttgatac tggcgaccta
      121 gagtccggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000236
VERSION     KHUT01000236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtga
       61 caagtcactt gtgaaatctc tgagcttaac tcagaacggc caagtgaaac tgttgtgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000237
VERSION     KHUT01000237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c           97 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaactt ggggcttaac cccattcgtg cttccgatac gggcagacta
      121 gaggcaggca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgttctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000238
VERSION     KHUT01000238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          103 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgtcg
       61 caagtcggct gtgaaagccc ggggctcaac cccggaggtg cagccgagac tgcggtgctc
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000239
VERSION     KHUT01000239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcgcg
       61 caagtcggat gtgaaatctc agggctcaac cctgaaactg cattcgatac tgcgtggcta
      121 gagtgctgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagcaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000240
VERSION     KHUT01000240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           81 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcata
       61 taagtcagcg gtgaaatccg gcagctcaac tgtcgatgtg ccattgatac tgtatgactt
      121 gagtactgat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaag cagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000241
VERSION     KHUT01000241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggcggct
       61 tgtgtcaggt gtgaaatccc cgggcttaac ctgggaattg cgcttgaaac taggtcgcta
      121 gagttcatta gaggggggtg gaattccagg tgtagcagtg aaatgcgtag agatctggag
      181 gaacaccgat ggcgaaggca gccccctggg gtgaaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000242
VERSION     KHUT01000242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcacct gtgaaacctc taggcttaac ctagagcctg caggcgaaac tgccgtgctt
      121 gagggtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacatctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000243
VERSION     KHUT01000243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agcgcgcgta ggcggccgac
       61 cacgtcggat gtgaaagctc agggctcaac cctgagaggc cattcgatac gggtcggcta
      121 gaggcaggta ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgttctga cgctgaggtg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01000244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000244
VERSION     KHUT01000244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcagag gtgaaatccc ggagctcaac ttcggaactg cctttgatac tggcaatcta
      121 gagtccggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000245
VERSION     KHUT01000245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggatcgt
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tggcgatctc
      121 gagtccggaa gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000246
VERSION     KHUT01000246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggtggctgag
       61 tcagtcgatt gtgaaagccc tgggcttaac ctgggaattg cagtcgatac tactcagcta
      121 gagtatggga gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctacctggc ccaatactga cactcaggtg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000247
VERSION     KHUT01000247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggtctgt
       61 caagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgaaac tggcaggcta
      121 gagtcttgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccggt ggcgaaggcg gccccctgga caaagactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000248             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000248
VERSION     KHUT01000248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          101 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccaag
       61 gcagtcggct cgtgaaagcc cggggctcaa ccccggaggt gcggccgata ctctttggct
      121 cgaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggtcctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000249
VERSION     KHUT01000249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccct
       61 taagtcagtg gtgaaagccc gcggctcaac cgcggaactg ccattgatac tggggggctt
      121 gagtacagac gaggtaggcg gaattgaaga tgtagcggtg aaatgcttag atatcttcaa
      181 gaacaccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000250
VERSION     KHUT01000250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          105 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcggga
       61 caagcgtgcg gtgaaagcct ggggctcaac cccgggtcgg ccgtgcgaac tgttttgctt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000251
VERSION     KHUT01000251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           85 g           60 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtgggcttc
       61 taagtcggtg gtgaaatcct gcagcttaac tgtagaactg ccattgatac tggaagtctt
      121 gagtacggca gagggagata gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaataccagt tgcgaaggcg gtctcctggt ccgttactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000252             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000252
VERSION     KHUT01000252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcacgt gtgaaatctc cacgctcaac gtggaggcgc cacgtgagac tgccgtgact
      121 tgagtccggt agaggagcgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000253
VERSION     KHUT01000253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           61 c           84 g           45 t
ORIGIN      
        1 taccagcacc ccgagttgtc gggacgatta ttgggcctaa agcatccgta gcctgttctg
       61 caagtcctcc gttaaatcca cctgctcaac ggatgggctg cggaggatac cgcagagcta
      121 ggaggcggga gaggcaaacg gtactcagtg ggtaggggta aaatccattg atctactgaa
      181 gaccaccagt ggcgaaggcg gtttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000254
VERSION     KHUT01000254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtttgt
       61 cgcgtccgtt gtgaaagctt ggggctcaac cccaagtctg cagtggatac gggcagacta
      121 gagtcaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggaa cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000255
VERSION     KHUT01000255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 tacagagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggcctgt
       61 taagtcgaac gtgaaatccc cgggctcaac ccgggaactg cgtccgagac tggcaggctc
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000256
VERSION     KHUT01000256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           91 g           46 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcttat
       61 caagtctgct gtcaaagagt ggggcttaac cccattaagg cagtggaaac tgagaagcta
      121 gagtgccgta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga cggcaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000257
VERSION     KHUT01000257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttct
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg cgtttgaaac tgggagactt
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000258
VERSION     KHUT01000258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgc
       61 caagtcaggt gtgaaatccc cgggcttaac ctgggaattg cgcttgaaac tagcgggctt
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000259
VERSION     KHUT01000259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctgt
       61 tatgtcggat gtgaaagccc ttggcttaac tgaggaagtg catccgaaac tggcaggcta
      121 gagtactaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000260
VERSION     KHUT01000260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcactt gtgaaatctt caggcttaac ctgaagcggc caagtgatac tgtcgtgcta
      121 gagtgcggaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000261
VERSION     KHUT01000261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 caggtccgtt gtgaaaactc gaggctcaac ctcgagacgt cgacggaaac cgtctggcta
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gcactctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000262
VERSION     KHUT01000262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtttgta ggtggcttgt
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tgatgagctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgagaaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000263
VERSION     KHUT01000263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           92 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttctt
       61 taagtttgct gtgaaatctc ccggcttaac cgggagggtg cggtgaatac tgagggacta
      121 gagtacggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000264
VERSION     KHUT01000264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggctag
       61 taagtccgtg gtgaaatctc tgagcttaac tcggaaactg ccgtggatac tattagtctt
      121 gaatctcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttgctaca cgagtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000265
VERSION     KHUT01000265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          100 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcccgt
       61 taagtcggtt gtgaaaaccc gaggctcaac ctcgggcctg catccgatac tggcgggctt
      121 gaggcaggta ggggagagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01000266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000266
VERSION     KHUT01000266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgg
       61 tcagttaggg gtgaaatccc agggctcaac cctggaactg cctttaatac tgccggtcta
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000267
VERSION     KHUT01000267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgc
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgtaac tggcaagcta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000268
VERSION     KHUT01000268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           85 g           49 t
ORIGIN      
        1 tacgagaggt ccaaacgtta ttcggaatta ctgggcttac agagttcgta ggcggctcta
       61 caagtgaggt gtgaaagccc tcggctcaac cgaggaactg cgcttcaaac tgtagaacta
      121 gagggagata gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000269
VERSION     KHUT01000269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           88 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgca ggcggattga
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaattg cctttgatac tgtctgtctt
      121 gagtccggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000270
VERSION     KHUT01000270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcga
       61 caagtcactt gtgaaatctc tgggctcaac ccagaacggt caagtgatac tgtcgtgcta
      121 gagtgcggaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000271
VERSION     KHUT01000271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          105 g           41 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggtggctcat
       61 tacgcccggc gtgaaagccc ccggcctaac cggggaaggt cgtcgggtac gggtgagctt
      121 gagggtatca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg atacacctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000272
VERSION     KHUT01000272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           89 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcttgg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tcccgagctc
      121 gagtaccgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt agcgaaggcg gccttctgga cggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000273
VERSION     KHUT01000273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           64 c          111 g           36 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcggtg
       61 ctggcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac cgcaccgctt
      121 gagggcggta ggggctggtg gaatgcctgg tgtagtggtg aaatgcgtag agatcaggcg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgtccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000274
VERSION     KHUT01000274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtcggtt gtgaaaactc gaggctcaac ctcgagatgt cgaccgaaac catctggcta
      121 gagtccggaa gaggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000275
VERSION     KHUT01000275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           85 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcctg
       61 taagtcagtt gtgaaatacg gcagcttaac tgtcgaggtg caattgatac tgcgggacta
      121 gagtactgat gaggtaagcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000276
VERSION     KHUT01000276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           75 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc caggcttaac ttggaaactg ccattgatac tatcaatctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000277
VERSION     KHUT01000277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagttcgta ggcggtttgt
       61 cgcgtcgtgc gtgaaaactt ggggcttaac tccaagcgtg cgggcgatac gggcagactt
      121 gagtactaca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg tagtaactga cgctgaggaa cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000278             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000278
VERSION     KHUT01000278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttact
       61 taagtcaggt gtgaaagccc tgggcttaac ctaggaattg cgcttgagac tgggtagctt
      121 tgagtgtggc agagggaggt ggaattccac gtgtagcagt gaaatgcgta gagatgtgga
      181 ggaacaccga tggcgaaggc agcctcctgg gccagcactg acgctcatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000279
VERSION     KHUT01000279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtcagcg gtgaaatccc agggctcaac cctggaactg ccgttgatac tggcgggctt
      121 gagttcggtc gaggcgggcg gaactggtgg tgtagcggtg aaatgcatag ataccaccaa
      181 gaaccccgat tgcgtaggca gctcgctagg ccgaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000280
VERSION     KHUT01000280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           88 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcttgg
       61 caagtcggat gtgaaatccc tcggctcaac caaggaagtg catccgaaac tgctgagctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000281
VERSION     KHUT01000281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccga
       61 taagtccgct gtgaaagtcc agggctcaac cctggaatgc cggtggatac tgtcgggctc
      121 gaatccggaa gaggtgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcactggg acggtattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000282
VERSION     KHUT01000282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           82 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgg
       61 aaagaaagat gtgaaatccc agagcttaac tttggaactg catttttaac tgccaagcta
      121 gagtatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000283
VERSION     KHUT01000283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtctgat gtgaaatctt gcggctcaac cgcaagcggt cattggaaac tgggggactt
      121 gagtacagaa gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000284
VERSION     KHUT01000284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggtggccgcg
       61 caagtcgagt gtgaaatccc cgagcttaac ttgggaattg cgctcgaaac tacgtggctc
      121 gagtgtggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccttctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000285
VERSION     KHUT01000285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggatata ctgggcgtaa agcgcacgta ggcggcgccg
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cccttgaaac tgcagtgcta
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000286
VERSION     KHUT01000286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           95 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgtgca ggcggctccg
       61 gaagtctgat gtgaaagccc cgggcttaac ccgggaagtg cattggaaac tccggggctt
      121 gagtgctgga gaggaaggga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cccttctgga cagcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000287
VERSION     KHUT01000287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           53 c          103 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggtcacg
       61 cacgtccgtt gtgaaatcgc tcggctcaac tgggcggggt cagcggatac ggcgtggctg
      121 gagcaagcta gggggcaatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctgga gctttgctga cgctgatgcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01000288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000288
VERSION     KHUT01000288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           86 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtcggat gtgaaagctc cgagctcaac tcggaaatgg cattggaaac taccttgctc
      121 gagggtttga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagta
      181 gaacaccagt ggcgaaggcg agtccctgga aaactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000289
VERSION     KHUT01000289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggatagg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccgttgatac tatttatctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactacg cggtcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000290
VERSION     KHUT01000290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 caggtccgtt gtgaaaacta gaggcttaac ctctagacgt cgatggaaac cgtttggcta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggctaaggcg gttctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000291
VERSION     KHUT01000291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgc
       61 taagtctgtg gtgaaatccc ggggctcaac cccggaactg ccctggatac tggcgggctt
      121 gagttcggtt aaggcgggcg gaactggcgg tgtagcggtg aaatgcatag ataccgccaa
      181 gaaccccgat tgcgtaggca gctcgctgag ccgaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000292
VERSION     KHUT01000292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           43 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggatgg
       61 caagtcagtc gtgaaatctc tcggctcaac cgagaaacgt cggctgatac tgccgttctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg catctcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000293
VERSION     KHUT01000293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggtgtg
       61 caagtcggaa gtgaaatccc ggggctcaac tccggggctg ctgccgatac tgcacgtcta
      121 gagaccagta gaggttggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacacctgt ggcgaaggcg gccaactggg ctggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000294
VERSION     KHUT01000294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           86 g           59 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcttta
       61 taagtcagtg gtgaaataca gccgctcaac ggttgaggtg ccattgatac tgtagagctt
      121 gaaacaagtg gaggctgccg gaatggatgg tgtagcggtg aaatgcatag atatcatcca
      181 gaacaccgat tgcgaaggca ggtggctacg tttgatttga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01000295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000295
VERSION     KHUT01000295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatact ttcgcttaac gaaggaactg ccattgatac taccagtctt
      121 gaatacagtg gaggtaggcg gaatgcgtca tgtagcggtg aaatgcttag atatgacgca
      181 gaacaccaat tgcgaaggca gcttgctaca ctgttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000296
VERSION     KHUT01000296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           83 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatatt tcagctcaac tggaattgtg ccattgatac tgcggggctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000297
VERSION     KHUT01000297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggacatt
       61 taagtcagag gtgaaatccc ggagcttaac ttcggaactg cctttgatac tgggtgtctt
      121 gagtgtgaga gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggc tcattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000298
VERSION     KHUT01000298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtccgct gtgaaagccc tgggctcaac ctgggaattg cagtggatac tggcaagcta
      121 gagtgcggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccatctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000299
VERSION     KHUT01000299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           95 g           41 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggccccg
       61 caagtcaggc gtgaaatccc cgggctcaac ttgggaatgg cgcttgaaac tacggggctg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000300
VERSION     KHUT01000300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgttg
       61 taagtcacct gtgaaacctc tgggcttaac tcagagcctg caggcgaaac tgcaatgctg
      121 gagggtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccactactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000301
VERSION     KHUT01000301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagttagag gtgaaagccc ggggctcaac cccggaactg cctttaaaac tgcatcgctt
      121 gaatccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggcattga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000302
VERSION     KHUT01000302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           89 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtgatg
       61 taagacagtt gtgaaatccc cgggctcaac ctgggaactg catctgtgac tgcatcgctg
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000303
VERSION     KHUT01000303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           96 g           41 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgc
       61 taagtcgaac gtgaaatccc cgagctcaac tcgggaactg cgtccgatac tggcgggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000304
VERSION     KHUT01000304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgctg
       61 taagtctgtg gtgaaatctc tgggctcaac tcagagtctg ccatagaaac tgcagtgctg
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000305             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000305
VERSION     KHUT01000305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcggcg gtgaaatacc gaggctcaac ctcggcactg ccgtcgatac tgctatggct
      121 agagtttggt agaggagaac ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacacccg tagcgaaggc ggttctctgg gccaatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000306
VERSION     KHUT01000306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           94 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcgtct gtgaaatcct ccagcttaac tgggggcgtg caggcgatac gggcagactt
      121 gagtactaca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg tagtaactga cgctgaggag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000307
VERSION     KHUT01000307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccttg
       61 caagtcaggc gtgaaagccc tcggcttaac cggggaagtg cgtctgaaac tgcttggctt
      121 gagtacggga gagggaggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gcctcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000308
VERSION     KHUT01000308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           42 c           79 g           52 t
ORIGIN      
        1 tacgggggat gcaagtgtta tccggaataa ttgggcgtaa agcgtctgta ggtggttgac
       61 caagtctgtt gttaaaaatc agggcttaac cctgatccgg caacagaaac tagtaaactt
      121 gagtacggta ggggcagagg gaattctcgg tgtagtggtg aaatacgtag atatcgagaa
      181 gaacaccaat agcgaaagca ctctgctggg ccgaaactga cattgagaga cgaaagctag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000309
VERSION     KHUT01000309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           99 g           40 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggtgtg
       61 caagtcggaa gtgaaatctc ggggctcaac tccgagactg ctcccgatac tgcggacctg
      121 gagaccggta gaggtgggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacacctgt ggcgaaggcg gcccactggg ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000310
VERSION     KHUT01000310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttt
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cctctgaaac tggatggcta
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000311
VERSION     KHUT01000311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           94 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacat
       61 ttagtcaggc gtgaaagccc cgggctcaac ctgggaattg cgtttgaaac tggtgagctt
      121 gaggaccgga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000312
VERSION     KHUT01000312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacggaggga gctagcgtta ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtaagag gtgaaagccc agagctcaac tctggaattg ccttttagac tgcatcgctt
      121 gaatcatgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctgactgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000313
VERSION     KHUT01000313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttgt
       61 taagtcaggg gtgaaagacg atggctcaac catcgcagtg cctttgatac tggcgagctt
      121 gaatgtactt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactaaa gtatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000314
VERSION     KHUT01000314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           99 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctga
       61 taagcagagg gtgaaatcct gtggctcaac cacagggctg ccttctgaac tgtcaggctg
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000315
VERSION     KHUT01000315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccgat
       61 caagtcagag gtgaaagccc aaggctcaac cttggaattg cctttgatac tgttcggctc
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000316
VERSION     KHUT01000316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           89 g           46 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggtggctcta
       61 taagtccgtt gtgaaagtca aaggctcaac ctttgaaagc cgatggatac tgtagagcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgtgactga cactaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000317
VERSION     KHUT01000317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 caagtcacct gtgaaacctc tgggcttaac ccagagcctg caggcgaaac tgccgtgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000318
VERSION     KHUT01000318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          102 g           39 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctgtg
       61 gcagtcgggt gtgaaagccc ggagctcaac tccggaacgg cactcgagac gccacggctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000319             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000319
VERSION     KHUT01000319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           51 t
ORIGIN      
        1 tacgtagggg gctagcgtta tccggaatta ctgggcgtaa agggtgcgta ggcggtcttt
       61 caagccagaa gtgaaaggct acggctcaac cgtagtaagc ttttggaact gtaagacttg
      121 agtgcaggag aggagagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagta gcgaaggcgg ctctctggac tgtaactgac gctgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01000320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000320
VERSION     KHUT01000320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggctatt
       61 taagtcagat gtgaaagccc tgggcttaac ctgggaactg catttgaaac tggatggcta
      121 gagtacagca gaggacggtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg accgtctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000321
VERSION     KHUT01000321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctt tgggcttaac ccagaaactg ccattgatac tatctgtctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000322
VERSION     KHUT01000322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacggagggg gccagcgttg ctcggaatta ttgggcgtaa agggcgcgta ggcggtctgg
       61 taagtttgat gtgaaatccc atggcttaac catggaactg catctcatac tgccgggcta
      121 gagtctggga gaggggagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctccctgga ccagtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000323
VERSION     KHUT01000323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcgaac gtgaaatccc cgggctcaac ccgggaactg cgttcgagac tggtgggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000324
VERSION     KHUT01000324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           89 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcattt
       61 caagtctgct gtcaaagacc ggggcttaac cctggaaagg cagtggaaac tgaagcgcta
      121 gagtgtcgta ggggtagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacatcggt ggcgaaagcg ctctactgga cgacaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000325
VERSION     KHUT01000325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttt
       61 taagtcagtg gtgaaagccc atagctcaac tatggaactg ccattgatac tgaaaggctt
      121 gagtacagac gaggttggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaactccgat tgcgtaggca gctaactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000326
VERSION     KHUT01000326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           76 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtccgtg gtgaaatctc caagcttaac ttggaaactg ccatggatac tataggtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cgaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000327
VERSION     KHUT01000327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           99 g           46 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtac agggagcgta ggcggttgga
       61 taagccctcc gtgaaatctc caggcttaac ctggaaagtg cagaggggac tgttcagcta
      121 gaggatggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acatttctga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000328
VERSION     KHUT01000328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgtgac tgcacggcta
      121 gagtacggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000329
VERSION     KHUT01000329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          106 g           41 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgtgcgca ggcggctcat
       61 cgcgctcggc gtgaaagccc ccggctcaac cggggagggt cgtcgaggac gggtgagctt
      121 gagggtatca ggggttggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccaactggg atacacctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000330
VERSION     KHUT01000330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          102 g           45 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgacc gtgaaaaccc gtggctcaac tacgggctgg cggtcgatac gggcaggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000331
VERSION     KHUT01000331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 tacagagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctttg
       61 cgtgtctccc gtgaaatccc tcggctcaac cgaggacgtg cggaagaaac tgcaaggctc
      121 gagtccggaa gaggggggcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gtcccctggg ccggaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000332             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000332
VERSION     KHUT01000332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtcct
       61 gtaagtcgga agtgaaattt cggagctcaa ctccgaagct gcttctgata ctgcggatct
      121 agggatcggt agaggtcggt ggaattacag gtgtagcggt ggaatgcgta gatatctgta
      181 agaacacccg tggcgaaggc ggccgactgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000333
VERSION     KHUT01000333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggtttg
       61 taagtagggg gtgaaatccc agggctcaac cctggaactg ccttctaaac tgcaagcctg
      121 gaggtcagga gaggcgagtg gaataccgag tgtagaggtg aaattcgtag atattcggtg
      181 gaacaccagt ggcgaaggcg actcgctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000334
VERSION     KHUT01000334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctgct gtgaaaatcc agggctcaac cctggacctg cagtgggtac gggcagacta
      121 gagtgcggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000335
VERSION     KHUT01000335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtctgtc gtgaaagccc cgggctcaac ctgggaatgg cgatggatac tggcaagcta
      121 gagtgtgtca gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccatctggg acaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000336
VERSION     KHUT01000336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggccttt
       61 taagtctgat gtgaaagccc acggctcaac cgtggaaggt cattggaaac tgaaaggctt
      121 gagtacagaa gaggaaagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctttctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000337
VERSION     KHUT01000337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           81 g           63 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattaa
       61 taagtcagtg gtgaaatact gcagcttaac tgtagaattg ccgttgatac tgttagtctt
      121 gagtacggtc aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactggg ccattactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000338
VERSION     KHUT01000338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           76 g           57 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtccact gttaaagatt gaagcttaac ttcaaaaatg ccgtggaaac ttttaaactt
      121 gagtatgata ggggtagagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaccaccaat ggcgaaggca ctctactggg ccattactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01000339             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000339
VERSION     KHUT01000339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaaat
       61 taagtcatct gtgaaatctc cgagcttaac tcggaacggt caggtgatac tgatctgcta
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgacagc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000340
VERSION     KHUT01000340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 taagttcgtt gtgaaagccc cgggctcaac ctgggaacgg caatggaaac tggcgggcta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca accccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000341
VERSION     KHUT01000341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttcgg
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaaaac tggcgaactg
      121 gagagtggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccatttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000342
VERSION     KHUT01000342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttctg
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg cgtttgaaac tacaggacta
      121 gagtatggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccaat ggcgaaggca gccccctggg tcaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000343             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000343
VERSION     KHUT01000343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           92 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggctaat
       61 caagtctgcg gtcaaagact ggggcttaac cctggaaagg ccgtggaaac tgatagagct
      121 agagtaccgt aggggtagag ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 agaacatcgg tggcgaaagc gctctactgg acggtgactg acactcaggg acgaaagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01000344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000344
VERSION     KHUT01000344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccaac
       61 taagtcggac gtgaaatccc tcagcttaac tggggaactg cgtccgatac tggatggctt
      121 gagtttggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaacactga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000345
VERSION     KHUT01000345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           96 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctggt gtgaaatccc atggctcaac tgtgggcttg catcgggtac gggcagacta
      121 gagtgctgta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg cagttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000346
VERSION     KHUT01000346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtcagct gtgaaagtca agggctcaac ccttgaatgc cggttgatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000347
VERSION     KHUT01000347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgcc
       61 taagtcagac gtgaaatccc tcggctcaac cggggaactg cgtctgatac tgggtggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgagattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000348
VERSION     KHUT01000348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggactgt
       61 taagtcggac gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tagcagtctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcggcctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000349
VERSION     KHUT01000349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           91 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcggag
       61 taagttgggt gtgaaatccc ggggcttaac ctcggaattg cattcaatac tgcttcgctc
      121 gagtactgga gaggaggctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaaggccggt ggcgaaagcg agcctctgga cagttactga cgctgatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000350
VERSION     KHUT01000350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           76 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggttttg
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgcaagactt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactacc aatatattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000351
VERSION     KHUT01000351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           88 g           47 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggaccgg
       61 caagttggat gtgaaatctc ctggctcaac tgggagaggc cgttcaaaac taccggtctt
      121 gaggatggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga ccattcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000352
VERSION     KHUT01000352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcgcgt
       61 taagacaggt gtgaaagccc cgagcttaac ttgggaattg cgcttgtgac tggcgagctg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccagcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000353
VERSION     KHUT01000353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           88 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcta
       61 taagacagat gtgaaatccc cgggctcaac ctgggaactg catttgtgac tgtagagctg
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000354
VERSION     KHUT01000354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcacct gtgaaatctc ctggctcaac tgggagcttg caggcgaaac tgccgtgctg
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000355
VERSION     KHUT01000355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgc
       61 taagacagat gtgaaatccc cgggcttaac ctgggaactg catttgtgac tggcgggcta
      121 gagtatggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000356
VERSION     KHUT01000356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacggggggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctagg
       61 taagtccggg gtgaaagacg gccgctcaac ggtcgcaggg ccctggatac tgcccagctc
      121 gagtgcggat gaggtgggcg gaatctacga agtagcggtg aaatgcatag atatcgtaga
      181 gaactccgat tgggaagcca gctcactggg ccgttactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000357
VERSION     KHUT01000357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgacc gtgaaaactt ggggctcaac cccaagcctg cggtcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000358
VERSION     KHUT01000358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           83 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gtcggttatc
       61 taagtcaggt gtgaaatccc cgggctcaac ctgggaattg catttgatac tggatagcta
      121 gagttcggca gagggaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000359
VERSION     KHUT01000359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           79 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccgttgatac tatctgtctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactaca cgatcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000360
VERSION     KHUT01000360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggatggt
       61 tgcgtccgga gtgaaatttc gccgctcaac ggcgaagctg ctttggatac ggaccgtcta
      121 gggaccggta gaggctggta gaattgcagg tgtagcggtg gaatgcgtag agatctgcaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000361             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000361
VERSION     KHUT01000361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcttgt
       61 taagtccggg gtgaaaggca gtggctcaac cacttgcagg gccctggata ctggcaggct
      121 cgagtgtagg tgaggccggt ggaacgggtg gtgtagcggt gaaatgcata gataccatcc
      181 agaacaccga ttgcggaggc agccggctaa cctactactg acactgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000362
VERSION     KHUT01000362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggattta ctgggcgtaa agcgtgtgta ggcggcttgg
       61 caagttggat gtgaaagccc acggctcaac cgtggaagcg catccaaaac tgcttcgctc
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacacctgc ggcgaaggcg gccttctgga cgatgactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000363
VERSION     KHUT01000363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgagta ggcggcgttc
       61 taagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tgggaggcta
      121 gagtatggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccataactga cgctgatccg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000364
VERSION     KHUT01000364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           88 g           44 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgag
       61 tgtgtgaggt gtgaaagccc ggggcttaac cccggaaatg cgcctcaaac tacacggcta
      121 gagcattgga gagggtagca gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaataccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000365
VERSION     KHUT01000365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgtgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccatgcta
      121 gaggattgga gaggtagctg gaattcttgg tgtagcagtg aaatgcgtag agatcaagag
      181 gaacactcgt ggcgaaagcg agctactgga caattcctga cgctgaggct cgaaggctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01000366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000366
VERSION     KHUT01000366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcacgta ggctggctgt
       61 taagtcgggg gtgaaatcct gaggctcaac ctcagaattg ccttcgatac tggcggtctc
      121 gagtgcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgcaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000367
VERSION     KHUT01000367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgaagggg actagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggatcgc
       61 taagtcaggg gtgaaatccc agggctcaac cctggaactg cctttgatac tgacgatctg
      121 gagatcggga gaggttggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gccaactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000368
VERSION     KHUT01000368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           75 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggacag
       61 taagtcagtg gtgaaatcct atcgcttaac gatagaattg ccattgatac tgctgacctt
      121 gagtacaaat gatgtgggcg gaatgtgcag tgtagcggtg aaatgcttag atattgcaca
      181 gaacaccgat agcgaaggca gctcacaaaa atgtaactga cgctgaggca cgaaagtatg
      241 gggatcaaac agg
//
LOCUS       KHUT01000369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000369
VERSION     KHUT01000369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgtgt
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaaccg cgcttgagac tggcacgctc
      121 gagttcggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgat ggcgaaggca gccatctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000370
VERSION     KHUT01000370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctaag
       61 taagtcgggc gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tgcatagcta
      121 gagtcctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000371
VERSION     KHUT01000371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgtc gtgaaatcct gaggctcaac cttggacgtg cgatggatac tgccgggcta
      121 gaggatggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccattcctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000372
VERSION     KHUT01000372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgc
       61 taagtcgaac gtgaaatccc caggctcaac ctgggaactg cgtccgatac tggcaggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000373
VERSION     KHUT01000373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cacgtcggat gtgaaagccc ggggcttaac cccgggtctg cattcgatac gggcagacta
      121 gagtgtggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000374
VERSION     KHUT01000374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           73 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtagg
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccattgatac tatttatctt
      121 gaatactctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca tagttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000375
VERSION     KHUT01000375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           58 c          107 g           41 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcggta
       61 taagcatggc gtgaaagccc caggctcaac ctgggagggt cgtcgtggac tgtaccgctt
      121 gagggcggta ggggctggtg gaatgcctgg tgtagtggtg aaatgcgtag agatcaggcg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgtccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000376
VERSION     KHUT01000376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           85 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgatgggctt
      121 gagtggagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gcttactagt ctcaaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000377
VERSION     KHUT01000377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           82 g           64 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttttg
       61 taagtcagtg gtgaaatata gcagcttaac tgttagggtg ccattgatac tgcagaactt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000378
VERSION     KHUT01000378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgc
       61 taagttggat gtgaaaactc tgggcttaac ccagagcctg catccaaaac tggcaaactt
      121 gagtactgga ggggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000379
VERSION     KHUT01000379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtgggg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgccttgctc
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000380             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000380
VERSION     KHUT01000380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta tccggaatca ttgggcgtaa agagctcgta ggcggttcaa
       61 caagtcggct gtgaaagtcc ggggctcaac cccggaattg cagtcgatac tgttgtgact
      121 tgagtccggt agaggagaat ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000381
VERSION     KHUT01000381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggatcag
       61 gaagttgggg gtgaaatccc ggggctcaac cccggaatgg ccttcaaaac tcctggtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000382             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000382
VERSION     KHUT01000382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcggtc gtgaaaaccc ggggctcaac cccgggaggc cggtcgatac tgctgtggct
      121 agggtccggt agaggagagt ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggctctctgg gccggtaccg acgctgagga gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000383
VERSION     KHUT01000383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           97 g           46 t
ORIGIN      
        1 tacgtaggga ccgagcgtta tccggattta ttgggcgtaa agcgcgcgta ggcggccaca
       61 cacgtcggtt gtgaaagctc ggagctcaac tctgagaggc cttccgatac ggtgtggcta
      121 gaggtaggta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01000384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000384
VERSION     KHUT01000384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 aaagtctgat gtgaaatcct ggggctcaac cctggaactg cattggatac ttccttgcta
      121 gagtactgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000385
VERSION     KHUT01000385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggttcgt
       61 cgcgtcgttt gtgaaaactt ggggctcaac cttaagcttg caggcgatac gggcggacta
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgagaag cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000386
VERSION     KHUT01000386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggtttgg
       61 taagtcggat gtgaaaacca gaggctcaac ctctggcctg catccgatac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000387
VERSION     KHUT01000387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           92 g           41 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtccgc
       61 aaagtctgat gtgaaagccc cgggctcaac ctgggaactg cattggaaac taacgggctc
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000388
VERSION     KHUT01000388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggttgg
       61 taagtccgtg gtgaaatctc caagcttaac ttggaaactg ccgtggatac tatcaatctt
      121 gaatatagtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctcgctaca ctattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000389
VERSION     KHUT01000389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtttg
       61 taagtcagtg gtgaaatccc gaagcttaac ttcggaactg ccgttgatac tacaggtctt
      121 gaatactcgg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaca gagtcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000390
VERSION     KHUT01000390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           80 g           54 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggttcga
       61 aaagtccact gttaaaaatt gaggcttaac ttcaaagatg ccgtggaaac tttcaaacta
      121 gagtatggta ggggtagagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaccaccgat ggcgaaggca ctctactggg ccattactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01000391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000391
VERSION     KHUT01000391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           36 c           78 g           65 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggatgt
       61 taagtcaggg gtgaaagttt gcagctcaac tgtaaaattg cctttgatac tggcgttctt
      121 gaattattgt gaagtggtta gaatgtgtag tgtagcggtg aaatgcatag atattacaca
      181 gaataccgat tgcgaaggca gatcactaac aatgaattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000392
VERSION     KHUT01000392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           52 c           86 g           44 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccaag
       61 tgtgtgaggc gtgaaagccc gatgctcaac atcggaattg cacctcaaac tacatggcta
      121 gagcattgga gagggtagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000393
VERSION     KHUT01000393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 caagacaggt gtgaaatccc cgggcttaac ctgggaactg cacttgtgac tgcacggctg
      121 gagtacggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca gtcccctggg cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000394
VERSION     KHUT01000394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagttcgtt gtgaaagccc cgggctcaac ctgggaatgg caatggaaac tggcgggctt
      121 gagtatggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000395
VERSION     KHUT01000395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggct gtgaaaacct ggggctcaac tccgggcctg cagccgatac gggcaagcta
      121 gaattcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgaaattga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000396             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000396
VERSION     KHUT01000396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           93 g           41 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcgggt gtgaaacccc caggctcaac ctggggacgc cacccgatac tgctgtggct
      121 agagtccagt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccaa cggcgaaggc agcactctgg gctggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000397
VERSION     KHUT01000397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgatt
       61 caagtcagag gtgaaagccc ggggctcaac cccggaactg cctttgaaac tagattgcta
      121 gaatcctgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactgga caggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000398
VERSION     KHUT01000398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaatctc agagctcaac tctgacattg cagtcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000399
VERSION     KHUT01000399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgcagggctt
      121 gagtacagat gaagtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000400
VERSION     KHUT01000400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggccgac
       61 taagtcagac gtgaaatccc tcagctcaac tggggaactg cgtctgagac tggccggctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgctactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000401
VERSION     KHUT01000401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           90 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggttata
       61 caagtctgct gtcaaagcgc aaggctcaac cttgtaaagg cagtggaaac tgtgtgacta
      121 gagagtgata ggggcagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctgctggg tcatatctga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000402
VERSION     KHUT01000402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcagtg
       61 caagtcaggt gtgaaatccc ggggctcaac cccggaactg cacttgatac tgctctgcta
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000403
VERSION     KHUT01000403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgagac tgtctagcta
      121 gagtatggga gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000404
VERSION     KHUT01000404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggtttgt
       61 taagtgagag gtgaaatccc agagctcaac tctggaactg cctttcatac tggcaagcta
      121 gagtccggaa gaggtaagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000405
VERSION     KHUT01000405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 tgtgtctgct gtgaaatccc cgggctcaac ctgggaatgg cagtggaaac tggcgagctt
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca accccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000406
VERSION     KHUT01000406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           75 g           64 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggccttt
       61 taagtcagtg gtgaaatctt gccgcttaac ggtaaaattg ccattgatac tgaaaggctt
      121 gagtacattt gaggtaagcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gcttactaaa ctgttactga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000407
VERSION     KHUT01000407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           85 g           56 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctttg
       61 taagttagag gtgaaagccc agagctcaac tctggaattg cctttaagac tgcatcgctt
      121 gaatcgtgga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactgga cacgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000408
VERSION     KHUT01000408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agggtgcgta ggtggttgtt
       61 taagtctgtt gtgaaagccc cgggctcaac ctgggaatgg caatggatac tggacagcta
      121 gagtgcggta gagggtagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccagcactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000409
VERSION     KHUT01000409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggacaag
       61 taagtcagtg gtgaaatctc tgggcttaac tcagaaactg ccgttgatac tatttgtctt
      121 gaatatcatg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttactaca tggagattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000410
VERSION     KHUT01000410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 cacgtaggat ccgagcgtta tccgaattta ctgggcgtaa agcgcgtgta ggcggttcgg
       61 taagttggac gtgaaatctc ccggctcaac tgggagaggt cgtacaaaac tgccgggcta
      121 gagggcggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccgcacctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000411             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000411
VERSION     KHUT01000411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggccaat
       61 caagtcagat gtgaaagccc acggcttaac cgtgtgaagt gcatctgaaa ctggtaggct
      121 tgagtatcgg agaggaaagt ggaattcccg gtgtagaggt gaaattcgta gatatcggga
      181 ggaataccgg tggcgaaggc gactttctgg acgaatactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000412
VERSION     KHUT01000412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           85 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttga
       61 taagttgaat gtgaaagccc cgggctcaac ctgggaattg catccaaaac tgtctgacta
      121 gagtacggta gagggtagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000413
VERSION     KHUT01000413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           46 c           98 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggtttgt
       61 tgagttgggc gtgaaattcc tgggcttaac ctgggggctg cgttcaatac cagcaggctt
      121 gagtgtggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000414
VERSION     KHUT01000414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttg
       61 taagtcggat gtgaaatccc gcggctcaac cgtggaagtg cactcgaaac tgcaaggcta
      121 gagtatcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgattactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000415
VERSION     KHUT01000415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcggct gtgaaaaccc atggctcaac cgtgggcttg cagccgatac gggcaggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000416
VERSION     KHUT01000416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           79 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccgttgatac tatttgtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cggtcattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000417
VERSION     KHUT01000417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatttt
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tggagatctt
      121 gagtccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000418
VERSION     KHUT01000418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          106 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgtcgagctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000419
VERSION     KHUT01000419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           79 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggactgg
       61 taagtccgtg gtgaaatccc caagcttaac ttgggaactg ccgtggatac tattagtctt
      121 gaatactgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttgctaca cagtcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000420
VERSION     KHUT01000420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcgggcttt
       61 taagccagac gtgaaatccc cgggcttaac ctgggaatgg cgtttgggac tggaagtctg
      121 gagtgcggca gaggagactg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000421
VERSION     KHUT01000421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctcga
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgatac tgtagagctt
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000422
VERSION     KHUT01000422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           98 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttgga
       61 taagtcagct gtgaaaacca cgggcttaac tcgtggcctg cagttgagac tgtctggcta
      121 gagtgtggga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000423
VERSION     KHUT01000423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgg
       61 tcagtcccgt gtgaaatccc tcggctcaac tgaggaactg cacgggaaac tgcctggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000424
VERSION     KHUT01000424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           90 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 taagtcaggt gtgaaatttc ggagcttaac tccgaaactg catttgatac tgccttgctt
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000425
VERSION     KHUT01000425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           87 g           51 t
ORIGIN      
        1 tacgaaccgt acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccatg
       61 taagtggggt gtgaaatccc tcggctcaac cgaggaactg cgctccatac tgcatggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01000426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000426
VERSION     KHUT01000426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 taagtcaggt gtgaaatctc ggggctcaac cccgaaactg cacttgatac tgccttgctc
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000427
VERSION     KHUT01000427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcgcc
       61 taagtcggac gtgaaatccc caagcttaac ttgggaactg cgtccgatac tgggtgactt
      121 gagttcggga gaggaatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccgaaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000428
VERSION     KHUT01000428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggctggt
       61 caagtccgat gtgaaagctc ccggcttaac tgggaaattg catcggaaac tgatcggctg
      121 gaaggtggga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctacctggc ccattcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000429
VERSION     KHUT01000429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggttgat
       61 taagtcagat gtgaaatccc cgagcttaac ttgggaactg catttgaaac tggtcagcta
      121 gagtcttgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccggt ggcgaaggcg gccccctgga caaagactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000430
VERSION     KHUT01000430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           85 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaatctc acagcttaac tgtgaaactg cattggatac tatccggctt
      121 gaggaatgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000431
VERSION     KHUT01000431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccat
       61 cacgtcggga gtgaaaagct ggggcttaac cccagtcctg cttccgatac gggtggacta
      121 gaggtaggca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000432
VERSION     KHUT01000432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatctt
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tggggatctc
      121 gagtccggaa gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000433             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000433
VERSION     KHUT01000433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcggat gtgaaaactc caggctcaac ctggagacgc cattcgatac tgctgtggct
      121 tgagtctggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000434
VERSION     KHUT01000434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtggatg gtgaaatccc tcggcttaac cgaggaactg ccgtccaaac tgcttcgctt
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000435
VERSION     KHUT01000435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           88 g           50 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgctg
       61 taagtcgggt gtgaaatccc acagctcaac tgtggaactg cgcccgatac tgcagtgcta
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01000436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000436
VERSION     KHUT01000436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tataggtctt
      121 gaattatctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg gatgtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000437
VERSION     KHUT01000437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgca ggcggccttg
       61 caagtcggac gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcgaggctg
      121 gagtcctgga gaggaaagtg gaatactcgg tgtagaggtg aaattcgtag atatcgagtg
      181 gaacaccggt ggcgaaggcg gctttctgga caggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000438             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000438
VERSION     KHUT01000438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcttgt
       61 taagtctaag gtgaaaggca gtggctcaac cacttgcagg gccctggata ctggcaggct
      121 cgagtgcgcg tgaggccggt ggaacgggtg gtgtagcggt gaaatgcata gataccatcc
      181 agaacaccga ttgcggaggc agccggctaa cgcgcaactg acactgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000439
VERSION     KHUT01000439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           43 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctact
       61 taagtcggat gtgaaagccc ctggctcaac tggggaagtg cacccgaaac tgagtggctt
      121 gagtgcgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000440
VERSION     KHUT01000440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcatgg
       61 taagtttgat gtgaaagctc agggctcaac cctgaaatgg cattgaatac tactgtgctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000441             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000441
VERSION     KHUT01000441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           53 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcatgt
       61 taagtcagct gtgaaatctc cgagcttaac tcggaactgt cagctgatac tgatgtgctt
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgagtgc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000442
VERSION     KHUT01000442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           92 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttttc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tggaaggctt
      121 gagtatggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttactgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000443
VERSION     KHUT01000443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttctg
       61 caagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tacggagcta
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000444
VERSION     KHUT01000444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           93 g           54 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtctgtt gtgaaagccc tgggctcaac ccgggaagtg cactggatac tggcagactt
      121 gaatacggga gaggagagtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000445
VERSION     KHUT01000445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agggtgcgta ggtggtcgtt
       61 taagtccgtt gtgaaatccc tgggctcaac ctgggaatgg cagtggatac tggacggcta
      121 gaatgcggta gagggtagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccagcattga cactcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000446
VERSION     KHUT01000446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcttc
       61 taagtcagtt gtgaaatact tcagcttaac tggagaggtg caattgatac tggaagactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000447
VERSION     KHUT01000447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacgtaggtc acaagcgttg tccggattta ttgggcgtaa agggagcgca ggcggtctgt
       61 taagtctgat gtgaaagccc acggctcaac cgtggaactg cattggaaac tggtagactt
      121 gagtgcagaa gaggaaagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000448
VERSION     KHUT01000448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtttgt
       61 taagtcatgt gtgaaatccc ccggctcaac cggggaacga cgcatgaaac tggcaagcta
      121 gagtactgaa gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccccctggt cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000449             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000449
VERSION     KHUT01000449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           42 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggttcag
       61 caagtcaact gtgaaaaccc ggggctcaac cccgggacgc cagtcgaaac tgctgtgact
      121 agggtccggt agaggtgagt ggaattctcg gtgtagcggt gaaatgcgca gatatcgaga
      181 ggaacaccag tagcgaaggc ggctcactgg gccggtaccg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000450             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000450
VERSION     KHUT01000450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           48 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 tgagtcgggt gtgaaatctc cacgctcaac gtggaggggc cacccgatac tgccatgact
      121 agagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc gacactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000451
VERSION     KHUT01000451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccatt
       61 caagtcaggg gtgaaagcct gtggctcaac cacagaattg cctttgatac tgtttggcta
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000452
VERSION     KHUT01000452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggctgaa
       61 taagtgggat gttaaatccc ccggctcaac cggggacctg catctcatac tgttcagctc
      121 gagcacatca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg atgatgctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000453
VERSION     KHUT01000453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           74 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtttg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tatagatctt
      121 gaattatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg gatatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000454
VERSION     KHUT01000454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgag
       61 taagtcgaat gtgaaagccc ccggcttaac tggggagggt cattcgatac tgttcgactt
      121 gaaggcagga gagggcagca gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctgcctggc ctgttcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000455             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000455
VERSION     KHUT01000455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctaag
       61 taagtcggcg gtgaaaagcc aaggctcaac cttggtactg ccgtcgatac tgctttggct
      121 agagtccggt agaggagaac ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacacctg tagcgaaggc ggttctctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000456
VERSION     KHUT01000456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggtgag
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccgttgatac tacttgtctt
      121 gaatatcgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgctaca cgaatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000457
VERSION     KHUT01000457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagttagag gtgaaagcct ggggctcaac tccagaattg cctttaagac tgcatcgctt
      121 gaatcatgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcgctgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000458
VERSION     KHUT01000458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacagagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccatg
       61 cgtgtcttcc gtgaaatccc tcggcttaac tgaggacgtg cggaagaaac tgcaaggctc
      121 gagtccggga gaggggggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccccctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000459
VERSION     KHUT01000459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagag gtgaaagacg gttgctcaac aatcgcagtg cctttgatac tggagagctt
      121 gagtacagtt gaggtaggcg gaatgcaggg tgtagcggtg aaatgcatag ataccctgca
      181 gaacaccgat tgcgaaggca gcttactaag ttgttactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000460
VERSION     KHUT01000460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           46 c           98 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctatc gtgaaaactg gaggcttaac tttcagcgtg cggtggatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000461
VERSION     KHUT01000461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c          102 g           45 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtac agggagcgta ggcggttcgg
       61 taagccctct gtgaaatctc caggcttaac ctggaacagt cggaggggac tgctgggcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtttctga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000462
VERSION     KHUT01000462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacgaggggg gcaagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcggatgtt
       61 taagttggat gtttaaaacc ggggcctaac ctcggagcgg catccaaaac tgggcatctt
      121 gaataggtca gaggctggtg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacacctat tgcgaaggca gccagctggg accatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01000463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000463
VERSION     KHUT01000463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggttctt
       61 caagtcagag gtgaaagccc agagctcaac tctggaatgg cctttgagac tagagaacta
      121 gggtacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgttaccga cgctcaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000464
VERSION     KHUT01000464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggaccga
       61 taagtcagtg gtgaaatctc atagcttaac tatgaaactg ccattgatac tgtcggtctt
      121 gagtaaattt gaagtggctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggtcactaag atttaactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000465
VERSION     KHUT01000465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c          100 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 tgcgtcaacc gtgaaatcct gctgctcaac ggcaggtctg cggttgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000466
VERSION     KHUT01000466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           89 g           50 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggaagtt
       61 caagtcagct gttaaagcgt gaggcttaac ctcataaggg cagttgaaac tgagcatcta
      121 gagtgcgata ggggcaaggg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg ccttgctggg tctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000467
VERSION     KHUT01000467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 taagtcgatt gtgaaatccc cgggctcaac ctgggaattg cagtcgagac tgtcagacta
      121 gagtgtggga gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accgcctggc ccaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000468
VERSION     KHUT01000468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtggtt
       61 taagtctgct gtgaaagccc tgggctcaac ctgggaattg cagtggatac tggattacta
      121 gagtgtggta gagggatgcg gaatttctgg tgtagcagtg aaatgcgtag agatcagaag
      181 gaacatccgt ggcgaaggcg gcatcctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000469
VERSION     KHUT01000469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           96 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtctgt
       61 tgcgtcagga gtgaaaactc ggggcttaac cccgagcctg cttctgatac gggcagacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000470
VERSION     KHUT01000470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggtggcctcg
       61 caagtcgagt gtgaaatccc cgggcttaac ttgggaattg cgctcgaaac tacggggctt
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gcctcctggg ccaacactga cactcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000471
VERSION     KHUT01000471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggttctg
       61 tatgtctacc gtgaaagcat acagctcaac tgtattttgt cggtagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000472
VERSION     KHUT01000472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           98 g           46 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 tacgtctgct gtgaaaaccc ggggcttaac cctgggcttg cagtggatac gggcagacta
      121 gaggcaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000473             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000473
VERSION     KHUT01000473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtctggt gtgaaagctc cgagctcaac tcggaaaagc attggaaact atcccgcttg
      121 agggtcggag gggagactgg aattctcggt gtagcagtga aatgcgtaga tatcgagagg
      181 aacaccagtg gcgaaggcga gtctctggac gactcctgac actgaggcac gaaagctagg
      241 ggagcaaaca gg
//
LOCUS       KHUT01000474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000474
VERSION     KHUT01000474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           94 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttttc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tgggagacta
      121 gagtgcggga tgggagagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggtgtagacg gctctctgga ccgccactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000475
VERSION     KHUT01000475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtacgta ggtggtttgt
       61 taagccagct gtgaaatccc cgggctcaac ctgggcactg cagttggaac tggcaagcta
      121 gagtagggta gaggggtgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg acaccctgga ctcatactga cactgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000476
VERSION     KHUT01000476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttgt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tggcaggcta
      121 gagtatggta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg acttcctgga ccaatactga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000477
VERSION     KHUT01000477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           82 g           63 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggttggaatg
       61 taagtcagtg gtgaaatact gccgcttaac ggtagagctg ccattgatac tgcgtttctt
      121 gagtatggtt gaggtaggtg gaatgtgttg tgtagcggtg aaatgcatag atatgacaca
      181 gaacgccgat tgcgaaggca acttactaag ccattactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000478
VERSION     KHUT01000478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggctcga
       61 taagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaagac tgtcgggctt
      121 gaggatggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctgga ccacacctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000479
VERSION     KHUT01000479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgaat
       61 caagtcagct gtgaaatctc cgagcttaac tcggaacggt cagctgatac tgttttgcta
      121 gagtgcagaa agggcaaccg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaagcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000480
VERSION     KHUT01000480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          102 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggccgag
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tactcagctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000481
VERSION     KHUT01000481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          102 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccacc
       61 caagtcgatc gtgaaagctc ggggctcaac cccgagattg cggtcgatac tgagtggcta
      121 gaggctggta ggggggtgtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gcaccctggg ccagtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000482
VERSION     KHUT01000482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggctggg
       61 caagtccgat gtgaaagctt ccggcttaac tggaaaattg catcggaaac tgctcagctt
      121 gaaggtggga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggc ccattcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000483
VERSION     KHUT01000483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcccac
       61 taagtctgat gtgaaagccc ggggctcacc cccggaagtg cattggaaac tggtggactc
      121 gagtcccgga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000484             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000484
VERSION     KHUT01000484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtctggt gtgaaatctc cacgctcaac gtggaggggc cacccgatac tgccatgact
      121 ggagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000485
VERSION     KHUT01000485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           95 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggttttt
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaagtg cattggaaac tgggagactt
      121 gaatacggga gagggcagtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000486
VERSION     KHUT01000486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgct gtgaaatccc cgggctcaac ctgggaatgg cagtggatac tggcaagcta
      121 gagtgcggta gagggatgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctgga ccagcactga cactcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000487
VERSION     KHUT01000487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           89 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggtagg
       61 caagtcgggc gtgaaatccc tgagctcaac tcaggaactg cactcgaaac tgcttatctt
      121 gagtaccgga gaggaaagcg gaatacctgg tgtagaggtg aaattcgtag atatcaggtg
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000488
VERSION     KHUT01000488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtccgac gtgaaatccc aaagcttaac tttggaactg cgtcggatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgacattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000489
VERSION     KHUT01000489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcactg
       61 ttagtcaggc gtgaaagtcc tgggctcaac ctgggaactg cgcttgatac ggcagagcta
      121 gaggatggaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000490
VERSION     KHUT01000490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgat
       61 taagtctggg gtgaaagccc gttgctcaac aacggaactg ccctggaaac tggtcggctt
      121 gagtacagac gagggtggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000491
VERSION     KHUT01000491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           97 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgct gtaaaagtca ggggctcaac ccttgaacgc cggtggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000492
VERSION     KHUT01000492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 taagttgggt gtgaaatccc agggctcaac cctggaagtg cattcaaaac tgctcggcta
      121 gagttctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagaaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000493
VERSION     KHUT01000493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           84 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctta
       61 taagtcagtg ctgaaatatc ccggctcaac cgggagggtg gcattgatac tgtagggctt
      121 gagtaaagtt gaggttggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gctaactaag ctttaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000494
VERSION     KHUT01000494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttatt
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgggtaacta
      121 gagtttaata gaggagagtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacaccagt ggcgaaggcg actctctgga ttaaaactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000495
VERSION     KHUT01000495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtga
       61 caagtcactt gtgaaatctc cgggcttaac tcggaacggt caagtgatac tgtcgtgcta
      121 gagtgcagaa agggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaggcg agttgctagg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000496
VERSION     KHUT01000496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcaggt
       61 taagtaaggt gtgaaatctc cctgctcaac ggggagggtg cactttagac tgaccagcta
      121 gagggcggta gagggtggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccacctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000497
VERSION     KHUT01000497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           94 g           50 t
ORIGIN      
        1 tacgtagggc gcaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgcc gtgaaagacc agggctcaac cctggttctg cggtgggtac gggcagacta
      121 gagtgatgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg catctactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000498
VERSION     KHUT01000498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacggggggt gcaaacgttg ttcggaatca ctgggcgtaa agagcgcgta ggcggccttg
       61 taagtggggt gtgaaatccc ccggctcaac cggggaactg catcccatac tgcttggctt
      121 gagggcagca gaggaaacgg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg cgtttctggg ctgcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000499
VERSION     KHUT01000499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcgggttct
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cccttgatac tgagaatctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000500
VERSION     KHUT01000500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 tgtgtccggt gtgaaagccc cgagcttaac ttgggaatgg cattggaaac tggcgagctg
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000501
VERSION     KHUT01000501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 caggtccgtt gtgaaaactg gaggctcaac ctccagacgt cgatggaaac cgcttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000502             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000502
VERSION     KHUT01000502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           42 c           94 g           57 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttgtt
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaatac tgaatgactt
      121 ggagtgcggg agaggaaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggtgtagac ggctttctgg accgtaactg acgctgagac acgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000503
VERSION     KHUT01000503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggatagt
       61 ttagtcagag gtgaaagccc agggctcaac cttggaattg cctttgatac tggctatctt
      121 gagtacggaa gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggt ccgttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000504
VERSION     KHUT01000504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           90 g           58 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtgtgg
       61 taagtcaagt gtgaaatctc ggagctcaac tttgaaactg cacttgatac tgccgtgcta
      121 gagggttgta gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg gatctctggg caattcctga cgctgaggca cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000505
VERSION     KHUT01000505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           96 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgct gtgaaagccc tgggctcaac ctgggaatgg cggtggatac tgacaagcta
      121 gagtgcggta gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccctctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000506             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000506
VERSION     KHUT01000506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           47 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcaggt gtgaaatctc cacgctcaac gtggaggcgc cacctgaaac tgccgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000507
VERSION     KHUT01000507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggccaga
       61 taagtccggt gtgaaagtca caggctcaac ctgtgaatgc cgctggaaac tgtctggcta
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000508
VERSION     KHUT01000508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggcgttt
       61 taagtcgggg gtgaaagcct gtggctcaac cacagaatgg ccttcgatac tgggacgctt
      121 gagtatggta gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000509
VERSION     KHUT01000509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           82 g           56 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cccttgatac tgatgggctt
      121 gattacagtt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactaaa gtgtaaatga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000510
VERSION     KHUT01000510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggccact
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tgagtggctt
      121 gagtgcagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctgtaactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000511
VERSION     KHUT01000511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           94 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatga ctgggcgtaa agcgcgtgta ggcggcctcg
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgcaaggctt
      121 gagtcccgga gaggaaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctttctgga cggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000512
VERSION     KHUT01000512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          103 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 atagtcggcg gtgaaagccc ggagctcaac tccgggtcgg ccgtcgatac ttccgggctt
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000513
VERSION     KHUT01000513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          107 g           39 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggagtta ctgggcgtaa agggtgcgca ggcggttggt
       61 tacgtcgtgt gtgaaagccc cccgcttaac gggggagggt cgcgcgaaac gggcgaactg
      121 gaggcaaggc gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gccgactgga cttgacctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000514
VERSION     KHUT01000514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cacgtcggct gtgaaaaccc gaggctcaac ctcgggcctg cagtcgatac gggcaaacta
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000515
VERSION     KHUT01000515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgat
       61 caagtcggag gtgaaatccc tcggctcaac tgaggaattg ccttcgatac tgttcggctt
      121 gagtcctgga gagggtagcg gaattcccgg tgtagcggtg aaatgcgtag agaccgggaa
      181 gaacaccagt ggcgaaggcg gctacctgga caggtactga cgctgaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000516
VERSION     KHUT01000516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggttct
       61 taagttaggt gtttaatccc ggggcccaac cccggaccgg cacttaaaac tgggaatctt
      121 gaatagtgga gaggtcggtg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccagt tgcgaaggcg gccgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01000517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000517
VERSION     KHUT01000517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           82 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggacttt
       61 taagtcagcg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tattggtctt
      121 gaatgttgtt gaggtgggcg gaacgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgtaggca gcccactaag caacgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000518
VERSION     KHUT01000518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           96 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtttgt
       61 cgtgtctggt gtgaaatccc cgggctcaac ctgggaatgg cattggaaac tggcagacta
      121 gagtgtggca gagggaggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gcctcctggg ctaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000519
VERSION     KHUT01000519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           84 g           51 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttgt
       61 tcagtctcct gtgaaatatc agggcttaac cctgagccag cagtggaaac taacaagcta
      121 gaggacggta ggggcagagg gaattcccgt tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccaat ggcgaaagca ctctgctggg ccgtatctga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01000520             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000520
VERSION     KHUT01000520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           96 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgg
       61 taagtcggat gtgaaatctc caggctcaac ctggaggggt cattcgatac tgccgtgact
      121 agaggtcggt aggggagtgt ggaattcctg gtggagcggt gaaatgcgca gatatcagga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgatactg acactgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000521
VERSION     KHUT01000521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccgat
       61 caagtcgggg gtgaaagcct gtggctcaac cacagaatgg ccttcgatac tgttcggctc
      121 gagaccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000522
VERSION     KHUT01000522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgaagggg actagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggatcac
       61 taagtcaggg gtgaaatccc gaggcttaac ctcggaactg cctttgatac tggtgatctg
      121 gagatcggga gaggttggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gccaactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000523
VERSION     KHUT01000523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtcttg
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgcaaggctg
      121 gagtatggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000524
VERSION     KHUT01000524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaatgg cattggaaac tggcaagcta
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000525
VERSION     KHUT01000525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           87 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cattagaaac tggaatgcta
      121 gaggatggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccatttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000526
VERSION     KHUT01000526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctggt gtgaaaactc aaggctcaac cttgagcttg catcgggtac gggcagacta
      121 gagtgcggta ggggtgactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtcactggg ccgcaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000527
VERSION     KHUT01000527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           85 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatact ccagcttaac tggaggggtg ccattgatac tgcagggctt
      121 gagtggagtc gaagtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg cttctactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000528
VERSION     KHUT01000528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagccagat gtgaaatccc cgggctcaac ctgggaatgg catttgggac tgcaaggctg
      121 gagtttggca gaggagactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaataccgat ggcgaaggca ggtctctggg ctataactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000529
VERSION     KHUT01000529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           89 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgttc
       61 ccagtctgtt gtgaaatctc ccggcttaac tgggagggtg cagcggaaac tagaatgcta
      121 gagggtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccacttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000530
VERSION     KHUT01000530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgct
       61 caagtccggg gtgaaatcct ccagctcaac tggagaactg cctcggatac tgggtgcctc
      121 gagggtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccacttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000531
VERSION     KHUT01000531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggagtcta ggcggtttgg
       61 taagtgggat gtgaaatccc cgggctcacc ccgggacctg catcccaaac tgctgagctt
      121 gagtacagga gagggtgggg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg cccacctggc ctgctactga cgctgaagct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000532             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000532
VERSION     KHUT01000532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgtta cccggattta ctgggcgtaa agcgcgtgta ggcggctggt
       61 caagcgtggt gtgaaatctc cgggctcaac ccggaaactg cattgcgaac tgacctggct
      121 ggagtgcagg agagggcagc ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 agaacaccag tggcgaaggc ggctgcctgg cctgtaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01000533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000533
VERSION     KHUT01000533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           84 g           51 t
ORIGIN      
        1 gacgagccac ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 caagtcggga gtgaaatacc tcagcttaac tgaggaactg ctttcgatac tgctgtgctt
      121 gagtaagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gcttactggg tcttaactga cgctgaggca cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000534
VERSION     KHUT01000534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggcgccg
       61 caagtcgagt gtgaaagccc cgggcttaac ttgggaattg cgctcgaaac tatggtgctg
      121 gagtgtggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccttctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000535
VERSION     KHUT01000535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatttg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccatggatac tataagtctt
      121 gaatgttgtg gaggtttgcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcaggctaca caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000536
VERSION     KHUT01000536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcttgg
       61 cgagtgaggc gtgaaaggcc tgggctcaac ccgggaacgg cgcttcagac tgccaggctt
      121 gagttcggga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggc ccgcaactga cgctacagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000537
VERSION     KHUT01000537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           75 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtctta
       61 taagtcagtg gtgaaatctc cccgctcaac ggggaaacgg ccattgatac tgtaggactt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000538
VERSION     KHUT01000538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggctgtc
       61 tacgtccggg gtgaaagccc agagctcaac tctgggactg ccttggatac gggatggctt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000539
VERSION     KHUT01000539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           78 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtttat
       61 taagtcagtg gtgaaagccc ggagctcaac ttcggaactg ccattgatac tgatagactt
      121 gagtacagat gaagtgggcg gaatgtgaca agtagcggtg aaatgcttag atatgtcaca
      181 gaacaccgat tgcgaaggca gctcactaaa gtgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000540
VERSION     KHUT01000540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cacgtcggac gtgaaaatcc agggctcaac cctggacctg cgttcgatac gggcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgaaactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000541
VERSION     KHUT01000541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttga
       61 taagtcggct gtgaaagtcg ggggctcaac cctcgaatgc cggccgatac tgtcaagcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000542
VERSION     KHUT01000542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           71 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggataa
       61 tcagtcagtg gtgaaagtct gcagctcaac tgtagaattg ccattgatac tgttatcctt
      121 gagttattat gaagtggtta gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaataccgat tgcgaaggca gatcactaat aatacactga cgctgatgga cgaaagcgta
      241 ggtagcgaac agg
//
LOCUS       KHUT01000543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000543
VERSION     KHUT01000543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c           99 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcagct gtgaaaaccc gcagctcaac tgtgggcttg cagctgatac gggcaggctt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000544
VERSION     KHUT01000544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          103 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gcagtcaacg gtgaaagctt ggggctcaac cccgagtcgg ccgtcgatac tcccgtgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000545
VERSION     KHUT01000545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttcgt
       61 taagtcctgt gtgaaatccc ccggctcaac tggggaactg cacaggaaac tagcgggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000546
VERSION     KHUT01000546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           89 g           56 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttgg
       61 taagtcttat gtgaaatcta caggctcaac ttgtagtctg catgagaaac tgccgggctt
      121 gagtatggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000547
VERSION     KHUT01000547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           84 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggttag
       61 taagtcagtg gtgaaagccg gcagctcaac tgtcgaattg ccattgatac tgttaacctt
      121 gagtatagtt gaggtaggcg gaatggaagg tgtagcggtg aaatgcatag atatcttcca
      181 gaacaccgat agcgtaggca gcttactaag ctataactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000548
VERSION     KHUT01000548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccatt
       61 caagtgacgg gtgaaagccc tcggcttaac cgaggaactg cctgtcagac tggatggctc
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000549
VERSION     KHUT01000549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           81 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtccgtg gtgaaatctc tgagcttaac tcggaaactg ccatggatac tatccgtctt
      121 gaatgcggtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgctaca cctgcattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000550
VERSION     KHUT01000550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           95 g           45 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggtttgg
       61 caagtcggat gtgaaatccc gaggctcaac ctcgggactg catccgatac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000551
VERSION     KHUT01000551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           80 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 gaagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tctctgtctt
      121 gaatcccgtg gagatgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgttaca cgaagattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000552
VERSION     KHUT01000552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttagg
       61 taagttggat gtgaaagccc tgggctcaac ctgggaactg catccaaaac tgcttggcta
      121 gagtacggta gagggtagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000553
VERSION     KHUT01000553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           98 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagtagggg gtgaaatctt gcggctcaac cgcaagactg ccttctaaac tgcctggctt
      121 gagcacagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ctgttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000554
VERSION     KHUT01000554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagttgggc gtgaaagctc ccggctcaac tgggagaggt cgtccaaaac taccagacta
      121 gagagcgata gaggaaaatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg attttctgga tcgtttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000555             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000555
VERSION     KHUT01000555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          105 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggctctt
       61 taagtctctt ggtgaaaggc cggggctcaa ccctgagcat gccggggata ctggggagct
      121 ggagacaggc agaggccagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggctggctgg gcctgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000556
VERSION     KHUT01000556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttt
       61 taagccagat gtgaaatccc cgggctcaac ctgggaactg catttggaac tggaagacta
      121 gagtgtagaa gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000557
VERSION     KHUT01000557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c           96 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtttgt
       61 ttagtctagg gtgaaagctc ccggcttaac tgggagaggt ccttggatac tggcaaactt
      121 gagggaggca gaggaaagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccttacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01000558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000558
VERSION     KHUT01000558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           83 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggtggtatcg
       61 taagtcagaa gtgaaagttt gcggcttaac cgtaaaattg cttttgatac tgcaatactt
      121 gaatcaggtt gaggttggcg gaatgtggca tgtagcggtg aaatgcttag atatgccata
      181 gaacaccgat tgcgaaggca gctgactgga cctgtattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000559
VERSION     KHUT01000559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           82 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggatcaa
       61 taagtcagtg gtgaaagccg gcagctcaac tgtcgaattg ccattgatac tgttgatctt
      121 gagtatagtt gaggtaggcg gaatgagtcg tgtagcggtg aaatgcatag atatgactca
      181 gaacaccgat agcgtaggca gcttactaag ctataactga cgctgaggca cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000560
VERSION     KHUT01000560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 cacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtttgta ggcggccttg
       61 taagtcaggt gtgaaagccc tcggctcaac cgaggaacag cgcttgatac tgcaaggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01000561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000561
VERSION     KHUT01000561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           96 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcttgta ggcggttcgt
       61 taagtccggt gtgaaatctc ccggctcaac tgggagcggc catcggatac tggcgagcta
      121 gaggtgggca gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggg cctatcctga cgctgaggag cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000562
VERSION     KHUT01000562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtgcgg
       61 taagtcttct gtgaaatctc cgggctcaac tcggagcctg caggagaaac tgccgtgctt
      121 gagtatggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000563
VERSION     KHUT01000563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           80 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggctgttttg
       61 taagtcagat gtgaaatccc tgagctcaac ttaggaactg cgtttgaaac tacaagacta
      121 gaatatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccaat ggcgaaggca gccccctggg ataatattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000564
VERSION     KHUT01000564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           95 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttgc
       61 taagtcagat gtgaaagtcc acggctcaac cgtggaagtg catttgaaac tggcaggctt
      121 gagtactgga gggggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg accacctggc cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000565
VERSION     KHUT01000565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c          107 g           41 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgtga
       61 taagggtgtg gtgaaagccc ggggctcaac cccgggtctg ccatgccgac tgtcaggcta
      121 gagtactgta ggggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggag
      181 gaagaccggt ggcgaaggcg gcctgctggg cagttactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000566             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000566
VERSION     KHUT01000566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggtctcg
       61 taagctgggt gtgaaagccc cgggcttaac ctgggaatgg cattcaggac tgcgaggctt
      121 tgagtgtggc agagggaggt ggaattccac gtgtagcagt gaaatgcgta gagatgtgga
      181 ggaacaccga tggcgaaggc agcctcctgg gccagcactg acgctcatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000567
VERSION     KHUT01000567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           79 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggccatt
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cctttgaaac tgaatggctt
      121 gaatgtactt gaggtgggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaaccccgat tgcgaaggca gctcactaaa gtatgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000568
VERSION     KHUT01000568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c          102 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggtccga
       61 cacgttttgt gtgacagcgg gtggcttaac tacccgagga tgcaaaagac ggttggactt
      121 gaggactcga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc gagtgactga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000569
VERSION     KHUT01000569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           48 c           98 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtcggcc gtgaaaactt cacgcttaac gtggagcttg cggtcgatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000570
VERSION     KHUT01000570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           87 g           53 t
ORIGIN      
        1 tacgtaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtttgta ggcggcgctg
       61 ttagtcgggt gtgaaatccc agggctcaac cctggaactg cgcccgatac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgagaaa cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01000571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000571
VERSION     KHUT01000571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggccagta ggcggccaac
       61 taagtccgac gtgaaatccc aaggcttaac cttggaactg cgtcggatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgacattga cgctgaacgg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000572
VERSION     KHUT01000572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccgttgatac tatctgtctt
      121 gaatactatg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca cagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000573
VERSION     KHUT01000573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatagctctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca caaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000574
VERSION     KHUT01000574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagttcgta ggcggtttgt
       61 cgcgtcgtgt gtgaaatccc gcagctcaac tgcgggcttg caggcgatac gggcaaactt
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggaa cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000575             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000575
VERSION     KHUT01000575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcggct gtgaaatccc gaggcttaac ctcggaactg cagtcgatac tgctgtggct
      121 agagtctggt agaggagaat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000576
VERSION     KHUT01000576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           82 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcagtg gtgaaatatc tcagcttaac tgagaaggtg ccaatgatac tgatgggctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000577
VERSION     KHUT01000577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 gacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggcggctttt
       61 taagtctgtt gtcaaagccc acagctcaac tgtggatcgg caatggaaac tgggaagcta
      121 gagtgtggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg ctctactggg ccacaactga cgctgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000578
VERSION     KHUT01000578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctcat
       61 tttgtcaggc gtgaaagccc cgggcttaac ctgggaactg cgtttgatac ggatgggcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000579
VERSION     KHUT01000579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcctgg
       61 aaggtccgtt gtgaaaactc gaggctcaac ctcgagacgt cgatggaaac cctcaggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000580             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000580
VERSION     KHUT01000580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           94 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cgcgtcgtct gtgaaattcc ggggcttaac tccgggcgtg caggcgatac gggcataact
      121 tgagtgctgt aggggagact ggaattcctg gtgtagcggt gatatgcgca gatatcagga
      181 ggaacaccaa tggcgaaggc aggtctctgg gcagttactg acgctgagga gcgaaagcat
      241 gggtagcgaa cagg
//
LOCUS       KHUT01000581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000581
VERSION     KHUT01000581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          104 g           43 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggatgg
       61 caagtcggtc gtgaaatccc tgggctcaac tcaggggggt cggccgatac tgccgttctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cattccctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000582
VERSION     KHUT01000582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggtggttcct
       61 taagtctgat gtgaaagccc acggctcaac cgtggaaggt cattggaaac tggggaactt
      121 gaatgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgttattga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000583
VERSION     KHUT01000583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcccat
       61 caagtcggat gtgaaatctc aaggctcaac cttgaaattg catccgatac tgctgggctt
      121 gaggcaggta ggggaaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000584             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000584
VERSION     KHUT01000584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          102 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtcttt
       61 taagtcttgc cgtgaaatgc cggggctcaa ccccggccct gcgggggata ctgggagact
      121 ggagacaagt agaggcgagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccag aggcgaaggc ggctcgctgg gcttggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000585
VERSION     KHUT01000585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           96 g           40 t
ORIGIN      
        1 cacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agagcgtgta ggcggtgctg
       61 taagtcaacc gtgaaagctc ccggctaaac tgggagaggt cggatgagac tgcagcgcta
      121 gtgggtcgta gaggagtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcactctgga cgacatcaga cgctgagacg cgacagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000586
VERSION     KHUT01000586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c          100 g           36 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 cacgtcggga gtgaaagccc ggggctcaac cccggaatgt ctctcgagac tgccgagcta
      121 gagtgcggaa gaggcacctg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccaga ggcgaaggcg gggtgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000587
VERSION     KHUT01000587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaactg cattggaaac tggctgacta
      121 gagtgcggta gaggggtgtg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatctgt ggcgaaggcg acaccctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000588
VERSION     KHUT01000588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtggga
       61 taagtcggat gtgaaatctc ggagcttaac tccgaaactg cattggaaac tgtcctgcta
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000589
VERSION     KHUT01000589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattgg
       61 taagtccgtg gtgaaatccc cgagcttaac ttgggaactg ccatggatac tattaatctt
      121 gaatgtcgtg gagatgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgttacg cgaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000590
VERSION     KHUT01000590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggttttg
       61 aaagtttgct gtcaaagcgc gaggctcaac ttcgtatcgg cggtgaaaac ttcaagacta
      121 gagctaggta ggggtcacgg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagc ggcgaaagcg cgtgactggg cctgagctga cactcatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000591
VERSION     KHUT01000591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggccaac
       61 taagtcatac gtgaaatccc tcagcttaac tggggaactg cgcctgatac tggttggctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000592
VERSION     KHUT01000592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           84 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcagtg gtgaaagccc atcgctcaac gatggaactg ccattgatac tgataggctt
      121 gagtacagac gaggttggcg gaattgatgc tgtagcggtg aaatgcatag atagcatcaa
      181 gaacaccgat tgcgtaggca gctaactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000593
VERSION     KHUT01000593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggttttt
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaactg ccattgaaac tgtgaaactt
      121 gaggacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgtttctga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000594
VERSION     KHUT01000594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcacc cgtggaactg ctgggcatac tggcgatctt
      121 gggccaacta ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg gttgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000595
VERSION     KHUT01000595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           92 g           48 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttgcc
       61 caagtcgggt gtgaaagcct tgagctcaac tcaagaaatg cactcggtac tgggtgacta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000596
VERSION     KHUT01000596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggttggg
       61 tgtgtcagat gtgaaatccc gaggctcaac ctcggaactg cgtctgaaac tactcgacta
      121 gagtactgga gagggtaacg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gttacctgga cagttactga cgctcaggca cgaaagcatg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000597
VERSION     KHUT01000597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttatg
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaattg catttgagac tgtgtagcta
      121 gggtgcggaa gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcttcctggt ccagcaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000598
VERSION     KHUT01000598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggcgttg
       61 caagtcgagt gtgaaatccc cgagcttaac ttgggaattg cgctcgaaac tacgatgctc
      121 gagtatggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccttctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000599
VERSION     KHUT01000599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgtt gtaaaagtca aaggctcaac ctttgaaagc cgatggatac tgccgggctc
      121 gagtccggaa gaggcgagta gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaatacccat agcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000600
VERSION     KHUT01000600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           76 g           62 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaataa
       61 taagtcagcg gtgaaagcct gtggctcaac catagaattg ccattgatac tgttattctt
      121 gagtatagtt gaagtgggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gctcactaag ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000601
VERSION     KHUT01000601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           82 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtcagcg gtgaaagttt atggctcaac catgaaattg ccgttgatac tgtcgggctt
      121 gagtgttgat ggggtacatg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcgaaggca ttgtacttat caacaactga cgctgaggca cgaaagcgcg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000602
VERSION     KHUT01000602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagtcgaac gtgaaatccc caagctcaac ttgggaactg cgtccgatac tggcaggctt
      121 gaattcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgaaattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000603
VERSION     KHUT01000603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           81 g           63 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatact ccagcttaac tggaggggtg ccattgatac tgcagggctt
      121 gagttaagtt gaagttggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gctaactaaa cttttactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000604
VERSION     KHUT01000604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           88 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggatcga
       61 tcagtcaggg gtgaaatccc agagctcaac tctggaactg cctttgatac tgtcgatctg
      121 gagtatggaa gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000605             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000605
VERSION     KHUT01000605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           93 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcagc
       61 acaagtcaat tgtgaaatct ccgagcttaa ctcggaaagg tcaactgaaa ctgtgcgact
      121 agagtgcgga aggggcaact ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaagc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000606
VERSION     KHUT01000606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           86 g           56 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgggg
       61 taagttggat gtgaaatctt ggggcttaac ctcaaaattg cattcaatac tgctccgctc
      121 gagttctgta gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg aatctctggg cagcaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000607
VERSION     KHUT01000607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 tgcgtcaacc gtgaaaacca gcagctcaac tgttggcttg cggttgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000608
VERSION     KHUT01000608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtatgca ggcggatctt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctctgatac tggggatctt
      121 gagtccggga gaggttagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctaactggc ccggtactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000609
VERSION     KHUT01000609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           59 c           87 g           44 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtcctg
       61 caagtcttcc gttaaatcca gctgctcaac agatgggctg cggaggatac tacagggcta
      121 ggaggcggga gaggcaagcg gtactttatg ggtaggggta aaatccgttg atccatagaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000610
VERSION     KHUT01000610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggacctg
       61 taagtcggga gtgaaaactc ggggctcaac tccgagcctg cttctgaaac tgcgggtctt
      121 gaggatcgga gaggagagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggc ggcgaaggcg gctctctgga cgattcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01000611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000611
VERSION     KHUT01000611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcatgt
       61 taagtttgat gtgaaagctc agggcttaac cctgaaatgg cattgaatac tagcgtgctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01000612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000612
VERSION     KHUT01000612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggatcgt
       61 taagttgggg gtgaaatccc agggctcaac cctggaactg ccttcaatac tggcgatcta
      121 gagtccggaa gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000613
VERSION     KHUT01000613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           87 g           46 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgttgggc gtgaaagccc ggggcttaac cccggaattg cacccaaaac tacatggcta
      121 gagcattgga gagggtagca gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaataccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000614             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000614
VERSION     KHUT01000614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctttg
       61 taagtctggt gtttaaccct ggggctcaac tccaggtcgc atcggaaact gcaaggcttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01000615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000615
VERSION     KHUT01000615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttct
       61 taagtccgtt gtgaaagccc tgggctcaac ctgggaatgg cagtggatac tgggaagcta
      121 gagtgcggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000616             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000616
VERSION     KHUT01000616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           95 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggactta ctgggcgtaa agggtgcgta ggtggtctgt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaatgg catctgatac tggcggactt
      121 ggagtcttgg agagggtggt ggaattcctg gtgtagcggt gaaatgcgta gagatcagga
      181 ggaacactag tggcgaaggc ggccacctgg ccaaagactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000617             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000617
VERSION     KHUT01000617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          105 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccagg
       61 caagtcggct ttgtgaaagc ccggggctca accccggagg tgcggccggg actgcttggc
      121 tggaggccgg aagaggaggg tggaataccc agtgtagagg tgaaattcgt agatattggg
      181 tggaacaccg gtggcgaaag cggctctctg gtccggacct gacgctgagg cgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01000618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000618
VERSION     KHUT01000618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagttaggg gtgaaatccc agggctcaac cctggaactg cctttaatac tggcaatctc
      121 gagtccgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000619
VERSION     KHUT01000619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           48 t
ORIGIN      
        1 tacggagggt gctagcgtta tccggaatca ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtccgat gttaaagact ggggctcaac cccagcactg cgttggatac tggcagactg
      121 gacggttgga gaggcgagtg gaattaccag tgtagcggtg gaatgcgtag atactggtag
      181 gaacacccat tgcgaaggca gctcgctgga caacacgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000620
VERSION     KHUT01000620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtgttt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaatgg cattggaaac tggatcactg
      121 gagtgcggta gaggatggcg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000621
VERSION     KHUT01000621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           85 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtgtcc
       61 taagtcagtt gtgaaatacg gcagcttaac tgtcgaggtg caattgatac tgggacactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000622
VERSION     KHUT01000622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           98 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcggat gtgaaatccc tcggctcaac cgaggaactg catccgatac tggcgggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000623
VERSION     KHUT01000623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           94 g           45 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggcggtatgt
       61 taagtctggc gtgaaagctc tcggctaaac cgggagaggt cgttggatac tggcaaacta
      121 gaggatggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccatacctga cgctaaagcg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000624             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000624
VERSION     KHUT01000624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccacc
       61 gcaagtcagt tgtgaaatct ctgggcttaa cccagaaagg tcaactgata ctgcggggct
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgatgc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000625
VERSION     KHUT01000625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           95 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgct ctgaaagtcc agggctcaac cctggaaagc gggtggatac tgccgggcta
      121 gaatccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acggtattga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000626
VERSION     KHUT01000626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c          103 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctcgt
       61 taagtggttg gtgaaatccc ggggctcaac tccggggctg ccggtcagac tggcgggcta
      121 gagcacggta ggggcagatg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg ttctgctggg ccgttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000627
VERSION     KHUT01000627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctgtt gtgaaatctc tgggctcaac tcagagtctg caacggaaac tgccgtgctt
      121 gagtatggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000628
VERSION     KHUT01000628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c           98 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcggcc gtgaaaactt ggggcttaac tccaagcttg cggtcgatac gggcagactt
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgacactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000629
VERSION     KHUT01000629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctctc
       61 cacgtcgggt gtgaaagctc ggagctcaac tctgagacgc cattcgatac gggagagcta
      121 gaggcaggta gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000630
VERSION     KHUT01000630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctagg
       61 taagtggggt gttaaatctc ccggctcaac cgggaacctg cacctcatac tgcttggctt
      121 gagtacagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg ctgatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000631
VERSION     KHUT01000631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           89 g           45 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaattg cgccctaaac tgtcgcgctc
      121 gaggaaccta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg ggttatctga cgctgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000632
VERSION     KHUT01000632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcccga
       61 taagtagggg gtgaaatctt gcggctcaac cgcaaggctg ccttctaaac tgtcgggctg
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000633
VERSION     KHUT01000633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctaat
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg catccgatac tgattggcta
      121 gagtatgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggc ttaatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000634
VERSION     KHUT01000634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcggt
       61 taagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaaaac tagccgactg
      121 gagaatggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctgga ccatttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000635
VERSION     KHUT01000635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtccgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaacgg cattggatac tggcggactg
      121 gagtgcggta gaggatagcg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatctgt ggcgaaggcg gctatctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000636
VERSION     KHUT01000636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           47 c           98 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtcgact gtgaaaacct ggggcttaac tctgggcttg cagtcgatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000637             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000637
VERSION     KHUT01000637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttctt
       61 taagtctggt gtctaagtgc ggggctcaac cccgtgatgc actggaaact ggggaacttg
      121 agtgcagaag aggagagcgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg ctctctggac tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01000638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000638
VERSION     KHUT01000638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggtcccg
       61 caagtccgct gtgaaagccc cgggcttaac ctgggaatgg cagtggaaac tatgggactt
      121 gaatctggca gaggggggtg gaatgccacg tgtagcagtg aaatgcgtag agatgtggcg
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000639
VERSION     KHUT01000639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgagg
       61 taagtcggat gtgaaatctc ggagctcaac tccgaaactg cattggaaac tacctggcta
      121 gaggtttgga ggggggactg gaatgcttgg tgtagcagtg aaatgcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000640
VERSION     KHUT01000640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          108 g           36 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggccgag
       61 caaggtcgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtggcaac tactgggctg
      121 gagcactgta ggggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000641             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000641
VERSION     KHUT01000641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           59 c           93 g           39 t
ORIGIN      
        1 gacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcccg cgtgaaatcc cccggctcaa ccggggaacg gccgggcata ctggcggtct
      121 tgagcggtct aggggtcatc ggaacaaacg gtggagcggt gaaatgcgta gatatcgttt
      181 ggaacgccga cggtgaaaac agatgactgg ggacctgctg acgctgagac acgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000642
VERSION     KHUT01000642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgca ggtggtgcgt
       61 taagtccgat gtgaaagccc tgggctcaac ctgggaatgg cattggatac tggcgcgctc
      121 gagtacggta gagggtggcg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccacctgga ccagtactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000643
VERSION     KHUT01000643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           91 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagtctgtt gtgaaatctc ccggcttaac cgggagggtg cggcggaaac tggagtgctt
      121 gagggtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccacttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000644
VERSION     KHUT01000644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtccggt gtgaaacctt ggggctcaac cccaagcctg cactggatac tgcctcgctt
      121 gagtccggga ggggagagcg gaattcctag tgtagcggtg aaatgcgcag atattaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000645
VERSION     KHUT01000645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           99 g           41 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgc
       61 tgtgtcctgt gtgaaagccc cgggctcaac ctgggaatgg catgggaaac tggcgggctg
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000646
VERSION     KHUT01000646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           91 g           48 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggacgtg
       61 taagtctatt ctgaaagctc ctggctaaac tgggagaggc gaatggagac tgcatatcta
      121 gagtgaggaa gaggaaagtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaagcg actttctggt ccttaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000647
VERSION     KHUT01000647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           98 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcgact gtgaaaaccc ggggctcaac tccgggcctg cagtcgatac gggctggctt
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000648
VERSION     KHUT01000648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtttgg
       61 caagtctgat gtgaaatcta tgggctcaac ccatagtctg catcagaaac tgccgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000649
VERSION     KHUT01000649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taagtccgct gtgaaaactc gaagctcaac ttcgagctgt cggtggaaac tatttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggt acggaactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000650
VERSION     KHUT01000650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           92 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgat
       61 taagttagat gtgaaagtcc tgggctcaac ctggggactg catttaatac tggttggctt
      121 gaattcggga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctatctgga ccgacattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000651
VERSION     KHUT01000651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          101 g           45 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcccg
       61 tatgtccgct gtgaaatcac acggctcaac cgtgttgggt cggtggaaac tgcgggactt
      121 gaggctggga ggggctggtg gaattcccca tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000652             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000652
VERSION     KHUT01000652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           98 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaaaccc gtggctcaac catgtggtct gcagtcgata cgggcaggct
      121 agaattcggt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatattg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000653             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000653
VERSION     KHUT01000653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           48 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttc
       61 taagttgagg gtgaaatccc ggggcctaac cccggaactg ccttcaaaac tggagagctt
      121 agagtacggg agaggtgagt ggaatctcta gtgtagaggt ggaattcata gatattagag
      181 ggaacaccag tggcgaaggc ggctcactgg cccgtaactg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000654
VERSION     KHUT01000654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgtacgta ggcggcgtcg
       61 taagtgtcgg atgaaatccc acggcttacc cgtggaacgg tccggcatac tgcggtgcta
      121 gaggatggta gaggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gctctctggt ccatgtctga cgctgaggta cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000655
VERSION     KHUT01000655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           94 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgcgc
       61 taagtcgggt gtgaaatccc ggggcttaac ctcgggactg cattcgatac tggcgtgctt
      121 gaggactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag agatcatgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000656
VERSION     KHUT01000656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           45 c           82 g           48 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggattgt
       61 aaagtcctct gttaaagatc ggggcttaac ccagttcaag cagtggaaac ttataatcta
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccgac agcgaaagca ctctgctggg ccgaaactga cactaagaga cgaaagctag
      241 gggagcaaaa agg
//
LOCUS       KHUT01000657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000657
VERSION     KHUT01000657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           48 c          101 g           52 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcatcc gtgaaatcct gcggcttaac tgtggggttg cggttgatac gggcaggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000658
VERSION     KHUT01000658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           45 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggccggc
       61 caagtccgat gtgaaatctt ccggcttaac tggaaaacgg catcggatac tggtcggctg
      121 gaaggtggga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctacctggc ccactcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000659
VERSION     KHUT01000659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgcgta ggcggtccga
       61 taagtcggct gtgaaagtca ggggctcaac ccttgaaagc cggccgatac tgtcggacta
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000660
VERSION     KHUT01000660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcacgca ggcggtcggc
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cactcgatac tggtcgactt
      121 gaaggcggga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ccgttcttga cgctcatgtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000661
VERSION     KHUT01000661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccatc
       61 taagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tggacggctc
      121 gagtttggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000662
VERSION     KHUT01000662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           51 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggtttga
       61 caagtctgct gttaaagtgc agagcttaac tctgtaaagg cggtggaaac tgtcaagctt
      121 gagtatggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg ctctactggg ccataactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000663
VERSION     KHUT01000663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          102 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctttt
       61 caagcgtgcg gtgaaagctc ggggcttaac tccgagtctg ccgtgcgaac tgggaggctt
      121 gagtctggta gaggtcgatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtcgactggg ccagtactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000664
VERSION     KHUT01000664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           89 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ttgggcgtaa agcgcgtgta ggcggttcag
       61 taagtccgct ctgaaagccc agggctcaac cctgggaggc gggtggaaac tactgaacta
      121 gagttcggaa gaggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac agcgaaggca gatctctggg acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000665
VERSION     KHUT01000665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggattga ctgggcgtaa agggcgcgta ggcggcttgt
       61 acagtcagac gtgaaattcc tgggctcaac ctgggggctg cgtttgatac gtgcgggctt
      121 gagttcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000666
VERSION     KHUT01000666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           92 g           47 t
ORIGIN      
        1 tacgtaggtg acaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 caagtcgagt gtgaaatccc ccggctcaac tggggagggt cactcgatac tgatcgactc
      121 gaagacagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000667
VERSION     KHUT01000667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           87 g           47 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcttgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac taccgagctt
      121 gagtaggcta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg acctaactga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000668
VERSION     KHUT01000668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggctttc
       61 taagtcagag gtgaaatcct ggagctcaac tccagaactg cctttgatac tggaaagctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000669
VERSION     KHUT01000669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           87 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaagg
       61 aaagtcagat gtgaaatccc caggctcaac ctgggaactg catttgaaac tccctttctt
      121 gagtaccgga gaggaaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000670
VERSION     KHUT01000670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatatg
       61 taagtccgtg gtgaaatctc tgggcttaac ccggaaactg ccatggatac tatgtgtctt
      121 gaatgttgtt gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaag caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000671
VERSION     KHUT01000671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtgagg
       61 gaagtttgag gtgaaatctc caggctcaac ttggaaattg ccttgaagac tcccttgctt
      121 gagggtcgta gaggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg ggtctctgga cgacatctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000672
VERSION     KHUT01000672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           44 c           83 g           47 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttat
       61 taagtctact gttaaagatc agggcttaac cctgagcagg caatagaaac taataagcta
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01000673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000673
VERSION     KHUT01000673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           83 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtccta
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgcaggactt
      121 gagtagagtt gaggttggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gctaactaag ctctaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000674
VERSION     KHUT01000674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccat
       61 taagtcagtg gtgaaagccc ggggctcaac cccggaaccg ccattgatac tggtgggctt
      121 gagtatggac gaggttggcg gaattgaggg tgtagcggtg aaatgcttag ataccctcaa
      181 gaacaccgat tgcgtaggca gctaactagg ccataactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000675
VERSION     KHUT01000675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           96 g           44 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggccacg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgcgtggctc
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000676
VERSION     KHUT01000676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgcca
       61 caagtcactt gtgaaaactc tgggctcaac tcagagcctg caagcgaaac tgtggtgctg
      121 gagtatggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000677
VERSION     KHUT01000677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           97 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtccgct ctgaaagccc ggggctcaac cccgggaggc gggtggatac tgtcaagctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000678
VERSION     KHUT01000678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac tcggaaactg ccgttgatac tatctgtctt
      121 gaatattgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgctaca catatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000679
VERSION     KHUT01000679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctatg
       61 caagtcggat gtgaaagccc tcggctcaac cggggaagtg cacccgaaac tgcaaagcta
      121 gagtctcgga gaggatcgtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcgatctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000680
VERSION     KHUT01000680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           83 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggcaaa
       61 taagtcagtg gtgaaagccg gcagctcaac tgtcgaattg ccattgatac tgtttgtcta
      121 gagtatcgtt gaggtaggcg gaatgagtcg tgtagcggtg aaatgcatag atatgactca
      181 gaacaccgat agcgtaggca gcttactaag cgataactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000681
VERSION     KHUT01000681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           90 g           43 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgcacgta ggcggcgcag
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tgcagtgcta
      121 gaattctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga cagacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000682
VERSION     KHUT01000682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgcctggctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000683
VERSION     KHUT01000683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           91 g           51 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccga
       61 caagtcaggg gtgaaatcct gcagcttaac tgcagaagtg cctttgatac tgtcggtctt
      121 gagggagata ggggtgtgcg gaactcacag tggagcggtg aaatgcgttg atattgtgag
      181 gaacgccgga ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000684
VERSION     KHUT01000684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagtccgat gtgaaagccc tgggctcaac ctgggaatgg cattggatac tggcgagcta
      121 gagtgtgata gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga tcaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000685
VERSION     KHUT01000685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 caagtcaggt gtgaaatccc ggggctcaac cccggaactg catttgatac tgccttgctg
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000686
VERSION     KHUT01000686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtcggat gtgaaatctc cgggctcaac ccggaaatgg cattggaaac taccttgctg
      121 gaggatttga ggggggattg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg aatccctgga aaatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000687
VERSION     KHUT01000687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacca
       61 caagtaccgt atgaaatctc cttgctcaac gaggagggta taaggtagac tgtggagcta
      121 gaggagggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccctacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000688
VERSION     KHUT01000688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtgagt
       61 taagtctgat gtgaaatccc cgggctcaac ctgggaatgg cattggatac tggctcgcta
      121 gagtgcggta gagggtggtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccacctgga ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000689
VERSION     KHUT01000689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           96 g           42 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccact
       61 taagtcctgc gtgaaatcct gcggctcaac cgcaggcggt cgtgggaaac tgggtggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000690             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000690
VERSION     KHUT01000690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          103 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcctgt
       61 ggaagtctgc ggtgaaagcc cggcgctcaa cgccgggtcg gccgtggata ctctcgggct
      121 tgagctcact agaggcagat ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggtctgctgg ggtgatgctg acactgatgc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000691
VERSION     KHUT01000691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 tgagtcagac gtgaaatccc caggcttaac ttgggaactg cgtctgatac tgcaaggctc
      121 gagtgcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000692
VERSION     KHUT01000692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           46 c           99 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaatcca ctggcttaac tggtggcttg ctttcgatac gggcagactg
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg catgtcctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000693
VERSION     KHUT01000693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcaacg
       61 taagtcagaa gtgaaagctc tcggctcaac tgaggaattg cttttgatac tgcgtagctt
      121 gaataaggtt gaggtcagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccaat tgcgaaggca gctggctaga cctttattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000694
VERSION     KHUT01000694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccaga
       61 taggtctgct gtgaaaactc gaggctcaac ctcgagctgt cggcggaaac catccggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacctgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000695
VERSION     KHUT01000695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           90 g           57 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggagtta ctgggcgtaa agagtacgta ggcggtttgc
       61 caagtttggt gtgaaatctc ctggcttaac tgggagggtg cgccgaatac tggcgggctt
      121 gagtattgga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga caatcactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000696
VERSION     KHUT01000696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtctgc
       61 tgcgttcgga gtgacagtcc tcagcttaac tgaggaagcg cttggaagac gggcagactt
      121 gagggctcac gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgtaggca gtgtcctggg gagtcgctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000697
VERSION     KHUT01000697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtctgcc gtgaaagccc cgggctcaac ctgggaatgg cggtggatac tggcggacta
      121 gagtgcggta gagggtggtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccacctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000698
VERSION     KHUT01000698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           81 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctat
       61 taagtcagtg gtgaaatact ccagcttaac tggaggggtg ccattgatac tgatggactt
      121 gagtcaagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccaat agtgaagaca gcttactagg cttaaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000699
VERSION     KHUT01000699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcttt
       61 taagtctgat gtgaaagccc acggctcaac cgtggaaggt cattggaaac tggaagactt
      121 gagtgcagaa gagaagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctttggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000700
VERSION     KHUT01000700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           86 g           50 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg cttggtatac tgggtgtctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000701
VERSION     KHUT01000701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctatg
       61 caagtcgggc gtgaaagccc tcggctcaac cgaggaagtg cactcgaaac tgcgtagcta
      121 gagtctcgga gaggaaggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000702
VERSION     KHUT01000702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcgggctgg
       61 aaagttgggg gtgaaatccc ggggctcaac cccggaatgg ccttcaaaac tcccagccta
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000703
VERSION     KHUT01000703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggttacg
       61 caagtcggtt gtgaaaatcc ggggctcaac cccggacctg catccgatac tgcgtgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000704
VERSION     KHUT01000704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta gtcggctttg
       61 caagtcgggt gtgaaatccc cgggctcaac ctgggaactg catccgagac tgcattgcta
      121 gagtatggga gagggaagcg gaatttctgg tgtagcagtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaggcg gcttcctgga ccaatactga cgatcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000705
VERSION     KHUT01000705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattat
       61 taagtccgtg gtgaaatctc caagcttaac ttggaaactg ccatggatac tattagtctt
      121 gaatgttgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000706
VERSION     KHUT01000706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           91 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtcctg
       61 caagttgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccaaaac tacagaactt
      121 gagtaccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000707
VERSION     KHUT01000707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cacgtctgcc gtgaaagccc ggagcttaac tccgggtctg cggtggatac gggcaaacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000708
VERSION     KHUT01000708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           49 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtcttc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tgggaggctg
      121 gagtatggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtagacg gcttactgga ccataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000709
VERSION     KHUT01000709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatatt
       61 taagtcaggg gtgaaatccc agggctcaac cctggaactg cctttgatac tggatgtctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000710
VERSION     KHUT01000710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggaccgg
       61 aaagtcagag gtgaaatccc agggctcaac cttggaactg cctttgaaac tcctggtctt
      121 gaggtcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000711
VERSION     KHUT01000711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           58 c          107 g           39 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgcgtgca ggtggctcat
       61 tacgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac ggatgagctt
      121 gagggtcgca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg cgacacctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000712
VERSION     KHUT01000712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggccatt
       61 taagctagat gtgaaagccc cgggcttaac ctgggaattg catttagaac tggatggcta
      121 gagtatggaa gaggagtgtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg acactctggt ccaatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000713
VERSION     KHUT01000713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           96 g           45 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtgcgta ggcggctgcg
       61 caagtcggtt gtgaaacccc ggggctcaac cccggaactg catccgatac tgcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggca cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000714
VERSION     KHUT01000714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           56 c           91 g           39 t
ORIGIN      
        1 gacaggggat gcaagcgtta tccggaatga tcgggcgtaa agcgtctgta ggtagctcct
       61 caagtccgcc gtcaaatccc agggctcaac cctggaaggg cggtggaaac taccgagccg
      121 gagtacggta ggggcagggg gaatttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacaccaat ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01000715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000715
VERSION     KHUT01000715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtgggtt gtgaaaactg gaggctcaac cttcagacgt cgacccaaac cccctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000716
VERSION     KHUT01000716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          101 g           47 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcgacc gtgaaaaccc gcagcttaac tgcgggcttg cggttgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000717
VERSION     KHUT01000717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 taagtggaat gtgaaatccc ccggcttacc cggggaactg catcccatac tgctggactt
      121 gagtgcggga gaggatggag gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg tccatctggc ccgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000718
VERSION     KHUT01000718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggctct
       61 taagtcgagg gtgaaatcct gtggctcaac cacagaacgt ccttcgatac tgggagcctg
      121 gagttcgaga gaggttggtg gaattgcgag tgtagaggtg aaattcgcag atattcgcag
      181 gaacaccagt ggcgaaggcg accaactggc tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000719
VERSION     KHUT01000719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c          102 g           45 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtaa agggagcgta ggtggttagg
       61 taagccctcc gggaaatctt caggcttaac ctgaaaaggt cgggggggac tgcctggcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtttctga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000720
VERSION     KHUT01000720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgaag
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaactg catccgatac tgcttcgcta
      121 gagtatggta gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000721
VERSION     KHUT01000721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           93 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcggcc
       61 caagtcaggt gtgaaatccc cgggcttaac ctgggaattg cgcttgaaac taggtcgcta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000722             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000722
VERSION     KHUT01000722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaatttc gaggctcaac ctcgaaacgc cactcgatac tgccatgact
      121 ggagtccggt agaggagtgc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000723
VERSION     KHUT01000723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           76 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcact
       61 taagtctggg gtgaaatctc cgagctcaac tcggaaactg ccttggatac tatttgcctt
      121 gaattatgtt gaggttggcg gaatataaca tgtagcggtg aaatgcatag atatgttata
      181 gaacaccgat tgcgaaggca gctaactaag catatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000724
VERSION     KHUT01000724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggtccac
       61 taagtcgagt gtgaaatccc ccggctcaac cggggaatgg cgctcgatac tggtgggcta
      121 gaggtcggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga tcgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000725
VERSION     KHUT01000725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           90 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaattg cattcgatac tgcttagcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000726
VERSION     KHUT01000726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttag
       61 taagttgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccaaaac tgcttggctt
      121 gagtaccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccacctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000727
VERSION     KHUT01000727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agagctcgta ggtggctctg
       61 taagtcggag gtgaaagacc acggctcaac cgtggaaacg ccttcgaaac tgtggagctt
      121 gaggctggga gagggaatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gattcctgga ccagttctga cactgaggag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000728
VERSION     KHUT01000728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgagta ggtggccatc
       61 taagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tggacggcta
      121 gagtatggga gagggatgta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccattactga cactgaaccg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000729
VERSION     KHUT01000729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           88 g           59 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 taagtctggt gtgaaatctc tgagctcaac tcggaaattg cattggatac tgcgttgctt
      121 gaggattgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000730
VERSION     KHUT01000730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           94 g           41 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggccctg
       61 taagtccgct ctgaaagtca aaggctcaac ctttgaaagc gggtggatac tgcagggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000731
VERSION     KHUT01000731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           92 g           55 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttgg
       61 taagtctgat gtgaaatcta tgggctcaac tcatagtctg catcggaaac tgccgggctt
      121 gagtgtggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000732
VERSION     KHUT01000732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggttctt
       61 gaagtccgct gtgaaagccc cgggcttaac ctgggaatgg cagtggaaac tggaggactt
      121 gaattgagca gaggggggtg gaatgccgcg tgtagcagtg aaatgcgtag agatgcggcg
      181 gaacaccgat ggcgaaggca gccccctggg cttaaattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000733
VERSION     KHUT01000733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           96 g           38 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgt
       61 caagtcccgt gtgaaagccc ccggctcaac cggggaactg cacgggaaac tagcaggctt
      121 gagttcggga gagggaagcg gaatttcggg tgtagcggtg aaatgcgcag atatccgaag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000734             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000734
VERSION     KHUT01000734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c          101 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatta ttgggcgtaa agcgcacgta ggcggttctt
       61 taagtctcat ggtgaaaggc tggggctcaa ccccaagcaa gccggggata ctggaggact
      121 ggagacaggc agaggccagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggctggctgg gcctgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000735
VERSION     KHUT01000735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctgtt
       61 caagtcgatt gtgaaaactc ggagctcaac tccgagcctg cgttcgatac tggacggcta
      121 gagtctggta gggggacctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gggtcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000736
VERSION     KHUT01000736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggccact
       61 caagtcgatt gtgaaaactc ggagctcaac tccgagcccg cgatcgatac tgggcggcta
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000737
VERSION     KHUT01000737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctatt
       61 caggtcaggt gtgaaagccc tcggctcaac cgaggaactg cgcttgaaac cggatggctc
      121 gagtcaacta gaggtgcacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgcactggg gttggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000738
VERSION     KHUT01000738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggtacta
       61 tgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tgtagaacta
      121 gagtagatga gagggtgatg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gtcacctggc atcatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000739
VERSION     KHUT01000739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggcctga
       61 taagtcggat gtgaaacccc tcggcttaac cgaggatccg cagccgaaac tgccaggctt
      121 gagtctagga gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ctagtactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000740
VERSION     KHUT01000740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggatctt
       61 taagtcagac gtgaaatccc cgagcttaac ttgggaatgg cgtttgaaac tggatgtcta
      121 gagtgcgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg atgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000741
VERSION     KHUT01000741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           95 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 ttagtcgagt gtgaaagccc tgggcttaac ccgggaagcg cgcttgatac tgcagggctt
      121 gagtgtggga gaggatgatg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg atcatctgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000742
VERSION     KHUT01000742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgccg
       61 caagtcactt gtgaaatctc cgggctcaac tcggaacggc caagtgatac tgcggtgcta
      121 gagtgcggaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000743
VERSION     KHUT01000743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           89 g           51 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttgg
       61 caagtcttgt gtgaaagccc acagcttaac tgtggaagtg cataagatac tgctgaattt
      121 gagttcagga gaggaaggca gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000744
VERSION     KHUT01000744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgtgta ggcggtcttt
       61 taagtctgat gtgaaagccc tgggctcaac ccaggaagtg cattggatac tgggagactt
      121 gaatacggga gagggtagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000745
VERSION     KHUT01000745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacggagggg actagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatttg
       61 caagtcgggg gtgaaagccc gaggctcaac ctcggaactg ccttcgatac tgcaaatctc
      121 gagtccgaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000746
VERSION     KHUT01000746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           85 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatatc tcagcttaac tgagagggtg ccattgatac tgcggggctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000747
VERSION     KHUT01000747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          101 g           42 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggggagg
       61 taagtcgggt gtgaaatccc tcggcccaac cgaggaactg cgcccgagac tgcttctctt
      121 gagtcccgga gaggaagatg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gtcttctgga cggtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000748
VERSION     KHUT01000748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatctgtctt
      121 gaatatcctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg ggattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000749
VERSION     KHUT01000749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c          101 g           43 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agagtgcgta ggtggtgagg
       61 taagacctgt gggaaagacc tgggcttaac ccaggaaggt cgcaggtgac tgcctcgctt
      121 gaggacggga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaaggccggt ggcgaaggcg gctctctgga acgttcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000750
VERSION     KHUT01000750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaagtcc gaggctcaac ctcggatctg cagtgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca ggtctctggg ccacaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000751
VERSION     KHUT01000751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggtggctatg
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaactg cattcgatac tgcacagcta
      121 gagtatggga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggc ccaatactga cactcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000752
VERSION     KHUT01000752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           89 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctatg
       61 taagtctgct gttaaagagt gaggctcaac ctcataagag cagtggaaac tacatagcta
      121 gagtacgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaggcg ctctgctagg ccgtaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000753
VERSION     KHUT01000753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggttaca
       61 taagtctgtt gtgaaagtcg gaggctcaac cttcgaaagc cgatggatac tgtgtgactt
      121 gagtacggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctctctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000754
VERSION     KHUT01000754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctt
       61 caagtttggg gtgaaatcct ccggctcaac cggagaactg cctcgaatac tgaaggtctc
      121 gagggggata ggggcatgcg gaactacggg tggagcggtg aaatgcgttg atatccgtag
      181 gaactccggt ggcgaaggcg gcgtgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000755
VERSION     KHUT01000755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           61 c           86 g           42 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtccca
       61 caagtcctcc gttaaatcca actgcttaac agatgggccg cggaggatac tatggggcta
      121 ggaggcagga gaggcaagcg gtactcagtg ggtaggggta aaatccgttg atccactgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga atgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000756
VERSION     KHUT01000756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           91 g           45 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggcgtaa agcgcacgca ggcggttccg
       61 aaagtcgaat gtgaaagctc ctggcccaac tgggagaggc cgttcgaaac ttcgggactt
      121 gaaggcggga gaggcagatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaagcg atctgctggc ccgcccttga cgctcaggtg cgaaagctag
      241 gggagcgacc ggg
//
LOCUS       KHUT01000757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000757
VERSION     KHUT01000757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           94 g           43 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggcggtcgtg
       61 caagtcggat gtgaaaatcc cgggctcaac ccgggacctg cacccgatac tgctcgactc
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000758
VERSION     KHUT01000758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcag
       61 tatgtcgggt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgctggactt
      121 gaatactgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000759
VERSION     KHUT01000759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcttga
       61 aaagtttgat gtgaaagctc agggcttaac cctgaaatgg cattgaatac tgtcgggctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000760
VERSION     KHUT01000760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          105 g           39 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggaggt
       61 caagtcggtc gtgaaatctc cgggctcaac ccggaggagt cggccgaaac tggtcttctt
      121 gagggaggca gaggagtctg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggactctggg cctaccctga cgctgaggcg cgaaggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000761
VERSION     KHUT01000761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           85 g           53 t
ORIGIN      
        1 gacgagccac ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgtag
       61 caagtcggga gtgaaatacc ccagcttaac tgaggaattg ctttcgatac tgctatgctt
      121 gagtgagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gcttactggg tctctactga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000762
VERSION     KHUT01000762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaatgg cattggatac tggcaaccta
      121 gaatgtggta gagggtggcg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatccgt ggcgaaggcg gccacctgga ccaacattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000763             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000763
VERSION     KHUT01000763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          103 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggtccgg
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggagg ccaggggata ctgtcggact
      121 ggagacaggc agaggctggt agaatttcgg gtgtagcggt ggaatgcgta gagatccgaa
      181 ggaataccgg aggcgaaggc ggccagctgg gcctgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000764
VERSION     KHUT01000764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           94 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacatt
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tgggtgtctg
      121 gaggtcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000765
VERSION     KHUT01000765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           85 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggt
       61 taagtctgac gtgaaatctc cgagcctaac tcggaaactg catcggaaac tatccggctc
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000766
VERSION     KHUT01000766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           95 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta gtcggaatta ctgggcgtaa agcgtgcgta ggcggttggt
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaatgg cactcgatac tggccagctg
      121 gagtaaggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccatctgga ccattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000767
VERSION     KHUT01000767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          101 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttgg
       61 taagtagggg gtgaaatctt gcggctcaac tgcaaggctg cctcctaaac tgcctggctg
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000768
VERSION     KHUT01000768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatg
       61 caagccagac gtgaaagccc cgggcttaac ctgggaatgg cgtttgggac tgcatggcta
      121 gagtacgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg atgatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000769
VERSION     KHUT01000769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcttg
       61 taagtccgtg gtgaaatctc caagcttaac ttggaaactg ccgtggatac tataagtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttgctaca caattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000770
VERSION     KHUT01000770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta gacggttctg
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaactg cattcgagac tgcagtactg
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg actgcctggc ccaatactga cgttcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000771
VERSION     KHUT01000771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 cgtgtctgat gtgaaagccc cgggctcaac ctgggaatgg cattggaaac tggcggacta
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000772
VERSION     KHUT01000772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           98 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggctctg
       61 ttagtcaggc gtgaaattcc tgggctcaac ctggggactg cgcttgatac ggcggggctt
      121 gaggatggaa gagggtcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000773
VERSION     KHUT01000773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctcg
       61 tgtgtcgggc gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tgcaaggctg
      121 gagtactgga gaggatggtg gaatgctcgg tgtagaggtg aaattcgtag atatcgagcg
      181 gaacaccggt ggcgaaggcg gccatctgga cagtcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000774
VERSION     KHUT01000774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           92 g           44 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtttcg
       61 aaagtccacg gtgaaagctc ccggcccaac tgggagaggc ccgtggaaac tccggaactt
      121 gaaggcagga gaggcagatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaggcg atctgctggc ctgttcttga cgctcaggcg cgaaagctag
      241 gggagcgacc ggg
//
LOCUS       KHUT01000775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000775
VERSION     KHUT01000775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           84 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtaaggt gtgaaatccc ggagctcaac tccggaactg cgccttaaac tacatggcta
      121 gagtattgga gagggtaacg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gttacctgga caattactga cgctcaggca cgaaagcatg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000776
VERSION     KHUT01000776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 taagtctcgt gtgaaagccc tcggcttaac tgaggatctg cacgggatac tgcgaggctt
      121 gagttcggga gaggaaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccgacactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000777
VERSION     KHUT01000777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           92 g           44 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattca ttgggcgtaa agagcacgca ggcggtttgg
       61 caagtcggat gtgaaaaccc gaagctcaac ttcgggcctg catccgaaac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000778
VERSION     KHUT01000778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggttga
       61 caagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgtcagtctt
      121 gaggcatcta gaggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgcccat ggtgaaaaca ggttgctggg gatgtactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000779
VERSION     KHUT01000779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           98 g           41 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggttgcg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcgcgactg
      121 gagtgatgca gaggtcgatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtcgactggg catctactga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01000780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000780
VERSION     KHUT01000780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacggaggga gctagcgtta ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtaagag gtgaaagcct ggtgctcaac accagaactg ccttttagac tgcatcgctt
      121 gaatcatgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000781             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000781
VERSION     KHUT01000781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           88 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgggtcgcgg gtgaaatcct ccagcttaac tggagaactg ccctcgatac cactgcggct
      121 cgaggaagga aggggcaatt ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgcctg tggcgaaagc gaattgctgg tccttttctg acgctgatgc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000782
VERSION     KHUT01000782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcggac gtgaaatccc caggcttaac ttgggaactg cgtccgatac tggagggctt
      121 gagtgcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000783
VERSION     KHUT01000783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           99 g           41 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggtcttg
       61 tgcgtcggcg ctgaaatcac acggctcaac cgtgtggcgc gtgccgatac gacgagactt
      121 gaggctctga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc agagttctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01000784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000784
VERSION     KHUT01000784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           93 g           49 t
ORIGIN      
        1 tacgtaggtg tcaagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggcttgc
       61 caagttcgat gtgaaatctc ccggctcaac tgggagggtg catcgaaaac tggcaggctt
      121 gaggcaggga gagggatgtg gaattccagg tgtagtggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01000785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000785
VERSION     KHUT01000785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c          101 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cgcgttgttc gtgaaaaccg ggggcttaac cctcggcgtg cgggcgatac gggcagactt
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000786             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000786
VERSION     KHUT01000786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttggg
       61 caagtcacgt gtgaaatctc cacgctcaac gtggaggcgc cacgtggtac tgccctgact
      121 agagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000787
VERSION     KHUT01000787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           90 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggtggtgtcg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tgcgatgctg
      121 gagtatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccattactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000788
VERSION     KHUT01000788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           98 g           40 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggaagtg
       61 taagtcggaa gtgaaatccc gccgctcaac ggcggggctg cttcggatac tgcacctctg
      121 gggaccggta gaggctggta gaattgctgg tgtagcggtg gaatgcgtag agatcagcaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000789
VERSION     KHUT01000789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           81 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaatat
       61 taagtcagtg gtgaaatcct gcagctcaac tgtagacttg ccattgatac tggtattctt
      121 gagtgcgctt gaagtaggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccaat tgcgaaggca gcttactaag gcgatactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01000790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000790
VERSION     KHUT01000790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          102 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcga
       61 gtagtcaacg gtgaaagccc ggggctcaac cccgggtcgg ccgtcgatac tctcgtgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000791
VERSION     KHUT01000791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtcggtt gtgaaaactg gaggctcaac cttcagaggt cgaccgaaac catctggcta
      121 gagtccggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaagcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000792
VERSION     KHUT01000792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           93 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagtcagat gtgaaagtcc acggctcaac cgtgggagtg catttgaaac tggcagactt
      121 gagtattgga gggggtagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg actacctggc caaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000793
VERSION     KHUT01000793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           82 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtgggagtt
       61 taagtgagat gtgaaaccgt gtggctcaac catatcgttg catctcatac tggacaccta
      121 gaggacggca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000794
VERSION     KHUT01000794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           98 g           39 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcga
       61 caagtcccgc gtgaaagccc ccggctcaac tggggaactg cgcgggaaac tgtcggactt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000795
VERSION     KHUT01000795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggcgtaa agagcgcgta ggcggttccg
       61 taagttggtt gtgaaagttc gaggctcaac ctcgaaactg cgtccaatac tgcggggctg
      121 gagtgcaaga gaggcaagtg gaatttgcgg tgtagtagtg aaatgcgtag atatcgcaag
      181 gaacaccagt ggcgaaggcg gcttgctagc ttgtaactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000796
VERSION     KHUT01000796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgag gtgaaatctc cgagctcaac tcggaaactg ccttggatac tatccggctt
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000797
VERSION     KHUT01000797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           88 g           44 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatca ttgggcgtaa agcgtccgca ggtggcactt
       61 caagtctgcc gtcaaagacc gtagctcaac tacggaaagg cggtggaaac tgaagcgcta
      121 gagtgcagta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctgctgga ctgcaactga cactcatgga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000798
VERSION     KHUT01000798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcataa agggcgcgta ggcggctcca
       61 aaagtcaggg gtgaaatccg gcagcttaac tgtcgcagtg cctttgatac tgtggagcta
      121 gagggaggta ggggtctgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000799
VERSION     KHUT01000799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          105 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctact
       61 taagcgtgtg gtgaaagtcc ggggctcaac cccggatcgg ccgtgcgaac tgagtggctc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000800
VERSION     KHUT01000800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcagcagcg
       61 tgtgtcgggc gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tgcgcagcta
      121 gagtctcgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000801
VERSION     KHUT01000801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           96 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggtggtttgc
       61 taagttcgac gtgaaagcgc ctggcttaac tgggcgaggt cgttggagac tggcagacta
      121 gaggtgagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc cttggcctga cgctcatgtg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000802
VERSION     KHUT01000802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           99 g           43 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcacga
       61 caagtcgctt gtgaaatctc cgggcttaac tcggagcggc caagcgatac tgtcgcgcta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000803
VERSION     KHUT01000803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatttg
       61 ccagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac agcaagtctt
      121 gagttcgaga gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000804
VERSION     KHUT01000804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gccagcgttg ttcggaatta ctgggtgtaa agggttcgta ggcggtgcgg
       61 caagttggga gtgaaatctc tgggctcaac ccagagacgg cttccaaaac tgccgtgctt
      121 gagtgtgaga gaggctcgtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaagcg gcgagctgga tcactactga cgctgaagaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000805
VERSION     KHUT01000805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtgagt
       61 ttagccagct gtgaaatccc cgggctcaac ctgggaattg cagttggaac tggctcgcta
      121 gagtatggta gaggggtgtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaggcg acaccctgga tcaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000806
VERSION     KHUT01000806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          103 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggtcgta
       61 cgcgtttcgg gtgacagcgg gtggcttaac tacccgagtg ttcgaaagac ggtacgactt
      121 gaggactcga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc gagtgactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000807
VERSION     KHUT01000807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc cgagcttaac ttggaaactg ccattgatac tattaatctt
      121 gaatattgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccaat tgcgaaggca gctggctaca catatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000808
VERSION     KHUT01000808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           88 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgttctat
       61 caagtcttgt gtgaaagccc caagcctaac ttgggaagtg cacaagatac tggaagaata
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000809
VERSION     KHUT01000809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgat
       61 caagtcggat gtgaaagccc ccggctcaac tggggagggt cattcgatac tggtcgactc
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000810
VERSION     KHUT01000810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtctta
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgcagtg cctttgatac tgtaggactt
      121 gaatacactt gaagtgggcg gaataagaca agtagcggtg aaatgcatag atatgtctta
      181 gaactccgat tgcgaaggca gctcactaag gtgtgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000811
VERSION     KHUT01000811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          103 g           41 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggggagg
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaacgg cgcccgaaac tgcttctctt
      121 gagtcccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000812
VERSION     KHUT01000812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcgg
       61 aaagttgggg gtgaaatccc ggggctcaac cccggaactg ccttcaaaac tatcggtctg
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000813
VERSION     KHUT01000813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           83 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttgat
       61 taagtcagcg gtgaaagttt tgggctcaac ccagaaattg ccattgatac tggtcgactt
      121 gagtattgca ggggtacatg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgaaggca ttgtactggg caaccactga cgctgatgca cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000814
VERSION     KHUT01000814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcggat gtgaaagccc ggggcttaac cccgggtctg cattcgatac gggctagcta
      121 gagtgtggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gatctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000815
VERSION     KHUT01000815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           95 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggtggtcctc
       61 aaagtcggct gtgaaatgcc acggcttaac cgtggaggtg cagttgatac tggaggactt
      121 gagttccgga gaggaagacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gtcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000816
VERSION     KHUT01000816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagtcggac gtgaaatccc tcggctcaac cggggaactg cgtccgatac tggcgggctt
      121 gaattcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000817
VERSION     KHUT01000817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccagg
       61 taagttcggt gtgaaagctc ggagcttaac tccgaaatgg cattgaatac tatctggcta
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01000818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000818
VERSION     KHUT01000818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tgacgatctc
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000819
VERSION     KHUT01000819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           93 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 caagtcggtt gtgaaagccc agggcctaac cctggaagtg caaccgagac tgttgaattt
      121 gagtacagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgtgactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000820
VERSION     KHUT01000820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggcttg
       61 taagtcaggg gtgaaatcct acagcttaac tgtagaactg cctttgatac tatgagtctt
      121 gaatattgtt gaggtaggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gcttactaag caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000821
VERSION     KHUT01000821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcggat gtgaaagccc tcggctcaac cgaggaagtg cgttcgaaac tgcagagctt
      121 gagtaccgga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggtcactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000822
VERSION     KHUT01000822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           85 g           48 t
ORIGIN      
        1 tacgggggat gcaagcgtta tccggaatta ttgggcgtaa agggtcctga ggtggctagc
       61 caagtctatt gtgaaatatc agggcttaac cctgaacatg cggtggaaac tagaaagctt
      121 gagtacagta ggggcggagg gaatttccgg tggagcggtg aaatgcatag atatcgggaa
      181 gaacaccaac ggcgaaagca ctccgctggg ctgatactga cactgacaga cgaaagctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01000823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000823
VERSION     KHUT01000823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           61 c          108 g           38 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgtacgca ggcggcggca
       61 ttggcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac cgtgccgctt
      121 gagggcagca ggggttggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccaactggg ctgaccctga cgctgaggta cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000824
VERSION     KHUT01000824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           89 g           45 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggccgcacgg
       61 taagtcagag gtgaaatccc ggagctcaac tccggaatgg cctccgatac tgccgtgctc
      121 gaggactgga gaggagtctg gaattcacag tggagcagtg aaatgcgtag atattgtgag
      181 gaacactagt ggcgaaggcg agactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000825
VERSION     KHUT01000825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcggct gtgaaagcct ggggctcaac cccagagggt cggccgatac tggcaaacta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000826
VERSION     KHUT01000826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctttt
       61 caagtcgggc gtgaaatctc cgggcttaac ccggaagggt cgttcgatac tgttgagcta
      121 gagtgtggaa gaggcaacta gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaatacctga ggcgaaggcg ggttgctggg ccaacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000827
VERSION     KHUT01000827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 tgtgtctgtg gtgaaagccc cgggcttaac ctgggaatgg ccatggaaac tggcgagctg
      121 gagtctggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaagactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000828
VERSION     KHUT01000828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           85 g           52 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agcgtatgta ggcggttagg
       61 taagttactt gtgaaatctc cgagcttaac tcggaacggc caagtaatac tgccaaacta
      121 gagtgcagaa ggggcaattg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaggcg aattgctggg ctgacactga cgctgagata cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000829
VERSION     KHUT01000829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattca ttgggcgtaa agagcgcgca ggcggcttgg
       61 taagtcggat gtgaaaaccc agggctcaac cctggacgtg catccgatac tgcccggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01000830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000830
VERSION     KHUT01000830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c          100 g           47 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 cgagtgaggc gtgaaagtcc tgggctcaac ctggggacgg cgcttcagac tgtctggctt
      121 gagtccggaa gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggt ccggaactga cgctgaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000831
VERSION     KHUT01000831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggctctg
       61 caagtcggtt gtgaaatccc cgggctcaac ctgggaactg cgaccgagac tgcattgcta
      121 gagtatggga gaggaaagcg gaatttctgg tgtagcagtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaggcg gctttctgga ccaatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000832
VERSION     KHUT01000832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           77 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcctta
       61 taagtcagtg gtgaaatctc acggctcaac cgtgaaatgg ccattgatac tgtagggctt
      121 gaattatcag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca ggttactact ggtggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000833             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000833
VERSION     KHUT01000833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           97 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccact
       61 gcaagtcggt tgtgaaatct ccgggcttaa cccggaaagg tcaatcgata ctgcagggct
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgaagt gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000834             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000834
VERSION     KHUT01000834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           99 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtgtgt
       61 cgcgtcggcc gtgaaaacct ggggctcaac tctgggcgtg cggtcgatac gggcatcact
      121 tgagttcggc aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatactg acgctgagga gcgaaagcat
      241 gggtagcgaa cagg
//
LOCUS       KHUT01000835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000835
VERSION     KHUT01000835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtccgct gtgaaaactc gaggctcaac ctcgagaggt cggtggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaatatccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000836             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000836
VERSION     KHUT01000836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgca ggcggtctca
       61 tatgtctgct gtgaaatcac acggctcaac cgtgttcagc cgcagaaact gtgggacttg
      121 aggctgggag gggctggtgg aattccccgt gtagcggtga aatgcgtaga gatgggggag
      181 aacactcgtg gcgaaggcgg ccagctggac cagttctgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01000837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000837
VERSION     KHUT01000837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccga
       61 taagtcgggg gtgaaagtca gcggctcaac cgctggactg ccttcgagac tgttaggctt
      121 gagcatggta gaggctggcg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccattgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000838
VERSION     KHUT01000838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggtccgt
       61 taagtcatgt gtgaaatccc ccggctcaac cggggaattg cgcctgatac tggcgggcta
      121 gaggtcgata ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga tcgaaactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01000839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000839
VERSION     KHUT01000839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggctttg
       61 taagtcgaat gtgaaagccc tgggcttaac ctgggaactg catccgaaac tgcatggcta
      121 gagtaaggga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctggc ccattactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000840
VERSION     KHUT01000840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          105 g           42 t
ORIGIN      
        1 tacggaggtg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcgggggga
       61 gaagtcgggt gtgaaagccc tcggctcaac cgaggaacgg cgctcgagac tctctctctt
      121 gagtcccgga gaggacgatg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gtcgtctgga cggtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000841
VERSION     KHUT01000841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           94 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcggtt gtgaaagccc ggggcccaac cccggaattg catctgaaac tgcctcgcta
      121 gagtcccgga gaggaaggca gaattcccgg tgtaggggtg aaatccgtag atatcgggag
      181 gaataccagc ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000842
VERSION     KHUT01000842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agagcgcgca ggtggttgat
       61 taagtctgat gtgaaagccc acggcttaac cgtggagggt cattggaaac tggtcgactt
      121 gagtgcagaa gagggaagtg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gcttcctggt ctgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000843             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000843
VERSION     KHUT01000843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          104 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggctcgg
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggagg ccaggggata ctgctgggct
      121 ggagacaggc agaggctggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaatacctg aggcgaaggc ggccagctgg gcctgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000844
VERSION     KHUT01000844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgt
       61 taagtcttgc gtgaaatctc ggggctcaac cccgagcggt cgtgagaaac tggcaggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000845
VERSION     KHUT01000845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtttgt
       61 taagtcggat gtgaaatccc tcggctcaac tgaggaagtg catccgaaac tggcaagctt
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000846
VERSION     KHUT01000846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           75 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctgat
       61 taagtcagtg gtgaaatccg gcagctcaac tgtcgaaccg ccaatgatac tgatcagctt
      121 gagtacaaac gaggtatatg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaactccgat agcgaaggca ttatactaga ttgtaactga cgctgaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01000847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000847
VERSION     KHUT01000847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           86 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcagtt ctgaaatatc ccggcttaac cgggagggtg ggattgatac tgcagggctt
      121 gagtggagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gcttactaga cttcaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000848
VERSION     KHUT01000848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggacagt
       61 ttagtcagag gtgaaagccc agggctcaac cttggaattg cctttgatac tggctgtctt
      121 gagttcggga gaggtgagtg gaatgccgag tgtagaggtg aaattcgtag atattcggcg
      181 gaacaccagt ggcgaaggcg actcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000849
VERSION     KHUT01000849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           85 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gtcggttatt
       61 caagtcgggc gtgaaagccc cgggctcaac ctgggaattg cgtccgatac tgtctagcta
      121 gagttcggca gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000850
VERSION     KHUT01000850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggttaac
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggctagcta
      121 gagtgtggga gaggatagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctatctgga ccatgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000851
VERSION     KHUT01000851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           84 g           60 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgcgta gacggcattg
       61 taagtctgtt gttaaaattc ttggcctaac tgagagtctg caatggatac tgctttgcta
      121 gagggtgaga gagagaagtg gaattctcgg agtagcggtc aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggc tcatttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000852
VERSION     KHUT01000852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtttct
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggaaactt
      121 gagtgcagga gagaagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctttggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000853
VERSION     KHUT01000853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 aaagacaggc gtgaaatccc cgggctcaac ctgggaactg cgcttgtgac ttcaagacta
      121 gagtacggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ccagtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000854
VERSION     KHUT01000854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 tacgtggggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgcgta ggcggcccag
       61 caagttggat gtgaaagccc tcggcttaac cgaggaagtg cattcaaaac tactgggctt
      121 gagtacaaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gcgatctggg ttgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000855
VERSION     KHUT01000855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtctgct gtgaaatccc ggggctcaac cccgggcctg cagtgggtac gggcaagcta
      121 gagtgcggta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gatctctggg ccgctactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000856
VERSION     KHUT01000856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           85 g           57 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtagag
       61 taagttggat gtgaaatctt ggggcttaac ctcaaaattg cattcaatac tgctttgctt
      121 gagcgctgta gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg aatctctggg cagcagctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000857
VERSION     KHUT01000857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           98 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtatgta ggcggttggg
       61 caagtggcgt gtgaaagctc ccggctaaac tgggagacga caggtcagac tgccgaactc
      121 gagtccgata gaggagcgta gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaataccagt ggcgcaagcg gcgctctggg tcgggactga cgctgagata cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000858
VERSION     KHUT01000858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggagcgta ggctgtgagg
       61 taagtcgggt gtgaaatccc ggggctcaac cccggaactg cactcgatac tgctttacta
      121 gaggactgga gaggagattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacctgt ggcgaaagcg aatctctgga cagttcctga cgctgaggct cgaaggctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01000859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000859
VERSION     KHUT01000859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcatct gtgaaatctc cgggctcaac tcggagtctg caggcgaaac tgccgtgctg
      121 gagtatggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000860
VERSION     KHUT01000860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggttcgt
       61 tgcgtcagat gtgaaagccc cgggctcaac ctgggaactg catttgatac gggcgagctt
      121 gaatccaaga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccacctggc ttggtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000861
VERSION     KHUT01000861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 gacggaaggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggcttct
       61 taagtctggt gtgaaagccc agggctcagc tctggaagtg cactagaaac tgggaagctt
      121 gagtaccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggttactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000862
VERSION     KHUT01000862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttca
       61 caagtccgct gtgaaaactc gaggctcaac ctcgagctgt cggtggaaac tgtgtggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000863
VERSION     KHUT01000863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggagtta ttgggcgtaa agcgcacgca ggcggtcctg
       61 tcagtctggg gtgacagttc ccggcttcac cgggaaagtg ctctggaaac tgcaggactt
      121 gagggcttca gagggatgtg gaattccagg tggagtggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gcatcctggg aagttcctga cgctcgggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000864
VERSION     KHUT01000864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggcgtcg
       61 caagtcaggc gtgaaatccc cgggcttaac ctgggaatgg cacttgaaac tacgatgctg
      121 gagtatggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000865             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000865
VERSION     KHUT01000865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccagattta ctgggcgtaa agcgcgtgta ggcggctggt
       61 taggcgtgat gtgaaatctt ccggctcaac cggaaaactg cattgcgaac cggcctggct
      121 agagtgcagg agagggaagc ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccag tggcgaaggc ggcttcctgg cctgcaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01000866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000866
VERSION     KHUT01000866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           83 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctatt
       61 taagtcagtg gtgaaagact ccggctcaac cggagcagtg ccattgatac tggatagctt
      121 gagtattgga ggggtacatg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgaaggca ttgtactggc caataactga cgctgatgca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000867
VERSION     KHUT01000867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgt
       61 caagtcagct gtgaaatctt gaggcttaac ctcaaacggt cagctgatac tgatgtgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000868
VERSION     KHUT01000868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgtgca ggcggcttgg
       61 taagtctgat gtgaaagccc cgggcttaac ctgggaagtg cattggaaac tatcaagctt
      121 gagtactgga gaggaaggga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cccttctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000869
VERSION     KHUT01000869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatctgtctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca caattattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000870
VERSION     KHUT01000870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           82 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agcgtgtgta ggtggttgga
       61 taagttagat gtgaaatccc cgggcttaac ctgggaactg cgtctaaaac tattcagcta
      121 gagtactgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000871
VERSION     KHUT01000871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           75 g           62 t
ORIGIN      
        1 tacgaaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactat
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaactg ccattgatac tgatggtctt
      121 gagtacattt gaggtgggcg gaatgtgtca tgtagtggtg aaatacttag atatgacaca
      181 gaactccgat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000872
VERSION     KHUT01000872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           87 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggagcgca ggtggtttct
       61 taagtctgat gtgaaagccc acggcttaac cgtggagggt cattggaaac tgggaaactt
      121 gagtacagaa gaggaatgtg gaactccatg tgtagcggtg gaatgcgtag atatatggaa
      181 gaacaccagt ggcgaaggcg acattctggt ctgttactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000873
VERSION     KHUT01000873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcggat gtgaaagccc acggctcaac cgtggaagtg catccgaaac tgcatggctt
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacacctgc ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000874
VERSION     KHUT01000874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           86 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggactaa
       61 taagtcagtg gtgaaagacg gcagctcaac tgtcgaggtg ccattgatac tgttagtctt
      121 gagtatcgtt gaggtaggcg gaatggaagg tgtagcggtg aaatgcatag atatcttcca
      181 gaacaccgat agcgtaggca gcttactaag cgataactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000875
VERSION     KHUT01000875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           96 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccttt
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaagtg cattggaaac tggaaggctt
      121 gagtacggga gagggtagtg gaattccgag tgtaggggtg aaatccgtag atattcggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000876
VERSION     KHUT01000876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           78 g           57 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggaatta ttgggtttaa agggtgcgta ggcggacctg
       61 taagtcagtg gtgaaagcct gttgctcaac aacagaactg ccattgatac tgcaagtctt
      121 gagtacagtt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttgctaag ctgtaactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000877
VERSION     KHUT01000877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           90 g           44 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccaga
       61 tgtgtcaggt gtgaaagccc ggggcttaac cccggaattg cgcctgaaac tgtctggcta
      121 gagtactgga gagggtagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctacctgga cagtaactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01000878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000878
VERSION     KHUT01000878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgct gtaaaagtcc agggctcaac cctggaatgc cggtggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat agcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000879
VERSION     KHUT01000879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agcgtgcgta ggcggcgggg
       61 taagtgaggt gtgaaatccc ggagcttaac tccggaactg cactttatac tgccttgctc
      121 gagggctgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga aagctgctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000880
VERSION     KHUT01000880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           76 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggaatgg
       61 taagtccgtg gtgaaatccc cgagcttaac ttgggaactg ccatggatac tatcaatctt
      121 gaatatcatg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg cgataattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000881
VERSION     KHUT01000881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           97 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggcgtga
       61 taagccggtt gtgaaagccc cgggctcaac ctgggaacgg catccggaac tgtcaggcta
      121 gagtgcagga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000882
VERSION     KHUT01000882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggcgtc
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaattg cctttgatac tggatgtctc
      121 gagaccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000883
VERSION     KHUT01000883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           89 g           55 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagttagag gtgaaagcct ggtgctcaac accagaattg cctttgatac tgcatggctt
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000884
VERSION     KHUT01000884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           73 g           67 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggatatg
       61 taagtcagtg gtgaaatctc taggcttaac ctggaaattg ccgttgatac tatatatctt
      121 gaatgttgtg gaggtaagca gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaatacctat tgcgaaggca gcttactacg cagatattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000885             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000885
VERSION     KHUT01000885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           50 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcgggt gtgaaaactt tgggcttaac ccaaagcgtg catccgatac tgctgtgact
      121 tgagtacggt aggggagcgg ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccgg tggcgaaggc gccgctctgg gccgaaactg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000886             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000886
VERSION     KHUT01000886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           93 g           45 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatctgc
       61 caagtcctgt gtgaaagccc aaggcttaac cttggaagtg cacaggagac tggctggatt
      121 tgagttcagg agaggaaagc agaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 ggaataccag tggcgaaggc ggctttctgg actgacactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000887
VERSION     KHUT01000887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           48 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctgtc
       61 cacgtcggat gtgaaagctc ggagctcaac ttcgagatgc cattcgatac gggatagcta
      121 gaggcagcta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg gctgttctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01000888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000888
VERSION     KHUT01000888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           95 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatta ctgggcttaa agggcgcgta ggtggtggca
       61 taagtccgag gtggaagact aaggcttaac cttagaattg ccttggaaac tgtatcactt
      121 gagtcagcga ggggatggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaaggccggt ggcgaaggcg gccatctggc gctgaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000889
VERSION     KHUT01000889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggtggcttgc
       61 taagtcagac gtgaaatccc tcggcttaac cggggaactg cgtctgatac tggcgagctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgagaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000890
VERSION     KHUT01000890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcttgc
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggcgagctt
      121 gagtgcagga gaggaacgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcgttctgga ctgcaactga cactgatgaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000891
VERSION     KHUT01000891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatca ctgggcgtaa agggcgcgta ggcggttagg
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaatgg cattcgagac tgtctatcta
      121 gagtctggta gagggaagtg gaatttccgg tgtagcggtg aaatgtgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccaagactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000892             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000892
VERSION     KHUT01000892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agcgcgtgta ggcggattcg
       61 tgcgtctgtc gctgaaatcc cccggctcaa ccggggaacg ggcgcggata cgacgagtct
      121 ggaggggcat agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg atgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000893
VERSION     KHUT01000893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taggtctggt gtgaaaactc gaggctcaac ctcgagaggt cactggaaac catttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000894
VERSION     KHUT01000894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           79 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttag
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgctgagcta
      121 gaatgtagat gaagtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaactccaat tgcgaaggca gcttactaag gtacaattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000895
VERSION     KHUT01000895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          105 g           40 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgca ggcggtccgt
       61 taagtcccgt gttaaagcct ccggctcaac cggagaacgc cgcgggagac tggcgggctt
      121 gagggcggta gagggtgatg gaattcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccagt ggcgaaggcg gtcacctggg ccgtccctga cgctgagacg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000896
VERSION     KHUT01000896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 taagtctgat gtgaaatctc cgagctcaac tcggaaatgg cattggatac tattcggctc
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000897
VERSION     KHUT01000897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          101 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgttc gtgaaaacct ggggctcaac tccaggcttg cgggcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000898
VERSION     KHUT01000898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagtcggac gtgaaaatcc ggggctcaac cccggacgtg catccgaaac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000899
VERSION     KHUT01000899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           85 g           55 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggagcgta ggcggcaatt
       61 caagtcagtt gtgaaatctt cgagcttaac tcggaacggt caactgatac tgctttgcta
      121 gagtacagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgatactga cgctgaggct cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000900
VERSION     KHUT01000900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           95 g           41 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggctttg
       61 caagtcgaag gtgaaatccc ccggctcaac tggggaactg ccttcgaaac tgccaggcta
      121 gagtccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000901
VERSION     KHUT01000901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           97 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacac cgggcttaac tcggtgcttg ctttcgatac gggcagacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000902
VERSION     KHUT01000902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctcgg
       61 caagtcgggt gtgaaagccc tgggcttaac ctaggaagtg cactcgaaac tgccaagctt
      121 gagttctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000903
VERSION     KHUT01000903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           83 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcttt
       61 taagtcagtg ctgaaatact ccagcttaac tggaggggtg gcattgatac tggaagactt
      121 gagtgtagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg ctacaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000904
VERSION     KHUT01000904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggtttc
       61 taagtaaggt gtttaatccc ggggcccaac cccggacagg cactttaaac tggaagtctt
      121 gaatagtgga gaggtcagtg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccagt tgcgaaggcg gctgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01000905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000905
VERSION     KHUT01000905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttctg
       61 aaagtcagct gtgaaagccc cgggcttaac ctgggaattg cggttgaaac tacagggcta
      121 gaatctggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000906
VERSION     KHUT01000906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           83 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggattgc
       61 taagtcagtg gtgaaaggca gcggctcaac cgttgagttg ccattgatac tgtcagtctt
      121 gagtgcacta gaggtaagtg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccaat agcgaaggca acttactaga gtgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000907
VERSION     KHUT01000907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           87 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggacatt
       61 taagtcagat gtgaaatacc cgagcttaac ttgggtgctg catttgaaac tgggtgtcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000908
VERSION     KHUT01000908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtctgcc gtgaaaatcc gaggctcaac ctcggacttg cggtgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccactactga cgctgagaag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01000909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000909
VERSION     KHUT01000909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtccgg
       61 ttagtcggct gtgaaagtca agggctcaac ccttgaaggc cggtcgatac tgccggacta
      121 gagtgcggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacacctat ggcgaaggca gctcgctggg acgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000910
VERSION     KHUT01000910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtctat
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggc cattggaaac tggtaggctt
      121 gagtgcagga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000911
VERSION     KHUT01000911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           60 c          110 g           37 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctgtt
       61 caagcctgac gtgaaagccc ccggctcaac cggggagggt cgtcggggac tgggcagctt
      121 gaggtcggca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg ccggacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000912
VERSION     KHUT01000912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 taagtagggg gtgaaatcct gcggcttaac cgcagggctg ccttctaaac tgtcgagctc
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000913
VERSION     KHUT01000913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtggaag gtgaaatccc tcggcttaac cgaggaactg ccctccaaac tgctttgctt
      121 gaggccggga gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcgctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000914             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000914
VERSION     KHUT01000914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           43 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgatac tgtcgtgact
      121 agagtccagt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gctggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000915
VERSION     KHUT01000915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           85 g           54 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggttttt
       61 taagtcagag gtgaaagccc agtgctcaac actggaactg cctttgaaac tggaaaactt
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000916
VERSION     KHUT01000916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           79 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcgggcccg
       61 taagtcagtg gtgaaatctc gcagcttaac tgtgaaactg ccattgatac tgcgggtctt
      121 gagtaaattt gaagtggctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggtcactaag atttaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000917
VERSION     KHUT01000917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc acggcttaac tgtggaacgg ctgggcatac tggcggtctt
      121 gagccatcta ggggcgactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtcgctggg gatgagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000918
VERSION     KHUT01000918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtccggg gtgaaagccc acagctcaac tgtggaactg ccctggatac tggcgggctt
      121 gagtccagac gaggttggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgaaggca gctgactagg ctggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000919
VERSION     KHUT01000919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggaatg
       61 taagtcagtg gtgaaatctc cgtgcttaac atggaaactg ccattgatac tatgtttctt
      121 gaatcttccg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctgactaca ggggcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000920
VERSION     KHUT01000920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           80 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcaggg gtgaaagacg gtagctcaac tatcgcagtg cccttgatac tgaagagctt
      121 gaatggacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactatg gtttaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000921
VERSION     KHUT01000921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           89 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaagg
       61 aaagtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac ttcttttctt
      121 gagtaccgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000922
VERSION     KHUT01000922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggcgcgta ggcggcgggg
       61 taagtcgggt gtgaaatctc agggctcaac cctgaaactg cacttgatac tgctctgctt
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggcg cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000923             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000923
VERSION     KHUT01000923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           50 t
ORIGIN      
        1 gacagagggt gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggttgtttga
       61 gaagtctact gtcaaatccc agagctcaac tttggacagg cagtggagta ctcttagact
      121 agagtacggt aggggtagag ggaattcccg gtggagcggt gaaatgcaca gagatcggga
      181 agaacaccag tggcgaaagc gctctactag gccgtgactg acactgagag acgatagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01000924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000924
VERSION     KHUT01000924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcgggacag
       61 taagtcagag gtgaaagccc acagcttaac tgtggaattg cctttgatac tgctgttctt
      121 gagtcctgga gaggtcattg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga ggtgtagacg gatgactgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000925
VERSION     KHUT01000925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggctgcacgg
       61 taagtcagag gtgaaatccc ggagctcaac ttcggaattg cctccgatac tgccgtgctt
      121 gaggactgga gaggagattg gaattcacag tggagcagtg aaatgcgtag atattgtgag
      181 gaacactagt ggcgaaggcg aatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000926             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000926
VERSION     KHUT01000926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcgggt gtgaaagctc cgagctcaac tcggagaggc cacccgatac tgctgtgact
      121 tgagtccagt agaggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cagcgaaggc ggcgctctgg gctggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000927
VERSION     KHUT01000927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          107 g           38 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggtcgtg
       61 taggtacctg gtgacagtct cgggctcaac tcgagggggt ctgggtagac cgcacgactg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01000928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000928
VERSION     KHUT01000928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccctg
       61 caagtcgggt gtgaaatccc atggctcaac catggaagtg cactcgaaac tgcagggcta
      121 gagtcctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000929
VERSION     KHUT01000929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctcct
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tggggagcta
      121 gagtgtggaa gaggggggtg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000930
VERSION     KHUT01000930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggttctt
       61 taagtctgat gtgaaagccc ccggctcaac cggggaaggt cattggaaac tggagaactt
      121 gagtgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000931
VERSION     KHUT01000931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           90 g           44 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggtggtcctg
       61 aaagtcgagc gtgaaagctc ccggcccaac tgggagaggc cgttcgaaac ttcaggactt
      121 gaaggcggga gaggcaaatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaagcg atttgctggc ccgcccttga cactcaggcg cgaaagctag
      241 gggagcgacc ggg
//
LOCUS       KHUT01000932             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000932
VERSION     KHUT01000932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtccg
       61 aacgtctgtc gctgaaatcc cccggctcaa ccggggaacg ggcggagata cggcggacct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01000933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000933
VERSION     KHUT01000933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggttgat
       61 taagtctgct gtgaaagccc cgggctcaac ctgggaattg cagtggaaac tggtcagcta
      121 gagtgcggta gaggagggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatctgt ggcgaaggcg accctctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000934
VERSION     KHUT01000934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           91 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttggg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgctaaagac tgcctggctc
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccataactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000935
VERSION     KHUT01000935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcttgg
       61 taagttagag gtgaaagccc agggctcaac cctggaattg cctttaagac tgccgagcta
      121 gtaggtgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcattctaga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000936             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000936
VERSION     KHUT01000936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           47 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggcctgt
       61 taagtgttct gtgaaaaccc ggggctcaac tccgtggagt gcagaacata ctggcaggct
      121 agagaccagt agaggctagc ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacacctg tggcgaaggc ggctagctgg gctggatctg acactcatcc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000937             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000937
VERSION     KHUT01000937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           91 g           44 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgatac tgctatagct
      121 agagtccagt aggggagtgc ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcactctgg gctggtactg acgctcagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01000938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000938
VERSION     KHUT01000938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           98 g           42 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggcccgt
       61 taagttcgat gtgaaagccc cgggctcaac ctgggaatgg catcgaagac tggcaggctg
      121 gagtgcggta gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccacctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000939
VERSION     KHUT01000939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggcttct
       61 caagtcaggg gtgaaagccc agagctcaac tctggaattg cctttgagac tgagtggctt
      121 gagtacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgtaactga cgctcatgca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000940
VERSION     KHUT01000940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           88 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatcat
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tgaagatctc
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactaa cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000941
VERSION     KHUT01000941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           52 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ccaggaagtg cattggaaac tggcagactt
      121 gaatacggga gagggcagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000942
VERSION     KHUT01000942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggccctg
       61 taagtccgct gtgaaagtca agggctcaac ccttggatgc cggtggatac tgcagggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000943             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000943
VERSION     KHUT01000943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 aaagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac ttctatggct
      121 agagtttggc aggggaacac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgttctgg gccaagactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000944
VERSION     KHUT01000944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacgtaggga gcgagcgtta cccggaatca ctgggcgtaa agtgcgcgta ggcggcttgg
       61 aaagtctctt ttgaaagctc ccggcttaac tgggagaggt gaggagaaac tacctggctt
      121 gagggcagta gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctttctggg ctgtacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000945
VERSION     KHUT01000945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgaa
       61 caagtcaggt gtgaaagccc tcggctcaac cgaggaactg cgcttgaaac tgtttggctc
      121 gagtcaacta gaggtgcacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgcactggg gttggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000946
VERSION     KHUT01000946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacggaggga gcgaacgttg ttcggattta ctgggcgtaa agggtgtgta ggcggtcttg
       61 taagtcaggg gtgaaatctc tcggctcaac cgagaaactg cctttgagac tgcaggactt
      121 gagggcgtga gaggagactg gaattcctag tgtagcggtg aaatgcatag atattaggag
      181 gaacaccagt ggcgaaggcg agtctctggc acgcatctga cgctgaaaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000947
VERSION     KHUT01000947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgagtgta ggtggccgca
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaatgg cattcgatac tgtgtggcta
      121 gagtctgata gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gcttcctgga tcaagactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000948
VERSION     KHUT01000948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttttc
       61 caagttgggt gtgaaagcct tgggcttaac ccaagaaatg cactcagtac tggaagacta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000949
VERSION     KHUT01000949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggattta ctgggcgtaa agcgtacgta ggcggatcgt
       61 taagtgaggg gtgaaatccc ggggctcaac ctcggaactg cctttcatac tggcgatctt
      121 gagtccggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000950
VERSION     KHUT01000950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          102 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccctc
       61 cttgtcgcgt gtgaaagccc cgggctcaac ctgggaattg cgcgcgaaac gggggggctt
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000951
VERSION     KHUT01000951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           99 g           45 t
ORIGIN      
        1 tacgaaggtg gcgagcgttg ttcggattta ctgggcgtaa agagcacgta ggcggttggg
       61 taagcctctt gggaaatctc tcggcctaac cgagaaagtt cgaggggaac tacttagcta
      121 gagggcggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtacctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000952
VERSION     KHUT01000952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcattg
       61 ttagtcactt gtgaaatctc cgggcttaac tcggaacggc caagtgatac tgcagtgcta
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacaccaga ggtgaagacg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000953
VERSION     KHUT01000953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcggg
       61 taagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaagac tgctcgactg
      121 gagactggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctaga ccagatctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000954
VERSION     KHUT01000954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           89 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggatctt
       61 taagtcaggg gtgaaatgcc ggagctcaac ttcggaactg cctttgatac tggagatctt
      121 gagttcgaga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000955
VERSION     KHUT01000955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           91 g           44 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcggtg
       61 taagtctgct gttaaagagt gaggctcaac ctcataaaag cagtggaaac tacacagcta
      121 gagtgcggta ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000956
VERSION     KHUT01000956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           48 c           99 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggtggctgtt
       61 caagtcgggt gtgaaatccc aaggctcaac cttgggtctg cattcgatac tgggtggctt
      121 gagtctggta gggggacctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gggtcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000957
VERSION     KHUT01000957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcctgg
       61 taagtagggg gtgaaatctc gaggctcaac ttcgaggctg cctcctaaac tgtcaggctt
      121 gagcagagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ctcttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000958
VERSION     KHUT01000958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          100 g           39 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ctgggcgtaa agcgcgcgta ggcggctcgt
       61 taagtcggat gtgaaaaccc ggggctcaac cccgggcctg cacccgatac tggcgggctg
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000959
VERSION     KHUT01000959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtccga
       61 taagtcggct gtgaaagtcc agggctcaac tctggaatgc cggccgatac tgtcggacta
      121 gagtccggaa gaggtgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcactggg acggcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000960
VERSION     KHUT01000960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           63 c          113 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctcgc
       61 tttgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggcgggctt
      121 gaggccggca ggggcaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcctgctggg ccggacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000961
VERSION     KHUT01000961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggccttg
       61 ttagtcggac gtgaaatccc caagcttaac ttgggaactg cgtccgatac tgcaaggctt
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgaaactga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000962
VERSION     KHUT01000962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttta
       61 taagtcagtg gtgaaagacg tcagcttaac tgtcgcagtg ccattgatac tgtagaactt
      121 gagtatagtt gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaaccccaat agcgtaggca gcttactaaa ctataactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01000963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000963
VERSION     KHUT01000963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           99 g           42 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcgtc
       61 taagtcactt gtgaaatctc cgggctcaac ccggagcggc caagtgatac tgggcgacta
      121 gagtgcggaa ggggcaaccg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000964             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000964
VERSION     KHUT01000964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggttttg
       61 caagccagag gtgaaatgcc tgagctcaac ttaggaattg cctttgggac tgcgaggctt
      121 tgaggactgg agaggtgagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccag tggcgaaggc ggctcactgg ccagtttctg acgctgcagc gcgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01000965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000965
VERSION     KHUT01000965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggcgcag
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaattg cgctctaaac tactgtgctt
      121 gaggaagcta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01000966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000966
VERSION     KHUT01000966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggttgag
       61 taagtcacct gtgaaatctc ccggctcaac tgggagcttg caggcgaaac tgcttgactg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000967
VERSION     KHUT01000967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggctttc
       61 taagtcggat gtgaaaactc tgggctcaac ccagagcctg catctgaaac tggagagcta
      121 gggttctgga ggggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cagataccga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000968
VERSION     KHUT01000968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           78 g           59 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaagccc caagcttaac ttgggaactg ccattgatac tgatggtctt
      121 gagtacattt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000969             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000969
VERSION     KHUT01000969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          105 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggcccgt
       61 taagtctctt ggtgaaaggc cggggctcaa ccccgagcaa gccggggata ctggggggct
      121 ggagacaggt agaggccagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggctggctgg acctgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01000970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000970
VERSION     KHUT01000970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggctatg
       61 taagctagat gtgaaagccc cgggcttaac ctgggaattg catttagaac tgcatggcta
      121 gagtacagaa gagggcagtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg actgcctggt ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000971             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000971
VERSION     KHUT01000971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggacct
       61 gcaagtcgga agtgaaatcc cggggctcaa ctccggggct gctttcgaaa ctgcggacct
      121 agagaccagt aggggttggt ggaattccca gtgtagcggt ggaatgcgta gatattggga
      181 agaacacctg tggcgaaggc ggccaactgg gctggatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01000972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000972
VERSION     KHUT01000972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           97 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggtttgt
       61 ttagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgcttgatac aggcagactt
      121 gagtgtggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000973
VERSION     KHUT01000973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          100 g           42 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggtggcatgg
       61 caagtaaggt gtgaaatctc cctgctcaac ggggagggag cgctttagac tgccaagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000974
VERSION     KHUT01000974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtag agcgtgcgca ggcggcttcg
       61 caagtcaggt gtgaaatccc cgggcttaac ctgggaatgg cgcttgaaac tgcggggcta
      121 gagtctgcca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg gtaagactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000975
VERSION     KHUT01000975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c          101 g           40 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgca ggcggattgg
       61 taagtgggtc gtgaaagcct ccggctaaac tgggggagga cgatacagac tgccagtcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01000976             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000976
VERSION     KHUT01000976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           91 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcggcggtg
       61 taagttggaa gtgaaatccc atcggctcaa ccgtggaatt gctcccagta ctgcatcgct
      121 cgagggggat aggggtttgc ggaactaccg gtggagcggt gaaatgtgtt gatatcggtg
      181 ggaacgccag tggcgaaagc ggcaaacttg acccttcctg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000977
VERSION     KHUT01000977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggttctt
       61 tcagttcaaa gtgacagtcg tcggctcaac tgacgaagga ctgggaagac gggagaactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaac agcgaaggca gtgtcctggg aagtctctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01000978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000978
VERSION     KHUT01000978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccacg
       61 caggtccgct gtgaaaactc gaggctcaac ctcgagatgt cggtggaaac cgcgtggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000979
VERSION     KHUT01000979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttcga
       61 caagtgggat gtgaaagccc tgggctcaac ctgggaactg cattccaaac tatcgggcta
      121 gagtgtggta gagggaggta gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaataccagt ggcgaaggcg gcctcctgga ccaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000980
VERSION     KHUT01000980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggcttcc
       61 taagtcggtt gtgaaatccc tgggctcaac ctgggaattg cagtcgatac tgggaagctc
      121 gagttcggta gaggacggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcgat ggcgaaagca accgtctggg cctgaactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000981
VERSION     KHUT01000981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaattg cgatggatac tggcgagcta
      121 gagtacggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccatctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000982
VERSION     KHUT01000982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           84 g           52 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatcgg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgccagtctt
      121 gagccagcta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg gctgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000983
VERSION     KHUT01000983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           38 c           74 g           65 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttaa
       61 taagtcagtg gtgaaagcct atcgcttaac gatagaacgg ccattgatac tgttagactt
      121 gaattagtgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactatc actatattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000984
VERSION     KHUT01000984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           35 c           76 g           67 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctcat
       61 taagtcagtg tttaaagtca acagctcaac tgttggaatg gcattgatac tgatgagctt
      121 gagtaaagtt gaggtagaca gaattgatgg tgtagcggtg aaatgcatag atatcatcaa
      181 gaataccgat tgcgaaggca gtttactaag ctttaactga cactgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01000985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000985
VERSION     KHUT01000985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggatttg
       61 taagttgggg gtgaaatccc gaggctcaac ctcggaactg cctccaaaac tacaagtctg
      121 gacctaagta gaggcaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg acttgctgga cttatggtga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000986
VERSION     KHUT01000986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttt
       61 taagttccgt gtgaaatccc tcggctcaac tggggaactg cacgggaaac tggaaggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000987
VERSION     KHUT01000987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           87 g           49 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttag
       61 taagtctcct gttaaagatc agggcttaac cctgagtcgg cagtagaaac tactgagctt
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaat ggcgaaagca ctctgctggg ccgaaactga cactcagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01000988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000988
VERSION     KHUT01000988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          106 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgt
       61 taagggtgcg gtgaaagccc ggggcttaac cccgggtcgg ccgtgccgac tgatgagctc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000989
VERSION     KHUT01000989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggttttt
       61 caagtctgct gtcaaagcgt ggagctcaac tccataacgg cagtggaaac tgggagacta
      121 gagtgcgata ggggtaacgg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccagc ggcgaaagcg cgttactggg tctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01000990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000990
VERSION     KHUT01000990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgtatgca ggcggatcgt
       61 taagtgaggg gtgaaatccc gaggctcaac ctcggaactg cctttcatac tgacgatctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000991
VERSION     KHUT01000991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           96 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctttt
       61 caagtcgggc gtgaaatctc caggcttaac ctggaagggt cgttcgatac tgttaagcta
      121 gagtgtggaa gaggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01000992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000992
VERSION     KHUT01000992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatctt
       61 taagtcgggg gtgaaatccc ggggctcaac cccggaactg ccttcgatac tgaggatctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000993
VERSION     KHUT01000993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggttact
       61 caagtcggat gtgaaagccc tcggcttaac tgaggaagtg cacccgaaac tgagtagcta
      121 gagtactaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01000994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000994
VERSION     KHUT01000994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           99 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggcttgt
       61 cgcgtctgct gtgaaaaccc ggggcttaac cccgggcttg cagtgggtac gggcaggcta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccattactga cgctgagaag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01000995             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000995
VERSION     KHUT01000995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           49 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaatt
       61 caagtcatct gtgaaatctc cgggcttaac ccggaacggt caggtgatac tgttttgcta
      121 gagtgcggaa ggggcaatcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccaga ggtgaagacg ggttgctggg ccgacactga cgctgacagc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01000996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000996
VERSION     KHUT01000996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 gacgtagggg gccagcgttg ttcggaatta ctgggtgtaa agggttcgta ggcggtgcgg
       61 caagttggga gtgaaatctc tgggctcaac ccagaggctg cttccaaaac tgctgtgctt
      121 gagtgtggga gaggcgcgtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaagcg gcgcgctgga ccactactga cgctgaagaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01000997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000997
VERSION     KHUT01000997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggttctc
       61 taagttccgt gtgaaatctc ccggctcaac tgggagggtg cgcggaaaac tggagggctt
      121 gagtatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gctttctgga ccatcactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01000998             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000998
VERSION     KHUT01000998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           48 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgg
       61 taagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac tgctgtgact
      121 ggagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggtactg acactgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01000999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01000999
VERSION     KHUT01000999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaatgg catttgatac tggctgacta
      121 gagtgtggta gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccacctgga ccaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001000
VERSION     KHUT01001000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcattg
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tgcagtgctt
      121 gagtaccgga gagggtgacg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gtcacctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001001
VERSION     KHUT01001001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           75 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggggtgg
       61 taagtcagtg gtgaaatctc caggcttaac ttggaaactg ccattgatac tatcattctt
      121 gaataccgtg gagatgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgttaca cgaatattga cactcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001002
VERSION     KHUT01001002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           98 g           42 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcccgg
       61 aaagtcggtt gtgaaaaccc ggggctcaac cccgggcctg catccgatac ttccgggctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01001003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001003
VERSION     KHUT01001003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 gatgtctgat gtgaaagccc cgggctcaac ctgggaattg cattggaaac tagcgggctg
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001004
VERSION     KHUT01001004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtctgat gtgaaatcac gtggctcaac cacgtgcggt cattggaaac tggagggctt
      121 gagtgcagaa gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001005
VERSION     KHUT01001005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           89 g           44 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggatcag
       61 caagtcaggt gtgaaatctc gaagctcaac ttcgaaactg cgcctgaaac tgctagtcta
      121 gaatgtcgga gggggcaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctgcctgga cgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001006
VERSION     KHUT01001006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaagg
       61 atagtcagat gtgaaatccc tgggctcaac ccaggaactg catttgaaac tccctttctt
      121 gagtgctgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001007             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001007
VERSION     KHUT01001007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 caagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcgaac tgctatggct
      121 tgagcactgt agaggcaggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcctgctgg gcagtggctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001008
VERSION     KHUT01001008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 cacgaaccgg acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggtgcgg
       61 taagtgcggt gtgaaatccc tcggctcaac cgaggaattg cgctgcatac tgccgtgctg
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001009
VERSION     KHUT01001009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggattct
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tggggatctt
      121 gagtccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggaactga cgctgaggca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001010
VERSION     KHUT01001010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           49 t
ORIGIN      
        1 tacgtagggt gcaaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggtccat
       61 taagtccttt gtgaaagccc ccggctcaac cggggaattg caggggatac tgttgggctt
      121 gagtgcagga ggggagagcg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg gctctctggt ctgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001011
VERSION     KHUT01001011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggctatg
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tatatagctt
      121 gaattatgtt gaggttggcg gaatataaca tgtagcggtg aaatgcatag atatgttata
      181 gaacaccgat tgcgaaggca gctggctaaa catatattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001012
VERSION     KHUT01001012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggctgg
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tactggtctt
      121 gaatattgtt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactggg caatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001013
VERSION     KHUT01001013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatact
       61 taagttaggg gtgaaatccc ggggctcaac cccggaactg cctttaatac tgggtatctc
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001014
VERSION     KHUT01001014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           41 c           76 g           56 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtctact gttaaaaatt gaagcttaac ttcaaagatg ccgtagaaac ttttaaactc
      121 gaggatggta ggggtagagg gaatttccag tggagcggtg aaatgcgtag atcttggaaa
      181 gaccaccaat ggcgaaggca ctctactggg ccattactga cactaagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01001015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001015
VERSION     KHUT01001015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttgt
       61 taagttggat gtgaaagccc cgggctcaac ctgggaactg catccaaaac tggcaagcta
      121 gagtatggca gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg accacctggg ctaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001016
VERSION     KHUT01001016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           84 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggccgcgcgg
       61 gcagtcaaat gtgtaattca ggggctcaac ccctgcactg cgcttgatac ttccgcgctc
      121 gagctttgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacacttag ggcgaaggca agactctgga caaatgctga cgctcatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001017
VERSION     KHUT01001017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctttg
       61 caagtcaggt gtgaaagccc ccggctcaac cggggaattg cgcttgaaac tgcagggctc
      121 gagtcagcta gaggtgcacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgcactggg gctggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001018
VERSION     KHUT01001018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtccgca ggcggtcaag
       61 taagttcgag gtgacagtgc gtggcttaac cgcgcaaggt ccttgaaaac tgctcgactt
      121 gagggcttga gagggacccg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaaa ggcgcaggca gggtcctggc aagccactga cgctcatgga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001019
VERSION     KHUT01001019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           53 c          107 g           42 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtctta
       61 taagggtgtg gtgaaagccc ggggctcaac cccgggtcgg ccgtgccgac tgtaagacta
      121 gagtgctgta ggggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggag
      181 gaagaccggt ggcgaaggcg gcctgctggg cagttactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001020
VERSION     KHUT01001020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          101 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctgtt
       61 caagtcggta gtgaaaactc aaggcttaac cttgggcttg ctgtcgagac tgggcggctg
      121 gagtctggta ggggggtctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggaccctggg ccagtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001021
VERSION     KHUT01001021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatccc gcggctcaac cgtggaactg cctcagatac tgacggcctc
      121 gagggagata ggggcgagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaactccggt ggcgaaggcg gctcgctgga tcttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001022
VERSION     KHUT01001022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          103 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctttt
       61 caagcgtgcg gtgaaagctc agggctcaac cctgagtcgg ccgtgcgaac tggaaggctg
      121 gagcacggta gaggcacatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgtgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001023
VERSION     KHUT01001023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggacttg
       61 taagttgggg gtgaaatccc gaggctcaac ctcggaactg cctccaaaac tacaagtctg
      121 gagcaagata gaggcaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg acttgctgga tctttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001024
VERSION     KHUT01001024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c           99 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgaat gtgaaatccc gtggctcaac tgcgggtctg cattcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001025             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001025
VERSION     KHUT01001025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           60 c           94 g           38 t
ORIGIN      
        1 gacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcccg cgtgaaatcc cccggctcaa ccggggaacg gccgggcata ctggcggtct
      121 tgggccagct aggggtcatc ggaacaaacg gtggagcggt gaaatgcgta gatatcgttt
      181 ggaacgccga cggtgaaaac agatgactgg ggctgtgccg acgctgagac acgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001026
VERSION     KHUT01001026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           93 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggtgggacag
       61 taagtcaggg gtgaaagccc gcggcctaac cgcggaactg cctttgatac tgctgttctt
      121 gagtcccgga gaggttgttg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga ggcgtaggcg aacaactgga cgggtactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001027
VERSION     KHUT01001027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgga
       61 tcagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgtccgactc
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001028
VERSION     KHUT01001028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          105 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggttggg
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccatgcgaac tgcccgactg
      121 gagcacggta gaggccaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttggctggg ccgtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001029
VERSION     KHUT01001029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           88 g           49 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggattta ctgggcgtaa agagcacgta ggcggttgat
       61 taagttggat gtgaaagccc agggcttaac cttggaacgg catacaaaac tgatgaactc
      121 gagtaaggga gaggagagtg gaatttccgg tgtagcggtg aaatgcacag atatcggaag
      181 gaacaccagt ggcgaaggcg actctctggc ctattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001030
VERSION     KHUT01001030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaat
       61 taagtcacac gtgaaatccc tcggctcaac cggggaactg cgtgtgagac tggatggctt
      121 gagtttggga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccaatactga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001031
VERSION     KHUT01001031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           82 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 aaagtcggat gtgaaatctc caagctcaac ttggaaactg cattggaaac taccttgcta
      121 gaggatttga ggggggattg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg aatccctgga aaatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001032
VERSION     KHUT01001032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           79 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgatgggctt
      121 gaatgaacct gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaaa gtttaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001033
VERSION     KHUT01001033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           79 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtccgtg gtgaaatccc caagcttaac ttgggaactg ccgtggatac tatcaatctt
      121 gaatatcgtt gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcggaggca gcttactaag cgatcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001034
VERSION     KHUT01001034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 tgtgtctgtc gtgaaagccc cgggcttaac ctgggaatgg cgatggaaac tggcgagctt
      121 gagtgtggca gaggggggtg gaactcctgg tgtagcagtg aaatgcgtag agatcaggaa
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001035
VERSION     KHUT01001035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           81 g           56 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggatgtt
       61 taagtgagtt gttaaatcgt gtggctcaac catatcgatg caattcatac tgggcatcta
      121 gaggacggca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001036
VERSION     KHUT01001036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggatagt
       61 taagtccggt gtgaaagatc agggctcaac cctgagagtg catcggaaac tggttatctt
      121 gaggacagga gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001037
VERSION     KHUT01001037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          105 g           42 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctcga
       61 taagactggc gtgaaagccc ccggctcaac cggggagggt cgttggtgac tgtcgagctt
      121 gaagacagca ggggcaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcctgctggg ctgatcttga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001038
VERSION     KHUT01001038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggcgtg
       61 caagtcaggc gtgaaatccc tgggctcaac ccaggaattg cacttgaaac tgcacgtctt
      121 gagtatcgga gaggaaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001039
VERSION     KHUT01001039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggctcgg
       61 taagtctgct gtgaaagtcc aaggctcaac cttggaatgc cggtggatac tgtcgagctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001040
VERSION     KHUT01001040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           92 g           48 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatctga
       61 caagtctggt gtgaaagccc acatgttaac tgtggaagtg cattagaaac tgttggattt
      121 gagttcagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001041
VERSION     KHUT01001041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           97 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatatc
       61 ttagtcagac gtgaaattcc tgggcttaac ctgggggctg cgtttgatac ggggtatcta
      121 gagtggggaa gagggttgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcaacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001042
VERSION     KHUT01001042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggggatg
       61 taagtcgggc gtgaaatccc caggctcaac ctgggaactg cactcgaaac tgcgtttctt
      121 gagtatcgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001043
VERSION     KHUT01001043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c           99 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaaacgc gaggcttaac ctcgcgcctg cagtgggtac gggcagacta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01001044             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001044
VERSION     KHUT01001044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          104 g           39 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggattct
       61 gtaagtcgga agtgaaattc cggggctcaa ctccggaact gcttccgaga ctgcgagtct
      121 ggggaccggt agaggcgggt ggaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacaccgg tggcgaaggc ggcccgctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001045
VERSION     KHUT01001045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggcgtt
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tattcgtctt
      121 gaatatcgtt gaggtaggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gcttgctaaa cgatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001046
VERSION     KHUT01001046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggtttta
       61 caggtccgct gtgaaaactc gaggctcaac ctcgagctgt cggtggaaac cgtaaggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001047
VERSION     KHUT01001047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccga
       61 taagtccgct gtaaaagtca acggctcaac tgttgaaagc cggtggatac tgtcgggcta
      121 gagtacggaa gaggcaagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gcttgctggg acgttactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001048
VERSION     KHUT01001048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggcctga
       61 tcagtcgggg gtgaaagcct gtggctcaac cacagaatgg ccttcgatac tgtcaggctt
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001049
VERSION     KHUT01001049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctgcg gtgaaatctc ccggctcaac tgggagtctg ccgtggaaac tgccgtgctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001050
VERSION     KHUT01001050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           76 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattag
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tattagtctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgagcattga cactcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001051
VERSION     KHUT01001051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcgag
       61 taagtccgtg gtgaaatcct gcagcttaac tgcagaactg ccatggatac tatttgtctt
      121 gaatatcgtg gagatcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gctggttacg cgaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001052
VERSION     KHUT01001052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           85 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggaggctggg
       61 aaagtcggat gtgaaatctc accgcttaac ggtgaaactg cattcgatac tactcggcta
      121 gaggatcgga ggggagagca gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaataccaac agcgtaggca gctctctgga agattcctga ctctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001053
VERSION     KHUT01001053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           90 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtgggtttg
       61 caagttcgat gtgaaagccc cgggcttaac ccgggaatgg cattggatac tgcagatctg
      121 gaggatcgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001054
VERSION     KHUT01001054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgca ggcggtgtga
       61 tgcgtcgctg ctgaaagctc tcggctcaac tgagagaggc gtggcgatac gatcatactc
      121 gagtctctga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc agagcactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac cgg
//
LOCUS       KHUT01001055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001055
VERSION     KHUT01001055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccaag
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccgaaac tatttggctt
      121 gagtaccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001056
VERSION     KHUT01001056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           48 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cacgtcgggc gtgaaaactc agggcttaac cctgagcgtg cgttcgatac gggcagacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg catgacctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01001057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001057
VERSION     KHUT01001057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgact gtgaaaaccc gaggcttaac ctcgggcctg cagtcgatac gggcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001058             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001058
VERSION     KHUT01001058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           45 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcgggt gtgaaatttc agggctcaac cctgaaacgc cacccgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcat
      241 gggtagcgaa cagg
//
LOCUS       KHUT01001059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001059
VERSION     KHUT01001059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          102 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcgcat
       61 ttagtcaggc gtgaaattcc tgggctcaac ctggggactg cgcttgagac gggtgtgcta
      121 gagggcagga gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggc ctgtgactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001060
VERSION     KHUT01001060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtccggg gtgaaagccc gttgctcaac aacggaactg ccctggatac tggggagctt
      121 gagtacagac gaggttggcg gaatggaccg agtagcggtg aaatgcatag atacggtcca
      181 gaaccccgat tgcgaaggca gctgactagg ctgatactga cgctgaggca cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001061
VERSION     KHUT01001061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttgg
       61 caagtttcgt gtgaaatctt cgggctcaac tcgaagtctg cacggaaaac tgccgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001062
VERSION     KHUT01001062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           88 g           47 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaagccc tcggctcaac cgaggaattg cgcccaaaac ttccgtgctt
      121 gaggaagata gaggtgagcg gaactcaggg tggagcggtg aaatgcgttg atatcctgag
      181 gaacaccggt ggcgaaagcg gctcactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001063
VERSION     KHUT01001063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgttc
       61 caagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggaatgctc
      121 gagcgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtagacg gctttctgga ccattgctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001064
VERSION     KHUT01001064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggtggttcat
       61 tgcgtcgtgc gtgaaagacc ccggctcaac tggggagggt cgtgcgaaac gggtgaacta
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cactcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01001065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001065
VERSION     KHUT01001065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgattcga
       61 aaagtcaact gtgaaagccc agggcctaac cctggaagtg cagctgatac tgttgaattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001066
VERSION     KHUT01001066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          107 g           40 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcgcga
       61 taagaccggc gtgaaagccc ccggctcaac cggggagggt cgttggtgac tgtcgcgctt
      121 gaaggcagca ggggtgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gctcactggg ctgaccttga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001067
VERSION     KHUT01001067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggattgg
       61 tcagttagag gtgaaatccc gaagctcaac ttcggaactg cctttaatac tgccagtcta
      121 gagtccggaa gaggtaagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001068
VERSION     KHUT01001068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacggaggga gctagcgtta ttcggaatta ctgggcgtaa agcgtacgta ggcggctttg
       61 caagttagag gtgaaagcct ggagctcaac tccagaactg cctttaaaac tgcatcgctt
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001069
VERSION     KHUT01001069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 cacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtgggt
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaaaac tggccgactg
      121 gagagtggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccacatctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001070
VERSION     KHUT01001070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggcccga
       61 aaagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac tgtcgggctt
      121 gagtacggga gagggttgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcagcctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001071
VERSION     KHUT01001071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           96 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacca ggtgcttaac acctggcttg ctttcgatac gggcagacta
      121 gaggtattca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg aatgacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001072
VERSION     KHUT01001072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           71 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttta
       61 taagtcagtg gtgaaatccg gtagctcaac tatcgaactg ccattgatac tgtaggactt
      121 gaattattgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactaac aatacattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001073
VERSION     KHUT01001073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtccgca gtgaaatctc tgggctcaac tcagagtctg ctgtggaaac tgccgtgctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001074
VERSION     KHUT01001074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           89 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttaca
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgagac tgcgtagcta
      121 gggtacggta gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcttcctgga ccagtaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001075
VERSION     KHUT01001075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           89 g           46 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctga
       61 caggtcactt gtgaaatctc cgagcttaac tcggaacggc caagtgaaac caacaggcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001076
VERSION     KHUT01001076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           83 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggttaga
       61 taagtcagtg gtgaaaggtt gccgcttaac ggtagaatgg ccattgatac tgtttaactt
      121 gaatcaggat gaggttagcg gaatgagaca tgtagcggtg aaatgcatag atatgtctta
      181 gaacaccaat tgcgaaggca gctagctaga cctagattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001077
VERSION     KHUT01001077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           86 g           50 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttat
       61 taagtctact gttaaagatc agggcttaac cctgagccgg cagtggaaac taataagctt
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaat ggcgaaagca ctctgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001078
VERSION     KHUT01001078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggtcatg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgagac tgcatggcta
      121 ggatgcggaa gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcttcctggt ccagcatcga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001079
VERSION     KHUT01001079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacgtaggca gctagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtttgc
       61 caagtccgtt gtgaaatctc ccggctcaac tgggagggtg cggcggaaac tggcaggcta
      121 gagtgcggaa tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgaagacg gctctctgga ccgctactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001080
VERSION     KHUT01001080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ttgggcgtaa agcgcgtgca ggcgggcgag
       61 caagtctgat gtgaaagccc cgggctcaac ctgggaagtg cattggatac tgttcgtctt
      121 gagttctgga gaggatgggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaataccagt ggcgaaggcg cccatctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001081
VERSION     KHUT01001081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           98 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcggt
       61 taagtcagtt gtgaaagccc ggggctcaac cccggagggt cgactgatac tgattgacta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001082
VERSION     KHUT01001082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggctgcgcag
       61 aaagtcaggt gtgaaatcca ggagctcaac ttctgaattg cacccgatac tcctgtgcta
      121 gaggattgga gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacactagt ggcgaaggct gggctctgga caatatctga cgctgaggca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001083
VERSION     KHUT01001083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcagat gtgaaagccc tcggcttaac cgaggaagtg cgtctgaaac tgctgagctt
      121 gagtgtcgga gaggatggcg gaattccgca agtagaggtg aaattcgtag atatgcggag
      181 gaacaccggt ggcgaaggcg gccatctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001084
VERSION     KHUT01001084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctctt
       61 caagtcagac gtgaaagcct caggctcaac ctgagaacgg cgtttgatac tgtcgagcta
      121 gagtgtggaa gaggctattg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg gatagctggg ccaacactga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001085
VERSION     KHUT01001085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          104 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagcgtgcg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tggtggactg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gcctgctggg cagttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001086
VERSION     KHUT01001086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           86 g           56 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggcgtgt
       61 caagtcagtt gtgaaatctt aaggcttaac cttaagcggt caactgatac tgtcatgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001087             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001087
VERSION     KHUT01001087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           97 g           47 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattca ctgggcgtaa agggcgcgca ggcggttacg
       61 taagtccggc gtgaaatccc atggcttaac tgtggaactg cgtcggatac tgcgttgact
      121 tgaggatggt agaggccatt agaactagcg gtgtagcggt gaaatgcgca gatatcgcta
      181 ggaatgccgg tggcgaaggc ggatggctgg gccactcctg acgctgaggc gcgaaagcta
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01001088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001088
VERSION     KHUT01001088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccaga
       61 caagtcaggg gtgaaagcct gtggctcaac cacagaattg cctttgatac tgtttggctc
      121 gagaccggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg gccaactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001089             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001089
VERSION     KHUT01001089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gtaagtctga agtgaaattt caccgctcaa cggtgaagct gcttcggaga ctgcggatct
      121 ggagaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001090
VERSION     KHUT01001090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcgaacgtta atcggaatca ctgggcgtaa agcgcgcgta ggcggtctgt
       61 taagtgggat gtgaaatccc cgggcttaac ctgggaattg catttcaaac tggccgacta
      121 gagtacggta gagggaggta gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaataccagt ggcgaaggcg gcctcctgga tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001091
VERSION     KHUT01001091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           92 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcttc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tgggaggcta
      121 gagtactgga tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctctctgga cagtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001092
VERSION     KHUT01001092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggccttt
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tggaaggctt
      121 gagtacagaa gagaagagtg gaattccatg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctttggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001093
VERSION     KHUT01001093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcatgg
       61 taagtcgggt gtgaaatccc ggggctcaac cccggaactg cactcgatac tgccgtgctc
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccggt ggcgaaggct agtctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001094
VERSION     KHUT01001094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtacgca ggcggctgcg
       61 caagacaggt gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcgcagcta
      121 gagtttgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg acctgactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001095
VERSION     KHUT01001095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           86 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttcag
       61 taagttggat gtgaaatccc cgggctcaac ctgggaactg catccaaaac tgctgaacta
      121 gagtacggta gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg accacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001096
VERSION     KHUT01001096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtcggtt gtgaaaattg gaggctcaac ctccagacgt cgatcgaaac catctggcta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001097
VERSION     KHUT01001097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           87 g           53 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agcgcgcgta ggcgggtttt
       61 caagtcggtt gtgaaatccc tcagctcaac tgaggaactg catccgatac tgtctgtctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg actctctggc tcgtatctga cgctgaggcg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001098
VERSION     KHUT01001098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           74 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggatatg
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaattg ccattgatac tatatatctt
      121 gaatgctgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacacctat tgcgaaggca gcttactacg cagatattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001099
VERSION     KHUT01001099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgaat gtgaaaaccc ggggctcaac cccgggcctg cattcgatac gggcagacta
      121 gagttcggta ggggagtctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001100             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001100
VERSION     KHUT01001100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c          101 g           47 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctcca
       61 tatgtcggcg gtgaaagcat acagctcaac tgtattgagc cgcggaaact gtggggcttg
      121 aggctgggag gggctggtgg aattccctgt gtagcggtga aatgcgtaga gatgggggag
      181 aacacttgtg gcgaaggcgg ccagctggac cagttctgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001101
VERSION     KHUT01001101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c          104 g           44 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgcatgca ggcggctcat
       61 tacgcacggc gtgaaagccc ccggctcaac cggggagggt cgtcgtgtac gggtgagctt
      121 gagggtatca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccagctggg atacacctga cgctgagatg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001102
VERSION     KHUT01001102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgt
       61 ttagtcgggt gtgaaatccc ggggctcaac cccggaactg cattcgatac tggcaggcta
      121 gagaccggaa gaggtgggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001103
VERSION     KHUT01001103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           94 g           40 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgct gtgaaagtca aaggctcaac ctttgaatgt cagcggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccaac ggcgaaggca gctcgctggg acggaactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001104
VERSION     KHUT01001104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           82 g           53 t
ORIGIN      
        1 gacgggggat gcaagtgtta tccggaataa ttgggcgtaa agcgtctgta ggtggtttac
       61 caagtctgtt gttaaaactc aaggcttaac cttgatcagg caacagaaac tagtaggctt
      121 gagtacggta ggggcagagg gaattctcgg tgtagtggtg aaatgcgtag atatcgagaa
      181 gaacaccaat agcgaaagca ctctgctggg ccgaaactga cattgagaga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01001105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001105
VERSION     KHUT01001105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          102 g           39 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcccgc
       61 caagtcactt gtgaaatctc cgggcttaac ccggagcggc caagtgatac tgacgggctg
      121 gagtgcggaa ggggccaccg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggtggctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001106
VERSION     KHUT01001106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttttg
       61 taagccagat gtgaaatccc cgggctcaac ctgggaatgg catttgggac tgcaaggcta
      121 gagtgtggca gaggagactg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca ggtctctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001107
VERSION     KHUT01001107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatgct gcggctcaac cgtagaactg ccttagatac tgacggcctc
      121 gaggaaggta ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaagcg gcgtgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001108
VERSION     KHUT01001108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           63 c          110 g           33 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggcgctc
       61 caagcccgac gtgaaagccc ccggctcaac cggggagggt cgtcggggac tggggcgctt
      121 gagggcggta ggggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt agcgaaggcg gccggctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001109
VERSION     KHUT01001109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatca ctgggcgtaa agcgtgcgta ggcggttggt
       61 taagtctgct gtgaaagccc tgggctcaac ctgggaactg cagtggatac tggccagcta
      121 gagtgtgata gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccatctgga tcaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001110             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001110
VERSION     KHUT01001110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           54 t
ORIGIN      
        1 tacgtagggg gctagcgtta tccggattta ctgggcgtaa agggtgcgta ggtggtttct
       61 taagtcagga gtgaaaggct acggctcaac cgtagtaagc tcttgaaact gggaaacttg
      121 agtgcaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagta gcgaaggcgg ctttctggac tgtaactgac actgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001111
VERSION     KHUT01001111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcggac gtgaaatccc ccggctcaac cggggaactg cgtccgatac tggtgggctc
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcatcctgga ccggcattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001112
VERSION     KHUT01001112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcacgta ggcggcctca
       61 taagtccgtt gtgaaagtca aaggctcaac ctttgaaagc cgatggatac tgtgaggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acggtactga cgctaaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001113
VERSION     KHUT01001113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacagaggtg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctgag
       61 taagtcctgg gtgaaatccc tcggctcaac cggggaactg ccttggaaac tactcggctc
      121 gaggccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001114             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001114
VERSION     KHUT01001114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcatt
       61 taagttcggg gtgaaaggtt cagcgctcaa cgcgaacagt gccttgaata ctgggtgact
      121 tgagttcggg agagggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctgcctgg cctagaactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001115
VERSION     KHUT01001115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtgcgg
       61 taagtctgta gtgaaatctc tgggctcaac tcagagactg ctatagaaac tgccgtgctc
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001116
VERSION     KHUT01001116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           87 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cacccgaaac tacattgcta
      121 gagtatggga gagggaggcg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcctcctgga ccaatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001117
VERSION     KHUT01001117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggctgag
       61 caagtcgagt gtgaaatccc cgggcttaac ttgggaattg cgctcgaaac tacttggctg
      121 gagtatggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccttctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001118
VERSION     KHUT01001118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           79 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggatcgg
       61 taagtccgtg gtgaaatccc tgagcttaac tcaggaactg ccgtggatac tatcgatctt
      121 gaatgccgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat agcgaaggca gcttgctaca cggtaattga cactcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001119
VERSION     KHUT01001119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacgggggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgattcta
       61 taagtcttgt gtgaaagccc agagcttaac tctggaagtg catgagatac tgtaggattt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgttgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001120
VERSION     KHUT01001120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           95 g           39 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccagg
       61 caagtggaag gtgaaatccc tcggctcaac cgaggaactg ccctccaaac tgccgagctc
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001121
VERSION     KHUT01001121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c          103 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttcgt
       61 taagttgctg gtgaaatccc ggggctcaac tccggggctg ccagtaagac tggcgggcta
      121 gagcacggta gaggcggatg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaataccggt ggcgaaggcg ttccgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001122
VERSION     KHUT01001122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c          100 g           41 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggctggc
       61 taagccggat gtgaaagccc tgggctcaac ctgggaatgg catccgggac tggtcggctg
      121 gagtgcggta gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcgat ggcgaaggca gccacctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001123
VERSION     KHUT01001123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatccc acggctcaac cgtggaactg ccttagatac tgacggcctc
      121 gagggagata ggggcgagcg gaactgtgag tggagcggtg aaatgcgttg atattcacag
      181 gaactccggt ggcgaaggcg gctcgctgga tcttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001124
VERSION     KHUT01001124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtcagtg gtgaaatctc cgtgcttaac atggaaactg ccattgatac tataggtctt
      121 gaattttctg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca gggatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001125
VERSION     KHUT01001125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 caagtccgac gtgaaatctg gaggctcaac ctccagcggt cgttggaaac tgcctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaagca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001126
VERSION     KHUT01001126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggtcatg
       61 taagacagat gtgaaatccc cgggcttaac ctgggaattg catttgtgac tgcatggcta
      121 gagtgtggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca gtcccctggg cctgcactga cactcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001127
VERSION     KHUT01001127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtg
       61 caagtcgcgt gtgaaagccc ccggctcaac tggggagggt cacgcgatac tgctcgactg
      121 gaaggcagga gagggaagca gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001128
VERSION     KHUT01001128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcccgc
       61 taagtcacac gtgaaatccc tcagcttaac tggggaactg cgtgtgagac tggcaggctc
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgtatctga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001129
VERSION     KHUT01001129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggttcaa
       61 taagtcggtt gtgaaagtcc agggctcaac cctgggatgc cgatcgatac tgttggacta
      121 gagtccggaa gaggtgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcactggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001130             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001130
VERSION     KHUT01001130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           44 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctcat
       61 gcagtcggct ttgtgaaagc ccggggctca accccggagg tgcggccgat actcatgggc
      121 tcgagtccgg aagaggaggg tggaataccc agtgtagagg tgaaattcgt agatattggg
      181 tggaacaccg gtggcgaaag cggctctctg gtccggcact gacgctgagg cgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01001131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001131
VERSION     KHUT01001131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          101 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctggg
       61 taagtaaggg gtgaaatctc acggctcaac cgtggggctg cctcttcgac tgcccggctc
      121 gagcatgaca gaggtcggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaatatcggt ggcgaaggcg gccgactggg tcattgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001132
VERSION     KHUT01001132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgtcg
       61 caagtcggta gtgaaatctc caggctcaac ttggagtctg ctgccgaaac tgcggtgctt
      121 gagtactgga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga cagtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001133
VERSION     KHUT01001133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           88 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatca ttgggcgtaa agggtcctga ggtggtttgc
       61 taagtctact gtgaaagatc agggctcaac cctgaagatg cggtggaaac tggcaggcta
      121 gaatacggta ggggcagggg gaattcccgg tgtagcggtg aaatgcatag atatcgggaa
      181 gaacaccaaa ggcgaaggca ccctgctggg ccgatattga cactgacaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01001134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001134
VERSION     KHUT01001134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           98 g           49 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtggcc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgggttgcta
      121 gagtgtggga gaggctagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctggctgga ccactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001135
VERSION     KHUT01001135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           97 g           45 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcatac
       61 caagtattca gtgaaatctg tcggctcaac cgagagggtg ctgagtagac tggtaagcta
      121 gaggtcggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgatcctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001136
VERSION     KHUT01001136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           87 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttttt
       61 aaagtcttct gtcaaagatc agggcttaac cctgggtcgg cagaagaaac ttaaaagctg
      121 gagtctggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaat ggcgaaggca ctctgctggg ccacgactga cactgagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001137
VERSION     KHUT01001137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattag
       61 taagttgggg gtgaaatccc ggggctcaac ctcggaactg ccttcaaaac tactagtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001138
VERSION     KHUT01001138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggacact
       61 taagtcagag gtgaaatccc aaggctcaac cttggaactg cctttgatac tgggtgtctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001139
VERSION     KHUT01001139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          102 g           41 t
ORIGIN      
        1 tacggagggg gcgagtgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcatgg
       61 caagtcgagt gtgaaatccc ccggcttaac tggggaatgg cgctcgaaac taccgcgctg
      121 gagggcggga gagggaagtg gaactctcgg tgtaggggtg aaatctgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctggc ccgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001140
VERSION     KHUT01001140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggatagt
       61 taagtgagag gtgaaatccc agggctcaac cctggaactg cctttcatac tggctatctt
      121 gagtccggaa gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001141
VERSION     KHUT01001141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaatccc agggctcaac cctggaagtg cattcgaaac tgcttcgcta
      121 gagtactgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001142             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001142
VERSION     KHUT01001142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c          101 g           39 t
ORIGIN      
        1 tacggagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacta
       61 gcaagtcggg agtgaaatct cggagctcaa ctccgaggct gcttccgaaa ctgcggacct
      121 ggagactggt agaggtgggt ggaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacaccgg tggcgaaggc ggcccactgg gccagttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001143
VERSION     KHUT01001143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           79 g           62 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattaa
       61 taagtcagcg gtgaaaacct tcagcttaac tggagacttg ccattgatac tgttagtctt
      121 gagtacggtc aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactggg ccattactga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001144
VERSION     KHUT01001144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           92 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattga ttgggcgtaa agcgtctgta ggtggttacg
       61 ctagtcggct gttaaagatc agggctgaac cctgagcaag cagtcgaaac tgcgtggctg
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccaaa ggcgaaagcg ctctgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001145             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001145
VERSION     KHUT01001145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           97 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagttggat gtgaaatctc caggctcaac ttggaggggt cattcgatac tgccatgact
      121 agagttcggt aggggagtgt ggaattcctg gtggagcggt gaaatgcgca gatatcagga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001146             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001146
VERSION     KHUT01001146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           61 c           95 g           38 t
ORIGIN      
        1 gacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcccg tgtgaaatcc cccggctcaa ccggggaacg gccgggcata ctggcggtct
      121 tgggccatct aggggcagcc ggaacaaacg gtggagcggt gaaatgcgta gatatcgttt
      181 ggaacgccga cggtgaaaac aggctgctgg ggatgtcgcc gacgctgaga cacgaaagcc
      241 aggggagcaa acggg
//
LOCUS       KHUT01001147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001147
VERSION     KHUT01001147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcgggg gtgaaatcct gaggctcaac ctcagaactg cctccgatac tggcaatctc
      121 gagttcggaa gaggttggtg gaacagctag tgtagaggtg aaattcgtag atattagcta
      181 gaacaccagt ggcgaaggcg gccaactggt ccgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001148
VERSION     KHUT01001148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacgggaggt gcaagcgttg ttcggagtga ctgggcgtaa agcgcacgta ggtgggcttg
       61 taagtcgaat gtgaaatccc tgggcttaac ctgggaactg catccgaaac tacaagtctt
      121 gaatgcttca gaggttggta gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaataccgga ggcgaaggcg gccaactggg aatgcattga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001149
VERSION     KHUT01001149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtccgt
       61 taagtcgaat gtgaaatccc ccggctcaac cggggaagtg catccgaaac tggcgggcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001150
VERSION     KHUT01001150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           99 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtccgc
       61 taagtctgat gtgaaatccc ggggctcacc cccggaaggg cattggaaac tggcggactc
      121 gagtcccgga gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001151
VERSION     KHUT01001151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           87 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatg
       61 caagacagat gtgaaatccc cgggctcaac ctgggaactg catttgtgac tgcatgacta
      121 gagtacggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001152             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001152
VERSION     KHUT01001152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          105 g           33 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacca
       61 gcaagtcgga agtgaaatcc cggggctcaa ctccggggct gcttccgaga ctgcggacct
      121 ggggaccggt agaggccggt ggaattcccg gtgtagcggt ggaatgcgta gatatcggga
      181 agaacacccg tggcgaaggc ggccggctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001153
VERSION     KHUT01001153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggat gtgaaacctc ggggctcaac cccgagcctg catacgatac gggcagactt
      121 gagtacggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001154
VERSION     KHUT01001154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa aggggttgta ggcggccaac
       61 taagtccgac gtgaaatccc aaggcttaac cttggaactg cgtcggatac tggatggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacattga cgctgagaac cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001155
VERSION     KHUT01001155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           93 g           43 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgcgagca ggcggacctg
       61 taagtggatt gtgaaatctc ccggctcaac tgggagaggc cggtccagac tgcgggtcta
      121 gaggacggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccgtctctga cgctgagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001156
VERSION     KHUT01001156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggctag
       61 taagtccgtg gtgaaatccc tgagcttaac ttgggaactg ccattgatac tattagtctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca cggtcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001157
VERSION     KHUT01001157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttt
       61 taagtcagcg gtgaaagccc ggggctcaac cccggaactg ccgttgatac tgggaggctt
      121 gagtacagac gaggtaggcg gaattgaggg tgtagcggtg aaatgcttag ataccctcaa
      181 gaacaccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001158
VERSION     KHUT01001158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggcggcttgt
       61 taagttccgt gtgaaatccc caggcttaac ctgggaagtg cgcggaaaac tggcgagctt
      121 gaatgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggacattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001159
VERSION     KHUT01001159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ctgggcgtaa agagttcgta ggcggtttgt
       61 cacgtcgttt gtgaaaactc acagctcaac tgtgagcctg caggcgatac gggcagactt
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggaa cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001160
VERSION     KHUT01001160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccgt
       61 tacgtcggga gtgaaaactt ggggctcaac cccaagcttg ctttcgatac gggcggacta
      121 gaggtaggca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001161             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001161
VERSION     KHUT01001161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 taagacaggt gtgaaatccc cgggctcaac ctgggaatgg cgcttgtgac tggcgggctt
      121 agagtgcggc agaggggggt ggaattccac gtgtagcagt gaaatgcgta gagatgtgga
      181 ggaacaccga tggcgaaggc agccccctgg gccgacactg acgctcatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001162
VERSION     KHUT01001162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           49 t
ORIGIN      
        1 gacgtagggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggattcg
       61 taagtcgggg ttgaaatatc cgggctcaac tcggggcacg gcctcgatac tgcggatctc
      121 gagtcaggaa gagggtaatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttacctggt cctgcactga cgctgatgca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001163
VERSION     KHUT01001163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacaggc gtgaaatccc cgggcttaac ctgggaattg cgcttgtgac tgcaaggctg
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001164
VERSION     KHUT01001164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           93 g           54 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagttcggt gtgaaatctc ctggctcaac tgggagggtg cgccggaaac tggagtgctt
      121 gagcgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctttctgga ccatagctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001165
VERSION     KHUT01001165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttctt
       61 taagtccggg gtgaaagccc gttgctcaac aacggaactg ccctggaaac tggcgaactt
      121 gagtacagac gagggtggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001166             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001166
VERSION     KHUT01001166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           44 t
ORIGIN      
        1 cacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 caagtcgggt gtgaaaactc cgggctcaac ccggagacgc cacccgatac tgtcgtgact
      121 ggagtccggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001167
VERSION     KHUT01001167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggattca ctgggcataa agcgcacgta ggcggctcgt
       61 ttagtcagtt gtgaaatccc ccggctcaac cggggaatgg catctgatac tggcgggctc
      121 gaggtcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactagg ccgactctga cgctaaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001168
VERSION     KHUT01001168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcatg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccatggatac tatgtgtctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001169
VERSION     KHUT01001169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           99 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgg
       61 taagtcactt gtgaaatctc cgggcttaac tcggaacggt caagtgatac tgctgtgcta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001170
VERSION     KHUT01001170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggttcgg
       61 aaagtcggat gtgaaaactc ggagctcaac tccgggcctg catccgatac tctcggacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01001171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001171
VERSION     KHUT01001171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctctt
       61 caagtcgagt gtgaaatctc caggcttaac ctggaaaggt cgcttgatac tgttgagcta
      121 gagtgtggaa ggggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001172
VERSION     KHUT01001172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcttgt
       61 caggtctgat gtgaaatctt aaggctcaac cttaagcgtg catcggaaac cggcttgcta
      121 gagtctggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt tgcgaaggcg gctctctgga acagtactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01001173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001173
VERSION     KHUT01001173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcttgt
       61 caagtcactt gtgaaatctc tgggcttaac ccagaacggc caagtgaaac tgacatgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001174
VERSION     KHUT01001174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttta
       61 tatgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tgtagaacta
      121 gagtatatga gagggtgtcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gacacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001175
VERSION     KHUT01001175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggctgtgctg
       61 taagtcaggc gtgaaagccc cgggctcaac ctgggaattg cgcttgatac tgcggcgctg
      121 gaaggtggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggc ccacatttga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001176
VERSION     KHUT01001176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           91 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcgggtcct
       61 taagtcggtg gtgaaatcct ggagctcaac tccagaactg cctccgatac tgagggcctc
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001177
VERSION     KHUT01001177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggccctg
       61 taagtcttgg gtgaaatccc tcggctcaac cgaggaattg ccttggatac tgcagggctc
      121 gaggctggaa gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001178
VERSION     KHUT01001178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 caagtcgagt gtgaaatccc ccggctcaac tggggagggt cgctcgatac tggatgactc
      121 gaaggcagga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001179
VERSION     KHUT01001179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtaacc
       61 taagcgtgcg gtgaaagccc gaggctcaac ctcgggtcgg ccgtgcgaac tgggtcactc
      121 gagcagaata gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ttctggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001180
VERSION     KHUT01001180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           89 g           46 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcgatg
       61 caagtcaggt gtgaaagccc acggctcaac cgtggaacag cgcttgaaac tgcattgctg
      121 gagtgagcta gaggtatgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaagcg gcatactgga gcttgtctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001181
VERSION     KHUT01001181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgagg
       61 caagtctggt gtgaaatccc ggggctcaac cccggaactg cactggatac tgctttgcta
      121 gagtactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagc ggcgaaggcg agttcctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001182
VERSION     KHUT01001182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          101 g           42 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattca ctgggcgtac agggtgtgta ggcggtttgg
       61 taagccttct gttaaagctt cgggcccaac ccggaaagcg cagagggtac tgccaggcta
      121 gagggcggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtacctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001183
VERSION     KHUT01001183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           88 g           44 t
ORIGIN      
        1 tacagagact gcgagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccaag
       61 tgtgtgaggc gtgaaagccc ggggcttaac cccggaattg cacctcaaac tacatggcta
      121 gagcattgga gagggtagca gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaataccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01001184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001184
VERSION     KHUT01001184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccatt
       61 caagtcgggg gtgaaagccc agggctcaac cctggaattg ccttcgatac tgtttggcta
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001185
VERSION     KHUT01001185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           92 g           40 t
ORIGIN      
        1 gacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgcc
       61 taagtgtctt gtgaaatccc acggctcacc cgtggaactg ctgggcatac tgggcgtctt
      121 gggccaacta ggggcagccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggctgctggg gttgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001186
VERSION     KHUT01001186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaactg cgctctaaac tgtcgtgctt
      121 gaggaagcta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg gcttatctga cgctgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001187
VERSION     KHUT01001187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatca ctgggcgtaa agcgtgcgta ggcggtttct
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaacgg cgatggatac tgggaagcta
      121 gaatgtgaga gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccatctggc tcaacattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001188             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001188
VERSION     KHUT01001188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           49 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcagt
       61 gcaagtcagt tgtgaaatct ccgggcttaa ctcggaaagg tcaactgata ctgctcgact
      121 agagtgcgga aggggcaact ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaagt gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001189
VERSION     KHUT01001189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtttg
       61 taagtcagtg gtgaaatctc caggcttaac ctggaaactg ccattgatac tatagatctt
      121 gaattttctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca ggaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001190
VERSION     KHUT01001190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggcggccgca
       61 taagttggac gtgaaaactc cgggcttaac ttggagaggc cgttcaatac tgtgaggctg
      121 gagggtgtca gagggaagta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaatacccgt ggcgaaggcg gcttcctggg gcaccactga cgctgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001191
VERSION     KHUT01001191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtctgac gtgaaatccc cgggctcaac ctgggaattg cgttggagac tgcaaggcta
      121 gagtttggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaagactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001192
VERSION     KHUT01001192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           76 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaattg ccattgatac tgataatctt
      121 gagtacattt gaggcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001193
VERSION     KHUT01001193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggtggccctg
       61 taagtcaaag gtgaaatccc tcggctcaac cgaggaattg cccttgaaac tgcagggctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cactgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001194
VERSION     KHUT01001194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaaata ctgggcgtaa agcgaacgta ggcggcttag
       61 caagtcaggg gtgaaatccc agagctcaac tctggaactg cctttgaaac tgcttcgctt
      121 gaatcctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caggtattga cgctgaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001195
VERSION     KHUT01001195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgtagatg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggttctt
       61 taagtctgat gtgaaagccc ctggcttaac cagggagggt cattggaaac tggagaactt
      121 gagtgcagaa gaggaaagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001196
VERSION     KHUT01001196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccatt
       61 caagtcaggg gtgaaagccc aaggctcaac cttggaattg cctttgatac tgtttggctt
      121 gagaccggaa gaggtcggcg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccgactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001197
VERSION     KHUT01001197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcttta
       61 caagtcaggg gtgaaagccc gcggctcaac cgcggaattg cccttgagac tgtatcgctt
      121 gaacacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgtagttga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001198
VERSION     KHUT01001198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           95 g           40 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccagg
       61 taagtcggat gtgaaagccc ggggcccaac cccggaactg cacccgatac tgcctggctg
      121 gagtcccgga gaggatggcg gaatttccgg tgtagaggtg aaattcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001199
VERSION     KHUT01001199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgt
       61 cgcgtcggct gtgaaaacct gaggctcaac ctcaggcctg cagtcgatac gggcggactt
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001200             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001200
VERSION     KHUT01001200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcgggt gtgaaacctc caggctcaac ctggagacgc cactcgatac tgctatggct
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001201
VERSION     KHUT01001201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           92 g           41 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgca ggcggcttgg
       61 taagtcggat gtgaaagccc ggggctcaac cccggaactg cacccgatac tgcctcgcta
      121 gagtcccgga gaggatggcg gaatttccgg tgtagaggtg aaattcgtag atatcggaag
      181 gaacaccagc ggcgaaggcg gccatctgga cggtgactga cgctcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001202
VERSION     KHUT01001202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccctc
       61 taagtcagac gtgaaatccc acggcttaac cgtggaactg cgtctgatac tggagggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001203
VERSION     KHUT01001203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           85 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctgat
       61 taagtcagcg gtgaaagaca tcggctcaac cgatgcagtg ccgttgatac tggtcagctt
      121 gagtgttgca ggggtacatg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgaaggca ttgtactggg caacaactga cgctgatgca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001204
VERSION     KHUT01001204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          102 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttga
       61 caagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgccgagctt
      121 gagcacagta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg ctgtgtctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001205
VERSION     KHUT01001205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtagac
       61 taagtcggat gtgaaatccc agggctcaac cctggaactg cattcgatac tggtctgctc
      121 gaggactgga gaggagattg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001206
VERSION     KHUT01001206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ttgggcgtaa agggtgcgta ggtggcaaag
       61 taagttggat gtgaaatcct ggggcttaac ctcagaattg cattcaatac tgcttagcta
      121 gagagctgta gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg agtctctggg cagcatctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001207
VERSION     KHUT01001207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcccaaaac tgctgcacta
      121 gaggaagaca gaggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcgtactggg tcttatctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001208
VERSION     KHUT01001208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggatctt
       61 taagtcgggg gtgaaagccc agggctcaac cctggaattg ccttcgatac tgaagatctc
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001209
VERSION     KHUT01001209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           46 c          101 g           54 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggttgat
       61 tgcgtccgcc gtgaaaggcc tcggcttaac cggggttttg cggtggatac gggtcgactt
      121 gaggcaatta ggggagatcg gaattctacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gatctctggg attgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001210
VERSION     KHUT01001210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           86 g           52 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggactgg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgcttgtctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001211
VERSION     KHUT01001211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           73 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtggataag
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatttgtctt
      121 gaatactttg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca cagtcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001212             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001212
VERSION     KHUT01001212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           48 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcggat gtgaaatctc cacgcttaac gtggaggggc cattcgaaac tgctgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001213
VERSION     KHUT01001213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcaatt
       61 taagtcagtg gtgaaatcct gcagctcaac tgtagaacag ccattgaaac tgaattactt
      121 gagtaatctt gaggtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactaag gatttactga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001214             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001214
VERSION     KHUT01001214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          102 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctctt
       61 taagtcttac cgtgaaatgc cggggctcaa ccccgcgcac gtcgggggat actggggagc
      121 tggagacaag tagaggcgag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacacca gaggcgaagg cggctcgctg ggcttggtct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01001215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001215
VERSION     KHUT01001215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 caagtcagga gtgaaaggcc tgggctcaac cgaggaactg cttttgagac tgccgagcta
      121 gagtgcagaa ggggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001216             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001216
VERSION     KHUT01001216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          101 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtctacc cgtgaaatgc cggagctcaa ctccgtgcac gccgggggat actggaggac
      121 tggagacgag tagaggcaag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacaccg gaggcgaagg cggcttgctg ggcttggtct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01001217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001217
VERSION     KHUT01001217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggttcgg
       61 taagtcggat gtgaaaacac gaggctcaac ctcgtgcgtg catccgatac tgtcggacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001218
VERSION     KHUT01001218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctatc
       61 cacgtcggat gtgaaagctc agggctcaac cctgagaggc cattcgatac gggatagcta
      121 gaggtagcta ggggaaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg gcttacctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01001219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001219
VERSION     KHUT01001219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           60 c          111 g           37 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggcttgg
       61 cgcgcccgac gtgaaagccc ccggcttaac cggggagggt cgtcgggtac ggccaggctt
      121 gagggcggga gagggtggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccacctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001220
VERSION     KHUT01001220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 tcagtccctt gtgaaatccc ccggctcaac tggggaactg caagggaaac agcagggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001221
VERSION     KHUT01001221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 tacggggggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagttggat gtgaaaactc tgggcttaac ccagagcctg cattcaatac tgacaggcta
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagagtctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001222
VERSION     KHUT01001222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           84 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta gacggctttg
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgaaac tgcactgcta
      121 gagtatggaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg acttcctggt ccaatactga cgttcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001223             251 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001223
VERSION     KHUT01001223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>251
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           91 g           42 t
ORIGIN      
        1 gacggggggg gcaagtgttc ttcggaatga ctgggcgtaa agggcacgta ggcggtgaat
       61 cgggttgaaa gtgaaagtcg ccaaaaacag gcggaatgct ctcgaaacca attcacttga
      121 gtgagacaga ggagagtgga atttcgtgtg taggggtgaa atcagcagat ctacgaagga
      181 acgccaaaag cgaaggcagc tctctgggtc cctaccgacg ctggggtgcg aaagctaggg
      241 gagcgaacag g
//
LOCUS       KHUT01001224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001224
VERSION     KHUT01001224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           99 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgtc gtgaaagctt ggggcttaac cccaagtctg cggtggatac gggcagacta
      121 gaggcaggta ggggagaacg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001225
VERSION     KHUT01001225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           88 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgca ggtgggtatt
       61 taagtctggt ttgaaagcag gtggctcaac catctgatgt ggctggaaac tggatatctt
      121 gaatgggttg gcggtagccg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001226
VERSION     KHUT01001226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           93 g           48 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggtggtaatg
       61 taagtctggc gtgaaatttt tcggcttaac cgggaaaggt cgtcggagac tgcataacta
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cactgaggcg cgatagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001227
VERSION     KHUT01001227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcagct gtgaaagccc ggggctcaac cccggagagt cgactgatac tggcagacta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001228
VERSION     KHUT01001228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          104 g           40 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggacgg
       61 caagtcggtc gtgaaagccc tcggctcaac cgaggagagt cggccgagac tgtcgttctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg catttcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001229             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001229
VERSION     KHUT01001229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcggtc gtgaaagccc gaggctcaac ttcgggactg cggtcgatac tgctatagct
      121 agagtttggt agaggagaac ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacacctg tagcgaaggc ggttctctgg gccaatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001230             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001230
VERSION     KHUT01001230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          101 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccttt
       61 taagtctgac ggtgaaatgc cggggctcaa ccccgtgccc gccggagata ctgggaggct
      121 ggagacatgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcatgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001231
VERSION     KHUT01001231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          108 g           35 t
ORIGIN      
        1 gacgtaggat ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggacacg
       61 cgcatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgagat ggcgtgtctg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctggctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001232             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001232
VERSION     KHUT01001232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           50 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcggat gtgaaaactc caggctcaac ttggagacgc cattcgatac tgcgatgact
      121 tgagtccggt aggggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg cggcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001233
VERSION     KHUT01001233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggctcgca ggcggtcttc
       61 taagtctgat gtgaaatctt gcggcttaac cgcaagcggt cattggaaac tggaggactt
      121 gagtacagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001234
VERSION     KHUT01001234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttgat
       61 taagtcccgt gtgaaatccc tcggctcaac tgaggaactg cacgggaaac tggccaactt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001235
VERSION     KHUT01001235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgca
       61 caggtccgtt gtgaaaactg gaggcttaac cttcagacgt cgatggaaac cgtgcggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001236
VERSION     KHUT01001236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggccgcacgg
       61 taagtcaggt gtgaaagccc ggagctcaac ttcggaattg cacccgatac tgccgtgctt
      121 gaggactgga gaggagtctg gaattcacag tggagcagtg aaatgcgtag atattgtgag
      181 gaacactagt ggcgaaggcg agactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001237
VERSION     KHUT01001237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcatgg
       61 taagttgaag gtgaaatctc ggagctcaac tccgaaactg ccttcaatac tgctgtgctt
      121 gaggactgta gaggagaatg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg attctctggg cagttcctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001238
VERSION     KHUT01001238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          102 g           45 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcctgt
       61 cgcgtccgtc gtgaaagccc ggggcttaac tccgggtctg cggtggatac gggcaggcta
      121 gaggcaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001239
VERSION     KHUT01001239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          101 g           43 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 cgagtgaggc gtgaaagtcc tgggctcaac ccggggacgg cgcttcagac tgcctggctg
      121 gagtccggga gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggc ccggtactga cgctcaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001240
VERSION     KHUT01001240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttgtt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaattg cgatggaaac tgggcaacta
      121 gagtgtggca gaggatagtg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacatccgt ggcgaaggcg actgtctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001241
VERSION     KHUT01001241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c           99 g           52 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcttcc gtgaaaactg ggggcttaac ttccagcttg cggtggatac gggctgactg
      121 gagtgcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001242             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001242
VERSION     KHUT01001242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           89 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 ttagtgtgaa gtgaaatctc cagggctcaa cccggaaact gctttgcaaa ctggcagact
      121 agaggaaggc agaggtgaat ggaattcctg gtgtagcggt gacatgcatt gatatcagga
      181 ggaacacccg tggcgaaggc ggttcactgg gccttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001243
VERSION     KHUT01001243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           84 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcaggg gtgaaagact attgcttaac aatggcagtg cctttgatac tgataggctt
      121 gagtacagct gaggcagatg gaacgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gtctgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001244
VERSION     KHUT01001244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           98 g           40 t
ORIGIN      
        1 gacggaaggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggcctgg
       61 taagtctggc gtgaaagccc agggctcagc cctggaagtg cgctagaaac tgccaagctg
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001245
VERSION     KHUT01001245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 caagtcagag gtgaaagacg gtagcttaac tatcgcagtg cctttgaaac tggtgggctt
      121 gaatgtactt gaggtgggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaaccccgat tgcgaaggca gctcactaaa gtatgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001246             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001246
VERSION     KHUT01001246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggta gttgaaagcc cccggctcaa ccggggaagt ggtgccgaaa cgacccgtct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctactg acgctgagac gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001247
VERSION     KHUT01001247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agagcacgta ggcggcgcgc
       61 taagtcaggt gtgaaatccc ccggctcaac cggggaactg cacctgatac tggcgtgctc
      121 gaggacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtctctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001248
VERSION     KHUT01001248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           82 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcaatt
       61 taagtctgct gttaaagcat ctggcttaac cagataaaag cagtggaaac tagagagcta
      121 gagtgcggta ggggcagagg gaattaagaa agtagcggtg aaatgcgtag atactcttaa
      181 gaacaccagt ggcgaaagcg ctctgctggg cctgcactga cactaatgga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01001249             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001249
VERSION     KHUT01001249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccagc
       61 acaagtcagt tgtgaaatct ccgagcttaa ctcggaacgg tcaactgata ctgtgcggct
      121 agagtgcaga aggggcaact ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgatgc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001250
VERSION     KHUT01001250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           84 g           52 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttat
       61 taagtcttct gtcaaagctc agggcttaac cctgtttagg cagtagaaac taataggcta
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacaccgat ggcgaaagca ctctgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001251
VERSION     KHUT01001251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           89 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctag
       61 caagtaggga gtgaaagccc tcggcttaac cgaggaagtg ctttctaaac tactaggctt
      121 gaatactgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001252
VERSION     KHUT01001252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          102 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccttc
       61 tcagtcgggt gtgaaagccc ggggctcaac cccggaacgg cacccgagac gggttggctg
      121 gagaccggga gaggagggtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001253
VERSION     KHUT01001253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agggcgtgta ggcgggaagg
       61 caagtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac tgcctttctt
      121 gagtgtcgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001254
VERSION     KHUT01001254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           85 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcgcag
       61 taagtcagga gtgaaagttt gcggctcaac cgtaaaattg cttttgatac tgctgtgcta
      121 gaattaggat gaggttagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccaat tgcgaaggca gctggctaga cctatattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001255
VERSION     KHUT01001255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agagctcgta ggtggctctg
       61 taagtaggtg gtgaaagccc acggctcaac cgtggatacg cctcctaaac tgtggagctt
      121 gaggctggga gaggaaatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gatttctgga ccagttctga cactgaggag cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01001256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001256
VERSION     KHUT01001256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgagg
       61 taagttcgat gtgaaatctc cgagctcaac tcggaaatgg cattgaatac tatcccgctt
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001257
VERSION     KHUT01001257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           82 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggccct
       61 taagtcagtg gtgaaagctc cgagcttaac tcggaaactg ccattgatac tataggtctt
      121 gaattcggtc gaggttggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctagctaga ccgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001258
VERSION     KHUT01001258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgatt gtgaaacccc agggctcaac cctgggcctg cgttcgatac gggcaagcta
      121 gagtatggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001259
VERSION     KHUT01001259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgct gtgaaatcct ggggctcaac cccgggcgtg cagcggatac tgccgggcta
      121 gagggtggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccactcctga cgctgagacg cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01001260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001260
VERSION     KHUT01001260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggtgggg
       61 taagtcggat gtgaaatctc ggagctcaac tccgaaatgg cattggaaac tgctctgctg
      121 gagggtcgga ggggggactg gaatgcttgg tgtagcagtg aaatgcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg agtccctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001261
VERSION     KHUT01001261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           97 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccgaa
       61 caagtcactt gtgaaatctc cgggcttaac tcggaaaggt caagtgatac tgtttggcta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001262
VERSION     KHUT01001262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattag
       61 aaagtccgtt gtgaaatctc tgagcttaac tcggaaattg cggtggatac tattaatctt
      121 gaatactgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cagatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001263
VERSION     KHUT01001263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agggcgtgca ggcggtcttt
       61 taagttaggc gtgaaagctc ccggctaaac cgggagaggt cgtctgatac tgggagacta
      121 gagtatggta gaggcgtgta gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaataccagt ggcgaaggcg gcacgctggg ccataactga cgctaagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001264
VERSION     KHUT01001264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           86 g           49 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtatgt
       61 taagttcgtt gttaaaggct ccggcccaac cggagtattg cgatgaaaac tggcacacta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001265             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001265
VERSION     KHUT01001265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           48 t
ORIGIN      
        1 cacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttggg
       61 taagtcgggt gtgaaaactc caggctcaac ttggagacgc cacccgatac tgctctgact
      121 tgagttcggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001266
VERSION     KHUT01001266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           95 g           52 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgag
       61 caagttagat gtgaaagtcc tgggcttaac ctggggactg catttgatac tgcttggctt
      121 gaatgcagga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctatctgga ctgtgattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001267
VERSION     KHUT01001267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           45 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggctgaa
       61 taagtgggat gttaaatccc ccggctcaac cggggaactg catctcatac tgttcagctt
      121 gggtacagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg ctgataccga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001268
VERSION     KHUT01001268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcgggttat
       61 caagttggag gtgaaagccc agggctcaac cttggaattg ccttcaaaac tgatagccta
      121 gagtatgata gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggg tcatgactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001269
VERSION     KHUT01001269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           87 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaaata ctgggcgtaa agcgcacgta ggcggtggag
       61 taagtcagag gtgaaatccc agggctcaac cctggaactg cctttgaaac tgctacacta
      121 gaatcatgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001270
VERSION     KHUT01001270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcccgt
       61 taagtccggg gtgaaagccc acagctcaac tgtggaactg ccctggatac tggcgggctt
      121 gagagtcgtc gagggtggcg gaatggacgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccaat tgcgtaggca gctgcctagg cgacatctga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001271
VERSION     KHUT01001271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           91 g           41 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctcca
       61 aaagtcggat gtgaaatccc tcggctcaac cgaggacgtg catccgaaac tgcggagctt
      121 gagtatggaa gagggtcgcg gaatccccgg tgtagaggtg aaattcgtag atatcggggg
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001272
VERSION     KHUT01001272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggcgatctt
      121 gggccattca ggggcgactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggtcgctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001273
VERSION     KHUT01001273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggcttcc
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaattg catccgaaac tgggtggcta
      121 gagtacggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc ccagtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001274
VERSION     KHUT01001274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggctttg
       61 taagttggct gtgaaatctc ccggctcaac tgggagggga cggtcaagac tgcaaggctg
      121 gaggcaggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga cctgtcctga cgctgagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001275
VERSION     KHUT01001275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           90 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcgtct
       61 taagtctggt ttgaaagctg gtcgctcaac gatcagatgt ggctggaaac tggggcgctt
      121 gaatgggttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001276
VERSION     KHUT01001276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttcag
       61 taagatgggt gtgaaatccc cgggctcaac ctgggaactg ctttcataac tgctgaacta
      121 gagtacggta gagggtagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001277
VERSION     KHUT01001277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          100 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctcgt
       61 taagtcgggg gtgaaaaccc ggagctcaac tccgggcctg cctccgatac tggcgggctg
      121 gaggcaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001278
VERSION     KHUT01001278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatagt
       61 taagtcaggg gtgaaatccc ggggcccaac ctcggaactg cctttgatac tggctatctg
      121 gaggatggaa gaggcaagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg acttgctggt ccatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001279
VERSION     KHUT01001279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          108 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggaagcg
       61 ggcgtcggtg cggaaaggtc gccgcttaac ggcggagagc gtaccgatac gccgcttctg
      121 gagcgcgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt gggggaaccg gccacctagc ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01001280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001280
VERSION     KHUT01001280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagcgtgta ggcggttagg
       61 taggtcagtt gtgaaaactg gaggctcaac cttcagacgt cgactgaaac cccctggcta
      121 gagtccggga gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaagcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001281
VERSION     KHUT01001281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcatgca ggcggatcga
       61 taagtcgggg gtgaaagccc gaggctcaac ctcggaactg ccttcgatac tgtcgatctt
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001282
VERSION     KHUT01001282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           82 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggtctt
       61 taagtcagtg gtgaaagccg acagctcaac tgtcgaaatg ccattgatac tggggatctt
      121 gagtacagta gaggtaggcg gaattaatgg tgtagcggtg aaatgcatag ataccattaa
      181 gaacaccgat tgcgaaggca gcttactata gtgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001283
VERSION     KHUT01001283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggttatc
       61 taagtcggat gtgaaagccc tcggcttaac tgaggaagtg cacccgaaac tggatagcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001284
VERSION     KHUT01001284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctttt
       61 taagtcaggg gtgaaatccc ggtgctcaac accggaactg cccttgagac tggatcgctt
      121 gatcacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgttgatga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001285
VERSION     KHUT01001285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 tgcgtcggct gtgaaaaccc ggggctcaac cccgggcctg cagccgatac gggcagacta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001286
VERSION     KHUT01001286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           97 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgt
       61 cgcgtcggct gtgaaaaccc ggagctcaac tccgggcctg cagtcgatac gggcggactt
      121 gagtgttgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caactactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001287
VERSION     KHUT01001287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacggaatgt gcaaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggcttgt
       61 taggtgaggt gtgaaatccc tcggcccaac cgaggaattg cgcttcaaac cggcaagcta
      121 gaggaggata gaggtgggcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gtccactggg tcctttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001288
VERSION     KHUT01001288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattgg
       61 taagtccgtg gtgaaatccc cgagcttaac ttgggaactg ccgtggatac tatcagtctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgtaggca gcttactact cgatcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001289
VERSION     KHUT01001289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcctga
       61 taagtaggag gtgaaatcct ggggcttaac ctcagggctg ccttctagac tgtccggctc
      121 gagcacagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ctgttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001290
VERSION     KHUT01001290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcaggg gtgaaagact gcagctcaac tgtagcagtg cctttgatac tgatgagctt
      121 gagtacagat gaggctggcg gaatgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gccggctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001291
VERSION     KHUT01001291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           85 g           55 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattca ctgggtgtaa agggagcgca ggcgggtttg
       61 taagtcggtg gtgaaatcct caggcttaac ctgagaactg ccaccgatac tgtaaatctt
      121 gagtgtggaa gaggccgatg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccgat ggcgaaggca gtcggctggt ccattactga cgctcatgct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001292
VERSION     KHUT01001292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           94 g           41 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcccg
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgcgggtctt
      121 gggccaacta ggggcagccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggctgctggg gttgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001293
VERSION     KHUT01001293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggacatt
       61 taagtcaggg gtgaaagcct gaggctcaac ctcagaattg cctttgatac tgggtgtctc
      121 gagaccggta gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001294
VERSION     KHUT01001294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttatt
       61 caagtctggt gttaaatact ggcgctcaac gccagcatgg cattggaaac tggatagcta
      121 gagtgtggta gaggttacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtaactggg ccataactga cgctgaggaa cgaaagccag
      241 ggtagccaaa ggg
//
LOCUS       KHUT01001295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001295
VERSION     KHUT01001295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          106 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 tgagtcggtg gtgaaagctc ggggctcaac cccgagtcgg ccatcgatac tgccgggctc
      121 gagcatcgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cgatggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001296             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001296
VERSION     KHUT01001296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          101 g           44 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggctgct
       61 caagtcaggc gtgaaaggcc tgggctcaac ccgggaattg cgcttgagac tgggctggct
      121 tgagtgcggg agaggatggt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggccatctgg accgcaactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001297             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001297
VERSION     KHUT01001297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcttgg
       61 aaagtcgggt gtttaagctc ggggctcaac cccgatacgc acccgaaact gccaggcttg
      121 agtgcagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001298
VERSION     KHUT01001298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgta
       61 taagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgttcgactt
      121 gaaggtagga gagagaagca gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcttcttggc ctattcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001299
VERSION     KHUT01001299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtgcaa
       61 taagtcaggc gtgaaagccc cgggctcaac ccgggaattg cgcttgatac tgttgcgctt
      121 gaatccagga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccatctggc ctggaattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001300
VERSION     KHUT01001300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcaag
       61 taagtgggat gtgaaatgcc ccggctcacc cggggacctg catctcaaac tgcttggctc
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001301
VERSION     KHUT01001301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaggg
       61 catgtcggat gtgaaatccc gtggctcaac cacggaagtg cacccgaaac tgctcagctg
      121 gagtctcgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001302
VERSION     KHUT01001302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggtcatc
       61 tacgtctggg gtgaaagccc ggagctcaac tccgggattg ccttggatac gggatgactt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001303
VERSION     KHUT01001303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agagctcgta ggccgctctg
       61 taagtcggaa gtgaaagacc acggctcaac cgtggaaacg ctttcgaaac tgtggagctt
      121 gaggctggga gaggagatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gatctctgga ccggttctga cgctgaggag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001304
VERSION     KHUT01001304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          102 g           42 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggaggg
       61 cgagtcggtc gtgaaatcct tcggctcaac cggagaacgt cggccgatac tgcctttctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg catctcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001305
VERSION     KHUT01001305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          108 g           37 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtgcga
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcggac tgtcggactg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001306
VERSION     KHUT01001306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           87 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctatc
       61 taagtcggat gtgaaatccc tcggcttaac caaggaagtg cacccgaaac tggatagctt
      121 gagtacagaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001307
VERSION     KHUT01001307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           91 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgctc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggagtgctt
      121 gagtatggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttgctgga ccatcactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001308
VERSION     KHUT01001308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           85 g           56 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agcgaacgta ggcggttact
       61 taagtttggt cttaaagacc ggggctcaac tccgggaatg gactgaatac tgggtgacta
      121 gaggatgtca gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg gctttctggg acattcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01001309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001309
VERSION     KHUT01001309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           53 c          109 g           42 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcctga
       61 taagggtgtg gtgaaagtcc ggggctcaac cccggatcgg ccgtgccgac tgttgggctg
      121 gagtactgta ggggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggag
      181 gaagaccggt ggcgaaggcg gcctgctggg cagttactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001310             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001310
VERSION     KHUT01001310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcacg
       61 acaagtcagt tgtgaaatct ctgggcttaa ctcagaacgg tcaactgata ctgtcggact
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001311
VERSION     KHUT01001311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtttgat gtgaaatctc tgggctcaac ccagaaatgg cattgaatac tatcttgcta
      121 gaggagtgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cacttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001312
VERSION     KHUT01001312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctat
       61 taagtcagac gtgaaatccc tcggctcaac cggggaactg cgtctgatac tggtgggctt
      121 gagttcggga gaggaatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001313
VERSION     KHUT01001313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          103 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcagg
       61 taagggtgtg gtgaaagtcc ggggctcaac cccggatctg ccgtgccgac tgcttgactg
      121 gagcattgca gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaagaccggt ggcgaaggcg atctgctggg caatggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001314
VERSION     KHUT01001314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttact
       61 taagtagggt gtgaaatgtc ccggctcaac cggggaatgg cgctctaaac tgggtggctt
      121 gagtgagata gaggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggagg
      181 gaacaccatt ggcgaaagcg gcacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001315
VERSION     KHUT01001315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          104 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccttt
       61 taagtctcac cgttcaagcc cggggctcaa ccccggtccg cgggggatac tgggaggcta
      121 gggacatgta ggggcgagcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggg ggcgaaggcg gctcgctggg catggtccga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001316
VERSION     KHUT01001316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctaa
       61 taagtcagtg gtgaaagcct ccagcttaac tggagaactg ccattgatac tgttgggctt
      121 gagtatactt gaggtaggcg gaattgacag tgtagcggtg aaatgcatag atattgtcaa
      181 gaacaccaat tgcgaaggca gcttactaaa ttataactga cgctgaggca cgaaagtgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01001317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001317
VERSION     KHUT01001317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           79 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggctata
       61 taagtcagtg gtgaaagccc gcagctcaac tgcggaattg ccattgaaac tgcatagctt
      121 gaatctggtt gaagtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaag ccaacattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001318
VERSION     KHUT01001318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           98 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggct gtgaaaactc ggggctcaac tccgagcttg cagtcgatac gggcaggcta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001319
VERSION     KHUT01001319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 cacgggggga gcgagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 taagtgggat gtgaaagccc tcggctcacc cgaggaaagt catctcatac tgctggactt
      121 gagtgcggga gaggatggag gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg tccatctggc ccgttactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001320
VERSION     KHUT01001320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           83 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgg
       61 aaagaaagat gtgaaatccc agggctcaac cttggaactg catttttaac taccggacta
      121 gagtatgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ataatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001321
VERSION     KHUT01001321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c          103 g           39 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggatggg
       61 taagtgggtc gtgaaagcgc cgggctaaac cgggcgagga cgaactagac tgcctgtcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001322
VERSION     KHUT01001322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           90 g           51 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggtggttgtc
       61 gtagtcaggc gtgaaagcct tgagctcaac tcaagaaatg cgcttgatac tgggcagcta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cactaagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001323
VERSION     KHUT01001323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           93 g           54 t
ORIGIN      
        1 cacgggggga acgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcgggttgg
       61 caagtcgggt gtgaaattcc atagcttaac tatggacctg cattcgaaac tgttgatctt
      121 gagtactgga gaggtgagtg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccgtt ggcgaaggcg gctcactgga cagatactga cgctgaggca cgaaggtcgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001324
VERSION     KHUT01001324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           82 g           58 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgtgtgta ggtggtttgc
       61 taagttgggt gttaaatctc tcggctcaac cgagagttgt cgctcaaaac tagtagactt
      121 gaggacggta gaggaaggta gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccagt ggcgaaggcg gccttctgga ccgttcctga cactcagaca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001325
VERSION     KHUT01001325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggtccgc
       61 taagtcgcgt gtgaaatccc ccggctcaac cggggaaatg cgcgtgatac tggcgggctt
      121 gagatcggga ggggtgaatg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gttcactggc ccgacactga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001326
VERSION     KHUT01001326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          100 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggccgct
       61 tgcgtcggat gtgaaagccc ggggctcaac cccggaactg catccgatac ggggtggctc
      121 gagaccgaga gaggtgagtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctcactggc tcggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001327
VERSION     KHUT01001327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c           99 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggttcgt
       61 taagtcgggt gtgaaaaccc ggggctcaac cccgggcctg catccgatac tggcggactc
      121 gaggcagaca ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg tctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001328
VERSION     KHUT01001328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           85 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgagcgta gtcggttatt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaattg cattcgatac tggatagcta
      121 gagttcggca gagggaagcg gaatttcggg tgtagcggtg aaatgcgtag atatccgaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccagaactga cgatcaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001329
VERSION     KHUT01001329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggctct
       61 taagtcaggg gtgaaagccc aaggctcaac cttggaattg cctttgatac tgagggcctt
      121 gagaccggaa gaggtcggcg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccgactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001330
VERSION     KHUT01001330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccaac
       61 taagtcagac gtgaaatccc tcggcttaac cggggaatgg cgtctgatac tggatggctt
      121 gagttcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgagactga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001331
VERSION     KHUT01001331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           80 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgca ggcggatttg
       61 taagtcagtg gtgaaaggct taagcttaac ttgggaactg ccattgatac tataagtctt
      121 gaatgttgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaaa caaatattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001332
VERSION     KHUT01001332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           87 g           51 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggattca ttgggcttaa agggtacgta ggcggcgcta
       61 tcagtcgggt gtgaaagccc tcggctcaac cgaggaactg cgcccgatac tgtagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001333
VERSION     KHUT01001333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           84 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgtgg
       61 aaagtcgaat gtgaaatctc ggggcttaac cccgaaactg cattcgatac taccatacta
      121 gaggattgta gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaagcg gatctctggg caactcctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001334
VERSION     KHUT01001334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggccctg
       61 taagtccgtg gtgaaagcct ctcgcttaac gagagaactg ccatggatac tgcagggctt
      121 gagtccagac gaggttggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001335
VERSION     KHUT01001335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgcgta ggcggtccgg
       61 taagtcggtt gtgaaagtcg ggggctcaac cctcggatgc cgatcgatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001336
VERSION     KHUT01001336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtcagg
       61 caagtcggat gtgaaatccc gtggctcaac tacggaagtg cacccgaaac tgcttgactt
      121 gagtcccgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgaggactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001337
VERSION     KHUT01001337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           78 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaaggg
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaactg ccattgatac tgctcatctt
      121 gagtacattt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001338             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001338
VERSION     KHUT01001338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           90 g           48 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcaatt
       61 caagtctgct gttaaagcgc agggcttaac tctgtaaagg cagtggaaac tgaatagcta
      121 gagtgtggta ggggcagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacatcggt ggcgaaggcg ctctgctgga ccataactga cactcacagg acgaaagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01001339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001339
VERSION     KHUT01001339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctgta
       61 caagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgtacggctt
      121 gaggccggga gggggtagcg gaattcccaa tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001340
VERSION     KHUT01001340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           91 g           51 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccgc
       61 caagtcaggg gtgaaatcct gcagcttaac tgtagaagtg cctctgatac tggcggtctt
      121 gagggaaata ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg gcgcactgga tttcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001341
VERSION     KHUT01001341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           78 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 gaagtcagtg gtgaaatcct agagcttaac tctagaactg ccgttgatac tctctgtctt
      121 gaatcccgtg gagatgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgttaca cgaagattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001342
VERSION     KHUT01001342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          100 g           42 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggtcctg
       61 tttgtcgagt gtgaaagccc cgggctcaac ctgggaactg cgctcgaaac ggcgggacta
      121 gagatcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001343
VERSION     KHUT01001343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           84 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta gacggttttg
       61 taagtcagat gtgaaatccc tgggctcaac ctgggaactg catttgatac tgcatggcta
      121 gagtatcgaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg acttcctggt cgaatactga cgttcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001344
VERSION     KHUT01001344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctatc
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaactg ccattgaaac tgtttagctt
      121 gaggatgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcattactga cgctcaggcg cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001345
VERSION     KHUT01001345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcagag gtgaaatccc ggagctcaac tccggaactg cctttgatac tggaggtctt
      121 gagtatggaa gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcttactggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001346
VERSION     KHUT01001346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 tacggaggtg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctttg
       61 caagtcagat gtgaaagccc cgggcttaac tcgggaactg catctgaaac tgcttagctt
      121 gagtcctgga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgc ggcgaaggcg gctacctgga cagtgactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001347
VERSION     KHUT01001347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaatgg catttgaaac tggcaggcta
      121 gagttcgata gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gcgacctgga tcagaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001348
VERSION     KHUT01001348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgac gtgaaatctc caagctcaac ttggaaatgg cgtcggatac tattcggctc
      121 gaggaatgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001349
VERSION     KHUT01001349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           95 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtttt
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaagtg ccgtggatac tggagacctc
      121 gaggagggta ggggcatctg gaacagccgg tggagcggtg aaatgcgttg atatcggctg
      181 gaactccgat ggcgaaggca aggtgctgga ccctctctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001350
VERSION     KHUT01001350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           82 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ctgggtgtaa agggtgctca ggcggacttg
       61 taagtcagag gtgaaactcc gaggctcaac ctcggaactg cctttgatac tgcaagtctc
      121 gagtacggga gagggtaatg gaataacagg tgtagcagtg aaatgcgtag atatctgtta
      181 gaacaccaat ggcgaaggca gttgcctggc ccgttactga cgctaaagca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001351
VERSION     KHUT01001351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcaccg
       61 caagtgtggg gtgaaatccc acggcttacc cgtggaactg cctggcagac tgcggtgcta
      121 gaggttggta ggggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gctctctggt ccaatcctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001352
VERSION     KHUT01001352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           77 g           64 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaatat
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaactg tcattgatac tggtattctt
      121 gaatgcagtt gaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactaaa ctgtgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001353
VERSION     KHUT01001353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacgaggggg gcaagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcggatgct
       61 taagttggat gtttaaaacc ggggcccaac cccggggcgg catccaaaac tgggcgtctt
      121 gaatggaaca gaggctagtg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccaat tgcgaaggca gctagctggg ttcacattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001354             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001354
VERSION     KHUT01001354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcatt
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatatgtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cggattattg acactgaggc acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01001355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001355
VERSION     KHUT01001355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           96 g           48 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggctttg
       61 taagtccgtt gtgaaatccc tcggctcaac cgaggaactg cgacggatac tgcttggctt
      121 gagtcccgga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gctacctgga cgggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001356
VERSION     KHUT01001356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          100 g           44 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttgga
       61 caagtcgtgt gttaaagccc ctggctcaac tgggggaggt catgcgagac tgtgcgactt
      121 gagggcaaga gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg acgtgctggg ttgcacctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001357
VERSION     KHUT01001357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           98 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agggtgcgta ggcggcaggg
       61 caagtgagga gtgaaagccc ctggctaaac taggggagga catctcagac tgcccagcta
      121 gagtgaaaca gaggagcgcg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggtgcaaacg gcgctctggg tttgaactga cgctgaggca cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001358
VERSION     KHUT01001358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           98 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggttcgt
       61 tatgtctgct gtgaaagccc cgggcttaac ccgggaatgg cagtggaaac tggcgggctt
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001359
VERSION     KHUT01001359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattca ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtccgat gtgaaatctg gaggctcaac ctccagcggt cattggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001360             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001360
VERSION     KHUT01001360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          102 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctttg
       61 gcagtcggtt agtgaaagcc cggggctcaa ccccggaggt gcggccgaaa caccaaggct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggacctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001361
VERSION     KHUT01001361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          100 g           39 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattca ttgggcgtaa agggcgcgca ggcggcccgg
       61 aaagtcggat gtgaaaactc ggggctcaac cccgagcgtg cacccgatac tgccgggctt
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01001362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001362
VERSION     KHUT01001362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          104 g           35 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgca ggcggcaggt
       61 caagtggcgg gtgaaagcgc ccggctcaac cgggcgagtg ccggtcagac tgacttgctg
      121 gagtaccggt gagggacgtg gaattccggg tgtagcggtg aaatgtgcag agatccggag
      181 gaacaccagc ggcgcaagcg gcgtcctgga cggaaactga cgctgagacg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001363             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001363
VERSION     KHUT01001363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          105 g           41 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agggtgcgca ggcggcggtt
       61 cgcgtggata gatgaaatct cctggcttac ctgggaggat ggtatccaga cgggatcgct
      121 tgagggtcgg agaggggcgt ggaattccgg gtggagcggt gaaatgcgta gagatccgga
      181 ggaacaccga cggcgtaggc agcgccctgg acgacacctg acgctgaggc acgaaagctg
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001364
VERSION     KHUT01001364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           94 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctcgg
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgccgagctg
      121 gagtcccgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg gccttctgga cggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001365
VERSION     KHUT01001365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           88 g           44 t
ORIGIN      
        1 tacagagact gcaagcgtta ctcggattca ctgggcgtaa agggagcgca ggcggaatcg
       61 tgtgtcggat gtgaaacccg ggggctcaac tcccggactg cgtctgaaac tgcgattcta
      121 gggactcgga ggggctagtg gaattcttgg tggagcagtg aaatgcgtag atatcaagag
      181 gaacaccaac ggcgaaggca gctagctgga cgagatccga cgctcaggct cgaaagtatg
      241 gggagcaaaa agg
//
LOCUS       KHUT01001366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001366
VERSION     KHUT01001366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggctcga
       61 tgcgtcgtgc gtgaaacacc ccggctcaac tggggagggt cgtgcgatac ggtcgagctt
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtactga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01001367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001367
VERSION     KHUT01001367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttggc
       61 aaagtcagct gtgaaagccc cgggcttaac ctgggaatgg cggttgaaac tagtcgacta
      121 gaatctggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001368
VERSION     KHUT01001368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggttgt
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tggcaaccta
      121 gagactggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccagcactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001369
VERSION     KHUT01001369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaatctc tgggcttaac ccagaaatgg cattggatac tattcggctt
      121 gaggtctgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cagatcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001370
VERSION     KHUT01001370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggacggg
       61 taagtatctt gtgaaatccc acggctcaac cgtggaactg ctggatatac tgcttgtctt
      121 gaggcactta ggggcagctg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaaa ggcgaaagca ggctgctggg agtgatctga cactgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001371
VERSION     KHUT01001371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcgggctta
       61 taagtcaggg gtgaaagcca ccggctcaac cggtgaactg cccttgatac tgtaagtctt
      121 gagtcctgga gaggctgtcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga ggcgtaagcg gacagctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001372
VERSION     KHUT01001372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           76 g           65 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaatag
       61 taagtcagtg gtgaaatctt gcggcttaac cgtaaaattg ccattgatac tgttattctt
      121 gagtatagtt gaagtgggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gctcactaag ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001373
VERSION     KHUT01001373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtc
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaactg cctgtgatac tggacgtctg
      121 gagtgaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gcatgctgga cctcaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001374
VERSION     KHUT01001374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           82 g           54 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggttatt
       61 caagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgaaac tagataactt
      121 gaatcacgga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactgga cgtgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001375
VERSION     KHUT01001375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           84 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctca
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgcgaggctt
      121 gagtgaagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactaga cttcaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001376             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001376
VERSION     KHUT01001376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctttg
       61 taagtttggt gtttaatctc gaagctcaac ttcgattcgc atcgaaaact gcaaggcttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001377
VERSION     KHUT01001377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctct
       61 ttagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tgggaggcta
      121 gagttcggga gagggatgta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgaaactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001378
VERSION     KHUT01001378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           82 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctta
       61 taagtcagtg ctgaaatact ctagcttaac tagaggggtg gcattgatac tgcaaggctt
      121 gagtacagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001379
VERSION     KHUT01001379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           56 c           89 g           41 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggtatgg
       61 caagttggat gtgaaatctc ccggctcaac tgggagaggc cgttcaatac tgccaaacta
      121 gagggcgaca gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaga ggcgaaggcg acctcctggg tcgcacctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001380
VERSION     KHUT01001380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           95 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggcgatt
       61 ttagtcaggc gtgaaagccc tgggcttaac ctaggaactg cgtttgatac tggattgctt
      121 gagtgtggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001381
VERSION     KHUT01001381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           91 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtcctg
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaagac tgcagggctt
      121 gagtatggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001382
VERSION     KHUT01001382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 taagttcgtt gtgaaagccc cgggctcaac ctgggaactg caatggaaac tggcgggctt
      121 gaatgcggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgacattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001383
VERSION     KHUT01001383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggctgt
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tatcagtctt
      121 gaattttgtt gaggtaggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcttactaaa caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001384
VERSION     KHUT01001384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          100 g           42 t
ORIGIN      
        1 tacgaaggtc ccgagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 caagtcggat gtgaaagccc ggggctcaac cccggaatgg cattggatac tgccgtgctg
      121 gagcggtgga gagggatctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaaggctcgt ggcgaaggcg agatcctgga cactgactga cgctgaggca cgaaggccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01001385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001385
VERSION     KHUT01001385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           98 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agagtgcgta ggcggcatcg
       61 taagtgggac gtgaaagcct ctggctaaac taggggagga cgactcagac tgcgaagcta
      121 gaatacagta gaggactgcg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccaga ggtggagacg gcggtctggg ctgtgattga cgctgaggca cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001386
VERSION     KHUT01001386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           80 g           57 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactag
       61 taagtcagtg gtgaaagccc atcgcttaac ggtggaactg ccattgatac tgctagtctt
      121 gagtatggtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgaccca
      181 gaacaccaat tgcgaaggca gctcgctaag ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001387
VERSION     KHUT01001387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agcgtgcgta ggtggttcgg
       61 taagacctgt gcgaaatccc tgggcttaac ccaggaaggt cgcaggtgac tgccgtgctt
      121 gaggacggga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaaggccggt ggcgaaggcg gctctctgga acgttcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001388
VERSION     KHUT01001388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcttct gtgaaatctc caggctcaac ctggagcggt caggggaaac tgccgtgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001389
VERSION     KHUT01001389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          101 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccggg
       61 ttggtcgtgg gtgaaatccc ccagcttaac tggggaattg cctacgatac tgcctggctc
      121 gagggaggaa ggggcggccg gaactgtcgg tggagcggtg aaatgcgttg agatcgacag
      181 gaacgccggt ggcgaaagcg ggccgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001390
VERSION     KHUT01001390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           92 g           52 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggcgcgg
       61 tatgtctctt gtgaaatctc caggctcaac ttggagtctg caagggaaac tgccgtgctt
      121 gagtactgga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga cagtaactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001391
VERSION     KHUT01001391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaactg cattggatac tggcgatctg
      121 gagtgcggta gagggtggcg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatccgt ggcgaaggcg gccacctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001392
VERSION     KHUT01001392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtagg
       61 taagtcagtg gtgaaatccc cgagcttaac tcgggaactg ccgttgatac tatctatctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactaca cgatcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001393
VERSION     KHUT01001393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           83 g           54 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtaccg
       61 aaagttaggt gtgaaagttc gaggctcaac ctcgaaattg cacctaatac tccggtgctt
      121 gagtctcata gaggaatctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgaa
      181 gaaggcctgt ggcgaaagca agattctggg tgacgactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001394
VERSION     KHUT01001394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           48 c          102 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtctgct gtgaaagccc ggggcttaac tccggagggt cggtggatac tgccagtctt
      121 gaaggtgcta gggagagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcttggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001395
VERSION     KHUT01001395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           92 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cacgtcactt gtggaaaacc ggggcttaac tccggtcggg caggtgatac gggcttactt
      121 gagtgctgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca ggtctctggg cagttactga cgctgaggag cgaaagcatg
      241 ggtatcgaac agg
//
LOCUS       KHUT01001396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001396
VERSION     KHUT01001396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcaaacgttg tccggattca ctgggcgtaa agcgcgcgca ggcggattgg
       61 tcagtccgag gttaaagctc ccggctcaac tgggagaggc ccttggatac tgccagtctt
      121 gaggtaccta ggggagagtg gaattccggg tgtagtggtg gaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg actctctggg ggtgacctga cgctgaggcg cgaaagcatg
      241 gggagcaaac cgg
//
LOCUS       KHUT01001397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001397
VERSION     KHUT01001397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           46 c           96 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgact gtgaaatctg agggcttaac tttcagattg cagtcgatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001398
VERSION     KHUT01001398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttac
       61 taagtcaggt gtgaaatccc cgggctcaac ctgggaactg cgcttgatac tggtaaacta
      121 gagtttagta gagggaagtg gaattccgcg tgtagcggtg aaatgcgtag atatgcggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctaaaactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001399
VERSION     KHUT01001399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtataa agggtgcgta ggcggcttgt
       61 taagtcagga gtgaaaggcc atggctcaac tatgggattg cttttgatac tgacgagctt
      121 ggattaggac gaggttggcg gaatgtggca agtagcggtg aaatgcatag atatgccata
      181 gaataccgat tgcgaaggca gctgactaga cctatattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001400
VERSION     KHUT01001400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccgt
       61 cacgtcggga gtgaaaactc aaggctcaac cttgagcgtg cttccgatac gggcggacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01001401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001401
VERSION     KHUT01001401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           98 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggccagg
       61 taagtccgtt ctgaaagccc ggggctcaac cccgggaggc ggatggatac tgcttggctc
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001402
VERSION     KHUT01001402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           92 g           45 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcggatgtt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggacatctc
      121 gaggcactca ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaagaca gctcgctggg ggtgtcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001403
VERSION     KHUT01001403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taggtccgtt gtgaaaactc gaggctcaac ctcgagacgc cgatggaaac catttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggctaaggcg gctctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001404
VERSION     KHUT01001404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgtc
       61 cacgtcggat gtgaaagctc ggggctcaac cctgagaggc cattcgatac gggacggcta
      121 gaggcaggta ggggaaagtg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctttctggg ccttgcctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01001405             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001405
VERSION     KHUT01001405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          101 g           47 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggccagg
       61 taagtctgtc tgtgaaagcc cggggctcaa ccccgtggat tgcaggggat actgcctggc
      121 tggagacaag cagaggttgg tagaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aggaatacca gaggcgaagg cggccaactg ggcttgttct gacgctgagg tgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01001406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001406
VERSION     KHUT01001406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           43 c           94 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggttgtc
       61 taagtttggt gtgaaatctc ctggcttaac tgggagggtg cgccgaaaac tgggtgactg
      121 gagtgtcgga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga cgataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001407             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001407
VERSION     KHUT01001407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           85 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgca ggcggattga
       61 taagtcaggg gtgaaatccc ggagctcaac ttcggaattg cctttgatac tgtctatctt
      121 cgagttcggg agaggttggc ggaattccta gtgtagaggt gaaattcgta gatattagga
      181 agaacaccag tggcgaaggc ggccaactgg cccgatactg acgctcatgt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001408
VERSION     KHUT01001408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           83 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggaatca ctgggcgtaa agggtgcgta ggctggcttc
       61 taagtcagat gtgaaatcca atggcttaac cattgaactg catttgaaac tgggagccta
      121 gagttcagaa ggggcagatg gaattagtgg tgtaggggta aaatccgtag atatcactag
      181 gaatatcaaa agcgaaggcg atctgctggg atgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001409
VERSION     KHUT01001409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcgctt
       61 taagtcaggg gtgaaatacg gcagcttaac tgtcgcagtg cctttgatac tgaagtgctt
      121 gaatgcggtt gaagacggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccaat tgcgaaggca gctgtctaag ccgatattga cgctgatgca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001410
VERSION     KHUT01001410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggtccag
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tactggactt
      121 gaatactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001411             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001411
VERSION     KHUT01001411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          102 g           43 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgcga
       61 taagttgggc gtgaaaggcc cgggctcaac ccggggactg cgcttgatac tgtcgcgctg
      121 tgagtccggg agaggatggc ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggccatctgg accggcactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001412
VERSION     KHUT01001412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           41 c           80 g           54 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agggtctgta ggtggttttc
       61 taagtctgct gttaaagctt gaagctcaac ttcaaaaatg catcggaaac tagaagactt
      121 gagtatggta ggggtagagg gaatttccag tgaagtggtg aaatgcgtag atattggaaa
      181 gaccaccaaa ggcgaaggca ctctactggg ccacaactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001413
VERSION     KHUT01001413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           97 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcg
       61 caagtctgtg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tgcgaatctt
      121 gaggagggta ggggcatctg gaacggtcgg tggagcggtg aaatgcgttg atatcgaccg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001414
VERSION     KHUT01001414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggactcg
       61 taagtcagtg gtgaaatctc atagctcaac tatgaaactg ccattgatac tgcgggtctt
      121 gagtaaggta gaagtggctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggtcactatg tcttaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001415
VERSION     KHUT01001415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctcga
       61 taagtctgtt gtgaaagtca aaggctcaac ctttgaatgc cgatggatac tgtcgagcta
      121 gagtccggaa gaggcgggtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gcccgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001416
VERSION     KHUT01001416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctatt
       61 taagtcaggg gtgaaagccc ggagctcaac tccggaactg cctttgagac tggatagcta
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001417
VERSION     KHUT01001417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           83 g           52 t
ORIGIN      
        1 gacgagcctc acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgtgg
       61 aaagtcggat gtgaaatacc tcagcttaac tgaggaactg cactcgatac ttccatgcta
      121 gagtaagaca gaggtgagtg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaggcg actcactggg tctttactga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001418
VERSION     KHUT01001418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agagcacgta ggtggttcgc
       61 taagtcacgt gtgaaatccc ccggctcaac cggggaattg cgcgtgatac tgacgaactc
      121 gaggcgggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga cccgatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001419
VERSION     KHUT01001419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c           99 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtctatc gtgaaaacct ggggcttaac cccgggcctg cggtggatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001420
VERSION     KHUT01001420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcgc
       61 taagtcggat gtgaaaactc tgggctcaac ccagagcctg cattcgaaac tgacgagctc
      121 gagttctgga ggggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cagaaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001421
VERSION     KHUT01001421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatta ctgggcgtaa agggtgcgca ggcggctcgg
       61 ttagtggaat gtgaaatgcc ctggcttaac cagggaactg cattccatac tgccgggctt
      121 gagtatagga gaggatgggg gaattcccgg tgtagcggtg gaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggc ctaatactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001422
VERSION     KHUT01001422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           61 c           85 g           45 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gcttgttctg
       61 caagtcttcc gttaaatcca cctgctcaac agatgggctg cgggagatac tacagggcta
      121 ggaggcggga gaggcaagcg gtactcagtg ggtaggggta aaatcctctg atccactgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01001423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001423
VERSION     KHUT01001423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           48 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac tactgtgctt
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001424
VERSION     KHUT01001424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          102 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcctgt
       61 tgcgtctgcc gtgaaagccc gtggcttaac tgcgggtctg cggtggatac gggcaggcta
      121 gaggctggta ggggcaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttgctggg ccagttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001425
VERSION     KHUT01001425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggttagg
       61 taggtccgtt gtgaaaactc gaggctcaac ctcgagacgc cgatggaaac cccctaacta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggctaaggcg gctctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001426
VERSION     KHUT01001426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtccgac gtgaaatccc caggcttaac ctgggaactg cgtcggatac tggatggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgatgag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001427
VERSION     KHUT01001427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           94 g           43 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggaatgt
       61 caagttaggg gtgaaaggcc tgggctcaac ctaggaacgg cctctaaaac tgacaatcta
      121 gagtccggaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggt ccggcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001428
VERSION     KHUT01001428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c          101 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 taagtccgct gtgaaagccc ggagcttaac tccggagggt cggtggatac tgccagtctt
      121 gaaggtgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001429
VERSION     KHUT01001429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 tacgtaccgg acgaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggccttg
       61 aaggtggggt gtgaaagccc acggctcaac cgtggaattg cgctccaaac cgcaaggctt
      121 gagggagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacacccgt ggcgaaggcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001430
VERSION     KHUT01001430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           93 g           52 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggctctt
       61 caagtctgac gtgaaatctc tcggcttaac cgggagcggt cgttggaaac tgaagggctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaagcg gcaccctggc attagcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001431
VERSION     KHUT01001431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           82 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatccc cgggcttaac ttgggaactg ccgttgatac tatctgtctt
      121 gaatatcgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctcactaca cggtaattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001432
VERSION     KHUT01001432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           91 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatctga
       61 aaagttctgt gtgaaagccc acatgttaac tgtggaagtg cacagaaaac tgttggattt
      121 gagttcagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001433
VERSION     KHUT01001433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           89 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgattttg
       61 taagtcttgt gtgaaagccc aaagcctaac tttggaagtg cacaagaaac tgcgagaata
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001434
VERSION     KHUT01001434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           96 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 tgcgtcggga gtgaaaactc ggagcttaac tccgagcttg cttccgatac gggcagacta
      121 gaggtaggca ggggagagcg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctctctggg cctattctga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001435
VERSION     KHUT01001435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggttcga
       61 taagttgggg gtgaaatccc tgggctcaac ccaggaatgg ccttcaagac tgtcgatcta
      121 gaggacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001436             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001436
VERSION     KHUT01001436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c          100 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccact
       61 taagtccgta ggtgaaagac cggggctcaa ccccgcgtca gccatggata ctgggaggct
      121 ggagacaggt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gatatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg acctggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001437
VERSION     KHUT01001437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           96 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacag
       61 caagtcggga gtgaaagccc ggggctcaac cccggaatgt ctttcgaaac tgctgagcta
      121 gagtacggaa ggggcaaccg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001438
VERSION     KHUT01001438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctgtt gtgaaaactc gaggctcaac ctcgggcttg cagtggatac gggcagacta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccgcaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01001439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001439
VERSION     KHUT01001439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgttg tccggaatca ttgggcgtaa agggtcctga ggtggtttgc
       61 tcagtccact gtgaaagttc agggcttacc cttgaaaatg cggtggaaac tagtaggcta
      121 gagtacggtg ggggcagagg gaattcccga tgtagcggtg aaatgcatag atatcgggaa
      181 gaacaccgat ggcgaaggca ctctgctggg ccgacactga cactgaaaga cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001440             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001440
VERSION     KHUT01001440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           44 t
ORIGIN      
        1 tacgagggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccacg
       61 acaagtcact tgtgaaatct ccgggcttaa cccggaaagg tcaagtgata ctgtcgggct
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001441
VERSION     KHUT01001441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           56 c          103 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgca ggcggctcgg
       61 cacgtccgtt gtgaaatcgc ccggctcaac tgggcggggt cagcggatac ggccgagctg
      121 gagcaagcta gggggcaatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctgga gctttgctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01001442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001442
VERSION     KHUT01001442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           74 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc cgtgcttaac atggaaactg ccattgatac tactgatctt
      121 gaattttcag gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca ggaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001443
VERSION     KHUT01001443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggattta ctgggcgtaa agcgcgtgta ggcgggaaga
       61 aaagtcgggt gtgaaatccc tgggctcaac ctaggaactg cattcgaaac tttctttctg
      121 gagtgatgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc catcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001444
VERSION     KHUT01001444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagtctgtt gtgaaagccc tgggctcaac ctgggaatgg caatggaaac tggcgagcta
      121 gagtcaggta gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccacctgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001445             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001445
VERSION     KHUT01001445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           52 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcttga
       61 taagtgtgat gtgaaatctc cagggcttaa cccggaaact gcaccgcaga ctgtcaggct
      121 tgaggaatgc agaggtaact ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc aggttactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001446             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001446
VERSION     KHUT01001446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           47 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 atagtcgggt gtgaaaactc cgagctcaac tcggagacgc cacccgatac tgccatgact
      121 agagtccggt aggggagcag ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gctgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001447
VERSION     KHUT01001447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           91 g           43 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggcccga
       61 taagttaggt gtgaaacctc ccggctcaac tgggagagga cgcttaagac tgtcgggctc
      121 gagggtggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccacatctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001448
VERSION     KHUT01001448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           85 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtccca
       61 aaagttaggt gtgaaatccc tcggctcaac cgaggaactg cgcttaaaac tgtgggactc
      121 gagggagata gaggaaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctttctggg tcttacctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001449
VERSION     KHUT01001449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           96 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctgt
       61 caagtcatat gtgaaagccc acggctcaac cgtggaagtg catgtgaaac tggcaggctt
      121 gagtactgga ggggagtgtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg acactctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001450
VERSION     KHUT01001450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacggggggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttcg
       61 caagtcaaag gtgaaatccc tcggctcaac cgaggaattg cctttgaaac tgcgtggctt
      121 gaggccggga gggggcagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctgcctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001451
VERSION     KHUT01001451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          102 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggccccg
       61 tatgtgcttg gtgacatcct cccgcttaac gggaggcggc ccaggtagac agcggagcta
      121 gaggtaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctaaactga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001452
VERSION     KHUT01001452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           90 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcttgt
       61 taagtccggg gtgaaagccc acagctcaac tgtggaactg ccctggatac tggcgagctt
      121 gagtccagac gaggttggcg gaatggatgc tgtagcggtg aaatgcatag atagcatcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001453
VERSION     KHUT01001453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgtgca ggcggtctgt
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaatgg cgcttgttac tggcagacta
      121 gagtgcgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg atgacactga cgctcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001454
VERSION     KHUT01001454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           92 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctgcc
       61 taagtcaggt gtgaaatctc tcggctcaac cgggaaactg cgcttgatac tgggcggctc
      121 gagtgaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgtgtag atatcggaag
      181 gaacgccagc agcgaaagcg gcacactggg ccttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001455             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001455
VERSION     KHUT01001455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcggac gtgaaatctc cacgctcaac gtggaggggc cgttcgatac tgtcgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001456
VERSION     KHUT01001456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 caagtcaatg gtgaaagccc gaggctcaac ttcgggtcgg ccactgatac tgttgggctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001457
VERSION     KHUT01001457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           39 c           84 g           62 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctgtt
       61 taagtcagtg gtgaaatacg gttgctcaac aatcgaggtg ccattgatac tgaatagctt
      121 gaaataattg gaggctgccg gaatggatgg tgtagcggtg aaatgcatag atatcatcca
      181 gaacaccgat tgcgaaggca ggtggctacg atttgtttga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001458             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001458
VERSION     KHUT01001458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          101 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccctt
       61 taagtctacc cgtgaaatgc cggagctcaa ctccgtgcac gccgggggat actggagggc
      121 tggagacagg tagaggcagg cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacacca gaggcgaagg cggcctgctg ggccttgtct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01001459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001459
VERSION     KHUT01001459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           61 c          109 g           37 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggtggtccat
       61 taagcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac tggtggactt
      121 gagggcggca ggggctggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgaccctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001460
VERSION     KHUT01001460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           98 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaagccc agggcttaac cctgggtctg cagtcgatac gggcaggcta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001461
VERSION     KHUT01001461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           75 g           62 t
ORIGIN      
        1 tacgtaggtc ccaagcgtta tccggaatta ctgggcgtaa agagtctgta gctggtctcg
       61 aaagtctact ttcaaatacc ttggcttaac caaggaaagg gggtggatac tacgagtctg
      121 gaagttttca ggggtaagta gaatttccgg tggagaggtg aaattcgttg atatcggaag
      181 gaatgccaaa agcgaaagca gcttactatg aatatcttga cagtgagaga cgaaagtttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001462
VERSION     KHUT01001462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgc
       61 tgcgtccgct gtgaaaatcc agggctcaac cctggacttg cagtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001463
VERSION     KHUT01001463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 gacgtagggg gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggattcg
       61 taagttgagg ttgaaagatc cgggctcaac tcggggcacg gcctcaatac tgcgaacctt
      121 gagacaagta gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggg cttgttctga cgctgaggca caaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001464
VERSION     KHUT01001464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           91 g           48 t
ORIGIN      
        1 gacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggctgtt
       61 caagttgggc gtgaaagcct tgggcttaac ccaagaaatg cgctcaatac tggatagcta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001465
VERSION     KHUT01001465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctatt
       61 cacgtcggat gtgaaagctc aaggctcaac cttgagaggc cattcgatac gggatagcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gatctctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01001466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001466
VERSION     KHUT01001466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacggggggg gtaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcggac gtgaaatccc tcagcttaac tggggaactg cgtccgatac tggatggctt
      121 ggattcggga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccgacattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001467
VERSION     KHUT01001467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           47 c          102 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatttg
       61 tcagtcgggc gtgaaattcc tgggcttaac ctgggggctg cgttcgagac ggcgggtctt
      121 gagtttggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt cctggactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001468
VERSION     KHUT01001468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           90 g           49 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 caagtcttgt gtgaaagccc agggcttaac tctggaagtg cacaagaaac tgttgaattt
      121 gagtacagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001469
VERSION     KHUT01001469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           59 c          112 g           36 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggcggtg
       61 tcagcccgac gtgaaagccc ccggcttaac cggggagggt cgtgggggac ggcaccgctt
      121 gagggcggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccgactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001470
VERSION     KHUT01001470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c          102 g           38 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggtctcg
       61 caagtctggc gtgaaagccc aaggctcagc cttggaagtg cgctcgaaac ggcgaggctg
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001471
VERSION     KHUT01001471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcctga
       61 taagtacggg gtgaaaccct gcagcttaac tgcaggacgg cctcgtagac tgtccggctt
      121 gagcatggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001472
VERSION     KHUT01001472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c           99 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgtcc gtgaaaaccc acagctcaac tgtgggcttg cggtcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001473
VERSION     KHUT01001473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           94 g           51 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgtacgca ggcggcttcg
       61 ttagtcggat gttaaagctc ctggcttaac tgggagaggc cattcgatac tgcggggctt
      121 gaggttggga gaggggtatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtaccctggc ccacacctga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001474
VERSION     KHUT01001474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaacg
       61 caagtcggat gtgaaagccc atggctcaac catggaagtg cactcgaaac tgcgttgcta
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgagtactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001475
VERSION     KHUT01001475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagtcagct gtgaaagccc cgggctcaac ctgggaactg cagttgatac tggccgacta
      121 gagtacgaga gagggaggta gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaataccagt ggcgaaggcg gcctcctggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001476
VERSION     KHUT01001476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggttgtg
       61 tgtgtcaggt gtgaaatctc ggagcttaac tccgaaactg cgcctgaaac tacatgacta
      121 gagcattgga gagggtagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01001477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001477
VERSION     KHUT01001477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           90 g           47 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggctaag
       61 taagtcggat gttaaatccc caggctcaac ctgggacacc acccgatact gctttggcta
      121 gagtccggta ggggagcgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagc ggcgaaggcg gcgctctggg ccggtactga cactgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001478
VERSION     KHUT01001478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           43 c           79 g           52 t
ORIGIN      
        1 gacggggggg gctagtgtta ttcggaatga ctaggcgtaa agggcacgta ggcggtgaat
       61 ccggttgaaa gtgaaagtcg ccagctcaac tggcggaatg ctttcaaaac caattcactt
      121 gagtaagata gaggatagtg gaatttcgtg tgtagagata aaattcacag atatacgaag
      181 gaacaccaaa agcgaaggca gctttctggg tctctactga cgctgaggtg cgaaagcata
      241 gggagcaaac agg
//
LOCUS       KHUT01001479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001479
VERSION     KHUT01001479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           86 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcgcca
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgtggtgctt
      121 gagtgtagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gcttactagg ctacaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001480
VERSION     KHUT01001480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           93 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaggg
       61 caagtcggat gtgaaagccc acggctcaac cgtggaagtg cacccgaaac tgccccgcta
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001481             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001481
VERSION     KHUT01001481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          101 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggactg
       61 tcaagtcggg agtgaaatct cggggctcaa ctccgaggct gcttccgaaa ctgcggacct
      121 ggagaccggt agaggtgggt ggaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacacctg tggcgaaggc ggcccactgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001482             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001482
VERSION     KHUT01001482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           99 g           41 t
ORIGIN      
        1 tacgtagggg gcgaacgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcggcc gtgaaagccc ggggctcaac cccgggatgc cggtcgaaac tgctgtggct
      121 agggtccggt agaggagagt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggctctctgg gccggtaccg acgctgagga gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001483
VERSION     KHUT01001483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcgatg
       61 taagtcggac gtgaaagccc tcggcttaac cgaggaagtg cgtccgatac tgtgtcgctt
      121 gagtaccgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001484
VERSION     KHUT01001484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          102 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctga
       61 aaagtccggg gtgaaatccc cccgctcaac gggggaacgg cctcggatac tgtcggcctg
      121 gagggaggta ggggcgagcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gctcgctgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001485
VERSION     KHUT01001485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctttg
       61 tcagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tgcaaggctt
      121 gagaccggaa gaggtcagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctgactggt ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001486
VERSION     KHUT01001486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcatgca ggcggtcggc
       61 taagatgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tggctgactc
      121 gaggatggta ggggaaagtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccactcctga cgctcagatg cgacagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001487             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001487
VERSION     KHUT01001487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          107 g           34 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccgc
       61 acaagtctgc ggtgaaaacc cccggcttaa ccgggggccg gccgtggaaa ctgttgggct
      121 ggaggctggg agagggcagc ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 ggaacaccgg cggcgaaggc ggctgcctgg accagtcctg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001488             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001488
VERSION     KHUT01001488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           63 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtcgg
       61 tgcgtcggcc gctgaaatcc cccggctcaa ccggggaagg ggcgccgata cgaccggcct
      121 ggagcgacgt agggggaact ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagttcctgg acgtcggctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001489
VERSION     KHUT01001489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           81 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctatc
       61 taagtcagtg gtgaaatatc tcagcttaac tgagagggtg ccattgatac tggatagctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttaccaag ctgtaactga cactgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001490
VERSION     KHUT01001490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggatctg
       61 taagtcggga gtgaaatctc ggggctcaac cccgagcctg cttccgaaac tgcaagtctt
      121 gaggactgga gaggaaagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggag
      181 gaacatcggt ggcgaaggcg actttctgga cagttcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001491
VERSION     KHUT01001491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggccact
       61 taagtcggat gtgaaagccc ctggctcaac tggggaagtg cacccgaaac tgagcggctt
      121 gaatacaaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg ttgatattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001492
VERSION     KHUT01001492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           82 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccgttgatac tatctgtctt
      121 gaatatcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag agatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cggtaattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001493             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001493
VERSION     KHUT01001493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttac
       61 aaagtcaggg gtgaaatctt gcggcttaac cgtaatcagc ctctgatact ggtaggctcg
      121 agggaggtag gggtatgtgg aacttctggt ggagcggtga aatgcgttga tatcagaagg
      181 aacgccggtg gcgaaagcga cgtactggac ctcttctgac gctgaggcgc gaaagctagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01001494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001494
VERSION     KHUT01001494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           89 g           51 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgattgaa
       61 taagtctcgt gtgaaagccc agagcttaac tctggaagtg cacgagatac tgtttaattt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgttgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001495
VERSION     KHUT01001495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggttaga
       61 taggtctgct gtgaaaactc caggctcaac ctggagacgt cggtggaaac catctagcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001496
VERSION     KHUT01001496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           85 g           56 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ctgggtgtaa agggcgcgca ggtggatctg
       61 caagtcaggg gtgaaatctc acggcttaac cgtgaaagtg cctttgatac tgcagatctt
      121 gagttcggaa gagagagacg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt tgcgaaggcg gtctcttggt ccgatactga cactcaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001497
VERSION     KHUT01001497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgag
       61 caagtcgaat gtgaaatccc ccggctcaac cggggacttg catccgatac tgcctggctt
      121 gagtcctgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga caggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001498
VERSION     KHUT01001498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaagact ccggctcaac cggaggcccg ccattgatac tgcagggctt
      121 gagtgcagat gaggccggcg gaatttgtgg tgtagcggtg aaatgcatag ataccacaaa
      181 gaacaccgat tgcgaaggca gctggccaag gtgcaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001499
VERSION     KHUT01001499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           97 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggttgtg
       61 ggcgtcggtg ctgaaagtcc actgcttaac agtgggatgc gtgccgatac gccataactg
      121 gagcgatgca gaggccagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca gctggctggg catttgctga cgctcatgtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01001500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001500
VERSION     KHUT01001500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           94 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcttt
       61 taagtttgac gtgaaagctc tcggcttaac tgggagaggt cgttgaagac tggaaggctt
      121 gaggcaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc gttagcctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001501
VERSION     KHUT01001501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggattcc
       61 taagtcgggg gtgaaatcct ggagcttaac tccagaactg ccttcgatac tgggaatctt
      121 gagtccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001502
VERSION     KHUT01001502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           85 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtgaggc gtgaaagcct ggagcttaac tccagaattg cacctcaaac tacatggcta
      121 gagcattgga gagggtagca gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaataccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01001503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001503
VERSION     KHUT01001503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           92 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggttatt
       61 caagtcgaat gtgaaagccc ccggcttaac tggggagggt cattcgatac tgtttaactt
      121 gaaggcagga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ctgttcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001504
VERSION     KHUT01001504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           74 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggatatg
       61 taagtccgtg gtgaaatctc taagcttaac ttagaaactg ccctggatac tatatgtctt
      121 gaatgttgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctggctacc caactattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001505
VERSION     KHUT01001505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggtggtctgt
       61 tatgtcagat gtgaaagccc tcggcttaac tgaggaagtg cacctgaaac tggcaggctt
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggt tcgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001506
VERSION     KHUT01001506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcagtg gtgaaagcca acagctcaac tgttgaactg ccattgatac tgataggctt
      121 gagtacagac gaggtaggcg gaattgatgc tgtagcggtg aaatgcatag atagcatcaa
      181 gaacaccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001507
VERSION     KHUT01001507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           80 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcctt
       61 taagtcagtg gtgaaatctc cccgctcaac ggggaaactg ccattgatac tattggtctt
      121 gaatttagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001508
VERSION     KHUT01001508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           90 g           48 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcttta
       61 taagtgaggt gttaaatcct cgggctcaac ccgagaaccg cacttcatac tgttaagctt
      121 gagtgcagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg ctgttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001509
VERSION     KHUT01001509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctgat gttaaagcct ggcgctcaac gccagcatgg cattggaaac tgggcagcta
      121 gagtgtggta gaggtcacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtgactggg ccacaactga cgctgaggaa cgaaagctag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01001510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001510
VERSION     KHUT01001510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           96 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctgcg
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgcgtagctg
      121 gagtctcgga gagggaggcg gaattctggg tgtagaggtg aaattcgtag atatccagag
      181 gaacaccggt ggcgaaggcg gcctcctgga cgagaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001511
VERSION     KHUT01001511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           98 g           47 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctatt
       61 tttgtcaggc gtgaaagccc cgggcttaac ctgggaactg cgcttgagac gggatggcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001512
VERSION     KHUT01001512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcggactcg
       61 taagtcagag gtgaaagccc gcggctcaac cgcggaactg cctttgatac tgcgagcctt
      121 gagtcctgga ggggttggcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga ggtgtagacg gccaactgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001513
VERSION     KHUT01001513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtttct
       61 taagtctgat gtgaaagccc acggcttaac cgtggagggt cattggaaac tgggagactt
      121 gagtgcagaa gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgcaactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001514
VERSION     KHUT01001514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           89 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcttc
       61 taagtttgat gtgaaatccc ggggctcaac cctggaattg cattgaatac tggaggacta
      121 gagtaccgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cggtaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001515
VERSION     KHUT01001515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgtctt gtgaaatccc accgctcaac ggtggaactg ctgggcatac tggcgatctt
      121 gagccaccta ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001516
VERSION     KHUT01001516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgagg
       61 taagtctgat gtgaaatctc cgagctcaac tcggaaactg catcggatac tattttgctc
      121 gagggttgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001517
VERSION     KHUT01001517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatagg
       61 taagtccgtg gtgaaatcct gcagcttaac tgcagaactg ccatggatac tatctatctt
      121 gaatgtcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctaactacg cgataattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001518
VERSION     KHUT01001518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggccgag
       61 caggtctgtt gtgaaaactg gaggctcaac cttcagacgt cgacagaaac cgcgcggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001519
VERSION     KHUT01001519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           99 g           43 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaataa ctgggcgtaa agggtccgta ggcggctgcg
       61 caggtctggc gtgaaagctc tcggctaaac tgagagagtg cgttggaaac cgcgtagcta
      121 gcatcacgca ggggctggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctctag agcgaaggca accggctagg cgatgatgga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001520
VERSION     KHUT01001520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           87 g           56 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatta ttgggcgtaa agggtctgta ggcggatgtt
       61 taggtctgat gtgacaattc ttggcttaac tgagaaagtg cattggaaac cggacaacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001521
VERSION     KHUT01001521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          101 g           36 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggacgcg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcgcgtctg
      121 gagccatgca gagggcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgcctggg catgcgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01001522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001522
VERSION     KHUT01001522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcctga
       61 taagtcggct gtgaaagtca agggctcaac ccttgaatgc cggccgatac tgtcaggcta
      121 gaatccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctggg acggtattga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001523
VERSION     KHUT01001523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcttgg
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaatgg cattgaatac tattgagctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001524
VERSION     KHUT01001524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttt
       61 caagtcagac gtgaaagctc tgggcttaac ccagaaattg cgtttgatac tgttaagcta
      121 gagtgtggaa ggggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccaacactga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001525
VERSION     KHUT01001525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggtggtttcg
       61 taagtccgtt gtgaaagtca aaggctcaac ctttgaaagc cgatggatac tgcggagcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001526
VERSION     KHUT01001526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttga
       61 taagcgagat gtgaaagccc cgggcttaac ctgggaacgg catttcgaac tgtcaggcta
      121 gagtgtggta gagggtagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ccaacactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001527
VERSION     KHUT01001527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           98 g           47 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggactgt
       61 tgcgtccgct gtgaaaagct ggggcttaac cctggccttg cagtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001528
VERSION     KHUT01001528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           92 g           44 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtcggat gtgaaatctg gaggctcaac ctccagctgt catccgaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001529
VERSION     KHUT01001529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggtggtctgt
       61 tacgtctgct gtgaaaacct agggcttaac cctgggcctg cagtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cactgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001530             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001530
VERSION     KHUT01001530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcggcggcg
       61 taagttggaa gtgaaatccc atcggctcaa ccgtggaata gctttcagta ctgcgtcgct
      121 cgagtgagat aggggtccgt ggaactaccg gtggagcggt gaaatgtgtt gatatcggtg
      181 ggaacgccag tggcgaaagc gacggactgg acctttactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001531
VERSION     KHUT01001531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           83 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatact gcagcttaac tgtagcattg ccattgatac tgtagggctt
      121 gagtacagat gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag ataccatcaa
      181 gaacaccgat tgcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001532
VERSION     KHUT01001532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           40 c           88 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggaactg
       61 taagtctgat ttgaaagcaa gcggcttaac cgtttgatgt ggttggaaac tgtggttctt
      121 gaatgggttg gcggtagccg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001533
VERSION     KHUT01001533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           96 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgttc
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tgggacgcta
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001534
VERSION     KHUT01001534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           97 g           48 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggccgat
       61 taagtttgtc gtgaaatctc ccggctcaac tgggagggtg cggcggaaac tgatgggctc
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgcaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001535
VERSION     KHUT01001535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgagac tgcatcgctt
      121 gaatcatgga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001536
VERSION     KHUT01001536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacggaatgt gcaaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggcatgt
       61 taggtgaggt gtgaaatccc ccggctcaac cgaggaattg cgcttcaaac cggcgtgctt
      121 gaggaagata gaggtgggcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gcccactggg tcttttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001537
VERSION     KHUT01001537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           83 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtccca
       61 taagtcagtg gtgaaatact ctagcttaac tagaggggtg ccattgatac tgcgggactt
      121 gagtggagtt gaagtgggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gctcactaag ctcaaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001538
VERSION     KHUT01001538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacttt
       61 taagtcgggg gtgaaagccc agggctcaac cctggaattg ccttcgatac tgagagtctt
      121 gagttcggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001539
VERSION     KHUT01001539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           61 c          109 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggctggt
       61 caagcccgac gtgaaagccc ccggctcaac cggggagggt cgtcggggac tgatcggctt
      121 gagggcggta ggggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt agcgaaggcg gccggctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001540
VERSION     KHUT01001540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           77 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaatccc ggggctcaac ctcggaactg ccattgatac tattagtctt
      121 gaattatgtt gaggttggcg gaatataaca tgtagcggtg aaatgcttag atatgttatg
      181 gaacaccgat tgcgaaggca gctggctaaa catatattga cgctgatgca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001541
VERSION     KHUT01001541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggtgcgta ggcggtttag
       61 tgggtgtggt gtgaaagccc cgggcttaac ctgggaagtg caccgcaaac tattagacta
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001542
VERSION     KHUT01001542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcatt
       61 taagtcagtg gtgaaatacc tgagcttaac ttgggaactg ccattgatac tatttgtctt
      121 gaatgtcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gctaactacg cgaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001543
VERSION     KHUT01001543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           77 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggaagaa
       61 taagtcagag gtgaaagcta gcagcttaac tgttaaattg cctttgatac tgtttttctt
      121 gagtctagtt ggcgttggcg gaatgtgaca tgtagcggtg aaatgcgtag atatgtcaca
      181 gaacaccgat cgcgaaggca gctaacgaag ctagtactga cactgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001544
VERSION     KHUT01001544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgtgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccatgctt
      121 gagggttgga gaggtagctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagag
      181 gaacactcgt ggcgaaggcg agctactgga caacatctga cgctgaggct cgaaggctag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01001545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001545
VERSION     KHUT01001545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           75 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttga
       61 taagtcagtg gtgaaagccc tacgcttaac gtaggaacgg ccattgatac tgtcagactt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001546             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001546
VERSION     KHUT01001546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttagc
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tggctagact
      121 agagtacagt aggggaaggt ggaattccgg gtgtagtggt gaaatgcgta gatatccgga
      181 ggaacaccag tggcgaaagc ggccttctgg actgtgactg acgctcagac gcgaaagcta
      241 gggtagcaaa cggg
//
LOCUS       KHUT01001547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001547
VERSION     KHUT01001547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttga
       61 caagtctgat gtgaaatcta tgggctcaac ccatagcctg cattagaaac tgtcgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001548
VERSION     KHUT01001548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggtggcttgg
       61 aaagtcttac gtgaaagcct gcggctcaac cgcagaagtg cgtaggaaac ttcctcgcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cactgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001549
VERSION     KHUT01001549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcgga
       61 taagtcttgt gtgaaatctc cgggctcaac tcggagtctg cacgagaaac tatctggctt
      121 gagtgtggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001550
VERSION     KHUT01001550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtcggat gtgaaatctc cgagctcaac tcggaaactg cattggaaac taccctgcta
      121 gagggtcgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001551
VERSION     KHUT01001551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcgcg
       61 taagtcaggt gtgaaatctc agggctcaac cctgaaactg cacttgatac tgcgtggctc
      121 gggtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttaccga cgctgaggca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001552
VERSION     KHUT01001552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtctgat gtgaaatctt gcggctcaac cgtaagcggt cattggaaac tgggggactt
      121 gagtgcagga gaggaaagcg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gctttctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001553
VERSION     KHUT01001553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtccgt
       61 cacgtcgggt gtgaaaaccc agggctcaac cctgggcgtg cactcgatac gggcggacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cactgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001554
VERSION     KHUT01001554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcttct
       61 caggtccggt gtgaaatctt acggctcaac cgtaaaatgg cgctggaaac cgggaggctt
      121 gagtgtgcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg gcataactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001555
VERSION     KHUT01001555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           88 g           48 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc acagctcacc tgtggaactg cttggcatac tggcgatctt
      121 gagccactta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgcgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001556
VERSION     KHUT01001556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           90 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgctg
       61 taagtcgggt gtgaaatttc gaggcttaac ctcgaaactg cattcgatac tgcggtgctt
      121 gaggactgga gaggagactg gaatttatgg tgtagcagtg aaatgcgtag agatcataag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001557
VERSION     KHUT01001557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 caagtcaggg gtgaaatccc ggggctcaac ctcggaattg cctttgatac tgtaagtctc
      121 gagtccggaa gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001558
VERSION     KHUT01001558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           90 g           53 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcttat
       61 caggtctggt gtgaaatctc acggctcaac cgtgaaattg cgctggaaac cgatgagctt
      121 gagtgtgcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg gcacaactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001559
VERSION     KHUT01001559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatttg
       61 taagtagggg gtgaaatccc cgggctcaac ccgggaactg ccttctagac tgcaagtctc
      121 gagatcagga gaggcaagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcttgctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001560
VERSION     KHUT01001560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           87 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaatac taccgtgctt
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001561
VERSION     KHUT01001561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 taagtccgtt gtgaaagtcg agggctcaac cctcgaatgc cggcggatac tgtcgggcta
      121 gaatccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001562
VERSION     KHUT01001562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgca ggtggttctt
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tggggaactt
      121 gagtatagaa gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actttctggt ctataactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001563
VERSION     KHUT01001563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 tcagtcggtt gtgaaagtca ggggctcaac ccttgaatgc cgaccgatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001564
VERSION     KHUT01001564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           78 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggtctta
       61 taagtcagtg gtgaaagcct acagctcaac tgtagaactg ccattgatac tgtaagactt
      121 gaattcgatc gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat agcgaaggca gctcactagg tctggattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001565
VERSION     KHUT01001565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggacgag
       61 caagtggtgt atgaaagctc caggcttaac ctggagaggt taggccagac tgctcgacta
      121 gaggcagaga gagggacatg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaactccaga ggcgaaggcg gtgtcctggc tctgacctga cgctcagaca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001566
VERSION     KHUT01001566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          109 g           35 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 gaagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tctcgggctt
      121 gagcactgta ggggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001567             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001567
VERSION     KHUT01001567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           99 g           47 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatca ttgggcgtaa agggctcgta ggcggactag
       61 taagtctggc gtgaaatctc caccgctcac cggggagagg tcgctggata ctgctagtct
      121 tgagttcggt agagggtcgt ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg tggcgaaggc ggcgacctgg gcctgaactg acgctgagga gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001568
VERSION     KHUT01001568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           87 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtccgtg gtgaaagcct ccagctcaac tggagaactg ccatggatac tggcgggctt
      121 gagtacagac gaggttggcg gaattgaaga tgtagcggtg aaatgcatag atatcttcaa
      181 gaactccgat tgcgtaggca gctaactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001569
VERSION     KHUT01001569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctatt
       61 caagtcgggg gtgaaagccc ggggctcaac cccggaactg ccctcgaaac taggtagcta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001570
VERSION     KHUT01001570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           48 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctatt
       61 cgcgtcggat gtgaaagctc ggggctcaac cctgagaggc cattcgatac gggatagcta
      121 gaggcaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttgcctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01001571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001571
VERSION     KHUT01001571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggccggt
       61 caagtgaggc gtgaaagtcc tgggctcaac ctggggacgg cgcttcagac tgtcgggcta
      121 gagtccggaa gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggt ccggtactga cgctcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001572             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001572
VERSION     KHUT01001572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           99 g           47 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggatctg
       61 aaagtctgat gtgaaatctc caccgctcac cggggagggg ccattggata ctgcagatct
      121 ggagttcggt agagggtcgt ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg tggcgaaggc ggcgacctgg gcctgaactg acgctgagga gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001573
VERSION     KHUT01001573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          101 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggctggt
       61 cgcgtctgcc gtgaaagccc gcagcttaac tgcgggtctg cggtggatac gggccggcta
      121 gaggtaggta ggggcaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttgctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001574
VERSION     KHUT01001574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           52 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgcc
       61 taagtacctt gtgaaatccc acagcttaac tgtggaattg cttggtatac tgggtgtctt
      121 gagccactca ggggcgagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001575
VERSION     KHUT01001575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtctgat gtgaaatccc ggggctcaac cccggaactg cattggatac tgccttgctt
      121 gagtactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaaggccagt ggcgaaggcg agttcctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001576
VERSION     KHUT01001576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           44 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtccgc
       61 taagtcatgt gtgaaatccc ccggctcaac cggggaattg cacctgatac tggcgggctc
      121 gaggatggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccataactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001577
VERSION     KHUT01001577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggtttgt
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg catttgaaac tggcaagctt
      121 gagtctcgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccggt ggcgaaggcg gccccctgga cgaagactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001578
VERSION     KHUT01001578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           55 c          107 g           41 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agggtgcgta ggcggctgcg
       61 cgcgtggagg gtgaaatctc tcggcttacc cgggaggggg ctttccagac ggcgtggctt
      121 gagggacgga gaggggcgtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgac ggcgtaggca gcgccctggc cgtttcctga cgctgaagca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001579
VERSION     KHUT01001579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 gacgaactgt gcaaacgtta ttcggaatca ctgggcttga agagtgcgta ggcggtttcg
       61 taagtagggt gtgaaagccc ccggctcaac cggggaattg cgccctaaac tgcgaggctg
      121 gagtgaggta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactgga ccttgtctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001580
VERSION     KHUT01001580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcgcac
       61 taagtcacac gtgaaatccc tcagcttaac tggggaactg cgtgtgaaac tggagcgctt
      121 gaggacagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgtttctga cactgatgaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001581
VERSION     KHUT01001581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgg
       61 caagtgacgg gtgaaatccc tcggcttaac cgaggaactg cctgtcatac tgctaggctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001582
VERSION     KHUT01001582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtaggtg gcaaacgtta tccggaatca ctgggcgtaa agggtccgca ggcggatagt
       61 caagtcggac gtgaaagctc gcggctcaac tgcgagaagc cgttcgaaac tggctatctt
      121 gaatccggca gaggtcgatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaag agcgaaggca gtcggctggg ccgagattga cgctgaggga cgaaggcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001583
VERSION     KHUT01001583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtctgc
       61 caagttcgga gtgacagttt ccggcttaac cggaaaaggt cttcgaaaac tggtggactt
      121 gagaacttga gggggagacg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgaa agcgaaggca gtctcctggc aagtttctga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01001584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001584
VERSION     KHUT01001584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcgggtctt
       61 taagtcagag gtgaaatcct ggagctcaac tccagaactg cctttgatac tgagggtctt
      121 gagtatgaca gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggg tcattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001585
VERSION     KHUT01001585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctggttgc
       61 caagtctgtg gtgaaatcct ccggcttaac cggagaactg ccgtggatac tggcgacctc
      121 gagtggggta ggggcttgcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg gcgagctgga ccctaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001586
VERSION     KHUT01001586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgac gtgaaatctc cgagcctaac tcggaaactg cgtcggatac tattcggctt
      121 gaggattgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001587             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001587
VERSION     KHUT01001587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           91 a           29 c           67 g           65 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agggcacgta ggtggtttgt
       61 tatgtgtact ataaaattgt aaagcataac tttataacgt agtacagaca actaaactag
      121 agttaagtag agagtagtag aatttctaat gtaaaggtga aatttgataa aattagagag
      181 aataccaatg gcgaaggcaa ctacttgggc ttcaactgac actgaggtgc gaaggtatgg
      241 ggagcaaata gg
//
LOCUS       KHUT01001588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001588
VERSION     KHUT01001588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatatt
       61 taagtcgggg gtgaaatccc ggggctcaac cccggaactg ccttcgatac tgggtatctt
      121 gagttcggaa gaggtgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gctcactggt ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001589
VERSION     KHUT01001589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           89 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggctactgag
       61 caagtcagtt gtgaaagccc cgagcttaac ttgggaagtg caactgatac tgcacagatt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001590
VERSION     KHUT01001590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctgtt
       61 caagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tggacggctg
      121 gagaccggaa gaggtcagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctgactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001591
VERSION     KHUT01001591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacgtaggga ccgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgt
       61 caagtcaatt gtgaaatctc tgagcttaac tcagaactgc caattgatac tgatgtgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001592
VERSION     KHUT01001592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtcaggg gtgaaagccc agagctcaac tctggaactg cctttgaaac tagaggactt
      121 gggtacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgttaccga cgctcaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001593
VERSION     KHUT01001593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           89 g           45 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattca ctgggcgtaa agcgcgtgta ggcggccgga
       61 taagttggct gtgaaaactc ctggctcaac taggagaggc cggtcaagac tattcggctc
      121 gaggacggta gaggaaggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaatacctgt ggcgaaagcg gccttctggg ccggacctga cgctcaaacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001594
VERSION     KHUT01001594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c          100 g           39 t
ORIGIN      
        1 gacggaaggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggctgtg
       61 caagtctggc gtgaaagccc cgggctcagc ccgggaagtg cgctcgaaac tgcgcggctc
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001595
VERSION     KHUT01001595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           81 g           56 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggcggatagg
       61 taagtcagag gtgaaactcc gaagctcaac ttcggaactg cctttgatac tgcttatctt
      121 gagtttggga gagggtgatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gtcacctggt ccaatactga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001596
VERSION     KHUT01001596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcggtttac
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tggtagacta
      121 gagagcagga ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctaga ctgtatctga cgctcagaca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001597
VERSION     KHUT01001597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c          100 g           41 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgggca ggtggtggct
       61 taagtcatgc gtgaaagctc ccggctcaac tgggagaggt cgtgtgaaac tgggtcactg
      121 gaggcaggga gaggcagacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gtctgctggc cctgacctga cactcagccg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001598
VERSION     KHUT01001598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           78 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcaggg gtgaaaactt gcggctcaac cgtaagcttg cctttgatac tgaggagctt
      121 gaatacactt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaaa gtgtgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001599             260 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001599
VERSION     KHUT01001599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>260
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c          105 g           38 t
ORIGIN      
        1 gacgggggat gcgagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggcccgg
       61 tcagtccgct gtcaaagcct ggggctcaac cctggagagg cggcggagac caccgggctt
      121 gagtccggta ggggcagagg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccaag ggcgaaggca ctctgctagg cctgatttcg cgactgacac tcagggacga
      241 gagccagggg agcgaatggg
//
LOCUS       KHUT01001600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001600
VERSION     KHUT01001600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          100 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcaagg
       61 tatgtcgtgc gtgaaatctc ccggcttaac tgggaggggc cgtgcgaaac tgcctggctt
      121 gaggtcggaa ggggaaagtg gaattccggg tgtagtggtg gaatacgtag atatccggag
      181 gaacaccagc ggcgaaggcg gctttctggt ccgattctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01001601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001601
VERSION     KHUT01001601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgccccg
       61 caagtcaggt gtgaaagccc tgggctcaac ccaggaagtg cacttgaaac ttcggggctc
      121 gagttctgga gaggatggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctatctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001602
VERSION     KHUT01001602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           79 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctga
       61 caagtcagag gtgaaagacg gtagcttaac tatcgcagtg cctttgaaac tgtttggctt
      121 gaatgtactt gaggtgggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaactccaat tgcgaaggca gctcactaag gtatgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001603
VERSION     KHUT01001603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c          104 g           42 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttagg
       61 taagacctgt gggaaagacc tgggcttaac ccaggaaggt cgcaggtgac tgcctagcta
      121 gagggcggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgttcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001604
VERSION     KHUT01001604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           75 g           61 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggcctta
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaattg ccattgatac tgtagggctt
      121 gagtatagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaaa ctataactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01001605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001605
VERSION     KHUT01001605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agagcacgta ggcgggatgg
       61 tatgtcggtg gtgaaatccc ccagctcaac tggggaactg cctccgaaac tgctttcctt
      121 gaggccgaga ggggtgagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actcactggc tcggttctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001606
VERSION     KHUT01001606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           90 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgattcag
       61 taagtcttat gtgaaagccc agagcttaac tctggaagtg cacgagatac tgctgaaatt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001607
VERSION     KHUT01001607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctttt
       61 taagtcagag gtgaaagccc acgactcaat cgtggaactg cctttgagac tggatggctt
      121 gaagatggga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctaactgga ccattcttga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001608
VERSION     KHUT01001608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttgat
       61 taagtcagtt gtgaaatacg gcagcttaac tgtcgaggtg caattgatac tggttgactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaga ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001609
VERSION     KHUT01001609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtttgg
       61 gaagttggat gtgaaagctc ccggctcaac tgggagaggt cgttcaagac tctcagactc
      121 gaggatggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccatttctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001610
VERSION     KHUT01001610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagccagat gtgaaatccc cgggcttaac ctgggaatgg catttgggac tgcaaggctt
      121 gagtatggca gagggggcta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca ggcccctggg ttaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001611
VERSION     KHUT01001611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c          101 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccatc
       61 tttgtcaggc gtgaaagccc cgggctcaac ctgggaactg cgtctgggac ggggtggcta
      121 gagatcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001612
VERSION     KHUT01001612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtcagag gtgaaatccc agtgctcaac actggaactg cctttgagac tgcatcgctc
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001613
VERSION     KHUT01001613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           94 g           42 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agcgcatgca ggcggttgtc
       61 aaagtcggac gtgaaagctc ctggctcaac taggagaggt cgttcgagac tcggcagcta
      121 gagggtgata gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctggg tcacacctga cgctaagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01001614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001614
VERSION     KHUT01001614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcga
       61 acagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgatac tgccgagctt
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001615
VERSION     KHUT01001615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          104 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcag
       61 taaggttgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtggcaac tgctgagctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001616
VERSION     KHUT01001616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           88 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggtatca ctgggcttaa agcgtgcgta ggcggcctgg
       61 taggtgagat gtgaaagccc acggctcaac cgtggaattg cgtttcaaac caccaggctt
      121 gaggatggca ggggtgatgg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg catcactggg ccatttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001617             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001617
VERSION     KHUT01001617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcggcc gtgaaaaccc agggctcaac cctgggatgc cggtcgatac tgctgtggct
      121 agggtccggt agaggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcgctctgg gccggcaccg acgctgagga gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001618
VERSION     KHUT01001618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatca ttgggcgtaa agagcatgta ggtggatcaa
       61 ttagtctgct gtgaaagtca aaggctcaac ctttgaaagc cggtggatac tgttggtcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001619
VERSION     KHUT01001619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c          100 g           35 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggactg
       61 caagtcggga gtgaaactcc ggggctcaac tccggaccgg ctaccgaaac tgcagacctg
      121 gggaccggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacacccgt ggcgaaggcg gccagctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001620
VERSION     KHUT01001620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           88 g           53 t
ORIGIN      
        1 tacgtaccgt acgaacgtta ttcggaatta ctgggcttaa agggtttgta ggcggccacg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaacgg cgcttgatac tgcgtggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001621
VERSION     KHUT01001621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtctgt
       61 taagtctgat gtgaaagccc cgggctcaac ccgggaagtg cattggatac tgccagactt
      121 gagtacggga gagggtagtg gaatttcgag tgtaggggtg aaatccgtag atattcgaag
      181 gaacaccggt ggcgaaggcg gctacctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001622
VERSION     KHUT01001622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          104 g           38 t
ORIGIN      
        1 gacggaaggt gcgagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggcgcgg
       61 tcagtctggt gtgaaagccc cgggctcagc ccgggaaggg cgctagaaac tgccgcgctg
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001623
VERSION     KHUT01001623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           88 g           52 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtatgta ggcggcgctg
       61 ttagtcgggt gtgaaagccc tcggctcaac cgaggaattg cgcccgatac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgagata cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01001624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001624
VERSION     KHUT01001624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcctga
       61 aaggtcagct gtgaaaactc gaggctcaac ctcgagacgt cggttgaaac catctggcta
      121 gagtccggaa gaggagtgtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gcactctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001625
VERSION     KHUT01001625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          109 g           36 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtccgg
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgcgggactg
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001626
VERSION     KHUT01001626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           84 g           52 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc acagcttaac tgtggaactg cttggtatac tggttgtctt
      121 gaggtaccta ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaac ggtgaaaaca gctcgctggg ggtatcctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001627
VERSION     KHUT01001627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatatc
       61 taagttgggg gtgaaatccc ggggctcaac ctcggaattg ccttcaaaac tggatatctt
      121 gagttcggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001628
VERSION     KHUT01001628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 taagtcactt gtgaaatctc cgggcttaac tcggagcggc caagtgatac tgttgtgcta
      121 gagtgcagaa agggcaatcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001629             251 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001629
VERSION     KHUT01001629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>251
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           94 g           44 t
ORIGIN      
        1 gacggggggg gcaagtgttc ttcggaatga ctgggcgtca agggcacgta ggcggtgaat
       61 cgggttgaaa gtgaaagtcg ccaaaaagtg gcggaatgct ctcgaaacca attcacttga
      121 gtgggacaga ggagagtgga atttcgtgtg taggggtgaa atccgtagat ctacgaagga
      181 aggccaaaag cgaaggcagc tctctgggtc cctaccgacg ctggggtgcg aaagcatggg
      241 gagcgaacag g
//
LOCUS       KHUT01001630             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001630
VERSION     KHUT01001630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           49 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagcgtgaa gtgaaatctc cagggctcaa cccggaaact gctttgcgaa ctggcagact
      121 agaggaaggt agaggtaagt ggaattcccg gtgtagcggt gacatgcatt gataccggga
      181 ggaacaccca tggcgaaggc agcttactgg accttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001631
VERSION     KHUT01001631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           83 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatggt
       61 taagtaggaa gtgaaaggtt ggagctcaac tccgacactg cttcctatac tggctatctt
      121 gagtggcgga gaggaagacg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca gtcttctgga cgcaaactga cgctgagata cgaaagccaa
      241 gggagcaaac agg
//
LOCUS       KHUT01001632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001632
VERSION     KHUT01001632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatttg
       61 ccagtcaggg gtgaaatgcc agggcttaac cttggaactg cctttgatac tgcaagtctg
      121 gagtacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001633
VERSION     KHUT01001633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggttcgg
       61 taagtgaggt gtgaaagccc tcggctcaac cgaggaattg cgcttcaaac tgccgggctc
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccaga ggcgaaggcg gctcactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001634
VERSION     KHUT01001634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggcgatctt
      121 gagccaccta ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001635             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001635
VERSION     KHUT01001635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           45 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcaggt gtgaaaactc caggctcaac ctggagacgc cacctgatac tgctgtggct
      121 agagtccggt aggggagcat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001636             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001636
VERSION     KHUT01001636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c          100 g           47 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggcttcg
       61 caggtctgtg gtgaaagcat acagctcaac tgtattgagc catggaaacg gcggagcttg
      121 aggctgggag gggctggtgg aattccctgt gtagcggtga aatgcgtaga gatgggggag
      181 aacactcgtg gcgaaggcgg ccagctggac cagttctgac gctgagacgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001637
VERSION     KHUT01001637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtccgc
       61 taagacaggt gtgaaattcc tgggctcaac ccaggacgtg cgcctgtgac tgacggactt
      121 gagtccggga gaggatggtg gaactcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacccgt ggcgaaggcg gccatctgga ccggtactga cgctaaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001638
VERSION     KHUT01001638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggttttg
       61 taagtcttgt gtgaaatctt caggctcaac ttgaagactg cacgagaaac tgcagggctt
      121 gagtgtggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001639
VERSION     KHUT01001639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           45 c           97 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacct acagcttaac tgtgggcttg ctttcgatac gggcagacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg catgatctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001640             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001640
VERSION     KHUT01001640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           98 g           41 t
ORIGIN      
        1 tacagagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggaacgg
       61 taaagtccgg agtgaaatct cgccgctcaa cggcgaggct gctttggata ctgacgatct
      121 agggaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccgggtccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001641
VERSION     KHUT01001641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           97 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcggccgtg
       61 tgcgtcagag gtgaaatccc cgggcttaac ccgggaactg cctctgatac ggcatggctt
      121 gagtccggga gaggggagca gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaataccagt ggcgaaggcg gctccctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001642
VERSION     KHUT01001642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtacctt gtgaaatcct gcggctcaac cgcagaacgg ctgggtatac tggcggtctt
      121 gggccaccta ggggcagatg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccgat ggtgaaaaca gtctgctggg ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001643
VERSION     KHUT01001643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgagg
       61 taagtcggat gtgaaatctc caagcttaac ttggaaatgg cattggaaac taccttgctt
      121 gagggtcgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001644
VERSION     KHUT01001644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           87 g           60 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agcgaacgca ggcggtttgg
       61 taagtcaggg gtgaaagacc acagcttaac tgtggtattg cctttgatac tgcctttctt
      121 gagtacagta gggggaagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ctgtaactga cgctcatgtt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001645
VERSION     KHUT01001645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           58 c          109 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctggt
       61 gaaggcttgt gtgaaagtcc ggggctcaac cccggatcgg cacgggcgac tcgctggctg
      121 gagcccacta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg gtggagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001646
VERSION     KHUT01001646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctgat
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaactg cattcgatac tgatcagcta
      121 gagtatgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggt tcaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001647
VERSION     KHUT01001647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggaccgc
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgacagtctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001648
VERSION     KHUT01001648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           94 g           54 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtctgt
       61 tgcgtcggga gtgaaaactt acagcttaac tgtgagcgtg cttccgatac gggcagactt
      121 gaggtattca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg aatatcctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001649
VERSION     KHUT01001649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtttct
       61 taagtctggt gtgaaagccc gcagctcaac tgcggagggc cactggaaac tgggagactt
      121 gagtgtagga gaggagagcg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacacccgt ggcgaaagcg gctctctggc ctataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001650             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001650
VERSION     KHUT01001650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           95 g           42 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagttgtgc gtgaaagctc ccaggctcaa ccgggagagg ccgtgcaaga ctgcccgact
      121 agaggtagag agaggccagt agaattccgg gtgtagtggt gaaatgcgta gagatccgga
      181 ggaataccag aagcgaaggc ggctggctgg ctctaaactg acgctcagac gcgaaagcat
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001651
VERSION     KHUT01001651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           88 g           45 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac tgtcgcgctt
      121 gagtaggcta gaggtaagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gcttactggg acctaactga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001652
VERSION     KHUT01001652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           86 g           51 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggattta ttgggcttaa agggtatgta ggcggccctt
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaactg cgcccgatac tgtggggctc
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgagata cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001653
VERSION     KHUT01001653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           96 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtcttt
       61 taagtccgtt gtgaaagccc tgggctcaac ctgggaatgg cagtggatac tggaaggctg
      121 gagtacggta gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccctctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001654
VERSION     KHUT01001654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacatt
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tgggtgtctt
      121 gagtatggaa gaggttagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gctaactggt ccataactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001655
VERSION     KHUT01001655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           97 g           42 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgta ggcggttgca
       61 tgcgtccgcg ctgaaatccc aaggctcaac cttggggcgc gtgtggatac gatgcgactt
      121 gaggctcgga gagggtggga gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaatacccgt ggcgcaagcg cccacctggc cgagacctga cgctaaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01001656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001656
VERSION     KHUT01001656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           44 c           95 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggtgtaa agggtgcgta ggctggcgcc
       61 taagtctggg gtgaaatctt gccgcttaac ggtaaaatgg ccttggatac tgggtgtctt
      121 gaatctgaca gaggtcggtg gaattcgtgg tgtagcggtg aaatgcatag atatcacgaa
      181 gaacatcagt ggcgaaggcg gccggctggg tcagtattga cgctgaggca cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001657
VERSION     KHUT01001657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          101 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtgccg
       61 taagtctggt gtgaaatccc cgagcttaac tcgggtcccg cgccagagac tgcggtgctg
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccactactga cgctgaggcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001658
VERSION     KHUT01001658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          103 g           40 t
ORIGIN      
        1 tacggaaggg gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggccttg
       61 caagtgcggc gtgaaagtcc tgggctcaac ctggggacgg cgctgcggac tgcacggctg
      121 gagtccggaa gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggt ccggcactga cgctggagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001659
VERSION     KHUT01001659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           95 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttggc
       61 gaagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaaaac tggctgacta
      121 gagcatggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccatgactga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001660
VERSION     KHUT01001660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           81 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcagtg tttaaagtca acagcttaac tgttggaatg gcaatgatac tgcaaggctt
      121 gagtaaagtt gaggtgggcg gaattgatgg tgtagcggtg aaatgcatag atatcatcaa
      181 gaacaccgat tgcgaaggca gctcactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001661             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001661
VERSION     KHUT01001661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcggat gtgaaatctc cacgctcaac gtggaggggc cattcgagac tgctatgact
      121 agagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001662             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001662
VERSION     KHUT01001662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           87 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgta ggtggtcatg
       61 caagtctatt gtcaaagcac aaggcttaac cttgtaaagg cagtggaaac tgcattaact
      121 tgagtgcagt aggggtagag ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 agaacaccag tggcgaaagc gctctgctgg actgcaactg acactgaggg acgaaagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01001663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001663
VERSION     KHUT01001663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtccga
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgccggactt
      121 gagtacagga gaggatcgcg gaattctcgg tgaagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001664             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001664
VERSION     KHUT01001664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           50 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttt
       61 taagtcttgt gtgaaatccc tcggctcaac tgaggaactg cacgggatac tggaagactt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgattactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001665
VERSION     KHUT01001665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           87 g           47 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcag
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaattg cgcccaaaac tactgtgctc
      121 gagggaggta gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga cctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001666
VERSION     KHUT01001666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           81 g           63 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaatcca acagctcaac tgttgatgtg ccattgatac tggtagcctt
      121 gagtacagat gaggtaggcg gaattgacag tgtagcggtg aaatgcatag atattgtcaa
      181 gaactccgat agcgaaggca gcttactggg ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001667
VERSION     KHUT01001667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgt
       61 taagtcggct gtgaaagccc ggggctcaac cccggagggt caaccgatac tggcaggcta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001668
VERSION     KHUT01001668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           77 g           60 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaagat
       61 taagtcagta gtgaaagcct cccgctcaac gggagaactg ctattgatac tgattttctt
      121 gaatatagtt gaagtaggcg gaatacgaca tgtagcggtg aaatgcatag atatgtcgtg
      181 gaacaccgat cgcgaaggca gcttactaag ctatcattga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001669
VERSION     KHUT01001669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           92 g           45 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgtgg
       61 taagtcagat gtgaaatgcc ggggctcaac cccggaacgg catccgatac tgccatgcta
      121 gagtagtgga ggggcgactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg agtcgctgga cacttactga cgctgaggca cgaaggccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01001670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001670
VERSION     KHUT01001670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          101 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcgggcggg
       61 caagtctctc gtgaaatccc acggctcaac cgtggaactg cgggagatac tgtctgtctt
      121 gagtccggga gaggagggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001671
VERSION     KHUT01001671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taggtctgtt gtgaaaactg gaggcttaac cttcagaggt cgatggaaac catctggcta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001672
VERSION     KHUT01001672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           76 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggatttg
       61 taagtctgag gtgaaatctc cgagcttaac tcggaaactg ccttggatac tataagtctt
      121 gaatgtcgtt gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gctgactaag cgaatattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001673
VERSION     KHUT01001673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggacagt
       61 taagttgggg gtgaaagccc ggggctcaac ctcggaattg ccttcaatac tggctgtctt
      121 gagtgtgaga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc tcactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001674
VERSION     KHUT01001674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           97 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggtctgt
       61 ttagtctagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcagactc
      121 gagggatgca gaggaaagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggg cattacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01001675             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001675
VERSION     KHUT01001675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           95 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggacgg
       61 cacgtctgtc gctgaaatcc cccggcttaa ccggggaact ggcgtagata cgaccgtcct
      121 tgaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01001676             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001676
VERSION     KHUT01001676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           47 t
ORIGIN      
        1 tacgtagggt gctaacgttg ttcggaatca ctgggcataa agagcgcgta ggcggtctga
       61 taagtcaggt gtgaaatccc ccggctcaac cggggaactg cacctgatac tgttgggctt
      121 gagtgcagga ggggagagcg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggc ggcgaaagcg gctctctggc ctgtctactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001677
VERSION     KHUT01001677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctttg
       61 caagtgacgg gtgaaatccc acggcttaac cgtggaactg cctgtcagac tgcatggctt
      121 gagaccagga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001678
VERSION     KHUT01001678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccgttgatac tatctgtctt
      121 gaatatcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctaactact cgatcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001679
VERSION     KHUT01001679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           46 t
ORIGIN      
        1 cacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctgtc
       61 cacgtcggat gtgaaagctc ggagctcaac tctgagaggc cattcgatac gggacagcta
      121 gaggcagcta ggggagagtg gaatacctgg tgtagcggtg aaatgcgcag atatcaggtg
      181 gaacaccggt ggcgaaggcg gctctctggg gctgttctga cgctgaggcg cgaaagctgg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001680
VERSION     KHUT01001680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tggcggtctt
      121 gagccaccta ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaac ggtgaaaaca ggttgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001681
VERSION     KHUT01001681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcatac gtgaaatccc tcggctcaac cgaggaactg cgtgtgatac tggcgggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgagattga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001682
VERSION     KHUT01001682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           88 g           46 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg ctgggtatac tggcgatctt
      121 gggccattca ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg aatgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001683
VERSION     KHUT01001683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcctt
       61 taagtcagtg gtgaaatctc cgggcctaac ccggaaactg ccattgatac tattggtctt
      121 gaattcagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001684             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001684
VERSION     KHUT01001684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cacgtctgcc gtgaaaaccc gaggcttaac ctcgtggctt gcggtggata cgggcagact
      121 agaggtaggt aggggagaac ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccgg tggcgaaggc ggttctctgg gccttacctg acgctgagga gcgaaagcat
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001685             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001685
VERSION     KHUT01001685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcatt
       61 taagtctggt gtataaacca agggctcaac ctttggacgc attggaaact gggtgactgg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001686
VERSION     KHUT01001686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           60 c          108 g           37 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggtggctcac
       61 tgcgcccggc gtgaaagccc ccggcctaac cggggagggt cgtcggggac gggtgagctt
      121 gagggtagca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg ctacacctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001687
VERSION     KHUT01001687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 caagtcaggc gtgaaagccc cgggctcaac ccgggaattg cgcttgatac tgtcgtgctt
      121 gaggccggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001688
VERSION     KHUT01001688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           85 g           50 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaattg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgcttttctt
      121 gagccactta ggggctgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca gacagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001689
VERSION     KHUT01001689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtctgat gtgaaatctc cgggctcaac ccggaaatgg cattggatac tattcggcta
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001690
VERSION     KHUT01001690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggtggttcag
       61 tgtgtcggtc gtgaaatccc cgggcttaac ctgggaactg cggccgaaac tgctgagcta
      121 gagtgtggta gagggaggtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgaaggca gcctcctggg ccacaactga cactgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001691
VERSION     KHUT01001691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tggcaatctt
      121 gaggctggaa gaggttggta gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaataccagt ggcgaaggcg gccaactggt ccagatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001692
VERSION     KHUT01001692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           98 g           47 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcttta
       61 caagtatggc gtgaaatctc cctgctcaac ggggagggtg cgttgtagac tgtaaggctt
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgacc cgg
//
LOCUS       KHUT01001693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001693
VERSION     KHUT01001693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgcc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tgggtgtctt
      121 gagccactca ggggctaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtagctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001694
VERSION     KHUT01001694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcgggctga
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tgtcagtctc
      121 gagtccggaa gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcccactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001695
VERSION     KHUT01001695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgtaagtg gcgaacgatg tccggattca ctgggtgtaa agcgcatgta ggcggcttgg
       61 taagtctgag gtgaaatcca cgggcctaac ccgtgaactg ccttggaaac tgccgagctc
      121 gagggccaga ggggaaagtg gaatttcggg tgtagcggtg aaatgcgtag atatccgaga
      181 gaacaccgat ggcgaaggca gctttctggc tggttcctga cgctgagatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001696
VERSION     KHUT01001696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          100 g           48 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggcggtc
       61 catgtctggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac ggggctgctg
      121 gagtgcggga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagacg gcttactgga ccgtgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001697
VERSION     KHUT01001697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           94 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtccggg gtgaaatccc acagctcaac tgtggaactg cctcggatac tgggggtctc
      121 gagggtggca ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactggt ccacttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001698
VERSION     KHUT01001698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcttgt
       61 caagtagggt gtgaaagccc ccggctcaac cggggaattg cgccctaaac tggcgagctg
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactgga tcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001699
VERSION     KHUT01001699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggctgcgggg
       61 caagtcgggt gtgaaatctc agagctcaac tctgaaactg cactcgatac tgctttgctt
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggcg cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001700             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001700
VERSION     KHUT01001700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           96 g           44 t
ORIGIN      
        1 cacgtaggga ccaagcgttg tccggattta ttgggcgtaa agcgctcgta ggcggttcgg
       61 taagtcgggt gtgaaacccc tgggctcaac ccggggacgc cacccgatac tgctgtgact
      121 cgagttcggt aggggagcgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccgctctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001701
VERSION     KHUT01001701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           76 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaattg
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaattg ccattgatac tgcaaatctt
      121 gagtacattt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001702
VERSION     KHUT01001702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           87 g           53 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttat
       61 taagtctgct gttaaagcac agggctcaac cctgtagagg cggtagaaac tggtaggctt
      121 gagtatggta ggggtagagg gaattcccga tgtagtggtg aaatgcgttg atatcgggaa
      181 gaacaccaaa ggcgaaagca ctctgctggg ccaatactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01001703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001703
VERSION     KHUT01001703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          104 g           41 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcccat
       61 caagtcgagt gtgaaagccc cgggctcaac ctgggaggtg cgctcgagac tggcgggctg
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001704
VERSION     KHUT01001704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggtaagg
       61 taagtcggat gtgaaagctc cgggctcaac ccggaaatgg cattggaaac tactttgctt
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001705
VERSION     KHUT01001705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttgtt
       61 taagtctgtt gtgaaagccc tgggctcaac ctgggaactg cagtggaaac tggacaacta
      121 gagtgtggta gagggtagcg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatccat ggcgaaggca gctacctgga ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001706
VERSION     KHUT01001706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcggtg
       61 taagttggac gtgaaagccc ttggcttaac caaggaagtg catccaatac tgcgtcgctt
      121 gagtgtcgga gggggcggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccgcctgga cgataactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001707
VERSION     KHUT01001707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtctgt
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaattg catttgatac tggcaggctc
      121 gagaacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtcactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001708
VERSION     KHUT01001708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttcgt
       61 taagtcagat gtgaaagccc tcggctcaac cgaggaagtg catctgaaac tggcgagcta
      121 gagtactaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001709
VERSION     KHUT01001709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           80 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggcctat
       61 taagtcaggg gtgaaagacg gtagctcaac tatcgcagtg cctttgatac tgatgggctt
      121 gaatacagtt gaagtgggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gctcactaaa gtgtgattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001710             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001710
VERSION     KHUT01001710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          102 g           44 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 gaagtcggat ttgtgaaagc ccggggctca actccggagg tgcatccgag actctcaggc
      121 tggaggccgg aagaggaggg tggaataccc agtgtagagg tgaaattcgt agatattggg
      181 tggaacaccg gtggcgaaag cggctctctg gtccggtcct gacgctgagg cgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01001711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001711
VERSION     KHUT01001711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           93 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggccctt
       61 caagtctaat gtgaaagccc gaggcttaac ctcggaaggg cattagaaac tgagaggctc
      121 gagtaccgga agggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg actctctgga cggttactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001712
VERSION     KHUT01001712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           87 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctgtt
       61 caagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgaaac tagacagcta
      121 gaatcatgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001713
VERSION     KHUT01001713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           94 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcccgca ggcggcccgc
       61 caagtttggg gtgaaagctc ctcgcttaac gaggagagag ccgcaaaaac tggtggactt
      121 gagagcgaga gagggacacg gaactccggg tgaagcagtg aaatgcgtag atatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtatctga cgctcagggg cgaaagccag
      241 ggaagcgaac ggg
//
LOCUS       KHUT01001714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001714
VERSION     KHUT01001714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgccc
       61 taagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tggggtgctc
      121 gagtgtggga gaggcaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgc ggtgtagacg gcctgctgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001715
VERSION     KHUT01001715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           95 g           41 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcgat
       61 caagtacctt gtgaaatccc acggctcaac cgtggaaccg ctgggtatac tggttgtctt
      121 gagccggcta ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccgaa ggtgaaaaca gctcgctggg gccgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001716
VERSION     KHUT01001716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcgaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggcggca
       61 taagcggaat gtgaaagccc agggctcaac cctggaagtg catcccgaac tgtgtcgctt
      121 gaatctcgga gggggacaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgtcctgga cgaagattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001717
VERSION     KHUT01001717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          103 g           43 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggtccg
       61 caagtcgatc gtgaaatcct tcggctcaac tgaagggggt cggtcgatac tgcgggtctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg catctcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001718
VERSION     KHUT01001718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacgtggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccttt
       61 taagtcggat gtgaaagccc caggcttaac ctgggaagtg catccgatac tggaaggctt
      121 gagtactaga gggggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga tagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001719
VERSION     KHUT01001719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 tacgtaggga cctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgtt gtaaaagtcg aaggctcaac cttcgaaagc cgatggatac tgccgggcta
      121 gagtacggaa gaggcgagta gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaatacccat agcgaaggca gctcgctggg acgttactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001720
VERSION     KHUT01001720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           75 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggacctg
       61 taagtcagtg gtgaaatctc cccgctcaac ggggaaactg ccattgatac tgcaggtctt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccgat tgcgaaggca ggttactacc aatacattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001721
VERSION     KHUT01001721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggctgaa
       61 taagtggggt gttaaatccc cgggctcaac ccgggaactg cacttcatac tgttcagctt
      121 gagtacatca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg atgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001722
VERSION     KHUT01001722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccccg
       61 caagtcactt gtgaaatctc cgggcttaac tcggaacggc caagtgaaac tgcggagcta
      121 gagtgtggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccaacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001723
VERSION     KHUT01001723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggactct
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tgagagcctt
      121 gagtccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001724
VERSION     KHUT01001724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcatac
       61 caagtattca gtgaaatctc cctgctcaac ggggagggtg ctgagtagac tggtaagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtttctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001725
VERSION     KHUT01001725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatatt
       61 taagttagag gtgaaagccc agggctcaac cctggaatag cctttaatac tggatatctt
      121 gagttcggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001726
VERSION     KHUT01001726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          102 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggccgat
       61 taagtcaggt gtgaaaggct gcggctcaac cgcagagttg catctgaaac tggtcggctt
      121 gagtgcagga gaggggagcg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001727             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001727
VERSION     KHUT01001727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          104 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgcag
       61 cgtgtcggct tgtgaaagcc cggggctcaa ccccggaggt gcggccgaaa ctgctgtgct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggacctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001728
VERSION     KHUT01001728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggttcgt
       61 aaagtcagat gtgaaagccc tgggcttaac ccaggaagtg catttgaaac tcacgaactt
      121 gagtcccgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacatcggt ggcgaaggcg gccttctgga cggtgactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001729
VERSION     KHUT01001729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           44 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaatccc ggggctcaac cccggaactg cattcgatac tgcctcgcta
      121 gagtcccgga gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccctctgga cggtgactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001730
VERSION     KHUT01001730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccgg
       61 catgtcgggt gtgaaatccc tcggcttaac cgaggaagtg cattcgaaac tatcggactt
      121 gagtactgaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001731
VERSION     KHUT01001731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacgagaggc ccaaacatta ttcggaatta ctgggcttaa agagttcgta ggcggcgtgg
       61 taagtagggt gtgaaagccc tcggctcaac cgaggaattg cgctctaaac taccgtgcta
      121 gaggaagcta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001732
VERSION     KHUT01001732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           98 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 atagtctgtg gtgaaagcct ccagcttaac tggagaagtg ccatggatac tgccggcctg
      121 gaggagggta ggggcatctg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001733
VERSION     KHUT01001733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatggc
       61 acagtcagat gtgaaattcc cgggcttaac ctgggggctg catttgatac gtgttgtcta
      121 gagtgaggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001734
VERSION     KHUT01001734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          103 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgagtctg ccatgcgaac tggtggactt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001735
VERSION     KHUT01001735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           83 g           57 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcgtcccgg
       61 caagttttag gtgaaatgct caagctcaac ttgagagctg cctgaaaaac tgccgggatt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactagg ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001736
VERSION     KHUT01001736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           39 c           83 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtttta
       61 taagtcaggg gtgaaagcat gcagcttaac tgtataattg cctttgatac tgtaagactt
      121 gaataaggtt gaggttggcg gaatgtgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctgactggg cctttattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001737
VERSION     KHUT01001737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcttcg
       61 caagtcagac gtgaaatccc cgggcttaac ctgggaatgg cgtttgaaac tacggagctg
      121 gagtgtggca gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001738
VERSION     KHUT01001738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           92 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcagtg
       61 caagtcggat gtgaaatccc gcggctcaac cgtggaagtg cacccgaaac tgcacagcta
      121 gagtgtcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgacgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001739
VERSION     KHUT01001739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           91 g           43 t
ORIGIN      
        1 tacgagtggc ccaagcgtta atcggaatca ctgggcttaa agcgtgcgca ggcggacctg
       61 aaggcgtctt gtgaaatccc acggctcaac cgtggaattg cagggcgaac cacaggtctt
      121 gaggcaggta gggggagatg gaactgtcgg tggagcggtg aaatgcgtag atatcgacag
      181 gaacgccggt ggcgaaggcg atctcctggg cctgtcctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001740
VERSION     KHUT01001740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtttgc
       61 caagtcagag gtgaaagccc cgagctcaac tcgggaactg cctttgaaac tggtagacta
      121 gggttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgataccga cgctcaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001741
VERSION     KHUT01001741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccgaa
       61 taagtctgat gtgaaatccc tcggctcaac cggggaactg catttgaaac tgttcggctc
      121 gagtccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01001742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001742
VERSION     KHUT01001742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          108 g           39 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgtgcgca ggcggcttcc
       61 tgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac ggggaagctt
      121 gagggtatca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg atacacctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001743
VERSION     KHUT01001743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggtggcctgt
       61 taagttccgt gtgaaatctc ccggctcaac cgggagggtg cgcggaaaac tggcgggcta
      121 gagtacggga tgggagagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggtgtagacg gctctctgga ccgtaactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001744
VERSION     KHUT01001744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c          100 g           42 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtac agggagcgta ggtggttagg
       61 taagcctctc gggaaatctc caggctcaac ctggaaaggt cgagagggac tgcctagcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtccctga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001745
VERSION     KHUT01001745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          103 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtccgc
       61 taagtgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcaaac tgtcggactc
      121 gaggactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaagaccggt ggcgaaggcg acctgctggg cagtcactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001746
VERSION     KHUT01001746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacagaggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttaa
       61 caagtcccgt gtgaaatccc tcggctcaac tgaggaactg cacgggaaac tgtttggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001747
VERSION     KHUT01001747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           83 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttg
       61 taagtcagtg gtgaaatacg gtagcttaac tatcgaggtg ccattgatac tgcagagctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001748
VERSION     KHUT01001748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           44 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcatgg
       61 caagtaaggt gtgaaatcca tctgctcaac gggtggggtg cactttagac tgccaagcta
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001749
VERSION     KHUT01001749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           99 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggacaca
       61 tcagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgtttgagac ggtgtgtctt
      121 gagtttggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt cctggactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001750
VERSION     KHUT01001750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgtta tccggattta ctgggcgtaa agggtgtgta ggcggttggg
       61 caggtggggt gttccagcgt gcggcttaac tgcacgagtg cgctccaaac cgcttgactc
      121 gaggggtgca gaggttggtg gaattgccag tgtagcagtg aaatgcgtag agattggcag
      181 gaacaccaag ggcgcaagca gccaaccagg caccacctga cgctgaggca cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001751
VERSION     KHUT01001751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          106 g           38 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtaa agggagcgta ggtggtgggg
       61 taagccccgc gggaaagctc caggcttaac ctggaaaggt cgcgggggac tgctccgctc
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acggccctga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001752
VERSION     KHUT01001752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcgatt
       61 caagtggcgg gtgaaatccc tcggcttaac cgaggaactg cctgccagac tggattgctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001753
VERSION     KHUT01001753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttatc
       61 taagtagggt gtgaaatgcc ccggctcaac cggggaactg cgctctagac tggatggctt
      121 gagtgagata gaggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggagg
      181 gaacaccgtt ggcgaaagcg gcacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001754
VERSION     KHUT01001754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          101 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gaagtcatcg gtgaaagttc agggctcaac tctgaatcgg ccggtgatac tctcgtgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001755
VERSION     KHUT01001755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggtggctgcg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cacccgatac tgcgtagctc
      121 gagtaccgga gaggttggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaatacctgt ggcgaaggcg gccaactgga cgagtactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001756
VERSION     KHUT01001756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgtgca ggcgggtatg
       61 caagtctgat gtgaaagccc cgggctcaac ctgggaagtg cattggaaac tgcatatctt
      121 gagtgctgga gaggaagggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaataccagt ggcgaaggcg cccttctgga cggcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001757
VERSION     KHUT01001757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcacgta ggcggactcg
       61 taggtcaatt gtgaaatacc tgggctcaac ccgggaactg cagttgaaac cgcttgtctt
      121 gaatacctga gagggtgggg gaatacctgg tggagaagtg aaattcgtag agatcaggtg
      181 gaacaccgga ggcgaaggcg cctgcctggc agtgtattga cgctgaggtg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01001758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001758
VERSION     KHUT01001758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtggtc
       61 caagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgggccgctg
      121 gagtacggga gaggcaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggtgcagacg gcttgctgga ccgtgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001759
VERSION     KHUT01001759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           87 g           54 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggctgtgcag
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaactg cgccgaatac tgctgtgcta
      121 gaggaagata ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactgga tcttttctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001760
VERSION     KHUT01001760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagacaggt gtgaaatccc cgagctcaac ttgggaatgg cgcttgttac tggcaagcta
      121 gagtgtgtca gagggaggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gcctcctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001761
VERSION     KHUT01001761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttga
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccattgatac tattgatctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctacg cgaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001762             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001762
VERSION     KHUT01001762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgtag
       61 taagtcgaat gtgaaatctc ggggcttaac cccgaaactg cattcgatac tgctatgcta
      121 gaggtttgta gaggagatcg gaatttacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaagcg gatctctggg caaagtcctg acgctgaggc acgaaggcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001763
VERSION     KHUT01001763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           97 g           37 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggccgc
       61 caagttcgag gtgaaatcct ccggctcaac cggagaactg cctcgaatac tggaggcctc
      121 gaggcgggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga cccgatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001764
VERSION     KHUT01001764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          102 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcggcctgt
       61 taagtcgggt gtgaaaggct gcggctcaac cgcagagcgg catccgaaac tggcgggctt
      121 gagtgcagga gagggaagcg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001765
VERSION     KHUT01001765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           74 g           58 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacatg
       61 caagtcagtg gtgaaatctc tctgctcaac agagaaactg ccattgaaac tatgtgtctt
      121 gagtacattt gaggcgggcg gaatgtgtca tgtagaggtg aaattcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001766             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001766
VERSION     KHUT01001766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           45 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcaact gtgaaagctc cgggctcaac tcggagaggc cagttgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001767
VERSION     KHUT01001767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcaggg
       61 taagttcggt gtgaaagctt cgagctcaac tcggaaacgg cattgaatac tatcctgctt
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ggtctctgga cgattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001768
VERSION     KHUT01001768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccctc
       61 taagtcagac gtgaaatccc tcggctcaac cggggaactg cgtctgagac tggaaggctt
      121 gaattcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgatattga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001769
VERSION     KHUT01001769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           97 g           41 t
ORIGIN      
        1 tacaggggga gcgaacgtta ctcggaatca ctgggcataa agggcgcgta ggcggtcttg
       61 taagtcggat gtgaaagacc ttggctcaac caaggaatgg catccgatac tgcgaggctt
      121 gaggccgaga ggggcaagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttgctgga tcggttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001770             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001770
VERSION     KHUT01001770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggctgag
       61 taagtcggcg gtgaaattcc aaggctcaac cttgaaactg ccgtcgatac tgctctggct
      121 agagtccggt agaggagaac ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacacctg tagcgaaggc ggttctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001771
VERSION     KHUT01001771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           93 g           51 t
ORIGIN      
        1 tacgtaggtg gctagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtcgtt
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cactcgatac tgatcgactt
      121 gaaggcagaa gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggt ctgttcttga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001772
VERSION     KHUT01001772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           93 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttgtt
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaatac tgaatgacta
      121 gagtgtggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctcactgga ccataactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001773
VERSION     KHUT01001773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggctttg
       61 tgagtcggat gtgaaagctc ccggcttaac tgggagaggc cattcgagac tgcaaggctt
      121 gaggttggga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaagcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001774
VERSION     KHUT01001774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           85 g           54 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttgt
       61 taagttgggt gttaaatctc ttggctcaac tgagagatgt cgctcaaaac tagcagactg
      121 gaggacggta gaggaaggca gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccagt ggcgaaggcg gccttctgga ccgttcctga cactcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001775
VERSION     KHUT01001775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatctt
       61 taagtcagag gtgaaatccc aaggctcaac cttggaactg cctttgatac tggggatctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001776
VERSION     KHUT01001776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          100 g           42 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggacgg
       61 caagtcggtt gtgaaatcct ccggctcaac cggagaacgt cgaccgatac tgccgttctt
      121 gagggttgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caactcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001777
VERSION     KHUT01001777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           80 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcata
       61 taagtcagtg gtgaaatact tcagctcaac tggagaggtg ccattgatac tgtatgactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaga ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001778
VERSION     KHUT01001778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtcggat gtgaaaactg ggggctcaac cctcagcctg catccgatac tggcagacta
      121 gagtgcgaga ggggaaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctgga tcgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001779
VERSION     KHUT01001779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           82 g           62 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcaggg gtgaaagacg gcagcttaac tgtcgcagtg cctttgatac tgataggctt
      121 gaatgtggtt gaagatggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaactccgat tgcgaaggca gctgtctaag tcattattga cgctgatgca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01001780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001780
VERSION     KHUT01001780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccattgatac tatcagtctt
      121 gaataccgtg gaggtcggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctgactacg cgagtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001781
VERSION     KHUT01001781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           98 g           41 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttcg
       61 taagtcccgc gtgaaagccc ccggctcaac tggggaatgg cacgggaaac tgcgaggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001782
VERSION     KHUT01001782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           91 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcgtct
       61 taagtctggt ttgaaagcag gcggctcaac cgtctgatgt ggctggaaac tggggcgctt
      121 gaatgcgttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acgtgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001783
VERSION     KHUT01001783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           87 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttag
       61 caagttagag gtgaaagccc gaagctcaac ttcggaattg cctttaaaac tgcaaggcta
      121 gaattgtgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactgga cacatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001784
VERSION     KHUT01001784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           89 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggatttt
       61 taagtgagat gtgaaatacc cgggcttaac ttgggtgctg catttcaaac tggaagtcta
      121 gagtgcagga gaggagaagg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg cttctctgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001785
VERSION     KHUT01001785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggtggttctt
       61 taagtctgat gtgaaatctc gcggctcaac cgcgagcggt cattggaaac tggggaactt
      121 gagtgcagaa gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001786
VERSION     KHUT01001786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           84 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtatgt
       61 taagttcgtc gttaaagact ccggcctaac cggagcaccg cgatgaaaac tggcaaacta
      121 gagggtgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001787
VERSION     KHUT01001787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          104 g           40 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggagcg
       61 tgagtcggtc gtgaaagcct tcggctcaac cgaagaaggg cggccgatac tgcgttcctt
      121 gaggggtgga gaggagactg gaattcccgg tgtagcggtg aaatgtgtag atatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggc cacaccctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001788
VERSION     KHUT01001788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          104 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcat
       61 aaaggctgtg gtgaaagttc ggggctcaac ctcgaatcgg ccgtggcgac ttgtgggctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001789
VERSION     KHUT01001789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtctct
       61 taagtttgga gtgacagttg gtggcttaac tgccaaagtg cttcgaaaac tgagagactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaaa ggcgaaggca gtgtcctggg gagtacctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001790             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001790
VERSION     KHUT01001790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           50 t
ORIGIN      
        1 tacggggggg gcaaacgttg ctcggaatca ctgggcgtaa agcgtgtgta ggcggtccaa
       61 cttgtcgaat gtgaaatacc ttggcttaac caaggaactg cattggaaac ggttggactt
      121 gagtaccgga ggggatgacg gaattcccgg tgtagtggtg aaatacgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gtcatctgga cggatactga cgctgagtac acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001791
VERSION     KHUT01001791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 taagtcggat gtgaaatccc tcggcttaac cgaggacgtg catccgaaac tgcgaggctc
      121 gagtatggaa gagggtcgca gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001792
VERSION     KHUT01001792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          111 g           36 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctct
       61 taagggggcg gtgaaagccc ggggcttaac cccgggtcgg ccgtgccgac tgggaggctg
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001793
VERSION     KHUT01001793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           94 g           42 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctacc
       61 taagtcggat gtgaaagccc ctggctcaac cggggaagtg cacccgaaac tggatagctt
      121 gagtgcaaaa gagggctgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcagcctggg ttgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001794
VERSION     KHUT01001794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           89 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcttgc
       61 caagtcggac gtgaaatccc tcagctcaac tgaggacgtg catccgaaac tggcgagctt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001795
VERSION     KHUT01001795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacataggct ccaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggctgtccgc
       61 caagtcgatt gtgaaatctc ccggctcaac tgggagggtg cggtcgaaac tggcggacta
      121 gagttcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgaagacg gctctctgga ccgatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001796
VERSION     KHUT01001796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          101 g           48 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 cgcgtcgtgc gtgaaaaccc gtagctcaac tgcgggcttg cgttcgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001797
VERSION     KHUT01001797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          101 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctttt
       61 ggtgtcggct gtgaaagccc ggggctcaac cccggaggtg cagccgaaac tcagaggctc
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001798
VERSION     KHUT01001798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggccaag
       61 taagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgctcggctc
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001799
VERSION     KHUT01001799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtccgtg gtgaaatccc tgagcttaac tcaggaactg ccgtggatac tatcactctt
      121 gaatgctgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca cggttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001800
VERSION     KHUT01001800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtatg
       61 taagtcagag gtgaaatccc cgagcttaac ttgggaactg cctttgatac tatatatctt
      121 gaatgtcgta gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactatg cgattattga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001801
VERSION     KHUT01001801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggttttg
       61 taagtctgac ctgaaagtgg gccgcttaac ggcacagggt ggttggatac tgcagaactt
      121 gaagagggtg gaggccgccg gaacggatcg tgtagcggtg aaatgcatag agatgatcca
      181 gaaccccgat tgcgtaggca ggcggctacg ccccacttga cactgaggca cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001802
VERSION     KHUT01001802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           97 g           40 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactta ctgggcgtaa agcgcacgca ggcggtcacg
       61 ggcgtcggtg ctgaaagcgc gccgcttaac ggcgcaatgc gtaccgatac gccgtgactg
      121 gagcaatgca gaggccagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca gctggctggg catttgctga cgctcatgtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01001803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001803
VERSION     KHUT01001803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           97 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtttcg
       61 taagtctggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tgcggagctg
      121 gagtgtggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttgctgga ccactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001804
VERSION     KHUT01001804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           79 g           61 t
ORIGIN      
        1 tacgtagggt gctagagttg tccggattta ctgggtgtaa agggtgctca ggtggatttg
       61 taagtcagag gtgaaatccc aaagcttaac tttggtgctg cctttgatac tgcaagtcta
      121 gagtttgaga gagggcaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttgcctggc tcaaaactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001805
VERSION     KHUT01001805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           90 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagtgta ggtggatgct
       61 taagtcagat gtgaaatacc cgggctcaac ttgggtgctg catttgaaac tgggcgtcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001806
VERSION     KHUT01001806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggcccgc
       61 taagtctgat gtgaaatccc ctcgctcaac gagggaactg cgtcggaaac tggcggactc
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001807
VERSION     KHUT01001807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           87 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tgggtgtctt
      121 gagccatcta ggggcctgcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggtgaaaaca gcaggctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001808
VERSION     KHUT01001808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtgcgg
       61 taagtctctt gtgaaatctc cgggctcaac tcggagcctg caagggaaac tgccgtgctt
      121 gagtatggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccattactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001809
VERSION     KHUT01001809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggtgtcta ggcggtcagg
       61 taagtgagat gtgaaatgcc ccggcttacc cggggacctg catctcatac tgtctggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccaatactga cgctgaagca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001810
VERSION     KHUT01001810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           87 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtattg
       61 caagtcggat gtgaaatccc tcggcttaac caaggaagtg catccgaaac tgcagtactt
      121 gagtacgaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001811
VERSION     KHUT01001811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           87 g           59 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agcgaacgca ggtggtttgg
       61 taagtcagag gtgaaagtcc acagcttaac tgtggcactg cctttgatac tgcctttctt
      121 gagtgcatga gggggaagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcttcctggc atgtaactga cactcatgtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001812
VERSION     KHUT01001812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcagga gtgaaatacg gcagctcaac tgtcgcagtg ctcttgatac tgataggctt
      121 gaatgtcgct gaagatggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gctgtctaaa cgattattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001813
VERSION     KHUT01001813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagtccgct gtgaaagccc cgggctcaac ctgggaatgg cggtggaaac tggcgagcta
      121 gagtgtgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001814
VERSION     KHUT01001814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           96 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgccc
       61 taagtctgtt gtgaaatctc ccggcttaac tgggagggtg cggtggaaac tggggtgctt
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcttactgga ccacgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001815
VERSION     KHUT01001815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggtcaac
       61 taagtcggac gtgaaatccc tcggcttaac cggggaactg cgtccgatac tggatgactt
      121 gagttcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacactga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001816
VERSION     KHUT01001816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcttcg
       61 caagtcggat gtgaaatccc gtggctcaac catggacgtg cattcgaaac tgcgaagctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001817
VERSION     KHUT01001817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           93 g           48 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggtctag
       61 caagtcgggc atgaaatctc tgggctcaac ccggaggggt tgtccgatac tgctgggctt
      121 gagggcagca gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccacctggg ctgtacctga cgctcaaaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001818
VERSION     KHUT01001818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           86 g           45 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 taagttcgcc gtcaaatccc agggctcaac cctggacagg tggtgaaaac tactaagcta
      121 gagtacggta ggggcagagg gaatttccgg tggagcgatg aaatgcgtag agatcggaag
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001819
VERSION     KHUT01001819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcttct
       61 taagtcggat gtgaaatccc tcggctcaac cgaggacgtg cattcgaaac tgggaagctc
      121 gagtatgcaa gagggtcgtg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acgacctggg gcaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001820
VERSION     KHUT01001820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctat
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgtaac tgataggctg
      121 gagtgtggca gaggggggtg gaattccagg tgtagcagtg aaatgcgtag agatctggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001821
VERSION     KHUT01001821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccaag
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccgaaac tacatggctt
      121 gagtaccgga gaggatggcg gaatacccgg tgtagaggtg aaattcgtag atatcgggtg
      181 gaacaccagt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001822
VERSION     KHUT01001822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcgtag
       61 taagtccgtg gtgaaagcca acagctcaac tgttgaactg ccatggatac tgctaggctt
      121 gagtacagac gaggtaggcg gaatggatag tgtagcggtg aaatgcatag atactatcca
      181 gaacaccgat tgcgaaggca gcttactaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001823
VERSION     KHUT01001823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggttagg
       61 taggtccgct gtgaaaactc gaggcttaac ctcgagctgt cggcggaaac catttaacta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001824             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001824
VERSION     KHUT01001824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           85 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgtgta ggcggtttga
       61 taagtcagtt gtgaaattcc ccggcttaac cgggtggact gcgattgata ctgttagact
      121 tgaatcaggt tgaggtaagc ggaatgtgac atgtagcggt gaaatgctta gatatgtcat
      181 agaacaccga ttgcgaaggc agcttgctgg gcttagattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001825             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001825
VERSION     KHUT01001825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggcg
       61 taagtcgggt gtgaaatccc agggctcaac cctggaactg cattcgatac tgcgtcgctt
      121 tgaggactgg agaggagact ggaattcacg gtgtagcagt gaaatgcgta gatatcgtga
      181 ggaaggccag tggcgaaggc gagtctctgg acagttcctg acgctgaggc acgaaggcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01001826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001826
VERSION     KHUT01001826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccga
       61 taagtccgct gtaaaagtca aaggctcaac ctttgaaagc cggtggatac tgtcgggctg
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat agcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001827
VERSION     KHUT01001827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctgg
       61 taagtcggtc gtgaaatccc ttggcttaac caaggaactg cgtccgaaac tgttagactt
      121 gagtatcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001828
VERSION     KHUT01001828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcggtc gtgaaagccc ggggctcaac cccggagggt cgaccgatac tggcagacta
      121 gagtacagaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ctgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001829
VERSION     KHUT01001829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtccgta ggcggtcggt
       61 taagtcggat gtgaaattcc cgagcctaac tcggaaaggt cattcgaaac taatcggcta
      121 gagggctgga gaggagactg gaattgtcgg tgtagcggtg aaatgcgtag agatcgacaa
      181 gaacaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001830
VERSION     KHUT01001830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggac gtgaaagctc agggcttaac cctgagtctg cgttcgatac gggcaggcta
      121 gaggcaggta ggggagaacg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001831
VERSION     KHUT01001831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcccga
       61 taagtccgct gtgaaagtca agggcttaac ccttgaatgc cggtggaaac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001832
VERSION     KHUT01001832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           95 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaga
       61 aacgtgtgat gtgaaagccc cgggcttaac ctgggaaggg catcgcaaac ggactaactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaagcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001833
VERSION     KHUT01001833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           92 g           49 t
ORIGIN      
        1 aacgtaggtt ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtttgg
       61 caagtcgggc atgaaatctc tcggctcaac cgggagaggt tgtccgatac tgctggactt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccacctgga ctgcacctga cgctgacacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001834             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001834
VERSION     KHUT01001834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           93 g           45 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagacgc cacccgatac tgctgtgact
      121 agagtgcggt aggggagcgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc gccgctctgg gccgtgactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001835             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001835
VERSION     KHUT01001835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           94 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggtggcttcg
       61 taagtcgggt gtgaaaaccc caggctcaac ctggggacgc cactcgatac tgcggtggct
      121 tgagtctggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtgatctgg gccagtactg acactgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001836             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001836
VERSION     KHUT01001836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggctcag
       61 ttagtcggct gtgaaatccc agggctcaac cctggaactg cagtcgatac tgctgtggct
      121 agagtttggt agaggagaat ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccaatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001837             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001837
VERSION     KHUT01001837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcggct gtgaaagccc gaggctcaac ctcggaaatg cagtcgatac tgctgtggct
      121 agagtccggt agaggagagt ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggctctctgg gccggcactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01001838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001838
VERSION     KHUT01001838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           90 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcggcg gtgaaagtct gtggctcaac catggaactg ccgttgatac tggcaggctt
      121 gagtgccggt ggggtacatg gaattggtgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgtaggca ttgtacttac cggcaactga cgctgaggca cgaaagcgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01001839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001839
VERSION     KHUT01001839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          105 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagggtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgccgac tgccatgctg
      121 gagcactgta gaggcaggta gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaataccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001840
VERSION     KHUT01001840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agggctcgta ggcggtccgt
       61 cacgtcggga gtgaaatcct ggggctcaac cccagaactg ctttcgatac gggcggacta
      121 gaggtaggta ggggagaatg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001841
VERSION     KHUT01001841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           80 g           61 t
ORIGIN      
        1 tacgtaggac gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcgggcctt
       61 taagtcagtg gtgaaagttt gccgcttaac ggtaagattg ccattgatac tggaggcctt
      121 gagtgcagat gaggtaggcg gaatgtgtag tgtagtggtg aaatacttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactaaa ttgcaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001842
VERSION     KHUT01001842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           88 g           49 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtaga gtgaaatctc tgggctcacc ccagaaaccg ctctgcatac tggcgagctg
      121 gagtgtcgga gaggtatgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcatactggc cgaccactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001843
VERSION     KHUT01001843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattta ttgggcgtaa agggtgcgta gacggtttgt
       61 taagtctgct gtcaaattct ctgacttaat cggaggtagg cggtggaaac tggcaatcta
      121 gagtgtggaa gagaggagtg gaattcccgg agtagcggta aaatgcacag atctcgggag
      181 gaacaccaat ggcgaaggca gctccttggt ccactactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001844
VERSION     KHUT01001844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          111 g           34 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggccccg
       61 tgcgcgtgcc gtgaaagccc ggggctcaac cccgggtcgg cggtgcggac ggcggggctg
      121 gagcacatca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001845
VERSION     KHUT01001845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggttcca
       61 taagtgggat gtgaaagctc ctggctcaac tgggagaggt cgttccagac tgtgggacta
      121 gaggatggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccatttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001846
VERSION     KHUT01001846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 taagtcaggt gtgaaaaatc tgggctcaac ccagtggagc acctgatact gcggtgactc
      121 gagtccggta ggggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgac ggcgaaggca gcactctggg ccggtactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01001847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001847
VERSION     KHUT01001847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 tcagtcggac gtgaaagccc caggcttaac ctgggaactg cgtccgatac tggcaggctt
      121 ggattcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgagatcga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001848
VERSION     KHUT01001848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           98 g           41 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcggac gtgaaatccc ctggctcaac cagggaactg cgtccgatac tggcgggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001849
VERSION     KHUT01001849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggccgtc
       61 cacgtcggat gtgaaagctc ggagctcaac tctgagaggc cattcgatac gagacggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001850
VERSION     KHUT01001850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggcttca
       61 taagtcttgg gtgaaagccc tcggctcaac cgaggaattg ccttggatac tgtgaagctc
      121 gaggctggaa gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001851             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001851
VERSION     KHUT01001851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           82 g           64 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctgat
       61 taagtcagcg gtgaaaggta gcagcttaac tgttttacat gccgttgata ctggttagct
      121 tgagttaaca gaaggcagat agaatttctg gtgtagcggt gaaatgctta gataccagga
      181 ggaataccga ttgcgaaggc agtctgctgc agttacactg acgctgaggc acgaaagtgc
      241 ggggatcaaa cagg
//
LOCUS       KHUT01001852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001852
VERSION     KHUT01001852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           95 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacct catgcttaac atgtggcttg ctttcgatac gggcagacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001853
VERSION     KHUT01001853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgtgca ggtggtttgt
       61 taagtctgct gtgaaagccc cgggctcaac ctgggaatgg cagtggagac tggcaagcta
      121 gagtgcggta gaggatagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga ccagcactga cactcagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001854
VERSION     KHUT01001854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           75 g           64 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggggcag
       61 taagtcagtg gtgaaagtct gtggctcaac catagaattg ccattgatac tgttgtcctt
      121 gagttgttat gaagtggtta gaatgtgtag tgtagcggtg aaatgcatag atattacaca
      181 gaataccgat tgcgaaggca gatcactaat aacatactga cgctgatgga cgaaagcgta
      241 ggtagcgaac agg
//
LOCUS       KHUT01001855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001855
VERSION     KHUT01001855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagttggat gtgaaaactc tgggcttaac ccagagcctg cattcaaaac tgacaggcta
      121 gagttctgga gggggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagaaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001856
VERSION     KHUT01001856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggcttg
       61 taagtcagtg gtgaaatctc tgggcttaac ccggaaactg ccattgatac tataggtctt
      121 gaattctctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactacg gagacattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001857
VERSION     KHUT01001857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 ccagtcagat gtgaaagccc ggggctcaac tccggaattg catttgatac tgccaagctg
      121 gaggtcggaa gaggagggtg gaatatccag tgtagaggtg aaattcgtag atattggatg
      181 gaacaccggt ggcgaaagcg gctctctggt ccgaacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001858
VERSION     KHUT01001858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           86 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtctgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcttgaaac taccagactc
      121 gagggagata gaggaaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctttctggg tcttacctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001859
VERSION     KHUT01001859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           45 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtggac
       61 taagtcgggt gtgaaatctc cgggctcaac ccggagcgtg cgctcgaaac tggcccgcta
      121 gaggatggga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga ccattcctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001860
VERSION     KHUT01001860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 ttagtgaggg gtgaaatccc agggctcaac cctggaactg cctctcatac tggcagtctc
      121 gagtccggaa gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001861
VERSION     KHUT01001861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtttgc
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tggcagactc
      121 gagagcagta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ctgtatctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001862
VERSION     KHUT01001862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctgt
       61 taagtctgat gtgaaatccc ctcgctcaac gagggaactg cgtcggatac tggcagactt
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001863
VERSION     KHUT01001863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgga
       61 taagtcgagt gtgaaagccc ctggctcaac cagggagggt cattcgatac tgttcgactc
      121 gaaggcagga gagggaagtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001864             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001864
VERSION     KHUT01001864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           99 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcttat
       61 caagtcactt gtgaaatctc cgggcttaac ccggcagagg ccaagtgaaa ctgaggagct
      121 tggagtgcgg aaggggccat cggaattctt ggtgtagcgg tgaaatgcgt agatatcaag
      181 aggaacacca gcggcgaagg cgggtggctg ggccgacact gacgctgagg cgcgaaagct
      241 aggggagcga acggg
//
LOCUS       KHUT01001865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001865
VERSION     KHUT01001865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcccga
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgtcgggctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001866
VERSION     KHUT01001866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcaag
       61 taagtcagtg gtgaaatctc caggcttaac ctggaaactg ccattgatac tatttgtctt
      121 gaattgcgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cgttgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001867
VERSION     KHUT01001867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           86 g           48 t
ORIGIN      
        1 tacgaaaggt ccaagcgtta ctcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 tatgtcgatt gtgaaagccc cgggctcaac ctgggaactg cagtcgaaac tgcaaagctt
      121 gaatgttgga gaggttgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgactggc caaacattga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001868
VERSION     KHUT01001868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           84 g           51 t
ORIGIN      
        1 gacagaggtc ccgagcgtta gacggaatca ctgggcttaa agcgtgtgta ggcggatggg
       61 taagtacctt gtgaaatccc acagctcaac tgtggaactg cttggtatac tgcctgtctt
      121 gagccactta ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001869
VERSION     KHUT01001869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccttc
       61 taagtccgat gtgaaagccc ggggctcacc cccggaagtg cattggatac tgggaggctg
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001870
VERSION     KHUT01001870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c          104 g           41 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtac agggagcgta ggcggtttgg
       61 taagccctcc gggaaatctt cgggcttaac ccggaaaggt cggaggggac tgccaggcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgatcctga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001871
VERSION     KHUT01001871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtcgg
       61 tatgtcggac gtgaaatccc taggctcaac ctaggaactg cgctcgaaac tgccggtctt
      121 gagtggtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gcttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001872
VERSION     KHUT01001872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           93 g           47 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctg
       61 ccagtctgtg gtgaaatcct ccggctcaac cggagaactg cctcggatac tgcgggtctc
      121 gagggtgata ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga tcacttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001873
VERSION     KHUT01001873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcg
       61 caagtcaggg gtgaaatctt tcagcttaac tggaaaacag cccctgatac tgcgagtctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001874
VERSION     KHUT01001874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           96 g           47 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcaagg
       61 caagtcaggt gtgaaagccc cgagcttaac tcgggaattg catctgaaac tgctgtgctt
      121 gagttctgga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgc ggcgaaggcg gctacctgga cagaaactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001875
VERSION     KHUT01001875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           38 c           72 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttaa
       61 taagtcagtg gtgaaatctg gtcgctcaac gatcaaacgg ccattgatac tgttagactt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactatc aatatattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001876
VERSION     KHUT01001876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 tacgtcggaa gtgaaaaccc ggggcttaac cccgggcttg ctttcgatac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccactactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001877
VERSION     KHUT01001877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctctt
       61 taagtcagtg gtgaaagacc ccggctcaac cggggcagtg ccattgatac tggggagctt
      121 gagtgttgga ggggtacatg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgaaggca ttgtactggc caacaactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001878
VERSION     KHUT01001878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggagcgta ggcggctaaa
       61 taagtggggt gttaaatctc ccggctcaac cgggaacctg cacctcatac tgcttagctt
      121 gagtatagga gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggc ctaatactga cgctgatgct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001879
VERSION     KHUT01001879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           80 g           57 t
ORIGIN      
        1 tacggaggat gcgagtgtta tccggaatca ctgggcgtaa agcgtctgta ggttgtttgc
       61 taagtctgct gttaaagctt gaagctcaac ttcaaaaatg cagcggaaac tagtagactt
      121 gagtgtggta ggggtagagg gaatttccag tgaagtggtg aaatgcgtag atattggaaa
      181 gaccaccaat ggcgaaggca ctctactggg ccactactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01001880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001880
VERSION     KHUT01001880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatccc caagcttaac ttgggaactg ccattgatac tatttgtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctacg cggatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001881
VERSION     KHUT01001881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcatgca ggcggtaagc
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tggcttactg
      121 gaggctggta ggggaaagtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gctttctgga ccacgcctga cgctcagatg cgacagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001882
VERSION     KHUT01001882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagg
       61 caggtctggt gtgaaaactc gaggctcaac ctcgagaggt catcggaaac cgcttggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001883
VERSION     KHUT01001883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatatt
       61 taagtcaggg gtgaaatccc gcagctcaac tgcggaactg cctttgatac tgggtatctc
      121 gagtatggaa gaggtgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gctcactggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001884
VERSION     KHUT01001884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           84 g           51 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggttacc
       61 caagtcagag gtgaaatccc ggagctcaac tccggaactg cctttgaaac tatgtaacta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001885
VERSION     KHUT01001885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 taagccagat gtgaaatccc cgggcttaac ctgggaatgg catttgggac tgcacggcta
      121 gagtacggca gaggagacta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca ggtctctggg tcgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001886
VERSION     KHUT01001886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           92 g           50 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agggcgtgca ggtggttgag
       61 taagttggtt atgaaagctc ctggctaaac tgggagaggt tgaccaagac tgctgaactt
      121 gagtggatga gaggtaaacg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gtttactggc atccaactga cactgagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01001887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001887
VERSION     KHUT01001887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgcc
       61 tcagtcagac gtgaaatccc caggctcaac ttgggaactg cgtctgatac tgggtggctg
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgaaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001888
VERSION     KHUT01001888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgcag
       61 caagtcactt gtgaaatctc cgggcttaac ccggagcctg caggcgaaac tgctgtgctg
      121 gagtatggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001889
VERSION     KHUT01001889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           83 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcta
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgaggtg ccattgatac tgaagaactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001890             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001890
VERSION     KHUT01001890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           79 g           60 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaatcct gccgcttaac ggtagaattg ccattgatac tgagaagctt
      121 gagtacggtt gaggtaagcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gcttactaaa ccggcaactg acgctgatgc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001891
VERSION     KHUT01001891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtccgta ggcggtcggt
       61 taagtcggat gtgaaatctc acagcctaac tgtgagcggt cattcgaaac taatcggcta
      121 gagggctgga gaggagactg gaattgtcgg tgtagcggtg aaatgcgtag agatcgacaa
      181 gaacaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001892
VERSION     KHUT01001892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           86 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 taagtctgac gtgaaatctc caagctcaac ttggaaactg cgtcggatac tatccggcta
      121 gaggatcgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga cgattcctga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001893
VERSION     KHUT01001893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          101 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccaa
       61 ggagtcggat gtgaaagccc ggggctcaac tccggaggtg catccgatac tcttgggctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001894
VERSION     KHUT01001894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggttcgc
       61 taagttggat gtgaaaacta tgggctcaac ccatagcctg catccaatac tgacgaacta
      121 gagttctgga gggggtagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagagtctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001895
VERSION     KHUT01001895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgcgta ggcggcgagg
       61 caagttggat gtgaaagccc tgggctcaac ctaggatgtg catacaaaac tgcctcgctt
      121 gaatctcgga gggggtcagg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg ctgacctgga cgatgattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001896
VERSION     KHUT01001896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           85 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgcagggctt
      121 gagtagagtt gaggttggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat tgcgaaggca gctaactaag ctcaaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001897
VERSION     KHUT01001897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttcg
       61 taagtctgat gtgaaatccc cgggcttaac ctgggaattg cattggagac tgcgaggcta
      121 gaatctggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaagattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001898
VERSION     KHUT01001898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           98 g           40 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcggat gtgaaaacca gaggctcaac ctctggccgt cattcgatac tgccaggctc
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01001899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001899
VERSION     KHUT01001899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          101 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 tgcgtcggct gtgaaaaccc gcagctcaac tgtgggcttg cagctgatac gggcgggcta
      121 ggattcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatatcga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001900
VERSION     KHUT01001900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcgggacag
       61 taagtcaggg gtgaaagcct acggcttaac cgtagaactg cctttgatac tgctgatctt
      121 gagtcctgga gaggtcgctg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga ggtgtagacg ggcgactgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001901
VERSION     KHUT01001901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtttgat gtgaaagctc cgagcttaac tcggaaatgg cattgaatac tatcttgctc
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactaaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001902             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001902
VERSION     KHUT01001902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 caagtcggat gtgaaatctc cacgctcaac gtggaggggc cattcgaaac tgtcgtgact
      121 tgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgtgactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001903
VERSION     KHUT01001903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtctcgt gtgaaagctc gcagcttaac tgcgaaaagt cacgggatac tatccggcta
      121 gaggcttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01001904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001904
VERSION     KHUT01001904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggtggtttcg
       61 taagtctggc gtgaaatttc tcggcttaac cgaggcaggt cgtcggatac tgcggaactt
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cactgatgcg cgacagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001905
VERSION     KHUT01001905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 tcagtcggac gtgaaatccc tcggcttaac cggggaacgg cgtccgatac tggatggctt
      121 gaatccgaga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ttggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001906
VERSION     KHUT01001906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           60 c          104 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggttgcg
       61 agagcccggc gtgaaagccc ccagctcaac tgggggaggc cgtcggagac ctcgtgactt
      121 gaggccggta ggggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccggttctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01001907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001907
VERSION     KHUT01001907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 tacgtaggtc gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggctgat
       61 caagtccgac gtgaaagctc ccggcttaac tgggaaattg catcggaaac tggtcggctt
      121 gaaggtggga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ccactcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001908
VERSION     KHUT01001908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggaccat
       61 caagtcgggg gtgaaatccc ggggctcaac cccggaatgg ccttcgagac tggtggtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001909
VERSION     KHUT01001909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           80 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttgg
       61 taagtcagtg gtgaaagccc ttcgctcaac ggaggaacgg ccattgatac tgccagactt
      121 gaattactgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactacc ggtggattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001910
VERSION     KHUT01001910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgtct gtgaaaactc atggctcaac catgagcttg caggcgatac gggcagactt
      121 gagtattgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caaatactga cgctgaggag cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001911
VERSION     KHUT01001911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggatttg
       61 taagtagggg gtgaaatccc tgggctcaac ccaggaactg cctcctagac tgcaagtctt
      121 gagatcagga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actcgctgga ctgatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001912
VERSION     KHUT01001912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           86 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttttg
       61 caagtcgggt gtgaaatccc caagcttaac ttgggaactg catccgagac tgcatggcta
      121 gagtatggga gagagaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcttgga ccaatactga cgctcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001913
VERSION     KHUT01001913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgcttcgctc
      121 gagtatcgga gaggaaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctttctgga cgattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001914
VERSION     KHUT01001914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggaccgt
       61 taagtcggat gtgaaatctc acagcctaac tgtgataggt cattcgaaac tagcggactc
      121 gaggattgga gaggagactg gaatagtcgg tgtagcggtg aaatgcgtag agatcgacta
      181 gaacaccggt ggcgaaggcg agtctctgga caattcctga cgctcaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001915
VERSION     KHUT01001915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           93 g           43 t
ORIGIN      
        1 tacagagggt gctaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcttgg
       61 taagtgcggt gtgaaagccc ccggcctaac cggggaactg catcgcagac tgccaggctc
      121 gagagcggga ggggagactg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgaaaacg agtctctggt ccgcttctga cgctcaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001916             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001916
VERSION     KHUT01001916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcgggt gtgaaagccc cgagctcaac tcggggaggc cacccgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01001917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001917
VERSION     KHUT01001917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtcaagt gtgaaatccc ggggctcaac cccggaactg catttgatac tgacaagcta
      121 gagtgcggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga ctgacactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001918
VERSION     KHUT01001918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgatt
       61 taagtcagag gtgaaagccc ggggctcaac cccggaactg cctttgagac tggattgcta
      121 gaatcttgga gaggcgggtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcccgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001919
VERSION     KHUT01001919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggttggg
       61 catgtccgct gtgaaagcac acagctcaac tgtgttccgt cggcggaaac tgctcaactt
      121 gaggctggaa ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001920
VERSION     KHUT01001920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccga
       61 taagtcggta gtgaaagccc tcggcttaac cgaggaagtg ctatcgaaac tgtcggactt
      121 gaataccgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cggatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001921
VERSION     KHUT01001921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           84 g           57 t
ORIGIN      
        1 tacgtaggtc ccgagcgtta tccggaatta ttgggcgtaa agggtgcgca ggtggttatg
       61 taagtctggt gtgaaatttt tgggctcaac ccaaaaaggt cattggaaac tgcataactt
      121 gagagcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001922
VERSION     KHUT01001922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           88 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccat
       61 taagtcagcg gtgaaatccc acagctcaac tgtggaactg ccgttgatac tggtgggctt
      121 gagtaaagtc gaggcgggcg gaactggtgg tgtagcggtg aaatgcatag ataccaccaa
      181 gaaccccgat tgcgtaggca gctcgctagg ctttaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001923
VERSION     KHUT01001923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttga
       61 caagtccaag gtgaaatccc tcggctcaac cgaggaactg ccttggaaac tgtctggctc
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggatctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001924
VERSION     KHUT01001924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           85 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatgcg gcagcttaac tgtcgaactg ccattgatac tgcagggctt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacacctat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001925
VERSION     KHUT01001925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgg
       61 gaagttgggg gtgaaatccc ggggctcaac cccggaacgg cctccaaaac tccctgtctg
      121 gaggatggga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc ccatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001926
VERSION     KHUT01001926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttgc
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tggtcgacta
      121 gagtctggta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctaagactga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001927
VERSION     KHUT01001927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttttt
       61 caagcttgat gtgaaagccc cgggcttaac ctgggaatgg cattgaggac tgagaggcta
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccgacactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001928
VERSION     KHUT01001928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          106 g           38 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggcgct
       61 caagtcggtc gtgaaatctc cgggctcaac ccggcggggt cggccgaaac tgagtgtctt
      121 gagggaggca gaggagtctg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggactctggg cctaccctga cgctgaggcg cgaaggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001929
VERSION     KHUT01001929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 taagtcggaa gtgaaatctc aaggctcaac cttgaaatcg ctctcgatac tggcggactt
      121 gaggcaggta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001930
VERSION     KHUT01001930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           82 g           63 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtccta
       61 taagtcagtg gtgaaatatc gcagcttaac tgtgagggtg ccattgatac tgtaggactt
      121 gagtgaattt gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttaccaag cttcaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001931
VERSION     KHUT01001931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           82 g           64 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctat
       61 taagtcagtg gtgaaatatc gcagcttaac tgtgagggtg ccattgatac tgatggactt
      121 gagtgaagtc gaagtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg cttatactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001932
VERSION     KHUT01001932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           99 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgtc gtgaaagccc acagcttaac tgtgggtctg cggtggatac gggcagacta
      121 gaggcaggta ggggagaacg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001933
VERSION     KHUT01001933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           99 g           44 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggattga ctgggcgtaa agggcgtgta ggcggttgcc
       61 caagtccgtt gtgaaagccc gaggctcagc ctcggaagtg catcggaaac taggcagctg
      121 gagcgcgata gaggagggta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggc ggcgaaggcg gccctctgga tcgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001934
VERSION     KHUT01001934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttga
       61 taagtcacat gtgaaatctt cgggctcaac ccgaagtctg catgcgaaac tgtcgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001935
VERSION     KHUT01001935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           42 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtgcgc
       61 caagctcgat gtgaaagccc cgggcttaac ctgggaattg cattgaggac tggcgtgctc
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001936
VERSION     KHUT01001936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcttct
       61 caagtcaggg gtgaaagccc agagctcaac tctggaactg cctttgagac tgagaggctt
      121 gagtacggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc ccgttactga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001937
VERSION     KHUT01001937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtcagg
       61 caagtcgggt gtgaaagccc tgggctcaac ctgggaattg cattcgaaac tatctgactc
      121 gagtatggga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001938
VERSION     KHUT01001938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagcgtgcg gtgaaagctc gaggctcaac ctcgagtctg ccgtgcgaac tgccgagctg
      121 gagcacggta gaggcacatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgtgctggg ccgtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001939
VERSION     KHUT01001939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggctgcccct
       61 caagtggtgt atgaaagggc tcggctcaac cgggctaggt tatgccagac tgaggggcta
      121 gagagcgaga gcgggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcatctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001940
VERSION     KHUT01001940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          103 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggttcga
       61 taagtcgggg gtgaaatgct gcggctcaac cggagcatgg ccttcgagac tgtcgagctg
      121 gagcacggta gaggctggcg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaggcg accagctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001941
VERSION     KHUT01001941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggaatca ctgggcttaa agcgtacgta ggcggatctg
       61 taggcgtcgt gtgaaatacc tcggctcaac cgaggaactg cacggcgaac cacagttctt
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001942
VERSION     KHUT01001942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgcctgct gtgaaaaccc gaggctcaac ctcgggcttg cagtgggtac gggcagacta
      121 gagtgcggta ggggagaccg gaattcctgg tgtagcggta aaatgcacag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccgcaactga cgctgaggag cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001943
VERSION     KHUT01001943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgttt atcggaatta ctgggcgtaa agggtgcgta ggtggttcga
       61 taagtcagat gtgaaatccc tgggcttaac ctgggaattg catttgatac tgtcaaacta
      121 gagtgtggta gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcttactgga ccaacactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001944
VERSION     KHUT01001944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggcggctcgc
       61 taagtctgat gtgaaagccc cgggctcaac ccgggaagtg cattggaaac tggcgaactt
      121 gagtacggga gagggaagtg gaattccgag tgtaggggtg aaatccgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001945
VERSION     KHUT01001945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           85 g           54 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgta ggtggtttgt
       61 taagttgggt gttaaatctc tcggctcaac tgagaggtgt cgctcaaaac tagcagactg
      121 gaggatggta gaggatggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccatctggc ccattcctga cactcaaacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001946
VERSION     KHUT01001946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           91 g           42 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggcctgg
       61 aaagttggat gtgaaatctc tcggctcaac tgggagaggc cgttcaagac tatcaggctc
      121 gagggcgaca gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaga ggcgaaggcg acctcctggg tcgtacctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001947
VERSION     KHUT01001947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agagcacgta ggcggatcga
       61 tcagtcagag gtgaaagccc cgagctcaac ttgggaactg cctttgatac tgtcgatcta
      121 gagtccgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001948
VERSION     KHUT01001948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgtg
       61 caagtcggac gtgaaaaccc agagctcaac tctgggatgc cgttcgatac tgctcggcta
      121 gaggcaggta ggggaaagtg gaatacctgg tgtagcggtg aaatgcgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg gctttctggg cctcgcctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01001949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001949
VERSION     KHUT01001949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           74 g           62 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggtctta
       61 taagtcagtg gtgaaatctc tgggcttaac ccagaaattg ccattgatac tgtaggactt
      121 gagtatagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaaa ctataactga cactgaggct cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001950
VERSION     KHUT01001950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           42 c           74 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggacttg
       61 taagtcagtg gtgaaatctc ttcgcttaac gaagaaactg ccattgatac tgcaggtcta
      121 gagtatagat gacgttggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaaa ctataactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01001951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001951
VERSION     KHUT01001951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           80 g           56 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggttgtttaa
       61 aaagtctgca gttaaaggtt gaagcttaac ttcaaaaatg ccgtagaaac ttttaaacta
      121 gaggatggta ggggtagagg gaatttccag tggagcggtg aaatgtgtag atattggaaa
      181 gaccaccgat ggcgaaggca ctctactggg ccatgactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01001952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001952
VERSION     KHUT01001952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           94 g           51 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcgcat
       61 taagtctgac gtgaaatctc ccggcttaac tgggaggggt cgttggagac tgatgtgctt
      121 gaggcgatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg acaccctggc attagcctga cgctcatgtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001953
VERSION     KHUT01001953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          103 g           40 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgta ggcggtcttg
       61 tgcgtcggtg ctgaaagcgc ccggcttaac cgggcgaggc gtgccgatac gacgagactt
      121 gaggggcagc gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc tgctacctga cgctgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01001954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001954
VERSION     KHUT01001954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 gacgaactgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgga
       61 caagtcgggg gtgaaatcct gcagctcaac tgtagaagtg ccttcgatac tgttcgtctg
      121 gagggagata ggggtgtgtg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg acgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001955
VERSION     KHUT01001955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcgt
       61 taagtccggg gtgaaagccc actgctcaac agtggaactg ccccggaaac tggcggactt
      121 gagtacagac gaggttggcg gaatggaagg tgtagcggtg aaatgcatag ataccttcca
      181 gaaccccgat tgcgaaggca gctgactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001956
VERSION     KHUT01001956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           50 c           91 g           39 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggcttca
       61 caagtctcct gtcaaagatc agggctcaac cctggacagg caggagaaac tatgaggcta
      121 gagttcggta ggggcagagg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccaaa ggcgaaggca ctctgctggg ccgcaactga cactgagaga cgaaagcgag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01001957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001957
VERSION     KHUT01001957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           84 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttgc
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg cgtttgatac tggctgacta
      121 gagtttggta gagggaagtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccaat ggcgaaggca gcttcctgga ctaaaactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001958
VERSION     KHUT01001958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggcttgg
       61 caagtcggtt gtgaaatccc ggggcccaac cccggaactg cattcgaaac tgcctcgcta
      121 gagtcccgga gaggaaggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccttctgga cggtgactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001959
VERSION     KHUT01001959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           89 g           51 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgtgca ggtggcgcgg
       61 taagttgggc gtgaaatctc ccggctcaac tgggagaggt cgtccaatac taccgtgctt
      121 gagagcagaa gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctggt ctgtttctga cactcagacg cgacagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01001960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001960
VERSION     KHUT01001960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cacgtcaact gtgaaaactc ggggctcaac cccgagcctg cagtcgatac gggcagacta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgaaactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001961
VERSION     KHUT01001961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 tacagaggtg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttga
       61 caagtcccgg gtgaaatccc tcggctcaac cgaggaactg ccttggaaac tgtctggctt
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001962
VERSION     KHUT01001962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           82 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatact
       61 taagtaggaa gtgaaaggtg ggagctcaac tcccacattg ctccctatac tgggtgtctt
      121 gagtggcgga gagggagatg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca atctcctgga cgcaaactga cgctgagata cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01001963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001963
VERSION     KHUT01001963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgtcg
       61 aaagtttggc gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcgatgctg
      121 gagtgtggga aaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gcttgctaga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001964
VERSION     KHUT01001964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           92 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggtttgc
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccgaagac tggcaggctt
      121 gagtacggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001965
VERSION     KHUT01001965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggtctat
       61 caagtcagat gtgaaatatc ccggcttaac cgggaggggt catttgaaac tggtagacta
      121 gagtacagca gaggatagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actatctggg ctgaaactga cgctgaggcg cgaaggctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001966             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001966
VERSION     KHUT01001966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           44 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ttcggattta ctgggcataa agcgcgcgta ggcggctcga
       61 taagtcgggg gtgaaagccc gcagctcaac tgcggtgtgc cttcgaaact gtcgagcttg
      121 agtgcaggag gggtgattgg aactccaggt gtagcggtga aatgcgtaga tatctggagg
      181 aacaccagag gcgaaggcga atcactggac tgcaactgac gctgaggcgc gaaagctagg
      241 ggagcaaaca gg
//
LOCUS       KHUT01001967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001967
VERSION     KHUT01001967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagttggat gtgaaatctc ccggctcaac tgggagaggt cgttcaaaac tgccagactg
      121 gaggacggta gagggaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcctcctgga ccgtacctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01001968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001968
VERSION     KHUT01001968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agagcacgta ggtggtctgc
       61 taagtcgtgt gtgaaatccc ccggctcaac cggggaatag cgctcgatac tggcaggctc
      121 gaggtcggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga tcgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01001969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001969
VERSION     KHUT01001969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           98 g           42 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccca
       61 taagttccgc gtgaaatccc ccggctcaac cggggaatgg cgtggaaaac tgtggggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001970
VERSION     KHUT01001970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggccggttgg
       61 taagtcagat gtgaaagccc tgggctcaac ccaggaactg catttgaaac tgccagccta
      121 gagtggtgga gagggaggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gcctcctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001971
VERSION     KHUT01001971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           43 c           92 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggccttt
       61 taagtcagct ttgaaagctg gtcgcttaac gatcaggggt gggttgatac tggaaggctt
      121 ggatcaggtg gcggtttctg gaacgggtcg tgtagcggtg aaatgcatag atatgaccca
      181 gaactccgat tgcgaaggca ggaggctacg cctggattga cactgaggca cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001972
VERSION     KHUT01001972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgatg
       61 aaagttcgat gtgaaatctc cgggcttaac ccggaaaagg cattgaaaac tctgttgctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg ggtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001973
VERSION     KHUT01001973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtcgg
       61 aaagtcgggc gtgaaatccc caggctcaac ctgggaactg cactcgaaac ttccggtcta
      121 gagtggtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001974
VERSION     KHUT01001974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacaggc gtgaaatccc cgggctcaac ctgggaattg cgcttgtgac tgcaaggcta
      121 gagtgcgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg acgtgactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001975
VERSION     KHUT01001975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           85 g           50 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcatcg
       61 caagtcggat gtgaaatctt atggcttaac cataaaagtg catccgaaac tgcagtgctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001976
VERSION     KHUT01001976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           66 c          110 g           31 t
ORIGIN      
        1 tacggagggc gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gcagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tcccgcgctc
      121 gagcactgta ggggcgggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001977
VERSION     KHUT01001977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           92 g           43 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagccagat gtgaaagccc cgggctcaac ctgggaactg cgtttggaac tggcaagcta
      121 gagtacggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001978
VERSION     KHUT01001978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgat
       61 caagtcaggg gtgaaatcct gcagcttaac tgtagaagtg cctctgatac tgatcgtctg
      121 gagggagata ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg gcgcactgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001979
VERSION     KHUT01001979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacggagggg gctagtgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcttgg
       61 caagtcgagt gtgaaatccc ttggcttaac tgaggaacag cgctcgaaac tacctcgcta
      121 gaggacggga gagggaagtg gaactctcgg tgtaggggtg aaatctgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctggc ccgtagctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001980
VERSION     KHUT01001980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           93 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggctcta
       61 taagtatggt gtgaaatctc ccggcttaac tgggagggtg cgctatagac tgtggggcta
      121 gagtgtggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtaaacg gctttctgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01001981             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001981
VERSION     KHUT01001981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          100 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttag
       61 aaagcgtgtg gtgaaagccc ggggcttaac cccgggtctg ccgtgcgaac tactatggct
      121 agaggactgt agaggcaagt tgaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcttgctgg gcagtttctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001982
VERSION     KHUT01001982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 gacgagggat ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cattcgatac tgcgaagctt
      121 gagtgtagga gaggttacta gaattcttgg tgtagtggtg aaatacgtag atatcaagag
      181 gaataccgga ggcgaaggcg ggtaactggc ctaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001983             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001983
VERSION     KHUT01001983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           35 c           78 g           64 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtatgg
       61 taagttatta gtattatgtt gggctcaact caacggaagc tagtaatact gctttactag
      121 agagcaaggg aggttgacgg aattcgatgt ggagcggtga aatgctttga tatatcgagg
      181 aacaccaaaa agggcgaagg cagtcaacta tcttgtttct gacattgagg catgaaagct
      241 gggggagcaa aaagg
//
LOCUS       KHUT01001984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001984
VERSION     KHUT01001984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtctgt
       61 cgcgtccgtt gtgaaagccc agggctcaac cctgggtctg cagtggatac gggcagacta
      121 gagtcaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggaa cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01001985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001985
VERSION     KHUT01001985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggcagt
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tatctgtctt
      121 gaattatctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg ggtatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01001986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001986
VERSION     KHUT01001986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           55 c          105 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcat
       61 taagtggctg gtgaaatccc ggggctcaac tccggggctg ccggtcagac tggtgggctt
      121 gagcacggta gaggcggatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaataccggt ggcgaaggcg ttccgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001987
VERSION     KHUT01001987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           97 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggagcgta ggcggtgtgg
       61 caagtccgat gtgaaatctc cgggcttaac ccggagcggt cattggaaac tgccatgctc
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctgga ccacaactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01001988             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001988
VERSION     KHUT01001988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          103 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtggct
       61 taagcgtgcg gtgaaagctc ggagctcaac tccgagtcgg ccgtgcgaac tgggctcact
      121 ggagcatagt agaggcaggt ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcctgctgg gctatggctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01001989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001989
VERSION     KHUT01001989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggattgg
       61 tcagttaggg gtgaaatccc agagctcaac tctggaactg cccttaatac tgccagtcta
      121 gagtccggaa gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001990
VERSION     KHUT01001990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcacgca ggcggtcttc
       61 taagtctgat gtgaaatccc acggcttaac cgtggaaggt cattggaaac tggaggactt
      121 gagtgcagaa gaggagagtg gaattccacg tggagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggtg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01001991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001991
VERSION     KHUT01001991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          105 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcctgg
       61 taagcgtgcg gtgaaagcct ggggctcaac cccgggtcgg ccgtgcgaac tgctaggctt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001992
VERSION     KHUT01001992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           87 g           52 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttgg
       61 taagttgggt gttaaatctt tcggctcaac cgagaggtgt cgctcaagac tcccagactg
      121 gaggacggta gaggtaggca gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccagt ggcgaaggcg gcctactgga ccgttcctga cactcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01001993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001993
VERSION     KHUT01001993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           74 g           61 t
ORIGIN      
        1 tacggaggat gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggaaaat
       61 taagtcagcg gtgaaagctt acagcttaac tgtaaaaatg ccattgaaac tggttttctt
      121 gagttcgatt gaggtagatg gaatgtacca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaggca gtctactaag tcgatactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01001994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001994
VERSION     KHUT01001994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           96 g           43 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggcttaa
       61 taagttggac gtgaaagctc ccggcttaac tgggagaggc cgttcaaaac tgtaaggcta
      121 gagtgaggca gaggagcgcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcgctctggg cctaaactga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01001995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001995
VERSION     KHUT01001995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtcgtt
       61 caagtggtag gtgaaatccc tcggctcaac cgaggagctg cccgccaaac tgaacggctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggcactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01001996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU1999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001996
VERSION     KHUT01001996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU1999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          102 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtttgc
       61 taagtcactt gtgaaatccc ggtgctcaac gccggggctg caggtgagac tggcagactg
      121 gagctcggta gaggcgaatg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gttcgctggg ccgatgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01001997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001997
VERSION     KHUT01001997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           85 g           50 t
ORIGIN      
        1 tacagagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggtcttt
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tgggagactc
      121 gagggtaggc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01001998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001998
VERSION     KHUT01001998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           93 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaataa ttgggcgtaa agcgtccgta ggtggctgtt
       61 caagtctgcc gttaaagacc ggggcttaac cctggaaagg cggtggaaac tgaacggcta
      121 gagtgcagta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctgctgga ctgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01001999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01001999
VERSION     KHUT01001999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggacagt
       61 taagtcagag gtgaaagccc agggctcaac cctggaattg cctttgatac tgattgtctt
      121 gagtatggga gaggtatgcg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcatactggc ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002000             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002000
VERSION     KHUT01002000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           63 c           99 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtccgac gctgaaatcc cccggctcaa ccggggaagt ggcggggata cgaccggcct
      121 cgaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002001
VERSION     KHUT01002001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcttgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaactg cgctccaaac tcccaagctt
      121 gaggaagcta gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg acttttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002002
VERSION     KHUT01002002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcgggtcgc
       61 taagtcaggg gtgaaatgcc gaggctcaac ctcggaactg cctttgatac tgacgacctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002003
VERSION     KHUT01002003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           83 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggacttt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tattggtctt
      121 gaatgttgtt gaggtgggcg gaacgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca gcccgctaag caactattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002004
VERSION     KHUT01002004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacgtagggg acaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggccaag
       61 taagtcttac gtgaaatcct gaggctcaac ctcaggcctg cgcgggatac tgcttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacatccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002005
VERSION     KHUT01002005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttggt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaactg cgatggaaac tggctgacta
      121 gagtgtggta gagggtagcg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatccat ggcgaaggca gctacctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002006
VERSION     KHUT01002006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taggtccgtt gtgaaaactg gaggcttaac ctccagctgt cgatggaaac catttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002007             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002007
VERSION     KHUT01002007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggtgggtcgt
       61 taagttgagg gtgaaatccc agggctcaac cctggaactg ccttcaatac tggcgacctt
      121 cgagtccggg agaggtgagt ggaactgcga gtgtagaggt gaaattcgta gatattcgca
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acactgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002008
VERSION     KHUT01002008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggatcgc
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaattg cctttgatac tggcaatctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002009
VERSION     KHUT01002009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgcc
       61 tcagtccgac gtgaaatccc tcggctcaac cgaggaactg cgtcggatac tgggtggctt
      121 gaatccaaga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ttggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002010
VERSION     KHUT01002010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatta ttgggtgtaa agggtgtgca ggcgggactg
       61 taagtcagtg gtgaaagcct acggctcaac cgtagaattg cctttgatac tgcagtcctt
      121 gagtcccgga gaggccacta gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaataccaga agcgtaggcg ggtggctgga cgggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002011
VERSION     KHUT01002011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           97 g           44 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggctcgg
       61 taagtcggtc gtgaaatcct ggggctcaac cccaggactg cgtccgatac tgccgggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002012
VERSION     KHUT01002012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           95 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagcgcgca ggcggccgag
       61 caagtcggtt gtgaaatccc gtggctcaac cacggaaccg catccgaaac tgctcggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002013
VERSION     KHUT01002013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggtagcg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaatgg cattcgagac tgctctgcta
      121 gggtgcggaa gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gctccctggt ccagcaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002014
VERSION     KHUT01002014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           98 g           43 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggcggcccgt
       61 taagtctgcg gtgaaatccc ggggctcaac tccggatatg ccgcggatac tggcgggcta
      121 gagactggta gaggcgagtg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccagttctga cgctcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002015             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002015
VERSION     KHUT01002015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gcaagtccga agtgaaatct ccttgctcaa cgagggggct gccttggata ctgcggatct
      121 ggagaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002016
VERSION     KHUT01002016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaatctc cgggcctaac ccggaaactg catcggatac tattcggctg
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002017
VERSION     KHUT01002017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtttgg
       61 taagtgagat gtgaaatgct ccggctcacc cggggacctg catctcaaac tgctggacta
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002018
VERSION     KHUT01002018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggtggtctgt
       61 taagtcaggc gtgaaatcct cgggctcaac ccgagaactg cgtttgatac tgatagactc
      121 gagtgtggta gaggatagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctatctgga ccaacactga cactgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002019             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002019
VERSION     KHUT01002019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           43 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gtaagtcgga agtgaaattt cggggctcaa ccccgaagct gcttctgata ctgcggatct
      121 ggggaccggt agaggctggt ggaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaacacccg tggcgaaggc ggccagctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002020
VERSION     KHUT01002020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           98 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcttga
       61 taagtagggg gtgaaattcc atggctcaac catggatcgg ccttctagac tgtcaggctt
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002021
VERSION     KHUT01002021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttat
       61 caagtgagat gtgaaatccc cgggcttaac ctgggaactg catcttaaac tggtaagctg
      121 gagtactgta gaggacagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002022
VERSION     KHUT01002022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           90 g           51 t
ORIGIN      
        1 gacggaggtc ccgagcgttg ttcggaatta ttgggcttaa agcgcgtgta ggcggagccg
       61 taagtacctt gtgaaatccc cggactcaat ccgggaactg cttggtatac tgcgattctc
      121 gaggcaggta gaggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01002023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002023
VERSION     KHUT01002023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           73 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaatga
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaattg ccattgatac tgtcaatctt
      121 gagtacattt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01002024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002024
VERSION     KHUT01002024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacgggggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgattttg
       61 taagtctcgt gtgaaagccc aaagcctaac tttggaagtg cacgagatac tgtgaaattt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002025
VERSION     KHUT01002025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgta ggcgggcatt
       61 taagtccgtg gtgaaagccc tcagctcaac tgaggaactg ccatggatac tatttgtctt
      121 gaatgtcgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcttag atatgacccg
      181 gaacaccaat tgcgaaggca gcaggctaaa cgaatattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002026
VERSION     KHUT01002026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c           98 g           50 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 tgcgtcaacc gtgaaaactt gtggctcaac tacaagcttg cggttgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002027
VERSION     KHUT01002027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatta ctgggcttaa agcgcacgta ggcggtgctg
       61 taagtgtcag gtgaaatccc acggctcacc cgtggaactg cctggcagac tgcagtacta
      121 gagtgtggta ggggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccacaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002028
VERSION     KHUT01002028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           73 g           61 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattta
       61 caagtcagtg gtgaaatctc ccagcttaac tgggaaactg ccattgaaac tgtttatctt
      121 gagtacattt gaggcgggcg gaatgtgtca tgtagaggtg aaattcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002029
VERSION     KHUT01002029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           45 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggaatca ctgggcttaa agcgtgcgta ggcggatgtg
       61 caggcgtcgt gtgaaagccc tcggctcaac cggggaattg cacggcgaac cgcacgtctc
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccaaa ggtgaagaca gaccgctggg cttgttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002030
VERSION     KHUT01002030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtatgca ggcggacttt
       61 taagtcgggg gtgaaatccc gaggctcaac ctcggaactg ccttcgatac tggaagtctt
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002031
VERSION     KHUT01002031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcgtga
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgtcgtgctt
      121 gagtgccgga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002032
VERSION     KHUT01002032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           87 g           46 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtgcgg
       61 aaagtcggat gtgaaatccc tcagctcaac tgaggaagtg catccgaaac ctccgcactt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002033
VERSION     KHUT01002033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccatg
       61 taagtcaggt gtgaaagccc caggctcaac ctgggaagtg cacttgatac tgcgtggctt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002034
VERSION     KHUT01002034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agggtacgta ggcggtccgg
       61 taagtggggt gtgaaatccc tcggctcaac cgaggaactg cgccccatac tgccggactt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002035
VERSION     KHUT01002035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           86 g           50 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaataa ctgggcgtaa agagcgtgtc ggcggtttgt
       61 taagtcaact gtaaaagaac tcggcttaac cgagtaaatg cgggtgaaac tggcagacta
      121 gagtacggga gagaaaggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccttttggc ccgtaactga cgcttaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002036             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002036
VERSION     KHUT01002036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggctatg
       61 taagtcagtt gtgaaatacc tcggcttaac cgaaggaagt gcgattgata ctgcgtagct
      121 tgaatcaggt tgaggtaggc ggaatgtgac atgtagcggt gaaatgctta gatatgtcat
      181 agaacaccga ttgcgaaggc agcttactgg gctttgattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002037             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002037
VERSION     KHUT01002037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           50 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctggcaggct
      121 ggaggaacgg agaggtgagt ggaattcctg gtgtaccggt gaaatgggtt gatatcagga
      181 ggaacaccga tggcgaaggc agctcactgg ccgttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002038
VERSION     KHUT01002038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcccgca ggcggcctgt
       61 taagttcaag gtgaaatctc ccggctcaac tgggagggtg cctcgaaaac tggcaggctt
      121 gagtcatgga gagggatgca gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccagt ggcgaaggcg gcatcctggc catgtactga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002039
VERSION     KHUT01002039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c           92 g           42 t
ORIGIN      
        1 gacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtgcctt gtgaaatcct gcggctcaac cgcagaactg ctgggcatac tggcgatctt
      121 gggccactca ggggtcactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggtgactggg ggtgtgccga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002040
VERSION     KHUT01002040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatatt
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tgggtatctt
      121 gagtgtggta gaggtgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gctcactgga ccacaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002041
VERSION     KHUT01002041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           90 g           53 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctttt
       61 taagtcagag gtgaaatccc ggggctcaac tccggaactg cctttgagac tggatcgctt
      121 gaacgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgttga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002042
VERSION     KHUT01002042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           88 g           54 t
ORIGIN      
        1 tacgtaggga gcgagcgtta tccggattta ctgggtgtaa agggtgcgta ggcggtttgt
       61 taagtcagaa gtgaaattta ggggctcaac ctctaagctg cttctgaaac tgatgaacta
      121 gagtgtggga gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag agattcggag
      181 gaacaccagt agcgaaggcg gctttctgga ccataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002043
VERSION     KHUT01002043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggtctgg
       61 taagttggac gtgaaaactc cgggcttaac ttggagaggc cgttcaatac tgcgggactg
      121 gaggatgtca gaggggagtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggg gcatccctga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002044
VERSION     KHUT01002044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 tacggaaggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttcg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaactg cactcgatac tgcggagctc
      121 gagtacgaga gaggggggtg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacaccggt ggcgaaggcg gccccctggt tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002045
VERSION     KHUT01002045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           79 g           62 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggctttg
       61 taagtcagtg gtgaaagttt tcagcttaac tggaaaattg ccattgatac tgcagggctt
      121 gagtgcagat gaggtaggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactaaa ctgtaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002046
VERSION     KHUT01002046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          103 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggacgtt
       61 taagtgtggc ctgaaagccc ccggctcaac cggggatccg ggttgcaaac tgggcgtctt
      121 gagggggata ggggaaggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccttctggg tccgccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002047
VERSION     KHUT01002047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           92 g           52 t
ORIGIN      
        1 gacggaggtc ccgagcgttg ttcggaatta ttgggcttaa agcgcgtgta ggcggaacgg
       61 taagtacttt gtgaaatccc cggactcaat ccgggaattg ctgggtatac tgccgatctt
      121 gaggcaggta ggggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01002048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002048
VERSION     KHUT01002048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           82 g           62 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggtgggtttg
       61 taagtcagtg gtgaaatgct gccgcttaac ggtagaattg ccattgatac tgcaaatctt
      121 gaatatggtt gaggtaggcg gaatgtgttg tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactaag ccattattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002049
VERSION     KHUT01002049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtttga
       61 caagtctgat gtgaaatccc gcagcttaac tgcggaactg cattggaaac tgtttgacta
      121 gaggaatgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002050
VERSION     KHUT01002050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttctg
       61 caagtcaggc gtgaaatccc cgggcttaac ctgggaatgg cgcttgaaac tacgggactg
      121 gagtatggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002051             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002051
VERSION     KHUT01002051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           48 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcgggt gtgaaaactc taggctcaac ctagagacgc cacccgatac tgctatagct
      121 agaatccagt aggggagtgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccag cggcgaaggc ggcactctgg gctggtattg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01002052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002052
VERSION     KHUT01002052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggctgtcagt
       61 taagtctggt gtgaaagtca aaggctcaac ctttgaatgc cgctggaaac tggctgactt
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctgga acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002053
VERSION     KHUT01002053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           92 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgtgta ggcggtttac
       61 acagtcagat gtgaaatccc cgggcttaac ctgggagctg catttgatac gtgtagacta
      121 gagtgtgaga gagggttgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcaacctggc tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002054
VERSION     KHUT01002054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaatccc gaggctcaac ctcggaactg cactcgaaac tgcttggctc
      121 gagtcccgga gagggtggcg gaattcccgg tgtaggggtg aaatccgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002055
VERSION     KHUT01002055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggcggccggg
       61 tgtgtcgggt gtgaaagctc gaggcttaac ctcgaaactg cgcccgaaac tatccggcta
      121 gagtactgga gagggaagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaat ggcgaaggca gcttcctgga cagttactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01002056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002056
VERSION     KHUT01002056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatcct tgagcttaac tcgagaactg ccattgatac tatctgtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gcttactacg catatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002057
VERSION     KHUT01002057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c           98 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtttgt
       61 taagtgcagg gtgaaagctc ccggctcaac tgggagaggt ccttgcatac tggcgaactc
      121 gagggaggca gaggaaagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gctttctggg ccttacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002058
VERSION     KHUT01002058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c           99 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagcttgta ggcggctcgt
       61 cgcgtcgggt gtgaaaactt gcggcttaac cgcaagcctg cacccgatac gggcgggcta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgagaag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002059
VERSION     KHUT01002059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           99 g           36 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggcaccg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcggagctg
      121 gagcgatgca gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgactggg catcagctga cgctgaagtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002060
VERSION     KHUT01002060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggctgatagt
       61 taagtgaggg gtgaaatccc gaggctcaac ctcggaactg cctttcatac tggctgtctc
      121 gagtccggaa gaggttagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002061
VERSION     KHUT01002061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggatagt
       61 tcagtcagag gtgaaagccc agggctcaac cttggaactg cctttgatac tggctatctt
      121 gagtttggga gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggc ccaatactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002062
VERSION     KHUT01002062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatctgtctt
      121 gaatatcgtg aaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactgca cgattattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002063
VERSION     KHUT01002063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           93 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtatt
       61 caagtctatg gtgaaatccg gcagctcaac tgtcgaattg ccgtggatac tgggtatctc
      121 gagggaggta ggggcgtgcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002064
VERSION     KHUT01002064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggtggtttcg
       61 ttagtcggac atgaaagctc ctggcttaac tgggagaggt ggttcgatac tgcgagactt
      121 gaggttggga gagggacgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gcgtcctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002065
VERSION     KHUT01002065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           84 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggaagcg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgcgtttctt
      121 gagccaacta ggggctatcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca gatagctggg gttgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002066
VERSION     KHUT01002066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           94 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccgag
       61 caggtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac cgctcggctc
      121 gagtcccgga gaggaagttg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggc ggcgaaagcg gacttctgga cgaggactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002067
VERSION     KHUT01002067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcctgt
       61 tgcgtccgct gtgaaaactc agggcttaac cctgagccgg cagtggatac gggcaggcta
      121 gaggtaggta ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cactgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002068
VERSION     KHUT01002068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          108 g           36 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 tcagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgcggagctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002069             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002069
VERSION     KHUT01002069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggccttg
       61 taagtctgtt gtttaaactc ggggctcaac cccgagtcgc aatggaaact gcaaagcttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01002070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002070
VERSION     KHUT01002070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatta ctgggcgtaa agggtctgta ggcggacgtt
       61 taggtctggt gtgacatctc gcggcttaac tgcgagcgag cgccggaaac cggacgacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002071
VERSION     KHUT01002071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c          104 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggtcgtg
       61 tatgtgcttg gtgacatcct cccgcttaac gggaggcggc ccaggtagac agcgcgactg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002072
VERSION     KHUT01002072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           45 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtccgc
       61 taagtcagtt gtgaaatccc tcggctcaac cgaggaactg cacctgatac tggcgggctt
      121 gaggacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctcactgga ccgtatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002073
VERSION     KHUT01002073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggaccat
       61 caagttgggg gtgaaatccc ggggctcaac cccggaactg cctccaaaac tgctggtctg
      121 gaggtcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002074
VERSION     KHUT01002074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           63 c          112 g           34 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcggtc
       61 cgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggatcgctt
      121 gaggacggca ggggcaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcctgctggg ccgatcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002075
VERSION     KHUT01002075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgta ggcggtttgt
       61 caagacggat gtgaaatccc cgggcttaac ctgggcatgg catacgtgac tggcagactc
      121 gagtagggca gagggaggcg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ctcatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002076
VERSION     KHUT01002076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           79 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggttcgg
       61 tcagttgggt gtgaaaagtc gaggctcaac ctcgaaattg cacccaatac taccgggcta
      121 gagtactgca taggaagctg gaacttacgg tgtagcagtg aaatgcgtag atatcgtaaa
      181 gaagaccaga ggcgaaagcg agcttctagg cagttactga cgctcatgca cgaaagccaa
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002077
VERSION     KHUT01002077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           88 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggccttg
       61 caagtcgggc gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tgctcggcta
      121 gagtcttgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga caatgactga cgctaaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002078
VERSION     KHUT01002078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggctacg
       61 caagtcggat gtgaaaactc ggggctcaac cccgagcgtg catccgatac tgcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002079
VERSION     KHUT01002079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           97 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggcctgt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaatgg cggtggatac tggcgggctc
      121 gagtacggta gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccacctgga ccagtactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002080             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002080
VERSION     KHUT01002080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           81 g           55 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggattgt
       61 taagtgcaat gcgaaatcct gtggctcaac catatggact gtattgcata ctggcaatct
      121 agaggacatg agaggcaaat ggaattaccg gtgtagcagt gaaatgcgta gatatcggta
      181 ggaacaccaa tggcgaaggc agtttgctgg catgtatctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002081
VERSION     KHUT01002081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           74 g           64 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtg gtgaaagctc catgcttaac atggaaattg ccattgatac tgatgagctt
      121 gagtagattt gaagtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcactaaa ctcttactga cgctgaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01002082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002082
VERSION     KHUT01002082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           99 g           48 t
ORIGIN      
        1 tacagagggg gccagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctttg
       61 cgtgtctttc gtgaaatccc tcggcttaac tgaggacgtg cggaagaaac tgtagagctg
      121 gagtccggga gaggggggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccccctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002083
VERSION     KHUT01002083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcttga
       61 caagtcaggt gtgaaagccc cgggctcaac ccgggaattg cacttgatac tgtctcgcta
      121 gagtccggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002084
VERSION     KHUT01002084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtggtt
       61 caagttgggg gtgaaatccc ggggctcaac cccggaattg cctccaagac tggattgctg
      121 gagtccggaa gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ccgggactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002085
VERSION     KHUT01002085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           96 g           43 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtcta ggcggtcagg
       61 taagtgggat gtgaaatgcc ccggctcacc cggggaccgg catcccatac tgcttggctt
      121 gagtacggga gaggatgtgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cacatctggc ccgatactga cgctgaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002086
VERSION     KHUT01002086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcagcg
       61 caagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac tgcgtagctt
      121 gagttctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002087
VERSION     KHUT01002087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 tacgagaggc gcaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcgcag
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaattg cgctctaaac tactgtgctc
      121 gagggaccta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg ggtcttctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002088
VERSION     KHUT01002088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtctgcc gtgaaagccc cgggctcaac ctgggaattg cggtggatac tggcaagcta
      121 gagtacggta gaggatggtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctat ggcgaaggca gccatctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002089
VERSION     KHUT01002089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctcg
       61 caagtccgat gtgaaatccc cgggctcaac ctgggaactg cgttggaaac tacgaggctg
      121 gagtacggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctagtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002090
VERSION     KHUT01002090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctatt
       61 caggtcaggt gtgaaagccc ccggctcaac cggggaactg cgcttgaaac cgagtggctc
      121 gagtcagcta gaggtacacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgtactggg gctggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002091
VERSION     KHUT01002091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           97 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgaga
       61 tgagtgtgat gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac gatctgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002092
VERSION     KHUT01002092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 cacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 cacgtcggat gtgaaattcc agggctcaac cctggacctg cattcgatac gggcggacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002093
VERSION     KHUT01002093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggactgc
       61 acagtccgat gtgaaattcc tgggctcaac ctgggggctg cattggatac gtatggtctt
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002094
VERSION     KHUT01002094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c          100 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccgg
       61 taagtccgct ctgaaagtcc ggggctcaac cccgggaggc gggcggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002095
VERSION     KHUT01002095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgcgta ggcggattgt
       61 taagtcagtt gtgaaagccc tgggctcaac ctaggaacgg cgattgaaac tggcagtcta
      121 gagtttagta gagggaaggg gaatttctgg agtagcagtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaggcg ccttcctgga ctaaaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002096
VERSION     KHUT01002096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacgggggga gcgagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttgga
       61 taagtcagag gtgaaatccc agggcttaac cttggaattg ccttcgatac tgtccgacta
      121 gagggcggaa gaggatagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctatctggt ccgctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002097
VERSION     KHUT01002097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           92 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcctcg
       61 taagtccgct gtgaaagtca aaggctcaac ctttgaatgc cggtggatac tgcgaggcta
      121 gagtccggaa gaggcgagtg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccaac ggcgaaggca gctcgctagg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002098
VERSION     KHUT01002098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcttcg
       61 aaagtcaggt gtgaaatccc tgggctcaac ccaggaagtg cacttgaaac ttcgaagctc
      121 gagtcccgga gagggtggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctacctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002099
VERSION     KHUT01002099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacgaggggt gcaaacgttg tccggaatta ctgggcgtaa agagcgcgta ggtggtttgg
       61 taagtcgtct attaaagacc agggcttaac cctgggaatg tggacgaaac tactttactt
      121 gagtatggca ggggctagta gaacacttgg tggaggggtg acatccgtag atatcaagtg
      181 gaataccaag ggcgaaggca gctagctggg ccattactga cactgaggcg cgaaagcgtg
      241 gggagcgatc ggg
//
LOCUS       KHUT01002100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002100
VERSION     KHUT01002100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           56 c          104 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggtggtccgt
       61 cgcgtcgtgc gtgaaatacc ccggctcaac tggggggggt cgtgcgatac gggcggactg
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cactcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01002101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002101
VERSION     KHUT01002101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 ttagtcgggg gtgaaatccc ggggctcaac cccggaacgg cctccgatac tggcagtctg
      121 gagttcggga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002102
VERSION     KHUT01002102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           89 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaataa ctgggcgtaa agggtgcgta ggcggtatga
       61 aaagtctgat gtgaaatccc cgggctcaac ctgggaacgg cattggaaac tatcaagcta
      121 gagtgcgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg atgacactga cgctgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002103
VERSION     KHUT01002103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtggtg
       61 taagtttgat gtgaaatctc cgggctcaac ccggaaatgg cattgaatac tacattgctg
      121 gaggattgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caatacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01002104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002104
VERSION     KHUT01002104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcggg
       61 taagtcggat gtgaaatcct ggggctcaac ctcagaactg cattcgatac tgctcggcta
      121 gagtattgga gaggtgagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagc ggcgaaggcg gctcactgga caattactga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002105             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002105
VERSION     KHUT01002105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgcgg
       61 taagttaggc gtgaaaggcc cgggctcaac ccgggaactg cgcttaaaac tgccgcgctc
      121 tgagtccgag agaggatagc ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccag tggcgaaggc ggctatctgg ctcggtactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002106             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002106
VERSION     KHUT01002106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 taagtcgtga agtcaaatct ctgggctcaa cccagaatcg cttccgatac tgcggtgact
      121 ggagtccggt aggggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cagcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcat
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002107
VERSION     KHUT01002107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcactc
       61 taagtacgtc gtgaaatatc ccggctcaac cgggagggtg cgatgtagac tagggagcta
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002108
VERSION     KHUT01002108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttctc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggagggcta
      121 gagtgcggga tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctctctgga ccgcaactga cgctgaaaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002109
VERSION     KHUT01002109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          107 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtgcgg
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgctggactg
      121 gagcatgtca gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg acatagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002110
VERSION     KHUT01002110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcgggtcgt
       61 taagttgggg gtgaaatccc gaggctcaac ctcggaactg ccttcaaaac tggcgacctg
      121 gactcaagta gaggcaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg acttgctgga cttgtagtga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002111             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002111
VERSION     KHUT01002111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           90 g           44 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgggtcacgg gtgaaatcct ccagcttaac tggagaactg cccttgatac cactgcggct
      121 cgaggaagga aggggtcatg ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgccgg tggcgaaagc gcatgactgg tccttttctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01002112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002112
VERSION     KHUT01002112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           90 g           43 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcgctg
       61 taagtcggac gtgaaatccc tcggctcaac cgaggacgtg catccgaaac tacagtgctc
      121 gagtatggaa gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002113
VERSION     KHUT01002113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccttg
       61 caagttgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccaaaac tacgaggctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002114
VERSION     KHUT01002114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagccgatt gtgaaatccc cgggcttaac ctgggaactg cagtcgggac tgtctagcta
      121 gagtatcgga gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga cgaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002115
VERSION     KHUT01002115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgattcga
       61 taagtctcat gtgaaatccc agggcctaac cctggaatgg catgggatac tgttggattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002116
VERSION     KHUT01002116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 cacgggggga gcaaacgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctcg
       61 taagtggtgt gtgaaatgtc ctggctcaac cagggaattg cgctccaaac tgcgaggctt
      121 gagggcagta gaggaagctg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccatt ggcgaaggcg agcttctggg ctgtccctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01002117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002117
VERSION     KHUT01002117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttctg
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaattg cgtttgaaac tacaaggcta
      121 gagtgtagca gaggggggtg gaattccatg tgtagcagtg aaatgcgtag agatatggaa
      181 gaacatcgat ggcgaaggca gccccctggg ttaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002118
VERSION     KHUT01002118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttggc
       61 caagtctgat gtgaaagccc cgggctcaac ctgggaatgg cattggaaac tggctggcta
      121 gagtttggca gaggggggtg gaactcctgg tgtagcagtg aaatgcgtag agatcaggaa
      181 gaacaccgat ggcgaaggca gccccctggg ctggaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002119
VERSION     KHUT01002119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggtccgg
       61 taagtcagct gtgaaagtcc agggctcaac cctgggatgc cggctgatac tgccagacta
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002120
VERSION     KHUT01002120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggccaag
       61 taagttgggt gtgaaagccc tgggctcaac ccaggaagtg catccaaaac tgcttggctt
      121 gagtaccgga ggggttcaga gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaataccagt ggcgaaggcg ctgaactgga cggtaactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002121
VERSION     KHUT01002121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggtggttctt
       61 taagtctgat gtgaaagcct ccggctcaac cggagagggt cattggaaac tggggaactt
      121 gagtgcagaa gaggatagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actatctggt ctgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002122
VERSION     KHUT01002122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           98 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcacac
       61 acagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgtttgagac gtgtgagcta
      121 gaggatggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002123
VERSION     KHUT01002123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacggggggt gcgagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggtttgt
       61 gaagtctgat gtgaaagccc cgggcccaac ccgggaagtg cattggaaac tggcagactt
      121 gaatacggga gaggagagtg gaattcctgg tgtagaggtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002124
VERSION     KHUT01002124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgttac tggcagactt
      121 gagtgtggca gaggggggtg gaattccagg tgtagcagtg aaatgcgtag agatctggag
      181 gaacaccgat ggcgaaggca gccccctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002125
VERSION     KHUT01002125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcca
       61 caagtcactt gtgaaatccc caggcttaac ctggggcctg caggcgaaac tgtggtgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002126
VERSION     KHUT01002126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           84 g           52 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggttggg
       61 aaagttgggt gtgaaagccc tcggcttaac cgaggaattg cgctcaaaac tacctgacta
      121 gaggaagata gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002127
VERSION     KHUT01002127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           82 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 gaagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tctctgtctt
      121 gaatactgtg aaggtgagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccaat tgcgaaggca gctcactgcg cagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002128
VERSION     KHUT01002128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           98 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggtttgg
       61 acagtcagat gtgaaattcc tgggctcaac ctgggggctg catttgatac gtttggacta
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002129
VERSION     KHUT01002129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacgtaggtc ccgagcgttg tccggaatta ctgggtgtaa agggtgcgca ggcggccatg
       61 tgcgtcagaa gtgaaatatc acggcttaac cgtgagggtg cttttgaaac ggcgtggctt
      121 gagtacggga gaggatgatg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gtcatctggc ccggtactga cgctcaggca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002130
VERSION     KHUT01002130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggtctct
       61 cacgtccggg gtgaaagccc agagctcaac tctgggattg ccttggatac ggggagactt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002131
VERSION     KHUT01002131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggccgat
       61 taagtttgtc gtgaaatctc ccggcttaac tgggagggtg cggcgaatac tgaccggctc
      121 gagtgtggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002132
VERSION     KHUT01002132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggtcctg
       61 taagtggtgt gtgaaatccg atggctcaac catcgagctg cgccccatac tgcaggactt
      121 gagtacagca gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccgtt ggcgaaggcg actttctggg ctgctactga cgctgaggcg cgaaagccag
      241 gggagcgaac agg
//
LOCUS       KHUT01002133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002133
VERSION     KHUT01002133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggctttt
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaatgg cattcgatac tgaaaagcta
      121 gagtctggta gagggtggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gccacctgga ctgagactga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002134
VERSION     KHUT01002134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcgat
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt tgctcaagac tggtcgactg
      121 gagaatggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccatatctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002135
VERSION     KHUT01002135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 taagtccgtt gtaaaagtcg aaggctcaac cttcgaatgc cgatggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat agcgaaggca gctcgctggg acggtactga cgctaagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002136
VERSION     KHUT01002136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           81 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatttt
       61 taagtcagtg gtgaaatccc ggggctcaac cccggaactg ccattgatac tattggtctt
      121 gaatactgtg gaggtgagcg gaatgtgtca tgtagcggtg aaatgcatag agatgacaca
      181 gaacaccgat agcgaaggca gctcactacg cagtcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002137             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002137
VERSION     KHUT01002137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           50 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcccgt
       61 taagtgcaaa agtgaaagct ccaggctcaa cttggaaatt gctttgcata ctggcaggct
      121 ggaggaacgc agaggtctgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcagactgg gcgtttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002138             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002138
VERSION     KHUT01002138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggagcgta ggtggtcggg
       61 taagtcagat gttaaatttc gatgctcaac gtcgaatcgc atttgatact gcctggctag
      121 aggaattgag aggaggccgg aattcacggt gtagcagtga aatgcgtaga tatcgtgagg
      181 aagaccaacg gcgaaagcag gcctctggaa atctcctgac actgaggctc gaaggccagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01002139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002139
VERSION     KHUT01002139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           79 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcatg
       61 taagtcagtg gtgaaatcct agagcttaac tctagaactg ccattgatac tatgtgtctt
      121 gaatttcctg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctgactacg ggtagattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002140
VERSION     KHUT01002140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           83 g           57 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggggtga
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaattg ccattgatac tgtcattctt
      121 gagtacgctc gacgtaggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agtgaagaca gcttacgagg gcgaaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01002141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002141
VERSION     KHUT01002141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           89 g           58 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtttggt gttaaagatt ggggctcaac cctgaaactg cactgaatac tggcagactc
      121 gagtgtggta gaggctagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaggcg actagctggg ccataactga cactgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01002142             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002142
VERSION     KHUT01002142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttaat
       61 taagtctggt gtctaaggct gaggctcaac ctcagttcgc accggaaact ggttaacttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 gtagcaaaca gg
//
LOCUS       KHUT01002143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002143
VERSION     KHUT01002143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           94 g           44 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agggtgcgta ggcggcatgg
       61 caagtgagga gtgaaagccc ctggctaaac taggggagga catctcagac tgctaagcta
      121 gagtgaaaca gaggaacgtg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggtgcaaacg acgttctggg tttcaactga cgctgaggca cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002144
VERSION     KHUT01002144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttgt
       61 taagtcaggg gtgaaagact gcagctcaac tgtagcagtg cctttgatac tgatgagctt
      121 gagtatagat gaggccggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gccggctaaa ctacaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01002145             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002145
VERSION     KHUT01002145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           67 c          100 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtcgatg gctgaaatcc cccggctcaa ccggggaagt ggcctcgata cgaccggcct
      121 ggaggggggt agggggcgcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggcgcctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002146
VERSION     KHUT01002146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          104 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcccgg
       61 tcagtcagtg gtgaaagcgc ggggctcaac tccgcgtcgg ccattgatac tgctgggctt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002147             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002147
VERSION     KHUT01002147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           90 g           52 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggtctgt
       61 taagtgcaca agtgaaagct ccgggctcaa ctcggaaatt gctttgcata ctggcaggct
      121 ggagggacgc agaggtctgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcagactgg gcgtttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002148
VERSION     KHUT01002148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ctgggcgtaa agggttcgta ggcgggagct
       61 taagtcaagt gtgaaatccc taggctcaac ctaggacgtg cacctgaaac tgagcttctt
      121 gaatcttgga gagggtgatg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaggcg atcacctggc caaagattga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002149
VERSION     KHUT01002149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgacc
       61 caagtcgatg gtgaaagctc gccgctcaac ggcgagtcgg ccaccgatac tgggttgctc
      121 gagcactgta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002150
VERSION     KHUT01002150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggatcgc
       61 aaagttgggg gtgaaagccc agggctcaac cctggaattg ccttcaagac tcgcggtctg
      121 gagttcgaga gaggtgggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcccactggc tcgacactga cgctgaggcg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01002151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002151
VERSION     KHUT01002151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgat
       61 taagcttgct gtgaaagccc cgggcttaac ctgggaatgg cagtgaggac tggtcgactg
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002152
VERSION     KHUT01002152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgat
       61 taagtcggat gtgaaatccc ctggctcaac tggggggcgt cattcgatac tgctcgactt
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002153
VERSION     KHUT01002153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtctgtg gtgaaatctc taagcttaac ttggaaactg ccatggatac tatagatctt
      121 gaataccgtg gaggatagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctgtctacc cgaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002154
VERSION     KHUT01002154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcctgc
       61 taagtctgat gtgaaagccc gaggctcacc ctcggaagtg cattggaaac tggcagactg
      121 gagtcccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002155
VERSION     KHUT01002155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           77 g           61 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggataaa
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaattg ccattgatac tgtttatctt
      121 gagtacagct gaggtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002156
VERSION     KHUT01002156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          101 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcggac
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaggtg cgtctgggac tggtatgcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002157
VERSION     KHUT01002157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           80 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgca ggcggacttg
       61 taagtcagtg gtgaaatacc tgagcttaac tcgggaactg ccattgatac tataggtctt
      121 gaatattgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccaat tgcgaaggca gcaggctaaa caaagattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002158
VERSION     KHUT01002158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggtgttg
       61 tcagtcagac gtgaaatccc caggcttaac ttgggaactg cgtctgatac tacaagactt
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgaaactga cactgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002159
VERSION     KHUT01002159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccctt
       61 cgcgtcggat gtgaaagctc agggctcaac cctgagaggc cattcgatac gggagggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002160
VERSION     KHUT01002160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtggcc
       61 caagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tgggttgctt
      121 gagtactgga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggtgtagacg gcttgctgga cagtcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002161
VERSION     KHUT01002161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtccgat gtgaaagccc tgggctcaac ctgggaatgg cattggatac tggcaagcta
      121 gagtgcggta gaggggtgcg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatccgt ggcgaaggcg gcaccctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002162
VERSION     KHUT01002162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcggtg
       61 taagtctaac gtgaaatctc ggggctcaac ctcgaaattg cgtcggatac tgcattgcta
      121 gaggattgta gaggagagtg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt tgcgaaggcg actctctggg caactcctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002163
VERSION     KHUT01002163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggtccat
       61 caagtggggt gtgaaatccc tcggctcaac cgaggaattg cgctccaaac tgttgggctc
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccaga ggcgaaggcg gctcactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002164
VERSION     KHUT01002164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gtcggccact
       61 caagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgatac tgtgtggcta
      121 gagttcggca gagggaagtg gaatttcggg tgtagcggtg aaatgcgtag atatccgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002165
VERSION     KHUT01002165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcgggttgt
       61 caagttggag gtgaaagccc agggctcaac cttggaatag ccttcaaaac tgacagcctg
      121 gagtatggaa gaggtaagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acttactggt ccatgactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002166
VERSION     KHUT01002166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccttc
       61 caggtccggt gtgaaatctc acggctcaac cgtgaaacag cgctggaaac cggaaggctt
      121 gagtgtgcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg gcacaactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002167
VERSION     KHUT01002167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           82 g           60 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggcgggcctg
       61 taagtcagtg gtgaaatctt gccgcttaac ggtaaaattg ccattgatac tgcaggtctt
      121 gaatatggtt gaggtaggcg gaatgtgttg tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactaag ccattattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002168
VERSION     KHUT01002168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggtggccaac
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggatggctt
      121 gagtgtggga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccataactga cactgagaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002169
VERSION     KHUT01002169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           94 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtacga
       61 caagtcagga gtgaaagccc ggggctcaac cccggaactg cttttgaaac tgtcgaacta
      121 gagtgcagaa gaggcaaccg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002170
VERSION     KHUT01002170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          106 g           38 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtcgcg
       61 tatgtcggct gtgaaagccg ccggctcaac cggcggaggg cggcggaaac tgcgcgacta
      121 gaggctggga ggggctggtg gaattccccg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002171
VERSION     KHUT01002171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatcag
       61 taagtcagtg gtgaaatctc cgggcttaac tcggaaactg ccgttgatac tattgatctt
      121 gaatatcatg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttactaca tgttaattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002172
VERSION     KHUT01002172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggcttgg
       61 taagtcgggt gtgaaatcct ggggctcaac cccggaactg cacccgaaac tgcccggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002173
VERSION     KHUT01002173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcttcc
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg cgtttgaaac tgggaggcta
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccagcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002174
VERSION     KHUT01002174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggcttga
       61 taggtctgct gtgaaaactc ggggctcaac cccgagacgt cggtggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002175
VERSION     KHUT01002175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaaa
       61 caggtccact gtgaaaactc gaggctcaac ctcgagaggt cggtggaaac cgtctggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002176
VERSION     KHUT01002176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgctaca
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgcgtagctt
      121 gagtctcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaagactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002177             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002177
VERSION     KHUT01002177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 tgagtcggct gtgaaatctc cacgctcaac gtggaggggt cagtcgatac tgcggtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002178
VERSION     KHUT01002178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           97 g           40 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggcggccgat
       61 taagtccggt gtgaaagccc ggggctcacc cccggaagtg cgctggaaac tgatcggcta
      121 gagtacggga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gcttcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002179
VERSION     KHUT01002179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggctctg
       61 aaagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgatac tctggagctt
      121 gagtgtggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002180
VERSION     KHUT01002180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           91 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggctaga
       61 caagtctggt gtgaaaaccc ggggctcaac cctgggactg cattggaaac tgtttggctt
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002181
VERSION     KHUT01002181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggatcag
       61 caagtcaggg gtgaaatccc ggggctcaac cccggcactg cctttgagac tgttggtctg
      121 gagttcgaga gaggtgggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcccactggc tcgacactga cgctgaggcg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01002182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002182
VERSION     KHUT01002182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacagagggc gcaagcgtta ttcggattca ctgggcataa agagcacgta ggcggctacg
       61 taagtcggat gtgaaatccc gcggctcaac cgcggaatgg catccgatac tgcgtggctc
      121 gaggtcggaa ggggtaagcg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcttactggt ccgaatctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002183
VERSION     KHUT01002183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           97 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggttcgt
       61 tatgtctgtg gtgaaagccc tgggctcaac ctaggaatgg ccatggaaac tggcgggctg
      121 gagtatggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002184
VERSION     KHUT01002184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcacac gtgaaatccc caggctcaac ctgggaactg cgtgtgatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgacattga cgctgatgag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002185             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002185
VERSION     KHUT01002185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           48 c          100 g           52 t
ORIGIN      
        1 tacggaggtg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctgt
       61 taagtgaggg gtgaaaggct gaggctcaac ctcatgtttg tcctctcata ctggcagtct
      121 tgagtgaggc agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccttaactg acgctgaggc gcgaaagctg
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002186
VERSION     KHUT01002186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcgag
       61 tgagtggaat gtgaaatgcc ccggctcacc cggggaccgg cattccatac tgctcggctc
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002187
VERSION     KHUT01002187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           85 g           51 t
ORIGIN      
        1 tacgtaccgt acgaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggcctta
       61 aaggtgaggt gtgaaatccc acggcttaac cgtggaactg cgcttcaaac cataaggctt
      121 gagggagata gaggaaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctttctggg tctcttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002188
VERSION     KHUT01002188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcagtg gtgaaatcct gcagcttaac tgcagaagtg cctttgatac tggcgagctc
      121 gagggaggta ggggtatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002189
VERSION     KHUT01002189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggtctcg
       61 caagtcaagt gtgaaagccc cgggcttaac ctgggaactg cgcttgaaac tacgagactc
      121 gagtgtggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccttctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002190
VERSION     KHUT01002190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggattgg
       61 tgtgtcagat gtgaaatccc tgggctcaac ccaggaactg cacctgaaac taccagtctt
      121 gagtggtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gcttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002191
VERSION     KHUT01002191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           91 g           44 t
ORIGIN      
        1 gacgaaggtg gctagcgtta ttcggattca ctgggcataa agcgcgcgta ggcggctctt
       61 caagtcggtg gtgaaagccc gcagctcaac tgcggaacag ccttcgatac tgtcgagctt
      121 gagtgcagga ggggtgatcg gaactccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgga ggcgaaggcg gatcactgga ctgccactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002192
VERSION     KHUT01002192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           89 g           47 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggctagt
       61 caagtctgct gttaaagcgt gcagctcaac tgcataacgg cggtggaaac tgattagcta
      121 gagtatggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg ctctactggg ccaaaactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01002193             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002193
VERSION     KHUT01002193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcatt
       61 taagtttggt gtttaatccc ggagctcaac tccgggtcgc actgaaaact gggtgacttg
      121 agtacagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01002194             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002194
VERSION     KHUT01002194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           98 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaaactc cgggctcaac tcggagacgc cactcgatac tgccatgact
      121 cgagtccggt aggggagtgc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002195
VERSION     KHUT01002195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           87 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg ctgaaatccc ccggcttaac cggggtggtg gcattgatac tgcagggctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactggg ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002196
VERSION     KHUT01002196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           94 g           53 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgatc
       61 ctagtcagat gtgaaagtcc tgggcttaac ctggggactg catttgatac tggattgctt
      121 gaatgcagga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctatctgga ctgtgattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002197
VERSION     KHUT01002197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggttctt
       61 caagtcagag gtgaaagccc ggtgctcaac accggaattg cctttgaaac tagggaactt
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002198
VERSION     KHUT01002198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           97 g           38 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctcag
       61 cgcgtggggt gtgaaaggtg gcggcttaac tgccacacgc catcccatac ggctgggctc
      121 gagccatgca gaggtccacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg catgtgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002199
VERSION     KHUT01002199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           85 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgagcgca ggcggatcag
       61 aaagtcagat gtgaaatctc gaagctcaac ttcgaaactg cgtctgaaac ttctagtcta
      121 gaatgtcgga ggggataggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctatctgga cgactattga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002200
VERSION     KHUT01002200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           88 g           51 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggccttg
       61 taagtcgggt gtgaaatccc acggctcaac cgtggaactg cgcccgatac tgcatggctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002201
VERSION     KHUT01002201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggctgga
       61 atagtccggt gtgaaatcca caggcttaac ctgtgaatgc cgctggaaac tgtctggcta
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002202
VERSION     KHUT01002202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtcaag
       61 taagtggaat gtgaaacgcc tcggcttacc cgaggacctg catcccaaac tgcttggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002203             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002203
VERSION     KHUT01002203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           96 g           46 t
ORIGIN      
        1 cacgtagggg ccaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcag
       61 ttagtcgggt gtgaaatctc cgggctcaac ccggaggggc cacccgatac tgctgtgact
      121 cgagtccggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002204
VERSION     KHUT01002204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgaacgta ggcggctttt
       61 caagtcaggg gtgaaatccc ggagctcaac tccggaactg cccttgaaac tggatcgcta
      121 gaatcctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcgctgga caggtattga cgctgaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002205
VERSION     KHUT01002205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          106 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtgggg
       61 taagcgtgtg gtgaaagtcc ggggctcaac cccggatcgg ccgtgcgaac tgcttcactt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002206
VERSION     KHUT01002206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           90 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggagag
       61 aaagtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac ttcccttctt
      121 gagtgccgga gaggaaagcg gaatacctgg tgtagaggtg aaattcgtag atatcaggtg
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002207
VERSION     KHUT01002207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           85 g           48 t
ORIGIN      
        1 gacgagcctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaacag cgctcaaaac taccgtgctc
      121 gagggagata gaggaatgcg gaacttacgg tggagcggtg aaatgcgttg atatcgtagg
      181 gaacaccggt ggcgaaagcg gcattctgga tctcttctga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002208
VERSION     KHUT01002208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           81 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgtgta ggcggacctt
       61 taagtagaaa gtgaaaggta ggagctcaac tcctacattg ctctctatac tggaggtctt
      121 gagtgtcgga gaggaagatg gaactgcacg tgtagcggtg aaatgcgttg agatgtgcag
      181 gaacaccaat ggcgaaggca atcttctgga cgaaaactga cgctgagaca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01002209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002209
VERSION     KHUT01002209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgtc
       61 cacgtcgggt gtgaaagctc ggggctcaac cctgagaggc cattcgatac gggacggcta
      121 gaggcaggta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01002210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002210
VERSION     KHUT01002210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           87 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggagtta ctgggtgtaa agggcgtgta ggcgggaaga
       61 taagtcagat gtgaaattcc atggctcaac tgtggcgctg catctgaaac tatttttctt
      121 gagtgatgga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga cattaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002211
VERSION     KHUT01002211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           99 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggtt gtgaaagccc ggggcttaac cccgggtctg cagtcgatac gggcaggcta
      121 gagttcggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002212
VERSION     KHUT01002212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 taagtcggat gtgaaatctc agggctcaac cctgaaactg cattcgatac tgccttgctc
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002213
VERSION     KHUT01002213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           99 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtcggcc gtgaaaacct gcagcttaac tgtgggcctg cggtcgatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgaaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002214
VERSION     KHUT01002214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c           99 g           40 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcccgt
       61 taagtcggat gtgaaaacct ggggctcaac cccaggcctg catccgatac tggcgggctc
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002215
VERSION     KHUT01002215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcgggtcgt
       61 taagttgggg gtgaaatccc ggggctcaac ctcggaactg ccttcgatac tggcgacctt
      121 gagtatggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactggc ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002216
VERSION     KHUT01002216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           94 g           52 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttgat
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggtcagctg
      121 gagggaggta ggggcagttg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002217
VERSION     KHUT01002217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaaacgc gaggctcaac ctcgcgcctg cagtgggtac gggcaaacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccactactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002218
VERSION     KHUT01002218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggccccg
       61 ttagtccgct gtgaaagtcc agggctcaac cctggaatgc cggtggatac tgcggggcta
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002219
VERSION     KHUT01002219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggg
       61 caagtccggg gtgaaatcct gcagcttaac tgtagaagtg ccttggatac tgctcggctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcagactgga cctcttctaa cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002220
VERSION     KHUT01002220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           83 g           54 t
ORIGIN      
        1 tacgagggat acaagcgttg tccggaatga ttgggcgtaa agcgtctgta ggtggcttat
       61 gaagtctgct gttaaatacc agggcttaac cttgggaaag cggtggaaac tcttgagctt
      121 gagttcggta ggggcagagg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccaat agcgaaagca ctctgctggg cctgaactga cactgagaga cgaaagctag
      241 gggagcaaaa agg
//
LOCUS       KHUT01002221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002221
VERSION     KHUT01002221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggatgat
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaactg catttgagac tggtcgtcta
      121 gagtacagaa gaggggggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg accccctggt ctgatactga cgctgaggtg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002222
VERSION     KHUT01002222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggcggtctgt
       61 taagtcgttt gtgaaaatcc ggggctcaac tccggaaatg cgtacgatac tggcaggctg
      121 gagactggta gaggctagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacacctgt ggcgaaggcg gctagctggg ccagttctga cgctcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002223
VERSION     KHUT01002223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggacatt
       61 taagtcaggg gtgaaatgcc aaggctcaac cttggaactg cctttgatac tgtatgtctt
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002224
VERSION     KHUT01002224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 ttagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac tgcgagtctc
      121 gagggggata ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002225
VERSION     KHUT01002225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgct
       61 caagtcgggt gtgaaagccc ggggctcaac cccggaactg cactcgagac tgtgtggctg
      121 gagatcggaa gaggagagtg gaacacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaagcg gctctctggt ccgattctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002226
VERSION     KHUT01002226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           90 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggattct
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tgagagtctc
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002227
VERSION     KHUT01002227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggggagg
       61 taagtcagcg gtgaaatccc cgagcttaac ttgggaactg ccattgatac tatctctctt
      121 gaatatcttg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cggtaattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002228
VERSION     KHUT01002228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacgtaggat gctagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcaccc
       61 taagtacttt gtgaaatcca cctgctcaac gggtggggtg cagggtaaac tggggagcta
      121 gagagcggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtatctga cgccgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002229
VERSION     KHUT01002229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 tacggggggg gtaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggatggctt
      121 ggatccggaa gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctggg ccggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002230
VERSION     KHUT01002230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcagg
       61 taagtgggat gtgaaagccc tcggctcacc cgaggatctg catctcaaac tgcctggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002231
VERSION     KHUT01002231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggactta ctgggcgtaa agcgcgtgca ggcggattgg
       61 tatgtgccgt gtgaaagctc ccagcttgcc tgggagaggt cgcggtagac agccgatctg
      121 gaggctcgga gaggtcagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gctgactggc cgagacctga cgctgagacg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002232
VERSION     KHUT01002232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacgtaggat gctagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcactg
       61 taagtaaggt gtgaaatctc tctgctcaac ggggagggtg cactttagac tgcagagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002233
VERSION     KHUT01002233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ttgggcgtaa agcgcgcgca ggcggtcgat
       61 taagtcaggt gtgaaaggct gcggctcaac cgcagagcgg cacctgaaac tggtcgactt
      121 gagtgcagga gaggggagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002234
VERSION     KHUT01002234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaatg
       61 taagtcgggc gtgaaatccc taggctcaac ctaggaactg cactcgaaac tgcgtttctt
      121 gagtatcgga gaggaaagcg gaatacctgg tgtagaggtg aaattcgtag atatcaggtg
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002235
VERSION     KHUT01002235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacggagggg gccagcgttg ctcggaatta ttgggcgtaa agggcgcgta ggcggccagg
       61 taagtttgat gtgaaatccc acggcttaac cgtggaattg catcttatac tgcctggctg
      121 gagtctggga gagggaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccagtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002236
VERSION     KHUT01002236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgttt atcggaatta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaattg catccgatac tggcgagctg
      121 gagtgcgata gaggggggta gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccccctgga tcgacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002237
VERSION     KHUT01002237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctgag
       61 taagtcgatt gtgaaatccc cgggctcaac ctgggaattg cagtcgagac tgctcggcta
      121 gagtgtggga gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accgcctggc ccaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002238
VERSION     KHUT01002238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           53 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgtgg
       61 taagtctggt gtgaaatccc ggggctcaac cccggaactg cactggatac tgccatgctt
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002239
VERSION     KHUT01002239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           87 g           46 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccatg
       61 tgtgtcaggt gtgaaagccc gaggctcaac ctcggaattg cgcctgaaac tacatggcta
      121 gagtactgga gagggtagcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccaga ggcgaaggcg gctacctgga cagttactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01002240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002240
VERSION     KHUT01002240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           94 g           54 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtgtct
       61 taagtttgtt gtgaaatctc ccggcttaac cgggagggtg cggcgaatac tgagatgctg
      121 gagtgcggga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga gatgtagatg gcttactgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002241
VERSION     KHUT01002241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agaatgcgta ggtggatatt
       61 taagtgggat gtgaaatccc cgggctcaac ccgggaactg cattccaaac tggatatctg
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cactgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002242
VERSION     KHUT01002242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           83 g           48 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtcagat gtgaaatccc tcggctcaac cgaggaactg cgtttgaaac taccgtgctt
      121 gagtaagaca gaggtaagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tctttactga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002243
VERSION     KHUT01002243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           93 g           45 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggcactt
       61 caagtctgcg gtcaaagacc ggggcttaac cctggaaagg ccgtggaaac tgaagtgcta
      121 gagtgcagta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agctcgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga ctgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01002244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002244
VERSION     KHUT01002244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           84 g           56 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ttgggcgtaa agggtgcgca ggtggttata
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg cgtttgaaac tatatagcta
      121 gagtgtggca gaggggagtg gaattccatg tgtagcagtg aaatgcgtag agatgtggaa
      181 gaacaccgat ggcgaaggca gctccctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002245
VERSION     KHUT01002245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           87 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtttga
       61 taagtccggg gtgaaagccc acagctcaac tgtggaactg ccctggatac tgtcagactt
      121 gagggtagac gaggttggcg gaatagaagg tgtagcggtg aaatgcatag ataccttcta
      181 gaaccccgat tgcgtaggca gctgactagg ctacacctga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002246
VERSION     KHUT01002246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggcttcc
       61 tacgtcggac gtgaaatccc tcggctcaac cggggaactg cgtccgatac ggggcggctt
      121 gaatccaaga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ttggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002247             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002247
VERSION     KHUT01002247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           64 c           99 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccat
       61 cacgtcggtc gctgaaagcc cccggctcaa ccggggaacg ggcgccgata cgagtggcct
      121 ggaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accccatctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002248
VERSION     KHUT01002248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggttcga
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgtcgagcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002249
VERSION     KHUT01002249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtcagat gtgaaagccc tgggctcaac ctgggaactg catttgaaac tggcggacta
      121 gagtttagta gaggggagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg actccctgga ctaaaactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002250             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002250
VERSION     KHUT01002250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggaaag
       61 caagtctgta agtgaaatcc agccgctcaa cggctgggct gcttcggata ctacttttct
      121 agggaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccgggtccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002251
VERSION     KHUT01002251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgcgt
       61 taagtgtgat gttaaatcgt ggggctcaac cctatcgatg catcgcatac tggcgtgcta
      121 gaggacagca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ctactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002252
VERSION     KHUT01002252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           95 g           41 t
ORIGIN      
        1 gacggagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggccaac
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaatgg ctcctgatac tggttggctc
      121 gaggacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtctctga cgctgaggtg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002253
VERSION     KHUT01002253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 taagtgggat gtgaaatgcc ccggctcacc cggggacctg catcccaaac tgctgagctt
      121 gagtacagga gagggtgggg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg cccacctggc ctgctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002254
VERSION     KHUT01002254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgcgta ggcggttgaa
       61 caagttggat gtgaaagccc tgggctcaac ccaggatgtg cattcaaaac tgttcgactt
      121 gaatctcgga gggggtcaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg ctgacctgga cgatgattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002255
VERSION     KHUT01002255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           88 g           55 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ctgggtgtaa agggtgcgta ggcggtttgt
       61 taagtcagaa gtgaaattta ggggctcaac ctctaagctg cttctgaaac tgatgaacta
      121 gagtgtagga gaggaaagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt agcgaaggcg gctttctgga ctattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002256
VERSION     KHUT01002256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           88 g           49 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtacgta ggcggcgctg
       61 tcagtcgggt gtgaaatccc acggctcaac cgtggaactg cgcccgatac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01002257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002257
VERSION     KHUT01002257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctcg
       61 tgtgtcggat gtgaaatccc atggctcaac cgtggaaggg cattcgaaac tacgaggctg
      121 gagtgctgga gaggaaggtg gaatgctcgg tgtagaggtg aaattcgtag atatcgagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002258
VERSION     KHUT01002258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           46 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgtc
       61 cacgtcgaat gtgaaagctc agagctcaac tctgagaggc cattcgatac gggacggctt
      121 gagtcagcta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg gctgttctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002259
VERSION     KHUT01002259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           87 g           49 t
ORIGIN      
        1 gacgagccac ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 caagtcggga gtgaaatacc tcagctcaac tgaggaactg ctctcgatac tgctgtgctt
      121 gagtgagaca gaggtaagcg gaacatcggg tggagcggtg aaatgcgttg atatccgatg
      181 gaacaccggt ggcgaaggcg gcttactggg tctctactga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002260
VERSION     KHUT01002260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           78 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggactga
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaattg ccattgatac tgttggtctt
      121 gagtacagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaag ctgtaactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01002261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002261
VERSION     KHUT01002261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          103 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggccgga
       61 caagtctgcc gtgaaagccc agggcttagc cttggaaggg cggtagaaac tgtcgggctt
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggtgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002262             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002262
VERSION     KHUT01002262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           93 g           44 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctttg
       61 tcagtcgggt gtgaaacctc caggctcaac ctggagccgc cacccgatac tgcaatagct
      121 agagtccggt aggggagcat ggaactcctg gtgtagcggt gaaatgcgca gatatcagga
      181 agaacaccag tggcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002263             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002263
VERSION     KHUT01002263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           50 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcgggt gtgaaaactc tgggctcaac tcagagacgc cacccgatac tgttgtgact
      121 tgagtccggt aggggagtat ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccag tggcgaaggc ggtactctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01002264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002264
VERSION     KHUT01002264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           92 g           55 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgtgta ggcggttttg
       61 taagtctgat gtgaaagccc tgggctcaac ccgggaagtg cattggatac tgtaagactt
      121 gaatacggga gagggcagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002265             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002265
VERSION     KHUT01002265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           94 g           44 t
ORIGIN      
        1 aacgtaggac gcaaacgtta tccggaatta ctgggcgtaa agggcgcgca ggcggttggg
       61 taagtgaggc atgaaagccc catggctaaa cctggggagg ttgtctgata ctgcccgact
      121 agagtacgat agaggggtgt agaattccgc gtgtagtggt gaaatgcgta gagatgcgga
      181 ggaataccag aggcgaaggc ggcaccctgg atcgtaactg acgctgaggc gcgaaagcat
      241 gggtagcgaa cggg
//
LOCUS       KHUT01002266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002266
VERSION     KHUT01002266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           81 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggtcta
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tataggtctt
      121 gaatattgtg gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag agatgacaca
      181 gaacaccgat tgcgaaggca gcccactacg caactattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002267
VERSION     KHUT01002267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttag
       61 cgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac ggctagacta
      121 gagtagatga gagggtgatg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gtcacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002268
VERSION     KHUT01002268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           77 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggctttt
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cctttgatac tgaagagctt
      121 gaatacacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactatg gtgtcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002269
VERSION     KHUT01002269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           51 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggttcgta ggcggccttg
       61 taagtggggt gtgaaatccc tcggctcaac cgaggaatag cgctccaaac tgcaaggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002270             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002270
VERSION     KHUT01002270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           89 g           47 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatga ttgggcgtaa agcgtccgta ggtggttttt
       61 ccagtcggct gttaaagagc aaagctcaac tttgtaaagg cagtggaaac tgaaagacta
      121 gagagccgta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaagcg ctctactgga cggcatctga cactcacagg acgaaagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01002271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002271
VERSION     KHUT01002271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtggag
       61 aaagtagggt gtgaaagccc tcggcctaac cgaggaattg cgccctaaac tacttcgctg
      121 gaggaggata gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg tcctttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002272
VERSION     KHUT01002272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           98 g           41 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcacac
       61 taagtaagat gtaaaatctc cctgctcaac ggggagggcg catcttagac tggtgagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002273
VERSION     KHUT01002273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           91 g           46 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctctt
       61 caagtctgct gtcaaagagc aaggctcaac cttgtcaagg cagtggaaac tgaaaggcta
      121 gagtgtggta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctactgga cctgcactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01002274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002274
VERSION     KHUT01002274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcctt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tattggtctt
      121 gaattttgtt gaggtaggcg gaataagtca tgtagcggtg aaatgcatag atatgactta
      181 gaacaccaat tgcgaaggca gcttgctaaa caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002275
VERSION     KHUT01002275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctat
       61 caagtccggg gtgaaatcct ccagcttaac tggagaacgg cctcggatac tgatggtctc
      121 gaggaggata ggggcacacg gaactgtggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaagcg gtgtgctgga tcctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002276
VERSION     KHUT01002276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c          100 g           44 t
ORIGIN      
        1 gacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggcgggtgga
       61 taagtggttc gtgaaatatc caggctaaac ctggggagga cgaatcagac tgttcatctg
      121 gaggcgggta gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag atatccggag
      181 gaacaccaga ggtgcagacg gcgccctggg cccgacctga cgctgagacg cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002277             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002277
VERSION     KHUT01002277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcaat
       61 taagttgggt gtttaagccc ggggctcaac cccggttcgc atccaaaact ggttgacttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01002278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002278
VERSION     KHUT01002278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           98 g           47 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgat
       61 cgcgtcggat gtgaaagctc ggagctcaac tctgagaggt cattcgatac gggtcggcta
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgttctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01002279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002279
VERSION     KHUT01002279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccatg
       61 taggtccgat gtgaaaactc gaggctcaac ctcgagctgt cattggaaac catgtggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002280
VERSION     KHUT01002280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           94 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagttcgta ggcggtttgt
       61 cgcgtcgttt gtgaaaaccc ggggctcaac ttcgggcttg caggcgatac gggcagactt
      121 gagtgtttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aaacaactga cgctgaggaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002281
VERSION     KHUT01002281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggttgg
       61 taagtcagtg gtgaaatccc taagcttaac ttgggaactg ccattgatac tattaatctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgaatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002282
VERSION     KHUT01002282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agggcgtgta ggcgggcagg
       61 taagtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac tacttgcctt
      121 gagtgccgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002283
VERSION     KHUT01002283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           97 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggaatgc
       61 tttgtcgggc gtgaaattcc agggcttaac cttgggactg cgttcgagac gggtattcta
      121 gagtggagaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt cttttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002284
VERSION     KHUT01002284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgac
       61 caagtcggag gtgaaatccc tcggctcaac tgaggaactg cctccgatac tgttcggctt
      121 gagtcccgga gagggtagtg gaattccgag tgtagcggtg aaatgcgtag agactcggaa
      181 gaacaccagt ggcgaaggcg gctacctgga cgggtactga cgctgaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002285
VERSION     KHUT01002285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           99 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg atcggagtta ctgggcgtaa agggcgcgta ggcggtcgtg
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagag catcgcaaac gacacgactg
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002286             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002286
VERSION     KHUT01002286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           37 c           77 g           61 t
ORIGIN      
        1 gacataggtc gcaaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggttcag
       61 taagttactg gtgggaaatc gaggctcaac ctcgtggaag tcagtaatac tgctgaactt
      121 gagtgcagga gaggttaacg gaactttatg tggagcggta aaatgcgtag atatatgaaa
      181 gaacatcaat atggcgaagg cagttaacta ttctgttact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01002287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002287
VERSION     KHUT01002287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggag
       61 caagtcaacg gtgaaagctc gacgctcaac gtcgagtcgg ccgtcgagac tgctttgctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002288             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002288
VERSION     KHUT01002288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcactt gtgaaatctc ggggctcaac cccgagcggc caagtgatac tgtcgtgctt
      121 ggagtgcgga aggggcaacc ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002289
VERSION     KHUT01002289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           85 g           53 t
ORIGIN      
        1 tacagagatc tcgagcgtta atcggagtca ctgggcttaa agcgtacgta ggcggacttg
       61 caagcatctt gtgaaatccc tcggctcaac cgaggaactg cttggtgaac tgcgagtctt
      121 gaggcaagta gaggctgtcg gaacgatagg tggagcggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaaaaca ggcagctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggatcaaac ggg
//
LOCUS       KHUT01002290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002290
VERSION     KHUT01002290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttatt
       61 taagtgagat gtgaaagccc tgggcttaac ctaggaactg catcttatac tgggtaactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002291
VERSION     KHUT01002291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           49 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tggttgtctt
      121 gagccactta ggggcagtcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaaa ggtgaaaaca gactgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002292
VERSION     KHUT01002292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           96 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgcgc
       61 taagtcggat gtgaaaactc tgggctcaac ccagagcctg catccgaaac tggcgtgcta
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagaaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002293
VERSION     KHUT01002293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtctgt
       61 taagtcatgt gtgaaatccc tcggctcaac cgaggaagtg cgcatgaaac tgacaagcta
      121 gagtactgaa gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccccctggt cggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002294
VERSION     KHUT01002294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          104 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggtccga
       61 tgcgtcgtgc gtgaaagacc ccggctcaac tggggagggt cgtgcgatac ggtcggactc
      121 gaggccagta gggggacgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcgtcctgga ctggtactga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01002295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002295
VERSION     KHUT01002295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttgtg
       61 caagacagag gtgaaatccc cgggcttaac ctgggaactg cctttgtgac tgcacggcta
      121 gagtgtgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gccccctggg atgacactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002296
VERSION     KHUT01002296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           85 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttct
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgaggtg ccattgatac tggggaactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcttactaaa gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002297
VERSION     KHUT01002297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           82 g           61 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggtggtttgg
       61 taagttagat gtgaaagccc tgggcttaac ccgggaattg catttaagac tgccttactt
      121 gaagatcaca gaggtaagca gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaataccgat ggcgtaggca gcttactggg tgatacttga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002298
VERSION     KHUT01002298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 tacgtaggtc gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggctggc
       61 caagtccgat gtgaaagctt ccggctcaac cgggaaaatg catcggaaac tggtcggctt
      121 gaaggtggga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggc ccattcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002299
VERSION     KHUT01002299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           99 g           49 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggttttc
       61 caagtctggt gtgaaatctc ccggctcaac tgggagggtg cgctagaaac tgggaggctg
      121 gagtgcggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcctgctgga ccgtaactga cgctgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002300
VERSION     KHUT01002300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           54 c           76 g           46 t
ORIGIN      
        1 tacagagacc ccaagcatta tccggaatta ctgggcgtaa agggcaagta gccggttgga
       61 ttagtctgat gtaaaaccat ggggcccaac tccatgtccg catcggaaac ggtacaacta
      121 gagcggatga gaggagagcg gaacgtatgg tgtaggggtg aaatccactg atatcatacg
      181 gaacaccaaa agcgaaggca gctctctagc gtcctgctga cggtcaattg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01002301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002301
VERSION     KHUT01002301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           83 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ctgggcgtaa agcgtacgta ggcggaagct
       61 taagtgtgat gttaaatcgt gtggctcaac catatcgatg catcgcatac tgggtttcta
      121 gaggacggca gaggcaagtg gaactcgtag tgtagcggta aaatgcgtag atattacgaa
      181 gaacaccaat ggcgaaggca gcttgctggg ccatttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002302
VERSION     KHUT01002302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           89 g           45 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctcag
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tgctaagcta
      121 gaatcctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002303
VERSION     KHUT01002303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggccagg
       61 taagtcaggt gtgaaagccc ggggctcaac cccgggactg cacttgaaac tatctggcta
      121 gagtgtcgga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga cgataactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002304
VERSION     KHUT01002304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          106 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgggc
       61 gaagcgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccatgcgaac ttgccggctg
      121 gagcacggta gaggccaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttggctggg ccgttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002305
VERSION     KHUT01002305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           74 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggtggacttt
       61 taagtcagtg gtgaaagcct gtagcttaac tatagaattg ccattgaaac tgaaagtctt
      121 gagtacagtt gaggtgagcg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgcaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002306
VERSION     KHUT01002306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           87 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggccggg
       61 taagtctgat gtgaaatccc gcagcttaac tgcggaactg catcggatac tctccggcta
      121 gaggaatgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctaa cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002307
VERSION     KHUT01002307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           97 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattta ttgggcgtaa agcgcgcgca ggcggccgtg
       61 caagtcggat gtgaaaacac gaggctcaac ctcgtgcctg cattcgatac tgctcggcta
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002308
VERSION     KHUT01002308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          108 g           36 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggttgg
       61 tgagtcgggg gttaaaggcc ggggctcaac cccggaaggt ctcccgatac tgcgagccta
      121 gggaccggta ggggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002309
VERSION     KHUT01002309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           87 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgat
       61 taagtcagcg gtgaaagtct gtggctcaac catagaagcg ccgttgatac tggccggctt
      121 gagtgccgta tgggtacatg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgtaggca ttgtactgtg cggcaactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002310
VERSION     KHUT01002310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           47 c          100 g           54 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgtt
       61 cgagtcgggt gtgaaatcct gaggcttaac cttgggtctg cattcgatac tgggcggctt
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002311
VERSION     KHUT01002311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgat
       61 taagtcagcg gtgaaatccc ggggctcaac cccggaactg ccgttgatac tggttggctt
      121 gagttcggtc gaggcgggcg gaactggtgg tgtagcggtg aaatgcatag ataccaccaa
      181 gaaccccgat tgcgaaggca gctcgctggg ccgaaactga cgctgaggca cgaaagcgcg
      241 gggagcgaac agg
//
LOCUS       KHUT01002312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002312
VERSION     KHUT01002312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           99 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcggt
       61 caagtcactt gtgaaatctc cgggcttaac ccggagcggc caagtgatac tgttcgactg
      121 gagtgcggaa ggggctactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg ggtagctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002313
VERSION     KHUT01002313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggactcg
       61 taagtcagtg gtgaaatctc acagcttaac tgtgaaactg ccattgatac tgcgggtctt
      121 gagtatagtt gaagtagctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggttactaaa ctataactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002314
VERSION     KHUT01002314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           98 g           44 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcgggtcac
       61 caagtccggc gtgaaagctc ccggctcaac tgggagaggg cgttggaaac tggtggtctt
      121 gaatctcaga gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca accggctggc tgaagattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002315
VERSION     KHUT01002315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           99 g           44 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcttg
       61 taagtcagct gtgaaagccc ggggctcaac cccggagggt cggctgatac tgcaggactg
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002316             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002316
VERSION     KHUT01002316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           38 c           79 g           62 t
ORIGIN      
        1 aacggagggg gcaagtgtta ctcgtaagga ctgggcgtaa agggttcgta ggcggtttgt
       61 agtaagttag gtgttaaata gtaagtctca aacttatttt tgcatctaaa actgacaata
      121 ctggagtttg atagaggata agggtacttt tgatgaagag gtagaattcg tagatatcaa
      181 tagggaccac cacctggcga aggcgcttat ctgggtcaaa ctgacgctga ggaacgaaag
      241 cgcggggagc aaacagg
//
LOCUS       KHUT01002317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002317
VERSION     KHUT01002317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgctcgta ggcggatcga
       61 tcagtcaggg gtgaaatccc agggctcaac cctggaactg cctttgatac tgtcgatcta
      121 gagtccggaa gaggtgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002318
VERSION     KHUT01002318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           85 g           54 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtatgt
       61 taagttcgtt gttaaaggct ctggcctaac cggagtattg caatgaaaac tggcatgcta
      121 gagggtgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002319
VERSION     KHUT01002319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           82 g           58 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctaa
       61 taagtcagtg gtgaaatcct gcggctcaac cgtagatgtg ccattgatac tgttaggctt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002320
VERSION     KHUT01002320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          100 g           44 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggatgg
       61 caagtcggtc gtgaaatcct tcggctcaac cgaagaagtg cgtccgatac tgctgtcctt
      121 gagggttgga gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggc caatccctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002321
VERSION     KHUT01002321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttttg
       61 taagtgagat gtgaaagccc tgggcttaac ctaggaaccg catcttaaac tgcaaggctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002322
VERSION     KHUT01002322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcta
       61 caagtcgggt gtgaaatccc tcagcttaac tgaggaactg cgcccgaaac tgtagtgctg
      121 gagtgaggca ggggtgagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcactggg cctttactga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002323
VERSION     KHUT01002323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcccat
       61 taagtctgat gtgaaagccc ggggctcacc cccggaagtg cattggagac tgatgggctc
      121 gagtcccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002324
VERSION     KHUT01002324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctctg
       61 ttggtcgtgg gtgaaatccc ccagcttaac tggggaactg cctacgatac cgtggagctc
      121 gagggaggaa ggggcggtcg gaactgtcgg tggagcggtg aaatgcgttg atatcgacag
      181 gaacgccggt ggcgaaagcg gaccgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002325
VERSION     KHUT01002325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           98 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatga ctgggcgtaa agcgcgcgta ggcggctcca
       61 tcagccggtt gtgaaagccc cgggctcaac ctgggaacgg catccggaac tgtggggcta
      121 gagtgcagga gaggaaggta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002326
VERSION     KHUT01002326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           94 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggcggttgct
       61 taagtctgat gtgaaagccc tgggctcaac ccgggaagtg cattggaaac tgggcaactt
      121 gagtacggga gaggatagtg gaattccgag tgtaggggtg aaatccgtag atattcggag
      181 gaacaccggt ggcgaaggcg gctatctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002327
VERSION     KHUT01002327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggtctgt
       61 taagtttgcg gtgaaatccc ggggctcaac tccggatatg ccgcgaatac tggcggacta
      121 gagaccggta gaggcaagtg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gcttgctggg ccggttctga cactcagccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002328
VERSION     KHUT01002328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttcg
       61 caagtcagag gtgaaagccc ggggctcaac cccggaattg cctttgagac tgcgtcgcta
      121 gaatccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002329
VERSION     KHUT01002329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcgggtcgt
       61 taagttgggg gtgaaatccc gaggctcaac ctcggaactg ccttcaaaac tggcgacctg
      121 gagcaaagaa gaggcgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg actcgctggt cttttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002330             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002330
VERSION     KHUT01002330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           50 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaacc gcttcgcata ctggcagact
      121 ggaggaaggc agaggtaact ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaactccaa tggcgaaggc aggttactgg gccttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002331
VERSION     KHUT01002331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcttgg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgccgggctg
      121 gagtactgga gaggagatcg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccggt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002332
VERSION     KHUT01002332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcgggtcgg
       61 taagtctgct ctgaaagtcc aaggctcaac cttgggatgc gggtggatac tgccgacctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002333
VERSION     KHUT01002333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           95 g           42 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggctgcccct
       61 caagtggtgt atgaaagcac ccggctcaac cgggtcatgg tatgccagac tgaagggcta
      121 gagagcgaga gcgggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcatctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002334
VERSION     KHUT01002334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          105 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggtcagg
       61 cgcgttttgt gtgacagcgg gtggcttaac tacccgagga tgcgaaagac ggcctgactt
      121 gagggctcga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc gagtgactga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002335
VERSION     KHUT01002335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          105 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcccgg
       61 tcagttcgtg gtgaaagcgc ggggctcaac cccgcgtcgg ccatgaatac tgcggggctg
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002336
VERSION     KHUT01002336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           87 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggtatca ctgggcttaa agcgtgcgta ggcggtctgg
       61 taggtggggt gtgaaatccc tcggctcaac cgaggaatag cgctccaaac ccccagactt
      121 gaggaagaca ggggtgagtg gaactgttgg tggagcggtg aaatgcgttg atatcaacag
      181 gaacaccggt ggcgaaagcg actcactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002337
VERSION     KHUT01002337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           89 g           44 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcgcgta ggcggtctcg
       61 tatgtcgatt gtgaaagccc tgggctcaac ctaggaactg cagccgaaac tgcgagactt
      121 gaatgttgga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgcctggc caaacattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002338
VERSION     KHUT01002338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           63 c          110 g           36 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgtgcgca ggcggcgcgt
       61 tctgcccggc gtgaaagccc ccggctcaac cggggaacgt cgtcggggac gggcgcgctt
      121 gagggcggca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccagctggg ccgaccctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002339
VERSION     KHUT01002339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          103 g           38 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgca ggcggttcgg
       61 taagtggcgg gtgaaagcgc ctggctcaac caggcgagtg ccggtcagac tgctggactg
      121 gaatgtcgga gagggacgcg gaattccggg tgtagcggtg aaatgtgcag agatccggag
      181 gaacaccagc gccgaaaggg gcgtcctgga cgataattga cgctgagacg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002340
VERSION     KHUT01002340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           92 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagttcggt gtgaaatctc tcggcttaac tgggagggtg cgccggaaac tggagtgctc
      121 gaacgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccattgttga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002341
VERSION     KHUT01002341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           48 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcgggtttg
       61 tcagtcaggg gtgaaatccc ggagctcaac tccggaactg cccttgatac agcaagcctt
      121 gagtccggaa gaggtaggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcctactggt ccggcactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002342
VERSION     KHUT01002342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           85 g           58 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatatt
       61 taagtgagat gtgaaatacc cgagcttaac ttgggtgctg catttcaaac tggatatcta
      121 gagtgtggga gaggagaatg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg attctctgga ccataactga cgctgaggca tgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002343
VERSION     KHUT01002343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccgat
       61 tcagtcagac gtgaaatccc tcggctcaac cggggaactg cgtctgatac tagttggctt
      121 gaatccggga gagggacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga ccggtattga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002344
VERSION     KHUT01002344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          104 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctga
       61 taagtcgggg gtgaaattct gcggcttaac cgcaggacgg ccttcgagac tgtcgggctt
      121 gagcacggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002345
VERSION     KHUT01002345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 gacgaaggtg gctagcgttg ctcggattta ctgggcataa agcgcgcgta ggtggtcttg
       61 taagcggggg atgaaagccc atggctcacc catggaactg tccttcggac tgcaaggctg
      121 gagggcggga ggggagaatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg attctctggc ccgtccctga cactgaggcg cgagagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002346
VERSION     KHUT01002346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggaggtttag
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacggaaac tgctaggcta
      121 gagtgcggta gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggctactgga ccgtcactga ctctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002347
VERSION     KHUT01002347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttttg
       61 taagtcgggg gtgaaatgct gcggctcaac cgcagcgctg ccttcgagac tgcaagactg
      121 gagcacggta gaggcggatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccaat ggcgaaggca gtccgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002348
VERSION     KHUT01002348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           89 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcccgg
       61 taagtcagtg gtgaaataca gccgctcaac ggttgaggtg ccattgatac tgtcgggctt
      121 gaaagaattg gaggctgccg gaatggatgg tgtagcggtg aaatgcatag atatcatcca
      181 gaacaccgat tgcgaaggca ggtggctacg attgatttga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002349
VERSION     KHUT01002349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           98 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggccaga
       61 taagtccggt gtgaaagccc caggctcaac ctgggaatgc cgccggaaac tgtctggcta
      121 gagtccggaa ggggccagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctggctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002350
VERSION     KHUT01002350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           96 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggcctat
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tgggaggcta
      121 gagtgcggga tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgcagacg gctctctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002351
VERSION     KHUT01002351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccacc
       61 cacgtcggat gtgaaagctc agggcttaac cctgagatgc cattcgatac ggggtggcta
      121 gaggcagcta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga gctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002352
VERSION     KHUT01002352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtccgaa gtgaaagtcc agggctcaac cttgggattg ctttggaaac tggcagactt
      121 gagtgctgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccagt ggcgaaggcg ccttgctgga cagtgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002353             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002353
VERSION     KHUT01002353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           63 c           98 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccag
       61 cacgtcgggc gctgaaatcc cccggctcaa ccggggaacg ggcaccgata cgactggcct
      121 ggaggggagt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg gctcttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002354
VERSION     KHUT01002354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggccgtt
       61 cacgtcggat gtgaaagctc agggctcaac tctgagacgc cattcgatac gggacggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002355
VERSION     KHUT01002355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtctgat gtgaaatctc cgagcttaac tcggaaatgg catcggatac tattctgcta
      121 gagggctgga gaggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacccgt ggcgaaggca agtctctgga cagcacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002356
VERSION     KHUT01002356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           63 c          111 g           35 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agggcacgca ggcggcggca
       61 ctggcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac cgtgccgctt
      121 gagggcagca gggggtggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccacctggg ctgcacctga cgctgaggtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002357
VERSION     KHUT01002357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           89 g           53 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggttaat
       61 caagtctatt gtcaaagtgc gaggctaaac ttcgtaaagg cggtggaaac tggttgacta
      121 gagtttggta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg ctctactggg cctgaactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01002358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002358
VERSION     KHUT01002358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           96 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggctcga
       61 taagtctgct gtgaaagtcc agggctcaac cctggaaggc cggtggaaac tgtcgggcta
      121 gaatccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002359
VERSION     KHUT01002359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatca ctgggcgtaa agcgttcgta ggcggtgtgg
       61 caagtcggat ttgaaatccc ggggctcaac tccggaactg gatccgaaac tgccatgctg
      121 gagcgtgtca gagggtagcg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctacctggg gcattgctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002360
VERSION     KHUT01002360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcagcg
       61 taagtcgatt gtgaaagccc cgggcttaac ctgggaattg cattcgatac tgcgctgctc
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc ccaatactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002361
VERSION     KHUT01002361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           86 g           56 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ttgggcgtaa agcgaacgta ggcggcttct
       61 taagtttggt cttaaagacc ggggctcaac cccgggagtg gactgaatac tgggaggcta
      121 gaggatgtca gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaagcg actttctggg acatttctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01002362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002362
VERSION     KHUT01002362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 caagtcccat gtgaaagccc acggcttaac cgtggaagtg catgggaaac tgcagagctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002363
VERSION     KHUT01002363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           85 g           54 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggatttg
       61 taagtcagag gtgaaatctc acggcttaac cgtgaaactg cctttgatac tgcagatctt
      121 gagtttggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccataactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002364
VERSION     KHUT01002364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c          100 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggtggcttgt
       61 tacgtctgct gtgaaagcgc ccggcttaac cgggacgatg cggtagatac gggcaagctt
      121 gagggtgaca gaggagcgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgcaagcg gcgctctggg tcgagcctga cactgagacg cgaaggcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002365             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002365
VERSION     KHUT01002365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           94 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcagcgact
       61 gcaagtcggg tgtgaaagcc ctgggctcaa cctaggaagt gcactcgaaa ctgcagggct
      121 tgagttctgg agaggaaggc ggaattctcg gtgtagaggt gaaattcgta gatatcgaga
      181 ggaacatcgg tggcgaaggc ggccttctgg acagtgactg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002366
VERSION     KHUT01002366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           87 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcttgt
       61 taagtcagga gtgaaagttt gcggctcaac cgtaaaattg cttttgatac tggcaggcta
      121 gaatcaggat gaggttagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccaat tgcgaaggca gctggctaga cctggattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002367
VERSION     KHUT01002367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctgac
       61 taagcttgat gtgaaatccc cgggctcaac ctgggaactg cattgagaac tggtcagcta
      121 gagtgcggca gagggggttg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gacccctggg tcgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002368
VERSION     KHUT01002368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccgtg
       61 gaagttcgat gtgaaagctc ggagcttaac tccgaaatgg cattgaatac tctacggctc
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002369
VERSION     KHUT01002369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           90 g           47 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggtatga
       61 caagtcagat gtgaaagagg ccggcttaac cggcaagggt catttgaaac tgtcagacta
      121 gagtgcagca gaggatagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actatctggg ctgcaactga cgctgaggcg cgaaggctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01002370             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002370
VERSION     KHUT01002370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtcct
       61 gtaagtctgg agtgaaattt cggagctcaa ctccgaagct gcttcggata ctgcggatct
      121 agagaccggt agaggctggt agaattacag gtgtagcggt ggaatgcgta gatatctgta
      181 agaatacccg tggcgaaggc ggccagctgg gccgggtctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002371             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002371
VERSION     KHUT01002371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           84 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcgggtatt
       61 taagtgtgat gcgaaatcct ggggctcaac cccatggact gtatcgcata ctggatacct
      121 agaggatatg agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gagatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg catatttctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002372
VERSION     KHUT01002372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          100 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 caagtcagtg gtgaaagctc ggcgctcaac gccgagtcgg ccactgatac tgccgcgctt
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002373
VERSION     KHUT01002373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgtc
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg cttggtatac tggatgtctt
      121 gagctatcta ggggcagctg gaacgaacgg tggagcggtg aaatgcgtag atatcgttcg
      181 gaacgccaat ggtgaaaaca ggctgctggg gatacgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002374
VERSION     KHUT01002374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggtcccg
       61 caagtcaggc gtgaaatccc cgggcttaac ctgggaatgg cacttgaaac tacgggactt
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccaat ggcgaaggca gcctcctggg tcaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002375
VERSION     KHUT01002375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgt
       61 caagtacctt gtgaaatccc tcggctcaac cggggaatgg ctgggtatac tgacgggctt
      121 gaggttggta ggggtgcctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcccgt ggcgaaagcg gggcactggg ccacacctga cgctaagacg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002376
VERSION     KHUT01002376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgggagtctt
      121 gggccattca ggggcgactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggtcgctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002377
VERSION     KHUT01002377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           43 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggattca ctgggcttaa agcgtgcgta ggcggatgcg
       61 caggcgtcgt gtgaaagccc tcggctcaac cggggaactg cacggcgaac cgcgcgtctt
      121 gagacgagta ggggcggccg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccaaa ggtgaagaca ggccgctggg ctcgttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002378
VERSION     KHUT01002378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           80 g           60 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggctat
       61 taagtcagcg gtgaaatctt cgggctcaac ccggaaactg ccattgatac tattagtctt
      121 gaatactgtg gaggtatgcg gaatgtgtca tgtagcggtg aaatgcttag agatgacaca
      181 gaacaccgat tgcgaaggca gcgtactaca cagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002379
VERSION     KHUT01002379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtttcg
       61 cgagtccgat gtgaaagccc ggggctcaac cccggaagtg cattggatac tacgaagctt
      121 gagtgctgga gaggaaagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagacactga cgctgatacg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01002380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002380
VERSION     KHUT01002380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcgggtcaa
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaactg cctccgaaac tgctgatctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002381
VERSION     KHUT01002381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttgat
       61 taagtccgtt gttaaagccc ctggctcaac tgggggaggt caatggagac tattcgactt
      121 gagggcaaca gaggcacatg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gtgtgctggg ttgcaactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002382
VERSION     KHUT01002382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           83 g           53 t
ORIGIN      
        1 tacggagagt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcgggagag
       61 taagtcagat gtgaaatact gaagctcaac ttcagtgctg catatgaaac tgcctttctt
      121 gagggttgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacccgt ggcgaaagcg gttttctagt tgatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01002383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002383
VERSION     KHUT01002383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           81 g           62 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactgg
       61 taagtcagtg gtgaaatact gcagcttaac tgtagaattg ccgttgatac tgtcagtctt
      121 gagtatggtt aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactgaa ccataactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002384
VERSION     KHUT01002384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           97 g           48 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggattta ttgggtgtaa agggtgtgta ggcggggcgg
       61 taagtcagag gtgaaaggca gcggctcaac cgctggacag cccctgatac tgccggactt
      121 gagttccgga gaggccattg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacacctgt agcgtaggcg aatggctgga cggtgactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002385
VERSION     KHUT01002385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           98 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagtctagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcagactt
      121 gaggagagta gaggagagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ctctacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002386
VERSION     KHUT01002386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           97 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcgggtttc
       61 caagtcgggg gtgaaattcc agggctcaac cctgggacgg ccttcgatac tggcagcctg
      121 gagatcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002387
VERSION     KHUT01002387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           87 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggataat
       61 taagttaggt gtgaaattcc tgggcttaac ctgggaactg catttaatac tggttgtcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gctttctgga ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002388
VERSION     KHUT01002388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggtttgc
       61 taagtcaggt gtgaaatccc tgggctcaac ctaggaattg catttgatac tggcaggctc
      121 gagtgtggta gaggatagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctatctgga ctaacactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002389
VERSION     KHUT01002389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggctggc
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgagac tggcgagctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgtaactga cactgaggaa cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002390
VERSION     KHUT01002390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           74 g           64 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggacttt
       61 taagtcagtg gtgaaagcct gtagcttaac tatagaattg ccattgaaac tgaaagtctc
      121 gagtgtggtt gaggtaactg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca ggttactgag ccattactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002391
VERSION     KHUT01002391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggtctgt
       61 taagcagggt gtgaaagccc ccggcccaac cggggaattg cgccctgaac tggcaggctt
      121 gagtgggata gaggtgtggg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccaga ggcgaaagcg ccacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002392
VERSION     KHUT01002392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 gacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgcgg
       61 caagtcggaa gtgaaatccc tgggcttaac tcaggggctg ctcccgaaac tgccgtgctt
      121 gagtgcggga gaggccagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctggctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002393
VERSION     KHUT01002393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          101 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggtcgcg
       61 tcagtttggg gtgacagctc ctggcttaac cgggagagga cctcgaagac ggcgtgactt
      121 gagggccaga gagggacatg gaactccggg tggagtggtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tggcacctga cgctcagggg cgaaagcgag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002394
VERSION     KHUT01002394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          103 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 cgcgtccggg gtgaaatcct cccgctcaac gggagaacgg cctcggatac gggcggtctg
      121 gaggaaggta ggggcgagcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gctcgctgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002395
VERSION     KHUT01002395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c          102 g           43 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggattga ctgggcgtaa agggcgtgta ggcggtcggc
       61 caagtctgat gtgaaagccc gaggctcagc ctcggaagtg cgtcggaaac tagtcggctg
      121 gagcgcgata gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccctctgga tcgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002396
VERSION     KHUT01002396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taagctagat gtgaaagccc agggctcaac cttggaactg catttagaac tggcaggcta
      121 gagtacagca gaggggtgtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg acactctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002397             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002397
VERSION     KHUT01002397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           49 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 taagtcagat gtgaaaactc agggctcaac ccggagacgc catctgatac tgcggtgact
      121 ggagtctggt aggggaacac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgttctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002398
VERSION     KHUT01002398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           78 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttaa
       61 taagtcagtg tttaaagtct gcagcttaac tgtaggtatg gcattgatac tgttaaactt
      121 gagtaaagtt gaggtgggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gctcactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002399
VERSION     KHUT01002399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           88 g           54 t
ORIGIN      
        1 tacggagggt gccagcgttg tccggattca ttgggtttaa agggtgcgca ggcggaagct
       61 taagtcgggg gtgaaagacg gttgctcaac aatcgcagtg ccttcgatac tgggtttctt
      121 gggtgaagtc gaggcggatg gaatgcaagg tgtagcggtg aaatgcatag ataccttgca
      181 gaactccgat tgcgaaggca gtccgctagg ctttaaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002400
VERSION     KHUT01002400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 caagtttggt gtgaaatccc ggggctcaac cccggaactg cactgaatac tgccttgctt
      121 gagcactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagttgctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002401
VERSION     KHUT01002401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcttg
       61 tatgtctgct gtaaaagccc acggctcaac cgtggagggt cggtagaaac tgcgagactt
      121 gaggctggga gaggctggtg gaattccctg tgtagcggtg aaatgcgtag atatagggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002402
VERSION     KHUT01002402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           99 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcctg
       61 atcgtcggat gtgaaaggcc tgggctcaac ctgggaatgg catccgatac ggcgggactg
      121 gagtgtggga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002403
VERSION     KHUT01002403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtccgt
       61 taagttggta gtgaaatccc atggctcaac catggaagtg ctgccaaaac tggcgggctg
      121 gagtgccgga gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggtaactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002404
VERSION     KHUT01002404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           99 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggttgtg
       61 taagtgggac gtgaaagctt ccggctaaac tggaagagga cgactcagac tgcagaacta
      121 gagtggatca gaggtgcgcg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggggcaaccg gcgcactggg atttgactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002405
VERSION     KHUT01002405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctcat gtgaaatctc caagctcaac ttggaaatgg catgggatac tattcggcta
      121 gaggattgga ggggggactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002406
VERSION     KHUT01002406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          101 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 tcagtcggat gtgaaagccc ggggctcaac cccggaacgg catccgagac gggagggctc
      121 gagaccggga gaggagggtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002407
VERSION     KHUT01002407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcggggaag
       61 caagtcagat gtgaaattcc gaggctcaac ctcggcgctg catctgaaac tgtgtctctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002408
VERSION     KHUT01002408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccaat
       61 taagtcagtg tttaaagtca acagcttaac tgttggaatg gcattgatac tgaatggctt
      121 gagtaaagtt gaggtgggcg gaattgaaag tgtagcggtg aaatgcatag atattttcaa
      181 gaacaccaat tgcgaaggca gctcactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002409
VERSION     KHUT01002409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           49 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccat
       61 taagtcggga gtgaaatctc agggctcaac cctgaaaccg ctctcgatac tggtggactt
      121 gaggcaggta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctgtcctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002410
VERSION     KHUT01002410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtttc
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaagtg ccgtggatac tggaagtctc
      121 gaggagggta ggggcatctg gaactgccgg tggagcggtg aaatgcgttg atatcggcag
      181 gaactccgat ggcgaaggca aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002411
VERSION     KHUT01002411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggtttgg
       61 taggtctgct gtgaaaactc gaggctcaac ctcgagacgc cggcggaaac ccccaagcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002412
VERSION     KHUT01002412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          101 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 caagtccggg gtgaaagccc ccagctcaac tggggaaggg ccttggaaac tgccggtctc
      121 gagggaggta ggggcctgtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg acgggctgga tctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002413
VERSION     KHUT01002413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgca ggcggtttgt
       61 taagtcatgt gtaaaagccc ccggctcaac cgaggaagga cgcatgaaac tggcaagcta
      121 gagtaccaaa gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gccccctggt tggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002414
VERSION     KHUT01002414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcttac
       61 taagacaggt gtgaaagccc cgagcttaac ttgggaatgg cgcttgtgac tggtaggctg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccagcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002415
VERSION     KHUT01002415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctgct
       61 taagtctgac ctgaaagtgg gccgcttaac ggcacagggt ggttggatac tgggcagctt
      121 gaagagggtg gaggccgccg gaacggatcg tgtagcggtg aaatgcatag agatgatcca
      181 gaaccccgat tgcgtaggca ggcggctacg ccccacttga cactgaggca cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002416
VERSION     KHUT01002416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           84 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggaaaat
       61 taagtcaggg gtgaaagact atagcttaac tatggcagtg cctttgatac tgattttctt
      121 gagtacagat gaggctggcg gaatgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gccggctaaa ctgctactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01002417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002417
VERSION     KHUT01002417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgat
       61 cgcgtcggat gtgaaagctc aaggctcaac cttgagaggc cattcgatac ggatcggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002418
VERSION     KHUT01002418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           95 g           41 t
ORIGIN      
        1 gacggagggc gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggatgg
       61 taagtcagac gtgaaatccc ggggctcaac tccgggcctg cgtttgaaac tgccgtccta
      121 gagtgcagga gaggaaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002419
VERSION     KHUT01002419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           86 g           54 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcgg
       61 aaagtcggtt gtgaaatacc tcagcttaac tgaggaactg cagtcgatac tgccgtgcta
      121 gaggaagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gcttactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002420
VERSION     KHUT01002420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           83 g           48 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtgg
       61 aaagttgggt gtgaaatccc tcagcttaac tgaggaacag cgcccaaaac taccatgctc
      121 gagggagata gaggaatgcg gaacttaggg tggagcggtg aaatgcgttg atatcctagg
      181 gaacaccggt ggcgaaagcg gcattctgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002421
VERSION     KHUT01002421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           62 c          107 g           34 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 caagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgccgcgctc
      121 gagcagagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctctggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002422
VERSION     KHUT01002422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggtgtaa agggcgtgta ggcggggatg
       61 caagtcagat gtgaaattcc ggggctcaac ctcggcactg catctgaaac tgtgtctctt
      121 gagtgctgga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg actttctgga cagcaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002423
VERSION     KHUT01002423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           82 g           64 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcatg
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgtatgactt
      121 gagtaaagtt gaggttggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat tgcgaaggca gctaactaag ctttaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002424
VERSION     KHUT01002424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggtttcg
       61 taagtcgggt gtgaaatccc cgggctcaac ctgggaattg cattcgaaac tgcgaggcta
      121 gggtacggaa gagggaagcg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg gcttcctggt ccagtaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002425
VERSION     KHUT01002425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgtgta ggcgggaagg
       61 taagtctgat gtgaaagccc cgggcttaac ctgggaagtg cattggaaac tgcttttctt
      121 gagtactgga gaggaaggga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cccttctgga cagttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002426             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002426
VERSION     KHUT01002426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgca ggcggatcgt
       61 taagtcaggg gtgaaatgcc aaggctcaac cttggaactg cctctgatac tggcgatctt
      121 cgagtccggg agaggtgagt ggaactccga gtgtagaggt gaaattcgta gatattcgga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctcaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002427
VERSION     KHUT01002427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcataa agggcgcgta ggcggctttc
       61 taagtcaggg gtgaaatccg gcagctcaac tgtcgcagtg cctttgatac tggagagctc
      121 gagggaggta ggggtctgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002428
VERSION     KHUT01002428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          100 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtccgct gtgaaaactc ggggcttaac tccgggcctg cagtgggtac gggcagacta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002429
VERSION     KHUT01002429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           98 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagcgtgtg gtgaaagtcc ggggctcaac cccggatctg ccatgcgaac tgccgagctc
      121 gagcattgta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcctgctggg caattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002430
VERSION     KHUT01002430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           90 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgta ggcgggagag
       61 taagtccgcg gtgaaatctc acggctcaac cgtgaaactg ccgttgatac tgcttttctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccgaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002431
VERSION     KHUT01002431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           83 g           50 t
ORIGIN      
        1 gacgaacctt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatacc tcggcttaac cgaggaattg cgcccaaaac tactgtgctc
      121 gaggaagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tcttatctga cgctgaggca cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002432
VERSION     KHUT01002432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           86 g           58 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctcag
       61 taagtcagtg ctgaaatacc ccggcttaac cgggggggtg gcattgatac tgttgagctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaag ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002433
VERSION     KHUT01002433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          102 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgtt
       61 cgcgtcggat gtgaaagctc ggagctcaac tctgagaggc cattcgatac gggacggcta
      121 gaggcaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002434
VERSION     KHUT01002434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 cacgtaggag gcgagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggctcgg
       61 taagtcggtt gtgaaatccc ggggctcaac cccgggactg catccgatac tgccgagcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002435
VERSION     KHUT01002435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcacgca ggcggtcttg
       61 taagttttga gtgacaggtg ggagcttaac tcccgcaggc cttggaaaac tgcaagactt
      121 gagggcttca gagggacgcg gaattccagg tggagcggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gcgtcctggg aagcatctga cgctcatgtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002436
VERSION     KHUT01002436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           84 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagtct gcggctcaac cgcaggagtg ccattgatac tggtagtctt
      121 gagtacagta gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat agcgtaggca gcttactata ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01002437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002437
VERSION     KHUT01002437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          100 g           39 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacgg
       61 caagtcggga gtgaaattcc ggggctcaac tccggaactg ctttcgaaac tgccgacctg
      121 gggaccggta ggggctggta gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002438
VERSION     KHUT01002438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          103 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagcgtgtg gtgaaagcct ggggctcaac cccgggtcgg ccatgcgaac tgccgagctt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002439
VERSION     KHUT01002439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           95 g           43 t
ORIGIN      
        1 aacgtaggac gcaaacgtta tccggaatta ctgggcgtaa agagcgtgta ggcgggtgag
       61 taagttgtcc gtgaaagctc ccggctaaac tgggagaggt cggataagac tgctcgccta
      121 gagggtgtta gagggatgcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcatcctggg acatacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002440
VERSION     KHUT01002440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           93 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctgag
       61 caagtccgct ctgaaagccc gaggctcaac ctcgggaggc gagtggatac tgctcagctc
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gcactctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002441
VERSION     KHUT01002441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccgat
       61 taagtctggt gtgaaagccc ggggctcacc cccggaagtg cgctggaaac tggtcggcta
      121 gagtatggga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctttctgga ccaatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002442
VERSION     KHUT01002442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          102 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggttgtg
       61 tttgtccgct gtgaaagtcc acggctcacc cgtggagggt cggtggaaac ggcacaactt
      121 gaggctggga ggggttggtg gaattccctg tgtagcggtg aaatgcgtag atacggggga
      181 gaacactcgt ggcgaaggcg gccagctggc ccagtcctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002443
VERSION     KHUT01002443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           89 g           54 t
ORIGIN      
        1 tacgaatcgt cctaacgttg ttcggattca ctgggtttaa agggtgcgta ggcggtgcgt
       61 taagtcggtg gtgaaatact ttggctcaac cagagaattg ccgctgaaac tagcgtgctc
      121 gagggaggta gaggtgcatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacacctgt ggcgaaagcg gtgcactgga cctcttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002444
VERSION     KHUT01002444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          101 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcactt
       61 caagtaaggt gtgaaatctc cctgctcaac ggggagggtg cactttagac tgaggagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002445             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002445
VERSION     KHUT01002445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          101 g           47 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctggt
       61 cgcgtcgact gtgaaaaccc gcggctcaac cgtgtggtct gcagtcgata cgggctggct
      121 ggagttcggt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002446
VERSION     KHUT01002446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggcttgt
       61 tcagtcagat gtgaaagccc tgggcttaac ctgggaactg catttgatac tggcaggcta
      121 gagtttagta gaggggagtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacaccagt ggcgaaggcg gctccctgga ctaaaactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002447
VERSION     KHUT01002447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           80 g           59 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattga
       61 taagtcagtg gtgaaaacct gcagcttaac tgtagaattg ccgttgatac tgtcagtctt
      121 gagtacggtc aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gcttactggg ccacaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002448
VERSION     KHUT01002448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           89 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctgtg
       61 taagtctgct gtcaaagaac gaggctcaac cttgtcaggg cagtggaaac tacacggcta
      121 gagtgcgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgcaactga cactcatgga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01002449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002449
VERSION     KHUT01002449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattta ttgggcgtaa agggagcgta ggcggtcggg
       61 taagtcggat gtgaaatcct ggggctcaac ctcagaactg cattcgatac tgcttggcta
      121 gaggaatgga ggggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga catttcctga cgctgatgct cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002450
VERSION     KHUT01002450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtctct
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaaaac tgggaggctt
      121 gaggcaagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctagc cttagcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002451
VERSION     KHUT01002451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 taagtcagtt gtgaaatccc tcggctcaac cgaggaaatg cgtctgagac tgccatgctt
      121 gagtcccgga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga cgggcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002452
VERSION     KHUT01002452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggccttc
       61 taagttcggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tggcaggcta
      121 gagtgtggga ggggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctttctgga ccataactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002453
VERSION     KHUT01002453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           79 g           63 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaacga
       61 taagtcagtg gtgaaatatt gcagcttaac tgtaaaaatg ccgttgatac tgttgttctt
      121 gagtacagtt gaggtgggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgtaggca gctcgctaaa ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002454
VERSION     KHUT01002454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           79 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattgg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tatcaatctt
      121 gaatactgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcggaggca gcttgctaca cagtcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002455
VERSION     KHUT01002455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggatatg
       61 taagttgggg gtgaaatccc ggggcttaac cccggaattg ccctcaaaac tatgtgtctt
      121 gaggccgaga gaggtaaggg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg ccttactggc tcggtactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002456
VERSION     KHUT01002456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           83 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgca ggcggattga
       61 caagtcagag gtgaaatcct gcagcttaac tgcagaactg cctttgaaac tgtcaatctt
      121 gagtacggaa gagagagacg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt tgcgaaggcg gtctcttggt ccgttactga cgctcaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002457
VERSION     KHUT01002457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           95 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgttc
       61 caagtctgtt gtgaaatctc ccggcttaac tgggagggtg cggcggaaac tggaatgctg
      121 gagtgtggga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagacg gcttactgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002458
VERSION     KHUT01002458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c           96 g           52 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgaat gtgaaatttt agggctcaac cctgacattg cattcgatac gggcaggctt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002459
VERSION     KHUT01002459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggtttga
       61 tccgtccgat gttaaagccc ccggctcaac tgggggaggt cattggagac tatcagactt
      121 gagggcagca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg acgtgctggg atgcccctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002460
VERSION     KHUT01002460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           88 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggctgctgcg
       61 caagtcggat gtgaaatccc acggctcaac catggaagtg cattcgaaac tgcgtagcta
      121 gagttttgga gaggaaggcg gaatactcgg tgtagaggtg aaattcgtag atatcgagtg
      181 gaacaccggt ggcgaaggcg gccttctgga caatgactga cgctgaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002461
VERSION     KHUT01002461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccgtg
       61 taagtcactt gtgaaatccc ggggctcaac tccggggctg caggtgagac tgcgtggcta
      121 gagcacggta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccgttgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002462
VERSION     KHUT01002462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           90 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcaagca ggcggctcat
       61 taagtttgac gtgaaatctc ccggcttaac cgggagaagc cgttgaaaac tgataagctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg acaccctggc atttgcctga cgctgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002463
VERSION     KHUT01002463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggtaga
       61 taagtcgggc gtgaaatccc taggctcaac ctaggaactg cactcgaaac tgtttatctt
      121 gagtgccgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002464
VERSION     KHUT01002464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggctag
       61 taagtccgtg gtgaaatccc cgagcttaac tcgggaactg ccgtggatac tattagtctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcggaggca gcttactacg cgattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002465
VERSION     KHUT01002465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggatatg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccgttgatac tatatatctt
      121 gaatgccgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gcttactaca cggagattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002466
VERSION     KHUT01002466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 tatgtccgtt gtgaaagccc cgggctcaac ctgggaatgg caatggaaac tggcgagcta
      121 gagtgtggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002467
VERSION     KHUT01002467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          105 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggtggtggga
       61 caagtctgct gtgaaagcgc ctggcttaac cgggcgaggt cggcggaaac tgtccgacta
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccggccctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002468
VERSION     KHUT01002468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          105 g           42 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggaatta ctgggcgtaa agcgtgtgca ggcggctcat
       61 tgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggtgagctt
      121 gagggtatca ggggcaggta gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaatacccgt ggcgaaggcg gcctgctggg atacacctga cgctgagaca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002469
VERSION     KHUT01002469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           98 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtctgct gtgaaagacc ggggcttaac tccggttctg cagtggatac gggcatgcta
      121 gaggtaggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccttacctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002470
VERSION     KHUT01002470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          100 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacac
       61 taagtacgat gtgaaatctc cctgctcaac ggggagggtg catagtaaac tggtgagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002471
VERSION     KHUT01002471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgccg
       61 caagtccatg gtgaaagctc gatgctcaac atcgagtcgg ccacggagac tgcggggctc
      121 gagcactgta ggggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002472             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002472
VERSION     KHUT01002472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           62 c          112 g           33 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggccgtg
       61 gcaagcgtgc ggtgaaagcc cggggctcaa ccccgggtcg gccgtgcgga ctgccgggct
      121 ggggcccact aggggcaggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcctgctgg ggtggagccg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002473
VERSION     KHUT01002473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           93 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcaggc gtgaaatcca tgggcttaac ccatgaattg cgtttgaaac tggagggctt
      121 gaggatcgga gagggcagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cgacacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002474
VERSION     KHUT01002474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttag
       61 taagtcagat gtgaaagccc tgggcttaac ctgggaactg catttgaaac tgctgaacta
      121 gagtgtggta gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg acttcctgga ccaacactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002475
VERSION     KHUT01002475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttctt
       61 taagtccggg gtgaaagccc actgctcaac agtggaactg ccctggaaac tggcgaactt
      121 gagtacagac gagggtggcg gaatggatac tgtagcggtg aaatgcatag atagtatcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002476
VERSION     KHUT01002476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtacat
       61 taagtttgtt gttaaagact ctggcctaac cggagggttg caatgaaaac tggtgagcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002477
VERSION     KHUT01002477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           99 g           38 t
ORIGIN      
        1 aacgtaggag acaagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggcgcat
       61 taagtctgtt gtgaaagcgc ctggcttaac cgggcgaggc caatggagac tgatgggcta
      121 gaggacggcg gaggggtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcaccctaag ccgtacctga cgctgagacg cgacagcacg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002478
VERSION     KHUT01002478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatca ttgggcgtaa agcgcttgta ggcggcttgt
       61 taagtcgcgt gtgaaatccc ttggctcaac caaggaagtg cgcgcgaaac tggcgagctc
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002479
VERSION     KHUT01002479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           87 g           51 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgatttaa
       61 taagtcttgt gtgaaagccc aaagcctaac tttggaagtg cataagatac tgttgaaatt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002480
VERSION     KHUT01002480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           82 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttta
       61 taagtcggtg gttaaaggca gcagcttaac tgtatgcatg ccatcgatac tgtagagctt
      121 gagtgatttg gaggtagcta gaattccctg tgtagcggtg aaatgcatag atacggggag
      181 gaataccgat tgcgaaggca tgttactacg aattaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002481
VERSION     KHUT01002481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcccgt
       61 taagtccggg gtgaaagcct cttgctcaac aagagaactg ccctggatac tggcgggctt
      121 gagtccagac gaggttggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002482
VERSION     KHUT01002482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgag
       61 caggtccgtt gtgaaaactg gaggctcaac ctccagctgt cgacggaaac cgcacggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002483
VERSION     KHUT01002483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           85 g           49 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggtatca ctgggcttaa agagttcgta ggcggccaag
       61 caggtcaggt gtgaaatccc acagctcaac tgtggaactg cgcttgaaac cgcatggctt
      121 gagggagata gaggtgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002484
VERSION     KHUT01002484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agggcgtgca ggtggtttgc
       61 taagtggtgc gtgaaagcgc ccggctcaac cgggcgaggc cgtgccaaac tggcagactg
      121 gaaacaggca gaggaaagtg gaattcgggg tgtagtggtg aaatgcgtag agatcccgag
      181 gaactccagt ggcgaaggcg actttctggg cctgctttga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002485
VERSION     KHUT01002485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           85 g           56 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgta ggcggaatca
       61 taagtcagag gtgaaatcct gcagcttaac tgcagaactg cctttgatac tgtgattctt
      121 gagtgcggaa gagagagacg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt tgcgaaggcg gtctcttggt ccgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002486
VERSION     KHUT01002486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgtta tccggattta ctgggtgtaa agggtgcgta ggcggcttgg
       61 taagtctaaa gtgaaatttc ggggctcaac cccggagctg ctttggaaac tgccgagcta
      121 gagtgtggga gaggaaagcg gaactttgag tgtagcggtg aaatgcgtag agattcaaag
      181 gaacaccagt agcgaaggcg gctttctgga ccataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002487
VERSION     KHUT01002487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           80 g           59 t
ORIGIN      
        1 tacgtagggg gcaaacgttg tccggattta ctgggtgtaa agggtgctca ggcggttttg
       61 taagtcagaa gtgaaatcct agagcttaac tctggaactg cttttgatac tgcaaagctt
      121 gaatgtggaa gaggaggatg gaatttctgg tgtagcagtg aaatgcgtag atatcagaaa
      181 gaacaccagt ggcgaaggcg gtcctctggt ccactattga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002488             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002488
VERSION     KHUT01002488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcgacg gtgaaatctc cacgctcaac gtggaggggt ccgttgatac tgtcgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgtgactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002489             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002489
VERSION     KHUT01002489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           81 g           58 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtacgta ggtggatagt
       61 ttagtgtgat gcgaaatcct gtggctcaac catatggact gtatcgcata ctggctatct
      121 agaggatatg agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gatatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg catatttctg acgctaaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002490
VERSION     KHUT01002490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttgg
       61 caagtccgtt gtgaaaactg gaggctcaac cttcagacgt cgatggaaac tgcctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002491
VERSION     KHUT01002491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           85 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctcga
       61 taagtcagtg gtgaaagccc atagctcaac tatggaactg ccattgatac tgtcgggctt
      121 gagtacagac gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag ataccatcaa
      181 gaacaccgat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002492
VERSION     KHUT01002492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           97 g           39 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtacctt gtgaaatccc ccggctcaac cggggaacgg ctgggtatac tggcggcctt
      121 gagaggccta ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggccatctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002493
VERSION     KHUT01002493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtttgag gtgaaagctc cgggctcaac ccggaaatgg cctcgaatac tatctggctt
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002494
VERSION     KHUT01002494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           84 g           54 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgt
       61 taagtttgtt gttaaagcct ctagcttaac tggagatcag caataaaaac tggcaagcta
      121 gagtgtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggct gcttcttggc tcataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002495             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002495
VERSION     KHUT01002495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           90 g           45 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtgggtcgt
       61 taagtgtccg cgtgaaatcc cacggctcaa ccgtggaact gccgggcata ctggcgatct
      121 tgaggcattc aggggcgagc ggaacaagtg gtggagcggt gaaatgcgta gatatcactt
      181 ggaacgccaa tggtgaaaac agctcgctgg gaatgatctg acactgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002496
VERSION     KHUT01002496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           99 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtaa ctgggcgtaa agggcgcgta ggcggtgatg
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gacatgactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002497
VERSION     KHUT01002497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           52 c           88 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacctg
       61 taagttggac gtgaaagccc tgggctcaac ccaggaattg catccaaaac tacaggccta
      121 gagtgatgga gagggcagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctgcctgga catcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002498
VERSION     KHUT01002498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           85 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatatt
       61 taagtcagat gtgaaatacc cgagcttaac ttgggtgctg catttgaaac tggatatcta
      121 gagtgtggga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actttctgga ccataactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002499
VERSION     KHUT01002499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           96 g           46 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcttgt
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaatag catttgggac tgatgggcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002500
VERSION     KHUT01002500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggttcgc
       61 taagtcccat gtgaaagccc tcggctcaac tgaggaatgg catgggaaac tggtgggcta
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002501
VERSION     KHUT01002501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggccgag
       61 taagtcgaat gtgaaagccc ccggctcaac tggggaaggt cattcgatac tgttcggctc
      121 gaaggtagga gagggaagcg gaatttcagg tgtagtggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg gctttctggc ctattcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002502
VERSION     KHUT01002502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           66 c          107 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 cgcgtccggg gtgaaatccc cccgctcaac ggggggacgg cctcggatac gggcggcctg
      121 gaggcgggta ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcgtgctgga cccgtcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002503
VERSION     KHUT01002503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          101 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcggtt
       61 caagtcggat gtgaaagccc ggggctcaac cccggaggtg catccgagac tgatctgctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002504
VERSION     KHUT01002504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           75 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcagc
       61 taagtcagtg gtgaaagtct gtggctcaac catagaattg ccattgatac tggttgtctt
      121 gaattactat gaagtggtta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca gatcactaat agtacattga cgctgaggga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002505
VERSION     KHUT01002505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           85 g           52 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agggcgtgca ggcggcctat
       61 caagtcagat gtgaaataac tcggctaaac cgagtggggt catttgaaac tgataagcta
      121 gagtgatgta gaggatagta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg actatctgga cataaactga cgctgaggcg cgaaggctaa
      241 gggagcaaac agg
//
LOCUS       KHUT01002506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002506
VERSION     KHUT01002506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtagac
       61 caagtcactt gtgaaatctc cgggctcaac tcggaacggt caagtgatac tgttcaacta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002507
VERSION     KHUT01002507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tcccgtgctt
      121 gagtgctgga gaggagttcg gaattcacgg tggagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagt ggcgaaggcg gaactctgga cagtcactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002508
VERSION     KHUT01002508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccga
       61 taagtaggag gtgaaattcc gcggctcaac cgcggggcgg ccttctagac tgtcgggctt
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002509
VERSION     KHUT01002509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           60 c           89 g           41 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ctcggaatca ctgggcttaa agggtgcgta ggccgcgcgg
       61 agggtgggat gtgaaatccc ccggctcaac cggggaatag cgtcccaaac cgccgtgctc
      121 gagggagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gctcactggg tctttcctaa cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002510
VERSION     KHUT01002510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           96 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctgc
       61 taagtccggt gtgaaatccc cccgcttaac gggggaacgg cgccggaaac tgacagacta
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002511
VERSION     KHUT01002511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           84 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggtcgtg
       61 tgtgtcaggt gtgaaatccc ggagcttaac tccggaactg cgcctgaaac tacacgacta
      121 gagcattgga gagggaagtg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccaga ggcgaaggcg acttcctgga caattgctga cgctcaggca cgaaagcatg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01002512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002512
VERSION     KHUT01002512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           77 g           65 t
ORIGIN      
        1 tacggaggat tcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggataat
       61 taagtcagag gtgaaagccg gcagctcaac tgtcgaattg cctttgaaac tggttatctt
      121 gaatatagtt gaggtaggcg gaatgtgaca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccgat tgcgaaggca gcttactaaa ctattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002513
VERSION     KHUT01002513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 tacagagggg gccagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccatg
       61 cgtgtcttcc gtgaaatccc tcggctcaac cgaggacgtg cggaagaaac tgcaaggctc
      121 gagtccagaa gaggggggcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccccctggg ctggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002514
VERSION     KHUT01002514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 taagttggat gtgaaaaccc tcggctcaac caaggaagtg cattcaaaac tgcaaggctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002515
VERSION     KHUT01002515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgttcgg
       61 aaagtcgggt gtgaaagccc tgggctcaac ctaggaagtg cactcgaaac tcccgaactg
      121 gagtcccgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacatcggt ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002516
VERSION     KHUT01002516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacat
       61 taagtaaggt gtgaaatatc ccggctcaac cgggagggtg cactttagac tgatgagctt
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002517
VERSION     KHUT01002517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           90 g           43 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgcc
       61 taagtacctt gtgaaatccc acggctcacc cgtggaaccg ctgggtatac tgggcgtctt
      121 gggccattca ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca ggttgctggg aatgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002518
VERSION     KHUT01002518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           99 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggcgtg
       61 taagtcagaa gtgaaattcc ggggctcaac cccggagctg ctcctgatac tgcacgtctg
      121 gagaccggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccagctggg ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002519
VERSION     KHUT01002519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggtggtttgt
       61 aaagtcggat gtgaaagtcc agggctcaac cctgggatgc cattcgatac ttgcaaacta
      121 gagcatggta gggggatctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccattgctga cactgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002520
VERSION     KHUT01002520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           98 g           47 t
ORIGIN      
        1 tacgaggggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgggg
       61 taagtcacct gtgaaatctc ctggctcaac tgggagcttg caggcgaaac tgccttgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002521
VERSION     KHUT01002521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtccga
       61 taagttcgct gtgaaagtcg ggggcttaac cctcggatgc cggtgaatac tgtcgggctt
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002522             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002522
VERSION     KHUT01002522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggat actgaaagcc cccggctcaa ccggggaagt ggttccgaaa cgacctgtct
      121 ggagggacgt agggggatct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctgctg acgctgagac gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01002523             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002523
VERSION     KHUT01002523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           91 g           56 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattta ttgggcttaa agagtgcgta ggtggcagtg
       61 taagtcgggg gtgaaatact atcggctcaa ccgtggaatt gccttcgata ctgcattgct
      121 tgagggggat aggggcaagt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccag tggcgaaagc gacttgctgg gtcttctctg acactgaggc acgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01002524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002524
VERSION     KHUT01002524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           88 g           48 t
ORIGIN      
        1 tacagacggt ccaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggtcagg
       61 aaagtggggt gtgaaagccc tcggcttaac cgaggaattg cgctccaaac tccctgactt
      121 gaggaggcta gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gcttactggg gcctttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002525
VERSION     KHUT01002525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           85 g           54 t
ORIGIN      
        1 gacgagcatc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 aaagtcggga gtgaaatccc acagctcaac tgtggaattg ctttcgatac tgctgtgctt
      121 gagtgagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gcttactggg tctctactga cgctgatgca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002526
VERSION     KHUT01002526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggcgctt
       61 caagtttgat gtgaaagctt cgggctcaac ccggaaatgg cattgaatac tggagtgctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002527
VERSION     KHUT01002527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           93 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtctgc
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tggcgggcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002528             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002528
VERSION     KHUT01002528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcgggt gtgaaaactt caggctcaac ctggagcggc cactcgatac tgccatggct
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002529
VERSION     KHUT01002529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcga
       61 caagtcagtg gtgaaagctc gatgcttaac atcgagtcgg ccactgatac tgttgggctt
      121 gagcactgta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002530             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002530
VERSION     KHUT01002530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           91 g           44 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcgcc
       61 taagtacctt gtgaaatccc ccggctcaac cggggaacgg cttggtatac tgggcgtctt
      121 gagccaacta ggggctgccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaac ggtgaaaaca ggcagctggg gttgtcgctg acgctgagac acgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002531             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002531
VERSION     KHUT01002531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           43 c           77 g           54 t
ORIGIN      
        1 tacatagggt gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtctact gtcaaatacg atggctcaac caacggcagg cagtagagta ctattaaact
      121 agagtttggt agaggtagag ggaatcccta gcgtaacagt gaaatgtgta gattttaggg
      181 agaacaccag cggcgaaggc gctctactgg gccaatactg acactgagag acgaaagcta
      241 agggagcgaa tagg
//
LOCUS       KHUT01002532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002532
VERSION     KHUT01002532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggccttt
       61 caagtcagat gtgaaagccc cgggcttaac ccgggaattg catttgatac tgttgggctt
      121 gagaccggga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002533
VERSION     KHUT01002533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcatga
       61 caagtggatg gtgaaatccc tcggcttaac cgaggaactg ccatccagac tgttttgctt
      121 gaggccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002534
VERSION     KHUT01002534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           96 g           39 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcccgg
       61 aaagtcggat gtgaaatccc ggggctcaac cccggaactg cacccgaaac ttccgggctc
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002535
VERSION     KHUT01002535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtctct
       61 taagttttga gtgacaggtg gtggctcaac taccacaggc cttggaaaac tgggagactt
      121 gaggacttca gagggatgcg gaattccagg tggagcggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gcattctggg aagtaactga cgctcatgtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002536
VERSION     KHUT01002536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           92 g           43 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 caagtgagat gtgaaatccc tcggctcacc cgaggacctg catctcaaac tgccggacta
      121 gagtatggga gaggacaagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cttgtctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002537
VERSION     KHUT01002537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           98 g           48 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggccctt
       61 caagtttgtc gtgaaatctc ccggcttaac cgggagggtg cggcgaagac tgaggggctg
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002538             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002538
VERSION     KHUT01002538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 gacgtcggtt gttgaaagcc cccggctcaa ccggggaact ggcgccgaaa cgccctgtct
      121 tgaggagcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttatctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002539             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002539
VERSION     KHUT01002539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           39 c           75 g           60 t
ORIGIN      
        1 gacataggtt gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtttga
       61 taagttatca gtggcaaatc ggggctcaac ctcgtggaag ctggtaatac tgtcatactc
      121 gagtgcagga gaagttaacg gaactctata tggagcggta aaatgtgacg atatatagaa
      181 gaacaccaat aaggcgaagg cagttaacta ttctgttact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01002540             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002540
VERSION     KHUT01002540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcggat gtgaaacctc caggctcaac ctggagacgc catctgatac tgccgtggct
      121 tgagtccggt agaggagcag ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc gctgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002541
VERSION     KHUT01002541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           90 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggatctt
       61 taagtctggt ttgaaagcag gcggctcaac cgtctgatgt ggctggaaac tggaggtctt
      121 gaatgtgttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggctactagg acatgattga cactgaggca cgagagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002542             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002542
VERSION     KHUT01002542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           98 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggttttc
       61 taagtctggt gtgaaatctc ccggcttaac cgggagggtg cgccggatac tggaaggctt
      121 ggagtgcggg agaggtaagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg cggtgtagac ggcttactgg accgtgactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002543
VERSION     KHUT01002543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           95 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggttac
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaacgg cccctgatac tggtagtctt
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002544
VERSION     KHUT01002544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcccgg
       61 taagtaggga gtgaaatctt gcggctcaac cgcaaaattg ctttctaaac tgccaggctg
      121 gagcacggta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctgga ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002545
VERSION     KHUT01002545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggctgcgcag
       61 aaagtcagat gtgaaatccg ggggctcaac ctccgaattg catccgatac tcctgtgcta
      121 gaggactgga gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacactagt ggcgaaggct gggctctgga cagtatctga cgctgaggca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002546
VERSION     KHUT01002546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcag
       61 tcagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tgctggtctg
      121 gaggtcggga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002547
VERSION     KHUT01002547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tgcagagctc
      121 gagtaccgga gaggatggtg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg accatctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002548
VERSION     KHUT01002548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          100 g           40 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggcccgg
       61 aaagtggggt gtgaaaaccc gaggctcaac ctcgggcgtg cattccaaac tcccgggctc
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgatctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002549
VERSION     KHUT01002549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           89 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagtctgtc gtgaaatctc ccggcttaac tgggagggtg cggcggatac tattgtgcta
      121 gagggtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gcttactgga ccacttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002550
VERSION     KHUT01002550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttcta
       61 caagttggat gtgaaagtcc tgggcttaac ctggggactg catccaaaac tataggacta
      121 gagtagggta gagggaggta gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaataccagt ggcgaaggcg gcctcctgga cgcatactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002551
VERSION     KHUT01002551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggtggcccgg
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaattg cctttgaaac tgccaggctc
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002552
VERSION     KHUT01002552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcaga
       61 taagtcagtg gtgaaatctc taggcttaac ctagaaactg ccgttgatac tatttgtctt
      121 gaatatcatg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttactaca tgttcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002553
VERSION     KHUT01002553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggatat
       61 taagtcagtg gtgaaagccc acagctcaac tgtggaactg ccattgatac tggtattctt
      121 gagtatagat gaggcaggcg gaatttacga tgtagcggtg aaatgcatag atatcgtaaa
      181 gaacacctat agcgaaggca gcttgctaaa ctataactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002554
VERSION     KHUT01002554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggt
       61 taagtccgat gtgaaatccc ggagctcaac tctggaactg cattggatac tattcgacta
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctgga caactcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002555
VERSION     KHUT01002555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           90 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgtgta ggtggcgcgc
       61 taagtcgggt gtgaaatttc ggagcttaac ttcgaaactg cattcgatac tggcgtgctt
      121 gaggactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag agatcatgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgagaca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002556
VERSION     KHUT01002556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           82 g           56 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggcggatttt
       61 taagtcagag gtgaaagccc ggggctcaac cccggagttg cctttgatac tattaatctt
      121 gagtgtagga gagggcaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttgcctggc ctatcactga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002557
VERSION     KHUT01002557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           97 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtaa agcgcacgca ggtggccgcg
       61 caagtgttgt gtgaaagccc cgcgctcaac gcggggaggc cacgacagac tgcacagctt
      121 gagggatgca gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag gccgaaggga gccacctggg catcgcctga cactcatgtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002558             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002558
VERSION     KHUT01002558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggactgt
       61 taagtcgggg gtgaaatccc gaggctcaac ctcggaattg ccttcgatac tggcagtctt
      121 tgagtccggg agaggtgagt ggaactccta gtgtagaggt gaaattcgta gatattagga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgtgac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002559
VERSION     KHUT01002559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           97 g           37 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctgtg
       61 cacgtggggt gtgaaagctg gcggcttaac tgccagacgc cacctcatac ggcacggctc
      121 gagcccgtga gaggtccacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggc acggtgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002560
VERSION     KHUT01002560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           85 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtcatg
       61 taagtctaat gtgaaatctc ggggctcaac cccgaaattg catcggatac tgcgtgacta
      121 gaggactgta gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg aatctctggg cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002561
VERSION     KHUT01002561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          101 g           45 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcgatc
       61 tttgtcaggc gtgaaagccc cgggcttaac ctgggaactg cgcttgggac gggattgctg
      121 gagaccggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002562
VERSION     KHUT01002562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           96 g           40 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggtggcccgg
       61 taagtccgct ctgaaagtca caggctcaac ctgtgaaagc gggtggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002563
VERSION     KHUT01002563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agagcacgta ggtggtctgc
       61 taagtcatgt gtgaaatccc ccggctcaac cggggaattg cgcctgatac tggcaggctt
      121 gaggtcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctcactgga ccgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002564
VERSION     KHUT01002564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggattga
       61 taagtcaggt gtgaaatctc gaggctcaac ctcgaaactg cgcctgaaac tattagtcta
      121 gaatgtcgga ggggacaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctgtctgga cgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002565
VERSION     KHUT01002565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           61 c          105 g           37 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgta ggcggcctgc
       61 tgcgtcggtg ctgaaagcgc ccggcttaac cgggcgaggc gtgccgatac gagcgggctg
      121 gagccttggc gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc caagagctga cgctgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002566
VERSION     KHUT01002566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtcatgt gtgaaagccc ccggctcaac cggggaaggg cacatgaaac tggcaagcta
      121 gagtaccaga gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggc tggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002567             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002567
VERSION     KHUT01002567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           44 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggatccg
       61 ccagtctgac gtgaaatctc caccgctcac cggggagggg ccgttggata ctacggatct
      121 ggagttcggc agaggaccat ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg tggcgaaggc ggtggtctgg gcctgaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01002568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002568
VERSION     KHUT01002568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 caagtctgat gtgaaagccc cgggctcaac ctgggaactg cattggatac tagcgagctt
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002569
VERSION     KHUT01002569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          109 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcaggc
       61 caagcgtggg gtgaaagtcc ggggctcaac tccggatcgg ccttgcggac tggcgtgctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002570
VERSION     KHUT01002570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccat
       61 cgcgtcggga gtgaaaagct ggggcttaac cccagtcctg cttccgatac gggtggacta
      121 gaggtaggta ggggagatcg gaattcctag tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002571
VERSION     KHUT01002571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          104 g           42 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggatgg
       61 caagtcggtc gtgaaatccc acggctcaac tgtggggggt cggccgaaac tgctgttctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg catctcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002572
VERSION     KHUT01002572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           90 g           57 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggctgtttgt
       61 taagtctgat gttaaagact ggggctcaac ctcggaaatg cattggatac tggcagactg
      121 gagtgcagta gaggctagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaggcg actagctggg ctgtaactga cgctgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01002573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002573
VERSION     KHUT01002573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          102 g           44 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggactta ctgggcgtaa agcgtgggta ggtggtcgat
       61 gatgtgccgt gtgaaagcgc cgcgcttctc gcggcgaggt cgcggtagac acatcgactg
      121 gaggcacgga ggggggcgta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgcaagcg gcgccctggc cgtgacctga cactgagcca cgacggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002574
VERSION     KHUT01002574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggttagg
       61 taagtcaggt gtgaaatctc agggctcaac cctgaaactg cacttgatac tgcttgacta
      121 gaggactgga gaggtgatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagc ggcgaaggcg gatcactgga cagttcctga cgctcaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002575
VERSION     KHUT01002575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttta
       61 taagtctgat gtgaaatccc cgggctcaac ctgggaattg cattggagac tgtaaggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002576             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002576
VERSION     KHUT01002576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gcaagtcaga agtgaaatct cattgctcaa cgatggggct gcttttgata ctgcggatct
      121 ggagaccggt agaggctggt agaattacag gtgtagcggt ggaatgcgta gatatctgta
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002577
VERSION     KHUT01002577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcatgta ggcggtttgt
       61 taagtccgtt gttaaagccc ccggctcaac tgggggaggt caatggagac tagcagactt
      121 gagggcaaca gaggcacatg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gtgtgctggg ttgcaactga cgctgagatg cgaaagtgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002578
VERSION     KHUT01002578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctcct
       61 caagtcgggg gtgaaatcct ctcgcttaac gagagaattg cctccgatac tggggagctg
      121 gagggggata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tccccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002579
VERSION     KHUT01002579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           95 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggtggtttgt
       61 cgcgtctgct gtgaaaatcc ggggctcaac cccggacttg cagtgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccattactga cactgagaag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002580
VERSION     KHUT01002580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccgac
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tggtcggctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002581
VERSION     KHUT01002581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           50 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtccgt
       61 taagtgtaga gtgaaagctc cgggctcaac tcggaaattg ctctgcatac tggcggactc
      121 gaggacggaa gaggtttgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccat ggcgaaggca gcaaactggt ccgtatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002582             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002582
VERSION     KHUT01002582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          101 g           40 t
ORIGIN      
        1 gacagagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggaccag
       61 gcaagtctgg agtgaaatct cgccgctcaa cggcgaggct gctttggaaa ctgcgggtct
      121 ggagaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002583             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002583
VERSION     KHUT01002583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           92 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagcgcgta ggcggcgctg
       61 caagtcgaag gtgaaatccc accggctcaa ccgtggaatc gcctgcgaaa ctgcagcgct
      121 tgagggagct aggggcatgt ggaacttccg gtggagcggt gaaatgtgtt gatatcggag
      181 ggaacgccag tggcgaaagc ggcatgctgg atctcaactg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01002584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002584
VERSION     KHUT01002584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           97 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaatcc agggcttaac tctggactgg cttccgatac gggcagacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002585
VERSION     KHUT01002585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           86 g           47 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattca ctgggcttaa agagttcgta ggcggtctag
       61 acagttgggt gtgaaagccc tcggctcaac cgaggaactg cgctcaaaac tactagactt
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg tctctactga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002586
VERSION     KHUT01002586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          104 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcaggc
       61 caagtgtgcg gtgaaagtcc ggggctcaac ctcggatctg ccgtgcagac tggtttgcta
      121 gagcactgta gaggcgagtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gctcgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002587
VERSION     KHUT01002587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taagtcagag gtgaaatccc ggggctcaac tccggaactg cctttgaaac tgcatcgctt
      121 gaatgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002588
VERSION     KHUT01002588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtttgg
       61 taagtctgcc gtgaaaaccc agggcttaac cctgggcgtg cggtggaaac tgccaggcta
      121 gaggatggta gaggcgagtg gaattccgag tgtagcggtg aaatgcgcag atattcggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccatacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002589
VERSION     KHUT01002589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          105 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctgg
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgctggactg
      121 gagcatgtca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg acatggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002590
VERSION     KHUT01002590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggttga
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tattgatctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgtaggca gcttactaca cgatcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002591
VERSION     KHUT01002591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           82 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggatggt
       61 taagtccaaa gtgaaaggtc agagctcaac tctggcactg cttgggatac tggccgtctt
      121 gagtaccgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca gctttctgga cggaaactga cgctcatgtt cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01002592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002592
VERSION     KHUT01002592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          105 g           42 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccgct
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaggtg cgcttgggac tgggtggctg
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002593
VERSION     KHUT01002593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctagt
       61 tatgtcggat gtgaaagccc ttggctcaac tgaggaagcg cacccgaaac tgactagctg
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002594
VERSION     KHUT01002594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           98 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcaga
       61 taagtcagct gtgaaaacct cgggcttaac tcgaggcctg cagttgaaac tgtctggctg
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccactactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002595
VERSION     KHUT01002595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggtgcag
       61 atagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac tcctgtactc
      121 gaggaagcta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002596
VERSION     KHUT01002596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           38 c           83 g           59 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggtggtttga
       61 taagttagat gtgaaagccc tgggctcaac ccgggaattg catttaagac tgttgaactt
      121 gaaggtcgaa gaggtaagca gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacatcaat ggcgtaggca gcttactggt cgatacttga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002597
VERSION     KHUT01002597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggct gtgaaaactc ggggctcaac cccgagcctg cagtcgatac gggcaggcta
      121 gagtccggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccggaactga cgctaaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002598
VERSION     KHUT01002598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           87 g           47 t
ORIGIN      
        1 tacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtgcctt gtgaaatccc atggctcaac catggaactg ctgggcatac tggacgtctt
      121 gagccatcca ggggtcgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaagaca ggcgactggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002599
VERSION     KHUT01002599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtagg
       61 taagtccgtg gtgaaatctt ccgacttaat cgggaaactg ccatggatac tctctatctt
      121 gaatgtcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctaactacg cgataattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002600
VERSION     KHUT01002600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggatatt
       61 taagtcagat gtgaaaactg gcggctcaac cgtgagactg catctgatac tggatatctt
      121 gagggtaaga gagggcagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actgcctggc ttacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002601
VERSION     KHUT01002601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggcggctagg
       61 caagtcagat gtgaaagccc agggctcaac cctgggactg catttgaaac tgcttagcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002602
VERSION     KHUT01002602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggatagt
       61 tcagtcagag gtgaaatccc ggggctcaac ctcggaactg cctttgatac tggccatctt
      121 gagtatggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002603
VERSION     KHUT01002603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggaatg
       61 taagtcagtg gtgaaatctc tgagcttaac tcagaaactg ccattgatac tatgtttctt
      121 gaattttctg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctgactaca gggatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002604
VERSION     KHUT01002604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           93 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctcg
       61 taagacaggt gtgaaatccc cgggctcaac ctgggaactg cgcttgtgac tgcgaggctg
      121 gagtgcggaa gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggt ccagcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002605
VERSION     KHUT01002605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c          101 g           40 t
ORIGIN      
        1 gacggaaggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggcttgg
       61 tgggtctggt gtgaaagccc cgggctcagc ccgggaaggg cactagaaac tgccaagctg
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002606
VERSION     KHUT01002606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           94 g           45 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggcataa
       61 caagttggat gtgaaagctc ccggcttaac tgggagaggt cattcaaaac tgttaggcta
      121 gaggatggaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tcacacctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002607
VERSION     KHUT01002607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccatg
       61 taggtccgat gtgaaaactg ggggctcaac ctccagctgt cgtcggaaac catgtggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002608
VERSION     KHUT01002608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcatgca ggcggaagcc
       61 aaagtcggac gtgaaagctc ctggctcaac taggagaggc cgttcgaaac tgggcatctg
      121 gaggctggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccagtcctga cgctcagatg cgaaagctag
      241 gggagagaac ggg
//
LOCUS       KHUT01002609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002609
VERSION     KHUT01002609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           97 g           44 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggtggtcatt
       61 taagttcggc gtgaaagctt ctggctaaac cgggagaggt cgtcggagac tggatggcta
      121 gagggtgata gaggcgggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acccgctggg tcacacctga cactgagacg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002610
VERSION     KHUT01002610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttaca
       61 taagttggaa gtgaaagccc cgggcttaac ctgggaactg ctttcaaaac tgtgtgacta
      121 gagtgcagca gaggatagtg gaatttctag tgtagcggtg aaatgcgtag atattagaag
      181 gaacaccagt ggcgaaggcg actgtctggg ctgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002611
VERSION     KHUT01002611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggtggtcttt
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaaatg ccattgaaac tgtaagactt
      121 gaggatgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcatatctga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002612
VERSION     KHUT01002612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc atggcttaac catggaactg cttggcatac tggcggtctt
      121 gagccactta ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002613
VERSION     KHUT01002613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           87 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc acggcttaac cgtggaactg cttggcatac tggcggtctt
      121 gagcaaccta ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtttgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002614
VERSION     KHUT01002614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacttg
       61 tcagtcaggg gtgaaatccc gaggcttaac ctcggaactg cctttgatac tgcaagtctt
      121 gaggatgaga gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggc tcatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002615             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002615
VERSION     KHUT01002615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           97 a           24 c           64 g           69 t
ORIGIN      
        1 tacgggagga gcgagcatta ttcggaatga ttaggcgtaa agggtttgta ggtggttttt
       61 taagttgaaa aaaacaaatt aaagctcaac tttataaatt ttttcaaaac tgaaaaactt
      121 gagtataaat agaggataat ggaatttcta ttggagggat aaaatatgtt gataatagaa
      181 ggaagatcaa aagcgaaggc aattatctgg gtatatactg acactgagaa acgaaagctt
      241 gggtagcgaa cggg
//
LOCUS       KHUT01002616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002616
VERSION     KHUT01002616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           89 g           56 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttga
       61 taagtttgat gtgaaatctc tgggctcaac ccagagtctg catcgaaaac tgtcgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccattactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002617
VERSION     KHUT01002617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           86 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggattgc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggctgtctt
      121 gagccactta ggggcgaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggtcgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002618
VERSION     KHUT01002618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggagtta ctgggcgtaa agcgcacgca ggcggtcgtg
       61 taagtttggg gtgacagctc ccggctcaac tgggagagga ccttgaagac tgcaggactt
      121 gagggccaga gagggacacg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca gtgtcctggc tggtacctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002619
VERSION     KHUT01002619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttggg
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaactg cattcgatac tgcccgacta
      121 gagtatggga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccaat ggcgaaggca gcttcctggc ccaatactga cactcaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002620             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002620
VERSION     KHUT01002620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 caagccgacg gtgaaatctc cacgctcaac gtggaggggt ccgtcggaac tgtcgtgact
      121 agagtccggt aggggagtgt ggaatttccg gtgtagcggt gaaatgcgca gagatcggaa
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002621
VERSION     KHUT01002621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           78 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggagcgta ggcggcttat
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgatgagctt
      121 gaatatagtt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaag ctattattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002622
VERSION     KHUT01002622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           97 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agggcgtgta ggcggttttg
       61 tatgtctggc gtgaaagccc cgggctcacc tcgggaagtg cgttggaaac tgcaagacta
      121 gagtcccgga gaggaaggtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002623
VERSION     KHUT01002623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcacg
       61 tatgtctgct gtgaaagtac acggctcaac cgtgtgaagt cggtagaaac tgcgagactt
      121 gaggctggga gaggttggtg gaattccctg tgtagcggtg aaatgcgtag atatagggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagccctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002624
VERSION     KHUT01002624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c          100 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtccgt
       61 tacgtccgtt gtgaaagctc ccggctcaac tgggagaggt cttcggatac gggcggactc
      121 gaggaaggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctttcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002625
VERSION     KHUT01002625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggtggttgga
       61 taagttatct gtgaaatccc tgggctcaac ctgggcaggt cagataatac tgtttaactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002626
VERSION     KHUT01002626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggatgta
       61 tgtgagtgat gtgaaagccc cgggcttaac ctgggaagcg cattgctgac tgtacgacta
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002627             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002627
VERSION     KHUT01002627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          103 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggggat
       61 taagtgagag gtgaaaggct ggggctcaac cccatgcagt gcctctcata ctggtcttct
      121 tgagggcggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccgttcctg acgctgaggc gcgaaagctg
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002628
VERSION     KHUT01002628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           79 g           61 t
ORIGIN      
        1 tacgtagggg gctagagttg tccggattta ctgggtgtaa agggtgctca ggtggacttg
       61 taagtcagag gtgaaatttc aaagcttaac tttggagctg cctttgatac tgcaagcctt
      121 gagtttgaga gagggtaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttacctggc tcaaaactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002629
VERSION     KHUT01002629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagt
       61 taggtccact gtgaaaactc gaggctcaac ctcgagacgt cggtggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002630
VERSION     KHUT01002630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          103 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtgact
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgggtcactg
      121 gagcagatca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg atcttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002631             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002631
VERSION     KHUT01002631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggttcgg
       61 taagttgggc gtgaaatcta cccggctcaa ctgggagagg tcgtccaata ctaccggact
      121 tgagagcagt agaggaagat ggaattcccg gtgtagtggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaagc gatcttctgg actgtttctg acgctcagac gcgaaagcta
      241 gggtagcaaa cggg
//
LOCUS       KHUT01002632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002632
VERSION     KHUT01002632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           90 g           51 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgtgta ggcggtttag
       61 tgtgtgggaa ttgaaagccc ggggctcaac tccggatccg gttcccaaac tgctagactc
      121 gagcgtatca gaggacagtg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actgtctggg gtattgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002633
VERSION     KHUT01002633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagt
       61 taagtccgtg gtgaaatctc tgagcttaac tcagaaactg ccatggatac tatctgtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca cagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002634
VERSION     KHUT01002634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           95 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcttgta ggcggtttgt
       61 taagtctggt gtgaaatctc ccggctcaac cgggaggggt catcggatac tggcagactt
      121 gagacgggta gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002635
VERSION     KHUT01002635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           41 c           89 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcctgt
       61 taagtctggt ttgaaagtag gtcgcttaac gatctaatgt ggctggaaac tggggggctt
      121 gaatgatttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg aattgattga cactgaggca cgagagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01002636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002636
VERSION     KHUT01002636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcggatatt
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tatttatctt
      121 gaattctgtt gaggtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaag cagacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002637
VERSION     KHUT01002637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           90 g           57 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggtggtttct
       61 taagtttgac gtgaaagctc ctggcttaac tgggagaggt cgttgaatac tggggaactt
      121 gaggcaatga gagggatgta gaattcacgg tgtagtggtg gaatgcgtag atatcgtgag
      181 gaataccagt ggcgaaagcg gcatcctggc attagcctga cactcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002638
VERSION     KHUT01002638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          104 g           36 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgcg ggcggacatg
       61 caagtcggga gtgaaagacc ggggctcaac cccggacccg ctttcgagac tgcatgtcta
      121 gggaccggta ggggcaggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002639
VERSION     KHUT01002639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcatgta ggcgggtcgt
       61 taagttgggg gtgaaagccc gaggctcaac ctcggaattg ccttcaaaac tggcgacctg
      121 gagcatgata gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actcgctgga tcattgctga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002640
VERSION     KHUT01002640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggtgac
       61 taagtcaggg gtgaaatgcc gaggctcaac ctcggaactg cctctgatac tggtcgcctg
      121 gagttcggga gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gcttactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002641
VERSION     KHUT01002641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           87 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgcacgta ggcggcgtcg
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tgcaatgcta
      121 gaatattgga gaggcaagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acttgctgga caattattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002642
VERSION     KHUT01002642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           72 g           67 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactaa
       61 taagtcagtg gtgaaatctt acagcttaac tgtaaaattg ccgttgatac tgttagtctt
      121 gaatatagtt ggggtggatg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat cgcgtaggca gttcaccaaa ctattattga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002643
VERSION     KHUT01002643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccagg
       61 caagtcggat gtgaaagccc atggctcaac catggaagtg cattcgaaac tgcttggctg
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg accttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002644
VERSION     KHUT01002644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           84 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctggg
       61 taagtttgat gtgaaatctc cgggctcaac ccggaaaatg cattgaatac tatccagcta
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caaatcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002645
VERSION     KHUT01002645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           99 g           40 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtccgc
       61 taagtctgat gtgaaagccc ggggctcacc cccggaaggg cattggaaac tggcgaactc
      121 gagtcccgga gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggc ggcgaaggcg gccctctgga cggtgactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002646
VERSION     KHUT01002646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggtttgt
       61 taagtcaggt gtgaaatccc ggggctcaac cccggaactg catctgatac tggcagacta
      121 gagtgcggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga ctgacactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002647
VERSION     KHUT01002647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggtttag
       61 taagtcggtc gtgaaatccc ttggcttaac caaggaactg cgatcgaaac tgttagactc
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002648
VERSION     KHUT01002648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcggac gtgaaatccc tcggctcaac cggggaactg cgtccgatac tggttggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002649
VERSION     KHUT01002649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggctgtt
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgggcagcta
      121 gagttgagta gaggagaggg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacaccagt ggcgaaggcg cctctctgga ctcaaactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002650
VERSION     KHUT01002650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaactc agggcttaac cctgagcctg cttccgatac gggcagacta
      121 gaggtatgca ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002651             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002651
VERSION     KHUT01002651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           50 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggatcgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctggcgatct
      121 ggaggaatgg agaggtgagt ggaattcctg gtgtaccggt gaaatgggta gatatcagga
      181 ggaacaccca tggcgaaggc agctcactgg ccattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002652             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002652
VERSION     KHUT01002652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          102 g           39 t
ORIGIN      
        1 gacagagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatctg
       61 caagtccgta agtgaaatcc cgccgctcaa cggcggggct gcttgggaaa cggcaggtct
      121 ggggaccggt agaggtcggt agaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaatacccg tggcgaaggc ggccgactgg gccggatccg acgctgatgc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002653             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002653
VERSION     KHUT01002653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           53 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcagat
       61 caagtcagtt gtgaaatctc tgagcttaac tcagaactgt caactgatac tgttctgcta
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgagtgc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01002654             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002654
VERSION     KHUT01002654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           96 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtgtgt
       61 cacgtcgatc gtgaaaactt tcagcttaac tgggagcttg cggtcgatac gggcatcact
      121 ggagttcggc aggggagact ggaattcaag gtgtagcggt gaaatgcgca gatatcttga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002655
VERSION     KHUT01002655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           95 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcgaac gtgaaatccc tgggctcaac ccaggaactg cgtccgatac tggttggctt
      121 gaatccggga gagggacgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg acgtcctgga ccggcattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002656
VERSION     KHUT01002656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 taagtcggat gtgaaatctc agggctcaac cctgaaattg catccgatac tgacggactt
      121 gaggcaggta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002657
VERSION     KHUT01002657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 aaagtttgat gtgaaagctc ggagctcaac tccgaaatgg cattgaatac tatccggctt
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01002658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002658
VERSION     KHUT01002658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           97 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctcgg
       61 atagtcggat gtgaaaaccc agggctcaac cctgggcctg cacccgatac tcccgggcta
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002659
VERSION     KHUT01002659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgggg
       61 caagtcaggt gtgaaatccc ggagctcaac tccggaactg cacttgatac tgccttgcta
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002660
VERSION     KHUT01002660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           51 t
ORIGIN      
        1 gacgaactgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgat
       61 caagtcaggg gtgaaatcct gcagcttaac tgtagaagtg cctttgatac tggtcgtctc
      121 gagggaggta ggggtgtgtg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002661
VERSION     KHUT01002661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttcgg
       61 tcagtcagat gtgaaagccc cgggcttaac ctgggaatgg catttgatac tgccggactg
      121 gagtgcgata gaggacagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccgat ggcgaaggca gctgtctggg tcgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002662
VERSION     KHUT01002662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtctgct gtgaaaactc ggggcttaac tccgagcctg cagtgggtac gggcagacta
      121 gagtatggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccataactga cgctgagaag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002663
VERSION     KHUT01002663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           94 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatc
       61 atagtctgtg gtgaaatccg gcagcttaac tgtcgaattg ccatggatac tgggtgtctc
      121 gagggaggca ggggtgcgcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaactccggt ggcgaaggcg gcgcactggt cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002664
VERSION     KHUT01002664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          101 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctga
       61 taagtagggg gtgaaatctc gcggctcaac tgcgaggctg cctcctaaac tgtcgggctc
      121 gagcacagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ctgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002665
VERSION     KHUT01002665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           75 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctt caagcttaac ttggaaactg ccattgatac tatttgtctt
      121 gaatatcctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactacg ggattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002666
VERSION     KHUT01002666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           82 g           52 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgttttaa ggtggtttgg
       61 taagtctact gttaaatatc agggcttaac cctgaacagg cagtggaaac tagcaagctt
      121 gagtacggta ggggcaaagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccaaa ggcgaaagca ctttgctggg ccgaaactga cactgataga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01002667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002667
VERSION     KHUT01002667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           92 g           45 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggtccgc
       61 taagtcggat gtgaaattcc ccggctcaac cggggaacgg caatcgatac tggcgggctt
      121 gaggtccgga ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccagt ggcgaaagcg gctcactggc cggaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002668
VERSION     KHUT01002668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagttcggt gtgaaatccc ctggctcaac tggggaactg caccggaaac tgacagacta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002669
VERSION     KHUT01002669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 taagttggat gtgaaatctc ggggctcaac cccgaaactg cattcaatac tgctctgctc
      121 gagtactgga gaggagatcg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002670
VERSION     KHUT01002670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           50 c          100 g           50 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgttc gtgaaatccc gtggctcaac tgcgggcttg cggacgatac gggcaggcta
      121 gaattcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatattga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002671
VERSION     KHUT01002671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagtttggt gtgaaatctc ccggctcaac cgggaggctg catcgaatac tggaatgcta
      121 gagggtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccacttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002672
VERSION     KHUT01002672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           46 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggctgtg
       61 caagtcgtgt gttaaagccc ctggctcaac tgggggaggt catgcgagac tgcgcggctg
      121 gaggacagta gaggcacatg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gtgtgctggg ctgtttctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002673
VERSION     KHUT01002673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           85 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcttc
       61 caagtcagtg gtgaaagcct ggagctcaac tccagaactg ccattgaaac tgttagactt
      121 gagtacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcgtaactga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002674
VERSION     KHUT01002674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccccg
       61 taagtggtgt gtgaaatctc ctggctcaac cagggaattg cgctccaaac tgcagggctc
      121 gagggcagta gaggaagctg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgtt ggcgaaggcg agcttctggg ctgttcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01002675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002675
VERSION     KHUT01002675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           85 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgggg
       61 aaagtcagat gtgaaatccg gaggctcaac ctccgaattg catccgatac tccctcgcta
      121 gagagttgta gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgc agcgaaggcg aagctctggg caatttctga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002676
VERSION     KHUT01002676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcacct gtgaaatctc caagctcaac ttggagcctg caggcgaaac tgccgtgctg
      121 gagtatggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002677
VERSION     KHUT01002677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgaacgta ggcggtgttc
       61 catgtcagag gtgaaagcct ggggctcaac tccagaattg cctttgaaac ggggacgctt
      121 gagtccgaga gaggatggcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggc tcggtactga cgctcaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002678
VERSION     KHUT01002678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          101 g           45 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccgat
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaatgg cgcttgggac tggttggctt
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002679
VERSION     KHUT01002679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgtgta ggtggcgcgg
       61 taagtcgggt gtgaaatctc ggagcttaac tccgaaattg cattcgatac tgccgtgctc
      121 gaggactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgagaca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002680
VERSION     KHUT01002680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacagaggtg tcaaacgttg tccggaatta ttgggcgtaa agggcgcgta ggcggccggg
       61 taagtgagat gtgaaagctc tgggctcaac ccaggaattg catttcatac tgctcggctt
      121 gagattggta gaggatggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacctgt ggcgaaggcg gctatctggt ccaattctga cgctgaggcg cgaaaggtag
      241 gggagcaaac agg
//
LOCUS       KHUT01002681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002681
VERSION     KHUT01002681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggctag
       61 taagtccgtg gtgaaatctc tgagcttaac tcggaaactg ccatggatac tattaatctt
      121 gaatattgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctgactacg catatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002682
VERSION     KHUT01002682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          102 g           47 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcttg
       61 taagtctgtg gtgaaagccc ggggctcaac cccgggtctg ccgtggatac tgtgagactt
      121 gagtgctgta gaggcaggta gaatatcggg tgtagcggtg gaatgcgtag agatccgata
      181 gaagaccggt ggcgaaggcg gcctgctggg cagtaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002683
VERSION     KHUT01002683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           89 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgtgta ggtggcgccg
       61 taagtggggt gtgaaatttc ggagcttaac tccgaaactg cattccatac tgcggtgctt
      121 gaggactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgagaca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002684
VERSION     KHUT01002684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taagtcaaat gtgaaatccc cgagcttaac ttgggcattg cattcgatac tggcaagcta
      121 gagtatggga gaggaaggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccgat ggcgaaggca gccttctggc ctaatactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002685
VERSION     KHUT01002685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcagct gtgaaatctc cgggctcaac tcggagcctg cagttgaaac tgccgtgctt
      121 gagtatggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002686
VERSION     KHUT01002686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggtcaac
       61 taagtcatac gtgaaatccc tcggcttaac cggggaactg cgcctgatac tggttgactt
      121 gagtttggga gagggacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga ccaatactga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002687
VERSION     KHUT01002687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           88 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatca ctgggcgtaa agggcgcgta ggcggctctg
       61 caagtcaaat gtgaaagccc acggcttaac cgtggaattg catctgaaac tgcatggctt
      121 gagtatggga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt agcgaaggcg actatctggc ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002688
VERSION     KHUT01002688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgggta ggcggtgact
       61 taagtcatgc gtgaaagctc ctggctcaac tgggagaggt cgtgtgaaac tgggtcacta
      121 gaggcaggta gaggcaaacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gtttgctggc cctgacctga cgctcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002689
VERSION     KHUT01002689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta ctcggaatca ctgggcgtaa agggtgcgta ggctgcctat
       61 taagttggat gtgaaagccc tcggcttaac cgaggaactg cgtccaaaac tggtgggctt
      121 ggggttggta ggggacagtg gaactccagg tggagcggtg aaatgcgtag agatctggag
      181 gaactccggt ggcgaaagcg actgtctggg ccatacccga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002690
VERSION     KHUT01002690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttga
       61 taagtcgagt gtgaaatccc cgggcttaac ctgggacctg cattcgaaac tgtcttgcta
      121 gagtatggga gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002691             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002691
VERSION     KHUT01002691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gctagcgtta tccggaatta ttgggcgtaa agggtgcgta ggcggtccta
       61 caagtcagag gtgaaaggct acggctcaac cgtagtaagc ctttgaaact gtaggacttg
      121 agtgcaggag aggagagtgg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtt gcgaaggcgg ctctctggac tgtaactgac gctgaggcac gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01002692             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002692
VERSION     KHUT01002692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           94 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggtggtggat
       61 taagttgtgg gtaaaaggca ttggctcaac caatgtaagc ctgcaaaact ggtacacttg
      121 agtgcaggag aggggagtgg aattcccggt gtagcggtgg aatgcgtaga tatcgggagg
      181 aacaccagtg gcgaaggcga ctctctggcc tgtaactgac actgaggcgc gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01002693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002693
VERSION     KHUT01002693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gatggctgat
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaatgg catccgatac tggttggcta
      121 gagtgcggta gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gctctctgga ccagcactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002694
VERSION     KHUT01002694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          100 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgcac
       61 caagtcaggc gtgaaagccc tgggcttaac ccaggaattg cgtttgagac tggcgtgctg
      121 gagtgcagga gaggtcggcg gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaacaccggt ggcgtaggcg gccgactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002695
VERSION     KHUT01002695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtctgac gtgaaatctc gaggctcaac ctcgagcggt cgttggaaac cgtctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002696
VERSION     KHUT01002696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           94 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagttggat gtgaaaactg tgggcttaac ccatagcctg catccaaaac tagcaggcta
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagagtctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002697
VERSION     KHUT01002697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           80 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgca ggcggattgg
       61 taagtcagtg gtgaaatacc cgtgcttaac atgggaactg ccattgatac tatcagtctt
      121 gaatgttgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaaa caaatattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002698
VERSION     KHUT01002698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtaga gtgaaagctc cgggctcaac ccggaaattg ctctgcatac tggcagactt
      121 gagggatgca gaggtttgtg gaattcctgg tgtaacggtg aaatgtgttg atatcaggag
      181 gaacacccat ggcgaaggca gcaaactggg catcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002699
VERSION     KHUT01002699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          100 g           44 t
ORIGIN      
        1 tacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcctgt
       61 tgcgtccgct gtgaaatccc ggggctcaac cccgggcttg cagtggatac gggctggcta
      121 gaggcaggca ggggagaacg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002700
VERSION     KHUT01002700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccag
       61 acagtctgtg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tgctggcctc
      121 gaggagggta ggggcatctg gaacggtcgg tggagcggtg aaatgcgttg atatcgaccg
      181 gaactccgat ggcgaaggca aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002701
VERSION     KHUT01002701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           78 g           57 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggcccta
       61 taagtcagtg gtgaaatctc ctcgcttaac gaggaaattg ccattgatac tgtagggctt
      121 gagtacagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaag ctgtaactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01002702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002702
VERSION     KHUT01002702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatggc
       61 taagtacttt gtgaaatccc acggctcacc cgtggaactg cttggtatac tggtcgtctt
      121 gagccactta ggggttgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggcaactggg agtgtgctga cgctgagaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002703
VERSION     KHUT01002703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagtctgct gtgaaagccc cgggctcaac ctgggaatgg cagtggatac tggcgagcta
      121 gagtgtgtca gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccatctggg acaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002704
VERSION     KHUT01002704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          102 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 cgcgtccggg gtgaaatccc acggctcaac cgtggaacgg cctcggatac gggcggcctc
      121 gaggggggta ggggcatctg gaactgtgag tggagcggtg aaatgcgttg atattcacag
      181 gaactccggt ggcgaaggcg aggtgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002705
VERSION     KHUT01002705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtcaaag gtgaaatccc tcggcctaac cgaggaactg cctttgaaac tgcttggctt
      121 gaggccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002706
VERSION     KHUT01002706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           99 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggctcgg
       61 taagtcggat gtgaaaacct gaggctcaac ctcaggcgtg cattcgatac tgccgagctc
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01002707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002707
VERSION     KHUT01002707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggctat
       61 taagtcagcg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tattagtctt
      121 gaatatcgtt gaggttggcg gaatatatca tgtagcggtg aaatgcatag atatgatata
      181 gaacaccgat tgcgaaggca gctaactaag cgatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002708
VERSION     KHUT01002708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           89 g           49 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 caagtcaact gtgaaagccc agagcttaac tctggaagtg cagctgagac tgttgaattt
      121 gagtatagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctataactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002709
VERSION     KHUT01002709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccgttgatac tatcaatctt
      121 gaatatcgcg taggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttacttca cgatcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002710
VERSION     KHUT01002710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           86 g           46 t
ORIGIN      
        1 gacgagcctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac taccgtgctc
      121 gaggaagata gaggtgagcg gaactcacgg tggagcggtg aaatgcgttg atatcgtgag
      181 gaacacccgt ggcgaaagcg gctcactggg tcttttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002711
VERSION     KHUT01002711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          102 g           38 t
ORIGIN      
        1 tacagggggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggtccac
       61 tacgtgccgt gtgacagacg tcggcttaac cgacgtaggc cagggtacac gggtgaactt
      121 gaggcagaga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcatcctggc tctgtactga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002712
VERSION     KHUT01002712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          100 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 taagtagggg gtgaaatctc acggctcaac tgtgaggctg ccttctagac tgtcgggctt
      121 gagcacagta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatctgt ggcgaaggcg gccagctggg ctgttgctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002713
VERSION     KHUT01002713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           93 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggattta ttgggcgtaa agcgcgtgta ggcggcttct
       61 taagtctgat gtgaaagccc tgggctcaac ccgggaagtg cattggaaac tgggaaactt
      121 gaatacggga gagggtagtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002714             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002714
VERSION     KHUT01002714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          108 g           36 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcta
       61 ggaaggcggc ggtgaaagtc cggggctcaa ccccggatcg gccgtggcga ctcctgagct
      121 cgagcacagt agaggcgggt ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcccgctgg gctgtggctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002715
VERSION     KHUT01002715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           96 g           42 t
ORIGIN      
        1 gacagaggag gcaagcgttg ttcggaatca ttgggcttaa agggcgtgca ggcggcggcg
       61 caagtgtcga gtgaaatccc atcgctcaac gatggaacgg ctcggcaaac tgcgtcgctt
      121 gaggtcggta gaggtgctcg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcctga ggcgaaagcg gggcactggg ccgattctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002716
VERSION     KHUT01002716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggttggta ggtggtcttg
       61 tttgtctaag gtgaaatccc atggcttaac tgtggaagtg ccttagaaac ggcaagacta
      121 gagtcctaga ggggtttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcaaactgga taggcactga cactcaacaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002717
VERSION     KHUT01002717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggtgcgta ggcggttcta
       61 tgagtgtgat gtgaaagccc tgggctcaac ctaggaagtg catcgcaaac gatagaactg
      121 gagtatgtga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc acaatactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002718
VERSION     KHUT01002718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           80 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcca
       61 taagtcagtg gtgaaataca atagcttaac tattgaggtg ccattgatac tgcggaactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaga ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002719             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002719
VERSION     KHUT01002719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           47 t
ORIGIN      
        1 cacgtaggag acgagcgtta tccggattta ctgggcgtaa agcgcgggtc ggtggggtct
       61 taagtcatgc gtgaaagctc tcggcttaac cgggagaggt cgtgtgaaac tgggattctc
      121 agaggtaggt agaggcagat ggaattccgg gtggagtggt gaaatgcgta gagatccgga
      181 ggaacaccag tggcgaaggc gatctgctag gcctgacctg acactcagcc gcgacagcat
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002720
VERSION     KHUT01002720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          111 g           34 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcggac tgccgggctc
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002721             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002721
VERSION     KHUT01002721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           38 c           73 g           64 t
ORIGIN      
        1 tacgtaggtt gcaagcgtta tccggaatta ctgggcgtaa agcgtccgta gcaggatttg
       61 taagtctgtt ttcaaagact ctggctcaac tggaggaagg gaatagatac tgcatttcta
      121 gaagttttta gaggatagtg gaatttccgg tggagaggtg aaattcgttg atatcggaaa
      181 gaacgccgaa agcgaaagca gctatctata aaaacttgac tgtaatggac gaaagcttgg
      241 gtagcaaacg gg
//
LOCUS       KHUT01002722             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002722
VERSION     KHUT01002722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           47 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggctccta ggcggttcag
       61 taagtcgact gtgaaaatcc aaggctcaac cttgggacgc cagtcgatac tgctgtgact
      121 cgagttcggt agaggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cggcgaaggc agcactctgg gccgatactg acgctgaaga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002723
VERSION     KHUT01002723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggctgat
       61 cgcgtcggtt gtgaaagctc agggctcaac cctgagacgc caatcgatac ggatcggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01002724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002724
VERSION     KHUT01002724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 gacagagggc tcaaacgttg ttcggaatta ctgggcataa agcgcacgca ggcggcttgc
       61 taagtcgggt gtgaaatccc ttggctcaac cgaggaactg cattcgatac tggtaggctc
      121 gaggacggga ggggtaagcg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactggc ccgtatctga cgctcatgtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002725
VERSION     KHUT01002725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggattgg
       61 taagtacctt gtgaaatccc acagctcaac tgtggaactg ctgggtatac tgcttatctt
      121 gagccactta ggggctgtcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaac ggtgaaaaca gacagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002726
VERSION     KHUT01002726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          102 g           39 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcacgc
       61 taagtacacc gtgaaatctc cctgctcaac ggggagggtg cgatgtagac tggcgagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002727
VERSION     KHUT01002727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c           99 g           39 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgta ggcggttgcg
       61 tgcgtcggcg ctgaaatccg tgggctcaac ccacgggcgc gtgctgatac gacgcgactc
      121 gaggctcgga gagggtggga gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaatacccgt ggcgcaagcg cccacctggc cgagacctga cgctaaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002728
VERSION     KHUT01002728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agggcgtgca ggtggctgtg
       61 taagtggtgc gtgaaagcgc tcggctcaac cgggcgaggg cgtgccaaac tgcacaacta
      121 gaaacaggca gaggaaagtg gaattcgggg tgtagtggtg aaatgcgtag agatcccgag
      181 gaactcctgt ggcgaaggcg actttctggg cctgctttga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002729
VERSION     KHUT01002729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggttcgg
       61 taagttgggc gtgaaatctc ctggctcaac taggagaggt cgttcaatac taccgggcta
      121 gagagtggta gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga ccatttctga cgctcagacg cgacagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002730
VERSION     KHUT01002730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatta ctgggcttaa agcgcacgta ggcggcgccg
       61 caagtgcggg gtgaaatccc acggcttacc cgtggaactg ccttgcagac tgcggtgctc
      121 gaggatggta ggggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002731
VERSION     KHUT01002731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           57 c          108 g           41 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcttca
       61 taagtctggc gtgaaagccc ccggctcaac cggggagggt cgtcggagac tgtggagctt
      121 gagggcggta ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccagctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002732
VERSION     KHUT01002732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           87 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggggcga
       61 taagtcagtg gtgaaatcct gcagcctaac tgtagaactg ccattgatac tgtcgtcctt
      121 gagtgcgctc gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agtgaagaca gctcacgagg gcgaaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01002733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002733
VERSION     KHUT01002733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          101 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgg
       61 tcagtcggat gtgaaagccc ggggctcaac cccggaacgg catccgagac ggccaggctg
      121 gagaccggga gaggagagtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002734
VERSION     KHUT01002734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggattta ctgggcgtaa agggcgcgta ggcggcttga
       61 taagtcagtc gtgaaagccc ggggctcaac cccggaagtg cgattgaaac tatcaggctt
      121 gagtatggta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg acttcctgga ccaatactga cgctgatgcg cgaaggtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002735
VERSION     KHUT01002735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtcagg
       61 taagtgggat gtgaaatcct ccggctcacc cggagacctg catcccaaac tgtctggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002736
VERSION     KHUT01002736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           48 c           98 g           52 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacac ggggcttaac ttcgtgcttg ctttcgatac gggcagactg
      121 gaggcatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg catgtcctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002737
VERSION     KHUT01002737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           79 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggacagg
       61 taagtctgtg gtgaaatctc cgagcttaac tcggaaactg ccatggatac tctctgtctt
      121 gaatactgtg gaggtgagcg gaacatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactgca cagtgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002738
VERSION     KHUT01002738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcgggc
       61 gaggtcgggg gtgaaatccc acagcttaac tgtggaagag ccttcgatac cggctcgctg
      121 gaggaaggta ggggtgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggc ggcgaaagcg gcgcactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002739
VERSION     KHUT01002739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccaga
       61 caagtagggt gtgaaagccc ccggcttaac cggggaatgg cgccctaaac tgtatggctg
      121 gagtgagata ggggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcacactgga tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002740
VERSION     KHUT01002740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggactgg
       61 caagtcaggg gtgaaatctc acagctcaac tgtgaaactg cctttgatac tgctggtctt
      121 gagtcaggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc cctgaactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002741
VERSION     KHUT01002741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           94 g           51 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagagtgtt gtgaaatctt cgggctcaac tcgaagtctg caacgctgac tgccgtgctt
      121 gagtgtggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002742
VERSION     KHUT01002742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           97 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccgg
       61 taagtcggtt gtgaaaactg ggggctcaac cctcagaggc cgaccgatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002743
VERSION     KHUT01002743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtatt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatttatctt
      121 gaatcttgtt gaggtaggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gcttactaaa caggcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002744
VERSION     KHUT01002744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          106 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctct
       61 taagcgtgtg gtgaaagccc ggggctcaac tccgggtcgg ccgtgcgaac tgggggactg
      121 gagcttgtca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg acagtgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002745
VERSION     KHUT01002745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggttgttcga
       61 taagtctgct gtgaaagtcc aaggctcaac cttgggatgc cggtggatac tgtctgacta
      121 gagtacggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002746
VERSION     KHUT01002746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcag
       61 taagtccgct ctgaaagccc ggggctcaac cccgggaggc gggtggatac tgctgagctc
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gcactctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002747
VERSION     KHUT01002747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           99 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ttgggcgtaa agggcgcgta ggcggcgctg
       61 ttagtcaggc gtgaaatgcc caggctcaac ctggggactg cgcttgatac ggcagggctc
      121 gaggatggaa gagggccgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcggcctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002748
VERSION     KHUT01002748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          105 g           35 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggaccgc
       61 cacgtgccgt gtgacaagcc actgctcaac agtggagggc cagggtacac gagcggtctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggt cctgttctga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01002749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002749
VERSION     KHUT01002749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          105 g           38 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgacg
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgcgttgctt
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002750             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002750
VERSION     KHUT01002750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgagac tgctatgact
      121 agagtctggt aggggaccac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002751             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002751
VERSION     KHUT01002751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcgggcatc
       61 taagtcggtg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tggatgtctt
      121 ggagtccggg agaggtgagt ggaactgcga gtgtagaggt gaaattcgta gatattcgca
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002752
VERSION     KHUT01002752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggctttc
       61 taagtctgat gtgaaatcct ggggctcaac cctggaactg cattggatac tgggaggcta
      121 gagtactgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagttactga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002753
VERSION     KHUT01002753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtttcat gtgaaatctc caagcttaac ttggaaatgg catggaaaac tattcggcta
      121 gaggattgga agggggactg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cgctcaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002754
VERSION     KHUT01002754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           75 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtggattag
       61 taagtcaggg gtgaaatctt caagcttaac ttggaaactg cctttgatac tattagtctt
      121 gaatatcctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg ggattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002755
VERSION     KHUT01002755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcggatcaa
       61 taagtcagag gtgaaatccc gcagctcaac tgcggaactg cccttgatac tgttgatctt
      121 gagtatcgga gaggtcacca gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaatacctga ggcgtaggcg ggtgactgga cgattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002756
VERSION     KHUT01002756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggccagg
       61 taagttggat gtgaaatctc ccggctcaac tgggagagga cgttcaagac tgcctggctt
      121 gaggacggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccgtttctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002757
VERSION     KHUT01002757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           89 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaagcg
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgcgtttctt
      121 gagccaatca ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggcgaaagca gacgactggg attgcgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002758
VERSION     KHUT01002758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           88 g           49 t
ORIGIN      
        1 gacgagctct ccaaacgtta ttcggtatca ctgggcttaa agagttcgta ggcggtctgg
       61 taggtgaggt gtgaaatccc ccggctcaac cggggaactg cgcttcaaac caccggactt
      121 gagggagaca gaggtgggcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gcccactggg tcttttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002759
VERSION     KHUT01002759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           51 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattca ttgggcgtaa agggagcgta ggcggtcggg
       61 taagtcggat gtgaaatcct ggggctcaac ctcagaactg cattcgatac tgcttggcta
      121 gaggactgga gaggtgagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagc ggcgaaggcg gctcactgga cagtttctga cgctgatgct cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002760
VERSION     KHUT01002760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttctt
       61 taagtcaggt gtgaaatccc tgggctcaac ctgggaactg catttgatac tgaagagcta
      121 gagtgtggta gaggatagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctatctgga ccaacactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002761
VERSION     KHUT01002761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           97 g           49 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttgcg
       61 caagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tgcgtggcta
      121 gagtgcggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctctctgga ccgcgactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002762
VERSION     KHUT01002762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagac gtgaaatctc tgggcttaac ccagaaattg cgtttgaaac tggaaggctt
      121 gaggatcgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cgacacctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002763
VERSION     KHUT01002763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 gaagttgggt gtgaaagctc ggggcttaac cccgaaacgg cattcaaaac tcctttgctc
      121 gagggtcgga agggagactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002764
VERSION     KHUT01002764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg cattcgatac tggcaggcta
      121 gagtatggta gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gcttcctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002765
VERSION     KHUT01002765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggatgtg
       61 caggcatgtt gtgaaatccc atggctcaac catggaattg cttcgtgaac cgcacatctt
      121 gaggcaggta ggggcggtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccaac ggagaagtca ggccgctggg cctgtcctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002766
VERSION     KHUT01002766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ctgggcttaa agcgtgcgta ggcggcttgg
       61 taagttgggt gtgaaatcct tcggctcaac cggagaatag cgcccaaaac taccaagctt
      121 gagggagata ggggtgagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002767
VERSION     KHUT01002767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           99 g           39 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggcggcctgc
       61 taagttcggc gtgaaagcct ccggctaaac cgggggaggt cgtcggaaac tggcaagcta
      121 gagggtggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccacacctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002768
VERSION     KHUT01002768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           58 c          109 g           40 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggcttcg
       61 cgcgtccgac gtgaaagccc ccggctcaac cggggagggt cgttgggtac ggcggagctt
      121 gagggcggta gaggttggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccaactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002769
VERSION     KHUT01002769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           97 g           47 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtagtc
       61 caagtctggt gtgaaatctc ccggcttaac cgggagggtg cgccggaaac tgggctgctg
      121 gagtgcggga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggtgtagacg gcttactgga ccgccactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002770
VERSION     KHUT01002770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           94 g           42 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccttt
       61 taagtcctgc gtgaaatcct gcggctcaac cgcagacggt cgtgggaaac tggaaggcta
      121 gagtgcggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002771
VERSION     KHUT01002771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgcgca ggcggcccct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggc cattggaaac tggggggctt
      121 gagtgcagga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccgat ggcgaaggca gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002772
VERSION     KHUT01002772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           85 g           49 t
ORIGIN      
        1 tacgagttgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctatg
       61 aaagtcagat gtgaaatccc tcggctcaac cgaggaactg cacttgaaac tgcatagctt
      121 gagggagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttatctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002773
VERSION     KHUT01002773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggtggtctgt
       61 taagtctggc gtgaaagctc tcggctaaac cgggagaggt cgttggatac tggcggacta
      121 gaggacggta gaggtgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacactggg ccgtacctga cactaagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002774
VERSION     KHUT01002774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           85 g           55 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtttgt
       61 caagtcagtt gtgaaatctc cgagcttaac tcggaacggt caactgatac tgtcaaacta
      121 gagtacagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgatactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002775             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002775
VERSION     KHUT01002775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           92 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgtta tccggaatga ttgggcgtaa agcattcgta ggtggtagat
       61 gaagtctact gtcaaatacc agggctcaac cttggacagg cagtggagta ctcatttact
      121 agagcgtggt agaggtagag ggaattcccc gtggagcggt gaaatgcgta gagatgggga
      181 agaacaccgg tggcgaaagc gctctactgg gccaaggctg acactgaggg atgaaagctg
      241 ggggagcaaa tagg
//
LOCUS       KHUT01002776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002776
VERSION     KHUT01002776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggccaac
       61 taagtcggat gtgaaatccc caggctcaac ctgggaactg catccgatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgagattga cgctatagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002777
VERSION     KHUT01002777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggaatta ttgggcgtaa agagcgcgta ggcggttcgg
       61 taagtctgac gtgaaatccc ggagctcaac tccggaactg cgttggatac tgccgggctt
      121 gagtgtggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccacaactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002778
VERSION     KHUT01002778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           84 g           50 t
ORIGIN      
        1 gacgaaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttaggt gtgaaatccc ccggctcaac cggggaattg cgcttaaaac tactgtgcta
      121 gaggaataca gaggtgagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gctcactggg tattttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002779
VERSION     KHUT01002779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          103 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 taagtcgggg gtgaaacgct ggggctcaac cccagcactg ccttcgagac tgtcaggctt
      121 gagcacggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002780
VERSION     KHUT01002780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          108 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtgggg
       61 taaggctatg gtgaaagccc ggggctcaac cccgggtcgg ccgtggcgac tgcccgactc
      121 gagcacagta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002781
VERSION     KHUT01002781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           88 g           47 t
ORIGIN      
        1 gacgaaccgt acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgctg
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac tacagtgctt
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacgccagg ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002782
VERSION     KHUT01002782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggccgag
       61 caagtcgagt gtgaaagccc cgagcttaac ttgggaactg cgctcgaaac tacttggcta
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002783             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002783
VERSION     KHUT01002783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgtgtgta ggcggccacg
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cacccgatac tgcgacggct
      121 tgagtaccgg agaggttggt ggaattctcg gtgtagaggt gaaattcgta gatatcgaga
      181 ggaacaccgg tggcgaaggc ggccaactgg acgagtactg acgctgagac acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002784             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002784
VERSION     KHUT01002784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           47 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcacgt gtgaaatctc cacgctcaac gtggaggggt cacgtgatac tgccgtggct
      121 agagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002785
VERSION     KHUT01002785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggttcgt
       61 cgcgtcgatt gtgaaaactt ggggctcaac tccaagcttg cagtcgatac gggcgaacta
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgagaag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002786
VERSION     KHUT01002786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggccttt
       61 tcagtcagac gtgaaatccc caggcttaac ctgggaactg cgtctgatac tggaaggctt
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgaaactga cactgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002787
VERSION     KHUT01002787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctgg
       61 taagtcggat gtgaaatccc tcggctcaac cgaggaagtg catccgaaac tgctgggctc
      121 gagtactgaa gagggtcgca gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcgacctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002788
VERSION     KHUT01002788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccaaa
       61 taagtctggg gtgaaagccc gctgctcaac agcggaactg ccctggatac tgtatggctt
      121 gaggacagac gaggttggcg gaatagaggg tgtagcggtg aaatgcatag ataccctcta
      181 gaaccccgat tgcgaaggca gctgactaga ctgtatctga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002789
VERSION     KHUT01002789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgagc
       61 atagtcaggt gtgaaagccc cgggctcaac ccgggaactg cacttgatac tggcttgcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002790
VERSION     KHUT01002790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           83 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggctatg
       61 taagacagat gtgaaatccc tgggcttaac ctaggaactg catttgtgac tgcattgctg
      121 gagtgtggta gagggagatg gaattccgca tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccaat ggcgaaggca atctcctgga cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002791
VERSION     KHUT01002791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          100 g           45 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgtt
       61 tgcgtcggat gtgaaagccc ggggctcaac ctcggaacgg cattcgatac gggacggctg
      121 gagaccggga gaggagagtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002792
VERSION     KHUT01002792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           91 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttcgg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tcccgaactc
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacatcggt ggcgaaggcg gccttctgga cagcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002793
VERSION     KHUT01002793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           75 g           64 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggactta
       61 taagtcagag gtgaaagccg gtagcttaac tatcgaattg cctttgaaac tgtaagtctt
      121 gaattttgtt gaggtaggcg gaatgtatca tgtagcggtg aaatgcatag atatgataca
      181 gaacaccgat tgcgaaggca gcttactaag caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002794
VERSION     KHUT01002794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c          100 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agagcgtgta ggcgggtgtg
       61 caagtttggc gtgaaagctc ccggctaaac tgggagagtg cgctgaagac tgcatgtcta
      121 gaggcaagta gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acgtcctggg cttgacctga cgctgagacg cgacagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002795
VERSION     KHUT01002795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           49 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccttg
       61 taagtcttgg gtgaaatccc tcagctcaac tgaggaactg cctcggatac tgcagggctt
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002796
VERSION     KHUT01002796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccgtg
       61 caagtggaag gtgaaatccc tcggctcaac cgaggaactg cctttcaaac tgcttggctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002797
VERSION     KHUT01002797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 caagtcgggt gtgaaatccc tcagctcaac tgaggaatgg cgcccgaaac tgctgtgctc
      121 gaggaagata gaggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcgtactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002798
VERSION     KHUT01002798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           77 g           57 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ttgggcgtaa agggtgcgca ggcggcttgg
       61 tcagttaggt gtgaaaactc ggagctcaac tccgaaattg catttaatac tactaggcta
      121 gagtgctgta taggaagctg gaacttacgg tgtagcagtg aaatgcgtag atatcgtaat
      181 gaagaccaga ggcgaaagcg agcttctgag cagttactga cgctcatgca cgaaagccaa
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002799
VERSION     KHUT01002799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcatta
       61 taagtcactt gtgaaatctc cgggcttaac tcggagcggc caagtgatac tgtaaagcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002800
VERSION     KHUT01002800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          100 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcggat gtgaaaaccc ggggctcaac cccgggctgt cattcgatac tgccgggctc
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002801
VERSION     KHUT01002801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcgcat
       61 caagttcgac gtgaaagctc ccggctcaac tgggagaggt cgttgaagac tgatgtgctt
      121 gaggcaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc attagcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002802
VERSION     KHUT01002802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           88 g           50 t
ORIGIN      
        1 cacgaaccgg gcaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgcgcta
       61 taagtcgggt gtgaaatccc tcagctcaac tgaggaactg cgcccgatac tgtagtgctt
      121 gaggaggata gaggtgagcg gaactagcag tggagcggtg aaatgcgttg atattgctgg
      181 gaacacccgt ggcgaaagcg gctcactggg tcctttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002803
VERSION     KHUT01002803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          103 g           38 t
ORIGIN      
        1 gacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggtgggtgcg
       61 caagtcggag gtgaaagcgc agggctcaac cctgcgacgc ccttcgagac tgcgtgtcta
      121 gggaccggta gaggtgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccgga ggcgaaggcg gctcactggg ccggatccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002804
VERSION     KHUT01002804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          100 g           44 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggactta ctgggcgtaa agcgtgggta ggtggatggt
       61 gatgtgccgt gtgaaagcgc cgcgcttctc gcggcgaggt cgcggtagac acaccatcta
      121 gaggcacgga gaggggcgta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgcaagcg gcgccctggc cgtgacctga cactgagcca cgacggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002805
VERSION     KHUT01002805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacgagggat cctagcgttg ttcggaatca ttgggcgtaa agagtatgta ggcggcctag
       61 taagtcagtt gtgaaagccc cgggctcaac ccgggaagtg caattgatac tgcttggctt
      121 gaatgtggaa gagggcagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctgcctggt cctacattga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002806
VERSION     KHUT01002806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c          100 g           36 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 caagtcagga gtgaaagccc ggggctcaac cccggaacgg cttttgagac tgccgagcta
      121 gagtgcggaa ggggcaaccg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccgga ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002807
VERSION     KHUT01002807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggctttg
       61 caagttcgac gtgaaagctc tcggcttaac tgggagaggt cgttgaaaac tgcagggctt
      121 gaggcaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc cttagcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002808
VERSION     KHUT01002808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           50 c          103 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgt
       61 tgcgtctgcc gtgaaatccc ggggcttaac tccgggtctg cggtggatac gggcggactg
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caggtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002809
VERSION     KHUT01002809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttata
       61 taagacagat gtgaaatccc cgggctcaac ctgggaactg cgtttgtgac tgtatgacta
      121 gagtacggta gagaggggtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatcgat ggcgaaggca gccccttgga cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002810             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002810
VERSION     KHUT01002810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           77 g           59 t
ORIGIN      
        1 tacgaagggt gtaagcgttg cccggattta ttgggcgtaa agtgtttcgt aggcgtcctg
       61 ataagtcatt ctttaaagct tcccgctcaa cgggaaaaaa gggaatgata ctatcaggat
      121 tgaagaatgt tggagttact ggaactatcg gtgtaggggt gaaatccgtt gatatcgata
      181 ggaactccga aagcgaaggc gagtaactaa acaaatcttg acgctgagga acgaaagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01002811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002811
VERSION     KHUT01002811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           59 c          111 g           37 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggcttca
       61 cgcgcccgac gtgaaagccc ccggcttaac cggggagggt cgtcggggac ggtggagctt
      121 gagggcggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccgactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002812
VERSION     KHUT01002812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           88 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcaatg
       61 taagtctgct gtcaaagcgt ctggctcaac cagataaagg cagtggaaac tacatagcta
      121 gagtaaggta ggggtagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctgga ccttaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01002813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002813
VERSION     KHUT01002813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           48 c           98 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggttcgt
       61 taagtttgag gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcggactt
      121 gagggaggca gaggaaagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccttacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002814
VERSION     KHUT01002814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          107 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttcgc
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tggcggactt
      121 gagcactgta gaggcgggta gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaataccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002815
VERSION     KHUT01002815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttttt
       61 caagtcagag gtgaaagccc ggggctcaac cccggaatag cctttgaaac tggacgacta
      121 gaatcacgga gaggtgggtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcccactgga cgtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002816
VERSION     KHUT01002816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 taagtttgac gtgaaatccc cgggcttaac ctgggaatgg cgttgaagac tgcacggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002817
VERSION     KHUT01002817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatttc
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cccttgatac tgggaatctc
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002818
VERSION     KHUT01002818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaccgg
       61 taagtcgggt gtgaaatccc tgggctcaac ccaggaactg catccgaaac tactggcctt
      121 gagtgtcgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002819
VERSION     KHUT01002819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           93 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgca
       61 taagtcgaat gtgaaatccc ccggctcaac tggggagcgt cattcgatac tgtgcgactc
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002820
VERSION     KHUT01002820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 aacgtaggag gcgaacgtta tccggattta ctgggcgtaa agcgcatgca ggcggtttct
       61 taagtcggac gtgaaagctc ctggctcaac tgggagaggt cgttcgaaac tgggaaactt
      121 gaggatggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccattcctga cgctaagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01002821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002821
VERSION     KHUT01002821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           88 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgcccgtctt
      121 gagccactta ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gacgactggg agtgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002822
VERSION     KHUT01002822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttctt
       61 taagtcgggt gtgaaatctc agggctcaac tctgaaactg cacccgatac tggagaactt
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002823             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002823
VERSION     KHUT01002823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           64 c           98 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggaggg
       61 cacgtccgtc gctgaaatcc cccggctcaa ccggggaacg ggcgcggata cgaccttcct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002824
VERSION     KHUT01002824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ctgggcgtaa agggcgagca ggtggcacta
       61 caagtctgat gtaaaatctc ccggcttaac tgggaggggc cattggaaac tgtagagctg
      121 gagtgcagga gaggctaacg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttagctggc ctgtaactga cactgaggcg cgaaggctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002825
VERSION     KHUT01002825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtgcga
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tgtcggactg
      121 gagcgtggta ggggaaggcg gaattccagg tgtagtggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccacgactga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002826
VERSION     KHUT01002826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 cacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttttg
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tgcaagacta
      121 gagcatggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaagcg gccttctggg ccatgtctga cgctcagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002827
VERSION     KHUT01002827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggcttgg
       61 tgagtcggac gtgaaagctc ctggcttaac tgggagaggt cgttcgagac tgccgggctt
      121 gaggttggga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002828
VERSION     KHUT01002828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgag
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaattg cactcgatac tgcttagcta
      121 gagtgtggaa gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctggt ccaacactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002829
VERSION     KHUT01002829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          105 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtccgg
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcgaac tgccggactc
      121 gagcactgta gaggcaggtg gaattccgag tgtagcggtg gaatgcgtag agattcggag
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtgtctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002830
VERSION     KHUT01002830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatatt
       61 ttagtcaggg gtgaaatccc ggggctcaac cccggaactg cctctgatac tggatatctt
      121 gagttcgaga gaggtaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccaga ggcgaaggcg gcttactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002831             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002831
VERSION     KHUT01002831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           99 g           48 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggccttg
       61 taagtctgac tgtgaaagtc cggggctcaa ccccgggatt gcaggggata ctgcgaggct
      121 tgagacaagt agaggttggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggccaactgg gcttgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002832
VERSION     KHUT01002832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taagttaggg gtgaaatccc ggagctcaac tccggaactg cctctaagac tgcatcgctt
      121 gaatccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002833
VERSION     KHUT01002833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggcggattag
       61 taagtcagag gtgaaacccc gaagcttaac ttcggaactg cctttgatac tgctagtcta
      121 gagtttggga gaggcgagtg gaatgtctgg tgtagcagtg aaatgcgtag atatcagaca
      181 gaacaccaat ggcgaaggca gctcgctggt ccaatactga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002834
VERSION     KHUT01002834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacggagggg gcgagtgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcttgg
       61 caagtcaagt gtgaaatccc tcagcttaac tgaggaattg cgcttgaaac tacctcgcta
      121 gagggtggga gagggaagtg gaactctcgg tgtaggggtg aaatctgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctggc ccacatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002835
VERSION     KHUT01002835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           85 g           56 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattta ctgggcgtaa agggtgtgta gacggtttac
       61 taagtctgtt gttaaatact ctggcttaac tggagatcag cagtggaaac tagtagacta
      121 gaggttggga gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccgat ggcgaaggca gcttcttggc ccatacctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002836
VERSION     KHUT01002836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggctgc
       61 caagtttgag gtgaaatcct ccggctcaac cggggaagtg ccttgaatac tggcggtctg
      121 gagggaggta ggggcgtgcg gaactaacgg tggagcggtg aaatgcgttg atatcgttag
      181 gaactccggt ggcgaaggcg gcacgctaga cctcttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002837
VERSION     KHUT01002837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          101 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agggcacgca ggcggggtga
       61 tgcgtttccg gtgccagcct ctcgcttaac gaggggagtg ccgggaagac gatcactctt
      121 gagggcctca gagggacata gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggg aggcaactga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002838
VERSION     KHUT01002838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcca
       61 tcagtctgtg gtgaaatcct ccagcttaac tggagaagtg ccatggatac tgtgggtctc
      121 gaggaggata ggggcatctg gaactgccgg tggagcggtg aaatgcgttg atatcggcag
      181 gaactccggt ggcgaaggcg agatgctgga tcctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002839
VERSION     KHUT01002839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           92 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgtacgca ggctgttcgt
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaacgg cgcccgaaac tggcgagctt
      121 gaatgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacattga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002840
VERSION     KHUT01002840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcggag gtgaaatccc agggctcaac cctggaactg ccttcgatac tggctgtctc
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002841
VERSION     KHUT01002841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcttga
       61 caagtcactt gtgaaatctc tgggcttaac ccagaacggc caagtgaaac tgtcgagcta
      121 gagtgtagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggt ctaacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002842
VERSION     KHUT01002842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgcgta ggcggttact
       61 taagttggat gtgaaagccc tcggcttaac tgaggaagtg catccaaaac tgagtagcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002843
VERSION     KHUT01002843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggttcgt
       61 taagtcagtt gtgaaagccc ggagctcaac tccggagagt cgactgatac tggcgagcta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg ggctactggg ccgatactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002844
VERSION     KHUT01002844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           79 g           60 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacttt
       61 caagtcagtg gtgaaagccc atcgctcaac gatggaattg ccattgatac tgttggtctt
      121 gagtacggtt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacacctat tgcgaaggca gctcgctaag ccgtaactga cgctgaggca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002845
VERSION     KHUT01002845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacgtactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctttt
       61 caggtctggt gtgaaatccc ccggctcaac cggggaatgg cattggaaac cgaaaggctt
      121 gaggtagcta gaggcatgcg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg gcatgctggg gctattctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002846
VERSION     KHUT01002846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           79 g           57 t
ORIGIN      
        1 tacgtaggat cctagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactta
       61 taagtcagtg gtgaaatctc ggcgctcaac gccgaaactg cctttgatac tataagtctt
      121 gagtttggta gagatccgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagc ggcgaaggca gcggattggg ccaatactga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01002847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002847
VERSION     KHUT01002847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcacgca ggtggcttgg
       61 caagtcggat gtgaaagccc cgggcttaac ttgggaattg cattcgatac tgccgagcta
      121 gagtatggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctttctggc ccaatactga cactcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002848
VERSION     KHUT01002848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggggac
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaactg ccttggatac tggtcttctt
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002849
VERSION     KHUT01002849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 gacgaaggtg gcaagcgtta ttcggattta ctgggcataa agcgcgcgta ggcggcttgg
       61 caagtcgggg gtgaaagccc gcagctcaac tgcggaactg ccttcgatac tgccgggctt
      121 gagggcggga ggggtgattg gaactccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgga ggcgaaggcg aatcactgga ccgcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002850
VERSION     KHUT01002850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggactga
       61 caagtacttt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgtcggtctt
      121 gaggcatcta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg gatgtactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002851
VERSION     KHUT01002851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           91 g           48 t
ORIGIN      
        1 gacggaggtc cctagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggggtga
       61 caagtacctt gtgaaatccc cgggctcaac ccgggaatgg ctcggtatac tgtcactctt
      121 gaggcaggta gaggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgccctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01002852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002852
VERSION     KHUT01002852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacgtatggg gcaagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggattgt
       61 taagtcagat gtgaaaactg ggggctcaac ccgcagcctg catctgaaac tggcagtctt
      121 gagtctggta gaggagtgtg gaattcccag tgtagcggtg aaatgcgcag atattgggag
      181 gaacaccagt ggcgaaggcg gcactctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002853
VERSION     KHUT01002853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           73 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggccttg
       61 taagtcagtg gtgaaatctt gcggctcaac cgtaaaattg ccattgatac tgcagggctt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact aatatattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002854
VERSION     KHUT01002854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          100 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtgtgt
       61 cgcgtctgcc gtgaaaacct gcggcttaac cgtgggcgtg cggtggatac gggcacactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg ggtctctggg ccgaaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002855
VERSION     KHUT01002855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctctt
       61 caagtcggac gtgaaagccc tgggcttaac ccaggaactg cgctcgatac tgtagagcta
      121 gagtgtggaa ggggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccaacactga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002856
VERSION     KHUT01002856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           92 g           48 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcggtg gtgaaatcct acagcttaac tgtagaactg cctccgatac tggttgtctc
      121 gaggaaggta ggggcgtgcg gaacagttgg tggagcggtg aaatgcgttg atatcaactg
      181 gaactccggt ggcgaaggcg gcacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002857
VERSION     KHUT01002857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggattta ctgggcgtaa agagtctgta ggcggtgaag
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgcttcactt
      121 gagggtcgga ggggagtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacactagt ggcgaaggcg agactctgga cgacaactga cgctgagaga cgaaggccag
      241 gggagcgaaa agg
//
LOCUS       KHUT01002858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002858
VERSION     KHUT01002858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggcttgta ggcggtccgt
       61 taagtccgat gtgaaatctc ccggctcaac tgggagaggt cattggatac tggcggacta
      121 gaggggggta gaggaaagca gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccagt ggcgaaggcg gctttctggg cctaccctga cgctgaggag cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002859
VERSION     KHUT01002859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           82 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcgcgca ggcggctttg
       61 taagttggat gtgaaatcct atggctcaac catagaactg catccaaaac tacttagcta
      121 gagtctggga ggggaagatg gaattagtgg tgtaggggta aaatccgtag agatcactag
      181 gaataccaaa agcgaaggcg atcttctgga acagtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002860             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002860
VERSION     KHUT01002860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           44 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagctcgta ggcggttcgg
       61 caagtcgggt gtgacaactc caggctcaac ctggagaggt cactcgatac tgctgtgact
      121 agagtccggt aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002861
VERSION     KHUT01002861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 gacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgcgg
       61 taagtcggaa gtgaaatctc tgggcttaac tcagaggctg ctttcgaaac tgccgtgctg
      121 gagtgcggga gaggcgagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctcgctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002862
VERSION     KHUT01002862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 cacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 tgcgtcgaaa gtgaaaatcc ggggcttaac tccggacttg ctttcgatac gggcagacta
      121 gaggtaggca ggggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002863
VERSION     KHUT01002863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           92 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtcattc gtgaaaactt ggggctcaac ttcaagcttg cgggcgatac gggcagacta
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgagaag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002864
VERSION     KHUT01002864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtcgggt gtgaaatcct ggggctcaac cccaggactg cactcgatac tgccaggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002865
VERSION     KHUT01002865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacagag gtgaaatccc cgggctcaac ctgggaactg cctttgtgac tgcaaggctt
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002866
VERSION     KHUT01002866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           99 g           38 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgc
       61 caagtcccgt gtgaaagccc ccggctcaac cggggaacgg cgcgggaaac tagcaggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgcag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002867
VERSION     KHUT01002867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggcggtccac
       61 taagtcatat gtgaaagccc tcagctcaac tgaggaacga cgtatgaaac tgatggactc
      121 gagtacggga gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggt ccgatactga cgctaatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002868
VERSION     KHUT01002868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           97 g           50 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcaggc gtgaaagccc tgggctcaac ctgggatgtg cgtttgatac tggcaagctt
      121 gagtgcagga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002869
VERSION     KHUT01002869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           89 g           44 t
ORIGIN      
        1 tacgtagggt ccaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtctcg
       61 caagtccgat gtgaaatccc cgggcttaac ctgggaactg cgttggaaac tacgaggcta
      121 gagtgcggca gaggggggtg gaattccagg tgtagcagtg aaatgcgtag agatctggag
      181 gaacaccgat ggcgaaggca gccccctggg ctagcactga cgctcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002870
VERSION     KHUT01002870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacgaaggtc ccgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgtgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccgtgcta
      121 gaggattgga gaggtagctg gaattctcgg tgtagcagtg aaatgcgtgg agatcgagag
      181 gaacactcgt ggcgaaagcg agctactgga caatatctga cgctgaggct cgaaggccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01002871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002871
VERSION     KHUT01002871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggactgt
       61 taagtcgggg gtgaaatcct ggggctcaac cccagaattg cctccgatac tggcagtctc
      121 gagtgcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgtaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002872
VERSION     KHUT01002872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggccttc
       61 ttagtcagac gtgaaatccc cgggcttaac tcgggaactg cgtctgatac tggaaggctc
      121 gagttcggga gaggaatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccgaaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002873
VERSION     KHUT01002873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           75 g           61 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggacttt
       61 taagtcagtg gtgaaagctc gcagcttaac tgtgaaattg ccattgaaac tgaaagtctc
      121 gagtgtggtt gaggtaactg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca ggttactgag ccacaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002874
VERSION     KHUT01002874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcgggtctt
       61 taagtcaggg gtgaaatgcc aaggctcaac cttggaactg cctttgatac tggagatctt
      121 gagtccggga gaggtgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002875
VERSION     KHUT01002875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcttgt
       61 taagtagggt gtgaaatccc ccggcccaac cggggaattg cgctctaaac tagcaggctt
      121 gagtgagcta ggggtgcacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgcactggg gcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002876
VERSION     KHUT01002876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           64 c          109 g           30 t
ORIGIN      
        1 gacgtaggag acgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggccccg
       61 caggtcggtt gtgaaagccc ccggctcaac cgggcggagg cgagcgaaac cgcggggctg
      121 gaggctggca gagggtggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgc ggcgaaggcg gccacctgga ccaggcctga cgctgaggcg cgaaggccgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002877
VERSION     KHUT01002877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           88 g           50 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtaga gtgaaatctc tgggctcacc ccagaaactg ctctgcatac tggcgagctg
      121 gagggatgaa gaggcttgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcaagctggt catcacctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002878
VERSION     KHUT01002878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtgacc
       61 taagtcagtt gtgaaatact tcagcttaac tggagaggtg caattgatac tgggttactt
      121 gagtactgat gaggtatgcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcatactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002879
VERSION     KHUT01002879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtccgta ggtggccaac
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggtcggctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgaacga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002880
VERSION     KHUT01002880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatta ctgggcttga agcgcgcgta ggcggccccg
       61 caagtgcggt gtgaaatccc acggcttacc cgtggaaccg cgccgtgtac tacggggcta
      121 gaggatggta gaggagggcg gaactcttgg tggagcggtg aaatgcgtag agatcaagag
      181 gaacaccggt ggcgaaggcg gccctctggt ccattactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002881
VERSION     KHUT01002881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcctcg
       61 taagtcagtc gtgaaagccc ggggctcaac cccggagggt cgactgatac tgcgaggcta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002882
VERSION     KHUT01002882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcccac
       61 taagttaggt gtgaaatccc ccggctcaac cggggaacgg catctgatac tggtgggctc
      121 gaggacggta ggggtaagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactggg ccgtgtctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002883
VERSION     KHUT01002883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcagaatta ctgggcgtaa agggcgcgta ggcggttata
       61 taagttagat gtgaaatccc tgggcttaac ctaggaactg catctaatac tgtatagcta
      121 gagtgcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002884
VERSION     KHUT01002884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           86 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ctcggaatca ctgggcttac agggtgcgta ggtggcttga
       61 taagcagggt gtgaaatccc tcggctcaac cgaggaactg cgctctgaac tgtcgggcta
      121 gagtatgcta gaggtgaatg gaacatccag tggagcggtg aaatgtgttg atattggatg
      181 gaacaccaga ggcgaaggcg attcactggg gcataactga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002885
VERSION     KHUT01002885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccat
       61 taagttcggt gtgaaatccc ccggctcacc cggggaattg cgccggaaac tggtggactc
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002886
VERSION     KHUT01002886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           78 g           58 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggacttg
       61 ttagtcagag gtgaaatctc actgctcaac agtgaaactg cctttgatac tgcaggtctt
      121 gaatccagaa gagagttgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagc ggcgaaggca gcaacttggt ctggtattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01002887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002887
VERSION     KHUT01002887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagacagat gtgaaatccc cgggcttaac ctgggaactg cgtttgtaac tggcaagcta
      121 gagtgtggca gaggggggtg gaattccaag tgtagcagtg aaatgcgtag atatttggag
      181 gaacaccaat ggcgaaggca gccccctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002888
VERSION     KHUT01002888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtgggctgg
       61 taagttcgat gtgaaagccc cgggcttaac ccgggaatgg cattggatac tactggtctg
      121 gaggatcgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgatacctga cactgaggca cgaaagccaa
      241 gggagcaaac agg
//
LOCUS       KHUT01002889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002889
VERSION     KHUT01002889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           74 g           65 t
ORIGIN      
        1 tacgaagggt ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggttgt
       61 taagtcagtg gtgaaatctt gcagcttaac tgtaaaattg ccattgatac tgacgatctt
      121 gagtacactt gaagtgggcg gaatatgtca tgtagaggtg aaattcttag agatgacata
      181 gaacaccgat tgcgaaggca gctcactaaa ttgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002890
VERSION     KHUT01002890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           42 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggattca ctgggcataa agggcgcgta ggcggttccg
       61 aaagtggcat gtgaaagccc ccggctcaac cggggaagtg cgtgccaaac ttcggtccta
      121 gagggtggga ggggggactg gaactctcgg tggagcggtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctggt ccacttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002891
VERSION     KHUT01002891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          100 g           39 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtttgg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggccggactg
      121 gagggctgca gaggtcgatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtcgactggg cagcacctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002892
VERSION     KHUT01002892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           83 g           56 t
ORIGIN      
        1 gacgaaccat gcaaacgtta ttcggattca ctgggcttaa agcgtgcgta ggcggtcttg
       61 ttagtcaggt gtgaaagccc tcagcctaac tgaggaattg cgcttgatac tgcatgacta
      121 gagaatgata gaggtgggtg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaggcg acccactggg tcatttctga cgctgaggta cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002893
VERSION     KHUT01002893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggctttg
       61 caagtcgatt gtgaaatccc cgggcttaac ctgggaactg cagtcgagac tgctttgctt
      121 gagtatggga gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acctcctgga ccaatactga cactcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002894
VERSION     KHUT01002894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggttcgg
       61 caagtctgat gtgaaagccc agggcttaac cctgggactg cattggaaac tgtcggacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002895
VERSION     KHUT01002895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttt
       61 taagacaggc gtgaaatccc cgggctcaac ctgggaactg cgcttgtgac tggaaggcta
      121 gagtacggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg tcgatactga cgctcatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002896
VERSION     KHUT01002896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           42 c           93 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttgga
       61 taagagtggt gtgaaatctc ccggcttaac tgggagggtg cgctacagac tgtctgacta
      121 gagtatggga tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctctctgga ccattactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002897
VERSION     KHUT01002897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           40 c           72 g           58 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggttgttcaa
       61 taagtcaact gttaaatctt gaggctcaac ttcaaaatcg cagtcgaaac tattaaacta
      121 gagtatagta ggggtaaagg gaatttccag tggagcggtg aaatgcgtag agattggaaa
      181 gaacaccaat ggcgaaagca ctttactggg ctattactga cactgagaga cgaaagctag
      241 ggtagcaaat ggg
//
LOCUS       KHUT01002898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002898
VERSION     KHUT01002898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgcca
       61 taagtcaggt gtgaaatctc ggggctcaac ctcgaaactg cacttgatac tgtggtgctg
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002899
VERSION     KHUT01002899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          100 g           39 t
ORIGIN      
        1 gacgtaggga ccgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccgcg
       61 caagtcgggt gtgaaatccc ggggctcaac cccgggactg catccgatac tgcgtggctc
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01002900             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002900
VERSION     KHUT01002900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           45 c           72 g           62 t
ORIGIN      
        1 tacagagact ccaagcgtta tccggtttca ttgggcgtaa agcgttccga tcggtggttt
       61 agtgtgtcta aggtgaaaga ccgcagctta actgcgtgtt tgctttagaa actactttac
      121 tagaagagta aagaggtagt tggaactcta caagtagggg tgaaatccat tgatatgtag
      181 gggaacacca aaagcgaagg cagactacta agtacttctt gacactgcta ggacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01002901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002901
VERSION     KHUT01002901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggtg
       61 caagtccgat gtgaaatacc ggggctcaac cccggaactg cattggatac tgcattgcta
      121 gagtactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002902
VERSION     KHUT01002902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatatc gcagctcaac tgtgagggtg ccattgatac tgcagggctt
      121 gagtgaattc gaagtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg cttcaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002903
VERSION     KHUT01002903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcaag
       61 taagtggaat gtgaaagccc ccggctcacc cggggatctg catcccaaac tgcttggctt
      121 gagtacggga gaggataggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cctatctggc ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002904
VERSION     KHUT01002904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctttg
       61 caagtcaggt gtgaaagccc tgggctcaac ctaggaagtg cacttgaaac tgcggagctc
      121 gaatgctgga gagggaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagtgattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002905             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002905
VERSION     KHUT01002905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcatg
       61 taagtctggt gtttaatccc ggggctcaac cccggatcgc actggaaact gtgtgacttg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01002906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002906
VERSION     KHUT01002906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           88 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaaag
       61 taagtcgggc gtgaaatccc taggctcaac ctaggaactg cactcgaaac tactatcctt
      121 gagtgccgga gaggaaagcg gaatacctgg tgtagaggtg aaattcgtag atatcaggtg
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002907
VERSION     KHUT01002907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtctga
       61 aaagtcggat gtgaaagccc cgggctcaac ccgggaagtg cattcgaaac tgccaggcta
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaacactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002908
VERSION     KHUT01002908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          101 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggatgtt
       61 taagtgtggc ctgaaagccc ccggctcaac cggggatccg ggttgcaaac tgggcatctt
      121 gagggggata gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg tccgccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002909
VERSION     KHUT01002909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           90 g           45 t
ORIGIN      
        1 tacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggcgatctt
      121 gagccaccta ggggcctgcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca gcaggctggg ggtgtgctga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002910
VERSION     KHUT01002910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgtcc gtgaaaactt ggggctcaac cccaagcttg cgggcgatac gggcagactt
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002911
VERSION     KHUT01002911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           92 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgct
       61 caagtccgtg gtgaaatcct ccggctcaac cggagaactg cctcggatac tgggcgtctc
      121 gagtgtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccaccactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002912
VERSION     KHUT01002912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           81 g           52 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggttgtttaa
       61 aaagtattct gtcaaagatt agggcttaac cctagatagg cagaataaac ttttaagcta
      121 gagtttggta gaggcagagg gaattcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacaccaaa ggcgaaggca ctctgctggg ccaatactaa cactgagaga cgaaagcgag
      241 gggagcaaat ggg
//
LOCUS       KHUT01002913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002913
VERSION     KHUT01002913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccgttgatac tatctgtctt
      121 gaatatcgtt gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaac cgatcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002914             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002914
VERSION     KHUT01002914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           94 g           55 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtccgta ggtggttgtg
       61 taagtctggt gtgaaatctt cgccgcttaa cgggaagcag ccactggaaa ctgcgcaact
      121 tgagtctggt agaggtaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatctg tggcgaaggc ggcttactgg gccagtactg acactgaggg acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01002915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002915
VERSION     KHUT01002915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           76 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggatctt
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tggagatctt
      121 gagtgccgga gaggaaaacg gaacaagttg tgtagcggtg aaatgcgttg atataacttg
      181 gaacaccaat ggcgaaagca gttttctgga cggatactga cgctcatgtt cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01002916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002916
VERSION     KHUT01002916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          103 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggccgcg
       61 tatgtgaccg gtgacatcct cgggctcaac ccgaggcggc ccggttagac agcgtagctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgtactga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002917             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002917
VERSION     KHUT01002917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           38 c           77 g           59 t
ORIGIN      
        1 gacataggtt gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggcatga
       61 caagttatca gtgggaaatc gaggctcaac ctcgtggaag ttggtaatac tattatgctt
      121 gagtgcagga ggagttaacg gaactccatg tggagcggta aaatgtgacg atatatggaa
      181 gaacaccaat aaggcgaaag cagttaacta ttctgttact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01002918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002918
VERSION     KHUT01002918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           86 g           53 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggattta ctgggcgtaa agagctcgta ggcggttgat
       61 caagttggat gtgaaagccc agggcttaac cttggaactg cattcaaaac tagttgactc
      121 gagtaaggga gaggagagtg gaatttccag tgtagcggtg aaatgcatag atattggaag
      181 gaacaccagt ggcgaaggcg actctctggc ctattactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002919
VERSION     KHUT01002919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           85 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggatacg
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg catttgaaac tgcgaatcta
      121 gagtatggta gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttactgga ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002920
VERSION     KHUT01002920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctgt
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg catacgaaac tggcgggctt
      121 gaatatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002921
VERSION     KHUT01002921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcaggg gtgaaatctt tcagctcaac tggaaaacag cttctgatac tggccatctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002922
VERSION     KHUT01002922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           87 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttg
       61 taagttagat gtgaaattcc tgggcttaac ctggggactg cgtctgatac tgcgaagcta
      121 gagtactgta gagggaagtg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002923
VERSION     KHUT01002923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttttc
       61 caagtgagat gtgaaatacc cgggcttaac ctgggaacgg catcttaaac tgggaggctg
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002924
VERSION     KHUT01002924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggcttgt
       61 taagtcttct gtgaaagcta cgggctcagc ccgtaaagtg cagaggaaac tggcgagctt
      121 gagtgtcgga gaggataggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002925
VERSION     KHUT01002925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           98 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttgga
       61 taagttggtg gtgaaaacct cgagcttaac tcggggcctg ccatcaatac tgtctggctg
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccacaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01002926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002926
VERSION     KHUT01002926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           95 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcatgg
       61 caagtcggga gtgaaatccc ggggctcaac cccggaactg ctttcgaaac tgccaagcta
      121 gagtacggaa ggggcaactg gaattccaag tgtagcggtg aaatgcgtag atatttggaa
      181 gaacaccgga ggcgaaggcg ggttgctggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002927
VERSION     KHUT01002927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtccgat gttaaagacc gaggctcaac ctcgatacgg cgttggatac tgacaagctg
      121 gacggttgga gaggaaggta gaattaccag tgtagcggtg gaatgcgtag atactggtag
      181 gaatacccat tgcgaaggca gccttctgga caacacgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002928
VERSION     KHUT01002928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           85 g           47 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggtggccaag
       61 tgtgttaggc gtgaaagccc ggggcttaac cccggaattg cacctaaaac tacttggcta
      121 gagcattgga gaggggagca gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaataccaga ggcgaaggcg gctccctgga caattgctga cactcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01002929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002929
VERSION     KHUT01002929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           92 g           44 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccga
       61 taagtgggga gtgaaagccc tcggcttaac cgaggaagtg ctttccaaac tgtcggactt
      121 gagtacccaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gcgatctggg gggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002930
VERSION     KHUT01002930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          102 g           41 t
ORIGIN      
        1 gacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggcttgt
       61 caagcccgtg gtgaaaggtc ccggcctaac cggggacgtg cttcggggac tgacgagctt
      121 gggcgagcta ggggtctgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acagactggg gcttggccga cgctcaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002931
VERSION     KHUT01002931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           89 g           49 t
ORIGIN      
        1 tacctgcagc ccgagtggtg ctcgttatta ttgggtctaa aacgtccgta gtcggctgag
       61 tagatgcccg ggtaaatcgc agcgcttaac gttgcgaatt ccgggtagac cgctcggcta
      121 gggagtggga gaggtgcgag gtactctgag ggtaggggta aaatcctgta atccttgggg
      181 gaccaccagt ggcgaaggcg tcgcactaga acacgtccga cgatcaggga cgaagccctg
      241 gggcgcaaac ggg
//
LOCUS       KHUT01002932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002932
VERSION     KHUT01002932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           88 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccccg
       61 caagtccgtg gtgaaagcct cttgcttaac aagagaactg ccatggatac tacggggctt
      121 gagtccagac gaggttggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002933
VERSION     KHUT01002933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           88 g           47 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattta ctgggtgtaa agcgtgtgca ggcggcaaag
       61 caagttggat gtaaaatctc ccggctcaac tgggaggtga cgttcaaaac tgcttagctc
      121 gagggcggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctggg ccgtacctga cgctcagaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002934
VERSION     KHUT01002934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttttc
       61 caagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tggaagactg
      121 gagtgtggga gaggcaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt gatgtagatg gcctgctgga ccacaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002935
VERSION     KHUT01002935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c          102 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggtcgtg
       61 tatgtgagcg gtgacatccg cgggctcaac ccgcggcggc ccgcttacac agcgcgacta
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgaactga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002936
VERSION     KHUT01002936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggttcga
       61 taagtctgct gtgaaagtcc agggctcaac cctgggatgc cggtggatac tgtcggacta
      121 gagtgcggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002937
VERSION     KHUT01002937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgact gtgaaaaccc gcggcttaac cgcgggcttg cagttgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002938             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002938
VERSION     KHUT01002938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           94 g           51 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgg
       61 taagtcgggt gtgaaaactc tgggctcaac tcagagacgc cacctgatac tgccgtgact
      121 tgagtctggt aggggagtgt ggaatttccg gtgtagcggt gaaatgcgca gatatcggaa
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtactg acactgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01002939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002939
VERSION     KHUT01002939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaaga
       61 taagtcaggt gtgaaatccc caggctcaac ctgggaactg cacttgaaac tgtctttctt
      121 gagtgtcgga gaggaaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002940
VERSION     KHUT01002940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          112 g           31 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggcgtg
       61 taagtcggga gtgaaagacc ggggctcaac cccggggcgg cttccgagac tgcgggcctg
      121 gggaccggta gaggcgggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002941
VERSION     KHUT01002941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           90 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcgagg
       61 taagtctggt gtgaaatcta ggagctcaac tcctaaactg cactggatac tgctttgctt
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggcg cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002942
VERSION     KHUT01002942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctcat gtgaaatccc gcagcttaac tgcggaacgg catgggatac tattcggctc
      121 gaggattgga ggggggactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cgctgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01002943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002943
VERSION     KHUT01002943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 taagtcagat gtgaaagccc cgggctcaac ccgggaatag catttgatac tgcgaggctt
      121 gagtccggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002944
VERSION     KHUT01002944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcaaggcta
      121 gagtacggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gccccctggg tcgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002945
VERSION     KHUT01002945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           86 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 taagacagat gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcacggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccaat ggcgaaggca gccccctggg acgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002946
VERSION     KHUT01002946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           96 g           46 t
ORIGIN      
        1 aacgtaggac acgagcgtta tccggagtta ctgggcgtaa agggcgttta ggcgggtgac
       61 taagttcgct atgaaagcgc cgggctaaac ccggcgaggc tggcggatac tggtgatctg
      121 gagtgaggta gagggaagta gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaataccagt ggcgaaggcg acttcctgga ccttaactga cgctgaagcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002947
VERSION     KHUT01002947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggatgtc
       61 taagtcgtgt gttaaagccc ctggctcaac tgggggaggt catgcgagac tggatgactt
      121 gagggcaaca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gcgtgctggg ttgcaactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002948
VERSION     KHUT01002948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           87 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggccctg
       61 caagtccgtg gtgaaagcct gctgctcaac agcagaactg ccatggatac tacagggctt
      121 gagtccagac gaggttggcg gaatggaaga tgtagcggtg aaatgcatag atatcttcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002949
VERSION     KHUT01002949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           88 g           54 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agagtccgta ggtgggtaac
       61 caagtcagtt gttaaatagt gaagcttaac ttcatagagg cgactgaaac tggttgtcta
      121 gagtgtggta ggggtagagg gaatttccag tgtagcggtg aaatgcgtag atattggaaa
      181 gaacaccggt ggcgaaagcg ctctactggg cctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01002950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002950
VERSION     KHUT01002950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          101 g           43 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggactta ctgggcgtaa agcgtgggta ggtggccact
       61 gatgtgccgt gtgaaagcgc cgcgcttctc gcggcgaggt cgcggtagac acagtggctc
      121 gaggcacgga gaggggcgta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgcaagcg gcgccctggc cgtgacctga cactgagcca cgacggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002951
VERSION     KHUT01002951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           88 g           55 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggttagg
       61 taagtttgat gtgaaatcct ggggctcaac cctggaactg cattgaatac tgcttgacta
      121 gagtactgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002952
VERSION     KHUT01002952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           75 g           62 t
ORIGIN      
        1 tacgaaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaagccc caagcttaac ttgggaattg ccattgatac tgattgtctt
      121 gagtacactt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ttgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002953
VERSION     KHUT01002953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgttctg
       61 caagtcgggt gtgaaagccc cgggctcaac ctgggaagtg cactcgaaac tgcagaactc
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002954             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002954
VERSION     KHUT01002954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           54 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggaatta ctgggcgtaa agcgtacgta ggtggagatt
       61 taagtgagat gcgaaatctg gcggctcaac cgtctagact gtgtctcata ctgggtttct
      121 tgaggttggc agaggcaagt ggaatttccg gtgtagcagt gaaatgcgta gagatcggaa
      181 ggaacaccaa tggcgaaggc agcttgctgg gccacacctg acactgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01002955             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002955
VERSION     KHUT01002955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggtgggacgg
       61 cacgtcggtc gttgaaatcc cccggctcaa ccggggaagg ggcgccgata cgaccgtcct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttttctg acactgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01002956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002956
VERSION     KHUT01002956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 gacgtagggg gcaagcgttg ttcggaatta ttgggcgtaa agcgctcgta ggcgggattg
       61 gaagtccgtg ttgaaagacc tcggctcaac tgagggaccg gctcggatac tctaattctt
      121 gaggcaatca gaggatgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcatctggg gttgttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002957
VERSION     KHUT01002957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           95 g           45 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatctga
       61 caagtcggct gtgaaagccc agagcttaac tttggaagag cagctgagac tgttggaatt
      121 gagtacagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgtgactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002958
VERSION     KHUT01002958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggcctcg
       61 caagtcgagt gtgaaatccc cgagcttaac ttgggaattg cgctcgaaac tacagggcta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg tcaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002959
VERSION     KHUT01002959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtacgca ggcggtttgc
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgttac tggcaagcta
      121 gagtgtggca gaggggggta gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaataccgat ggcgaaggca gccccctggg ttaacactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002960
VERSION     KHUT01002960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           92 g           44 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaacgg ctgggcatac tggcgatctt
      121 gagcagccta ggggcgaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgat ggtgaaaaca ggtcgctggg ggctcgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002961
VERSION     KHUT01002961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaagggg actagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggatcgc
       61 taagtcaggg gtgaaatgcc aaggctcaac cttggaactg cctttgatac tgacgatctg
      121 gagattggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002962
VERSION     KHUT01002962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtggcc
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tgggttgctc
      121 gagcgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccatagctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002963
VERSION     KHUT01002963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgca ggcggttgat
       61 caagtgggat gtgaaagccc cgggcttaac ctgggaactg catcccggac tgattggctg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002964
VERSION     KHUT01002964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           62 c           83 g           43 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtcatg
       61 caagtcctcc gttaaatcca cctgctcaac agatgggctg cggaggatac tacaaggcta
      121 ggaggcggaa gaggcaagcg gtactcagtg ggtaggggta aaatcctctg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaat acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01002965             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002965
VERSION     KHUT01002965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           45 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 aaagtcgggt gtgaaacctc caggctcaac ctggagacgc cacctgatac tgctgtggct
      121 agagtccggt aggggagcac ggaattcccg gtgtagcggt gaaatgctta gatatcggga
      181 ggaacaccag tggcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01002966             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002966
VERSION     KHUT01002966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          102 g           38 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccat
       61 gcagtcggct cgtgaaagcc cggggctcaa ccccggaggt gcggccgaaa cccatgggct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggacctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002967
VERSION     KHUT01002967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttata
       61 taagattgtt gtgaaatccc tgggcttaac ctaggaactg caacgatgac tgtatagctt
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002968
VERSION     KHUT01002968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          100 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcatgg
       61 caagtaaggt gtgaaatctc cctgcctaac ggggagggtg cattttagac tgccaagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002969
VERSION     KHUT01002969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           78 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttaa
       61 taagtcagtg gtgaaagccg atcgcttaac gatcgaactg ccattgatac tgctaggctt
      121 gagtatagtt gaggtaggcg gaattgacag tgtagcggtg aaatgcatag atattgtcaa
      181 gaacaccaat tgcgaaggca gcttactaaa gtataactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01002970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002970
VERSION     KHUT01002970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           97 g           42 t
ORIGIN      
        1 tacagaggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttcga
       61 taagtcccgt gtgaaatccc tcggctcaac tgaggaatgg cacgggaaac tgacgggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002971
VERSION     KHUT01002971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggccgcacgg
       61 taagtcagat gtgaaatctc agggctcaac tctgaaattg catccgatac tgccgtgctt
      121 gaggactgga gaggagtctg gaattcatgg tggagcagtg aaatgcgtag atatcatgag
      181 gaacgctagt ggcgtaggcg agactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002972
VERSION     KHUT01002972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgtgta ggcgggtctt
       61 taagtgaggg gtgaaaggtt tgggcttaac ccagacactg cctttcatac tggagacctt
      121 gagtgcggga gaggcgagtg gaatggtcgg tgtagcggtg aaatgcgtag atatcgatcg
      181 gaacacccat ggcgaaggca gctcgctggc ctgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002973             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002973
VERSION     KHUT01002973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gcaagtccgg agtgaaatct caccgctcaa cggtgaggct gctttggata ctgcggatct
      121 ggagaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01002974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002974
VERSION     KHUT01002974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          102 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgggt
       61 caagtcgagt gtgaaagccc cgggctcaac ctgggaattg cgctcgagac tgggctgctt
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002975
VERSION     KHUT01002975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           84 g           50 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcgg
       61 aaagttgggt gtgaaatacc tcagcttaac tgaggaactg cgctcaaaac taccgtgctc
      121 gagggagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002976
VERSION     KHUT01002976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttatg
       61 taagttggat gtgaaagtcc tgggcttaac ctggggattg catccaaaac tacatgacta
      121 gagtagggta gagggaggta gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaataccagt ggcgaaggcg gcctcctgga ttcatactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01002977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002977
VERSION     KHUT01002977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          103 g           41 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggccttg
       61 caagtaccgt gtgaaatcca cctgctcaac gggtggggtg ctaggtagac tgcaaggcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002978
VERSION     KHUT01002978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c           99 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcgggctgg
       61 tcagtctggg gtgaaatctc acggctcaac cgtgagaggt ccctggatac tgctagcctt
      121 gaggggctca gaggagagtg gaattccggg tgtagtggtg gaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gctctctggg ggcaacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002979
VERSION     KHUT01002979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctatg
       61 caagtcgagt gtgaaatccc cgggctcaac ctgggaactg cattcgagac tgcattgctt
      121 gagtatggga gagggaggcg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg gcctcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002980
VERSION     KHUT01002980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcggttatt
       61 taagtctggt tttaaagacc ggggctcaac cccggggatg gactggagac tggatgacta
      121 gacctctgga gaggcaatcg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccaac ggcgaaggca gattgctgga cagaaggtga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01002981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002981
VERSION     KHUT01002981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcacgta ggcggccatt
       61 caagtcgatc gtgaaagctc ggggctcaac cccgagactg cggtcgatac tggatggcta
      121 gaggctggta ggggggtgtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gcaccctggg ccagtcctga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002982
VERSION     KHUT01002982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           83 g           53 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtaa
       61 caagtcactt gtgaaatctc cgagcttaac tcggaacggt caagtgatac tgttatgcta
      121 gagtacagaa agggtaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaggcg gattactagg ctgatactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002983
VERSION     KHUT01002983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggactta
       61 taagtcagtg gtgaaagctc agggctcaac cctggatgtg ccattgatac tgtaggtctt
      121 gaaagcagtc gaggtggctg gaatgtgtag tgtagcggtg aaatgcatag atattacaca
      181 gaacaccaat tgcgaaggca agtcactagg ctgtgtttga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002984
VERSION     KHUT01002984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcttgg
       61 taagttgaag gtgaaatctc ggggcttaac tccgaaactg ccttcaatac tgctgagctc
      121 gaggattgta gaggagaatg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg attctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002985
VERSION     KHUT01002985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggtctt
       61 taagtcagtg gtgaaatctc cgagctcaac ttggaaactg ccattgatac tattgatctt
      121 gaattctgtt gaggtgggcg gaataagtca tgtagcggtg aaatgcatag atatgactta
      181 gaacaccaat tgcgaaggca gctcactaaa cagatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01002986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002986
VERSION     KHUT01002986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc atggctcaac catggaactg ctgggcatac tggcgatctt
      121 gtgcaaccta ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtttgcaga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01002987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002987
VERSION     KHUT01002987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggttgga
       61 taagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgtccaactt
      121 gaaggcagga gagggaagca gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002988
VERSION     KHUT01002988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           50 c          106 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggcttgt
       61 cgcgtcggga gtgaaagccc gctgcttaac ggcgggttgg cttccggtac gggcaggctg
      121 gagtgtggca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01002989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002989
VERSION     KHUT01002989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtatg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccattgatac tatatatctt
      121 gaatcatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg gatgtattga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01002990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002990
VERSION     KHUT01002990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           81 g           58 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcctgg
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgccaggctt
      121 gaatttccag gaagcaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttgctact ggtggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01002991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU2999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002991
VERSION     KHUT01002991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU2999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           90 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcttt
       61 taagtttgac gtgaaagctc tcggcttaac tgggagaggt cgttgaatac tggaagactt
      121 gaggaaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc attttactga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01002992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002992
VERSION     KHUT01002992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           75 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggactgt
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tatcagtctt
      121 gaatgttctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgtaggca gctcactacg gaaatattga cgctgaggca cgaaagcgtg
      241 aggatcaaac agg
//
LOCUS       KHUT01002993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002993
VERSION     KHUT01002993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           91 g           48 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtaa ctgggcgtaa agggcgagta ggtggttgcg
       61 taagttggtt atgaaagctc ctggctaaac tgggagaggt tgaccaagac tgcgcgactt
      121 gagtggagaa gaggtaaacg gaattccgcg tgtagtggtg aaatgcgtag agatgcggaa
      181 gaacaccagt ggcgaaggcg gtttactggt ctccaactga cactgaaccg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01002994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002994
VERSION     KHUT01002994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctttg
       61 taagttagag gtgaaagccc ggggctcaac cccggaattg cctttaagac tgcatcgctt
      121 gaatcgcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgcgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01002995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002995
VERSION     KHUT01002995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           82 g           56 t
ORIGIN      
        1 gacgagtctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtggag
       61 taagtcggat gtgaaatacc ttagcttaac tagggaactg cactcgatac tactttgcta
      121 gagtaagaca gaggtgagtg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaggcg actcactggg tctttactga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01002996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002996
VERSION     KHUT01002996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           84 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggtggggcga
       61 taagtcagtg gtgaaatcct gcggcttaac cgtagaattg ccattgatac tgtcgctctt
      121 gagtacgctt gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agtgaagaca gctcacgaag gcgaaactga cactgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01002997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002997
VERSION     KHUT01002997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           92 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttgg
       61 taagtcttgt gtgaaagccc atggcctaac catggaagtg cacaggaaac tgctgaattt
      121 gagttcagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01002998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002998
VERSION     KHUT01002998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttttc
       61 taagtccgcg gttaaagccc ccggctcaac tgggggaggt ccgtggagac tagaggactg
      121 gagggcaaca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg acgtgctggg ttgcacctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01002999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01002999
VERSION     KHUT01002999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtctgg
       61 aaagttggat gtgaaagctc ccggcttaac tgggagaggt cgttcaaaac taccagactt
      121 gagtacagta gagggaagtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcttcctgga ctgaaactga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003000
VERSION     KHUT01003000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           88 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggatgtt
       61 taagtgggat gtgaaatact cgggctcaac ttgagtgctg cattccaaac tggatatcta
      121 gagtgcagga gaggaaagga gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaataccagt ggcgaaggcg cctttctgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003001
VERSION     KHUT01003001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatg
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaattg catttgagac tgcatggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003002
VERSION     KHUT01003002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           80 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggattta
       61 taagtcagtg gtgaaagccc gtcgcttaac gacggaactg ccattgatac tgtaagtctt
      121 gaattcgatc gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat agcgaaggca gctcactagg tctggattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003003
VERSION     KHUT01003003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtccgct gtgaaagtca agggctcaac ccttgaatgc cggtggatac tgccgaacta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003004
VERSION     KHUT01003004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcggat gtgaaagccc atggcttaac cgtgggtctg cattcgatac gggcaggcta
      121 gagttcggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccgatactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003005
VERSION     KHUT01003005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcgggcgat
       61 caagcgtgcg gtgaaagctc ggggctcaac tccgagtcgg ccgtgcgaac tggttgtcta
      121 gagtctggta gaggtcgatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtcgactggg ccagtactga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003006
VERSION     KHUT01003006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           90 g           44 t
ORIGIN      
        1 tacgaaccgg acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccccg
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaattg cgcccgatac tgcgtggcta
      121 gagggagaca gaggcgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcgctggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac agg
//
LOCUS       KHUT01003007             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003007
VERSION     KHUT01003007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgt
       61 caagtcgggt gtgaaacctc caggctcaac ctggagacgc cactcgagac tgtcatggct
      121 agagtccggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgatctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003008
VERSION     KHUT01003008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           82 g           53 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agagtgcgca ggcggaatat
       61 caagtcagga gtgaaatccc ggggctcaac ctcggaactg ctcttgaaac tgataatcta
      121 gagtcttgga gaggttagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actaactgga caagtactga cgctcatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003009
VERSION     KHUT01003009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           95 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccgcg
       61 caagtcggat gtgaaatccc acggctcaac cgtggaaggg cacccgaaac tgcgtggctc
      121 gagtcctgga gaggaaggtg gaatgctcgg tgtagaggtg aaattcgtag atatcgagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003010
VERSION     KHUT01003010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           81 g           58 t
ORIGIN      
        1 tacgtagggg gcaaacgttg tccggattta ctgggtgtaa agggtgctca ggcggtcttg
       61 taagttagaa gtgaaatcct agggctcaac tctggaactg cttttaatac tgcaaggctt
      121 gaatatggaa gaggagggtg gaatttctgg tgtagcagtg aaatgcgtag atatcagaaa
      181 gaacaccagt ggcgaaggcg gccctctggt ccattattga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003011
VERSION     KHUT01003011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggtcgag
       61 taagtcgaat gtgaaagccc cgggctcaac ccgggaagtg catccgaaac tgctcgacta
      121 gagtcccgga gaggaaggca gaattcccaa tgtaggggtg aaatccgtag atattgggag
      181 gaataccggt ggcgaaggcg gccttctgga cggtgactga cactcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003012
VERSION     KHUT01003012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacagagatg gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggagcga
       61 taggcgcctt gtgaaatccc tcggctcaac cgaggaactg cttggcgaac cggcgctctt
      121 gaggcaggta gaggtaactg gaaccatagg tggagcggtg aaatgcgtag agatctatgg
      181 gaacgccggt ggcgaaagcg agttactggg cctgttctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003013
VERSION     KHUT01003013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacggagtgt gcgagcgtta ctcggaatca ctgggcataa agagcacgta ggcggggatg
       61 catgtccgtg gtgaaatccc ccagctcaac tggggaactg ccgcggaaac tgtatctctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggc tcgtttctga cgctgaggtg cgaaagcttg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003014
VERSION     KHUT01003014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           88 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggctcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcccaaaac tactgagctt
      121 gagggagtta gaggtgagcg gaactcacgg tggagcggtg aaatgcgttg atatcgtgag
      181 gaacaccgga ggcgaaagcg gctcactggg gcttttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003015
VERSION     KHUT01003015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggtggctgtt
       61 caagtcgaat gtgaaaactc gaggctcaac ctcgagcctg cattcgatac tgggtggcta
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cactgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003016
VERSION     KHUT01003016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgg
       61 taagtcagct gtgaaatctc cgagcttaac tcggaacggt cagctgatac tgctgtgcta
      121 gagtacagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgatactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003017
VERSION     KHUT01003017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccgg
       61 taagtccgct gtgaaagtca agggctcaac ccttggatgc cggtggatac tgtcggacta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003018
VERSION     KHUT01003018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccgat
       61 taagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tggttggctt
      121 gagaccggaa gaggacagcg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctgtctggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003019
VERSION     KHUT01003019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           98 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagttcggt gtgaaagccc ccggcttacc cggggaactg cgccggaaac cgacagactc
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003020
VERSION     KHUT01003020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcttaa
       61 tcagtcaggg gtgaaatctt cccgcttaac gggaaacctg cctttgatac tgttgagctt
      121 gaattaggtt gaggtcggcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccaat tgcgaaggca gctgactagg cctgaattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003021
VERSION     KHUT01003021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           48 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgta ggcggccgag
       61 taagttggat gtgaaatctc ctggctcaac tgggagagga cgttcaagac tgttcggctg
      121 gaggacggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga ccgttcctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003022
VERSION     KHUT01003022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtttac
       61 caagtcgggg gtgaaatctt tcggctcaac cggaaaaggg ccttcgatac tggtaaacta
      121 gagtgaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003023
VERSION     KHUT01003023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           81 g           53 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggtcgtctt
      121 gagccactta ggggttaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtaactggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003024
VERSION     KHUT01003024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           62 c          102 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggccgtg
       61 tatgtgaccg gtgacagtcg cgggctcaac ccgcgccggc ccggttacac agcatagctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgtactga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003025
VERSION     KHUT01003025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacggaggga gcgaacgttg ttcggattca ctgggcgtaa agggcgtgta ggcggtctgg
       61 caagtcaggg gtgaaatacc acggctcaac cgtggaattg cctttgaaac taccggactt
      121 gaggccgtga gaggagactg gaattcccag tgtagcggtg aaatgcatag atattgggaa
      181 gaacaccagt ggcgaaggcg agtctctggc acggatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003026
VERSION     KHUT01003026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           98 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccgag
       61 gcagtcgggt gtgaaagccc ggagctcaac tccggaacgg cactcgaaac tccttggctg
      121 gaggccggaa gaggagagtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccagt ggcgaaagcg gctctctggt ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003027
VERSION     KHUT01003027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctaga
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tgtctagctt
      121 gaatctcgga gggggtcaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgacctgga cgaagattga cgctgaggtg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01003028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003028
VERSION     KHUT01003028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           78 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtacgca ggcggactgt
       61 taagtaggaa gtgaaaggtt ggagctcaac tccgacactg ctccctatac tggcagtctt
      121 gagtggcgga gaggaagatg gaacaagttg tgtagcggtg aaatgcgttg atataacttg
      181 gaacaccaat ggcgaaagca atcttctgga cgcaaactga cgctcatgta cgaaagctaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01003029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003029
VERSION     KHUT01003029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          106 g           41 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgccg
       61 caagtcgcgt gtgaaagccc cgggctcaac ctgggaggtg cgcgcgagac tgtggtgctg
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003030             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003030
VERSION     KHUT01003030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           55 c          101 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgca ggcggcctgg
       61 ccaagtcgtg cgtgaaatct ctcggcttaa ctgagagcgg tcgtgcgata ctggtcggct
      121 tgaggccggt agggggaagc ggaattccgg gtgtagtggt ggaatgcgta gatatccgga
      181 ggaacaccag tggcgaaggc ggcttcctgg accggtcctg acgctcagga gcgaaagcgt
      241 gggtagcgat ccgg
//
LOCUS       KHUT01003031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003031
VERSION     KHUT01003031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcatgca ggcggttcgc
       61 taagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaagac tgacgaactc
      121 gaggatggta gaggaaagcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gctttctgga ccatgactga cgctcagatg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003032
VERSION     KHUT01003032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tgccaagctt
      121 gagaatagta gaggcgggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctatttctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003033
VERSION     KHUT01003033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggtccgta ggcggtgtag
       61 caagtcgata gtgaaatctc tgggcttaac ccagaggctg ctaacgaaac tgctgtgcta
      121 gagtgtgaga gaggcgagtg gaattacggg tgtagcggtg aaatgcgtag atatccgtag
      181 gaacatccga ggcgaaggcg gctcgctgga tcacaactga cgctgaggga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003034
VERSION     KHUT01003034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 gcagtcaacg gtgaaagttc ggggctcaac cctgaatcgg ccgtcgatac tcccgtgctt
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003035
VERSION     KHUT01003035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcttaa agcgcgtgta ggcgggatgc
       61 caagtgtcgt gtgaaagccc cgggctcaac ccgggaattg cgcggcaaac tggtgttctt
      121 gaggcaagta gaggtgactg gaacgaccgg tggagcggtg aaatgcgtag atatcggttg
      181 gaacgccgga ggcgaaagcg ggtcactggg cttgtcctga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003036
VERSION     KHUT01003036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtaagat ttgaaatccc cgggcttaac ctgggaactg gatcttagac taatagactg
      121 gagtgcagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg accacctgga ctgagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003037
VERSION     KHUT01003037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           86 g           48 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgggaatctt
      121 gagctgctta ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggtgaaaaca gacgactggg agcatgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003038
VERSION     KHUT01003038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggccatt
       61 taagtcagag gtgaaagccc ggtgctcaac atcggaatag cctttgagac tgggtggcta
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003039
VERSION     KHUT01003039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           85 g           60 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccatt
       61 taagtcagcg gtgaaagtct gtggctcaac catggaactg ccgttgatac tgggtggctt
      121 gagtgttggt ggggtacatg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcgaaggca ttgtacttac caacaactga cgctgaggca cgaaagcgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01003040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003040
VERSION     KHUT01003040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          105 g           41 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtttca
       61 tacgtcgggt gtgaaagccc cccgctcaac gggggagggt cattcgaaac ggtggaactg
      121 gaggcaggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactgga cctgtactga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003041
VERSION     KHUT01003041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           86 g           53 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttgt
       61 taagttgggt gttaaagctt ccggctcaac cgggagttgt cgctcaaaac tagcagacta
      121 gaggatggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcctcctggc ccattcctga cactcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003042
VERSION     KHUT01003042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcacgca ggtggctgag
       61 aaagtcggat gtgaaagccc cgggcttaac ctgggaactg cattcgaaac tacttggcta
      121 gagtatggga gagggaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcttcctggc ccaatactga cactcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003043
VERSION     KHUT01003043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggaatgc
       61 caagtctggc ctgaaagccc ccggctcaac cggggatccg ggctggaaac tggttttctt
      121 gaggacggca ggggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gcactctgga ccggacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003044             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003044
VERSION     KHUT01003044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           40 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggtcgtg
       61 caagtgtcgt gtgaaagcag ctcgctcaac gagctgaggc atggcagact gcataactcg
      121 agcccgtgag aggtcggtgg aattgccggt gtagtggtga aatgcgtaga gatcggcagg
      181 aacacccaag gggaagccag ccgactggca cggtgctgac actgaggtgc gacagcgtgg
      241 ggagcaaacg gg
//
LOCUS       KHUT01003045             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003045
VERSION     KHUT01003045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggccgat
       61 taagtctggt gtttaacccc ggggctcaac cccgggtcgc actggaaact ggttggcttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003046
VERSION     KHUT01003046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggcgagt
       61 taagctagct gtgaaagccc cgggctcaac ctgggaactg cagttagaac tggctggcta
      121 gagtacggta gaggagtgtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacatcagt ggcgaaggcg acactctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003047
VERSION     KHUT01003047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           96 g           44 t
ORIGIN      
        1 tacggaggat gcaagcgttg ttcggaatca ctgggcttaa agagcatgta ggtggggtgt
       61 caggcgtcgg gtgaaatccc tcggctcaac cgaggaactg cctggcgaac cggcattctt
      121 gaggcaggta gaggtggctg gaacgacggg tggagcggtg aaatgcgtag atatccgttg
      181 gaacgccggc ggcgaaagcg agccactggg cctgtcctga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003048
VERSION     KHUT01003048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           79 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgca ggcgggctat
       61 taagtcagtg gtgaaaaccc ggggctcaac ctcggaactg ccattgatac tgatagtctt
      121 gagtttggtt gaagtgggcg gaatgtagca tgtagcggtg aaatgcatag atatgctaca
      181 gaacaccaat cgcgaaggca gctcactaag ccaaaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003049
VERSION     KHUT01003049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           77 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggtcgg
       61 tcagtcagcg gtgaaagtct gtggctcaac catagaattg ccattgatac tgccgtcctt
      121 gaatacctgt gaagtggttg gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaacaccgat tgcgaaggca gatcactaac aggtcattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003050
VERSION     KHUT01003050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatagt
       61 ttagtcagag gtaaaagccc agggctcaac cttggaactg cctttgatac tggctatctt
      121 gagtacggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003051
VERSION     KHUT01003051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcgagt
       61 tttgtcaggc gtgaaagccc cgggctcaac ctgggaattg cgcttgaaac gggcttgcta
      121 gagatcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003052
VERSION     KHUT01003052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           78 g           63 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattta
       61 taagtcagtg gtgaaagcct atagcttaac tatagaattg ccattgatac tgtaggtctt
      121 gagtacagtt gaggtgggcg gaatgtgcag tgtagcggtg aaatgcttag atattgcaca
      181 gaacaccaat tgcgaaggca gctcgctaag ctgtaactga cgctgaggca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003053
VERSION     KHUT01003053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tggatgtctt
      121 gagccatcta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003054
VERSION     KHUT01003054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          105 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcctgt
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgggaggctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003055
VERSION     KHUT01003055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           92 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggttttc
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac taggagactt
      121 gagtaccgga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga cggtcactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003056
VERSION     KHUT01003056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgc
       61 cacgtccgct gtgaaaacct agggcttaac cctgggcttg cagtagatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003057
VERSION     KHUT01003057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggagcgta ggcggccatg
       61 taagtgaggt gttaaatccc ccggctcaac cggggacctg cacctcatac tgcacggctt
      121 gagtacatca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg atgatactga cgctgatgct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003058
VERSION     KHUT01003058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggtggtctgt
       61 taagtcatgt gtgaaagccc ccggctcaac cggggaagtg cgcatgaaac tgacaagcta
      121 gagtaccaaa gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccctctggt tggatactga cgctaaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003059
VERSION     KHUT01003059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgc
       61 caagcgtgtg gtgaaagccc ggagctcaac tccgggtctg ccatgcgaac tggcaggcta
      121 gagcacggta gaggccaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttggctggg ccgttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003060
VERSION     KHUT01003060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtctgat gtgaaatccc ggggctcaac cctggaactg cattggatac tgccttgcta
      121 gagatctgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaaggccagt ggcgaaggcg agtctctgga cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003061
VERSION     KHUT01003061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcgagg
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tgcctagcta
      121 gagtcccgga gaggttggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gccaactgga cggtgactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003062
VERSION     KHUT01003062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           97 g           38 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggttgcg
       61 cgcgtggggt gtgaaaactg gcggcttaac tgccagaggc catcccatac ggcgcgactt
      121 gagccatgca gaggtccacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg catgcgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003063
VERSION     KHUT01003063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatcct gcagctcaac tgtagaagtg cctttgatac tggcgagctc
      121 gagggaggta ggggtgggtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acccactgga tctctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003064
VERSION     KHUT01003064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           93 g           46 t
ORIGIN      
        1 tacagagggc gcgagcgtta atcggattaa ctgggcgtaa agggcgcgta ggcggttatg
       61 tgagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gacatgactg
      121 gagtattgga gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gctacctggc caaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003065
VERSION     KHUT01003065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcggacatg
       61 taagtcagag gtgaaagcca ccggcctaac cggtgaactg cctttgatac tgcatgtctt
      121 gagtcctgga ggggtcacta gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaataccaga ggcgaaggcg ggtgactgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003066
VERSION     KHUT01003066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          100 g           43 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcactt
       61 caagtacgga gtgaaatctc cttgctcaac gaggagggtg ctttgtaaac tggagagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003067
VERSION     KHUT01003067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           37 c           73 g           67 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcaat
       61 taagtcagtg gtgaaagttt gcagctcaac tgtaaaattg ccattgatac tggttgtctt
      121 gaattactat gaagtggtta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca gatcactaat agtacattga cgctgaggga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003068
VERSION     KHUT01003068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          105 g           39 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgctgggctg
      121 gagcatcgta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag agatccgaaa
      181 gaacaccagt ggcgaaggcg gcctgctggg cgattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003069
VERSION     KHUT01003069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcccgt
       61 taagtcgggt gtgaaaatcc ggggctcaac cccggacctg catccgatac tggcgggcta
      121 gaggcagaca ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg tctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003070
VERSION     KHUT01003070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           83 g           55 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtctgg
       61 caagtctgat attaaagact ggagcttaac ttcaggactg tatcggaaac tgccaaacta
      121 gaggactata gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg gacttctggg tagttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003071
VERSION     KHUT01003071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           42 c           93 g           58 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtacgta ggcggtttgc
       61 taagtctggt gtgaaatctc ctggcttaac tgggagggtg cgctagatac tggcaggctt
      121 gagtatggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggtgtagacg gctctctgga ccattactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003072
VERSION     KHUT01003072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           93 g           41 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatca ctgggcataa agggcgcgta ggcggcgagg
       61 caagtcgggt gtgaaatccc tcagctcaac tgaggaactg cgcccgaaac taccttgcta
      121 gagtgcagga gaggagagtg gaactcctag tggagcggtg aaatgcgtag agattaggag
      181 gaacgccggt ggcgaaagcg actctctggc ctgctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003073
VERSION     KHUT01003073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 gacggagggt gcgaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggacctg
       61 taagtcggga gtgaaaactc ggggctcaac cccgagcctg cttccgaaac tgcaggtctt
      121 gagggccgga gaggagagtg gaattcctag tgtagcggtg gaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg actctctgga cggctcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003074
VERSION     KHUT01003074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 taagtggaat gtgaaatccc tcggctcacc cgaggacctg catcccaaac tgccgagctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003075
VERSION     KHUT01003075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           91 g           53 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggagcgta ggcggttttg
       61 taagtcgtgt gtgaaatctt cgggctcaac ccgaagtctg cacacgaaac tgcagggctt
      121 gagtatggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccattactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003076
VERSION     KHUT01003076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgca ggcggttcga
       61 ttagtcagaa gtgaaagccc cgggctcaac ctgggaactg cttttgatac tgtcgagctt
      121 gaatcacgga gagggtagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actacctggc cgtcgattga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003077
VERSION     KHUT01003077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           99 g           48 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggtggttgga
       61 tacgtctgct gtgaaatgtc ccggcttaac cgggagggtg cggcagatac ggtctgactt
      121 gagggtgaca gaggagcgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgcaagcg gcgctctggg tcacatctga cactgagacg cgaaggcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003078
VERSION     KHUT01003078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggcatgg
       61 tcagtctgct gtgaaagctc tcggcttaac cgggagaggt cggtagaaac tgccaagctt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003079
VERSION     KHUT01003079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c          105 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcgggaggc
       61 caagtctgtg gtgaaatcgc ctggcttaac tgggcggggt ccatggaaac tggtcacctg
      121 gaggtcggta gaggcaagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcttgctggg ccggacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003080
VERSION     KHUT01003080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgagg
       61 aaagtcagat gtgaaatccg agggctcaac cctcgaatag catccgatac ttccctgctc
      121 gagatttgta gaggagtctg gaactctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacgctcgt agcgaaggcg aggctctggg caagttctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003081
VERSION     KHUT01003081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcttcg
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgcggagcta
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003082
VERSION     KHUT01003082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcttgatac tgccgtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003083
VERSION     KHUT01003083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcggac gtgaaatccc tcggctcaac cgaggaactg cgtccgatac tggttggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgatattga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003084
VERSION     KHUT01003084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           82 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggactgt
       61 taagtaggaa gtgaaaggtc ggagctcaac tccaacattg cttcctatac tggcagtctt
      121 gagtagcgga gaggaagggg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacacccat ggcgaaagca cccttctgga cgcaaactga cgctcaggtt cgaaagctaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01003085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003085
VERSION     KHUT01003085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcggggtct
       61 taagtaaagt gtttaatccc ggggcccaac cccggaccgg cattttaaac tgggactctt
      121 gaatagtgga gaggtcggtg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacaccagt tgcgaaggcg gccgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003086
VERSION     KHUT01003086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           60 c          108 g           38 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcgcat
       61 caagtctggc gtgaaagccc ccggctcaac cggggagggt cgtcggagac tgacgcgctt
      121 gagggcggta ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccagctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003087
VERSION     KHUT01003087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcgtt
       61 taagtcagtg gtgaaatctc cgagctcaac ttggaaactg ccattgatac tattcgtctt
      121 gaatatcgtt gaggtaggcg gaataagtca tgtagcggtg aaatgcatag atatgactta
      181 gaacaccaat tgcgaaggca gcttactaaa cgatcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003088
VERSION     KHUT01003088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggagcgta ggcggccaag
       61 taagtggggt gttaaatctc ccggctcaac cgggaacctg cacctcatac tgcatggctt
      121 gagtacagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg ctgtatctga cgctgatgct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003089
VERSION     KHUT01003089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcgcgc
       61 taagtcggga gtgaaagccc tcggctcaac cgaggaatgt ctttcgatac tggcgtgctt
      121 gaggacggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactggg ccgtctctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003090
VERSION     KHUT01003090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggttggg
       61 taagttggct gtgaaatctc ccggctcaac tgggagggga cggtcaagac tgctcgactg
      121 gaggcaggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg acctcctgga cctgtcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003091
VERSION     KHUT01003091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcgag
       61 taagtggaat gtgaaatact ccggctcacc cggagcactg cattccaaac tgctcggctt
      121 gagtatggga gaggacaagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cttgtctggc ccaatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003092
VERSION     KHUT01003092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           82 g           58 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agagggcgta ggtggctatt
       61 taagtcagct gtgaaagcct ggggctcaac cccagaagtg cagttgatac tatttagctt
      121 gagtgtggaa aaggtaatta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaatgccggt tgcgaaggcg gattactagt ccaacactga cactgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003093
VERSION     KHUT01003093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           85 g           53 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgcc
       61 taagtacctt gtgaaatccc acagcttaac tgtggaactg cttggtatac tgggtgtctt
      121 gagtcactca ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtgcactga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003094
VERSION     KHUT01003094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c          103 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggtctga
       61 cgcgttttgt gtgacagcgg gtggcttaac tatccgagca tatgaaagac ggttggactt
      121 gagggctcga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc gagtgactga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003095
VERSION     KHUT01003095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           76 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcttg
       61 taagtcagtg gtgaaatccg gcagcttaac tgtcgaactg ccattgatac tgtgagtctt
      121 gaattactat gaagtggtta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca gatcactaat agtgtattga cgctgaggga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003096
VERSION     KHUT01003096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggtcagg
       61 taagtccggt gtgaaagtca taggctcaac ctatgaatgc cgctggatac tgcctggctt
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctgga acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003097
VERSION     KHUT01003097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggattta ctgggcgtaa agcatgcgca ggcggcggtt
       61 caagtcaggt gtgaaatccc cgagcttaac ttgggaattg cgcttgaaac tagatcgcta
      121 gagtacggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg cctgtactga cgctcatgca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003098
VERSION     KHUT01003098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          101 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtgcgg
       61 taagtcgggc gtgaaatccc cgggcttaac ccggggcggt cgttcgaaac tgccgtgcta
      121 gagtgcagga gagggaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggcgaaggcg gcttcctgga ctgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003099
VERSION     KHUT01003099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gatggtttgt
       61 taagtcgaat gtgaaagccc cgggctcaac ctgggaactg catccgatac tggcagacta
      121 gagtgtggta gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga ccaacactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003100
VERSION     KHUT01003100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgct
       61 taagtcctgc gtgaaatctc ggagctcaac tccgagcggt cgtgggaaac tgggcggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003101
VERSION     KHUT01003101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           98 g           43 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttcga
       61 taagtccgct gttaaagccc ccggctcaac tgggggaggt cagtggagac tataggactt
      121 gagggcaaca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg acgtgctggg ttgcgactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003102
VERSION     KHUT01003102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtcaggg gtgaaagccc acggcttaac cgtggaagtg cctttgatac tgctggtctt
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003103
VERSION     KHUT01003103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           97 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggagtta ctgggcgtaa agggcgcgta ggcggcgctc
       61 caagttaggc gtgaaagccc cgggctcaac ctgggaattg cgcttaagac tggagtgctc
      121 gagtgcggaa gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003104
VERSION     KHUT01003104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacgtaggct ccaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtccgt
       61 caagttggtt gtgaaatctc ctggctcaac tgggagggtg cgaccaaaac tgacggacta
      121 gagtctggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgaagacg gctctctgga ccggtactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003105
VERSION     KHUT01003105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacagaggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccatt
       61 taagtcagac gtgaaatccc caggctcaac ctgggaactg cgtctgatac tgggtggctt
      121 ggatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg acatcctgga ccggcatcga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003106
VERSION     KHUT01003106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           54 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaatt
       61 caagtcactt gtgaaatctc cgagcttaac tcggaacggt caagtgatac tgtcttgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003107
VERSION     KHUT01003107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           98 g           49 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtcttg
       61 tgtgtctatc gtgaaagcac acggctcaac cgtgtaaagt cggtggaaac tgcaggactt
      121 gaggttggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacacttgt ggcgaaggcg gccagctgga ccaatcctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003108
VERSION     KHUT01003108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttgt
       61 taagtcgggt gtgaaagccc tcagctcaac tgaggaaggg cgcccgaaac tggcgagctt
      121 gagtgtcgga gaggatggcg gaatacccca agtagaggtg aaattcgtag atatggggtg
      181 gaacaccggt ggcgaaggcg gccatctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003109
VERSION     KHUT01003109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           92 g           50 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggccttt
       61 taagtccttt gtgaaattcc ggagctcaac tccggatgtg cataggatac tggaaggcta
      121 gagactggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccagttctga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003110
VERSION     KHUT01003110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ctgggcgtaa agcgcacgta ggcggaccag
       61 aaagtagggg gtgaaatccc ggggctcaac ctcggaactg ccttctaaac tcctggtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003111
VERSION     KHUT01003111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgagta ggtggcttgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tgacaagctt
      121 gagcaaagta gaggcgagtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg actcgctggg cttttgctga cactgaatca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003112             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003112
VERSION     KHUT01003112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           98 g           40 t
ORIGIN      
        1 aacgtaggac gcaaacgtta tccggaatta ctgggcgtaa agggcgcgca ggcggaacgg
       61 taagtgaggc atgaaagctc cctggctaaa ccgggagagg atgtctgaga ctgccggact
      121 ggagtgaggt agaggggtgt ggaattccgc gtgtagtggt gaaatgcgta gagatgcgga
      181 ggaacaccag tggcgaaggc ggcaccctgg accttaactg acgctgaggc gcgaaagcat
      241 gggtagcgaa cggg
//
LOCUS       KHUT01003113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003113
VERSION     KHUT01003113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgagggga gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgta
       61 ccagtctggg gtgaaagccc tcggctcaac cgaggaattg ccttggaaac tgtacggctt
      121 gagtattgga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgt ggcgaaggcg gctttctgga caatcactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003114
VERSION     KHUT01003114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           88 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggccaa
       61 caagtcagat gtgaaatccc tgggctcaac ccaggaactg catttgaaac tgttggcctt
      121 gagtggtgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003115
VERSION     KHUT01003115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacac
       61 taagtacaca gtgaaatctc cttgcctaac gaggagggtg ctgagtaaac tggtgagcta
      121 gagagcggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtatctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003116
VERSION     KHUT01003116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggagcgta ggcggtctgt
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgacggactt
      121 gaatatagtt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactaag ctatgattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003117
VERSION     KHUT01003117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           77 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcaggg gtgaaatacg gcagctcaac tgtcgcagtg cctttgatac tgatgagctt
      121 gaatattgat gaagatggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gctatctaag caatgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003118
VERSION     KHUT01003118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctaga
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg cattcgatac tgtttagcta
      121 gagtatgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggc ttaatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003119
VERSION     KHUT01003119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtctgac gtgaaatccc cgggcttaac ctgggaatgg cgttggagac tgcaaggctg
      121 gaatctggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaagattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003120
VERSION     KHUT01003120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcgggactg
       61 taagtcagag gtgaaagccc acggctcaac cgtggaattg cctttgatac tgcagttctt
      121 gaatcctgga gaggtcacta gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaataccaga ggcgtaggcg ggtgactgga caggtattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003121
VERSION     KHUT01003121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           85 g           47 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggcggag
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaacag cgccctaaac tactttgctt
      121 gaggaggcta gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg acctttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003122
VERSION     KHUT01003122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 caagtcagag gtgaaagccc agggctcaac cctggaattg cctttgagac tgcatcgcta
      121 gaacacggga gaggtaagcg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactgga ccgttgttga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003123
VERSION     KHUT01003123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           96 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggattta ttgggcgtaa agggcgcgta ggcggtttgg
       61 taagtcaggt gtgaaatccc ggggcttaac cccggaagtg catttgagac tgccagactt
      121 gagtatggga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccaatactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003124
VERSION     KHUT01003124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           96 g           41 t
ORIGIN      
        1 aacgtagggg tcgagcgtta tccggaatta ctgggcgtaa agcgcgtgca ggcggcgacg
       61 caagttggac gtgaaagctc ctggctcaac tgggagaggc cgttcaaaac tgcgtcactt
      121 gaggatggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctggg ccataactga cgctgagacg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01003125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003125
VERSION     KHUT01003125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           61 c          112 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggcctcg
       61 cgcgcccgac gtgaaagccc ccggcttaac cggggagagt cgttggggac ggcggggctt
      121 gagggcggga gagggtggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccacctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003126
VERSION     KHUT01003126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           94 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccaga
       61 caagtcgggt gtgaaagccc acggctcaac cgtggaaggg cactcgaaac tgcttggcta
      121 gagttctgga gaggaaggcg gaatactcgg tgtagaggtg aaattcgtag atatcgagtg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003127
VERSION     KHUT01003127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtccgct gtgaaagtca agggctcaac ccttgaatgc cggcggatac tgccaggcta
      121 gagtccggaa gaggtgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcactggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003128
VERSION     KHUT01003128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggacttt
       61 taagtccgtg gtgaaatctc cgggctcaac ccggaaactg ccatggatac tattggtctt
      121 gaatgttgtg gaggtgggcg gaacgggtca tgtagcggtg aaatgcttag agatgacccg
      181 gaacaccgat tgcgaaggca gcccactacg caactattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003129
VERSION     KHUT01003129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttat
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaatgg catttgagac tggtaggcta
      121 gagtatggta gaggtgagtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg actcactggg ccattactga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003130
VERSION     KHUT01003130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggctctt
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaaaac tggggagctt
      121 gaggcaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctagc gttgtactga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003131
VERSION     KHUT01003131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgcgtgta ggcggtccgg
       61 taagttggct gtgaaatctc ccggctcaac tgggagagga cggtcaagac tgtcggactg
      121 gaggacggta gagggaggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcctcctggg ccgttcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003132
VERSION     KHUT01003132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c          100 g           40 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagttcggc gtgaaagcgc ctggctaaac cgggcgaggt cgtcggagac tggcagacta
      121 gagggtgata gaggcgggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acccgctggg tcacacctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003133
VERSION     KHUT01003133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           99 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgggt gtgaaagccc acagcttaac tgtgggtctg cattcgatac gggcagacta
      121 gaggcaggta ggggagcatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gtgctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003134
VERSION     KHUT01003134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          103 g           42 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattca ctgggcgtac agggagcgta ggcggttggg
       61 taagccctcc gtgaaatctc cgggcctaac ccggaaagtg cagaggggac tgctcggctt
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtttctga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003135
VERSION     KHUT01003135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           93 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcttc
       61 taagtctggt ttgaaagcag gcggcttaac cgtctgatgt ggctggaaac tgggggactt
      121 gaatgggttg gcggtcgccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggcgactagg acccgattga cactgaggca cgagagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003136
VERSION     KHUT01003136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgac
       61 taagtcagat gtgaaatccc ccggcttaac cggggaactg catctgatac tggtgggctt
      121 gaatccggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003137
VERSION     KHUT01003137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           48 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggtggcttgg
       61 caagtggaag gtgaaatccc tcggctcaac tgaggaactg ccttccagac tgctttgctt
      121 gaggccggga gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actcgctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003138
VERSION     KHUT01003138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           56 c          108 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggtcgat
       61 tgcgtcgtgc gtgaaggccc tcggctcaac cgggggtggt cgtgcgatac gggtcgactg
      121 gaggtaggta gggggacgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggc ggcgaaggcg gcgtcctgga cctggcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01003139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003139
VERSION     KHUT01003139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggctgcg
       61 taagtctgac gtgaaatctc ccggcttaac cgggagcggt cgttggaaac tatgcggctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaagcg gcaccctggc attagcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003140
VERSION     KHUT01003140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           99 g           37 t
ORIGIN      
        1 aacgtaggac gcgagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtctga
       61 caagtctgat gtgaaatctc ccggcttaac cgggcgaggc cattggaaac tgacaggctg
      121 gagggcagta gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003141
VERSION     KHUT01003141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccga
       61 taagtcctgt gtgaaatccc tcggctcaac tgaggaactg cacgggagac tgtcgggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003142
VERSION     KHUT01003142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          101 g           38 t
ORIGIN      
        1 gacgtagggg gcgagcgtta cccggattta ctgggcgtaa agcgcgcgca ggcggccgcg
       61 taagtcggat gtgaaagccc acggctcaac cgaggaattg catccgatac tgtgcggcta
      121 gaggcaggta ggggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctcgctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003143
VERSION     KHUT01003143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttcg
       61 caagtcggat gtgaaagccc acggctcaac cgtggaagtg cacccgaaac tgcgtggctt
      121 gagttctgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003144
VERSION     KHUT01003144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           91 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaccgg
       61 aaagtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac ttccggtctt
      121 gagtggtgga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003145             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003145
VERSION     KHUT01003145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctgt
       61 taagaccgat gtgaaatccc cgggcttaac ctgggaactg cattggtgac tggcaggctt
      121 tgagtgtggc agagggaggt agaattccac gtgtagcagt gaaatgcgta gagatgtgga
      181 ggaataccga tggcgaaggc agcctcctgg gccaacactg acgctcatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003146
VERSION     KHUT01003146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c          101 g           36 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcgggtgcg
       61 cgcgtggggt gtgaaacctg gccgcttaac ggccagcggc catcccatac ggcgcgcctg
      121 gagccttgca gagggcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggagaaatca gccgcctggg caagtgctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003147
VERSION     KHUT01003147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 ttagtcagac gtgaaatccc taggcttaac ctaggaactg cgtctgatac tggaaggcta
      121 gagttcggga gaggaatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccgaaactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003148             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003148
VERSION     KHUT01003148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           87 g           50 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agggtctgta ggtggttttg
       61 acagtctgct gttaaatctc agggctcaac cctgttaagg cagcatgaaa ctacgaatct
      121 tgagtacggt aggggcagag ggaattcccg gtggagtggt gaaatacgta gatctcggga
      181 agaacaccaa aggcgaaagc actctgctgg gccgagactg acactgaggg acgagagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01003149             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003149
VERSION     KHUT01003149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c          100 g           50 t
ORIGIN      
        1 tacggaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggggaat
       61 taagtgagaa gtgaaaggct ggggctcaac ctcatgcagt gcttctcata ctggttctct
      121 tgagggcggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccgtttctg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003150             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003150
VERSION     KHUT01003150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c          101 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctctt
       61 taagtccgct ggtcaaagac cggggctcaa ccccgcgtca gccagggata ctggagggct
      121 ggagacaggt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg acctggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003151             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003151
VERSION     KHUT01003151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           93 g           43 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtgtccg cgtgaaatcc cacggctcaa ccgtggaact gccgggcata ctggcgacct
      121 tgaggcactt aggggcgagc ggaacaaatg gtggagcggt gaaatgcgta gatatcattt
      181 ggaacgccga cggtgaaaac agctcgctgg gagtgtcctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003152
VERSION     KHUT01003152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           98 g           42 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggccgcg
       61 caagtcggat gtgaaatccc gaggctcaac ctcgggcgtg catccgatac tgcgtggcta
      121 gaggtaggta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003153             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003153
VERSION     KHUT01003153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           48 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcatga
       61 caagtcactt gtgaaatctc cgggcttaac tcggaacggc caagtgatac tgtcacgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgagtgc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003154
VERSION     KHUT01003154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           90 g           49 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttgcc
       61 atagtcaggt gtgaaagcct tgagcttaac tcaagaaatg cacttgatac tgggcgacta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggttactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003155             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003155
VERSION     KHUT01003155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggact gcaggggata ctgcgaggct
      121 agagacaagc agaggttggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggccaactgg gcttgttctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003156             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003156
VERSION     KHUT01003156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           87 g           48 t
ORIGIN      
        1 tacagaggga gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtccgt
       61 aaagtttaat gtcaaatacc agggcttaac cctggaccgg cattgaagta ctcacggact
      121 tgagtacggt agaggcagag ggaattccat gtggagcggt gaaatgcgta gagatatgga
      181 ggaacaccag tggcgaaggc gctctgctgg gccgaaactg acactgagag acgaaagctg
      241 ggggagcaaa tagg
//
LOCUS       KHUT01003157             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003157
VERSION     KHUT01003157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttgg
       61 taggtcttgc gtcaaaacct gaggctcaac ctcagttcgc gtgggaaacc atctagctag
      121 agtccggaag aggagaacgg aattcccagt gtagcggtga aatgcgcaga tattgggaag
      181 aacaccagtg gcgaaggcgg ttctctggga cggtactgac gctgagacgc gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01003158             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003158
VERSION     KHUT01003158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           88 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcatt
       61 taagtctggt gtttaatcct ggggctcaac cccagttcgc atcggaaact ggatgacttg
      121 agtgtaagag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggct tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003159             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003159
VERSION     KHUT01003159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           95 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggtgggatca
       61 taagtctttt gtcaaagtgc ggggctcaac cccgtgatgc ggaggaaact gtgattcttg
      121 agtgccggag aggaaagcgg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtg gcgaaggcgg ctttctggac ggtgtctgac actgaggcgc gaaagccagg
      241 ggagcgaacg gg
//
LOCUS       KHUT01003160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003160
VERSION     KHUT01003160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggcaagg
       61 taagttcgat gtgaaatccc cgggcttaac tcgggaactg cattgaatac tctcttgcta
      121 gaggatcgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003161
VERSION     KHUT01003161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggccgcg
       61 caagtcgggt gtgaaatccc ggagctcaac tccggaactg cactcgaaac tgcgcggctt
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003162
VERSION     KHUT01003162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggtttaa agggcgcgta ggcgggctgt
       61 caagtcggtg gtgaaatctt tcggctcaac cggaaaattg ccttcgatac tggcggcctg
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003163
VERSION     KHUT01003163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           98 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctct
       61 taagtctggt gtgaaatccc ggggcttacc cccggaagtg cattggatac tgggaggctc
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003164
VERSION     KHUT01003164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           77 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtccgca ggcgggagaa
       61 taagtcagag gtgaaatcct gcagcttaac tgtagaattg cctttgatac tgtttttctt
      121 gagtgtgatt gaagtggatg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gttcactaag tcataactga cgctgaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003165
VERSION     KHUT01003165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttctt
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tgaagggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtaaacg gctctctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003166
VERSION     KHUT01003166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           94 g           43 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggcggatggt
       61 taagtcattg gtgaaagctc cgggctaaac tcggagaggt ccgatgatac tgatcatcta
      121 gagggggtca gaggggtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg acaccctggg accaacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003167
VERSION     KHUT01003167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           97 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttcgg
       61 tcagtcggat gtgaaagccc cgggcttaac ttgggaattg cattcgatac tgccgggcta
      121 gagtgtggta gagggaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcttcctgga ccaacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003168
VERSION     KHUT01003168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           75 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtggacggt
       61 taagtcaggg gtgaaagtct gcagctcaac tgtagaactg cccttgatac tggtcgtctt
      121 gaatcattgt gaagtggtta gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaataccgat tgcgaaggca gatcactaac aatgcattga cactgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003169
VERSION     KHUT01003169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcctcg
       61 caagctggat gtgaaagccc cgggctcaac ccgggaagtg cacccagaac tgcttggctt
      121 gagttccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003170
VERSION     KHUT01003170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgca ggcgggtttg
       61 caagtcagtg gtgaaagcct acagcttaac tgtagaactg ccattgatac tgcagatctt
      121 gagtacggaa gagagagacg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt tgcgaaggcg gtctcttggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003171
VERSION     KHUT01003171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacggagacc tcgagcgtta ctcggattta ctgggcgtaa agggttcgta ggcggcctga
       61 tgtgttgggt gtgaaatcct gcagcttaac tgcagaactg cgctcaaaac tatcaggcta
      121 gagtgttgga gaggtgagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacaccaac ggcgaaggca gctcactgga caactactga cgctgaggaa cgaaagtgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01003172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003172
VERSION     KHUT01003172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctaag
       61 taagccggtt gtgaaagccc cgggctcaac ctgggaacgg catccggaac tgtttagcta
      121 gagtgcagga gaggaaggta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003173
VERSION     KHUT01003173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctat
       61 taagttggat gtgaaatctc cgggctcaac ccggaaaccg catccaaaac tggcagcctt
      121 gaggtatgga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcttcctggt catatcctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003174
VERSION     KHUT01003174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgacc
       61 caagtccatg gtgaaagctc gatgctcaac atcgagtcgg ccacggatac tgggttgctg
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003175
VERSION     KHUT01003175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggttga
       61 taagtcagtg gtgaaagccc cgggcctaac ccgggaactg ccattgatac tatcagtctt
      121 gaatacagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003176             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003176
VERSION     KHUT01003176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          103 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agagcgcgta ggcggcgcga
       61 tacgacaggt gtgaaagccc ccggctcaac tggggagggt catctgtaac ggtcgtgctc
      121 tgagtgcagg agagggacgt ggaactccgg gagtagcggt gaaatgcgta gagacccgga
      181 ggaacaccag tggcgaaggc ggcgtcctgg cctgcgactg acgctgtggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003177
VERSION     KHUT01003177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccact
       61 taagtcagac gtgaaatccc ttggctcaac caaggaactg cgtctgatac tgagtggctt
      121 gagtccggga gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccggtactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003178
VERSION     KHUT01003178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctcgt
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaagtg catccgaaac tagcgagctt
      121 gagtatggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003179
VERSION     KHUT01003179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           86 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggttggg
       61 taagtccgac gtgaaatctc cgagcctaac tcggaaactg catcggatac tattcaactc
      121 gagggatgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003180
VERSION     KHUT01003180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtctgtt gtgaaatccc ggagcttaac tccggaacgg catcggatac tatctgactt
      121 gagggttgaa ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caatacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003181             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003181
VERSION     KHUT01003181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           47 c           99 g           55 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggtttgt
       61 cgcgtctgcc gtgaaagcct gtggcttaac tgtgggtttg cggtggatac gggcataact
      121 tgaggcaggt aggggagaat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccgg tggcgaaggc ggttctctgg gccttgcctg acactgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003182
VERSION     KHUT01003182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           79 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcgggaaga
       61 taagtcagtg gtgaaatctc acagctcaac tgtgaaactg ccattgatac tgtctttctt
      121 gaattcgatc gaagtgggcg gaatgtgaca tgtagcggtg aaatgcttag atatgtcaca
      181 gaacaccgat agcgaaggca gctcactagg tctggattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003183
VERSION     KHUT01003183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcgggg gtgaaatccg gcagcttaac tgtcgaagtg ccttcgatac tggcgagcta
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003184
VERSION     KHUT01003184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          101 g           43 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggctggc
       61 gaagtcgtgt gttaaagccc ctggctcaac tgggggaggt catgcgagac tcgtcggctt
      121 gagggtcaca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg acgtgctggg tggcaactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003185
VERSION     KHUT01003185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           80 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtttga
       61 taagtcagag gtgaaagctc atcgcttaac gatgaaacgg cctttgatac tgtcagactt
      121 gaattaggat aaggtaagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccaat tgcgaaggca gcttactggg ccttgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003186
VERSION     KHUT01003186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctatc
       61 cacgtcggat gtgaaagctc atggctcaac cgtgagaggc cattcgatac gggatagcta
      121 gaggcagcta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg gctgttctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003187
VERSION     KHUT01003187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 cgcgtcgtgc gtgaaaatcc accgctcaac ggtggacttg cgtacgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003188
VERSION     KHUT01003188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          105 g           35 t
ORIGIN      
        1 cacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtcggg
       61 ttcgtcgctg gtgaaagccc cacgctcaac gtgggaaggt ccggcgagac agcccgactg
      121 gaggcaggga gaggtcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gccgactgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003189             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003189
VERSION     KHUT01003189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           99 a           26 c           61 g           68 t
ORIGIN      
        1 tacgggagga gcgagcatta ttcaaaatga ttaggcgtaa agggtttgta ggtggttttt
       61 taagttgaaa aaaaaagaat aaagctcaac tttatacatt ttttcaaaac tgaaaaacta
      121 gagtacatat agaggataat ggaattttta ttgtagggat aaaatatatt gataatagaa
      181 ggaacatcaa aagcgaaggc aattatctgg gtctgtactg acactgagaa acgaaagctt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003190
VERSION     KHUT01003190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          106 g           37 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttcg
       61 tgcgtcggtg ctgaaagcgc cgggcttaac ccggcgaggc gtgccgatac gacgaggctt
      121 gaggcaggga gagggtagcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgccaga gacgaaggtg gctacctggc cctgacctga cactgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003191
VERSION     KHUT01003191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggtggtttgc
       61 caagtcgggg ttgaaatctt tcggcttaac cggaaaagtg gcttcgatac tggcaagctg
      121 gagggaggta ggggcggatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga cctcttctga cactgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003192
VERSION     KHUT01003192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggtggctgag
       61 atagtcgatt gtgaaagccc tgggcttaac ctgggaattg cattcgatac tactcggcta
      121 gagtatgaga gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctgcctggc tcaatactga cactcaggtg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003193
VERSION     KHUT01003193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           88 g           48 t
ORIGIN      
        1 gacgaacctg acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcca
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaatac tgtggtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt agcgaaagcg gctcactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003194
VERSION     KHUT01003194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taggtcggtt gtgaaaactg gaggctcaac ctctagacgt cgaccgaaac catttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003195
VERSION     KHUT01003195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           93 g           40 t
ORIGIN      
        1 gacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggagcg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tgcgcttctt
      121 gggccaacta ggggcagccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggctgctggg gttgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003196
VERSION     KHUT01003196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcaag
       61 taagtggggt gtgaaatccc tcggctcacc cgaggacctg cattccaaac tgcttggctt
      121 gagtacggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003197             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003197
VERSION     KHUT01003197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           79 g           63 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttaa
       61 taagtcggtg gtcaaaggta gcagcttaac tgttttacat gccaccgata ctgttgagct
      121 tgagatatgt ggaggcagat ggaatttccg gtgtagcggt gaaatgcata gataccggaa
      181 ggaactccta ttgcgaaggc aatctgctac agataaactg acgctgatgc acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01003198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003198
VERSION     KHUT01003198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           84 g           57 t
ORIGIN      
        1 tacgtatggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgca ggtggtttgt
       61 taagttggtg gtttaatctc tgggctcaac ccagagtcag ccatcaaaac tggcgaactt
      121 gagtacgata ggggatagcg gaattctcgg tgtagcggtg gaatgcgtag atatcgagag
      181 gaacaccaat ggcgaaggca gctatctgga tcgtaactga cactcatgag cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01003199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003199
VERSION     KHUT01003199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcactt gtgaaatctc tgggctcaac tcagagtctg caagcgaaac tgccgtgctt
      121 gagtgtcgga gaggtaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcttactaga cgacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003200
VERSION     KHUT01003200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggctatc
       61 cgcgtcggat gtgaaagctc ggagctcaac tctgagaggc cattcgatac gggatagcta
      121 gaggtaggta ggggaaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003201
VERSION     KHUT01003201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           83 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggctgcgcgg
       61 caagtcaaat gtgaaattca ggggctcaac ccctgcattg cgcttgatac ttccgcgctc
      121 gagccttgga aaggagtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacactaag ggcgaaggca agactctgga caagtgctga cgctcatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003202
VERSION     KHUT01003202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           95 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggctcag
       61 caagtttcag gtgaaatctc ctggctcaac tgggaggggt cttggaaaac tgctgggctt
      121 gaggcaggga gagggatgcg gaataccagg tgtagtggtg aaatgcgtag atatctggtg
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01003203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003203
VERSION     KHUT01003203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctttt
       61 taagccagat gtgaaatccc cgggcttaac ctgggaactg catttggaac tgggaggcta
      121 gagtgtagca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccaat ggcgaaggca gccccctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003204
VERSION     KHUT01003204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           99 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctgct gtgaaaacct agggcttaac tctgggcgtg cagtgggtac gggcaggcta
      121 gagtgcagta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ctgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003205
VERSION     KHUT01003205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           99 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggctatc
       61 caagtcagac gtgaaaggcc tgggctcaac ccgggaactg cgtttgagac tgggtggctg
      121 gagtccggga gaggtgagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactgga ccggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003206
VERSION     KHUT01003206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggctttt
       61 taagtcagac gtgaaagccc taggcttaac ctaggaactg cgtttgatac tgaaaagcta
      121 gagtgtggaa gaggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccaacactga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003207             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003207
VERSION     KHUT01003207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggtggctcga
       61 caagtcggat gtgaaaactc caggctcaac ctggagacgc cactcgatac tgtcgtggct
      121 agagttcggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgga gatatcagga
      181 ggaacaccga tggcgaaggc agtgatctgg gccgatactg acactgaggc gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01003208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003208
VERSION     KHUT01003208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtta ctgggcgtaa agggtccgca ggcgggtctg
       61 taagtctgac gtgaaatctt ctggctcaac tagaagaggt cgttggaaac tacaggtcta
      121 gcatcccgca gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg cgcggatgga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003209
VERSION     KHUT01003209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggagcgta ggcggaacga
       61 caagtcagag gtgaaagccc ggggctcaac ctcggaattg cctttgagac tgtcgatcta
      121 gagtttggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccaatactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003210
VERSION     KHUT01003210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           96 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggtcatt
       61 ttagtcaggc gtgaaattcc cgggcttaac ctggggactg cgtttgatac tgggtgactt
      121 gagggtggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003211
VERSION     KHUT01003211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           81 g           58 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcgggttgg
       61 caagtcagag gtgaaatctc acagcttaac tgtgaaactg cctttgatac ttccaatctt
      121 gagttaaaga gaggttgatg gaatttcagg tgtagcggtg aaatgcgtag atatctgaaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ttttaactga cgctcaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003212
VERSION     KHUT01003212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c          101 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatggg
       61 acagtcagat gtgaaattcc cgggctcaac ctgggggcgg catttgagac gttttgtctg
      121 gagtgcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003213
VERSION     KHUT01003213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           95 g           52 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaaacta gaggctcaac ctctagcctg cagtgggtac gggcagactt
      121 gagtggtgta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg cacttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003214
VERSION     KHUT01003214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           50 c          104 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggcctgc
       61 tgcgtctgcc gtgaaattct ggggcttaac ttcggacgtg cggtggatac gggcaggcta
      121 gagtccggtt ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003215
VERSION     KHUT01003215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggttgca
       61 cgcgtttccg gtgccagctc gttgcttaac gacgggagtg ccgggaagac ggtgtgactg
      121 gaggacctca gagggacata gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaataccaat ggcgaaggca gtgtcctggg aggtagctga cgctaaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003216
VERSION     KHUT01003216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggtgaac
       61 acagttaggc gtgaaattcc cgggctcaac ctgggggctg cgtttaatac atgttcgctt
      121 gagttcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003217
VERSION     KHUT01003217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtttcg
       61 caagtcaggt gtgaaagccc agggctcaac cctggaagtg cacatgatac tacggagctt
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003218
VERSION     KHUT01003218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           48 c          102 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cgcgttgtcc gtgaaattcc ctggcttaac tgggggcgtg cgggcgatac gggcagactg
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003219
VERSION     KHUT01003219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccacg
       61 taagtcggat gtgaaagccc cgggctcaac tcgggaagtg cactcgatac tgcgtggctt
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cagatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003220
VERSION     KHUT01003220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           97 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtgggc
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tggccgactg
      121 gagagcagta agggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctgga ctgcttctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003221
VERSION     KHUT01003221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgta ggcggttggt
       61 taagatgggt gtgaaagctc ctggctcaac tgggagaggt cgctcatgac tggccgactg
      121 gagagcagta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ctgcatctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003222
VERSION     KHUT01003222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggttctt
       61 caagtctctc gtgaaatccc tcggcttaac tgaggacgtg cgggagatac tgaagggctt
      121 gagtccggaa gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003223
VERSION     KHUT01003223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          102 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtccgct gtgaaagccc ggtgcttaac gccggagggt cggtggatac tgccagtctt
      121 gaaggtgcta ggggaagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtcttctgga gcactcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003224
VERSION     KHUT01003224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggtggtctgc
       61 taagtcgtgt gtgaaatgcc ccggctcaac cggggaatgg cgcacgatac tggcaggctc
      121 gaggtcggga ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg actcactggc ccgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003225
VERSION     KHUT01003225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           75 g           61 t
ORIGIN      
        1 tacgaagggt ccaagcgtta tccggattta ttgggtttaa agggtgcgca ggcggggcct
       61 taagtcagtg gtgaaatctt gtagctcaac tacgaaactg ccattgatac tgaggttctt
      121 gaatgtagtt gacgttagtg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat cgcgaaggca gctaacgaag ctattattga cgctcaggca cgacagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003226
VERSION     KHUT01003226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           75 g           61 t
ORIGIN      
        1 tacgaaggat tcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactga
       61 taagtcagtg gtgaaagcct gttgcttaac aacagaactg ccattgatac tgccagtctt
      121 gagtatagtt gaggtaggcg gaatgtgtca tgtagtggtg aaatacttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttgctaaa ctataactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003227
VERSION     KHUT01003227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           95 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccgg
       61 taagtccgct gtaaaagtca agggctcaac ccttgaaagc cggtggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003228
VERSION     KHUT01003228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtccgta ggtggctcgc
       61 taagtcacac gtgaaatccc tcagcttaac tggggaactg cgtgtgagac tggggagctt
      121 gagggtagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctacttctga cactgaacga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003229
VERSION     KHUT01003229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           81 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggtggtcatg
       61 taagtcaaat gttaaagact ggggctcaac ctcagatttg catgtgatac tgcgtgactt
      121 gagaactgga gagggatctt gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaaa ggcgaaagca ggatcctggc cagcttctga cactcaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003230
VERSION     KHUT01003230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcttgc
       61 caagtcagac gtgaaagcca cgggctcaac ccgtgaagtg cgcttgatac tggtaggcta
      121 gagtctcgga gaggttggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaataccggt ggcgaaggcg gccaactgga cgaagactga cgctgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003231
VERSION     KHUT01003231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           77 g           60 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggcggatttg
       61 taagtcagag gtgaaactcc ggagcttaac tccggaactg cctttgatac tgcaagtctt
      121 gaatttgaga gagggtgatg gaataactgg tgtagcagtg aaatgcgtag atatcagtta
      181 gaacaccaat ggcgaaggca gtcacctatc tcataattga cgctaaagca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003232
VERSION     KHUT01003232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           37 c           70 g           68 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcttaa
       61 taagtcagcg gtgaaagttt ttggcttaac caaaaaattg ccattgatac tgttaggctt
      121 gaatttttgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactaac aaacgattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003233
VERSION     KHUT01003233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccatg
       61 caggtccgtt gtgaaaactc gaggcttaac ctcgagacgc cggtggaaac cgtgtggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003234
VERSION     KHUT01003234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           80 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcaggg gtgaaagacg gtagctcaac tatcgcagtg cccttgatac tgacaggctt
      121 gaatgaacta gaggtaggcg gaatgtgaca agtagcggtg aaatgtttag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactatg gttttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003235
VERSION     KHUT01003235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcgggtttt
       61 taagtcaggg gtgaaagcct ggagctcaac tccagaactg cccttgatac tgggaatctt
      121 gagtatggga gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggc ccattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003236
VERSION     KHUT01003236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          105 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggcggctggt
       61 taagtcctgt gtgaaagacc acggctcaac cgtgggggtg cacgggaaac tggccggctt
      121 gagtgcagga gaggggagcg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctccctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003237             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003237
VERSION     KHUT01003237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           47 t
ORIGIN      
        1 cacgtagggc gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 ttagtcgggt gtgaaaactc agggctcaac tctgagaggc cactcgatac tgccatgact
      121 cgagtccggt agaggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tagcgaaggc ggtgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003238
VERSION     KHUT01003238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           96 g           41 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgcg
       61 caagtcggat gtgaaacccc ggggctcaac cccgggcctg cacccgatac tgcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003239
VERSION     KHUT01003239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacagagggc ccgagcgttg cgcggaatca ctgggcttaa agcgtgcgta ggcggatcgt
       61 taagtgtttt gtgaaatccc acggctcaac cgtggaattg cagggcaaac tggcgatctt
      121 gaggtcggta ggggtcgtcg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgccaaa ggtgaagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003240
VERSION     KHUT01003240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           88 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcaggg gtgaaagcct ggagctcaac tccagaactg cccttgatac tggcaatctc
      121 gagtccggaa gaggtaagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003241
VERSION     KHUT01003241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           86 g           58 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgcttgta ggtggttttg
       61 taagttgggc gtgaaatctc cgggcttaac ttggagaggc cgtccaatac tgcgaggcta
      121 gaggttattc gaggaaagtg gaattcctgg tgtagtagtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctttctagg atatacctga cactgagaag cgacagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003242
VERSION     KHUT01003242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggattat
       61 taagtcagtg gtgaaatccc gaggctcaac ctcggaactg ccattgatac tattagtctt
      121 gaatgttgtg gaggtgggcg gaacgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca gcccgctacg caacgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003243
VERSION     KHUT01003243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttgtt
       61 cacgtcggat gtgaaagctc agggctcaac cctgagaggc cattcgatac gggacgacta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003244
VERSION     KHUT01003244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggccatc
       61 caagtccgat gtgaaagctc ggggctcaac cccgagtctg cattggaaac tgtatggctt
      121 gagtccggaa gaggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg ggtctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003245
VERSION     KHUT01003245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctgat
       61 taagtcagtg gtgaaataca gccgctcaac ggttgaggtg ccattgatac tgatcagctt
      121 gaaagaattg gaggctgccg gaatggatgg tgtagcggtg aaatgcatag atatcatcca
      181 gaacaccgat tgcgaaggca ggtggctacg atttatttga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003246
VERSION     KHUT01003246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcaatg
       61 taagtcgaac gtgaaagccc tcagcttaac tgaggaagtg cgtttgatac tgcatcgctt
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgacgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003247             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003247
VERSION     KHUT01003247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           95 g           49 t
ORIGIN      
        1 tacgtagggt cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtccgg
       61 taagtcaggt gtgaaatctc cacgctcaac gtggaggggc cacctgatac tgccgtgact
      121 tgagtctggt aggggagtgc ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003248
VERSION     KHUT01003248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          102 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgtgta ggcgggttgt
       61 caagtccgct gtgaaagccc gaggctcaac ctcggagggt cggtggatac tgacagtctt
      121 gaaggtgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gcattcttga cgctgagacg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003249
VERSION     KHUT01003249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           46 t
ORIGIN      
        1 tacggagggg gccagcgttg ctcggaatca ctgggcgtaa agggctcgta ggcggtctga
       61 taagtcctgt gtgaaatccc ttggcttaac caaggaactg cgccggatac tgtcggacta
      121 gaggccggga gagggaagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccggttctga cgctaaaaag cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01003250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003250
VERSION     KHUT01003250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtacgta ggtggttaga
       61 taagtcattt gtagaatccc cgggctcaac ctgggaatgt cggatgaaac tgtttgactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctgatactga cactgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003251
VERSION     KHUT01003251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           90 g           53 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctatc
       61 taagtagggt gtgaaagctc tcggctcaac cgaggaattg cgctctaaac tggatggctt
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccggt ggcgaaagcg gcacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003252             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003252
VERSION     KHUT01003252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           84 g           58 t
ORIGIN      
        1 tacgagaggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggtatga
       61 taagtctgtt gttaaatatc ctggctcaac caggaaaatg caatggaaac tattgttcta
      121 gagggtgaga gagagaagtg gaattccagg agttagcggt caaatgcgta gatctctgga
      181 ggaacaccga tggcgaaggc agcttcttgg ctcatttctg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003253
VERSION     KHUT01003253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           97 g           49 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtctgac gtgaaatgta ccggttcaac cggggcaccg tgtcggaaac tgcctggctt
      121 gaggtcgagg gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctatc tcgattctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003254             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003254
VERSION     KHUT01003254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcatt
       61 taagtttggt gtttaagccc ggggctcaac cccggatcgc actgaaaact gggtgacttg
      121 agtgcgggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggac cgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003255             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003255
VERSION     KHUT01003255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           37 c           87 g           57 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggttcgta ggcggtaatt
       61 taagtcaggt gtgaaaggcg caggctcaac ctgtgtaagc acttgaaact ggattacttg
      121 agttaaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aataccagtg gcgaaggcga ctttctggac ttatactgac gctgaggaac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003256
VERSION     KHUT01003256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           89 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcattc
       61 taagtcggat gtgaaatccc tcggctcaac cgaggacgtg catccgaaac tggaacgctc
      121 gagtatggaa gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003257
VERSION     KHUT01003257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          104 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctggg
       61 taagtgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcagac tgcttggctt
      121 gagcatagta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003258
VERSION     KHUT01003258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagtccgta ggcggcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaactg cgctccaaac taccgtgcta
      121 gaggaaccta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg agttttctga cgctgaggga cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003259
VERSION     KHUT01003259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttatc
       61 cacgtcggat gtgaaagctc agggctcaac cctgggatgc cattcgatac gggatggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003260
VERSION     KHUT01003260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           91 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttt
       61 taagtccggg gtgaaagccc gttgctcaac aacggaactg ccctggatac tggaaggctt
      121 gagtacagac gaggttggcg gaatggacgg agtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctgactagg ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003261
VERSION     KHUT01003261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           93 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggctttt
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaatac tgagaggcta
      121 gagtgcggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtaaacg gctttctgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003262
VERSION     KHUT01003262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggattca ctgggcttaa agggtgcgta ggcggcttgc
       61 caagtagggt gtgaaagccc ccggcttaac cggggaattg cgccctaaac tggcgagctg
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003263
VERSION     KHUT01003263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          107 g           39 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccgac
       61 cgcgtcgggt gtgaaagccc tccgctcaac ggaggagagt cgctcgaaac gggtgggctg
      121 gaggcaggga gaggtcggtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactgga cctgttctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003264             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003264
VERSION     KHUT01003264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           97 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatgag
       61 cacgtctgtc gctgaaatcc cccggctcaa ccggggaacg ggcgcggata cggctcatct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003265
VERSION     KHUT01003265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           82 g           56 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggccgt
       61 taagtcaggg gtgaaagtct gcggctcaac cgtagaactg cccttgatac tggcggcctt
      121 gagttatggt gaagtagcca gaatatgtgg tgtagcggtg aaatgcatag atatcacatg
      181 gaataccgat tgcgaaggca ggctactaac catatactga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003266
VERSION     KHUT01003266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcgatg ttgaaagttt gccgcttaac ggtaaaaatg gcaacgatac tggcgggctt
      121 gagtacagac gaggtaggcg gaattgataa tgtagcggtg aaatgcatag atattatcag
      181 gaactccgat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003267
VERSION     KHUT01003267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtgggg
       61 taagtttgag gtgaaagctc cgggctcaac ccggaaaatg ccttgaaaac tatccggcta
      121 gagggttgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atctcgagag
      181 gaacaccagt ggcgaaggcg ggtctctgga caacacctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003268
VERSION     KHUT01003268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggtggtttgt
       61 tatgtcggat gtgaaagccc tcggctcaac tgaggaagtg catccgaaac tggcaagcta
      121 gagtatgtaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg acaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003269
VERSION     KHUT01003269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctca
       61 taagtccggg gtgaaagccc gttgcttaac aacggaactg ccctggatac tgcgaggctt
      121 gagtacagac gaggttggcg gaatggaccg agtagcggtg aaatgcatag atacggtcca
      181 gaaccccgat tgcgaaggca gctgactagg ctgttactga cgctgaggca cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003270
VERSION     KHUT01003270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          104 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggtg
       61 ttagcgtgcg gtgaaagccc gaggctcaac ctcgggtcgg ccgtgcgaac tgcatcgctt
      121 gagcagagta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctctggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003271
VERSION     KHUT01003271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcaagg
       61 caagtcggat gtgaaatccc gtggctcaac catggacgtg cactcgaaac tgcctagcta
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacacctgt ggcgaaggcg gccttctgga cgatgactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003272             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003272
VERSION     KHUT01003272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaggta ggtggtctga
       61 tttgtctgga ggtgaaagcc ttgggcttaa cccaagaagt gccccggaaa cggtcagact
      121 ttgagtcttg gagagggttg cggaattccc ggtgtagcgg tgaaatgcgt agagatcggg
      181 aggaacacca gaggcgaagg cggcaacctg gacaagcact gacactcaac tgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01003273             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003273
VERSION     KHUT01003273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           39 c           80 g           57 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggcatga
       61 caagttatca gtgggaaatc ggggctcaac ctcgtggaag ctggtaatac tattatgctt
      121 gagtgcagga ggggttaatg gaactccatg tggagcggta aaatgtgacg atatatggaa
      181 gaacaccaat aaggcgaagg caattaacta ttctgctact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01003274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003274
VERSION     KHUT01003274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           86 g           54 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgta ggtggtttgt
       61 taagttgggt gttaaatctc tcggctcaac tgagaggtgt cgctcaaaac tagcagactg
      121 gaggacggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gccttctgga ccgctcctga cactccaacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003275
VERSION     KHUT01003275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           92 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcttgta ggtggccaac
       61 taagtcagac gtgaaatccc tcggcttaac cggggaactg cgtctgatac tggatggcta
      121 gagattggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaacactga cactgaaaag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003276
VERSION     KHUT01003276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           80 g           57 t
ORIGIN      
        1 tacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtctgct gttaaagctt gaagcttaac ttcaaaaatg cagtagaaac ttttaaactt
      121 gagggtggta ggggttgagg gaatttccag tggagcggtg aaatgcgtag atcttgggaa
      181 gaccaccaat ggcgaaggca ctcgactggg ccactactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01003277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003277
VERSION     KHUT01003277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcttgt
       61 taagtagggt gtgaaatccc ccggctcaac cggggaattg cgccctaaac tggcgagctg
      121 gagtgagata ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccagt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003278             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003278
VERSION     KHUT01003278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgcgta ggcgggccga
       61 taagtcggtt gtgaaatccc tgagctcaac tcaggaactg catccgaaac tgtcggtcta
      121 gagtagtgga gagggatggc ggaattccgg gtgtagaggt gaaattcgta gatatccgga
      181 ggaacaccgg tggcgaaggc ggcctcctgg acacttactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003279
VERSION     KHUT01003279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggattta ttgggtgtaa agggtgcgta gacggcatgt
       61 taagtccgct gttaaatttc ccagctcaac tgggagccag cggtggatac tgatgtgcta
      121 gaggatggaa gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatatctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003280
VERSION     KHUT01003280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggttctg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcaaggctt
      121 gagtgctgga gaggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctatctgga cagtaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003281
VERSION     KHUT01003281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtctcg
       61 taagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cactcgaaac tgcgaggcta
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cagatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003282
VERSION     KHUT01003282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggttata
       61 taagtccatg gtgaaagacg gcagcttaac tgtcgcagtg ccattgatac tgtatgactt
      121 gaataaggat gaggttggcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccaat tgcgaaggca gctgactaga cctttattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01003283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003283
VERSION     KHUT01003283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttttc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tggggaactg
      121 gagtgcagga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ctgcaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003284
VERSION     KHUT01003284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           89 g           59 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ctgggtttaa agggtgcgta ggcgggctta
       61 taagtctggg atgaaatacc gatgctcaac gtcggtgccg ttctggatac tgtaggtctt
      121 gagagatgtt gaggtggctg gaatgagtgg tgtagcggtg aaatgcgtag agatcactca
      181 gaacaccgat tgcgaaggca ggtcaccggg cattatctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003285
VERSION     KHUT01003285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggccaac
       61 tcagtcagac gtgaaatccc tcagctcaac tggggaactg cgtctgatac tagatggctc
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003286
VERSION     KHUT01003286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           86 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggaactg
       61 taagtctggt ttgaaagcag gtggcttaac catctaatgt ggctggaaac tgtggttctt
      121 gaatgatttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg agttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003287
VERSION     KHUT01003287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           36 c           81 g           61 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggtggtttgg
       61 taagttagat gtgaaagccc aaagcttaac tttggaattg catttaaaac tgctggactt
      121 gaagatcgaa gaggtaagca gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaataccgat ggcgtaggca gcttactggt cgatacttga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003288
VERSION     KHUT01003288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           46 c           79 g           52 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggcggtcttg
       61 aaagttagaa gtgaaagccc tgggcttaac ccaggaattg cttttaaaac tccaagacta
      121 gagtactgga gaggttagcg gaattccaag tgtagcagtg aaatgcgtag atatttggag
      181 gaacaccaat ggcgtaggca gctaactgga cagtaactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003289
VERSION     KHUT01003289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtctgg
       61 caagtgaaag gtgaaatccc tcggctcaac tgaggaactg cctttcatac tgcttgactt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003290
VERSION     KHUT01003290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgagaa gtgaaagctc cgggctcaac ccggaaattg cttttcatac tggcagactt
      121 gagggtggca gaggtttgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccat ggcgaaggca gcaaactggg ccacatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003291
VERSION     KHUT01003291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           46 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccgaattta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 taagttgggc gtgaaagctc ccggctcaac tgggagaggt cgtccaaaac tgccagacta
      121 gaggacggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccgtgcctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003292
VERSION     KHUT01003292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           40 a           64 c          115 g           34 t
ORIGIN      
        1 tacgtggggt ccgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggcggcg
       61 tgggtccggc gtgaaagccc ccggctcaac cggggaaggt cgtcggggac cgcgccgctt
      121 gagggcggca ggggccggtg gaatgcctgg tgtagtggtg aaatgcgtag agatcaggcg
      181 gaacacccgt ggcgaaggcg gccggctggg ccgtccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003293
VERSION     KHUT01003293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           85 g           50 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggccaat
       61 taggtgaggt gtgaaatccc ccggcttaac cgaggaatgg cgcttcaaac cgattggcta
      121 gaggaagata gaggaaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctttctggg tcttttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003294
VERSION     KHUT01003294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           96 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacca tgagcttaac tcatggcttg ctttcgatac gggcagactg
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003295
VERSION     KHUT01003295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcaggg gtgaaaggcg gtagcttaac tatcggagtg cccttgatac tgacgggctt
      121 gaatgcagct gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactaaa gtgcgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003296
VERSION     KHUT01003296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           91 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtgggattc
       61 taagtctggt ttgaaagccg gtggctcaac catcggatgt ggctggaaac tggggttctt
      121 gaatgggttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagccgg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003297
VERSION     KHUT01003297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctgcg
       61 caagtcggat gtgaaatccc atggctcaac catggaagtg cacccgaaac tgcgtagcta
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003298
VERSION     KHUT01003298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaactg cctctgatac tggtcatctt
      121 gagtgaggta ggggcgtgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcgcgctgga ccttaactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003299
VERSION     KHUT01003299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           45 c           99 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatgtt
       61 ttagtcaggc gtgaaagtcc tgggctcaac ctggggattg cgtttgatac ggggcatcta
      121 gagttgggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003300
VERSION     KHUT01003300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           78 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggttgatagg
       61 taagtcagtg gtgaaatact ccgactcaat cggagaactg ccgttgatac tatctatctt
      121 gaatacggtg gaggcgggcg gaatgcgtca tgtagtagtg aaatacttag atatgacgca
      181 gaacacctat tgcgaaggca gctcgctaca ccggcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003301
VERSION     KHUT01003301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c           98 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggtggttcgt
       61 tgcgtccgag gtgaaatctc ccggctcaac tgggaggggt cttcggatac gggcggactt
      121 gaggagagca gaggtaggcg gaattctggg tgtagtggtg aaatgcgtag atatccagag
      181 gaacaccagt ggcgaaggcg gcctactggg ctccacctga cactgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003302             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003302
VERSION     KHUT01003302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           94 g           43 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttgag
       61 caagtctgat gtgaaagccc cgggcccaac ccgggaatgg catcggaaac tgcccggcta
      121 tagagggttg gaggggggac tggaattccc ggtgtagcag tgaaatgcgt agatatcggg
      181 aggaacgcca gtggcgaagg cgagtccctg gacaacacct gacactgagg cacgaaagct
      241 tggggagcaa acagg
//
LOCUS       KHUT01003303             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003303
VERSION     KHUT01003303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           82 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggttatg
       61 taagtcagca agtgaaattc cacagcttaa ctgtagggac tgcttttgat actgcatgac
      121 ttgaaccaag atgaggtagg cggaatgtgg catgtagcgg tgaaatgcat agatatgcca
      181 tagaacaccg attgcgaagg cagcttacta gacttgtgtt gacgctgagg cacgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01003304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003304
VERSION     KHUT01003304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           49 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggtggtttgt
       61 taagtcaggg gtgaaatccc gcggcccaac cgtggaactg cctttgatac tggcgaactt
      121 gagtgcgtga ggggtagggg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccaga gccgaagggg ccctactggt gcgcaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003305
VERSION     KHUT01003305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgc
       61 taagtcttgc gtgaaatctc ggggctcaac cccgagcggt cgtgggaaac tggcaggcta
      121 gagtgcggaa gaggagaatg gaattcctag tgtagcggtg aaatgcgcag atattaggaa
      181 gaacacccgt ggcgaaggcg gttctctggg acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003306
VERSION     KHUT01003306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggacat
       61 taagtcaggg gtgaaagacg gttgctcaac aatcgcagtg cctttgatac tggtgttctt
      121 gggtaacctt gaggtaggcg gaatgggtgg tgtagcggtg aaatgcatag ataccaccca
      181 gaactccgat tgcgaaggca gcctactgga ggtcaaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003307
VERSION     KHUT01003307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           52 c          104 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggcgcgca ggtggtgtgc
       61 taagtctgcc gtgaaatctc ccggctcaac tgggaggctg cggtggagac tggcacgctt
      121 gaggtaccta gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ggtgacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003308
VERSION     KHUT01003308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacgaggggg gctagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcggacctt
       61 taagttggat gtttaacccc ggggcccaac cccggatcgg catccaaaac tggaggtctt
      121 gaatagtgga gaggtcgatg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccaat tgcgaaggca gtcgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003309
VERSION     KHUT01003309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggagcgta ggcgggctat
       61 caagtctggg gtgaaatacc acggctcaac cgtggaactg cctcagatac tgacggcctc
      121 gagggagata ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaagcg gcgtgctgga tctcttctga cgctgaggct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003310
VERSION     KHUT01003310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           86 g           49 t
ORIGIN      
        1 gacgagcctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtag
       61 aaagttgggt gtgaaatccc tcagcttaac tgaggaaggg cgcccaaaac tactatgctc
      121 gagggagata gaggagtgcg gaacttaggg tggagcggtg aaatgcgttg atatcctagg
      181 gaacaccggt ggcgaaagcg gcactctgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003311             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003311
VERSION     KHUT01003311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtatgta ggcggatcgt
       61 taagtcgggg gtgaaatccc gaggctcaac ctcggaactg ccttcgatac tggcgatctt
      121 cgagtccggg agaggtgagt ggaactccga gtgtagaggt gaaattcgta gatattcgga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgaaat acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003312
VERSION     KHUT01003312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           77 g           62 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggccttg
       61 taagtcagtg gtgaaatcct gtcgcttaac gatagaattg ccattgatac tgcagggctt
      121 gagtacagtt gccgttggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctgacgaaa ctgtaactga cactgaggct cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003313
VERSION     KHUT01003313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctcgg
       61 acagtcggat gtgaaaatcc ggggctcaac cccggacctg catccgatac ttccgggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003314             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003314
VERSION     KHUT01003314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           94 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcatgta ggcggatagg
       61 aacgtcggat gttgaaagcc cccggctcaa ccggggaagt ggcaccgaaa cgacctgtct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttttctg acgctgagat gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003315
VERSION     KHUT01003315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgagcgta ggcggcccga
       61 taagtctgct gtgaaagtca acggctcaac cgttgaaagc cggtggatac tgtcgggcta
      121 gagttcggaa gaggcaagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacccat agcgaaggca gcttgctggg acgatactga cgctaaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003316
VERSION     KHUT01003316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           94 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgtcg
       61 taagtcgggt gtgaaatctc ggggctcaac tccgaaattg cattcgatac tgcggtgctt
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003317
VERSION     KHUT01003317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           95 g           39 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtgcccc gtgaaatccc acggctcaac cgtggaaccg cgggacatac tggcggtctt
      121 gagagaccta ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtcttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003318
VERSION     KHUT01003318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           41 c           92 g           57 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttgga
       61 taagtgtggt gtgaaatctc ccggcttaac tgggagggtg cgctacaaac tgtctgacta
      121 gagtgtggga tgggtaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcttactgga ccataactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003319
VERSION     KHUT01003319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggtggatgga
       61 caagtcggaa gtgaaatctt gaggctcaac ctcgaggctg ctcccgaaac tgttcgtcta
      121 gggactggta gaggctggta gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccaggtccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003320
VERSION     KHUT01003320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 gacgaaggtg gcaagcgtta ttcggattta ctgggcataa agcgcgcgta ggcggcttgg
       61 taagtcgggg gtgaaagccc ggagctcaac tccggaattg ccttcgatac tgccgagctt
      121 gagtgcagga ggggtgactg gaactcaagg tgtagcggtg aaatgcgtag atatcttgag
      181 gaacaccgga ggcgaaggcg agtcactgga ctgccactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003321
VERSION     KHUT01003321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c          102 g           43 t
ORIGIN      
        1 gacagaggtg gcaagcgttg tccggaatta ctgggcgtaa agagcgcgta ggtggctaga
       61 taagtccggt gtgaaatcct tcggcttaac cggggaatgg catcggggac tgtttagcta
      121 gaggacggga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gctctctgga acgtccctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003322
VERSION     KHUT01003322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtgatc
       61 tttgtcagag gtgaaagccc ggggctcaac tccggaattg cctttgaaac gggatcgcta
      121 gagtccgaga gaggatggcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggc tcggtactga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003323
VERSION     KHUT01003323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtga
       61 caagtcactt gtgaaatctc cgggctcaac tcggaaaggc caagtgatac tgtcatgcta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003324
VERSION     KHUT01003324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           95 g           51 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggcggcttgt
       61 taagtcaggg gtgaaagttt tcggcccaac cggaaaattg cctttgatac tggcgagctt
      121 gagtgcgtga gggatggggg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccaga gccgaagggg ccccattggt acgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003325
VERSION     KHUT01003325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           99 g           45 t
ORIGIN      
        1 cacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccgt
       61 cacgtcggga gtgaaaactc ggggcttaac cccgagcctg cttccgatac gggcggacta
      121 gaggtaggta ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003326
VERSION     KHUT01003326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtt
       61 caagtccggg gtgaaatccc acagctcaac tgtggaactg ccttggatac tgggcacctc
      121 gagggtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccacatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003327
VERSION     KHUT01003327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           89 g           47 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgac
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaatgg cttggcatac tggtcgtctt
      121 gagcggtcta ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaaaaca gctcgctggg gacctgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003328
VERSION     KHUT01003328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           76 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggaccag
       61 taagtcagtg gtgaaatctc ccggctcaac cgggaaatgg ccattgatac tgctggtctt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca ggttactact aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003329
VERSION     KHUT01003329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           91 g           45 t
ORIGIN      
        1 tacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagcgt
       61 taagtgcctc gtgaaatccc acggcttaac cgtggaacgg cttggcatac tggcgttctt
      121 gagcggtcta ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgcccat ggtgaaaaca gctcgctggg gacctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003330
VERSION     KHUT01003330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggcttt
       61 taagttaggt gtttaatccc ggggcccaac cccggacagg cacttaaaac tgggagtctt
      121 gaatagggga gaggccagcg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccagt tgcgaaggcg gctggctgga cccatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003331
VERSION     KHUT01003331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           96 g           41 t
ORIGIN      
        1 aacgtaggac acgagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggcatgg
       61 taagtctgat gtgaaagctc ccggctcaac cgggagagga cattggagac tgccaagctc
      121 gaggcagata gagggatgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcatcctggg tctgacctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003332
VERSION     KHUT01003332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggaggt
       61 taagtcaggg gtgaaagacg gtcgctcaac gatcgcagtg cccttgatac tggccttctt
      121 gggtgacctt gaggtaggcg gaatgggcgg tgtagcggtg aaatgcatag ataccgccca
      181 gaaccccgat tgcgaaggca gcctactggg ggtttaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003333
VERSION     KHUT01003333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          100 g           43 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggctttt
       61 caagtggctc gtgaaagact tcggctcaac cgggggaggt cgggtcagac tgagaggcta
      121 gaggatgtca gagggacacg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaggcg gtgtcctggg acgtacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003334
VERSION     KHUT01003334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           85 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggaaat
       61 taagtcgggg gtgaaagacg gttgctcaac aatcgcagtg ccttcgatac tggttttctt
      121 gggtgaagtt gaggtaggcg gaattcaagg tgtagcggtg aaatgcatag ataccttgaa
      181 gaactccgat tgcgaaggca gcctactgga ctttcaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003335
VERSION     KHUT01003335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacga
       61 caagtccggc gtgaaaggct cgggctcaac ccgagacggc cgttggaaac tgtcgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gctctctggt cgacctctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003336
VERSION     KHUT01003336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttata
       61 tgagtggtat gtgaaagccc tgggcttaac ctaggaagtg catcccaaac gatataactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003337
VERSION     KHUT01003337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggcttg
       61 taagtcagtg gtgaaatctc cgtgcttaac atggaaactg ccattgatac tataggtctt
      121 gaattatctg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca ggtacattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003338
VERSION     KHUT01003338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          107 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctct
       61 caaggctcgt gtgaaagtcc ggggctcaac cccggatcgg caggggcgac tgagaggctg
      121 gagctctgta gaggcgggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003339
VERSION     KHUT01003339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          102 g           44 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggattga ctgggcgtaa agggcgtgta ggcggttggt
       61 caagtctggt gtgaaagccc ggggctcagc tccggaagtg catcggaaac tagccagctg
      121 gagcgcgata gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccctctgga tcgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003340
VERSION     KHUT01003340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          106 g           37 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 cgcgtccggg gtgaaatcct ccggctcaac cggggaaggg cctcggatac gggcggcctg
      121 gagtggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003341
VERSION     KHUT01003341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcggc
       61 tatgtcggat gtgaaagcct cgggcttaac ccgagaaggg catccgaaac tggccggctt
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003342
VERSION     KHUT01003342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           75 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattgg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tatcagtctt
      121 gaataccgtg gaggccagta gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaataccaat tgcgaaggca gctggctacg cgaatattga cactcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003343             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003343
VERSION     KHUT01003343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           41 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatctc
       61 gtaagtcgga agtgaaatgt ggctgctcaa cggccacgct gctgccgata ctgcggatct
      121 ggggaccggt agaggctggt ggaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaacacccg tggcgaaggc ggccagctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003344
VERSION     KHUT01003344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggcctga
       61 caagtctgat gtgaaatccc tgggctcaac ccagggcctg catcggaaac tgtcgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccattactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003345
VERSION     KHUT01003345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           94 g           42 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttag
       61 caagtcgggc gtgaaagccc tcggctcaac cgaggaagtg cacccgaaac tgctgagctt
      121 gagtgccgga gaggatggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003346
VERSION     KHUT01003346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           75 g           63 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagcct acagcttaac tgtagaactg ccattgatac tgatgatctt
      121 gagtatgatt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactaag tcataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003347
VERSION     KHUT01003347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtttg
       61 aaagtctgcg gtgaaatcct ccagcttaac tggagaagtg ccgtggatac tgcaagcctc
      121 gaggagggta ggggcatctg gaacagtagg tggagcggtg aaatgcgttg atatctactg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003348
VERSION     KHUT01003348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           82 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcctga
       61 caagttgggt gtgaaatacc tcggctcaac cgaggaacag cgctcaaaac tgtcaggctt
      121 gagggagata gaggtgagcg gaacttaagg tggagcggtg aaatgcgttg atatcttaag
      181 gaacacccgt ggcgaaagcg gctcactgga tctcttctga cgctcatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003349
VERSION     KHUT01003349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           94 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtccgt
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaacgg catctgatac tggcgggctc
      121 gaggatggca ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccatatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003350
VERSION     KHUT01003350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 tacgaaccgt acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccacg
       61 taagtgagat gtgaaatccc tcggctcaac cgaggaagtg catttcatac tgcatggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003351
VERSION     KHUT01003351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           88 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatttg
       61 ttagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgcaagtctc
      121 gagtccgata gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactgga tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003352
VERSION     KHUT01003352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatca ctgggcttaa agcgttcgta ggcgggtctg
       61 taggcgcgtt gtgaaagccc tcggctcaac cggggaactg cttcgcgaac cacagatctt
      121 gagtcaagta ggggcgatcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccgaa ggtgaagaca ggtcgctggg cttgtactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003353
VERSION     KHUT01003353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           85 g           51 t
ORIGIN      
        1 tacggaggga gctagcgtta ttcggaaata ctgggcgtaa agcgcgcgta ggcggtatca
       61 taagtcagag gtgaaatccc agggctcaac cttggaactg cctttgaaac tgtggcacta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003354
VERSION     KHUT01003354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           95 g           49 t
ORIGIN      
        1 tacggggggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgtgca ggcggtcttg
       61 taagtctgat gtgaaagccc cgggcttaac ccgggaagtg cactggatac tgcaagactt
      121 gagttcggga gaggagagtg gaattcctgg agtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003355             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003355
VERSION     KHUT01003355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           43 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcgggt gtgaaaactc agggctcaac cctgagacgc cacctgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003356
VERSION     KHUT01003356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccga
       61 taagtaggac gtgaaattcc gtggcttaac cacggaactg cgttctagac tgtcaggctt
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003357
VERSION     KHUT01003357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgaa
       61 taagtctgac gtgaaatctg gaggctcaac ctccagctgt cgttggaaac tattcggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003358
VERSION     KHUT01003358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           86 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggttcag
       61 taagtctttt gtgaaagctc ccggctcaac tgggagaggc caagggaaac tactggacta
      121 gagtgtggta ggggatactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctat ggcgaaagca ggtatctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003359
VERSION     KHUT01003359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           76 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggttctt
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cctttgatac tgaagaactt
      121 gaatacactt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaaa gtgtcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003360
VERSION     KHUT01003360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgatt
       61 taagtagggt gtgaaatccc ccggctcaac cggggaactg cgctctaaac tggatcgctc
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003361
VERSION     KHUT01003361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccagc
       61 taagtcagac gtgaaatccc tcggcttaac cggggaactg cgtctgatac tggatggctc
      121 gagtgtggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccactactga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003362
VERSION     KHUT01003362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctaat
       61 tatgtcaggt gtgaaagccc tcggcctaac cgaggaagtg cgcctgaaac tgattagctt
      121 gagtacgaga gaggaggacg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gttctctgga tcgatactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003363
VERSION     KHUT01003363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           94 g           40 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcggg
       61 caagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tgcctgtctt
      121 gagccagcta ggggcgaccg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaaa ggtgaaaaca ggtcgctggg gctgagctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003364
VERSION     KHUT01003364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggttata
       61 caggtccgct gtgaaaactg gaggcttaac ctccagacgt cggtggaaac cgtataacta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003365
VERSION     KHUT01003365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcatgg
       61 taagtctcgt gtgaaagccc tgggctcaac ccaggaagtg cgcgagatac tgccgtgctg
      121 gagtgatgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga catttactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003366
VERSION     KHUT01003366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggccttg
       61 taagtcggag gtgaaatcca ctggctcaac cagtgacgtg ccttcgaaac tgcttggctt
      121 gagtatggca gaggagagcg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgtaggcg gctctctggg ccaatactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003367
VERSION     KHUT01003367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          104 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggcggct
       61 taagtcgggt gtgaaagccc ggggctcaac cccgggatgc cgcccgagac tgggtcgctt
      121 gaggtcggaa gaggggagtg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccgattctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003368
VERSION     KHUT01003368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           89 g           45 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaattg cgctctaaac taccgtgctc
      121 gaggaagcta gaggtgagcg gaactgaagg tggagcggtg aaatgcgttg atatcttcag
      181 gaacaccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003369
VERSION     KHUT01003369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 caagtttggt gtgaaatcct agggctcaac cctggaactg cactgaatac tgctttgcta
      121 gagtactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003370
VERSION     KHUT01003370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcctga
       61 tcagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgtcaggcta
      121 gaaggcagga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcttga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003371
VERSION     KHUT01003371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           85 g           49 t
ORIGIN      
        1 gacgagccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac tgccgtgctc
      121 gaggaagata gaggtaagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacacctgt ggcgaaagcg gcttactggg tcttttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003372
VERSION     KHUT01003372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcggtcgtg
       61 taagtagcgc gtgaaagcgc ccggcttaac cgggcgagga cgtgctgtac tgcacgactg
      121 gagatgggta gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacacctga ggcgaaggcg actcgctggg cccgttctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003373
VERSION     KHUT01003373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          105 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgatg
       61 cacgcgtggg gtgaaagtcc ggggctcaac cccggatcgg ccttgcggac ggcattgctt
      121 gagcatggta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ccattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003374
VERSION     KHUT01003374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           84 g           52 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgtgcgg
       61 aaagttgggt gtgaaatacc tcagcttaac tgaggaactg cattcaaaac taccgtgcta
      121 gaggaagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003375             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003375
VERSION     KHUT01003375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           50 t
ORIGIN      
        1 cacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcga
       61 taagtcgggt gtgaaaactc tgggctcaac tcagagacgc cactcgatac tgtcgtgact
      121 ggagtctggt aggggagtgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtactg acactgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003376             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003376
VERSION     KHUT01003376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           97 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattga ctgggcgtaa agcgcacgta ggtggttggt
       61 tacgtggtgt gtgaaagctc agggcttaac cctgataggc catgccaaac gaaccgactc
      121 gaggctgagc agaggcgggt ggaattgccg gtgtagtggt gaaatgcgta gagatcggca
      181 ggaacaccaa gggggaaccc agcccgctgg gccagtcctg acactgaggt gcgacagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003377
VERSION     KHUT01003377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggtcggt
       61 caagtctggg gtgaaatctt tcggctcaac cggaaaagtg ccccggatac ggatcggctg
      121 gagggaggta ggggtcgacg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gtcgactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003378
VERSION     KHUT01003378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 cacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtttgt
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaaaac tggcagacta
      121 gagggtggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctggg ccacccctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003379             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003379
VERSION     KHUT01003379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          104 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctcgg
       61 taagcgggcg gtgaaagtcc agggctcaac cccggatctg ccgtgcggac tgccgtggct
      121 cgagcactgt agaggcaggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcctgctgg gcagttgctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003380
VERSION     KHUT01003380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           64 c          104 g           32 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggagacg
       61 gacgtgccgt gtgacagctc accgctcacc ggtgaaaggc cacggtacac gacggctctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01003381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003381
VERSION     KHUT01003381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggttcag
       61 taagtctgct gtgaaagtcc aaggctcaac cttgggatgc cggtggatac tgctgagcta
      121 gagtacggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacacctat tgcgaaggca gctcgctggg acgtcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003382
VERSION     KHUT01003382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgtg
       61 caagtcggat gtgaaattcc agggctcaac cctgaaaagc cattcgatac tgctcggcta
      121 gaggcaggta ggggaaagtg gaatacctgg tgtagcggtg aaatgcgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg gctttctggg cctcgcctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01003383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003383
VERSION     KHUT01003383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctctt
       61 caagtcaggc gtgaaagccc cgggctcaac tcgggaactg cgcttgatac tgtcgagcta
      121 gagtgtggaa ggggcgattg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccaga ggcgaaggcg gatcgctggg ccaacactga cgctgagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003384
VERSION     KHUT01003384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggaccat
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cctctgatac tggcggtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003385
VERSION     KHUT01003385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggctgattcg
       61 ttagtcgggt gtgaaatccc tgggctcaac ccaggaactg catccgaaac tgcgagtcta
      121 gagtgtcgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003386
VERSION     KHUT01003386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggcccat
       61 taagtcggct gtgaaatcct ggggctcaac cccagaactg cagtcgatac tgatgggctt
      121 gaggtaggta gaggagagtg gaattcccag tgtagcggtg gaatgcgcag atattgggag
      181 gaacaccaat ggcgaaggca gctctctggg ccttacctga cgctgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003387
VERSION     KHUT01003387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtttgt
       61 cgcgtccgct gtgaaaaccc gaggctcaac ctcgggcctg cagtgggtac gggcagacta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccgcaactga cactgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003388
VERSION     KHUT01003388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacga
       61 caagtgggga gtgaaattcc ggggctcaac cctggacctg ctttccaaac tgtcgacctg
      121 gggaccggta gaggctggta gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003389
VERSION     KHUT01003389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           94 g           41 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccacg
       61 caggtcgggt gtgaaagccc ttggctcaac caaggaagtg cactcgaaac cgcatggctc
      121 gagtcccgga gaggaaggtg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggc ggcgaaagcg gccttctgga cgaggactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003390
VERSION     KHUT01003390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           97 g           40 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 aaagtcggat gtgaaaacct ggggctcaac cccaggcgtg cacccgatac tcccgggctg
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003391
VERSION     KHUT01003391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctaga
       61 tgtgtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgtctggctt
      121 gaggccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003392
VERSION     KHUT01003392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           90 g           45 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgcgta ggcggttgat
       61 caagttggat gtgaaagccc tcggcttaac cgaggaagtg catccaaaac tgaacagcta
      121 gagtactaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003393             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003393
VERSION     KHUT01003393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           43 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gcaagtcaga ggtgaaatct cactgctcaa cggcgagact gcttctgaga ctgcggatct
      121 ggagaccggt agaggctggt ggaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaacacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003394
VERSION     KHUT01003394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           88 g           47 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggtaact
       61 caagtcagat gtgaaatatc cgggcttaac ccggagaggt catttgaaac tgagtaacta
      121 gagtacagca gaggatagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctggg ctgaaactga cgctgaggcg cgaaggctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003395
VERSION     KHUT01003395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggctag
       61 taagtcagag gtgaaatctc caagcttaac ttggaaactg cccctgatac tattagtctt
      121 gaatatcgat gaggcgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcgctaac cgattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003396
VERSION     KHUT01003396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtcttc
       61 tacgtttcca gtgacaggtg gtggctcaac tgccacaggt ctgggaaaac gggttgactt
      121 gaggacgaga gagggatgcg gaattccagg tggagcggtg aaatgcgtag agatctggaa
      181 gaacaccaat ggcgaaggca gcatcctgga tcgtttctga cgctccggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003397
VERSION     KHUT01003397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgcacgta ggcggcttgg
       61 caagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgaaac tgctaggcta
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003398
VERSION     KHUT01003398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          103 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccgat
       61 caagtgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcaaac tggttggctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003399
VERSION     KHUT01003399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           51 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggttttt
       61 taagtccttt gtgaaatccc ggagctcaac tccggatatg cagtggatac tggaagacta
      121 gagaccggta gaggctagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gctagctggg ccgggtctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003400
VERSION     KHUT01003400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggctgag
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaattg cattcgatac tgttcggcta
      121 gagtacggta gagggaggta gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaataccggt ggcgaaggcg gcctcctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003401
VERSION     KHUT01003401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           87 g           49 t
ORIGIN      
        1 gacgaacctg acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 tcagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac taccgtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003402
VERSION     KHUT01003402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcaagta ggcggttgca
       61 caagtcatgt gttaaagccc ctggctcaac tgggggaggt catgtgagac tgtgcgactt
      121 gagggcaaca gaggcatgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gcatgctggg ttgcgactga cgctgagttg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003403
VERSION     KHUT01003403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 aaagtcagat gtgaaatccg ggggctcaac ctccgaatag catccgatac tcccatgcta
      121 gagaactgga gggggatctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacactagt ggcgaaggcg agatcctgga cagtatctga cactgatgca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003404
VERSION     KHUT01003404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggcttct
       61 taagtctgac gtgaaagccc agggctcagc cttggaagtg cgttagaaac tgagaagctt
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003405
VERSION     KHUT01003405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          100 g           40 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcgggtgcg
       61 cgcgtggggt gtgaaaccag gtggcttaac tgcctgtcgc catcccatac ggcgcgcctg
      121 gagccatcca gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgactggg gatgtgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003406
VERSION     KHUT01003406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           90 g           43 t
ORIGIN      
        1 tacgtagggt ccaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgc
       61 aaagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgtaac tagcgggcta
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctagcactga cgctcatgca caaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003407
VERSION     KHUT01003407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           95 g           51 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggcggtttgt
       61 taagtcactg gtgaaagttt tcggcccaac cggaaaattg cctgtgatac tggcgaactt
      121 gagtgcgtga ggggtggggg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccgga gccgaagggg ccccactggt acgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003408             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003408
VERSION     KHUT01003408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttctt
       61 taagtctggt gtataagccc gtggctcaac cacgggacgc actggaaact ggggggctgg
      121 agtgcaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003409
VERSION     KHUT01003409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatacc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgggcgtctt
      121 gagccactta ggggcaagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gcttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003410
VERSION     KHUT01003410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcgggcgat
       61 taagcgtatt gtgaaatact agggcttaac cttagtgctg caatacagac tggttgtcta
      121 gagtagagga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttcctggt ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003411
VERSION     KHUT01003411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggctag
       61 taagtcggac gtgaaatccc tgggctcaac ccaggaactg catccgaaac tattagtctt
      121 gagtggtgga gagggaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cactaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003412
VERSION     KHUT01003412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           96 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtttcg
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggag cgccgaaaac tgcgggactg
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003413
VERSION     KHUT01003413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           91 g           46 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agggtctgta ggtggcctct
       61 tgagtctgct gttaaagatc agggcttaac cctgtaaagg cagtggaaac taagagggta
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccgac ggcgaaagca ctctgctggg ccgaaactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01003414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003414
VERSION     KHUT01003414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcggcg
       61 taagtcggat gtgaaatctc ggagcttaac tccgaaatgg cattggaaac tgcgttgctg
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003415
VERSION     KHUT01003415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgag
       61 tgtgttagat gtgaaatccc ggggctcaac cccggaactg cgtctaaaac tacttggcta
      121 gagtattgga gagggtatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gatacctgga caattactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01003416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003416
VERSION     KHUT01003416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtcgggg gtgaaatccc accgctcaac ggtggaagtg cccccgatac tgggagtctg
      121 gagggaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003417
VERSION     KHUT01003417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtctat
       61 cgcgtcggga gtgaaaagct ggggcttaac cccagtcctg ctttcgatac gggtggacta
      121 gaggtatgca ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003418
VERSION     KHUT01003418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           94 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgtgca ggcgggtgga
       61 taagtctgat gtgaaagccc ggggctcaac cccggaagtg cattggaaac tatccatctt
      121 gagtgctgga gaggaagggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cccttctgga cggcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003419
VERSION     KHUT01003419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           73 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggacatt
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaactg ccattgatac tatttgtctt
      121 gaattctctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactacg gagacattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003420
VERSION     KHUT01003420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           99 g           42 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcgggctgt
       61 caagtggtgc atgaaatcgc tcggcttaac cgggcgcggt tgtgccagac tgacagtcta
      121 gagtgcggga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc ccgacactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003421
VERSION     KHUT01003421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 taagtttgat gtgaaatccc ggggctcaac cctggaattg cattgaatac taccttgcta
      121 gagtaccgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cggtaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003422             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003422
VERSION     KHUT01003422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           82 g           55 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gacggtctga
       61 taagttattg gtggcaaacc gaggctcaac ctcgtggaag ctgataatac tgttagactt
      121 agagggcaga agaggttaac ggaattctct gtggagcggt aaaatgcgta gatatagaga
      181 agaacaccaa aaggcgaagg cagttaacta ttctgttcct gacgttgagg cacgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01003423             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003423
VERSION     KHUT01003423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           86 g           59 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtctga
       61 taagtcagtt gtgaaattcc tcggcttaac cgagtggact gcgattgata ctgtgagact
      121 tgaatcaggt tgaggtaggc ggaatgtgac atgtagcggt gaaatgctta gatatgtcat
      181 agaacaccga ttgcgaaggc agcttgctag acctggattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003424
VERSION     KHUT01003424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggaccag
       61 taagcgtcct gtgaaatccc atggctcaac catggaattg cagggcgaac tgctggtctt
      121 gaggaagcta gaggctggca gaacgattgg tggagcggtg gaatgcgtag atatcaatcg
      181 gaatgccgaa agtgaagaca gccagctggg gctttcctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01003425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003425
VERSION     KHUT01003425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           90 g           49 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggtttta
       61 caagtcagat gtgaaatatc cgggcttaac ccggaggggt catttgaaac tgtgagacta
      121 gagtacagca gaggatagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctggg ctgaaactga cgctgaggcg cgaaggctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003426
VERSION     KHUT01003426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           96 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggtgtcg
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tgcgagactc
      121 gagtgcggga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttcctgga ccgttactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003427
VERSION     KHUT01003427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctcaa
       61 caagtcaggt gtgaaagccc ccggctcaac cggggaactg cgcttgaaac tgttaggctc
      121 gagtcagcta gaggtacacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgtactggg gctggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003428
VERSION     KHUT01003428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           85 g           51 t
ORIGIN      
        1 gacagaggtc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgcttatctt
      121 gggtcatcta ggggcgagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gctcgctggg gatgtaccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003429
VERSION     KHUT01003429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           76 g           61 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtg gtgaaagccc caagcttaac ttgggaactg ccattgatac tgatgagctt
      121 gagtagattt gaggcgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgtaggca gctcgctaaa ctcttactga cgctgaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01003430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003430
VERSION     KHUT01003430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           84 g           49 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggctgtttgg
       61 caagttggaa gtgaaagccc ggggctcaac cccggaactg ctttcaaaac tgccagactc
      121 gaattcggga gaggatagcg gaatttccag tgtagtagtg aaatacgtag atattggaag
      181 gaacaccagt ggcgtaagcg gctatctgga ccgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003431
VERSION     KHUT01003431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcatgg
       61 caagtggaag gtgaaatccc tcggctcaac tgaggaactg cctaccaaac tgctttgctt
      121 gagtccggga gaggtaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gcttactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003432
VERSION     KHUT01003432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggctcg
       61 caagtcaggg gtgaaatcct ccggctcaac cggagaacag cctttgatac tgcgagcctc
      121 gaggaaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003433
VERSION     KHUT01003433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           84 g           55 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcgggaaag
       61 taagttagag gtgaaatctc acagctcaac tgtgaaactg cctttgatac tgcttttctt
      121 gagttaggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc cctaaactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003434
VERSION     KHUT01003434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           97 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggccctt
       61 caagtcgggc gtgaaagctc cgggcttaac ccggaaattg cgttcgatac tgtcgggcta
      121 gagtgtggaa ggggctaccg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003435
VERSION     KHUT01003435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           99 g           43 t
ORIGIN      
        1 tacgggggga gcgagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcggg
       61 taagtgggat gtgaaagccc cgggctcacc ccgggatcgt catctcaaac tgtccggctt
      121 gagtacagga gagggtgggg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg cccacctggc ctgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003436             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003436
VERSION     KHUT01003436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          103 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgctg
       61 caagtcggct tcgtgaaagc ccggggctca accccggagg tgcggccgag actgcggtgc
      121 tcgagtccgg aagaggaggg tggaataccc agtgtagagg tgaaattcgt agatattggg
      181 tggaacaccg gtggcgaaag cggctctctg gtccggcact gacgctgagg cgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01003437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003437
VERSION     KHUT01003437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           42 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggaagg
       61 taagtctgga gtgaaattcc ggggctcaac tccggagctg ctttggatac tgtctttcta
      121 gggaccggta gaggccggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccggctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003438
VERSION     KHUT01003438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggcgcgt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaatgg catccgatac tggcgggcta
      121 gagttcgata gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gcgacctgga tcagaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003439
VERSION     KHUT01003439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           85 g           48 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcagaatca ctgggcttaa agcgcgtgta ggtggacggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgcctgtctt
      121 gaggcactca ggggcagtcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaaa ggtgaaaaca gactgctggg agtgttctga cactgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003440
VERSION     KHUT01003440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          100 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaaatt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggagtgc ggctgcgaac tgggtttctt
      121 gaggacgata gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccga ggcgaaggcg gctctctggg tcgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003441
VERSION     KHUT01003441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           84 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggttcgg
       61 aaagtcagat gtgaaatccc acagctcaac tgtggaactg catttgaaac ttccagacta
      121 gagtatgaga ggggtaaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg ctttactggc tcattactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003442
VERSION     KHUT01003442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatca ctgggcgtaa agcgcgcgta ggcggcctta
       61 taagtcggat gtgaaatccc tcggctcaac cggggaactg cattcgatac tgtttggctg
      121 gagtgcagga gagggtggcg gaattcccgg tgtaggagtg aaatccgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003443
VERSION     KHUT01003443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           97 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggctcgc
       61 taagccagac gtgaaatccc tcagcttaac tggggaactg cgtctgggac tggagagctg
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003444
VERSION     KHUT01003444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcagtt gtgaaatctc tgggcttaac tcagagcggt caactgatac tgtcgtgcta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003445
VERSION     KHUT01003445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggaccat
       61 caagtcggat gtgaaatccc tcggcttaac cgaggacgtg cattcgaaac tgatggcctt
      121 gagtacgaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003446
VERSION     KHUT01003446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           89 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacaag
       61 taagtcgggc gtgaaatccc taggctcaac ctaggaactg cactcgaaac tgcatgtctt
      121 gagtaccgga gaggaaagcg gaatacctgg tgtagaggtg aaattcgtag atatcaggtg
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003447
VERSION     KHUT01003447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggtttgg
       61 taggtccgct gtgaaaactc gaggctcaac ctcgagacgc cggtggaaac catcaggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggctaaggcg gctctctagt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003448
VERSION     KHUT01003448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgt
       61 caagtcactt gtgaaatctc cgggcttaac tcggaacggt caagtgatac tgtcatgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctcaagcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003449
VERSION     KHUT01003449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggctaag
       61 taagtcgggg gtgaaaactc gaggctcaac ctcgagactg cctccgatac tgcgtagctt
      121 gagtatccta gagggtagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg actacctggg ggaaaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003450
VERSION     KHUT01003450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           80 g           61 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggagcgta ggtggcattc
       61 taagtctgat gtgaaagctc tgagcttaac tcagaaatgg cattagatac tagtttgctt
      121 gaggattgga ggggagattg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg aatctctgga caattcctga cactgatgct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003451
VERSION     KHUT01003451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           84 g           58 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgtgt
       61 taagtgtgat gttaaatcgt gtggctcaac catatcgatg tgtcgcatac tggcatgcta
      121 gagggaagca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg cttcttctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003452
VERSION     KHUT01003452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 taagtcagat gtgaaagccc ggggctcaac ctcggaattg catccgatac tgctatgctt
      121 gagtactgga gaggtgacta gaattctagg tgtagcagtg aaatgcgtag atatctagag
      181 gaataccaaa ggcgtaggca ggtcactgga cagttactga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003453
VERSION     KHUT01003453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           84 g           60 t
ORIGIN      
        1 tacgtagggg gttagcgttg tccggattta ctgggtgtaa agggcgcgta ggcgggtttg
       61 taagtcagtg gtgaaatcct gcagcttaac tgcagaactg cctttgatac tgcaaatctt
      121 gagttcagaa gagagagatg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt ggcgaaggcg atctcttggt ctgacactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003454
VERSION     KHUT01003454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           97 g           46 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggtgac
       61 caagtggtgt atgaaagggt caggcttaac ctggcgaggg tatgccagac tggtcgtcta
      121 gaacgtgaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaagcg gcgtcctggc tcactgttga cgctcagaca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003455
VERSION     KHUT01003455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatca ctgggcgtaa agggtgcgta ggcggtttgg
       61 caagtcagat gtgaaatcct acagcttaac tgtagaactg catttgaaac tgctaaacta
      121 gaatactgaa ggggcagatg gaattagtgg tgtaggggta aaatccgtag atatcactag
      181 gaataccgaa agcgaaagcg atctgctggg aagttattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003456
VERSION     KHUT01003456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           97 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtcaggg gtgaaagccc ccagctcaac tggggaagtg cctttgatac tggtggtctc
      121 gagggggata ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgtgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003457
VERSION     KHUT01003457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           62 c          111 g           36 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctggt
       61 cttgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggccagctt
      121 gaggacggca ggggcaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcctgctggg ccgatcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003458
VERSION     KHUT01003458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctctt
       61 caagtcagat gtgaaagccc cgggctcaac ctgggaactg catttgagac tgaagaactc
      121 gagttcggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003459
VERSION     KHUT01003459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctatg
       61 caagtcggat gtgaaagccc tgggcttaac ctaggaagtg cactcgaaac tgcatagctc
      121 gagtcccgga gaggatggtg gaattctgag tgtagaggtg aaattcgtag atattcagag
      181 gaacaccggt ggcgaaggcg gccatctgga cgggtactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003460
VERSION     KHUT01003460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           93 g           44 t
ORIGIN      
        1 aacgtaggaa gcaagcgtta tccggattta ctgggcgtaa agggcaggca ggcggtcggt
       61 taagtcacac ttgaaagctc tcggctcaac tgagagaggc ggtgggaaac tggcggacta
      121 gaggatggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccattactga cgctcaactg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01003461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003461
VERSION     KHUT01003461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           60 c          109 g           38 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtccgt
       61 caagcatggc gtgaaagccc ccggctcaac cggggagggt cgtcgtggac tggcggactt
      121 gagggcggta ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003462
VERSION     KHUT01003462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcttgt
       61 caggtctgat gtgaaacctt ggggcttaac tccaagcgtg catcggaaac cggctggcta
      121 gagtctggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagt agcgaaggcg gctctctgga acagtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003463
VERSION     KHUT01003463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtctatg gtgaaatcca acagcttaac tgttgaactg ccacggatac tgattgtctc
      121 gaggaaggta ggggcgtgcg gaacaaatgg tggagcggtg aaatgcattg atatcatttg
      181 gaactccggt ggcgaaggcg gcacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003464
VERSION     KHUT01003464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           86 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggtctgt
       61 taagtgagat gtgaaagcgt gtggctcaac catatcgttg tgtctcatac tggcagacta
      121 gaggacggca gaggcaagtg gaattagcag tgtagcagtg aaatgcgtag agattgctag
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003465
VERSION     KHUT01003465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggattta ttgggcgtaa agggcgcgta ggcggtcttg
       61 taagtcagat gtgaaagccc ggggcttaac cccggaaggg catttgatac tgcgagactt
      121 gagtatggga gggggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg acttcctgga ccaatactga cgctgatgcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003466
VERSION     KHUT01003466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           92 g           49 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggaagga
       61 taagtctact ctgaaagctc ctggctaaac tgggagaggc gagtggagac tgtttttcta
      121 gagtgaggaa gaggaaagtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaagcg actttctggt ccttaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003467
VERSION     KHUT01003467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           89 g           42 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggcttaa
       61 aaagtctcct gtcaaagatc agggcttaac cctgggccgg caggagaaac tcttaggcta
      121 gagtttggta ggggcagagg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccaaa ggcgaaagca ctctgctggg ccacaactga cactgagaga cgaaagcgag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01003468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003468
VERSION     KHUT01003468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           88 g           46 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 caagttcgat gtgaaagccc tcggctcaac caaggaagtg catcgaaaac tgcagagctt
      121 gagtacgaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003469
VERSION     KHUT01003469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           94 g           41 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggtcacc
       61 taagtcgaat gtgaaagccc tcggctcaac cggggaagtg catccgaaac tgggcgactg
      121 gagtaccaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggc ggcgaaggcg gcgacctggt tggatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003470             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003470
VERSION     KHUT01003470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           95 g           47 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcgaat gtgaaatctc cacgctcaac gtggaggggt cattcgatac tgccgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003471
VERSION     KHUT01003471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           81 g           53 t
ORIGIN      
        1 tacgaaggga gcaagcgtta ttcggaatca ctgggcgtaa agggcgtgta ggcggttaat
       61 taagtcagtt gtaaaattcc tcagcctaac tgaggcatcg caattgaaac tgattggcta
      121 gagtgcagaa gagagaagtg gaattatcgg agtagcggta aaatgcgtag atctcgatag
      181 gaacaccgat ggcgaaggca gcttcttggt ctgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003472
VERSION     KHUT01003472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcatgta ggcgggtcgg
       61 caagtcagat gtgaaatccc cgggctcaac ccgggaactg catctgaaac tgctggtctt
      121 gagtactgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cagtaactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003473             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003473
VERSION     KHUT01003473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           48 c           99 g           54 t
ORIGIN      
        1 tacggaggtg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggccct
       61 taagtgaggg gtgaaaggct gaggctcaac ctcatgtttg tcctctcata ctgtgggtct
      121 tgagtgaggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg acctttactg acgctgaggc gcgaaagctg
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003474
VERSION     KHUT01003474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           80 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cccttgatac tgatgggctt
      121 gaatgtacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactatg gtatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003475
VERSION     KHUT01003475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          100 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgcg
       61 caagtcggat gtgaaaaccc ggggctcaac cccgggcgtg cattcgatac tgcgcggctc
      121 gagacaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgttctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003476
VERSION     KHUT01003476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggccgac
       61 caagtcgaat gtgaaagccc ccggctcaac tggggagagt cattcgatac tgatcggctt
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003477
VERSION     KHUT01003477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          100 g           45 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgt
       61 tttgtcaggc gtgaaagccc tgggctcaac ctgggaactg cgcttgggac ggatgggcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003478
VERSION     KHUT01003478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           86 g           46 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttct
       61 tcagtctact gtcaaagatc agggcttaac cctgggcagg cagtcgaaac taagaagctt
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaac agcgaaagca ctctgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01003479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003479
VERSION     KHUT01003479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c           98 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggtcgcg
       61 ggcgtcggtg ctgaaagtcc accgcttaac ggtggatcgc gtgccgatac gccgcgactg
      121 gagccatgta gaggtccgtg gaattgccgg tgtagtggtg atatgcgtag atatcggcag
      181 gaacacccaa ggggaagcca acggactggg catgggctga cgctcatgtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01003480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003480
VERSION     KHUT01003480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggtggtgcgg
       61 taagttggga gtgaaatctc tgggcttaac tcaggggctg ctttcaaaac tgccgtgctc
      121 gagtgcgaga gaggtcagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctgactgga tcgcaactga cactgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003481
VERSION     KHUT01003481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           99 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggccgcg
       61 tgagtcggct gtgaaagctc ctggcttaac taggagaggc cagtcgatac tgcggggctt
      121 gaggttggga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003482
VERSION     KHUT01003482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccttt
       61 caagtcaggg gtgaaagccc ggagctcaac tccggaattg cctttgaaac tgtcaggctc
      121 gagtacggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc ccgtttctga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003483
VERSION     KHUT01003483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          104 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcagtg
       61 caagtacggc gtgaaatctc cctgctcaac ggggagggtg cgctgtagac tgcacagcta
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgatcctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003484             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003484
VERSION     KHUT01003484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           57 c           91 g           42 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagcgt
       61 taagtgtccg tgtgaaatcc ctcggctcaa ccggggaact gccgggcata ctggcgctct
      121 tgagcggtcc aggggtcatc ggaacaaacg gtggagcggt gaaatgcgta gatatcgttt
      181 ggaacgccga cggtgaaaac agatgactgg ggacctgctg acgctgagac acgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003485             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003485
VERSION     KHUT01003485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c          106 g           33 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggtgcg
       61 gaagtcgggg gtgaaaggcc ggggctcaac cccggatcgt tctctcgata ctccgtgcct
      121 agggaccggt agaggccggt ggaattcccg gagtagcggt ggaatgcgta gagatcggga
      181 agaacaccag aggcgaaggc ggccggctgg gccggatccg acgctcaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003486
VERSION     KHUT01003486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 taagacagat gtgaaatccc cgggctcaac ctgggaactg cgtttgtgac tggcgggctg
      121 gagtacggca gaggggggtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatcgat ggcgaaggca gccccctggg ccgctactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003487             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003487
VERSION     KHUT01003487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           93 g           49 t
ORIGIN      
        1 cacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 ttagtcgggt gtgaaaactc tgggctcaac tcagagacgc cacccgatac tgctgtgact
      121 tgagtccggt aggggagcag ggaattccag gtgtagcggt gaaatgcgca gatatctgga
      181 ggaacaccgg tggcgaaggc gctgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003488
VERSION     KHUT01003488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           48 t
ORIGIN      
        1 aacgtaggtt ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggtttag
       61 caagtcgggc atgaaatctc tcggctcaac cgggaggggc tgtccgaaac tgctagactt
      121 gagggcaata gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaagcg gccacctgga ttgcacctga cgctcaaaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003489
VERSION     KHUT01003489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggccaga
       61 ttagtccggt gtgaaagtca aaggctcaac ctttgaatgc cgctggaaac tgtctggcta
      121 gagtccggga ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctgga acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003490
VERSION     KHUT01003490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtctg
       61 aaagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tacaggtctt
      121 gaattttctg gaggatagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctatctaca gggatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003491
VERSION     KHUT01003491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacggaccgg acaaacgtta ttcggaatca ctgggcttaa agagtacgta ggcggctcag
       61 aaagttggat gtgaaatccc tcggctcaac cgaggaattg cgttcaaaac tactgagctt
      121 gaggaaggta ggggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaggcg gctcactggg ccttttctga cgctgaggta cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003492
VERSION     KHUT01003492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          100 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgtg
       61 caagtcggac gtgaaatacc acggctcaac cgtggggagc cgttcgatac tgctcagctg
      121 gaggcaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01003493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003493
VERSION     KHUT01003493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           85 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggaagat
       61 taagtcagag gtgaaagact gcagctcaac tgtagcagtg cctttgatac tgattttctt
      121 gagtacagat gaggctggcg gaatgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gccggctaaa ctgttactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01003494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003494
VERSION     KHUT01003494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          103 g           37 t
ORIGIN      
        1 gacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggcttct
       61 caagcccggg gtgaaaggcc ccggcccaac cggggacggt cttcggggac tgagaggctc
      121 gggcgagcta ggggtccgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acggactggg gctttgccga cgctcaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003495             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003495
VERSION     KHUT01003495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           84 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agagggagca ggcggctata
       61 aaggtctgtg gtgaaagact gaagctcaac ttcagtaagc catggaaacc aaatagctag
      121 agtgcattag aggatcgtgg aattccatgt gtagcggtga aatgcgtaga tatatggagg
      181 aacaccagtg gcgaaggcga cgatctggga tgtaacagac gctgagtccc gaaagcgtgg
      241 ggagcaaata gg
//
LOCUS       KHUT01003496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003496
VERSION     KHUT01003496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatccc cgagctcaac ttgggaactg ccgttgatac tatctgtctt
      121 gaatatcgtg gaggtgagcg gaataagtca tgtagcggtg aaatgcttag atatgactta
      181 gaacaccgat tgcgaaggca gctcactact cgatcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003497
VERSION     KHUT01003497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcatat
       61 ccagttaggc gtgaaattcc tgggctcaac ctggggactg cgcttaatac aggtaggcta
      121 gagtgtgaaa gagggtcgtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg gcgacctggt tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003498
VERSION     KHUT01003498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc ccggcttaac cggggaattg cgcccaaaac tactgtgcta
      121 gaggaataca gaggtaagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tattttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003499
VERSION     KHUT01003499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           75 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggacttt
       61 taagtcagag gtgaaatctc cgggctcaac ccggaaactg cccctgatac tattggtctt
      121 gaatatcgtt gaggtaggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccaat tgcgaaggca gcttactaaa cgattattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003500
VERSION     KHUT01003500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          106 g           37 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggattcg
       61 cgcatcatcg gtgaaagccc cccgctcaac gggggagggt ccggtgagat ggcgagtctg
      121 gaggcaggga gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctcgctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003501
VERSION     KHUT01003501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccaga
       61 caggtccgtt gtgaaaactg gaggctcaac ctccagctgt cgatggaaac cgtttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003502
VERSION     KHUT01003502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 aacgtaggac gcgaacgtta tccggaatta ctgggcgtaa agcgcgcgca ggtgggtgaa
       61 caagtcatgc gtgaaagctc ccggctaaac tgggagaggt cggatgatac tgttcgtcta
      121 gagtatgtta gaggcgtgtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gcacgctggg acattactga cactgaggcg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003503
VERSION     KHUT01003503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctc
       61 taagtcccgt gtgaaagccc tcggcttaac cgaggaactg cacgagaaac tggagagctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003504
VERSION     KHUT01003504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           99 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg cctcggatac tggtcgcctc
      121 gagggagata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003505
VERSION     KHUT01003505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgtag
       61 caagtcgggc gtgaaagccc acggctcaac cgtggaagag cactcgaaac tgctgcgcta
      121 gagttctgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003506             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003506
VERSION     KHUT01003506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          100 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtcaat
       61 taagtcttac cgtgaaatgc cggggctcaa ccccgcgcac gtcgggggat actggttgac
      121 tggagacaag tagaggcgag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacacca gaggcgaagg cggctcgctg ggcttggtct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01003507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003507
VERSION     KHUT01003507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttatg
       61 tcagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tgcatggcta
      121 gagtttggta gaggctagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gctggctgga ccaaaactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003508
VERSION     KHUT01003508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggaagct
       61 taagtgtgat gttaaatcgt gtggctcaac catatcgatg cattgcatac tgggtttcta
      121 gaggacggca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccgcccctga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003509
VERSION     KHUT01003509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgt
       61 tcagtcagaa gtgaaagccc cgggctcaac ctgggaacgg cttttgatac tggcgggctt
      121 gagttccgga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga cggacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003510
VERSION     KHUT01003510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           88 g           45 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctctg
       61 caagtccgat gtgaaagccc acggctcaac cgtggaagtg catcggaaac tgcaaagctt
      121 gagtacaaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg ttgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003511
VERSION     KHUT01003511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcccga
       61 taagtcggat gtgaaatccc ggagctcaac tccggaactg cacccgatac tgtcgggctc
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003512             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003512
VERSION     KHUT01003512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           89 a           26 c           67 g           72 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agggcatgta ggtggtttgt
       61 tatatgtata ctatgaaatt gtaaagcata actttatatt gtagtataaa caactaaact
      121 tgagttaggt agaggatagt agaatttcta atgtaaaggt gaaatttgat gaaattagag
      181 agaataccaa tggcgtaggc aactatctgg gccttaactg acactgaggt gcgaaagtat
      241 ggggagcaaa tagg
//
LOCUS       KHUT01003513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003513
VERSION     KHUT01003513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           96 g           42 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatccc gcggctcaac cgcggaactg cctcagatac tgacggtctc
      121 gagggaggta ggggcgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaactccggt ggcgaaggcg gctcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003514
VERSION     KHUT01003514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgcg
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catcttagac tgcgtagctg
      121 gagtgcggta gagggtagag gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003515
VERSION     KHUT01003515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcgggtcgt
       61 taagttaggg gtgaaatccc aaggctcaac cttggaactg cctctaaaac tggcgacctg
      121 gagcagatga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actcgctggc atcttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003516
VERSION     KHUT01003516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          103 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggtttcg
       61 ggcgtcggtg ctgaaagtcc accgcttaac ggtgggaagc gtgccgatac gccgaaactg
      121 gaggctgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt ggtgaaaacg gccacctagc ccagacctga cgctgaggcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003517
VERSION     KHUT01003517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggattcg
       61 taagtcagtg gtgaaatctc atagcttaac tatgaaactg ccattgatac tgcgagtctt
      121 gagtgagatt gaggtagctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggttaccaag tcttaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003518
VERSION     KHUT01003518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           86 g           45 t
ORIGIN      
        1 gacgaaggtg acaaccgtta ctcggaatca ctgggcataa agcgcgcgta ggtggcttgg
       61 taagtccatg gtgaaatccc tcggctcaac cgaggaactg ccgtggatac tgccgagctt
      121 gagaacggga ggggtaagtg gaactctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacgccggt ggcgaaagcg acttactgga ccgtttctga cactcaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003519
VERSION     KHUT01003519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           80 g           59 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtgggtttt
       61 taagtcagaa gtgaaagccc ggagctcaac tccggagttg cttttgatac tatttacctt
      121 gagtgtagga gagggcaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttgcctggc ctatcactga cactaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003520
VERSION     KHUT01003520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctctc
       61 cacgtcgggt gtgaaagctc agagctcaac tctgagaggc cattcgatac gggagggcta
      121 gaggaaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctttcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003521
VERSION     KHUT01003521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcggat gtgaaagccc acggctcaac cgtggaagtg cactcgaaac tgcagagctg
      121 gagtctcgga gaggaaggcg gaatactcgg tgtagaggtg aaattcgtag atatcgagtg
      181 gaacaccggc ggcgaaggcg gccttctgga cgatgactga cgctgaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003522
VERSION     KHUT01003522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgca ggcggattga
       61 taagttaggg gtgaaatccc gaggcttaac ctcggaactg cctttgatac tgtcagtctt
      121 gagaccgaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003523
VERSION     KHUT01003523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcccac
       61 taagtcagtt gtgaaagccc ccggctcaac cggggaatgg catctgatac tggtgggctc
      121 gaggaaggta ggggtgggcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcccactgga ccttctctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003524
VERSION     KHUT01003524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ctgggcataa agagcacgta ggcggctaag
       61 taagtcggat gtgaaatacc tcggctcaac cgaggaactg cgtccgatac tgcttggctt
      121 gaggtccgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cggaatctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003525
VERSION     KHUT01003525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggtgcgg
       61 taagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac taccacactc
      121 gagggagata gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003526
VERSION     KHUT01003526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctggg
       61 aaagtcggtg gtgaaagctc gcggctcaac tgcgagtcgg ccatcgatac ttctcggctc
      121 gagtcgacta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg gtcgaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003527
VERSION     KHUT01003527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agggtgtgta ggtggctatg
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg catctgatac tgcgtagctt
      121 gagtaccgaa gaggatagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctatctggt cgagtactga cactgagaca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01003528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003528
VERSION     KHUT01003528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           99 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtctgcg gtgaaaactc ggggcttaac ctcgggcgtg ccgtgggtac gggcaggcta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccataactga cgctgagaag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003529             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003529
VERSION     KHUT01003529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           91 g           47 t
ORIGIN      
        1 tacagggggt gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttttt
       61 gaagtccact gtcaaatccc agggctcaac cttggacagg cagtggagga ctcaaaggct
      121 agagtgcggt aggggtagag ggaattccta gtggagcagt gaaatgcaca gagattagga
      181 agaacaccag tggcgaaggc gctctactgg gccgagactg acactgagag acgacagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01003530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003530
VERSION     KHUT01003530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           98 g           47 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtccta
       61 tgtgtctact gtgaaagcac acggctcaac cgtgtcgggt cggtagaaac tgtaggactt
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cactgaggcg cgacagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003531
VERSION     KHUT01003531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggtcgga
       61 taagtgggat gtgaaacccc tgggcttaac ctaggacgtg cataccagac tgtcgggcta
      121 gagtatggga gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gcctcctggc ctaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003532
VERSION     KHUT01003532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgt
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaactg ccgtggatac tggcggtctc
      121 gaggatggta ggggcgtgcg gaacagttgg tggagcggtg aaatgcgttg atatcaactg
      181 gaactccggt ggcgaaggcg gcacgctgga ccatgtctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003533
VERSION     KHUT01003533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           59 c          111 g           37 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggcttcg
       61 cgcgccccat gtgaaagccc ccggcttaac cggggagagt cgtgggggac ggcgaggctt
      121 gagggcggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccgactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003534
VERSION     KHUT01003534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           92 g           45 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcc
       61 caagtcggtg gtgaaatctt tcggcccaac cggaaaacag ccgccgatac tgggagtctt
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003535
VERSION     KHUT01003535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcccgg
       61 aaagtcggat gtgaaagccc tgggcttaac ctaggaagtg cattcgaaac tgccgggctt
      121 gagttctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003536
VERSION     KHUT01003536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggaccgc
       61 taagtcggat gtgaaatccc tcagcttaac tgaggaagtg cactcgaaac tgacggtctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003537
VERSION     KHUT01003537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgctc
       61 taagttagat gtgaaaactc tgggctcaac ccagagcctg catctaaaac tggagtgcta
      121 gagttctgga gggggtagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagaaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003538             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003538
VERSION     KHUT01003538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c          101 g           44 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggattca ttgggcgtaa agggctcgta ggcggacagg
       61 taagtctggc gtgaaatctc caccactcaa cggggagagg tcgtcggaaa ctgcttgtct
      121 ggagtacggt agaggaccat ggaattccag gtgtagcggt ggaatgcgta gagatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgtgactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003539
VERSION     KHUT01003539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           84 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 taagtcagat gtgaaagccc agggctcaac cctggaattg catccgatac taccatgcta
      121 gagtactgaa gaggtgacta gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaataccaaa ggcgaaggca ggtcactggg cagttactga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003540
VERSION     KHUT01003540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c          106 g           31 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggtggcc
       61 gacgtgccgt gtgacaagcg ggggctcaac ccccgagggc cacggtaaac ggggtcactg
      121 gaggaaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc ccttacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01003541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003541
VERSION     KHUT01003541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtttc
       61 caagtcagat gtgaaatccc cgggctcaac ccgggaactg catttgaaac tgggagtctt
      121 gagtatcgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003542             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003542
VERSION     KHUT01003542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccggagaggc cactcgatac tgctgtgact
      121 tgagtctggt aggggagcac ggaattcctg gtgtagcggt gaaatgcaca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003543             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003543
VERSION     KHUT01003543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           51 t
ORIGIN      
        1 cacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcaggt gtgaaaactc tgggctcaac tcagagacgc cacttgatac tgtcgtgact
      121 tgaatctggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtattg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003544
VERSION     KHUT01003544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtctggg gtgaaagccc gttgctcaac aacggaactg ccctggaaac tggagagctt
      121 gagtacagac gagggcggcg gaatggaggg tgtagcggtg aaatgcatag ataccctcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003545
VERSION     KHUT01003545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          102 g           44 t
ORIGIN      
        1 tacgtaggag acaagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggtggtaggc
       61 caagtctgct gtgaaatcgc ccggctcaac tgggcgcggt cggtggaaac tggtcaactg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003546
VERSION     KHUT01003546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           90 g           46 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggaacta
       61 taagtctatt ctgaaagctc ctggctaaac tgggagaggc gaatggagac tgtagatcta
      121 gagtagggaa gaggaaagtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaagcg actttctggt cccaaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003547
VERSION     KHUT01003547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           48 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggttttg
       61 tgtgtctacc gtgaaagcac acggctcaac cgtgttacgt cggtagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccccg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagcactga cactgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003548             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003548
VERSION     KHUT01003548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           88 g           50 t
ORIGIN      
        1 cacgtaggct ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttag
       61 taagtcgggt gtgaaaactc tgggcttaac ccagagacgc cacccgatac tgctatgact
      121 tgagtacggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc gccactctgg gccgtaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003549
VERSION     KHUT01003549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 gacgaaggtg gctagcgttg ctcggaatta ctgggcataa agcgcgcgta ggtggttttg
       61 taagcgaggg atgaaatccc atggctcacc catggaactg tctctcggac tgcaaagctt
      121 gagggcggga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg actctctggc ccgttcctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003550
VERSION     KHUT01003550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 gacagaggtt gcaagcgttg ttcggaatta ctgggcataa agcgcacgta ggcggtccac
       61 taagtcatgt gtgaaatccc ccggctcaac cggggaatgg cgcctgatac tggtggactc
      121 gagatcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg actcactgga ccgacactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003551
VERSION     KHUT01003551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 cacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagttgatc gtgaaagctc ccggctcaac tgggagaggt cgttcaatac taccgaacta
      121 gagagtagaa gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctggt ctatttctga cgctcagacg cgacagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01003552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003552
VERSION     KHUT01003552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c          101 g           36 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggtcatc
       61 gacgtgccgt gtgacagcca tcggctcaac cgatggaggc cagggtacac gagatgactg
      121 gaggcagaga gagggatgtg gaatacctgg tggagcggtg aaatgcgtag agatcaggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc tctgtcctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01003553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003553
VERSION     KHUT01003553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           74 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtcagtg gtgaaatctt caagcttaac ttggaaactg ccattgatac tatagatctt
      121 gaattatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactacg gattgattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003554
VERSION     KHUT01003554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           74 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc cgagcttaac ttggaaactg ccattgatac tatcaatctt
      121 gaatactgtg gaggtaagcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat agcgaaggca gcttactacg cagttattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003555
VERSION     KHUT01003555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttgtt
       61 taagtccgtt gtgaaagccc tgggctcaac ctgggaattg cagtggatac tgggcaacta
      121 gagtgtggta gagggtggcg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccacctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003556
VERSION     KHUT01003556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           51 c          104 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggct gtgaaatccc gtggctcaac tgtgggcggg cagctgatac gggcaggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003557             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003557
VERSION     KHUT01003557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c          100 g           42 t
ORIGIN      
        1 gacggaggag gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggatcgg
       61 catgtctggc gtgaaatctc caccgcttaa cggggagagg tcgctggaaa ctaccgatct
      121 ggagttcggc agagggccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggcctgg gccgaaactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003558
VERSION     KHUT01003558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           49 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggctttc
       61 caagtcactt gtgaaatctc cgggcttaac tcggaacggt caagtgatac tgtgaggcta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgatgcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003559
VERSION     KHUT01003559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          100 g           42 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggatgg
       61 taagtcggtc gtgaaagcct tcggctcaac cgaagaacgt cggccgaaac tgccattctt
      121 gagggatgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg catctcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003560
VERSION     KHUT01003560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgattgag
       61 gcagtcttgt gtgaaagccc atagcttaac tatggaagtg cacaggatac ttctcaattt
      121 gagtgcagga gaggaaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccttctgga ctgctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003561
VERSION     KHUT01003561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          103 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtccgc
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccatgcgaac tggtggactg
      121 gagcctgtca gaggcaagcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcttgctggg acagtgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003562
VERSION     KHUT01003562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           90 g           46 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcccgg
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcccaaaac tgtcgggctt
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggg ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003563
VERSION     KHUT01003563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcgggtcgt
       61 taagttgggg gtgaaagccc ggagctcaac tccggaactg ccttcaaaac tggcgacctg
      121 gagcaagata gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actcgctgga tctttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003564
VERSION     KHUT01003564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           83 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggccgcgcgg
       61 gcagtcaaat gtgaaatcct ggggctcaac ctcagaattg cgcttgatac ttccgcgcta
      121 gaactttgga gagggatctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacactcag ggcgtaggca agatcctgga caaatgttga cgctcatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003565
VERSION     KHUT01003565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggatagg
       61 taagtgggat gtgaaatccc tcggctcacc cgaggacctg catcccaaac tgccagtctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003566             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003566
VERSION     KHUT01003566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           78 g           57 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttattg gtcgaaatcg agctcaactt gatggaggct agtaatactg ttctactaga
      121 gggcagaaga ggttagcgga acttacacaa gtaggggtga aatccaatga tctgtgaggg
      181 accaccaaaa aaggcgaagg cagctaacta ttctgttcct gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01003567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003567
VERSION     KHUT01003567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           93 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggcagat
       61 taagtgagtt gtgaaatccc tgggcttaac ctaggaactg cgacttatac tggtttgcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctatctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003568
VERSION     KHUT01003568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           80 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggaacaa
       61 taagtcagtg gtgaaatctc tcggctcaac cgagaaattg ccattgatac tgttgttctt
      121 gaattcggtc gaagtgggcg gaatgtgaca tgtagcggtg aaatgcttag atatgtcaca
      181 gaacaccgat agcgaaggca gctcactagg cctggattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003569
VERSION     KHUT01003569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcttaa
       61 taagtcagag gtgaaaggtg gcagcttaac tgtcaaattg cctttgaaac tgttgagctt
      121 gaatcaagat gaggttggcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcacg
      181 gaacaccgaa tgcgaaggca gctgactgga cttagattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003570
VERSION     KHUT01003570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           37 c           83 g           63 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggattaa
       61 taagtctggg gtgaaagtct gtcgcttaac gatagaattg ccatggatac tgttgatctt
      121 gaatagagtt gaggatagcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccaat tgcgaaggca gctatctggg cttttattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003571             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003571
VERSION     KHUT01003571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           79 g           60 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtgtga
       61 taagttatca gtggcaaatc ggggctcaac ctcgtggaag ctgttaatac tgtcatgctt
      121 gagttcagga gaagttaacg gaactccatg tggagcggta aaatgtgacg atatatggaa
      181 gaacaccgat aaggcgaagg cagttaacta ttctgttact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01003572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003572
VERSION     KHUT01003572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggattgg
       61 tcagttaggg gtgaaatcct ggagctcaac tccagaactg cccttaatac tgccagtctc
      121 gagtccggaa gaggtaagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggcactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003573
VERSION     KHUT01003573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctcta
       61 taagtcagga gtgaaagccc caagcttaac ttgggaagtg cttttgatac tgcagagctt
      121 gaatgtggga gagggtagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctacctggc ccaacattga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003574
VERSION     KHUT01003574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggttgg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tatcaatctt
      121 gaatatcgtt gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactgag cggtcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003575
VERSION     KHUT01003575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacgaaggtc ccgagcgtta ttcggaatca ctgggcgtaa agggagcgta ggcggcgcgg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg catctgatac tgccgtgcta
      121 gagtaatgga ggggtagctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagag
      181 gaacactcgt ggcgaaagcg agctactgga catttactga cgctgaggct cgaaggccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01003576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003576
VERSION     KHUT01003576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccgg
       61 taagtccgct gtgaaagtca agggctcaac ccttgaaagc cggtggatac tgtcgggcta
      121 gagtgcggga gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctgga acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003577
VERSION     KHUT01003577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agcgtgcgta ggcggctttt
       61 taagtcggat gtgaaatccc tgagcttaac ttaggaattg cattcgatac tggaaagcta
      121 gagtatggga gaggatggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccgat ggcgaaggca gccatctggc ctaatactga cgctgaggta cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003578
VERSION     KHUT01003578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcttcg
       61 taagtcagag gtgaaatccc ggggctcaac cccggaactg cctttgaaac tgcagagctc
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003579
VERSION     KHUT01003579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcgagg
       61 taagtctggt gtgaaatcct ggggctcaac cccagaattg cactggatac tgctttgcta
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttcctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003580             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003580
VERSION     KHUT01003580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c          100 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgagca ggcggccctg
       61 taagtggtgt gtgaaagcgc tcaggctcaa ccgggcgagg acatgccaga ctgcggggct
      121 ggaggtaggt agaggcgagt ggaattccgg gtgtagtggt gaaatgcgta gagatccgga
      181 ggaacaccag tggcgaaggc gactcgctgg gcctaaactg acgctcagac gcgaaagcat
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003581             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003581
VERSION     KHUT01003581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggtctgt
       61 taagtcgttt gtgaaaatcc ggggctcaac cccgtgaaat gcgtacgata ctggcaggct
      121 ggagatcggt agaggctagc ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacacccg tggcgaaggc ggctagctgg gccgagtctg acactcatcc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003582
VERSION     KHUT01003582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggcattg
       61 caagtagggt gtgaaagccc tcggctcaac cgaggaattg cgctctaaac tgcagtgctt
      121 gaggaaccta gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg agttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003583             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003583
VERSION     KHUT01003583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           97 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggtcgaa
       61 aaagtctatg ggtcaaaggc cggggctcaa ccccgagtac gcccaggata ctgttcgact
      121 ggagacacgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003584
VERSION     KHUT01003584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagttcggt gtgaaagccc tcggctcaac cggggaagtg cgccggatac tgacagactt
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003585
VERSION     KHUT01003585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           49 c          103 g           53 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtctgcc gtgtaagcct tcggcttaac tgggggtttg cggtggatac gggttggctg
      121 gaggcaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttgcctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003586
VERSION     KHUT01003586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgctg
       61 caagtcaggc gtgaaattcc tgggctcaac ctggggactg cgcttgagac tgtggtgcta
      121 gaggtcagaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ctgaaactga cgctgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003587             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003587
VERSION     KHUT01003587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           51 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttaag
       61 taagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac tgctttgact
      121 tgagttcggt aggggtaagc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga cggcgaaggc agcttactgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003588
VERSION     KHUT01003588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttctg
       61 taagtcccgc gtgaaatccc ccggctcaac tggggaactg cgcgggaaac tgtggggcta
      121 gagttcagga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003589
VERSION     KHUT01003589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggactgc
       61 taagtcggac gtgaaatccc tcagcttaac tgaggaagtg cattcgaaac tggcagtctt
      121 gagtacagga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003590
VERSION     KHUT01003590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           88 g           47 t
ORIGIN      
        1 gacgagcctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaattg cgctcaaaac taccgtgctc
      121 gagggagata gaggagcgcg gaacttaggg tggagcggtg aaatgcgttg atatcctagg
      181 gaacaccggt ggcgaaagcg gcgctctgga tctcatctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003591             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003591
VERSION     KHUT01003591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           99 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatga ctgggcttaa agagcatgta ggcggatcgg
       61 aacgtccgtg gctgaaagcc cccggctcaa ccggggaagt ggcgcggata cgaccggtct
      121 cgaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accctttctg acgctgagat gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003592
VERSION     KHUT01003592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           99 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccatc
       61 taagtcggac gtgaaatccc tcggcttaac cggggaactg cgtccgatac tggagggctt
      121 gagtgtggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccatgactga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003593
VERSION     KHUT01003593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc cgagcttaac ttggaaactg ccgttgatac tatctgtctt
      121 gaatgctgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctaactaca cagttattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003594
VERSION     KHUT01003594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           93 g           44 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tcccgcgctt
      121 gagaactgga ggggacatcg gaattcacgg tggagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagt ggcgaaggcg gatgtctgga cagtatctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003595
VERSION     KHUT01003595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           88 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggcagtg
       61 taagtctgct gtcaaagaat gaggcttaac cttatcaagg cagtggaaac tacacagcta
      121 gagtacggtc ggggtagaag gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaagcg ttctgctaga cctgtactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01003596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003596
VERSION     KHUT01003596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           98 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggccggg
       61 tacgtggggt gtgaaaacgc tcggcttaac tgagcgaggt cacctcatac ggcacggctg
      121 gagctctgca gaggtccatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg cagcagctga cgctgatgtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003597
VERSION     KHUT01003597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           52 c          104 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgt
       61 cgcgtctgtc gtgaaagccc gcggctcaac tgcgggtctg cggtggatac gggcggactg
      121 gagtccgaca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg tcggtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003598
VERSION     KHUT01003598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggttcgg
       61 caagtctcta gtgaaatctc tgggctcaac tcagagtctg ctggggaaac tgccgggctt
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctctctgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003599             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003599
VERSION     KHUT01003599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccatt
       61 caagtcgggg gtgaaatccc agagctcaac tctggaattg ccttcgagac tgattggctt
      121 cgagtacggg agaggtgagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggctcactgg cccgttactg acgctcaggc gcgacagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01003600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003600
VERSION     KHUT01003600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggatga
       61 taagtcggaa gtgaaatccc tgggctcaac ccaggaactg ctttcgaaac tgtcgtcctc
      121 gagtaccgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003601
VERSION     KHUT01003601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           83 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggttgga
       61 taagttagat gtgaaatccc cgggcttaac ctgggcactg cgtttaagac tgtttaacta
      121 gagtactgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003602
VERSION     KHUT01003602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggctgtttgt
       61 taagtctggt gttaaagact gaggcccaac ctcggaaatg catcggatac tggcagactt
      121 gagtacggta gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaggcg actcgctggg ccgcaactga cgctgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01003603             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003603
VERSION     KHUT01003603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           43 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcagc
       61 gcaagtcagt tgtgaaatct cggggcttaa ccccgagcgg tcaactgata ctgcgcgact
      121 agagtgcgga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003604
VERSION     KHUT01003604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcaat
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cactcgatac tgcttgactc
      121 gaaggcggga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acttcctggc ccgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003605
VERSION     KHUT01003605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagag gtgaaatccc cgggcttaac ctgggaatgg ccacttatac tgtataactg
      121 gagtgcaata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ttgacactga cactgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003606
VERSION     KHUT01003606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           78 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatcct ggggcttaac cccagaactg ccgttgatac tatctgtctt
      121 gaataccgtg gagatgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgttaca cgaatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003607
VERSION     KHUT01003607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 cgagtcggaa gtgaaatctc agggcttaac cctgaaaccg cttctgatac tggcggactt
      121 gaggcaggta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003608
VERSION     KHUT01003608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 aacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggtctgta ggcggtgcgg
       61 caagtcggaa gtgaaatctc agggcttaac cctgaggctg ctcccgaaac tgctgtgctc
      121 gagtgcgaga gaggcgagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacatccga ggcgaaagcg gctcgctgga tcgcaactga cgctgagaga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003609
VERSION     KHUT01003609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctggt gttaaagccc ggagctcaac tccggcatgg cattggaaac tgggtggcta
      121 gagtgtggta gaggtcacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtgactggg ccacaactga cgctgaggaa cgaaagccag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01003610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003610
VERSION     KHUT01003610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           97 g           42 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggactta ctgggcgtaa agcgcgtgca ggcggacgga
       61 gatgtgccgt gtgaaagcgc cgggttcaac tcggcgaggc cacggtagac actgcgtctt
      121 gaggctccga gaggatggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccgat ggcgaaggca gccatctggc ggagacctga cgctgagacg cgacggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003611
VERSION     KHUT01003611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           83 g           56 t
ORIGIN      
        1 tacgtagggt acaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggggata
       61 taagtcaggt gtgaaatacc aaagctcaac tttggtgctg catttgaaac tgtatttctt
      121 gaggactgac ggagaaagcg gaattccacg tgtagcggtg aaatgcgtag atacgtggag
      181 gaacaccggt ggcgaaagcg gctttctagt ttttacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01003612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003612
VERSION     KHUT01003612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           90 g           53 t
ORIGIN      
        1 tacggagggt gccagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggagat
       61 taagtcgggg gtgaaagacg gttgctcaac aaccgcagtg ccttcgatac tggttttctt
      121 ggggggagtc gaggcggatg gaatgcaagg tgtagcggtg aaatgcatag ataccttgca
      181 gaactccgat tgcgaaggca gtccgctagg ctcttaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003613
VERSION     KHUT01003613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttatt
       61 taagacggtt gtgaaatccc tgggcttaac ctaggactgg caagcgagac tgaatgactg
      121 gagtacggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003614
VERSION     KHUT01003614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcggatgta ggtggctttg
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg catttgatac tgcgaagctt
      121 gaatgttgga gaggctacta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccgga ggcgaaggcg ggtagccagc caaacattga cactcagatc cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003615
VERSION     KHUT01003615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 taagtggaat gtgaaatgct ccggctcacc cggagcactg catcccaaac tgccgagctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003616
VERSION     KHUT01003616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatca ctgggcgtaa agcgcgcgta ggcggctaag
       61 taagttgggt gtgaaagccc tgggcttaac ccgggaactg cattcgatac tgcttggcta
      121 gagtatggta gagggaagcg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003617
VERSION     KHUT01003617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           62 c          110 g           33 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gaagtcggcg gtgaaagccc ggggctcaac cccgggtcgg ccgtcgagac tcccgggctc
      121 gagcacacta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg gtgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003618
VERSION     KHUT01003618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           94 g           53 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttgg
       61 taagtctttt gtgaaatctc tgggctcaac tcagagtctg caggagaaac tgccgggctg
      121 gagtgtggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003619
VERSION     KHUT01003619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c          102 g           43 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtaa agggagcgta ggcggtttgg
       61 taagccctct gggaaatctc ccggcttaac cgggaaaggt cagaggggac tgcctgacta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgttcctga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003620
VERSION     KHUT01003620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtttgc
       61 taagtcggtg gtgaaatact tcggctcaac cggagaattg ccgctgaaac tagcaggctg
      121 gagggaggta gaggtgcacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgcctgt ggcgaaagcg gtgcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003621
VERSION     KHUT01003621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtcagc
       61 caagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggctggcta
      121 gagtatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gctttctgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003622
VERSION     KHUT01003622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           93 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgca ggcggcttcg
       61 caagtcaggt gtgaaagccc cgggcttaac ctgggaattg cgcttgaaac tatggagctc
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg cctgcactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003623             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003623
VERSION     KHUT01003623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c           98 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggcctga
       61 ccaagtcgtg cgtgaaatct ctcggctcaa ctgggagcgg tcgtgcgata ctggtcggct
      121 agaggtcggt agggggaagt ggaattccgg gtgtagtggt ggaatgcgta gatatccgga
      181 ggaacaccag tggcgaaggc ggcttcctgg accgattctg acgctcagga gcgaaagcgt
      241 gggtagcgat ccgg
//
LOCUS       KHUT01003624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003624
VERSION     KHUT01003624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           77 g           64 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggttaga
       61 taagtcagtg gtgaaagact tcagcttaac tgaagcattg ccattgatac tgtttagcta
      121 gagtacagta gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgtaggca gcttactgga ctgtaactga cgctgaggca cgaaagtgcg
      241 ggtatcaaac agg
//
LOCUS       KHUT01003625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003625
VERSION     KHUT01003625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtccg
       61 caagtcgggg gtgaaatctt tcagctcaac tggaaaagtg cccccgatac tgcgggtctc
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003626
VERSION     KHUT01003626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagcgcgta ggcggtttgg
       61 tcagacagtg gttaaagatc cgggctcaac ccggggagtg ccactgttac tgccagacta
      121 gacgatagca ggggcaagca gaattctcgg tgtaggagtg aaatccgttg agatcgagag
      181 gaataccaat ggcgaaggca gcttgctagg ctatatgtga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003627             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003627
VERSION     KHUT01003627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c          101 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgta ggcggtcgtg
       61 caagtgaggc gtgaaagcgc ccggctcaac cgggcgagga cgcaatcgaa ctgcgcgact
      121 agaggtaggt agaggcgtgt ggaattccgg gtgtagtggt gaaatgcgta gagatccgga
      181 ggaacaccag tggcgaaggc gacacgctgg gcctgacctg acgctgagat gcgaaagcat
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003628
VERSION     KHUT01003628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           93 g           48 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtgggtgag
       61 gaagtctgct gtcaaagcgt gaggcttaac ctcataaggg cagtggaaac tcttcgtcta
      121 gagtgcgaca ggggcaaggg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccggt ggcgaaagcg ccttgctggg tctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01003629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003629
VERSION     KHUT01003629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcaac
       61 taagtcagtg gtgaaaggca gcagctcaac tgttgcactg ccattgatac tggatgactt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01003630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003630
VERSION     KHUT01003630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           79 g           58 t
ORIGIN      
        1 tacgaaggat tcgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggactaa
       61 taagtcagtg gtgaaagccc ggagctcaac tccggaactg ccattgatac tgttagtctt
      121 gagtacagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003631
VERSION     KHUT01003631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctcgg
       61 aaagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac ttccaagctc
      121 gagtcccgga gaggatggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg gccatctgga aggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003632
VERSION     KHUT01003632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 gacggaccat ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 atagttcggt gtgaaatccc tcggcttaac cgaggaatgg cgctgaatac taccgtgctc
      121 gaggaagaca gaggtaagcg gaacagcggg tggagcggtg aaatgcgttg atatccgctg
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003633
VERSION     KHUT01003633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           96 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggttttc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaagac tgggaggctg
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003634
VERSION     KHUT01003634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtgatc
       61 caagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tggattgcta
      121 gagtgcggga gaggcaagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacctgc ggtgtagacg gcttgctgga ccgccactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003635
VERSION     KHUT01003635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgaa ggcggtctgc
       61 taagtcgaac gtgaaatccc cgggctcaac ccgggaactg cgtccgatac tggcaggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgtagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003636
VERSION     KHUT01003636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggccttg
       61 taagttggac gtgaaatccc cgggcttaac tcgggtaggt cgttcaatac tgcgaggcta
      121 gaggacgtca gaggggagtg gaatccctgg tgtagcagtg aaatgcgtag atatcagggg
      181 gaacacccgt ggcgaaggcg gctccctggg acgtccctga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003637
VERSION     KHUT01003637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 taagtcccgt gtgaaatccc ctggctcaac tagggaattg cacgggagac tgcttggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003638
VERSION     KHUT01003638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           83 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaagccg acagctcaac tgtcgaactg ccattgatac tgcagggctt
      121 gagtacaaac gaggtaggcg gaattgattg tgtagcggtg aaatgcatag atacaatcaa
      181 gaacaccgat tgcgaaggca gcttactaga ttgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003639
VERSION     KHUT01003639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggaccga
       61 caagtcgggg gtgaaatccc ggggctcaac cccggaactg cctccgaaac tgtcggtctg
      121 gaggtcgaga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003640
VERSION     KHUT01003640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggccttc
       61 taagtcatgc gtgaaatccc cgggctcaac ccgggaactg cgtctgatac tggaaggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cactgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003641
VERSION     KHUT01003641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggcggctcgc
       61 taagtcggtt gtgaaatccc tgggcttaac ctgggaactg cagtcgatac tggcggacta
      121 gagtacggta gagggtggcg gaactccggg tgtagcggtg aaatgcgtag atatccggaa
      181 gaacaccgat ggcgaaggca accacctggg cctgtactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003642
VERSION     KHUT01003642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           93 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtcttct gtgaaaactt ggggctcaac cttaagcttg caggggatac gggcagacta
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgagaag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003643
VERSION     KHUT01003643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatgcc gcggctcaac cgtggaactg cctcagatac tgacggcctc
      121 gagggaggta ggggcgagcg gaactgtgag tggagcggtg aaatgcgttg atattcacag
      181 gaactccggt ggcgaaggcg gctcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003644
VERSION     KHUT01003644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgagcgta ggcggtctta
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaagtg cacccgaaac tgcaagactt
      121 gagtgtcgga gagggtgacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gtcacctgga cgataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003645
VERSION     KHUT01003645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           82 g           58 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggcggtttga
       61 taagttagat gtgaaagccc agggcttaac cctggaattg catttaagac tgtcagactt
      121 gaagtttgga gaggttagca gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaataccaat ggcgtaggca gctaactggc caaaacttga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003646             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003646
VERSION     KHUT01003646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           92 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagcgcgta ggcggcgacg
       61 taagttggaa gtgaaatccc atcggctcaa ccgtggaatc gctcccagta ctgcgttgct
      121 cgagggagat aggggcctgt ggaactgccg gtggagcggt gaaatgtgtt gatatcggcg
      181 ggaacgccag tggcgaaagc gacaggctgg accttttctg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003647
VERSION     KHUT01003647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggcggcctgt
       61 caagtcgcgt gtgaaatccc ctggcttaac cagggaagtg cgcgcgaaac tggcgggctt
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003648
VERSION     KHUT01003648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 gaagtcggat gtgaaagccc ggggctcaac ctcggaacgg catccgatac tcttgggctt
      121 gagaccggaa gaggtgagtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003649
VERSION     KHUT01003649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c          100 g           36 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 caagtcggga gtgaaagccc ggggctcaac cccggagggt ctctcgaaac tgccgagcta
      121 gagtgcggaa gaggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccgga ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003650
VERSION     KHUT01003650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggcttgt
       61 taagcgagat gtgaaaaccg ggggcttaac ccgcggcctg catctcgaac tggcaggctt
      121 gagtctggta gaggagtgtg gaattcccag tgtagcggtg aaatgcgcag atattgggag
      181 gaacaccagt ggcgaaggcg gcactctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003651
VERSION     KHUT01003651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccaga
       61 taagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tgtatggctc
      121 gagaccggaa gaggttagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003652
VERSION     KHUT01003652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           90 g           51 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatta ctgggcttaa agggtgcgta ggccggcccg
       61 caagtgcttt gtgaaatccc atggctcaac catggaactg cttggcatac tgcgggtctt
      121 gaggaaggta ggggttgcca gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaatgccgat ggtgaagacg ggcaactggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003653
VERSION     KHUT01003653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           97 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgggta ggcggcactg
       61 caagtcgact gtgaaatctc cgggcttaac ccggaaaggt cagccgatac tgcggggcta
      121 gagtgcagaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ctgacactga cgctgaactg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003654             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003654
VERSION     KHUT01003654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           46 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcgggt gtgaaaactc taggctcaac ctggagacgc cacccgatac tgcgatgact
      121 agagtccggt aggggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cggcgaaggc agcactctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01003655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003655
VERSION     KHUT01003655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           79 g           54 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaagccc tccgcttaac ggaggaactg ccattgatac tgcaaggctt
      121 gagtacagat gatgttggcg gaatatgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccgat tgcgaaggca gctgacaaaa ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003656             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003656
VERSION     KHUT01003656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           65 c          100 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggacgg
       61 cacgtcggtc gctgaaagcc cccggctcaa ccggggaacg ggcgccgaga cgaccgccct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003657             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003657
VERSION     KHUT01003657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           94 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcctc
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggaaggctt
      121 cgagtgcggg atgggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacctg tggtgtagac ggctctctgg accgtaactg acgctgagac acgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003658             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003658
VERSION     KHUT01003658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           86 g           53 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtaga gtgaaatctc cagggctcaa cccggaaact gctctgcata ctggcagact
      121 ggagtgatgc agaggtttgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcaaactgg gcattaactg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003659             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003659
VERSION     KHUT01003659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           48 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcgggt gtgaaagctc cgggctcaac tcggagaggt cactcgatac tgccatgact
      121 ggagtccggt aggggggtgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccaa tggcgaaggc agcaccctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003660
VERSION     KHUT01003660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           82 g           51 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggtcatg
       61 taagtagggt gtgaaatccc acagctcaac tgtggaactg cgctctaaac tacatgacta
      121 gagggatata gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003661
VERSION     KHUT01003661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatcctg
       61 taagtctcgt gtgaaatccc agggcttaac cctggaatgg catgagaaac tgcgggattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003662
VERSION     KHUT01003662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          100 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 taagtacggg gtgaaatctc acggctcaac cgtgaggctg cctcgtagac tgccgggctc
      121 gagcagagca gaggctagcg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gctagctggg ctcttgctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003663
VERSION     KHUT01003663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaatt
       61 caagtcatct gtgaaatctc tgggcttaac tcggaacggt cagatgatac tgctttgcta
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003664
VERSION     KHUT01003664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggacgca
       61 taagtcggat gtgaaatccc tgggcttaac ctaggaactg cattcgatac tgtgagtcta
      121 gagtatggta gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttactgga ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003665
VERSION     KHUT01003665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           73 g           63 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattga
       61 taagtcagtg gtgaaagcct gttgcttaac aacagaactg ccattgatac tgtcaatctt
      121 gaatatgttt gatgtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaag ccattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003666
VERSION     KHUT01003666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtaggg
       61 taagtctggt gtgaaatcct ggagcttaac ttcagaaatg cactggatac tgccctgcta
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgtaagcg aatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003667
VERSION     KHUT01003667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctttg
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgcagagctt
      121 gagtgtggga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gccaactgga ccactactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003668
VERSION     KHUT01003668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcctga
       61 taagtagggg gtgaaattcc gtggctcaac cacggaactg ccttctaaac tgccaggctt
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003669
VERSION     KHUT01003669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttgca
       61 caagtcgggt gtgaaaatcc ggggctcaac cccggacctg cacccgatac tgtgcgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003670
VERSION     KHUT01003670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          104 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggaccct
       61 taagtagtca gtgaaagccc ccggctcaac cggggagagt ctggctagac tgggggtctc
      121 gaggaccgca gaggagagtg gaattgctgg tggagcggtg gaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gctctctggg cttgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003671
VERSION     KHUT01003671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          101 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggttttg
       61 agagtcggct gtgaaatctc caggcctaac ctggaggggt cggccgagac cttgagactt
      121 gagggtggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccttctggg ccatacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cga
//
LOCUS       KHUT01003672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003672
VERSION     KHUT01003672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           97 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtctca
       61 taagtctgtt gtgaaatctc ccggctcaac cgggagggtg cggtggaaac tgtggggctg
      121 gagtatggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003673
VERSION     KHUT01003673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           95 g           51 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtctgt
       61 cgcgtcggga gtgaaaacca ggtgcttaac acctggcctg ctttcgatac gggcagacta
      121 gaggtactca ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg agtatcctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003674
VERSION     KHUT01003674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccttg
       61 caagtggaag gtgaaatccc tcggcttaac cgaggaactg ccttccagac tgttaggctt
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003675
VERSION     KHUT01003675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           86 g           48 t
ORIGIN      
        1 tacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggacttt
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggaagtctt
      121 gggctatcta ggggcaaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggttgctgga gatatgccga cactgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003676
VERSION     KHUT01003676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggtggctgtg
       61 taagtcggac atgaaagctc ctggcttaac tgggagaggt ggttcgaaac tgcatagctt
      121 gaggttggga gagggacgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gcgtcctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003677
VERSION     KHUT01003677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           79 g           61 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattga
       61 taagtcagtg gtgaaaacct gcagcttaac tgtagaactg ccattgatac tgtcagtctt
      121 gagtatggtc aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactgga ccaatactga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003678
VERSION     KHUT01003678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 gacgtagggg gcaagcgtta ttcggaatta ttgggcgtaa agcgctcgta ggcgggacag
       61 gaagtccgtg aagaaagacc tgggctcaac tcagggaccg gcacggatac tcttgttctt
      121 gaggcaatca gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcacctggg gttgttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003679
VERSION     KHUT01003679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           93 g           51 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcat
       61 caagtcgggg gtgaaatact tcagcttaac tggagaagtg ccttcgatac tggtgggctt
      121 gagggaggta ggggtctttg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg aaggactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003680
VERSION     KHUT01003680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 gacgaaccgt acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcggtg
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac tgcattgctt
      121 gagggagata gaggtgagtg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaagcg actcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003681
VERSION     KHUT01003681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggccagca ggcggtcatc
       61 tacgtttggg gtaacagctc ccggcttaac cgggagagcg ccttgaaaac gggatgactt
      121 gagagtcaga gagggacaca gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggc tgacatctga cgctgagggg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003682
VERSION     KHUT01003682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          100 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcttc
       61 taagtctcac cgttcaagcc cggagctcaa ctccggtccg cgggggatac tgggggacta
      121 gggacatgta ggggcaagcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccgga ggcgaaggcg gcttgctggg catggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003683
VERSION     KHUT01003683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcactt gtgaaatctc tgagcttaac tcagaacggc caagtgatac tgcagtacta
      121 gagtgcagaa ggggcaatca gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaatacctga ggtgaagacg ggttgctagg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003684
VERSION     KHUT01003684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggttttg
       61 gaagtcaggc gtgaaagccc cgggctcaac ctgggaactg cgcttgatac tctgagacta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003685
VERSION     KHUT01003685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          101 g           39 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggcggct
       61 ctagtcgggc gtgaaatccc cgggctcaac ccgggggctg cgcccgatac ggtgccgctc
      121 gaggacggga gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggc ccgtttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003686
VERSION     KHUT01003686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgt
       61 taagtcagct gtgaaatctc caagcttaac ttggaacggt cagctgatac tgatgtgcta
      121 gagtgcagaa agggcaaccg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003687
VERSION     KHUT01003687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggtctg
       61 taagtagagg gtgaaatccc agggctcaac cctggaactg ccttctagac tgcaggcctg
      121 gaggtcagga gaggcgagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003688
VERSION     KHUT01003688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttgggt gtgaaatatc ggggctcaac cccgagattg cactcaatac tgctttgctt
      121 gagtgctgga taggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagctactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003689
VERSION     KHUT01003689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctaaa
       61 caagtggcgg gtgaaatccc tcggcttaac cgaggaactg cctgccagac tgtttggctt
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003690
VERSION     KHUT01003690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttccg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgagac tgcgaagcta
      121 gggtgcggaa gagggaagcg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg gcttcctggt ccagcgccga cgttcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003691
VERSION     KHUT01003691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          101 g           38 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggagggg
       61 cgcgtggggt gtgaaagctg gtggcttaac tgccagtcgc catcccatac ggccactctg
      121 gagccttgca gagggcagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gctgcctggg caagcgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003692
VERSION     KHUT01003692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggcccca
       61 taagtcagac gtgaaatccc tcggctcaac cgaggaactg cgtctgagac tgcggggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003693
VERSION     KHUT01003693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggtttga
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacggaaac tgtcaggcta
      121 gagtgtggta gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggctactgga ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003694
VERSION     KHUT01003694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgccg
       61 taagtcggat gtgaaatctc agggctcaac cctgaaattg cattcgatac tgcggtgctt
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003695
VERSION     KHUT01003695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           73 g           68 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggctttg
       61 taagtcagtg gtgaaatctt agagcttaac tctaaaactg ccattgatac tgcttagctt
      121 gaatcaagta gaggtggatg gaataataca tgtagcggtg aaatgcttag atatgtatta
      181 gaacaccgat tgcgaaggca gttcactatg cttgtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003696             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003696
VERSION     KHUT01003696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          100 g           44 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggacggg
       61 taagtctggc gtgaaatctc caccgctcac cggggagagg ccgctggata ctgcctgtct
      121 ggagtttggc agaggaccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccaaaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003697
VERSION     KHUT01003697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttcgg
       61 caagtcggtc gtgaaagccc ttggcttaac caaggaactg cgatcgaaac tactggactt
      121 gagtatcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003698
VERSION     KHUT01003698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           94 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttgcg
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcgtggctt
      121 gagtgcggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgttactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003699
VERSION     KHUT01003699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           98 g           51 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggtggttctg
       61 tatgtctatc gtgaaagggt acagctcaac tgtagcgagt cggtagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003700
VERSION     KHUT01003700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcctcg
       61 caagttgggt gtgaaagccc tgggctcaac ccaggaagtg cacccaaaac tgcttggctt
      121 gagtaccgga ggggcttgga gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ccaagctgga cggttactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003701
VERSION     KHUT01003701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           91 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgttc
       61 taagtctgtt gtgaaatctc ccggcttaac tgggagggtg cggcggagac tggaatgcta
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccacgactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003702
VERSION     KHUT01003702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           87 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggggcgca ggcgggcctg
       61 taagtcagtg gtgaaatact tgggctcaac ccgggaactg ccattgatac tatgggcctt
      121 gagtgacgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaaa cgtatactga cgctgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003703
VERSION     KHUT01003703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatca ttgggcgtaa agggtgcgta ggcggtgcgg
       61 taagtctggt gtgaaatccc cgagcttaac tcgggacccg cgccagagac tgccgtgctc
      121 gagtgtggga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctttctgga ccactactga cgctgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003704
VERSION     KHUT01003704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgccg
       61 taagtcggat gtgaaatccc ggggcttaac cccggaactg cattcgatac tgcggtgctc
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003705
VERSION     KHUT01003705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           86 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtttgat gtgaaatctc cgagcttaac tcggaaatgg cattgaatac tactctgcta
      121 gagggtcgga ggggagactg gaattttcgg tgtagcagtg aaatgcgtgg atatcgaaag
      181 gaacaccagt ggcgaaagcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003706
VERSION     KHUT01003706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           98 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtccga
       61 taagtctgat gtgaaagccc tgggctcaac tcaggaactg cattggaaac tgacggactg
      121 gagtaccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gtttcctggc cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003707
VERSION     KHUT01003707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcttt
       61 taagtcagcg gtgaaatcct ccagcttaac tggagaactg ccattgatac tggaagactt
      121 gagtacagac gaggtaggcg gaattgacag tgtagcggtg aaatgcatag atattgtcaa
      181 gaacaccgat tgcgaaggca gcttactaga ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003708
VERSION     KHUT01003708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtctag
       61 tgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tactaaacta
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003709
VERSION     KHUT01003709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           48 t
ORIGIN      
        1 gacgaactgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcggcccgt
       61 taagtcgggg gtgaaatact tcggctcaac cggagaattg ccttcgatac tggcgggctg
      121 gaggaaggta ggggtcgcgg gaactgccag tggagcggtg aaatgcgttg atattggcag
      181 gaacgccggt ggtgaagacg cgcggctgga ccttttctga cgctgatgca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003710             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003710
VERSION     KHUT01003710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          101 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctctt
       61 taagtctggc gtgaaaggtt cagggctcaa cccgaacagt gcgctggaga ctggagagct
      121 tgaggacggg agagggaagc ggaattccgg gtgtagcggt gaaatgcgta gatatccgga
      181 ggaacaccga tggcgaaggc agcttcctgg cccgtatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003711
VERSION     KHUT01003711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggtgggtgta
       61 caagtcggaa gtgaaatccc agggctcaac cctggggctg ctcccgaaac tgtatgcctt
      121 gggactggta ggggctggta gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaatacctgt ggcgaaggcg gccagctggg ccagatccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003712
VERSION     KHUT01003712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           61 c          104 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgtt
       61 caggcccgcg gtgaaatccc ccggctcaac cggggaagtg ccgcggggac cggatgtctg
      121 gagtggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga cccttactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003713
VERSION     KHUT01003713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg catttgagac tgcacggcta
      121 gagtgtagca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ttactactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003714
VERSION     KHUT01003714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggtggccatg
       61 caagtcggag gtgaaatccc tcggctcaac tgaggaactg cctccgatac tgcacggctt
      121 gagtcccgga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctgga cgggtactga cactgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003715
VERSION     KHUT01003715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctac
       61 caagtctgtg gtgaaatcct ccagctcaac tggagaacgg ccgtggatac tgggagcctc
      121 gagggagata ggggcaggcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaactccggt ggcgaaggcg gcctgctgga tctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003716
VERSION     KHUT01003716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgt
       61 caagtcggac gtgaaatccc tcggctcaac cgaggaaccg cattcgatac tgtcgggctg
      121 gagtccggga ggggatggtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01003717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003717
VERSION     KHUT01003717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcgggagag
       61 gaagtccggt gtaaaagccc acggctcaac cgtggaagga cgctggaaac tccttttctt
      121 gagggtgata gagggaagtg gaattgccgg tgtagcggtg aaatgcgtag atatcggcag
      181 gaacacccgt ggcgaaggcg gcttcctgga tcacccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003718
VERSION     KHUT01003718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           81 g           56 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactgg
       61 taagtcagag gtgaaatctc gtcgctcaac gacgaaactg cctttgatac tatcagtctt
      121 gaatccggta gagatctgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcagattggg ccggtattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01003719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003719
VERSION     KHUT01003719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtcttg
       61 aaagtctcct gtgaaatccc cgggctcaac ccgggaagtg cggtagatac tccaagacta
      121 gagtactgga ggggttcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga cagtaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003720
VERSION     KHUT01003720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           79 g           62 t
ORIGIN      
        1 tacgtagggt gctagagttg tccggattta ctgggtgtaa agggtgctca ggcggattta
       61 taagtcagag gtgaaattcc aaagcttaac tttggagctg cctttgatac tgtgagtctg
      121 gagtttgaga gagggtaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttacctggc tcaaaactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003721
VERSION     KHUT01003721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcttg
       61 tgtgtccgct gtaaaagtac acggctcaac cgtgttaggt cggtggaaac tgcaagactt
      121 gaggctggaa ggggctggtg gaattcccca tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003722
VERSION     KHUT01003722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcgga
       61 taagtctgat gtgaaatctt cgagcttaac tcggaaactg cattggatac tattcggctg
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003723
VERSION     KHUT01003723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggacatc
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tgggtgtctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003724
VERSION     KHUT01003724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           90 g           53 t
ORIGIN      
        1 tacgtaggat gctagcgttg tccggaatca ctgggcgtaa agggtccgta ggcggtttgt
       61 taagtctcct gtgaaagttc agggctcaac cctgaaatgt cgggggatac tggcaaactt
      121 gagactgtca gaggcaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctgctggg acagttctga cgctgaggga cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01003725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003725
VERSION     KHUT01003725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgta
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catcttatac tgtatgactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003726
VERSION     KHUT01003726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcggg
       61 caagtcctgt gtgaaatccc tcggctcaac tgaggaactg cacgggaaac tgctcgactt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003727             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003727
VERSION     KHUT01003727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcggg
       61 taagtctgat gttaaagccc ggggctcaac cccggtccgc attggaaact gcctgacttg
      121 agtgcaggag aggaaagcgg tattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003728
VERSION     KHUT01003728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           85 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtaagg
       61 caagtcaggt gtgaaatctc cgggctcaac ccggaaatgg cattcgaaac taccttgcta
      121 gagggtttga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga aaactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003729
VERSION     KHUT01003729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           60 c          110 g           35 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgggc
       61 atagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcggac tggcctgcta
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003730
VERSION     KHUT01003730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggtgtaa agggcgcgta ggcggcaaga
       61 tgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg cattgcaaac tgtctggcta
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003731             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003731
VERSION     KHUT01003731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           99 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ctgggtgtaa agggtgcgta ggcggtccgt
       61 taagtctgat gtgaaagccc tgggctcaac tcaggaatgg cattggaaac tggcgggcta
      121 gaataccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cggattattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01003732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003732
VERSION     KHUT01003732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           96 g           51 t
ORIGIN      
        1 tacgtagggt acaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggttggt
       61 cacgtctgct gtggaaacgc aacgcttaac gttgcgcgtg cagtgggtac gggctgacta
      121 gagtgcagta ggggagtctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ctgtaactga cactgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003733
VERSION     KHUT01003733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgagg
       61 taagttggat gtgaaatccc ggggctcaac cccggaactg cattcaatac tgctttgcta
      121 gagtattgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctgga caataactga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003734
VERSION     KHUT01003734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcctgg
       61 taggtcagct gtgaaaactc gaggctcaac ctcgagacgc cggttgaaac catcaggcta
      121 gagtccggaa gaggagagtg gaattccagg tgtagcggtg aaatgcgcag atatctggaa
      181 gaacacccgt ggctaaggcg gctctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003735             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003735
VERSION     KHUT01003735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           85 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggcgtaa agggtgcgta ggcggtttat
       61 caagtcagag gtcaaaggct acggctcaac cgtagtaagc ctctgaaact gatgaacttg
      121 agtataggag aggaaagtgg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtg gcgaaggcga ctttctggac tataactgac gctgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01003736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003736
VERSION     KHUT01003736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          103 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcacga
       61 tcagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tatcgagctg
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003737
VERSION     KHUT01003737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctag
       61 caagtcggtt gtgaaatccc ttggcttaac caaggaactg cattcgaaac tactagacta
      121 gagtatcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003738
VERSION     KHUT01003738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggcggctcta
       61 taagtcagaa gtgaaagccc cgggcttaac tcgggaagtg cttttgatac tgcagagctt
      121 gaatgtggga gagggtagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctacctggc ccaacattga cgctgagacc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01003739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003739
VERSION     KHUT01003739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           77 g           66 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcttt
       61 taagtcagtg gtgaaatcct atagctcaac tatagatgtg ccattgatac tgatagactt
      121 gagtgaagtc gaagtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg cttatactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003740
VERSION     KHUT01003740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggtgcgg
       61 taagttaggc gtgaaatctc ccggctcaac tgggagaggt cgtctaatac taccgtacta
      121 gagagcagaa gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctggt ctgtttctga cgctcagacg cgacagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01003741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003741
VERSION     KHUT01003741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           95 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatttt gcggcttaac cgtaacagtg cctttgatac tggcgggctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003742
VERSION     KHUT01003742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           86 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctta
       61 taagtcagtg gtgaaatact ccggctcaac cggaggggtg ccattgatac tgtagggctt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gcttacttgg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01003743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003743
VERSION     KHUT01003743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           84 g           54 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaattg cgcccgaaac tgctgtgctt
      121 gaggaataca gaggtatgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcatactggg tattttctga cgctcatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003744             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003744
VERSION     KHUT01003744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           45 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgc
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cacctgatac tggtgtgact
      121 cgagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003745
VERSION     KHUT01003745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          101 g           47 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 tgcgtcgccc gtgaaaacct ggggctcaac ttcaggattg cgggcgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003746             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003746
VERSION     KHUT01003746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           51 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttgcg
       61 taagtcggat gtgaaaactc agggctcaac cctgagacgc catccgatac tgcgctgact
      121 tgaatccagt aggggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccta tggcgaaggc agcactctgg gctggtattg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003747
VERSION     KHUT01003747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           81 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggccttg
       61 taagtcagtg tttaaagtct tcagcttaac tggaggagtg gcattgatac tgcaaggctt
      121 gagtaaagtt gaggtaggcg gaattgaagg tgtagcggtg aaatgcatag atatcttcaa
      181 gaacaccgat tgcgaaggca gcttactaag ctttaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003748
VERSION     KHUT01003748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagac gtgaaaaccc tgggcttaac ccaggaactg cgtttgaaac tggagggctt
      121 gaggaccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cggcacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003749             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003749
VERSION     KHUT01003749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           64 c          111 g           33 t
ORIGIN      
        1 tacggagggc gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccctg
       61 gcaagtctgc ggtgaaagcc cggggctcaa ccccgggtcg gccgcgggta ctgccgggct
      121 ggagcactgt aggggcgggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcccgctgg gcagtggctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003750
VERSION     KHUT01003750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggccagg
       61 caggtctgtt gtgaaaacta gaggcttaac ctctagacgt cgacagaaac cgcttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003751             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003751
VERSION     KHUT01003751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           96 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcttgt
       61 acagtcaggt gtgaaattcc tgggcttaac ctgggggctg cacttgatac gtataggctt
      121 gagtagggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttttactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003752
VERSION     KHUT01003752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatggc
       61 taagtcaggg gtgaaagcct ggagctcaac tccagaattg cctttgatac tggccgtctt
      121 gagtgcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003753
VERSION     KHUT01003753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccatt
       61 taagtctggt gtgaaagccc tgggctcaac ccaggaagtg cactggatac tggatggctg
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg tccctctggc cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003754
VERSION     KHUT01003754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           85 g           52 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggattta ttgggcttaa agggtatgta ggcggcgctg
       61 tcagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac tgcagcgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgagata cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003755
VERSION     KHUT01003755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgctcgta ggtggatttg
       61 caagtctgca aggaaagacc cgggctcaac tcgggaaccc gtgtggatac tgcaaacctt
      121 gaggtagtca gaggatgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcatctggg gctaacctga cactgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003756             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003756
VERSION     KHUT01003756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttga
       61 taagtcgggt gtgaaatctc caggctcaac ctggagaggt cactcgagac tgttatgact
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtggtctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003757
VERSION     KHUT01003757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcacta
       61 caagtcactt gtgaaatctg gaggcttaac ctccagcggc caagtgaaac tgtcgagcta
      121 gagtgcagaa ggggccactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggtggctggg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003758
VERSION     KHUT01003758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 aaagtcagtg gtgaaatcct tcagctcaac tggagaagtg ccgctgatac tgacagtctc
      121 gagggaggta ggggtcgatg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003759
VERSION     KHUT01003759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaagg
       61 taagtcagat gtgaaattcc ggggctcaac cccggcactg catctgaaac tatttttctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003760
VERSION     KHUT01003760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctctg
       61 taagtcggat gtgaaatccc acggctcaac catggaagtg cattcgaaac tacagggcta
      121 gagttctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003761
VERSION     KHUT01003761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggcaagt
       61 taagtctgct gtgaaagctc ctcgcttaac ggggagaggt cggtggaaac tagctagcta
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003762
VERSION     KHUT01003762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           91 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgtgta ggcggttttt
       61 taagtctgat gtgaaatccc cgggctcaac ctgggaactg cgttggatac tggagaacta
      121 gagtacgtaa gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca accccctggg acgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003763
VERSION     KHUT01003763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcccgg
       61 caagtccgtg gtgaaagcct cctgctcaac aggagaactg ccatggatac taccgggctt
      121 gagtccaggc gaggttggcg gaatggacgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggcactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003764
VERSION     KHUT01003764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcactt gtgaaatctc agagcttaac tctgaacggt caagtgatac tgtcgtgcta
      121 gagtgcagaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ctgacactga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003765
VERSION     KHUT01003765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggctcac
       61 taagtcacac gtgaaatccc tcagcttaac tggggaactg cgtgtgaaac tggagagctt
      121 gagggtagga gaggaatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcattctgga ctatttctga cactgatgaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003766
VERSION     KHUT01003766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           86 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcaat
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgaggtg ccattgatac tggttgactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag agatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003767
VERSION     KHUT01003767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatca ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagtctgtt gtgaaagccc tgggctcaac ctgggaactg cagtggatac tggcgagcta
      121 gagtacggta gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003768
VERSION     KHUT01003768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           84 g           55 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggttttg
       61 taagtcagag gtgaaatttc ggggctcaac cccggacctg cctttgatac tgcaaagctt
      121 gtgtacggaa gaggctgatg gaatttctgg tgtagcagtg aaatgcgtag atatcagaaa
      181 gaacaccagt ggcgaaggcg gtcagctggt ccggtacaga cactaaagca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003769
VERSION     KHUT01003769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           93 g           45 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgcgta ggcgggcgtc
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ttgggcatac tggacgcctt
      121 gaggcacaca ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gctcgctggg tgtgttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003770
VERSION     KHUT01003770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgagg
       61 taagtcggat gtgaaatccc ggggctcaac cctggaactg cattcgatac tgccttgctc
      121 gaggactcga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga gagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003771
VERSION     KHUT01003771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           45 c           97 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacac tgggcttaac cgagtgcttg ctttcgatac gggcagactt
      121 gaggcattga ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggc aatgttctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003772
VERSION     KHUT01003772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           83 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggttgcg
       61 taagttagat gtgaaatccc cgggcttaac ctgggaactg cgtttaaaac tgcgtagcta
      121 gagtattgca gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctggg caaatactga cactgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003773
VERSION     KHUT01003773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcacgta ggtggtacgt
       61 caagttcggc gtgaaatctc caggctcaac ttggaggggt cgtcgaagac tggcgaacta
      121 gggtctggca gaggcaggta gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gcctgctggg ccacgaccga cactgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01003774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003774
VERSION     KHUT01003774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtctgt
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaacag cattcgatac tggcagactc
      121 gaggatggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccattactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003775             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003775
VERSION     KHUT01003775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           49 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcagct gtgaaatctc cacgctcaac gtggaggggt cagctgatac tgctgtgact
      121 tgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003776
VERSION     KHUT01003776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           89 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggccga
       61 taagtcagac gtgaaatccc tgagctcaac tcaggaactg cgtttgaaac tgccggtcta
      121 gagtggtgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003777
VERSION     KHUT01003777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttga
       61 caggtctgct gtgaaaactc gaggctcaac ctcgagatgt cggcggaaac cgtctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003778             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003778
VERSION     KHUT01003778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           47 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcag
       61 taagtcgagt gtgaaacctc caggctcaac ctggagacgc cacccgatac tgctgtgact
      121 agagtccagt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtgctctgg gctggaactg acactgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01003779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003779
VERSION     KHUT01003779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggtgtt
       61 taagttaggt gtttaatccc ggggcccaac cccggtcgtg cacctaaaac tgggcatctt
      121 gaatagtgga gaggtcagtg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccagt tgcgaaggcg gctgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003780             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003780
VERSION     KHUT01003780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           42 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggcatca ttgggcgtaa agagcgcgca ggcggcttgg
       61 taagtcggat gtgaaaaccc ggtggctcaa ccacgggcct gcatccgata ctgcccggct
      121 agaggtaggt aggggagatc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggatctctgg gccttacctg acgctcaggc gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003781
VERSION     KHUT01003781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggctttg
       61 caagtctgat gtgaaatctc caggcccaac ctggaaactg cattagaaac tgcggggcta
      121 gaggcttgga gggggaactg gaatgctcgg tgtagcagtg aaatgcgtag agatcgagcg
      181 gaacaccagt ggcgaaggcg agttcctgga caagtcctga cgctgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003782
VERSION     KHUT01003782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          102 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtggga
       61 gaagtctgtg gtgaaagctc gtggctcaac tacgagtcgg ccatggatac tcttcgactt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003783
VERSION     KHUT01003783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           93 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgat
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaatgg catttgagac tggtgagcta
      121 gagtgcggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccgttactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003784
VERSION     KHUT01003784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           96 g           41 t
ORIGIN      
        1 gacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgcgg
       61 caagtcggaa gtgaaatctc caggctcaac ttggaggctg ctcccgaaac tgccgcgcta
      121 gagtgcggga gaggccagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctggctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003785
VERSION     KHUT01003785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgt
       61 caagtcgatt gtgaaatctc cgggcttaac tcggaactgc caatcgatac tgacatgcta
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctagg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003786
VERSION     KHUT01003786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           80 g           57 t
ORIGIN      
        1 tacgtagggg gctagagttg tccggattta ctgggtgtaa agggtgctca ggcggacttg
       61 caagtcagag gtgaaatacc ggagcttaac ttcggcactg cctttgatac tgcaagtctt
      121 gagtttgaga gagggtaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttacctggc tcaaaactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003787
VERSION     KHUT01003787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtccat
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac taatagactt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003788
VERSION     KHUT01003788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttgta
       61 caagtccgat gttaaagccc ccggctcaac tgggggaggt cattggagac tgtgcgactc
      121 gagggcaaca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg acgtgctggg ttgcgactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003789
VERSION     KHUT01003789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcatga
       61 taagtcaggc gtgaaattcc cgggctcaac ctggggactg cgcttgagac tgttgagcta
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003790
VERSION     KHUT01003790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           42 c           74 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggccaaa
       61 taagtcagtg gtgaaatcct gttgcttaac aacagaattg ccattgatac tgtatggctt
      121 gagtacagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01003791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003791
VERSION     KHUT01003791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           97 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtacgta ggcggtgccg
       61 caagtccggt gtgaaatctc ctggctcaac tgggagggtg cgctggaaac tgcggtgcta
      121 gagtgcggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgt ggtgtagacg gctttctgga ccgtgactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003792
VERSION     KHUT01003792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           88 g           56 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgagc
       61 taagtttggt gtgaaatcct ggggcttaac ctcagaaatg cactgaatac tggtttgcta
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaagcg aatctctgga cagttcctga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003793
VERSION     KHUT01003793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtcata
       61 tttgtctagg gtgaaatccc ggagcttaac ttcggaagtg ccttagaaac ggtatgacta
      121 gagtcctaga gaggtttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagc agcgaaggcg gcaaactgga taggaactga cactaaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003794
VERSION     KHUT01003794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           85 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttt
       61 ttagtcagcg gtgaaagttt atggctcaac catgaaattg ccgttgatac tggggggctt
      121 gagtgccgat ggggtacatg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcgaaggca ttgtacttat cggcaactga cgctgaggca cgaaagcgcg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003795
VERSION     KHUT01003795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcttgt
       61 taagcagggt gtgaaatcct ccggctcaac cggagaacag cgctctggac tggcaggctt
      121 gagtgtgcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccggt ggcgaaagcg acacactggg gcataactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003796
VERSION     KHUT01003796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           97 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtcgtcc gtgaaaactc acagcttaac tgtgggcgtg cgggtgatac gggcaggctt
      121 gagtattgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgagaag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003797
VERSION     KHUT01003797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggtggttcgg
       61 aacgtcgtgc gtgaaagccc ccggctcaac tggggagtgt cgtgcgaaac ggtcggactg
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cactcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01003798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003798
VERSION     KHUT01003798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctttt
       61 taagtcggtg gtgaaatccc gtggctcaac tgcggaatgg ccatcgatac tggaaagctt
      121 gaatccggaa gaggtcggcg gaattactgg tgtagcggtg aaatgcgtag atatcagtag
      181 gaacaccgat ggcgaaggca gctggctgga ccggtattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003799
VERSION     KHUT01003799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcgaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcgaag
       61 aaagttgggt gtgaaagccc tgggctcaac ccaggaagtg cattcaaaac tacttcgctt
      121 gaatctcgga gggggtcaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgacctgga cgaagattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003800
VERSION     KHUT01003800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgg
       61 taggtcccgt gtcaaatcct gaggctcaac ctcagatcgt cacgggaaac cgcctcgcta
      121 gagtccggaa gaggaaagtg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaatatcgat ggcgaaggca gctttctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003801
VERSION     KHUT01003801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtccgat gtcaaagcgt gaggctcaac ctcatatagg cgttggatac tggcgagctg
      121 gacggttgga gaggaaggta gaattaccag tgtagcggtg gaatgcgtag atactggtag
      181 gaatacccat tgcgaaggca gccttctgga caacacgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01003802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003802
VERSION     KHUT01003802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           55 c          104 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgta ggtggttctg
       61 tgtgctccgc gtgaaatcgc ccggctcaac cgggcggggt cgcggatgac tacaggactg
      121 gagcctggga gggggaagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccagagctga cactgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01003803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003803
VERSION     KHUT01003803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           79 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcgttg
       61 taagtcagtg gtgaaagttt gcagcttaac tgtaaaattg ccattgatac tgcagtgctt
      121 gagtacagat gaggtgggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003804
VERSION     KHUT01003804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 tcagttcggt gtgaaagctc ggagctcaac tccgaaatgg cattgaatac tatccggctt
      121 gaggttcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga cgaattctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003805
VERSION     KHUT01003805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           90 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggtggcaagt
       61 caagtcagat gtgaaagccc ggggctcaac tccgggattg catttgaaac tggtttgcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003806
VERSION     KHUT01003806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggtgcgg
       61 caagtcagat gtgaaatccc ggggctcaac ctcggaactg catccgatac tgccggactt
      121 gagggctgga gaggtgtatg gaattcatgg tggagcagtg aaatgcgtgg atatcatgag
      181 gaacgctaat ggcgtaggca gtacactgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003807             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003807
VERSION     KHUT01003807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           46 c           98 g           54 t
ORIGIN      
        1 tacggaggtg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtagg
       61 taagtgaggg gtgaaaggct gaggctcaac ctcatgtttg tcctctcata ctgtctatct
      121 tgagtgaggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg accttaactg acgctgaggc gcgaaagctg
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003808
VERSION     KHUT01003808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          100 g           46 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agcgtgggta ggcggctctg
       61 tgcgtctgtg gtgaaatctc ccggcttacc tgggaggggg tcatggatac ggcagggctt
      121 gagggcgtga gaggcttgta gaactcccgg tggaccagtg aaatgggtag agatcgggaa
      181 gaataccagt ggtgcagacg gcaagctggc gcgtacctga cgctgagcca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003809
VERSION     KHUT01003809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           95 g           47 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgtc
       61 caagtcgggt gtgaaacctc ggggctcaac tccgagcctg cattcgatac tggacggcta
      121 gagtctggta gggggatctg gaactcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003810
VERSION     KHUT01003810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtcgtg
       61 caagtcgggt gtgaaagccc agggctcaac cctggaagtg cattcgatac tgcatggcta
      121 gagtcccgga gaggatggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003811
VERSION     KHUT01003811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           84 g           51 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg ctcggtatac tggtcgtctt
      121 gagccttcta ggggcgagtg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca actcgctggg gaagtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003812
VERSION     KHUT01003812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagaacgta ggtggcttgc
       61 taagttcggt cttaaagacc ggggctcaac cccgggaatg gactgaaaac tggcaggcta
      121 gaggatgtca gaggatggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgt ggcgaaagcg gccatctggg acattcctga cactgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01003813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003813
VERSION     KHUT01003813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ttgggcgtaa agggtgcgta ggcggtttgt
       61 taagacggat gtgaaatccc tgggctcaac ctgggaactg catacgtgac tggcagacta
      121 gagtatggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003814
VERSION     KHUT01003814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 taagtgagat gtgaaatccc cgggctcaac ctgggaactg catctcatac tgtcaggcta
      121 gagtacggta gaggggggta gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccagt ggcgaaggcg gccccctgga tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003815
VERSION     KHUT01003815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           96 g           42 t
ORIGIN      
        1 gacggagggc gcgaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggccgg
       61 taagtcgagg gtgaaattcc agggctcaac tctggaactg ccttcgaaac tgtcggccta
      121 gagtgcagga gaggaaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgatgcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003816
VERSION     KHUT01003816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcttgg
       61 aaagtttgat gtgaaagctc agggctcaac cctgaaatgg cattgaatac tatcgagctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003817
VERSION     KHUT01003817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cccttgatac tgaagagctg
      121 gattgaactt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactaag gttttaatga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003818
VERSION     KHUT01003818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           94 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccagattta ctgggcgtaa agcgcacgca ggcggatgat
       61 taagtcagct gtgaaagctc ccggctcaac tgggaagggt cggctgagac tggtcgtctg
      121 gagggcaaca gaggagtgta gaattgcggg tgtagtggtg aaatgcgtag atatccgcag
      181 gaataccagt ggcgaaggcg gcactctggg ttgtacctga cgctgatgtg cgaaggctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003819
VERSION     KHUT01003819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           76 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaactg ccattgatac tatttgtctt
      121 gaatattgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctacg catatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003820
VERSION     KHUT01003820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          106 g           42 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggcaggc
       61 caagtcggtg gtgaaagcgc ccggctcaac tgggcgaggt ccatggaaac tggtcagctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gctcgctggg ccggtcctga cgctgaaacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003821
VERSION     KHUT01003821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 cacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca gacggagcag
       61 caagtggtgt atgaaatcgc ggtgcttaac acagcgaggt tatgccagac tgctggacta
      121 gagtgcgaga gagggagatg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaagcg gtctcctggc tcgcaactga cgttcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003822
VERSION     KHUT01003822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgtatgta ggcggctccg
       61 taagtgtcgg gtgaaatccc acggcctacc cgtggaactg ttcggcatac tgcggagcta
      121 gaggatggta gaggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gctctctggt ccattactga cgctgaggta cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003823
VERSION     KHUT01003823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtctgat gtgaaatctc tgagctcaac tcagaaattg cattagaaac taccttgcta
      121 gaggattgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003824
VERSION     KHUT01003824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           98 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccttc
       61 ttagtctggt gtgaaagccc ggggctcaac cccggaagag cattggatac tggaaggctg
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003825
VERSION     KHUT01003825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           87 g           53 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggattgg
       61 taagtcaggg gtgaaatctc acggcttaac cgtgaaattg cctttgatac tgccaatctt
      121 gagttcggga gaggtcgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt agcgaaggcg gtcgactggc ccgtaactga cgctcaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003826
VERSION     KHUT01003826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           88 g           44 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgcgggtctt
      121 gagctgctta ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggtgaaaaca gacgactggg agcacgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003827             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003827
VERSION     KHUT01003827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaggta ggtggtcttg
       61 tttgtctgtt ggtgaaatcc cggggcttaa ctccggaagt gccacagaaa cggcaagact
      121 tggagtactg gagaggtgag cggaattccc ggtgtagcgg tgaaatgcgt agagatcggg
      181 aggaacacca gaggcgaagg cggctcactg gacagtcact gacactcaac tgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01003828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003828
VERSION     KHUT01003828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           93 g           39 t
ORIGIN      
        1 gacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccg
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgcgggtctt
      121 gagccggcca ggggtcaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaac ggtgaaaaca ggtgactggg gccgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003829             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003829
VERSION     KHUT01003829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          103 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacct
       61 gcaagtcgga agtgaaaggc cggggctcaa ccccggagcg gctttcgaga ctgcggacct
      121 ggagaccagt aggggttggt ggaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacacctg tggcgaaggc ggccaactgg gctggatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003830
VERSION     KHUT01003830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 gacgaagggg gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgcaa
       61 tcagtcaggc gtgaaagccc cgagctcaac ttgggaatag cgcttgatac tattgtgcta
      121 gagagcggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003831             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003831
VERSION     KHUT01003831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           47 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgagca ggcggcctgt
       61 taagtctttt gtgaaaatcc ggggctcaac cccgtgaaat gcagaagata ctggcaggct
      121 agagaccagt agaggctagc ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacacctg tggcgaaggc ggctagctgg gctggatctg acgctcatcc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003832
VERSION     KHUT01003832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           95 g           51 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggttcgc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tggcgggcta
      121 gagtgtggga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcttcctgga ccacaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003833
VERSION     KHUT01003833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           90 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggccaag
       61 taagtcaggg gtgaaaggtc acggctcaac cgtggaattg cctttgatac tgcggggctt
      121 gaatcaggtt gaggttggcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccgat tgcgaaggca gctgactaga ccttgattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003834
VERSION     KHUT01003834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggattta ttgggcgtaa agcgcgtgca ggcggttttc
       61 taagtctgat gtgaaagccc cgggctcacc ccgggaagtg cattggaaac tgggagactt
      121 gagtacggga gagggcagcg gaattcctgg tgtaggggtg aaatccgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003835
VERSION     KHUT01003835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggtccgc
       61 taagtcgcgt gtgaaatccc tcggctcaac cgaggaattg cgggcgatac tggcgggcta
      121 gagatcggga ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg actcactggc ccgacactga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003836
VERSION     KHUT01003836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           74 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcagt
       61 taagtcagtg gtgaaagtct tccgctcaac gggagaactg ccattgaaac tgattgtctt
      121 gagttattat gaagtggtta gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaataccgat tgcgaaggca gatcactaat aatatactga cgctgatgga cgaaagcgta
      241 ggtagcgaac agg
//
LOCUS       KHUT01003837             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003837
VERSION     KHUT01003837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           94 g           42 t
ORIGIN      
        1 cacgtaggga ccaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgatac tgctgtggct
      121 agagtccagt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gctggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003838
VERSION     KHUT01003838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           77 g           62 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggactaa
       61 taagtcagtg gtgaaatcct gcagcttaac tgcagaactg ccattgatac tgttagtctt
      121 gagtttggtt aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgtaggca gcttactgga ccgacactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01003839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003839
VERSION     KHUT01003839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggcggtttgg
       61 ttagtctgtt gtgaaagctc cttgcttaac ggggagaggc caacagatac tgccaggctt
      121 gagtatggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ccattactga cgctcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003840
VERSION     KHUT01003840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcag
       61 taagttactt gtgaaatctc tgagcttaac tcagaacggc caagtaatac tgcagtgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctagg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003841
VERSION     KHUT01003841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          102 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcacgt
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccatgcgaac tggcgagctg
      121 gagcatagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag atatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003842
VERSION     KHUT01003842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agagcacgta ggcgggttcg
       61 tcagccattt gtgaaagccc aaggctcaac cttggaattg caggtggaac tgcgaatctt
      121 gaatacctga gaggatgagg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacacccga ggcgaaggcg ctcgtctggc agtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003843
VERSION     KHUT01003843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           86 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcgggctag
       61 taagtcagag gtgaaagccc accgcttaac ggtggaattg cctttgatac tgctggtctt
      121 gaattaggtt gaggttagcg gaatgtggca tgtagcggtg aaatgcttag atatgccata
      181 gaacaccgat tgcgaaggca gctaactggc ccttgattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003844
VERSION     KHUT01003844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcga
       61 taagtctgtt ctgaaagtcc agggctcaac cctgggatgc ggatggatac tgtcgagctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003845
VERSION     KHUT01003845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcgcgc
       61 taagttggat gtgaaagccc tcggcttaac cgaggaagtg catacaaaac tggcgtgctt
      121 gagtacaaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gcgatctggg ttgatactga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003846
VERSION     KHUT01003846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctgat gttaaagccc ggagctcaac tccggtatgg cattggaaac tgggcagctt
      121 gagtatgata gaggttacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtaactggg tcataactga cgctgaggaa cgaaagccag
      241 ggtagcaaaa agg
//
LOCUS       KHUT01003847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003847
VERSION     KHUT01003847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggctaga
       61 aaagtcggat gtgaaattcc agggctcaac cctggagctg catttgaaac tctctggcta
      121 gagtatagtt agggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctataactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003848
VERSION     KHUT01003848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           88 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagcg gtgaaagttt caggctcaac ctggagactg ccgttgatac tggggagctt
      121 gagttacgga ggggtacatg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcgaaggca ttgtaccggc cgtcgactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003849
VERSION     KHUT01003849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           75 g           60 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggatttg
       61 caagtcagtg gtgaaatcct gcagcttaac tgtagaactg tcattgaaac tgcatatctt
      121 gagtacattt gaagcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtcactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003850
VERSION     KHUT01003850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          103 g           46 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtttga
       61 taagcatgtg gtgaaagtcc ggggctcaac cccggatctg ccgtgtggac tgtcagactc
      121 gaggactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggag
      181 gaagaccggt ggcgaaggcg gcctgctggg cagtttctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003851
VERSION     KHUT01003851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           99 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcttg
       61 tgtgtctgct gtgaaagcat acggctcaac cgtagagggt cggtggaaac tgcaagactc
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag atatagggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagtcctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003852
VERSION     KHUT01003852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           88 g           52 t
ORIGIN      
        1 tacggagtgt gctagcgtta ctcggaatca ctgggcataa agcgcacgta ggcgggattg
       61 cgtgtcggtg gtgaaatccc ccagctcaac tggggaactg ctgccgaaac tgcttttctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggc tcgtatctga cgctgaggtg cgaaagcttg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003853             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003853
VERSION     KHUT01003853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           47 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcgggt gtgaaatttc gaggcttaac ctcgaaacgc cacccgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01003854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003854
VERSION     KHUT01003854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          101 g           39 t
ORIGIN      
        1 gacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggctctc
       61 caagcccgtg gtgaaaggct ccggcccaac cggggacgtg cttcggggac tggagagctc
      121 gggcgagcta ggggtctgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acagactggg gcttggccga cgctcatgca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003855
VERSION     KHUT01003855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcttct
       61 taagtctgat gtgaaatctc ggggctcaac cccgagcggc cattggaaac tggggagctt
      121 gagtgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003856
VERSION     KHUT01003856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcccgc
       61 caagtcactg gtgaaagctc gatgcttaac atcgagtcgg ccagtgatac tggtgggctg
      121 gagcactgta gaggctcatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003857
VERSION     KHUT01003857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcttgt
       61 catgtcaggt gtgaaagccc tcggcctaac cgaggaagtg cgcctgaaac tgccaagctt
      121 gagtacgaga gaggaggacg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gttctctgga tcgatactga cactaagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003858
VERSION     KHUT01003858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           76 g           59 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgca ggcgggtata
       61 taagtcagag gtgaaattcc acggctcaac tgtgggactg cctttgatac tgcatatcta
      121 gagtatattt ggagtgggcg gaatgtagca tgtagcggtg aaatgcttag atatgctaca
      181 gaacaccgat cgtgaagaca gctcactaaa ctataactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003859
VERSION     KHUT01003859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           61 c          110 g           37 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcccgg
       61 caagcatggc gtgaaagccc ccggctcaac cggggagggt cgtcgtggac tgctgggctt
      121 gagggcggta ggggttggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccaactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003860
VERSION     KHUT01003860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtaata
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gatatgacta
      121 gagtatgtga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc acaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003861
VERSION     KHUT01003861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttagc
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgatac tggctagcta
      121 gagtttggta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctaaaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003862
VERSION     KHUT01003862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcttgg
       61 ccagtcaggc gtgaaattcc tgggcttaac ctggggactg cgcttgatac tgccaggctt
      121 gaggccgaga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg accacctggc tcggtactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003863
VERSION     KHUT01003863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggcgatctt
      121 gaggcatcta ggggcagctg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgaa ggtgaagaca agctgctggg gatgaactga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003864
VERSION     KHUT01003864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           95 g           45 t
ORIGIN      
        1 aacgtaggtc ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtgggc
       61 taagtcgggc atgaaatctc tcggctcaac cgggagaggt tgtccgatac tggcgcactt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgcccctga cgctgacacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003865
VERSION     KHUT01003865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttggt
       61 taagttggat gtgaaagccc cgggctcaac ctgggaactg catccaaaac tggccgacta
      121 gagtacagta gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg accacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003866
VERSION     KHUT01003866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttg
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg ctgggtatac tgcgagtctt
      121 gagccactta ggggctgtcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccaaa ggtgaaaaca gacagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003867
VERSION     KHUT01003867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           88 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggctgaccgg
       61 aaagtcgggc gtgaaatccc tgagctcaac tcaggaactg cactcgaaac tcccggtcta
      121 gagtggtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gcttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003868
VERSION     KHUT01003868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcgggctag
       61 taagtcaggg gtgaaagccc gcggctcaac cgcggaactg cccttgatac tgcaagtctt
      121 gagtcctgga ggggctggcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga ggtgtagacg gccagctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003869
VERSION     KHUT01003869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggagtcta ggcggtccag
       61 taagtgggat gtgaaatgct ccggctcacc cggagtactg catcccaaac tgctgagctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaagct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003870
VERSION     KHUT01003870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgta ggtgggctag
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccgttgatac tattagtctt
      121 gaatactgtg gaggctagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caagcattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003871             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003871
VERSION     KHUT01003871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta cccggattta ctgggcgtaa agcgcgtgta ggcggctggc
       61 caagcgtggt gtgaaatctt cgggcttaac ccgaaaactg cattgcgaac tggtctggct
      121 agagtgtgtg agagggtagt ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggctacctgg cacataactg acgctgagac gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01003872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003872
VERSION     KHUT01003872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 gacagaggtt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggcggag
       61 tatgtccgat gtgaaatccc ttggcttaac tgaggaagtg cattggaaac tgctctgctt
      121 gagtgcgaga gaggatagcg gaattcccag agtagaggtg aaattcgtag atactgggag
      181 gaacacctga ggcgaaggcg gctatctggt tcgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003873             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003873
VERSION     KHUT01003873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctact
       61 ttaagtttga tgtgaaagct ccgggctcaa cccggaaatg gcattgaaga ctattgagct
      121 agaggtttgg aggggggact ggaatgcttg gtgtagcagt gaaatgcgta gatatcaagc
      181 ggaacaccgg tggcgaaggc gagtccctgg acaacacctg acactgaggc acgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003874             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003874
VERSION     KHUT01003874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           79 g           53 t
ORIGIN      
        1 tacataggga gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttcga
       61 aaagtctact gtcaaatacg ttggctcaac caacggcagg cattagagta ctattgaact
      121 agagtatggt agaggtagag ggaatcccta gcgtaacagt gaaatgtgta gattttaggg
      181 agaacaccag cggcgaaggc gctctactgg gccattactg acactgagag acgaaagcta
      241 agggagcgaa aggg
//
LOCUS       KHUT01003875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003875
VERSION     KHUT01003875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          108 g           36 t
ORIGIN      
        1 gacgtaggga ccaagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggatcag
       61 cgcatcgtcg gtgaaagccc cccgctcaac gggggagggt ccggcgtgat ggctggtctg
      121 gaggcaggga gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctcgctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003876
VERSION     KHUT01003876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcgg
       61 aaagtcggat gtgaaagccc tgggcttaac ccaggaagtg cactcgaaac ttccgagctt
      121 gagttctgga gagggtggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctacctgga cagaaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003877
VERSION     KHUT01003877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 tacgaactgc gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcggag
       61 aaagtagggt gtgaaatgcc ttggctcaac caaggcacag cgctctaaac tacttcgctc
      121 gagtgagtca ggggtacgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccggt ggcgaaagcg gcgtactggg gcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003878
VERSION     KHUT01003878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcagcg
       61 tgtgtcgggc gtgaaatccc atggctcaac catggaagtg cacccgaaac tgcgcagcta
      121 gagtcctgga gaggaaggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003879
VERSION     KHUT01003879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          102 g           41 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcgggttgg
       61 taagtgggtg gtgaaagcgc tcggctaaac cgggcgagtg ccatccagac tgccagtcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003880
VERSION     KHUT01003880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          103 g           37 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccctg
       61 ttggtcgggc gtgaaatccc cgggctcaac ccgggggctg cgcccgaaac ggcggggctg
      121 gaggcaagga gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggc cttgaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003881
VERSION     KHUT01003881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           94 g           47 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgttta ggcgggtgat
       61 taagttcgct atgaaagcgc caggctaaac ctggcgaggc tggcggatac tggtcatctg
      121 gagtgagtca gaggaaggta gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaataccagt ggcgaaggcg gccttctggg actcaactga cgctgaagcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003882
VERSION     KHUT01003882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          101 g           41 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcggcg
       61 taagtgcggc gtgaaagtcc tgggctcaac ctggggacgg cgctgcagac tgtgtcgcta
      121 gagtccggga gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggc ccggaactga cgctcaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003883
VERSION     KHUT01003883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           80 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggccggg
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgctcggctt
      121 gaattactgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca ggttactacc agtttattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003884
VERSION     KHUT01003884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           84 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggttgta
       61 taagttagat gtgaaagccc cgggctcaac ctgggaactg cgtttaagac tgtatgacta
      121 gagtactgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01003885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003885
VERSION     KHUT01003885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           82 g           51 t
ORIGIN      
        1 gacgaaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtag
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac tactatgctt
      121 gaggaagata gaggtaagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacacctgt ggcgaaagcg gcttactggg tcttttctga cgctgatgca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003886
VERSION     KHUT01003886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcgag
       61 taagtctgac gtgaaatccc ttggctcaac caaggaactg cgtcttaaac tgctcggctt
      121 gagtccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01003887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003887
VERSION     KHUT01003887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           82 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgca ggcggacttg
       61 taagtcaggg gtgaaagccg gcagctcaac tgtcgaactg cctttgatac tgcaagtctt
      121 gagtgaggtt gaagtagcta gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaataccaat tgcgaaggca ggttactaag tcttaactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003888
VERSION     KHUT01003888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           95 g           41 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtcgggt gtgaaagccc ggggcctaac cccggaactg cactcgaaac tgcctcgcta
      121 gagtctcgga gagggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccacctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003889
VERSION     KHUT01003889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           82 g           49 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggccttg
       61 aaagttaggt gtgaaatccc acagctcaac tgtggaactg cgcttaaaac tacaaggcta
      121 gagggatata gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003890
VERSION     KHUT01003890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c          100 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcttgt
       61 cgcgtctgcc gtgaaagctc atggcttaac tgtgggtctg cggtggatac gggctggcta
      121 gaggtaggta ggggcaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttgctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003891
VERSION     KHUT01003891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggtggccaac
       61 taagtcatcc gtgaaatccc tcagcttaac tggggaactg cgtgtgatac tggtcggctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgtaactga cactgagaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003892
VERSION     KHUT01003892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 cacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggataca
       61 caggtcaggt gtgaaatccc acggctcaac cgtggaactg catttgaaac cgtgtgtctt
      121 gagcgaacta ggggtatgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccggt ggcgaaagcg gcatactggg gttcgactga cgctgaggca cgaaagctag
      241 ggtagcgaag agg
//
LOCUS       KHUT01003893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003893
VERSION     KHUT01003893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           94 g           43 t
ORIGIN      
        1 tacagagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgg
       61 caagtggaag gtgaaatccc tcggctcaac cgaggaactg ccctccaaac tgctaggctt
      121 gagtccggga gaggcgaatg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg attcgctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003894
VERSION     KHUT01003894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcatgca ggcggatcag
       61 tcagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tgctgatctt
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003895
VERSION     KHUT01003895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacgcg
       61 gaagtcgggt gtgaaatccc tgggctcaac ccaggaactg catccgaaac tccgtgtcta
      121 gagtgtcgga gagggacgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acgtcctgga cgacgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003896
VERSION     KHUT01003896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggtgtaa agggtgtgta ggcgggactg
       61 taagtcaggg gtgaaagccc gcagcttaac tgcggaattg cccttgatac tgcaggcctt
      121 gagtcctgga ggggctgcta gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaataccaaa ggcgtaggca ggcagctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003897
VERSION     KHUT01003897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttg
       61 taagtcggat gtgaaatccc gcggctcaac cgtggaagtg cacccgaaac tacatcgcta
      121 gaggatcgga gaggaaagtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gctttctgga cgatttctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003898
VERSION     KHUT01003898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           95 g           42 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcttcg
       61 taagtgcggg gtgaaatccc acggcttacc cgtggaactg ccccgcagac tgcggagcta
      121 gaggatggta ggggagagcg gaactccagg tggagcggtg aaatgcgtag agatctggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccattactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003899
VERSION     KHUT01003899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtccgca ggcggtgcgg
       61 caagttcgag gtgacagtgc gcggcttcac tgcgcaaggt cctcgaaaac tgccacactt
      121 gagggcttca gagggacctg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaaa ggcgcaggca gggtcctggg aagcagctga cgctcatgga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003900
VERSION     KHUT01003900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           94 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcttgg
       61 taagtcggat gtgaaatccc ggggctcaac cccgtgactg cacccgaaac tgcccggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003901
VERSION     KHUT01003901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           86 g           47 t
ORIGIN      
        1 gacgaaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcgg
       61 aaagttgggt gtgaaatccc ccggctcaac cggggaattg cgcccaaaac taccgtgcta
      121 gaggaagata gaggtgagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gctcactgga tcttttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003902
VERSION     KHUT01003902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggtgtgta ggcggaagct
       61 taagtaggaa gtgaaaggtt ggagctcaac tccaacactg ctccctatac tgggtttctt
      121 gagtctcgga gaggaagatg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gtcttctgga cgagtactga cgctgagaca cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01003903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003903
VERSION     KHUT01003903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           42 c           94 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggtgggatct
       61 taagtcatgt gtctaagtgc ggggctcaac ctcgtatggg cgcaagaaac tgggattctt
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtgtctga cactgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003904
VERSION     KHUT01003904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          107 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggacggc
       61 caagtctggg gtgaaagcac ccggctcaac tggggagtgt ccctggaaac tggcggtctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggccctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003905
VERSION     KHUT01003905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           89 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggtttct
       61 caagtcaggg gtgaaagccc ggagctcaac tccggaattg cccttgaaac tgtgaagctc
      121 gagtgcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc ccgtttctga cgctcatgca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003906
VERSION     KHUT01003906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           95 g           42 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctcag
       61 caagtcaggt gtgaaagccc tcggcttaac cgaggaagtg cgcctgaaac tacggagctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccacctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003907
VERSION     KHUT01003907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          100 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcaggg
       61 aaagtcagcg gtgaaagccc ggagctcaac tccgggtcgg ccgatgatac ttctttgctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003908
VERSION     KHUT01003908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatttg
       61 taagtccgtg gtgaaatctc taagcttaac ttggaaattg ccgtggatac tataagtctt
      121 gaatattgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caactattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003909
VERSION     KHUT01003909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          103 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcctct
       61 taagtgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccatgcaaac tggggggctt
      121 gagcattgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg caattgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003910
VERSION     KHUT01003910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgct gtgaaatccc aacgcttaac gttgggcgtg cagtgggtac gggcaggcta
      121 gagtgcggta ggggtgactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtcactggg ccgcaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01003911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003911
VERSION     KHUT01003911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcttgt
       61 cgcgtctgcc gtgaaaaccc gaggctcaac ctcgggcgtg cggtgggtac gggcaggcta
      121 gagtgtggta ggggagactg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca ggtctctggg ccattactga cactgaggag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01003912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003912
VERSION     KHUT01003912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           79 g           55 t
ORIGIN      
        1 gacgggggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgca ggtggtttcc
       61 taagtccact gttaaacctt gaggctcaac ctcaaatctg cattggaaac taggagactt
      121 gagtatagta ggggtagagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaacaccgat ggcgaaggca ctctactggg ctattactga cactcagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01003913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003913
VERSION     KHUT01003913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           66 c          108 g           30 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggctgcg
       61 tatgtgcccg gtgacagccc acggctcaac cgtgggtcgc ccgagtacac agcgcgactg
      121 gaggcaggga gagggtcgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcgacctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003914
VERSION     KHUT01003914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           94 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtgtga
       61 tgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tatcgtacta
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01003915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003915
VERSION     KHUT01003915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           79 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggaccat
       61 taagtcagag gtgaaagttt tcagcttaac tggaaaattg cctttgaaac tggcggtctt
      121 gagtatagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gctcactaaa ctataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003916
VERSION     KHUT01003916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgcat
       61 ttagtcaggc gtgaaagtcc tgggcttaac ctgggaactg cgtttgatac gggtgtgcta
      121 gaggatggaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003917
VERSION     KHUT01003917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           95 g           45 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggctttt
       61 taagtctgac gtgaaagccc aaggctcagc cttggaagtg cgccagaaac tgaaaggcta
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003918
VERSION     KHUT01003918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 tacagaggcc tcgagcgttg cgcggaatca ctgggcttaa agggtgcgta ggcggataca
       61 caagtgcctt gtgaaatccc cgggctcaac ccgggaactg cttggtagac tgtgtgtctt
      121 gaggtcggta ggggtcgctg gaacagttgg tggagcggtg aaatgcgtag atatcaactg
      181 gaacgccaaa ggcgaaggca agcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003919
VERSION     KHUT01003919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgg
       61 taagaaagat gtgaaatccc cgggctcaac ctgggaattg catttttgac tgcccggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003920
VERSION     KHUT01003920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           97 g           51 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtcttg
       61 tgtgtctacc gtgaaagcgt acggctcaac cgtatttagt cggtagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003921
VERSION     KHUT01003921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           84 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggtggatatg
       61 caagtcagat gtgaaatacc cgggctcaac tcgggtgctg catttgaaac tatatatcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cactgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003922
VERSION     KHUT01003922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          100 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagtctgtg gtgaaatccc ccggctcaac cggggaactg ccgtggatac tggaggtctc
      121 gagggaggta ggggcatgcg gaactacggg tggagcggtg aaatgcgttg atatccgtag
      181 gaactccggt ggcgaaggcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003923
VERSION     KHUT01003923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           79 g           58 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggaatgg
       61 taagtcagag gtgaaatctc atcgctcaac ggtgaaactg cctttgatac tatcattctt
      121 gagtccgata gagatctgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcagattggg tcggtactga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01003924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003924
VERSION     KHUT01003924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           55 c           84 g           46 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac taccatgctc
      121 gaggaagaca gaggtaagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgatgca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003925
VERSION     KHUT01003925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           91 g           50 t
ORIGIN      
        1 tacgaatcgt ccaaacgttg ttcggattca ctgggtttaa agggcgcgta ggcggtgcgt
       61 taagtcggcg gtgaaatact tcggctcaac cggagaattg ccgctgaaac tagcgtgctc
      121 gagggaggta gaggtgcgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacacctgt ggcgaaagcg atgcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003926
VERSION     KHUT01003926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 taagtcagat gtgaaagccc agggctcaac cctggaactg catccgatac tgccatgcta
      121 gagaactgaa gaggagtgta gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaataccaac ggcgaaggca gcactctggg cagtttctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003927
VERSION     KHUT01003927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           85 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggaagat
       61 taagttagaa gtgaaatccc cgggctcaac ctgggaatgg cttttaagac tggtcatcta
      121 gagtttgtca gaggggggtg gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaacaccgat ggcgaaggca gccccctggg ataaaactga cgctcaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003928
VERSION     KHUT01003928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcccag
       61 taagtccgct ctgaaagtca ggggctcaac ccctgaatgc gggtggatac tgctgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003929
VERSION     KHUT01003929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           88 g           47 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggtgtgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccacacta
      121 gaggactgga gaggtgactg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacactcgt ggcgtaagcg agtcactgga cagtatctga cgctgaggca cgaaggctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01003930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003930
VERSION     KHUT01003930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           93 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcccgca ggcggccaga
       61 taagtttcgg gtgaaatctc ccagctcaac tgggagaggt ctcggaaaac tgtctggctc
      121 gaggcaggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01003931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003931
VERSION     KHUT01003931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           89 g           42 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcacac
       61 caagtcggat gtgaaatccc tcggctcaac caaggaagtg catccgaaac tggcgagctt
      121 gagtacggga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggc ctagtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003932
VERSION     KHUT01003932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           36 c           75 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggatcag
       61 taagtcagtg gtgaaatctt atagcttaac tataaaactg ccgttgatac tgctggtctt
      121 gagtaaattt gaagtagctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggttactaag atttaactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003933
VERSION     KHUT01003933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           56 c           86 g           45 t
ORIGIN      
        1 gacgaaccgt acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgctg
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaactg cgctctaaac tacagtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacccgt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003934
VERSION     KHUT01003934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agagctcgta ggtggctctg
       61 taagtcggag gtgaaagacc acggctcaac cgtggaaccg ccttcgaaac tgtggagctt
      121 gaggccggga gaggagatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctgga ccggttctga cactgaggag cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01003935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003935
VERSION     KHUT01003935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           98 g           43 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcgggtcag
       61 caagtcatgc gtgaaagctc ctggctaaac tgggagaggt cggatgagac tgctggtctg
      121 gagtgtgtca gagggatgtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gcatcctggg gcacaactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003936
VERSION     KHUT01003936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 taagtctggt gtgaaatctc cgagctcaac tcggaaactg cattggaaac tattcggcta
      121 gaggattgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cactgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003937
VERSION     KHUT01003937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          106 g           38 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctggc
       61 tgcgtcggtg ctgaaagcgc caggcttaac ctggcgaggc gtgccgatac gagcgagcta
      121 gaggcaggga gagggtcgcg gaattccggg tgtagtggtg atatgcgtag agatccggag
      181 gaacgccaga gacgaaggtg gcgacctggt cctgtcctga cactgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01003938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003938
VERSION     KHUT01003938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           46 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctttg
       61 taagtttgcg gtgaaagccc ggggctcaac cccgggtctg ccgtggatac tgcaaggctt
      121 gagtactgta gaggcaggta gaatatcggg tgtagcggtg gaatgcgtag agatccgata
      181 gaagaccggt ggcgaaggcg gcctgctggg cagtaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003939
VERSION     KHUT01003939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           87 g           52 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcgggagtg
       61 caagtcagag gtgaaatctc acggctcaac cgtgaaactg cctttgatac tgcatttctt
      121 gagttaggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc cctcaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003940
VERSION     KHUT01003940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacggagact gcaagcgttg ttcggattta ctgggcgtaa agggtgcgca ggcggccggg
       61 tgtgtcgggt gtgaaatttc gaggctcaac ctcgaaactg cgctcgaaac tatccggcta
      121 gagtattgga gagggaagcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccagt ggcgaaggcg gcttcctgga caattactga cgctcaggca cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01003941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003941
VERSION     KHUT01003941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           97 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgctg
       61 taagtcaggc gtgaaatccc cgggctcaac ctgggaattg cgtttgatac tgcggcgctg
      121 gaatgcggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggc ccgtaattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003942
VERSION     KHUT01003942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggctttt
       61 caagtcagag gtgaaagccc cgggctcaac ccgggaatgg cctttgagac tggatggcta
      121 gagtgcgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacccgt ggcgaaggcg gctcactggc tcgcaactga cgctcaggca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003943
VERSION     KHUT01003943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccaat
       61 taagtagggt gtgaaaggct tcggctcaac cggagcattg cgctctagac tgattggctc
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003944
VERSION     KHUT01003944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           92 g           43 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc ccggcttaac cggggaactg ctgggtatac tggcgacctt
      121 gagcaatcca ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca gctcgctggg gattcgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003945
VERSION     KHUT01003945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggcgatt
       61 caagtcgggg gtgaaatccc agggctcaac cctggaactg ccttcgatac tgtattgcta
      121 gagtatggaa gaggtaggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcctactggt ccatcactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003946
VERSION     KHUT01003946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttgg
       61 gaagtccgtg gtgaaagctc gtggctcaac tacgagtcgg ccatggatac tcctaagctt
      121 gagtcgacta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg gtcgaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003947
VERSION     KHUT01003947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           89 g           53 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggttcgg
       61 caagttggat gtgaaagctc ctggcttaac taggagaggt cgttcaatac taccgaactt
      121 gagggcatca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg atgttcctga cgctcataca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003948
VERSION     KHUT01003948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtttcc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tggggagcta
      121 gaggatggga aaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gcttgctaga ccacccctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003949             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003949
VERSION     KHUT01003949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          106 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcttct
       61 caagcgtgcg gtgaaagcct ggggctcaac cccgggtcgg ccgtgcgaac tggggggctt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttagctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003950
VERSION     KHUT01003950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           99 g           42 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcggac gtgaaatccc tcggctcaac cgaggaactg cgtctgatac tggagggctt
      121 ggatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcatcga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003951
VERSION     KHUT01003951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggc
       61 aaagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgaaac tagtcgactc
      121 gagggaggta ggggcaattg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aattgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003952
VERSION     KHUT01003952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          105 g           36 t
ORIGIN      
        1 cacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtccat
       61 tcagtcgctg gtgaaagccc cacgctcaac gtgggaaggt ccggcgagac gggtggactg
      121 gaggcaggga gaggtcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gccgactgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003953
VERSION     KHUT01003953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           97 g           39 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcccgg
       61 taagtccgtt ctgaaagtcg gaggctcaac ctccgaaagc ggatggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003954
VERSION     KHUT01003954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcga
       61 gaagtcagct gtgaaagccc cgggcttaac ctgggaatgg cggttgaaac tctcggacta
      121 gaatctggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003955
VERSION     KHUT01003955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           79 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttta
       61 taagtcaggg gtgaaagacg gcagcttaac tgtcgcagtg cctttgatac tgtagagctt
      121 gaatgtgatt gaagatggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaaccccgat tgcgaaggca gctgtctaag tcattattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003956
VERSION     KHUT01003956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 cacgtaggag acaagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtctgg
       61 taagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaatac tgctggacta
      121 gaggatggca gaggggagtg gaattccgag tgtagcggta aaatgcatag atattcggaa
      181 gaacaccagt ggcgaaggcg gctctctggg ccatttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003957
VERSION     KHUT01003957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           88 g           46 t
ORIGIN      
        1 tacccgcagc acaagtggtg gtcattatta ttgggcctaa agcgtccgta gctggcttgt
       61 tagatgcctg ggtaaatcga tgcgctcaac gcatcgaact ccgggtagac cggcaagctt
      121 gggaccggga gaggcgtgag ctactcgagg ggtaggggta aaatcctgta atccttcggg
      181 gaggaccagt ggcgaaggcg tcacgctaga acggctccga cagtgaggga cgaagccctg
      241 gggcgcaaac cgg
//
LOCUS       KHUT01003958             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003958
VERSION     KHUT01003958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c          101 g           38 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggcctgg
       61 aaagtccagg ggtgaaatac cggggctcaa ccccgtgtag gccccggata ctgccaggct
      121 agagacacgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01003959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003959
VERSION     KHUT01003959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacagtg gtgaaatccc cgggctcaac ctgggaactg ccattgtgac tgcaaggcta
      121 gagtacggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca accccctggg cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003960
VERSION     KHUT01003960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           54 c           86 g           46 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaattg cgccctaaac taccgtgctc
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003961
VERSION     KHUT01003961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacgagggga gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtctgg
       61 caagtctaag gtgaaatccc tcggctcaac cgaggaactg ccttggaaac tgccggactt
      121 gagttccgga gaggaaagca gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaataccggt ggcgaaggcg gctttctgga cggaaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003962
VERSION     KHUT01003962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcctcc
       61 tcagtctgat gtgaaagccc tgggcttacc ccaggaagtg cattggatac tgggaggctg
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg tccctctggc cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003963
VERSION     KHUT01003963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          100 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcga
       61 caagtcactt gtgaaagccc cgggcttaac tcgggaaggt caagtgatac tgtcgggcta
      121 gagtgcagaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003964
VERSION     KHUT01003964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggtatga
       61 caagtagggt gtgaaagccc tcggcttaac cgaggaattg cgctctaaac tgtcgtactt
      121 gaggaaccta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg ggttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01003965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003965
VERSION     KHUT01003965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           77 g           60 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggattta ttgggtttaa agggagcgta ggcggtctta
       61 taagtcagtg gtgaaatctc gttgcttaac aacgaacgtg ccattgatac tgtaggactt
      121 gagtacagtt gccgttggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gctgacgaaa ctgtaactga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003966
VERSION     KHUT01003966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtgggg
       61 taagtctgat gtgaaagctc gcagctcaac tgcgaaatgg cattggaaac tacctcgcta
      121 gaggagtgga agggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cacctcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003967
VERSION     KHUT01003967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agagagtgta ggcgggatcg
       61 tatgtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tgcggttctt
      121 gagtgtcgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cgaacactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003968
VERSION     KHUT01003968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           81 g           54 t
ORIGIN      
        1 gacaggtgat gcaagtgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttac
       61 caagtctgtt gttaaaactc agggcttaac cctgatccgg caatagaaac tagtaggcta
      121 gagtacggta ggggcagagg gaattttcgg tgtagtggtg aaatacgtag atatcgagaa
      181 gaacaccaat agcgaaggca ctctgctggg ccgaaactga cattgagaga cgaaagctag
      241 gggagcgaaa agg
//
LOCUS       KHUT01003969             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003969
VERSION     KHUT01003969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ttgggcgtaa agagctcgta ggcggttcaa
       61 caagtcggct gtgaaagtcc gaggctcaac ctcggaattg cagtcgagac tgttgtgact
      121 cgagtccggt agaggagaac ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003970             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003970
VERSION     KHUT01003970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           98 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccatc
       61 gcaagtcagt tgtgaaatct ccgggcttaa ctcggaacgg tcaactgata ctgcgaggct
      121 agagtgcaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacaccgg tggcgaaggc gggttgctgg gctgacactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01003971             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003971
VERSION     KHUT01003971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcacgt gtgaaatctc cacgctcaac gtggaggggt cacgtgagac tgctatgact
      121 agagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003972
VERSION     KHUT01003972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           88 g           54 t
ORIGIN      
        1 tacgtaggtt gcgagcgtta ttcggattta ctgggcgtaa agagcaccga ggtggcttct
       61 taagttggat gtaaaatctc ctggcttaac tgggaggcgc cattcaaaac tgaggagctt
      121 gaggtcagca ggggaaggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg accttctggg ctgcacctga cactctagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003973
VERSION     KHUT01003973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgaag
       61 taagtcggat gtgaaatctc cgagctcaac tcggaaactg cattggaaac tactttgctt
      121 gagggtcgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003974
VERSION     KHUT01003974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 taagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgatcgactt
      121 gaaggcagga gagagaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acttcttggc ctgttcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01003975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003975
VERSION     KHUT01003975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           99 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcggcc
       61 acagtcagat gtgaaattcc tgggcttaac ctgggggctg catttgatac gtggttgctt
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003976
VERSION     KHUT01003976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           95 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgcg
       61 taagtctggg gtgaaagccc gttgctcaac aacggaactg ccctggaaac tgcgcggctt
      121 gagtccagac gaggttggcg gaatgggcgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctgactagg ctggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003977
VERSION     KHUT01003977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggttttc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggaaggctt
      121 gagtgccgga gaggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctatctgga cggccactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01003978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003978
VERSION     KHUT01003978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           84 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccca
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgtggggctt
      121 gagtaaagtt gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gcttactaag ctttaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01003979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003979
VERSION     KHUT01003979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggttcga
       61 taagtctttt gtgaaagctc ccggcttaac tgggagaggc caagggaaac tgtcggacta
      121 gagtgtggta gagggtgctg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaatacctat ggcgaaagca ggcacctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU3999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003980
VERSION     KHUT01003980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU3999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           46 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ctcggaatca ctgggcataa agcgcgcgta ggtggcttga
       61 taagtcggtg gtgaaatccc atggctcaac catggaactg ccgtcgatac tgtcaggctt
      121 gagggcggta ggggagagtg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggg ccgttcctga cactaaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01003981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003981
VERSION     KHUT01003981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtctgg
       61 taagttggat gtgaaagctc ctggctcaac cgggagaggt cgttcaatac tgccagactc
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccgttcctga cgctaagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003982
VERSION     KHUT01003982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           82 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggcggattga
       61 taagtcagtg gtgaaagccc caggctcaac ctgggaattg ccattgatac tgttaatctt
      121 gagtgcggtt gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcactaag ccgcaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003983
VERSION     KHUT01003983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           97 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacttg
       61 caagtctgcg gtgaaagcct ccagctcaac tggagaagtg ccgtggatac tgcgggtctc
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003984
VERSION     KHUT01003984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           80 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggtatgca ggcggatact
       61 taagtaggaa gtgaaaggtt ggagctcaac tccaacactg cttcctatac tgggtgtctt
      121 gagtggcgga gaggaagatg gaacaaatcg tgtagcggtg aaatgcgtag atatgatttg
      181 gaacaccaat ggcgaaagca atcttctgga cgcaaactga cgctgagata cgaaagctat
      241 ggtagcgaac agg
//
LOCUS       KHUT01003985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003985
VERSION     KHUT01003985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           86 g           54 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgcgctg
       61 ttagtcaggt gtgaaatccc tcagctcaac tgaggaattg cgcttgatac tgcagtgctc
      121 gagggagata ggggttagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctaactgga tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01003986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003986
VERSION     KHUT01003986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           99 g           41 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggtctct
       61 caagtctggc gtgaaagccc agggctcagc cctggaagtg cgctagaaac tgagaggctt
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003987
VERSION     KHUT01003987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           95 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtg
       61 caagtctgcg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tgcatatctt
      121 gaggagggta ggggcatctg gaacattggg tggagcggtg aaatgcgttg atatccaatg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003988
VERSION     KHUT01003988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcgtac
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggtaggctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgtaactga cactgatgaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01003989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003989
VERSION     KHUT01003989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          103 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcggcctgt
       61 taagtcgggt gtgaaagacc acggctcaac cgtgggggtg catccgaaac tggcgggctt
      121 gagtgcagga gaggggagcg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003990
VERSION     KHUT01003990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttgct
       61 gaagtccgtt gtgaaagccc cgggcttaac ctgggaatgg cagtggaaac taggcgactt
      121 gaatctggca gaggggggtg gaatgccacg tgtagcagtg aaatgcgtag agatgtggcg
      181 gaacaccgat ggcgaaggca gccccctggg ttgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003991
VERSION     KHUT01003991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           97 g           45 t
ORIGIN      
        1 aacgtaggac acgagcgtta tccggagtta ctgggcgtaa agggcgagta ggtggtgcga
       61 taagactggt gtgaaagcct tcggcttaac cgagggagga cactggtgac tgtcggactt
      121 gaggcagata gaggggtgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcaccctggg tctgacctga cactgatccg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01003992             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003992
VERSION     KHUT01003992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           90 g           46 t
ORIGIN      
        1 aacgtaggac gcaaacgtta tccggaatta ctgggcgtaa agggcgcgca ggcggttagg
       61 taagttaggc atgaaagctc cctggctaaa ccgggagaag ctgtctgata ctgccaagct
      121 agagtaaggt agaggggtgt agaattccgc gtgtagtggt gaaatgcgta gatatgcgga
      181 ggaataccag tggcgaaggc ggcaccctgg accttaactg acgctgaggc gcgaaagcat
      241 gggtagcgaa cggg
//
LOCUS       KHUT01003993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003993
VERSION     KHUT01003993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgtc
       61 cgcgtcggat gtgaaagctc agagctcaac tctgagatgc cattcgatac gggacagcta
      121 gaggtagcta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg gcttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01003994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003994
VERSION     KHUT01003994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           96 g           44 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctcgg
       61 aaagtcggtc gtgaaatccg agggctcaac cctcgaactg cgtccgatac ttccgagctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01003995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003995
VERSION     KHUT01003995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           92 g           42 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcttcg
       61 aaagctaggt gtgaaagccc cgggctcaac ctgggaactg catttaggac tgcgaagctc
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01003996             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003996
VERSION     KHUT01003996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          100 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctcat
       61 caagtgtgtg gtgaaagccc ggagcttaac tccgggtctg ccatgcaaac tggtgtggct
      121 agaggactgt agaggcaggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcctgctgg gcagtttctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01003997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003997
VERSION     KHUT01003997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           84 g           57 t
ORIGIN      
        1 tacggagggt ccgagcgtta tccggaatta ttgggtttaa agggtccgca ggcgggctga
       61 taagtcaggg gtgaaatggt gcggctcaac cgtagcactg cccttgatac tgttagtctt
      121 gagtaatggt ggagtggccg gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaacaccgat cgcgaaggca ggtcactaac catttactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01003998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003998
VERSION     KHUT01003998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           84 g           51 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttgg
       61 aaagttggat gttaaaactc ccggctcaac tgggagctgt cgttcaaaac taccagacta
      121 gaggacagta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcctcctggc ctgttcctga cactcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01003999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01003999
VERSION     KHUT01003999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           98 g           42 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggcccga
       61 taagtccaag gtgaaagccc tcggctcaac cgaggaactg ccttggatac tgtcgggctc
      121 gaggctggga gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004000
VERSION     KHUT01004000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           99 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcaag
       61 caagtccggg gtgaaagctt tcggctcaac cggaaaaggg ccttggatac tgcttgtctg
      121 gagggaggta ggggcccgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgggctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004001
VERSION     KHUT01004001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           80 g           59 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggacttt
       61 taagtcagag gtgaaatccc aaagcttaac tttggagctg cctttgatac tgagagtctt
      121 gagtttgaga gagggcaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttgcctggc tcaaaactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004002
VERSION     KHUT01004002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           92 g           47 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgca ggcgggcgtg
       61 taagtcaggg gtgaaagcca ccggcctaac cggtgaattg cccttgatac tgcacgtctt
      121 gagtcccgga gaggctatcg gaattcgtgg tgtagcggtg aaatgcgtaa atatcacgag
      181 gaacacctga tgcgtaggcg gatagctgga cgggtactga cgctcaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004003
VERSION     KHUT01004003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           91 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agagtccgta ggtggtgatt
       61 caagtcgatt gttaaagagc ggggcttaac cccgtagaag cagtggaaac tgaatcacta
      121 gagagaggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaagcg ctctactggg cctcatctga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01004004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004004
VERSION     KHUT01004004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgctg
       61 taagtcactt gtgaaatctc cgggcttaac tcggaacggt caagtgatac tgcagtgcta
      121 gagtgcggaa ggggcaatcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgaagcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004005
VERSION     KHUT01004005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtccct
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggggggctt
      121 gagtgtcgga gaggtacgcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcgtactgga cgataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004006
VERSION     KHUT01004006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           75 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtggactgt
       61 taagtcaggg gtgaaagtct gcagctcaac tgtagaattg cccttgatac tggtagtctt
      121 gagttattat gaagtggtta gaatatgtgg tgtagcggtg aaatgcatag atatcacata
      181 gaataccgat tgcgaaggca gatcactaat agtacactga cactgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004007
VERSION     KHUT01004007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           80 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagccg acagctcaac tgtcgaaccg ccattgatac tggtaatctt
      121 gagtatagtt gaggcaggcg gaatttacga tgtagcggtg aaatgcatag atatcgtaaa
      181 gaacaccgat agcgaaggca gcttgctaaa ctataactga cgctgaggca cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004008
VERSION     KHUT01004008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c          101 g           46 t
ORIGIN      
        1 gacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctga
       61 taagtcagac gtgaaaggcc tgggctcaac ctgggaattg cgtttgggac tgttgggctg
      121 gagagcggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004009
VERSION     KHUT01004009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           98 g           44 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcgggt gtgaaatccc acagctcaac tgtggaattg cactcgatac tgtcgggcta
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004010
VERSION     KHUT01004010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggatgtg
       61 caagtcggaa gtgaaatctc ggggctcaac tccgagactg cttccgatac tgcacgtctg
      121 gagaccggta gaggttggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacacctgt ggcgaaggcg gccaactggg ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004011
VERSION     KHUT01004011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           99 g           44 t
ORIGIN      
        1 tacgtaggat gctagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcaccc
       61 taagtatcct gtgaaatcca cctgctcaac gggtggggtg cagggtagac tgaggagcta
      121 gagagaggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg cctcttctga cgccgaggcg cgaaggctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004012             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004012
VERSION     KHUT01004012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           88 g           44 t
ORIGIN      
        1 tacagacggc gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttcgc
       61 gaagtctact gtcaaatccc agggctcaac cttggacagg cagtggagaa ctcgcggact
      121 agagtacggt aggggtagag ggaattcccg gtgaagcagt gaaatgcaca gagatcggga
      181 agaacaccag tggcgaaggc gctctactag gccgtgactg acactgagag acgatagcta
      241 ggggagcgaa tagg
//
LOCUS       KHUT01004013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004013
VERSION     KHUT01004013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcggttatt
       61 taagtctggt tttaaagacc ggggctcaac cccgggagtg gactggatac tggatgactt
      121 gacctctgga gagggaactg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccaat ggcgaaggca agttcctgga cagaaggtga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004014
VERSION     KHUT01004014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagac gtgaaagccc cgggcttaac ctgggaatgt cgtcttaaac tgcatagctt
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004015
VERSION     KHUT01004015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcatgta ggcggcctcg
       61 taagtgtcgg atgaaatccc acggctcacc cgtggaactg ttcggcatac tgcgaggcta
      121 gaggatggta gaggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gctctctggt ccattactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004016
VERSION     KHUT01004016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtttct
       61 taagtcaggc gtgaaaccat tcggctaaac cgggtgtagt cgtctgagac tgggagacta
      121 gagtgtggta gaggcgtgta gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccagt ggcgaaggcg gcacgctggg ccataactga cgctaagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004017
VERSION     KHUT01004017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           97 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggggtga
       61 caagtggtgt atgaaatctc cgggctaaac ctggaggggt tatgccagac tgtcattcta
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004018
VERSION     KHUT01004018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           88 g           48 t
ORIGIN      
        1 tacgaacggt ccaaacgtta ttcggaatca ctgggcttaa agcgtccgta ggcggctcgg
       61 aaagtagggt gtgaaatccc acggctcaac cgtggaattg cgctctaaac tatcgagcta
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg tcttttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004019
VERSION     KHUT01004019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcacac
       61 acagtcaggc gtgaaattcc tgggctcaac ctgggggctg cgcttgatac gtgtgagcta
      121 gaggacggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccgtaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004020
VERSION     KHUT01004020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           65 c          113 g           33 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtcccg
       61 cgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac ggcgggactt
      121 gagggcggta ggggccggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccggctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004021
VERSION     KHUT01004021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           83 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggctgcggag
       61 aaagtctggt gtgaaatttg cgagctcaac tcgcaactag cacctgatac tactttgcta
      121 gagatctgga gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag atttcgagaa
      181 gaacgctcgc tgcgaaagcg aagctctggc cagattctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004022
VERSION     KHUT01004022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc agagcttaac tctgaaactg ccgttgatac tatctgtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gcttactacg caatcattga cgctgaggca cgaaagcgtg
      241 aggatcaaac agg
//
LOCUS       KHUT01004023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004023
VERSION     KHUT01004023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaagta ggtggtctca
       61 tttgtcttgt gtgaaatcct tgggcttaac tcaagaagtg cgcaagaaac ggtgagactc
      121 gagttctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagatactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004024
VERSION     KHUT01004024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c           98 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagttcagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcagactt
      121 gagggaggta gaggagagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cctttcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004025
VERSION     KHUT01004025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           91 g           50 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgcgta ggcggtttag
       61 tgtgtgggat ttgaaagccc ggggctcaac tccggatccg gatcccaaac tgctagactc
      121 gagagtatca gaggagagcg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg gtatttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004026
VERSION     KHUT01004026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggtggccatt
       61 taagtccgct gtgaaagtca gaggctcaac ctttgaaagc cggtggatac tggatggcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cactaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004027
VERSION     KHUT01004027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           91 g           50 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agggtgcgca ggcggttttt
       61 caagtcagat gtgaaatatc ccggcttaac tgggaggggc catttgaaac tgaaagacta
      121 gagtgcagga gaggatagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctggc ctgcaactga cgctgaggca cgaaggctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004028
VERSION     KHUT01004028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           76 g           64 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtggacgat
       61 taagtcggag gtgaaatcct gcagctcaac tgtagaatgg cctttgatac tggttgtctt
      121 gaatcattgt gaagtggtta gaatatgtgg tgtagcggtg aaatgcatag atatcacata
      181 gaataccgat tgcgaaggca gatcactaac aatgtattga cactgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004029
VERSION     KHUT01004029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          105 g           38 t
ORIGIN      
        1 tacagagggc gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggca
       61 caagcgtgcg gtgaaagccc ggggctcaac tccgggtcgg ccgtgcgaac tgtgttgctc
      121 gagcatggta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ccatggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004030
VERSION     KHUT01004030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggcggttcgg
       61 taagtctgtt gtgtaagctc ccggcttaac tgggagaggt caacggaaac taccggactt
      121 gagtatagga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaggca ggtaactgga ctattactga cgctcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004031
VERSION     KHUT01004031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccaag
       61 caagtctcgg gtgaaatccc tcggctcaac cgaggaactg cctcggatac tgcttggctt
      121 gaggccggga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctgga ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004032
VERSION     KHUT01004032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggttaag
       61 taagtcactt gtgaaagcgc ccggctcaac tgggcgaggc cgtgtgagac tgcttgactt
      121 gagagaggta gaggcgaatg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg attcgctggg ccttctctga cgctcagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004033
VERSION     KHUT01004033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           79 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggcctga
       61 taagtcagtg gtgaaagctc tcagcttaac tgggaaactg ccattgaaac tgtcaggctt
      121 gagtacaatt gaagtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaaa ttgcaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004034
VERSION     KHUT01004034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggcttgt
       61 taagtgagat gttaaaacgt ggggctcaac cctgtcggtg catctcatac tggcaggcta
      121 gaggaaggca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccttttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004035
VERSION     KHUT01004035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcagtg
       61 taagtctgat gtgaaagctt cgagcttaac tcgaaaatgg cattggatac tatattgctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004036
VERSION     KHUT01004036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           54 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggcggtttag
       61 caagtcaggg gtgtaagctc taggcttaac ctagaaattg cctctgaaac tacttcactt
      121 gagtattaga ggggaagctg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaggcg ggcttctggc taattactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004037
VERSION     KHUT01004037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 aaagtcagat gtgaaatccg ggggctcaac ctccgaattg catccgatac tcccatgcta
      121 gagaactgga ggggagtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacactagt ggcgaaggcg agactctgga cagtttctga cactgatgca cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004038
VERSION     KHUT01004038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtcggat gtgaaatccc ggggctcaac cctggaactg cactcgatac tgctctgcta
      121 gagaactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004039
VERSION     KHUT01004039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggttcgt
       61 taagtcggat gtgaaaactc tgggctcaac ccagagcctg catccgatac tgacgggcta
      121 gagtgcgaga ggggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gctttctgga tcgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004040
VERSION     KHUT01004040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agagcacgta ggcggacttg
       61 ttagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tgcaagtctt
      121 gagtccgaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004041
VERSION     KHUT01004041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           99 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgca ggcggctcgt
       61 taagtctggt gtgaaagccc ggggctcacc cccggaagtg cactggatac tggcgagctc
      121 gagtcccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccctctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004042
VERSION     KHUT01004042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggctggg
       61 taagtctgct gtgaaagctc ctcgcttaac ggggagaggt cagtggaaac tactcggctt
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004043
VERSION     KHUT01004043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           73 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattag
       61 taagtcagtg gtgaaatctc tgggcttaac tcagaaactg ccattgatac tattaatctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgaatattga cactgaggca cgaaagcgtg
      241 aggatcaaac agg
//
LOCUS       KHUT01004044             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004044
VERSION     KHUT01004044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcgggt gtgaaagctc cgagctcaac tcggagaggc cactcgatac tgctgtgact
      121 agaatccagt aggggagcgt ggaactcctg gtgtagcggt gaaatgcgca gatatcagga
      181 agaacaccag cggcgaaggc ggcgctctgg gctggaattg acgctgagga gcgaaagtgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004045             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004045
VERSION     KHUT01004045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggttcag
       61 caagtcgttt gtgaaagtcc gaggctcaac ctcggaattg cagtcgaaac tgctgtgact
      121 cgagtccggt agaggagaat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004046
VERSION     KHUT01004046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggtccct
       61 taagtcagat gtgaaatccc gaggcttaac ctcgggcgtg catttgaaac tgggggacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004047
VERSION     KHUT01004047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          102 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 gcagtcggcg gtgaaagccc ggagctcaac tccgggtcgg ccgtcgatac tctcgtgctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004048
VERSION     KHUT01004048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccctg
       61 caagtcggat gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcagggctg
      121 gagtactgga gaggaaggtg gaatgctcgg tgtagaggtg aaattcgtag atatcgagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004049
VERSION     KHUT01004049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcctgt
       61 taagtcgggt gtgaaaaccc ggggctcaac cccgggcctg cattcgatac tgacaggcta
      121 gaggcaggca ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004050
VERSION     KHUT01004050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggtcagg
       61 taggtccgtt gtgaaaaccc aaggctcaac cttgggacgc cgatggaaac catctgacta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggctaaggcg gttctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004051
VERSION     KHUT01004051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggatgac
       61 caagttgggg gtgaaatccc ggggcttaac ctcggaactg cctccaaaac tggtcgtctt
      121 gagtttggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004052
VERSION     KHUT01004052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatca ctgggcttaa agcgcacgta ggcggcatcg
       61 taagtgtcgg gtgaaatccc acggctcacc cgtggaactg cccggcagac tgcgatgctc
      121 gagtgtggta ggggagaacg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggt ccacaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004053
VERSION     KHUT01004053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           86 g           57 t
ORIGIN      
        1 tacagaggtc tcaagcgtta ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 taagtctggt gtgaaatctt ggagcttaac tccgaaactg caccggatac tgcgttgctt
      121 gaggattgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004054
VERSION     KHUT01004054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtgccg
       61 cacgtccgat gtgaaagccc ggggctcaac cccggacgtg cattggatac gacggcgctg
      121 gagtcctgaa gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caggtactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004055
VERSION     KHUT01004055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           94 g           50 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcatgtgca gacggattgg
       61 taagtggtgt atgaaagcgt ccggcttaac cgggcaaggg tatgccagac tgccagtctt
      121 gagaatggga gagggatgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc ccatttctga cgttcagaca tgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004056
VERSION     KHUT01004056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           41 c           72 g           62 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggagcgta ggcggacttt
       61 taagtcagtg gtgaaagctt gcagcttaac tgtaaaattg ccattgaaac tgaaagtctc
      121 gagtatggtt gaggtgactg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca ggtcactaaa ccataactga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004057
VERSION     KHUT01004057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           89 g           58 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgtcg
       61 taagtttggc gtgaaatctc ccggcttaac tgggagggtg cgctaaagac tgcgatgctt
      121 gaggatcgga gaggaaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacacctgt ggtgtagacg gctttctgga cgattcctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004058
VERSION     KHUT01004058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agagttcgta ggcggtttct
       61 caagtctggt gttaaatacg ggagctcaac tcccgcatgg cattggaaac tgggtgacta
      121 gagtgtggta gaggttacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg cgtaactgga ccataactga cgctgaggaa cgaaagccag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004059             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004059
VERSION     KHUT01004059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           82 g           59 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgca ggcggtctta
       61 taagcgtgac gtgaaatgca gcggctcaac cgtatgatgt gcgtcgcgaa ctgtgagact
      121 tgagtgtatt cgatgtcagc ggaatttgtg gtgtagcggt gaaatgctta gatatcacga
      181 agaactccga ttgcgaaggc agctgacaag gctacaactg acgctaaagc tcgaaagtgc
      241 gggtatcgaa cagg
//
LOCUS       KHUT01004060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004060
VERSION     KHUT01004060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           89 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggaactg
       61 taagttagaa gtttaataca tgggctcaac ccgtggtctg cttttaatac tgcggttctt
      121 gagtatggga gagggcgatg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt agcgaaggcg gtcgcctggc ccaatactga cgctgaggct cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004061
VERSION     KHUT01004061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          105 g           37 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtggaaggt
       61 caagcggggc ctcaaagccc ccggctcaac cggggaagct ggccgcgaac tggccttctt
      121 gagggcgaga gaggcgggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccagt ggcgaaggcg gcccgctggc tcgcagctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004062
VERSION     KHUT01004062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tggcggtctt
      121 gggccaccta ggggtcatcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgac ggtgaaaaca gatgactggg ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004063
VERSION     KHUT01004063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggacatt
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgatac tgggtgtctt
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004064
VERSION     KHUT01004064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           97 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctatt
       61 caagtcaggc gtgaaaggcc tgggctcaac ccgggaactg cgtttgatac tggatggctt
      121 gagtacggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccatctggc ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004065
VERSION     KHUT01004065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           99 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agggttcgta ggtggtggtg
       61 cgcgcaggcc atgaaagctc ctggcttaac tgggagaggc tggactggac ggcatcactg
      121 gagggtcggc gaggcagacg gaattcccgg aggagcagta aaatgcgtag agctcgggag
      181 gaacaccggt ggcgaaagcg gtctgctgga cgatacctga cactgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004066
VERSION     KHUT01004066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           58 c           83 g           48 t
ORIGIN      
        1 taccagcacc ccgagttgtc gggacgatta ttgggcctaa agcatccgta gcctgttcta
       61 caagtcctcc gttaaatcca cttgctcaac agatgggctg cgggggatac tatagggcta
      121 ggaggcggga gaggcaaacg gtactcagtg ggtaggggta aaatccattg atctactgaa
      181 gaccaccagt ggcgaaggcg gtttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004067
VERSION     KHUT01004067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtttaag gtgaaagctc cgggctcaac ccggaaatgg ccttgaagac tatcctgctc
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004068
VERSION     KHUT01004068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggtggcggag
       61 taagttagat gtgaaatacc ggcgcttaac gtcggaattg catttaagac tgcctcgctc
      121 gagctctaga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg agtctctggc tagcagctga cactgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004069
VERSION     KHUT01004069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agcgcgcgta ggcggcttgt
       61 taagtcggat gtgaaaaccg ggggctcaac ccgcggactg catctgaaac tggcaggctt
      121 gagtctggta gaggagtgtg gaattcctag tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccagt ggcgaaggcg gcactctggg ccacgactga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004070
VERSION     KHUT01004070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaataa ctgggcgtaa agggcgcgta ggtggttgca
       61 agagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gttgtaactg
      121 gagtatggta gagggcagcg gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gctgtctgga ccaatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004071
VERSION     KHUT01004071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccata
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tgtatggctc
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cagatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004072
VERSION     KHUT01004072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           52 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtccgg
       61 taagttagat gtgaaatccc ttggcttaac caaggaactg cgtttgaaac taccggactc
      121 gagggagata gaggaaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctttctggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004073
VERSION     KHUT01004073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggatgtt
       61 taagtgtgat gttaaatcgt ggggctcaac cctgtcgttg catcgcatac tgggcatcta
      121 gaggacggca gaggcaagtg gaactcgtag tgtagcagtg aaatgcgtag atattacgaa
      181 gaacaccaat ggcgaaggca gcttgctggg ccgaccctga cgctgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004074
VERSION     KHUT01004074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agcgcttgta ggtggcttgg
       61 taagttggac gtgaaatctc ccggcttaac cgggaggggt cgttcaatac tgccgggctg
      121 gaggatgtca gagggcagcg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctgcctggg gcataactga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004075
VERSION     KHUT01004075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           88 g           53 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctttg
       61 taagttagag gtgaaagccc agggctcaac cctggaactg cctttaagac tgcaatgctt
      121 gaatcacgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctgactgga cgtgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004076
VERSION     KHUT01004076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaagc
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgatac tggcaagcta
      121 gagtcccgga gaggatggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004077
VERSION     KHUT01004077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           94 g           50 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggtggtc
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tgggccgctc
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctttctgga ccacgactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004078
VERSION     KHUT01004078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           90 g           48 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta ggtggctgtt
       61 caagtctgcc gttaaagacc ggggcttaac tccggaaaaa ctgtggaaac tgaacagcta
      121 gagtatggta ggggtaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg ccttactggg ccataactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01004079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004079
VERSION     KHUT01004079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctacc
       61 taagctagat gtgaaagccc cgggctcaac ctgggaactg catttagaac tgggtggcta
      121 gagtacagca gaggagtgtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg acactctggg ttgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004080
VERSION     KHUT01004080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           96 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggaacat
       61 taagttaggg gtgaaaggcc tgggctcaac ctgggaacgg cctctaagac tgatgatctt
      121 gagtccggaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggt ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004081
VERSION     KHUT01004081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggatgtt
       61 caagtcagat gtgaaagccc tgggctcaac ccaggaattg catttgaaac tgggcgtctt
      121 gagtgttgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga caacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004082
VERSION     KHUT01004082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggagtta ctgggcgtaa agggcgcgta ggcggcgcat
       61 caagtcaggc gtgaaagccc tgggctcaac ccgggaattg cgcttgagac tggtgtgctc
      121 gagtacggga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004083
VERSION     KHUT01004083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           50 c          105 g           45 t
ORIGIN      
        1 tacggggggg gcgagcgttg tccgaagtta ctgggcgtaa agggcgcgta ggcggggtgt
       61 cacgtccgct gtgaaagcct ggagcttaac tccagcgggt cggtggatac ggacactctt
      121 gaaggcgtta ggggaagatg gaattaccca tggagcggtg aaatgcgtag agatgggtag
      181 gaacaccggt ggcgaaggcg gtcttctggg acgtccttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004084
VERSION     KHUT01004084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcggat gtgaaatccc agggctcaac cctgggcctg cattcgatac gggcagacta
      121 gagtatggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccaaaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004085
VERSION     KHUT01004085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           80 g           57 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattaa ctgggcgtaa agggtgtgta gacggaacat
       61 taagttcgct gttaaatctt tcggcttaac cggaagcttg cggtgaaaac taatgatcta
      121 gagggtgaaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt tcactcctga cgttgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004086
VERSION     KHUT01004086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           86 g           50 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggtatca ctgggcttaa agagttcgta ggcggcccga
       61 caggtgaggt gtgaaatccc tcggctcaac cgaggaattg cgcttcaaac cgtcaggctt
      121 gagggagata gaggtgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gctcactgga tcttttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004087
VERSION     KHUT01004087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           95 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctgat
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tggtcggctg
      121 gagtatgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggc ttaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004088
VERSION     KHUT01004088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           86 g           50 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggttgcg
       61 tgtgtcaggt gtgaaatctc gaggcttaac ctcgaaattg cgcctgaaac tacgtgacta
      121 gagcattgga gagggtagcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004089
VERSION     KHUT01004089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           89 g           52 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggccttt
       61 caagtgtgga gtgaaatccc acggcttacc cgtggaactg cttcgcatac tgtttggctc
      121 gaggatggta gaggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccatttctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004090
VERSION     KHUT01004090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggagcgt
       61 taagtctggc ctgaaagccc ccggctcaac cggggaaggt ggctggaaac tggcgttctt
      121 gagggtgaca gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggg tcacacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004091
VERSION     KHUT01004091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           94 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcgctc
       61 caagttaggc gtgaaagtcc tgggctcaac ctgggaactg cgcttaagac tggagtgctt
      121 gaggatggaa gagggttgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcaacctggt ccattactga cgctgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004092
VERSION     KHUT01004092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           96 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtgtgt
       61 cgcgtctgct gtgaaaactc agggctcaac tctgagcttg cagtgggtac gggcacactt
      121 gagtgctgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004093
VERSION     KHUT01004093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggtggactgg
       61 aaggtctggt gtgaaagccc ggggctcaac cccggagggt cactggaaac caacagtctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cactgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004094
VERSION     KHUT01004094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           86 g           49 t
ORIGIN      
        1 gacgagccac ccaaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggcctgc
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaattg cgcccaaaac tagtaggctt
      121 gagggagata gaggtaagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactggg tctttactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004095
VERSION     KHUT01004095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaagcg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgcgtttctt
      121 gagccattta ggggcgagtg gaacggatgg tggagcggtg aaatgcatag atatcatccg
      181 gaacgcctgt ggtgaagacg actcgctggg aatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004096             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004096
VERSION     KHUT01004096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           81 g           51 t
ORIGIN      
        1 ccccagcgcc acaagtggca atcgcgttta ttgggcctaa agcgtccgta gcaggtcttg
       61 ttaatctctt gtgaaattgc caggcttaac ttggcagatg gcaggagaca ctgcaagaat
      121 tgagaccggg aagggttaga ggtattcctg agggagcggt aaaatgctat aatctcagga
      181 ggaccaccgg tggcgaaggc gtctaactag aacggatctg actgtgaggg acgaaagcta
      241 ggggagcaaa acgg
//
LOCUS       KHUT01004097             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004097
VERSION     KHUT01004097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           46 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gcaagtcaga agtgaaattc caccgctcaa cggtggagct gctcctgata ctgcggatct
      121 ggagaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004098
VERSION     KHUT01004098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tatcaatctt
      121 gaatatcgtg gaggtatgcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcggaggca gcatactacg cgatcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004099
VERSION     KHUT01004099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccttt
       61 caagtcgaat gtgaaagccc tgggctcaac ccaggaagtg catccgaaac tgattggctt
      121 gaatgccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cggtcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004100
VERSION     KHUT01004100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatta ttgggcgtaa agcgggcgta gggggcttgg
       61 taagtcagat gtgaaagccc agggcttaac cctggaagtg catttgatac tgctgagctt
      121 gagtgttgga gaggtcactg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg ggtgactggc caaacactga ccctgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004101
VERSION     KHUT01004101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtccgtg gtgaaatccc tgagcttaac tcgggaactg ccatggatac tatttgtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca cagttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004102
VERSION     KHUT01004102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcgggttct
       61 caagtcaggg gtgaaatccc agggctcaac cctggaactg cctttgatac tgggagcctg
      121 gagttcggga gaggttggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gccaactggc ccgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004103
VERSION     KHUT01004103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcaggc gtgaaagccc caggcttaac ctgggaactg cgtttgatac tgttaagcta
      121 gagtgtggaa ggggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg ggtagctggg ccaacactga cgctgatgcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004104
VERSION     KHUT01004104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           99 g           41 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggcctgc
       61 taagtcagac gtgaaatccc tcggctcaac cgaggaactg cgtctgagac tggcgggctc
      121 gaatccggga gagggacgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga ccggcattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004105
VERSION     KHUT01004105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattgg
       61 gaagtcagcg gtgaaatact ccgacttaat cggagaactg ccgatgatac tatcaatctt
      121 gaatacggtg gaggcgggcg gaatgcgtca tgtagtagtg aaatacttag atatgacgca
      181 gaacacctat tgcgaaggca gctcgctacg ccggcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004106
VERSION     KHUT01004106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttcgg
       61 atagtcgggt gtgaaaatcc ggggctcaac cccggacctg catccgatac ttccggactg
      121 gaggcaggta ggggagagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004107
VERSION     KHUT01004107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           75 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattgg
       61 taagtccgtg gtgaaatctc tgagcttaac tcagaaactg ccatggatac tatcagtctt
      121 gaataccgtg gaggtcagta gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaataccaat tgcgaaggca gctggctaca cgagcattga cactcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004108
VERSION     KHUT01004108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           93 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggagcgta ggcggctttt
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaatgg catccgatac tggggagcta
      121 gagtttggga gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gtcacctggc ctaaaactga cgctgaagct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004109
VERSION     KHUT01004109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           88 g           54 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggacttg
       61 taagtcaggg gtgaaatctt acggcttaac cgtgaaactg cctttgatac tgcaggtctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccgtaactga cgctcaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004110             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004110
VERSION     KHUT01004110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggctgtcgtg
       61 aaagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgaaac tgcattgact
      121 agaatctcgg agagggtagc ggaattctgg gtgtagaggt gaaattcgta gatatccaga
      181 ggaacaccgg tggcgaaggc ggctacctgg acgagtattg acgctgagat gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004111             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004111
VERSION     KHUT01004111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgta ggcggtttca
       61 taagtcgtgc gtgaaagctc cctggctcaa ccgggagagg tcgtgcgata ctgcgaaact
      121 tgagtcagac agaggcaagt agaattccgg gtgtagtggt gaaatgcgta gatatccgga
      181 ggaataccag tggcgaaggc gacttgctgg gtctgtactg acgctcagac gcgaaagcat
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004112
VERSION     KHUT01004112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           83 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggccga
       61 aaagtcagcg gtgaaatact ctcgcttaac gagggaactg ccgttgatac tgtcggtctt
      121 gaatatagtg gaggtgggcg gaatgttaca tgtagcggtg aaatgcttag atatgtaacg
      181 gaacacctat tgcgaaggca gctcgctaca ctattattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004113
VERSION     KHUT01004113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           99 g           40 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggatgcg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcgcgtctg
      121 gagccttgca gaggtcgatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtcgactggg caagtgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004114
VERSION     KHUT01004114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           95 g           50 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagttggat gtgaaaactg tgggctcaac ctgcagcctg catccaatac tgacaggcta
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagagtctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004115
VERSION     KHUT01004115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           86 g           49 t
ORIGIN      
        1 tacgtaccgt ccaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggcccag
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaattg cgctcaaaac tactgggcta
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg tctttcctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004116
VERSION     KHUT01004116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           94 g           40 t
ORIGIN      
        1 gacagaggcc acgaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggctggg
       61 tccgtccgat gtgaaagccc ccggctcaac cggggaattg catcggatac agcccggctt
      121 gaggacggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004117             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004117
VERSION     KHUT01004117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          100 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcttct
       61 taagtccgtc ggtcaaatgc cggggctcaa ccccgagcgt gccgaggata ctgggaggct
      121 ggagacatgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcatgatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004118             259 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004118
VERSION     KHUT01004118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>259
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          110 g           36 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agggtgcgta ggcggccgcg
       61 cgcgtggcgg gtgaaatctc ccggcttacc cgggaggggg cttgccagac ggcgcggctt
      121 gagggacgga gaggggcgtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgac ggcgtaggca gcgccctggc cgtgcaataa ccctgacgct gaagcacgaa
      241 agctggggga gcgaacagg
//
LOCUS       KHUT01004119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004119
VERSION     KHUT01004119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcatt
       61 taggtagggt gtgaaagccc ccggctcaac cggggaattg cgccctaaac cgggtgactg
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004120             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004120
VERSION     KHUT01004120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           99 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggtttta
       61 caagtctatg ggtgaaatgc cggggctcaa ctccgtgcgt gcccaggata ctgtgagact
      121 ggagacatgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcatggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004121
VERSION     KHUT01004121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           78 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtctag
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgctggactt
      121 gaattattgg gaagcaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttgctacc aattgattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004122
VERSION     KHUT01004122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctttt
       61 tatgtcaggt gtgaaagccc tcggcctaac cgaggaagtg cgcctgaaac tgaaaagctt
      121 gagtacgaga gagggggacg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggc ggcgaaagcg gttccctgga tcgatactga cgctaagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004123
VERSION     KHUT01004123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaagctc ggagcttaac tccgaaatgg catcggaaac tatccggctc
      121 gaggaatgga ggggagactg gaatgctcgg tgtagcagtg aaatgcgtag atatcgagcg
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004124
VERSION     KHUT01004124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           86 g           54 t
ORIGIN      
        1 tacgagggga gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtacca
       61 taagtcagat gtgaaagccc tgggcttaac ctaggatgtg catttgatac tgtggaacta
      121 gagatcgaga gaggaaagtg gaattacgag tgtagaggtg aaattcgtag atattcgtaa
      181 gaacaccagt ggcgaaggcg actttctggc tcgatactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004125
VERSION     KHUT01004125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           89 g           59 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggtggtttac
       61 caagtccgat gtgaaatctc ctggcttaac tgggagggtg cgttagatac tggtaggctt
      121 gagtgtggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgcctgt ggtgtagacg gctctctgga ccattactga cactgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004126
VERSION     KHUT01004126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ctgggcgtaa agggtgtgta gacggtacat
       61 taagttcgtc gttaaaggct ccggcctaac cggagtattg cgatgaaaac tggtgagcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004127
VERSION     KHUT01004127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           92 g           56 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cacgtcggga gtgaaatctt acagcttaac tgtgaacttg ctttcgatac gggcagactt
      121 gaggcattca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg aatgtactga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004128
VERSION     KHUT01004128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           82 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttac
       61 taagtcagtg gtgaaatatc tcagcttaac tgagagggtg ccattgatac tggtaggctt
      121 gagtacagat gaagtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004129
VERSION     KHUT01004129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           87 g           58 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttacg
       61 taagttaatt gtgaaagtcc tgggcttaac ctggggattg cagttaatac tgcgtagcta
      121 gagtgttgta gaggtaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcgat ggcgaaggca gctttctgga caaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004130
VERSION     KHUT01004130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg ccccagaaac ggtgagacta
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caggtactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004131             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004131
VERSION     KHUT01004131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           80 g           54 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gacggtctag
       61 caagttagca gtgggaaatt gaggctcaac ctcatggaag ctgctaatac tgctatactc
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgtgacg atatatggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacgttgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01004132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004132
VERSION     KHUT01004132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacgat
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaattg cactcgaaac tggtcgcctt
      121 gagtgctgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004133
VERSION     KHUT01004133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           43 a           63 c          109 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgggca ggcggcccgc
       61 tcagtcggcc gtgaaagcgc cgggctcaac tcggcgaggt cgggcgatac tggcgggctc
      121 gaggctggta gagggcggtg gaattccggg tgtagtggtg gaatgcgtag agctccggag
      181 gaacaccagt ggcgaaggcg gccgcctggg ccagtcctga cgctgagccg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01004134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004134
VERSION     KHUT01004134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggctttt
       61 tatgtcgggg gtgaaatcct ggagctcaac tccagaactg cctccgaaac tgaaaagctc
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004135
VERSION     KHUT01004135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           74 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggatatg
       61 taagtcagtg gtgaaatctt cccgcttaac gggaaaactg ccgttgatac tatatatctt
      121 gaatgctgtg gaggtaagca gaatatgtca tgtagcggtg aaatgcttag agatgaccta
      181 gaataccgat tgcgaaggca gcttactaca cagatattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004136
VERSION     KHUT01004136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacagaggtg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccacg
       61 caagtcctgg gtgaaatccc tcggctcaac cgaggaactg cctcggaaac tgcatggctc
      121 gaggccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004137             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004137
VERSION     KHUT01004137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcgctt
       61 taagttgggg gttaaaggca gtggctcaac cactgttcgc ctccaaaact ggagcgcttg
      121 agtgcagcag aggaaagtgg aattcccggt gtagcggtgg aatgcgtaga gatcgggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004138
VERSION     KHUT01004138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           92 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttagt
       61 taagtcttct gtgaaagctg cgggcttagc ctgcaaagtg cagaggaaac tggctgactt
      121 gagtattgga gagggcaggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgcctggc tgattactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004139             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004139
VERSION     KHUT01004139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           86 g           52 t
ORIGIN      
        1 gacataggtg gcaagcgtta ttcggaatta ctaggcgtaa agcgagtgta ggcggacgat
       61 taagtccgat gttaaatctc caggcttaac ttggagtcgc atcggatact ggtcgcctcg
      121 agtggggtag ggggcagcgg aattcccggt gtagcggtga aatgcgtaga tatcgggagg
      181 aacacctatg gcgaaagcag ctgcctgggc ctttactgac gctaagactc gaaagctggg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004140             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004140
VERSION     KHUT01004140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           35 c           78 g           57 t
ORIGIN      
        1 tacggggggg gcaagcgtta ttcagtatga ctgggcgtaa agggtctgta ggcggcaagt
       61 ttagttgttg gggaaatctt agggcacaac cttaaaaaac tgacaaaacg tactttgcta
      121 gagtaataaa gaggataata gaacttctgg agtagggata aaatccagag acagcagaag
      181 gaatttcatg ggcgaaagca attatctggt tattactgac gctgagagac gaaggcgtgg
      241 gtagcaaatg gg
//
LOCUS       KHUT01004141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004141
VERSION     KHUT01004141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggttgtttct
       61 taagttggat gtgaaagacc ggggctcaac cccggaacgg cattcaatac tgggagactg
      121 gagggttcga ggggagtctg gaattcacgg tgtagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagt ggcgaaggcg ggactctgga gaacacctga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004142
VERSION     KHUT01004142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggctcgc
       61 taagccacac gtgaaatccc tcagcttaac tggggaactg cgtgtgggac tggggagctt
      121 gagggtagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctacatctga cactgatgaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004143
VERSION     KHUT01004143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           92 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgtta ctgagaatca ctgggtttaa agggtgcgta ggtggtccgt
       61 taagtcagtt gtgaaatccc cgggctcaac ccgggaactg cttctgatac tggcggactt
      121 gaggccagta ggggtcacta gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaatgccggt ggtgaagacg ggtgactggg ctggttctga cactgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004144
VERSION     KHUT01004144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           90 g           48 t
ORIGIN      
        1 tacgaaccgg acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggccgcg
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaattg cgccggatac tgcgtggctc
      121 gagggagaca gaggcgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcgctggg tctcttctga cgctcatgca cgaaagctag
      241 ggtagcgaac agg
//
LOCUS       KHUT01004145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004145
VERSION     KHUT01004145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgtatggg gcaagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggtttgt
       61 taagcgagat gtgaaaacag ggggctcaac ccgctgcctg catctcgaac tggcagactt
      121 gagtctggta gaggaaagtg gaattcctag tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004146
VERSION     KHUT01004146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggttcga
       61 aaagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tggcggactt
      121 gagttgcgga gaggtgggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaataccggc ggcgaaggcg gcccactgga cgacaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004147
VERSION     KHUT01004147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttgtc
       61 taagtcagat gtgaaagccc tgggctcaac ctgggaatgg catttgaaac tggtcggcta
      121 gagtgcggta gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004148
VERSION     KHUT01004148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtatt
       61 taagtcagtg gtgaaatctc cgagctcaac tcggaaactg ccattgatac tatttatctt
      121 gaatatcgtt gaggtaggcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccgat tgcgaaggca gcttactaaa cgatcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004149
VERSION     KHUT01004149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           99 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggccgat
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaattg cactcgatac tggttggcta
      121 gagtccggga gggggtggcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt agcgaaggcg gccacctgga ccggaactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01004150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004150
VERSION     KHUT01004150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtccgt
       61 ttagtcatgt gtgaaagccc ccggctcaac cggggaactg cacatgatac tggcgggctc
      121 gaggatggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactggg ccatcactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004151
VERSION     KHUT01004151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           99 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggcttct
       61 caagtctcgc gtgaaagccc agggcttagc cctggaaggg cgctagaaac tgggaagctc
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggctactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004152
VERSION     KHUT01004152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           81 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattgc
       61 caagtccgtg gtgaaatctc tgagcttaac tcagaaactg ccgtggatac tggtagtctt
      121 gaatgccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccgat tgcgaaggca gctggctaca cgaaaattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004153
VERSION     KHUT01004153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttgt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tggcagacta
      121 gagtctggta gagggtggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gccacctgga ccaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004154
VERSION     KHUT01004154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccttc
       61 taagtctggt gtgaaagccc ggggctcacc cccggaagtg cactggatac tggaagacta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004155
VERSION     KHUT01004155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           40 c           83 g           65 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtctat
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgatggactt
      121 gaatggagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat tgcgtaggca gcttactagg ctctaattga cactgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004156
VERSION     KHUT01004156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttttg
       61 taagccagcc gtgaaatccc cgggctcaac ctgggaattg cggttggaac tgcaaggcta
      121 gagtatggta gaggggtgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg acaccctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004157
VERSION     KHUT01004157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           91 g           42 t
ORIGIN      
        1 tacggagggt gctagcgtta atcggaatca ctgggcgtaa agcgcgcgta ggcggccttg
       61 taagtcggat gtgaaatccc acggctcaac cgtggaactg cattcgaaac tactcggcta
      121 gagtacagga gagggtggcg gaattcccgg tgtaggagtg aaatccgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004158
VERSION     KHUT01004158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           93 g           48 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgagca ggcggttctt
       61 caagtcagat gtgaaatatc ccggcttaac cgggaggggc catttgaaac tgagggacta
      121 gagtggagca gaggacagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actgtctggg ctttaactga cgctgaggcg cgaaggccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004159
VERSION     KHUT01004159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           99 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agggcacgca ggtggtcgcg
       61 caagtgtgat gtgaaagcgg ctcgcttaac gagccgaggc catcgccgac tgcgtaactc
      121 gagcgcgaga gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggc tcgttgctga cactgaggtg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004160             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004160
VERSION     KHUT01004160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           63 c          101 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 tacgtcggtc gctgaaagcc cccggctcaa ccggggaagc ggcggcgata cgaccggcct
      121 ggaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004161
VERSION     KHUT01004161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggctgc
       61 caagttcggg gtgaaatcct cccgctcaac gggagaactg ccccgaatac tggcggtctc
      121 gagggaggta ggggcgtgcg gaactatggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004162
VERSION     KHUT01004162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          106 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccggg
       61 taagcgtgcg gtgaaagctt ggggctcaac cccgagtctg ccgtgcgaac tgtccggctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004163
VERSION     KHUT01004163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtccgc
       61 taagtctgat gtgaaagccc gaggcttacc ctcggaagtg cattggagac tggcggacta
      121 gagtcccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004164
VERSION     KHUT01004164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           96 g           48 t
ORIGIN      
        1 gacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcctt
       61 taagtcaggc gtgaaagccc tgggctcaac ctgggaatgg cgcttgatac tgtaggacta
      121 gagagtggga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004165
VERSION     KHUT01004165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcagac gtgaaatcct taggcttaac ctgagaactg cgtttgatac tgttgggcta
      121 gagtgtggaa ggggctacta gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004166
VERSION     KHUT01004166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           84 g           49 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ttcggaatca ctgggcgtaa agcgggtgta ggtggctagg
       61 caagttggat gtgaaagccc cgggctcaac ccgggaagtg catccaaaac tacttagctt
      121 gagtaaggga gaggatagga gaattcttgg tgtagtggtg aaatacgtag atatcaagag
      181 gaacaccggt ggcgaaggcg cctatctggc ccattactga cactcagacc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004167
VERSION     KHUT01004167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgga
       61 gaagtgagat gtgaaatccc tgggcttaac ctgggaactg catcttaaac tgactggctg
      121 gagtactgta gaggacagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004168
VERSION     KHUT01004168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggcttcg
       61 tgtgtcccat gtgaaagccc tcggctcaac cggggaactg catgggaaac tgcggagctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctcactgga ccggtactga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004169
VERSION     KHUT01004169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           98 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcacga
       61 caagtcagtg gtgaaagctc gacgctcaac gtcgagtcgg ccactgatac tgttgagctg
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004170
VERSION     KHUT01004170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           48 c           74 g           56 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcgtcctgg
       61 aaagttacgg ttgaaatctc cttgctcaac aaggaaactg gccgtaaaac tatcaggatt
      121 gagtacattt gaagcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004171
VERSION     KHUT01004171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggctcga
       61 taagtcgggt gttaaacccc caggctcaac ctggggcgcc actcgatact gtcgtggcta
      121 gagtccggta ggggagcgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagc ggcgaaggcg gcgctctggg ccggaactga cactgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004172
VERSION     KHUT01004172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggtttgc
       61 taagtcatgt gtgaaatccc tcggcttaac cgaggaacga cgcatgatac tggcaagcta
      121 gagtaccaaa gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccccctggt tggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004173             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004173
VERSION     KHUT01004173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           42 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agcgcatgta ggcggatggg
       61 tgcgtcggat cctgaaagcc cccggctcaa ccggggaagt ggggccgaaa cgacccgtct
      121 ggagggacgt agggggatct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagatcctgg acgttttctg acgctgagat gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01004174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004174
VERSION     KHUT01004174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           96 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggattta ctgggcgtaa agggcgcgta ggcggcctgg
       61 ttagtgaggc gtgaaattcc cgggcttaac ctggggactg cgcttcatac tgctgggctt
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004175
VERSION     KHUT01004175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggctcgt
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tgacaggctc
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004176
VERSION     KHUT01004176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           84 g           52 t
ORIGIN      
        1 tacagaggcc acaagcgtta gacggaatca ctgggcttaa agcgtgtgta ggcgggatgg
       61 taagtacctt gtgaaatccc acagcttaac tgtggaattg cttggtatac tgccgttctt
      121 gagtcaccta ggggcagctg gaacagatgg tggagcggtg aaatgcatag atatcatctg
      181 gaacgccaat ggtgcagaca agctgctggg ggtgtactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004177
VERSION     KHUT01004177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          103 g           37 t
ORIGIN      
        1 gacagagggg gcgagcgttg tccggagtga ctgggcgtac agcgcgtgta ggtggatgac
       61 cacgtgccgg gtgacagcgc cgtgcttaac acggcaaggc cccggtacac gagtcgtctt
      121 gaggcagtga gaggatagtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gctatctggc actgacctga cactgagacg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004178
VERSION     KHUT01004178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttgtt
       61 taagtctgtc gtgaaatccc cgggctcaac ctgggaatgg cgatggatac tggacagcta
      121 gagtgtgtca gaggatggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gccatctggg acaacactga cgctgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004179
VERSION     KHUT01004179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           97 g           45 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcgggtgga
       61 caagtggtgt atgaaatcgc caggctaaac ctggcggggt tatgccagac tgtttatctg
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004180
VERSION     KHUT01004180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           99 g           47 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggccgca
       61 tttgtcaggc gtgaaagccc cgggcttaac ctgggaactg cgtttgatac ggtgtggcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004181
VERSION     KHUT01004181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggaagtt
       61 taagttagga gtgaaacccc cgggctcaac ctggggagtg cttttaagac tggacatcta
      121 gagtttgtca gaggggggtg gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaacatcgat ggcgaaggca gccccctggg atagaactga cgctcaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004182
VERSION     KHUT01004182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaatctc cgagcttaac tcggaaatgg cattggaaac tactcggctc
      121 gaggtctgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caggtcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004183
VERSION     KHUT01004183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           98 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatta ctgggcttaa agggcgcgta ggtggtgata
       61 taagtccggg gtggaaggct gaggcttaac cttggaattg ccctggaaac tgtatcactt
      121 gagtcagcga ggggatggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaaggccggt ggcgaaggcg gccatctggc gctgaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004184
VERSION     KHUT01004184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           83 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ttgggcgtaa agggtgcgca ggcggatgta
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg cgtttgaaac tatatgacta
      121 gagtgtgata gaggggagtg gaattccatg tgtagcagtg aaatgcgtag agatgtggaa
      181 gaacaccgat ggcgaaggca gctccctgga ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004185
VERSION     KHUT01004185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtgagg
       61 taagtcggat gtgaaagccc agggctcaac cctggaagtg cactcgatac tgcttcgctt
      121 gagtactgga gaggtcggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacatctgt ggcgaaggcg gccgactgga cagatactga cgctgagaca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004186             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004186
VERSION     KHUT01004186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c          102 g           37 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agagcgcgta ggcggcgcgg
       61 tacgaccgct gtgaaagccc ccggctcaac tggggagggt cagcggtaac ggccgtgcta
      121 tgagtgcaga agagggaagt ggaactccgg gagtagcggt gaaatgcgta gagacccgga
      181 ggaacaccag tggcgaaggc ggcttcctgg tctgcgactg acgctgtggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01004187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004187
VERSION     KHUT01004187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacggggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtcctg
       61 taagtcgggt gtgaaagccc caggcttaac ctgggaagtg cactcgaaac tgtagggctt
      121 gagtatggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004188
VERSION     KHUT01004188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 gacgaaccgg acgaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggctcgg
       61 aaagttgggt gtgaaatccc acggctcaac cgtggaactg cgctcaaaac tatcgagctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcattg atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004189
VERSION     KHUT01004189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           99 g           44 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttggg
       61 caagttgagt gtgaaagctc ccggctaaac tgggagagga catttaagac tgcccggcta
      121 gaggtcggta gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggtgaagacg gcgccctggg ccgatactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004190
VERSION     KHUT01004190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggactac
       61 caagtcagtg gtgaaatcct gcggctcaac cgcagaagtg cctttgatac tggtggtctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004191
VERSION     KHUT01004191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           59 c          108 g           38 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctcgt
       61 caagcctgac gtgaaagccc ccggctcaac cggggagggt cgtcggggac tggcgagctt
      121 gaggtcggca ggggttggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccaactggg ccgaacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004192
VERSION     KHUT01004192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 taagtctgtt gtgaaatcct ccagctcaac tggagaactg caatggatac tggtcgtctc
      121 gaggaaggta ggggcatgtg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg acatgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004193
VERSION     KHUT01004193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           86 g           50 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgcacgta ggcggtctga
       61 taagtcaggg gtgaaatccc agagctcaac tctggaactg cccttgaaac tgctagactg
      121 gaatcttgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004194
VERSION     KHUT01004194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccaaa
       61 caagttggat gtgaaagccc agggctcaac cctggaagtg cacccaaaac tgtttggctt
      121 gagttccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004195
VERSION     KHUT01004195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggtgcag
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac tgctgtgctg
      121 gaggaagaca gaggcgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcgctggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004196
VERSION     KHUT01004196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacgaaggtc ccgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggttggg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgcctgactt
      121 gagtaatgga ggggtagctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagag
      181 gaacactcgt ggcgaaggcg agctactgga catttactga cgctgaggct cgaaggccag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004197             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004197
VERSION     KHUT01004197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           85 g           47 t
ORIGIN      
        1 tacagacggc gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 aaagtctact gtcaaatacc agagctcaac tttggacagg cagtagagaa cttaaaggct
      121 agagtgcggt aggggtagag ggaattcccg gtgaagcagt gaaatgcaca gagatcggga
      181 agaacaccag tggcgaaggc gctctactgg gccgcaactg acactgagag acgatagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01004198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004198
VERSION     KHUT01004198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           97 g           38 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcgtg
       61 caagtcatac gtgaaagcgc ccggctcaac cgggcgaggc cgtgtgaaac tgcgcgactg
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctgga ccgaccctga cgctcagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004199
VERSION     KHUT01004199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           90 g           44 t
ORIGIN      
        1 gacgaaccac ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 agggtgggat gtgaaatccc ccggctcaac cggggaattg cgttccaaac cgccgtgctc
      121 gaggaagata gaggtgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacacccgt ggcgaaagcg gctcactggg tcttccctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004200
VERSION     KHUT01004200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta cccggattta ctgggcgtaa agagcgcgta ggcggttcaa
       61 caagtctcct gtgaaatctt ctggcttaac tggaagaggc caggggatac tgttggactt
      121 gagggcagca gaggaagacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcaaaggcg gtcttctggg ctgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004201
VERSION     KHUT01004201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtttaa
       61 tgagtgtgat gtgaaagccc tgggctcaac ctaggaagtg catcgcaaac gattgaactg
      121 gagtatatga gagggtggtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004202
VERSION     KHUT01004202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           77 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatatt
       61 taagtcagag gtgaaatctc cgggctcaac ccggaaactg cctttgatac tatttatctt
      121 gaatttcgtt gaggttggcg gaatatatca tgtagcggtg aaatgcatag atatgatata
      181 gaacaccgat tgcgtaggca gctggctgaa cgaacattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004203
VERSION     KHUT01004203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           98 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggagtca ctgggcgtaa agggcgcgta ggcggtgcgt
       61 aaagtcaggc gtgaaagccc cgggcttaac ctgggaattg cgcttgatac tgacgtgcta
      121 gagaacggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004204
VERSION     KHUT01004204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           83 g           51 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttat
       61 taagtctact gttaaatatc agggcttaac cctgaatcgg cagtagaaac tcataagctt
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccgat ggcgaaagca ctctgctggg ccgaaactga cactcagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01004205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004205
VERSION     KHUT01004205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctctg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tacagagctc
      121 gagtcccgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004206
VERSION     KHUT01004206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           99 g           44 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggatcgg
       61 atagttgggg gtgaaagccc ggggctcaac cctggaacgg cctccaagac tgccggtctt
      121 gagttcgaga gaggtgggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcccactggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004207
VERSION     KHUT01004207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           92 g           49 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggatcaa
       61 taagtctatt ctgaaagctc ctggctaaac tgggagaggc gaatggagac tgttggtcta
      121 gagtgaggaa gaggaaagtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaagcg gctttctggt ccttaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004208
VERSION     KHUT01004208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacgaagggg actagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatttg
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgcgaatctt
      121 gagtccgata gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcccactgga tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004209
VERSION     KHUT01004209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacaggggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgctg
       61 taagtcggat gtgaaatccc ggagctcaac ttcggaactg cattcgatac tgcagtgcta
      121 gaggactgga gaggagatcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004210
VERSION     KHUT01004210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           86 g           47 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcag
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaactg cgccctaaac tactgtgctt
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004211
VERSION     KHUT01004211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcagac gtgaaatccc tcggctcaac cggggaactg cgtctgatac tgggaggctt
      121 gaatccggga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ccggcattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004212
VERSION     KHUT01004212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgag
       61 caagtccggg gtgaaagcct ccggcttaac cggagaaggg ccttggatac tgctcgtctt
      121 gagggaggta ggggcttgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acaagctggc cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004213
VERSION     KHUT01004213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgca ggcggtgtgg
       61 taagtttggt gtgaaatcct ggggctcaac cccagaattg cactgaatac tgccatgcta
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttcctga cgctcatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004214
VERSION     KHUT01004214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggggcgt
       61 taagtgtctt gtgaaatccc acggctcacc cgtggaactg ctggacatac tggcgctctt
      121 gagccactta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004215
VERSION     KHUT01004215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgctcgtctt
      121 gagccaacta ggggcgaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgat ggtgaaaaca ggtcgctggg gttgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004216
VERSION     KHUT01004216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggttggt
       61 caagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tggtcgactc
      121 gaaggcagga gagggacgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgtcctggc ctgttcttga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004217
VERSION     KHUT01004217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggctacc
       61 taagtcgatt gtgaaatccc tgggctcaac ctgggaattg cagtcgatac tgggcagctc
      121 gagttcggta gagggtgacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcgat ggcgaaggca atcacctggg cctgaactga cactgtgaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004218
VERSION     KHUT01004218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           99 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 caagtcaggg gtgaaagccc acggctcaac cgtggaagtg cctttgatac tgcgggcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004219
VERSION     KHUT01004219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggtggatcgg
       61 taagttactt ataaaagccc ctggctaaac taggggaggt tgagtgatac tgccagtctt
      121 gaatagagga gaggttgacg gaattccgcg tgtagtggtg aaatgcgtag agatgcggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ctcatattga cactcagacg cgaaggcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004220
VERSION     KHUT01004220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcga
       61 taagtctggt gtgaaaactg gaggctcaac ctccagctgt cattggatac tgtcgggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004221
VERSION     KHUT01004221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           88 g           48 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggccgcgcag
       61 atagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac ttctgcgctc
      121 gaggaaccta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg ggttttctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004222
VERSION     KHUT01004222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttgg
       61 taggtctgct gtgaaaactc ggggctcaac cccgagacgt cggtggaaac cacctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaatatccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004223
VERSION     KHUT01004223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          103 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caggcccgcg gtgaaatccc ccggctcaac cggggaagtg ccgcggggac cggcggtctg
      121 gagtgagata ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004224
VERSION     KHUT01004224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           90 g           53 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgat
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaacag cccctgatac tggttgtctt
      121 gagggaggta ggggtatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtactgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004225
VERSION     KHUT01004225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 gacgaactgc ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcgata
       61 caagtcaggg gtgaaatccg gcagctcaac tgtcgaagtg cctttgatac tgtatcgctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004226
VERSION     KHUT01004226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           87 g           45 t
ORIGIN      
        1 tacagaggcc ccgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggtcag
       61 taagcgcttt gtgaaatccc acagctcaac tgtggaaccg cagggcgaac tgctgatctt
      121 gaggaagcta gaggctggta gaacgatagg tggagcggtg gaatgcgtag agatctatcg
      181 gaatgccgaa agtgaagaca gccagctggg gctttcctga cgctaaagca cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004227
VERSION     KHUT01004227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           93 g           41 t
ORIGIN      
        1 tacaggggcc ccaagcgtta atcggaatta ctgggcttaa agggtgcgta ggcggctccg
       61 taagtgcttt gtgaaagccc tcggctcaac cgaggaacag cagggcatac tgcggagctt
      121 gaggaaggta gaggccgttg gaacgctcgg tggagcggtg gaatgcgtag agatcgagcg
      181 gaacgccaaa agtgaagaca gacggctggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004228             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004228
VERSION     KHUT01004228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           53 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgagaa gtgaaatctc cagggctcaa cccggaaact gcttttcata ctggcagact
      121 tgagggatgc agaggtgaat ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc agttcactgg gcatcatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004229
VERSION     KHUT01004229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaagccc cgggctcaac ctgggaagtg cactcgaaac tgcttggctt
      121 gagtactgga gagggaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004230
VERSION     KHUT01004230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           95 g           51 t
ORIGIN      
        1 tacgtaggac ccgagcgttg tccggattta ctgggtataa agggtgcgta ggcggtcttg
       61 tgcgtcagag gtgaaatctc cgggcttaac ccggagggtg cctttgatac ggcaggactt
      121 gagtgcggga gagggtgatg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gtcacctggc ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004231
VERSION     KHUT01004231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcttgc gtgaaatcct gcggctcaac cgcaggcctg cgcgggatac tgcctcgcta
      121 gagtccggaa gaggagagtg gaattcccag tgtagcggtg gaatgcgcag atattgggag
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004232
VERSION     KHUT01004232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggttggg
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccgggaac tgcctggctg
      121 gagtgcggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggtgaagacg gctctctaga ccgcaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004233
VERSION     KHUT01004233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c          104 g           37 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggcccgc
       61 caagtctgcc gtgaaagccc agggctcagc cctggaaggg cggtagaaac tggggggctg
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004234
VERSION     KHUT01004234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggcttca
       61 taagtcagag gtgaaacctt ggggctcaac cccaagcctg cctctgaaac tgcgaagctt
      121 gagtgctcta gagggtagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg actacctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004235
VERSION     KHUT01004235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgtgg
       61 taagtggggt gtgaaatccc gaggcttaac ctcgggactg cattccatac tgccatgctt
      121 gaggactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag agatcatgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004236             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004236
VERSION     KHUT01004236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           85 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcccga
       61 taagtcagtc gtgaaatcgc tgggctcaac ccatgcaatt gcgattgata ctgttggact
      121 tgaattaggt tgaggctggc ggaatgcgac atgtagcggt gaaatgctta gatatgtcgc
      181 agaacaccga ttgcgaaggc agccagctgg gcctatattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004237
VERSION     KHUT01004237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           92 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcctcg
       61 caagtccgct gtaaaagtca aaggctcaac ctttgaatgc cagtggaaac tgtgaggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004238             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004238
VERSION     KHUT01004238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           91 g           42 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgggtcacgg gtgaaatccc ccagcttaac tggggaactg cccttgatac cactgcggct
      121 cgaggaagga aggggcaatc ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgccgg tggcgaaagc ggattgctgg tccttatctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01004239             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004239
VERSION     KHUT01004239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggattca ttgggcgtaa agggctagta ggcggcttag
       61 caagtcgggt gtgaaaactt caggctcaac ctggagcggc catccgatac tgctatggct
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggaactg acgctgaata gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01004240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004240
VERSION     KHUT01004240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           83 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcct
       61 taagtcagtg gtgaaataca gccgcttaac ggttgaggtg ccattgatac tggggaactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaga ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004241
VERSION     KHUT01004241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatca ttgggcgtaa agggcgcgta ggcgattcgg
       61 taagtctcat gtgaaagccc atagcctaac tatggaagtg catgagatac tgtcgaattt
      121 gagttcagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004242
VERSION     KHUT01004242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggtgcgt
       61 taagtcggat gtgaaatctc acagcctaac tgtgataggt cattcgaaac tggcgtgcta
      121 gaggactgga gaggagactg gaatagtcgg tgtagcggtg aaatgcgtag agatcgacta
      181 gaacaccggt ggcgaaggcg agtctctgga cagttcctga cgctcaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004243
VERSION     KHUT01004243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctttg
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg cctcggatac tgcagagctt
      121 gagggaggta ggggcggatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004244
VERSION     KHUT01004244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggatact
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctttgatac tgggtgtctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004245
VERSION     KHUT01004245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           87 g           49 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agagtgcgta ggcggcttat
       61 caggcggggt gtgaaatccc tcggcccaac cgaggaattg cgctccgaac cgataggctt
      121 gaggaggata gaggtaagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacacctgt ggcgaaagcg gcttactggg tcctttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004246
VERSION     KHUT01004246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttatg
       61 ccagtcggat gtgaaagccc cgggcttaac ctgggaattg catccgatac tgcatggcta
      121 gagtctggga gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg acttcctggc ctaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004247
VERSION     KHUT01004247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcttgt
       61 taagttggtt gtgaaaaccc atggctcaac catggaaggg cgtccaaaac tggcaagcta
      121 gagtcccgac gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg agggcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004248
VERSION     KHUT01004248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           42 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccct
       61 tttgtcgcgt gtgaaagccc cgggctcaac ctgggaacgg cgcgcgaaac gggggggctt
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004249
VERSION     KHUT01004249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           99 g           44 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggattga ctgggcgtaa agggcgtgta ggcggtttgt
       61 caagtcgggt gtgaaagcct ggggctcagc cccagaagtg catccgaaac tagcaagcta
      121 gagcgcgata gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccctctgga tcgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004250
VERSION     KHUT01004250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggcgtaa agcgcgtgta ggcggttgcg
       61 caagttagtt gtgaaatctc ccggctcaac cgggaaactg cagctaatac tgcgtggcta
      121 gagtggcaga gaggaaagtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg actttctggc tgctaactga cgctgagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004251
VERSION     KHUT01004251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 gacgtagggg gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggacttg
       61 taagtcgagg ttgaaatatc cgggctcaac tcggggcacg gccttgatac tgcaagtctt
      121 gagtcaagta gagggtaatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttacctggg cttgttctga cgctgaggca caaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004252
VERSION     KHUT01004252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c          101 g           50 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 tgcgtcaatc gtgaaaacct actgcttaac ggtgggcttg cggttgatac gggcgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004253
VERSION     KHUT01004253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttaggt gtgaaagccc cgggcttaac ctgggaattg cgcctaatac tgttaagcta
      121 gagtactgta gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga cagatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004254
VERSION     KHUT01004254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtcttg
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tgcgaggcta
      121 gagtactgga gaggttcgtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gcgaactgga cagatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004255
VERSION     KHUT01004255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ttcggaatta ttgggcgtaa agcgtgcgta ggtggtttct
       61 taagtccgat gtgaaagccc atggctcaac catggagggt cattggaaac tgagaagctt
      121 gagtacgaaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt tcgatactga cactgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004256
VERSION     KHUT01004256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           88 g           48 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgcctgtctt
      121 gagtcatcta ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctcgctggg gatgtactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004257
VERSION     KHUT01004257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggtttgc
       61 taagtcaggt gtgaaatccc caggctcaac ctgggaactg catttgatac tggcggacta
      121 gagtgtggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga ctaacactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004258
VERSION     KHUT01004258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgaggggg gcaagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggctct
       61 taagttggat gtttaaaacc ggggcccaac cccggcgtgg catccaaaac tgggagtctt
      121 gaatggaaca gaggctagtg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacacccgt tgcgaaggcg gctagctggg ttcacattga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004259
VERSION     KHUT01004259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttagg
       61 taagtggaat gtgaaatccc ccggcttacc cggggacctg catcccatac tgcctagctt
      121 gagtacggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccgatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004260
VERSION     KHUT01004260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           87 g           54 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtccat
       61 taagtgtgaa gctaaatctc ttggcttaac caagaaacta tttcgcatac tgatgggctg
      121 gagtgatgca gaggtggata gaattcccgg tgtagcggtg aaatgcgttg atatcgggag
      181 gaatacccgt ggcgaaggcg gtccactggg catcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004261
VERSION     KHUT01004261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtata
       61 taagtcagtg gtgaaagctc cgagcttaac ttggaaactg ccattgatac tgtatatctt
      121 gaatgtcgtt gaggttagcg gaatgttgca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccaat tgtgaagaca gctaactaag cgatgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004262
VERSION     KHUT01004262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           96 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tggggtgctg
      121 gagcgcggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctttctgga ccgtagctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004263             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004263
VERSION     KHUT01004263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           87 g           57 t
ORIGIN      
        1 tacgtaggtc ccgagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttca
       61 taagtctgaa gttaaaggca gtggcttaac cattgtacgc tttggaaact gtgagacttg
      121 agtgcagaag gggagagtgg aattccatgt gtagcggtga aatgcgtaga tatatggagg
      181 aacaccggtg gcgaaagcgg ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004264             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004264
VERSION     KHUT01004264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtgatg
       61 taagttgggt gtttaaacct agggctcaac cttgggtcgc atccaaaact gcatgacttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004265             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004265
VERSION     KHUT01004265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           83 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ttgggcgtaa agggagtgta ggcggctagg
       61 aaagtcaggc gtcaaatgga agcgctcaac gctttcaatc gtctgatact tcttggctcg
      121 agtagtggag aggagatcgg aattcacggt gtagcagtga aatgcgtaga tatcgtgagg
      181 aagaccaaag gcgaaagcag atctctggaa acttactgac gctgaggctc gaaggccaag
      241 ggagcaaacg gg
//
LOCUS       KHUT01004266             251 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004266
VERSION     KHUT01004266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>251
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           92 g           44 t
ORIGIN      
        1 aacggggggg gcaagtgttc ttcggaatga ctgggcgtaa agggcacgta ggcggtgaat
       61 cgggttgaaa gtgaaagtcg ccaaaaagtg gcggaatgct ttcgaaacca attcacttga
      121 gtgagacaga ggagagtgga atttcgtgtg gaggggtgaa atctacagat ctacgaagga
      181 acgccaaaag cgaaggcagc tctctgggtc cctaccgacg ctggggtgcg aaagcatggg
      241 gagcgaacgg g
//
LOCUS       KHUT01004267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004267
VERSION     KHUT01004267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           85 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgagg
       61 caagtcaggt gtgaaatccc agggctcaac cctggaactg cacttgatac tactttgctt
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004268
VERSION     KHUT01004268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           87 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcac
       61 taagtcagca gtgaaatacg gcagctcaac tgtcgaggtg ctgttgatac tggtggactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag agatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004269
VERSION     KHUT01004269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          103 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggctcgt
       61 gaagtcgggt gtgaaagccc ggggctcaac cccggaacgg catccgatac tggcgggctt
      121 gagaccggga gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004270             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004270
VERSION     KHUT01004270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttaga
       61 caagtcgggt gtgaaagctc gaggctcaac ctcgagacgc cactcgatac tgttttgact
      121 ggagtccggt agaggagtgc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004271
VERSION     KHUT01004271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           87 g           47 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgctg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cattcaaaac tacagtgctt
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacccgt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004272
VERSION     KHUT01004272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           92 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggttcga
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaagac tgtcgagcta
      121 gagtgtggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttactgga ccacgactga cgctgcaact cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004273             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004273
VERSION     KHUT01004273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggttggg
       61 taagtctgtc tgtgaaagcc cggggctcaa ccccgggact gcaggggata ctgctcgact
      121 ggagacaagc agaggttggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggccaactgg gcttgttctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004274
VERSION     KHUT01004274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          100 g           40 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggtgggg
       61 caagtgccgt gtgaaagccg gtggcttaac tgccggaggc catggcagac tgcctgactt
      121 gagcccgaga gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggagaaatca gcggactggc tcggtgctga cactgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004275
VERSION     KHUT01004275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           94 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggtggtgagg
       61 taagatgcat gtgaaatccc tgggcttaac ctaggaatgg catggatgac tgtctcgcta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga caaatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004276
VERSION     KHUT01004276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           89 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggattg
       61 caagtcagat gtgaaattct tgagctcaac tcaagcgctg catctgaaac tgtgattctt
      121 gagtgctgga gaggaaagcg gtattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004277
VERSION     KHUT01004277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           86 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaactg cattgaatac tatctggcta
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004278
VERSION     KHUT01004278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          107 g           42 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcgggaggc
       61 caagtctgcg gtgaaatcgc tcggctcaac tgggcggggt ccgtggaaac tggtcacctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004279             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004279
VERSION     KHUT01004279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtcttg
       61 caagctgggt gtgaaagccc cgggctcaac ctgggaggtg cattcaggac tgtgaggctt
      121 ggagtgcggc agagggaggt ggaattccac gtgtagcagt gaaatgcgta gagatgtgga
      181 ggaacaccga tggcgaaggc agcctcctgg gccgacactg acgctcatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004280
VERSION     KHUT01004280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcatgta ggcggttcgt
       61 caagtcgtgt gttaaagccc ctggctcaac tgggggaggt catgcgagac tgccggactt
      121 gaggacagta gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg acgtgctggg ctgtaactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004281
VERSION     KHUT01004281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcccga
       61 taagtctgct gtgaaagtcc agggctcaac cctggaatgc cagtggatac tgtcgggcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004282
VERSION     KHUT01004282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           79 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggctata
       61 taagtcagtg gtgaaagccc gcggctcaac cgtggaattg ccattgaaac tgcatagctt
      121 gagtacagtt gaagtgggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gctcactaag ctgtaactga cactgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004283
VERSION     KHUT01004283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtaat
       61 caagtcaggt gtgaaatccc tgggctcaac ccaggaactg cacttgaaac tggttgcctt
      121 gagtgttgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga caattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004284
VERSION     KHUT01004284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacggagggg gccagcgttg ctcggaatta ctgggcgtaa agggcttgta ggcggtctga
       61 taagtcctgt gtgaaatccc ctggcttaac cagggaactg cgccggatac tgtcggacta
      121 gaggccggga gagggaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgtaggcg gcttcctgga ccggttctga cgctgaggag cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01004285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004285
VERSION     KHUT01004285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           88 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccttc
       61 caagtcaggt gtgaaatccc acagctcaac tgtggaacgg cgcttgaaac tggttggctt
      121 gagtcagcta gaggtacacg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaagcg gtgtactgga gctggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004286
VERSION     KHUT01004286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           80 g           53 t
ORIGIN      
        1 tacgaaggtt gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 taagtctatt gtgaaagctc ccggcttaac tgggagaggc caatggatac taccaagctt
      121 gagtatagga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaagca ggtaactgga ctattactga cactcaactg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004287
VERSION     KHUT01004287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ttgggcgtaa agcgcgcgca ggcggtctct
       61 taagtctgat gtgaaatctc gcggctcaac cgcgagcggc cattggaaac tgggagactt
      121 gagtgcagaa gagaagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctttggt ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004288
VERSION     KHUT01004288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtcaga
       61 tcagtctgga gtgacagtcg tcggcttaac cgacgaagtg ttctggaaac tgtctgactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgaaggca gtgtcctggg aagtacctga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004289
VERSION     KHUT01004289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttgcg
       61 caagacaggt gtgaaatccc agggctcaac cttggaattg cacttgtgac tgtgcggctg
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca gtcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004290
VERSION     KHUT01004290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggtcctg
       61 caagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tgcagggcta
      121 gagtgtggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgcagacg gctttctgga ccataactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004291             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004291
VERSION     KHUT01004291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c           99 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa aacgcttgta ggtggccacg
       61 ccaagtcgga tgtgaaatct cccggcttaa ctgggagggg tcattcgata ctggcgggct
      121 cgaggcaggt agggggaagc ggaattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggcttcctgg acctgttctg acactgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01004292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004292
VERSION     KHUT01004292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c          100 g           39 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggcacga
       61 tcagtgcggc gtgaaagtcc tgggctcaac ctggggacgg cgctgcagac tgtcgagctg
      121 gagtccggga gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggc ccggaactga cgctcaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004293
VERSION     KHUT01004293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgca ggcggtagtc
       61 caagtctgtt gtgaaatctc ccggcttaac cgggagggtg cggcggaaac tgggctgctg
      121 gagtatggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggtgcagacg gcttgctgga ccatcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004294
VERSION     KHUT01004294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccatg
       61 caggtccgct gtgaaaacta gaggctcaac ctctagaggt cggtggaaac cgcatggcta
      121 gagtccggaa gaggggactg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacacccgt ggcgaaggcg ggtccctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004295
VERSION     KHUT01004295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggggata
       61 caagtggtgt atgaaatctc ccagcttaac tgggataggt tatgccagac tgtatttcta
      121 gagggcgaga gaggggggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gccccctggc tcgtacctga cgctcagcca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004296
VERSION     KHUT01004296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          101 g           38 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccggt
       61 caagtcggat gtgaaaaccc ggggctcaac cccgggcctg catccgatac tgatcggctc
      121 gaggaaggta ggggagagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004297
VERSION     KHUT01004297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgcc
       61 taagtcagac gtgaaatccc aaggcttaac cttggaactg cgtctgatac tgggtggctt
      121 gagtccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004298
VERSION     KHUT01004298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcgggccgt
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tggcggcctt
      121 gagtacggga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004299
VERSION     KHUT01004299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtcctg
       61 taagtcagat gtgaaagccc tgggcttaac ctgggaactg catttgaaac tgcaaggcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccataactga cactgaggca cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004300
VERSION     KHUT01004300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c          100 g           34 t
ORIGIN      
        1 aacgtaggac gcgagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggctctg
       61 caagtctgat gtgaaagcgc acggctcaac cgagcgaggc cattggagac tacagagctg
      121 gagggcagta gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004301
VERSION     KHUT01004301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggaccat
       61 ttagtcaggg gtgaaatccc ggggctcaac ctcggaatgg cctttgatac tggtggtctt
      121 gaggacggga gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctgga ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004302
VERSION     KHUT01004302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttctc
       61 caagtcaggg gtgaaatcct gcggcttaac cgtagaagtg cctttgatac tggagatctc
      121 gagggaggta ggggtccgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004303
VERSION     KHUT01004303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatta ctgggcgtaa agggtctgta ggcggttatt
       61 taggtctgat gtgacatctc gcggcttaac tgcgagcgcg cattggaaac cggaagacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004304
VERSION     KHUT01004304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtgact
       61 caagtcagat gtgaaatccc cgggcttaac ctgggaattg cgtttgaaac tgtgtcgctt
      121 gagtttagca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctagaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004305
VERSION     KHUT01004305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           98 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcaagca ggcggcctgt
       61 taagtttgac gtgaaatctc ctggcttaac tgggaggggt cgttggaaac tggcaggctt
      121 gaggcgatga gaggggtatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gtaccctggc attggcctga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004306
VERSION     KHUT01004306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 tcagttggat gtgaaagccc agggctcaac cttggaactg cattcaatac tgtcaggcta
      121 gagtacggga gaggggggta gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccggt ggcgaaggcg gccccctgga tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004307
VERSION     KHUT01004307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           84 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtacgta ggcgggtgtc
       61 taagtcagtg gtgaaagcct acagctcaac tgtggaattg ccattgatac tggatgtctt
      121 gagtatagta gaggtgggca gaattcatgg tgtagcggtg aaatgcatag ataccatgag
      181 gaataccgat agggaagcca gctcactgga ctattactga cgctaaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004308             258 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004308
VERSION     KHUT01004308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>258
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c          102 g           41 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcagtg
       61 caagtacgga gtgaaatctc cctgctcaac ggggagggtg ctctgtaaac tgcacagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtaaataa cctgacgctg aggcgcgaag
      241 gctaggggag cgaacggg
//
LOCUS       KHUT01004309             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004309
VERSION     KHUT01004309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           61 c           98 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 gacgtcggcc actgaaagcc cccggctcaa ccggggaagt ggtgccgaaa cgccccgtct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtcaactg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004310
VERSION     KHUT01004310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           93 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggatgcc
       61 taagccagac gtgaaatccc cgagcttaac ttgggaatgg cgtttgggac tgggcatcta
      121 gagtgcggca gaggggggtg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccgat ggcgaaggca gccccctggg ccagcactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004311
VERSION     KHUT01004311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcggatgtg
       61 taagtctggt tttaaagact gcggctcaac cgcagggatg gactggatac tgtatatctt
      121 gacctctgga gaggaaaccg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccaat ggcgaaggca ggtttctgga cagaaggtga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004312
VERSION     KHUT01004312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           81 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggggata
       61 taagtcagtg gtgaaagccc gttgcttaac aacggaactg ccattgatac tgtatctctt
      121 gaattcgatc gaagtgggcg gaatgtgaca tgtagcggtg aaatgcttag atatgtcaca
      181 gaacaccgat agcgaaggca gctcactagg tctggattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004313
VERSION     KHUT01004313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          103 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccggc
       61 tgcgtcggat gtgaaagccc ggggctcaac cccggaacgg catccgatac gggctggctg
      121 gagaccggga gaggagggtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004314
VERSION     KHUT01004314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttgtg
       61 tgagtgtgat gtgaaagccc cgggctcaac ctgggaagtg catcgcaaac gacacgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004315
VERSION     KHUT01004315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          100 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggggcca
       61 caagtggtgt atgaaatcgc tcggcttaac cgggcgcggt tatgccagac tgtggttctg
      121 gagtgcggga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc ccgacactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004316
VERSION     KHUT01004316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          104 g           44 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcttg
       61 taagtttgtg gtgaaagccc ggggctcaac cccgggtctg ccatggatac tgcgagacta
      121 gagtgctgta ggggctggta gaatgtcggg tgtagcggtg gaatgcgtag agatccgacg
      181 gaagaccggt ggcgaaggcg gccagctggg cagcaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004317
VERSION     KHUT01004317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtctga
       61 aaagtcttct gtgaaatcta cgggcttacc ctgtaacgtg cagaggaaac tgtcaggctt
      121 gagtatcgga gaggacaggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgataactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004318
VERSION     KHUT01004318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcagga gtgaaagccc taggctcaac ctaggaagtg cttttgatac tgcaaagctt
      121 gagtgtggga gaggatgctg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg ggcatctggc ccaacactga cactcagacc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01004319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004319
VERSION     KHUT01004319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatta ctgggcttaa agggtgcgta ggtgggcctg
       61 caagtgccct gtgaaatccc ccggctcaac cggggaactg cggggcatac tgcgggtctt
      121 gaggaaggta ggcgccgggg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg cccggctggg cctttcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004320
VERSION     KHUT01004320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           82 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcggactgt
       61 taagtcagtg gtgaaatccc ggggctcaac cccggaactg ccattgatac tatcagtctt
      121 gaatattgtg gaggtgagcg gaatgtgtca tgtagcggtg aaatgcatag agatgacaca
      181 gaacaccgat tgcgaaggca gctcactacg caatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004321
VERSION     KHUT01004321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           45 c           72 g           59 t
ORIGIN      
        1 tacagagacc tcaagtgtta tccggattta ttgggcgtaa agcgtccgca gattgtttga
       61 cgggttaggg gttaaaatcc agtgcttaac actggaactg cctttaaaac tatcaaacta
      121 gagggtggaa gaggtaagcg gaattgcagg tgtagtcgta ataagcgttg atatctgcaa
      181 gaacaccaaa ggcgaaggca gcttactagt acacttctga cgttcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004322
VERSION     KHUT01004322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggagtca ctgggcttaa agcgtacgta ggcgggcgcg
       61 caggcgtcgt gtgaaagccc tcagctcaac tgaggaactg cacggcgaac cgcgcgtctt
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaatgccgaa ggagaaatca ggccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004323
VERSION     KHUT01004323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           49 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggat gtgaaagctt agggctcaac cctgagaggc cattcgatac gggcaagcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01004324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004324
VERSION     KHUT01004324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggctgt
       61 caagtctggg gtgaaatccc gcggctcaac cgtggaactg cctcagatac tgatggcctc
      121 gagggaggta ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004325
VERSION     KHUT01004325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctgt
       61 taagtcggta gtgaaagccc tcggcttaac cgaggaagtg ctatcgaaac tggcagactt
      121 gagtaccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004326
VERSION     KHUT01004326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggacgat
       61 ttagtcagag gtgaaagccc ggggctcaac cccggaatag cctttgatac tggtcgtctt
      121 gagtttggga gaggtatgcg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcatactggc ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004327
VERSION     KHUT01004327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtagt
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tatctatctt
      121 gaattttgtt gaggtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgctaaa caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004328
VERSION     KHUT01004328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           59 c          108 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 ggagtcgggg gtgaaagtcc ggggctcaac cccggatcgg ccttcgatac tctcgcgctg
      121 gagcacagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004329
VERSION     KHUT01004329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          101 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcttca
       61 ggtgtcggat gtgaaagccc ggggctcaac cccggaggtg catccgaaac tctggagctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004330             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004330
VERSION     KHUT01004330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           37 c           79 g           62 t
ORIGIN      
        1 tacgaagggt gtgagcgttg cccggattta ttgggcgtaa agtgtttcgt aggcgttttg
       61 ataagtcatt ttttaaagct tcccgctcaa cgggaaaaaa gggaatgata ctatcgaaat
      121 tgaagaatgt tggagttact ggaactatcg gtgtaggggt gaaatccgtt gatatcgata
      181 ggaactccga aggcgaaggc gagtaactaa gcaaatcttg acgctgagga acgaaagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01004331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004331
VERSION     KHUT01004331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           86 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggtatt
       61 taagtcagag gtgaaagacg gccgctcaac ggtcgcagtg cctttgatac tggatatctt
      121 gagtgcagac gaggtctgtg gaatggatgg tgtagcggtg aaatgcttag ataccatcca
      181 gaacaccaat tgcgaaggca gcagactagg gtgccactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004332
VERSION     KHUT01004332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttctc
       61 taagtcaggt gtgaaatctc tcggctcaac cgggaaactg cgcctgatac tggaggactg
      121 gagtgaggca ggggtgtgtg gaacttccgg tggagcggtg aaatgtgtag atatcggaag
      181 gaacgccagc agcgaaagcg gcacactggg ccttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004333
VERSION     KHUT01004333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttct
       61 taagtctgct gtgaaagccc cgggctcaac ctgggaatgg cggtggatac tgagaagctg
      121 gagtacggta gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccttctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004334
VERSION     KHUT01004334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           99 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggctctg
       61 ttagtcaggc gtgaaagccc tgggctcaac ctggggactg cgcttgatac ggcagggctt
      121 gagggcagaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ctgtatctga cgctgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004335             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004335
VERSION     KHUT01004335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c           99 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtccggt gctgaaatcc cccggctcaa ccggggaagt ggcacggata cgaccggcct
      121 tgaggggggt aggggggtct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg acccttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004336
VERSION     KHUT01004336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtaaga
       61 tgtgtgtggt gtgaaagccc tgggctcaac ctaggaagtg catcgcaaac tgtcttgctg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004337
VERSION     KHUT01004337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           90 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggttggg
       61 taagtctgac gtgaaatctc cgggcctaac ccggaaactg cgtcggatac tatccagcta
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg agtctctgga cggttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004338
VERSION     KHUT01004338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           94 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggtgagta ggcggtttgt
       61 taagtcggtt gtgaaagccc ggggctcaac cccggagggt cgaccgatac tggcaggcta
      121 gagtacagaa gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggttactggg ctgatactga cgctgaatca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004339
VERSION     KHUT01004339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcccag
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tactaggctt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatactga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004340
VERSION     KHUT01004340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           98 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcatgg
       61 caagtcggga gtgaaaggcc tgggctcaac ccaggaactg ctttcgagac tgccaagcta
      121 gagtgcggaa ggggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccgga ggcgaaggcg ggttgctggg ccgacactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004341
VERSION     KHUT01004341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 atagtcggtg gtgaaatcct gcagcttaac tgtagaagtg cctccgatac tagcaagctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004342
VERSION     KHUT01004342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           95 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggccttc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaagac tggggggcta
      121 gagtgcggga tgggagagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctgt ggtgtagacg gctctctgga ccgcaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004343
VERSION     KHUT01004343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           92 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggtttgg
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgccaggcta
      121 gagtgtggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004344             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004344
VERSION     KHUT01004344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 aaagtcaggt gtgaaaactc caggctcaac ctggagacgc catctgatac ttctatggct
      121 tgagtacggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgatctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004345             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004345
VERSION     KHUT01004345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          105 g           41 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggaccgt
       61 caagcctggg gtgaaaggtc ggggctcaac cccgtacagg gccctgggga ctggcggtct
      121 ggaggcaggc agaggagcgt agaattcctg gtgtagcggt ggaatgcgta gagatcagga
      181 agaataccag tggcgaaggc ggcgctctgg gcctgtactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004346             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004346
VERSION     KHUT01004346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           36 c           79 g           67 t
ORIGIN      
        1 tacagaggtt acaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggcggattt
       61 ttaagttatt cttcaaagac tacggcttaa ccggaggaag gggggtaata ctggaagtct
      121 tgatttttag tggggtatct ggaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaactccga gggcgaaggc aggatactaa ctattatatg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01004347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004347
VERSION     KHUT01004347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctga
       61 caagtggaag gtgaaatccc tcggcttaac cgaggaactg ccttccaaac tgtttggctt
      121 gagtccggga gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcgctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004348
VERSION     KHUT01004348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           65 c          104 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agcgcccgca ggcggccaac
       61 caagccgcgc gtgaaatccc tcggctcaac tgggggcggt cgcgcgggac tggtcggctc
      121 gaggccggta ggggggagcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctccctgga ccggtcctga cgctcagggg cgaaagcgtg
      241 ggtagcgatc cgg
//
LOCUS       KHUT01004349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004349
VERSION     KHUT01004349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggcggccaag
       61 tgtgtcgggt gtgaaagctc gaggctcaac ctcgaaactg cgcccgaaac tatttggcta
      121 gagtattgga gagggaagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaat ggcgaaggca acttcctgga caattactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004350
VERSION     KHUT01004350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccatg
       61 caagtcgggt gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tacgtggctg
      121 gagttctgga gaggaaggtg gaatgctcgg tgtagaggtg aaattcgtag atatcgagtg
      181 gaacaccggt ggcgaaggcg gccttctgga cagaaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004351
VERSION     KHUT01004351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           81 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggatttt
       61 caagtcagag gtgaaagctt cccgctcaac gggaaaatgg cctttgatac tgttaatctt
      121 gagtacagtt gaggtgggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004352             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004352
VERSION     KHUT01004352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           97 g           43 t
ORIGIN      
        1 aacgtaggac gcaaacgtta tccggaatta ctgggcgtaa agggcgcgca ggcggtgggg
       61 taagtgaggc atgaaagctc cctggcttaa ccgggagagg atgtctgaga ctgcccgact
      121 agagtgaggt agaggggtgt agaattccgc gtgtagtggt gaaatgcgta gagatgcgga
      181 ggaataccag tggcgaaggc ggcaccctgg accttaactg acgctgaggc gcgaaagcat
      241 gggtagcgaa cggg
//
LOCUS       KHUT01004353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004353
VERSION     KHUT01004353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcttgta ggcggttcgt
       61 taagtctggt gtgaaatctc ccggctcaac cgggagcggc catcagatac tggcgagcta
      121 gaggtgggta gaggaaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga cctaccctga cgctgaggag cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004354
VERSION     KHUT01004354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           83 g           53 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggtttgt
       61 taagtttgtt gttaaagact ctggcctaac tggagaaaag caataaaaac tggcaaccta
      121 gagagtgaaa gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggcg gcttcttggt tcacatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004355
VERSION     KHUT01004355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacgaggggg gctagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggtttt
       61 taagttaggt gtttaatccc ggggcccaac cccggaccgg cacttaaaac tgggaatctt
      121 gaatagtgga gaggtcagtg gaattagagg tgtagcggtg gaatgcgtag agatctctaa
      181 gaacaccagt tgcgaaggcg gctgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004356
VERSION     KHUT01004356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           76 g           62 t
ORIGIN      
        1 tacgtaggtc ccaagcgtta tccggaatta ctgggcgtaa agagtctgta gtcggtctta
       61 aaagtctact ttcaaatacc tcggcttaac cgaggaaagg gggtggatac tttaagtctg
      121 gaagttttca ggggtgagta gaattcccgg tggagaggtg aaattcgttg atatcgggag
      181 gaatgccaaa agcgaaagca gctcactatg aatatcttga cgatgagaga cgaaagtttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004357
VERSION     KHUT01004357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           86 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgtgt
       61 taagtgtgat gttaaatcgt ggggctcaac cctgtcggtg catcacatac tggcatgcta
      121 gaggacagca gaggcaagtg gaattggtag tgtagcagtg aaatgcgtag atattaccag
      181 gaacaccaat ggcgaaggca gcttgctggg ctactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004358
VERSION     KHUT01004358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           37 c           84 g           59 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtttgta ggcggttata
       61 caagtcagtg gtgaaagccc agggcttaac cctggaattg ccattgaaac tgtatagcta
      121 gagagtgtta gaggttagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gctaactggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004359
VERSION     KHUT01004359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           88 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttccg
       61 caagtcagat gtgaaatccc cgggctcaac ctgggaactg catttgaaac tacggggcta
      121 gagtgtgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg atgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004360
VERSION     KHUT01004360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtaata
       61 caagtctggg gtgaaatccc tgagctcaac tcaggacgtg ccttggaaac tgtattacta
      121 gagtgctgga gaggtccgca gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccaga ggcgaaggcg gcggactgga cagtcactga cactcaacta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004361
VERSION     KHUT01004361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcctct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggggggctt
      121 gagtgcagaa gaggagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004362             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004362
VERSION     KHUT01004362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           95 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggttttc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgctagaaac tggaaggcta
      121 agagtgtggg agaggtaggc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacctg tggtgtagac ggcttactgg accatgactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004363
VERSION     KHUT01004363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           99 g           44 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggctgtc
       61 caagtcgggt gtgaaagccc cgggctcaac ccgggatgtg catccgagac tgggcggctc
      121 gagttcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactgga ccgacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004364
VERSION     KHUT01004364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           96 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtttgc
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaacag cgtttgagac tggcaggcta
      121 gaggttggaa gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ccacacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004365
VERSION     KHUT01004365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggtttcg
       61 caagtcaggt gtgaaagccc gaggctcaac ctcggaagtg cacttgatac tacggagctg
      121 gagtcttgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caagtactga cactgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004366
VERSION     KHUT01004366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc ttagcttaac tgaggaactg cgcccaaaac tactgtgcta
      121 gagggagaca ggggtaagcg gaacttacgg tggagcggtg aaatgtgttg atatcgtaag
      181 gaacaccggt ggcgaaggcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004367
VERSION     KHUT01004367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtacctt gtgaaatcct gcggctcaac cgcagaacgg ctgggtatac tggcggtctt
      121 gggccaccta ggggcagatg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaaa ggtgaaaaca gtctgctggg ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004368
VERSION     KHUT01004368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatact tcagcttaac tggagaagtg cccttgatac tggcgggctc
      121 gagggaggta ggggcatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004369
VERSION     KHUT01004369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           89 g           45 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcttt gtgaaatccc ccggctcaac cggggaactg cttggcatac tggcgatctt
      121 gagccatcta ggggcgactg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaaa ggtgaaaaca ggtcgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004370
VERSION     KHUT01004370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcggcg
       61 taagtcggat gtgaaatccc cgagctcaac ttgggaactg catccgatac tgcgtcgcta
      121 gagtcccgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg accatctgga cgggcactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004371
VERSION     KHUT01004371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgtgca ggcgggacgg
       61 cgagtccgat gtgaaagccc ggggcttaac cccggaagtg cattggaaac tgtcgttctt
      121 gagtgctgga gaggaagggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaataccagt ggcgaaggcg cccttctgga cggcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004372
VERSION     KHUT01004372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctccg
       61 aaagtcaggt gtgaaagccc cgggctcaac ctgggaagtg cacttgaaac ctcggagctt
      121 gaatgccgga gagggtagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctacctgga cggctattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004373
VERSION     KHUT01004373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggattta ctgggcgtaa agagcgcgta ggtggttcga
       61 taagtcggag gtgaaatccc tcggctcaac cgaggacccg cgtccgatac tgtcgaactt
      121 gagtgcagga gaggagagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gctctctgga ctgctactga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004374
VERSION     KHUT01004374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggtcgcg
       61 caagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tgcgaggcta
      121 gagtgcggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgtaactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004375
VERSION     KHUT01004375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 caagtctgat gtgaaatctc caggctcaac ctggaaaagg cattggaaac tgcttggctg
      121 gaggcttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004376
VERSION     KHUT01004376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggcggttcgt
       61 taagttggat gtgaaaactc tgggctcaac ccagagcctg catccaaaac tggcagacta
      121 gagttctgga gggggtagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagaaactga cgctcatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004377
VERSION     KHUT01004377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           38 c           88 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttg
       61 taagtaacat gtgaaagtcc tgagcttaac ttggggattg cgtgttaaac tgcagagcta
      121 gagtgttgta gaggatagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctatctgga caacaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004378
VERSION     KHUT01004378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttctg
       61 caagtctgat gtgaaatctc cgggcttaac ccggaaatgg cattggaaac tgcagggctg
      121 gagtgttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactactga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004379
VERSION     KHUT01004379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacggagggg gccagcgttg ctcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 taagtttgat gtgaaatccc atggctcaac catggaactg catctcatac tgccgagcta
      121 gagtctggga gagggaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccagcactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004380
VERSION     KHUT01004380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctgc
       61 caagtctgat gtgaaagccc tgggctcaac ccaggaagtg cgtcagatac tggtggactg
      121 gagtggcgga gaggatggcg gaatacccgg tgtagaggtg aaattcgtag atatcgggtg
      181 gaacacctgt ggcgaaggcg gccatctgga cgtcaactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004381             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004381
VERSION     KHUT01004381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           39 c           78 g           51 t
ORIGIN      
        1 tacgaggggg gcaagcgtta ttctaaatga ctgggcgtaa agggcacaca ggcggttttg
       61 caagttggat atgataaaaa acaaggctta accttgtaaa atcagccaaa actgcaaaac
      121 tcgagtaagt gagaggagaa tggaatttct ggaggagagg taaaattcgt agatatcagg
      181 aggaacgtcg aaagcgaagg caattctctg gcacttaact gacgctgatg tgcgaaagcg
      241 tggggagcaa aaggg
//
LOCUS       KHUT01004382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004382
VERSION     KHUT01004382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtaata
       61 cgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg cattgcaaac tgtgttactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004383             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004383
VERSION     KHUT01004383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           50 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ctgggcgtaa agagcgcgca ggcggcattt
       61 taagttgggc gtgaaatctc tcggcttaac tgagaggggt cgtccaaaac tggaaggcta
      121 acgagggcag gagaggaaag tggaattccc ggtgtagtgg tgaaatgcgt agatatcggg
      181 aggaacacct gtggcgaagg cgactttctg gcctgttcct gacgctgagg cgcgaaggct
      241 aggggagcga acggg
//
LOCUS       KHUT01004384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004384
VERSION     KHUT01004384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           94 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtttac
       61 caagtctgct gtgaaatctc ccggcttaac tgggagggtg cggtggaaac tggtaggctc
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcttactgga ccacgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004385
VERSION     KHUT01004385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           87 g           57 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggtggttaat
       61 taagtcgggt gtgaaatccc tgggctcaac cgaggaagtg catccgaaac taattagcta
      121 gagagtggca gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ccatttctga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004386
VERSION     KHUT01004386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           98 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtttgta ggtggcctgc
       61 taagtcggac gtgaaatccc tcagcttaac tggggaactg cgtctgagac tggcgggctt
      121 gagggccgga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga cggcatctga cactgagaaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004387
VERSION     KHUT01004387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           90 g           57 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttcac
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cattgaaaac tggtgggctt
      121 gagtatggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccatcactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004388
VERSION     KHUT01004388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           93 g           47 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtca
       61 taagtctgtg gtgaaatcct ccggctcaac cggagaactg cctcggatac tgtggctctc
      121 gagggtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccacttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004389
VERSION     KHUT01004389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggttcgta ggtggtccag
       61 caagtctgac gtgaaatccc atggctcaac cgtggaactg cgttggaaac tgcaggactt
      121 gagtaccgca gaggtgaagg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg cttcactggg cggttgctga cactgaggaa cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01004390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004390
VERSION     KHUT01004390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcgt
       61 tatgtcggat gtgaaagccc tcggcttaac cggggaagtg catccgaaac tggcgagctt
      121 gagtactaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctggg tagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004391
VERSION     KHUT01004391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           98 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgaat gtgaaaactc ggggctcaac cccgcgcttg cattcgatac gggcaggctc
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004392
VERSION     KHUT01004392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcgaac gtgaaatccc tcagcttaac tggggaactg cgtccgatac tgattggctt
      121 gaatccggaa gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctggg tcggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004393             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004393
VERSION     KHUT01004393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           90 a           36 c           67 g           62 t
ORIGIN      
        1 tacgaggggg gcaagcgtta ttcaaaatga ctgggcgtaa agggcatcta ggcggtttac
       61 tatgtttttt acaaaaaata ccagagctta actgtggtaa ttaaataaat aatattaaac
      121 tagagtaagt gcgaggaaaa tggaatttct ggagaagagg taaaatttgt agatatcaga
      181 aggaacatca aaagcaaagg caattttctg tcacttcact gacgctgacg tgcgaaagcg
      241 tggggagcaa ccggg
//
LOCUS       KHUT01004394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004394
VERSION     KHUT01004394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcactt gtgaaatctc cgggcttaac tcggaactgc caagtgatac tgtagtgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaggcg gattgctggg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004395
VERSION     KHUT01004395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtcggc
       61 caagtctgtt gtgaaatctc ccggctcaac tgggagggtg cgacggaaac tggtcggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgcagacg gctctctgga ccgcaactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004396
VERSION     KHUT01004396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcatcg
       61 taagtccgat gtgaaagccc tgggctcaac ccaggaagtg cattggatac tgcggagctt
      121 gaatctggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaagattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004397
VERSION     KHUT01004397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcgtgc
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tggtgcgctt
      121 gaatatacat gaggtaggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gcttactaag gtattattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004398
VERSION     KHUT01004398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           90 g           47 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggctgtt
       61 caagtctgct gtcaaagcgc agagcttaac tctgtacagg cggtggaaac tgaagagcta
      121 gagtatggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg ctctactggg ccataactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01004399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004399
VERSION     KHUT01004399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacggaggga gcaaacgttg ttcggattta ctgggcgtaa agggagtcta ggcggcttgg
       61 taagtcaggg gtgaaatccc gcggctcaac cgcggagttg cccttgatac tgccgagctt
      121 gaggccgtga gaggagactg gaattcccag tgtagcggtg aaatgcatag atattgggaa
      181 gaacaccagt ggcgaaggcg agtctctggc acggatctga cgctgaagct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004400
VERSION     KHUT01004400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           96 g           42 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtgagt
       61 taagtctgct gtgaaagccc cgggctcaac ctgggaatgg cagtggaaac tggctcgctg
      121 gagtatggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004401
VERSION     KHUT01004401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgccg
       61 taagtcggat gtgaaatctc ggggctcaac cctgaaactg cattcgatac tgcggtgcta
      121 gaggactcta gaggaaactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg ggtttctgga gagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004402
VERSION     KHUT01004402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcttgg
       61 taagttagag gtgaaagcct ggagctcaac tccagaattg cctttaagac tgccgagcta
      121 gcaggtgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcatactgga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004403
VERSION     KHUT01004403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctttc
       61 cacgtcggat gtgaaagctc ggggctcaac cctgagatgc cattcgatac gagaaagcta
      121 gaggtaggta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01004404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004404
VERSION     KHUT01004404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacgaaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggggtga
       61 aaggtacctt gtgaaatccc ttggctcaac taaggaattg cttggtaaac cggcactctt
      121 gaggcaggta gaggtgggcg gaactatggg tggagcggtg aaatgcgtag agatccatgg
      181 gaacaccggt ggcgaaagcg gcccactagg cctgaactga cgctgagacg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004405
VERSION     KHUT01004405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 aacgtagggg acgagcgtta tccggattta ctgggcgtaa agcgcatgta ggtggcgcgg
       61 aaagttggac gtgaaagctc ccggcttaac tgggagaggt cgttcaaaac ttccgcgctt
      121 gaggttggaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tcacacctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004406
VERSION     KHUT01004406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtgccg
       61 taagttgggt gtgaaatctc ggggcttaac cccgaaactg cattcaatac tgcggtgctc
      121 gagtactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaggcg ggtctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004407
VERSION     KHUT01004407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccaaa
       61 caagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgcgtggctt
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004408             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004408
VERSION     KHUT01004408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcgggt gtgaaatctc caggctcaac ctggaggcgc cactcgatac tgtcgtgact
      121 tgagttcggt agaggagtat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacacccg tagcgaaggc ggtactctgg gccgaaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004409
VERSION     KHUT01004409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggcttac
       61 caagtctggt gtgaaatccc ctcgctcaac gagggaactg cgccggaaac tggcaagctc
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004410
VERSION     KHUT01004410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaactg cgctccaaac tgccgtgctt
      121 gaggaagcta gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004411
VERSION     KHUT01004411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccgaattta ctgggcgtaa agcgtgtgca ggtggctggg
       61 taagttggat gtgaaatctc ctggctcaac tgggagaggt cgttcaaaac tgccgagctc
      121 gagggcggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacacctgt ggcgaaagcg gcctcctgga ccgtacctga cgctaagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004412
VERSION     KHUT01004412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           77 g           56 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggcctaa
       61 taagtcagtg gtgaaatctc ctcgcttaac gaggaaactg ccattgatac tgttgggctt
      121 gagtacagat gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004413
VERSION     KHUT01004413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           46 c           74 g           58 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaatag
       61 taagtcagtg gtgaaagcct gttgcttaac aacagaactg ccattgatac tactaatctt
      121 gagtacatat gatgtgggcg gaatgtgcca tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgttactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004414
VERSION     KHUT01004414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 tacgtaggtc gcgaacgttg ttcggaatta ctgggcttaa agggcgcgta ggcggtttcg
       61 taagtcaagg ctgaaatccg gcggctcacc cgtcgacgtg gcctcgatac tgcggaacta
      121 gaatcatgga ggggtgcgcg gaacttgtgg tggagcggtg aaatgctcag atatcacagg
      181 gaacaccgga ggcgaaggcg gcgcactggc catgtattga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004415
VERSION     KHUT01004415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           76 g           60 t
ORIGIN      
        1 gacgaaggat tcaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggaaata
       61 taagtcagtg gtgaaatccc gcagctcaac tgcggaactg ccattgaaac tgcatttctt
      121 gaatacggtt gaggtagatg gaatgtaaca tgtagcggtg aaatgcttag atatgttaca
      181 gaactccgat tgcgaaggca gtctactaag ccgttattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004416
VERSION     KHUT01004416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           99 g           46 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcga
       61 gaagtcgagt gtgaaagccc cgggctcaac ctgggaattg cgctcgatac tcttgtgctt
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004417
VERSION     KHUT01004417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtcggac gtgaaaggcg cgggctcaac ccgcgaaggc cgttcgaaac tgccgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gctctctggt cgacctctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004418             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004418
VERSION     KHUT01004418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggcggcttgg
       61 caagtcgagg gtgaaatccc agagctcaac tctggaattg ccttcgagac tgctaggctt
      121 cgagtacggg agaggtgagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggctcactgg cccgttactg acgctcaggc gcgacagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01004419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004419
VERSION     KHUT01004419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          101 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtctgga gtgaaagccc gcggctcaac cgcggaagcg cttcggatac tgggggcctc
      121 gaggggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004420
VERSION     KHUT01004420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttac
       61 caagtcaggg gtgaaatttt gcggcttaac cgtaacagtg cctttgatac tggtaggcta
      121 gagggagaca ggggtctgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004421             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004421
VERSION     KHUT01004421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           44 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcaggt gtgaaattcc agggctcaac cctggaacgc cacctgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004422             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004422
VERSION     KHUT01004422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          104 g           38 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtttt
       61 gtaagtcggg agtgaaagac cggggctcaa ccccggagcg gcttccgaga ctgcaaacct
      121 ggagaccggt aggggcaggc ggaattcccg gtgtagcggt ggaatgcgta gatatcggga
      181 agaacaccgg tggcgaaggc ggcctgctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004423
VERSION     KHUT01004423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           88 g           57 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtctgat gttaaagact ggggctcaac ctcggaaatg cattggatac tggcagactc
      121 gagtgcagta gaggcaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgcaagcg acttgctggg ctgtaactga cgctgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01004424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004424
VERSION     KHUT01004424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggctttg
       61 caagtctgat gtgaaatctc cgggcccaac ccggaaatgg cattagaaac tgtgaggctg
      121 gaggcttgga gggggaactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg agttcctgga caagacctga cgctgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004425
VERSION     KHUT01004425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagtttgtc gtgaaatctc ccggcttaac tgggagggtg cggcgaaaac tggagtgcta
      121 gagggtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gctttctgga ccacttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004426
VERSION     KHUT01004426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctacc
       61 taagtcggat gtgaaagccc ctggcttaac cggggaagtg cacccgaaac tgggtagcta
      121 gagtgcaaaa gagggtcgcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg ttgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004427
VERSION     KHUT01004427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          105 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gaagtccgtg gtgaaagccc gaggctcaac ttcgggtcgg ccatggatac tcctgggctc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004428
VERSION     KHUT01004428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           83 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgcgt
       61 taagtgagat gttaaatcgt gtggctcaac catatcgatg catctcatac tggcgtgcta
      121 gaggacagca gaggcaagtg gaactcgtag tgtagcagtg aaatgcgtag atattacgaa
      181 gaacaccaat ggcgaaggca gcttgctggg ctactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004429
VERSION     KHUT01004429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           73 g           60 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggactga
       61 taagtcagtg gtgaaatctc tcagcttaac tgagaaactg ccattgatac tgttagtcta
      121 gagtatagat gacgttggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaaa ctataactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004430
VERSION     KHUT01004430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           98 g           40 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgct gtgaaagtcc agggctcaac cctggaaagc cggtggatac tgccgggcta
      121 gagtccggga gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctaa ggcgaaggca gctcgctgga acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004431
VERSION     KHUT01004431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           89 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttga
       61 caagccagat gtgaaatccc cgggcttaac ctgggaactg cgtttggaac tgtcaggcta
      121 gagtgtagca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004432
VERSION     KHUT01004432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcagct gtgaaagccc ggggctcaac cccggagggt cagctgatac tggcagacta
      121 gagtacagaa gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggttactggg ctgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004433
VERSION     KHUT01004433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          103 g           45 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtggg
       61 taagtccgct gtgaaagccc ggagcttaac tccggagggt cggtggatac tgcccatctt
      121 gaaggtgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctgga gcatacttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004434
VERSION     KHUT01004434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          107 g           38 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggggtcg
       61 gaagtcgggt gtgaaagccc cgggctcaac ccgggaacgg cgctcgagac tccggctctt
      121 gagtcccgga gaggcgggtg gaattcctgg tgtagcggtg aaatgtgtag agatcaggag
      181 gaacaccggt ggcgtaggcg gcccgctgga cggagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004435             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004435
VERSION     KHUT01004435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           51 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcagat gtgaaaactc agggctcaac ccggagacgc catctgatac tgctgtggct
      121 tgagtctggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgatctgg gccagtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004436
VERSION     KHUT01004436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c          103 g           40 t
ORIGIN      
        1 gacggaaggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggctccg
       61 taagtctggt gtgaaagccc tgggctcagc ccgggaaggg cgctagaaac tggggagctg
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004437
VERSION     KHUT01004437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           92 g           42 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtttcg
       61 cacgtccggt gtgaaagccc agggctcaac cctggaagtg cactggatac gacggagctg
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004438
VERSION     KHUT01004438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtcgtt
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catacaatac tggacgactg
      121 gagtgtggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004439
VERSION     KHUT01004439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggctcga
       61 taagttggac gtgaaaactc cgggcttaac ttggagaggc cgttcaatac tgtcgggctg
      121 gagggtgtca gagggaagta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaatacccgt ggcgcaggcg gcttcctggg gcacacctga cactgagaag cgacagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004440
VERSION     KHUT01004440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctaa
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tgctaggctt
      121 gaatatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcgacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004441
VERSION     KHUT01004441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           51 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgc
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgacaatctc
      121 gagtccgata gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactgga tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004442
VERSION     KHUT01004442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           93 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcccgc
       61 taagtctggt ttgaaagctg gtcgctcaac gatcagatgt ggctggaaac tggtgggctt
      121 gaatgcgatg gcggtcgccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaactccgat tgcgaaggca ggcgaccagg tcgtgattga cactgaggca cgagagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004443
VERSION     KHUT01004443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           86 g           57 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtgagat gtgaaagcgt gtggctcaac catatcgttg tgtttcatac tgacagacta
      121 gagggaagca gaggcaagtg gaattagcag tgtagcagtg aaatgcgtag agattgctag
      181 gaacaccaat ggcgaaggca gcttgctggg ctttttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004444
VERSION     KHUT01004444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 taagtcgagt gtgaaaaatc tgggctcaac ccagtggagc actcgatact gcggtgacta
      121 gagtacggta gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gcactctggg ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004445
VERSION     KHUT01004445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacgtaggat ccgagcgtta tccggaatta ctgggtgtaa agggtccgta ggcgggcccg
       61 tgcgtcgaag gttaaagccc acagctcaac tgtggaaatg ccctcgatac ggcgggtctt
      121 gagtgcgaga gaggaaggta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccagt agcgaaggcg gccttctggc tcgttactga cgctgaggga cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004446
VERSION     KHUT01004446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           56 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctatg
       61 taagtcgagt gtgaaagcct ggggctcaac cccagaagtg cattcgatac tgcgtagctt
      121 gagtggtaga gaggatagta gaattgttgg tgtagtggtg aaatacgtag atatcaacag
      181 gaataccggt ggcgaaggcg gctatctggc tacacactga cactcagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004447
VERSION     KHUT01004447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           94 g           41 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccaac
       61 taagtcagac gtgaaatccc caggctcaac ctgggaactg cgtctgatac tggatggctc
      121 gagttcggga gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004448
VERSION     KHUT01004448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           77 g           62 t
ORIGIN      
        1 tacgaaggat cctagcgttg tccggattta ctgggtttaa agggagcgta ggcggacttt
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaattg ccattgaaac tgattgtctc
      121 gagtgcggtt gaggtcactg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaggca ggtgactaag ccgtaactga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004449
VERSION     KHUT01004449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctgac
       61 caagtctggg gtgaaatcct gcagcttaac tgtagaagtg cctgggatac tggtcggctc
      121 gagggaggta ggggtctgtg gaactgctgg tggagcggtg aaatgcgttg atatcagcag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004450
VERSION     KHUT01004450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcggaccgt
       61 taagtgtctt gtgaaatccc acagctcaac tgtggaactg cttggcagac tggcggtctt
      121 gaggtgctca ggggcgagtg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaaa ggtgaaaaca gctcgctggg ggcatcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004451
VERSION     KHUT01004451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggttgc
       61 caagtctgtg gtgaaatcct ccggcttaac cggagaactg ccgtggatac tggcgacctc
      121 gaggggtgta ggggcttgcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaagcg gcgagctgga caccttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004452
VERSION     KHUT01004452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgttg
       61 caagtgagat gtgaaatccc tgggcttaac ctaggaaccg cattttagac tgcaatgcta
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004453
VERSION     KHUT01004453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttggt
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaactg cctttgatac tgactggctg
      121 gagggagata ggggttgcgg gaactaccag tggagcggtg aaatgcgttg atattggtag
      181 gaacgccggt ggcgaaagcg cgcaactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004454
VERSION     KHUT01004454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          107 g           35 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttct
       61 taagcgggcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgggaagctc
      121 gagcccacta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtggagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004455
VERSION     KHUT01004455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           64 c          103 g           33 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggacctg
       61 gacgtgccgt gtgacagccc accgctcacc ggtggaaggc cacggtacac gacaggtctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01004456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004456
VERSION     KHUT01004456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c          101 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttga
       61 taagtagggg gtgaaatcct gtggcttaac tgcagggctg cctcctagac tgtctggctt
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004457
VERSION     KHUT01004457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggttcag
       61 acagtcagat gtgaaattcc cgggcttaac ctgggggctg catttgatac gtctgggctt
      121 gagtgaggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt cctttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004458
VERSION     KHUT01004458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           95 g           52 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttgg
       61 taagtcttgt gtgaaatctt cgggctcaac tcgaagtctg catgagaaac tgccgggctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004459             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004459
VERSION     KHUT01004459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           96 g           43 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcgggt gtgaaacccc caggctcaac ctggggacgc cacccgatac tgctgtgact
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004460
VERSION     KHUT01004460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           98 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcttgg
       61 ttagtccgac gtgaaatgta ccggttcaac cggggcactg tggcggaaac tgccgagctt
      121 gaggccgagg gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg accttctatc tcggatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004461
VERSION     KHUT01004461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 tacggaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtacgta ggcggcgctg
       61 ttagtcgggc gtgaaagccc tcggcttaac cgaggaattg cgcccgaaac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 ggtagcgaag ggg
//
LOCUS       KHUT01004462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004462
VERSION     KHUT01004462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           95 g           49 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggtggttgtg
       61 taagttcggc gtgaaatttc tcggcttaac cgggagtggt cgtcggatac tgcacgacta
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cactgaggcg cgatagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004463
VERSION     KHUT01004463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggct gtgaaatccc ggagctcaac tccgggtctg cagtcgatac gggcaaactg
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004464
VERSION     KHUT01004464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtggat
       61 taagtcgggt gtgaaatctc cgggctcaac ccggagcgtg cgcccgaaac tgatctgcta
      121 gaggatagga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga ctattcctga cactgaatca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004465
VERSION     KHUT01004465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          105 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agagcgcgta ggtgggaggc
       61 caagtctgtg gtgaaagcgc ccggctcaac tgggcatggt ccatggaaac tggtcacctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004466
VERSION     KHUT01004466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           98 g           38 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcgagg gtgaaagccc ccagctcaac tggggaagtg cctccgatac tggtcgtctc
      121 gagggggata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004467
VERSION     KHUT01004467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcgg
       61 caagtcaggc gtgaaatccc caggctcaac ctgggaactg cgcttgaaac tgccggtctt
      121 gagtgtcgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004468
VERSION     KHUT01004468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcttag
       61 caagtgaggt gtgaaatccc ttggctcaac caaggaattg cgcttcaaac tgctaggctc
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccaga ggcgaaggcg gctcactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004469
VERSION     KHUT01004469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 aaagtcaggg gtgaaatctt tcggcttaac cggaaaatgg cccctgatac tgattgtctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004470
VERSION     KHUT01004470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtcaggg gtgaaatact tcggcctaac cggagaacgg cctctgatac tggtgacctc
      121 gaggggtgta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004471
VERSION     KHUT01004471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcgatg
       61 taagtcgggt gtgaaatccc tcagcttaac tgaggaagtg cactcgatac tgtgtcgctt
      121 gagtgttgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga caactactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004472
VERSION     KHUT01004472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           96 g           41 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcgggtgat
       61 caagtcgggc gtgaaagccc cgggctcaac ccgggaactg cgtccgaaac tggttgccta
      121 gagtgtcgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004473
VERSION     KHUT01004473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggtgacg
       61 tatgtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tacgtcgcta
      121 gagtgtcgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cgaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004474
VERSION     KHUT01004474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           82 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtggagcgg
       61 taagtcagtg gtgaaatact ccgactcaat cggagaactg ccgttgatac tatcgttctt
      121 gaatgtggtt gaggcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaataccaat tgcgaaggca gctcgctaag ccactattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004475
VERSION     KHUT01004475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 cacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtttgta ggcggccatg
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaatgg cgcccgatac tgcatggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcatctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004476
VERSION     KHUT01004476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c           97 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagtctagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcggactt
      121 gagggaggca gaggagaata gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gttctctggg ccttacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004477
VERSION     KHUT01004477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           57 c           88 g           44 t
ORIGIN      
        1 gacgaaggtg gcaaccgttg ctcggattca ctgggcataa agcgcgcgta ggtggctagg
       61 caggtcactg gtgaaatccc acggctcaac cgtggacccg ccagtgaaac cgccttgctt
      121 gaggacgaga ggggagaatg gaactcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg attctctgga tcgttcctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004478
VERSION     KHUT01004478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agagcacgta ggtggtctgc
       61 taagtcacat gtgaaatacc ccagctcaac cggggaactg cgtgtgatac tggcaggctc
      121 gaggtcggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004479
VERSION     KHUT01004479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttttt
       61 taagtcagag gtgaaagccc ggagctcaac tccggaattg cctttgagac tggaagactt
      121 gaacgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgttga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004480
VERSION     KHUT01004480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtttcc
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg cacttgaaac tgggaggcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004481
VERSION     KHUT01004481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           81 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggctgcgttg
       61 aaagtcagat gtgcaattca ggggctcaac ctctggactg catccgatac tacaatgcta
      121 gagtcatgga gggggatctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaac ggcgaaggca agatcctgga catgtactga cgctcatgca cgaaggctcg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004482
VERSION     KHUT01004482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           85 g           58 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagaacgta ggtggtttct
       61 taagtttggt cttaaagacc ggggctcaac cccgggaatg gatcgaatac tgggagacta
      121 gaggatgtta gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg gctttctggg acattcctga cactgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01004483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004483
VERSION     KHUT01004483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c          102 g           36 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcccgca ggcggtcgcg
       61 tcagttcggg gtgacagctc tcggcttaac cgagagagga ccttgaagac ggcgcgactt
      121 gagggccaga gagggacatg gaactccggg tggagtggtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tggcacctga cgctcggggg cgaaagcgag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004484
VERSION     KHUT01004484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          103 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggtggaagag
       61 gaagtctgct gtgaaagcgc ctggcttaac cgggcgaggt cggtggaaac tcctcatctg
      121 gaggtcggta gaggggggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccgcacctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004485
VERSION     KHUT01004485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          100 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtcctc
       61 tcagtttggg gttacagctg ccggctcaac cggcagagcg cctcgaagac gggaggactt
      121 gagggccaga gagggacaca gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggc tggtgactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004486
VERSION     KHUT01004486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtcaggg gtgaaatact ccggcctaac cggagcagtg cttttgatac tggtgacctc
      121 gaggggtgta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga catcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004487
VERSION     KHUT01004487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgta ggtgggtcgg
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccattgatac tatcgatctt
      121 gaattatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactacg gatatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004488
VERSION     KHUT01004488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggttcgg
       61 caagtttggg gtgacagtgc tcggcttcac tgagcaagga ctctgaaaac tgcgggactt
      121 gaggaccaga gagggacata gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggc tggtaactga cgctcatgtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004489
VERSION     KHUT01004489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggttcgg
       61 taagtcacca gtgaaatcta tgggctcaac ccatagtctg ctggcgaaac tgccgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004490
VERSION     KHUT01004490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtttggt gtgaaatctc cgagctcaac tcggaaacgg cactaaaaac tactcggctg
      121 gagggtcgga aggggggctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg agtccctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004491
VERSION     KHUT01004491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgctcgta ggtggatcgg
       61 taagtctgca aagaaagacc cgggctcaac tcggggaacg gtgtagatac taccggtctt
      121 gaggcaatca gaggatgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcatctggg gttgttctga cactgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004492
VERSION     KHUT01004492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           88 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggtggtgctc
       61 caagtctgtt gtgaaatctc ccggcttaac tgggagggtg cggcggaaac tggaatgctt
      121 gaggatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggtgtagaca gctttctgga ccatttctga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004493
VERSION     KHUT01004493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggaggtttgg
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacggaaac taccaggcta
      121 gagtgcggta gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca gtcttctgga ccgtcactga ctctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004494
VERSION     KHUT01004494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           75 g           63 t
ORIGIN      
        1 tacggagggt ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtcaga
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgtctgactt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactact aatatattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004495
VERSION     KHUT01004495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggggctc
       61 caagtggtgt atgaaatttc ccagcttaac tgggaagggt tatgccagac tggagttcta
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004496
VERSION     KHUT01004496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggtcccg
       61 caagtcaggc gtgaaatccc cgagcttaac ttgggaattg cgcttgaaac tatgggactc
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004497
VERSION     KHUT01004497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           84 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcaga
       61 taagtctgat gtgaaatctt cgagctcaac tcggaaactg caccggaaac tatctggcta
      121 gaggattgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004498
VERSION     KHUT01004498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcgaaatta ctgggcgtaa agggcgcgta ggcggtgata
       61 tgtgtgggat gtgaaagccc tgggcttaac ctaggaagtg cataccaaac tatattactg
      121 gagtagggga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004499
VERSION     KHUT01004499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttat
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cctctgaaac tggtagacta
      121 gaatactgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga cagttattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004500
VERSION     KHUT01004500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcattt
       61 tgagtttgat gtgaaagccc cgggcttaac ccgggaatgg cattgaatac tggagtgctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004501
VERSION     KHUT01004501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcgacc
       61 tgtgtcaggt gtgaaatccc cgggcttaac ctgggaattg cgcttgaaac taggttgctc
      121 gagttcagta gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg ctgaaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004502
VERSION     KHUT01004502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaatt
       61 caagtcagct gtgaaatctc tgggcttaac ccagaacggc cagctgatac tgctttgcta
      121 gagtgcagaa ggggcaatcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004503
VERSION     KHUT01004503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggaccat
       61 caagtcaggt gtgaaatccc tgggctcaac ctaggaactg catctgaaac tggcggtctt
      121 gagtgtcgga gagggaagcg gaattccggg tgtagaggtg aaattcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004504
VERSION     KHUT01004504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacgggggat gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggttttg
       61 tcggtcagat gtgaaagccc agggctcaac cctggacgtg catttgaaac agcaagactt
      121 gagtacggga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccgat ggcgaaggca gctttctgga ccgatactga cgctgaggcg cgaaggcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004505
VERSION     KHUT01004505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           54 c           80 g           50 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agggtccgca ggtggccgtt
       61 cacgtcacgg gtcaaatttc agggctcaac cctgaaaccg ctcgtgatac ggttcggctc
      121 gagcatggga gaggcaagcg gaattaccgg tgtagtagta aaatgcgtta atatcggtaa
      181 gaacaccaaa tgcgaaggca gcttgctgga acattgctga cactcaggga cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01004506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004506
VERSION     KHUT01004506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ctcggaatca ctgggcttaa agggtgcgta ggcggccctt
       61 caagtcgggt gtgaaagtct ccggcctaac cggagaattg cgcccgaaac tagagggctt
      121 gagtgcgcta ggggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcacactggg gcgcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004507
VERSION     KHUT01004507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           94 g           42 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatca ctgggcataa agagcacgta ggctgtcagt
       61 taagtctggc gtgaaagccc ccggctcaac cggggaactg caccggatac tggctgacta
      121 gaggacagga ggggtgagtg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactggc ctgtctctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004508
VERSION     KHUT01004508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           79 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcgggccta
       61 tcagtcagtg gtgaaatccc atagcttaac tatggaactg ccattgatac tgtaggtctt
      121 gaattcggtc gaagtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gctcactagg cctggattga cgctcatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004509
VERSION     KHUT01004509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           84 g           48 t
ORIGIN      
        1 tacggagact gcaagcgtta ctcggattca ctgggcgtaa agggtgcgca ggcggccagg
       61 tatgtcaggg gtgaaagccc acagctcaac tgtggaactg cctttgaaac tatctggcta
      121 gagtattgga gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccaaa ggcgaaggca gcttactgga caattactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004510
VERSION     KHUT01004510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggtggctggg
       61 tcagtcggat gtgaaagccc tgggcttaac ctgggaattg cattcgatac tacccggcta
      121 gagtatggga gagggaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ccaatactga cactcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004511
VERSION     KHUT01004511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c           98 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacgg ccagcttaac tggttgcttg ctttcgatac gggcagacta
      121 gagggatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattccctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004512
VERSION     KHUT01004512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          106 g           34 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgca ggcggtcctg
       61 tgcgtcggcg ctgaaagcgc ccggcttaac cgggcgaggt gcggcgatac gataggactg
      121 gaggctcgga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc cgagacctga cgctgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004513
VERSION     KHUT01004513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcgggg
       61 taagtgaggt gtgaaatccc agagctcaac tctggaactg cactttatac tgctctgctt
      121 gagtcttgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga caaagactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004514             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004514
VERSION     KHUT01004514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           87 g           56 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtgatt
       61 taagtttggt gtttaatccc ggggcttaac cccggttcgc atcgaaaact gggtcacttg
      121 agtgtaagag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggct tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004515
VERSION     KHUT01004515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatctt
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctttgatac tggggatctc
      121 gagtccggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004516
VERSION     KHUT01004516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggcgcg
       61 tcagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac tgcgcgcctg
      121 gagggggata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tccctactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004517             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004517
VERSION     KHUT01004517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcgat
       61 gaagtctgat gtttaatgcc ggggctcaac cccggtgcgc attggaaact ggtcgacttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004518
VERSION     KHUT01004518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agcgcgcgca ggcggtcagg
       61 taagtcggat gtgaaaggct acggctcaac cgtagagcgg catccgaaac tgcttgactt
      121 gagtgcagga gaggagagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actctctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004519             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004519
VERSION     KHUT01004519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           84 g           58 t
ORIGIN      
        1 tacggaagat gcgagcgtta tccggattta ttgggtttaa agggagcgta ggcgggctgt
       61 taagtcagcg gtcaaatgtc agggcccaac cttggcatgc cgttgatact ggcggccttg
      121 agttcacaca aggaaggtgg aattcgtcgt gtagcggtga aatgcttaga tatgacgaag
      181 aactccgatt gcgaaggcag ccttctgggg tgttactgac gctgaggctc gaaagtgcgg
      241 gaatcaaaca gg
//
LOCUS       KHUT01004520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004520
VERSION     KHUT01004520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          108 g           35 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcccct
       61 taagcgtgtg gtgaaagcgc ggggctcaac cccgcgtcgg ccatgcggac tggggggctg
      121 gagcagacca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtctggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004521
VERSION     KHUT01004521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c           98 g           45 t
ORIGIN      
        1 tacgtaggga cctagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgtc
       61 cacgtcggat gtgaaagctc ggggctcaac cccgagaggc cattcgatac gggacggctt
      121 gaggcagcta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg gctgatctga cgctgaggcg cgaaagctgg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004522             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004522
VERSION     KHUT01004522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           28 c           69 g           68 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agagcacgta ggtggtttgt
       61 tatgtgtact attaaattgt aaagcataac tttataatgt agtacagaca actaagctag
      121 agttaggtag agggtagtag aatttctaat gtaaaggtga aatttgataa aattagagag
      181 aataccaatg gcgtaggcaa ctacttgggc cttaactgac actgaggtgc gaaggtatgg
      241 ggagcaaata gg
//
LOCUS       KHUT01004523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004523
VERSION     KHUT01004523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           94 g           42 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaaccg ctgggcatac tggcggtctt
      121 gggccactca ggggtcactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaagaca ggtgactggg ggtgtgccga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004524
VERSION     KHUT01004524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           99 g           43 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcagcc
       61 taagtaaggt gtgaaatctc cctgcttaac ggggaggctg cactttagac tgggcagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004525
VERSION     KHUT01004525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           86 g           55 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggttttt
       61 taagtttgat gtgaaatccc ggggctcaac cctggaattg cattgaatac tgaagaacta
      121 gagatctgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagatactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004526
VERSION     KHUT01004526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          102 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctgtg
       61 taagtcacct gtgaaattcc ggggctcaac tccggggcgg caggtgagac tgcatagctg
      121 gagctcggta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg gctcgctgga ccgatgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004527
VERSION     KHUT01004527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           92 g           41 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggaccg
       61 caagtcagat gtgaaatccc cgggctcaac ccgggaactg catctgaaac tgcggacctt
      121 gagtactgga gagggcggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccgcctgga cagtcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004528
VERSION     KHUT01004528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggctgtgggg
       61 taagtctggt gtgaaatccc ggagctcaac tccggaactg cactggatac tgctttacta
      121 gagcactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagttgctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004529
VERSION     KHUT01004529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtttgat gtgaaatctc cgagcttaac tcggaaatgg cattgaatac tattcggcta
      121 gagagtcgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cgatttctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004530
VERSION     KHUT01004530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggc
       61 aaagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgaaac tagtcggctc
      121 gagtgagata ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acatgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004531
VERSION     KHUT01004531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccgag
       61 taggtctgac gtgaaatctc gaggctcaac ctcgaggggt cgttggaaac cacccggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004532             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004532
VERSION     KHUT01004532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           46 c           99 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggtttct
       61 taagtctggg gtgaaaggtt caacgctcaa cgtgaacagt gccttggata ctgggagact
      121 tgagtctggg agggggtagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggctacctgg cctaagactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004533
VERSION     KHUT01004533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttttg
       61 taagtcgggt gtgaaagccc caggcttaac ctgggaactg cattcgagac tgcatgacta
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg actgcctggc ccaatactga cactcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004534
VERSION     KHUT01004534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           99 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctgg
       61 caagtccgat gtgaaatccc gaggcttacc ctcggaagtg cattggatac tggcgggcta
      121 gagtcccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004535
VERSION     KHUT01004535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtcagct gtgaaaacct gcggcttaac cgtgggcgtg cagctgatac gggcaaacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004536
VERSION     KHUT01004536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacagagact gcgagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtcaggt gtgaaatctc gaggcttaac ctcgaaattg cgcctgaaac tacacggcta
      121 gagtactgga gagggaaacg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gtttcctgga cagttactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004537
VERSION     KHUT01004537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtttac
       61 caagtcgggg ttgaaatctt tcggcctaac cggaaaagtg gcttcgatac tggtagactg
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004538
VERSION     KHUT01004538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           94 g           51 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggttttt
       61 taagtgaggc gtgaaagccc tgggcttaac ccaggaggtg cgtttcatac tggaagactt
      121 gagtacgaga gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctggc tcgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004539
VERSION     KHUT01004539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           85 g           51 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggtgtgc
       61 taagtcggat gtgaaatctc acagcctaac tgtgataggt cattcgaaac tggcatgcta
      121 gaggattgga gaggagactg gaatagttgg tgtagcagtg aaatgcgtag agatcaacta
      181 gaacaccggt ggcgaaggcg agtctctgga caattcctga cgctcaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004540             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004540
VERSION     KHUT01004540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           42 c           79 g           56 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcctga
       61 caagttaaca gtggtaaatc gaggctcaac ctcgtggaag ctgataatac tgtttggctc
      121 gagtacagaa gaggttaacg gaactccatg tggagcggta aaatgtgtag atatatggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01004541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004541
VERSION     KHUT01004541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           84 g           49 t
ORIGIN      
        1 tacgtaagtt gcgaacgtta tccggaatca ctgggcgtaa agcgttcgta ggcggcatag
       61 caagtaggat ttgaaatccc ggggctcaac tccggagccg gatcctaaac tgttaagcta
      121 gaacagatca gagggcagag gaattcccgg tgtagcggta atatgcatag atatcgggag
      181 gaacaccagt ggcgaaggcg actgcctggg gtcttgttga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004542
VERSION     KHUT01004542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttagt
       61 taagctggat gtgaaagccc cgggctcaac ctgggaactg cattcagaac tggctggcta
      121 gagtacgaga gagggtagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacatcagt ggcgaaggcg actgcctggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004543
VERSION     KHUT01004543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtatga
       61 catgtgtgat gtgaaagccc cgggcttaac ctgggaagcg catcgcaaac tgtcgaacta
      121 gagtagatga gagggtgatg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gtcacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004544
VERSION     KHUT01004544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttatt
       61 taagttggac gtgaaatgcc tgggcttaac ctgggaactg cgtacaagac tggatagcta
      121 gagtgcggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctgttactga cactgaggca cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004545
VERSION     KHUT01004545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgcttatctt
      121 gagccactta ggggcagtcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaaa ggtgaaaaca gactgctggg agtgtgctga cgctgagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004546
VERSION     KHUT01004546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          106 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttctt
       61 caagcgtgtg gtgaaagtcc ggggctcaac cccggatcgg ccgtgcgaac tgggggactt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagg ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004547
VERSION     KHUT01004547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           86 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggtggccaag
       61 tgtgtcaggt gtgaaatccc ggggcttaac tccggaactg cgcctgaaac tgcatggcta
      121 gagcattgga gagggaagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg acttcctgga caattgctga cactcaggca cgaaagcgtg
      241 ggtagccaaa ggg
//
LOCUS       KHUT01004548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004548
VERSION     KHUT01004548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg cctcggatac tgctggtctt
      121 gagggaggta ggggtttgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgaactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004549
VERSION     KHUT01004549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtta ctgggcgtaa agggtccgca ggcgggctga
       61 taagtctgac gtgaaatctt ctggctcaac tagaaggggt cgttggaaac tatcggtcta
      121 gcatcccgca gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg cgcggatgga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004550
VERSION     KHUT01004550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggattgt
       61 taagtcgggg gtgaaatcct gaggctcaac ctcagaactg ccttcgatac tggcgatctt
      121 gagtccggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004551
VERSION     KHUT01004551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 gacgaggtgg acaaacgtta ttcggattta ctgggcttaa agcgtgcgta ggcggctcag
       61 aaagttgggt gtgaaagccc tcggctcaac cgaggaattg cgctcaaaac tcctgagctt
      121 gagtgagata ggggagagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctctctgga tcttttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004552
VERSION     KHUT01004552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 gacgaactgt gcaaacgtta ttcggaatca ctgggcttga agagtgcgta ggcggttttg
       61 taagtagggt gtgaaagcct ccggcttaac cggggaattg cgctctaaac tgcaaggctt
      121 gagtgaggta ggggtgtgtg gaacttctag tggagcggtg aaatgtgttg atattagaag
      181 gaacgccggt ggcgaaagcg acacactgga ccttgtctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004553
VERSION     KHUT01004553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtatg
       61 taagtcagtg gtgaaatccc cgagcttaac tcgggaactg ccattgatac tatatatctt
      121 gaatattctg gaggtatgcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacacctat tgcgaaggca gcatactacg gaaagattga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004554             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004554
VERSION     KHUT01004554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcaga
       61 ccaagtcgaa tgtgaaattc cagagcttaa ctctggacgg tcgttcgata ctggtcggct
      121 agagtgtgga agaggatgtc ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag cggcgaaggc gggcatctgg gctaacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004555
VERSION     KHUT01004555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtctgt
       61 cgcgtctggt gtgaaaactc ggggcttaac cccgggcctg catcgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccataactga cactgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004556
VERSION     KHUT01004556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c          100 g           40 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcgggaatg
       61 taagtgggtg gtgaaagcgc ccggctaaac cgggcgagga ccaatcagac tgcatttcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004557
VERSION     KHUT01004557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           48 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaagccc tcggctcaac cgaggaattg cgcccaatac taccgtgctt
      121 gagtgagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tcttttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004558             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004558
VERSION     KHUT01004558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           49 c           80 g           54 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggtta
       61 tacaagtcag aagtgaaatc tccgagctca actcggaatc tgtcttttga aactgtatga
      121 ctagaggggc aaagaggaag ctggaacgaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 tcggaacacc gatagcgaag gcaggcttct gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01004559             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004559
VERSION     KHUT01004559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           37 c           82 g           60 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtatga
       61 taagttagca gtggtaaatc gaggctcaac ctcgtggaag ctggtaatac tgttatgctt
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgtgtag atatatggaa
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01004560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004560
VERSION     KHUT01004560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggctagg
       61 aaagtcttct gtgaaatcta cgggctcagc ctgtaacgtg cagaggaaac tatctggcta
      121 gagtgtcgga gaggataggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004561
VERSION     KHUT01004561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           98 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcgtcg
       61 taagtcagat gtgaaagccc ttggcttaac caaggaattg catctgaaac tgcggtgctt
      121 gaatccggga ggggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggc ggcgaaggcg gctctctgga ccggtattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004562
VERSION     KHUT01004562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           97 g           52 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatta ttgggtgtaa agggtgtgta ggcggggttg
       61 taagtcaggg gtgaaaggca gcggcttaac cgttggaagg cctttgatac tgcagtcctt
      121 gagttccgga gaggccattg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacacctga agcgtaggcg aatggctgga cggcgactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004563
VERSION     KHUT01004563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgtgtgca ggcggactgg
       61 taagttggat gtgaaatctc ctggctcaac tgggagaggc cgtacaatac tgccggacta
      121 gagggcaaca gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctggg ttgtacctga cgctcagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004564
VERSION     KHUT01004564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagttggat gtgaaagctc ctggctcaac tgggagatgt cgttcaaaac tgccagacta
      121 gaggatggta gaggaaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccatctctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004565
VERSION     KHUT01004565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggttctg
       61 taagattggt gtgaaatctc ccggcttaac tgggagggtg cgccgataac tgcagggctt
      121 gagtattgga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctctctgga caatcactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004566
VERSION     KHUT01004566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggcgatt
       61 caagtcaggt gtgaaaggcc tgggctcaac ccgggaactg cacttgatac tgaattgctt
      121 gagtccggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004567
VERSION     KHUT01004567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          101 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcgggagat
       61 taagttgggt gtgaaagccc ggggctcaac cccgggatgc cgcccaagac tggtctcctt
      121 gaggacggaa gaggggagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccgtttctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004568
VERSION     KHUT01004568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtaa agcgcacgta ggtggtcgtg
       61 taagtggggt gtgaaatcgc gcggcttaac cgcgcgccgc cattccaaac ggcacgactc
      121 gagccacgca gagggtcctg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggggaagcca aggacctggg cgtgtgctga cactgaggtg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004569
VERSION     KHUT01004569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ctgagaatca ctgggtttaa agggtgcgta ggtggtccgt
       61 taagttcgtt gtgaaagccc tgggctcaac ccaggaactg cttcgaatac tggcggactt
      121 gaggctagta ggggttactg gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaacgccaat ggtgaagaca ggtaactggg ctagttctga cactgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004570
VERSION     KHUT01004570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           79 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggctgt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatcagtctt
      121 gaatattgtt gaggtgggcg gaatatgtca tgtagcggtg aaatgcatag agatgacata
      181 gaacaccgat tgcgaaggca gctcactaaa catgtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004571
VERSION     KHUT01004571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          107 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggctggg
       61 caagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgccgggctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004572
VERSION     KHUT01004572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgta ggcggtctgc
       61 taagtcaatt gtttaatctc ccggcctaac cgggaatcag cagtagatac tggcgaacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004573
VERSION     KHUT01004573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtgaga
       61 taagttagtt gtgaaaggcc tgggcttaac ctaggaactg catctaatac tgtctcgcta
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gccttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004574
VERSION     KHUT01004574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           93 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agcgaccgca ggcggtctga
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaactg cattcgaaac tggcagactt
      121 gagtacggga gaggggggtg gaattcctgg tgtaggggtg aaattcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg gccccctggc ccgcaactga cgctcatggt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004575
VERSION     KHUT01004575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           90 g           45 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcga
       61 aaagtcagat gtgaaatccc ggggctcaac cccggaattg catccgatac tctcgcgctc
      121 gaggactgga ggggagtctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactatt ggcgaaggcg agactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcgaag ggg
//
LOCUS       KHUT01004576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004576
VERSION     KHUT01004576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggatact
       61 taagtgggat gtgaaatacc cgggctcaac ccgggtgctg cattccaaac tgggtatcta
      121 gagtgtggga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actttctgga ccataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004577
VERSION     KHUT01004577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           99 g           43 t
ORIGIN      
        1 tacagaggtg tcaagcgttg ctcggtatca ctgggcttaa agggcgcgta ggctgtgcgg
       61 tacggttcgg gtgaaatccc tcggctcaac cgaggaactg cccgggcgac ggccgcgctg
      121 gactgcatta ggggacgatg gaactcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg atcgtctgga gtgcatgtga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004578
VERSION     KHUT01004578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           89 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggccga
       61 taagtgaggc gtgaaatccc tgagctcaac tcaggaactg cacctcaaac tgtcggcctt
      121 gagtggtgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004579
VERSION     KHUT01004579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcctaa
       61 taagtcagat gtgaaacccc cgggcttaac cctgggcttg catttgaaac tgttgggctt
      121 gaggacagga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004580
VERSION     KHUT01004580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggacctt
       61 taagtccggg gtgaaaaccc agagctcaac tctgggactg ccttggaaac tgggggtctt
      121 gagggcagga gaggacagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actgtctgga ctgcatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004581
VERSION     KHUT01004581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           76 g           57 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcgtcccta
       61 taagttacgg ttgaaatctc cttgctcaac gaggaaactg gccgtaaaac tgtagggatt
      121 gagtacactt gaagcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ttgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004582
VERSION     KHUT01004582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctag
       61 taagtcggtc gtgaaatccc taggctcaac ctaggaactg cgatcgaaac tgttaggcta
      121 gagtatcgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004583
VERSION     KHUT01004583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccgaattta ctgggcgtaa agggtgtgca ggcggttcga
       61 taagttgggt gtgaaatctc ccggctcaac tgggagagga cgctcaagac tgtcggactg
      121 gagggcggta gagggaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccgtacctga cgctgagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004584
VERSION     KHUT01004584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           99 g           44 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcacgta ggcggtgcgg
       61 taagttggac gtgaaagctc ccggcttaac tgggagaggt cggacaaaac tgccgtgctt
      121 gaggctgaga gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc tcagaactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004585             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004585
VERSION     KHUT01004585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           50 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttgag
       61 taagtcgggt gtgaaaactc tgggcttaac ccagagccgc cactcgatac tgctctgact
      121 tgagttcggt aggggagcag ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccgg tggcgaaggc gctgctctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004586
VERSION     KHUT01004586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggcgctg
       61 tgcgtggggt gtgaaagctc ttggcttaac tgagagaggc catcccatac ggcagtgctc
      121 gagtgatgca gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg cattggctga cgctcaagtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004587
VERSION     KHUT01004587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcctt
       61 taagtcagtg gtgaaatcct ggagcttaac tccagaactg ccattgatac tattggcctt
      121 gaattcagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004588
VERSION     KHUT01004588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgttcgta ggtggtcctt
       61 caagtctgct gtcaaagcgc agggcttaac tctgtaaagg cggtggaaac tgaagagcta
      121 gagtgcggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccagc agcgaaagcg ctctactggg cctgcactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01004589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004589
VERSION     KHUT01004589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacgtagggc gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttag
       61 taagtctact gtgaaagtcc agggctcaac cctggaatgt cggtggatac tgctgagctg
      121 gagtacggga ggggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctgga acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004590
VERSION     KHUT01004590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c           98 g           44 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggccatg
       61 taagtcggtt gtgaaatccc agggctcaac cctgggactg cgtccgatac tgcatggctc
      121 gaggcaggta ggggagagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgttctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004591
VERSION     KHUT01004591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          100 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggctcgt
       61 taagtcggtt gtgaaaaccc ggggctcaac cccgggcctg cgtccgatac tggcgagcta
      121 gaggcaggta ggggagagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004592
VERSION     KHUT01004592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 tacgtcggaa gtgaaaaccc ggggcttaac cctgggcctg ctttcgatac gggcggacta
      121 gagtatggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004593
VERSION     KHUT01004593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           42 c           77 g           55 t
ORIGIN      
        1 tacggagact gcgagcgtta ctcggattta ctgggcgtaa agggtgcgta ggtggttagg
       61 tgtgtcagat gtgaaaactt gaagctcaac ttcaagcctg cgtctgaaac tatctaacta
      121 gagtgttgga gaggtaagta gaattcctag tgtagcagtg aaatgcgtag atattaggag
      181 gaacaccaaa tgcgaaggca gcttactgga caacaactga cactaaagca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004594
VERSION     KHUT01004594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggacatt
       61 taagtcaggg gtgaaatccc agagctcaac tctggaactg cctttgatac tgggtgtctt
      121 gagtatgata gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactgga tcattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004595
VERSION     KHUT01004595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          111 g           34 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggatggg
       61 taagtcgggg gtgaaagacc ggggctcaac tccggggcgg cctccgagac tgctcgtctg
      121 gggaccggta ggggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004596
VERSION     KHUT01004596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          108 g           37 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcagt
       61 cgcatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgagat gggctgactg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctggctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004597
VERSION     KHUT01004597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgctcgca ggcggattaa
       61 taagtcgggg gtgaaatccc ggggctcaac ctcggaattg ccttcgatac tgttaatctc
      121 gagtccggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctcatgag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004598
VERSION     KHUT01004598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           91 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcga
       61 taagttgggg gtgaaagccc aaggctcaac cttggaattg ccttcgatac tgtcggtcta
      121 gagtccggaa gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004599
VERSION     KHUT01004599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtccat
       61 taagtcaggt gtgaaaggcc tgggctcaac ccaggaaatg cgcctgagac tggtggactt
      121 gagtccggga gaggatggtg gaactcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacccgt ggcgaaggcg gccatctgga ccggtactga cgctaatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004600             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004600
VERSION     KHUT01004600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           47 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggtggctcag
       61 caagtcgggt gtgaaattct caggctcaac ctgagaacgc cactcgatac tgctgtggct
      121 agagtccagt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcactctgg gctggtactg acactgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01004601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004601
VERSION     KHUT01004601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 taagtcttgt gtgaaatccc tcggcttaac tgaggaactg cacgagatac tgcgagactt
      121 gagatcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004602
VERSION     KHUT01004602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           47 c          105 g           56 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctggc
       61 tgcgctggtc atgtaatccc ggggcttaac tttggggttg tggctggtac gggttggctt
      121 gagtctggtt ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccagttctga cgctgaggag cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004603
VERSION     KHUT01004603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggaatg
       61 aaagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tttgtttctt
      121 gaatcttctg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctgactacg ggagcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004604
VERSION     KHUT01004604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           82 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggtcaaa
       61 tcagtcaggt gtgaaattct ggagctcaac ttcagaactg cacttgatac tattcgacta
      121 gagtcctaca gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaaggccggt ggcgaaagcg agtctctgga tagcgactga cgctcaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004605
VERSION     KHUT01004605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggccttg
       61 taagtcacgg gtgaaagccc tcggctcaac cgaggaactg cctttgatac tgcaaggctc
      121 gaggccggga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacccgt ggcgaaggcg gccacctgga ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004606
VERSION     KHUT01004606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          101 g           50 t
ORIGIN      
        1 tacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcttgt
       61 cgcgtctgcc gtgaaaggcc tgggcttaac ccgggttctg cggtggatac gggctggcta
      121 gaggtaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004607
VERSION     KHUT01004607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacggagacc tcgagcgtta ctcggattta ctgggcgtaa agggtccgta ggtggccaga
       61 tatgttgggt gtgaaatcct gcagcttaac tgtagaactg cgcccaaaac tatttggcta
      121 gagactcgga ggggtaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaac ggcgaaggca gcttactgga cgagatctga cactgaggga cgaaagtgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01004608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004608
VERSION     KHUT01004608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcccgca ggcggttcag
       61 caagtttcag gtgaaatctc ccggctcaac tgggagggtg cctggaaaac tgctggactt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004609
VERSION     KHUT01004609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           85 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggtggcgccg
       61 aaagttaggt gtgaaagttc gaggcttaac ctcgaaattg cacctaatac tccggcgctg
      121 gagtcccata gaggaatctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgaa
      181 gaaggcctgt ggcgaaagca agattctggg tggcgactga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004610
VERSION     KHUT01004610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcagag
       61 taagttggat gtgaaatcct ggggctcaac ctcagaattg cattcaatac tgctttgcta
      121 gggcgctgta gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg aatctctggg cagcagccga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004611             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004611
VERSION     KHUT01004611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcggat gtgaaaactc ggggctcaac cccgagacgc catccgatac tgctgtgact
      121 tgagtctggt aggggaacac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgttctgg gccagtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004612
VERSION     KHUT01004612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           86 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggcaatg
       61 taagtctgct gttaaagaat gaggctcaac ctcataccag cagtggaaac tacatagcta
      121 gagtgcgttc ggggtagagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaagcg ctctactagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01004613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004613
VERSION     KHUT01004613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           89 g           48 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agagtgcgta ggcggcttat
       61 caggcgggat gtgaaatccc tcggcccaac cgaggaattg cgttccgaac cgataggctt
      121 gaggtggata ggggcgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacacctgt ggcgtaagcg gctcgctggg tccattctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004614
VERSION     KHUT01004614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           94 g           44 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcgggttac
       61 caagtcatgt gtgaaagctc ccggctaaac tgggagagga cagatgatac tggtagtctt
      121 gagggggtta gaggtgtgtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gcacactagg accaacctga cgctgaggcg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004615
VERSION     KHUT01004615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           94 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcacgg
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgccgcgctg
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga caggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004616
VERSION     KHUT01004616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgcgc
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tggcggactg
      121 gagcgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccagc ggtgtagacg gctttctgga ccacagctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004617
VERSION     KHUT01004617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           90 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggaaggc
       61 taagtctggt ttgaaagcag gcggcttaac cgtctgatgt ggctggaaac tggctttctt
      121 gaatgtgttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggctactagg acatgattga cactgaggca cgagagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004618
VERSION     KHUT01004618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcccag
       61 caagtcggaa gtgaaatccc acggctcaac catggaagtg ctttcgaaac tgctgggctt
      121 gagtacaaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg ttgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004619
VERSION     KHUT01004619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tggcggtctt
      121 gggccactca ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gctcgctggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004620
VERSION     KHUT01004620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatga ctgggcgtaa agggcgtgta ggcggccaaa
       61 caagtctgga gtgaaagtcc tgcttttaag gtgggaattg ctttggaaac tgattggctt
      121 gagtgcggaa gaggtaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg acttactggg ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004621             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004621
VERSION     KHUT01004621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 caagccgggt gtgaaaactc caggctcaac ctggagacgc cacccgggac tgctatggct
      121 agagtccggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccggcactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004622
VERSION     KHUT01004622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtccgct gtgaaaaccc gaggctcaac ctcgggacgt cggcggaaac cctttggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004623
VERSION     KHUT01004623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           43 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgat
       61 caagtccgac gtgaaattcc tcggctcaac cgagggcctg cgtcggatac tgctcggctt
      121 gagtcccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga cgggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004624
VERSION     KHUT01004624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           92 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtcttc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggaaggctt
      121 gagtgtggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtaggca gcttgctgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004625
VERSION     KHUT01004625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           86 g           50 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggctga
       61 taagtcggat gtgaaatccc tgagctcaac tcaggaactg cattcgaaac tgtcagtctt
      121 gagtagtgga gaggaaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004626
VERSION     KHUT01004626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           92 g           47 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggttaac
       61 caagtcagat gtgaaagccc cgggcttaac ctgggaacgg catttgatac tggatgactg
      121 gagtacgaga gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccaga ggcgaaggcg gctttctggc tcgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004627
VERSION     KHUT01004627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           95 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctt
       61 caagtccggg gtgaaatcct ccagcctaac tggagaagag cctcggagac tgagggcctc
      121 gagggaggta ggggcaagcg gaactatggg tggagcggtg aaatgcattg atatccatag
      181 gaactccggt ggcgaaggcg gcttgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004628
VERSION     KHUT01004628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           86 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggtggatatt
       61 taagtgggat gtgaaatacc cgggcttaac ctgggtgctg cattccaaac tggatatcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cactgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004629
VERSION     KHUT01004629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           86 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttctg
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tacaggacta
      121 gagtgtggca gagggaggtg gaattccatg tgtagcagtg aaatgcgtag agatatggaa
      181 gaacatcgat ggcgaaggca acctcctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004630
VERSION     KHUT01004630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           83 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatcgg
       61 taagtcagtg gtgaaagccc cgagctcaac ttgggaattg ccattgatac tgccagtctt
      121 gagtacggtt gaagtgggcg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccgat tgcgtaggca gctcactaag ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004631
VERSION     KHUT01004631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           95 g           38 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccacg
       61 caagtcggat gtgaaatccc tcggctcaac cgaggaactg cacccgaaac tgcgaggctc
      121 gagtcctgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga caggtactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01004632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004632
VERSION     KHUT01004632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcgaacgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttcgt
       61 taagtgagat gtgaaagccc tgggcttaac ctgggaactg catttcagac tgacggactc
      121 gagtacagta gagggcggtg gaattccctg tgtagcggtg aaatgcgtag atatagggag
      181 gaacatcagt ggcgaaggcg gccgcctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004633
VERSION     KHUT01004633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattga ctgggcgtaa agggcgcgta ggcggttatt
       61 taagttggat gtgaaacccc tgggcttaac ctaggacgtg catacaagac tggataactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004634
VERSION     KHUT01004634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcggac
       61 ccagtcgggg gtgaaagctt cccgcccaac gggaaaaggg cctccgatac tggttcgctc
      121 gaggggggta ggggcatgcg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004635
VERSION     KHUT01004635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcccgg
       61 taagtaggag gtgaaatctt gcggctcaac cgcaaggctg ccttctaaac tgccaggctc
      121 gagcacagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ctgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004636
VERSION     KHUT01004636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggtggtctag
       61 caagtacttt gtgaaatgcc tgggctcaac ctgggacatg cttggtatac tgctggactt
      121 gaggcggata ggggtgcttg gaactctagg tggagcggtg aaatgcgtag atatctaggg
      181 gaacgccaga ggcgaaagcg gagcactggg tccgacctga cactgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01004637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004637
VERSION     KHUT01004637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           84 g           56 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggggtga
       61 taagtcagtg gtgaaatctt gcggcttaac cgtaaaattg ccattgatac tgtcattctt
      121 gagtaggctc gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agtgaagaca gctcacgagg gccaaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01004638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004638
VERSION     KHUT01004638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           87 g           51 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttgg
       61 taagttgggt gttaaatctc ttggctcaac caagaggtgt cgctcaagac tcccagactg
      121 gaggatggta gagggaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcctcctgga ccattcctga cactcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004639
VERSION     KHUT01004639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttacg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg catccgatac tgcgtggcta
      121 gagtttggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gcttcctggc ctaaaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004640
VERSION     KHUT01004640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaaag
       61 tatgtcgggt gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tacttagcta
      121 gagtcttgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga caatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004641
VERSION     KHUT01004641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttata
       61 caagcgagat gtgaaattcc cgggcttaac ctggggactg catttcgaac tgtatggcta
      121 gagttttgga gaggagagta gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaataccagt ggcgaaggcg gctctctggc caaaaactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004642
VERSION     KHUT01004642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacaggc gtgaaatccc cgggcttaac ctgggaactg cgtttgtgac tgcaaggcta
      121 gagtgtggca gaggggggta gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaataccgat ggcgaaggca gccccctggg tcaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004643
VERSION     KHUT01004643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agggcgtgta ggcggccctg
       61 taagtcggat gtgaaatccc gaagctcaac ttcggaactg cacccgatac tgcagggctt
      121 gaggtaggta gaggagagtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccaat agcgaaggca gctctctggg ccttacctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004644
VERSION     KHUT01004644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg cctccgatac tggcattctc
      121 gagtccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004645
VERSION     KHUT01004645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 aacgtaggag gcaaacgtta tccggattta ctgggcgtaa agcgcatgca ggcggttttt
       61 taagtcggac gtgaaagctc ctggctcaac taggagaggc cgttcgaaac tagaagactt
      121 gaggatggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccattcctga cgctcagatg cgaaagctag
      241 gggagagaac ggg
//
LOCUS       KHUT01004646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004646
VERSION     KHUT01004646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           84 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttatc
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaattg cattcgaaac tgaatagcta
      121 gagttcggca gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004647
VERSION     KHUT01004647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           90 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgctcgca ggcggtcttt
       61 taagtctgat gtgaaatctc gtggcttaac cacgaacggt cattggaaac tgggggactt
      121 gagtacagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004648
VERSION     KHUT01004648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           93 g           54 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttttc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggaaggcta
      121 gagtgcggga tgggtaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccgcaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004649
VERSION     KHUT01004649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           79 g           55 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcgtcccgg
       61 taagttatgg ctgaaatctc cccgctcaac ggggaaactg gccgtaaaac tgtcgggatt
      121 gagtacactt gaggcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgtaggca gctcgctaaa ttgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004650
VERSION     KHUT01004650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 tcagtctgtc gtgaaatcct ccagctcaac tggagaacgg cgatggatac tggtcgtctc
      121 gagggaggta ggggcgtgtg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg acacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004651
VERSION     KHUT01004651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           85 g           50 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatca ctgggcgtaa agcgtacgca ggcggatatg
       61 taagctagag gtgaaatccc ggggctcaac ttcggaactg cctttagaac tgcatatcta
      121 gagatcggaa gaggatggcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gccatctggt ccgatactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004652
VERSION     KHUT01004652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggggtc
       61 caagtggtgt atgaaatatc ccggcttaac cgggaagggt tatgccagac tggacttcta
      121 gagtgcgaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004653
VERSION     KHUT01004653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggtggccgga
       61 caagtcaggt gtgaaatccc tcagctcaac tgaggaactg cgcttgaaac tgtttggcta
      121 gagtgagcta ggggtgcttg gaactgccag tggagcggtg aaatgtgttg atattggcag
      181 gaacaccgga gccgaaaggg aagcactggg gcttaactga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004654
VERSION     KHUT01004654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtcttg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaactg cattcgagac tgcatgactg
      121 gagtatggga gagggcagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg actgcctggc ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004655
VERSION     KHUT01004655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 tacgtaggtc ccgagcgttg tccggaatta ctgggtataa agggtgcgca ggcggtcttg
       61 tgcgtcagaa gtgaaatatc acggctcaac cgtgagggtg cttttgatac ggcaagactt
      121 gagtacggga gaggatgatg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gtcatctggc ccgtaactga cgctcaggca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004656
VERSION     KHUT01004656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcatgta ggcggcttgt
       61 taagtcacgt gtgaaagccc caggctcaac ctgggaagtg cgcgtgaaac tggcgagctt
      121 gaatcctgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cagagattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004657
VERSION     KHUT01004657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           94 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcaag
       61 caagtcgaag gtgaaatccc acggctcaac cgtggaagtg cccccgatac tgcttgtctt
      121 gagggagata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004658
VERSION     KHUT01004658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggagggt
       61 gtagtcggtg gtgaaatcct ccagcttaac tggagaatgg cctccgatac tgactctctc
      121 gagggaggta ggggcatgtg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004659
VERSION     KHUT01004659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggacttt
       61 taagtcagtg gtgaaatcct gtagcttaac tgcagaactg ccattgatac tattagtctt
      121 gaatgtcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gctaactacg cgaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004660
VERSION     KHUT01004660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgac gtgaaatctc cgagcttaac tcggaaatgg cgttggatac tattcggctg
      121 gagggttgga agggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctgga caattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004661
VERSION     KHUT01004661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           92 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtccgca ggtggtcttt
       61 tacgtttggg gtgacagttc ccggctcaac tgggaaagtg cttcgaaaac ggggagactt
      121 gagggcttga gaggaaagcg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgtaggca gctttctggc aagtacctga cactcatgga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004662
VERSION     KHUT01004662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctt
       61 caagtcaagg gtgaaagcct ccggcttaac cggagaaggg cttttgatac tggagacctc
      121 gaggggtgca ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004663
VERSION     KHUT01004663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcatgg
       61 taagtcgggt gtgaaatctc agggctcaac cctgaaactg cactcgatac tgccgtgctc
      121 gagcactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccggt ggcgaaagct agtctctgga cagtcgctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004664
VERSION     KHUT01004664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaatccc gcagcttaac tgcggaatgg cattggatac tattcggcta
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004665
VERSION     KHUT01004665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtcta ggcggttgga
       61 taagtgggat gtgaaatgcc ccggctcacc cggggaccgg catcccaaac tgttcggctt
      121 gagtatggga gaggatgtgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cacatctggc ccaatactga cgctaaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004666
VERSION     KHUT01004666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagtcgggt gtgaaatccc ccagctcaac tggggaattg cgcctgaaac tgccgtgctc
      121 gaggaagaca gaggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcgtactggg tcttatctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004667
VERSION     KHUT01004667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 tacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatggg
       61 taagtacctt gtgaaatccc acagctcaac tgtggaactg cttggtatac tgcctgtctt
      121 gaggcactca ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaagaca gctcgctggg ggtgtactga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004668
VERSION     KHUT01004668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           83 g           51 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttttt
       61 aaagtcttct gtcaaagatc agggcttaac cctgagccgg cagaagaaac ttgaaggctt
      121 gagttcggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccaat ggcgaaagca ctctgctggg ccgtaactga cactgagaga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004669
VERSION     KHUT01004669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcggtg
       61 gaagtttgat gtgaaagctc agggcttaac cctgaaatgg cattgaatac tccattgctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004670
VERSION     KHUT01004670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttgtc
       61 caagtctggt gtgaaatctc ctggcttaac tgggagggtg cgctagaaac taggcaactt
      121 gagtacggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcttactgga ccgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004671
VERSION     KHUT01004671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c          102 g           33 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggccgca
       61 aacgtgcggt gtgaaagcgc ccggcttaac cgggcgaggc catcgcacac ggcgcggctc
      121 gagccgttca gaggtccctc gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaagacccac ggggaagcca ggggactggg aacgtgctga cgctcaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004672
VERSION     KHUT01004672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           81 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcaa
       61 taagtcagtg gtgaaatccg gcagctcaac tgtcgatgtg ccattgatac tgttggactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaga ctgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004673
VERSION     KHUT01004673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 taagttggat gtgaaatccc tgggcttaac ctgggaactg cattcaaaac tgcttgacta
      121 gagtttggta gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctacctgga ccagaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004674
VERSION     KHUT01004674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggaccag
       61 caagttaggg gtgaaatccc ggggctcaac cccggaactg cccttaaaac tgttgatctg
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004675
VERSION     KHUT01004675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           99 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccgt
       61 cgcgtcggga gtgaaaacct ggggctcaac cccaggcctg cttccgatac gggcggacta
      121 gaggtaggca ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004676
VERSION     KHUT01004676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcttac
       61 acagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgtttgatac ttgtaggcta
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004677
VERSION     KHUT01004677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgct
       61 taagtcagac gtgaaatccc tcggctcaac cggggcactg cgtctgagac tgaatggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004678
VERSION     KHUT01004678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttaag
       61 tatgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tgattaactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004679
VERSION     KHUT01004679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           89 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcttgc
       61 taagtttgat gtgaaatccc ggggctcaac cctggaattg cattgaatac tggcaggcta
      121 gagtactgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagtaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004680
VERSION     KHUT01004680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtccgat gttaaagacc ggggctcaac cccggcattg cgttggatac tggcaggctg
      121 gacgactgga gaggaaagta gaattaccag tgtagcggtg aaatgcgtag atactggtag
      181 gaatacccat tgcgaaggca gctttctgga cagtttgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004681
VERSION     KHUT01004681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           83 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggacttt
       61 taagtaggaa gtgaaaggtt ggagctcaac tccgacactg ctccctatac tggaagtctt
      121 gagttctgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccgat ggcgaaagca gctttctgga cagcaactga cgctgaggtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01004682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004682
VERSION     KHUT01004682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           89 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccaaa
       61 taagtcagtg gtgaaaccct tgggcttaac ccaaggagtg ccattgatac tgtgtggctt
      121 gggttatctc tgggcgggcg gaatggatgg tgtagcggtg aaatgcatag atatcaacca
      181 gaacgccgac agcgaaggca gctcgctggg gatataccga cgctgaggca cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004683
VERSION     KHUT01004683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgga gtgaaagctc ggggctcaac cccgaaaccg ctccgcatac tggcgagctg
      121 gagggatgca gaggtatgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcatactggg catcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004684
VERSION     KHUT01004684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgta
       61 caggtcagct gtgaaaactc gaggctcaac ctcgagacgt cggttgaaac cgtacggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004685
VERSION     KHUT01004685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggagcgt
       61 taagtcagtg gtgaaatccc gcagctcaac tgtggaattg ccattgatac tggcgctctt
      121 gagtgcggtt gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat cgcgaaggca gctcgcgaag ccgcaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01004686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004686
VERSION     KHUT01004686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           84 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgtt gtgaaagctc ccggctcaac tgggagaggc caacagaaac tatcaggctt
      121 gagtatggga gagggtactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaagca ggtacctgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004687
VERSION     KHUT01004687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggcggtttgc
       61 caagttgggg gtgtaagctc taggcctaac ctagaaattg cctccaaaac tagcatactt
      121 gagtattaga ggggaagctg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaggcg ggcttctggc taattactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004688
VERSION     KHUT01004688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           90 g           50 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggcggcttgg
       61 caagtcaggg gtgcaagctc taggcttaac ctagaaattg cctctgaaac tacctcgctt
      121 gagggtctga ggggaaactg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaggcg ggtttctggc agacttctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004689
VERSION     KHUT01004689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           88 g           48 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcag
       61 caagtcgggt gtgaaatccc ccagctcaac tggggaattg cgcccgaaac tgctgtgcta
      121 gaggaatata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tattctctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004690
VERSION     KHUT01004690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c          100 g           38 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctgcg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcgcggctt
      121 gagccttgta gagggcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgcctgga caagcgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004691
VERSION     KHUT01004691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c          101 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtgcgt
       61 taagtctgat gtgaaagccc tgggctcaac tcaggaattg cattggaaac tggcgtgctg
      121 gagtacggga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gtttcctggc ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004692
VERSION     KHUT01004692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgta ggctggcctg
       61 caagtcagat gtgaaatctc ggggctcaac cccgaaactg cgtctgaaac tacaggtcta
      121 gaatctcgga ggggaaaggg gtatttctca tgtaggggta aaatccgtag atatgagaag
      181 gaacaccaga ggcgaaggcg cctttctgga cgagtattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004693
VERSION     KHUT01004693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttatg
       61 aaagacaggc gtgaaatccc cgggcttaac ctgggaactg cgtttgtgac tacatgactc
      121 gagtacggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccagtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004694
VERSION     KHUT01004694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgttc aacggattca ttgggcttaa agcgtacgta ggcggatctg
       61 caggcgtcgt gtgaaagccc tcggctcaac cgaggaattg catggcgaac cgcaggtctt
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaatgccgaa ggtgaaagca ggccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004695
VERSION     KHUT01004695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           93 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggctttt
       61 caagtctagt gtgaaaaccc gaggcccaac ctcggaagtg cattggaaac tgaaaggcta
      121 gagtgccgga agggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg actctctgga cggtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004696
VERSION     KHUT01004696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           92 g           41 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaagcg
       61 taagtgcctt gtgaaatccc gcggcttaac cgcggaacgg ctgggcatac tgcgtttctt
      121 gagccagcca ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggcgaaagca gacgactggg gctgagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004697
VERSION     KHUT01004697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcctt
       61 caagtcaggg gtgaaagccc agagctcaac tctggaattg cccttgagac tgtaggactt
      121 gggttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgataccga cgctcaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004698
VERSION     KHUT01004698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg acaaacgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcaac
       61 caagtttggc gtgaaagctc ccggcttaac tgggagaggt cgttgaatac tggctggctt
      121 gaggcagtga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc attggcctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004699
VERSION     KHUT01004699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggacttt
       61 taagtgggat gtgaaatacc cgggcttaac ctgggtgctg cattccaaac tgggagtcta
      121 gagtgcagga ggggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004700
VERSION     KHUT01004700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcctgt
       61 cgcgtcgact gtgaaaaccc agggctcaac cctgggcctg cagtcgatac gggcaggcta
      121 gagtgcagta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ctgtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004701
VERSION     KHUT01004701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           42 c           97 g           56 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggaatta ctgggcttaa agggcgcgta ggcggtggcg
       61 taagtctgtg gtggaatttt atggcttaac cgtgaaattg ccatggaaac tgcgttactt
      121 gagtcggaca ggggaaggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaaggcctgt ggtgaagacg gccttctggg tcttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004702
VERSION     KHUT01004702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggtctgc
       61 taagcttgat gtgaaagccc cgggcttaac ctgggaacgg cattgaggac tggcagacta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004703
VERSION     KHUT01004703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatca ttgggcgtaa agagcgcgta ggtggcttgt
       61 caggtctgat gtgaaatcct ggggctcaac tccagaactg catcggaaac cggcttgcta
      121 gagtctggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt agcgaaggcg gctctctgga acagtactga cactgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004704
VERSION     KHUT01004704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcaggta ggcggcttcg
       61 tgtgtctctt gtgaaagccc tcggctcaac tgaggaattg caaaggaaac tacgaagctt
      121 gagtccggga gaggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggtactga cgctgagctg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004705
VERSION     KHUT01004705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           93 g           53 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggcctga
       61 tgtgtcagat gtgaaagccc tgggcttaac ccaggaagtg catttgaaac tgtcaggctt
      121 gagtagggga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctggc cctatactga cgctgagacg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004706
VERSION     KHUT01004706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           95 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agggcacgca ggcggtcgtg
       61 caagttcggg gtgaaagctc ccggcttaac cgggagaagg cctcgaagac tgcgcgactt
      121 gagagcgaga gagggacacg gaactccgca tggagcagtg acatgcgtag atatgcggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtctctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004707
VERSION     KHUT01004707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           94 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcggtttga
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tgtcagactg
      121 gagcgtggta gaggaaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctggg ccacgtctga cgctcagaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004708
VERSION     KHUT01004708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           83 g           57 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtttga
       61 taagtctgat attaaagact gaggcttaac ctcaggactg tatcggaaac tgtcagacta
      121 gaggacaata gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg gacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004709
VERSION     KHUT01004709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtcagt
       61 caagtcagtt gtgaaagccc tgggctcaac ctgggaatgg cagctgatac tggcggactg
      121 gagtgtaaca gaggaccgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcgat ggcgaaggca gcggtctggg ttaacactga cgctgaggtg cgacggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004710
VERSION     KHUT01004710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c          100 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaagct
       61 taagtgtggt ttgaaagccc ccggctcaac cggggaatgt gattgcaaac tgggcttctt
      121 gagggggata gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg tccgccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004711
VERSION     KHUT01004711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          102 g           42 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcctcg gtgaaaactc cgggctcaac tcggagcctg ccggggaaac tgccgtgctg
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggcgaaggcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004712
VERSION     KHUT01004712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgggtgtctt
      121 gagccaccta ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaat ggtgaagaca gctcgctggg ggtgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004713
VERSION     KHUT01004713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggccacg
       61 taagtcgggt gtgaaatccc ggggctcaac cccgggactg catccgatac tgcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004714
VERSION     KHUT01004714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           87 g           57 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agcgtacgca ggcggtttgg
       61 taagtcagag gtgaaagccc acagcttaac tgtggcactg cctttgatac tgcctttctt
      121 gagtagagta gggggaagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ctctaactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004715
VERSION     KHUT01004715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtccggt gtgaaatttc gaagctcaac ttcgaaactg cattggatac tatccggctc
      121 gaggttggga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagc ggcgaaggcg agtctctgga ccaatcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004716
VERSION     KHUT01004716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcttac
       61 taagtctggt gtgaaagccc acggctcaac cgtggagggc cattggaaac tggtaagctt
      121 gagtgcagaa gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004717
VERSION     KHUT01004717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          103 g           43 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcgcgca ggcggtctct
       61 taagtctgat gtgaaaggcc acggctcaac cgtgggatgg cattggaaac tgggggactt
      121 gagtgcagga gaggggagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004718
VERSION     KHUT01004718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacgtaggga gcgagcgtta cccggaatca ctgggcgtaa agagcgcgta ggcggctggg
       61 taagtctctt gtgaaagctc ccggcttaac tgggagaggt caagggatac tacctagctg
      121 gagggcagta gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctttctggg ctgtacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01004719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004719
VERSION     KHUT01004719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ttgggcgtaa agcgtatgta ggcggacttg
       61 aaagtcggga gtgaaatccc agggctcaac cctggaattg cttccgaaac ttcaggtcta
      121 gtgtcccgga ggggttgacg gaattcctag tgtagaggtg aaattcgcag atattaggag
      181 gaacaccagt ggcgtaggcg gtcaactgga cgggtacaga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004720
VERSION     KHUT01004720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcttgt
       61 tatgtcggtc gtgaaagccc acggctcaac cgtggaagtg caaccgaaac tggcaagctt
      121 gagtcctgac gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gccatctggg aagcgactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004721
VERSION     KHUT01004721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          101 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggctcgt
       61 taagtcgggt gtgaaacccc ggggctcaac cccgggactg catccgatac tggcgggctt
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004722
VERSION     KHUT01004722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          103 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggccgtt
       61 taagttgggt gtgaaagccc ggggctcaac cccgggacgc cgcccaagac tggacggctt
      121 gaggacggaa gaggggcgtg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacacccgt ggcgaaggcg gcgccctggt ccgtttctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004723
VERSION     KHUT01004723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          106 g           41 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggcccgg
       61 cgcgtccgcg gtgaaagctc ccggctcaac tgggagaggt ccgcggaaac ggtcgggctc
      121 gagcaaggta gggggtcgtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cctgtgctga cgctgatgcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01004724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004724
VERSION     KHUT01004724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           83 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtgggaatt
       61 taagtgtgat gttaaatcgt gtggctcaac catatcgatg catcgcatac tgggttccta
      121 gaggacggca gaggcaagtg gaattagcag tgtagcagtg aaatgcgtag atattgctag
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004725
VERSION     KHUT01004725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          103 g           38 t
ORIGIN      
        1 aacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggcggccgct
       61 taagtcctgt gtgaaaggct ccggcttaac cggagacggc catgggaaac tgggcggctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtaactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004726
VERSION     KHUT01004726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           46 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggct gtgaaaacac ggggctcaac tccgtgcctg cagtcgatac gggcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgaaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004727
VERSION     KHUT01004727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           92 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgta ggtggctatt
       61 caagtctgct gttaaagcgc agggcttaac cctgtaaagg cggtggaaac tggagagcta
      121 gagtgtggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaagcg ctctactggg ccacaactga cactgatgga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01004728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004728
VERSION     KHUT01004728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ctgagaatca ctgggtttaa agggtgcgta ggtggcgcgt
       61 taagtccgtt gtgaaagccc tgggctcaac ccaggaactg cttcggatac tggcgcgctt
      121 gaggctagta ggggtcactg gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaacgccaat ggtgaagaca ggtgactggg ctagttctga cactgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004729
VERSION     KHUT01004729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           93 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agggctcgta ggcggtccgt
       61 cacgtcggaa gtgaaaacct agagcttaac tctgggcctg ctttcgatac gggcgaactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004730
VERSION     KHUT01004730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          105 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaagat
       61 taagcgtggc ttgaaagccc ccggctcaac cggggaaggt ggctgcgaac tggtcttctt
      121 gagggcgata gaggggggcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccccctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004731
VERSION     KHUT01004731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggcggcttgg
       61 taagttggac gtgaaaaccc cgggcttaac tcggggaggc cgttcaatac tgccgggctg
      121 gagggcgtca gagggcagtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatccgt ggcgaaagcg gctgcctggg gcgcacctga cgctgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004732
VERSION     KHUT01004732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           95 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggtcctt
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccggatac tgagggacta
      121 gagtgcggga ggggagagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggtgtaaacg gctctctgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004733
VERSION     KHUT01004733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           85 g           51 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgctg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaatac tacagtgctc
      121 gagggagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacctgt ggcgaaagcg gcttactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004734
VERSION     KHUT01004734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           76 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggatctt
       61 taagtcagtg gtgaaagcct gcagctcaac tgtagaacag ccattgaaac tgaagatctt
      121 gaatatggtt aaagtaggcg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat agcgaaggca gcttgctgaa ccattattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004735
VERSION     KHUT01004735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           98 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggcgatc
       61 tacgtctggg gtgaaagccc ggggctcaac ctcgggactg ccttggatac gggatcgctt
      121 gagaatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004736
VERSION     KHUT01004736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c           99 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtccgtg gtgaaatccc ccggctcaac cggggaatgg cctcggatac tggcggtctt
      121 gagggaggta ggggcgtgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004737
VERSION     KHUT01004737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtctgct gtgaaaactc gaggctcaac ctcgagcttg cagtgggtac gggcaggcta
      121 gagtgtggta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gatctctggg ccactactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004738
VERSION     KHUT01004738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtggtttag
       61 taagtcagtg gtgaaagccc cgtgcttaac ttgggaattg ccattgatac tgctaatctt
      121 gagaatgttt gaagtggacg gaatatggca tgtagcggtg aaatgcttag atatgccata
      181 gaacaccgat tgtgaagaca gttcactaag ccatatctga cactgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004739
VERSION     KHUT01004739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           98 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcgga
       61 caagtcggtt gtgaaatctc tgggcttaac ccagagcggc caaccgatac tgtctgacta
      121 gagtgcggaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004740
VERSION     KHUT01004740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggatctg
       61 caagtcagag gtgaaagccc tgggctcaac ccgggaatgg cctttgagac tgcaagtctt
      121 gagtgcgaga gagggtggcg gaatacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccacctggc tcgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004741
VERSION     KHUT01004741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggtttgt
       61 caggtctgat gtgaaatctt ggggctcaac tccaagcgtg catcggaaac cggcttacta
      121 gagtctggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagt tgcgaaggcg gctctctggg acagtactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01004742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004742
VERSION     KHUT01004742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcttg
       61 taagtccgtg gtgaaagcct gctgctcaac agcagaactg ccatggatac tgcaggactt
      121 gagtccagac gaggttggcg gaatagaagg tgtagcggtg aaatgcatag ataccttcta
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004743
VERSION     KHUT01004743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcgggcccg
       61 taagtcagag gtgaaagccc acggctcaac cgtggaattg cctttgatac tgcgggtctt
      121 gagtcctgga gaggctgccg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga tgcgtaggcg ggcagctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004744
VERSION     KHUT01004744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          104 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tggcggactt
      121 gagcgtatca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg atactgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004745
VERSION     KHUT01004745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtgatt
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg cgtttgaaac tggatcgcta
      121 gagtttagca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctagaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004746
VERSION     KHUT01004746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttt
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tggaaagctt
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004747
VERSION     KHUT01004747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcaatg
       61 taagtcggat gtgaaagccc ttggctcaac caaggaagtg catccgatac tgcgtcgctt
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga cgacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004748
VERSION     KHUT01004748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 gacgtagggt gcaagcgtta tccggattta ctgggcgtaa agagcgcgta ggcggatcgt
       61 taagtgtgaa gtgaaatctt cgggctcaac ccgaaaactg cttcgcatac tggcgatctt
      121 gaggggcaca gaggcaactg gaattcctgg tgaagcggtg aaatgcgtag atatcaggag
      181 gaactcccat ggcgaaggca ggttgctggg tgccatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004749
VERSION     KHUT01004749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           43 a           62 c          110 g           38 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtggcg
       61 tatgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac tgcgtcactt
      121 gaggtcggca ggggctggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgtacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004750
VERSION     KHUT01004750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           36 c           79 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttcta
       61 taagtcagtg tttaaagtct gcagctcaac tgtagaagtg gcattgatac tgtagaactt
      121 gagtaaagtt gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag atatcatcaa
      181 gaacatcgat tgcgaaggca gcttactaag ctttaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004751
VERSION     KHUT01004751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggttcga
       61 taagtaggag gtgaaatctc acggctcaac cgggaggctg ccttctagac tgtcggactt
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004752
VERSION     KHUT01004752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtgggctga
       61 taagttcgat gtgaaagccc cgggcttaac ccgggaatgg cattggaaac tgttggtctg
      121 gagggttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caaaccctga cactgaggca cgaaagccaa
      241 gggagcaaac agg
//
LOCUS       KHUT01004753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004753
VERSION     KHUT01004753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           87 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggtggtttga
       61 taagtcagaa gtgaaagccc agggcttaac cctgggactg cttttgaaac tatcagacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004754
VERSION     KHUT01004754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           43 a           65 c          111 g           34 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggctccg
       61 agagcccggc gtgaaagccc ccggctcaac cgggggaggt cgtcggggac ctcggggctg
      121 gaggccggca ggggcgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01004755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004755
VERSION     KHUT01004755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggagtcta ggcggtcagg
       61 taagtggaat gtgaaatccc tcggctcacc cgaggacctg catcccaaac tgcctggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaagct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004756
VERSION     KHUT01004756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           76 g           64 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtccta
       61 taagtcagtg gtgaaagacg gcagcttaac tgtcgcagtg cctttgatac tgtaggactt
      121 gaatctattt gaagtgggcg gaataagaca agtagcggtg aaatgcatag atatgtctta
      181 gaactccgat tgcgaaggca gctcactaag ttagtattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004757
VERSION     KHUT01004757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgtatggt gcgaacgttg ttcggaatca ctgggcataa agagcacgta ggcggttgag
       61 caagtccatt gtgaaatccc ccggcttaac cggggaaccg cagtggatac tacttgactt
      121 gagtacagga ggggagagtg gaattccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacgccggt ggcgaaagcg actctctggt ctgtaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004758
VERSION     KHUT01004758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           76 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagccg gcagctcaac tgtcgaactg ccattgaaac tgatagccta
      121 gaatctagta gaggtaggcg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaagca gcttactatg ctagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004759
VERSION     KHUT01004759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           81 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggtctt
       61 taagtcagtg gtgaaatcct cccgcttaac gggagaactg ccattgatac tattggtctt
      121 gaattcagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004760
VERSION     KHUT01004760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           76 g           59 t
ORIGIN      
        1 tacgtaggtt gcaagcgtta tccggaatta ttgggcgtaa agcgtacgca ggcggtttgt
       61 taagttggaa gtcaaagcta tgagctcaac tcataacagg tttccaatac tggcaagctg
      121 gagataggca gaggaaagtg gaattctgta tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gctttctggg ccttttctga cgctcatgta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004761
VERSION     KHUT01004761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggacagt
       61 taagtccggt gtgaaatccc tcggctcaac tgaggacgtg cactggatac tggcggtctt
      121 gaatcctgga gagggtagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagtgattga cgctgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004762
VERSION     KHUT01004762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           92 g           44 t
ORIGIN      
        1 tacagagagt gcaaacgttg ttcggaatta ctgggcttga agcgcacgta ggcggcgtcg
       61 taagtgtcgg gtgaaatccc acggctcacc cgtggaactg cccggcatac tgcgacgctc
      121 gagtgtggta gggaagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctttggt ccacaactga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004763
VERSION     KHUT01004763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggactct
       61 caagtcggtg gtgaaatcgt tcggctcaac cggacaaggg ccgccgatac tggaagtctc
      121 gagggaggta ggggtcgggg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg cccgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004764
VERSION     KHUT01004764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           89 g           44 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaacgg
       61 aaagtcagat gtgaaatccc cgagctcaac tcgggaactg cacctgaaac ttccgatctt
      121 gagtaccgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004765
VERSION     KHUT01004765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           78 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaattg
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaattg ccattgatac tgcaaatctt
      121 gagtacgttt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaag ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01004766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004766
VERSION     KHUT01004766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggattat
       61 taagtccgtg gtgaaatctt tgggctcaac ccaaaaattg ccattgatac tattagtctt
      121 gaatgtagtt gaggtgggcg gaatgagtca tgtagcggtg aaatgcttag atatgactca
      181 gaacaccaat tgcgaaggca gctcactaaa ctaatattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004767
VERSION     KHUT01004767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           90 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgtccaag
       61 caagtcttgt gtgaaagccc cgagcctaac tcgggaagtg cacaagatac tgtatggatt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctgga cggatactga cgttgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004768
VERSION     KHUT01004768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           79 g           58 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggacttg
       61 taagtcaggg gtgaaatctc acagcttaac tgtgaaactg cctttgatac tgcaagtctt
      121 ggatccagaa gagagttgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcaacttggt ctggtatcga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01004769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004769
VERSION     KHUT01004769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           83 g           57 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggtggttatc
       61 taagtctggt gttaaagacg ggagctcaac tcccgcatgg catcggaaac tggatggcta
      121 gagtgtgaaa gaggtaactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctgt ggcgaaagcg agttactggt tcattactga cactgaggaa cgaaagctag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01004770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004770
VERSION     KHUT01004770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           49 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccct
       61 taagtcggtt gtgaaagctc agggcttaac cctgaaagtg cggccgaaac tgggggactt
      121 gaggcaggta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004771
VERSION     KHUT01004771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacggagggt gctaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggtctgc
       61 taagtcggtt gtgaaagccc ccggctcaac cggggaatag cgttcgatac tggcaggctc
      121 gaggacggtt ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004772
VERSION     KHUT01004772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           46 c          103 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggattgg
       61 tcagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgtttgagac ggctggtctg
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004773
VERSION     KHUT01004773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggagtcta ggcggtcagg
       61 taagtggggc gtgaaatacc tcggctcacc cgaggagctg cgtctcatac tgcctggctt
      121 gagtatggga gaggacaggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cctgtctggc ccaatactga cgctgaagct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004774
VERSION     KHUT01004774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacagagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctga
       61 tgtgtccacg gtgaaatccc tcggctcaac cgaggaatgg ccgtggaaac tgtcgggctc
      121 gaggccggga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004775
VERSION     KHUT01004775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaatccc gaggctcaac ctcgggcttg cagtgggtac gggcagacta
      121 gagtgcggta ggggagaatg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gttctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004776
VERSION     KHUT01004776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          101 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggggaac
       61 caagtccgct gtgaaagcct gaggctcaac ctcagcgggt cggtggatac tggttctctt
      121 gaaggtgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004777
VERSION     KHUT01004777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           90 g           43 t
ORIGIN      
        1 tacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggcggtctt
      121 gggccactca ggggttgccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggcaactggg agtgcgccga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004778
VERSION     KHUT01004778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttga
       61 caagtcttgt gtgaaatcta tgggctcaac ccatagtctg cacgggaaac tgtcgggctt
      121 gagtattgga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga caataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004779
VERSION     KHUT01004779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c           99 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcttgc
       61 accgtcagat gtgaaattcc tgggcttaac ctgggggctg catttgatac gtgtgggctt
      121 gagtgcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004780
VERSION     KHUT01004780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccttt
       61 caagtctctc gtgaaatccc tcggcttaac cgaggacgtg cgggagatac tgtcaggctt
      121 gagtccggaa gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg ccggaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004781
VERSION     KHUT01004781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcgca
       61 caagtcaggg gtgaaagccc acggctcaac cgtggaagtg cctttgatac tgtgtgtctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004782
VERSION     KHUT01004782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           98 g           37 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggcgctg
       61 cgcgtggggt gtgaaagctc ttggcttaac tgagagaggc catcccatac ggcagcgctc
      121 gagccatgta gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg catgagctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01004783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004783
VERSION     KHUT01004783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c          102 g           47 t
ORIGIN      
        1 tacgtaggag acgagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcggatgag
       61 caagtctgct gtgaaagctc ctggcttaac cgggagtggt cggtggaaac tgctcgtctg
      121 gaggacggta gaggtaggta gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaatacctgt ggcgaaggcg gcctactggg ccggacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004784
VERSION     KHUT01004784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggacgat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggtcgcctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctggc cctccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004785
VERSION     KHUT01004785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccatg
       61 caggtccgtt gtgaaaactc gaggctcaac ctcgagacgc cgatggaaac cgcatggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggctaaggcg gctctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004786
VERSION     KHUT01004786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttgtg
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catacaatac tgcacggcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004787
VERSION     KHUT01004787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 cacgggggga gcgagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggctcgg
       61 taagtgggat gtgaaatccc caggctcacc ctgggaacgg catcccaaac tgctgggctt
      121 gagtgcggga gaggatgggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cccatctggc ccgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004788
VERSION     KHUT01004788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          100 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgagta ggcggtctgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccatgcgaac tggtggactt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cgctgaatca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004789
VERSION     KHUT01004789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           84 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggtatca ctgggcttaa agcgttcgta ggcggcctag
       61 taggtgagat gtgaaagccc acggctcaac cgtggaacag cgtttcaaac cactaggctc
      121 gaggatggca ggggtaatgg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg cattactggg ccatttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004790
VERSION     KHUT01004790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           98 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggccgtc
       61 tgcgtcggat gttaaagccc agggcttaac cctgggaggt cattcgatac gggacggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004791
VERSION     KHUT01004791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           59 c          110 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtggct
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcggac tgggtcactc
      121 gagcactgta ggggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004792
VERSION     KHUT01004792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcacta
       61 caagtacgat gtgaaatctc cctgctcaac ggggagggtg cattgtagac tgtagagcta
      121 gagaacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtttctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004793
VERSION     KHUT01004793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgcc gtgaaaacct agggctcaac cctgggcgtg cggtggaaac tgtcgaacta
      121 gaggatggta gaggggagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctccctggg ccattcctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004794
VERSION     KHUT01004794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          105 g           34 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtggcg
       61 caggtcatct gtgaaagccc ccggctcaac cgggcggatg cgggtgaaac cgcgcgactg
      121 gagggtggga gaggtcggcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacccgt agcgaaggcg gccgactgga ccacacctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004795
VERSION     KHUT01004795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggtggcatgg
       61 taagtcagat gtgaaagccc ggggctcaac cccgggattg catttgaaac tatcaagcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004796
VERSION     KHUT01004796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcggtctga
       61 atagtctgct gtgaaagcgc ctggcttaac cgggcgaggt cggcggaaac tctcagactg
      121 gaggccggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccgcgcctga cgctgagacg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004797
VERSION     KHUT01004797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttggat gtgaaatatt ggggctcaac ctcgaaactg cattcaatac tgccttgctc
      121 gagtgctgga taggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagctactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004798
VERSION     KHUT01004798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          109 g           36 t
ORIGIN      
        1 cacgtaggga ccgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggttggt
       61 cgcgtcgggc gtgaaagccc cccgcttaac gggggagggt cgtccgaaac gggccaactg
      121 gaggcaggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactgga cctggcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004799
VERSION     KHUT01004799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           60 c          110 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgctcgca ggcggtcggc
       61 cgcgtccggc gtgaaggccc tcggctcaac cgagggtggt cgtcggaaac gggtcggctg
      121 gaggtaggta gggggacgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggc ggcgaaggcg gcgtcctgga cctggcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01004800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004800
VERSION     KHUT01004800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          100 g           41 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ttgggcgtaa agggcgcgta ggcggccccg
       61 ttagtcaggc gtgaaacccc cgggctcaac ccggggactg cgcttgatac ggcggggctt
      121 gaggacggga gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggc ccgtttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004801
VERSION     KHUT01004801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          101 g           43 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggtggcagtg
       61 caagtatttg gtgaaatctc cctgctcaac ggggagggtg cctagtagac tgcacagcta
      121 gagggcggta gaggggtgtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gcaccctggg ccgcacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004802
VERSION     KHUT01004802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           88 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggcggccaga
       61 taagtcggat gtgaaatccc tgggctcaac ctgggaactg cattcgatac tgtctggcta
      121 gaattcggta gagggtggcg gaactccggg tgtagcggtg aaatgcgtag atatccggaa
      181 gaacaccgat ggcgaaggca accacctggg cctgaattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004803
VERSION     KHUT01004803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggcgagg
       61 aaagtttgag gtgaaatctc caggctcaac ttggaaaatg ccttgaagac ttccttgctc
      121 gagggtcgca gaggaaactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg ggtttctggg cgacacctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004804
VERSION     KHUT01004804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctgat gttaaatact ggagctcaac tccggcatgg cattggaaac tgggcggcta
      121 gagtgtggta gaggtctcgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg cgagactggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01004805             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004805
VERSION     KHUT01004805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           51 t
ORIGIN      
        1 gacgaactgt gcgaacgtta ttcggagtca ctgggcttaa agcgcacgta ggtggcatcg
       61 ttagtctagg gtgaaatacc aacggctcaa ccgtggaatt gccttggata ctgcgaagct
      121 agaggtaggt aggggcgagt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccgg tggcgaaagc gactcgctgg gtctattctg acactgaggt gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01004806             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004806
VERSION     KHUT01004806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcggat gtgaaaactc agggctcaac ccggagacgc catctgatac tgcgatgact
      121 tgagtctggt aggggatcac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccga tggcgaaggc agtgatctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004807             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004807
VERSION     KHUT01004807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           85 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtcaga
       61 taagtcagtt gtgaaatacc ccggcttaac cggcggaact gcgattgata ctgtttgact
      121 tgaatcaggt tgaggtaggc ggaatgtggc atgtagcggt gaaatgctta gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg acctgtattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004808
VERSION     KHUT01004808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 ccagttcggg gtgaaatcct ccggcttaac cggagaactg cctcgaagac gactgatctg
      121 gagggaggta ggggtcgctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agcgactgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004809
VERSION     KHUT01004809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgtgg
       61 caagtcggaa gtgaaatctc tgggctcaac ccagaggctg ctcccgaaac tgccatgcta
      121 gagtgcggga gaggcgagtg gaattacagg tgtagcggtg aaatgcgtag atatctgtag
      181 gaacacccgt ggcgaaggcg gctcgctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004810
VERSION     KHUT01004810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           53 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggag
       61 taagtcaggt gtgaaatctc ggggctcaac cccgaaactg cacttgatac tgcttcgcta
      121 gagtattgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga caattactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004811
VERSION     KHUT01004811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           94 g           47 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 taagtctgcg gtgaaatcct ccagctcaac tggagaactg ccgtggatac tggctgtctc
      121 gaggaaggta ggggcgtgtg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg acacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004812
VERSION     KHUT01004812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttc
       61 caagtcaggg gtgaaatcct ggggctcaac cccagaattg cctttgaaac tgggaagcta
      121 gagtactgga ggggagtgcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gcactctgga cagtaactga cgctctggcg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004813             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004813
VERSION     KHUT01004813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcgggt gtgaaaacta caggctcaac ctgtagatgc cactcgagac tgctatggct
      121 agagtctggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccagtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004814             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004814
VERSION     KHUT01004814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           54 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgaa gtgaaatctc tagggctcaa cccagaaact gctttacata ctggcgagct
      121 ggagtgatga agaggtttgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcaaactgg tcatcaactg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004815
VERSION     KHUT01004815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatagt
       61 taagttagag gtgaaagccc agggctcaac cctggaactg cctttaatac tggctgtcta
      121 gagatcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004816
VERSION     KHUT01004816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           86 g           47 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgtcg
       61 taagtcgggt gtgaaatccc tcagctcaac tgaggaattg cgcccgatac tgcggcgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcattg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctcaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004817
VERSION     KHUT01004817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          100 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcttt
       61 caagtggtgc gtgaaagcgc ccggctcaac cgggcgaggc cgtgccaaac tgagggactg
      121 gaggtaggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg acacgctggg cctgacctga cgctcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004818
VERSION     KHUT01004818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattca ctgggcgtaa agcgcgcgta ggcgggtttt
       61 taagttgggg gtgaaatccc gaggctcaac ctcggaactg cctccaaaac tggaagcctt
      121 gaggtttgga gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004819
VERSION     KHUT01004819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           91 g           43 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 taagtccgtt gtaaaagtcg aaggctcaac cttcgaaagc cgatggatac tgccgggcta
      121 gagttcggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gctcgctggt acgacactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004820
VERSION     KHUT01004820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           76 g           62 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtctta
       61 taagtcagtg gtgaaatctc cccgctcaac ggggaaactg ccattgatac tgtaagactt
      121 gaattattgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccgat tgcgaaggca ggttactaac aatggattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004821
VERSION     KHUT01004821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           81 g           52 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcgtaa agcgtgtgta ggcggtctat
       61 caagtcagga gtgaaatccc agggctcaac cctggaattg ctcttgaaac tgataagcta
      121 gagtactgga gaggttagca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgtaagcg gctaactgga cagtaactga cgctgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004822
VERSION     KHUT01004822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           86 g           53 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggattta ctgggcgtaa agggagcgta ggcggataat
       61 taagtgggat gtgaaatacc cgggcttaac ctgggtgctg cattccaaac tgattatcta
      121 gagtgcagga gaggagagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actctctgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004823
VERSION     KHUT01004823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           39 c           80 g           53 t
ORIGIN      
        1 gacgggggat gcaagtgtta tccggaataa ttgggcgtaa agggtctgta ggtggtttac
       61 caagtctgtt gttaaaaatt agggctcaac cctaatatgg caacagaaac tagtaggctt
      121 gagtacggta ggggcaaagg gaattctcgg tgtagtggtg aaatacgtag atatcgagaa
      181 gaacaccaac agcgaaagca ctttgctagg ccgaaactga cattgagaga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01004824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004824
VERSION     KHUT01004824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcgggatct
       61 taagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgatac tgggattctt
      121 gagttcgaga gaggtgagcg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004825
VERSION     KHUT01004825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           90 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtcggat gtgaaagctc ggagctcaac tccgaaatgg cattggaaac taccttgctt
      121 gagggttgga ggggggactg gaatgcttgg tgtagcagtg aaatgcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004826
VERSION     KHUT01004826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgc
       61 caagtccgtg gtgaaatccc cgagcttaac tcgggaactg ccgtggatac tggtagcctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat agcgaaggca gcttactaca cgatcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004827
VERSION     KHUT01004827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatgtt
       61 taagttaggg gtgaaagccc agggctcaac cctggaattg cctctaatac tggacatctt
      121 gaggccgaga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004828
VERSION     KHUT01004828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcgggtgat
       61 caagtcagat gtgaaagccc taggctcaac ctaggaactg catttgaaac tggttgtctt
      121 gagtattgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga caattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004829
VERSION     KHUT01004829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           86 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggtggatatt
       61 taagtgggat gtgaaatact cgggcttaac ctgggtgctg cattccaaac tggatatcta
      121 gagtgcagga gaggaaagta gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaataccagt ggcgaaggcg actttctgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004830
VERSION     KHUT01004830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcttg
       61 taagtcagtg gtgaaagccc ggggcttaac tccggaactg ccgttgatac tgcaagcctt
      121 gagtacagtt gaggtgggcg gaatacatca tgtagtggtg aaatacttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaaa gtgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004831
VERSION     KHUT01004831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           80 g           62 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatta ttgggtttaa agggtccgta ggcgggccat
       61 taagtcaggg gtgaaagtct gcggctcaac cgtagaattg cctttgatac tggtggtctt
      121 gagttatggt gaagtggcta gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaataccgat tgcgaaggca ggccactaac catatactga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004832
VERSION     KHUT01004832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           93 g           43 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgcc
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaatgg ctgggcatac tgggcgtctt
      121 gagccattca ggggcaactg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaac ggtgaaaaca ggttgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004833
VERSION     KHUT01004833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcgcac
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaactg catttgatac tggtgtgctt
      121 gagttcggtt ggggcgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcgctgga ccgattctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004834             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004834
VERSION     KHUT01004834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           95 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcggagaga
       61 tatgtcggaa gtgaaatcgt gcggcttaac cgtagcaatt gcttccgaaa cggtctccct
      121 tgagtgcgag aggggcagat ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacaccgg tggcgaaggc gatctgctgg ctcgtaactg acgctgaggc gcgaaagtgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004835
VERSION     KHUT01004835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggttttg
       61 taagtccacc gtgaaagctt acggctcaac cgtaacgggt cggtggatac tgcaagactt
      121 gagggcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgttactga cgctgaggcg cgaaagctgg
      241 ggaagtgaac ggg
//
LOCUS       KHUT01004836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004836
VERSION     KHUT01004836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccatg
       61 ttagtcagct gtgaaatccc tcggctcaac cgaggaattg cggctgatac tgcatggctt
      121 gagtcccgga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gctacctgga cgggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004837
VERSION     KHUT01004837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           76 g           58 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggcggattgg
       61 taagtcagag gtgaaatccc acagctcaac tgtggaactg cctttgatac tgtcagtcta
      121 gagtatagtt gaagttggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat cgcgaaggca gctagctaag ctataactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004838
VERSION     KHUT01004838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           97 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggcttgta ggcggttcgt
       61 taagtctggt gtgaaatctc ccggctcaac tgggaggggt cactggatac tggcgaacta
      121 gaggggggta gaggagagca gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccagt ggcgaaggcg gctctctggg cctaccctga cgctgaggag cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01004839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004839
VERSION     KHUT01004839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgcct
       61 caagtttgat gtgaaagctc agggcttaac cctgaaatgg cattgaatac tgaggggctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004840
VERSION     KHUT01004840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          101 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgtccgca ggtggttgtc
       61 taagactgac gtgaaagcgc ctggcttaac tgggcgaggt cgtgggtgac tggacgactg
      121 gaggcaagaa gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaataccaga ggcgcaagcg gcaccctggt cttggcctga cgctgaggga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004841
VERSION     KHUT01004841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           96 g           44 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgcgttcc ggcggcacag
       61 ccagtcgggc atgaaatctc tcggctcaac cgggaggggt tgtccgatac tgctgggctg
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgtacctga cgctgacacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004842
VERSION     KHUT01004842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           94 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttctt
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaatac tgaggagcta
      121 gagtgcggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004843
VERSION     KHUT01004843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatga ctgggcgtaa agggagcgca ggcggctgtt
       61 taagtctgga gtgaaagccc ggggctcaac cccggcgggt cttcggatac tggacagcta
      121 gagtacggaa gaggagagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccgatactga cgctcaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004844
VERSION     KHUT01004844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agcgtacgta ggcggtttgt
       61 taagtcaggg gttaaagact gaggctcaac ctcaggagtg cctttgatac tggcagactg
      121 gaggacggca gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg actttctggg ccgttcctga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004845             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004845
VERSION     KHUT01004845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcttgta ggcggatcgt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tggcgatctt
      121 cgagtccggg agaggtgagt ggaactccga gtgtagaggt gaaattcgta gatattcgga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgcttaaga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004846
VERSION     KHUT01004846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           99 g           47 t
ORIGIN      
        1 gacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctga
       61 tgagtcagac gtgaaaggcc tgggctcaac ctgggaattg cgtttgatac tgttgggcta
      121 gagagcggga gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctacctgga ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004847
VERSION     KHUT01004847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           96 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggcgcat
       61 caagtcgggc gtgaaagccc cgggctcaac ctgggaactg cgttcgagac tggtgtgcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccaat ggcgaaggca gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004848
VERSION     KHUT01004848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttcg
       61 taagctggag gtgaaatccc cgggcttaac ctgggaatgg ccttcaggac tgcgaggcta
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccagcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004849
VERSION     KHUT01004849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctgtc
       61 taagcagggt gtgaaagccc ccggctcaac cggggaattg cgttctgaac tggacggctg
      121 gagtgggata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004850
VERSION     KHUT01004850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           98 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtctgc
       61 gaagtttgga gtgacagttg gtggctcaac caccaaagtg cttcgaaaac tggcagactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggg gagtacctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004851
VERSION     KHUT01004851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttcag
       61 taagtcccgc gtgaaagccc tcggctcaac tggggaattg cgcgggaaac tattggactt
      121 gagttcggga gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004852
VERSION     KHUT01004852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaatgg cttggcatac tggcggtctt
      121 gagccattca ggggctactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggtagctggg gatgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004853
VERSION     KHUT01004853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 tcagtcggat gtgaaatcgc tcagctcaac tgagcaactg cgtctgatac taccgtgctt
      121 gaggaaggca gaggtgagcg gaactcaggg tggagcggtg aaatgcgttg atatcctggg
      181 gaacaccggt ggcgaaggcg gctcactggg ccttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004854
VERSION     KHUT01004854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcatgta ggcggctttg
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgatac tgcagagcta
      121 gagtatcgga gagggtcgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gcgacctgga cgaatactga cgctcagatg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004855
VERSION     KHUT01004855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgtgcgg
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaactg cgcccaaaac taccgtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004856
VERSION     KHUT01004856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           94 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcatgca ggcggtccgc
       61 caagtttgag gcgacaatca ctggcttaac tggtgaacgc cctcaaaaac tggaggactt
      121 gagagcgcga gagggacacg gaactccggg tggagcagtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc gcgtctctga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004857
VERSION     KHUT01004857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           49 t
ORIGIN      
        1 gacgtagggg gcgagcgtta ttcggaatta ttgggcgtaa agcgtgcgta ggcggattcg
       61 taagtcgggg ttgaaatatc cgggctcaac tcggggcacg gcctcgatac tgcggatctc
      121 gagtcgagaa gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggt cttgcactga cgctgatgca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004858
VERSION     KHUT01004858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           77 g           58 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggacttg
       61 taagtcagtg gtgaaatctg tgggcttaac ccacaaactg ccattgatac tgcaggtcta
      121 gagtacagtt gccgttggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaag ctgtaactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004859
VERSION     KHUT01004859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 tacgggaggt gcaagcgttg ttcggagtga ctgggcgtaa agcgcacgta ggcgggtttg
       61 taagtcggat gtgaaatccc taggcttaac ctaggaactg catccgaaac tataagtctt
      121 gaatacctca gaggttggta gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaataccgga ggcgaaggcg gccaactggg agagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004860
VERSION     KHUT01004860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcatgg
       61 caagtcgggt gtgaaatccc ggggctcaac cccggaactg cactcgatac tgccgtgctt
      121 gagtgttgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccggt ggcgaaggct agtctctgga caactactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004861
VERSION     KHUT01004861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           92 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgg
       61 taagtccgct gtaaaagtcc agggctcaac cctggaaagc cggtggaaac taccaagctt
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004862
VERSION     KHUT01004862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           54 c          106 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctttt
       61 taagggtgcg gtgaaagtcc ggggcttaac cccggatcgg ccgtgccgac tgggaggctt
      121 gagcactgta gaggcgagtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg cagtggctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004863
VERSION     KHUT01004863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           93 g           51 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggtaagctt
      121 gagtgaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004864
VERSION     KHUT01004864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           89 g           56 t
ORIGIN      
        1 tacgtagggt gcgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggatcg
       61 taagttagga gtttaataca tgggctcaac ccgtgatatg cttttaatac tgcggttctt
      121 gagtatggga gagggtgatg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt agcgaaggcg gtcacctggc ccaatactga cgctgaggct cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004865
VERSION     KHUT01004865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           92 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttga
       61 ttagtcagat gtgaaattcc cgggcttaac ctgggggctg catttgatac tgtcaggctt
      121 gaattcggga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctatctgga ccgacattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004866             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004866
VERSION     KHUT01004866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           98 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcgggctgt
       61 atagtcagat gtgaaattcc cgggctcaac ctgggggcgg catttgatac gtatggtctt
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttttactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004867             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004867
VERSION     KHUT01004867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcgc
       61 taagtcaggt gtgaaatctc cgcgctcaac gcggaggggc cacctgatac tggcgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004868             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004868
VERSION     KHUT01004868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           99 g           51 t
ORIGIN      
        1 tacggaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggggat
       61 taagtgagaa gtgaaaggct ggggctcaac cccatgcagt gcttctcata ctggtcttct
      121 tgagggtggt agagagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccatttctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004869             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004869
VERSION     KHUT01004869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          110 g           34 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggtcctg
       61 gaacgcgtgc cgtgaaagcc cggggctcaa ccccgggtcg gcggtgcgaa cggggggact
      121 ggagcacacc agaggcaggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcctgctgg ggtgtggctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004870
VERSION     KHUT01004870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcttgg
       61 taagtcaggg gtgaaagctt cccgctcaac gggagaactg cccttgatac tgccaggctc
      121 gaattgggtt gaggcaggcg gaatgtggca tgtagcggtg aaatgcttag atatgccata
      181 gaacaccgat tgcgaaggca gcctgccaag ccttgattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004871
VERSION     KHUT01004871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           99 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggctcgt
       61 taagtcagac gtgaaagccc tcggcttaac cggggaacgg cgtctgagac tggcaagctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004872
VERSION     KHUT01004872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggcttcg
       61 caagtcaggc gtgaaatccc cgagcttaac ttgggaattg cgtttgaaac tacgaagctg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004873             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004873
VERSION     KHUT01004873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           92 g           44 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgggtcgtgg gtgaaatcct ccagcttaac tggagaactg ctgacgatac cactgcggct
      121 cgaggaagga aggggcaatc ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgccgg tggcgaaagc ggattgctgg tccttttctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01004874             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004874
VERSION     KHUT01004874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagacgc cacccgatac tgtcgtgact
      121 ggagtccggt aggggagtat ggaatttccg gtgtagcggt gaaatgcgca gatatcggaa
      181 ggaacaccag tagcgaaggc ggtactctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004875             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004875
VERSION     KHUT01004875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 gacgtcggtt gctgaaatcc cccggctcaa ccggggaacg ggcaccgaaa cgccccgtct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttttctg acgctgagac gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01004876             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004876
VERSION     KHUT01004876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           98 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggcttct
       61 taagtccggg gtgaaaggtt cagcgctcaa cgcgaacagt gccttggata ctggggggct
      121 tgaatgtggc agaggatagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccca tggcgaaggc agctatctgg gctaacattg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004877             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004877
VERSION     KHUT01004877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagacgc cactcgatac tgctgtgact
      121 ggagtccggt aggggagcat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc gatgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004878
VERSION     KHUT01004878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c          101 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcttgc
       61 acagtcaggc gtgaaattcc tgggctcaac ctggggactg cgcttgagac gtgtgggctt
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004879
VERSION     KHUT01004879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatttgtctt
      121 gaatattctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg gaactattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004880
VERSION     KHUT01004880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           95 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgct
       61 caagtctgtg gtgaaatcct ccggctcaac cggagaacgg ccatggatac tgggtgcctc
      121 gagggtgata ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga tcacttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004881
VERSION     KHUT01004881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           57 c          108 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcttcg
       61 caagtcggcc gtgaaatctc cgggctcaac tcggaggggt cgggcgagac tgcgaggctc
      121 gaggtgggca gagggcagtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctgcctggg cctggcctga cgctgaggcg cgaaagcgtg
      241 gggagcaatc cgg
//
LOCUS       KHUT01004882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004882
VERSION     KHUT01004882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggtccgt
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaacgg ctgggcatac tggcgggctt
      121 gagacactca ggggtcagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccgat ggtgaaaaca gctgactggg ggtgttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004883             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004883
VERSION     KHUT01004883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           46 t
ORIGIN      
        1 cacgtaggca ccaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagaggc cactcgatac tgctgtgact
      121 agagtgcggt aggggagcgg ggaattcatg gtgtagcggt gaaatgcgca gatatcatga
      181 ggaacaccag cggcgaaggc gccgctctgg gccgtaactg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004884
VERSION     KHUT01004884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           74 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcggatatt
       61 taagtccgag gtgaaatctc tgagctcaac tcagaaactg ccttggatac tatttatctt
      121 gaatatcgtt gaggcaggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcttgctaaa cgattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004885             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004885
VERSION     KHUT01004885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          100 g           41 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctcg
       61 taagtcgcgt gtgaaaggcc ggggctcaac cccgagcact gcacgcgata ctgcatggct
      121 ggagagaggt aggggcgagc ggaattcccg gtgtagcggt ggaatgcgta gatatcggga
      181 agaacacctg tggcgaaggc ggctcgctgg acctcttctg acgctgaagc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004886
VERSION     KHUT01004886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcaagca ggcggcgacc
       61 taagtctgac gtgaaatctc ctggcttaac tgggagaggt cgttggaaac tgggttgctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaga ggcggaagcg gcaccctggc attagcctga cgctgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004887
VERSION     KHUT01004887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           89 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggcctt
       61 caagtcggac gtgaaagccc tgagctcaac tcaggaactg catccgaaac tgaaggcctg
      121 gagtagtgga gagggaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004888             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004888
VERSION     KHUT01004888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccagattta ctgggcgtaa agcgcgtgta ggcggctggt
       61 taggtgtgat gtgaaatcct cgggctcaac ccgagaactg cattgcaaac cggcctggct
      121 agagtgcagg agagggaagc ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccag tggcgaaggc ggcttcctgg cctgtaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01004889             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004889
VERSION     KHUT01004889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggcttta
       61 taagtcattg gtgaaagctc gcagcttaac tgtgaaactg ccaatgaaac tgtttagctt
      121 gaatgtggtt gaagtgggcg gaatacaaca tgtagcggtg aaatgcttag atatgttgta
      181 gaacaccgat tgcgaaggca gctcactaag ccaattattg acgctgaggc acgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01004890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004890
VERSION     KHUT01004890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           86 g           53 t
ORIGIN      
        1 tacgaggggt gcgagcgtta ttcggaatta ttgggcgtac agggcgcgta gacggcatat
       61 taagtcaact gttaaattcc ttggcctaac tgaggaccag cagtagatac tgatgtgctc
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004891
VERSION     KHUT01004891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtgtac
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tgggacacta
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004892
VERSION     KHUT01004892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 tcagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgcctggcta
      121 gagtgtgata gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaggca gccacctggg tcaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004893
VERSION     KHUT01004893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           90 g           48 t
ORIGIN      
        1 aacgtaggtt ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggctcag
       61 caagtcgggc atgaaatctc tcggctcaac cgggaggggc tgtccgaaac tattgggctt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccacctgga ctgtacctga cgctcaaaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004894
VERSION     KHUT01004894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           85 g           60 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg ttcggattta ttgggcgtaa agggcgcgta ggcggatagt
       61 taagttgggt gtttaaccca caggctcaac ctgtggtctg cattcaatac tgattatctt
      121 gagtatcgga ggggaaagtg gaattcttgg tgtagcggtg gaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg actttctggc tgaatactga cgctaaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004895
VERSION     KHUT01004895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 tacgtaggga gcaagcgtta ctcggaatca ctgggcataa agggcgtgta ggcgggtgcg
       61 taagtccggt gtgaaatccc tcggctcaac cgaggaaggc catcggatac tgcgtgtcta
      121 gagtgtggaa ggggagagtg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg gctctctggt ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaag ggg
//
LOCUS       KHUT01004896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004896
VERSION     KHUT01004896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           95 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgc
       61 tcagtcagat gtgaaagtcc acggctcaac cgtggaagtg catttgatac tggcagactt
      121 gagtactgga gggggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg accatctggc cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004897
VERSION     KHUT01004897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           78 g           56 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgca ggcggactaa
       61 taagtcagtg gtgaaagcct gatgcttaac atcagaactg ccattgatac tgttagtctt
      121 gagtacggtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004898             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004898
VERSION     KHUT01004898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctct
       61 gcaagtcagt tgtgaaatct ccgggcttaa ctcggaaagg tcaactgata ctgcagggct
      121 agagtacaga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgatactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004899
VERSION     KHUT01004899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           92 g           53 t
ORIGIN      
        1 cacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 taagttggat gtgaaatctc ctggctcaac tgggagaggt cgttcaatac taccagacta
      121 gaggacggta gaggaaggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctggg ccgttcctga cgctcagacg cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004900
VERSION     KHUT01004900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggggaag
       61 caagtccggt gggaaagccc gcggctcaac cgcggaagtt cgctggaaac tgctactctt
      121 gagggtgata gagggaagtg gaattgctgg tgtagcggtg aaatgcgtag atatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctgga tcacccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004901
VERSION     KHUT01004901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           89 g           45 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taggtttcgt gttaaatccc tcggctcaac cgaggaatcg cacggaaaac cacctcgcta
      121 gagtccggaa gagggaaacg gaattccagg tgtagcggtg aaatgcgcag atatctggaa
      181 gaacacccgt ggcgaaggcg gtttcctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004902
VERSION     KHUT01004902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          103 g           42 t
ORIGIN      
        1 gacagaggag gcaagcgttg tccggattga ctgggcgtaa agggcacgca ggtggtcgtg
       61 caagtgccgt gtgaaagcga cctgcttaac gggtcgaggc catggcagac tgctcgactg
      121 gagctgtgca gaggtccgtg gaattgccgg tgtagtggtg atatgcgtag agatcggcag
      181 gaacaccgag ggggaagcca gcggactggg cacgtgctga cactgaggtg cgaaagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01004903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004903
VERSION     KHUT01004903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggtgcgg
       61 caagtcagat gtgaaatccc ggggctcaac cccggaactg catttgatac tgccggactt
      121 gagttctgga gaggtgtgtg gaattcatgg tggagcagtg aaatgcgtgg atatcatgag
      181 gaacgctaat ggcgtaggca gcacactgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004904
VERSION     KHUT01004904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           77 g           59 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggactta
       61 taagtcagtg gtgaaagcct gttgcttaac aacagaactg ccattgatac tgtaggtctt
      121 gagtacagtt gaggtgggcg gaatgtgtca agtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaag ctgtaactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004905
VERSION     KHUT01004905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggctgtctat
       61 caagtctgat gtgaaatccc agggctcaac cctgggcgtg cattggaaac tggtggactg
      121 gagtttggga gaggctagtg gaattccagg tgtaggggtg aaatccgtag agatctggag
      181 gaacaccggt ggcgaaggcg actggctgga ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004906
VERSION     KHUT01004906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           88 g           55 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgtgta ggcggctagc
       61 taagtagggt gtgaaaggcc ttggcctaac caaggcattg cgctctaaac tgactggctt
      121 gagtaagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tctttactga cgctgagaca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004907
VERSION     KHUT01004907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          102 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgtgta ggcggcttgc
       61 taagccgcgt gtgaaatccc ggggctcaac cccggaacgg cacgcgggac tggcaggctc
      121 gagtgtggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004908
VERSION     KHUT01004908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccaag
       61 caagttggat gtgaaagccc tgggctcaac ccaggaagtg catccaaaac tgcttggctt
      121 gagtaccgga ggggctcaga gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgagctgga cggttactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004909             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004909
VERSION     KHUT01004909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           45 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggcggtctgt
       61 taagtcattt gtgaaaaccc ggggctcaac cccgcggaat gcagatgata ctggcagact
      121 agagaccagt agaggctagc ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacacctg tggcgaaggc ggctagctgg gctggatctg acgctcatcc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004910
VERSION     KHUT01004910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggta ggtggttcgg
       61 caagtctgtt gtggaagctc ctggctcaac cgggagaggc caatggatac taccgagctt
      121 gaatgtggga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccgat ggcgaaagca ggtgactaga ccactattga cactgagcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004911             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004911
VERSION     KHUT01004911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           50 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctggcgagct
      121 tgaggaatgc agaggtaagt agaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaataccga cggcgaaggc agcttactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004912
VERSION     KHUT01004912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaata
       61 caagtcggat gtgaaatccc gcggcttaac cgtggaagtg cacccgaaac tgtatcgcta
      121 gagtcccgga gaggatggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004913
VERSION     KHUT01004913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           88 g           56 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttatt
       61 taagtctggt gttaaatact ggagctcaac ttcggcatgg cactggaaac tggatggcta
      121 gagtgtagca gaggttacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtaactggg ctataactga cgctgaggaa cgaaagccgg
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01004914             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004914
VERSION     KHUT01004914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           49 t
ORIGIN      
        1 cacgtaggca ccaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 tcagtcgggt gtgaaaactt gaggcttaac ctcaagaggt cactcgatac tactgtgact
      121 agagtgcggt aggggagcgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc gccgctctgg gccgtaactg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004915
VERSION     KHUT01004915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           83 g           52 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagtcaggg gtgaaatccc acagcttaac tgtggaactg cctttgaaac tgcaaggcta
      121 gaatgtgaga agggatggcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacacctgt ggcgaaagcg gccatctgga tcacaattga cgctgaagtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004916
VERSION     KHUT01004916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatcct agagcttaac tctagaactg ccgttgatac tatctgtctt
      121 gaataccgtt gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgctaaa cgaatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004917
VERSION     KHUT01004917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgctgga gaggttatta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccaga ggcgaaggcg gataactggc cagacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004918
VERSION     KHUT01004918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           82 g           63 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtctat
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgatggactt
      121 gagtgaagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccaat agcgaaggca gcttactagg ctttaactga cactgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004919
VERSION     KHUT01004919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           82 g           56 t
ORIGIN      
        1 gacgagggat ccaagcgttg ttcggaatta ttgggcgtaa agcggatgca tgtggctcta
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg catttgatac tgtagagctt
      121 gagtgctaga gaggtaagta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccgga ggcgaaggcg gcttactggc tagacactga cactcagatc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004920
VERSION     KHUT01004920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgagta ggcggccttc
       61 taagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tgggaggcta
      121 gagtttggga gagggaggta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcctcctgga ccaatactga cgctgatccg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004921
VERSION     KHUT01004921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 gacgagccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac taccgtactt
      121 gaggaagata gaggtgagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacacccgt ggcgaaagcg gctcactggg tcttttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004922
VERSION     KHUT01004922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           82 g           52 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggtatgc
       61 taagtcagtt gttaaattct ccggcctaac cggaggccag caacagaaac tggtgtacta
      121 gagggtggaa gagagaagcg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttcaga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004923
VERSION     KHUT01004923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcttga
       61 aaagttcgat gtgaaatctc cgggcttaac ccggaaaagg cattgaatac taatgagctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01004924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004924
VERSION     KHUT01004924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtatta
       61 tgggtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac tatagaactg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004925
VERSION     KHUT01004925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 caagtcaact gtgaaagccc aaggcttaac cttggaagtg cagctgatac tgttagattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004926
VERSION     KHUT01004926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           84 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggctata
       61 taagtcagat gtgaaatacc tgagcttaac ttggggggtg catttgatac tgtatagcta
      121 gagttagtca gaggggggta gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaataccaat ggcgtaggca gccccctggg acaaaactga cgctcatgta cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01004927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004927
VERSION     KHUT01004927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctggg
       61 taagtttgag gtgaaagctc cgggctcaac ccggaaattg ccttgaatac tattcggcta
      121 gagggttgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caacatctga cactgaggca cgaaagccaa
      241 gggagcaaac ggg
//
LOCUS       KHUT01004928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004928
VERSION     KHUT01004928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctag
       61 taagtcggcg gtgaaagccc ggggctcaac cccggaactg ccatcgatac tgttaggctt
      121 gagtacaggc gaggttggcg gaatgggcgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctgactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004929
VERSION     KHUT01004929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           76 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaatgg
       61 taagtcagtg gtgaaagccc ttcgcttaac gaaggaactg cctctgatac tgccaatctt
      121 gagtacattt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004930
VERSION     KHUT01004930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           84 g           54 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgtc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaattg cttggtatac tggatgtctt
      121 gagctagcta ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg gctatgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004931
VERSION     KHUT01004931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           75 g           60 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggcccta
       61 taagtcagtg gtgaaatctc tcggcttaac cgagaaattg ccattgatac tgtagggctt
      121 gagtatagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctgacgaaa ctataactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01004932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004932
VERSION     KHUT01004932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 taagtcagat gtgaaaaccc agggcttaac tttgggagtg catttgaaac tgaagggctc
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004933
VERSION     KHUT01004933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 caagtcaagg gtgaaagcct ccagctcaac tggagaagtg cctttgatac tggtcatctt
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004934
VERSION     KHUT01004934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgggtgtctt
      121 gagccactta ggggttaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtaactggg agtgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004935
VERSION     KHUT01004935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggttcg
       61 caagtaggat gtgaaagccc caggctcaac ctgggaatgg caccctaaac tacggacctt
      121 gaatgcttga gaggttggcg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaatatcgga ggcgaaggcg gccaactggt ggtgcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004936
VERSION     KHUT01004936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctgtg
       61 caagtcagat gtgaaagccc tgggctcaac ccaggatgtg catttgatac tgcacggcta
      121 gagtgtcgga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg accatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004937
VERSION     KHUT01004937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           98 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggctttt
       61 taagtcaggc gtgaaagccc cgggctcaac ctgggaacgg cgcttgatac tggaaggctt
      121 gaggacggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004938
VERSION     KHUT01004938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgccg
       61 taagttaggt gtgaaatccc ggggcttaac cccggaactg cacttgatac tgcggtgctt
      121 gagtactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaggcg ggtctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004939
VERSION     KHUT01004939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 gacgtaggtg gcaagcgtta ttcggaatta ctaggcgtaa agcgcgtgta ggcgggcggt
       61 taagtctgcc gtgaaatctc ctggctcaac taggaggggt cggcggaaac tggctgtctt
      121 gagtgaggta ggggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtctctggg cctttactga cgctaagacg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004940
VERSION     KHUT01004940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           93 g           40 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggttctg
       61 ccagtctgat gtgaaatctc ctcgctcaac ggggagcagc cattggaaac tacagaactg
      121 gagtctgaca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg tcagtactga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004941
VERSION     KHUT01004941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggtccgt
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaatgg catttgatac tggcggactt
      121 gaggatgaga tgggtgggcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcccactgga ccatcactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01004942             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004942
VERSION     KHUT01004942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtggt gtgaaatctc cagggctcaa cccggaaact gcaccgcata ctggcgagct
      121 tgagggatgc agaggtggat ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacacccg tggcgaaggc ggtccactgg gcatcatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01004943             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004943
VERSION     KHUT01004943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           97 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggttatg
       61 acaagtcggt tgtgaaatct ccgggcttaa ctcggaaagg tcaaccgata ctgtcagact
      121 agagtgcgga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01004944             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004944
VERSION     KHUT01004944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           87 g           47 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agagtctgta ggcggttgct
       61 cgcgtgagat gcgaaatctg gtggctcaac catctagact gtatctcata cggagcgact
      121 agaggacggc agaggcaagt ggaactagcg gtgtaggggt gaaatccgta gatatcgcta
      181 agaacaccaa tggcgaaggc agcttgctgg gccgcccctg acgctgagag acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004945             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004945
VERSION     KHUT01004945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcaga
       61 ccaagtcgag tgtgaaattc cagggcttaa ctctggcacg ctcgctcgat actggtcggc
      121 tagagtgtgg aagaggatgc tggaattccc ggtgtagcgg tgaaatgcgt agatatcggg
      181 aggaacacca gtggcgaagg cgggcatctg ggccaacact gacgctgagg cgcgaaagct
      241 aggggagcaa acagg
//
LOCUS       KHUT01004946             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004946
VERSION     KHUT01004946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           81 g           59 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggcggcatga
       61 taagttattg gcgggaaatc gaggctcaac ctcgtggaaa ctaataatac tgttttgctt
      121 gagtacagga gaggttaacg gaactccatg tggagcggta aaatgtgtag atatatggaa
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacgttgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01004947             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004947
VERSION     KHUT01004947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           39 c           80 g           58 t
ORIGIN      
        1 gacataggtg acgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggttcga
       61 taagttactg gtgggaaatc gaggcccaac ctcgtggaag ctggtaatac tgtcgagctt
      121 gagtgcagga gaggttaacg gaactttatg tggagcggta aaatgcgtag atatatgaaa
      181 gaacaccgat atagcgaagg cagttaacta ttctgctact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01004948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004948
VERSION     KHUT01004948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggtggcctgt
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tggcaggctt
      121 gagtactgaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg cagatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004949             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004949
VERSION     KHUT01004949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           77 g           59 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtttgta ggcggaagtt
       61 taagtgagat gcgaaatctt gtggctcaac cacatagacg gtatctcata ctgggcttct
      121 agaatatagc agaggtaagt ggaattagta gtgtagcggt aaaatgcgta gatattacta
      181 ggaacaccaa tggcgaaggc agcttactgg gctattattg acgctgagaa acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004950             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004950
VERSION     KHUT01004950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcgatg
       61 taagtctgct gttaaagcgt ctagctcaac tagataaaag cagtggaaac tacttacgct
      121 agagtgcgtt cggggcagag ggaattcctg gtgtagcggt gaaatgcgta gagatcagga
      181 agaacaccag tggcgaaggc gctctgctag gccgcaactg acactgaggg acgaaagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01004951             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004951
VERSION     KHUT01004951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctatt
       61 taagtctggt gtttaaacct tgggctcaac ctgaggtcgc actggaaact gggtggcttg
      121 agtacagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004952             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004952
VERSION     KHUT01004952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctatt
       61 taagtcttat gtataaaccc aaggctcaac cttggatcgc atgggaaact gggtggcttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004953             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004953
VERSION     KHUT01004953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggcggccctt
       61 taagtctcgt gtttaaaccc ggagctcaac ttcgggtcgc acgggaaact ggggggcttg
      121 agtgcaggag agggaagcgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg cttcctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004954             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004954
VERSION     KHUT01004954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           91 g           49 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgca ggcggcgcgc
       61 caagtcagcg gtcaaagttc cgggctcaac ccggtgtcgc cgttgaaact ggcgtgctcg
      121 agtgcgtgcg aggaaggcgg aatgcgttgt gtagcggtga aatgcataga tatgacgcag
      181 aactccgatt gcgaaggcag ctttccagcg cgctactgac gctgaggcac gaaagcgtgg
      241 ggatcgaaca gg
//
LOCUS       KHUT01004955             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004955
VERSION     KHUT01004955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           87 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ttgggcgtaa agagtgcgta ggtggttttg
       61 taagcgcgag gtgaaaggct atggctcaac catagtaagc cttgcgaact gcaagacttg
      121 agtgcaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctttctggac tgtaactgac actgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01004956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004956
VERSION     KHUT01004956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           85 g           52 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcgcgc
       61 taagtcagtg gtgaaagacg gtggctcaac catcgcagtg ccgttgatac tggcgcgctt
      121 gaattcggat gaggtaggcg gaatgtggca agtagcggtg aaatgcatag atatgccaca
      181 gaacaccaat tgcgaaggca gcttaccaag gcgatattga cgctgatgca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01004957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004957
VERSION     KHUT01004957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           84 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agggtacgca ggcggctagg
       61 aaagaaggat gtgaaatccc agggctcaac cttggaactg cattcttaac tgcctagcta
      121 gagtatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataatactga cgctcaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004958
VERSION     KHUT01004958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           99 g           38 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ctcggtatca ctgggcttaa agggcgcgta ggcggtgcgg
       61 tacggtttcg gtgaaatccc tcggcccaac cgaggaacag ccgggacaac ggccgcgcta
      121 gacggcatca ggggacagtg gaactcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg actgtctggg gtgcctgtga cgctgaggcg cgaaagccag
      241 gggatcaaac ggg
//
LOCUS       KHUT01004959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004959
VERSION     KHUT01004959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           87 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggtggccacg
       61 taagtcggtt gtgaaatccc tgggctcaac ccgggaattg cttccgatac tgcgaggctc
      121 gagttcggta gagggtggca gaactcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaataccgat ggcgaaggca accacctggg cctgaactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004960
VERSION     KHUT01004960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           90 g           53 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggattta ctgggcgtaa agcgcttgta ggtggtcttg
       61 taagttgggc gtgaaaacac cgggcttaac tcggtgacgc cgtccaatac tgcgaggctg
      121 gaggatgtca gaggaaggtg gaattcctgg tgtagtggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gccttctggg gcattcctga cactgagaag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004961
VERSION     KHUT01004961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           86 g           50 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttac agagttcgta ggcggctcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaattg cgctcaaaac tactgagctt
      121 gaggggtata gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactggg tacctcctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004962
VERSION     KHUT01004962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttca
       61 caagctagat gtgaaagccc cgggcttaac ctgggaactg catttaggac tgtggagcta
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004963
VERSION     KHUT01004963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtgcttt gtgaaatccc acggcttaac cgtggaattg cttggcatac tgggtgtctt
      121 gagccatcta ggggcagctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggctgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004964
VERSION     KHUT01004964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           90 g           44 t
ORIGIN      
        1 gacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccacg
       61 taagtagggt gtgaaatccc ccggcccaac cggggaactg cgctctaaac tacatggctt
      121 gagtgagcta ggggtccacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga gccgaaaggg gtggactggg gctcaactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004965
VERSION     KHUT01004965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          109 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgtgta ggcggggggg
       61 caagtcgggc gtgaaagcct ccggctcaac cggggaaggg cgctcgagac tgccgttctt
      121 gagggttgga gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggc caagccctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004966
VERSION     KHUT01004966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           91 g           45 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcccta
       61 caagtcaggt gtgaaatccc acggctcaac cgtggaactg cgcctgaaac tgtagggctt
      121 gagggagata ggggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggc ggcgaaggcg gctcactggg tctctactga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01004967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004967
VERSION     KHUT01004967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           85 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccaga
       61 taagtcagtg gtgaaagttt ccggctcaac cggggaactg ccattgatac tgtttggctt
      121 gagtgttgat ggggtacatg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcgaaggca ttgtactgat cagcaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01004968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU4999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004968
VERSION     KHUT01004968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU4999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           86 g           50 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtctgt
       61 taagttggat gttaaaactc ccggctcaac tgggagctgt cgttcaaaac tagcagacta
      121 gaggacggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcctcctggc ccgctcctga cactaagacg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004969
VERSION     KHUT01004969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtctgct gtgaaatccc aaggcttaac cttgggcctg cagtgggtac gggcaagcta
      121 gagtgcggta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gatctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01004970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004970
VERSION     KHUT01004970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcagt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatctgtctt
      121 gaatatcgtt gaggtgggca gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaataccgat tgcgaaggca gcttgctaaa cgatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004971
VERSION     KHUT01004971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           86 g           50 t
ORIGIN      
        1 gacgaacctg acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtag
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaatac tgctatgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt agcgaaagcg gctcactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004972
VERSION     KHUT01004972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           81 g           56 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggacttg
       61 ttagtcagag gtgaaatctc acggctcaac cgtgaaactg cctttgatac tgcaggtctt
      121 gaattgggga gagagttgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcaacttggc cccagattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01004973             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004973
VERSION     KHUT01004973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           43 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 taagtcaggt gtgaaacccc cgggctcaac ccggggacgc cacctgatac tgctatggct
      121 tgagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01004974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004974
VERSION     KHUT01004974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           87 g           48 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcggcg
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac tgcgtcgctt
      121 gaggaagata gaggtgagtg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaagcg actcactggg tcttctctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01004975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004975
VERSION     KHUT01004975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           92 g           49 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggatttg
       61 taagtctact ctgaaagctt ctggctaaac tggaagaggc gagtggagac tgcaagtcta
      121 gagtgaggaa gaggaaagtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaagcg gctttctggt ccttaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004976
VERSION     KHUT01004976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           87 g           47 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggttcag
       61 taagtctgtt gtggaagctc ccggcttaac cgggagaggc caacggaaac tactggactt
      121 gagtgtagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ctattactga cactaagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01004977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004977
VERSION     KHUT01004977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtccct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tgggggactt
      121 gagtgcagga gaggggagcg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004978
VERSION     KHUT01004978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggagcgta ggtggtcttt
       61 taagtgggat gtgaaatccc tgggcttaac ccaggaattg cattccatac tgggagacta
      121 gagtgcagga gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaaccccagt ggcgaaggcg actttctgga ctgtaactga cactgaggct cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004979
VERSION     KHUT01004979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           84 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggagtta ctgggcgtaa agagcgcgta ggcggtcttt
       61 taagtcattt gttaaatctc ccggcttaac cgggaacatg caggtgaaac tggaagacta
      121 gagtgtaaga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgga ggcgaaggcg gcttcttggc ttacaactga cgctcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004980
VERSION     KHUT01004980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcacga
       61 cgtgtccaag gtgaaatccc tcggctcaac tgaggaactg ccttggaaac tgtcgagctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01004981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004981
VERSION     KHUT01004981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcgggg gtgaaatctt gccgcttaac ggcaaaagag ctttcgatac tggcgagctg
      121 gagggggata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tccccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004982
VERSION     KHUT01004982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           91 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgtgca ggcggtttgc
       61 taagatagag gtgaaatccc cgggcttaac ctgggaatgg cctatatgac tggcaagctc
      121 gagtagggca gagggaggtg gaattccaca tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg cccatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004983
VERSION     KHUT01004983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggcc
       61 caagtcaacg gtgaaagctc ggcgctcaac gccgagtcgg ccgtcgatac tggggcgctt
      121 gagcactgta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004984
VERSION     KHUT01004984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggcggat
       61 taagtcgaat gttaaagcct ggggctcaac cccagagatg cactcgatac tggtctgcta
      121 gagggtgata gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacatcagt agcgaaggcg actttctggg tcacacctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01004985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004985
VERSION     KHUT01004985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggctttg
       61 ttagtctggt gtgaaatctc ccggcttaac tgggagggtg cgctagaaac tgcagagcta
      121 gagtatcgga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga cgatcactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004986             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004986
VERSION     KHUT01004986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           99 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 ttagtcgggt gtgaaacccc cgggctcaac ccggggacgc cacccgatac tgctgtggct
      121 agagtccggt aggggagcgc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004987
VERSION     KHUT01004987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           76 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaatag
       61 taagtcagtg gtgaaagcct gttgcttaac aacagaactg ccattgatac tgctaatctt
      121 gagtacattt gatgtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgttactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01004988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004988
VERSION     KHUT01004988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           96 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttga
       61 tgggtgtgat gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac tattaaactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01004989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004989
VERSION     KHUT01004989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           82 g           53 t
ORIGIN      
        1 tacgagaggt gcaaacgtta ttcggaatta ttgggcgtaa agggtgcgta gacggcataa
       61 taagtctgtt gttaaaaact ccagcctaac tggagcggtg caatggaaac tattaagcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggtc aaatgcgtag atctcgggag
      181 gaacaccgat ggcgaaggca gcttcttggc tcattcctga cgttgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004990
VERSION     KHUT01004990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           93 g           49 t
ORIGIN      
        1 tacgaggggt gcgagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtttcg
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg cctcggaaac ggcgaaactc
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagttactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004991
VERSION     KHUT01004991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaatgg cattggaaac tggcttacta
      121 gagtgcggta gagggatgcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatctgt ggcgaaggcg gcatcctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004992
VERSION     KHUT01004992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggttttt
       61 caagtccgac gtgaaatctt ctggcttaac caggagcggt cgtgggaaac tgagagactc
      121 gaggcgatga gaggggtgca gaattccagg tgtagtggtg gaatgcgtag atatctggag
      181 gaataccagt ggcgaaagcg gcaccctggc atcgaactga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01004993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004993
VERSION     KHUT01004993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggcgctg
       61 aaagtcttct gtgaaatcta cgggcttcac ccgtaacgtg cagaggatac tacagtgcta
      121 gagtgtcgga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01004994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004994
VERSION     KHUT01004994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccga
       61 taagtctgct gtgaaagtcc agggctcaac cctggaatgc cggtggatac tgtcgggcta
      121 gagttcggaa gaggcaagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gcttgctggg acgacactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01004995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004995
VERSION     KHUT01004995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           89 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgctg
       61 taagtcaggt gtgaaatttc ggagctcaac tccgaaactg cacttgatac tgcggtgctt
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01004996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004996
VERSION     KHUT01004996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           84 g           56 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggactta
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg cacttgatac tgcaagtctt
      121 gagtgtggta gaggctatta gaattcttgg tgtagtggtg aaatacgtag atatcaagag
      181 gaataccgga ggcgaaggcg gatagctggg ccaacactga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01004997             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004997
VERSION     KHUT01004997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          101 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccttt
       61 taagtccatc ggtgaaatgc cggggctcaa ccccgagctt gccgaggata ctgggaggct
      121 ggagacacgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004998             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004998
VERSION     KHUT01004998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctttt
       61 taagtccgtt ggtgaaatgc cggggctcaa ccccgtgccc gccagggata ctggagagct
      121 ggagacgagt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gctcggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01004999             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01004999
VERSION     KHUT01004999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           48 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggctcgta ggcggttcga
       61 caagtcgggt gtgaaacctc caggctcaac ctggagtcgc cgctcgaaac tgttgtgact
      121 agagtccggt agaggagcat ggaatttgtg gtgtagcggt ggaatgcgca gatatcacaa
      181 ggaacaccag tagcgaaggc gatgctctgg gccggtactg acgctgagga gcgaaagcat
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005000
VERSION     KHUT01005000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggcttcg
       61 taagtcttgt gtgaaagccc tcggctcaac cgaggaattg cacgagaaac tgcgtggctc
      121 gagtccggga gaggatagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctatctgga ccggtactga cgctgatacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005001
VERSION     KHUT01005001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           75 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggccaga
       61 taagtcagtg gtgaaatcct gttgcttaac aacagaactg ccattgatac tgtttggctt
      121 gagtatagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctataactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01005002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005002
VERSION     KHUT01005002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattaa ctgggcgtaa agggcgcgta ggtggttata
       61 agagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gatataactg
      121 gagtattgga gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gctacctggc cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005003
VERSION     KHUT01005003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctga
       61 taagcgggga gtgaaagccc acggcttaac cgtggaagtg ctttccgaac tgtcaggctt
      121 gagtacgcaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gcgatctggg gcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005004
VERSION     KHUT01005004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          102 g           40 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggtggacgcc
       61 caagcggggt gtgaaagctc ctggcttaac tgggagagtg cgctccgaac tgggcgacta
      121 gagggcaggc gacggaggtg gaatacccgg tggagtggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gccttcgagc ctgtgcctga cactgaagcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005005
VERSION     KHUT01005005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctctt
       61 caagtcgggg gtgaaatccc acagcttaac tgtggaagtg ccttcgatac tgaggagctc
      121 gagcgaggta ggggccgagg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg ctcggctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005006
VERSION     KHUT01005006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaactg cgtcttatac tgcatagctt
      121 gagtacatga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc atgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005007
VERSION     KHUT01005007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggttcg
       61 caagtaggat gtgaaagccc caggcttaac ctgggaatgg caccctaaac tacgaatctt
      121 gaatgcttga gaggttggtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaatatcgga ggcgaaggcg accaactggt ggtgcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005008
VERSION     KHUT01005008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcctt
       61 taagtcagtg gtgaaatcct ccgactcaat cggagaactg ccattgatac tattggtctt
      121 gaatacagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005009
VERSION     KHUT01005009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttgat
       61 taagttatct gtgaaatccc tgggcttaac ctgggcaggt cagatgatac tggttgactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005010
VERSION     KHUT01005010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           79 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtctg
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tataggtctt
      121 gaataccgat gaggtgggcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaaa cggtcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005011
VERSION     KHUT01005011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           85 g           46 t
ORIGIN      
        1 gacgagccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcga
       61 aaagttgggt gtgaaatacc tcggctcaac cgaggaactg cgcccaaaac tatcgtgctc
      121 gaggaagata gaggtgagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacacccgt ggcgaaagcg gctcactggg tcttatctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005012
VERSION     KHUT01005012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           89 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcaaag
       61 caagtctgaa gtgaaagccc cgggctcaac ccgggaactg ctttggaaac tgtttagcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005013
VERSION     KHUT01005013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agagtgcgta ggcggcgcgg
       61 aaagtcagat gtgaaatccc ggggctcaac ctcggaacgg catccgatac tcccgcgctt
      121 gaggactgga ggggagtctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg agactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcgaag ggg
//
LOCUS       KHUT01005014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005014
VERSION     KHUT01005014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           39 c           89 g           59 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatga ctgggcgtaa agggagcgta ggcggttgag
       61 tacgtttggt gtgaaagtcc tgggcttaac ctggggactg cattgaatac ggcttgactt
      121 gaggtataga ggggaagatg gaattgtgtg tgtagaggtg aaattcgtag atatacacaa
      181 gaacaccagt ggcgaaggcg atcttctggc tattacctga cgctaaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005015
VERSION     KHUT01005015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           98 g           42 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcgggtcaa
       61 caagtcatgc gtgaaagctc ctggctaaac tgggagaggt cggatgagac tgttggtctg
      121 gagggcgtca gaggagtgtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gcactctggg gcgcacctga cgctgaggcg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005016
VERSION     KHUT01005016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           82 g           55 t
ORIGIN      
        1 tacgagggga gctagcgtta ttcggaatta ctgggcgtaa agcgcgcgta ggcggttttt
       61 taagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgaaac tggaaaactt
      121 gaatcttaga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactggc taagcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005017             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005017
VERSION     KHUT01005017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           81 g           59 t
ORIGIN      
        1 tacagaggtc acaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggtgggtta
       61 gtaagttacc cttcaaagac cacggctcaa ccgggggaag gggggtaaga ctattagtct
      121 tgatatttgg tggggctttc ggaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaactccaa gggcgaaggc aggaagctaa ccaatttatg acactgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01005018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005018
VERSION     KHUT01005018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatta ctgggcttaa agggtgcgta ggcggactcg
       61 caagtgcttt gtgaaatccc acggctcaac catggaattg cttggcagac tgcgggtctt
      121 gaggttggta ggggtcgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccaaatctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005019
VERSION     KHUT01005019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           96 g           38 t
ORIGIN      
        1 gacggagggc gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggactg
       61 taagtccgag gtgaaagccc ggggctcaac cccgggactg ccttggaaac tgcagtcctc
      121 gagtgcagga gaggaaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005020
VERSION     KHUT01005020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c          103 g           39 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctga
       61 gaagttctgg gtgaaagccc ccggcttaac cggggaaggg cctggaaaac cattgggctg
      121 gagtgctgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacatcggt ggcgaaggcg gcttcctgga cagacactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01005021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005021
VERSION     KHUT01005021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           88 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggtgggatat
       61 taagtctggt gttaaagttc ggggctcaac cccgtgaagc atttggaaac tgatattctt
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtgtctga cactgaggcg cgaaagccag
      241 ggtagtgaac ggg
//
LOCUS       KHUT01005022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005022
VERSION     KHUT01005022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcgcag
       61 taagtctgat gtgaaatctc ccggcttaac tgggaggggt cattggaaac tgcagtgctt
      121 gaggcggtga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc actagcctga cgctcatgtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005023
VERSION     KHUT01005023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgc
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggatac tggcggactt
      121 gagggaggca ggggttgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacaactggt cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005024
VERSION     KHUT01005024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           42 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcggtt
       61 caagtcgggc gtgaaaggcc tgggctcaac ccgggaatgg cgctcgagac tggactgctc
      121 gagtccggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005025
VERSION     KHUT01005025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacagggggc tcgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggctcat
       61 caagtgtttt gtgaaatccc cgagctcaac tcgggaacag cagggcaaac tggtgagctt
      121 gaggcaggta ggggccgaca gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggggaagccg gtcggctggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005026
VERSION     KHUT01005026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccctc
       61 taagtcgaac gtgaaatccc tgggctcaac ccaggaactg cgtccgagac tggggggcta
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005027
VERSION     KHUT01005027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggttagg
       61 taggtccggt gtgaaaactc gaggcttaac ctcgagaggt cgccggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaagca gctctctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005028
VERSION     KHUT01005028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           92 g           43 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggattgg
       61 caagacagat gtgaaagccc cgggctcaac ctgggaacgg catttgtgac tgcgagtcta
      121 gagtatggca gagggaggtg gaattccatg tgtagcagtg aaatgcgtag agatatggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005029
VERSION     KHUT01005029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           94 g           41 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccggg
       61 caagtcggat gtgaaatccc gaggctcaac ctcggaactg cattcgaaac tgctcggctc
      121 gagtcccgga gaggatggca gaattcccgg tgtaggggtg aaatccgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005030
VERSION     KHUT01005030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 caagtccggg gtgaaatcct ccagctcaac tggagaacgg ccccggatac tggcggtctc
      121 gaggagggta ggggcaagcg gaactatggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaggcg gcttgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005031
VERSION     KHUT01005031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggttgc
       61 caagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccgtggatac tggtaatctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttgctaca cgattattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005032             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005032
VERSION     KHUT01005032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           49 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatta ctgggcataa agcgcgcgta ggcggcgacg
       61 taagtgtcgg gtgaaatccc acggctcacc cgtgtgaatt gctgggcata ctgcgttgct
      121 cgagtgtggt aggggagagc ggaactcctg gtggagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggctctctgg tccactactg acgctgaagt gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01005033             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005033
VERSION     KHUT01005033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           43 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcgggt gtgaaaaccc caggctcaac ctggggacgc cacccgagac tgcgatgact
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cggcgaaggc agcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01005034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005034
VERSION     KHUT01005034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           83 g           57 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtgggctta
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg catttgatac tgtaagcctt
      121 gaatgtggta gaggttacta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccaga ggcgaaggcg ggtaactgga ccaacattga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005035
VERSION     KHUT01005035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          103 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaagtt
       61 taagcggtgc ttgaaagccc ccggctcaac cggggagagt ggtgccgaac tggacttctt
      121 gagggcgata gaggagagtg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaggcg gctctctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005036
VERSION     KHUT01005036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtgggttgg
       61 caagttcgat gtgaaagccc cgggcttaac ccgggaaagg cattgaaaac tgctgatctg
      121 gaggattgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caatacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005037             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005037
VERSION     KHUT01005037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           49 t
ORIGIN      
        1 tacggggggt gcaagcgtta ttcggattta ttgggcgtaa agggcgcgta ggcggtctgg
       61 taagtctggt gtgaaaacgc taccgctcaa cggagcgatt gcactggata ctgctagact
      121 tgagtgcggg agaggtgagc ggaatgtgtg gtgtagcggt ggaatgcgta gatatcacac
      181 agaatacccg tggcgaaggc ggctcactgg cccgcaactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01005038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005038
VERSION     KHUT01005038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           85 g           56 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtgcgt
       61 caagtcagtt gtgaaatctt aaggcttaac cttaaacggt caactgatac tgtcgtgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgcttgg ctgacactga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005039
VERSION     KHUT01005039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagaacgta ggcggccact
       61 taagtttggt cttaaagact ggggctcaac ctcaggaatg gatcgaatac tgggtggcta
      121 gaggacgtca gaggaaggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg accttctggg acgttcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01005040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005040
VERSION     KHUT01005040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggatggg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgcttgtctt
      121 gaggcactca ggggcagctg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaaa ggtgaaaaca ggctgctggg agtgatctga cactgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005041             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005041
VERSION     KHUT01005041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           94 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgggtcgtgg gtgaaatccc ccagcttaac tggggaactg cccccgatac cactgcggct
      121 ggaggaagga aggggcattt ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgccga tggcgaaagc agaatgctgg tcctttactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01005042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005042
VERSION     KHUT01005042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           82 g           60 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttt
       61 taagtcagat gtgaaatccc tgggctcaac ctaggaattg catttgatac tgaataactt
      121 gagtatggta gagggaagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccattactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005043
VERSION     KHUT01005043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           90 g           50 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtaga gtgaaatccc tgggctcacc tcaggaaccg ctctgcatac tggcagactg
      121 gagggtcgga gaggtatgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcatactggc cggcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005044
VERSION     KHUT01005044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggttgttgca
       61 taagttggtt gtgaaagccc tgggcttaac ctgggaattg caaccaaaac tgtgtaacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005045
VERSION     KHUT01005045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           92 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtcttt
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tgggaggctt
      121 gaggatggga gaggcatgcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gcatgctgga ccatttctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005046
VERSION     KHUT01005046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttagt
       61 taagatagct gtgaaatccc tgggcttaac ctaggaactg cagctgtgac tggctagcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005047
VERSION     KHUT01005047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgc
       61 tgtgtccgat gtgaaagccc cgggcttaac ctgggaatgg cattggaaac tggcgggctt
      121 gagtgcggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg tcgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005048
VERSION     KHUT01005048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           85 g           48 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagtgc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tggttctctt
      121 gagccaccta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctgga agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005049
VERSION     KHUT01005049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           93 g           45 t
ORIGIN      
        1 gacgaaccgc ccgaacgttg ttcggaatca ctgggcttaa agggagcgta ggcgggctgc
       61 caagtccggg gtgaaatcct ccagcttaac tggagaactg cctcggatac tggaagtctc
      121 gaggagggta ggggcaagtg gaaccgtggg tggagcggtg aaatgcgttg atatccatgg
      181 gaactccggt ggcgaaggcg acttgctgga ccctttctga cgctgaggct cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005050
VERSION     KHUT01005050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcatgg
       61 caagtaaggt gtgaaatctc ctggctcaac cgggagggtg cattttagac tgccaagctt
      121 gaggtcggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005051
VERSION     KHUT01005051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           97 g           41 t
ORIGIN      
        1 tacggagagt gcaaacgttg ttcggattta ctgggcgtaa agggcacgta ggcggctcgg
       61 caaatcggtc gtggaatccc ggcgcttaac gtcggaacgg cggccgaaaa taccgggctc
      121 gagggcgtga ggggtcactg gaactcctgg tgaagcggtg aaatgcgtag atatcaggag
      181 gaacgccgga ggcgaaagcg agtgactggc gcgttcctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005052
VERSION     KHUT01005052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           41 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggaccgg
       61 aaagcgtcct gtgaaatccc acggctcaac cgtggaactg cagggcgaac tgccggtctt
      121 gaggaaggta ggcgccgacg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg gtcggctggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005053
VERSION     KHUT01005053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           89 g           46 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaatgg cgctctaaac taccgtgctt
      121 gagggagaca gaggtaagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttctctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005054
VERSION     KHUT01005054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccata
       61 caggtctgct gtgaaaactc gaggctcaac ctcgagctgt cggtggaaac cgtatggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggctaaggcg gctctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005055
VERSION     KHUT01005055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           85 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctaga
       61 aaagttggat gtgaaatccc tcggcttaac caaggaagtg catccaaaac tgcctagctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005056
VERSION     KHUT01005056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 cacgtagggg gcgagcgtta tccggattta ctgggcgtaa agcgcgttaa ggcggtcggc
       61 aaagttgggg gtgaaattcc agggctcaac cctggaactg cctccaatac tggtcgactt
      121 gagtgcgaga gaggaaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggc tcgcaactga cgctgtagcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005057
VERSION     KHUT01005057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           80 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctaat
       61 taagtcagcg gtgaaattcc acagctcaac tgtggaactg ccattgatac tggttggctt
      121 gagtacagat gaggtaggcg gaattgacag tgtagcggtg aaatgcttag atattgtcaa
      181 gaacacctat tgcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005058
VERSION     KHUT01005058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agagcgcgca ggcggtgcgt
       61 taagtccgag gtgaaatctc ccggctcaac tgggagcggt cttcggatac tagcggactt
      121 gaggagagca gaggtgagtg gaattctcgg tgtagtggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg actcactggg ctccacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005059
VERSION     KHUT01005059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggtcgt
       61 ttagttagag gtgaaagccc agggctcaac cctggaattg cctttaatac tggcggcctt
      121 gagtatggca gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005060             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005060
VERSION     KHUT01005060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           38 c           75 g           58 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgtgta ggtggtagag
       61 caagttatta gttaaagtcg agctcaactc aacgaaggct ggtaaaactg ttctgctaga
      121 ggaaagaaga ggttgacgga aatctatgtg taggggtgaa atccattgat ctatagaaga
      181 ccaccaaaaa aggcgaaggc agttaactat tctattcctg acactgaggc atgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01005061             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005061
VERSION     KHUT01005061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           89 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtccgta ggcggcacgt
       61 taagtgagat gcgaaatctg gcggctcaac cgtctagatg ttgtctcata ctggcgagct
      121 ggaggatggt agaggcaagc ggaattaccg gtgtagcagt gaaatgcgta gatatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatttctg acgctgaggg acgaaagcgt
      241 ggggagcgaa ctgg
//
LOCUS       KHUT01005062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005062
VERSION     KHUT01005062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgt
       61 taagtgaggg gtgaaaggtt cgggctcaac ccggacactg cctttcatac tggcgacctt
      121 gagtttggga gaggcgagtg gaatggtcgg tgtagcggtg aaatgcgtag atatcgatcg
      181 gaacacccat ggcgaaggca gctcgctggc ctaaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005063
VERSION     KHUT01005063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtgat
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaaatg cattcgaaac tggtcgccta
      121 gagtgttgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga caattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005064             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005064
VERSION     KHUT01005064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           47 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggacctg
       61 taagtctgac gtgaaatctc caccgctcac cggggagagg ccgttggata ctgcagatct
      121 ggagttcggt agaggatcgt ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg tggcgaaggc ggcgatctgg gcctgaactg acgctgagga gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005065
VERSION     KHUT01005065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          104 g           39 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgtgcgca ggcggctttt
       61 caagcgccac gtgaaagccc cgggctcaac ccgggagggt cgtggcgaac tgggaggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtactga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005066
VERSION     KHUT01005066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          102 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgtgca ggcggctttg
       61 taagtcccgt gtgaaagcct acggctcaac tgtaggaggc cgcgggatac tgcgaggcta
      121 gagggcagga gagggaagta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatgccagt ggcgaaggcg gcttcctggc ctgtccctga cgctgagacg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005067
VERSION     KHUT01005067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 gacgtaggag gcaagcgttg ttcggaatta ctgggcgtaa agcgtccgta ggcgggtcgg
       61 taagtggata gttaaaaccc ggggctcaac cccgagggtg ctgtccggac tgccggtctg
      121 gaggtcggca gagggtaatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgtaagcg attacctggg ctagacctga cgctgaggga cgaaggcgtg
      241 ggaagcaaac ggg
//
LOCUS       KHUT01005068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005068
VERSION     KHUT01005068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c          103 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtct
       61 caagtccggg gtgaaagcct cccgcccaac gggagaagcg cctcggagac gggggccctc
      121 gaggggggta ggggcgagcg gaactgtggg tggagcggtg aaatgcattg agatccacag
      181 gaactccggt ggcgaaggcg gctcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005069
VERSION     KHUT01005069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c          100 g           47 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggattga ctgggcgtaa agggcgtgta ggcggttggt
       61 caagtctgat gtgaaagccc gaagcttagc ttcggaagtg cgtcggaaac tagccgactg
      121 gagcgcgata gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccctctgga tcgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005070
VERSION     KHUT01005070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggattta ctgggcgtaa agagcgcgta ggcggttcga
       61 ttagtcggag gtgaaagccc tcggctcaac cgaggatccg cgtccgagac tgtcgaactt
      121 gagtgcagga gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ctgctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005071
VERSION     KHUT01005071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           87 g           55 t
ORIGIN      
        1 taccagctct ccgagtggtg tggatgttta ttgggcctaa agcatccgta gctggctaag
       61 ttagtccctt gttaaatcca ccgaattaat cgttggattg cgggggatac tgcttggcta
      121 ggggacgaga gaggcagacg gtatttccgg ggtaggggtg aaatcctata atcccgggaa
      181 gaccaccagt ggcgaaggct gtctgctaga acgcgcccga cggtgaggga tgaaagctgg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005072
VERSION     KHUT01005072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           88 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggactga
       61 taagtcaggt gtgaaatccc tgggctcaac ccaggaactg cacttgaaac tgccagtctt
      121 gagtgatgga gaggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccatctgga aatctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005073
VERSION     KHUT01005073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtcttc
       61 caagtcctgt gtgaaagccc tgggctcaac ccaggaagtg cgcaggatac tggttgactg
      121 gagtggcgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgttcactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005074
VERSION     KHUT01005074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtgatt
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaacgg catttgatac tgggtcactt
      121 gagtgtggta gaggagagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctctctgga ccaacactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005075
VERSION     KHUT01005075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggtttgc
       61 caagtccggc gtgaaatctc ccggctcaac tgggagggtg cgccggatac tggtaggcta
      121 gagtgcggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctctctgga ccgccactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005076
VERSION     KHUT01005076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatgtag
       61 caagtctgct gtgaaagccc ccggcttaac cggggaagtg cagaagatac tgcttcattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005077
VERSION     KHUT01005077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcttgta ggtggccaac
       61 taagtccgac gtgaaatccc taggcttaac ctaggaactg cgtcggatac tggatggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cactgaaaag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005078             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005078
VERSION     KHUT01005078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          108 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 taagcgggcg gtgaaagccc ggggcttaac cccgggtctg ccgtgcggac tgccgtggct
      121 tgagcactgt agaggcaggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcctgctgg gcagtggctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005079
VERSION     KHUT01005079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           98 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctatc
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaactg cgtttgggac tggatggcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005080
VERSION     KHUT01005080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggcttgt
       61 cgcgtcgatc gtgaaaactc ggggctcaac cccgagcttg cggtcgatac gggcaggctt
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgagaag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005081             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005081
VERSION     KHUT01005081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 cgtgtccgat gtgaaagccc cgggcttaac ctgggaattg cattggaaac tgacgggctt
      121 gagtgcggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgacactga cgctgagggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005082
VERSION     KHUT01005082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta gtcggtattg
       61 agagtcacgg gtgaaatccc aaggcttaac cttggaactg cctgtgagac ctcagtacta
      121 gagtgtgaga ggggatagtg gaatacccag tgtagcggtg aaatgcgtag agattgggtg
      181 gaacaccggt ggcgaaggcg gctatctggc tcacaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005083
VERSION     KHUT01005083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggcttc
       61 taagttagat gtttaatccc ggggcccaac cccggacagg cacctaaaac tggaagtctt
      121 gaatagtgga gaggacggcg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccagt tgcgaaggcg gccgtctgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005084
VERSION     KHUT01005084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcactg
       61 ttggtcggat gtgaaatccc gcggctcaac cgtggaagtg cattcgatac cgcagagcta
      121 gagtatcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgattactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005085
VERSION     KHUT01005085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           85 g           49 t
ORIGIN      
        1 gacgaacctt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcagtgcag
       61 aaagtcgggt gtgaaatccc tcagctcaac tgaggaattg cgcccgaaac tgctgtgcta
      121 gaggaataca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tattttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005086
VERSION     KHUT01005086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggattca ttgggcgtaa agggtgcgta gacggcgtgt
       61 taagtttgtt gttaaaggct ctggcctaac cggagtattg caatgaaaac tggcatgcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005087
VERSION     KHUT01005087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtcaag
       61 taagtgagat gtgaaagccc tgggcttaac ctaggaactg catcttatac tgtttgactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005088
VERSION     KHUT01005088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           98 g           48 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ttgggcgtaa agcgcccgca ggcggcttat
       61 caagtctgac gtgaaatcgc tcagcttaac tgggcgcggt cgttggaaac tgatgggctt
      121 gaggcaagga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacatcagt ggcgcaagcg gcaccctggc cttggcctga cgctcagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005089
VERSION     KHUT01005089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c          101 g           37 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctcgg
       61 tcagtccgct ctgaaagccc ggggctcaac cccgggaggc gggcggatac tgccgggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005090
VERSION     KHUT01005090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcactc
       61 taagtacgct gtgaaatatc ccggctcaac cgggagggtg cagtgtagac tagagagctt
      121 gaggtcggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005091
VERSION     KHUT01005091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggactgc
       61 caagtggtgt atgaaatcgc tcggctcaac cgggcgcggt tatgccagac tggcagacta
      121 gagactgaca gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gccacctggg tcagttctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005092
VERSION     KHUT01005092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           94 g           50 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccggg
       61 taagtctgac gtgaaatccc ttggctcaac caaggaattg cgtcttatac tgtctggctt
      121 gagtccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01005093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005093
VERSION     KHUT01005093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           98 g           37 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgca ggtggctccg
       61 cacgtccggt gtgaaagccc ggggctcaac cccggaagcg cgctggatac gacggagctt
      121 gagggctgga gaggcaggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcctgctgga cagcacctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005094
VERSION     KHUT01005094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          104 g           36 t
ORIGIN      
        1 cacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgtgcgca ggcggtctgg
       61 tcagtcgatg gtgaaagccc cacgctcaac gtgggaaggt ccgtcgagac ggccagactg
      121 gaggcaggga gaggccggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gccggctgga cctgacctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005095
VERSION     KHUT01005095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcgcg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgcgtggcta
      121 gggtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttaccga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005096
VERSION     KHUT01005096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggggtat
       61 caagtggtgt atgaaagggc gcggctcaac cgcgctaggt tatgccagac tgatgctctt
      121 gaggaccaaa cagggctgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcagcctggt tggtatctga cgctcggaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005097
VERSION     KHUT01005097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgccccg
       61 caagtcggat gtgaaagccc tgggctcaac ctgggaagtg cattcgaaac tgcggggctg
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005098
VERSION     KHUT01005098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctctt
       61 caagttgggc gtgaaagctc caggcttaac ctggaaattg cgtttgatac tgtcgagcta
      121 gagtgtggaa gaggctacta gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaataccagc ggcgaaggcg agtagctggg ccaacactga cgctgaggtg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005099
VERSION     KHUT01005099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcctgc
       61 caagtccggg gtgaaagcct ccagcttaac tggagaagtg ccctggatac tggcgggctc
      121 gagcgaggca ggggtcgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacgactggt cctctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005100
VERSION     KHUT01005100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          110 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggggacg
       61 ggcgtcggtg ctgaaagggg tccgcttaac ggaccgatgg gtgccgatac gccgtttctg
      121 gagcgcgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt ggcgcaagcg gccacctagc ccgcggctga cgctgaggcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005101
VERSION     KHUT01005101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgt
       61 caagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tagcgagctt
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005102
VERSION     KHUT01005102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           87 g           48 t
ORIGIN      
        1 tacgagaggt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtccag
       61 aaagtggggt gtgaaagccc tcggcttaac cgaggaactg cgctccaaac tactgggcta
      121 gaggaagata gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005103
VERSION     KHUT01005103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           60 c           96 g           38 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtcgtg
       61 cacgtggggt gtgaaaactg gcggctcaac tgccagaggc cacctcatac ggcgcgactc
      121 gagccatgca gaggtccatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg catgtgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005104
VERSION     KHUT01005104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           63 c           99 g           34 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggagtta ctgggcgtaa agcgcgtgca ggcggcgccg
       61 cacgtcgggt gtgaaagccc ggggctcaac cccggaagcg catccgatac gacggcgctc
      121 gaggacggga gaggagcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgcaggcg gcgctctgga ccgtcactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005105
VERSION     KHUT01005105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcgcgta ggcgggtcgt
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tggcgatctt
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005106
VERSION     KHUT01005106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           75 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattag
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaactg ccattgatac tgctagtctt
      121 gaatatagtt gaggtaggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gcttgctaaa ctattattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005107             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005107
VERSION     KHUT01005107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           97 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggccgag
       61 taagtctcac tgtgaaagtc cggggctcaa cctcgggatt gcaggggata ctgcacggct
      121 tgagacaagt agaggttggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggccaactgg gcttgttctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005108             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005108
VERSION     KHUT01005108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          101 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggccggg
       61 ccagtctatg ggtgaaaggc cggggctcaa ccccgtgtgt gcccaggata ctgtccggct
      121 ggagacacgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005109             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005109
VERSION     KHUT01005109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           49 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaactt tgggctcaac ccaaagcgtg cactcgatac tgccatgact
      121 agagttcggt aggggagtgg ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005110             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005110
VERSION     KHUT01005110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           50 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggtgtaa agggtgcgta ggctgttcgt
       61 caagtctgat gtgaaagccc tgggcttaac tcaggaattg cattggaaac tgacggactc
      121 agagtatcgg agagggaagc ggaattcctg gtgtagcggt gaaatgcgta gataccagga
      181 ggaacaccgg tggcgaaagc ggtttcctgg ccgaatactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01005111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005111
VERSION     KHUT01005111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcagtg
       61 taagttgggc gtgaaatcct ggggcttaac ctcagaattg cgttcaatac tgcattgcta
      121 gggcgctgta gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg aatctctggg cagcagccga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005112             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005112
VERSION     KHUT01005112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           86 g           46 t
ORIGIN      
        1 gacgagagtg gcaagcgtta ttcggaatta ttgggcgtaa agcgtccgta gggggatctt
       61 cacgtcgggt gtaaaagcca gaggctcaac ctctgcgaca gcatccgata cggaggatct
      121 agaggactgt agaggcatat ggaattccag gagtaggggt aaaatccgta gatacctgga
      181 ggaacaccga tagcgaaggc aatatgctgg gcagctcctg accctgaggg acgaaagcgt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01005113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005113
VERSION     KHUT01005113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggctgt
       61 taagtggtgt atgaaatatc ccggctcaac cgggaagggt tatgccagac tggcagtcta
      121 gagagcgaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcatctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005114
VERSION     KHUT01005114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtcggtg gtgaaatcct ccagcctaac tggagaagag ctgtcgatac tgggtgcctc
      121 gaggggtgca ggggtctgcg gaacttcggg tggagcggtg aaatgcgtag agatccgaag
      181 gaacgccggt ggcgaaagcg gcggactggg caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005115
VERSION     KHUT01005115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggcttct
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctctgatac tgaggagcta
      121 gagtccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005116
VERSION     KHUT01005116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          103 g           37 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggcgccg
       61 cgcgtggggt gtgaaagcgg gcggcttaac tgcccaaggt catcccatac ggcggtgctg
      121 gagccatcca gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgactggg gatgtgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005117
VERSION     KHUT01005117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggctgatttg
       61 taagtcagtg gtgaaatctc acagctcaac tgtgaaactg ccattgatac tgcaagtctt
      121 gagtgttgtt gaagtagctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggttactaag caacaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005118
VERSION     KHUT01005118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           77 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggcttgt
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cccttgatac tgatgagctt
      121 gaattaacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactatg gttatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005119
VERSION     KHUT01005119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           49 t
ORIGIN      
        1 tacgtagggt ccgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagccagat gtgaaatccc cgggctcaac ctgggaactg catttgggac tgcaaggctt
      121 gagtacggca gaggggggta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca gccccctggg tcgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005120
VERSION     KHUT01005120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaattg cctttgaaac tgtcaggctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggaactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005121
VERSION     KHUT01005121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgtacgta ggcggtcctt
       61 taagtcgggt gtgaaagccc aaggctcaac cttggaagtg cattcgaaac tgaagggctt
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005122
VERSION     KHUT01005122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           98 g           45 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgcgg
       61 caagtcaggt gtgaaagccc cgggctcaac tcgggaactg catctgaaac tgccatgctt
      121 gagtcctgga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgt ggcgaaggcg gctacctgga cagtgactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005123
VERSION     KHUT01005123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggtggttctg
       61 tatgtccacc gtgaaagcgt acggctcaac cgtatttagt cggtggaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccccg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005124
VERSION     KHUT01005124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtcggtg gtgaaatcct ccggctcaac cggagaagag ctgtcgatac tggatgcctc
      121 gaggggtgta ggggcctgtg gaactttggg tggagcggtg aaatgcgtag agatccaaag
      181 gaacgccggt ggcgaaagcg acgggctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005125
VERSION     KHUT01005125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          100 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgtc
       61 cgagtccggg gtgaaatctt tcggcctaac cggaaaaggg ccctggatac tggacggctc
      121 gaggggggta ggggcaagcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gcttgctgga cccctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005126
VERSION     KHUT01005126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggctttt
       61 caagtcaggg gtgaaatccg gcagctcaac tgtcgaagtg cccttgatac tgatgagctc
      121 gagggaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005127
VERSION     KHUT01005127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           88 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggccgttcac
       61 taagttcgat gtgaaatccc ccggctcaac cggggaactg catcgaatac tggtggactc
      121 gagagttgta ggggtggacg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccgga ggcgaaagcg gtccactggg caacttctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005128             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005128
VERSION     KHUT01005128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           48 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcaggt gtgaaacctc caggcttaac ctggagacgc cacctgatac tgctgtggct
      121 ggagtccggt aggggagcag ggaatatccg gtgtagcggt gaaatgctta gatatcgggt
      181 ggaacaccag tggcgaaggc gctgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005129
VERSION     KHUT01005129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcaga
       61 taagtctgat gtgaaagctc tgggctcaac ccagaaatgg cattggatac tatttggctt
      121 gaggaatgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga catttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005130
VERSION     KHUT01005130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggactct
       61 taagtcgggg gtgaaagccc agggctcaac cctggaattg ccttcgatac tgggagtctt
      121 gagtatggca gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggg ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005131
VERSION     KHUT01005131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           50 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagtccgta ggcggcgcag
       61 atagtggggt gtgaaatccc tcggcttaac cgaggaattg cgctccaaac tcctgtgctt
      121 gaggaatcta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg gattttctga cgctgaggga cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005132
VERSION     KHUT01005132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           46 c           98 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtgtcc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggggtgctg
      121 gagtgtggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005133
VERSION     KHUT01005133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          103 g           44 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcttg
       61 taagtttgtg gtgaaagccc ggggctcaac cccgggtctg ccgcgaatac tgcgagactt
      121 gagtactgta gaggctggta gaatgccggg tgtagcggtg gaatgcgtag agatccggca
      181 gaagaccggt ggcgaaggcg gccagctggg cagtgactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005134
VERSION     KHUT01005134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggctatg
       61 taagttggct gtgaaagctc ccggcttaac tgggagaggt cagtcaaaac tgtgtagcta
      121 gaggctggaa gaggcgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgcaagcg gctcgctggt tcagccctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005135
VERSION     KHUT01005135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           76 g           61 t
ORIGIN      
        1 gacgaaggat tcaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggaactc
       61 taagtcagtg gtgaaatccc gcagcttaac tgcggaactg ccattgaaac tggttttctt
      121 gaatacagtg gaggtagatg gaatataaca tgtagcggtg aaatgcttag atatgttata
      181 gaacaccgat tgcgaaggca gtctgctaca ctgtcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005136
VERSION     KHUT01005136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggattta ctgggcgtaa agcgcgtgta ggcgggcgac
       61 taagtcggat gtgaaatccc tgggctcaac ctaggaactg cattcgaaac tggtcaccta
      121 gagtgatgga gaggaaagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga catttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005137
VERSION     KHUT01005137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtatt
       61 caagtctggg gtgaaatcct ctcgcttaac gagagaacag ccctggatac tgggtacctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005138
VERSION     KHUT01005138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccgc
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaagtg cctttgatac tggcggactc
      121 gagggaggta ggggcagatg gaactggtgg tggagcggtg aaatgtgtag atatcactag
      181 gaacgccggt ggcgaaagcg atctgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005139
VERSION     KHUT01005139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgatac tggcaagctt
      121 gagggaggta ggggcctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgggctggc cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005140
VERSION     KHUT01005140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggatgg
       61 caagtcggat gtgaaagccc tcggctcaac cggggaagtg cattcgaaac tgctgttctt
      121 gagtgtcgga gagggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg accacctgga cgaacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005141
VERSION     KHUT01005141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtcggac gtgaaaggct ggggctcaac cccagatcgc cgttcgaaac tgccgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cgacatctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005142
VERSION     KHUT01005142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           88 g           45 t
ORIGIN      
        1 gacgaaggtg gcaaccgttg ctcggaatca ctgggcataa agcgcacgta ggtggctaag
       61 taagtcggtg gtgaaatacc ttggctcaac caaggaagtg ccatcgatac tgccatgctt
      121 gaggacggga ggggtgagtg gaactcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg actcactgga ccgatcctga cactgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005143
VERSION     KHUT01005143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           86 g           47 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccatg
       61 tgtgtgaggt gtgaaatccc ggagcttaac tccggaactg cgcctcaaac tacatggcta
      121 gagcattgga gagggaagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg acttcctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01005144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005144
VERSION     KHUT01005144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           38 c           83 g           64 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ctgggtttaa agggtacgta ggtgggcatc
       61 taagtctgga gtgaaagttt gaggctcaac ctcgaaagtg ctttggatac tggttgtcta
      121 gaattagaga gaggtagctg gaatttgtgg tgtagcggtg aaatgcatag ataccacgag
      181 gaacaccgat tgcgaaggca ggctactggc tctatattga cactgatgta cgaaagtgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005145
VERSION     KHUT01005145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agggcgcgta ggcggcccgg
       61 taagtccgct gtaaaagtca aaggcttaac ctttgaatgc cggtggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat agcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005146
VERSION     KHUT01005146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           96 g           40 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcaggg gtgaaagccc acggctcaac cgtggaagtg cctttgatac tggtcgtctc
      121 gagggggata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005147
VERSION     KHUT01005147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggatgtt
       61 taagttgggg gtgaaagccc agggctcaac cctggaactg ccttcaatac tggacatcta
      121 gagtatggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005148
VERSION     KHUT01005148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c          102 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agggtgcgca ggcggtcggg
       61 tatgtgccga gtgacaaatc cgcgcttaac gcggaagggt ctcggtagac agccagacta
      121 gagtccttga gagggaagtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagg ggcgcaggcg gcttcctggc aaggcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005149
VERSION     KHUT01005149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           93 g           56 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggttttc
       61 taagtctggt gtgaaatctc ctggcctaac tgggagggtg cgctagaaac tggaaggctt
      121 gagtatggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccatcactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005150
VERSION     KHUT01005150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccgcg
       61 acagtcggat gtgaaagccc tgggctcaac tcaggaagtg cacccgatac tacgtggctc
      121 gagtaccgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg gccaactgga cgagtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005151
VERSION     KHUT01005151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           64 c          102 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcga
       61 aaagtccgtg gtgaaatccc cccgctcaac gggggaacgg ccgcggatac tgtcggcctc
      121 gaggggggta ggggcgagcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gctcgctgga cccccactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005152
VERSION     KHUT01005152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggacagg
       61 taagtccgtg gtgaaatctc tgagcttaac tcagaaactg ccatggatac tatttgtctt
      121 gaatactgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacatg
      181 gaacaccaat tgcgaaggca gcttactaca cagctattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005153
VERSION     KHUT01005153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcgggcgaa
       61 taagtcaggg gtgaaatgcc aaggctcaac cttggaactg cctttgatac tgttcgtctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005154             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005154
VERSION     KHUT01005154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           94 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcggat gtgaaatctc caggctcaac ttggaggggt cattcgatac tgccatgact
      121 agagttcggt aggggaatgt ggaatacctg gtggagcggt gaaatgcgca gatatcaggt
      181 ggaacacccg tagcgaaggc ggcattctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005155
VERSION     KHUT01005155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           95 g           38 t
ORIGIN      
        1 gacagggggc tcaagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggcccgt
       61 caagcattct gtgaaagccc ccggctcaac cggggaacag cagagtggac tgacgggctc
      121 gaggcaagta ggggcgggca gaacagtcgg tggagcggtg aaatgcgtag atatcgactg
      181 gaatgccgat ggtgaagacg gcccgctggg cttgtcctga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005156             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005156
VERSION     KHUT01005156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           98 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggtcgac
       61 gaagtctatg ggtgaaaaac cggagctcaa ctccgtgaga gcccaggata ctggtcgact
      121 ggagacacgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005157
VERSION     KHUT01005157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcag
       61 taagtcttta gtgaaatctc cgggctcaac tcggagcctg ctaaggaaac tgctgtgctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactaga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005158
VERSION     KHUT01005158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtcagag gtgaaagccc ggagctcaac tccggaattg cctttgagac tgcatcgctt
      121 gatcgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgatga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005159
VERSION     KHUT01005159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          103 g           35 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggaccgc
       61 cacgtgccgt gtgacagacc accgctcaac ggtggagggc catggtacac gagcggtctg
      121 gaggcagaga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc tctgttctga cgctgaggcg cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01005160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005160
VERSION     KHUT01005160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttac
       61 caagtcaggc gtgaaaggcc tgggctcaac ccgggaactg cgcttgatac ttgtagactt
      121 gagtccggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005161
VERSION     KHUT01005161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttag
       61 taagtgtgat gtgaaagccc cgggctcaac ctgggaattg cctcacatac tgctaagcta
      121 gagtacggga gaggggggta gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccggt ggcgaaggcg gccccctgga tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005162
VERSION     KHUT01005162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          102 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggagcgt
       61 taagtccgat ctgaaagccc ccggctcaac cggggagagt ggttggaaac tggcgctctt
      121 gagggcgata gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccagt ggcgaaggcg gccctctggg tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005163
VERSION     KHUT01005163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           89 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggactct
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaattg cctttgatac tgtgagtctt
      121 gagaccggaa gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005164
VERSION     KHUT01005164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           92 g           54 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccgac
       61 taagtagggt gtgaaatgcc agagctcaac tttggcattg cgctctagac tggttggctt
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005165             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005165
VERSION     KHUT01005165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcac
       61 gaaagtcagg tgtgaaagcc ctgggctcaa cccaggaagt gcacttgaaa ctgcggagct
      121 tgaatgctgg agagggaagc ggaattctcg gtgtagaggt gaaattcgta gatatcggga
      181 ggaacaccgg tggcgaaggc ggcttcctgg acagttattg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005166             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005166
VERSION     KHUT01005166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          105 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcccgt
       61 taagtctgac ggtgaaatgc cggggctcaa ccccgagcgt gccggagata ctggcgggct
      121 ggagagacgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgttgtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005167             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005167
VERSION     KHUT01005167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           85 g           54 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcttgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctggcaagct
      121 tgaggaatgc agaggtttgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcaaactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005168
VERSION     KHUT01005168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          100 g           42 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgtc
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaactg cactcgatac tggtcggcta
      121 gagtccggga ggggatggtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggaactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01005169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005169
VERSION     KHUT01005169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           88 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 tgagtctgat gtgaaatctc cgagcttaac tcggaaactg catcggatac tatccggctt
      121 gaggaatgga ggggagattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gatctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005170
VERSION     KHUT01005170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctcgg
       61 ttagtccgtc gtgaaagtca aaggctcaac ctttgaatgc cgatggatac tgccgagcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005171             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005171
VERSION     KHUT01005171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           53 t
ORIGIN      
        1 gacagaggtg gctagcgttg ttcggattca ttgggtgtaa agggtgcgta ggcggtttag
       61 aaagtcagat gtgaaatccc acagctcaac tgtggaactg catctgaaac ttctgaactc
      121 gagtgcgaga gaggtaaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt agcgaaggcg ctttactggc tcgtaactga cgctgagtgc acgaaggtat
      241 ggggagcaaa cggg
//
LOCUS       KHUT01005172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005172
VERSION     KHUT01005172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          103 g           37 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 cgcgtccggg gtgaaatgcc acggctcaac cgtggaacgg cctcggatac gggcggtctc
      121 gaggggggta ggggcatctg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg aggtgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005173
VERSION     KHUT01005173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagtcgaac gtgaaatccc cgggctcaac ccgggaactg cgtccgaaac tggcaggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcggt ggcgaaggcg gcatcctgga ccgatattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005174
VERSION     KHUT01005174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtccg
       61 taagtcggaa gtgaaatttc ggggctcaac cccgaagctg cttctgatac tgtggatcta
      121 gggaccggta gaggctggtg gaattacagg tgtagcggtg gaatgcgtag atatctgtaa
      181 gaacacccgt ggcgaaggcg gccagctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005175
VERSION     KHUT01005175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           77 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgaaaagctt
      121 gaatagacct gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaaa gtctaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005176
VERSION     KHUT01005176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggacgac
       61 caagttgggg gtgaaagccc ggggctcaac ctcggaattg ccttcaaaac tggtcgtctt
      121 gagtatgaga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005177
VERSION     KHUT01005177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           97 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtgcca
       61 taagtctgtt gtgaaatctc ccggctcaac tgggagggtg cggtggaaac tgtggtgctg
      121 gagtgtggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtagacg gcttactgga ccactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005178             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005178
VERSION     KHUT01005178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatgag
       61 aacgtctgtc gctgaaatcc ctcggctcaa ccggggaact ggcggagata cggctcgtct
      121 tgaggggcat agggggatct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcctg tggcgaaagc gaggtcctgg atgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005179
VERSION     KHUT01005179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           56 c          109 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcgcga
       61 caagtcggcc gtgaaagcgc caggcttaac ctggcgaggt cgggcgagac tgtcgggctt
      121 gaggcaggca gagggcggtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctggg cctgtcctga cgctgaggtg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01005180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005180
VERSION     KHUT01005180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct
       61 taggtctgat gtgaaagcct tcggcttaac cggagaagtg catcggaaac caggagactt
      121 gagtgcagaa gaggacagtg gaactccatg tgtagcggtg aaatgcgtag atatatggaa
      181 gaacaccagt ggcgaaggcg gctgtctggt ctgtaactga cgctgaggct cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005181
VERSION     KHUT01005181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           95 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttctc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tggaggactg
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgtaactga cgctgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005182
VERSION     KHUT01005182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          101 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcggat gtgaaaacct gcggctcaac cgcaggctgt cattcgatac tgccgggctc
      121 gaggcaggta ggggagattg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005183
VERSION     KHUT01005183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggt gcaaacgttg ttcggaatca ctgggcataa agagcgcgta ggcggtctga
       61 taagtcctgt gtgaaagccc ccggctcaac cggggaattg cacaggatac tgtcgggcta
      121 gagtacagga ggggagagcg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccagt ggcgaaagcg gctctctggt ctgttactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005184             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005184
VERSION     KHUT01005184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           98 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggtctgg
       61 aaagtctatg ggtgaaatgc cggggctcaa ccccgcgcat gcccaggata ctgtcagact
      121 ggagacacgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005185
VERSION     KHUT01005185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggccgtc
       61 cacgtcgggt gtgaaagctc agggctcaac cctgagaggc cactcgatac gggacggcta
      121 gaggcagcta ggggagagtg gaatacctgg tgtagcggtg aaatgcgcag atatcaggtg
      181 gaacaccggt ggcgaaggcg gctctctggg gctgatctga cgctgaggcg cgaaagctgg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005186
VERSION     KHUT01005186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           97 g           43 t
ORIGIN      
        1 tacgtaggat gctagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcatac
       61 caagtacgat gtgaaatctc cctgctcaac ggggagggtg catagtaaac tggtaagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005187
VERSION     KHUT01005187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          103 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtcccc
       61 taagtgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcaaac tgggggactc
      121 gagcatgtca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg acatggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005188
VERSION     KHUT01005188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgtgta ggtggtacga
       61 atagtctgct gtgaaagcgc ccggctcaac tgggcgaggt cagtggaaac tgtcgcacta
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg ccgctcctga cactgagacg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005189
VERSION     KHUT01005189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctggta ggcggccaac
       61 taagtcggac gtgaaatccc caggctcaac ctgggaactg cgtccgatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgagattga cgctgaacag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005190
VERSION     KHUT01005190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           56 c          106 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgca ggcggccacg
       61 cacgtccgtt gtgaaatcgc ccggctcaac tgggcggggt cggcggatac ggcgtggctg
      121 gagcaagcta gggggtagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggg gctttgctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01005191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005191
VERSION     KHUT01005191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttac
       61 caagtccggg gtgaaatttt gcggcttaac cgtaacagtg ccttggatac tggtaggctc
      121 gagggagata ggggtttgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcaaactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005192
VERSION     KHUT01005192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcctgt
       61 taagcagggt gtgaaatgcc agggctcaac cttggcatgg cgccctgaac tggcgggctt
      121 gagtgagcta ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccggt ggcgaaagcg acacactggg gcttaactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005193
VERSION     KHUT01005193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggcttcg
       61 caagtccgat gtgaaagccc cgggctcaac ccgggatccg cgtcggaaac tgcgaggctt
      121 gagtgcagga gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ctgctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005194
VERSION     KHUT01005194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtac agggcgcgca ggcggttcgt
       61 taagttcgag gtgaaatctc ccggctcaac tgggaggggt cttcgaaaac tggcggactt
      121 gaggagagca gaggtgagtg gaattctggg tgtagtggtg aaatgcgtag atatccagag
      181 gaacaccagt ggcgaaggcg actcactggg ctccacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005195
VERSION     KHUT01005195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaagctc ccggcccaac cgggaaattg ccattgatac tggtagcctt
      121 gagtgcggtt gaggtgggcg gaatacagca tgtagcggtg aaatgcttag atatgctgta
      181 gaacaccgat tgcgaaggca gctcactaaa ccgatactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01005196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005196
VERSION     KHUT01005196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           98 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggagcgta ggcggtgcga
       61 caagtccggt gtgaaatctc cgagcttaac tcggagcggt cattggaaac tgtcgtgctc
      121 gagtgtggga gaggagagtg gaatccccgg tgtagcggtg aaatgcgtag atatcggggg
      181 gaacaccagc ggcgaaggcg gctctctgga ccacaactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005197
VERSION     KHUT01005197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctagg
       61 tcagtcagat gtgaaagccc cgggctcaac ctgggaattg catttgatac tacctggcta
      121 gagtatggta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg acttcctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005198
VERSION     KHUT01005198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           93 g           41 t
ORIGIN      
        1 gacggagggc tccaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcccgc
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaacag catccgatac tggcgggctc
      121 gaggacggca ggggtaagtg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactggg ccgtctctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005199
VERSION     KHUT01005199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggactgg
       61 taagtctggg gtgaaatctc acggctcaac tgtgagcggt ccccggatac tgccggtctt
      121 gaggggctca gaggagagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg actctctggg ggcaacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01005200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005200
VERSION     KHUT01005200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           92 g           46 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaatgg cttggcatac tggacgtctt
      121 gggccaccta ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgcctat ggtgaaaaca gctcgctggg ggtgagccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005201
VERSION     KHUT01005201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggcacgca ggcggtcttg
       61 tgggtttcga gtgacaggtg gtggcttaac tgccacaggc ctcggaaaac ggcaagactt
      121 gagggcttca gagggatgcg gaattccagg tggagcggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gcatcctggg aagcatctga cgctcatgtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005202
VERSION     KHUT01005202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 aacgtagggg acgagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggccttg
       61 caagttggct gtgaaagctc ccggcttaac tgggagaggt cagtcaaaac tgtgaggctg
      121 gaggctgaaa taggagagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tcagccctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005203
VERSION     KHUT01005203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacgtagggg ccaagcgtta tccggattta ctgggcgtaa agagcatgta ggtggtctgt
       61 taagttggac gtgaaagctc ctggctcaac tgggagaggt cgttcaaaac taacagacta
      121 gaggatggta gaggagagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg gctctctggc ccattcctga cactgagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01005204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005204
VERSION     KHUT01005204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           83 g           58 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggttaga
       61 taagttagat gtgaaatacc tgggcttaac ctggggactg cgtttaagac tgtttagcta
      121 gagttctgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagaaactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005205             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005205
VERSION     KHUT01005205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           49 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctcgg
       61 taagccggat gtgaaacctc caagctcaac ttggagcggc cattcggaac tgctgtggct
      121 agagtctggt aggggaacac ggaatacctg gtgtagcggt gaaatgctta gatatcaggt
      181 ggaacaccag tggcgaaggc ggtgttctgg gccagtactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005206             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005206
VERSION     KHUT01005206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           96 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtctgta ggcggtgctc
       61 taagtttggt gtgaaatctc ccggcttaac cgggagggtg cgccgaagac tggggtgctc
      121 ggagtacggg agaggaaagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggtgtagac ggctttctgg accgtgactg acgctgagag acgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005207
VERSION     KHUT01005207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           91 g           53 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ctgagaatca ctgggtttaa agggtgcgta ggtggtccgt
       61 taagtctgtt gtgaaagccc ttggctcaac caaggaattg cttcggatac tggcggactt
      121 gaggctagta gaggtcactg gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaacgccggt ggtgaagacg ggtgactggg ctagttctga cactgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005208             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005208
VERSION     KHUT01005208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          104 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctgg
       61 taagtcggct gtgaaagccc ggagctcaac tccggagggt cggccgatac tatcagtctt
      121 gagggcggca gggagaagtg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg ccgtgtcctg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005209
VERSION     KHUT01005209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctaga
       61 tcagtctgct gtgaaagtca aaggctcaac ctttgaatgc cggtggatac tgtctagcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005210
VERSION     KHUT01005210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           99 g           45 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatca ttgggcgtaa agggtgcgta ggtggtgccg
       61 taagtctgac gtgaaatctc tgggcttaac ccagaatccg cgccagggac tgcggtgctg
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gctctctgga ccacaactga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005211
VERSION     KHUT01005211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           81 g           57 t
ORIGIN      
        1 tacggagggt ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcccgt
       61 taagtcagcg gtgaaatctc ccggctcaac cgggaaatgg ccattgatac tgacgggctt
      121 gaattaccag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact ggtggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005212
VERSION     KHUT01005212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          100 g           46 t
ORIGIN      
        1 tacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcttgt
       61 caagcccgtg gtgaaatgtc ccggcctaac cggggacgtg ctatggggac tgacgagctt
      121 gggcgatcta ggggtctgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acagactggg gattggccga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005213
VERSION     KHUT01005213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagcgta ggttgctcgg
       61 caagtcagtg gtgaaatccc agagctcaac tttggaactg ccattgaaac tgccaagcta
      121 gaatctcaga ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga tgagtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005214
VERSION     KHUT01005214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           96 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggtttgg
       61 agcgttcgcg ctgaaatccc acagcttaac tgcggggcgc gtgcgaatac gtccagactc
      121 gaggcaggga gaggatgggg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg cccatctggt cctgtcctga cgctgtggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005215
VERSION     KHUT01005215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          104 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgcgtcgg ccatgcgaac tgggagactg
      121 gagccgatca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg atcgagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005216
VERSION     KHUT01005216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          110 g           34 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcggg
       61 cgcgcgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccatgcggac ggcccgactg
      121 gagcacggta gaggccgatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggg ggcgaaggcg gtcggctggg ccgtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005217
VERSION     KHUT01005217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           91 g           53 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctctg
       61 taagtcagga gtgaaagccc cgagcttaac ttgggaagtg cttttgatac tgcagagctt
      121 gagtgtggga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ccaacactga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005218
VERSION     KHUT01005218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           52 c          102 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggtgaga
       61 tgcgtccgtt gtgaaatcgc ccggctcaac tgggcggggt cagcggatac ggtctcactt
      121 gagcaagcta gggggcaatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctggg gctttgctga cgctgatgcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01005219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005219
VERSION     KHUT01005219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcagct gtgaaaacca gcagcttaac tgttggcttg cagctgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005220
VERSION     KHUT01005220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           86 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagttgaat gtgaaatccc cgggctcaac ctgggaactg catccaaaac tggcaagcta
      121 gagtatggta gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacatcagt ggcgaaggcg accacctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005221             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005221
VERSION     KHUT01005221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           49 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaaccc caggctcaac ctggggacgc cactcgatac tgctgtgact
      121 tgagttcggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005222
VERSION     KHUT01005222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcgg
       61 taagtccgct ctgaaagtcc agggctcaac cctgggatgc gggtggatac tgccgggctc
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005223             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005223
VERSION     KHUT01005223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           48 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggattta ttgggcgtaa agggctcgta ggcggttcga
       61 caagtcgggt gtgaaacctc caggctcaac ctggagtcgc cactcgaaac tgttgtgact
      121 agagtccggt aggggagcat ggaattcctg gtgtagcggt ggaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc gatgctctgg gccggtactg acgctgagga gcgaaagcat
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005224
VERSION     KHUT01005224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           97 g           45 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacca
       61 taagtacgca gtgaaatctc cctgctcaac ggggagggtg ctgtgtagac tatggagcta
      121 gaggtgggta gagggtggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccacctggg cccaatctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005225
VERSION     KHUT01005225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           83 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcgggccct
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tgagggtctt
      121 gagtgccgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacacctat ggcgaaagca ctcttctgga cggaaactga cgctgagata cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01005226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005226
VERSION     KHUT01005226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgg
       61 taggtctcgt gttaaatctt gcggctcaac cgcaaactgt cacgggaaac cccctcgcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg atatgcgcag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005227
VERSION     KHUT01005227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tggcgatctt
      121 gaggtgctca ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgcctgc ggtgaaaacg gctcgctggg agcaatctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005228
VERSION     KHUT01005228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtcgggg gtgaaatctt tcagctcaac tggaaaagag cttccgatac tggtggtctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005229
VERSION     KHUT01005229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           93 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggtttga
       61 aaagtccggg gtgaaatctt gcggctcaac tgcaagaggt ccttggatac tttcagactt
      121 gagggaggca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctttcctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01005230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005230
VERSION     KHUT01005230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           57 c           86 g           46 t
ORIGIN      
        1 gacggaccac acaaacgtta ttcggaatca ctgggcttaa agggtgcgca ggctgtgcag
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac tactgtgctc
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctcaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005231
VERSION     KHUT01005231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgta ggcggacctg
       61 caagtcagat gtgaaatctc ggggctcaac ctcgaaactg cgtctgaaac tacaggtcta
      121 gaatctcgga gggggaaggg gaatttcgca tgtaggggta aaatccgtag atatgcgaag
      181 gaacaccaga ggcgaaggcg ccttcctgga cgagtattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005232             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005232
VERSION     KHUT01005232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           84 g           62 t
ORIGIN      
        1 tacagagggt gcaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggtggtttt
       61 gtaagttaaa cttcaaagac ccaggcttaa cctggggaag gggtttaata ctgcaggact
      121 tagagaattt taggggcaat cggaactgtt ggtgtagggg tgaaatccgt tgatatcaac
      181 aggaacgtca agggcgtagg cagattgctg ggaaatttct gacactgagg aacgaaagct
      241 aggggagcga aaggg
//
LOCUS       KHUT01005233             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005233
VERSION     KHUT01005233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           81 g           58 t
ORIGIN      
        1 gacataggtc gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtttga
       61 taagttagca gtggtaaatc gaggctcaac ctcgtggaag ttgataatac tgtcatactg
      121 gagcacagga gaggttaacg gaatttccca tggagcggtg aaatgcgtag atatgggaag
      181 gaacatcaat aacggcgaag gcagttaact attctgttgc tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01005234             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005234
VERSION     KHUT01005234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           88 a           29 c           70 g           69 t
ORIGIN      
        1 tacgagaggg gcaagcgtta ttcggaatga ttgggcgtaa agagcacgta gacggttttt
       61 ttattttata tttgaaaact caaagcttaa ctttgagatg aaatatctta aaatataaaa
      121 cttgagtaag tgagaggaga atggaatttc tggaggagag gtaaaatttg ttgatttcag
      181 gaggaacacc aaaagtgaaa acagttctct ggcacttaac tgacgttgag gtgcgaaagc
      241 gtggggatca aaaggg
//
LOCUS       KHUT01005235             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005235
VERSION     KHUT01005235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           86 a           31 c           71 g           68 t
ORIGIN      
        1 tacgagaggg gcaagcgtta ttcggaatga ttgggcgtaa agagcacgta gacggttttt
       61 tattgttcac ttgaaaactc aaagcttaac tttgagaaag agttgtttct taataaaaaa
      121 ctagagtgag tgcgaagaaa atggaattcc tagaggagag gtaaaatttg ttgattttag
      181 gaggaacacc aaaagcgaag gcagttttct aatactttac tgacgttgag gtgcgaaagc
      241 gtggggatca aaaggg
//
LOCUS       KHUT01005236             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005236
VERSION     KHUT01005236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           41 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggcttgg
       61 caagtggctc gtgaaagcct tcggctcaac cggggtggac gggtcagact gcggagctgg
      121 aggatgtcag aaggacacgg aattccgggt gtagtggtga aatgcgtaga gatccggagg
      181 aacaccagtg gcgcaggcgg tgtcctgggg cgtacctgac gctgagacgc gaaagcatgg
      241 ggagcgaacg gg
//
LOCUS       KHUT01005237             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005237
VERSION     KHUT01005237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           99 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggtggcttgg
       61 caagtcggac gtaaaagtcc agggctcaac cctgggatgc gttcgatact gccaggctag
      121 aggccggtag ggggatctgg aattcctggt gtagcggtga aatgcgcaga tatcaggagg
      181 aacaccggtg gcgaaggcgg gatcctgggc cggacctgac gctgatgagc gaaagctggg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005238             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005238
VERSION     KHUT01005238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           48 t
ORIGIN      
        1 tacggggggg caagcgttgt tcggaattac tgggcgtaaa gggctcgtag gtggccaact
       61 aagtcatacg tgaaatccct cggcttaacc ggggaactgc gtctgatact ggatggcttg
      121 aggttgggag agggatgcgg aattccaggt gtagcggtga aatgcgtaga tatctggagg
      181 aacaccggtg gcgaaggcgg catcctggac caattctgac actgaggagc gaaagccagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01005239             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005239
VERSION     KHUT01005239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcag
       61 taagttggat gtttaatgcc ggggctcaac cccggtacgc atccaaaact ggtggacttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005240             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005240
VERSION     KHUT01005240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcgact
       61 taagtctggt gtttaagccc gaggctcaac ctcggttcgc atcggaaact gggtcacttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005241             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005241
VERSION     KHUT01005241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c          101 g           37 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaatgg
       61 taagcggggc cggaaagccc ccggctcaac cggggaagag gccgtgaact gccattcttg
      121 agggggggag aggagagcgg aattcctggt gtagcggtga aatgcgtaga gatcaggagg
      181 aacacccatg gcgaaggcag ctctctggcc tcaccctgac gctgaggcgc gaaagcgtgg
      241 ggagcgaacg gg
//
LOCUS       KHUT01005242             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005242
VERSION     KHUT01005242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtcggg
       61 taagtctgct gtgaaagtcc agggctcaac cctgggatgc cgcagatact gcttggctag
      121 agtacgggag gggcgagtgg aattcctggt gtagcggtgg aatgcgcaga tatcaggagg
      181 aacacctatg gcgaaggcag ctcgctggaa cgttactgac gctgagacgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005243             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005243
VERSION     KHUT01005243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           43 t
ORIGIN      
        1 gacgaaggtg gctagcgtta ttcggattta ctgggcataa agcgcgcgta ggcggcttgg
       61 caagtcggag gtgaaagcct gcagctcaac tgcggtgtgc ctccgaaact gccgagcttg
      121 agtgcgggag gggtgattgg aactccaggt gtagcggtga aatgcgtaga tatctggagg
      181 aacaccagag gcgaaggcga atcactggac cgcaactgac gctgaggcgc gaaagctagg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005244
VERSION     KHUT01005244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          100 g           43 t
ORIGIN      
        1 gacggggggg gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtgcgg
       61 caagtcgggt gtgaaatctc cgagcttaac tcggagcggt cattcgaaac tgccgtgcta
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctgga ccacaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005245
VERSION     KHUT01005245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggattta ctgggtgtaa agggcacgta ggcggctcaa
       61 taagccctgt ttgaaatgtg cgggcttaac ctgcacacgt ggcaggggac tgttgagctt
      121 gaggatggca gaggtaggcg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctactggg ccatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005246
VERSION     KHUT01005246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 tacgtagagg gcaagcgttg tccggaatta ctgggcgtaa agggtacgca ggcggatata
       61 taagtcaaat gtgaaaggca ccggctcaac cggtgagcgg catttgaaac tgtatatctt
      121 gagttcagga gaggagagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccagt ggcgaaggcg gctctctggc ctgaaactga cgctcaggta cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005247
VERSION     KHUT01005247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           86 g           55 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ctgggcgtaa agagtgcgta gacggtagat
       61 taagtttgtt gttaaagact ctagcctaac tggagcgctg caatgaaaac tggtttgctg
      121 gagggtgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005248
VERSION     KHUT01005248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggcggcttgt
       61 taagtcaggg gtgaaagttt tcggcccaac cggaaaattg cctttgatac tggcgagctt
      121 gagtgcgtga ggggtaaccg gaacgagtcg tgtagcggtg aaatgcgtag atatgactcg
      181 gaaggccaac ggcgaaggca ggttactggt acgcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005249
VERSION     KHUT01005249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           81 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgca ggcgggctgg
       61 taagtcagtg gtgaaagcct tgagcttaac tcgagaactg ccattgatac tatcagtctt
      121 gaatattgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaaa caaagattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005250             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005250
VERSION     KHUT01005250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           42 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcgggt gtgaaaactc ggggctcaac cccgagacgc cactcgatac tgccatggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005251
VERSION     KHUT01005251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtctgg
       61 caagtcggat gtgaaatctc agggctcaac cctgaaactg cattcgatac tgccaggctt
      121 gagtactgga ggggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005252
VERSION     KHUT01005252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           84 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatgag
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacacgg ctccctatac tgcttgtctt
      121 gagtgccgga gaggacaagg gaacttcacg tgtagcggtg aaatgcgttg agatgtgaag
      181 gaacaccaat ggcgaaagca cttgtctgga cggaaactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01005253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005253
VERSION     KHUT01005253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           70 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtctta
       61 taagtcagtg gtgaaatccg gcagctcaac tgtcgaactg ccattgatac tgtaagactt
      121 gaattactgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactaac attatattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005254
VERSION     KHUT01005254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctggg gtgaaatcct ccggctcaac cggagaacgg cctcggatac tggcggtctc
      121 gagggaggta ggggcgtgcg gaactaaggg tggagcggtg aaatgcgttg atatccttag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005255
VERSION     KHUT01005255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggttgttatt
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catacaatac tgggtaacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005256
VERSION     KHUT01005256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtatgg
       61 taagtcggat gtgaaatctt ggggctcaac cccaaaactg cattcgatac tgccgtgctt
      121 gaaggctgaa gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaagcg aatctctgga cagctcttga cgctgatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005257
VERSION     KHUT01005257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           51 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgct
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaactg ccatggatac tgggtgtctc
      121 gaggaagata ggggtatgcg gaacgattgg tggagcggtg aaatgcgttg atatcaatcg
      181 gaactccggt ggcgaaggcg gcatactgga tcttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005258
VERSION     KHUT01005258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 gacgaactgc ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcagat
       61 caagtcaggg gtgaaatccg gcagctcaac tgtcgaagtg cctttgatac tgatttgctc
      121 gagggagata ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005259
VERSION     KHUT01005259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggttccg
       61 ccagtctggt gtgaaagccc agggctcaac cctggaagcg cattggatac tacggagctt
      121 gagtcctgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caggtactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005260
VERSION     KHUT01005260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttatg
       61 caagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cattcgatac tgcgtagcta
      121 gagtactgga gaggatggtg gaactctggg tgtagaggtg aaattcgtag atatccagaa
      181 gaacaccggt ggcgaaggcg gccatctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005261
VERSION     KHUT01005261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           87 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcttag
       61 taagtcagtg gtgaaagact gtggcttaac catggaattg ccattgatac tgctgagctt
      121 gaatcaggtt gaggttggcg gaatgtgaca tgtagcggtg aaatgcttag atatgtcatg
      181 gaacaccgat tgcgaaggca gctgactgga cctgtattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005262
VERSION     KHUT01005262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           96 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctct
       61 ccagtcggga gtgaaatcgc tcggcccaac cgagcaaggg cttccgatac tgagagcctc
      121 gagggaggta ggggtcgagg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005263
VERSION     KHUT01005263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggtggcttcg
       61 caagtcgggt gtgaaatccc tgggctcaac ccaggacgtg catccgaaac tgcgtcgctt
      121 gagtactgga gagggcagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctgcctgga cagttactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005264
VERSION     KHUT01005264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacagagacg tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagtcggat gtgaaatccc aaagcttaac tttggaagtg catccgaaac tccctcgctg
      121 gaggactgga agggagactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005265
VERSION     KHUT01005265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttgc
       61 caagtcaggg gtgaaatctt tcggcccaac cggaaaaggg cccctgatac tggcagtctc
      121 gagggaggta ggggtcgagg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005266
VERSION     KHUT01005266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           98 g           44 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaacgg ccgtggatac tggcggtctc
      121 gagggagata ggggcgtgcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005267
VERSION     KHUT01005267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           97 g           41 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggccagg
       61 taagtccggt gtgaaagtca gaggctcaac ctctgaatgc cgctggaaac tgcctggcta
      121 gagtccggga ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctgga acggaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005268
VERSION     KHUT01005268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggccaac
       61 taagtctgat gtgaaatccc agggctcaac cctggaactg catcggatac tggatggcta
      121 gaggatgaga gagggtggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccacctggc tcatttctga cgctgagacg caaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005269
VERSION     KHUT01005269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggcctgt
       61 caagtcaggg gtgaaatccg gcagctcaac tgtcgaagtg cctttgatac tgacgggctc
      121 gagggaggta ggggtcgctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agcgactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005270
VERSION     KHUT01005270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           88 g           46 t
ORIGIN      
        1 tacagggggt gcaagcgttg ctcggaatta ctgggcgtaa agcgcacgca ggcggatgaa
       61 caagtcggac gtgaaatccc tgggctcaac tcaggaactg cattcgaaac tgttcatctg
      121 gagtacggga gaggtgagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaagcg gctcactggc ccgatactga cgctcatgtg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005271
VERSION     KHUT01005271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           90 g           49 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggaatta ctgggcgtaa agggagtgca ggcggttgaa
       61 taagttaggc gtgaaagact tcggctaaac cgggggaggt cgtctgatac tgttcaacta
      121 gagtatggta gaggcgtgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgaaggcg gcacgctggg ccataactga cgctaagact cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005272
VERSION     KHUT01005272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           93 g           52 t
ORIGIN      
        1 tacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgtgtgca ggcggtttgg
       61 ttagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaatac tgccagacta
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccgttcctga cgctaagaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005273
VERSION     KHUT01005273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcac
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg ccttggatac tggtggtctt
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctctactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005274
VERSION     KHUT01005274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           91 g           43 t
ORIGIN      
        1 tacgaaccgg acaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggtctgg
       61 agggtggggt gtgaaatccc tcggctcaac cgaggaattg cgctccaaac cggcagactt
      121 gagggagaca gaggtgtgcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcacactggg tctctactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005275
VERSION     KHUT01005275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           39 c           81 g           65 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggttttt
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgaaagactt
      121 gagtaaagtt gaggtgggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat tgcgaaggca gctcactaag ctttaactga cactgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005276
VERSION     KHUT01005276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggttat
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tggtaatcta
      121 gagatcggaa gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005277
VERSION     KHUT01005277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatg
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catctcatac tgcatagctg
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005278
VERSION     KHUT01005278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatttg
       61 caagtccggg gtgaaatccc acagctcaac tgtggaactg cctcggatac tgtgagtctc
      121 gagggtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccacttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005279
VERSION     KHUT01005279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcgggtcgt
       61 taagtccgac gtgaaatccc tcggctcaac tgaggaagtg catcggatac tggcggtctt
      121 gagtcctgga gagggtagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagtgactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005280
VERSION     KHUT01005280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           83 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcataa
       61 aaagtcagat gtgaaatccg gaggctcaac ctccgaattg catccgaaac tcctatgctt
      121 gagggttgta gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgc agcgaaagcg gggctctggg caacttctga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005281
VERSION     KHUT01005281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcttgg
       61 aaagtcggat gtgaaatccc ggggctcaac cccggaagtg cactcgatac tgccaagcta
      121 gagtcctgga gaggaaggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagta
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005282
VERSION     KHUT01005282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctga
       61 aaagtcaggg gtgaaatccc tcggctcaac cgaggaactg cctctgaaac tgccaggctc
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctgt ggcgaaggcg actacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005283
VERSION     KHUT01005283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           94 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ttgggcgtaa agcgcacgca ggcggctttt
       61 taagtttgac gtgaaaactc ctagcttaac tgggagagga cgttgaagac tggaaggctt
      121 gaggtaatga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc attgacctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005284
VERSION     KHUT01005284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctcat
       61 taagtcagag gtgaaatccc ggggctcaac cccggaactg cctttgaaac tggcgagcta
      121 gaatcctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005285
VERSION     KHUT01005285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcggagctg
       61 taagtcagag gtgaaatcct gcggctcaac cgtagaactg cctttgatac tgcagccctt
      121 gagtctcgga gaggttgctg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga ggcgtaggcg ggcaactgga cgagaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005286
VERSION     KHUT01005286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacggagacc tcgagcgtta ctcggattta ctgggcgtaa agggttcgta ggtggccaag
       61 tgtgtcgggt gtgaaatctt gcggcttaac cgtaaaactg cgcccgaaac tatttggcta
      121 gagactcgga ggggtaggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaac ggcgaaggca gcctactgga cgagatctga cactgaggaa cgaaagtatg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01005287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005287
VERSION     KHUT01005287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcgg
       61 aaagtcgggt gtgaaagccc tgggctcaac ctaggaagtg cactcgaaac ttccgagctg
      121 gagtttcgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005288
VERSION     KHUT01005288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtgctg
       61 taagtcaggt gtgaaatctc ggggctcaac cccgaaactg cacttgatac tgcagtgctc
      121 gaggactgga gaggagatcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005289
VERSION     KHUT01005289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacgtaggtg tcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcatgg
       61 aaagtcgtac gtgaaagctc ccggcttaac tgggagagga cgtaggaaac tatcaggctg
      121 gagacagtga gaggaaggcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gccttctggc actgttctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005290
VERSION     KHUT01005290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           82 g           57 t
ORIGIN      
        1 tacgtggagt ccaagcgtta tccggattta ctgggcgtaa agcgtctgta ggtgtcccgt
       61 taagtcttcg gtgaaatctc caggctcaac ttggaaactg tcgaggaaac tagcgggatc
      121 gagtacggta gaggtagatg gaattcttgg tgtagaggta aaattcgttg atatcaagaa
      181 gaacaccagt ggcgaaggcg atctactggg ccgttactga cactgagaga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005291
VERSION     KHUT01005291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           81 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtccgta ggcggatttt
       61 taagtcagtg gtgaaagccc gcagctcaac tgtggaattg cctttgatac tgggaatctt
      121 gagtgtgcgt gaagtaggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactaaa gcactactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005292
VERSION     KHUT01005292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           84 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggagtgt
       61 taagtaagaa gtgaaaggtt ggagctcaac tctgacacgg cttcttatac tggcattctt
      121 gagtcttgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccgat ggcgaaagca gctttctgga caagtactga cgctgaggtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01005293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005293
VERSION     KHUT01005293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           82 g           64 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcgat
       61 taagtcagtg gtgaaatact ctagcttaac tagaggggtg ccattgatac tgattgactt
      121 gagtgaagtc gaggttggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gctaactagg cttatactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005294
VERSION     KHUT01005294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c          102 g           38 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggcgctt
       61 caagttcgtc gtgaaagacc ccggctaaac cgggggtggt cgagggagac tgaagcgctg
      121 gaggacggta gagggacagg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg ctgtcctggg ccgcacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005295
VERSION     KHUT01005295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggacggg
       61 taagtatctt gtgaaatccc acggctcaac cgtggaactg ctggatatac tgcttgtctt
      121 gaggcattca ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgcctat ggtgaaaaca ggttgctggg gatgttctga cactgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005296
VERSION     KHUT01005296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ctgggcgtaa agcgcacgta ggcggagcgg
       61 aaagttgggg gtgaaagccc agggctcaac cctggaactg ccttcaaaac tcccgctctt
      121 gaggtcgtga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc acgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005297
VERSION     KHUT01005297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggtggttttg
       61 taagtctggc gtgaaatttt tcggcttaac cgggaaaggt cgtcggatac tgcaaggcta
      121 gaggatggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccatacctga cactgatgcg cgacagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005298
VERSION     KHUT01005298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcagg
       61 ttagtgagat gtgaaacgcc ccggcttacc cggggaactg catctcatac tgcctggctt
      121 gagtacggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg ctcatctggc ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005299
VERSION     KHUT01005299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctgga
       61 taagtctgac gtgaaatccc tcggcctaac cgaggaactg cgtttgaaac tgtccggctc
      121 gagtccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgatgcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01005300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005300
VERSION     KHUT01005300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           85 g           56 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggatcta
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg cacttgatac tgtagatctt
      121 gagtgtggta gaggttactg gaattcttgg tgtagtggtg aaatacgtag atatcaagag
      181 gaataccgga ggcgaaggcg ggtaactggg ccaacactga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005301
VERSION     KHUT01005301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccttt
       61 taagtcagat gtgaaatccc ttggctcaac cgaggaactg catctgatac tgggaggctt
      121 gagtatcgga ggggatggta gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaatacctgt ggcgaaggcg accatctgga agataactga cgctgagatg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005302
VERSION     KHUT01005302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           90 g           44 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcagat gtgaaatccg ggggctcaac ccccgaatgg catccgatac ttccgtgctc
      121 gagggctgaa ggggggtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacactagt ggcgaaagcg agacccttgg cagcatctga cgctgaagca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005303
VERSION     KHUT01005303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           92 g           44 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcgcgta ggcggacacg
       61 taggtcagtt gtgaaatacc tgggctcaac ccgggaatgg cagctgaaac cgcgtgtctt
      121 gaatacctga gagggtgggg gaatacctgg tggagaagtg aaattcgtag agatcaggtg
      181 gaacaccgga ggcgaaggcg cctgcctggc agtgtattga cgctgaagtg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01005304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005304
VERSION     KHUT01005304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           86 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggggcgca ggcgggcctg
       61 taagtcagtg gtgaaatccc cgggctcaac cctggaattg ccattgatac tatgggcctt
      121 gaatgccgtt gaggtatgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca gcatgctaag cggatattga cgctgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005305
VERSION     KHUT01005305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatta ctgggcttaa agggtgcgta ggctggcccg
       61 caagtgcttt gtgaaatccc ccggctcaac cggggaacag cttggcatac tgcgggtctt
      121 gaggaaggca ggggtcgcca gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaatgccgat ggtgaagacg ggcgactggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005306
VERSION     KHUT01005306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           92 g           53 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggagtta ctgggcttaa agggtgcgta ggcggcgctg
       61 taagtgcttt gtgaaatccc atggcttaac catggaattg ctcggcagac tgcagcgctt
      121 gaggtcggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgtagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005307
VERSION     KHUT01005307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggccttg
       61 taagttggat gtgaaagccc tgggctcaac ccaggaagtg catccaaaac tgcatggctc
      121 gagtgccgga ggggttcaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgaactgga cggtaactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005308
VERSION     KHUT01005308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           92 g           51 t
ORIGIN      
        1 gacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgcgta ggtgggtaga
       61 taagtggttg gtgaaagacc tgggcttaac cgagggattg ccaatcatac tgtttatcta
      121 gagtagggta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctatctgga ctcatactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005309
VERSION     KHUT01005309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggctttt
       61 taagttggac gtgaaagctc ccggcttaac tgggagaggc cgttcaaaac tgagaggcta
      121 gagtgaggaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt cctaaactga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005310
VERSION     KHUT01005310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           81 g           51 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggcggtcatg
       61 aaagttagaa gtgaaatccc cgggcttaac ccgggaatgg cttttaaaac tccatgacta
      121 gagtactgga gaggttggcg gaattccaag tgtagcagtg aaatgcgtag atatttggag
      181 gaacaccaat ggcgtaggca gccaactgga cagttactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005311
VERSION     KHUT01005311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           83 g           54 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggaatta ttgggcgtaa agcgcatgta ggcggtttac
       61 taagtctggt gtgaaagctc acggcctaac cgtgaaattg cattggaaac tggtgaacta
      121 gaatactgga ggggtaagtg gaattccatg tgtagcggtg gaatgcgtag atatatggag
      181 gaacaccaat ggcgaaggca gcttactgga cagagattga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005312
VERSION     KHUT01005312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           94 g           55 t
ORIGIN      
        1 tacgtgaggg gcgaacgttg ttcggtgtca ctgggcttaa agggtgcgta ggtggttgcg
       61 taagtaaggt gtgaaatccc tcggcttacc cggggaattg cattttatac tgcgtggctt
      121 gaggactaaa ggggaaaagg gaactccagg tgtagcggtg aaatgcgcag atatctgggg
      181 gaaggccggc ggcgaaggcg cttttctggt tagtacctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005313
VERSION     KHUT01005313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcca
       61 taagtcgggc gtgaaatccc caggctcaac ctgggaactg cgctcgaaac tgtgggtctt
      121 gagtgtcgga gaggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccatctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005314
VERSION     KHUT01005314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctta
       61 taagtcagtt gtgaaatacg gcagcttaac tgtcgaggtg caattgatac tgtaagactt
      121 gagtactgtt gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag agatcgtcca
      181 gaacaccgat agcgaaggca gcttactaaa gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005315
VERSION     KHUT01005315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           93 g           54 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttcgt
       61 taagtctgat gttaaagact ggggctcaac cccgggagtg cattggatac tggcggactg
      121 gagtgtggta gaggctagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgtaggcg actagctggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01005316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005316
VERSION     KHUT01005316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           50 c          103 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtcga
       61 aaagtccgct gtgaaagccc gaggcttaac ctcggcgggt cggtggatac tgtcggtctt
      121 gaaggtgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005317
VERSION     KHUT01005317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          102 g           39 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggttgcg
       61 tgcgtcggcg ctgaaatccc tgggcttaac ccaggggcgc gtgccgatac gatgcgactg
      121 gaggctcgga gagggtggga gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaatacctgt ggcgcaagcg cccacctggc cgagacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005318
VERSION     KHUT01005318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacggagggt gcgaacgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttcgt
       61 taagtgaggt gtgaaatccc cgggctcaac ctgggaatgg catttcagac tggcggactc
      121 gagtacggta gaggggggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg gccccctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005319
VERSION     KHUT01005319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           97 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttc
       61 taagtctgat gtgaaagccc ggggcttacc cccggaagtg cattggaaac tgggaggctt
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005320
VERSION     KHUT01005320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           91 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcatgg
       61 taagttggat gtgaaaaccc tgggctcaac cctggaagtg catccaagac tgccacgctg
      121 gagtgtcgga gggggtcgga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacatctgt ggcgaaggcg ccgacctgga cgacaactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005321
VERSION     KHUT01005321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c           99 g           38 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcgaca gtgaaaactc ggggctcaac cccgagcctg ctgccgatac tgtcgggctc
      121 gaggcaggta ggggagattg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005322
VERSION     KHUT01005322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           85 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagacaaat gtgaaatccc cgggctcaac ctgggacctg catatgtgac tgcaaagcta
      121 gagtacggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005323
VERSION     KHUT01005323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           93 g           49 t
ORIGIN      
        1 tacagggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggccttt
       61 taagtccttt gtgaaatccc ggagctcaac tccggatgtg cataggatac tggagggctt
      121 gagactggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccagatctga cactcagccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005324
VERSION     KHUT01005324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtttgc
       61 taagtcttct gtgaaatcta cgggcttagc ctgtaacgtg cagaggaaac tgacagactt
      121 gagtgtcgga gaggataggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005325
VERSION     KHUT01005325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcaagta ggcggcttgg
       61 taagtccgtt gttaaagccc ccggctcaac tgggggaggt caatggagac tactagactt
      121 gagggcaaca gaggcacatg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gtgtgctggg ttgcaactga cgctgatttg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005326
VERSION     KHUT01005326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           92 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggatcag
       61 caagttggat gtgaaagctc ccggctcaac tgggagaggt cgttcaagac tgctggtctt
      121 gagtgtggta gagggaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccacaactga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005327
VERSION     KHUT01005327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattta ctgggcgtaa agcgcaagca ggcggcttgg
       61 taagttggct gtgaaatctc ccggctcaac tgggagggga cggtcaagac tgtcaggctg
      121 gaggcaggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga cctgacctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005328
VERSION     KHUT01005328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacagaggtg tctagcgttg tccggaatta ctgggcgtaa agggcgcgta ggtgggcggg
       61 taggtcggat gtgaaatcgc tgggcttaac ccagcaactg catccgaaac cactcgtctt
      121 gagtgcagaa gaggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgcaactga cactgaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005329
VERSION     KHUT01005329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           73 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggataat
       61 taagtcagtg gtgaaatctt gcggctcaac cgtaaaattg ccattgaaac tgattatctt
      121 gagtacagtt gaggtgggcg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacacctat tgcgaaagca gctcactaaa ctgcaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005330
VERSION     KHUT01005330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           98 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggagtta ctgggcgtaa agggcgcgta ggcggtgcag
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaattg cgcttgatac tgctgcgcta
      121 gagatcggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgcatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005331
VERSION     KHUT01005331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agggagcgta ggcggccatt
       61 taagtttggt cttaaagacc ggggctcaac cccgggactg gactgaatac tgggtggcta
      121 gaggatgtca gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actttctggg acattcctga cgctgaggct cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01005332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005332
VERSION     KHUT01005332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ctgggcgtaa agcgcgcgca ggcggtcatc
       61 tgcgtccggg gtgaaaaccc agagctcaac tctgggactg ccttggatac ggggtgactt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005333
VERSION     KHUT01005333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggatcgg
       61 taagtcagtg gtgaaatctc atagcttaac tatgaaactg ccattgatac tgtcggtctt
      121 gagtgtattt gaagtagctg gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaacaccaat tgcgaaggca ggttactaag atacaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005334
VERSION     KHUT01005334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggctgcttga
       61 taagtcagat gtgaaagccc tcggctcaac cgaggaagtg cgtctgaaac tgtcgagctt
      121 gagtaccgga gaggatggcg gaattctgca agtagaggtg aaattcgtag atatgcagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005335
VERSION     KHUT01005335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggtaaag
       61 caagtaagat gtgaaagccc tgggcttaac ctgggaagtg catcttaaac tgcttaactt
      121 gagtatggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgccagt ggtgaagacg actacctgga ccaatactga cactgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005336
VERSION     KHUT01005336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           88 g           55 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaata
       61 caagtcagat gtgaaattcc agagctcaac tctggcgctg catctgaaac tgtgtttctt
      121 gagtactgga gaggaaagcg gtattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005337
VERSION     KHUT01005337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           81 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttat
       61 taagtcagtg gtgaaagacg atagcttaac tatcgaggtg ccattgatac tgatgaactt
      121 gagtgcacac gaggtaagcg gaatggacgg tgtagcggtg aaatgcatag atatcgtcca
      181 gaacaccgat agtgaagaca gcttaccaga gtgcaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005338
VERSION     KHUT01005338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggaatta ttgggcgtaa agcgcgtgca ggcggcttgg
       61 caagtctgat gtgaaagccc cgggcttaac ctgggaagtg cattggaaac tgcttggctt
      121 gagtactgga gaggaaggga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cccttctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005339
VERSION     KHUT01005339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccgtg
       61 taagtggggt gtgaaagcct ctggctcaac cagagaaatg cactccatac tgctcggctt
      121 gagtatcgga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc cgaatactga cgctaaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005340
VERSION     KHUT01005340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           92 g           41 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggcttgg
       61 taagttggat gtaaaatctc ccggctcaac tgggagggga cgtacaagac tgccaggctc
      121 gagggcggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gcctcctgga ccgcacctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005341
VERSION     KHUT01005341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c          100 g           39 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgg
       61 gaagttctgg gtgaaagccc ccggctcaac cggggaagtg cctggaaaac cactgggctg
      121 gagtgctgga gaggaaagca gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaatacctgc ggcgaaggcg gctttctgga cagacactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01005342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005342
VERSION     KHUT01005342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggagatc
       61 caagcctggg gtgaaaggtt ggggctcaac cccaagagtg ccctggggac tggatctctg
      121 gaggcaggca gaggagtgtg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacacccat ggcgaaggca gcactctggg cctgtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005343
VERSION     KHUT01005343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           97 g           41 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggttgcg
       61 ggcgtcggtg ctgaaagttc gccgcttaac ggcgaatcgt gtgccgatac gccgcaactc
      121 gagcccgtga gaggtccgtg gaattgccgg tgtagtggtg acatgcgtag atatcggcag
      181 gaacacccaa ggggaagcca gcggactggc acggtgctga cactgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005344
VERSION     KHUT01005344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agggctcgta ggtggctttg
       61 taagtcggag gtgaaatccc acggctcaac cgtggacacg ccttcgaaac tgtgaagctt
      121 gaggctggga gaggaaatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gatttctgga ccagttctga cactgatgag cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01005345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005345
VERSION     KHUT01005345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           40 c           78 g           54 t
ORIGIN      
        1 gacgtaggat gcgagcgtta tccggaatta ttgggcgtaa agagttcgta gacgaactgt
       61 taagtaggat gttaaagacc agggcctaac cctgggaatg tgtcctaaac tggcagaata
      121 gagcaattca gaggcaagta gaattctgtg tgtaggggta atatccgtag atatacagag
      181 gaataccaaa agcgaaggca gcttgctggg aatttgctga cgttcaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005346
VERSION     KHUT01005346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c           99 g           47 t
ORIGIN      
        1 cacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggctggg
       61 tgagttgggc gtgaaagctc ctggcttaac tgggagaggt cgtccgatac tgcctggctt
      121 gaggttggga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005347
VERSION     KHUT01005347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggctcgg
       61 aaagttggct gtgaaagctc ccggctcaac tgggagaggt cggtcaagac ttccgggctc
      121 gagggtggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccatttctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005348
VERSION     KHUT01005348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           91 g           47 t
ORIGIN      
        1 aacgtaggtt ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggctcag
       61 ccagtcgggc atgaaatctc tcggctcaac cgggagaggc tgtccgatac tgctgggctt
      121 gagggcagga gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccacctggt ctgcacctga cgctcaaaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005349
VERSION     KHUT01005349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          101 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctgtg
       61 taagtcgggg gtgaaatgct gcggctcaac cgcagcgctg cctccgagac tgcacggctt
      121 gagcacggta gaggccgatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccgat ggcgaaggca gtcggctggg ccgttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005350
VERSION     KHUT01005350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccgtt
       61 taagtccgat gtgaaagccc tgggctcaac ccaggaagtg cattggaaac tggacggctt
      121 gaatacggga gagggaagtg gaattccgag tgtaggggtg aaatccgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005351
VERSION     KHUT01005351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttagt
       61 cacgtcggat gtgaaaaccc ggagcttaac tccgggcctg cattcgatac gggctgacta
      121 gagtgttgta ggggagactg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg ggtctctggg caactactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005352             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005352
VERSION     KHUT01005352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          102 g           42 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggtctgt
       61 tgcgtcgggc gtgaaagccc cgggctcaac ccgggaatgg cgctcgatac gggcaggctt
      121 tgagtccgga agagggtggt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggccacctgg tccggcactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005353
VERSION     KHUT01005353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           96 g           39 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtcact
       61 taagtctgat gtgaaagcgc atggctcaac caagcgatgg cattggaaac taggtgactg
      121 gagtctggca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ccagtactga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005354
VERSION     KHUT01005354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcgaaatta ctgggcgtaa agggcgcgta ggcggtttag
       61 tgtgtgggat gtgaaagccc tgggcttaac ctaggaagtg catcccaaac tactagactg
      121 gagtagagga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005355
VERSION     KHUT01005355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacggggggt gcaaacgttg ttcggaatca ctgggcgtaa agagcgcgta ggcggtcggg
       61 taagtgggat gtgaaatcca ccggctcaac cggtgaactg catctcatac tgctcggctt
      121 gagggcagca gaggagaggg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctctctggg ctgcacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005356
VERSION     KHUT01005356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacgagtctg acgagcgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 atagtcgggt gtgaaatgcc ttagcttaac taaggaactg cactcgatac tgccgtgcta
      121 gagtaagaca gaggtgagtg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaggcg actcactggg tcttaactga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005357
VERSION     KHUT01005357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtctgg
       61 tcagtctttg ctgacagtgt ccggcttaac cgggcaaggg gtagggaaac tgccagactt
      121 gaggatttca gagggatggg gaactccagg tggagtggtg aaatgcgtag atatctggaa
      181 gaacaccaaa ggcgaaggca ccatcctggg aaatatctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005358
VERSION     KHUT01005358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 gacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcttt
       61 taagtagggt gtgaaagccc ccggctcaac cggggaactg cgccctaaac tagaaggctt
      121 gagtgaggta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactgga ccttgtctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005359
VERSION     KHUT01005359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctttg
       61 taagtcggac gtgaaagccc ggggcccaac cccgggatgc cgttcgatac tgcagagcta
      121 gaggcaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg cctgtcctga cgctgaggag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005360
VERSION     KHUT01005360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttga
       61 caagttggag gtgaaagccc ggagctcaac ttcggaattg ccttcaagac tgtctcgctc
      121 gaggcaggga gaggagagcg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gctctctggc cctgatctga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005361
VERSION     KHUT01005361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggtggcttga
       61 taagtaggag gtgaaatctc acggctcaac cgggaggctg ccttctagac tgtcaggctt
      121 gagcatggta gaggcggatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtccgctggg ccatagctga cactgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005362
VERSION     KHUT01005362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           51 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagttggat gtgaaatctc agggcctaac cctgaaagtg catccaaaac tgccttgctt
      121 gaggactgga ggggagactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ggtctctagc cagttcctga cgctgaggca cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005363
VERSION     KHUT01005363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttcag
       61 aaagcaagat gtgaaatccc tgggcttaac ctaggaatgg cattttgaac ttctgagcta
      121 gagtgtgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ataacactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005364
VERSION     KHUT01005364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c          101 g           40 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttggg
       61 caagtggcga gtgaaagctc ccggctaaac tgggagagga cacgtcagac tgcccggcta
      121 gaggcggata gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggtgaagacg gcgccctggg tccgtactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005365
VERSION     KHUT01005365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggttacg
       61 tatgtctgag gtgaaagccc cgcgcttaac gtgggaagtg ccttggaaac ggcgtaacta
      121 gagtattgga gaggtttgta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaatatcaga ggcgaaggcg acaaactgga caattactga cactaaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005366
VERSION     KHUT01005366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcttt
       61 taagtctgat gtgaaagccc acggcttaac cgtggagggt cattggaaac tggaggactt
      121 gagtgcagaa gaggagagtg gaattccatg tggagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005367
VERSION     KHUT01005367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggttttg
       61 tgtgtctacc gtgaaagcgc gcggcttaac cgcgttccgt cggtagaaac tgcagaactt
      121 gagactggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005368
VERSION     KHUT01005368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcaag
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tgcatgtctc
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga tctcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005369
VERSION     KHUT01005369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggagcgg
       61 cgcatcctgg agtaaatcca acggctcaac cgttggctct tctgggagat ggccgccctt
      121 gaggacagga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005370
VERSION     KHUT01005370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           93 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaactg cctttgatac tgggtgtctc
      121 gagtgaggta ggggcgtgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcgcgctgga ccttaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005371             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005371
VERSION     KHUT01005371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctat
       61 taagttggat gtgaaatctc cacggcttaa ccgggaaact gcatccaaaa ctggcagcct
      121 tgaggtatgg agagggaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacccg tggcgaaggc ggcttcctgg ccatatcctg acgctgaggt gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005372
VERSION     KHUT01005372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgcgg
       61 taagtcctgt gtgaaagccc cgggctcaac ccgggaagtg cgcaggatac tgcagtgctt
      121 gagtgacgga gaggatggcg gaatacccgg tgtagaggtg aaattcgtag atatcgggtg
      181 gaacacctgt ggcgaaggcg gccatctgga cgtcaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005373
VERSION     KHUT01005373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggactga
       61 ttagtacctt gtgaaatccc ccggctcaac cggggaactg ctgggtatac tgttggtctt
      121 gagccaccta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggttgctagg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005374
VERSION     KHUT01005374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 tacggaggtt gcgagcgttg ttcggaatga ctgggcgtaa agggagcgca ggcggttcga
       61 tttgtccggc gtgaaagccc ggggctcaac cccggcgggc cgtcggaaac ggtcgaactc
      121 gagtacggaa gaggagagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccgatactga cgctcaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005375
VERSION     KHUT01005375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgag
       61 caagtctagg gtgaaatctt tcggcttaac cggaaaatag ccttggatac tgctggtctc
      121 gagggaggta ggggtcgttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aacgactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005376
VERSION     KHUT01005376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           98 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggcggac
       61 taagtcaggc gtgaaagccc tgggcttaac ctgggaattg cgtttgatac tggtttgctt
      121 gagtgcggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005377
VERSION     KHUT01005377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 tacgtaggga gctagcgtta ctcggaatca ctgggcgtaa agggcgtgta ggcggataag
       61 cgtgtctggt gtgaaatccc tcggctcaac tggggaagtg cattagaaac tgcttgtctg
      121 gagtgcggga ggggagagtg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg gctctctggc ccgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005378
VERSION     KHUT01005378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatagg
       61 aaagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccgttgatac tatttatctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacatg
      181 gaacaccaat tgcgaaggca gcttactacg caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005379
VERSION     KHUT01005379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           86 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtctgat gtgaaatccc gcagctcaac tgcggaactg catcggatac tacctcgcta
      121 gagggtttga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga aaactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005380
VERSION     KHUT01005380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c          101 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggcggttggg
       61 taagtggtcg gtgaaagcgc ctggcttaac caggcgagtg ccggtcagac tgctcgacta
      121 gagtatcgga gagggacgtg gaattccggg tgtagcggtg aaatgtgcag agatccggag
      181 gaacaccagc gccgaaaggg gcgtcctgga cgaaaactga cgctgagacg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005381
VERSION     KHUT01005381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattga
       61 caagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgtcagtcta
      121 gagtccggaa gaggtgggtg gaattcctag tgtagaggtg aaattcgcag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactggt ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005382
VERSION     KHUT01005382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          106 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggccgcg
       61 tatgtgaccg gtgacagcct cgggctcaac tcgaggtggt ctggttacac agcgcggctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005383
VERSION     KHUT01005383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           86 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ttgggcgtaa agcgcgcgca ggcggattaa
       61 taagtcaggt gtgaaatctc gaggctcaac ctcgaaactg cgcctgaaac tattagtcta
      121 gaatttcgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggg ggcgaaggcg cctacctaga cgaatattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005384
VERSION     KHUT01005384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           90 g           46 t
ORIGIN      
        1 tacgtaagtg gcaagcgttg tccggaatca ctgggcgtaa agcgcatgta ggcggctttg
       61 taagtccggg gtgaaatcca gaggctcaac ctctgaactg ccttggaaac tgcaaagcta
      121 gaggtacgga gggggaagcg gaattccagg tgtagcggtg aaatgcgcag atatctggga
      181 gaacaccggt ggcgaaggcg gcttcctggc cgttacctga cgctgagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005385
VERSION     KHUT01005385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           75 g           65 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttga
       61 taagtcagtg gtgaaatctt tcggctcaac cgagaaatgg ccattgatac tgtcagactt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005386
VERSION     KHUT01005386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           89 g           47 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgtt
       61 caggtcaggt gtgaaatccc ccggcccaac cggggaatgg cgcttgaaac cgttcggctt
      121 gagtgaccta gaggtgcatg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccaga ggcgaaagcg atgcactggg ggttaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005387
VERSION     KHUT01005387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgaggggg gctagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggagcc
       61 taagttgggt gtttaattcc ggggcccaac cccgggcgtg cactcaaaac tgggtttctt
      121 gaatagggga gaggtcggtg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacacccgt tgcgaaggcg gccgactgga cccatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005388
VERSION     KHUT01005388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c          101 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttggg
       61 taagtggcgt gtgaaagctc ccggctaaac tgggagagga caggtcagac tgccgaactg
      121 gagtccgata gaggggcgta gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaataccagt ggcgcaagcg gcgccctggg tcggtactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005389
VERSION     KHUT01005389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggccgg
       61 caagtcaggt gtgaaatccc cgggctcaac tcgggaactg cacttgaaac tgccggtctg
      121 gagtgccgga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005390
VERSION     KHUT01005390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           93 g           44 t
ORIGIN      
        1 aacgtaggtc ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtgggc
       61 taagtcaggc atgaaatctc acggctcaac cgagagaggc tgtctgagac tggcacactt
      121 gagggcagtc gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaagcg gccacctggg ctgtacctga cgctgacacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005391
VERSION     KHUT01005391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 aacgtagggg acgagcgtta tccggattta ctgggcgtaa agcgcatgta ggtggcttga
       61 aaagttggac gtgaaagctc ctggcttaac tgggagaggt cgtacaaaac tagcaagctt
      121 gaggttggaa gaggagagca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gctctctggt tcaatactga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005392
VERSION     KHUT01005392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           86 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattca ttgggcgtaa agggtccgta ggcggttcag
       61 aaagttggat gtgaaatccc atggctcaac catggaactg cattcaaaac tcctggacta
      121 gaggatgaga ggggtagagg gaattcccgg tgtaagggtg aaatctgcag agatcgggaa
      181 gaacaccagt cgcgaaggcg ctctactggc tcatatctga cgctgaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005393
VERSION     KHUT01005393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           94 g           39 t
ORIGIN      
        1 gacagggggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggaccag
       61 aaagcatctt gtgaaagccc ccggctcaac cggggaattg cggggtgaac tcctggtctt
      121 gaggcaggta ggggcagacg gaactgtcgg tggagcggtg aaatgcatag atatcgacag
      181 gaacgccaat ggcgaaggca gtctgctggg cctgtcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005394
VERSION     KHUT01005394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          100 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 gcagtccgtg gtgaaagctc agggctcaac tctgagtcgg ccacggatac tctcgtgctc
      121 gagtctggta gaggctcatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgagctggg ccagtactga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005395
VERSION     KHUT01005395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catctcatac tgagagactg
      121 gagtgctgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005396
VERSION     KHUT01005396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           64 c          110 g           35 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggctgtg
       61 cgagcccggc gtgaaagccc ccggctcaac cgggggaggt cgtcggggac cgcacggcta
      121 gaggccggta ggggcgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01005397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005397
VERSION     KHUT01005397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgat
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg catatgatac tgatcagcta
      121 gagtacagaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggt ctgttactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005398
VERSION     KHUT01005398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgca ggctgttttg
       61 tgtgtctgct gtaaaagcac acggctcaac tgtgtaaagt cggtagaaac tgcaaaactt
      121 gaggctggga gaggctggtg gaattccctg tgtagcggtg aaatgcgtag atatagggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005399
VERSION     KHUT01005399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          100 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggactcg
       61 taagtctgtg gtgaaagccc ccagctcaac tggggaaggg ccttggatac tgcgagtctg
      121 gaggagggta ggggcatctg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005400
VERSION     KHUT01005400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           79 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggttgaccgg
       61 aaagtcagtg gtgaaatact ctcgcttaac gagagaactg ccgttgatac tatcggtctt
      121 gaatacggtg gaggcgggcg gaatgtgtca tgtagtagtg aaatacttag atatgacaca
      181 gaacacctat tgcgaaggca gctcgctacg ccgtcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005401
VERSION     KHUT01005401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           95 g           42 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgca
       61 caagtccgac gtgaaatccc acggctcaac cgtggaactg cgtcggaaac taggtggctt
      121 gaatccaaga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ttggcattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005402
VERSION     KHUT01005402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          100 g           40 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ctcggagtta ctgggcataa agggcgcgta ggcggtcgtg
       61 cacgtcgagg gtgaaatccc cgggcttaac ccgggaactg cctttgatac ggcgcgactc
      121 gagtgcggga ggggagagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggt ccgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005403
VERSION     KHUT01005403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           96 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgct gtgaaagccc agggcttaac cctgggtttg cagtgggtac gggcaggcta
      121 gagtgcagta ggggagaccg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01005404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005404
VERSION     KHUT01005404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c          105 g           34 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggccgcg
       61 caagtcgggc gtgaaagccc acggctcagc cgtggaaggg cgctcgaaac ggcgcggcta
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005405
VERSION     KHUT01005405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggattca ttgggcttaa agcgtacgta ggcggatatg
       61 caggcgtcgt gtgaaagccc tcggctcaac cgaggaattg cacggcgaac cgcatgtctt
      121 gagatcggta ggggctgtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggcagctggg ccgattctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005406
VERSION     KHUT01005406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtccgt
       61 taagtctgat gtgaaagccc ggggctcacc cccggaagtg cattggaaac tggcggactc
      121 gagtcccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggtgactga cgctaatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005407
VERSION     KHUT01005407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgca ggcggtcttt
       61 taagtccact gttaaatccc ggggctcaac cccggagatg cagtggatac tgagagactg
      121 gagaccggta gaggctagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctagctggg ccggttctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005408
VERSION     KHUT01005408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggcttat
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tgatgagctt
      121 gagtccgaga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005409
VERSION     KHUT01005409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 caagtctggt gtgaaatccc ggggctcaac ctcggaactg cactggatac taccttgcta
      121 gagtactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagtaactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005410
VERSION     KHUT01005410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgactact
       61 taagttttgt gtgaaatccc aaggcctaac cttggaattg cataagatac tgagtggatt
      121 gagttcagga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005411
VERSION     KHUT01005411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ctgggcgtaa agggcgcgta ggcggcgtgg
       61 caagtccaag gtgaaatccc tcggctcaac cgaggaattg ccttggaaac tgcctcgcta
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005412
VERSION     KHUT01005412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           86 g           51 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggcgcat
       61 taagttcgtt gttaaaggct ccagcctaac tggagtaccg cgatgaaaac tggtgtgcta
      121 gagggtgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005413
VERSION     KHUT01005413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           94 g           41 t
ORIGIN      
        1 tacggggggt gcgaacgttg ttcggaatca ctgggcgtaa agagcgcgta ggcggcctca
       61 taagtggagt gtgaaatcca ccggctcaac cggtgaactg caccccaaac tgcgaggctt
      121 gagggcagca gaggaaacgg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg cgtttctggg ctgcatctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005414             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005414
VERSION     KHUT01005414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggttcat
       61 ttagttcggg gtgaaaggtt caacgctcaa cgtgaacagt gccttgaata ctggtggact
      121 tgagttcggg agagggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctgcctgg cctagaactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005415             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005415
VERSION     KHUT01005415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           97 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggattgt
       61 acagtcaggt gtgaaattcc cgggcttaac ctgggggctg cacttgatac gtaggatctg
      121 gagtgaggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttttactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005416
VERSION     KHUT01005416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccgc
       61 taagttcggc gtgaaatccc ctggctcaac tggggaactg cgctggatac tggtggactc
      121 gagtaccgga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga cggatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005417
VERSION     KHUT01005417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctcgc
       61 caagtcagat gtgaaagccc cgggcttaac ctgggaattg cgtttgaaac tggcgggctg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005418
VERSION     KHUT01005418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggaggttgga
       61 taagtctctt gtgaaagctc ccggcttaac tgggagaggt caagggaaac tgttcggcta
      121 gagtgtggta gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca gtcttctgga ccatcactga ctctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005419
VERSION     KHUT01005419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           90 g           43 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggagcgt
       61 taagtgcctt gtgaaatccc gcggctcaac cgcggaactg ctgggcatac tggcgctctt
      121 gagccaatca ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgaa ggcgaaagca gacgactggg attgcgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005420
VERSION     KHUT01005420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          104 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 agagtcaacg gtgaaagctc ggggctcaac cccgagtcgg ccgtcgatac ttccgggctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005421
VERSION     KHUT01005421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           49 c           82 g           48 t
ORIGIN      
        1 tacgtaggat gcgagcgtta tccggaatta ttgggcgtaa agcgtccgca ggggggaatg
       61 taagtctctt atcaaatccc gatgctcaac atcggaccgg taagggaaac tacatttcta
      121 gaggatggga gaggctagtg gaattcccgg tgtaggggta aaatccgtag atatcgggag
      181 gaacaccaaa agcgaaggca gctagctgga acattcctga ccctcaggga cgaaagcgtg
      241 gggagcgaaa agg
//
LOCUS       KHUT01005422             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005422
VERSION     KHUT01005422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          100 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcggctttc
       61 taagtctggt gtgaaaggtt cagggctcaa cccgaacagt gcactggata ctggcaggct
      121 tgagtgcggc agaggggggc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agccccctgg gccgtttctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005423             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005423
VERSION     KHUT01005423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           65 c          102 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtcggtc gctgaaatcc cccggctcaa ccggggaagg ggcgccgata cgaccggcct
      121 ggaggggcgt agggggatcg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gcggtcctgg acgcctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005424             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005424
VERSION     KHUT01005424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           53 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggttttg
       61 taagtcggat gtgaaaactc caagctcaac ttggagcctg catccgaaac tgcgatgact
      121 tgagtctggt aggggaacac ggaatacctg gtgtagcggt gaaatgctta gatatcaggt
      181 ggaacaccag tggcgaaggc ggtgttctgg gccagtactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005425             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005425
VERSION     KHUT01005425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           62 c          108 g           36 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggagtga ttgggcgtac agcgcgcgta ggcgggctcg
       61 atcgtcgtgc gtgaaaggtc ggggctcaac cccgacacgt cgcccgagac atcgggtctt
      121 gagcccggca gggggtggtg gaattccgcg aggagtggtg aaatgcgttg agacgcggag
      181 gaacaccggt ggcgaaggcg gccacctggg ccgttggctg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005426             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005426
VERSION     KHUT01005426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggtttgg
       61 caagtttgac gtgaaagccc cgggcttaac ccgggaatgg cgttgaaaac tgcttgaact
      121 ggagggtcgg aggggggact ggaattcttg gtgtagcagt gaaatgcgta gatatcaaga
      181 ggaacaccag tggcgaaggc gagtccctgg acgatacctg acactgaggc acgaaagcca
      241 agggagcaaa cagg
//
LOCUS       KHUT01005427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005427
VERSION     KHUT01005427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccca
       61 taagtctgcg gtgaaaccca cgggctcaac ccgtggcgtg ccgacgatac tgtggggctt
      121 gggtcgtctc tgggcgggcg gaatggaagg tgtagcggtg aaatgcatag atatctacca
      181 gaacgccgat agcgaaggca gctcgctggg gacgtaccga cgctgaggca cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005428
VERSION     KHUT01005428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           86 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagcgta ggttgtgtcg
       61 caagtcagtg gtgaaatccc agagcttaac tctggaactg ccattgaaac tgcgatgcta
      121 gaatctcaga gggggtagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctacctgga tgagtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005429
VERSION     KHUT01005429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           42 c           78 g           50 t
ORIGIN      
        1 gacgggggat gcaagtgtta tccggaataa ttgggcgtaa agcgtctgca aatggtttat
       61 caagtctgtt gttaaaaatc agggcttaac cctgatccgg caacagaaac taataagcta
      121 gagtacggta ggggcagagg gaattctcgg tgtagtggtg aaatacgtag atatcgagaa
      181 gaacaccaat agcgaaagca ctctgctggg ccgaaactga catggagaga cgaaagctag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01005430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005430
VERSION     KHUT01005430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           85 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttatt
       61 taagttagat gtgaaatccc cgggcttaac ctgggaattg catttaagac tgaaagacta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga ctcatactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005431
VERSION     KHUT01005431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgagaa gtgaaagctg tgggctcaac ccacaaaccg cttttcatac tggcgagctg
      121 gagtgatgca gaggtggata gaattcccgg tgtagcggtg aaatgcgttg atatcgggag
      181 gaatacccgt ggcgaaggcg gtccactggg catcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005432
VERSION     KHUT01005432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           88 g           49 t
ORIGIN      
        1 cacggaccgc acgaacgtta ttcggtatca ctgggcttaa agggtgcgta ggcggctttg
       61 caagttgggt gtgaaagccc tcggctcaac cgaggaattg cgctcaatac tgccgagctc
      121 gagagagaca gaggtaagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaacaccggt ggcgaaggcg gcttactggg tcttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005433
VERSION     KHUT01005433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggttggtaga
       61 tgtgttggct gtgaaatccc cgagctcaac ttggggcggt cagccaaaac tgtctatcta
      121 gagtatggta gaggaaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaggcg actttctgga ccaatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005434
VERSION     KHUT01005434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           79 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctatt
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaattg ccattgatac tggatagctt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacacctat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005435
VERSION     KHUT01005435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           76 g           61 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattta ttgggtttaa agggagcgta ggtggcctta
       61 taagtcagcg gtgaaagact agagcttaac tctggaattg ccattgatac tgtagggctt
      121 gagtatagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacttag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctataactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01005436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005436
VERSION     KHUT01005436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtttcg
       61 taagtcccgg gtgaaagccc tcggctcaac cgaggaattg ccctggatac tgcgaagctc
      121 gaggctggaa gagggtagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggg ccagatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005437
VERSION     KHUT01005437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           88 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctga
       61 taagtcagcg gtgaaagttt ccggctcaac cgggagactg ccgttgatac tgtcaggctt
      121 gagagacgga ggggtacatg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcgaaggca ttgtaccggc cgttttctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005438
VERSION     KHUT01005438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtgg
       61 taagtggggt gtgaaagccc tcagctcaac tgaggaacag cgctccaaac taccatgctc
      121 gagggagaca gaggtcagtg gaacttcggg tggagcggtg aaatgtgttg atatccgaag
      181 gaacaccggt ggcgaaagcg actgactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005439
VERSION     KHUT01005439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccgtt
       61 caagtcgaac gtgaaatccc tcggctcaac cggggaactg cgtccgatac tgtgcggctt
      121 gagtcctgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga caggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005440
VERSION     KHUT01005440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgtgg
       61 caagtctgat gtgaaatccc ggggctcaac cccggaactg cattggatac tgccatgctt
      121 gagcactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttgctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005441
VERSION     KHUT01005441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttag
       61 taagtcaaat gtgaaagccc tgggcttaac ctaggaatcg catatgatac tgctagactt
      121 gagtatcgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005442
VERSION     KHUT01005442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           80 g           58 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactga
       61 taagtcagga gtgaaatctc ggcgcttaac gccgaaacgt cttttgatac tgtcggtctt
      121 gaattagcga gagattcgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcgaattggc gctatattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01005443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005443
VERSION     KHUT01005443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           42 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggtggtacga
       61 caagtctgct gtgaaagcgc ctggcttaac cgggcgaggt cggcggaaac tgtcgcactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccgctcctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005444
VERSION     KHUT01005444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggccaat
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catacaagac tggttggcta
      121 gagtgcagta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctatctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005445
VERSION     KHUT01005445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccaa
       61 taagtcagat gtgaaagtcc tgggcttaac ccagggattg catttgaaac tgttgggctt
      121 gaggacagga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtccctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005446
VERSION     KHUT01005446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtccggg gtgaaagccc gttgctcaac aacggaactg ccctggatac tggcgggctt
      121 gagtatagtg gaggttggcg gaatggaccg agtagcggtg aaatgcatag atacggtcca
      181 gaaccccgat tgcgaaggca gctgactaca ctactactga cgctgaggca cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005447
VERSION     KHUT01005447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacgtg
       61 taagtcaggt gtgaaatccc cgggctcaac ccgggaactg cacttgaaac tgcgcgtctt
      121 gagtgctgga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005448
VERSION     KHUT01005448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          100 g           46 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggcttgt
       61 tgcgtcggct gtgaaaaccc gcggctcaac cgcgggcttg cagccgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgagaag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005449
VERSION     KHUT01005449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtcaggt gtgaaagccc tcggctcaac cggggaagtg cacttgaaac taccgagcta
      121 gagtcccgga gaggatggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005450
VERSION     KHUT01005450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           98 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggtctct
       61 taagtctgat gtgaaagccc ccggcttaac cggggagggt cattggaaac tgggagactt
      121 gagtgcagga gagggaagcg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005451
VERSION     KHUT01005451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           94 g           51 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggttctg
       61 tgtgtcggat gtgaaatccc acggattatc cgtggaactg cattcgaaac tgcagggctg
      121 gagtgcagga gaggaaggtg gaattctcgg tgtagcggtg aaatgcgttg atatcgagag
      181 gaacgccgga ggcgaaggcg accttctgga ctgcaactga cgctgaggca cgaaagtcag
      241 gggagcaaac agg
//
LOCUS       KHUT01005452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005452
VERSION     KHUT01005452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           85 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggcgggg
       61 aaagtttaag gtgaaatctc caggcttaac ttggaaaatg cctcgaatac ttccctgctt
      121 gagggtcgca gaggagattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacatcagt ggcgaaagcg aatctctggg cgactcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005453
VERSION     KHUT01005453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccac
       61 caagtccggg gtgaaatcct tcggcttaac cggagaattg cctgggatac tggtggtctt
      121 gagggaggta ggggcacgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005454
VERSION     KHUT01005454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           86 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttagg
       61 taagtccgat gtgaaatctc caggctcaac ctggaaatgg cattggatac tatttagctt
      121 gaggagtgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cacttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005455
VERSION     KHUT01005455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctaca
       61 taagacagat gtgaaagccc cgggcttaac ctgggaactg cgtttgtgac tgtgtggcta
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ttgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005456
VERSION     KHUT01005456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           86 g           50 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggtggttaga
       61 aaagtcaggt gtgaaatccc ccagctcaac tggggaatgg cgcttgaaac tctctgactg
      121 gagtgagcta ggggtacttg gaactgccag tggagcggtg aaatgtgttg atattggcag
      181 gaacaccgga gccgaaaggg aagtactggg gcttaactga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005457
VERSION     KHUT01005457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           84 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtagtt
       61 taagttagat gtgaaatccc agggcttaac cctggaatag catttaagac tgaattgcta
      121 gagttttgta gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttactgga cagagactga cactgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005458
VERSION     KHUT01005458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 aaagttcggt gtgaaagctc caagctcaac ttggaaatgt cattgaaaac ttcccggctt
      121 gaggattgaa agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctggg caattcctga cactgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005459
VERSION     KHUT01005459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgaaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggccatg
       61 taagttgggg gtgaaatccc ggggctcaac cccggaactg ccttcaaaac tacatagctg
      121 gaggatgtga gaggatagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccggt ggcgaaggcg actgtctggc acatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005460
VERSION     KHUT01005460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           84 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagccg acagctcaac tgtcgaactg ccattgatac tggtaacctt
      121 gaattcagtt gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat tgcgaaggca gcttactggg cttgaattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01005461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005461
VERSION     KHUT01005461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           86 g           55 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtctgta ggcggtctgt
       61 taagtgaggt gttaaatcgt gtggctcaac catatcgttg tgcctcatac tggcagacta
      121 gaggaaggca gaggcaagtg gaattggcag tgtagcagtg aaatgcgtag agattgccag
      181 gaacaccaat ggcgaaggca gcttgctggg ccttttctga cgctgagaga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005462
VERSION     KHUT01005462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtttga
       61 caagtccgcc gtgaaagtcc agggctcaac cctgggatgc cggtggaaac tgtctggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005463
VERSION     KHUT01005463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           87 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggcttt
       61 taagtcagtg gtgaaatcct tcggctcaac cggagaactg ccattgatac tgggagtctt
      121 gagttcagtt gaggtaagcg gaattgatgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat tgcgtaggca gcttactggg cttggactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01005464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005464
VERSION     KHUT01005464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           88 g           46 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccatac tcccgtgcta
      121 gaggaggcta gaggtaagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gcttactggg acctttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005465
VERSION     KHUT01005465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          102 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgagta ggcggctttt
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tggaaggctt
      121 gagcacggta gaggcacatg gaattccggg tgtagcggtg gaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gtgtgctggg ccgtagctga cgctgaatcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005466
VERSION     KHUT01005466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcacgta ggcggcccga
       61 taagtccgtt gtgaaagtca gaggctcaac ctctggaggc caatggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005467             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005467
VERSION     KHUT01005467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           53 c          103 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa aacgcttgta ggcggccacg
       61 ctgcgtcgtg cgtgaaatct cccggcttaa ctgggagggg tcgtgcgata cgagcgggct
      121 cgaggcaggt agggggaagc ggaattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggcttcctgg acctgatctg acgctgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01005468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005468
VERSION     KHUT01005468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcgcgg
       61 taagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgctgtgctt
      121 gagtgtcgga gagggtcgcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gcgacctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005469             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005469
VERSION     KHUT01005469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           88 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgc
       61 taagtcggat gtgaaaactc ggggctcaac cccgagacgc catccgatac tggcgtgact
      121 tgagtctggt aggggagatg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc acatctctgg gccagtactg acactgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005470
VERSION     KHUT01005470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           92 g           52 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgg
       61 taagtcgggg gtgaaatcct gcagcttaac tgtagaagtg ccttcgatac tgccagtctg
      121 gagggagata ggggtgtgtg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg acgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005471
VERSION     KHUT01005471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtctgct gtgaaaactc gaggctcaac ttcgagatgt cggcggaaac catttggcta
      121 gagtccggaa gaggagagta gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaatacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005472
VERSION     KHUT01005472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggtcaga
       61 taagtccggt gtaaaagtca caggctcaac ctgtgaaagc cgctggaaac tgtctggctt
      121 gagtccggga ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctgga acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005473
VERSION     KHUT01005473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggtggac
       61 ccagtccggg gtgaaatctt tcggctcaac cggaaaagtg cctcggatac tggttcactc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005474             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005474
VERSION     KHUT01005474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           97 g           42 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccaga
       61 ccaagtcgag tgttaaattc cagggcttaa ccctggacag ccgctcgata ctggtcggct
      121 agagtgtgga agagggtgct ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc gggcacctgg gccaacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005475
VERSION     KHUT01005475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           99 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agagctcgta ggcggggctg
       61 cgcgtcgcgg agtaaatcca tctgcttaac ggatggctca tctgcgatac ggcagctctt
      121 gaggacagga gagggaagcg gaattccgag tgtagcggtg aaatgcgtag agattcggag
      181 gaacaccggt ggcgaaggcg gcttcctggc ctgattctga cgctgaggag cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005476
VERSION     KHUT01005476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcggag
       61 taagtcggat gtgaaatctc cgagctcaac tcggaaatgg cattggaaac tactctgctc
      121 gagggttgga ggggggactg gaatgcttgg tgtagcagtg aaatgcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg agtccctaga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005477
VERSION     KHUT01005477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttcgc
       61 taagttggat gtgaaagctc ccggctcaac tgggagaggt cgtacaagac tggcggactg
      121 gagtgtggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctaga ccacgactga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005478
VERSION     KHUT01005478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           80 g           55 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattta ttgggtgtaa agggtgtgta gacggtatat
       61 taagttcgct gttaaaactt tcggcctaac cggaagctcg cggtgaaaac tgataaacta
      121 gagggtgaaa gagaggagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaagca gctccttggt tcacacctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005479
VERSION     KHUT01005479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           94 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtttac
       61 caagtctgtt gtgaaatctc ccggctcaac tgggagggtg cggcggatac tggtgaacta
      121 gagtgcgaga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgaagacg gctctctgga tcgtgactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005480
VERSION     KHUT01005480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           79 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattgg
       61 taagtcagtg gtgaaatccc cgagcttaac tcgggaactg ccattgatac tattagtctt
      121 gaataccgtg gaggtcgacg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gttggctaca cgagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005481
VERSION     KHUT01005481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatca ctgggcttaa agcgttcgta ggcggacttg
       61 caggcgcgtt gtgaaagccc ccggctcaac cggggaactg cttcgcgaac cgcaagtctt
      121 gagtcaagta ggggcggtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccaaa ggtgaagaca ggccgctggg cttgtactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005482
VERSION     KHUT01005482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggttgcg
       61 tatgtctggg gtgaaatccc tgagcttaac tcaggacgtg ccttggaaac ggcgtaacta
      121 gagtactaga gaggttcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacatcagc ggcgaaggcg gcgaactgga tagtaactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005483
VERSION     KHUT01005483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgtgca ggcgggctag
       61 caagtggtgt atgaaagctc ccggctcaac cgggaagggt tatgccagac tgctagtctt
      121 gagtgcgaga gaggagcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgctctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005484
VERSION     KHUT01005484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcggat gtgaaatccc gaggctcaac ctcgggcctg cattcgatac gggctagcta
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005485
VERSION     KHUT01005485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggcgctg
       61 caagtgcctt gtgaaatccc atcgcttaac gatggaactg ctcggtagac tgcagtgctt
      121 gaggttggta ggggctgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcccaa ggcgaaggca ggcagctggg ccaattctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005486             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005486
VERSION     KHUT01005486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          109 g           35 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccccg
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tggggggctg
      121 gagccgatca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtcttggctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005487
VERSION     KHUT01005487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           93 g           54 t
ORIGIN      
        1 gacgtaggtg gcgagcgtta ttcggaatta ctaggcgtaa agcgagtgta ggcgggcgct
       61 taagtctgtt gtgaaatctc ccggcttaac tgggaggggt caacggaaac tgggtgtctt
      121 gagtgaggta gggggtactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaggca ggtacctggg cctttactga cgctgagact cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005488
VERSION     KHUT01005488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           89 g           56 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtctgat gttaaagact ggggctcaac cctgggaccg cattggatac tggcagactc
      121 gagtgcagta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaagcg acttgctggg ctgtaactga cgctgaggaa cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01005489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005489
VERSION     KHUT01005489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           94 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatga ctgggcgtaa agggcacgca ggtggtgact
       61 taagtgaggt gtgaaatccc cgggcttaac ctgggaacag catttcagac tgggtgacta
      121 gagtacttta gggaggggta gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccgaa ggcgaaggca gccccttggg aagatactga cgctcgagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005490
VERSION     KHUT01005490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattca ctgggtataa agggtgcgta ggcggcgcga
       61 taagtcaggg gtgaaatatg tcggctcaac cgacaaactg cctctgaaac tgccgcgctt
      121 gaggtcggga gaggaaggtg gaattccagg tgtagcggtg aaatgcgtaa atatctggag
      181 gaacaccaat ggcgaaggca gccttctggt ccgattctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005491
VERSION     KHUT01005491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 gacgaaccga gcaaacgtta ttcggaatta ctgggcttaa agagtccgta ggcggcgcgt
       61 taggtggggt gtgaaatccc tcggctcaac cgaggaattg cgctccaaac cggcgtgctt
      121 gaggaaggta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg ccttttctga cgctgaggga cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005492
VERSION     KHUT01005492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttcg
       61 caagtcgggt gtgaaatccc caagctcaac ttgggaactg cattcgagac tgcattgcta
      121 gagtatggga gagggcagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg actgcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005493
VERSION     KHUT01005493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          103 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcgcga
       61 taagtccatt gtgaaatccc ggggctcaac tccggggctg cagtggagac tgtcgtgctt
      121 gagcacagta ggggcgagcg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacatcggt ggcgaaggcg gctcgctggg ctgttgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005494
VERSION     KHUT01005494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           79 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggtggtttgg
       61 caagtcgaat gtgaaagccc ggcgctcaac gccgtaattg catacgatac tgctagactg
      121 gagttttgaa gaggggtcta gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaataccaat ggcgaaagca agaccctgga caaatactga cactgatgca cgaaggccaa
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005495
VERSION     KHUT01005495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          104 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcaagt
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tggctcgctc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005496
VERSION     KHUT01005496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           96 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgag
       61 tcagtctgat gtgaaagccc cgggcttaac ctgggaatgg cattggatac tgctgaactt
      121 gagtgtgata gaggaccgta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcgat ggcgaaggca gcggtctggg tcaacactga cgctgagggg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005497
VERSION     KHUT01005497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggttcgg
       61 caagttggat gtgaaatctc ccggctcaac tgggaggggt cgttcaaaac taccgaactt
      121 gagggcatca gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctggg atgctcctga cgctcagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005498
VERSION     KHUT01005498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcgggtctg
       61 tcagtctacc gtgaaagctt acggctcaac cgtaacgggt cggtggatac tgcagatctt
      121 gagggcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgttactga cgctgaggcg cgaaagctgg
      241 gggagtgaac ggg
//
LOCUS       KHUT01005499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005499
VERSION     KHUT01005499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtcgg
       61 tgtgtcgggt gtgaaatccc caggctcaac ctgggaactg cattcgaaac taccggccta
      121 gagtggtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gcttcctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005500
VERSION     KHUT01005500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcataa agcgcacgta ggcggttcgc
       61 taagtctgtc gtgaaatccc ccggctcaac cggggaatag cgtcggatac tggcgagctc
      121 gaggtcgata ggggtaagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactgga tcgaatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005501
VERSION     KHUT01005501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           86 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggatttg
       61 taagtcagat gtgaaatctc gaagctcaac ttcgaaactg cgtctgaaac tacaagtcta
      121 gaatgtcaga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggt ggcgaaggcg cctacctgga tgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005502
VERSION     KHUT01005502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtcgcg
       61 taagtcgggt gtgaaatccc gaggctcaac ctcggaactg cactcgatac tgcgtgactt
      121 gggtgctgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagctaccga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005503
VERSION     KHUT01005503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggtccgt
       61 taagtcgtgt gtgaaatgcc ccggctcaac cggggaattg cgcacgatac tggcgggcta
      121 gaggtcggga ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactggc ccgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005504             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005504
VERSION     KHUT01005504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          101 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccaga
       61 ccaagtcgag tgtgaaattg cagggcttaa ctttgcaggg tcgctcgata ctggtcggct
      121 agagtgtgga agagggtgct ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc gggcacctgg gccgacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005505
VERSION     KHUT01005505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacgaaggtc ccgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgtgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccgtgctt
      121 gagggttgga gaggtgactg gaattatcgg tgtagcagtg aaatgcgtgg atatcgatag
      181 gaacactcgt ggcgaaggcg agtcactgga caacatctga cgctgaggct cgaaggctag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01005506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005506
VERSION     KHUT01005506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           45 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcgcgg
       61 taagtgttct gtgaaatccc acggctcacc cgtggaactg cagggcatac tgccgtgctt
      121 gaggcaggta ggggtcactg gaactctcgg tggaacggtg aaatgtgtag atatcgagag
      181 gaacgcccga ggcgaaagcg ggtgactggg cctgtcctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005507
VERSION     KHUT01005507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctgtc
       61 cacgtcgggt gtgaaagctc agggctcaac cctgagatgc cattcgatac gggacggcta
      121 gaggtaggta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005508
VERSION     KHUT01005508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          100 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctg
       61 tgcgtcgggc gtgaaagccc tgggctcaac ccaggagtgg cgctcgatac ggcggagctc
      121 gagtgtggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005509
VERSION     KHUT01005509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agagcgcgca ggcggtttgg
       61 taagtcggat gtgaaatccc ggagctcaac tccgggcctg catccgatac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005510             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005510
VERSION     KHUT01005510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaacg gcttcgcata ctggcagact
      121 ggagggatgc agaggtgagt ggaattcctg gtgtaccggt gaaatgggta gatatcagga
      181 ggaacacccg tggcgaaggc ggctcactgg gcatctcctg acgctgaggc gcgaaagtgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005511             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005511
VERSION     KHUT01005511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           43 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgatac tgccgtggct
      121 agagtccggt agaggagcgt agaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaataccag cggcgaaggc ggcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005512             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005512
VERSION     KHUT01005512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           95 g           52 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggttgcg
       61 caagtctggt gtgaaatctt caccgcttaa cgggaagagg tcactggaaa ctgcgtaact
      121 cgagtgtggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacacctg tggcgaaggc ggcttgctgg gccatcactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01005513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005513
VERSION     KHUT01005513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           97 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctgtg
       61 cgcgtggggt gtgaaagctc ttggcttaac taagagaggc catcccatac ggcacggctc
      121 gagccatgca gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg catgtgctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005514
VERSION     KHUT01005514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgct
       61 cacgtcggat gtgaaagctt ggagctcaac tccaagaggc catccgatac ggaacggcta
      121 gaggcaggta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt agcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005515
VERSION     KHUT01005515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           75 g           65 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcttg
       61 taagtcagtg gtgaaatctc ccggctcaac cgggaaattg ccattgatac tgctagtctt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactatc aatatattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005516
VERSION     KHUT01005516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           83 g           55 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtcttcg
       61 caagtcttcg gtgaaatctc cgggcttaac tcggaaattg tcggagaaac tgcgaggata
      121 gagtttggta gaggcagacg gaattgaagg tgtagcggta aaatgcgttg atatcttcaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccaacactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005517
VERSION     KHUT01005517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacagagggt acaaacgttg ttcggaatca ctgggcttaa agcgcgcgta ggtggttcgg
       61 taagtgcgat gtgaaagccc ccggcctaac cggggaactg catcgcggac tgccgagctt
      121 gagagcggta ggggagacta gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaggcg agtctctggt ccgcttctga cacttaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005518
VERSION     KHUT01005518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c          102 g           37 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcacgt
       61 caagtacgca gtgaaatctc cctgctcaac ggggagggtg cagcgtagac tggcgagcta
      121 gaggacggta gaggggggta gaactgctgg tgtagcggtg aaatgcgtag agatcagcaa
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005519             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005519
VERSION     KHUT01005519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           97 g           47 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggatctg
       61 taagtctgac gtgaaatctc cactgctcaa cagggagagg tcgctggata ctgcagatct
      121 ggagttcggc agaggaccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gcctgaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01005520             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005520
VERSION     KHUT01005520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcgcgta ggcggcatcg
       61 taagttggag gtgaaatcct gacggctcaa ccgcagaatt gcccccaata ctgcgatgct
      121 tgagtgagat aggggcatgt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccgg tggcgaaagc gacatgctgg atcttttctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005521
VERSION     KHUT01005521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttgg
       61 taggtccgct gtgaaaactc gaggctcaac ctcgagaggt cggcggaaac cacttggcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005522
VERSION     KHUT01005522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          104 g           42 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggcaggc
       61 caagtctgcg gtgaaatcgc ccggctcaac tgggcggagt ccgtggaaac tggtcagctg
      121 gaggacggta gaggtgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gctcactggg ccggtcctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005523
VERSION     KHUT01005523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 gacggagggc gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggatcgg
       61 taagtcaggg gtgaaagcct ggggctcaac tccaggactg cctttgaaac tgccgttcta
      121 gagttcagga gaggaaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005524
VERSION     KHUT01005524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtcggat gtgaaatctc caggctcaac ctggaaatgg cattggaaac taccttgctg
      121 gagagttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caacttctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005525
VERSION     KHUT01005525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaatccc tcagcttaac tgaggaagtg cgcccgaaac tgccgagctt
      121 gaataccgga gaggatggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccatctgga cggctattga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005526
VERSION     KHUT01005526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggacctg
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctttgatac tgtgggtctt
      121 gagttcgaga gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggc tcgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005527
VERSION     KHUT01005527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agagaacgta ggcggttcct
       61 taagtttggg gttaaagacc gaggctcaac ctcgggagtg ccctgaatac tgggggactg
      121 gaggacggca gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg actttctggg ccgttcctga cgctgaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005528             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005528
VERSION     KHUT01005528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcga
       61 ttagtcgggt gtgaaattcc gaggctcaac ctcggaacgc cactcgatac tgtcgtggct
      121 agagtccggt agaggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005529             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005529
VERSION     KHUT01005529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           51 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtccgt
       61 taagtgtgaa gtgaaatctc cagagctcaa ctcggaaact gctttacata ctggcggact
      121 tgaggaatgc agaggtgaat agaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaataccca tggcgaaggc agttcactgg gcattatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005530
VERSION     KHUT01005530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          100 g           43 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccttg
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaaccg cactcgatac tgccgggcta
      121 gagtccggga ggggatggtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01005531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005531
VERSION     KHUT01005531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta gtcggaatta ctgggcgtaa agcgtgcgca ggcggttttt
       61 tatgtcagat gtgaaatccc cgggctcaac ctgggaattg cgtttgaaac tggaagactg
      121 gaatgtggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ccaacattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005532
VERSION     KHUT01005532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtctcg
       61 caagttgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccaaaac tacgagactt
      121 gagtaccgga gaggatagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005533
VERSION     KHUT01005533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggctgt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatcagtctt
      121 gaattttgtt gaggagggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctctctaag caaacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005534
VERSION     KHUT01005534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 caagtcaggg gtgaaatctt tcggcccaac cggaaaagtg cttctgatac tggctgtctc
      121 gagggaggta ggggcgcgtg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg acgcgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005535
VERSION     KHUT01005535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ttcggaatca ttgggcgtaa agggtgcgta gacggtttgt
       61 taagtctgtt gtttaatacc ccagcctaac tggggaaatg caatagaaac tggcatacta
      121 gagggtgaga gagaggagtg gaattcctgg agtagcggtc aaatgcgtag atctcaggag
      181 gaacaccgat ggcgaaggca gctccttggc tcatacctga cgttgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005536
VERSION     KHUT01005536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcgatt gtgaaagctc ggggcttaac cccgagtctg cagtcgatac gggctagcta
      121 gagtgtggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005537
VERSION     KHUT01005537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           87 g           56 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggctgat
       61 taagtctgtt gttaaagact ccggcttaac tggagaaatg cagtggaaac tgattggcta
      121 gaggatgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccgat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005538
VERSION     KHUT01005538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          104 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccga
       61 cgcgtccggg gtgaaatcct cccgctcaac gggagaacgg cctcggagac agtcggtctg
      121 gaggggggta ggggcaggcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gcctgctgga ccctcactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005539
VERSION     KHUT01005539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           89 g           55 t
ORIGIN      
        1 cacgtaggag actagcgtta ttcggattta ctgggcgtaa agcgcgtgca ggcggttcgg
       61 ttagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaatac tgccggactt
      121 gagagtggta gagggagttg gaattccggg tgtagtggta aaatgcgtag atatccggaa
      181 gaacaccagt ggcgaaagcg gactcctgga ccatttctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005540
VERSION     KHUT01005540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcagtg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcattgctt
      121 gagtaccgga gaggatggcg gaattcccga agtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccatctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005541
VERSION     KHUT01005541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           77 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggcctta
       61 taagtcagtg gtgaaatctc gcagcttaac tgtgaaattg ccattgatac tgtagggctt
      121 gagtacagat gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctgacgaaa ctgtaactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01005542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005542
VERSION     KHUT01005542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttttt
       61 caagtcaggt gtgaaatctt caggctcaac ctggaaatgg cgcttgaaac tgagagactt
      121 gaggcagcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg gctgaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005543
VERSION     KHUT01005543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           94 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtccct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggggggctt
      121 gagtgcagga gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg actctctggc ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005544
VERSION     KHUT01005544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           79 g           59 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggctaaa
       61 taagtcagtg gtgaaagcct acggcttaac cgtagaattg ccattgaaac tgtttggctt
      121 gaattcaatc gaggcaagtg gaacgcagca tgtagcggtg aaatgcttag atatgctgca
      181 gaacaccaat tgcgaaggca gcttgctagg ttgttattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005545
VERSION     KHUT01005545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c           99 g           42 t
ORIGIN      
        1 tacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggctcgt
       61 caagcccgaa gtgaaatgtt ccggcccaac cgggaacgtg cttcggggac tggcgggctt
      121 gggcgagcta ggggtctgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acagactggg gcttggccga cgctcatgca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005546
VERSION     KHUT01005546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatca ctgggcgtaa agggtctgta ggcggttact
       61 taggtctggt gtgacacctc acggcttaac tgtgagtatg cattggaaac cgggtgacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005547
VERSION     KHUT01005547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtccgat gtgaaagccc tgggctcaac ctgggaatgg cattggaaac tggcgagctc
      121 gagttcggta gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gcgacctgga ccagaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005548
VERSION     KHUT01005548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           98 g           40 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggcagtt
       61 taagttcggc gtgaaagctc ctggctaaac cgggagaggt cgtcggaaac tggacagcta
      121 gagggtgata gaggcgggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acccgctggg tcacacctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005549
VERSION     KHUT01005549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           92 g           45 t
ORIGIN      
        1 gacggagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggtcagc
       61 ttagttggat gtgaaagccc ccggctcaac cgaggaattg cattcaatac tggctggcta
      121 gaggatggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccattactga cgctgaggtg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005550
VERSION     KHUT01005550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           99 g           41 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggaatta ctgggcgtaa agcgcgagta ggcggttgga
       61 taagtggtct gtgaaagcgt ccggctcaac tggacgaggc cggatcagac tgttcgacta
      121 gagggtcaga gaggcaggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gcctgctggc tgacaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005551
VERSION     KHUT01005551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           86 g           49 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 caagttggat gtgaaatccc tcagcttaac tgaggaactg cgttcaaaac tgccgtgctc
      121 gagggagata gaggagtgcg gaacttacgg tggagcggtg aaatgcgttg atatcgtagg
      181 gaacaccggt ggcgaaagcg gcactctgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005552
VERSION     KHUT01005552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          110 g           37 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggttgcc
       61 cgcgtcgcac gtgaaagccc ccggctcaac cggggagggt cgtgcgaaac ggggtgactg
      121 gaggcagtga gaggctggtg gaattcccgg agtagtggtg aaatgcgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gccagctggc actgacctga cgctgaggcg cgaaggcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005553
VERSION     KHUT01005553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           46 c           95 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggttttt
       61 taagtctagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggagaactt
      121 gagggatgca gaggaaagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggg catttcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005554
VERSION     KHUT01005554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattta ctgggcgtaa agggtgcgca ggcggccgga
       61 tgtgtcgggt gtgaaatttc gaggctcaac ctcgaaactg cgctcgaaac tgttcggcta
      121 gagttctgga gagggaggcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaat ggcgaaggca gcctcctgga cagatactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01005555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005555
VERSION     KHUT01005555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagttgggg gtgaaatcct ggagctcaac tccagaactg ccttcaatac tggcaatctc
      121 gagtccggaa gaggtaagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005556
VERSION     KHUT01005556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggcgttg
       61 taagtgcggt gtgaaagccc acggcttacc cgtggaacag caccgtagac tgcaatgcta
      121 gaggatggta gaggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccgtttctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005557
VERSION     KHUT01005557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           97 g           45 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agagcgcgta ggcggtttga
       61 taagtctgct atgaaagcac ctggctaaac tgggtgaggc tggcggagac tgtcaggcta
      121 gagtgagaca gaggcagatg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gtctgctggg tctcaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005558
VERSION     KHUT01005558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           89 g           47 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatccg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgcgggtctt
      121 gagccaccta ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005559
VERSION     KHUT01005559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           98 g           41 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agggtgcgta ggcggcatgg
       61 caagtgaggc gtgaaagccc cgggctaaac tcggggagga catctcagac tgctaagcta
      121 gagtgaaaca gaggagcgcg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggtgcaaacg gcgctctggg tttagactga cgctgaggca cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005560
VERSION     KHUT01005560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           76 g           58 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggagcgta ggcggatctt
       61 taagtcagtg gtgaaagcct ggagcttaac tccagaattg ccattgaaac tgaagatctc
      121 gagtatggtt gaggttactg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccaat tgcgaaggca ggtaactaag ccataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005561
VERSION     KHUT01005561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c          100 g           38 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcacgca ggcggactag
       61 taagtgggtc gtgaaagcgc ccggctaaac cgggcgagga cgattcagac tgccggtcta
      121 gagtcggata gaggagcgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggcgcaagcg gcgctctggg tccgaactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005562
VERSION     KHUT01005562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcgggttgc
       61 taagtcgggg gtgaaatccc aaggctcaac cttggaactg ccttcgatac tggcaatctt
      121 gagtccggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005563
VERSION     KHUT01005563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggctttc
       61 taagtctaat gtgaaatccc ggggctcaac cccggaactg catcggatac tggaaagcta
      121 gaggatgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gccatctggc tcatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005564
VERSION     KHUT01005564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           87 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcatgta ggcggcacga
       61 taagttcact gtgaaagccc ttggctcaac caaggaagtg cagtgaaaac tgtcgagctc
      121 gagttctgaa gagggtcgcg gaatggccag tgtagaggtg aaattcgtag atattggcta
      181 gaacaccagt ggcgaaggcg gcgacctggg aagatactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005565             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005565
VERSION     KHUT01005565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           94 g           50 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatta ctgggcataa agcgcgcgta ggcggccacg
       61 taagtgtctt gtgaaatccc acggctcacc cgtgtgaatt gtgaggcata ctgcgtggct
      121 agagtgtggt aggggagagc ggaactcctg gtggagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggctctctgg tccactactg acgctgaggt gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01005566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005566
VERSION     KHUT01005566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtcact
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg catttgaaac tgagtggcta
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc ccattactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005567             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005567
VERSION     KHUT01005567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           79 g           56 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttactg gtggcaaatc ggggctcaac cccgtggaag tcagtaatac tatcgaacta
      121 gagggtcgga gaggttaacg gaactccatg tgtagcggta aaatgctcaa atatatggat
      181 gaacaccaat cggcgaaggc agttaactat tcgattcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01005568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005568
VERSION     KHUT01005568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          100 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcgga
       61 taagggggtg gtgaaatctc tgagcttaac tcagagccgg ccatcccgac tgttcggctc
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctttctgga ccactactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005569
VERSION     KHUT01005569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttcg
       61 taagcttgat gtgaaagccc cgggcttaac ctgggaatgg cattgaggac tgcgaggctg
      121 gagtgtggca gaggagactg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca ggtctctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005570
VERSION     KHUT01005570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           83 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggtgtgta ggcggaattt
       61 taagtagaaa gtgaaaggtt ggagctcaac tccaacactg cttcctatac tggaattctt
      121 gagtcccgga gaggaagatg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gtcttctgga cgggtactga cgctgagaca cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01005571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005571
VERSION     KHUT01005571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           94 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccagg
       61 caagtcgggt gtgaaagccc tgggcttaac ccaggaagtg cactcgaaac tgcccggctc
      121 gagtacggga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg accacctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005572
VERSION     KHUT01005572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgtagggg ccaagcgtta tccggattta ctgggcgtaa agcgcatgta ggtggtctgc
       61 taagtcggac gtgaaagctc ctggctcaac tgggagaggt cgttcgaaac tagtagacta
      121 gaggatggta gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggc ccattcctga cgctgagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01005573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005573
VERSION     KHUT01005573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatta ttgggtgtaa agggtgtgca ggcggggcaa
       61 acagtcaggg gtgaaagccc gcggctcaac cgtggaactg cctttgatac tgttgtcctt
      121 gagtgctgga gaggccactg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccggt tgcgtaggcg ggtggctgga cagcaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005574
VERSION     KHUT01005574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           88 g           55 t
ORIGIN      
        1 cacgtaggtg gcaagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggttaat
       61 caagttcggc gtgaaatctc tcggctcaac cgggaggggt cgttgaaaac tgattagctt
      121 gaggcaatga gagggatgca gaattcctgg tgtagtggtg gaatgcgtag atatcaggag
      181 gaataccagt ggcgaaagcg gcatcctggc attgttctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005575             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005575
VERSION     KHUT01005575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           84 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttgt
       61 gtagtcccat gtcaaagacc aggacttaac cctgggccgg catgtggaaa ctcgcaagct
      121 tgagtatggt aggggcagag ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 agaacaccaa cggcgaaagc actctgctgg gccaaaactg acactaagag acgaaagcta
      241 gggtagcaac tggg
//
LOCUS       KHUT01005576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005576
VERSION     KHUT01005576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggcttcg
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcaaggctc
      121 gagtgctgga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctctctgga cagttactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005577
VERSION     KHUT01005577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtgggaact
       61 taagtcaagt gtgaaatccc caggctcaac ctgggacgtg cacctgaaac tgggtttctt
      121 gagtacttga gagggtggtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaggcg accacctggc aagtcactga cactgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005578
VERSION     KHUT01005578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           92 g           43 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggcgggg
       61 taagttgggt gtgaaatctc ccggctcaac tgggagaggc cgctcaaaac tgcctggcta
      121 gaggacggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaagcg gccttctgga ccgtacctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005579
VERSION     KHUT01005579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggtgtaa agcgcatgca ggtggctgtg
       61 caagtatgct gtgaaagccc cgtgcttaac acggggaggc cagtatagac tgcacggctc
      121 gaggactgca gagggtaggg gaattgctgg tgtagtggtg aaatgcgtag agatcagcag
      181 gaacaccaag gccgaaggga cctacctggg cagtatctga cactcaagtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005580
VERSION     KHUT01005580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           80 g           61 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggcctgg
       61 taagtcagtg gtgaaagttt tcggcttaac cggaaaattg ccattgatac tgtcgggctt
      121 gaattcagat gaggtaggcg gaatgtgtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca gcttactaaa ctgagattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005581
VERSION     KHUT01005581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttga
       61 caagtcggat gtgaaatccc cgggcttaac ccgggaactg catccgaaac tgtttcgcta
      121 gagtctcgga gaggaaggca gaattcccag tgtaggggtg aaatccgtag atactgggag
      181 gaataccggc agcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005582
VERSION     KHUT01005582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           82 g           57 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggccgt
       61 taagtcaggg gtgaaagtct gcggctcaac cgtagaattg cccttgatac tggtggcctt
      121 gagttatggt gaagcagccg gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaacaccgat tgcgaaggca ggctgctaac catatactga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005583
VERSION     KHUT01005583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcctgg
       61 taagtttgat gtgaaagctc ggagcttaac tccgaaatgg cattgaatac tactgggctt
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005584
VERSION     KHUT01005584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgcgta ggcggcttga
       61 caagttggat gtgaaagccc tgggctcaac ccaggatgtg cattcaaaac tgtcgagctt
      121 gaatctcgga gggggtcagg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg ctgacctgga cgatgattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005585
VERSION     KHUT01005585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtttgt
       61 taagcaggat gtgaaatccc cgggctcaac ctgggaactg catcctgaac tggcaggcta
      121 gagtgtagta gagggtagtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga ctaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005586
VERSION     KHUT01005586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           48 c          102 g           50 t
ORIGIN      
        1 gacgtagggg gctagcgttg tccggattta ttgggcgtaa agagctcgta ggtggcttgt
       61 cgcgtcggtt gtgaaaacct gcagcttaac tgtgggcgtg cagccgatac gggcgggcta
      121 gaggcaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005587
VERSION     KHUT01005587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          108 g           37 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgca ggcggcccgc
       61 taagtccgct gtgaaagacc ggggctcaac cccggggtgt cagcggagac tggtgggctg
      121 gagtgtcgga gagggatgcg gaatacctgg tgtagcggtg gaatgcgtag agatcaggtg
      181 gaacaccgat ggcgaaggca gcatcctggc cggagactga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005588
VERSION     KHUT01005588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          104 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaagct
       61 taagtgtggc ctgaaagccc ccggctcaac cggggagggt ggctgcaaac tgggtttctt
      121 gagggggata gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg tccgccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005589
VERSION     KHUT01005589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggcccgt
       61 aaagtcaggt gtgaaattcc tgggctcaac ccaggacgtg cgcctgatac tggcgggctt
      121 gagtccggga gaggatggtg gaactcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacccgt ggcgaaggcg gccatctgga ccggtactga cgctaaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005590
VERSION     KHUT01005590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           90 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacccg
       61 caagtcggac gtgaaatccc tgagctcaac tcaggaactg catccgaaac tgcgggtctg
      121 gagtggtgga gagggaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005591
VERSION     KHUT01005591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggcggct
       61 caagccaggc gtgaaatccc cgagcttaac ttgggaatgg cgcttgggac tgggttgctt
      121 gagtgcggaa gagggagctg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacaccgat ggcgaaggca ggctcctggg ccagcactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005592             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005592
VERSION     KHUT01005592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           91 g           51 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcggat gtgaaaactc ggggctcaac cccgagacgc catccgatac tgctgtggct
      121 tgaatctggt aggggagatc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggatctctgg gccagtattg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005593
VERSION     KHUT01005593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           48 c          100 g           51 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcctgt
       61 tgcgtcaatc gtgaaaaccg gatgcttaac atctggcttg cggttgatac gggcgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005594
VERSION     KHUT01005594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           80 g           59 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgctcgta ggtggttaaa
       61 taagtcagat gtgaaatccc tgggctcaac ctaggaattg catttgatac tgtttagcta
      121 gagtatggta gagggaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaggcg acttcctgga ctaatactga cactgaggag cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005595
VERSION     KHUT01005595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          101 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggctggc
       61 caagtcgtgc gtgaaatctc tcggcttaac cgggagcggt cgtgcgagac tggccggctc
      121 gaggccggta gggggaagcg gaattccggg tgtagtggtg gaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccggtcctga cgctcaggag cgaaagcgtg
      241 ggtagcgatc cgg
//
LOCUS       KHUT01005596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005596
VERSION     KHUT01005596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           36 c           79 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcttat
       61 taagtcagtg tttaaagtct gcagcttaac tgtaggaatg gcattgatac tgataggctt
      121 gagtaaagtt gaggtaggcg gaattgaagg tgtagcggtg aaatgcatag atatcttcaa
      181 gaacaccgat tgcgaaggca gcttactaag ctttaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005597
VERSION     KHUT01005597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggtgctc
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggagtgctc
      121 gagtacggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005598
VERSION     KHUT01005598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccat
       61 taagtcagtg tttaaagtca atagcttaac tattggaatg gcaatgatac tgatgggctt
      121 gagtaaagtt gaggtgggcg gaattgatgg tgtagcggtg aaatgcatag atatcatcaa
      181 gaacaccgat tgcgaaggca gctcactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005599
VERSION     KHUT01005599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           94 g           45 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggtggttgca
       61 taagtcatcg ttgaaagctc ccggctaaac tgggagaggt gcggtgagac tgtgcggcta
      121 gagtatggga gagggatgca gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcatcctggc ctaaaactga cactgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005600
VERSION     KHUT01005600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgacgatctt
      121 gaggcactca ggggctgtcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaac ggtgaaaaca gacagctggg ggtgtcctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005601
VERSION     KHUT01005601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           68 c          100 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccccg
       61 ccagtcctgg gtgaaatctc ccggcccaac cgggaaagtg cccgggatac tgcggggctc
      121 gagcgaggca ggggcggggg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ccccgctggg cctctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005602
VERSION     KHUT01005602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggctttg
       61 taagtcagtg gtgaaatctc cccgctcaac ggggaaactg ccattgatac tgcagggctt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca ggttactacc aattgattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005603
VERSION     KHUT01005603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcaa
       61 caagtcagat gtgaaatccc ggggcttaac tccggacgtg catttgaaac tgttaggcta
      121 gagtgcagta gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005604
VERSION     KHUT01005604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctga
       61 caagtctggg gtgaaatacc acggctcaac cgtggaactg cctcagatac tgtcggcctc
      121 gagggaggta ggggcgtgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005605
VERSION     KHUT01005605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccga
       61 aaagtcaggg gtgaaatcct ccagcttaac tggagaactg cctttgatac tggcggtctg
      121 gagtaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcgtgctgga cctttactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005606             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005606
VERSION     KHUT01005606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           50 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcctat
       61 taagtcaggt gtgaaatctc cagggctcaa cccggaaact gcacttgata ctgttaggct
      121 ggagtgtcgg agaggtgatt ggaattcctg gtgtagcggt gacatgcatt gatatcagga
      181 ggaacaccca tggcgaaggc agatcactgg ccgacaactg acgctgaggc gcgaaagcgt
      241 gggtagcaaa caga
//
LOCUS       KHUT01005607             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005607
VERSION     KHUT01005607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggccttg
       61 taagtcagtc gtgaaatcgc tgggctcaac ccatgcaatt gcgattgata ctgcgagact
      121 tgaatggggt taaggcaggc ggaatgtggc atgtagcggt gaaatgcata gatatgccat
      181 agaacaccga ttgcgaaggc agcctgctgg gcctttattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005608
VERSION     KHUT01005608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcgt
       61 caagtcgggc gtgaaatctc tgggcttaac ccagaaaagt cgttcgatac tgttgagcta
      121 gagtgtggaa gaggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005609             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005609
VERSION     KHUT01005609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           81 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggcagag
       61 taagttactg gttgatgtca aactcaattt gacggaagct ggtaatactg ttttgctaga
      121 gggcaggaga ggttgataga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaaa aggcgaaggc agtcaactat cctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01005610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005610
VERSION     KHUT01005610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           97 g           49 t
ORIGIN      
        1 tacgggggga gcgagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggccatg
       61 caagtctgac gtgaaatgta ccggttcaac cggggcgctg tgtcagaaac tgcctggctt
      121 gaggtcgagg gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gccttctatc tcgattctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005611             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005611
VERSION     KHUT01005611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           52 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtcttg
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gctttgcata ctgcaggact
      121 agagggatgc agaggtttgt ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcaaactgg gcatcatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005612
VERSION     KHUT01005612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           96 g           39 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggccgc
       61 caagttcgag gtgaaatcct ccggctcaac cggagaactg cctcgaatac tggaggtctc
      121 gagggtggta ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccacatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005613
VERSION     KHUT01005613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           91 g           57 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggttcgt
       61 taagtttggt gttaaagacc agggctcaac cttgggaccg cactgaatac tggcggactg
      121 gagtgaagta gaggcaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgtaagcg gcttgctggg ctttaactga cgctgaggaa cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01005614             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005614
VERSION     KHUT01005614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           55 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggcattg
       61 taagttaggg gtgaaatccc atcggctcaa ccgtggaact gcctttaata ctgcaatgct
      121 tgagggaggt aggggaatgt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacattctgg atctcttctg acgctgaggc acgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01005615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005615
VERSION     KHUT01005615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggatgtt
       61 taagtccggt gtgaaagatc agggctcaac cctgagagtg catcggaaac tgggcatctt
      121 gaggacagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005616
VERSION     KHUT01005616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggatat
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tgatgtcctc
      121 gaggccgaga gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005617
VERSION     KHUT01005617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           79 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggctttg
       61 taagtcagtg gtgaaatgcc ggagcttaac tccggaactg ccattgatac tgcttagctt
      121 gaatcacgtc gaggtggatg gaatattaca tgtagcggtg aaatgcttag atatgtaata
      181 gaacaccgat tgcgaaggca gttcactagg cgtgtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005618
VERSION     KHUT01005618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcgggttcg
       61 tatgtcaatt gtgaaagccc cgggctcaac ctgggaattg cagttgaaac tgcgaatctt
      121 gaatacctga gagggtggga gaattcctgg tggagtagtg aaatacgtag agatcaggag
      181 gaataccgga ggcgaaggcg cctgcctggc agtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005619             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005619
VERSION     KHUT01005619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttttg
       61 aaagtcgggt gtgaaacctc caggctcaac ctggagacgc cactcgatac ttcaatgact
      121 tgagttcggt aggggtaagc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga cggcgaaggc agcttactgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01005620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005620
VERSION     KHUT01005620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacggaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggccgat
       61 caagtattga gtgaaagccc tgggcttaac ctgggaactg cttgatatac tgattggctt
      121 gagtcagaca ggggtgcctg gaacaatagg tggagcggtg aaatgcgtag atatctattg
      181 gaacgcctga ggcgaaagcg gggcactggg tctgcactga cgctgagacg cgaaagcgta
      241 gggagcgaac ggg
//
LOCUS       KHUT01005621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005621
VERSION     KHUT01005621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggataag
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg ccttggatac tgcttgtctc
      121 gagggagata ggggccgttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aacggctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005622             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005622
VERSION     KHUT01005622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           85 g           52 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgc
       61 taagtgagaa gtgaaatctc cagggctcaa cccggaaact gcttttcata ctggcagact
      121 tgaggaatgc agaggcaact agaattcccg gtgtagcggt gacatgcatt gatatcggga
      181 ggaataccca tggcgaaggc aggttgctgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005623             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005623
VERSION     KHUT01005623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           84 g           58 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agagtgcgta ggtggtttaa
       61 taagttattg gtgggaaatc ggggctcaac cccgtggaag tcggtaatac tgttagacta
      121 gaggaccgga ggggttaacg gaatggttca tgtaggggta aaatccatag atatgaatta
      181 gaacaccaat cggcgaaggc agttaactat tcggttcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01005624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005624
VERSION     KHUT01005624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           40 c           92 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttg
       61 taagttatgt gtgaaattcc tgggcttaac ctgggcgggt cagataatac tgcaagactt
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005625
VERSION     KHUT01005625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           87 g           50 t
ORIGIN      
        1 tacgtgagtg gcgagcgtta ttcggaatta ctgggcgtaa agggtccgca gggggcttgg
       61 taagtgcact gttaaatctg tcggctcaac cgataggccg cagtgcatac tgctaagcta
      121 gaggtatgca gaggtgagtg gaattcccgg agtaggggta atatccgtag ataccgggag
      181 gaacaccaat agcgaaggca gctcactggg cattatctga ccctcaggga cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005626
VERSION     KHUT01005626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 gacgaactgt gcaaacgtta ttcggaatca ctgggcttga agagtgcgta ggcggtttcg
       61 taagtagggt gtgaaagccc ccagctcaac tggggaactg cgtcctaaac tgcgaggctg
      121 gagtgaggta ggggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcacactgga ccttgtctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005627
VERSION     KHUT01005627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgtgca ggcggttttt
       61 taagtctgtg gtgaaagacc ggggctcaac cccggagtgt ccatggagac tggaagactg
      121 gagtgtcgga gaggagtgcg gaatccctgg tgtagcggtg gaatgcgtag agatcagggg
      181 gaacaccgat ggcgaaggca gcactctggc cgaatactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005628
VERSION     KHUT01005628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggttgt
       61 taagtcagtg gtgaaagatt cgagcttaac tcggaaactg ccattgatac tgatgacctt
      121 gaatacagtt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttgctaaa ctgttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005629             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005629
VERSION     KHUT01005629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c          102 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agagcgcgta ggcggcgcaa
       61 tacgacaggt gtgaaagccc ccggctcaac cggggagggt cgcctgtaac ggttgtgctc
      121 tgagtgcagg agagggatgt ggaactccgg gagtagcggt gaaatgcgta gagacccgga
      181 ggaacaccag tggcgaaggc ggcatcctgg tctgcgactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005630             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005630
VERSION     KHUT01005630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c          102 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgc
       61 cgcgtcggct gctgaaatcc cccggctcaa ccggggaagg ggcaccgaaa cgggcggcct
      121 ggaggggagt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg gctcctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01005631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005631
VERSION     KHUT01005631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttcat
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cccctgatac tgatgatctc
      121 gagggaggta ggggcctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgggctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005632
VERSION     KHUT01005632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttgg
       61 taagtcagac gtgaaagccc tcggcttaac cgaggaagtg catctgatac tgcctgactt
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005633
VERSION     KHUT01005633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcgcgta ggccgttcgc
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaattg catttgatac tggcgagctt
      121 gaggatgaga tgggtgggcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcccactgga ccatcactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005634
VERSION     KHUT01005634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctga
       61 taagtcggtc gtgaaagccc ttggcttaac caaggaactg cgatcgaaac tgccggacta
      121 gagtatcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005635
VERSION     KHUT01005635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgc
       61 taagttggac gtgaaaactc tgggcttaac ccagagcctg catccaatac tgacaagcta
      121 gagttttgga gggggaagcg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga caaaaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005636             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005636
VERSION     KHUT01005636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           83 g           53 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtgtctcgt
       61 taagtctgag gtaaaagcac tggagctcaa ctccgtgtat gtcttggaaa ctggcgagat
      121 tgagtcattg agaggtatct ggaatgtcgt gtgtaggggt aaaatccgta gatccacgat
      181 ggaacgccaa aagcgaaggc aggatactgg caatgtactg acactcagag acgaaagcgt
      241 ggggagcaaa gggg
//
LOCUS       KHUT01005637             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005637
VERSION     KHUT01005637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           76 g           56 t
ORIGIN      
        1 tcccagcggt ccaagtggca gccgcgttta ttgggtctaa aacatccgta gctcgcatcg
       61 taagtttcat gtgaaatctg gtctctcaag ggccaggcgt gcatgaaata ctgcgttgct
      121 agagaccggg agacgtaaga agtacagtta gggtagcggt aaaatgcgtt gatccttact
      181 agactaacga tagcgaaggc atcttacgag aacggatctg acagtgaggg atgaaggcta
      241 gggtcgcaaa acgg
//
LOCUS       KHUT01005638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005638
VERSION     KHUT01005638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggattca ctgggcataa agcgcacgta ggcggctggg
       61 tttgttcgat gtgaaagccc ccggctcaac cggggaattg catcgaatac ggcccggctc
      121 gaggatggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005639
VERSION     KHUT01005639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           88 g           53 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggtttg
       61 taagtcggat gtgaaatccc tgggcttaac ctaggaatgg cacccgaaac tataaatctt
      121 gaatacctaa gaggttggcg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaatatcgga ggcgaaggcg gccaactggt ggtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005640
VERSION     KHUT01005640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcaac
       61 caagtccgag gtgaaatcct ccagcttaac tggagaagtg ccccggatac tggtggcctc
      121 gaggagggta ggggcatctg gaactagcgg tggagcggtg aaatgcgttg atatcgctag
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005641
VERSION     KHUT01005641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacgct
       61 taagtcgggg gtgaaatccc ggggctcaac cccggaactg cctccgatac tgggtgtctg
      121 gaggtcggga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005642
VERSION     KHUT01005642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c          101 g           49 t
ORIGIN      
        1 tacggggggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgtgta ggcgggttgt
       61 taagtccgct gtgaaagccc ggtgctcaac gccggagggt cggtggatac tggcagtctt
      121 gaagtggcta gggggaaatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtttcctgga gctaacttga cgctgagacg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005643
VERSION     KHUT01005643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaactg ccattgatac tatctgtctt
      121 gaatattgtg gaggtttgcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gcaaactacg catatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005644
VERSION     KHUT01005644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          105 g           37 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggcggtctgt
       61 tgcgtaggcg gtgaaagccc caggctcacc ttggggaggt ccgtctaaac gagcagactg
      121 gagggcggta ggggctggcg gaacgcccgg agtagcggtg aaatgcgttg agatcgggca
      181 gaacaccagt ggcgaaggcg gccggctggg ccgttcctga cgctgaggcg cgaaagccag
      241 gggagcgaac gga
//
LOCUS       KHUT01005645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005645
VERSION     KHUT01005645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggcttga
       61 taagttagag gtgaaagccc ggggctcaac tccggaatag cctttaagac tgtctcgctt
      121 gaacgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgttga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005646
VERSION     KHUT01005646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           88 g           50 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggttatc
       61 caagtcaggt gtgaaagcct tgagctcaac tcaagaaatg cacttgatac tgggtgacta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggttactga cgctaagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005647
VERSION     KHUT01005647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           81 g           56 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagtacgta ggcggttatg
       61 caagacaatg gttaaatctc cgagctcaac tcggaacgtg ccgttgtgac tacataactt
      121 gtcgatagca ggggcaagca gaattgtcgg tgtaggagtg aaatccgttg agatcgacaa
      181 gaataccaat ggcgaaggca gcttgctagg ctatttgaga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005648
VERSION     KHUT01005648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcga
       61 taagttcgct ctgaaagtcc aaggctcaac cttgggatgc gggtgaatac tgtcgggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005649
VERSION     KHUT01005649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacatt
       61 taagttggag gtgaaagcct tgggctcaac ccaagaattg cctttgatac tggatgtctc
      121 gagaccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005650
VERSION     KHUT01005650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           83 g           54 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgaa gctaaatctc taggctcaac ctagaaacta tttcgcatac tggcgagctg
      121 gagtgatgaa gaggtttgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcaaactggt catcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005651
VERSION     KHUT01005651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcctta
       61 gaagtcagat gtgaaagccc cgggctcaac ctgggaatgg catttgatac tctgaggctg
      121 gagtacggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005652
VERSION     KHUT01005652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggtggcttga
       61 taagttcggc gtgaaatttc ccggcttaac cgggggatgt cgtcggagac tgtcgagcta
      121 gagggtgata gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg tcatacctga cactaaaacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005653
VERSION     KHUT01005653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           79 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcccat
       61 taagtcagag gtgaaagacg gtagctcaac tatcgcagtg cctttgatac tgatgggctt
      121 gaatgaacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactatg gttttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005654
VERSION     KHUT01005654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           79 g           55 t
ORIGIN      
        1 tacgaaggac ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactgt
       61 taagtcagtg gtgaaagctc cgagcctaac tcggaaactg ccattgatac tgacggtctt
      121 gagtacattt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag agatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005655
VERSION     KHUT01005655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           84 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggctgcatgg
       61 taagtcagat gtgaaatccg aaggctcaac ctttgaatag catccgatac tgccatgctt
      121 gagggctgta ggggggcttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacactcgt agcgaaagcg aaaccctggg cagcttctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005656             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005656
VERSION     KHUT01005656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           51 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtaga gtgaaatctc cagggctcaa cccggaaact gctctgcata ctggcagact
      121 tgagggactg agaggtgaat ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccca tggcgaaggc agttcactgg cagtcatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01005657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005657
VERSION     KHUT01005657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           95 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctttc
       61 taagtctatg gtgaaatctc ctggcttaac taggaggggt ctgtagatac tggaaggctt
      121 gagtaccgca gaggaagatg gaattcccgg tggagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgtaggcg gtcttctggg cggttactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005658
VERSION     KHUT01005658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          110 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggtcgg
       61 caagtcggtc gtgaaagccc cgggctcaac ccgggagggt cggccgagac tgccggtctt
      121 gagggaggca gaggagtctg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gggctctggg cctgacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005659
VERSION     KHUT01005659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggacgcg
       61 caagtcaggg gtgaaatccc ggggctcaac ctcggaattg cctttgagac tgcgtatctt
      121 gaggacggga gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctgga ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005660
VERSION     KHUT01005660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           79 g           56 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtccagg gttaaaaatt gaagcttaac ttcaaaggtg ccctagaaac ttttaaactt
      121 gaggatggta ggggtaaagg gaatttccag tggagcggtg aaatgcgtag atcttggaaa
      181 gaccaccaat ggcgaaggca ctttactggg ccatatctga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01005661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005661
VERSION     KHUT01005661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           98 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggtggctcgt
       61 tgcgtcggga gtgaaaacct gcggcttaac cgcaggcctg cttccgatac gggcgagcta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cactgagaag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005662
VERSION     KHUT01005662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatca ttgggcttaa agcgtgtgta ggcggcggcg
       61 taagtgtcga gtgaaatgcc agtgctcaac gctggaacgg ctcggcatac tgcgtcgctt
      121 gaggtcggta gaggcgcctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcctga ggcgaaagcg gggcgctggg ccgattctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005663
VERSION     KHUT01005663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggt
       61 caagtccggg gtgaaatcct gcagctcaac tgtagaagtg ccttggatac tggctggctc
      121 gagggagata ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acagactgga tctcttctaa cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005664
VERSION     KHUT01005664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttgaa
       61 caagtcatgt gttaaagccc ccggctcaac tgggggaggt catgtgagac tgtttgactt
      121 gagggcagca gaggtaggtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg acctactggg ctgcgactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005665
VERSION     KHUT01005665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg actagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtg
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cactcgatac tgctcgactt
      121 gaaggcggga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ccgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005666
VERSION     KHUT01005666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           95 g           40 t
ORIGIN      
        1 gacagaggcc ccgagcgcta atcggaatta ctgggcttaa agggtgcgta ggcggatccg
       61 taagcgtctt gtgaaagccc ccggctcaac cggggaacag cggggcgaac tgcggatctt
      121 gaggcaggta agggtcgctg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg agcgactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005667
VERSION     KHUT01005667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcatgca ggcggttttc
       61 taagtcggac gtgaaagctc ccggcccaac tgggagaggc cgttcgaaac tggaaaactt
      121 gaggatggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccattcctga cgctcagatg cgaaagctag
      241 gggagagaac ggg
//
LOCUS       KHUT01005668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005668
VERSION     KHUT01005668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcaag
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg ccttggatac tgcttgtcta
      121 gagggagata ggggttgctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agcaactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005669             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005669
VERSION     KHUT01005669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaagccc acggcttaac cgtgtggtct gcagtcgata cgggcaggct
      121 cgagttcggt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01005670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005670
VERSION     KHUT01005670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 tcagtcagat gtgaaagccc cgggctcaac ctgggaactg catttgatac tgcaaggctt
      121 gagttccgga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga cggacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005671
VERSION     KHUT01005671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtacga
       61 caagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgatac tgtcgcacta
      121 gagtcccgga gaggatggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005672
VERSION     KHUT01005672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 gacggagggt gcaagcgtta atcggaatta ttgggcgtaa agggcgcgta ggcggccttg
       61 caagtaggtt gtgaaatccc tgggcttaac ccaggaattg cagtctaaac tgcatggcta
      121 gagattagta gaggggggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacatcagt ggcgaaggcg accccctggg ctatatctga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005673
VERSION     KHUT01005673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           96 g           38 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggtaagg
       61 caagcgcgat gtgaaagctc ctggctcaac tgggagagtg cgtagcgaac tgcttaacta
      121 gaggccaggc gatgggggtg gaattcccgg tggagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgcaagcg gcccccaagc ctgaacctga cgctgaagcg cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005674
VERSION     KHUT01005674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          105 g           42 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggaggc
       61 caagtccgct gtgaaagccc ggggctcaac cccggcgggt cggtggagac tggtcttctt
      121 gaagatgcta gggggaggtg gaattatcgg tgtagcggtg aaatgcgtag agatcgatag
      181 gaacaccagt ggcgaaggcg gcctcctggg gcactcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005675
VERSION     KHUT01005675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtttgg
       61 caagtcttat gtgaaatctt cgggctcaac ccgaagcctg cataagaaac tgccgggctt
      121 gagtattgga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga caataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005676             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005676
VERSION     KHUT01005676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           80 g           59 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtataa
       61 taagttatca gtggcaaatc gaggctcaac ctcgtggaag ctggtaatac tgttatactt
      121 gagtgcagga gaggttaatg gaattccctg tggagcggta aaatgcgtta gatataggga
      181 ggaacatcaa taacggcgaa ggcaattaac tatcctgcta ctgacattga ggcacgaaag
      241 cgtggggagc aaaacgg
//
LOCUS       KHUT01005677             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005677
VERSION     KHUT01005677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           49 c           79 g           55 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggtta
       61 agaaagtcag atgtgaaatc tccgagctca actcggaatc tgtcatttga aacttcttga
      121 ctagaggggc aaagaggaag ctggaacgaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 tcggaacacc aatagcgtag gcaggcttct gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01005678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005678
VERSION     KHUT01005678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           92 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgtgta ggcggtctgt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cattggaaac tggcaaacta
      121 gagtacgtaa gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg acgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005679
VERSION     KHUT01005679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg cctttgatac tatctgtctt
      121 gaatatggtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacacccat tgcgaaggca gcttactaca cctatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005680
VERSION     KHUT01005680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           93 g           48 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgctcgta ggcggatcgg
       61 taagtctgtg aagaaagacc cgggctcaac tcggggaacg gcatagatac tgctgatctt
      121 gagacagtca gaggatgatg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg atcatctggg gctgttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005681
VERSION     KHUT01005681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           99 g           44 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggccagt
       61 tacgtccgct gtgaaatccc ggggctcaac cccgggcgtg cagtggatac gggctggctg
      121 gaggcaggca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005682
VERSION     KHUT01005682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggtggccgcg
       61 taagtcggat gtgaaagccc agggcttaac cctggaagtg catctgatac tgcatggctt
      121 gagtctcgga gagggtcgcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cgaagactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005683
VERSION     KHUT01005683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctta
       61 tttgtctggg gtgaaagcct tgagctcaac tcaagaagtg ccccggaaac ggtaagacta
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctgga caggcactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005684
VERSION     KHUT01005684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           83 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggattta ctgggcgtaa agcgtacgca gacggtgaga
       61 aaggtcgaat gtgaaagccc cgggctcaac ccgggaactg catccgaaac catctcgcta
      121 gagtatgaga gagggaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaatacctgc ggcgaaggcg gcttcctggc tcattactga cgttcaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005685             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005685
VERSION     KHUT01005685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           81 g           59 t
ORIGIN      
        1 tacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtgtga
       61 taagttatcg gtggtaaatc gaggcctaac ctcgtggaag ttggtaatac tgtcatactt
      121 gagggcagaa gaggttaacg gaactccctg tggagcggta aaatgtgtag atatagggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttcc tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01005686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005686
VERSION     KHUT01005686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctgt
       61 taagtctggt gtgaaatccc ctcgctcaac gagggaatgg cgccggaaac tggcagactc
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005687
VERSION     KHUT01005687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           94 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtga ctgggcgtaa agggcgcgta ggcggttaga
       61 ttagtctgat gtgaaagacc tgggcttaac ctgggatgtg cattggaaac ggtctagcta
      121 gagtatgaga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacactcgt ggcgaaggcg actttctggc tcaaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005688             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005688
VERSION     KHUT01005688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           40 c           79 g           58 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatca ttgggcgtaa agggtgcgta gatggcatga
       61 taagttatta gtgggaaatc gaggctcaac ctcgtggaag ctagtaatac tgttttgctt
      121 gagtacagaa gaggttaacg gaactccatg tggagcggta aaatgcgtag atatatggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01005689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005689
VERSION     KHUT01005689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaaagtg cctccgatac tgatcgcctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005690
VERSION     KHUT01005690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttca
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaatgg cgcttgttac tgtgaggcta
      121 gagtgtgtca gagggaggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gcctcctggg atagcactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005691
VERSION     KHUT01005691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 aacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggtccgta ggcggtgtag
       61 caagtcggaa gtgaaatctc tgggcttaac ccagaggctg cttccgaaac tgctgtgcta
      121 gagtgcgaga gaggcgagtg gaattgcggg tgtagcggtg aaatgcgtag atatccgcag
      181 gaacatccga ggcgaaagcg gctcgctgga tcgcaactga cgctgaggga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005692
VERSION     KHUT01005692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccatg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaacgg cacccgagac tgtgtggctc
      121 gagaccggaa gaggtgagtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctcactggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005693             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005693
VERSION     KHUT01005693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctctt
       61 taagtctggt gtttaatccc gaggctcaac ttcgggtcgc actggaaact ggggagcttg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005694             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005694
VERSION     KHUT01005694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          102 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaatgt
       61 taagcggggc cggaaagccc ccggctcaac cggggaaggg gccgcgaact ggcattcttg
      121 agggggagag gggagagcgg aattcctggt gtagcggtga aatgcgtaga gatcaggagg
      181 aacaccaatg gcgaaggcag ctctctggct tcaccctgac gctgaggcgc gaaagcgtgg
      241 ggagcgaacg gg
//
LOCUS       KHUT01005695             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005695
VERSION     KHUT01005695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           27 c           72 g           70 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agggcacgta ggtggtttgt
       61 tatgtgtatt attaaagttt aaagcataac tttaaattgt aatatagaca gctaaactag
      121 agttgggtag aggatagcag aatttctaat gtaaaggtga aatttgttaa aattagagag
      181 aatgcctaag gcgtaagcag ctatctgggc cttaactgac attgaggtgc gaaggcgtgg
      241 ggagcgaata gg
//
LOCUS       KHUT01005696             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005696
VERSION     KHUT01005696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           98 g           46 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtttca
       61 tgtgtccgct gtgaaacaac acggctcaac cgtgtagagc ggcggaaact gtgagacttg
      121 aggctgggag gggctggtgg aattccctgt gtagcggtga aatgcgtaga gatgggggag
      181 aacactcgtg gcgaaggcgg ccagctggac cagttctgac gctaagacgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005697             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005697
VERSION     KHUT01005697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtctat
       61 taagtctggt gtttaaggct gtggctcaac cacagttcgc actggaaact ggtagacttg
      121 agtgtaagag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggct tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01005698             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005698
VERSION     KHUT01005698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ttgggcgtaa agggtgcgta ggcggcccta
       61 tgggtcagag gttaaaggct acggctcaac cgtagtaagc ctttgaaact gtagggcttg
      121 agtgcaggag aggagagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctctctggac tgcaactgac gctgaggcac gaaagcgtgg
      241 gtagcgaaca gg
//
LOCUS       KHUT01005699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005699
VERSION     KHUT01005699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggttctt
       61 taagtccggg gtgaaagccg ggggctcaac ccccgaacgg cgccggaaac tggagagctt
      121 gagtgtcgga gaggggagtg gaattctcgg tgtagcggtg aaatgcgtag agatcgagag
      181 gaacacccgt ggcgaaggcg gctccctggc cgaacactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005700
VERSION     KHUT01005700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           97 g           41 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggaagtg
       61 caagtctcga gtgaaatccc gccgctcaac ggcggggctg cttgggatac tgcacctcta
      121 gggaccggta gaggctggta gaattgcagg tgtagcggtg gaatgcgtag agatctgcaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005701
VERSION     KHUT01005701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacgaagggt gcgaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtatgt
       61 taagttcgtt gttaaaggct tcagcctaac tggagttatg caatgaaaac tggcatgcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005702
VERSION     KHUT01005702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcgggtttg
       61 taagttgggg gtgaaatccc gaggctcaac ctcggaactg cctccaaaac tacaagtctg
      121 gacccaaaga gaggcgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg actcgctggc tttgtggtga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005703
VERSION     KHUT01005703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           98 g           45 t
ORIGIN      
        1 tacgaagggt gcaagcgtta gtcggaatta ctgggcgtaa agcgtgcgta ggcggttttt
       61 taagtctgcc gtgaaagccc cgggctcaac ctgggaatgg cggtggatac tggaaagctg
      121 gagtgcggta gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccctctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005704
VERSION     KHUT01005704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c          100 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgtgca ggcggttcgt
       61 taagtccggt gtgaaagctc ccggctcaac tgggagaggt cattggatac tggcggactc
      121 gagggaggca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctttcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005705
VERSION     KHUT01005705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           89 g           47 t
ORIGIN      
        1 gacgaggtcc acaaacgtta ttcggaatta ctgggcttaa agcgtgcgta ggcggcttag
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaattg cgcccgaaac tgctgagctt
      121 gagtgagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcactggg tctctactga cgctgaggta cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005706
VERSION     KHUT01005706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggtggtcttc
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccgaagac tggaaggctt
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccataactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005707
VERSION     KHUT01005707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           75 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggactga
       61 taagtcagcg gtgaaatctc tcggcttaac cgagaaactg ccgttgatac tgtcagtctt
      121 gagtacagat gatgtgggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtcactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005708
VERSION     KHUT01005708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgg
       61 caagttggat gtgaaagccc tgggctcaac ccgggaactg cattcaaaac tgccttgctt
      121 gaattcggta gaggttggtg gaattcccag tgtagaggtg aaattcgtag agattgggag
      181 gaacacccgt ggcgaaggcg gccaactgga ccgacattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005709
VERSION     KHUT01005709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 caagtcagat gtgaaatccc cgggcttaac ctgggaactg catttgagac tgcattgctc
      121 gagtatggga gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005710
VERSION     KHUT01005710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           99 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggatac tggggatctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005711
VERSION     KHUT01005711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtcttg
       61 taagttagat gtgaaatccc tgggcttaac ctaggaactg catctaagac tgtgagacta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005712
VERSION     KHUT01005712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccctc
       61 cacgtcggat gtgaaagctc acggctcaac cgtgagacgc cattcgatac gggaaggcta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005713
VERSION     KHUT01005713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           80 g           54 t
ORIGIN      
        1 tacgtagggg gcgagagttg tccggattta ctgggtgtaa agggtgctca ggcggattta
       61 caagtcagag gtgaaatacc ggggctcaac tccggcactg cctttgatac tgtaagccta
      121 gagattgaaa gagggtaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttacctggt tcaacactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005714
VERSION     KHUT01005714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcgtct
       61 caagtggatg gtgaaatccc cgggcttaac ctgggaactg ccgtccaaac tgagaggctt
      121 gagtccggga gaggtaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gcttactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005715
VERSION     KHUT01005715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaatctc caggctcaac ctggaaaagg cattggaaac tactcggctg
      121 gaggcttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005716
VERSION     KHUT01005716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           95 g           42 t
ORIGIN      
        1 gacagagggc tcaaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggctcgt
       61 taagtcggtt gtgaaatccc ccggctcaac cggggaacgg catccgatac tggcgggctc
      121 gaggacggga ggggtaagtg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactggc ccgtatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005717
VERSION     KHUT01005717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcaggt gtcaaatcct caggctcaac ctggggctcc acctgatact gctgtggcta
      121 gagtccggta ggggagcgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagc ggcgaaggcg gcgctctggg ccggaactga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005718
VERSION     KHUT01005718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           97 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgata
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagag catcgcaaac tgtattgctg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005719
VERSION     KHUT01005719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcaggg
       61 taagtcggat gtgaaatccc ggggctcaac ctcggaactg cattcgatac tgctttgctg
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005720
VERSION     KHUT01005720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctat
       61 caagtctagt gtgaaatccc ctcgctcaac gagggaactg cgccggaaac tgatagacta
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005721
VERSION     KHUT01005721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggagcgta ggcgggctat
       61 caagtctggg gtgaaatgcc gcggctcaac cgtggaactg cctcagatac tgacggcctc
      121 gagggagata ggggcatgcg gaacattggg tggagcggtg aaatgcgttg atatccaatg
      181 gaactccggt ggcgaaagcg gcgtgctgga tctcttctga cgctgaggct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005722
VERSION     KHUT01005722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          100 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcccga
       61 ttagtagggg gtgaaatctc gcggctcaac tgcggggctg cctcctaaac tgtcgggctc
      121 gagcagagta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ctcttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005723
VERSION     KHUT01005723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           87 g           56 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatta ttgggcgtaa agggttcgta ggcgggattg
       61 taagtcaagt gtgaaatccc cgagcttaac tcgggacgtg catttgaaac tgtgattctt
      121 gagtcattga gagggtggtg gaattgttgg tgtaggagtg acatccgtag agatcaacag
      181 gaacatcaga ggcgaaagcg gccacctggc aatgtactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005724
VERSION     KHUT01005724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           84 g           55 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtttgg
       61 taagtttgtt gtgaaatctc cgggcttaac ccggaaaatg caatgaaaac tgcctgactt
      121 gaggattgta ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caaatcctga cgctgatgca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005725
VERSION     KHUT01005725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggggtcg
       61 caagtcagat gtgaaatccc tgggctcaac ccaggaactg catctgaaac tgcgatcctt
      121 gagtactgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005726
VERSION     KHUT01005726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           92 g           41 t
ORIGIN      
        1 gacagaggcc ccgagcgtta atcggaatta ctgggcttaa agggtgcgta ggctgatccg
       61 aaagtgctcc gtgaaatccc ccggctcaac cggggaacag cgaggcatac tgcggatctt
      121 gaggcaggta ggcgccgagg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg ctcggctggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005727
VERSION     KHUT01005727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 tacgtagggt ccaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 taagtcagct gtgaaagccc cgggctcaac ctgggaattg cggttgaaac tggcgaacta
      121 gaatctggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005728
VERSION     KHUT01005728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           90 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccaag
       61 caggtcggat gtgaaatccc tcggctcaac caaggaagtg cattcgaaac cgcatggctc
      121 gagtcccgga gaggaaggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaagcg gccttctgga cgaggactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005729
VERSION     KHUT01005729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           78 g           61 t
ORIGIN      
        1 tacgaaggtc ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggtttt
       61 taagtcagtg gtgaaagcct tcagcttaac tgaagaatgg ccattgatac tgaagatctt
      121 gagtatgatt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005730
VERSION     KHUT01005730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggtggctaag
       61 tcagtcgatt gtgaaagccc tgggcttaac ctgggaattg cagtcgatac tacttggctc
      121 gagtatggga gagggcagtg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacatcagt ggcgaaggcg gctgcctggc ccaatactga cactcaggtg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005731
VERSION     KHUT01005731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcatg
       61 taagtggaat gtgaaatccc tcggcttacc cgaggacctg catcccaaac tgcatgactt
      121 gagtacggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccgtttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005732
VERSION     KHUT01005732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           83 g           50 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcta
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaactg cgcccaatac tgtagtgctt
      121 gaggaagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacccgt ggcgaaagcg gctcactgga tcttatctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005733
VERSION     KHUT01005733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           90 g           53 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggcgtaa agcgttcgta ggcggtgcag
       61 taagttaggt ttgaaagccc ggggctcaac tccggacccg gatctaaaac ttctgtgctt
      121 gagcatatca gggggcagtg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actgcctggg gtattgctga cgctgaggaa cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005734
VERSION     KHUT01005734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           93 g           42 t
ORIGIN      
        1 gacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcgagca ggcggttcgg
       61 taagtctgtc gtgaaagccc cgggctcaac ccggggaggt cgatggatac tgccggactc
      121 gaatacggga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgaa ggcgaaggca ggtgactgga ccgttattga cgctgagtcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005735
VERSION     KHUT01005735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           48 c           82 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggagtg
       61 tcagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tacacatcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgatgcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01005736             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005736
VERSION     KHUT01005736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           81 g           58 t
ORIGIN      
        1 aacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtggaa
       61 taagttattg gttgaagtcg agctcaactt gatggaggct agtaatactg ttctactgga
      121 gggcagaaga ggttagcgga actctcacga gtaggggtga aatccaatga tctgtggagg
      181 accaccaaaa aaggcgaagg cagctaacta ttctgttcct gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01005737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005737
VERSION     KHUT01005737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           46 c           98 g           54 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtctgtc gtgaaagacc agggcttaac tctggttttg cggtggatac gggcaggctt
      121 gaggcaagta ggggagaacg gaattccatg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gttctctggg cttgttctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01005738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005738
VERSION     KHUT01005738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           83 g           57 t
ORIGIN      
        1 gacgaggggt gcaaacgtta ttcggaatga ttgggtgtaa agggtgcgta ggcggtatgt
       61 taagtcaatc gttaaatttt tcagcttaac tgaaagtcag cggtagaaac tggcgtactt
      121 gaggatggaa gagagaagta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccaat ggcgaaggca gcttcttggt ccatatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005739
VERSION     KHUT01005739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacatactgt gcgaacgtta ttcggattca ctgggcttaa agggtgtgta ggcggcttgt
       61 taagtagggt gtgaaagccc ctggctcaac cagggaactg cactctatac tggcgagcta
      121 gagtgagata ggggtgtgtg gaactgccag tggagcggtg aaatgtgttg atattggcag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005740
VERSION     KHUT01005740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           81 g           65 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcttt
       61 taagtcagtg gtgaaatctc cgggctcaac ctggaaactg ccattgatac tattggtctt
      121 gaatattgtt gaggtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactggg caattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005741
VERSION     KHUT01005741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           81 g           56 t
ORIGIN      
        1 tacgaaggga gctagcgtta ctcggaatta ttgggcgtaa agcgtgcgta ggcggctttg
       61 taagtcaaaa gtgaaagcct ggggctcaac ctcagaattg cttttgatac tgcaaagcta
      121 gagtatcaga gaggatagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccaga ggcgaaagcg gttatctggc tgagtactga cgctgttgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005742
VERSION     KHUT01005742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          109 g           35 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccctg
       61 tgcgctgccg gtgaaagctc ccggcttacc tgggagaggt tcggcaggac ggcagggctg
      121 gagggtggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt agcgaaggcg gccgactgga ccacacctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005743
VERSION     KHUT01005743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatta ctgggcgtaa agggggcgca ggcggttcgc
       61 caagttgaaa gtggaagccc ggggctcaac cccggattgt ctttcaatac tagcgaactt
      121 gagtttggga gaggaaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccgat ggcgaaagca gctttctggc ccgatactga cgctcatgcc cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005744
VERSION     KHUT01005744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c           99 g           48 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtctta
       61 taagtatggc gtgaaatctc cctgctcaac ggggagggtg cgttgtagac tgtgagactt
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgacc cgg
//
LOCUS       KHUT01005745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005745
VERSION     KHUT01005745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggttcct
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaactg cgcttgagac tgcgggactg
      121 gagttcggga gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005746
VERSION     KHUT01005746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           91 g           44 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttag
       61 caagttttct gtcaaagatc agggctcaac cctgggccgg cagagaaaac tactaggcta
      121 gagttcggta ggggcagagg gaattcctgg tggagcggtg aaatgcgtag agatcaggag
      181 gaacaccgaa ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagcgag
      241 gggagcaaat ggg
//
LOCUS       KHUT01005747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005747
VERSION     KHUT01005747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggcggtgact
       61 caagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgaaac taggtcgcta
      121 gaatgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005748
VERSION     KHUT01005748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           75 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcctg
       61 taagtcagtg gtgaaatctt tcggctcaac cggaaaattg ccattgatac tgcaggcctt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca ggttactacc aatacattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005749
VERSION     KHUT01005749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          103 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggacctg
       61 gacgtgccgt gtgacaaccc atcgctcacc gatggagggc cacggtacac gacaggtctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggt cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01005750             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005750
VERSION     KHUT01005750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           50 c          101 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa aaggcttgta ggtggccatg
       61 cgaagtcgga tgtgaaatct cccggctcaa ctgggagggg tcattcgata ctggcaggct
      121 cgaggccggt agggggtagc ggaattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggctacctgg accggttctg acactgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01005751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005751
VERSION     KHUT01005751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagtcgaac gtgaaatccc cgggcttaac ccgggaactg cgtccgatac tgggaggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005752
VERSION     KHUT01005752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           95 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccga
       61 taagtctgcc gtgaaagcca agggctcaac ccttggatgc cggtggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctcgctggg acggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005753
VERSION     KHUT01005753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          100 g           42 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ttgggcgtaa agggcgcgta ggcggcgcgg
       61 ttggtcaggc gtgaaatccc cgggctcaac ccagggactg cgcttgatac ggccgcgctg
      121 gaggatggaa gagggccgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcggcctggt ccatttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005754
VERSION     KHUT01005754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          102 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcctgt
       61 cgcgtcggtc gtgaaagccc ggggctcaac cccgggtctg cggtcgatac gggcaggctg
      121 gagttcggca ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005755
VERSION     KHUT01005755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           99 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 cgagtcgggg gtgaaatccc acagcttaac tgtggaagtg ccttcgatac tggcgggctc
      121 gagggaggta ggggtccgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcggactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005756
VERSION     KHUT01005756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttctg
       61 caagtcagat gtgaaatccc cgggctcaac ctgggaactg cgtttgaaac tacagagcta
      121 gagtatggga gagggaggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccaat ggcgaaggca gcctcctggc ctaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005757
VERSION     KHUT01005757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           90 g           44 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggccagg
       61 caagttcgat gtgaaagccc cgggcttaac ccgggaatgg cattgaaaac tgcatggctg
      121 gaggatcgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005758
VERSION     KHUT01005758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggtcgg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccattgatac tatcgatctt
      121 gaatactgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca catatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005759
VERSION     KHUT01005759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggtttc
       61 taagtcgggg gtgaaagcct gtggctcaac cacagaatgg ccttcgatac tggcaacctt
      121 gagaccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccgggtctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005760
VERSION     KHUT01005760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           87 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatccg
       61 taagtacctt gtgaaatccc acagcttaac tgtggaactg cttggtatac tgcgggtctt
      121 gagccactca ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005761
VERSION     KHUT01005761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           96 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggttttc
       61 caagtttgtt gtgaaatctc ccggctcaac cgggagggtg cggcgaaaac taggagactg
      121 gagtgctgga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggtgtagaca gcttgctgga cagtgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005762
VERSION     KHUT01005762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          108 g           36 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgca ggcggccgtg
       61 taagtccgct gtgaaagacc ggggctcaac cccggggtgt cagcggggac tgcacggctg
      121 gagtcccgga gagggaggcg gaatacctgg tgtagcggtg gaatgcgtag agatcaggtg
      181 gaacaccgat ggcgaaggca gcctcctgga cgggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005763
VERSION     KHUT01005763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           64 c          106 g           31 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcgggccgt
       61 gacgtgccgt gtgacagctg ccggcttaac cggcagaggc cagggtagac gcacgacctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagccaac cgg
//
LOCUS       KHUT01005764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005764
VERSION     KHUT01005764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctaca
       61 caagtcagag gtgaaagccc ggggctcaac tccggaactg cctttgaaac tgggtggcta
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005765
VERSION     KHUT01005765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtcgtt
       61 caagtcggat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgatcgactt
      121 gaaggcagga gagagaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcttcttggc ctgttcttga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005766
VERSION     KHUT01005766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ttgggcatac tggcggcctt
      121 gaggcacgca ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gctcgctggg cgtgtgctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005767
VERSION     KHUT01005767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           95 g           48 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcacgg
       61 ttagtcaggt gtgaaatctc cacgctcaac gtggaggggt cacctgatac tgccgagctt
      121 gagtccggta ggggagtgtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacacccgt agcgaaggcg gcactctggg ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005768
VERSION     KHUT01005768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctgt
       61 caagtcatct gtgaaatctc cgggcttaac tcggaacggt cagatgatac tgtcaggcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005769
VERSION     KHUT01005769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          108 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 taaggctgtg gtgaaagccc ggggctcaac cccgggtctg ccgtggcgac tgctgggctg
      121 gagcactgta gaggcgggta gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaataccggt ggcgaaggcg gcccgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005770
VERSION     KHUT01005770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggctgga
       61 aaagtctgat gtgaaatccc ggggctcaac cccggaactg cactggatac ttttcagcta
      121 gagatctgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaaggccagt ggcgaaggcg agtttctgga cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005771
VERSION     KHUT01005771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggtgagc
       61 ctagtctgcc gtgaaatctc ttcgcttaac gaagaggggt cggtggaaac tagcttacta
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005772
VERSION     KHUT01005772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggtctag
       61 ccagtctgga gtgacagttc ccggctcacc tgggaaaggt cttcggaaac tgctagactt
      121 gagggactca gagggagacg gaattccagg tggagcggtg aaatgcgtag agatctggag
      181 gaacaccaaa ggcgaaggca gtctcctggg agttacctga cgctcaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005773
VERSION     KHUT01005773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggcccat
       61 caagtcagat gtgaaataac caggcttaac ctggtggggt catttgaaac tggcgggcta
      121 gagtccagca gaggatagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctggg ctggaactga cgctgaggcg cgaaggctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005774
VERSION     KHUT01005774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          101 g           41 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtggga
       61 caagtgggtg gtgaaacctc ccagcttaac tgggagccgg ccatccaaac tgtcccgctg
      121 gagtgtggga ggggaaagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gctttctgga ccactactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01005775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005775
VERSION     KHUT01005775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtatg
       61 taagtcagtg gtgaaatctt ggagcttaac tccgaaactg ccattgatac tatgtatctt
      121 gaatatcgtt gaggtttgcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcaggctaaa cgaacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005776
VERSION     KHUT01005776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggttatt
       61 caagtctgct gttaaagcgc gaggcttaac ttcgtacagg cagtggaaac tgaatgacta
      121 gagtatggta ggggtagcgg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagc ggcgaaagcg cgctactggg ccataactga cactcatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01005777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005777
VERSION     KHUT01005777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           90 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 aaagtcaggg gtgaaatccc ccagcttaac tggggaactg cctttgatac tgaatgtctc
      121 gagtaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaggcg gcatgctgga ccttcactga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005778
VERSION     KHUT01005778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           92 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggctctg
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgtcgaaaac tgcagggcta
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctttctgga ccataactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005779
VERSION     KHUT01005779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           90 g           53 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taagtgtgaa gtgaaagctc cgggctcaac tcggaaattg cttcgcatac tggctagctt
      121 gagggatgca gaggtttgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcaaactggg cattacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005780
VERSION     KHUT01005780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcgacg
       61 acagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgcttgatac atcgttgcta
      121 gaggacggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgttactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005781
VERSION     KHUT01005781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctacg
       61 gcagtcgggt gtgaaagccc ggggctcaac cccggaactg catccgatac tctgtggcta
      121 gagaccggaa gaggtgggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005782
VERSION     KHUT01005782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 taagtctgtt gtgaaagtcc agggctcaac cctggaaagc cgatggatac tgccgggcta
      121 gagttcggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gctcgctggg acgacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005783
VERSION     KHUT01005783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcgggagcg
       61 aaagtccggt gtgaaagccc ccagctcaac tggggaaggt cgctggaaac tgcgcttctt
      121 gagggcggca gagggaagtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctggg ccgcgcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005784
VERSION     KHUT01005784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 aaagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaatac ttccagactt
      121 gaggatggta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg acctcctgga ccatttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005785
VERSION     KHUT01005785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           96 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtgtcc
       61 taagtctgtt gtgaaatctc ccggcttaac cgggagggtg cggcggaaac tgggatgctg
      121 gagtgcggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttactgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005786
VERSION     KHUT01005786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           95 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttccg
       61 taagtttgtt gtgaaatctc ccggcttaac tgggagggtg cggcgaaaac tgcggggctt
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005787
VERSION     KHUT01005787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtctgtg gtgaaatcct tcggctcaac cggagaatag ctggagatac tggccgtctc
      121 gaggggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005788
VERSION     KHUT01005788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          101 g           42 t
ORIGIN      
        1 tacggagggg gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcggtcgtg
       61 cgcgtcggag gtgaaatccc cgggcttaac ccgggagctg cctccgatac ggcgcggctt
      121 gagtccggga gaggggagca gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaataccggt ggcgaaggcg gctccctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005789
VERSION     KHUT01005789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtcttt
       61 caagttcgga gtgacagttg tcggcttcac cgagaaagtg ctttgaagac tgagagactt
      121 gagggctcaa gagggacacg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa tgcgaaggca gtgtcctggg gagcacctga cgctcatgtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005790
VERSION     KHUT01005790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           86 g           49 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgttgggt gtgaaagccc ggagcttaac tccggaattg cgctcaaaac tacacggcta
      121 gagtattgga gagggtaacg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccaga ggcgaaggcg gttacctgga caattactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01005791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005791
VERSION     KHUT01005791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           77 g           61 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggtcttt
       61 taagtcagtg gtgaaatcct gccgcttaac ggtagaattg ccattgatac tgaaagactt
      121 gagtccagtt gaggtaagcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gcttactaaa ctggcactga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005792
VERSION     KHUT01005792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtctggt gtgaaatccc ggggctcaac ctcggaactg cactggatac tgccctgcta
      121 gagatctgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005793
VERSION     KHUT01005793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c           98 g           50 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgatc gtgaaaactt ggggcttaac tccaagcttg cggtcgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005794
VERSION     KHUT01005794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           89 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcactt
       61 caagtctgtc gtcaaagacc ggggcttaac cctggaaagg cgatggaaac tgaaaggcta
      121 gagagaggta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga cctcatctga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01005795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005795
VERSION     KHUT01005795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           98 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ttgggcgtaa agggcgcgta ggcggccgga
       61 tttgtcaggc gtgaaaatcc tgggctcaac ccagggactg cgtttgaaac ggtccggctg
      121 gaggacggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccgttactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005796
VERSION     KHUT01005796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttatg
       61 taggtgagtt gtgaaatccc tgggcttaac ctaggaactg caacttaaac gatatagctg
      121 gagtacggta gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005797
VERSION     KHUT01005797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           88 g           53 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttacc
       61 taagtctggt gttaaagccc ggagctcaac tccggcatgg cattggaaac tgggtggcta
      121 gagtatggta gaggttacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg cgtgactgga ccataactga cgctgaggaa cgaaagccag
      241 ggtagcaaaa agg
//
LOCUS       KHUT01005798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005798
VERSION     KHUT01005798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 tgcgtccatc gtgaaaactc agggcttaac tctgagcttg cggtggatac gggcgggcta
      121 gagttcggta ggggagtctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggactctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005799
VERSION     KHUT01005799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtctaa agggtgcgta ggcggtttgt
       61 taagtcagtg gtgaaagacg gcagctcaac tgtcgcagtg ccattgatac tggcgaactt
      121 gaatgtcgat gaggccggcg gaattggtgg tgtagcggtg aaatgcatag ataccaccag
      181 gaacaccgat tgcgaaggca gctggctaat cgaatattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005800
VERSION     KHUT01005800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           87 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcgggacat
       61 taagtccgag gtgaaagacg gttgctcaac aatcgcagtg ccttggatac tggtgttctt
      121 gggtgaagtt gaggtgggcg gaattcaagg tgtagcggtg aaatgcatag ataccttgaa
      181 gaactccgat tgcgaaggca gcccactgga cttctaccga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005801
VERSION     KHUT01005801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 gacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacctg
       61 taagtacttt gtgaaatccc ccggctcaac cggggaactg cttggtatac tgcgggtctt
      121 gagccactta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005802
VERSION     KHUT01005802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggtggtcatg
       61 taagtccgct gtgaaagtca aaggctcaac ctttgaaagc cggtggatac tgcatgacta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005803
VERSION     KHUT01005803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           90 g           51 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggttggt
       61 caagtcactt gtgaaatctc tgagcttaac tcagaacggc caagtgatac tgattaacta
      121 gagtgcagaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005804
VERSION     KHUT01005804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           39 c           79 g           56 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtccaat gttaaaaatt gaagcttaac ttcaaaggtg ccttggaaac ttttaaactg
      121 gaggatggta ggggtagagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaccaccaat ggcgaaggca ctctactggg ccattactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01005805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005805
VERSION     KHUT01005805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           78 g           59 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggcggattgg
       61 taagttagag gtgaaactcc gaggctcaac ttcggaactg cctttaatac tgccaatctt
      121 gagattagga gaggttgatg gaatgtaagg tgtagcagtg aaatgtgtag atatcttaca
      181 gaacaccaat ggcgaaggca gtcaactggc ctatatctga cgctaaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005806
VERSION     KHUT01005806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           97 g           40 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggtggg
       61 caagtcggaa gtgaaatctc gccgctcaac gacgaggctg cttcggatac tacccatcta
      121 gggaccggta gaggctggta gaattgcagg tgtagcggtg gaatgcgtag agatctgcaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005807
VERSION     KHUT01005807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           77 g           59 t
ORIGIN      
        1 gacgaaggac ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcgggttga
       61 taagtcagtg gtgaaagccc aacgcttaac gttggaactg ccattgatac tgtcgatctt
      121 gaatgtgttt gatgtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaag ccactattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005808
VERSION     KHUT01005808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           96 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatta ttgggtgtaa agggtgtgta ggcggggcgg
       61 taagtcaggg gtgaaagtct acggctcaac cgtaggactg cctttgatac tgtcgtcctt
      121 gagtcccgga gaggccacta gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaataccaga agcgtaggcg ggtggctgga cggcgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005809
VERSION     KHUT01005809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaga
       61 taagttaggt gtgaaagtcc tgggcttaac ctggggattg cgcctaatac tgtgtaacta
      121 gagtgctgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaggcg actttctgga cagacactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005810
VERSION     KHUT01005810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacggaggat gcgagcgttg ttcggaatca ctgggcttaa agcgcatgta ggcggaatgg
       61 agggtgcttt gtgaaagccc ccggctcaac cggggaattg cttggcaaac cgccattctt
      121 gaggcaggta gaggcgactg gaacctttgg tggagaggtg aaattcgtag atatcaaagg
      181 gaacgccggt ggcgaaagcg ggtcgctggg cctgtcctga cgctgagatg cgaaagcgta
      241 gggagcgaac ggg
//
LOCUS       KHUT01005811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005811
VERSION     KHUT01005811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgca ggcgggcctg
       61 taagtcaggg gtgaaagcca tcggcttaac cgatgaattg cccttgatac tgcaggtctt
      121 gagtctcgga gaggttggcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga agtgtagacg gccaactgga cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005812
VERSION     KHUT01005812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           95 g           44 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggcggatcat
       61 taagtcactg gtgaaagcac cgggctaaac tcggtgaggt tcggtgagac tggtggtctg
      121 gagtatgtca gaggggtgcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcaccctggg acaaaactga cgctgagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005813
VERSION     KHUT01005813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           53 t
ORIGIN      
        1 tacggagacc tcgagcgtta ctcggattta ctgggcgtaa agggttcgta ggtggccaag
       61 tgtgtcgggt gtgaaatctt gcggcttaac cgtaaaactg cgcccgaaac tacttggcta
      121 gagtcttgga ggggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaac ggcgaaggca gcttactgga caagtactga cactgaggaa cgaaagtatg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01005814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005814
VERSION     KHUT01005814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttttg
       61 taagtcagtg gtgaaatact ctagcttaac tagaggggtg ccaatgatac tgcaagactt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactggg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005815
VERSION     KHUT01005815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           98 g           37 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggacaag
       61 caagtcacgt gtgaaagcgc ccggctcaac cgggcgagga cgggtgaaac tgcttgactg
      121 gaggccggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctgga ccgagcctga cgctcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005816
VERSION     KHUT01005816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtggtt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaattg cgatggatac tggatcacta
      121 gagtgtggta gagggatgcg gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaacatctgt ggcgaaggcg gcatcctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005817
VERSION     KHUT01005817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gtcggccatt
       61 taagtcgggt gtgaaatccc cgggctcaac ctgggaattg cattcgatac tggatggcta
      121 gagttcggca gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcttcctgga ccagaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005818
VERSION     KHUT01005818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgcgta ggcggtcttt
       61 caagtccggt gtgaaaactt gaggctcaac ctcaagcgtg catcggatac tgatggacta
      121 gagttcggta gagggaatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg gattcctggg ccgatactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005819
VERSION     KHUT01005819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           94 g           44 t
ORIGIN      
        1 aacgtagggt tcgagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggcggttagg
       61 gaagtcggac gtaaaagctc ctggcccaac tgggagaggc cgttcgaaac tccttaacta
      121 gaggctagta gaggcaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaagaccaga ggcgaaggcg gcttgctggg ctagacctga cgctgagacg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01005820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005820
VERSION     KHUT01005820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggatggt
       61 caagtcgggg gtgaaatccc ggggctcaac cccggaactg cctccgatac tggacgtctg
      121 gaggacggaa gaggcgagtg gaatgccgag tgtagaggtg aaattcgtag atattcggcg
      181 gaacacccgt ggcgaaggcg gctcgctggt ccgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005821
VERSION     KHUT01005821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           84 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtaagg
       61 taagtttgat gtgaaagctc cgagcctaac tcggaaattg cattgaaaac tgcctaacta
      121 gaggattgga ggggggactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caaatcctga cactgaggca cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01005822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005822
VERSION     KHUT01005822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcagc
       61 caagtcgggg gtgaaatctt tcggcccaac cggaaaaggg cctccgatac tggctgcctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005823
VERSION     KHUT01005823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           95 g           51 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agggagcgca ggtggttgcg
       61 caagtcggtg gtgcaagctc caggcttaac ctggagaggt ctgtcgatac tgcgtaactt
      121 gagggcggta gaggagcacg gaattcctgg tgtagtggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gtgctctggg ccgttcctga cactcatgct cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005824
VERSION     KHUT01005824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           97 g           44 t
ORIGIN      
        1 aacgtaggac acgagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggttttg
       61 taagtttgat gtgaaagcgc ccggcttaac cgggcgagga cattggagac tgcaagacta
      121 gaggcagata gaggggtgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcaccctggg tctgacctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005825
VERSION     KHUT01005825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtggcc
       61 caagtctggt gtgaaatctc ccggcttaac cgggagggtg cgccggaaac tgggttgctc
      121 gagtgccgga gaggcaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggtgtagacg gcctgctgga cggtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005826
VERSION     KHUT01005826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           89 g           57 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggattg
       61 caagtcagat gtgaaattct tgggcttaac tcaagagctg catctgaaac tgtgattctt
      121 gagtactgga gaggatagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctatctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005827
VERSION     KHUT01005827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           89 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcgctg
       61 taagtcaggg gtgaaaggtt ccggctcaac cggagaattg cctttgatac tgcagtgcta
      121 ggattgggtt gaggttggcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccgat tgcgaaggca gccaactgga ccttgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005828
VERSION     KHUT01005828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcccac
       61 caagtcggac gtgaaagccc tcggcttaac cgaggaagtg cattcgatac tagtgggctt
      121 gagttcggga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga tcgaaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005829
VERSION     KHUT01005829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacgaaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcatgta ggcggggtgc
       61 caggtacctt gtgaaatccc tcggctcaac tgaggaatag cttggtaaac cggtactctt
      121 gaggcaggta gaggcgggcg gaaccatggg tggagcggtg aaatgcgtag agatccatgg
      181 gaacaccggg ggcgaaagcg gcccgctggg cctgatctga cgctgagatg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005830
VERSION     KHUT01005830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           78 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtattt
       61 taagtcagtg gtgaaatgtt gcagcttaac tgtagaattg ccattgaaac tgaagtactt
      121 gagttatctt gaggtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactaag gataaactga cgctcaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01005831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005831
VERSION     KHUT01005831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgtgtgca ggcggaacga
       61 taagtggtgt atgaaatttc ccagcttaac tgggaggggt tatgccagac tgtcgttcta
      121 gagggtgaga gaggagcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgctctggc tcacccctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005832
VERSION     KHUT01005832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           81 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgagtgta ggcgggaggt
       61 taagtttgct gttaaatctc atagctcaac tatgaaatgc cggcgaatac tggccttctt
      121 gaattcggta ggggtaactg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacgccaaa ggcgaaagca ggttactggg ccgcaattga cgctgagact cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005833
VERSION     KHUT01005833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           77 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacctt
       61 taagtcagtg gtgaaagttt gcggctcaac cgtaaaattg ccattgaaac tgaaggtctt
      121 gagtgtaaat gaggtaggcg gaatgtgttg tgtagcggtg aaatgcttag atataacaca
      181 gaacaccaat tgcgaaggca gcttactagg atacaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005834
VERSION     KHUT01005834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggtccgc
       61 taagtctgat gtgaaatccc ccggctcaac cggggaactg cattggatac tggcgggcta
      121 gagagttgta ggggtggacg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccgga ggcgaaagcg gtccactggg caacttctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005835
VERSION     KHUT01005835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           96 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 atagttgggc gtgaaaggcc tgggctcaac ccgggaactg cgttcaatac tgtaaggcta
      121 gagtgtggga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005836
VERSION     KHUT01005836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           74 g           64 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggattgg
       61 taagtcagtg gtgaaatcct acagcttaac tgtagaattg ccgttgatac tgccagtctt
      121 gaatatagtt gaggtggatg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat cgcgtaggca gttcaccaaa ctattattga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01005837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005837
VERSION     KHUT01005837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggcacg
       61 caggtcagat gtgaaatccc acggctcaac cgtggaactg catttgaaac cgtgtgtctt
      121 gagcaaacta ggggtatgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccggt ggcgaaagcg gcatactggg gtttgactga cgctgaggca cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005838
VERSION     KHUT01005838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           76 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcttg
       61 aaagtcagtg gtgaaatctc cgtgcttaac atggaaactg ccattgatac tctgagtctt
      121 gaattttctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg ggaacattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005839
VERSION     KHUT01005839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           89 g           53 t
ORIGIN      
        1 gacggaggag gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggttgtgagt
       61 tcagttggat gtgaaagccc ggggcttaac ctcggatgtg catccaatac tggctcgctg
      121 gaggttggaa gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ccatacctga cactcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005840
VERSION     KHUT01005840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           94 g           41 t
ORIGIN      
        1 aacgtaggac gcaaacgtta tccggaatta ctgggcgtaa agagcgtgta ggcggtttgg
       61 taagtcaacc gtgaaagctc ccggctaaac tgggagaggt cggctgagac tgccagacta
      121 gagggtgtta gagggatgcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcatcctggg acaaacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005841
VERSION     KHUT01005841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           85 g           57 t
ORIGIN      
        1 tacgtggggt gcaagcgtta tccggattta ttgggcgtaa agagttcgta ggcgtcttgt
       61 taagttctgt ttgaaatccc gaggcttaac ctcggaactg gatggaatac tagcaagatc
      121 gagtctgtta gggggtactg gaactcatgg tgtagcagtg aaatgcgttg atatcatgag
      181 gaacaccaag ggcgaaggca ggtacctggg acacgactga cgctgaggaa cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01005842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005842
VERSION     KHUT01005842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           83 g           48 t
ORIGIN      
        1 tacagagact ccaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccagg
       61 tgtgtcagag gtgaaatact gcagcttaac tgtagaactg cctttgaaac tacctggcta
      121 gagtatcgga gagggaagcg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccaaa ggcgaaggca gcttcctgga cgattactga cgctcaggca cgaaagcatg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01005843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005843
VERSION     KHUT01005843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           87 g           51 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgca ggcggatcag
       61 taagttactc atgaaagctc ccggctaaac tgggagaggt tgagtgatac tgctggtcta
      121 gagtggatga gaggtaggta gaattccttg tgtagtggtg aaatgcgtag atataaggaa
      181 gaataccagt ggcgaaggcg acctactggc atccaactga cgctcagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005844
VERSION     KHUT01005844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtccgtt gtgaaagccc tgggctcaac ctgggaactg cagtggatac tggcaagcta
      121 gagtgcggta gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcgat ggcgaaggca gccctctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005845
VERSION     KHUT01005845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtctag
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cattcgaaac tactaggctt
      121 gagtactgaa gaggacgacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtcgtctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005846
VERSION     KHUT01005846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           93 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatg
       61 taggtccagg gtgaaatccc ccggctcaac cggggaactg ccttggaaac cccacgcctt
      121 gagggagaca ggggcacatg gaacttccag tggagcggtg aaatgcgtag agattggaag
      181 gaacgccggt ggcgaaagcg atgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005847
VERSION     KHUT01005847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatatt
       61 taagtgaggg gtgaaatccc agggctcaac cctggaactg cctctcatac tgggtatctc
      121 gagttcgaga gaggtgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005848
VERSION     KHUT01005848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           96 g           46 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggttacg
       61 taagtcgtgc gtgaaagcgc tctgcttaac agggcgagga cgggcgagac tgcgtgacta
      121 gagctagata gaggcaagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgaaggcg gcttgctggg tctaagctga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005849
VERSION     KHUT01005849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           84 g           51 t
ORIGIN      
        1 tacggagact gcaagcgtta ctcggattca ctgggcgtaa agggtgcgta ggcggctagg
       61 tgtgtcaggt gtgaaatctc ggggcttaac ctcgaaactg cgcctgaaac tacctagcta
      121 gagtattgga gaggtaagcg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacaccaat ggcgaaggca gcttactgga caataactga cgctgaggca cgaaagcatg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01005850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005850
VERSION     KHUT01005850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtttcg
       61 caagtcagag gtgaaatccc caggctcaac ctgggaattg cctttgttac tgcgagacta
      121 gaggccggga gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005851
VERSION     KHUT01005851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           93 g           49 t
ORIGIN      
        1 tacgtaggtg gcgaacgttg tccggattca ctgggcgtaa agcgcgcgca ggcggattgg
       61 taagtctgag gtgaaagctc ccggctcaac tgggagaagc ccttggatac tgccaatctt
      121 gagacactta gaggagagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg agtgttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01005852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005852
VERSION     KHUT01005852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcacgca ggcggcttat
       61 caagttgggg gtgaaatccc agggctcaac cctggaactg cctccaaaac tggtaagcta
      121 gagatcgaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgaatctga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005853
VERSION     KHUT01005853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           91 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcttt
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaatac tgggagactt
      121 gaggcaatga gaggggtgta gaattccagg tgtagtggtg gaatgcgtag atatctggag
      181 gaataccagt ggcgaaagcg gcaccctagc gttgttctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005854
VERSION     KHUT01005854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggagcgta ggcggcgtgt
       61 caagtcagtt gtgaaatctc cgagcttaac tcggaacggt caactgatac tgtcacgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgaggct cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005855
VERSION     KHUT01005855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           98 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtttgg
       61 taagtctgct gtgaaatctc ccggctcaac tgggaggggt cagcggatac tgccaggctt
      121 gaggcaggca gaggaaggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctggg cctgtcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005856
VERSION     KHUT01005856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggactgg
       61 aaggtctggt gtgaaagccc ggggctcaac cccggagggt caccggaaac cgacagtctt
      121 gaatctggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acatgattga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005857
VERSION     KHUT01005857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           83 g           50 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgca ggcggtcttg
       61 caagtcagaa gtgaaagccc ggggcttaac cccggaattg cttttgaaac tgcaagacta
      121 gaatatggga gaggttagca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgaaggcg gctaactgga ccattattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005858
VERSION     KHUT01005858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatgtt
       61 taagtcgggg gtgaaagccc ggggctcaac ctcggaattg ccttcgatac tggacatctt
      121 gagtacggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactggc ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005859
VERSION     KHUT01005859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggctat
       61 taagtcagtg gtgaaatctc cgagctcaac ttggaaactg ccattgatac tattagtctt
      121 gaattctgtt gaggtaggcg gaataagtca tgtagcggtg aaatgcatag atatgactta
      181 gaacaccaat tgcgaaggca gcttactaag cagatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005860
VERSION     KHUT01005860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           81 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaaag
       61 tcagtcagat gtgaaatccc ggagctcaac tccggaacag catttgaaac ttcttttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttacacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01005861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005861
VERSION     KHUT01005861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           96 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggtgcgt
       61 tgcgtcagat gtgaaatccc cgggctcaac ctgggaatgg catttgatac gggcgtactg
      121 gaatccgtga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccaactggc acggtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005862
VERSION     KHUT01005862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtttg
       61 caagtccgtg gtgaaatcct ccagctcaac tggagaactg ccgcggatac tgtgagtctc
      121 gagggaggta ggggcgtgcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005863
VERSION     KHUT01005863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt ccgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttgc
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaactg ccgtggatac tggcgacctc
      121 gaggaagata ggggactctg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg agggtctgga tcttttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005864
VERSION     KHUT01005864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcactg
       61 taagtaagat gtgaaatctc cttgctcaac ggggagggtg cattttagac tacagagcta
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005865
VERSION     KHUT01005865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttgta
       61 taagttgttt gtgaaatccc cgggcttaac ctgggaactg caagcaatac tgtataacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005866
VERSION     KHUT01005866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           86 g           55 t
ORIGIN      
        1 tacggaggtt ccaagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggatagg
       61 tcagtttaaa gtggaatccc agggctcaac cctggacgtg ctttggatac tgcctgtctt
      121 gagttcgtga gaggaaagtg gaattcaggg tgtagcggtg aaatgcttag atatcctgag
      181 gaacaccagt ggcgaaagcg actttctggc acgaaactga cgctgaggct cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005867
VERSION     KHUT01005867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgctcgta ggcggatatt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg ccttcgaaac tgggtatctg
      121 gaggccgaga gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactggc tcggtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005868
VERSION     KHUT01005868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 taagctagat gtgaaatccc tgggctcaac ctgggaactg catttagaac tgctaggcta
      121 gagtgcagaa gaggagtgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg acactctggt ctgacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005869
VERSION     KHUT01005869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           76 g           61 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaatctc cgagcctaac tcggaaattg ccattgatac tgatggtctt
      121 gagtacattt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005870
VERSION     KHUT01005870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgctcgca ggcggtgatg
       61 tacgtagagg gttccagctg tcggcttaac cgacagagtg ctcgctaaac ggcatgactt
      121 gaggacgaga gagggacatg gaactccggg tggagtggtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtatctga cgctcaggag cgagagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005871
VERSION     KHUT01005871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           93 g           46 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctttg
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg catccgaaac tgcaaggctt
      121 gagtaccaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgatctggg tggaaactga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005872
VERSION     KHUT01005872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          106 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgggg
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgccctgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005873
VERSION     KHUT01005873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgtcg
       61 caagtccgtg gtgaaatctc taggctcaac ttagagactg ccgcggaaac tgcggtgctt
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005874
VERSION     KHUT01005874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           87 g           55 t
ORIGIN      
        1 tacgggggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaagtg
       61 caagtcagcg gtgaaatccc cgcgctcaac gcgggagttg ccgttgatac tgtacatctg
      121 gaatgctctt gccgtgggag gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaacaccgat tgcgaaggca tctcacgaag gggcgattga cgctgaggca cgaaagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01005875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005875
VERSION     KHUT01005875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           87 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggcagga
       61 taagttagat gtgaaatgtc gacgcttaac gtcgaacctg catttaagac tgtcttgctt
      121 gagcgctaga gaggagtctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaaggccagt ggcgaaagcg agactctggc tagatgctga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005876
VERSION     KHUT01005876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 cacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcaatt
       61 caagtcaggt gtgaaatgcc tgagctcaac tgaggcattg cgcttgaaac tgaactgctt
      121 gagtatccca ggggtgtgcg gaactgccag tggagcggtg aaatgtgttg atattggcag
      181 gaacaccgga ggcgaaagcg gcacactggg ggataactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005877
VERSION     KHUT01005877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggccat
       61 taagtcaggg gtgaaagacg gttgctcaac aatcgcagtg cctttgatac tggtggtctt
      121 gggtgacggt gaggtaggcg gaatgggtgg tgtagcggtg aaatgcatag ataccaccca
      181 gaactccgat tgcgaaggca gcctactggc cgttgaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005878
VERSION     KHUT01005878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtttgg
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tgccaagcta
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cagatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005879
VERSION     KHUT01005879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggaaat
       61 taagtcgggg gtgaaagacg gttgctcaac aatcgcagtg ccttcgatac tggttttctt
      121 gggtaaagat gaggtaggcg gaatggaagg tgtagcggtg aaatgcatag ataccttcca
      181 gaactccgat tgcgaaggca gcctactggg cttttaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005880
VERSION     KHUT01005880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           80 g           59 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggcttgg
       61 aaagtcagtg gtgaaatctg agagcttaac tctcaaactg ccattgatac ttcttagctt
      121 gagtacggaa gaggctgatg gaatttcagg tgtagcagtg aaatgcgtag atatctgaaa
      181 gaacaccagt ggcgaaggcg gtcagctggt ccgttactga cactaaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005881
VERSION     KHUT01005881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggactta ctgggcgtaa agggttcgta ggcgggagcc
       61 taagtagcgt gtgaaatccc cgggctcaac ccgggacgtg cacgctaaac tgggcttctt
      121 gagtatcgga gagggtcatg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgtaagcg atgacctggc cgaatactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005882
VERSION     KHUT01005882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcgatg
       61 taagtcggat gtgaaatccc tcggcttaac cgaggaagtg cattcgatac tgtgtcgctt
      121 gagtatcgga ggggtcggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccgactgga cgatcactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005883
VERSION     KHUT01005883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           48 t
ORIGIN      
        1 cacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggcgcgt
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaattg cgtccgaaac tggcgtgctt
      121 gagtgagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcaactga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005884
VERSION     KHUT01005884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           98 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttttg
       61 tgggtgagat gtgaaagccc tgggcttaac ctgggacggg cattttaaac tataagactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005885
VERSION     KHUT01005885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggtttcg
       61 tcagtcagat gtgaaagccc cgggcttaac ccgggaacag catttgatac tgcggaacta
      121 gagttcggga gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005886
VERSION     KHUT01005886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggtggtttgg
       61 taagtctgct gtggaagctc ccggcttaac tgggagaggt cagcggaaac tgccaggctt
      121 gagtgtggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ccactactga cactaagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005887
VERSION     KHUT01005887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           94 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggtgtaa agggtgcgta ggcggaggtt
       61 taagtctgtg gtgaaaggtt accgctcaac ggtagaatgg ccatggatac tggatctctt
      121 gagtgtgaca gaggcaggtg gaattcgtag tgtagcggtg aaatgcatag atattacgaa
      181 gaacaccagt ggcgaaggcg gcctgctggg tcacaactga cgctgaggca cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005888
VERSION     KHUT01005888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           58 c          107 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggccacg
       61 tgcgtcacgg gtgaaatctc ccggctcaac tgggaggggt cctgtgatac ggcgcggctc
      121 gagcgaggta gggggtcgtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcgacctgga cctcggctga cgctaaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01005889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005889
VERSION     KHUT01005889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtctgt
       61 tgtgtcggat gtgaaagccc tgggctcaac ctgggaattg cattcgaaac tggcaggcta
      121 gagttcgata gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gcgacctgga tcagaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005890
VERSION     KHUT01005890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          104 g           45 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggattta ttgggcgtaa agggtgggca ggtggccggt
       61 gatgtgccgt gtgaaagcgc cgtagtaatg acggcgaggt cgcggtagac acatcggctt
      121 gaggcagtga gggggcggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gccgcctggc actgacctga cactgagcca cgacggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005891
VERSION     KHUT01005891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaacgg cgcccaaaac tactgtgctg
      121 gaggaagaca gaggtgagcg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005892
VERSION     KHUT01005892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtcttg
       61 tttgtctagg gtgaaatccc agggcttaac cctggaagtg ccttagaaac ggcaggacta
      121 gagttctaga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga agcgaaggcg acgacctgga tagatactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005893
VERSION     KHUT01005893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          100 g           43 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 tgcgtcgggt gtgaaagccc tgggctcaac ctgggaggtg cactcgaaac gggcaggctc
      121 gaaaccgaga gaggatcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccaga ggcgaaggcg gcgatctggc tcggatttga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005894
VERSION     KHUT01005894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           42 c           95 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggtggatctt
       61 taagtcgtgt gtctaagtgc ggtgctcaac gccgtatggg cgcaggaaac tggagatctt
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtgtctga cactgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005895
VERSION     KHUT01005895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           98 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgt
       61 cgcgtcgatt gtgaaaaccc ggggctcaac tccgggcttg cactcgatac gggcggacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005896
VERSION     KHUT01005896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcttag
       61 caagtcggaa gtgaaagccc ttggctcaac caaggaagtg ctttcgaaac tgctgagctt
      121 gagtagttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aacatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005897
VERSION     KHUT01005897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          103 g           38 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtcacg
       61 caggtcatct gtgaaagcac tcggctcaac cgggatgttg cgggtgaaac cgcgtgactt
      121 gagggtggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt agcgaaggcg gccgactgga ccacacctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005898
VERSION     KHUT01005898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           38 c           81 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctcta
       61 taagtcagtg gtgaaagtct gcagcttaac tgtagaactg ccattgatac tgtggagctt
      121 gagtactgat gaggtaggcg gaattgatag tgtagcggtg aaatgcatag atattatcaa
      181 gaacaccgat tgcgaaggca gcttactaag gagttactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005899
VERSION     KHUT01005899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           82 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggatttg
       61 taagtcagtg gtgaaagcct ggagcttaac tccggaattg ccattgatac tgcttttctt
      121 gagtacagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01005900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005900
VERSION     KHUT01005900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           82 g           52 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggcggcttct
       61 caagtcaggg gtgaaatccc agagcttaac tctggaactg cctttgaaac tgagaagcta
      121 gaatgtgaga ggggatggcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacacccgt ggcgaaagcg gccatctgga tcacaattga cgctgaagta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005901
VERSION     KHUT01005901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtctgta ggcggtttta
       61 caggtctggc gtgacaattc tcggcttaac tgagagagtg cgttggaaac cggaagacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacccctaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005902
VERSION     KHUT01005902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          107 g           37 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccgtg
       61 taggcacgac gtgaaagccc cgggctcaac ccgggagggt cgtcgtgaac cgcatggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtgctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005903
VERSION     KHUT01005903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          100 g           51 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgt
       61 tgcgtctgcc gtgaaagccc acagcttaac tgtgggtctg cggtggatac gggcggactt
      121 gagtcctgta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caggtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005904
VERSION     KHUT01005904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           76 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtagg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccgttgatac tatttatctt
      121 gaatatcggg taggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttacttca agattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005905
VERSION     KHUT01005905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           93 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcctgg
       61 caagtcggat gtgaaagccc tcggctcaac cggggaagtg catccgaaac taccaggctc
      121 gagtgtcgga gagggaagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga cgaacactga cactgagacg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005906
VERSION     KHUT01005906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           98 g           37 t
ORIGIN      
        1 cacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agagcgtgta ggcggttcgg
       61 taagtcaacc gtgaaagctc ccggctaaac tgggagaggt cggatgagac tgccgggcta
      121 gagggtcgta gaggggtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcaccctgga cgacaccaga cgctgagacg cgacagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005907
VERSION     KHUT01005907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          100 g           42 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttgca
       61 caagtccgtt gtgaaagccc ctggctcaac tgggggaggt caatggagac tgtgcgactt
      121 gagggcagca gaggagcgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gcgctctggg ctgcacctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01005908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005908
VERSION     KHUT01005908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           93 g           42 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggaatta ctgggcgtaa agcgcaagca ggcggagctt
       61 taagtcggat gtgaaagcgc ctggctcaac taggcgaggc catacgatac tgaaggactc
      121 gagggtggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccaaccctga cgctcagatg cgaaagctag
      241 gggagagaac ggg
//
LOCUS       KHUT01005909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005909
VERSION     KHUT01005909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           96 g           49 t
ORIGIN      
        1 tacggagggt gcgaacgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttcgt
       61 taagtgggat gtgaaagccc tgggcttaac ctgggaatgg catttcagac tggcggacta
      121 gagtacggta gagggcggtg gaattccctg tgtagcggtg aaatgcgtag atatagggag
      181 gaacatcagt ggcgaaggcg gccgcctgga tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005910
VERSION     KHUT01005910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           92 g           52 t
ORIGIN      
        1 ttcgggtgag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggctcgg
       61 taagtacctt gtgaaatccc ttctctcaat gaaggaaccg cttggtatac tgccgggctg
      121 gaggcaggta ggggtcactg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcccgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01005911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005911
VERSION     KHUT01005911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          101 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggagggt
       61 taagtgagaa gtgaaaggct ggggctcaac cccaggagtg ctcctcatac tggccttctt
      121 gagtacgtca gggagaagtg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg gcgttactga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005912
VERSION     KHUT01005912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaataa ctgggcgtaa agagttcgta ggctgtgtcg
       61 caagtcagtg gtgaaatccc ggggcttaac ctcggaactg ccattgaaac tgcgacactg
      121 gaatctcaga ggggatagcg gaattcctaa tgtaggggtg aaatccgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga tgagtattga cgctgaggaa cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005913
VERSION     KHUT01005913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           98 g           41 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctgcg
       61 caagtctcgg gtgaaatccc tcggctcaac cgaggaactg ccctggaaac tgcgcggctc
      121 gaggccggga gaggatggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005914
VERSION     KHUT01005914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          104 g           39 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggccgca
       61 taagtcgggt gtgaaagccc ggggctcaac cccgggatgc cgcccgagac tgcgcggctt
      121 gaggacggaa gaggggagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccgtccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005915
VERSION     KHUT01005915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcgggccgt
       61 aaagtcaggg gtgaaatcct ccagcttaac tggagaactg cctttgatac tgacggtctc
      121 gagtgaggta ggggtgtgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaggcg gcacactgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005916
VERSION     KHUT01005916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           89 g           56 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtggactcc
       61 taagtcgggg gtgaaatccc gcagctcaac tgcggaattg ctttcgatac tgggggtctt
      121 gagtacggca gagggagata gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaataccggt agcgaaggcg gtctcctggt ccgttactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005917
VERSION     KHUT01005917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           90 g           44 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttac
       61 caagtcttct gtcaaagatc agggcttaac cctgggcagg caggagaaac taatagacta
      121 gagttcggta gaggcagagg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccaag ggcgaaggca ctctgctggg ccgcgactga cactgagaga cgaaagcgag
      241 gggagcaaat ggg
//
LOCUS       KHUT01005918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005918
VERSION     KHUT01005918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggctatg
       61 taagtctgct gttaaatagt catgctcaac atgatcaagg cagtggaaac tacaaggcta
      121 gagtgcgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01005919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005919
VERSION     KHUT01005919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggcttcg
       61 taagttggat gtgaaagctc ctggcttaac tgggagaggc cattcgagac tgcgaggctt
      121 gaggttggga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005920
VERSION     KHUT01005920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttag
       61 caagtcagac gtgaaatccc taggcttaac caaggaatgg cgtctgatac tgctagacta
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005921
VERSION     KHUT01005921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggagtga ttgggcgtaa agggtgcgta ggctgtgcgt
       61 taagtcaact gtcaaatttc ccagcttaac tgggagccgg cggttgaaac tggcgtgctt
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005922
VERSION     KHUT01005922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacagc
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaattg cccttgatac tggttgtctt
      121 gagatcggaa gaggtaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg acttactggt ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005923
VERSION     KHUT01005923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ttgggcgtaa agggcgcgta ggcggccttg
       61 ttagtcaggc gtgaaattcc tgggcttaac ctggggactg cgcttgatac ggcagggctc
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005924
VERSION     KHUT01005924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcct
       61 caagtcagtc gtgaaatctc cgggcttaac ccggaagcgt cgattgatac tgtcgagcta
      121 gagtgtggaa ggggctaccg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccggc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005925
VERSION     KHUT01005925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctatc
       61 taagtctggt gtgaaagccc agggctcaac cctggaagtg cactggatac tgggtagcta
      121 gaggacgaga gaggaaagtg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatctgt ggcgaaggcg actttctggc tcgtttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005926
VERSION     KHUT01005926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           87 g           51 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ctgggcgtaa agcgtacgta ggcggtggtt
       61 caagtcagag gtgaaagccc ggagctcaac tccggaactg cctttgaaac tagatcgcta
      121 gaacatcgga gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactgga cgattgttga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005927
VERSION     KHUT01005927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 tacagggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggtcttt
       61 taagtccttt gtgaaatccc ggggctcaac tccggatgtg cataggatac tgaaagactt
      121 gagacaggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg cctgttctga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005928
VERSION     KHUT01005928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggctgcc
       61 taagtctaat gtgaaatccc ggagcttaac tccggaactg catcggatac tgggcagcta
      121 gaggatgaga gaggatggtg gaattccagg tgtaggggtg aaatccgtag atatctggag
      181 gaacatcagt ggcgaaggcg gccatctggc tcatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005929
VERSION     KHUT01005929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           79 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatcct agggcttaac cctagaactg ccgttgatac tctctgcctt
      121 gaatcccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaca cgacgattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005930
VERSION     KHUT01005930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtcagag gtgaaatcct gcggcttaac cgcagaagtg cccctgatac tggtagtctc
      121 gaggggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005931
VERSION     KHUT01005931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcgggtcgt
       61 taagtcgggg gtgaaagccc agagctcaac tctggaactg ccttcgatac tggcgacctg
      121 gacccaagta gaggcgagtg gaattacgag tgtagaggtg aaattcgtag atattcgtag
      181 gaacaccagt ggcgaaggcg actcgctgga cttgtggtga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005932
VERSION     KHUT01005932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgca
       61 aaagtccggg gtgaaagccc ccagctcaac tggggaagtg cctcggatac tgtgcggctc
      121 gagcgaggca ggggtcgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacgactggt cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005933
VERSION     KHUT01005933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           87 g           51 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggcatat
       61 aaagtctgat gttaaagtcc ggggctcaac tccggtcaag cattggatac tgataagcta
      121 gagtgcagaa gaggcaaggg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgtaagcg ccttgctggg ctgtaactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01005934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005934
VERSION     KHUT01005934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacgtaggga gccagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggttttg
       61 ttagcggagg gtgaaatctc ggggctcaac tccgaggctg ctttccgaac tgcaagacta
      121 gagtacggga gaggatagtg gaattcctgg tgtagcggtg gaatgcgtaa atatcaggag
      181 gaacacccgt ggcgaaggct gctatctgga acgtaactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005935
VERSION     KHUT01005935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           81 g           56 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggtatgc
       61 taagtcaatt gttaaattct ccggcctaac cggaggcaaa taatagatac tggcgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005936
VERSION     KHUT01005936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 taagtcaggt gtgaaagccc tcggcttaac cgaggaagtg cgcctgaaac tgcagagctt
      121 gagtgccgga gagggcggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg accgcctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005937
VERSION     KHUT01005937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagataggc gtgaaatccc cgggctcaac ctgggaactg cgtttatgac tggcaggcta
      121 gagtatggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaatactga cgctcatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005938
VERSION     KHUT01005938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           50 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggagcg
       61 tgcgtctgaa aggaaatctc agggctcaac tctggggctt tttcagatac ggcgcttctt
      121 gagtgtaaca gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctggg ttaccactga cgctgaggag cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005939
VERSION     KHUT01005939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 gacagagggt gttaacgttg ctcggaatta ctgggtgtaa agcgcgtgca ggtggttgcg
       61 taagttcggt gtgaaagccc caggctcaac ctgggaagtg cactgaatac tgcgcgactt
      121 gagttctgaa gaggagagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg actctctggg cagatactga cactaaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005940
VERSION     KHUT01005940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           85 g           50 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacttg
       61 caagtcagat gtgaaatccc cgagctcaac tcgggaactg catttgaaac tgcctgtctt
      121 gagtagtgga gaggaaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005941
VERSION     KHUT01005941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtttgc
       61 caagtccggg gtgaaagcct ccagcttaac tggagaagtg ccttggatac tggcgaactc
      121 gagtgaggta ggggtgatgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg catcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005942
VERSION     KHUT01005942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggctcgt
       61 taagtcggtt gtgaaagccc atggctcaac catggaaggg cgtccgaaac tggcgaacta
      121 gagtcccgaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacgtcaga ggcgaaggcg gccatctggg agggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005943
VERSION     KHUT01005943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacgtatggt gcaagcgtta tccggattta ctgggtgtaa agggagcgca ggcggtgcgg
       61 caagtctgat gtgaaagccc ggggctcaac cccggtactg cattggaaac tgtcgtacta
      121 gagtgtcgga ggggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005944
VERSION     KHUT01005944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           67 c          104 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 acggtccgcg gtgaaatccc cccgctcaac gggggaacgg ccgcggatac cgccggcctc
      121 gaggggggta ggggcgagcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gctcgctgga ccttctctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005945
VERSION     KHUT01005945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatg
       61 tgagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gacatagctg
      121 gagtataaga gagggtgatg gaatttccgg tgtagtagtg aaatacgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gtcacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005946
VERSION     KHUT01005946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggctttg
       61 caagtcaggt gtgaaagcct ggggctcaac cccagaagtg catttgaaac tgcagagctt
      121 gagtcttgga gagggcagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actgcctgga caaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005947
VERSION     KHUT01005947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           87 g           45 t
ORIGIN      
        1 tacgaatcgt ccaaacgtta ctcggaatca ctgggcttaa agggtgcgta ggccgctcgg
       61 agggtggaat gtgaaatccc ccagcttaac tggggaatag cgtcccaaac cgccgggctt
      121 gagggagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gctcactggg tctttcctaa cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005948
VERSION     KHUT01005948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggcggttcag
       61 tatgtcggtc gtgaaatccc cgggcttaac ctgggaactg cggccgaaac tgctgggctc
      121 gagtgtggta gagggcggtg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacatcaat ggcgaaggca gccgcctggg ccacaactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005949
VERSION     KHUT01005949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggattca ctgggcgtaa agggcgcgta ggcggctcgg
       61 taagtttaat gtgaaagccc agggctcaac cctggaagtg cattgaatac tgccaagcta
      121 gagtactgga gaggtgggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacgccagc ggcgaaagcg gcccactgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005950
VERSION     KHUT01005950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           84 g           56 t
ORIGIN      
        1 tacgagtgcc tcaagcgtta tccggaatca ttgggcgtaa aggatgtgta ggtggttgtg
       61 ttagtctctt gttaaattct tcggcttaac cgggggcatg caagggaaac ggcacgacta
      121 gaggatgcga ggggtctgcg gaactcatag tgtagcggtg aaatgcgttg atattatggg
      181 gaacaccaaa agcgaaggca gcagactgga gcactcctga cactgaaaca tgaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01005951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005951
VERSION     KHUT01005951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcca
       61 caagtcacct gtgaaatctc ctggctcaac cgggagcttg caggcgaaac tgtggtgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005952
VERSION     KHUT01005952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           77 g           61 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaaatg
       61 taagtcagtg gtgaaagact gtcgcttaac gatagcattg ccattgatac tgcaagtctt
      121 gagtacattt gaggtgggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005953
VERSION     KHUT01005953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c           99 g           43 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatctc gcggctcaac cgcggaactg cctcagatac tgacggcctc
      121 gaggggggta ggggcgagcg gaacgggtgg tggagcggtg aaatgcgttg atatcactcg
      181 gaactccggt ggcgaaggcg gctcgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005954
VERSION     KHUT01005954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           95 g           39 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtgcctc gtgaaatccc acggctcaac cgtggaaccg cggggcatac tggcgatctt
      121 gagagaccca ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtcatctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005955
VERSION     KHUT01005955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           87 g           47 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaagcg
       61 taagtgcttt gtgaaatccc acggcttaac cgtggaactg cttggcatac tgcgtttctt
      121 gagccactca ggggtcgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggcgactggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005956
VERSION     KHUT01005956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           94 g           47 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggagtta ctgggcgtaa agggcgttta ggcgggtgat
       61 taagttcgct atgaaagcgc caggctaaac ctggcgaggc tggcggatac tggtcatctg
      121 gagtaaggaa gagggaggta gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaataccagt ggcgaaggcg gcctcctggt ccttaactga cgctgaagcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU5999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005957
VERSION     KHUT01005957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU5999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 cacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtttgt
       61 tacgtccgct gtgaaaacct agggcttaac cctgggcttg cagtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cactgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01005958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005958
VERSION     KHUT01005958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          100 g           47 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggctctt
       61 taagtatggc gtgaaatctc cctgctcaac ggggagggtg cgttgtagac tgaggagctt
      121 gaggtcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgacc cgg
//
LOCUS       KHUT01005959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005959
VERSION     KHUT01005959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggtccgg
       61 taagtttgga gtgacagtgc tcggcttaac tgagcaaggt cttcaaaaac tgctggactt
      121 gaggacttga gagggacata gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggc aagtacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005960
VERSION     KHUT01005960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggctttg
       61 ttagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tgcaaggcta
      121 gagtactgga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga cagtaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005961
VERSION     KHUT01005961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 gacgaaggtg acgaccgttg ctcggaatta ctgggcataa agcgcgcgta ggtggcaggg
       61 taagtccatg gtgaaatccc tcggctcaac cgaggaagtg ccgtggatac tgctctgctt
      121 gaggacggga ggggtgagtg gaactctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacgccggt ggcgaaagcg actcactgga ccgtttctga cactgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01005962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005962
VERSION     KHUT01005962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggtctaa
       61 caagtctggc gtgaaatcct ctggcttaac caggggcggt cgctggagac tgttaaactt
      121 gaggcgatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctggc gtcgtactga cgcacatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005963
VERSION     KHUT01005963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 aacgtaggtc ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtctag
       61 caagtcgggc atgaaatctc tcggctcaac cgggaggggt tgtccgatac tgctggactt
      121 gagggcagga gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccacctggc ctgcacctga cgctgaaacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005964
VERSION     KHUT01005964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           90 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggctgttagc
       61 taagtcgggt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac tagctagcta
      121 gagagtggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctggg ccacttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005965
VERSION     KHUT01005965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c           99 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggttcgt
       61 taagtctagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcggactt
      121 gaggggggta gaggagagtg gaattctcgg tgtagtggtg atatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gctctctggg ccctacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005966
VERSION     KHUT01005966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           72 g           67 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttta
       61 taagtcagtg gtgaaatccg gcagctcaac tgtcgaactg ccattgatac tgtagaactt
      121 gaatgattgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccgat tgcgaaggca ggttactaac aatttattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005967
VERSION     KHUT01005967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           99 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgtta cccggattta ttgggcgtaa agagcgcgta ggcggttgtc
       61 cacgtcggat gtgaaagctc gaggctcaac cttgagaggt cattcgatac gggacggcta
      121 gaggcaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01005968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005968
VERSION     KHUT01005968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           86 g           58 t
ORIGIN      
        1 gacgaggggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggtatat
       61 taagtctgtt gttaaatctc ccggctcaac cgggaatatg cggtggatac tggtgtacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005969
VERSION     KHUT01005969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacatt
       61 taagtcaggg gtgaaagccc agagctcaac tctggaatgg cctttgatac tgggtgtctc
      121 gagtctggta gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccagtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005970
VERSION     KHUT01005970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           75 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcctta
       61 taagtcagtg gtgaaatctc ccggctcaac cgggaaattg ccattgatac tgtagggctt
      121 gaattattgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactacc aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01005971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005971
VERSION     KHUT01005971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c           97 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggactgg
       61 ttagtccggg gtgaaagctc atggctcaac tgtgagtggt ccccggatac tgcccgtctt
      121 gaggggtcta gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gctctctggg gatcacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01005972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005972
VERSION     KHUT01005972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           73 g           62 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactta
       61 taagtcagtg gtgaaatctc caggcttaac ctggaaactg ccattgaaac tgtttgtctt
      121 gagtacattt gaggcgggcg gaatgtgtca tgtagaggtg aaattcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgtaactga cgctgatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005973
VERSION     KHUT01005973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggttatt
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaagtg catccgaaac taactagcta
      121 gaagatggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ccatccttga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005974
VERSION     KHUT01005974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatttgtctt
      121 gaataccgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgcctattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01005975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005975
VERSION     KHUT01005975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c          100 g           41 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggatctg
       61 taagtgggtg gtgaaagcgc ccggctaaac cgggcgaggt ccattcagac tgcaggtcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaagca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005976
VERSION     KHUT01005976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           75 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtccgtg gtgaaatcct atagcttaac tatagaactg ccatggatac tatttgtctt
      121 gaatgttgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gcttactacg caaatattga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005977
VERSION     KHUT01005977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          100 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggactta ctgggcgtaa agcgcgcgca ggtggacgag
       61 tatgtgccgt gtgaaagcgc catccttacg atggcgaggt cgtggtagac agcttgtcta
      121 gaggctcgaa gaggtcgatg gaattccggg tggagcggtg aaatgcgtag atatccggag
      181 gaacaccagg ggcgaaggcg gtcgactggt cgagttctga cactgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01005978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005978
VERSION     KHUT01005978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           85 g           48 t
ORIGIN      
        1 tacgagttgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctgcg
       61 aaagtcagat gtgaaatccc tcggctcaac cgaggaactg catttgaaac tgcgcagcta
      121 gaggaagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttatctga cgctgatgca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005979
VERSION     KHUT01005979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          101 g           44 t
ORIGIN      
        1 gacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggcggattgg
       61 taagtggttc gtgaaatatc cgggctaaac tcggagggga cgaatcagac tgctagtctg
      121 gaggcgggta gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag atatccggag
      181 gaacaccaga ggtgcagacg gcgccctggg cccgacctga cgctgagacg cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01005980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005980
VERSION     KHUT01005980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           98 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcccgc
       61 taagtcggat gtgaaatccc gcggctcaac cgcggaactg cactcgatac tggtgggctt
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005981
VERSION     KHUT01005981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           88 g           48 t
ORIGIN      
        1 gacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttgat
       61 gaagtctact gttaaatatt agggcttaac cctgggaagg cagtagaaac tcattggcta
      121 gagtacggta ggggcagagg gaattcccgg tgtagcggtg aaatgcggag atatcgggaa
      181 gaacaccaat ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005982
VERSION     KHUT01005982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggtgctc
       61 taagtctgtc gtgaaatctc ccggctcaac tgggagggtg cggcggaaac tggagtgctc
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccacgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005983
VERSION     KHUT01005983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacatt
       61 taagtcagag gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgggtgtcta
      121 gagtatggaa gaggtaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gcttactggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005984
VERSION     KHUT01005984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgcgta ggcgggcagg
       61 taagtcggtt gtgaaatccc tgggctcaac ctaggaactg caatcgaaac tacctgtctt
      121 gagtgacgga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgtccactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005985
VERSION     KHUT01005985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           53 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatta ttgggtttaa agggtgcgca ggcggggatc
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tggattcctt
      121 gaatatacct gaggtaggcg gaatgtggca agtagcggtg aaatgcatag atatgccaca
      181 gaacaccgat tgcgaaggca gcttaccaaa gtatgattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01005986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005986
VERSION     KHUT01005986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           97 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttct
       61 taagtccgtt gtgaaagccc tgggctcaac ctgggaatgg cgatggatac tgaggagctg
      121 gagtgcggta gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccctctgga ccagcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005987
VERSION     KHUT01005987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          102 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcacgg
       61 tcagcgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccatgcgaac tgccgagctt
      121 gagcactgta gaggcagacg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005988
VERSION     KHUT01005988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgg
       61 taagtcgggg gtgaaatccc ggggctcaac cccggaactg cctccgaaac ttccagtctg
      121 gaggtcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005989
VERSION     KHUT01005989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           43 c           94 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttgct
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tgagtgacta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctctctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01005990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005990
VERSION     KHUT01005990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           97 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggagtta ctgggcgtaa agggcgcgta ggcggccctc
       61 caagtcgggt gtgaaagccc acggctcaac cgtggaattg cactcgagac tgaagggctc
      121 gaggacggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005991
VERSION     KHUT01005991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctgcg
       61 caagtcggat gtgaaacccc gaggctcaac ctcggaactg catccgatac tgcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcatgtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005992
VERSION     KHUT01005992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtccgta ggcggtcggc
       61 taagtcggat gtgaaatctc gtggcctaac cacgagcggt cattcgaaac tagtcggcta
      121 gagggctgga gaggagactg gaattgtcgg tgtagcggtg aaatgcgtag agatcgacaa
      181 gaacaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01005993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005993
VERSION     KHUT01005993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           89 g           46 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgtgcgta ggcggctctt
       61 caagtgcttt gtgaaagccc tcggctcaac cgaggaattg cttggcatac tggggagctt
      121 gaatcatcta ggggcagctg gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaacgccaac ggcgaaagca ggctgctggg gatgcattga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01005994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005994
VERSION     KHUT01005994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          105 g           35 t
ORIGIN      
        1 gacggaggag gcgagcgtta tccggattta ctgggcgtca agggcgcgta ggcggcctag
       61 caggtcgtgg gtgaaagccc caggctcaac ctggggaggt cctgcgatac cactaggctt
      121 gaggatcgta gaggggggcg gaatgtccgg tggagcggtg gaatgcgtag atatcggacg
      181 gaacacccga ggcgaaggcg gccccctggg cgacacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaag cgg
//
LOCUS       KHUT01005995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005995
VERSION     KHUT01005995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacggggggt gcgaacgttg ttcggaatca ctgggcgtaa agagcgcgta ggcggtcggg
       61 taagtgaggt gtgaaatcca tcggctcaac cgatgaactg cacttcatac tgtccggctt
      121 gagggcagca gaggagacgg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cgtctctggg ctgcacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01005996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005996
VERSION     KHUT01005996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacggggggt gcaagcgtta ctcggaatca ttgggcgtaa agggcgcgta ggcggcctgg
       61 taagtcaggt gtgaaagccc ggggctcaac cccggaagtg cacttgatac tgccaggctt
      121 gagtatggga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctgga ccaatactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005997
VERSION     KHUT01005997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           92 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggtgctt
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgaggtgctc
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtagacg gcttactgga ccattactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01005998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005998
VERSION     KHUT01005998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           95 g           44 t
ORIGIN      
        1 aacgtaggtc ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtgggc
       61 taagtcaggc atgaaatctc tcggctcaac cgggagaggc tgtctgagac tggcgcactt
      121 gagggcagtc gagggaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaagcg gcctcctggg ctgcccctga cgctgacacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01005999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01005999
VERSION     KHUT01005999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           85 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggc
       61 taagtccggt gtgaaatctc ggagctcaac tccgaaactg cattggatac tattcggcta
      121 gagggttgga agggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaagca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006000
VERSION     KHUT01006000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           93 g           49 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggctgtcttg
       61 tatgtctgct gtaaaagcac acagctcaac tgtgtaaagt cagtggaaac tgtgagactt
      121 gaggctggga gaggctggtg gaattccctg tgtagcggtg aaatgcgtag atatagggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagacctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006001
VERSION     KHUT01006001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          103 g           41 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggctcga
       61 caagtcgtgt gtgtaagccc ccggctcaac tgggggaggt catgcgagac tgtggggctt
      121 gagggcagca gaggagcgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gcgctctggg ctgcaactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006002
VERSION     KHUT01006002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           79 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtatg
       61 taagtcagtg gtgaaatcct tgagcttaac tcgagaactg ccattgatac tatatgtctt
      121 gaatatcctg gaggtctgcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacacctat tgcgaaggca gcaggctacg ggatgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006003
VERSION     KHUT01006003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           88 g           47 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaactg cttggcatac tggcgatctt
      121 gagctgccta ggggtcactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgcccaa ggtgaaaaca ggtgactggg ggcaagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006004
VERSION     KHUT01006004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           79 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggctatt
       61 taagtcagtg gtgaaagccc gcagcttaac tgtggaattg ccattgaaac tgagtagctc
      121 gaatctggtt gaagtgggcg gaatacatca tgtagcggtg aaatgcatag atatgatgta
      181 gaacaccaat tgcgaaggca gctcactaag ccaacattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006005
VERSION     KHUT01006005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           35 c           68 g           69 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtggataat
       61 taagtcagag gtgaaatcct gcagctcaac tgtagaattg cctttgatac tggttatctt
      121 gagttattat gaagtagtta gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaataccaat tgcgaaggca gattactaat aatatactga cactgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006006
VERSION     KHUT01006006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           40 c           84 g           63 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccaat
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tggttggctt
      121 gagtgaagtc gaagtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gtttactagg ctttaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006007
VERSION     KHUT01006007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggggag
       61 caagtccgct gtgaaagccc gaggcttaac ctcggagggt cggtggatac tgcttctctt
      121 gaagaggcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctgga gccctcttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006008
VERSION     KHUT01006008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttggg
       61 caagtctgat gtgaaagccc cgggcccaac ccgggaacgg cattagaaac tgcctggctg
      121 gaggtttgga ggggggactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacgccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01006009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006009
VERSION     KHUT01006009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggctgctcag
       61 taagtcagtt gtgaaatccc tcggctcaac cgaggaactg cgtctgatac tgctgggctt
      121 gagtcccgga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga cgggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006010
VERSION     KHUT01006010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtcgg
       61 tccgtctggg gtgaaatcct ctcgcttaac gagagaacgg ccctggatac caccgatctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006011
VERSION     KHUT01006011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           99 g           42 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcccat
       61 caagtcaggc gtgaaattcc cgggctcaac ctggggactg cgcttgagac tggcgggcta
      121 gaggccgaga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccacctggc tcggtactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006012
VERSION     KHUT01006012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           89 g           44 t
ORIGIN      
        1 gacgaaccgt acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac tgccgtgctt
      121 gagggagaca gaggtgagcg gaactaaggg tggagcggtg aaatgcgttg atatccttag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006013
VERSION     KHUT01006013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 atagtctgcg gtgaaatccc tcagcttaac tggggaactg ccgtggatac tgccaatctc
      121 gaggagggta ggggcatctg gaacagccgg tggagcggtg aaatgcgttg atatcggctg
      181 gaactccggt ggcgaaggcg agatgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006014
VERSION     KHUT01006014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttgtg
       61 taagtccgct gttaaagccc ccggctcaac tgggggaggt cagtggagac tactcgactt
      121 gagggcaaca gaggcacatg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg atgtgctggg ttgcgactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006015
VERSION     KHUT01006015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaga
       61 taagttatct gtgaaatccc cgggcttaac ctgggcaggt cagataaaac tgtttgactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006016
VERSION     KHUT01006016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           46 c          102 g           56 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtctgcc atgtaatcct gcggcttaac tgtggggttg tggttgatac gggcaggctt
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006017
VERSION     KHUT01006017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctggg gtgaaatccc acggctcaac cgtggaactg cctcggatac tggcggcctc
      121 gagggaggta ggggcatgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006018
VERSION     KHUT01006018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtccgct gtgaaagtcc gaggctcaac ctcggaatgt cggcggaaac cccctggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006019
VERSION     KHUT01006019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           53 c          103 g           46 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggactta ctgggcgtaa agggtgggta ggtggtcggt
       61 gatgtgccgt gtgaaagccc cgcgttcatc ggggggaggt cgcggtagac acatcgactt
      121 gaggcattga gggggcggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gccgcctggc aatgacctga cactgagcca cgacggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006020
VERSION     KHUT01006020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          100 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctttg
       61 taagtcggac gtgaaatccc acggcttaac tgtggggcgc cgttcgatac tgcgaggcta
      121 gaggccggta gggggacgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgtcctggg ccggtcctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006021
VERSION     KHUT01006021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 ccagtcaggg gtgaaagccc ccagctcaac tggggaagtg cctttgatac tgccggcctt
      121 gaggggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006022
VERSION     KHUT01006022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttcg
       61 taagtccgtt gtgaaagtcc agggctcaac cctggaatgc cgatggatac tgcggagcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006023
VERSION     KHUT01006023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccgg
       61 taagtctgcc gtgaaagccc ggggctcaac cccgggatgc cggtggatac tgtcgggctt
      121 gagtccggaa gaggccagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat tgcgaaggca gctggctggg acggcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006024
VERSION     KHUT01006024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           89 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggtggtgctc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg catcgaaaac tggagtgcta
      121 gaggatggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttgctgga ccatttctga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006025
VERSION     KHUT01006025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           88 g           44 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggttctg
       61 aaagtctgat gtgaaatctc cgggcccaac ccggaaacgg cattagaaac tacagggcta
      121 gaggcttgga gggggaactg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacaccagt ggcgaaggcg agttcctgga caagtcctga cgctgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01006026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006026
VERSION     KHUT01006026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtttgg
       61 taagtgggat gtgaaatgcc tcggctcacc cgaggaccgg catcccaaac tgccaagctt
      121 gagtatggga gaggatgtgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cacatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006027
VERSION     KHUT01006027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           84 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggccgcgcgg
       61 gcagtcaaat gtgtaattca ggggctcaac ccctggactg cgcttgatac ttccgtgcta
      121 gaactttgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacactaag ggcgtaagca agactctgga caaatgttga cgctcatgca cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006028
VERSION     KHUT01006028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggaaac tggatgtcta
      121 gagggaggta ggggcctgtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg acgggctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006029
VERSION     KHUT01006029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           65 c          102 g           33 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcccgca ggcggccgtg
       61 cgcgtggggt gtgaaagctc gcggcttaac tgcgagaggc catcccatac ggcacggctc
      121 gagccgtcca gaggccggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccggctggg gacgtgctga cgctcagggg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006030
VERSION     KHUT01006030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggtggcgcgg
       61 caagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgccgtgcta
      121 gagtgctgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg gatctctgga cagctactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006031
VERSION     KHUT01006031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caggtccggg gtgaaagcct ccagctcaac tggagaagtg ccttggatac cggcggcctc
      121 gagggaggta ggggcgtgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006032
VERSION     KHUT01006032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 taagttcggt gtgaaatctc ggagcttaac tccgaaaagt cattgaatac tatccggctt
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006033
VERSION     KHUT01006033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          106 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgcgta ggtggacggc
       61 caagtctgtg gtgaaatcgc ccggctcaac tgggcgaggt ccgtggaaac tggtcgactg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006034
VERSION     KHUT01006034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggcttat
       61 caagtctgct gtcaaagcgc ggagcttaac tccgtaaagg cggtggaaac tgatgagcta
      121 gagtgtggta ggggtagcgg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagc ggcgaaagcg cgctactggg ccataactga cactgatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01006035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006035
VERSION     KHUT01006035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          104 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 caagtcggat gtgaaagccc ggggctcaac ctcggaggtg catccgagac tggcgtgctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006036
VERSION     KHUT01006036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           88 g           46 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtcaggt gtgaaatccc ggggcttaac cccggaactg cgcctgaaac tacatggcta
      121 gagtattgga gagggtagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctacctgga caattactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01006037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006037
VERSION     KHUT01006037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacccg
       61 cccgtccggg gtgaaagctt tcggctcaac cggaaaagtg ccttggatac cgcgggtctg
      121 gagggaggta ggggcatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006038
VERSION     KHUT01006038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttg
       61 caagtgcttt gtgaaatccc acggctcacc cgtggaatag cttggcatac tgcgagtctt
      121 gagccatcta ggggcaactg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg gatgtgctga cgctgagaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006039
VERSION     KHUT01006039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           90 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggccacg
       61 caagtcaggt gtgaaagccc agggctcaac cctggaagtg cacatgatac tacgtggctg
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006040
VERSION     KHUT01006040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttt
       61 taagtgcctt gtgaaatccc acggcttaac cgtggaatgg ctgggcatac tgggagtctt
      121 gagctgtcta ggggcgactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtcgctggg gacatgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006041
VERSION     KHUT01006041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggctcag
       61 tgagtcggat gtgaaagccc tgggcttaac ctgggaatgg cattcgatac ggctgggcta
      121 gagtatggta gagggaagtg gaatttccgg tgtaacggtg aaatgtgtag agatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccaatactga cgctcaagcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006042
VERSION     KHUT01006042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggcttgt
       61 cgcgttgtcc gtgaaaattt acagctcaac tgtgaacttg cgggcgatac gggcaggcta
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006043
VERSION     KHUT01006043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           97 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtg
       61 caagtctgcg gtgaaagcct ccagctcaac tggagaagtg ccgtggatac tgctcacctc
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006044
VERSION     KHUT01006044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgca ggcggtcttt
       61 taagtctgat gtgaaatctc gtagcttaac tacgagcggt cattggaaac tggaagactt
      121 gaggacagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006045
VERSION     KHUT01006045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           84 g           57 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcgtcctga
       61 caagttttag gtgaaatgcc caggctcaac ctgggagctg cctgaaaaac tgttgggatt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcag
      181 gaacaccaat agcgtaggca gcttactaga ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006046
VERSION     KHUT01006046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ctcggaatca ttgggcgtaa agagcgtgta ggtggctaga
       61 taagtcgggt gtgaaatccc ttggctcaac caaggaagtg cattcgaaac tatctagctc
      121 gaggatggca gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ccattcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006047
VERSION     KHUT01006047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           90 g           51 t
ORIGIN      
        1 gacggaggtc cctagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcgggatgg
       61 caagtacctt gtgaaatccc cggtctcaac ccgggaactg ctcggtatac tgtcattctt
      121 gaggcaggta ggggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01006048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006048
VERSION     KHUT01006048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c          103 g           35 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgca ggcggcctgg
       61 tgcgtcgaca ctgaaagcgc cccgcttaac ggggcgaggc gtggcgatac gactaggctc
      121 gaggcgtgga gagggtgggg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg cccacctggc cacgacctga cgctgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006049
VERSION     KHUT01006049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c           97 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcgtct gtgaaaactc agggcttaac cctgagcttg caggcgatac gggctggctt
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006050
VERSION     KHUT01006050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c           97 g           46 t
ORIGIN      
        1 tacgtaggtg gcaaacgttg tccggattca ctgggcgtaa agcgcgcgca ggcggattgg
       61 tcagtctggg gtgaaatctc tcggctcaac tgagagaggt ccctggatac tgccagtctt
      121 gaggggctca gaggagagtg gaattccgcg tgtagtggtg gaatgcgtag atacgcggag
      181 gaacaccagt ggcgaaagcg gctctctggg ggccacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006051
VERSION     KHUT01006051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcg
       61 taagtctgtg gtgaaagccc ccagcttaac tggggaagtg ccatggatac tgcgagtctt
      121 gaggagggta ggggcatctg gaacactggg tggagcggtg aaatgcgttg atatccagtg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006052
VERSION     KHUT01006052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           98 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgagta ggtggcggga
       61 taagtgtgtg gtgaaagctc ggggctcaac cccgagtctg ccatgcagac tgtcttgctt
      121 gagcgtagta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctactgctga cactgaatca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006053
VERSION     KHUT01006053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagttcgta ggcggtgcgg
       61 caagttcggt gtgaaatctc ccggctcaac tgggagggtg cgctaaatac tgccgtgctc
      121 gagtatagga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ctataactga cgctgaggaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006054
VERSION     KHUT01006054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           98 g           37 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggcccga
       61 caagtctgcg gtgaaagcct cccgcttaac gggagaaggg ccgtggaaac tgtcgggctc
      121 gagggagaca ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg agatcggaag
      181 gaactccggt ggcgaaagcg gcctgctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006055
VERSION     KHUT01006055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           85 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca gacggttatc
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaattg catttgaaac tgaatagcta
      121 gggtacggaa gagggaagcg gaatttcagg tgtagcagtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaggcg gcttcctggt ccagtaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006056
VERSION     KHUT01006056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           86 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggtttag
       61 atagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacggatac ttctaggcta
      121 gagtgtggta gaggaaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtttctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006057
VERSION     KHUT01006057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggtggtccct
       61 taagtcggat gtgaaagccc tcggctcaac tgaggaagtg catccgaaac tgaggggctg
      121 gagtatggta gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg ccaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006058
VERSION     KHUT01006058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcattg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcagtgctt
      121 gagtcctgga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccacctgga cagagactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006059
VERSION     KHUT01006059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa aggggttgta ggcggccaac
       61 taagtccgat gtgaaatccc aaagcttaac tttggaactg cgtcggatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgacattga cgctctaaac cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006060
VERSION     KHUT01006060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggcctgg
       61 taagtcagcg gtgaaatacc cacgctcaac gtgggaactg ccgttgatac tgccgggctt
      121 gagttcgtgc gaggtcgctg gaatgcgtgg tgtagcggtg aaatgcatag atatcacgca
      181 gaacaccgat tgcgaaggca ggtgactggc acgcgactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006061
VERSION     KHUT01006061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgca ggcgggacct
       61 taagtcagtg gtgaaatccc ttcgcttaac gaaggaactg ccattgatac tgaggttctt
      121 gaatctagtt gacgttagtg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat cgcgaaggca gctaacgaag ctagtattga cgctcaggca cgacagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006062
VERSION     KHUT01006062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           97 g           47 t
ORIGIN      
        1 aacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggtcttc
       61 taagtcaggc gtgaaagacc agggcttaac cgtgggattg cgtttgagac tggaaggctt
      121 gaggatcgga gagggcagtg gaatttccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctgcctgga cgacccctga cgctgaggcg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006063
VERSION     KHUT01006063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           79 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtat agggcgcgta ggcggactaa
       61 caagtcggat gtgaaatcct agggctcaac cctagaactg catccgaaac tgttagacta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01006064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006064
VERSION     KHUT01006064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          101 g           42 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggctgtt
       61 caagtggctc gtgaaagacc ccggctcaac cgggggtggt cgggtcagac tggatagctc
      121 gaggatgtca gagggacacg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaggcg gtgtcctggg gcgtacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006065
VERSION     KHUT01006065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c          100 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggcctgg
       61 taagtcgggg gtgaaaactc agggctcaac cctgagactg ccttcgatac tgctgggctt
      121 gagtgctcta gagggtagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg actgcctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006066
VERSION     KHUT01006066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgca ggcggatttg
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaattg ccattgatac tataagtctt
      121 gaattttgtg gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccaat tgcgaaggca gcaggctaca catgaattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006067
VERSION     KHUT01006067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           93 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttatct gtgaaagccc tgggctcaac ctgggaaggc cagataagac tgtgagactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006068
VERSION     KHUT01006068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           89 g           56 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatga ctgggcgtaa agagcacgta ggtggttaat
       61 ttagtcaggc gtgaaagccc tgggcttaac ccaggaattg cgcttgatac tggttaactt
      121 gagtccgata ggggaaagtg gaattcctag tgtagaggtg gaattcgtag atattaggag
      181 gaacaccaga ggcgaaggcg gctttctgga tcggtactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006069
VERSION     KHUT01006069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           85 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggagttc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggttctctt
      121 gaggtaccta ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaac ggtgaaaaca gctcgctggg ggtatcctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006070
VERSION     KHUT01006070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           88 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgagta ggtggtttag
       61 taagttaggt gtgaaagtcc tgggcttaac ctggggattg cgcctaatac tgcaaagcta
      121 gagtactgta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006071
VERSION     KHUT01006071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcccgca ggcggcttgt
       61 taagttcaag gtgaaatctc ccggctcaac tgggagggtg cctcgaaaac tggcaggctt
      121 gaggtgagga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cttatcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006072
VERSION     KHUT01006072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgg
       61 taagttgggg gtgaaatccc agggctcaac cctggaactg cctccaaaac tcccagtctg
      121 gagtccgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006073
VERSION     KHUT01006073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggtcttt
       61 tacgtcaggg gtgaaagccc ggggctcaac ctcgggagtg cctttgatac gggaagactt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg ccttgctggc cgatgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006074
VERSION     KHUT01006074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          100 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgcgta ggcggccgtt
       61 cacgtcgggt gtgaaagctc ggggctcaac cctgagaggt cattcgatac ggatcggcta
      121 gaggcaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01006075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006075
VERSION     KHUT01006075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           92 g           47 t
ORIGIN      
        1 tacgtaggtc acgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggcttaa
       61 taagttacgg ctgaaatccg gcggctcacc cgtcgaactg gccgtgatac tgttgagctt
      121 gaggtgcgga gggatgcgtg gaacttgtgg tggagcggtg aaatgcccag atatcacagg
      181 gaacaccggt ggcgaaggcg acgcattggc cgcaatctga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006076
VERSION     KHUT01006076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtcgac
       61 caagtctggg gtgaaatctt tcggctcaac cggaaaagtg cccccgatac tggccggcta
      121 gagggagata ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006077
VERSION     KHUT01006077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ctcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcagga gtgaaagccc tgggctcaac ccaggaagtg cttttgatac tgcgaagctt
      121 gagtgtggga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ccaacactga cactcagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01006078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006078
VERSION     KHUT01006078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           87 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctcta
       61 taagtcggat gtgaaagccc tcggctcaac caaggaagtg cattcgaaac tgtagagctt
      121 gagtagttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aacatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006079
VERSION     KHUT01006079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           84 g           52 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttagg
       61 taagtcaaat gtgaaatcct gcagctcaac tgcagaactg cattcgaaac tgcctggcta
      121 gagtactgga gaggagatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006080
VERSION     KHUT01006080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattca ctgggcgtaa agcgcgtgca ggtggtttga
       61 taagtcgggc atgaaaactc ttggctcaac tgagagacgc tgtccgatac tgttggactt
      121 gagggtgtaa gagggaggtg gaattccgca tgtagtggtg aaatgcgtag atatgcggag
      181 gaacaccagt ggcgaaggcg gcctcctggt acactcctga cactcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006081
VERSION     KHUT01006081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggcctgt
       61 taagtcgggg gtgaaaaccc ggagctcaac tccgggcctg cctccgaaac tggcgggctt
      121 gagtgctcta gagggcagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg gctgcctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006082
VERSION     KHUT01006082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           90 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttagct gtgaaagccc cgggcttaac ctaggaattg catctaagac tgttagactt
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006083
VERSION     KHUT01006083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 caagtcaggc gtgaaatccc ttggcttaac caaggaattg cgtttgaaac tgccaggcta
      121 gagtatcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gccacctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006084
VERSION     KHUT01006084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           88 g           43 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcccgg
       61 aaagttgtgt gtgaaagccc tcggctcaac cgaggaatag cgcgcaaaac taccgggctt
      121 gagggagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagg agcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006085
VERSION     KHUT01006085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggaaaa
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tgtttttcta
      121 gagtttgaca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgacctggg ttaaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006086
VERSION     KHUT01006086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agcgtgcgca ggcggtttgg
       61 taagtctgtc gtgaaatccc cgggctcaac ctgggaatgg cgatggagac tgccaggcta
      121 gaatttggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctcagattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006087
VERSION     KHUT01006087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           82 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggatatg
       61 taagtcagat gtgaaattcc gaaactcaat ttcggagctg catttgaaac tgcaaatctc
      121 gaggattggc ggagagaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaagcg gttctctagc ttatacctga cgctgaggcg cgagagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01006088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006088
VERSION     KHUT01006088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccgc
       61 caagtctggg gtgaaatctt tcggctcaac cggaaaattg cctgggatac tggcgggctc
      121 gagggaggta ggggtcgtgg gaactaccag tggagcggtg aaatgcgttg atattggtag
      181 gaacgccggt ggcgaaagcg cacgactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006089
VERSION     KHUT01006089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggattct
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgggagtctt
      121 gagccatcca ggggttaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtaactggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006090
VERSION     KHUT01006090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           40 c           93 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttatt
       61 taagttgact gtgaaatacc tgggcttaac ctgggttggt cagttaatac tggatgactt
      121 gagtagggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctgctactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006091
VERSION     KHUT01006091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           82 g           51 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggcctgg
       61 aaagtaaggt gtgaaatccc acagctcaac tgtggaactg cgctttaaac ttcccggcta
      121 gagggatata gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006092
VERSION     KHUT01006092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           84 g           55 t
ORIGIN      
        1 tacggaaggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttta
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaattg catctgatac tgtatgacta
      121 gagtatgcta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaat ggcgaaggca acttcctggg gcaatactga cactgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006093
VERSION     KHUT01006093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggagtat
       61 taagttagat gtgaaatccc tgggcttaac ctgggaatgg catttaatac tgataatcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006094
VERSION     KHUT01006094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           76 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttga
       61 taagtcagtg gtgaaagccc atcgctcaac gatggaacgg ccattgatac tgtcagactt
      121 gaattactag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactact agtggattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006095
VERSION     KHUT01006095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttatt
       61 caagtcagag gtgaaagccc agtgctcaac actggaactg cctttgaaac tagatagctt
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006096
VERSION     KHUT01006096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           82 g           59 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggcggctttt
       61 caagtcaggt gtgaaatccc atggcttaac catggaactg catttgaaac tgtaaagctt
      121 gagtattgga gaggatggta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gccatctggc caaatactga cgctgagata cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006097
VERSION     KHUT01006097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctcgg
       61 tcagtcgggt gtgaaatccc tgggctcaac ctgggaactg cattcgatac tgccgagcta
      121 gagtgtggga gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accgcctggc ccaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006098
VERSION     KHUT01006098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctttg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgcaaggcta
      121 gaggatgaga gaggaaagtg gaattacgag tgtagaggtg aaattcgtag atattcgtaa
      181 gaacaccagt ggcgaaggcg gctttctggc tcattactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006099
VERSION     KHUT01006099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttgga
       61 taagtttctt gtgaaagctc ccggcttaac tgggagaggc caaggaatac tatctgacta
      121 gagtgtggta ggggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaggca ggttactggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006100
VERSION     KHUT01006100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcgc
       61 taagttggat gtgaaaactc tgggcttaac ccagagcctg cattcaaaac tgacgggcta
      121 gagttctgga ggggatagcg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagaaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006101
VERSION     KHUT01006101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           88 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggttcgta ggtggtttga
       61 taagttatct gtaaaagccc tgggctcaac ctaggaaggc cagataaaac tgttagactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006102
VERSION     KHUT01006102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacgtatggg gcaagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggcttgg
       61 taagtcagat gtgaaaactg ggggctcaac ccgcagcctg catctgaaac tgtcaggctt
      121 gagtctggta gaggaaagtg gaattcccag tgtagcggtg aaatgcgcag atattgggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006103
VERSION     KHUT01006103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgaagggg actagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggactct
       61 taagtcaggg gtgaaatgcc agggctcaac cttggaattg cctttgatac tgaaagtctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006104
VERSION     KHUT01006104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           38 c           88 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcacgta ggcggttatt
       61 taagttagat gtgaaatacc tgggcttaac cgaggaattg catctaagac tggatgactt
      121 gagtatagta gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gctatctgga ctgatactga cgctgaggtg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006105             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006105
VERSION     KHUT01006105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcat
       61 taagttgggt gtcaaaggca atggctcaac cattgtacgc acccaaaact ggtgaacttg
      121 agtgcaggag aggggagtgg aattcccggt gtagcggtgg aatgcgtaga gatcgggagg
      181 aacaccagtg gcgaaggcga ctctctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01006106             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006106
VERSION     KHUT01006106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcagg
       61 taagtctggt gtcaaagccc ggggctcaac cccggtacgc attggaaact gcctgacttg
      121 agtgcagaag aggaaagcgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg ctttctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01006107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006107
VERSION     KHUT01006107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           50 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ttgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc ctggcttaac cagggaattg cgcccaaaac tactgcgctg
      121 gaggaagaca gaggtaagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006108             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006108
VERSION     KHUT01006108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttttt
       61 taagtctggt gtataatcca cgggctcaac ccgtgttcgc accggaaact gggagactgg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01006109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006109
VERSION     KHUT01006109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c          102 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccgt
       61 caagtctcgg gtgaaagccc ccggctcaac cggggaagtg cctgggatac tggcgggctc
      121 gagggggata ggggcggacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gtccgctggg tccccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006110
VERSION     KHUT01006110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           87 g           61 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccgaaggt
       61 taagcgtgtt gtgaaatgta gtcgctcaac gggtgcactg cagcgcgaac tggctttctt
      121 gagtgcgcgg aaagttggcg gaattcgttg tgtagcggtg aaatgcttag atatgacgaa
      181 gaactccgat tgcgaaggca gctgactgta gcgtaactga cgctgatgct cgaaagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01006111             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006111
VERSION     KHUT01006111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttttt
       61 taagtccgat gtataagccc ggggctcaac cccgggaggc atgggaaact gggaggcttg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01006112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006112
VERSION     KHUT01006112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           37 c           76 g           64 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatga ctgggcgtaa agggtccgta ggcagcattt
       61 tttgttagag gttaaataaa aaagcttaac ttttttccgc ttctaaaacg taaatgcttt
      121 gagtttgata ggagatagca gaatttcaca tggaggggtg atatccgtag atatgtgaag
      181 gaataccaga ggcgaaggcg gctatctaga tcaatactga cgttgaggga cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006113
VERSION     KHUT01006113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcgggaatg
       61 caagtccggt gggaaagccc acggctcaac cgtggaagtt cgctggaaac tgcatttctt
      121 gagggcgata gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctgga tcgaccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006114
VERSION     KHUT01006114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           93 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggtgtgg
       61 caagtctgat gtgaaaggca tgggctcaac ctgtggactg cattggaaac tgtcatactt
      121 gagtgccgga ggggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cggtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006115
VERSION     KHUT01006115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agagcacgta ggtggtgcgc
       61 taagtcgtgt gtgaaatgcc ccggctcaac cggggaattg cgctcgatac tggcgtgcta
      121 gaggtcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg actcactgga ccgattctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006116
VERSION     KHUT01006116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgagaggt ccaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcgggcttg
       61 taagtgtctt gtgaaatccc cgggctcaac ctgggaattg cagggcatac tgcgagtcta
      121 gaggaatcta gaggccggca gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaatgccaaa ggtgaagaca gccggctggg gatttcctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006117
VERSION     KHUT01006117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           83 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggggcgta ggctggatag
       61 taagtcagac gtgaaatccc ggggcttaac ctcggaactg cgtttgaaac taccattctt
      121 gaggataggc ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttagacctga cgctgatgcc cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006118
VERSION     KHUT01006118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tggcgatctt
      121 gggccactta ggggctagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gctagctggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006119
VERSION     KHUT01006119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 aaagtcgcgc gtgaaaggct ccggctcaac cggagacggc cgtgcgaaac tttcgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cgacatctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006120
VERSION     KHUT01006120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagtcgggt gtgaaatccc ggggctcaac cccggaactg cactcgatac tgccttgctt
      121 gagcactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctaga cagttgctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006121
VERSION     KHUT01006121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           87 g           57 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggtttag
       61 taagttggaa gtgaaagccc ggggcttaac ctcggaattg ctttcaaaac tactaatcta
      121 gagtgtagta ggggatgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccggt ggcgaaggcg gtcatctggg ctacaactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006122
VERSION     KHUT01006122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agggcgtgca ggtggttccg
       61 taagtggtgc gtgaaagcgc tcggctcaac cgggcgaggc cgtgccaaac tgcggaacta
      121 gaaacagaca gaggcaagtg gaattcgggg tgtagtggtg aaatgcgtag agatcccgag
      181 gaactccagt ggcgaaggcg acttgctggg tctggtttga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006123
VERSION     KHUT01006123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          100 g           48 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgtcc gtgaaaacct ggggctcaac ttcaggtctg cggacgatac gggcaggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006124
VERSION     KHUT01006124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcttt
       61 caagtcgggg gtgaaatccc acagcttaac tgtggaagtg cctccgatac tgggagcctt
      121 gagggaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006125
VERSION     KHUT01006125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 ccagtcaggg gtgaaatcgt tcggctcaac cggacaagtg cccctgatac tgagagtctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006126
VERSION     KHUT01006126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agagcgtgta ggcggtttcg
       61 tatgtctgac gtgaaagccc cgggctcacc ccgggaagtg cgttggaaac tgcgagacta
      121 gagtctcgga gaggaaggtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006127
VERSION     KHUT01006127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgcag
       61 caagttgggg gtgaaatccc gaggctcaac ctcggaattg ccttcaaaac tgctgtgctc
      121 gagttcggaa gaggtgagtg gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccgcaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006128
VERSION     KHUT01006128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           96 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttcgc
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaattg cctgggatac tggcgagctc
      121 gagggaggta ggggccgcgg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg cgtggctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006129
VERSION     KHUT01006129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           83 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtac agggcgcgta ggcgggaagg
       61 aaagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac ttccattctt
      121 gaggataggc ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccggt ggcgaaagcg gttttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01006130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006130
VERSION     KHUT01006130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           94 g           48 t
ORIGIN      
        1 tacggggggt gcgagcgtta atcggaatta ctgggcgtaa agggcatgta ggcggtctgc
       61 taagttaggt gtaaaagccc tgggcttaac ctgggaacga catttaaaac tgacagacta
      121 gagtgatgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccggt ggcgaaggcg gccccctggg aatcaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006131
VERSION     KHUT01006131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           97 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggctcgt
       61 taagtctgtc gtgaaatctc ccggcttaac cgggataggt cgacggatac tggcgggctt
      121 gaggggagca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg ctctacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006132
VERSION     KHUT01006132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatta ttgggtttaa agggtgcgca ggcgggatgc
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cctttgatac tggtgtcctt
      121 gaatgtactt gaggtaggcg gaatgtggca agtagcggtg aaatgcatag atatgccaca
      181 gaacaccgat tgcgtaggca gcttactaag gtatgattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01006133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006133
VERSION     KHUT01006133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           90 g           44 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc accgctcacc ggtggaactg ctgggcatac tggcgatctt
      121 gagccatcta ggggcgactg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaag ggtgaaaaca ggtcgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006134
VERSION     KHUT01006134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           47 c           98 g           53 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgtc gtgaaagatc caggcttaac ctgggttctg cggtggatac gggcagactt
      121 gaggcaagta ggggagatcg gaattccacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gatctctggg cttgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006135
VERSION     KHUT01006135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtctgat gtgaaatctc tgagcttaac tcagaaactg catcggatac tattcagcta
      121 gaggattgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006136
VERSION     KHUT01006136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttga
       61 aaagtcagat gtgaaagccc cgggctcaac ttgggaactg catttgatac tgtcagacta
      121 gagtacagaa gaggtgactg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccaga tgcgaaggcg ggtcactggg ctgatactga cgctaaagcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006137
VERSION     KHUT01006137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           97 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggccatt
       61 caagtcagat gtgaaagccc cgggctcaac ctgggaggtg catttgggac tggatggctt
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006138
VERSION     KHUT01006138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 gacggatggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccact
       61 taagttagac gtgaaagccc taggcttaac ctaggaactg cgtttaatac tgggtggcta
      121 gagtatggta gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccaga ggcgaaggcg gctttctgga ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006139
VERSION     KHUT01006139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           77 g           57 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggagcgta ggcggacttt
       61 taagtcagtg gtgaaagctt cccgcttaac gggaaaactg ccattgaaac tgagagtctc
      121 gagtatggtt gaggttactg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccaat tgcgaaggca ggtaactaag ccataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006140
VERSION     KHUT01006140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          106 g           40 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggttcgg
       61 caagtccatg gtgaaagcgc ccggctcaac tgggcgagtg ccgtggggac tgccgagctg
      121 gaggggagca gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ctctacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006141
VERSION     KHUT01006141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           84 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggacctt
       61 taagtaggaa gtgaaaggta agagctcaac tcttacactg ctccctatac tgggggtctt
      121 gagtaccgga gaggacaagg gaacaggtcg tgtagcggtg aaatgcgttg agatgacttg
      181 gaacaccaat ggcgaaagca cttgtctgga cggagactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01006142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006142
VERSION     KHUT01006142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           81 g           64 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagcg gtgaaatatc tcagctcaac tgagagggtg ccattgatac tgatgagctt
      121 gagtgaagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccaat agtgaagaca gtttactaga cttttactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006143
VERSION     KHUT01006143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           90 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaccag
       61 caagtcaggc gtgaaatccc tgggctcaac ccaggaactg cacctgaaac tgctggtctg
      121 gagtggtgga gagggaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cactaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006144
VERSION     KHUT01006144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           96 g           45 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggcctgg
       61 caagtttccg gtgaaatctc ccggctcaac tgggaggggt ccgggaaaac tgccaggctt
      121 gagtcatgga gagggatgcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc catgtactga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006145
VERSION     KHUT01006145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          104 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgcgta ggtggcaggc
       61 caagtctgct gtgaaatcgc ccggctcaac tgggcgcagt cagtggaaac tggtcagctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006146
VERSION     KHUT01006146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 gacagggggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctcg
       61 taagtcgggt gtgaaagccc tcagcctaac tgaggaactg cgcccgatac tgcaaggcta
      121 gaggatggga ggggagagtg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaataccagt ggcgaaggcg actctctggt ccattcctga cgctgaggtg cgagagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006147
VERSION     KHUT01006147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggtggtttgg
       61 taagtctttt gtgaaagctc ccggcttaac tgggagaggt caagggaaac tgccggacta
      121 gagtttggta ggggatactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctat ggcgaaagca ggtatctggg ccataactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006148
VERSION     KHUT01006148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           51 t
ORIGIN      
        1 tacatactgt gcgaacgtta ttcggattca ctgggcttaa agggtgcgta ggcggcttgt
       61 taagtagggt gtgaaagccc ccagctcaac tggggaactg cactctatac tggcgagctg
      121 gagtgagata ggggtgtgtg gaactgccag tggagcggtg aaatgtgttg atattggcag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006149
VERSION     KHUT01006149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           82 g           58 t
ORIGIN      
        1 gacggggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtgtgca ggtggttttg
       61 taagttagat gtgaaagcct tgggcttaac ccaagaagtg catttaaaac tacaaaactt
      121 gagtatggaa gagggtcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgacctggt cctatactga cactcataca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006150
VERSION     KHUT01006150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           83 g           51 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgcgcag
       61 aaagttgggt gtgaaatacc tcggcttaac cgaggaactg cgcccaaaac tactgtgctt
      121 gaggaagata gaggtgagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacacctgt ggcgaaagcg gctcactggg tcttttctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006151
VERSION     KHUT01006151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctatt
       61 caagtcaggg gtgaaagccc ggggctcaac cctggaactg cctttgaaac tagatagctg
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006152
VERSION     KHUT01006152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c          101 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggtcctg
       61 gacgtgccgt gtgacagacg tcggctcaac cgacgtaggc cagggtacac gacaagactg
      121 gagacagaga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcatcctggc tctgttctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006153
VERSION     KHUT01006153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggactgg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgctggtctt
      121 gagccatcta ggggttgccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggcaactggg gatgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006154
VERSION     KHUT01006154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           89 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggtggacttt
       61 caagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgttggtctt
      121 gagtccggaa gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gctcactggt ccggtactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006155
VERSION     KHUT01006155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           85 g           50 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgcgca ggcggtgcgc
       61 taagtcaact gttaaatctc ccagcctaac tgggaatcag cggtagatac tggcgtgcta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006156
VERSION     KHUT01006156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttga
       61 taagtcctgt gtgaaagccc tgggctcaac ccaggaagtg cgcaggatac tgttgagctg
      121 gagtagcgga gaggatggcg gaatacccgg tgtagaggtg aaattcgtag atatcgggtg
      181 gaacacctgt ggcgaaggcg gccatctgga cgttcactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006157
VERSION     KHUT01006157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggttgttgcg
       61 taagttagtt gtgaaatccc cgggcttaac ctgggaactg caactgatac tgcgtgacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006158
VERSION     KHUT01006158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgggt gtgaaagctt ggggcttaac cccaagtctg catccgatac gggcagacta
      121 gaggcaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006159
VERSION     KHUT01006159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccaac
       61 caagtcagtg gtgaaatcct gcagcttaac tgtagaagtg ccattgatac tggtcggctc
      121 gagggaggta ggggtatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006160
VERSION     KHUT01006160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           84 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggggtg
       61 ccagtcaatt gtgaaatccc ggggctcaac cccggaactg cagttgaaac tacatcccta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01006161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006161
VERSION     KHUT01006161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttag
       61 taagtcagac gtgaaagccc tcggcttaac cgaggaagtg catctgatac tgctttactt
      121 gagtgtcgga ggggatggta gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaatacctgt ggcgaaggcg accatctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006162
VERSION     KHUT01006162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggtggtcttg
       61 tgtgtctggt gtgaaagccc ttggctcaac caaggaagtg cgccggaaac tgcaagactc
      121 gagtttggga gaggatggta gaattctcag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccggt ggcgaaggcg accatctgga ccaacactga cactgaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006163
VERSION     KHUT01006163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cccctgatac tgagagtctc
      121 gagggaggta ggggcatgtg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006164
VERSION     KHUT01006164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           85 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggaggtttgg
       61 taagtctgtt gtaaaagctc ccggcttaac tgggagaggc caacggaaac tgccaggctt
      121 gagtgtggta gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggctactgga ccatcactga ctctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006165
VERSION     KHUT01006165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaatac tgccgtgcta
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006166
VERSION     KHUT01006166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           87 g           46 t
ORIGIN      
        1 tacagacggt ccaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggtccag
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac tactggactt
      121 gaggaggcta gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gcttactggg acctttctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006167
VERSION     KHUT01006167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           89 g           56 t
ORIGIN      
        1 tacgtatggg gcgagcgttg ttcggaatta ttgggcgtaa agggagcgta ggcgggatat
       61 taagtcaggt gtgaaagtcc acggctcaac tgtgggattg catttgaaac tgatattctc
      121 gagtttggga gagggagacg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gtctcctggc ctaaaactga cgctgatgct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006168
VERSION     KHUT01006168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           87 g           47 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtgtgg
       61 taagtctgtt gtggaagctc ccggcttaac cgggagaggt caacagaaac taccacgctt
      121 gagtgtagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ctattactga cactaagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006169
VERSION     KHUT01006169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccatg
       61 tgtgtcggat gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tgcatggctt
      121 gagtacggga gagggttgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcagcctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006170
VERSION     KHUT01006170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           94 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccga
       61 caagtccgcc gtgaaagtcc gaggctcaac ctcggaatgc cggtggaaac tgtcgggctt
      121 gagttcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006171
VERSION     KHUT01006171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatcct ccggctcaac cggagaagtg cttctgatac tggcgggctg
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006172
VERSION     KHUT01006172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggctttt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tggggagcta
      121 gagtatgtga gaggggggta gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaataccagt ggcgaaggcg gccccctggc acaatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006173
VERSION     KHUT01006173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           98 g           42 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatga ctgggcgtaa agggagcgca ggcggtccgt
       61 taggtccgga gtgaaagccc ggggctcaac cccggagggt cttcggaaac cggcggactc
      121 gagtacggaa gaggagagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccgatactga cgctcaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006174
VERSION     KHUT01006174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           90 g           55 t
ORIGIN      
        1 tacggggggt gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtttta
       61 taagtcagat gtgaaatacc tgggcttaac cgaggaagtg catttgaaac tatgagactt
      121 gagtatggga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctacctgga ccaatactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006175
VERSION     KHUT01006175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           82 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccaat
       61 taagtcagtg gtgaaatcct tccgcttaac gggagaactg ccattgaaac tggttggctt
      121 gagtacggtt gaagtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat agcgtaggca gcttactaag ccgtaactga cgctgaggca cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01006176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006176
VERSION     KHUT01006176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggctggg
       61 caagtttgat gtgaaagctc cgggctcaac ccggaaaatg cattgaaaac tgcccggctt
      121 gaggatcgga gaggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ggtctctgga cgattcctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01006177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006177
VERSION     KHUT01006177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           98 g           44 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcacca
       61 caagtatact ctgaaatctc cctgctcaac ggggagggtg gagtatagac tgtggagcta
      121 gagggcggta gagggtggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccacctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006178
VERSION     KHUT01006178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           97 g           44 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagtctggc gtgaaagctc tcggctaaac cgggagaggt cgttggatac tgatggacta
      121 gaggacggta gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg actcgctggg ccgtacctga cgctaagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006179
VERSION     KHUT01006179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           86 g           47 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagttgggt gtgaaatctc tcggctcaac cgagaaattg cgcccaaaac taccgtgctc
      121 gagggagaca gaggtgagtg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaagcg actcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006180
VERSION     KHUT01006180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           85 g           52 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cccttgatac tgtcgggctt
      121 gaatgtacat gaggtaggcg gaatgtggca agtagcggtg aaatgcatag atatgccaca
      181 gaacaccgat tgcgaaggca gcttaccaag gtatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006181
VERSION     KHUT01006181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          102 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggggagg
       61 aaagtccgct gtgaaagccc gaggctcaac ctcggcgggt cggtggatac ttcctctctt
      121 gaaggtgcta gggggaaatg gaatgaccgg tgtagcggtg aaatgcgtag atatcggtcg
      181 gaacaccagt ggcgaaggcg gtttcctggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006182
VERSION     KHUT01006182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           47 c           81 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgca ggcggggatg
       61 caagtcagat gtgaaatccc ggggctcaac ctcggaacag catttgaaac tacatctcta
      121 gaggatagac ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagt ttatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01006183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006183
VERSION     KHUT01006183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           98 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcatgg
       61 taaggctgac gtgaaagctc ctggcttaac tgggagaggt cgttggcgac tgccaggctt
      121 gaggtgagga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgcaagcg gcgccctagc cttgacctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006184
VERSION     KHUT01006184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           74 g           66 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaatat
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaactg tcattgatac tggtattctt
      121 gaatatagtt gaggtgggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctatcattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006185
VERSION     KHUT01006185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           90 g           45 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaactg cgctctaaac taccgtgctc
      121 gagggaggta gaggtgagcg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaagcg gctcactgga cctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006186
VERSION     KHUT01006186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           89 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatga ctgggcgtaa agggagcgta ggtggccttt
       61 taagtgaaat gtgaaagccc ggggctcaac tccggaatgt catttcatac tggaaggctg
      121 gagtgcagga gaggtaagcg gaattcctag tgtagcggta aaatgcgtag atattaggaa
      181 gaacaccaga ggcgaaggcg gcttgctgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006187
VERSION     KHUT01006187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           40 c           91 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgaa
       61 taagttatct gtgaaattcc tgggctcaac ctggggtggt cagatgatac tgtttaactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006188
VERSION     KHUT01006188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           90 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtgatg
       61 tatgtctgag gtgaaagccc caagcttaac ttgggaagtg ccttggaaac ggcatcacta
      121 gagtgctgga gaggttcgta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccaga ggcgaaggcg gcgaactgga cagcaactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006189
VERSION     KHUT01006189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           75 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattgt
       61 taagtcagag gtgaaatttt gcggctcaac cgtaagcttg cctttgatac tgatgatctt
      121 gaattaactt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaag gttttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006190
VERSION     KHUT01006190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgtgta ggcggcgccg
       61 taagtcaggt gtgaaatctc ggggcttaac tccgaaactg catttgatac tgcggtgctt
      121 gaggactgga gaggagactg gaatttacgg tgtagcggtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cgctgagaca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006191
VERSION     KHUT01006191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           86 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaga
       61 taagttaggt gtgaaagccc cgggcttaac ctgggaattg cgcctaatac tgtttgactt
      121 gagtactgta gaggaaggtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatctgt ggcgaaggcg accttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006192
VERSION     KHUT01006192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          101 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 ccagtcaacg gtgaaagctc gacgctcaac gtcgagtcgg ccgtcgatac tgccgcgctg
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006193
VERSION     KHUT01006193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgctg
       61 taagtcgggt gtgaaatcct gaggcttaac ctcagaactg cattcgatac tgcggcgctt
      121 gaggactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag agatcatgag
      181 gaagaccagt ggcgaaggcg ggtctctgga caggtcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006194
VERSION     KHUT01006194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcccct
       61 taagtccgtg gtgaaagcct gccgcttaac ggcagaactg ccatggatac tgaggggctt
      121 gagtccagac gaggttggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006195
VERSION     KHUT01006195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           81 g           55 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggatgcc
       61 taagtcagtg gtgaaagccg gcagcttaac tgtcgaattg ccattgaaac tgggcgtctc
      121 gagtgcggtt gaggtgactg gaatgtaaca tgtagcggtg aaatgcatag atatgttaca
      181 gaacaccgat tgcgaaggca ggtcactaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006196
VERSION     KHUT01006196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           88 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatca ctgggcgtaa agggtgcgta ggtggttgaa
       61 taagtcagat gtgaaatccc tgggctcaac ttaggaattg catttggtac tgttcagcta
      121 gagtgcagga gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006197
VERSION     KHUT01006197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           82 g           56 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactgg
       61 ttagtcaggg gtgaaatccc gtcgcttaac gacggacctg cctttgatac tgccagtctt
      121 gaattaggga gaggttcgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcgaactggc cctatattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01006198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006198
VERSION     KHUT01006198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcgata
       61 caagtcagag gtgaaagcct ggagctcaac tccagaattg cctttgaaac tgtattgctt
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006199
VERSION     KHUT01006199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcagat
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggaaac tggtgtgctg
      121 gagggaggta ggggtcgagg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006200
VERSION     KHUT01006200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcggg
       61 taagtcacct gtgaaatctc ctggctcaac tgggagtctg caggcgaaac tgcttgactg
      121 gagtatggga gaggtgcgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006201
VERSION     KHUT01006201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtctagg gtgaaatccc agggcttaac cctggaagtg ccttagaaac ggtaagacta
      121 gagtcttaga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga agcgaaggcg gcgacctgga taagaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006202
VERSION     KHUT01006202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccag
       61 ccagtctgtg gtgaaatccc ccggcttaac cggggaagtg ccgtggatac tgctggcctc
      121 gaggagggta ggggcatctg gaacggtcgg tggagcggtg aaatgcgttg atatcgaccg
      181 gaactccggt ggcgaaggcg aggtgctggg ccctttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006203
VERSION     KHUT01006203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggctggg
       61 taggtccgct gtgaaaactc gaggctcaac ctcgagacgt cggcggaaac catccagcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006204
VERSION     KHUT01006204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           97 g           47 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgcgt
       61 taagttaggc gtgaaagccc tgggctcaac ccgggaactg cgcttgatac tggcgcgctt
      121 gaatccagga gaggttggtg gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ctggtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006205
VERSION     KHUT01006205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           85 g           51 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatga ttgggcgtac agggtgcgca ggcggcatgg
       61 taagtcagct gttaaaaacc tcagcctaac tggggatcgg cggcagatac tgctatgcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006206
VERSION     KHUT01006206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           55 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtaagag gtgaaagcct ggagctcaac tccagaattg ccttttagac tgcatcgctt
      121 gaatccaaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ttggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006207
VERSION     KHUT01006207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           54 c          106 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttcat
       61 taagtctggt gtgaaagctc ccggctcacc tgggagaggg cactggagac tggtggactg
      121 gagcgaggca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ccttgactga cgctgaggcg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006208
VERSION     KHUT01006208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgagg
       61 caagtcggat gtgaaatccc ggagctcaac tccggaactg cattcgatac tgctttgctc
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006209
VERSION     KHUT01006209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          100 g           43 t
ORIGIN      
        1 tacgtacgag gcgagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggtggctcgt
       61 taagtagggc gtgaaatctc ccggcctaac tgggaggggt cgccctagac tggcggactt
      121 gaggactgca gaggtgggtg gaattcctgg tgtagtggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcccactggg cagtcactga cgctgaggcg cgaaagctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006210
VERSION     KHUT01006210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           89 g           45 t
ORIGIN      
        1 taccagctcc tcgagtggtc ggggcgatta ttgggcctaa agcatccgta gccggttccg
       61 taggtcctct gttaaatcca acggcttaac cgttggcccg cagaggatac cgcgggacta
      121 ggaggcggga gaggcaggcg gtactctacg tgtaggggta aaatcctttg atccgtagaa
      181 gaccaccagt ggcgaaggcg gcctgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006211
VERSION     KHUT01006211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcacgca ggcggtccat
       61 taagttcagg gtgacagtca tcagcttaac tgatgaagtg ccttgaaaac tggtggactt
      121 gagagcgaga gagggacacg gaactccggg tggagcagtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtttctga cgctcaggtg cgaaagcgag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006212
VERSION     KHUT01006212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttct
       61 taagtgaggg gtgaaaggtt tgggctcaac ccaaacactg cctttcatac tgggagcctt
      121 gagtatggga gaggcgagtg gaatggtcgg tgtagcggtg aaatgcgtag atatcgatcg
      181 gaacacccat ggcgaaggca gctcgctggc ctaaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006213
VERSION     KHUT01006213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggttcgt
       61 cgcgtcgatc gtgaaaactt gcagctcaac tgcgagcttg cggtcgatac gggcggacta
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgagaag cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006214
VERSION     KHUT01006214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtccgtg gtgaaatctc tgggcttaac ccagaaactg ccatggatac tatttgtctt
      121 gaatgttgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006215
VERSION     KHUT01006215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggctctc
       61 aaagtcaggg gtgaaatccg gcagcttaac tgtcgaagtg cctttgatac tggggagctc
      121 gagggaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006216
VERSION     KHUT01006216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggctatt
       61 cgcgtcgggt gttaaagctc agggctcaac tctgagaggc cattcgatac gggatagcta
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cactgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006217
VERSION     KHUT01006217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          103 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcctgta ggcggcctgt
       61 caagtccggc ctgaaagccc cccgctcaac gggggagggt ggctggaaac tggcaggctt
      121 gagggcgaga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccctctggc tcgaccctga cgctgagagg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006218
VERSION     KHUT01006218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcctt
       61 caagtccggg gtgaaatcct ctcgcttaac gagagaactg ccccggatac tggaggcctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006219
VERSION     KHUT01006219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggctgt
       61 taagtggtgc atgaaatctc cgcgcttaac gtggagaggc tgtgccagac tggcagtcta
      121 gagtacgaga gagggttgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcaacctggc tcgtaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006220
VERSION     KHUT01006220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ctgggcgtaa agcgcgttaa ggcggttggc
       61 aaagttggtt gtgaaatctc agggctcaac cctgaaattg cgaccaatac tggtcgacta
      121 gagtgtgaga gaggaaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggc tcataactga cgctgtagcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006221
VERSION     KHUT01006221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           85 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggatgta
       61 taagtcagat gtgaaattcc gaggctcaac ttcggagctg catttgaaac tatagatctc
      121 gaggattggc ggagagaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaagcg gttctctagc taaaacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01006222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006222
VERSION     KHUT01006222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           88 g           50 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcggattcg
       61 caagtaggat gtgaaagccc caggcttaac ctgggaatgg caccctaaac tacggatctt
      121 gaatgcttaa gaggttggtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaatatcgga ggcgaaggcg gccaactggt gatgcattga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006223
VERSION     KHUT01006223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 tacatactgt gcgaacgtta ttcggattta ctgggcttaa agggtgcgta ggcggttcgt
       61 taagtagggc gtgaaagccc ctggctcaac cagggaattg cactctaaac tggcgggcta
      121 gagtgagata ggggtgtacg gaactgccgg tggagcggtg aaatgtgttg atatcggcag
      181 gaacgccggt ggcgaaagcg gtacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006224
VERSION     KHUT01006224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           97 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg atcggaatta ctgggcgtaa agggcgcgta ggcggttaca
       61 tgagtgtgct gtgaaagccc cgggcttaac ctgggaagtg cagtgcaaac gatgtaactg
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006225
VERSION     KHUT01006225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          102 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcgggt
       61 cacgtcgggt gtgaaagccc ggggcttaac cccgggtctg cattcgatac gggcttgcta
      121 gggtccggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccggtaccga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006226
VERSION     KHUT01006226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggttgtctt
      121 gggccatcta ggggctactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca agtagctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006227
VERSION     KHUT01006227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgcc
       61 taagtcggac gtgaaatccc tcggctcaac tgaggaactg cgtccgagac tgggtggctg
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006228
VERSION     KHUT01006228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatca ctgggcgtaa agggtccgta ggcggaacga
       61 caagtcggac gtgacaaggc gcggctcacc cgcgcgaggt cgttcgaaac tgtcgctcta
      121 gaatcggtgc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggaa
      181 gaacctcaac ggcgcaggca gccggctagt gccagattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006229
VERSION     KHUT01006229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ctgggtgtaa agggtgcgta ggcgggacaa
       61 taagtcagtg gtgaaatctt acggctcaac cgtgaaactg ccattgatac tgttgttctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccgaaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006230
VERSION     KHUT01006230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c          103 g           44 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggtgggt
       61 taagtctgtt gtgaaagcgc ccggctcaac cgggcagagt caatggaaac tagctcactg
      121 gaggtcggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006231
VERSION     KHUT01006231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           90 g           52 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggattca ctgggcttaa agggcgcgta ggctgtttac
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggtgaactc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctggc cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006232
VERSION     KHUT01006232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           87 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcttgg
       61 taagtcagga gtgaaagttt gcggctcaac cgtaaaattg cttttgatac tgccgggctg
      121 gaattaggat gaggtcagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacacctat tgcgaaggca gctggctaga cctatattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006233
VERSION     KHUT01006233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggc
       61 caagtcaggg gtgaaatcct gcagcttaac tgtagaagtg cctttgatac tggccggctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acagactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006234
VERSION     KHUT01006234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           94 g           40 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggaccgg
       61 aacgcgtccc gtgaaatccc ccggctcaac cggggaactg cggggcgaac ggacggtctt
      121 gaggcataca tgggcgggcg gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg gcccgctggg tatgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006235
VERSION     KHUT01006235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggatagg
       61 taagtccgtg gtgaaatctc ccgacttaat cgggaaactg ccgtggatac tatctatctt
      121 gaatgccgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctaactacg cggtgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006236
VERSION     KHUT01006236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agagaacgta ggcggtttgc
       61 caagtttgat cttaaagacc ggggcttaac tccgggagtg gatcgaaaac tggcagacta
      121 gaggacgtca gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg actttctggg acgttcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01006237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006237
VERSION     KHUT01006237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcaggta ggtggtctta
       61 taagtctgct gtgaaatccc agggcttaac cctggaagtg cggcagatac tgcaagacta
      121 gagtgctgga ggggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgcactgga cagcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006238
VERSION     KHUT01006238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           83 g           58 t
ORIGIN      
        1 tacgtagggt gctagcgtta ttcggaatta ctgggcgtat agggtgcgta gacggcttat
       61 taagtcagtt gttaaaaatt ccggcctaac cggaaaagtg cggttgatac tgatgagcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggtc aaatgcgtag atatcgagag
      181 gaacatcaat ggcgaaggca gcttcttggt ccattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006239
VERSION     KHUT01006239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 cacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca gactgactag
       61 caagtggtgt atgaaatcgc ggtgcttaac acagcgaggt tatgccagac tgctggacta
      121 gagtgcgaga gagggatgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaagcg gcgtcctggc tcgcaactga cgttcagaca cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006240
VERSION     KHUT01006240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattta
       61 taagtcagtg gtgaaagccc cgagctcaac ttgggaattg ccattgatac tgtagatctt
      121 gagtgcggtt gaggtaggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca gcttaccaag ccgatactga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006241
VERSION     KHUT01006241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatact
       61 taagtcgggg gtgaaagccc agggctcaac cctggaacag ccttcgatac tgggtatctt
      121 gagtacggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006242
VERSION     KHUT01006242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatatt
       61 taagtcagtg gtgaaatctc ggggctcaac cccgaaactg ccattgatac tatttatctt
      121 gaatactgtg gaggtgagcg gaatgtgtca tgtagcggtg aaatgcatag agatgacaca
      181 gaacaccgat tgcgaaggca gctcactacg cagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006243
VERSION     KHUT01006243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggaagcc
       61 taagtccgat ctgaaagccc ctggctcaac cagggatccg gaccggaaac tgggcttctt
      121 gaggacggca ggggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gcactctgga ccggacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006244
VERSION     KHUT01006244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggttagt
       61 tacgtcggga gtgaaaactc ggagcttaac tccgagcctg cttccgatac gggctgacta
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006245
VERSION     KHUT01006245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           93 g           44 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtcgaa
       61 caagtcgggc atgaaatcct tcggctcaac cgggggaggc tgtccgatac tgtccgactg
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaagcg gccacctgga ctgtacctga cgctgacacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006246
VERSION     KHUT01006246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           37 c           79 g           60 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtttgta ggcggttata
       61 caagtcagat gtgaaagccc aaggctcaac cttggaattg catttgaaac tgtatagcta
      121 gagaatgtta gaggtaagtg gaattcctag tgtaggagtg aaatccgtag agattaggaa
      181 gaatatcgat ggcgaaggca gcttactggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006247
VERSION     KHUT01006247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           85 g           46 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggaatta ctgggcgtaa agagtctgta ggcgtcccga
       61 caagttctcg cttaaatccc agggctcaac cttggagctg gcgggaaaac tttcgggata
      121 gaggacggca gaggcaagtg gaattaccgg tgtagcagtg aaatgcgtag atatcggtag
      181 gaacaccaat ggcgaaggca gcttgctggg ccgcccctga cgctgagaga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006248
VERSION     KHUT01006248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           87 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcaagca ggcggatcgt
       61 taagtagaaa gtgaaaggtc ggggctcaac cccaacattg ctttctatac tggcggtctt
      121 gagtcccgga ggggagagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctctctgga cgggtactga cgctcatttg cgaaagccga
      241 ggtagcgaac agg
//
LOCUS       KHUT01006249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006249
VERSION     KHUT01006249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c           99 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggtgggttcg
       61 ccagtctgtg gtgaaagccc ccagcttaac tggggaagtg ccgtggatac tgcgaacctc
      121 gaggagggta ggggcgtctg gaacggtcgg tggagcggtg aaatgcgttg atatcgaccg
      181 gaactccggt ggcgaaggcg aggcgctgga ccctttctga cactgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006250
VERSION     KHUT01006250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           96 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggat gtgaaatccc ggggctcaac cccgggtctg cattcgatac gggctgacta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006251
VERSION     KHUT01006251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacggagggc gcaagcgtta atcggaatca ctgggcgtaa agcgtgcgta ggctgtttga
       61 taagtcaggc gtgaaatccc tcggctcaac cggggaactg cgtttgatac tgttgaacta
      121 gagtcctgga gagggtggcg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga caggtactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006252
VERSION     KHUT01006252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacaat
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tggttgtctt
      121 gagccactta ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctcgctggg agtgtgctga cgctgagaca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006253
VERSION     KHUT01006253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggtggcacgg
       61 taagtcgatt gtgaaagccc ttggctcaac caaggaagtg caatcgaaac tgccgagctt
      121 gagtcctgaa gagggtggta gaatggccag tgtagaggtg aaattcgtag atattggcta
      181 gaataccagt ggcgaaggcg accacctggg aaggtactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006254
VERSION     KHUT01006254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccgag
       61 taagtccggt gtgaaaactc gaggcttaac cttgagatgt catcggaaac tattcggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006255
VERSION     KHUT01006255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           89 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggacttg
       61 taagtcagat gtgaaagccc tgggctcaac ccaggaactg catttgaaac tgcgagccta
      121 gagtgatgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga catcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006256
VERSION     KHUT01006256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           96 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgaa
       61 tgggtgtgat gtgaaagcct tgggcttaac ctgagaagtg catcgcaaac tgtttaactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006257
VERSION     KHUT01006257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          104 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcggga
       61 ccagcgtgcg gtgaaagctc ggggctcaac cctgagtcgg ccgtgcgaac tgtcttgctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006258
VERSION     KHUT01006258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcgagt gtgaaagccc caggcttaac ctgggaactg cgctcgatac tgttgagcta
      121 gagtgtggaa ggggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccaacactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006259
VERSION     KHUT01006259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           91 g           44 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggtgtaa agcgcgcgca ggcggtctcg
       61 aaagtccacg gtgaaagctc ccggcccaac tgggagaggc ccgtggaaac ttcgagactt
      121 gaaggcggga gaggcaaatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaggcg atttgctggc ccgcacttga cgctcaggcg cgaaagctag
      241 ggtagcgacc ggg
//
LOCUS       KHUT01006260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006260
VERSION     KHUT01006260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 tacgaaggtc ccgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgcgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccgtgcta
      121 gaggactgga gaggcaactg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagag
      181 gaacactcgt ggcgaaagcg agttgctgga cagtatctga cgctgaggct cgaaggccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01006261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006261
VERSION     KHUT01006261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           85 g           51 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctatt
       61 caagtcagag gtgaaagccc agggctcaac cttggaactg cctttgaaac tagatagcta
      121 gaattctgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006262
VERSION     KHUT01006262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggtggtctgt
       61 caagtcgggg gtgaaatctt tcggctcaac cggaaaattg ccttcgatac tgacaggctc
      121 gagggaggta ggggttgcgg gaactaccag tggagcggtg aaatgcgttg atattggtag
      181 gaacgccggt ggcgaaagcg cgcaactgga tctcttctga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006263
VERSION     KHUT01006263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           85 g           60 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtatgta ggcggctatt
       61 caagtcagtt gtgaaagccc cgggcttaac ccgggaagtg cttctgatac tgtttagctt
      121 gagtactaga gaggatagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctatctggc tatgtactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006264
VERSION     KHUT01006264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           80 g           59 t
ORIGIN      
        1 tacgaagggt ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtg gtgaaagact tcagcttaac tgaagaactg ccattgatac tgatgagctt
      121 gagtacagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006265
VERSION     KHUT01006265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggccggg
       61 aaagtctgat gtgaaatctc cgagcttaac ttggaaacgg cattggaaac tatctggctt
      121 gagggccgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg agtctctgga cggcctctga cactgaagca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006266
VERSION     KHUT01006266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c          102 g           35 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggtgcgg
       61 tcagtggggt gtgaaagcgg tcggctcaac cgaccgaggc catctcagac tgccgcactc
      121 gagcgctgca gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccgag ggggaagcca gccacctggg cagccgctga cgctgaggtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01006267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006267
VERSION     KHUT01006267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           83 g           58 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtcttgc
       61 caagtcttcg gtgaaatctc cgggcttaac tcggaaattg tcggggaaac tggcaggatt
      121 gagtatggta gaggcagatg gaattgaagg tgtagcggta aaatgcgttg atatcttcaa
      181 gaacaccagt ggcgaaggcg atctgctggg ccattactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006268
VERSION     KHUT01006268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           88 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggcgagg
       61 caagtcggac gtgaaatccc ggggcttaac cccggaactg cgttcgatac tgccttgctc
      121 gaggactgga gaggagactg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacgccaat ggcgaaggca agtctctgga cagttcctga cgctcatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006269
VERSION     KHUT01006269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtagg
       61 taagtccgtg gtgaaatctc caagcttaac ttggaaactg ccgtggatac tatctatctt
      121 gaaggacgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacc cgtttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006270
VERSION     KHUT01006270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           87 g           46 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggttcgg
       61 taagttcgat gtgaaagccc cgggcctaac ccgggaacgg cattgaaaac tactggactg
      121 gaggattgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cactgaggca cgaaagccaa
      241 gggagcaaac agg
//
LOCUS       KHUT01006271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006271
VERSION     KHUT01006271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           93 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacggg
       61 tcagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tgcccgtctt
      121 gagccactca ggggtcgtcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca gacgactggg ggtgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006272
VERSION     KHUT01006272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           85 g           50 t
ORIGIN      
        1 tacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatcgg
       61 taagtacctt gtgaaatccc acgactcaat cgtggaactg cttggtatac tgccagtctt
      121 gaggtactca ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaagaca gctcgctggg ggtattctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006273
VERSION     KHUT01006273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatgat
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cccttgatac tggccatctc
      121 gagtccggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006274
VERSION     KHUT01006274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taggtcgatt gtgaaagccc agggctcaac cttggaattg cagttgaaac cgcatagctt
      121 gaatgttgga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgcctggc caaacattga cgctaaggcg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01006275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006275
VERSION     KHUT01006275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          106 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggaccgc
       61 gacgtgccgt gtgacaagcc accgcttacc ggtggagggc catggtacac gagcggtctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggt cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01006276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006276
VERSION     KHUT01006276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttatct gtaaaagccc tgggcttaac ctgggaaggc cagataaaac tgttgaacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006277
VERSION     KHUT01006277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           36 c           78 g           67 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttta
       61 taagtcagtg tttaaagtct gcagcttaac tgtaggaatg gcattgatac tgtagaactt
      121 gagtaaagtt gaggtaggcg gaattgaagg tgtagcggtg aaatgcttag atatcttcaa
      181 gaacaccaat tgcgaaggca gcttactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006278
VERSION     KHUT01006278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           81 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggacggg
       61 taagtaggaa gtgaaaggtt ggagctcaac tccaacactg ctccctatac tgcctgtctt
      121 gaatgtcgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cgaatattga cgctcatgca cgaaagctaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01006279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006279
VERSION     KHUT01006279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccatt
       61 caggtcggtt gtgaaaactg gaggcttaac ctccagacgt cgaccgaaac cgtgtggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctgt tgcgaaggcg gctctctggt acggaactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006280             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006280
VERSION     KHUT01006280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           99 g           37 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacca
       61 gcaagtcggg agtgaaatct cggagctcaa ctccgcgact gcttccgaaa ctgcggacct
      121 ggagaccggt agaggtgggt ggaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacacctg tggcgaaggc ggcccactgg gccggatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006281             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006281
VERSION     KHUT01006281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           51 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac tgctatggct
      121 tgagttcggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggtctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006282             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006282
VERSION     KHUT01006282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtttt
       61 taagtcggat gtgaaatctc cacggctcaa ccgggaaacc gcatccgaaa ctggaaacct
      121 tgaggtatgg agagggaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacccg tggcgaaggc ggcttcctgg ccatatcctg acgctgaggt gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006283             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006283
VERSION     KHUT01006283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           97 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggaaat
       61 taagtgagaa gtgaaaggct ggggctcaac ctcatgcagt gcttctcata ctggttttct
      121 tgagggcggt agagaaaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggctttttgg gccgttcctg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006284             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006284
VERSION     KHUT01006284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           93 g           46 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggcttag
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgatac tgctatagct
      121 tgagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggtactg acactgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006285             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006285
VERSION     KHUT01006285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           80 g           54 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgca gatggtctga
       61 caagttagca gtggcaaatc gaggctcaac ctcgtggaaa ctggtaatac tgtctgactc
      121 gagtacagga gaggttaacg gaactccatg tggagcggta aaatgtgtag atatatggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01006286             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006286
VERSION     KHUT01006286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtgcgta ggcggaggct
       61 tgcgtgagat gcgaaatcct atggctcaac catatggact gtatctcata cggggcttct
      121 tgaggatggc agaggcaagt ggaattagcg gtgtagcagt gaaatgcgta gagatcgcta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatatctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006287             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006287
VERSION     KHUT01006287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           63 c           95 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggtggcttgg
       61 cacgtctctc gctgaaatcc cctggctcaa ccagggaagt ggcggggata cgaccaagct
      121 tgaggggggt aggggggacg ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg cggcgaaagc gcgtccctgg accccacctg acactgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006288             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006288
VERSION     KHUT01006288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           41 c           79 g           56 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta gacggtttgg
       61 taagttagca gtgggaaatc gaggcccaac ctcgtggaag ttgctaatac tactatactc
      121 gagtgcagga gaggttaacg gaactcaatg tggagcggta aaatgcgtag atatattgaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacgttgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01006289             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006289
VERSION     KHUT01006289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c          102 g           38 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggccgaa
       61 gaagtccatg ggtgaaaggc cggggctcaa ccccgtgcat gcccaggata ctgatcggct
      121 ggagacacgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006290             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006290
VERSION     KHUT01006290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           44 c           83 g           52 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatca ttgggcgtaa agggtgcgta gatggtctga
       61 taagttagcg gtgggaaatc gaggctcaac ctcgtggaag ccggtaatac tattcgacta
      121 gagggcagga gaggttaacg gaactccatg tggagcggta aaatgcacag atatatggaa
      181 gaacatcaat aacggcgaag gcagttaact attctgttcc tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01006291             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006291
VERSION     KHUT01006291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           88 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcccga
       61 taagtcagct gtgaaatcgc tgggctcaac ccatgcaatt gcagttgata ctgtcgggct
      121 tgaatggggt tgaggctggc ggaatgcgac atgtagcggt gaaatgctta gatatgtcgc
      181 agaacaccga ttgcgaaggc agccagctgg gcctttattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006292             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006292
VERSION     KHUT01006292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           83 g           56 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gacggtttgg
       61 taagttagca gtgggaaatc gaggcccaac ctcgtggaag ttgttaatac tgccatactc
      121 gagtgcagga aaggttgacg gaactcaatg tggagcggta aaatgcgtag atatattgaa
      181 gaacaccgat aacggcgaag gcagttaact gttctgttac tgacgttgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01006293             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006293
VERSION     KHUT01006293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           80 g           55 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggcctga
       61 caagttatca gtggcaaatc gaggctcaac ctcgtggaaa ctggtaatac tgttcggctt
      121 gagtacagga gaggttaacg gaactctatg tggagcggta aaatgcacag atatatagaa
      181 gaacatcaat aaaggcgaag gcagttaact attctgttac tgacgctgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01006294             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006294
VERSION     KHUT01006294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           45 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggacctg
       61 taagtttgac gtgaaatctc caccgctcac cggggagggg tcgttgaata ctgcaggtct
      121 ggagttcggc agaggaccac ggaattccgc gtgtagcggt ggaatgcgta gatatgcgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gcctgaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006295             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006295
VERSION     KHUT01006295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcggctttg
       61 taagttgaag gtgaaatacc agcggctcaa ccgtggaatg gccttcgaaa ctgcaatggc
      121 ttgagggagc taggggcatg tggaacttcc ggtggagcgg tgaaatgtgt tgatatcgga
      181 gggaacgcca gtggcgaaag cggcatgctg gagctttttc tgacgctgag gcgcgaaagc
      241 caggggagcg aacggg
//
LOCUS       KHUT01006296             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006296
VERSION     KHUT01006296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          106 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccgg
       61 caagtcggct cgtgaaagcc cggggctcaa ccccggaggt gcggccggga ctgtcgggct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006297             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006297
VERSION     KHUT01006297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           69 c          112 g           27 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggagtca ctgggcgtaa agggcgcgca ggcggccgcg
       61 ccggccatac cgtgaaagcc cccggctcaa ccgggcggat gcgggtggga ccgcgcggct
      121 ggagggtggg agaggcgggc ggaattcccg gtgtagtggt gaaatgcgta gataccggga
      181 ggaacacccg cggcgaaggc ggcccgctgg gccatccctg acgctgaggc gcgaaggcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006298             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006298
VERSION     KHUT01006298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttga
       61 taagtcgggt gtgaaacctc caggctcaac ctggagttgc cacccgatac tgttatggct
      121 tgagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006299             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006299
VERSION     KHUT01006299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcgggtcgt
       61 taagtcgggg gtgaaatcct gaggctcaac ctcagaactg ccttcgatac tggcgacctt
      121 ggagtccggg agaggtgagt ggaactccta gtgtagaggt gaaattcgta gatattagga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgatgt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006300             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006300
VERSION     KHUT01006300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           89 a           37 c           77 g           51 t
ORIGIN      
        1 tacggggggg gcgagcgtta ttcagaatga ctgggcgtaa agggtacgta ggcggtctat
       61 taagttgaag gtaaaagccc agagctcaac tctggaaaac ccttcaaaac tgatatgact
      121 agagtacgag agaggatagt agaatttcta gtgtagggat gaaatatgaa aagattagaa
      181 ggaataccaa aggcgaaggc aactatctag atcgatactg acgctgaggt acgaaagtat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006301             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006301
VERSION     KHUT01006301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatta ctgggcataa agcgcgcgta ggcggcgacg
       61 taagtgtagg gtgaaatccc acggcccacc cgtgtgaatt gccccgcata ctgcgttgct
      121 ggagtgtggt aggggagagc ggaactcctg gtggagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggctctctgg tccacaactg acgctgaagc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01006302             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006302
VERSION     KHUT01006302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          101 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgcgta ggcggttggt
       61 caagtcggac gtgaaagccc ctggctcaac tgggggaggt cgttcgatac tggtcggctt
      121 gagggtgaga gagggaagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gcttcctggc tcattcctga cgctgagagc gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006303             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006303
VERSION     KHUT01006303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           47 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgg
       61 taagttgggt gtgaaaactc caggctcaac ctggagacgc cacccgatac tgctgtgact
      121 tgagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01006304             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006304
VERSION     KHUT01006304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           65 c          104 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgc
       61 cgcgtcggtt gctgaaatcc cccggctcaa ccggggaacg ggcagcgata cgggcggcct
      121 ggaggggggt agggggggcc ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gggcccctgg acccttgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006305             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006305
VERSION     KHUT01006305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           91 g           55 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattca ttgggcgtaa agggagcgta ggcggtctgg
       61 taagtcgggt gtgaaatcct ggggctcaac cccagaactg cattcgatac tgctgggctt
      121 tgaggaatgg aggggagatt ggaattcacg gtgtagcagt gaaatgcgta gatatcgtga
      181 ggaagaccag tggcgaaggc ggatctctgg acatttcctg acgctgatgc tcgaaggcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006306             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006306
VERSION     KHUT01006306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttgtg
       61 gaagtcggat gtgaaatacc ggggctcaac tccggggcgc catccgatac tccactgact
      121 agagtccggt aggggaaggt ggaattcctg gtgtagcggt gaaatgcaca gatatcagga
      181 ggaacaccgg tggcgaaggc ggccttctgg gccggtactg acgctgagga gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006307             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006307
VERSION     KHUT01006307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           96 g           52 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcgtct
       61 taagtctgac gtgaaatctc ctggcttaac tgggaggggt cgttggaaac tgaggtgctt
      121 gaggcggtga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg acaccctggc actgtgcctg acgctcatgt gcgaaggcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006308             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006308
VERSION     KHUT01006308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           83 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatta ttgggcgtaa agagttgcgt aggcggacta
       61 gtaagttttt ggtgaaatcc ttcggcttaa ccggagaact gccaagaata ctactagact
      121 tagagggaat cagggggtgc tggaactcgt ggtgtagcag tcaaatgcgt tgatatcacg
      181 aggaacacca aaggcgaagg caggcacctg ggattcacct gacgctaagg cacgaaagcg
      241 tggggagcga aacgg
//
LOCUS       KHUT01006309             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006309
VERSION     KHUT01006309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          103 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtctt
       61 taaggctggt gtgaaaggtt cagggctcaa cccgaacagg gcactggcga ctggggacct
      121 tgagtggggg agagggaagc ggaattccgg gtgtagcggt gaaatgcgta gatatccgga
      181 ggaacaccga tggcgaaggc agcttcctgg cctcttaact gacgctgagg tgcgaaagcg
      241 tggggagcga acggg
//
LOCUS       KHUT01006310             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006310
VERSION     KHUT01006310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           98 g           49 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctat
       61 taagtgagaa gtgaaaggct ggggctcaac cccatgcagt gcttctcata ctggtagtct
      121 tgagggcggt agagagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccgttcctg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006311             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006311
VERSION     KHUT01006311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           55 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggtggcttga
       61 taagtctgat gtgaaagccc agggctcaac cctggaagtg cattggaaac tgtcgagctt
      121 gagtactgga gggggaagtg gaattctcga gtgtagaggt gaaattcgta gatattcgga
      181 agaacatcag tggcgaaggc gacttcctgg acagatactg acactgagac gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006312             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006312
VERSION     KHUT01006312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gtaagtctgg agtgaaatct cgctgctcaa cggcgagacg gcttcggata ctgcggatct
      121 ggagaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006313             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006313
VERSION     KHUT01006313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           96 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgc
       61 taagtcaggt gtgaaacctc caggctcaac ctggagccgc cacctgatac tggtatggct
      121 ggagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006314             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006314
VERSION     KHUT01006314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           82 g           58 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agagtgcgta ggtggtttaa
       61 taagttactg gtgggaaatc ggggctcaac cccgtggaag tcagtaatac tattagacta
      121 gagggccgga ggggttaacg gaattctatg tgtagcggta aaatgctcaa atatatagag
      181 gaacaccaat tggcgaaggc agttaactat tcggttcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01006315             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006315
VERSION     KHUT01006315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           91 g           51 t
ORIGIN      
        1 tacgaaggtt gcaagcgttg ttcggaatca ctgggcttaa agcgtatgta ggcggcccat
       61 caagtgcctt gtgaaatccc acggctcaac cgtggaattg cttggcatac tgttagggct
      121 tgaggcaggt aggggctcgt ggaactcttg gtggagcggt ggaatgcgta gatatcaaga
      181 ggaacgccgg tggcgaaagc ggcgagctgg gcctgtcctg acgctgagat acgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006316             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006316
VERSION     KHUT01006316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           66 c          100 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcgcgg
       61 agcatcgtcg gctgaaatcc cccggctcaa ccggggaacg ggccacgaga tgtccgcgct
      121 ggaggggggt agggggagcc ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gggctcctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006317             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006317
VERSION     KHUT01006317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           98 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacgt
       61 gcaagtcgga agtgaaatct cggggctcaa ctccgaggct gctcccgata ctgccgacct
      121 ggagaccagt agaggtgggt agaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaatacctg tggcgaaggc ggcccactgg gctggatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006318             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006318
VERSION     KHUT01006318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c          100 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatcgg
       61 cacgtccgtg gctgaaagcc cccggctcaa ccggggaagt ggcgcggata cggccgatct
      121 ggaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006319             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006319
VERSION     KHUT01006319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           50 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagacgc cacccgatac tgccgtgact
      121 tgagtctggt aggggagtgt ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtactg acactgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01006320             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006320
VERSION     KHUT01006320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          101 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccat
       61 gcagtcggca cgtgaaagcc cggggctcaa ccccggaacg gcggccgata ctcatgggct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006321             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006321
VERSION     KHUT01006321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          101 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagcgtgtg gtgaaagtcc ggggctcaac cccggatctg ccgtgcgaac tgccatggct
      121 agagtactgt agaggcaggt tgaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcctgctgg gcagttactg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006322
VERSION     KHUT01006322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           90 g           49 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggccgt
       61 taagtcaggg gtgaaagtcc gcggctcaac cgtgggaccg cccttgatac tggcggcctg
      121 gagtcgcggt gaagtggccg gaatatgtgg tgtagcggtg aaatgcatag atatcacatg
      181 gaataccgat tgcgaaggca ggccactaac cgcgtactga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006323
VERSION     KHUT01006323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggctagt
       61 aaagtcgagt gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tggctggcta
      121 gaagatggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggc ggcgaaggcg gccttctggg ccattcttga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006324             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006324
VERSION     KHUT01006324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           47 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggcggctcaa
       61 caagttgggt gtgaaacctc caggctcaac ctggagacgc cacccaaaac tgttgtggct
      121 agagtccggt aggggagcag ggaatatccg gtgtagcggt gaaatgctta gatatcgggt
      181 ggaacaccag tggcgaaggc gctgctctgg gccggaactg acgctgagga acgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006325
VERSION     KHUT01006325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           83 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatact tgagcttaac tgaaggcttg ccattgatac tgtagggctt
      121 gagtacagtt gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag ataccatcaa
      181 gaacaccgat tgcgaaggca gcttactaag ttgtaactga cgctgatgca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01006326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006326
VERSION     KHUT01006326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggccttg
       61 taagtcgggt gtgaaatccc acagctcaac tgtggaagtg cattcgaaac tgcgaagctt
      121 gagtctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag agattgggag
      181 gaacaccggt ggcgaaggcg accacctggt cgaagactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006327
VERSION     KHUT01006327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ttgggcgtaa agcgcgtgca ggcggcgata
       61 caagtctgat gtgaaagccc cgggcttaac ctgggaagtg cattggaaac tgtatggctt
      121 gagtactgga gaggaaggga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg cccttctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006328
VERSION     KHUT01006328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           77 g           56 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggcctgg
       61 taagtcagtg gtgaaatctc gcagcttaac tgcgaaactg ccattgatac tgctaggcta
      121 gagtatagat gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctataactga cgctgaggct cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006329
VERSION     KHUT01006329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtttga
       61 taagtccgct ctgaaagtcc ggggctcaac cccggaatgc gggtggaaac tgtcagacta
      121 gagtgcggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006330
VERSION     KHUT01006330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           88 g           45 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcttga
       61 caagtcggat gtgaaatccc tcggctcaac caaggaagtg catccgaaac tgtcgagctt
      121 gagtaccaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006331
VERSION     KHUT01006331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatgat
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tggtcatctg
      121 gagtccggaa gaggttggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga ggcgaaggcg gccaactggt ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006332
VERSION     KHUT01006332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           84 g           51 t
ORIGIN      
        1 tacccgcagc tcaagtggtg gtcattatta ttgggcctaa agcgtccgta gctggtttgt
       61 taaatgcctg ggtaaatcga tgcgctcaac gcatcgaatt ccgggtagac tggcaaactc
      121 gagaccggga gaggtgtagg ctactcgagg ggtaggggta aaatcctgta atccttcggg
      181 gaggaccagt ggcgaaggcg ctacactaga acggctctga cagtgaggga cgaagccctg
      241 gggcgcaaac cgg
//
LOCUS       KHUT01006333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006333
VERSION     KHUT01006333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           75 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggacttg
       61 taagtcagtg gtgaaatcct acagcttaac tgtagaactg ccattgatac tgctagtctt
      121 gagtacagtt gatgtggatg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat cgcgaaggca gttcacaaaa ctggtactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006334             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006334
VERSION     KHUT01006334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           81 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatga ttgggcgtaa agcatctgta ggtggtttct
       61 aaagtcaact gttaaatccc agagctcaac tttggccaag cagttgagta cttagagact
      121 tgagtacggt aggggtagag ggaattccta gtttagcggt gaaatgcata gagattagga
      181 agaacaccag tggcgaaagc gctctactgg gccgtgactg acactgagag atgaaagtta
      241 ggggagcgaa aagg
//
LOCUS       KHUT01006335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006335
VERSION     KHUT01006335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           88 g           54 t
ORIGIN      
        1 tacggagggt gccagcgttg tccggattca ttgggtttaa agggtgcgca ggcggaagac
       61 taagtcgggg gtgaaagacg gttgctcaac aatcgcagtg ccttcgatac tggttttctt
      121 gggtagagat gaggtgggcg gaattcaagg tgtagcggtg aaatgcatag ataccttgaa
      181 gaactccgat tgcgaaggca gcccgctggg ctcttaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006336
VERSION     KHUT01006336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           90 g           52 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggttgga
       61 caagtcagct gttaaagagt ggtgcttaac accataaagg cggttgaaac tgtttaacta
      121 gagtttggta ggggttgcgg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg cgcgactgga cctgaactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01006337             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006337
VERSION     KHUT01006337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttctt
       61 taagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tggagagctt
      121 tgagtgtggg agaggcaggc ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacacctg tgatgtagat ggcctgctgg accacaactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006338
VERSION     KHUT01006338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           86 g           51 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtttcg
       61 aaagtcagat gtgaaagccc tcggcttaac caaggaagtg catttgaaac ttcgagactt
      121 gaatattgga gagggtcgca gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcgacctggc caaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006339
VERSION     KHUT01006339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           86 g           56 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggcgggcttt
       61 taagtcgggt gtgaaatccc atggctcaac catggaattg cattcgaaac tggaagtctt
      121 gagtgtggga gagattggta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccggt ggcgaaggcg gccaattggc ccaacactga cgctgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006340             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006340
VERSION     KHUT01006340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgtta cccggattca ctgggcgtaa agcgcgtgta ggcggctggc
       61 caagcgtggt gtgaaatctc cgggctcaac ccggaaactg cactgcgaac tggcctggct
      121 ggagtgcagg agagggatgc ggaattcacg gtgtagcggt gaaatgcgta gatatcgtga
      181 ggaacaccag tggcgaaggc ggcatcctgg cctgtaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01006341             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006341
VERSION     KHUT01006341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           92 g           53 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggattta ctgggcgtaa agggtgtgta ggcggtcgtg
       61 caagtctggt gtgaaatctt caccactcaa cgggaagagg tcactggaaa ctgcatgact
      121 tgagtttggt agaggctagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacacctg tggcgaaggc ggctagctgg gccaatactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006342
VERSION     KHUT01006342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttaact gtgaaagccc tgggctcaac ctgggaatgc cagttaatac tgttgggcta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006343             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006343
VERSION     KHUT01006343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagcttgta ggcggttcgg
       61 caagtcgggt gtgaaaactc caggctcaac ctggagacgc cacccgatac tgctgtgact
      121 cgagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagaa gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006344
VERSION     KHUT01006344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtctgct gtgaaaactt ggggctcaac tccaagcctg cagtggatac gggctagcta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctgga ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006345
VERSION     KHUT01006345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           54 c          104 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgca ggcggccgag
       61 cacgtccgtt gtgaaatcgc ctggctcaac tgggcggggt cagcggatac ggcttggctg
      121 gagcaagcta gggggcaatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctgga gctttgctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01006346             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006346
VERSION     KHUT01006346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctctt
       61 taagtctgtc ggtcaaatgc cggggctcaa ccccgtgctc gccggagata ctggagagct
      121 ggagacatgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcatggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006347
VERSION     KHUT01006347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           95 g           45 t
ORIGIN      
        1 tacgagtggt gcgagcgtta atcggaatta ctgggcttaa agggtgcgca ggcgggtccg
       61 caagcacttc gtgaaatccc ccggctcaac cggggaactg cggggtggac tgcgggtctt
      121 gagagaggta ggggccgacg gaacgatcgg tggagcggtg aaatgcgtag atatcgatcg
      181 gaacgccaat ggtgaagaca gtcggctggg ccttttctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006348
VERSION     KHUT01006348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 gacggaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 taagttgggt gtgaaatccc tcggcttaac cgaggaattg cgcccaaaac tactgtgctt
      121 gagaaagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006349
VERSION     KHUT01006349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           88 g           48 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggcttaac cgtggaaccg cttggtatac tggcgatctt
      121 gagccaccta ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgcctat ggtgaaaaca gctcgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006350
VERSION     KHUT01006350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggttgcc
       61 taagtcagat gtgaaatccc ggggctcaac ctcggaactg catttgatac tgggtaactt
      121 gagtactgga ggggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006351
VERSION     KHUT01006351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c           94 g           55 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ttgggcgtaa agcgcacgca agcggtcttt
       61 caagtctgac gtgaaatctc ttggcttaac tgggagcggt cgttggaaac tgagaggctt
      121 gaggcgatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaatacctgt ggcgaaagcg gcaccctggc gtcgttctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006352
VERSION     KHUT01006352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          101 g           40 t
ORIGIN      
        1 gacagagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatcgg
       61 caagtccgga gtgaaatccc gccgctcaac ggcggggctg ctttggaaac tgcaggtcta
      121 gggagcggta gaggtcggta gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaatacccgt ggcgaaggcg gccgactggg ccgtctccga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006353
VERSION     KHUT01006353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcctgg
       61 taagtccgtg gtgaaagcct cttgctcaac aagagaactg ccatggatac tgccgggctt
      121 gagtccagac gaggttggcg gaatagaggc tgtagcggtg aaatgcatag atagcctcta
      181 gaaccccgat tgcgtaggca gctgactagg ctggtactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006354
VERSION     KHUT01006354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctgg
       61 taagtctggg gtgaaagccc gttgctcaac aacggaactg ccctggaaac tgccagactt
      121 gagtacagac gagggtggcg gaatggatgc tgtagcggtg aaatgcatag atagcatcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006355
VERSION     KHUT01006355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctcg
       61 tttgtcaggg gtgaaagcct tgggcttaac ccaagaagtg cccctgaaac ggcgagactc
      121 gagttctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagatactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006356
VERSION     KHUT01006356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggccgct
       61 caagtcaggt gtgaaagccc cgggctcaac ctgggaactg cacttgatac tgagtggcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006357
VERSION     KHUT01006357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          106 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcgttg
       61 caagtcggcc gtgaaaacgc caggctcaac ctggcgaggt cgggcgagac tgcaaggcta
      121 gaggtaggca gagggcggtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctggg cctatcctga cgctgaggtg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01006358             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006358
VERSION     KHUT01006358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           37 c           81 g           58 t
ORIGIN      
        1 gacataggtg gcaaatgtta tccggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttactg gtgggaaatc ggggcccaac cccgtggaag ttggtaatac tattagacta
      121 gaggatcgga gaggttaacg gaattctatg tgtagcggta aaatgcgtaa atatatagag
      181 gaacaccaaa aggcgaaggc agttaactat tcgattcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01006359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006359
VERSION     KHUT01006359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttttg
       61 taagtcagct gtgaaatccc cgggctcaac ctgggaatgg cagatgagac tgcaaggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006360
VERSION     KHUT01006360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          109 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtggct
       61 gatgtgccgt gtgacaggcg ggggctcaac ccccgttggg catggtagac agggtcactg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01006361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006361
VERSION     KHUT01006361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           84 g           58 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggttaat
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaagtg cactcaaaac taattagctt
      121 gagagtggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccgga ggcgaaggcg gccttctgga ccatttctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006362
VERSION     KHUT01006362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           97 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtccggg gtgaaagcct ccagcttaac tggagaagtg ccttggatac tggcgagctc
      121 gagcgaggta ggggcgggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acccgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006363
VERSION     KHUT01006363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacgtt
       61 taagtcgggg gtgaaatcct gaggctcaac ctcagaattg ccttcgatac tgggcgtctc
      121 gagaccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006364
VERSION     KHUT01006364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggtctat
       61 taagtcaagc gtgaaagccc tgggctcaac ccgggaattg cgtttgatac tggtaggctt
      121 gagattcaga gaggtgagtg gaattcctag tgtagcagtg aaatgcgtag atattaggag
      181 gaacatcagt ggcgaaggcg gctcactggc tgaacactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006365
VERSION     KHUT01006365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggggtga
       61 caagtggtgt atgaaatatc ccagcttaac tgggaagggt tatgccagac tgtcattcta
      121 gagtgcgaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006366
VERSION     KHUT01006366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           76 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtgggatga
       61 taagtcagag gtgaaagcta gcagcttaac tgttaaattg cctttgatac tgttattctt
      121 gagtatagtt ggcgttggcg gaatgtgaca tgtagcggtg aaatgcgtag atatgtcaca
      181 gaacaccgat cgcgaaggca gctaacgaaa ctatcactga cactgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006367
VERSION     KHUT01006367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggtggtcact
       61 caagtgtcgt gtgaaagcgg cccgcttaac gggtcgaggc catggcagac tgtgtgactg
      121 gaggtcgtga gaggtccgtg gaattgccgg tgtagtggtg acatgcgtag atatcggcag
      181 gaacacccaa ggggaagcca gcggactggc acgatgctga cactgaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006368
VERSION     KHUT01006368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           86 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggagat
       61 taagtcaggg gtgaaagacg gttgctcaac aatcgcagtg cccttgatac tggttttctt
      121 gggtggggtc gaggtagatg gaattcaagg tgtagcggtg aaatgcatag ataccttgaa
      181 gaactccgat tgcgaaggca gtctactagg ccttaaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006369
VERSION     KHUT01006369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           62 c          109 g           33 t
ORIGIN      
        1 tacggagggc gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccgcg
       61 aaagtcgctg gtgaaagccc ggggctcaac cccgggtcgg ccagtgatac tgcgcggctg
      121 gagcactgta ggggcgggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006370
VERSION     KHUT01006370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c          100 g           39 t
ORIGIN      
        1 gacggagggc gcgaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggatcgg
       61 taagtcgggg gtgaaagccc ggggctcaac tccgggactg cctctgaaac tgccggtcta
      121 gagtgcagga gaggaaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006371
VERSION     KHUT01006371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           47 c           98 g           54 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agagctcgta ggcggctact
       61 cgagtcgggt gtgaaatccc atggcttaac tgtgggtctg cattcgatac tgggtggctt
      121 gagtctggta ggggagtctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006372
VERSION     KHUT01006372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           98 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatcct ccggcttaac cggagaagtg cttttgatac tggcgagctt
      121 gagggaggta ggggcaggcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006373
VERSION     KHUT01006373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           94 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaattg cattcgatac tgtcaggctc
      121 gagtctagga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gcttcctggc ctaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006374
VERSION     KHUT01006374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           84 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggggcga
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagcattg ccattgatac tgtcgtcctt
      121 gagtacgctt gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccaat tgcgaaggca gctcacgaag gcgaaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01006375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006375
VERSION     KHUT01006375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacagaggtg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccctg
       61 taagtcaacg gtgaaatccc tcggctcaac cgaggacgtg cctttgatac tgcagggctc
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006376
VERSION     KHUT01006376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggcaggta ggtggtcatg
       61 taagtccggt gtgaaatccc agggctcaac cctggaactg cattggaaac tccatgactt
      121 gagtaccgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cggttactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01006377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006377
VERSION     KHUT01006377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           99 g           43 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggatgg
       61 caagtcggtc gtgaaatcct tcggcccaac cgaagaacgt cgatcgatac tgctgttcta
      121 gagggttgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caactcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006378
VERSION     KHUT01006378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtcctt
       61 taagttagat gtgaaagccc aaggctcaac cttggaattg catttaatac tgaagggctt
      121 gagttttgga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccaat ggcgtaggca gctatctggc caaaaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006379
VERSION     KHUT01006379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           47 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcgact gtgaaaactc ggggcttaac cctgggcctg cagtcgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006380
VERSION     KHUT01006380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgagta ggcgggtttg
       61 caagtctgcg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tgtgaatctc
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaatca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006381
VERSION     KHUT01006381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtgagg
       61 caagtctgat gtgaaagctt cgagcttaac tcgaaaacgg cattggaaac tgcctcgctt
      121 gaggcttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag agatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006382
VERSION     KHUT01006382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           37 c           80 g           59 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtaatg
       61 taagtctgat attaaagacc aaagcttaac ttcgggactg tattggaaac tgcattacta
      121 gaggacaata gaggaagttg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg aacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006383
VERSION     KHUT01006383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           95 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggtcggc
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgagac tggcggactt
      121 gagtgcagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctgttactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006384
VERSION     KHUT01006384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgta ggcggtttag
       61 taagtcgggt gtgaaatcct cgggcttaac ccgagaactg cgcccgaaac tgcttcactg
      121 gagggacgga gaggtgcata gaattcccgg tgtaggggtg aaatccatag agatcgggag
      181 gaataccaat ggcgaaggca gtgcactgga cgtcacctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006385
VERSION     KHUT01006385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggaatca ttgggcttaa agcgtgcgta ggcggacatg
       61 caggcattgt gtgaaatccc tcggctcaac cggggaactg cacagtgaac cgcatgtctt
      121 gaggtacaca gaggtggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggccactggg tgtattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006386
VERSION     KHUT01006386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           94 g           48 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctcg
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg cctcggaaac ggcgagactg
      121 gagtcttgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caagtactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006387
VERSION     KHUT01006387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggccggg
       61 gaagtctggt gtgaaatctc tgagcttaac tcagaaatgg cactggaaac tctctggctt
      121 gaggatcgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgagccctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006388
VERSION     KHUT01006388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggttttc
       61 taagtctgat gtgaaatccc agggcttaac cctggaactg cattggatac tggataacta
      121 gagggcgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccatctggc tcgcacctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006389
VERSION     KHUT01006389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctgcc
       61 caagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgagac tgggcggctt
      121 gagtttcgga gaggtgggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcccactgga cgaacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006390
VERSION     KHUT01006390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggattta ctgggcgtaa agcgtacgca gacggccacg
       61 caggtcgggt gtgaaagccc cgggctcaac ccgggaattg cattcgaaac catgtggctc
      121 gagtgtggga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gctacctggt ccacaactga cgttcaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006391
VERSION     KHUT01006391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           46 c           76 g           56 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggactaa
       61 taagtcagtg gtgaaagccg gtagcttaac tatcgaactg ccattgaaac tgctagtctc
      121 gagtacggtt gaggtggctg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaggca agtcactaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006392
VERSION     KHUT01006392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatca ttgggcgtaa agagcacgta ggcgggtttg
       61 taagtcgatt gtgaaatccc ggggcttaac ctcggaattg cagtcgaaac tgcagatctt
      121 gaatgcttga gagggtggga gaattcctgg tggagaagtg aaattcgtag agatcaggag
      181 gaatacctga ggcgaaggcg cctgcctggc aagacattga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006393
VERSION     KHUT01006393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggcgcg
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tatgcgtctt
      121 gaattttgtt gaggttggcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccgat tgcgaaggca gctaactaag caaacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006394
VERSION     KHUT01006394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          104 g           41 t
ORIGIN      
        1 tacggggggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtttga
       61 taaggctgtg gtgaaagccc ggggctcaac cccgggtctg ccatggcgac tgtcagactg
      121 gagggctcga gaggcagacg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaagaccggt ggcgaaggcg gtctgctggc gagtacctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006395
VERSION     KHUT01006395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           85 g           46 t
ORIGIN      
        1 gacgaaccgc acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac taccgtgctt
      121 gagggagata gaggtgagtg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaagcg actcactgga tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006396
VERSION     KHUT01006396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           81 g           65 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggagcgta ggcgggttgt
       61 taagtcagtt gtgaaagttt gcggctcaac cgtaaaattg cagttgatac tggcatcctt
      121 gagtacagta gaggtaggcg gaattcgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gcctgctgga ctgtaactga cgctgatgct cgaaagtgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01006397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006397
VERSION     KHUT01006397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           95 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacga
       61 caagtcggaa gtgaaagccc ggggctcaac cccggaacgt ctttcgaaac tgtcgagcta
      121 gagtgcagaa gaggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006398
VERSION     KHUT01006398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           75 g           61 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ctgggtttaa agggtgcgta ggtggacctg
       61 taagtcagtg gtgaaagccc gaagctcaac ttcggaactg ccattgatac tgcttgtctt
      121 gagtatagtt gaggtaggcg gaatatgtca tgtagtggtg aaatacttag atatgacata
      181 gaacaccgat tgcgaaggca gcttgctaag ctataactga cactgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006399
VERSION     KHUT01006399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgatc
       61 taagtcgggt gtgaaatctc cgggcttaac ccggagggtg cgcccgaaac tggactgctc
      121 gagggtggga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga ccactcctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006400
VERSION     KHUT01006400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtcttg
       61 tttgtctgtg gtgaaatcct tgggcttaac ccaagaagtg ccatagaaac ggcaagactc
      121 gagttctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagatactga cactcaacta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006401
VERSION     KHUT01006401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           44 c           96 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggtttgg
       61 tttgttaggt gtgaaattcc tgggcttaac ctgggggctg cacttaatac ggccaggctt
      121 gagtgtgaaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006402
VERSION     KHUT01006402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          104 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtccct
       61 taagcgtgtg gtgaaagctc ggggctcaac ctcgagtcgg ccatgcgaac tgggggactg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006403
VERSION     KHUT01006403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           75 g           60 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaagccc caggcccaac ctgggaattg ccattgatac tgatgatctt
      121 gagtacattt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgtaggca gcttactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006404
VERSION     KHUT01006404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           82 g           51 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtttttggc
       61 cacgtctggc gtgaaatttc ggggctcaac cccgttccca cgctggaaac gagctagata
      121 gagtcattca ggggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agtgaagaca ggatgctagg aatgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01006405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006405
VERSION     KHUT01006405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggccaat
       61 caagtcaggg gtgaaatgcc ggagcttaac ttcggaactg cctttgaaac tgattggctg
      121 gaagatgaga ggggatagcg gaatttctgg tgtagaggtg aaattcgtag ataccagaag
      181 gaacaccggt ggcgaaggcg gctatctggc tcattcttga cgctctggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006406
VERSION     KHUT01006406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggctgt
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tatcagtctt
      121 gaatatcgtt gaggtgggca gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaataccgat tgcgaaggca gctcactaag cgtgtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006407
VERSION     KHUT01006407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           86 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtcggat gtgaaatctc taggctcaac ctagaaatgg catcggaaac taccctgcta
      121 gaggtttgga gggggggctg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006408
VERSION     KHUT01006408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           54 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggcgatt
       61 caagtcgggg gtgaaatccc agagctcaac tctggaactg ccttcgatac tgtcttgcta
      121 gagtgtggaa gaggttggtg gaactgcaag tgtagaggtg aaattcgtag atatttgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccattactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006409
VERSION     KHUT01006409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttagt
       61 taagtcgggt gtgaaatccc tcagctcaac tgaggaagtg catccgaaac tggctagcta
      121 gagaatggta gagggaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gcctcctgga ccatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006410
VERSION     KHUT01006410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 tgtgtcggat gtgaaatccc tcggcttaac caaggaagtg cattcgaaac tgcgaagctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006411
VERSION     KHUT01006411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgtt
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catctcatac tgggcagcta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006412
VERSION     KHUT01006412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 tacggggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcagcg
       61 taagtcaggt gtgaaagccc tgggcttaac ccaggaagtg catctgatac tgcgtagctc
      121 gagtatggga gagggtagag gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctacctgga ccaatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006413             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006413
VERSION     KHUT01006413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           49 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcggat gtgaaacctc caggcttaac ctggagacgc cattcgatac tgctatggct
      121 tgagtccggt aggggcgcat ggaattcccg gtgtagcggt gaaatgctta gatatcggga
      181 ggaacaccag tggcgaaggc ggtgcgctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006414
VERSION     KHUT01006414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggctagt
       61 caagttcgac gtgaaagctc ccggcttaac tgggagaggt cgttgaaaac tggttagctt
      121 gaggcaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctagc gttgttctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006415
VERSION     KHUT01006415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttagg
       61 taagtgggat gtgaaagccc ccggctcacc cggggatctg cattccatac tgcccgactt
      121 gagtatggga gaggataggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cctatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006416
VERSION     KHUT01006416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           76 g           65 t
ORIGIN      
        1 tacggaggat cctagcgttg tccggattta ctgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaagttt gcagcttaac tgtaaaattg ccattgaaac tgataatctt
      121 gagtacggtt gaggtggatg gaatgtgtca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gttcactaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006417
VERSION     KHUT01006417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggcttgt
       61 cacgtcgggt gtgaaatccc agggcttaac cctgggtctg cattcgatac gggctggcta
      121 gggtccttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aaggtaccga cgctgaggag cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006418
VERSION     KHUT01006418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           90 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatca ttgggcgtaa agcgtccgta ggtggttcat
       61 caagtctgct gtcaaagcac aaggcttaac cttgtaaagg cggtggaaac tgatgggcta
      121 gagtgaggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaagcg ctctactgga cctcaactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01006419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006419
VERSION     KHUT01006419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           60 c           97 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcgga
       61 caagtcgggg gtgaaatcgt tcggcccaac cggacaaggg cccccgatac tggccgcctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006420
VERSION     KHUT01006420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcgggtgtg
       61 taagtaggga gtgaaaggtt ggggctcaac cctaacacgg ctccctatac tgcacgtctc
      121 gaggatcgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cgatgcctga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01006421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006421
VERSION     KHUT01006421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agagctcgta ggcggccgtc
       61 tgcgtcggat gtgaaagctc agggctcaac tctgagatgc cattcgatac ggggcggcta
      121 gaggtaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01006422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006422
VERSION     KHUT01006422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          101 g           47 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggactta ctgggcgtaa agcgtgggta ggtggtcggt
       61 gatgtgccgt gtgaaagccc cgcgttcatc ggggggaggt cgcggtagac acattgactt
      121 gaggcataca gagggccgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gcggcctggg tatgacctga cactgagcca cgacggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006423
VERSION     KHUT01006423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccgc
       61 caagtccggg gtgaaatcct cccgcccaac gggagaagtg ccttggatac tggcgggctc
      121 gagcggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccccccctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006424             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006424
VERSION     KHUT01006424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           46 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 taagttgggt gtgaaacctc caggcttaac ctggagacgc cactcaatac tgccatggct
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006425
VERSION     KHUT01006425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           83 g           58 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggttagg
       61 taagttagat gtgaaatccc cgggctcaac ctgggaattg cgtttaaaac tgtttgacta
      121 gagtattgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctggg caaatactga cactgagaca cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006426
VERSION     KHUT01006426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg atcggaatta ctgggcgtaa agggcgcgta ggcggtaaag
       61 tatgtgtggt gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tacttaacta
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006427
VERSION     KHUT01006427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtgggtgaa
       61 aaagttggat gtaaaatccc cgggcttaac ctgggaaggt catccaagac tgttcgtcta
      121 gagtacagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006428
VERSION     KHUT01006428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           93 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 taagtccgcg gtgaaatcct ccagctcaac tggagaactg ccgcggatac tggtcgtctc
      121 gaggaacgta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga cgttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006429
VERSION     KHUT01006429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           55 c          108 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggttcgt
       61 tgcgtcgtgc gtgaaggcct tcggctcaac cggaggtggt cgtgcgatac gggcgaactg
      121 gaggtaggta gggggacgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggc ggcgaaggcg gcgtcctgga cctggcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01006430             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006430
VERSION     KHUT01006430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           87 g           52 t
ORIGIN      
        1 gacagaggtg gctagcgttg ttcggattca ttgggtgtaa agggtgcgta ggcggtttag
       61 aaagttaaat gtgaaagccc acagctcaac tgtggaaatg catttaaaac tcctagacta
      121 gagtgcgaga gaggtgaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt agcgaaggcg cttcactggc tcgtaactga cgctgagtgc acgaaggtat
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006431             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006431
VERSION     KHUT01006431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgctttaa ggcggttcgc
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cactcgatac tggcgtgact
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtggtctgg gccggaactg acgctgtaga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006432
VERSION     KHUT01006432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           81 g           54 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggcggtg
       61 ttagtctttt gtcaaagctc cgagctcaac ttggaaaagg cagaagaaac ggcactgcta
      121 gagaatgtga gaggtttgcg gaactaatgg tgtaggggtg aaatccgttg atatcattgg
      181 gaacaccaaa tgcgaaggca gcaaactggc acatttctga cgctcaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01006433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006433
VERSION     KHUT01006433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          103 g           35 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggccagg
       61 tcagtcgggc gtgaaagccc ggggctcaac cccggaacgg cgcccgagac ggcccggctc
      121 gagaccggga gaggagggtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006434
VERSION     KHUT01006434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           83 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggaccat
       61 taagtagaaa gtgaaaggtt ggagctcaac tccgacactg cttcctatac tggtggtctt
      121 gagtttcgga gggggaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca gcttcctgga cgaatactga cgctcaggca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01006435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006435
VERSION     KHUT01006435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agcgcttgta ggtggccggc
       61 taagttggac gtgaaaaccc cgggctcaac tcggggcggc cgttcaatac tggcgggctg
      121 gagggcgtca gagggcagtg gaatccctgg tgtagcagtg aaatgcgtag atatcagggg
      181 gaacacccgt ggcgaaggcg gctgcctggg acgcgcctga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006436
VERSION     KHUT01006436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttcgt
       61 taagattgtt gtgaaatccc tgggcttaac ctaggaactg caacgaagac tggcgagctg
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006437
VERSION     KHUT01006437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           89 g           46 t
ORIGIN      
        1 aacgtagggt gcaagcgttg tccggaatta ctgggtgtaa agggagcgca ggcggaccgg
       61 caagttggaa gtgaaaacca tgggctcaac ccatgaattg ctttcaaaac tgctggcctt
      121 gagtagtgca gaggtaggcg gaattcccgg tgtagcggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcctactggg caccaactga cgctgaggct cgaaagtatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006438
VERSION     KHUT01006438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacgggggat gcgagcgtta tccggattca ttgggtttaa agggtgcgta ggcggctttg
       61 caagtcagcg gtgaaaggcc cgggctcaac ccggaaagtg ccgttgatac tgtgaggctg
      121 gaatgcggat gctgtgggag gaatgagtgg tgtagcggtg aaatgcatag atatcactca
      181 gaacaccgat tgcgaaggca tctcacaaat ccgcaattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01006439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006439
VERSION     KHUT01006439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttcg
       61 taagtcgtgt gtgaaatccc ccggctcaac tggggaactg cacacgaaac tgcgaggctt
      121 gaattcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006440
VERSION     KHUT01006440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           99 g           37 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggcgcgg
       61 cgcgtggggt gtgaaagctg gcggcttaac tgccagatgc catcccatac ggccgggctc
      121 gagccctgca gaggtccatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg caggagctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006441
VERSION     KHUT01006441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           94 g           50 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggccat
       61 taagtgagag gtgaaattcc agggctcaac cccggaactg cctctcatac tgttggtctt
      121 gagtgaggta gagagaagtg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg cctttactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006442
VERSION     KHUT01006442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtttct
       61 taagttcgtc gtgaaatcgc ccggcttaac cgggcgcggt cgatggatac tgggagactt
      121 gagggcggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cgctaagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006443
VERSION     KHUT01006443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           80 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtcgg
       61 taagtccgtg gtgaaatccc cgagcttaac tcgggaactg ccgtggatac tatcgacctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttactaca cagtcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006444
VERSION     KHUT01006444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           84 g           54 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatga ctgggcgtaa agggtgcgta gacggtatat
       61 taagtctgtt gttaaattct tcggcctaac cgaagtcaag cgatggaaac tggtattctg
      121 gagggtgaaa gagaggagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaagca gctccttggt tcacacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006445
VERSION     KHUT01006445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggtggcctgt
       61 taagtggtgt atgaaatcgc ccggctcaac cgggcggggt tatgccagac tggcaggcta
      121 gagggtggga gagggatgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcatcctggc ccacgactga cactcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006446
VERSION     KHUT01006446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          105 g           35 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtga ctgggcgtaa agggcgtgca ggcggcgctg
       61 caagtggctc gtgaaagacc ccggctcaac cggggggaga cgggtcagac tgcagggctg
      121 gaggatgtca gagggacacg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaggcg gtgtcctggg gcgtacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006447
VERSION     KHUT01006447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          102 g           39 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtttgg
       61 taagtggggt gtgaaagcgg gcggcttaac cgcccgaggc catctcagac tgcccgactc
      121 gagccgttca gaggtccctc gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaagacccac ggggaagcca ggggactggg gacgtgctga cgctcaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006448
VERSION     KHUT01006448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctgat gtgaaatctc caagcttaac ttggaacccg catcagggac tgccgtgctt
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccactactga cgctgaggcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006449
VERSION     KHUT01006449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggttact
       61 taagtcggat gtgaaagccc ttggctcaac tgaggaagtg catccgaaac tgagtagctt
      121 gagtatgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcaaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006450
VERSION     KHUT01006450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgttg ctcggaatta ctgggcgtaa agggtccgta ggcggcttca
       61 taagttagat gtgaaagctc agggctcaac cctgaaattg catctaaaac tgtggagcta
      121 gagtccagaa gaggtcggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactagt ctggcactga cgctgaggga cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006451
VERSION     KHUT01006451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggactgt
       61 taagtacctt gtgaaatccc ccggctcaac cggggaactg ctcggtatac tggcggtctt
      121 gagccactta ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggtgaaaaca gacgactggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006452
VERSION     KHUT01006452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           90 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgca ggcgggacag
       61 ttagtcagag gtgaaatcct gcggcttaac cgtagaattg cctttgatac tgctgttctt
      121 gagtcctgga gaggtcattg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga ggtgtagacg gatgactgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006453
VERSION     KHUT01006453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          102 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgta ggcgggggag
       61 caggtcggat gtgacagcgc gcggcttaac tgcgcgtggc cgttcgaaac cgcttttctt
      121 gaatgggggc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa cgcgaaggca gccggctagc cccagattga cgctgaggga cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006454
VERSION     KHUT01006454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctggt gtgaaaaccc gaggctcaac ctcgggctgg catcgggtac gggcaggcta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01006455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006455
VERSION     KHUT01006455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           80 g           59 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggccat
       61 taagtcaggg gtgaaagtct gcggctcaac cgtagaattg cccttgatac tggtggcctt
      121 gagttatggt gaagtaacta gaatatgtgg tgtagcggtg aaatgcatag atatcacata
      181 gaataccgat tgcgaaggca ggttactaac catatactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006456
VERSION     KHUT01006456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcggat gtgaaatccc ggggctcaac cccggaagtg cattcgaaac tgcctagcta
      121 gagttctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg accttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006457
VERSION     KHUT01006457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c          100 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcttgt
       61 atagtcggat gtgaaattcc tgggcttaac ctgggggctg catacgagac gtatgggctt
      121 gagtgcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006458
VERSION     KHUT01006458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcga
       61 caagtccgtg gtgaaagttc gacgctcaac gtcgaatcgg ccatggatac tgttgggctt
      121 gagcactgta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006459
VERSION     KHUT01006459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgac
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaatag cctctgatac tggttgtctt
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006460
VERSION     KHUT01006460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagag gtgaaagacg gttgctcaac aatcgcagtg cctttgatac tgaagagctg
      121 gagtgcagct gaggtaggcg gaatacaggg tgtagcggtg aaatgcatag ataccctgta
      181 gaacaccgat tgcgaaggca gcttactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006461
VERSION     KHUT01006461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           44 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtga ctgggcgtaa agggtccgta ggcgggaagg
       61 tcagccggtc gtgaaatctc ctggctcaac taggaggagt cggtcggaac tgccattctg
      121 gaatccgtgc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacgccaaa ggcgcaggca actggctagc acgagattga cgctgaggga cgaaggcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006462
VERSION     KHUT01006462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtttga
       61 aaagtcaggt gtgaaagccc cgggctcaac tcgggaagtg cacttgaaac tctcaagcta
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaacactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006463
VERSION     KHUT01006463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           53 c           81 g           44 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccgaaatga ttgggcgtaa agcgtatgta tgtggctttt
       61 taagtccgcc gtcaaatccc agggctcaac cctggacaga cggtggaaac taccaagcta
      121 gagtacgata ggggcagagg gaatttccgg tggagcggtg aaatgcattg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggcgcaaat ggg
//
LOCUS       KHUT01006464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006464
VERSION     KHUT01006464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           93 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcgggatgt
       61 taagtctgac tttaaagacc atggctcaac catgggagtg ggctggatac tggcattcta
      121 gacaactgga gaggcgattg gaattactgg tgtagcggtg gaatgcgtag ataccagtag
      181 gaacgtcaat ggcgaaggca ggtcgctgga cagtttgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006465
VERSION     KHUT01006465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaaggg ccttggaaac tgctggtctt
      121 gagggaggta ggggtccgtg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaacgccggt ggcgaaagcg acggactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006466
VERSION     KHUT01006466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 gaagtcagtg gtgaaatctg tgggcttaac ccacaaactg ccgttgatac tctctgtctt
      121 gaatattgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgctaca catatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006467
VERSION     KHUT01006467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtgca
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatgcgtctt
      121 gaatcttgtt gaggagggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctctctaag caagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006468
VERSION     KHUT01006468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          105 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctttg
       61 caaggctgcg gtgaaagcct ggggctcaac cccaggtctg ccgtggcgac tgtgaggctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006469
VERSION     KHUT01006469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcgt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg ccttcgatac tggcgatctt
      121 gagttcggga gaggttggtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gccaactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006470
VERSION     KHUT01006470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           90 g           52 t
ORIGIN      
        1 tacagaggtc tcgagcgttg atcggaatta ttgggcttaa agggtgcgta ggcggtcctg
       61 tcagtgcctt gtgaaatccc atggctcaac catggaattg ctcggtagac cacaggactt
      121 gaggtcggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006471
VERSION     KHUT01006471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           89 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcagtg gtgaaaccct tgggcttaac ccaaggagtg ccattgatac tgcaaggctt
      121 gggtcatctt tgggcgggcg gaatggatgg tgtagcggtg aaatgcatag atatcaacca
      181 gaacgccgat agcgaaggca gctcgctgag gatgtaccga cgctgaggca cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006472
VERSION     KHUT01006472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgcgagg
       61 agggtggggt gtgaaatccc tcggcttaac cgaggaattg cgctccaaac cgccttgctg
      121 gagtgagaca gaggtgagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaacaccaga ggcgaaggcg gctcactggg tctcaactga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006473
VERSION     KHUT01006473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtggctttg
       61 taagtcagtg gtgaaatatt gcggctcaac cgcaaaactg ccattgatac tgcttagctt
      121 gaatcacgtc gaggtggatg gaataataca tgtagcggtg aaatgcttag atatgtatta
      181 gaacaccgat tgcgaaggca gttcactagg cgtgtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006474             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006474
VERSION     KHUT01006474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           83 g           53 t
ORIGIN      
        1 tacgagaacc tcaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtcttgt gtgaaagcct gtcgctcaac gacagatccg catgggaaac ggcacaactg
      121 agagggtgca agaggtgaat ggaactcatg gtgtaggggt gaaatccgtt gatatcatgg
      181 ggaacgccaa aagcgaaggc aattcactgg tgcatacctg acgctgaaac acgaaagcca
      241 gggtagcgaa tggg
//
LOCUS       KHUT01006475             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006475
VERSION     KHUT01006475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           53 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggcctgt
       61 taagtcggat gtgaaagccc tcagctcaac tgaggaattg cattcgatac tggcaggctt
      121 gagtgcgata gaggaaagcg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacctgt ggcgaaggcg gctttctggg tcgtaactga cgctgaggca cgaaagcgtt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01006476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006476
VERSION     KHUT01006476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggcttgg
       61 taagttgaat gtgaaatccc cgggctcaac ctgggaactg catccaaaac tgcctggcta
      121 gagtacggta gagggtggtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacaccagt ggcgaaggcg accacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006477
VERSION     KHUT01006477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgggtgta ggcggatctc
       61 taagtcgggg gtgaaagccc agggctcaac cctggaattg ccttcgatac tggagatctc
      121 gagtctagga gaggtgagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga ggcgaaggcg gctcactgga ctagtactga cgctgagacc cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006478
VERSION     KHUT01006478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggttttc
       61 tacgttcatg gtgacagtct ccggcttaac tggggaagcg ttttgaaaac gggaaaactt
      121 gagaacgaga gagggacacg gaactccgag tggagcagtg aaatgcgtag agattcggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtttctga cgctcaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006479
VERSION     KHUT01006479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          100 g           46 t
ORIGIN      
        1 tacgtaggag actagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggcatac
       61 caagtctgtt gtgaaatcgc ccggctcaac tgggcggggt caatggaaac tggtaagctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gctcgctggg ccgatcctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006480
VERSION     KHUT01006480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           47 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ttgggcgtaa agcgctcgca ggcggctttt
       61 caagtctgcc gtgaaatcgc tcggcttaac cgggcggggt cggtggaaac tgaggggctt
      121 gaggcgcgga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacatcagt ggtgcaaacg gcaccctggc cgtagcctga cgctcaggag cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006481
VERSION     KHUT01006481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           90 g           45 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggcctac
       61 taagttggat gtgaaatctc ctggctcaac tgggagagga cgttcaaaac tggcgggctc
      121 gaggacggca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gccttctggg ccgttcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006482
VERSION     KHUT01006482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaatgg catttgaaac tggcaggctg
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006483
VERSION     KHUT01006483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtttgc
       61 taagttggat gtgaaaactc tgggcttaac tcagagcctg cattcaaaac tgacaagctc
      121 gagttctgga gggggtagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagaaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006484
VERSION     KHUT01006484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           82 g           55 t
ORIGIN      
        1 tacgagggcc ccgagcgtta tccggattta ttgggcgtaa agagtgcgca ggtggttttg
       61 cgcgtctgtt gttaaatgtc actgctcaac agtgtcgctg caatggatac ggcaaaacta
      121 gagcatgtta gaggttactg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca ggtaactgga acattgctga cactcaggca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01006485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006485
VERSION     KHUT01006485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           58 c          109 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gaagtcgggg gtgaaagtcc gaggctcaac ctcggatcgg ccttcgagac tttcgcgctg
      121 gagcaccgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cggtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006486
VERSION     KHUT01006486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           90 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtggac
       61 taagtcaggt gtgaaatctc cgggctcaac ccggagtctg cgcccgatac tggttcgcta
      121 gaggatggga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacccgt ggcgaaggcg agtctctgga ccattcctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006487
VERSION     KHUT01006487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           97 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggtgtaa agggcgcgta ggcggtgtga
       61 tgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg cattgcagac tatcgcactg
      121 gagtacatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006488
VERSION     KHUT01006488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggtcttc
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaattg ccattgaaac tgtgaggctt
      121 gaggacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgtttctga cgctcatgca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006489
VERSION     KHUT01006489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtctggg gtgaaatctt tcggctcaac cggaaaagtg cctgggatac tggcaagctc
      121 gagggaggta ggggcatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006490
VERSION     KHUT01006490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           76 g           61 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggaatta ttgggcgtaa agcgtctgca ggtggacttt
       61 taagtctttt gttaaagctc tgggcttaac ctagaatctg caaaagaaac tatttgtctt
      121 gagtatggta gaggtaaagg gaatttccag tggagcggtg aaatgcttag atattgggaa
      181 gaacaccaac ggcgaaggca ctttactggg ccagcactga cactgagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006491
VERSION     KHUT01006491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggtggcttgt
       61 taagtcggtt gtgaaatccc atggctcaac catggaagtg ctgccgaaac tggcgagcta
      121 gagtcccgac gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg agggtactga cactcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006492
VERSION     KHUT01006492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 caagtggtgt gtgaaatgcc ctggctcaac cagggaactg cgcaccaaac tgccgagctt
      121 gagggctgta gaggaagctg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgtt ggcgaaggcg agcttctggg cagtccctga cgctgaggcg cgaaagttgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006493
VERSION     KHUT01006493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           78 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttag
       61 taagtcagtg tttaaagtca atagctcaac tattggtatg gcattgatac tgctgaactt
      121 gagtaaagtt gaggtgggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gctcactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006494
VERSION     KHUT01006494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggatttg
       61 taagtcaggt gtgaaatctc ggggctcaac ctcgaaactg cgcctgaaac tacaagtcta
      121 gaatgtcgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggt ggcgaaggcg cctacctgga cgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006495
VERSION     KHUT01006495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           83 g           54 t
ORIGIN      
        1 tacggagggt ccaaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggctttg
       61 taagtgtacg attaaagacc gacgcctaac gtcgggagtg ttgtacatac tgcatagctt
      121 gagaatatca ggggcaagcg gaactttggg tgtaggggtg aaatccgtag atatccaaag
      181 gaacaccaat ggcgaaggca gcttgctagg gtatttctga cactgagatg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006496
VERSION     KHUT01006496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggattta ctgggcgtaa agcgcacgta ggcggatcag
       61 aaagttaggg gtgaaatccc ggggctcaac ctcggaactg cctctaaaac tcctggtctt
      121 gaggtcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006497
VERSION     KHUT01006497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           82 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggatgct
       61 taagtgtgat gttaaatcgt gtggctcaac catatcgatg catcgcatac tgggtatcta
      121 gaggacggca gaggcaagtg gaactcgtag tgtagcagtg aaatgcgtag atattacgaa
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006498
VERSION     KHUT01006498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 tcagtctgac gtgaaatctc cgggcttaac ccggaaatgg cgttggaaac taccttgctt
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006499
VERSION     KHUT01006499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggttaga
       61 taagtcgtgt gttaaagccc ccggctcaac tgggggaggt catgcgagac tatttgactt
      121 gagggcagca gaggagcatg gaactcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg gtgctctggg ctgcaactga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006500
VERSION     KHUT01006500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           55 c           74 g           48 t
ORIGIN      
        1 tacagagacc ccaagcatta tccggaatta ctgggcgtaa agcgcgagca gccggtcatg
       61 ttagtctgat gtaaaaccac agggcctaac cctgtgcctg cattggaaac ggcatgactt
      121 gagcgaataa gaggagggca gaacgtatgg tgtaggggtg aaatccactg atatcatacg
      181 gaataccaaa agcgaaggca gccctctagt gttctgctga cggtcaatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01006501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006501
VERSION     KHUT01006501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgat
       61 cgagtctggg gtgaaatcct ctcgcttaac gagagaactg ccccggatac tggtcatctc
      121 gaggaaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006502
VERSION     KHUT01006502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           97 g           45 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgct
       61 taagtcggat gtgaaaaccc gaggctcaac ctcgggcctg cattcgatac tgggcggctt
      121 gaggcagaca ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg tctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006503
VERSION     KHUT01006503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggtgcgca ggcgggatgg
       61 caagttggat gtgaaaactc cgggctcaac ccggagcctg cattcaaaac tgctgttctt
      121 gagtgaagta gaggcaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcctgctggg cttttactga cgctgaggca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006504
VERSION     KHUT01006504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           82 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatta ctgggcttaa agggtccgca ggcggaccag
       61 caagtacctt gtgaaatctc aggtctcaac cctgagtctg ctaggtagac tgctggtctt
      121 gagtgcaaaa ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggt ttgcaactga cgctcatgga cgaaagcgtg
      241 gggatcaaac ggg
//
LOCUS       KHUT01006505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006505
VERSION     KHUT01006505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcttt
       61 taagtcagcg gtgaaatctc cgggctcaac ccggaaattg ccgttgatac tattagtctt
      121 gaattaagtt gaggtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgctaaa cttatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006506
VERSION     KHUT01006506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           88 g           45 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 caagtcagat gtgaaagccc ggggctcaac cccggaactg catccgatac tgccgtgcta
      121 gagtactgaa ggggtgacta gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaataccaaa ggcgaaggca ggtcactggg cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006507             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006507
VERSION     KHUT01006507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttggg
       61 gaagtcgggc gtgaaatacc ggggctcaac tccgggacgc cgtccgatac tcccctgact
      121 agagtccggt aggggaaggt ggaatacctg gtgtagcggt gaaatgcaca gatatcaggt
      181 ggaacaccgg tggcgaaggc ggccttctgg gccggtactg acgctgagga gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006508
VERSION     KHUT01006508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcctgt
       61 taagttggtt gtgaaaaccc acggctcaac cgtggaagtg cgtccaaaac tggcaggctg
      121 gagtcccgaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gccatctggg agggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006509
VERSION     KHUT01006509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           87 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggccctg
       61 taagttagag gtgaaagccc ggagctcaac ttcggaattg cctttaagac tgcagagcta
      121 gaatcgtgga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactgga cacgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006510
VERSION     KHUT01006510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           96 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 taagtccgct gtgaaagccc gaggctcaac ctcgggatgc cagtggaaac tgtcgggctt
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006511
VERSION     KHUT01006511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcagag gtgaaatccc aaggctcaac cttggaactg cctttgatac tgggagtctt
      121 gaggtcgaga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcgattctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006512
VERSION     KHUT01006512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           44 c          100 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatgtt
       61 ttagtcaggt gtgaaattcc tgggcttaac ctgggggctg catttgatac ggggcatctg
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006513
VERSION     KHUT01006513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           98 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcacct
       61 taagtacaat gtgaaatctt cctgctcaac gggaagggtg catagtaaac tgaggagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006514
VERSION     KHUT01006514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggctgat
       61 caagtctgct gtcaaagcgt ggagcttaac tccatatcgg cggtggaaac tgatgagcta
      121 gagtgcgata ggggtagcag gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccagc ggcgaaagcg tgctactggg tctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01006515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006515
VERSION     KHUT01006515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtctcg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaagtg cactcgaaac tgcaaggctt
      121 gagtatggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006516
VERSION     KHUT01006516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           76 g           59 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagctc ccagctcaac tgggaaactg ccattgatac tgatagtctt
      121 gagtatgatt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006517
VERSION     KHUT01006517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          102 g           40 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatta ctgggcgtaa agagcgcgta ggtggcgctg
       61 tacgactggt gtgaaagccc ccggctcaac tggggagggt catcggtaac ggcagtgctt
      121 gagtgcagga gagggacgtg gaactccggg agtagcggtg aaatgcgtag agacccggag
      181 gaacaccagt ggcgaaggcg gcgtcctggt ctgcaactga cgctgtggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006518
VERSION     KHUT01006518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           78 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttttg
       61 taagtcagtg tttaaagtca gcagcttaac tgttgtaatg gcattgatac tgcagaactt
      121 gagtaaagtt gaggttggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gctaactaag ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006519
VERSION     KHUT01006519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           82 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttta
       61 taagtcagtg gtgaaatagt gcagcttaac tgtagaattg ccattgatac tgtagagctt
      121 gagtacagat gaggtaggcg gaattgatgg tgtagcggtg aaatgcatag ataccatcaa
      181 gaacaccgat tgcgaaggca gcttactaag ctgttactga cgctgaggca cgaaagtgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01006520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006520
VERSION     KHUT01006520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacagaggct ccaagcgtta ggcggaatca ctgggcttaa agcgtgcgta ggcggccttt
       61 caagtgtctt gtgaaagccc acggctcaac cgtggaattg ctgggcatac tggaaggctt
      121 gaattaccta ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccgag ggcgaaagca gctcgctgga ggtatattga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006521
VERSION     KHUT01006521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           90 g           47 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agagcgtgta ggcgggtaag
       61 taagtcagcc gtgaaagctc ccggctaaac tgggagaggt cggatgatac tgctaatctt
      121 gaggacaata gaggggtgta gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccaga ggcgaaggcg gcaccctgga ttgtaactga cgctgagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006522
VERSION     KHUT01006522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           84 g           61 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggagcgta gacgggtcct
       61 taagtcagtt gtgaaagttt gcggctcaac cgtaaaattg cagttgatac tggggacctt
      121 gagtgcggca gaggcaggcg gaattcgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gcttgctgga ccgtaactga cgttgatgct cgaaagtgcg
      241 ggtatcaaac agg
//
LOCUS       KHUT01006523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006523
VERSION     KHUT01006523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcacgca ggcggcttga
       61 caagttgggg gtgaaatccc ggggcttaac cccggaactg ccttcaaaac tgtcaagcta
      121 gagatcgaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgaatctga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006524
VERSION     KHUT01006524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           92 g           42 t
ORIGIN      
        1 tacgaactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgat
       61 caggtccgat gtgaaatccc acggctcaac cgtggaatgg cattggaaac cgaacggctc
      121 gagtgagcta gagggacacg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg gtgtcctggg gcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006525
VERSION     KHUT01006525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggtggcttgg
       61 taagcgtgtg gtgaaagtcc ggggctcaac cccggatctg ccgtgcgaac tgctaggctt
      121 gagcaatgta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccagt ggcgaaggcg gcctgctggg catttgctga cactgaggca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006526
VERSION     KHUT01006526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           92 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttgt
       61 taagtccgat gtgaaagccc tgggctcaac ctgggaatgg cattggaaac tggcgagcta
      121 gagtaaggta gaggagtgcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacatccgt ggcgaaggcg gcactctgga ccattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006527
VERSION     KHUT01006527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgagg
       61 taagtcagat gtgaaattcc ggggctcaac cccggaactg catccgatac tgccttgcta
      121 gagtagtgga ggggcatctg gaattcacgg tgtagcagtg aaatgcgtag agatcgtgag
      181 gaacactagt ggcgaaggcg agatgctgga cacttactga cgctgaggct cgaaggccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01006528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006528
VERSION     KHUT01006528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggttcg
       61 tgtgtcgatt gtgaaatccc tgggctcaac ctaggaattg cagtcgaaac tgcggatctt
      121 gaatacctga gagggtgggg gaattgctgg tggagaagtg aaattcgtag atatcagcag
      181 gaacacctga ggcgaaggcg cctacctggc agtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006529
VERSION     KHUT01006529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 cacgtatggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggctcgc
       61 taagcctggt gtgaaaggct ccggctcaac cggagacacg cgctgggaac tggcgggctg
      121 gagttcagga gaggaagcta gaattcccgg tgtaggggtg aaatctgtag atatcgggaa
      181 gaataccggt ggcgaaggcg agcttctggc catgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006530
VERSION     KHUT01006530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccttt
       61 caagtcaggt gtgaaatccc ccggctcaac cggggaatgg cgcttgaaac tggagggctt
      121 gagtcagcta gaggtaggcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaagcg gcctactgga gctggtctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006531
VERSION     KHUT01006531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           96 g           40 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggaccag
       61 taagcgtccc gtgaaatccc ccggctcaac cggggaactg cggggcgaac tgctggtcta
      121 gaggaagcta gaggcgggta gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaatgccgaa agtgaagaca gcccgctggg gctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006532
VERSION     KHUT01006532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           48 c           73 g           57 t
ORIGIN      
        1 tcccagcagt ccaagtcgca atcacaatta ttgggtctaa aacatccgta gcttgcttcg
       61 taagtctctt gtgaaatccg gcctcttaag ggtcgaagtg cgagagatac tgcgttgcta
      121 gagaccggga gaggtaagaa gtacaaatgg agtagcggta aaatgtgttg atcctatttg
      181 gactaacaac agcgaaggca tcttactaga acggatctga cagtgaggga tgaaggctag
      241 gggcgcaaaa ggg
//
LOCUS       KHUT01006533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006533
VERSION     KHUT01006533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           95 g           52 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttcgt
       61 taagtcaggt gtgaaatccc tcggctcaac cggggaattg cgcttgaaac tagcgggctg
      121 gagcgaggta ggggtatgtg gaacttccgg tggagcggtg aaatgtgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcatactggg ccttgtctga cgctgaggca cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01006534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006534
VERSION     KHUT01006534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggattca ttgggcttaa agcgtacgta ggcggatgtg
       61 caggcatcgt gtgaaatccc ctggctcaac cagggaactg ctcggtgaac cgcacgtctt
      121 gaggcaagta gaggctgtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccgaa ggtgaaaaca ggcagctggg cttgttctga cgctaaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006535
VERSION     KHUT01006535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgac
       61 gaggtcaggt gtgaaatccc ccggctcaac cggggacgtg cgcttgaaac cggtcggctc
      121 gagtgagcta gaggcatgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgtaagcg gcatgctggg gcttgactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006536
VERSION     KHUT01006536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c          101 g           39 t
ORIGIN      
        1 aacgtagggg tcgagcgtta tccggaatta ctgggcgtaa agcgcgtgca ggcggcgtgg
       61 caagtgggac gtgaaagctc ctggctcaac tgggagagga cgttcgagac tgccaggctc
      121 gagaacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctggg ccggttctga cgctcagacg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01006537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006537
VERSION     KHUT01006537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           84 g           54 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 aaagttgggt gtgaaatccc ttggcttaac caaggaattg cgctcaaaac tactgtgcta
      121 gaggaataca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg tattttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006538
VERSION     KHUT01006538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          102 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcga
       61 gcagtcggtc gtgaaagtcc ggagctcaac tccggatcgg cggtcgatac tctcgtgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006539
VERSION     KHUT01006539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtcccg
       61 caagtgagat gtgaaatccc cgggcttaac ctgggaactg catcttaaac tgcggggctg
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006540
VERSION     KHUT01006540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtcgggg gtgaaatctt tcggcccaac cggaaaaggg cctccgatac tggcagcctc
      121 gaggggggta ggggtcggcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gccgactgga cctcacctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006541
VERSION     KHUT01006541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcgggtcgt
       61 taagtcgagg gtgaaatcct ggagctcaac tccagaactg cccccgatac tggcgacctc
      121 gagtccggaa gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgatatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006542
VERSION     KHUT01006542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcag
       61 caagtccggg gtgaaatcct ccggcttaac cggagaagtg ccttggatac tgctggtctc
      121 gagggaggta ggggtcatgg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg catgactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006543
VERSION     KHUT01006543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttag
       61 taagtcggtc gtgaaagccc tgggcttaac ctaggaatgg cgatcgaaac tgttagacta
      121 gagtatcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006544
VERSION     KHUT01006544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtttt
       61 caagtccggg gtgaaatcct ctcgctcaac gagagaactg ccttggatac tgaggacctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006545
VERSION     KHUT01006545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          104 g           39 t
ORIGIN      
        1 gacggagggt gcgagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggcttct
       61 taagtccggc gtgaaagccc agggctcagc cgtggaaggg cgccggaaac tgggaggcta
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006546
VERSION     KHUT01006546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggtcttg
       61 taagtcgggc gtgaaatctc accgcttaac ggtgaaactg cgttcgatac tgcttggcta
      121 gaggatcgga gggggtatcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccggt ggcgaaagcg gatacctgga agattcctga ctctgaaaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006547
VERSION     KHUT01006547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           88 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctatt
       61 caagtcagag gtgaaagccc ggggctcaac cccggaatag cctttgaaac tagatagcta
      121 gaatctggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccagaattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006548
VERSION     KHUT01006548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          104 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggtccct
       61 taagtccggg gtgaaaggcg gaggctcaac ctccgagctg cgccggaaac tgggggtctt
      121 gaatgtcgga gaggggcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcgccctggc cgaacattga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006549
VERSION     KHUT01006549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           86 g           54 t
ORIGIN      
        1 tacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggtggttgga
       61 taagtctgtt atgaaatctc ccggcttaac tgggagcggt taatggatac tgtccggctc
      121 gagtgtggga gaggttactg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaat ggcgaaagca ggtaactggc ccaatactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006550
VERSION     KHUT01006550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           64 c          107 g           31 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggcaccg
       61 tatgtgcatg gtgacatccc acggctcaac cgtgggcggc ccaagtacac agcggcgctg
      121 gaggcaggga gagggtcgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcgacctggc cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006551
VERSION     KHUT01006551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           90 g           43 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggccccg
       61 aaagcgtcgt gtgaaagccc ctggctcaac cagggaacag cacggcgaac tacggggctt
      121 gagaaaacta ggggccgaca gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggtgaagacg gtcggctggg gtttttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006552
VERSION     KHUT01006552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c          100 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggtctgg
       61 taagtcgggg gtgaaaaccc agagctcaac tctgggagtg cctctgaaac tgctggactt
      121 gagtgctcta gagggtagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg actgcctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006553
VERSION     KHUT01006553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c          102 g           44 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttagg
       61 taagtcctgt gggaaagcct agggcttaac cctggaaggt cgcaggagac tgcctagcta
      121 gaggacggaa gaggagggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gctctctgga acgttcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006554
VERSION     KHUT01006554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacgaaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggtgggcttg
       61 taagtcggat gtgaaagccc caggcttaac ctgggaatgg cacccgaaac tataagtctt
      121 gaatgcttga gaggttggtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaatatcgga ggcgaaggcg gccaactggt ggtgcattga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006555
VERSION     KHUT01006555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggattta ctgggcgtaa agggtgcgta ggcggtttaa
       61 taagttaagt gtgaaagtcc tgggcttaac ctggggaagt cacttaagac tgttagactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006556
VERSION     KHUT01006556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgagg
       61 taagtcgggt gtgaaatcct ggggctcaac cccagaactg cattcgatac tgctttgctc
      121 gagtactgga gaggagattg gaatttatgg tgtagcagtg aaatgcgtag atatcataag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006557
VERSION     KHUT01006557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggttacg
       61 cgagtcaggt gtgaaagccc agggctcaac cctggaagtg cacttgatac tacgtagctg
      121 gagtcctgta gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caggtactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006558
VERSION     KHUT01006558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtcaaa
       61 taagtctggt gtgaaagccc tgggctcaac cgaggaagtg catcagaaac tatttgacta
      121 gaagacggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctggg ccgatcttga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006559
VERSION     KHUT01006559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggctgag
       61 aaagtagggt gtgaaatccc acggcttaac cgtggaactg cgccctaaac tactcagctt
      121 gagggagcta gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gcttactggg gcttttctga cgctgaggga cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006560             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006560
VERSION     KHUT01006560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           85 g           49 t
ORIGIN      
        1 tacagaggga gcaagcgtta tccggaatga ttgggcgtaa agcgtttgta ggtggttcgt
       61 aaagtttaat gtcaaatatc agggctcaac cctgaaccgg cattgaagca ctcacgaact
      121 tgagtacggt agaggcagag ggaattcctt gtggagcggt gaaatgcgta gagataagga
      181 ggaacaccag tggcgaaggc gctctgctgg gccgaaactg acactgagaa acgaaagctg
      241 ggggagcgaa tagg
//
LOCUS       KHUT01006561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006561
VERSION     KHUT01006561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           85 g           55 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggcggacctc
       61 taagtcaggt gtgaaatccc acggctcaac cgtggaattg catttgaaac tggaggtctt
      121 gagtgtggga gagattggta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccggt ggcgaaggcg gccaattggc ccaacactga cgctgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006562
VERSION     KHUT01006562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           86 g           56 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ctgggtgtaa agggcgcgta ggcgggttcg
       61 taagtcagag gtgaaatctc ccggcttaac cgggaaactg cctttgatac tgcgaatctt
      121 gagttcagaa gagagagatg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt ggcgaaggcg atctcttggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006563
VERSION     KHUT01006563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggaatta ctgggcgtaa agagcacgta ggcggttatg
       61 taagtcggat gtgaaagccc agggcttaac cttggaattg cattcgaaac tgcatggcta
      121 gagtttggta gaggctagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gctggctgga ccaaaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006564
VERSION     KHUT01006564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           90 g           52 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggaatta ctgggcttaa agggtgcgta ggcggtcccg
       61 taagtgcctt gtgaaatccc atggctcaac catggaattg ctcggtagac tgcgggactt
      121 gaggtcggta gaggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgtagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006565
VERSION     KHUT01006565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttatt
       61 taagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgagac tggataacta
      121 gaatcttgga gaggcgggtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcccgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006566
VERSION     KHUT01006566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           90 g           46 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tggcgatctt
      121 gagccaccta ggggcgagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaagaca gctcgctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006567
VERSION     KHUT01006567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           87 g           57 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttagt
       61 taagtcagat gtgaaatccc tgggctcaac cgaggaagtg catttgaaac tatctagcta
      121 gagaatggta gaggaaggtg gaattccgtg tgtagaggtg aaattcgtag atatacggag
      181 gaataccggt ggcgaaggcg gccttctgga ccatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006568
VERSION     KHUT01006568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggagtgta ggctgtctgg
       61 aaagtcttct gtgaaatcta cgggcttacc ctgtaaagtg cagaggaaac ttccaggctt
      121 gagtgtcgga gaggacaggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006569
VERSION     KHUT01006569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccctc
       61 cacgtcggat gtgaaagctc ggagctcaac tctgagaggc cattcgatac gggaaggcta
      121 gaggtaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006570
VERSION     KHUT01006570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacgtatggg gcaagcgtta tccggattca ttgggcgtaa agagcgcgta ggtggcttgt
       61 taagcgagat gtgaaaaccg ggggctcaac ccgcggcctg catctcgaac tggcaggctt
      121 gagtctggta gaggagtgtg gaattcctag tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccagt ggcgaaggcg gcactctggg ccagcactga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006571             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006571
VERSION     KHUT01006571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           48 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcggat gtgaaaactc caggctcaac ctggagacgc cattcgatac tgctgtggct
      121 agagtccagt aggggagtgt ggaattcctg gtgtagcggt gaaatgcata gatatcagga
      181 ggaacaccag cggcgaaggc ggcactctgg gctggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006572             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006572
VERSION     KHUT01006572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           94 g           51 t
ORIGIN      
        1 aacggggggg gcgggtgttg ctcaccatta ctgggcgtaa agggcaagta ggcggtctgc
       61 cgtctgctgg attaaagccc acggctcaac cgtggttctg ttcagcatag tggcagtctt
      121 gagttcggta gaggttgaca gtattgccgg agtagaggtg gcattcggtg ataccggcaa
      181 gactcccaat aggcgaaggc ggtcgactgg tccgatactg acgctgagag gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006573             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006573
VERSION     KHUT01006573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcgggt gtgaaatctc caggctcaac ctggagaggt cacccgagac tgccatgact
      121 agagtccggt aggggtgtgt ggaattcctg gtggagcggt gaaatgcgca gatatcagga
      181 ggaacacccg tagcgaaggc ggcacactgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006574             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006574
VERSION     KHUT01006574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c           98 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggttctt
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccggatac tgaagggctt
      121 cgagtgcggg aggggagagt ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacacctg tggtgcaaac ggctctctgg accgtaactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006575
VERSION     KHUT01006575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           84 g           56 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtatgta ggcggctttt
       61 caagtcagat gtgaaagccc acggcttaac cgtggaaatg catttgaaac tgctaagctt
      121 gagtgcgaaa gaggatggta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccgga ggcgaaggcg gccatctggt tcgctactga cgctgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006576
VERSION     KHUT01006576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggctgaccgg
       61 gaagtcaggt gtgaaatccc tgggctcaac ccaggaactg catttgaaac ttccggtctt
      121 gagggatgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga catcttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006577
VERSION     KHUT01006577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggatact
       61 taagtcaggg gtgaaagccc gaggctcaac ctcggaactg cctttgatac tgagtatctt
      121 gagaccggta gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006578             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006578
VERSION     KHUT01006578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           76 g           62 t
ORIGIN      
        1 tacagaggtc acaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggtgggttt
       61 ataagttatt tttcaaatcc caaggctcaa ccttggaaag ggagataata ctgtaagtct
      121 tgactactgg tggggtatct ggaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaactccaa gggcgaaggc aggatactaa ccaggcagtg acactgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01006579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006579
VERSION     KHUT01006579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          106 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggccgtg
       61 tatgtgctgg gtgacagctt cccgcttaac gggaagtggc cccggtagac agcagggctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006580
VERSION     KHUT01006580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           37 c           85 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcttgta ggtggttata
       61 taagttagat gtgaaaaccc tgggctcaac ctgggacgtg catttaatac tgtatgacta
      121 gagtatagta gaggaaaggg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacatcggt ggcgaaggcg cctttctgga ctaatactga cactgaggag cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006581
VERSION     KHUT01006581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 gacgtagggg gccagcgttg ctcggaatta ctgggtgtaa agggttcgta ggcggtgtgg
       61 caagtcggga gtgaaatctc tgagcttaac tcagggcctg cttccgaaac tgctgtgcta
      121 gagtgtggga gaggcgcgtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaagcg gcgcgctgga ccactactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006582             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006582
VERSION     KHUT01006582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           93 g           52 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggttcgg
       61 taagtcgggt gtgaaaactc caagctcaac ttggagcctg cattcgaaac tgctgtgact
      121 cgagtcaggt aggggaacac ggaattccag gtgtagcggt gaaatgctta gatatctgga
      181 ggaacaccag tggcgaaggc ggtgttctgg gcctgtactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006583
VERSION     KHUT01006583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 tacagggggc tcgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggctcgc
       61 caagtgcttt gtgaaatccc acagctcaac tgtggaacag cagggcaaac tggcgagctt
      121 gaggcaggta ggggccggaa gaacagttgg tggagcggtg aaatgcgtag atatcaactg
      181 gaatgccgat ggggaagccg tccggctggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006584
VERSION     KHUT01006584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           96 g           49 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgtgg
       61 taagtctgtt gtgaaacctc caggctcaac ttggagcttg catcagaaac tgccgtgcta
      121 gagtgtggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctctctgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006585
VERSION     KHUT01006585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           87 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgg
       61 taagttaagt gtgaaagccc cgggcttaac ctgggaattg cgcttaatac tgcctgactt
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006586
VERSION     KHUT01006586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          102 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agggtccgta ggcggtgctt
       61 tcagttcctg ctgacagtcc tcagcttacc tgaggaaggt gtaggaagac gggggcactt
      121 gagggcggca gagggagacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgtaggca gtctcctggg ccgccactga cgctgaggga cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006587
VERSION     KHUT01006587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           91 g           53 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattca ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtctgat gtaaaaggct cgggcccaac ccgagtatcg cattggatac tggcagactg
      121 gagtgcagta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaggcg acttgctggg ctgtaactga cgctgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01006588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006588
VERSION     KHUT01006588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           80 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggcctgg
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cctttgatac tgccgggctt
      121 gaataaacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactatg gttttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006589
VERSION     KHUT01006589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggctgtgccg
       61 caagtcaggg gtgaaatccc agagctcaac tctggaactg cctttgaaac tgcggtgctg
      121 gaatctcaga gggggtagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctacctgga tgagtattga cgctgaggta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006590
VERSION     KHUT01006590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           85 g           53 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagtgc
       61 taagtacctt gtgaaatccc acagctcaac tgtggaactg cttggtatac tggttctctt
      121 gagccactta ggggcaatcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gattgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006591
VERSION     KHUT01006591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           96 g           41 t
ORIGIN      
        1 aacgtaggac acgagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtcgaa
       61 taagtctgat gtgaaagctc ccggctcaac cgggagagga cattggagac tgctcgacta
      121 gaggcagata gaggggtgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcaccctggg tctgacctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006592
VERSION     KHUT01006592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtcacg
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgcgcgactt
      121 gagtacggga gagggttgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcagcctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006593
VERSION     KHUT01006593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           84 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gtcggctgtt
       61 taagtcgggt gtgaaatctc cgggctcaac ctggaaattg cattcgatac tggacagcta
      121 gagttcggca gagggaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006594
VERSION     KHUT01006594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatta ctgggcgtaa agggagcgta ggcggcgggg
       61 taagtcagat gtgaaatacc ggggctcaac tccgggacgg catccgatac tgccttgcta
      121 gagtagtgga ggggcgactg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacactagt ggcgaaggcg agtcgctgga cactaactga cgctgaggct cgaaggccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01006595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006595
VERSION     KHUT01006595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           97 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgat
       61 tgggtgtgat gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac tggtgaactg
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006596
VERSION     KHUT01006596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatta ctgggcgtaa agggcgagta ggtggctcga
       61 taagtaggag gtgaaatctc acggctcaac cgggaggctg ccttctagac tgtcgagctc
      121 gagcatggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccatagctga cactgaatcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006597
VERSION     KHUT01006597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 gacgaaccga gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctat
       61 caagtccggg gtgaaatcct ccagcttaac tggagaactg cctcggatac tgatggtctc
      121 gagggagata ggggcaagcg gaactgtggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaggcg gcttgctgga tctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006598
VERSION     KHUT01006598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtccgt
       61 cacgtcggct gtgaaaaccc agggctcaac cctgggcctg cagtcgatac gggcggacta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006599
VERSION     KHUT01006599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           86 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ctgggtgtaa agggtgcgta ggcggttatg
       61 caagtcagat gtgaaatgtc gaggctcaac ttcggaactg catttgaaac tgtgtgacta
      121 gagtacagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ctgaaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006600
VERSION     KHUT01006600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           80 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta tccggaatca ctgggcgtaa agggcgtgta ggtggtttgt
       61 taagttcgat gttaaatact gaagctcaac ttcagcacgg cgttgaatac tggcaggcta
      121 gacaattgga gaggcaagta gaattaccag tgtagcggtg aaatgcgtag atactggtag
      181 gaatacccat tgcgaaggca gcttgctaga caatttgtga cactcagacg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006601
VERSION     KHUT01006601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgagg
       61 taagtcggat gtgaaatctc ggggctcaac cccgaaactg cattcgatac tgctttgctt
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006602             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006602
VERSION     KHUT01006602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          104 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcttct
       61 taagtctatg ggtgaaagac cggggctcaa ccccgtgtca gcccgggata ctgggaagct
      121 agggacatgt aggggcgagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg gggcgaaggc ggctcgctgg gcatggtccg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006603             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006603
VERSION     KHUT01006603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           84 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggatgtt
       61 taagtgtgat gcgaaatcct gtggctcaac catatggact gtatcgcata ctggatatct
      121 ggaggacatg agaggcaagt ggaattaccg gtgtagcagt aaaatgcgta gagatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg catgtttctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006604             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006604
VERSION     KHUT01006604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           96 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccag
       61 ctaagtcgag tgttaaatcc cagagcttaa ctctggacag tcgctcgata ctagcgggct
      121 agagtgtgga agagggtgct ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc gggcacctgg gccaacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006605
VERSION     KHUT01006605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           63 c          113 g           33 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggcgcgg
       61 tgagcccggc gtgaaagccc ccggcctaac cggggaaggg cgtcggggac tgccgcgctt
      121 gagggcagca gggggtggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccacctggg ctgcgcctga cgctgaggtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006606
VERSION     KHUT01006606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatca ctgggcgtaa agggcgtgca ggcggcccgg
       61 taagtggaat gtgaaatgcc ctggctcaac cagggaactg cattccagac tgccaggctt
      121 gagtacagga gaggatgggg gaattcccgg tgtagcggtg gaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggc ctgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006607
VERSION     KHUT01006607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggtttga
       61 taagtctatt gtgaaagctc ccggcttaac tgggagaggc caatggaaac tgtcaggcta
      121 gagtgtggta gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca gtcttctgga ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006608
VERSION     KHUT01006608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           98 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggcttat
       61 tgagttaggc gtgaaaggcc tgggcttaac ctgggaactg cgcttaatac ggataggctt
      121 gagtccggaa gggggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccacctggt ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006609
VERSION     KHUT01006609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacggggggt gcaagcgtta ttcggaatca ttgggcgtaa agagcacgta ggcggcctgg
       61 taagtcagat gtgaaagccc ggggcttaac cccggaagtg catttgatac tgtcaggctt
      121 gagtatggga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctgga ccaatactga cgctgaggtg cgaaggcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006610
VERSION     KHUT01006610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           39 c           84 g           63 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatta ctgggtttaa agggtgcgta ggcgggagat
       61 taagtcagtg gtgaaagcct gtcgcttaac gatagaattg ccattgatac tgattttctt
      121 gaattgggtt gaggttagcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcatg
      181 gaacaccaat tgcgaaggca gctaactggg ccttgattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006611
VERSION     KHUT01006611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 cacgaacccg acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggtgcgg
       61 taagtgaggt gtgaaagccc tcggctcaac cgaggaattg cgcttcatac tgccgtgcta
      121 gaggaagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttctctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006612
VERSION     KHUT01006612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacgtaggtg tcaagcgtta tccggaatta ctgggcgtaa agggcacgca ggcggcctgg
       61 gaagtccgac gtgaaagctc ccggcttaac tgggagaggt cgttggaaac tttcaggctt
      121 gagagagtga gagggaggcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gcctcctggc actgttctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006613
VERSION     KHUT01006613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacatt
       61 taagtcgggg gtgaaagccc ggggctcaac cccggaattg cctccgatac tggatgtctc
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006614
VERSION     KHUT01006614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           86 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggctgtgcgg
       61 taagtcagtg gtgaaatccc agagcttaac tctggaactg ccattgaaac taccgcgcta
      121 gaatctcaaa ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctggg tgagtattga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006615
VERSION     KHUT01006615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctgg
       61 taagtcggcg atgaaagccc ggggctcaac cccggaactg tcgtcgatac tgtcggactt
      121 gagtacgggt gaggctggcg gaatgggcgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctggctaag ccgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006616
VERSION     KHUT01006616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 gacgtaggaa gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggatcg
       61 taagtcttag agtaaatctc acggctcaac cgtggggcct tctgagatac tgcgattctt
      121 gaggatgtca gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggg acattcctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006617
VERSION     KHUT01006617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           77 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcagtt
       61 taagtcagag gtgaaagacg gtagctcaac tatcgcagtg cctttgatac tgaattgctt
      121 gaataaacta gaggtaggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gcttactatg gttttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006618
VERSION     KHUT01006618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggtcat
       61 taagtcagtg gtgaaatccc ggagctcaac tccggaactg ccaatgatac tattgatctt
      121 gaatcctgtt gaggtgggcg gaatacatca tgtagcggtg aaatgcttag agatgatgta
      181 gaacaccgat tgcgaaggca gctcactaag caggtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006619
VERSION     KHUT01006619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           81 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgca ggcgggctat
       61 taagtccgtg gtgaaatccc ggggctcaac cccggaactg ccatggatac tattagtctt
      121 gaatgttgtt gaggtgggcg gaataggtca tgtagcggtg aaatgcatag atatgaccta
      181 gaacaccgat tgcgaaggca gctcactgga caaatattga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006620
VERSION     KHUT01006620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgg
       61 caagtctgac gtgaaatccc gcggctcaac cgcggtaggt cgttggaaac tgcctcgcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006621
VERSION     KHUT01006621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           89 g           44 t
ORIGIN      
        1 tacgtagagg gcgagcgtta tccggaatta ttgggcgtaa agggtgtgca ggcggcttac
       61 caagttcgtg gcgacagcta tcggcttaac cgatggagga ctgcgaaaac tggtaaactt
      121 gagagcgaga gagggacatg gaactccggg tggagcggtg acatgcgtag agatccggaa
      181 gaacaccaat ggcgaaggca gtgtcctagc tcgtatctga cgctcaagca cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01006622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006622
VERSION     KHUT01006622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ttgggcgtaa agggtgcgca ggcggtttgc
       61 caagttcgtg gcgacagcta tcggcttaac cgatggagga ctgcgaaaac tggcagactt
      121 gagagcgaga gagggacatg gaactccggg tggagcagtg aaatgcgtag agatccggaa
      181 gaacaccaac ggcgaaggca gtgtcctggc tcgtctctga cgctcaagca cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01006623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006623
VERSION     KHUT01006623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccag
       61 caagtcaggg gtgaaatctt tcagctcaac tggaaaaggg cctttgatac tgttggtctc
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006624
VERSION     KHUT01006624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaacag cctctgatac tggtggtctg
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006625
VERSION     KHUT01006625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agggtttgta ggcggccttg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaatgg cgcttgatac tgcacggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006626
VERSION     KHUT01006626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgga
       61 caagtcgagt gtgaaatccc cgggctcaac ctgggagctg cattcgaaac tgttcggcta
      121 gagtatggga gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006627
VERSION     KHUT01006627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          100 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctcgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tggcgagctt
      121 gagcagagta gaggcgggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctcttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006628             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006628
VERSION     KHUT01006628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           92 g           54 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ttgggcttaa agagcacgta ggcggcgaca
       61 taagtcggag gtgaaatact gtcggctcaa ccgcggaatt gcctttgata ctgtgttgct
      121 tgaggggttt aggggcgagt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccag tggcgaaagc gactcgctgg gaattttctg acgctgaggt gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006629
VERSION     KHUT01006629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           98 g           48 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgcgca ggcggcttcg
       61 tgagtcggat gtgaaagctc ctggcttaac tgggagaggt cattcgaaac tgcggggctt
      121 gaggttggga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006630
VERSION     KHUT01006630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           85 g           55 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agagttcgta ggtggcttta
       61 taagtcgggt gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcaaagctt
      121 gaatatggaa gagggtagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gctacctggt ccaatattga cgctgaggaa cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01006631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006631
VERSION     KHUT01006631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggactg
       61 taagtcagat gtgaaattcc aaggcttaac cttggcgctg catctgaaac tatggttctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006632
VERSION     KHUT01006632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           84 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggccgcgcgg
       61 gcagtcaaat gtgtaattca gaggctcaac ctctggactg cgcttgatac ttccgtgcta
      121 gaaccttgga gagggatctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacactcag ggcgtaagca agatcctgga caagtgttga cgctcatgca cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006633
VERSION     KHUT01006633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaatg
       61 caagtcagat gtgaaatgcc gaggcttaac ctcggagctg catctgaaac tgtgtttctt
      121 gagtgtcgga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006634
VERSION     KHUT01006634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa aggatgcgta ggtggttctt
       61 taagtgggat gtgaaatccc tgggcttaac ccaggaattg cattccaaac tggaggacta
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cactgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006635
VERSION     KHUT01006635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggtaccc
       61 taagtcagat gtgaaaaccc ggggcttaac tccggacgtg catttgaaac tggggaactt
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006636
VERSION     KHUT01006636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           64 c          101 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 ccagtcggag gtgaaagccc ccagctcaac tggggaagcg ccctcgatac tgccggcctt
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gcacgctggg cctctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006637
VERSION     KHUT01006637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 gacggaggag gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcttg
       61 caagtcaggg gtgaaagccc ggggctcaac ctcggaactg cctttgagac tgtgagactc
      121 gagaccgaga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggc tcggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006638
VERSION     KHUT01006638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 cacgtaggag acgagcgtta tccggattta ctgggtgtaa agcgcgtgta ggcggtcgag
       61 taagttggat gtgaaagctc ccggctcaac tgggagaggt cgttcaatac tgcttggcta
      121 gaggacagca gaggggagtg gaattccgag tgtagcggta aaatgcatag atattcggaa
      181 gaacaccagt ggcgaaggcg gctctctggg ctggtcctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006639             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006639
VERSION     KHUT01006639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          102 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcgggcggc
       61 acagtcgggc gtgaaattcc tgggcttaac ctgggggctg cgtccgagac gtgttgccta
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttttactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006640             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006640
VERSION     KHUT01006640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctaag
       61 caagtcgggt gggaaatctc caggctcaac ctggagcctt cgctcgatac tgctttagct
      121 cgagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccga cggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006641             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006641
VERSION     KHUT01006641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttggg
       61 taagtcgggt gtgaaaactc agggctcaac tctgagacgc cactcgatac tgctctgact
      121 cgagtccggt aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006642
VERSION     KHUT01006642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcctat
       61 taagtctgac gtgaaatctc tcggcttaac cgagagaggt cgttggaaac taactggctt
      121 gaggcaatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcaccctggc atttgcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006643
VERSION     KHUT01006643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gatggttcac
       61 tcagtcggat gtgaaagccc cgggctcaac ctgggaactg catccgatac tggtggacta
      121 gagtgcggta gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga ccagcactga cgttgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006644             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006644
VERSION     KHUT01006644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c          100 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggcttct
       61 taagtctggg gtgaaaggtt caacgctcaa cgtgaacagt gccttggata ctggggagct
      121 tgagttcggg agggggcagt ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccga tggcgaaggc agctgcctgg cctagaactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006645
VERSION     KHUT01006645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           94 g           42 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcggc
       61 cgcgtcagat gtgaaagccc cgggctcaac ctgggaaccg catttgaaac gggtcgactc
      121 gagttcggga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgag ggcgaaggca gctatctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006646             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006646
VERSION     KHUT01006646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           43 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatca ttgggcgtaa agggctcgta ggcggaccgg
       61 tcagtctggc gtgaaatctc caccactcaa cggggagagg tcgtcggata ctgccggcct
      121 ggagtacggt agaggaccat ggaattcctg gtgtagcggt ggaatgcgta gagatcagga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgttactg acgctgagga gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006647             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006647
VERSION     KHUT01006647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           66 c          100 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgg
       61 cacgtccgtc gctgaaatcc cccggctcaa ccggggaagt ggcgtggata cgaccgggct
      121 ggaggggggt agggggagcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggctcctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006648             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006648
VERSION     KHUT01006648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           63 c          111 g           34 t
ORIGIN      
        1 tacggagggc gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccctg
       61 gtaagtctgc ggtgaaagcc cggggctcaa ccccgggtcg gccgcgggta ctgccgggct
      121 ggagtgccgt aggggcaggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcctgctgg gcggcgactg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006649
VERSION     KHUT01006649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           87 g           51 t
ORIGIN      
        1 tacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agggcaggta ggcggtttga
       61 taagtctatt gtgaaagctc ccggcttaac cgggagaggt caatggatac tgttgaactt
      121 gagtatggga gagggtactg gaattcctgg tgtagcggtg aaatgcgcag agatcaggag
      181 gaacaccgat ggcgaaagca ggtacctgga ccattactga cgctcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006650
VERSION     KHUT01006650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           89 g           46 t
ORIGIN      
        1 tacagagggc tcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggatgca
       61 aaggcacctt gtgaaatccc tcggctcaac cgaggaattg cttggtgaac catgcatctt
      121 gaggcaggta ggggtggctg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccaaa ggagaagtca ggccactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006651
VERSION     KHUT01006651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctccg
       61 cgagtcgggg gtgaaatctt ccagctcaac tggaaaaggg cctccgatac tgcgggactc
      121 gagggggata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tccctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006652
VERSION     KHUT01006652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcttgg
       61 taagtcggac gtgaaatccc ccagcttaac tggggaactg catccgatac tgccaggctt
      121 gagtcccgga gagggcagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctgcctgga cgggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006653
VERSION     KHUT01006653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctggg
       61 taagtcaaat gtgaaatccc gaggctcaac cacggaattg cgtttgaaac tgcctggctt
      121 gagtcccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actacctgga cgggtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006654
VERSION     KHUT01006654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatagg
       61 taagtcagtg gtgaaatcct gcagcttaac tgcagaactg ccgttgatac tatctatctt
      121 gaatgccgta gaggttagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccaat tgcgaaggca gctaactata cggtgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006655
VERSION     KHUT01006655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           47 c           97 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtcgaaa gtgaaaactg ctggcttaac cggcagcttg ctttcggtac gggcatgctt
      121 gagtgtggta ggggagtctg gaattcttgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006656
VERSION     KHUT01006656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtttgg
       61 atagtcggat gtgaaagccc cgggctcaac ccgggaagtg cattcgatac tcccaagcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006657
VERSION     KHUT01006657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcaagg
       61 catgtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tactttgctt
      121 gagtaccgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgtctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006658
VERSION     KHUT01006658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c           99 g           44 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggctcgg
       61 tcagtcggat gtgaaaacct gtggctcaac cacaggctgt cattcgaaac tgccgggctt
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01006659             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006659
VERSION     KHUT01006659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           91 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgca ggcggcccgt
       61 ttagtcagtt gtgaaatccc ccggcttaac cggcgggact gcgattgata ctggcgggct
      121 tgaatcgggt tgaggcaggc ggaatgtggc atgtagcggt gaaatgctta gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg gccttgattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006660
VERSION     KHUT01006660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacggagagg gcaagcgttg ttcggattta ctgggcataa agcgcacgta ggcggactca
       61 cgagtcggtt gtgaaatcct ggggctcaac cccagaactg cagtcgatac tatgagtcta
      121 gaggatgaaa ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggt tcatatctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006661             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006661
VERSION     KHUT01006661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           81 g           55 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggactag
       61 tcagtcgaat gtgaaatacc aaagctcaac tttggggctg catttgaaac tactagtctc
      121 agaggattgg cggagaaaat ggaattccac gtgtagcggt gaaatgcgta gatatgtgga
      181 agaacacctg tggcgaaagc gattttctag cttatacctg acgctgaggc gcgagagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006662
VERSION     KHUT01006662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           91 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agggcgcgta ggcggcgtct
       61 taagtcagat gtgaaaaccc gaggcttaac ctcgggcgtg catttgaaac tgggatgcta
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006663
VERSION     KHUT01006663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatca ctgggcgtaa agcgcgcgta ggcggctctg
       61 ccagtcaggt gtgaaagccc cgggctcaac ctgggaactg cgcttgatac tgtggagcta
      121 gagtctgtca gagggtggcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gccacctggg gcgagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006664
VERSION     KHUT01006664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgt
       61 acagtctatg gtgaaatcct ccagcttaac tggagaactg ccgtggatac tcacgacctc
      121 gaggaatgta ggggcatctg gaactggtgg tggagcggtg aaatgcgttg atatcatcag
      181 gaactccggt ggcgaaggcg aggtgctgga cattttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006665
VERSION     KHUT01006665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 caagtctggt gtgaaatctc ggggctcaac cccgaaattg caccggatac tgcgttgctc
      121 gaggattgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006666
VERSION     KHUT01006666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttt
       61 taagtcggac gtgaaatccc tgggcttaac ctaggaactg cgtccgatac tattgagctc
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006667
VERSION     KHUT01006667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgtgta ggtggttatg
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaactg cgttgaaaac tgcaagacta
      121 gagttggaca gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatccgt ggcgaaggcg gccacctggg tccaaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006668
VERSION     KHUT01006668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggct gtgaaagccc gaggcttaac ctcgggtctg cagtcgatac gggctaacta
      121 gagtgatgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cattaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006669
VERSION     KHUT01006669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcc
       61 caagtcggtg gtgaaatctt tcagctcaac tggaaaagtg ccgccgatac tgggagtctt
      121 gagggaggta ggggtcgatg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006670
VERSION     KHUT01006670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggtggtctgt
       61 taagtcatgt gtgaaatccc ccggctcaac cggggaagtg cgcatgaaac tgacaggcta
      121 gagtaccgaa gaggaaggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gccttctggt cggatactga cgctaaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006671
VERSION     KHUT01006671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 gacgagcctt gcaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc ccggctcaac cggggaatgg cgctcaaaac tactgtgcta
      121 gaggaagaca gaggtcggcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gccgactggg tcttttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006672
VERSION     KHUT01006672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           94 g           47 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggagtca ctgggcttaa agcgtacgta ggcgggcatg
       61 caggcgtcgt gtgaaatccc ccggctcaac cggggaactg cacggcgaac cgcatgtctt
      121 gagacgagta ggggctgtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggcagctggg ctcgttctga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006673
VERSION     KHUT01006673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 ccagtccggg gtgaaatctt tcggcttaac cggaaaacag ccttggatac tgatcgtctc
      121 gagggaggta ggggcctgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgggctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006674
VERSION     KHUT01006674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgtt gtgtaaactc ccggcttaac tgggagaggt caacggatac tgtcaggctt
      121 gagtatagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca ggtacctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006675
VERSION     KHUT01006675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtcagtt gtgaaaactc ggggctcaac cccgagacgt cgattgaaac cccctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006676             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006676
VERSION     KHUT01006676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           47 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcaagta ggcggttgtg
       61 taagtccgtt gttaaagccc ctggctcaac tgggggaggt caatggagac tacactgact
      121 tgagggcaac agaggcacgt ggaactcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacatccg tggcgaaggc gacgtgctgg gttgcgactg acgctgagtt gcgaaagtgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006677
VERSION     KHUT01006677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 caagacagat gtgaaatccc cgggctcaac ctgggaatgg catttgtgac tggcgagcta
      121 gagtatgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg acgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006678
VERSION     KHUT01006678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           97 g           52 t
ORIGIN      
        1 tacggaggtg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcatgg
       61 caagtcagat gtgaaagccc cgggcttaac ttgggaattg catctgaaac tgttttgctt
      121 gagtcctgga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagtgactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006679
VERSION     KHUT01006679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ttgggcgtaa agggagcgta ggcggttcga
       61 taagtcactt gtgaaatctc tgggctcaac tcagagtctg caagcgaaac tgtcgggctt
      121 gagtatggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccattactga cgctgatgct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006680
VERSION     KHUT01006680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggctgctatc
       61 taagtcgggt gtgaaatccc acggctcaac cgtggaagtg catttgaaac tggatagcta
      121 gagagtggta gaggaaggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaataccggt ggcgaaggcg gccttctggg ccacttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006681
VERSION     KHUT01006681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggtttgg
       61 tcagtcaggc gtgaaattcc cgggctcaac ctgggggctg cgcttgatac ggccagacta
      121 gaggacggaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccgttactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006682
VERSION     KHUT01006682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccgga
       61 caagtggaag gtgaaatccc cgagctcaac tcgggaactg ccctccaaac tgtttggctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006683
VERSION     KHUT01006683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           97 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgagta ggcggtccgc
       61 taagtctgat gtgaaagccc ggggctcacc cccggaaggg cattggaaac tgacggactc
      121 gagtcccgga gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcgga ggcgaaggcg gccttctgga cggtgactga cgctgatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006684
VERSION     KHUT01006684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           46 t
ORIGIN      
        1 tacgagaggt ccaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcgggcctg
       61 taagtgttct gtgaaatccc atggctcaac catggaatcg cagggcatac tgcgggcctc
      121 gaggaagcta gaggccggtg gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaacgccaaa ggtgaagaca gccggctggg gctttcctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006685
VERSION     KHUT01006685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggcggattcg
       61 taagtcaggt gtgaaatctc ggggcttaac cccgaaagtg cgcctgaaac tgtgaatcta
      121 gaattctgga gagggaaggg gaatttctca tgtaggggta aaatccgtag atatgagaag
      181 gaacaccgga ggcgaaggcg ccttcctgga cagatattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006686
VERSION     KHUT01006686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttatt
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgagac tgaatagcta
      121 gggtacggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagtaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006687             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006687
VERSION     KHUT01006687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           48 t
ORIGIN      
        1 gacgaactgt gcgagcgtta ttcggagtca ctgggcttaa agcgcacgta ggcggcatcg
       61 taagttcggg gtgaaatcct gtcggctcaa ccgcggaatt gcctcgaaga ctgcgaagct
      121 ggagtgagat aggggtgtgt ggaacttccg gtggagcggt gaaatgtgtt gatatcggaa
      181 ggaacgccgg tggcgaaagc gacacactgg gtctctactg acgctgaggt gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006688
VERSION     KHUT01006688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggcttat
       61 caagtcagtg gtgaaatccg gcagcttaac tgtcgaagtg cctctgatac tgatgagctc
      121 gagggaggta ggggtgtgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006689
VERSION     KHUT01006689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccccg
       61 taagtcagcg gtgaaatccc ccggctcaac cggggaactg ccgttgatac tgcggggctt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag ataccgtcag
      181 gaaccccgat tgcgtaggca gcttactagg ctgttactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006690
VERSION     KHUT01006690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           94 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggcttcg
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggggagctt
      121 gagcgcagga gaggaaagca gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggtgaagacg gctttctgga ctgctgctga cgctgataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006691
VERSION     KHUT01006691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaaa
       61 taggtcagtt gtgaaaactg gaggctcaac ttccagacgt cgactgaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006692
VERSION     KHUT01006692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggttgttccc
       61 taagtcagat gtgaaagacc ggagctcaac ttcggaactg catttgatac tgggggactg
      121 gagggttcga ggggagtctg gaattcacgg tgtagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagt ggcgaaggcg ggactctgga gaacacctga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006693
VERSION     KHUT01006693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgg
       61 caagaaggat gtgaaatccc ggggcttaac ctcggaactg cattcttaac tgccgggcta
      121 gagtatgtca gaggggggta gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaataccgat ggcgaaggca gccccctggg ataatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006694
VERSION     KHUT01006694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           79 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcccga
       61 taagtcagcg gtgaaatctc ccggctcaac cgggaaatgg ccattgatac tgtcgggctt
      121 gaattatcag gaagcaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttgctact ggtggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006695
VERSION     KHUT01006695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           41 c           71 g           61 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcgggttta
       61 taagtcagtg gtgaaatctc tttgcttaac aaagaaactg ccattgatac tgtagaccta
      121 gagtatagat gatgttggcg gaatatgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccgat tgcgaaggca gctgacaaaa ctataactga cgctgatgca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01006696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006696
VERSION     KHUT01006696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcgggtgct
       61 cgagtcaggg gtgaaatgcc aaggctcaac cttggaactg cctttgatac tgggcgcctg
      121 gagatcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006697
VERSION     KHUT01006697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           98 g           40 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccgcg
       61 caagtcggat gtgaaaaccc ggggctcaac cccgggcctg cattcgaaac tgcgtggctt
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006698
VERSION     KHUT01006698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggtggctttt
       61 caagtccggt gtgaaatccc acggctcaac cgtggaactg cattggaaac tggatggctc
      121 gagggttgga gggggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggc caatcactga cactgaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006699
VERSION     KHUT01006699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           91 g           50 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtttgg
       61 caagtcgggc atgaaatctc tcggctcaac cgggagaggt tgtccgatac tgctagactt
      121 gagggcagta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaagcg gccttctggg ctgctcctga cgctaaagcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006700
VERSION     KHUT01006700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 gacgaggtgc acaaacgtta ttcggattta ctgggcttaa agcgtgcgta ggcggcctgg
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcccaaaac tgctgggctg
      121 gagtgagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcactggg tctctactga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006701
VERSION     KHUT01006701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggttcgt
       61 taagtcaggc gtgaaatgcc cgggctcaac ctgggaactg cgcttgatac tggcggacta
      121 gagtccggga gaggatggtg gaactcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacccgt ggcgaaggcg gccatctgga ccggtactga cgctaaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006702
VERSION     KHUT01006702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggaatta ttgggtttaa agggtgcgta ggcggaccta
       61 taagtcagtg gtgaaagccc caagctcaac ttgggaactg ccattgatac tgttggtctt
      121 gagtacagtt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttgctaag ctgtaactga cgctgatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006703
VERSION     KHUT01006703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           76 g           57 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggattca ctgggtttaa agggtgtgca ggtggggtaa
       61 taagtcagtg gtgaaagccc ccgactcaat cggggaactg ccatagatac tgttattctt
      121 gagtgtagtt gaggcggatg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat cgcgaaggca gtccgccaag ctattactga cactgaaaca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01006704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006704
VERSION     KHUT01006704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtttgg
       61 taagtgtgtg gtgaaagccc ggggctcaac cccgggtctg ccatgcaaac tgctaaactt
      121 gagcatacta gaggcaggta gaatttcggg tgtagcggtg gaatgcgtag agatccgaaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtagtgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006705
VERSION     KHUT01006705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccttg
       61 taagtctggt gtgaaagccc ggggcttacc cccggaagtg cactggaaac tgcaaggctt
      121 gagtaccgga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006706
VERSION     KHUT01006706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgccg
       61 taagtctggt gtgaaatccc ggagctcaac tccggaatgg cactggatac tgcggtgctc
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006707
VERSION     KHUT01006707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           82 g           55 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtttgg
       61 taagtcaaat gtgaaatcct acagcttaac tgtagaaatg cattcgaaac tgccaagcta
      121 gaggactgga gaggaaatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gatttctgga cagtttctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006708
VERSION     KHUT01006708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           80 g           57 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattga
       61 taagtcagtg gtgaaagccc gtcgcttaac ggcggaactg ccattgatac tgtcagtctt
      121 gagtatggtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006709
VERSION     KHUT01006709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcggcg
       61 taagtcgaat gtgaaagccc ttggcttaac caaggaagtg cattcgatac tgtgtcgctt
      121 gagtaccgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006710
VERSION     KHUT01006710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtgccg
       61 taagttggat gtgaaatccc ggagctcaac tccggaactg cattcaatac tgcggtgcta
      121 gagtactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006711
VERSION     KHUT01006711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           92 g           57 t
ORIGIN      
        1 tacgggtgag gcaagcgttg ttcggaatca ttgggcttaa agcgcgtgta ggcggcggcg
       61 taagtacttt gtgaaatccc ttctctcaat ggaggaattg cttggtatac tgcgttgctt
      121 gaggcaggta ggggtgcttg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccagt ggcgaaagcg gagcactggg cctgtcctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006712
VERSION     KHUT01006712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           85 g           54 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatca ttgggcgtaa agcgagtgcg ggtggctctt
       61 taagtcagga gtgaaagccc agggctcaac cctggaagtg cttttgatac tggagagctt
      121 gaatgtggca gagggtgcta gaatacctaa tgtagtggtg aaatacgtag atattaggtg
      181 gaacaccggt ggcgaaggcg ggcacctggg ccaacattga cacttagact cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006713
VERSION     KHUT01006713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgat
       61 ttagtcgcgt gtgaaagccc ccggctcaac tggggagggt cacgcgatac tgatcgactc
      121 gaaggcagga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01006714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006714
VERSION     KHUT01006714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgcg
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaactg catcttatac tgcgcagctg
      121 gagtgaagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga cttacactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006715
VERSION     KHUT01006715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccga
       61 atagtctggt gtgaaaactt gaggctcaac ctcaagctgt cactggatac tgtcgggctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006716
VERSION     KHUT01006716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcgcag
       61 caagtggggt gtgaaatccc tcggcttaac cgaggaattg cgctccaaac tgctgtgctt
      121 gaggaagcta gaggtgagca gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaataccgga ggcgaaggcg gctcactggg gcttatctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006717
VERSION     KHUT01006717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaagtg cctttgatac tgggaatctc
      121 gagggaggta ggggcgtgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006718
VERSION     KHUT01006718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           86 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatata
       61 taagtcagat gtgaaatacc cgggctcaac ttgggtgctg catttgaaac tgtatatcta
      121 gagtgcggga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ccgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006719
VERSION     KHUT01006719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           81 g           55 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcgggctgg
       61 taagtcagta gtgaaatctc ggcgctcaac gccgaaacgt cttttgatac taccagtctt
      121 gaataagcga gagatccgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccaga ggcgaaggca gcgggttggc gctttattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01006720             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006720
VERSION     KHUT01006720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttggt
       61 cgtgtcctgt gtgaaagccc cgggcttaac ctgggaatgg catgggagta ctggccggct
      121 ggagtgtggc agaggggggt ggaattccgc gtgtagcagt gaaatgcgta gagatgcgga
      181 ggaacaccga tggcgaaggc agccccctgg gctaacactg acgctcaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01006721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006721
VERSION     KHUT01006721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggcctcg
       61 cgagtcgggt gtgaaagccc ggggctcaac cccggacgtg cattcgatac tacgaggctc
      121 gagttctgaa gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagacactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006722
VERSION     KHUT01006722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           87 g           46 t
ORIGIN      
        1 tacagagact gcgagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccaaa
       61 tgtgtgaggc gtgaaagccc agggcttaac cctggaattg cacctcaaac tatttggcta
      121 gagcattgga gagggaagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gcttcctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01006723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006723
VERSION     KHUT01006723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c          102 g           38 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggctttt
       61 caagttcgtc gtgaaagacc ccggctaaac cgggggaggt cgagggagac tgagaagctg
      121 gagggcggca gagggacagg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg ctgtcctggg ccgcaactga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006724
VERSION     KHUT01006724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           94 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttgtg
       61 catgtcagat gtgaaagccc cgggcttaac ctgggaattg catttgaaac tgcgcggcta
      121 gaggccggaa gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctggt ccggttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006725
VERSION     KHUT01006725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           48 c           97 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtcgaaa gtgaaaacca tgggcttaac ccgtggcttg ctttcggtac gggcatgctt
      121 gagtgtggta ggggagtctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006726
VERSION     KHUT01006726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 tcagtctgct gtgaaagtcc agggctcaac cctgggatgc cagtggatac tgccaagcta
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006727
VERSION     KHUT01006727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          108 g           35 t
ORIGIN      
        1 cacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggttggt
       61 cgcatcggcg gtgaaagccc cccgcttaac gggggagggt ccgtcgagat gggccaactg
      121 gaggcaggga gagggcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctgga cctgacctga cgctgaggcg cgaaggcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006728
VERSION     KHUT01006728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           86 g           50 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggtgtaa agggcgcgta ggcggcagga
       61 aaagtcggat gtgaaattcc ggggctcaac cccggagctg catttgaaac tatccagcta
      121 gaggttaata agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gttttctgtg ttaaacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006729
VERSION     KHUT01006729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggctgcgggg
       61 aaagtcagat gtgaaatccg gaggctcaac cttcgaatag catccgatac taccttgcta
      121 gagagctgta ggggggtttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgt ggcgaaggcg aaaccctggg cagtatctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006730
VERSION     KHUT01006730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcgcag
       61 taagtcccat gtgaaagccc acggcttaac cgtggaagtg catgggaaac tactgtgctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagttactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006731
VERSION     KHUT01006731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cccttgatac tggcaatcta
      121 gagactggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccagtactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006732
VERSION     KHUT01006732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgt
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctttgatac tgacaggctc
      121 gagggaagta ggggcatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cttctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006733
VERSION     KHUT01006733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           86 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttat
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaattg catatgatac tggtgaacta
      121 gagtgcagaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctggt ctgttactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006734
VERSION     KHUT01006734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcccag
       61 taagtccgct ctgaaagtca caggcttaac ctgtgaaagc gggtggatac tgctgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006735
VERSION     KHUT01006735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctca
       61 aaagtcaggg gtgaaatccc acggcttaac cgtggaagtg cctttgatac tgtgagtctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006736
VERSION     KHUT01006736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgatg
       61 taagttggat gtgaaatccc agggcttaac cctggaactg cattcaatac tgcgttgcta
      121 gagtactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006737
VERSION     KHUT01006737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           94 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatga ctgggcgtaa agggtgcgta ggtggtctgt
       61 taagttgttg gtgaaatctc cgggctcaac ctggggttga cgaataaaac tgatggactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt tgcgaaggcg gctacctggc ctggtactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006738
VERSION     KHUT01006738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          100 g           40 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtaa agcgcacgta ggtggtacgg
       61 cgcgtggggt gtgaaatcgg ccggcttaac cggtcgccgc catcccaaac ggccgcactc
      121 gagccacata gaggtcgctg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggggaagcca ggcgactggg tgtgtgctga cactgaggtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01006739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006739
VERSION     KHUT01006739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgacc
       61 caagtccgtg gtgaaagctc gtcgcttaac gacgagtcgg ccacggagac tgggtggctt
      121 gagcactgta ggggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gttagctggg cagttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006740
VERSION     KHUT01006740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccatc
       61 aaagtcgatt gtgaaaactc ggggctcaac cccgagactg cgttcgatac tggatggcta
      121 gaggctggta ggggggtgtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gcaccctggg ccagtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006741
VERSION     KHUT01006741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctc
       61 caagtccggg gtgaaatcct cccgcccaac gggagaagag cctcggagac tgggggcctc
      121 gagggaggta ggggcgtgcg gaactatggg tggagcggtg aaatgcattg atatccatag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006742
VERSION     KHUT01006742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttcgt
       61 taagttcgat gttaaagacc ggggcctaac cccgggactg cattgaatac tggcggactg
      121 gagtgcagta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgtaagcg acttgctggg ctgtaactga cgctgaggaa cgaaagccag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01006743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006743
VERSION     KHUT01006743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           83 g           56 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggtttgt
       61 taagtttgtt gttaaagact ttggcttaac tgaagaaaag caacaaatac tggcgaacta
      121 gagggtgaaa gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggct gcttcttggt tcactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006744
VERSION     KHUT01006744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtgcctt gtgaaatccc ccggcttaac cggggaatgg ctgggcagac tgggagtctt
      121 gagtggccta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg ggtcaactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006745
VERSION     KHUT01006745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agagttcgta ggcggctcca
       61 taagtcgggt gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcggagctt
      121 gaatatggta gaggagagta gaattgctgg tgtagtggtg aaatacgtag atatcagcag
      181 gaataccggt ggcgaaggcg gctctctgga ccaatattga cgctgaggaa cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01006746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006746
VERSION     KHUT01006746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttctc
       61 caagtctgct gtgaaagccc cgggcttaac ctgggaatgg cagtggaaac tataggactg
      121 gaatctggca gaggggggtg gaatgccacg tgtagcagtg aaatgcgtag agatgtggcg
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006747
VERSION     KHUT01006747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          100 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcgggcttc
       61 taagtgagag gtgaaaggtt cgggctcaac ccggagagtg cctttcatac tgggagcctt
      121 gagtgcggga gaggcaagtg gaatggtcgg tgtagcggtg aaatgcgtag atatcgatcg
      181 gaacacccgt ggcgaaggcg gcttgctggc tcgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006748
VERSION     KHUT01006748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcaacg
       61 taagtcagag gtgaaagctt cccgcttaac gggagaactg cctttgatac tgcgtagctt
      121 gaatatgtgc gaggcaggcg gaatgtggca tgtagcggtg aaatgcttag agatgccata
      181 gaacaccgat tgcgaaggca gcctgccagc actttattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006749
VERSION     KHUT01006749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg tcaagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggctcga
       61 taagtttcag gtgaaatctc ccggctcaac tgggaggggt cctgaaaaac tatcgggctt
      121 gagtcatgga gagggatgtg gaattccagg tgtagtggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcatcctggc catgtactga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006750
VERSION     KHUT01006750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgaag
       61 caagtcagtg gtgaaagctc gacgcttaac gtcgagtcgg ccactgatac tgcatcgctt
      121 gagcactgta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006751
VERSION     KHUT01006751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggctgt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccaatgatac taatagtctt
      121 gaatgttgtt gaggtaggcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccgat tgcgaaggca gcttactaaa caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006752
VERSION     KHUT01006752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          102 g           39 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacgc
       61 caagtacgga gtgaaatctc cctgcctaac ggggagggtg ctctgtaaac tggcgagcta
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006753
VERSION     KHUT01006753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           85 g           56 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggtatcg
       61 taagtcagat gtgaaagttt gaggctcaac ctcaaaattg catttgaaac tgcaatactt
      121 gagtactgga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga cagttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006754
VERSION     KHUT01006754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          106 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agggcgcgta ggcggggtgc
       61 cacgtccgct gtgaaagcct gacgcttaac gtcagcgggt cggtggatac gggcactctt
      121 gaaggcgtta ggggaagatg gaattacccg tggagcggtg aaatgcgtag agatgggtag
      181 gaacaccggt ggcgaaggcg gtcttctggg acgtccttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006755
VERSION     KHUT01006755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacgagaggc gcaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac taccgtgctt
      121 gaggaagcta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006756
VERSION     KHUT01006756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggaccta
       61 caagcctttg gtgaaagcct ggagctcaac tccagaactg ccgaagggac tgtgggtctg
      121 gaggccggca gaggagagtg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacacccat ggcgaaggca gctctctggg ccggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006757
VERSION     KHUT01006757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           86 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttatt
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg catttgaaac tagatggcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccattactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006758
VERSION     KHUT01006758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc tgagcttaac tcggaaactg ccattgatac tatcagtctt
      121 gaatattgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctacg catatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006759
VERSION     KHUT01006759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          103 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggcctgt
       61 taagtcaggg gtgaaagacc ggggctcaac cccgggggtg cctctgaaac tggcgggctt
      121 gagtgctcta gagggcagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg gagtcactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006760             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006760
VERSION     KHUT01006760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c          102 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtggg
       61 aacgtcggcc actgaaagcc cccggctcaa ccggggaagg ggtgccgaga cggcccgcct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtcatctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006761
VERSION     KHUT01006761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 gacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagtgc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tggttctctt
      121 gggccactta ggggctactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca agtagctggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006762             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006762
VERSION     KHUT01006762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           80 g           60 t
ORIGIN      
        1 tacagaggtt acaagcgttg tccggattta ttgggcgtaa agcgtttcgt aggcggttag
       61 gaaagttact tttcaaatac cacggctcaa ccgggggaag ggaggtaata ctatctgact
      121 tgattattgg tggggcttct ggaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaactccaa gggcgaaggc aggaagctaa ccaattaatg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01006763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006763
VERSION     KHUT01006763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           87 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcggag
       61 taagtcgggt gtgaaatttg agggctcaac ccttaacttg cacctgatac tgcttcgctc
      121 gaggcctgaa gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag atttcgagaa
      181 gaacgctcgc agcgaaagcg aagctctggt caggatctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006764
VERSION     KHUT01006764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctgcg
       61 taagtcggat gtgaaagccc caggctcaac ctgggaagtg cactcgatac tgcgcagctt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006765
VERSION     KHUT01006765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           89 g           46 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggcatga
       61 caagttcgat gtgaaagccc cgggcttaac ccgggaatgg cattgaatac tgccgtgctg
      121 gaggattgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caatacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01006766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006766
VERSION     KHUT01006766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          106 g           36 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgtgcgca ggcggcccgc
       61 gaggtgtcac gtgaaagccc cgggctcaac ccgggagggt cgtggcaaac cggcgggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtactga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006767
VERSION     KHUT01006767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtcttg
       61 taagtctact gtgaaatccc cgagcttaac ttgggaagtg cggtggatac tgcaagacta
      121 gagtgctgga ggggttcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga cagcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006768
VERSION     KHUT01006768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           97 g           52 t
ORIGIN      
        1 tacgtgaggg gcgaacgttg ttcggtgtca ctgggcttaa agggcgcgta ggtggttgcg
       61 taagtaacgt gtgaaatccc tcggcttacc cggggaattg catgttatac tgcgtggctt
      121 gaggatcaca ggggaaagcg gaactccagg tgtagcggtg aaatgcgcag atatctgggg
      181 gaaggccggc ggcgaaggcg gctttctggg tgattcctga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006769
VERSION     KHUT01006769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          105 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggattga ctgggcgtaa agggcgtgta ggcggttcgt
       61 taagtcgggt gtgaaagccc ggggctcagc tccggaagtg cgcccgaaac tggcgagctg
      121 gagcgcggta gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccctctgga ccgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006770
VERSION     KHUT01006770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctgag
       61 caagtcgaac gtgaaatccc tgggcttaac ccaggaagtg catttgatac tgctcaactt
      121 gagtcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006771
VERSION     KHUT01006771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           91 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agagaacgta ggcggatttg
       61 tatgtcgatt gtgaaagccc agggctcaac cttggaattg cagtcgaaac tgcggatctt
      121 gaatacttga gagggtgggg gaattcctgg tggagaagtg aaattcgtag agatcaggag
      181 gaacatcgga ggcgaaggcg cctgcctggc aatgtattga cgctgaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006772
VERSION     KHUT01006772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           82 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgca ggcggtctgt
       61 taagtgagat gttaaatcgt gtggctcaac catatcgatg catctcatac tggcagacta
      121 gaggaaggca gaggcaagtg gaattggtag tgtagcagtg aaatgcgtag atattaccag
      181 gaacaccaat ggcgaaggca gcttgctggg ccttatctga cgctcaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006773
VERSION     KHUT01006773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgg
       61 taagacaggc gtgaaatccc cgagctcaac ttgggaatgg cgcttgtgac tgccaggcta
      121 gagtatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg acgtcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006774
VERSION     KHUT01006774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ctgggcgtaa agggttcgta ggcgggaatg
       61 taagttagat gtgaaaactt cgggctcaac ctggaacgtg catctaaaac tacatttctt
      121 gagtgttgga gagggcagtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaggcg actgcctggc cgaacactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006775
VERSION     KHUT01006775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agcgtacgca ggcggtccaa
       61 tcagtctgta gggacagggc acagcttaac tgtgtcaggc ttatggaaac tgttggactt
      121 gagggcttca gagggatggg gaattccggg tggagtggtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgtaggca ccatcctggg gagtcactga cgctcatgta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006776
VERSION     KHUT01006776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          101 g           41 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcagattga ctgggcgtaa agggcgtgta ggcggtcggt
       61 caagtccgat gtgaaagccc ggggctcagc cccggaaggg catcggaaac tagccggctg
      121 gagcgcgata gaggagggta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggc ggcgaaggcg gccctctgga tcgttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006777
VERSION     KHUT01006777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtcggat gtgaaatctc ggagcttaac tccgaaactg cattggaaac taccttgctc
      121 gagggttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006778
VERSION     KHUT01006778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           83 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgtgta ggctgcgtgt
       61 taagtcagat gtgaaatccc ggggctcaac ctcggaactg catccgatac tggcatgcta
      121 gagaattgga gaggagtcta gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaataccaag tgcgaaggca ggactctgga cattttctga cgctgagaca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006779
VERSION     KHUT01006779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggctcat
       61 agagtcgggc gtgaaattcc tgggcttaac ctgggggctg cgttcgatac ctatggactg
      121 gagtgaggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgtaggcg gcgacctggt cctttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006780
VERSION     KHUT01006780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgca ggcggctgat
       61 taagtcggat gtgaaaggcc acggctcaac cgtggagttg catccgaaac tggttggctt
      121 gagtgcagga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006781
VERSION     KHUT01006781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           89 g           54 t
ORIGIN      
        1 tacgtaggtt gcgagcgtta ttcggattta ctgggcgtaa agcgcaccga ggtggcttct
       61 taagttggat gtgaaatctc ccggcttaac tgggaggcgc cattcaaaac tggggagctt
      121 gagaccagca ggggaaagtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg actttctggg ctgcgtctga cactctagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006782
VERSION     KHUT01006782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgca ggcgggccga
       61 taagtcagtg gtgaaatctc atagcttaac tatgaaactg ccattgatac tgtcggtctt
      121 gagtaaatta gaggtagctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggttaccatg atttaactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006783
VERSION     KHUT01006783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtttggt gtgaaagctc cgagctcaac tcggaaatgg catcgaatac tatctcgcta
      121 gagggttgta ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006784             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006784
VERSION     KHUT01006784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           73 g           66 t
ORIGIN      
        1 aacggggggg actagtgtta ttcaacctga ctgggcgtaa agggcatgta ggcggcctat
       61 tatgttttta ataaaattct tgggctcaac ctgagggctg ttatttaaac tgataagctt
      121 gagtttatac gagcaagacg gtacacatgg tgtagaggtg atattcgaag aaattatgtg
      181 gaccaccaac gggcgaaggc ggttttgcag ttattaactg acgctaaagt gcaaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006785
VERSION     KHUT01006785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           97 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtctgct gtgaaagccc ggggcttaac tccgggtctg cagtgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccattactga cgctgagaag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01006786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006786
VERSION     KHUT01006786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 tacgaactgc gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgaa
       61 caggtccggt gtgaaatccc acagctcaac tgtggaatgg cattggaaac ctttcggctt
      121 gagtgggcta gaggtgcacg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg gtgcactgga gcttgtctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006787
VERSION     KHUT01006787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggttggg
       61 ttagtcagac gtgaaatctc accgcttaac ggtgaaactg cgtttgatac tgcccgacta
      121 gaggatcgga gggggaagcg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gcttcctgga agattcctga ctctgaaaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006788
VERSION     KHUT01006788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacggggggt ccaagcgttg tccggaatca ctgggcgtaa agcgaatgta ggcggctatt
       61 caagtcactt gtgaaagccc ggggcctaac cccggaaggg caagtgatac tgtttagctt
      121 gagtatcaca gaggacactg gaatacatgg tgtagtggtg aaatacgtag atatcatgtg
      181 gaacaccggc ggcgaaggcg ggtgtctggg tgaatactga cgctgagatt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006789
VERSION     KHUT01006789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           86 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttata
       61 taagacagtt gtgaaatccc cgggctcaac ctgggaattg catctgtgac tgtatagcta
      121 gagtacggta gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006790
VERSION     KHUT01006790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 ccagtctgtg gtgaaatcct ccagcctaac tggagaatgg ccatggatac tgcgggtctc
      121 gagggaggta ggggcgtgcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006791
VERSION     KHUT01006791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           88 g           50 t
ORIGIN      
        1 cacgaaccgt ccaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggcgctt
       61 taagtcgggt gtgaaatcct tcggctcaac cgaagaattg cgcccgatac tggagtgcta
      121 gagggagaca ggggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006792
VERSION     KHUT01006792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggtggttgat
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaagtg ccttggaaac tgatcagctt
      121 gagggatgta ggggcagttg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg aactgctgga catcttctga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006793
VERSION     KHUT01006793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           93 g           54 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtcatt
       61 taagttactt gtgaaattcc tgggctcaac ctggggtgga cgattaatac tggatgactt
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctgcctggc ctaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006794
VERSION     KHUT01006794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 tacagagact ccaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggtcaag
       61 tgtgtcagat gtgaaagctc cgggcttaac ccggaaattg cgtctgaaac tatttggcta
      121 gagtattgga gagggaagcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccaaa ggcgaaggca gcttcctgga caattactga cgctcatgca cgaaagcgtg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01006795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006795
VERSION     KHUT01006795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           83 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggcggttaga
       61 taagttagat gtgaaatccc cgggcttaac ctgggcactg cgtttaagac tgtttaacta
      121 gagtgctgta gagggtagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actacctgga cagacactga cgctgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006796
VERSION     KHUT01006796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtatgca ggcggatctc
       61 taagtcgggg gtgaaatcct gaggctcaac ctcagaactg ccttcgatac tggagatctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006797
VERSION     KHUT01006797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gtcggctttt
       61 caagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgatac tggatcgcta
      121 gagttcggca gagggaagcg gaatttcggg tgtagcggtg aaatgcgtag atatccgaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccagaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006798
VERSION     KHUT01006798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          103 g           45 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcttg
       61 taagtctgtg gtgaaagccc ggggctcaac cccgtgtctg ccatggatac tgtgagactt
      121 gagtgctgta gaggcgggta gaatgccggg tgtagcggtg gaatgcgtag agatccggca
      181 gaagaccggt ggcgaaggcg gcccgctggg cagtaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006799
VERSION     KHUT01006799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtacga
       61 caagtctgga gtgaaagccc ggggctcaac cccggaatgt ctttggaaac tgtcgaactt
      121 gagtgcggaa gaggcatctg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggatgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006800
VERSION     KHUT01006800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgtta
       61 caagtgagat gtgaaatccc tgggcttaac ctaggaatgg cattttagac tgtgacgcta
      121 gaggtcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgaatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006801
VERSION     KHUT01006801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagcctg cattcgatac tgacgggcta
      121 gagtactgga ggggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006802
VERSION     KHUT01006802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcggatcgt
       61 taagtcgggt gtgaaatccc ggggctcaac cccggaactg cattcgaaac tggcggtctt
      121 gagtacggga gagggaggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcctcctggc ccgatactga cgctcatgta cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006803
VERSION     KHUT01006803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           91 g           45 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatta ctgggcttaa agcgcacgta ggcggcattg
       61 caagtgtccg gtgaaatccc acggctcacc cgtggaactg cctggcagac tgcaatgctc
      121 gagtgtggta ggggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccacaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006804
VERSION     KHUT01006804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgagga ggcggcacga
       61 caagtctgat gtgaaagccc tgggcttaac ccaggaactg catcggaaac tgtcgagcta
      121 gagttcggga gagggtcgtg gaattcttgg tgtagaggtg aaattcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gcgacctgga ccgatactga cgctaataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006805
VERSION     KHUT01006805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           88 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcgggcata
       61 taagtcagtg gtgaaaggcc agggctcaac tctggatgtg ccattgatac tgtaagtctt
      121 gagagcagat gaggtagctg gaatgtgtag tgtagcggtg aaatgcatag agattacaca
      181 gaacaccgat tgcgaaggca ggttactagt ctgtatctga cgctgaggca cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006806
VERSION     KHUT01006806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           78 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcgtc
       61 taagtcagtg gtgaaattct gcagctcaac tgtaggactg ccattgatac tggtcgcctt
      121 gaatcgttgt gaagtggcta gaatatgtag tgtagcggtg aaatgcttag atattacata
      181 gaataccgat tgcgaaggca ggtcactaac aacgcattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006807
VERSION     KHUT01006807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 tacagaggcc ccgagcgtta gtcggaatta ctgggcttaa agggtgcgta ggcggacttc
       61 taagtgtcgc gtgaaagccc acggcttaac cgtggaaccg cgcggcaaac tgggagtctt
      121 gaggaacgta taggcgggtg gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaacgccgaa ggtgaagaca gcccgctggg cgtttcctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006808
VERSION     KHUT01006808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcggag
       61 caagtcgggt gtgaaatttg ggagctcaac tcccaactag cacctgatac tgctttgcta
      121 gagatctgaa ggggagcttg gaattctcgg tgtagcagtg aaatgcgtag atttcgagaa
      181 gaacgctcgc agcgaaagcg aagctctggt cagattctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006809
VERSION     KHUT01006809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           56 c          108 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggctcga
       61 caagtcggcc gtgaaatctc caggctcaac ttggaggggt cgggcgagac tgtcgggctc
      121 gaggtgggca gagggcagtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctgcctggg cctggcctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01006810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006810
VERSION     KHUT01006810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtttgta ggcggctcta
       61 taagtcgggt gtgaaagccc acggctcaac cgtggaagtg cattcgaaac tgcagagctt
      121 gaatattgaa gaggacagta gaattgctgg tgtagtggtg aaatacgtag atatcagcag
      181 gaataccggt ggcgaaggcg actgtctggt caaatattga cgctgagaaa cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01006811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006811
VERSION     KHUT01006811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggattta ctgggcgtaa agcgcgtgta ggcgggttcg
       61 caagtcggac gtgaaagccc tgggctcaac ccaggaactg cgttcgaaac tgcgaatcta
      121 gagtagtgga gaggaaagta gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006812
VERSION     KHUT01006812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 cacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgatt
       61 caagtcagat gtgaaatgcc tgagctcaac tgaggcattg cgtttgaaac tgaattgcta
      121 gagtatccta ggggtgtgcg gaactgccag tggaacggtg aaatgtgttg atattggcag
      181 gaacaccgga ggcgaaagcg gcacactggg ggataactga cgctgaggca cgaaagccaa
      241 gggagcgaac ggg
//
LOCUS       KHUT01006813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006813
VERSION     KHUT01006813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcggttcgt
       61 taagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cattcgaaac tggcgagcta
      121 gaggacagga gaggggagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatcggt ggcgaaagcg gctccctggt ctgttactga cgctcatgta cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006814
VERSION     KHUT01006814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgc
       61 taagttgcgg gtgaaatccc ggggcttaac tccggggctg ccagtaagac tggcaggcta
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg atctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006815
VERSION     KHUT01006815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           82 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggctggtttg
       61 taagtcagat gtgaaatccc ggggctcaac ctcggaacag catttgaaac tacataccta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01006816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006816
VERSION     KHUT01006816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           99 g           46 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggttcgg
       61 taagttggat gtgaaagccc ggggcttaac cccggatgtg cgttcaagac tgccgaactt
      121 gagaccggaa ggggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006817
VERSION     KHUT01006817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           75 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtctaa
       61 taagtcagtg gtgaaagccc tacgctcaac gtaggaacgg ccattgatac tgttagactt
      121 gaattacttg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactaca agtggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006818
VERSION     KHUT01006818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtatgta ggcggttagt
       61 taagtcaaat gttaaagacc gaggctcaac ctcgggaatg cctttgatac tggctaacta
      121 gagggtgtta gaggaaggcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacatcggt ggcgaaagcg gccttctggg acatacctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006819
VERSION     KHUT01006819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctag
       61 caagtcagat gtgaaatccc ggggcttaac tccggacgtg catttgaaac tgctaggctc
      121 gagtgtagta gaggttggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006820
VERSION     KHUT01006820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           85 g           55 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggttcgg
       61 taagttgggc gtgaaatctc ctggcttaac taggagaggt cgttcaatac taccggactt
      121 gagagtgata gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg atcttctgga tcatttctga cgctcagacg cgaaagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01006821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006821
VERSION     KHUT01006821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttctg
       61 taagttggag gtgaaatccc tgggcttaac ctgggcacgg cctccaaaac tgtaggactc
      121 gagtcgagta gagggaggta gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaataccagt ggcgaaggcg gcctcctgga ctcagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006822
VERSION     KHUT01006822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggttttc
       61 caagtcgggc gtgaaagccc cgggctcaac ccgggaaatg cgttcgaaac ttgaagactg
      121 gaggccggaa gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctggt ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006823
VERSION     KHUT01006823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggcttg
       61 taagtgtcct gtgaaatccc ccggctcaac cggggaactg cggggcaaac tgcaagtctt
      121 gaggaagcta gaggccggta gaacgattgg tggagcggtg gaatgcgtag atatcaatcg
      181 gaatgccgaa agtgaagaca gccggctggg gcttttctga cgctgaagca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006824
VERSION     KHUT01006824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctctg
       61 caagtcaggt gtgaaagccc tgggctcaac ctaggaagtg cacttgaaac tgcggagctt
      121 gaatgtcgga gagggcagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctgcctgga cgacgattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006825
VERSION     KHUT01006825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           88 g           53 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ctgggcgtaa agggtgcgta gatggtatgt
       61 taagttcgtt gttaaaggct ccggcctaac tggagtattg cgatgaaaac tggcgcacta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cattgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006826
VERSION     KHUT01006826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcagag gtgaaatctt tcggcttaac cgaaaaagtg cctttgatac tggcgagcta
      121 gagggaggta ggggtatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006827
VERSION     KHUT01006827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           92 g           43 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggttcct
       61 taagtcgaat gtgaaatcct gcggcccaac cgcggaactg catccgatac tggggaactt
      121 gaggacggta gaggtcggtg gaattccagg tgtaccggtg aaatgggcag atatctggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagctag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01006828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006828
VERSION     KHUT01006828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgct
       61 caagtcggat gtgaaaatcc ggggctcaac cccggacctg cattcgatac tgggcggcta
      121 gaggcaggta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006829
VERSION     KHUT01006829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcggac gtgaaaactc ggggctcaac tccgagcctg cgttcgatac gggcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgacactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006830
VERSION     KHUT01006830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           87 g           58 t
ORIGIN      
        1 tacgtaggtg gctagcgtta tccggattta ctgggcgtaa agagggtgca ggcggctctt
       61 caagtcggtt ataaaatctc ttggcttaac taagagggac tgaccgatac tgttgggctg
      121 gagagtagca gagggagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaagcg gtctcctagg ctatttctga cgctgaggcc ctaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006831
VERSION     KHUT01006831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgagta ggcggtctct
       61 taagtctgat gtgaaagccc ggggcttaac cccggaagtg cattggatac tgggagacta
      121 gagggtgtaa gaggagagtg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatcggt ggcgaaggcg gctctctggt acacttctga cgctcagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01006832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006832
VERSION     KHUT01006832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggccggg
       61 taagtctggt gtgaaatccc tgagctcaac tcaggaacgg cactggatac tatccggctc
      121 gaggtgtgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacaccagt ggcgaaagcg agtccctgga caccacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006833
VERSION     KHUT01006833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggatttt
       61 taagtcgggg gtgaaatccc agagctcaac tctggaactg ccttcgatac tgggaatctg
      121 gacccaagta gaggcgagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg actcgctgga cttgtggtga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006834             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006834
VERSION     KHUT01006834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           93 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cacgtcgtct gtgaaattcc ggggctcaac ctcgggcgtg caggcgatac gggcaatact
      121 tgagtgctgt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa tggcgaaggc aggtctctgg gcagtcactg acgctgagga gcgaaagcat
      241 gggtagcgaa cagg
//
LOCUS       KHUT01006835             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006835
VERSION     KHUT01006835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           51 t
ORIGIN      
        1 tacataggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaactt tgggctcaac ccaaagcctg catccgatac tgctgtgact
      121 agagttcggt aggggagcgg ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccgg tggcgaaggc gccgctctgg gccgatactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01006836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006836
VERSION     KHUT01006836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 gacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggttggg
       61 taagtcaggt gtgaaatctc agagcttaac tctgaaactg cgcttgatac tgctcagcta
      121 gaggatcgga gggggtaacg gaatttatgg tgtagcagtg aaatgcgtag atatcataag
      181 gaacaccggt ggcgaaggcg gttacctgga agattcctga ctctgaaaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006837             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006837
VERSION     KHUT01006837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           44 t
ORIGIN      
        1 tacggggggg ggcaagcgtt gttcggaatt actgggcgta aagggctcgt aggcggccaa
       61 ctaagtcaga cgtgaaatcc ccaggctcaa cctgggaact gcgtctgata ctggatggct
      121 tgaatccggg agagggatgc agaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaataccgg tggcgaaggc ggcatcctgg accggcattg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006838             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006838
VERSION     KHUT01006838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           97 g           52 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggctgga
       61 taagtctggc gtgaaatctt caccgcttaa cgggaagggg tcgctggaaa ctgttcagct
      121 tgagtatggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacaccgg tggcgaaggc ggcttgctgg gccatcactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006839             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006839
VERSION     KHUT01006839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           62 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggggcgg
       61 cacgtcggtc gctgaaatcc cccggcttaa ccggggaacg ggcgccgata cgaccgtcct
      121 ggaggaacgt agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagctcctgg acgttttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006840             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006840
VERSION     KHUT01006840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggttgcc
       61 taagtctggg gtgaaaggtt cagggcttaa cccgaacagt gccttggata ctgggcgact
      121 tgagtgccga agaggaaagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctttctgg tcggcaactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006841
VERSION     KHUT01006841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           43 c           94 g           55 t
ORIGIN      
        1 tacgtatggg gcgagcgttg ttcggattta ttgggcgtaa agggcgtgta ggcggctcat
       61 caagtctggt gtgaaaacgc aaggcttaac cttgcatagg cgctggaaac tggtgggctt
      121 gagttcttga ggggaagctg gaattccagg tgtaggggtg aaatctgtag atatctggaa
      181 gaacaccggt ggcgaaggcg agcttctatc agaggactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006842
VERSION     KHUT01006842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcatgta ggcggtcttg
       61 taagtcggac gtgaaagccc ggggctcaac cccggaagcg cgctcgatac tgcttgactt
      121 gggttctgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagataccga cgctgagatg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01006843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006843
VERSION     KHUT01006843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgcgg
       61 taagtcaggt gtgaaagccc tgggctcaac ctaggaagtg cacttgaaac taccgtgctt
      121 gagatctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagcatctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006844
VERSION     KHUT01006844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggttatg
       61 tgggtgtgat gtgaaagcct tgggcttaac ctgagaagtg catcgcaaac tgtataactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006845             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006845
VERSION     KHUT01006845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           42 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcaggt gtgaaacctc cgggctcaac ccggagacgc cacctgatac tgctgtggct
      121 agagtccagt aggggagcgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccag tggcgaaggc ggcgctctgg gctggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006846
VERSION     KHUT01006846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c          101 g           35 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtcgcg
       61 cgcgtggggt gtgaaagcgt gccgcttaac ggcgcgaggc cacctcatac ggcgcgacta
      121 gagccgtgca gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg cacgtgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006847
VERSION     KHUT01006847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c          102 g           35 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtggcg
       61 cgcgtggggt gtgaaaacgg gccgcttaac ggcccgaggc cacttcatac ggcgcgactg
      121 gagcctggca gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg ccagtgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006848
VERSION     KHUT01006848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           93 g           43 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ctcggaatca ctgggcataa agcgcgcgta ggtggcctga
       61 taagtcggcg gtgaaatccc atcgctcacc gatggaactg ccgccgatac tgtcgggctt
      121 gaggacggaa ggggagagtg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggg ccgttcctga cactgatgcg cgagagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006849
VERSION     KHUT01006849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggcgtaa agcgttcgta ggcggcgtgg
       61 taagtcagat ttgaaatatc ggggctcaac cccggacccg gatctgaaac taccgtgctt
      121 gagcagatca gagggcagtg gaattcccgg tgtagcggta atatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg gtcttgctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006850
VERSION     KHUT01006850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           90 g           47 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgccgatctt
      121 gtgccaccta ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtgtgcaga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006851
VERSION     KHUT01006851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacgtagagt gcaaacgttg ttcggaatta ctgggcataa agcgcttgta ggtggcgcgg
       61 taagtcggtt gtgaaagccc ctggctcaac cagggaattg ctgccgaaac taccgtgctt
      121 gagtgtgaga ggggagagtg gaacttccag tggagcggtg aaatgcgtag atattggaag
      181 gaacgccggt ggcgaaagcg actctctggc tcactactga cactgaggag cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006852
VERSION     KHUT01006852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgattcga
       61 tgagtcttat gtgaaagccc acagcttaac tgtggaagtg cacgagatac tgtcgaattt
      121 gagttcagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006853
VERSION     KHUT01006853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggtccgta ggcggttcgg
       61 taagtctctt gtgaaaaccc caggctcaac ttggggacgc cgagggatac tgccggactt
      121 gagattgtca gaggcgaacg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaactccagt ggcgaaagcg gttcgctggg acacatctga cgctgaggga cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01006854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006854
VERSION     KHUT01006854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 gacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcctgt
       61 taagtagggt gtgaaatccc ccggcccaac cggggaatgg cgctctaaac tagcaggctc
      121 gagtgagcta ggggtgcatg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg atgcactggg gcttttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006855
VERSION     KHUT01006855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          100 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggcttgt
       61 tgcgtcgact gtgaaagccc ggggcttaac tccgggtctg cagtcgatac gggcaggcta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaagag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006856
VERSION     KHUT01006856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           86 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ctgggcgtaa agagctcgta ggcggttaat
       61 taagtctaat gtgaaagccc tgggctcaat ccaggaagtg cattagatac tggttgactg
      121 gagtgcagga gaggaaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga agcgaaggcg gctttctgga ctgtcactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006857
VERSION     KHUT01006857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 tacagaggag gctagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggtggcctga
       61 caagtatctt gtgaaatccc cgggctcaac tcgggaaatg ctgggtatac tgtcgggctt
      121 gaggtaggta ggggtgcctg gaactctagg tggagcggtg aaatgcgtag agatctagag
      181 gaacgcccgt ggcgaaagcg gggcactggg cctgtcctga cactaagacg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006858
VERSION     KHUT01006858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           81 g           53 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggtcatg
       61 ttagtcagtt gttaaaacct ggggcttaac cccagatgtg cgactgaaac ggcataacta
      121 gaggatggca gagatgtatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtacattggg ccactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01006859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006859
VERSION     KHUT01006859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgagga ggcggctcgt
       61 taagtctgat gtgaaagccc tgggcttaac ccaggaattg catcggaaac tagcgggcta
      121 gagttcggga gagggtcgtg gaattcttgg tgtagaggtg aaattcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gcgacctgga ccgacactga cgctaataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006860
VERSION     KHUT01006860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           98 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggaccgc
       61 caagtcaggg gtgaaagcct ccggctcaac cggagaagag cctttgatac tggcggtcta
      121 gagggaggta ggggcctgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgggctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006861
VERSION     KHUT01006861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgatac tggcaggcta
      121 gagcgaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006862
VERSION     KHUT01006862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggctgt
       61 taagtcagcg gtgaaatccc ccggctcaac cgggggcgtg ccattgatac tggcggcctt
      121 gagtacagac gaggtgggcg gaatttacgg tgtagcggtg aaatgcatag ataccgtaaa
      181 gaaccccgat agcgtaggca gcccactagg ctggcactga cgctgaggca cgaaagcatg
      241 gggatcgaac agg
//
LOCUS       KHUT01006863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006863
VERSION     KHUT01006863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctctg
       61 caagtcgggg gtgaaatccc acagcttaac tgtggaagtg ccttcgatac tgcggagctc
      121 gagtgaggta ggggcagagg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ctctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006864
VERSION     KHUT01006864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c          103 g           35 t
ORIGIN      
        1 tacagagggc gcgagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacga
       61 caagtcggaa gtgaaatccc ggggctcaac tccggggctg cttcggaaac tgtcgacctg
      121 gggaccggta ggggtcggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccgactggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006865
VERSION     KHUT01006865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggtggccgac
       61 taagtcggac gtgaaatccc tcagcttaac tggggaactg cgtccgatac tggatggctt
      121 gagtgcagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctgcaactga cactgagaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006866
VERSION     KHUT01006866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           97 g           44 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcggat gtgaaatcct acggctcaac cgtggaactg cattcgatac tgtcgggcta
      121 gaggcaggta ggggagattg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006867
VERSION     KHUT01006867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          101 g           42 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agagcgtgta ggcggatggg
       61 caagtctggc gtgaaagctc ccggctaaac tgggagattg cgttggagac tgcgtatcta
      121 gaggcaggta gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acgtcctggg cctgacctga cgctgagacg cgacagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006868
VERSION     KHUT01006868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtgcgt
       61 taagtcggtg gtgaaatact tcggctcaac cggagaattg ccgctgaaac tggcgcgcta
      121 gagggaggta gaggtgcgtg gaacttccgg tggagtggtg aaatacgttg atatcggaag
      181 gaacgcctgt ggcgaaagcg atgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006869
VERSION     KHUT01006869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          104 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 taagtagggg gtgaaatccc gtggctcaac tgcgggacgg cctcctagac tgcctggctc
      121 gagcacggta gaggcaggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006870
VERSION     KHUT01006870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           98 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcacgc
       61 taagtatttt gtgaaatctc cctgctcaac ggggagagcg cgaagtaaac tggcgagcta
      121 gaggacggta gagggtggta gaactgctgg tgtagcggtg aaatgcgtag agatcagcaa
      181 gaatacccgt ggcgaaggcg gccatctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006871
VERSION     KHUT01006871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           98 g           37 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtgcccc gtgaaatccc ccggctcaac cggggaacgg cggggcatac tggcggcctt
      121 gagccactta agggcagacg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggcgaaagca gtctgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006872
VERSION     KHUT01006872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcag
       61 caagtctggg gtgaaatcct tcggctcaac cggagaactg ccttggatac tgctgatctc
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006873
VERSION     KHUT01006873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           87 g           51 t
ORIGIN      
        1 tacgtaccgt cctaacgtta ttcggaatta ctgggcttaa agggtacgta ggcggccctg
       61 tcagtcgggt gtgaaatacc tcggctcaac cgaggaactg cgcccgatac tgcagggctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggta cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01006874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006874
VERSION     KHUT01006874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ttgggcgtaa agcgcacgca agcggttgga
       61 caagtccggc gtgaaatctc ccagcttaac tgggagcggt cgcgggaaac tgttcgactt
      121 gaggcgatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc gtcgtgctga cgcacatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006875
VERSION     KHUT01006875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggtggtttga
       61 taagtccgta atgaaatctc ccggcttaac tgggaggggt ttagggatac tgtcaggctt
      121 gagtgtggga gaggtaactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctat ggcgaaagca ggttactggc ccaatactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006876
VERSION     KHUT01006876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagttggat gtgaaagccc tgggcttaac ctaggacgtg catacgagac tggatagcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006877
VERSION     KHUT01006877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           93 g           53 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatga ctgggtgtaa agggagcgta ggcgggatgg
       61 caagtcagat gtgaaacctg agggctcaac cttcagactg catttgaaac tgctgttctt
      121 gagtgaagta gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacatcggt ggcgaaggcg gcttactggg cttttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006878
VERSION     KHUT01006878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           87 g           56 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagtttgta ggcggttgct
       61 taagtctgtc gttaaaggtc ggagctcaac tccggtatgg cgatggaaac tgggcggcta
      121 gagtatgaca gaggtaactg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg ggttactggg tcattactga cgctgaagaa cgaaagccag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01006879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006879
VERSION     KHUT01006879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcggcttcg
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg catccgaaac tacaaagctt
      121 gaatgcttga gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcgga ggcgaaggcg gccaactggc gatgcattga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006880
VERSION     KHUT01006880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           86 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggaactg
       61 taagtctgat ttgaaagtag gtcgcttaac gatctaatgt ggttggaaac tgtggttctt
      121 gaatgtgttg gcggaagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggcttccagg acatgattga cactgaggca cgagagcatg
      241 gggagccaac agg
//
LOCUS       KHUT01006881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006881
VERSION     KHUT01006881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           94 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccat
       61 taagtcggaa gtgaaatctc agggctcaac cctgaaattg ctttcgatac tggcggactt
      121 gaggcaagta ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cttgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006882             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006882
VERSION     KHUT01006882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgcgta ggcggcgtcg
       61 taagtcaggg gtgaaatccc aacggctcaa ccgtggaatt gcctttgata ctgcgatgct
      121 cgagggaggt aggggcaagt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccgg tggcgaaagc gacttgctgg atctcttctg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01006883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006883
VERSION     KHUT01006883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          103 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcac
       61 taagttgccg gtgaaagccc ggggctcaac tccgggaagt ctggtaagac tggtgggctc
      121 gagcacggta gaggcagatg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccgttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006884
VERSION     KHUT01006884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           94 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgtg
       61 taagttggat gtgaaaggcc cgggcttaac ctgggagttg catctgatac tgcatgacta
      121 gagtagtgta gaggggagtg gaatttccag tgtagcggtg aaatgcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg gctccctgga cacatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006885
VERSION     KHUT01006885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          105 g           34 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctggg gtgaaagccc ccggctcaac cggggaaggg cctcagatac tggcggcctg
      121 gaggggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006886
VERSION     KHUT01006886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           96 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaat
       61 tgggtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac taattgactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006887
VERSION     KHUT01006887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatta ctgggcgtaa agggtctgta ggcggttgtt
       61 taggtctgat gtgacacctc tcggctcaac tgagagtatg cattggaaac cggacgacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006888
VERSION     KHUT01006888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          106 g           40 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggcgcgt
       61 taagtggggt gtgaaagccc ggggctcaac cccgggatgc cgctccagac tggcgcgctt
      121 gaggacggaa gaggggcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcgccctggt ccgttactga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006889
VERSION     KHUT01006889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           87 g           47 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgata
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac tgtattgctc
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacgccagg ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006890             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006890
VERSION     KHUT01006890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agggcgcgta ggcggcttag
       61 caagtcggat gtgaaagctc caggctcaac ctggagatgc cacccgatac tgctatggct
      121 agagtccggt aggggaccac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggaactg acgctgaggc gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01006891             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006891
VERSION     KHUT01006891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcggat gtgaaaactc caggctcaac ctggagccgc cattcgagac tgctatggct
      121 agagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccga tggcgaaggc agcactctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006892
VERSION     KHUT01006892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgatac tggcaagcta
      121 gagtgtggta gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctacctgga ccacaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006893
VERSION     KHUT01006893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           87 g           56 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatca ctgggcgtaa agggtctgta ggcggtattt
       61 taggtctgat gtgacaactc ttggcttaac tgagagagtg cattggaaac cggagtacta
      121 gaatcatgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga caatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006894
VERSION     KHUT01006894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggtacgg
       61 taagttggat gtgaaatctc ctggctcaac tgggagaggt cgttcaaaac tgccgaacta
      121 gaggatggta gaggaaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gccttctggg ccatacctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006895
VERSION     KHUT01006895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           86 g           54 t
ORIGIN      
        1 gacgagggat ccaagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctatg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgtagctt
      121 gagtgtagga gaggatagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccgga ggcgaaggcg gctatctggc ctaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006896
VERSION     KHUT01006896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           79 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggctgaaatg
       61 aaagtcagat gtgaaatccc ggggctcaac ctcggaaccg catttgaaac tccatttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgatgcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01006897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006897
VERSION     KHUT01006897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacgtaaggt gcgaacgttg ttcggaatca ctgggcataa agagcacgta ggcggctggg
       61 taagtccaat gtgaaatccc tcggctcaac cgaggaactg catcggatac tgcctggcta
      121 gagtgcagta ggggagagcg gaattccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctgga ctgtaactga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006898
VERSION     KHUT01006898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 cacgtaaggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggttacg
       61 taagcctgat gtaaaaggca ctggctcaac cagtgagttg cgttgggaac tgcatagcta
      121 gagttcagaa gagggaactg gaattccagg tgtaggggtg aaatctgtag atatctggaa
      181 gaacgccaaa gacgaaggta agttcctggt catggactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006899
VERSION     KHUT01006899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcatgca ggcggctctt
       61 taagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tgaagggctt
      121 gaggcagtga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc attggcctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01006900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006900
VERSION     KHUT01006900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggattta ttgggcgtaa agcgcacgta ggcggttttg
       61 taagtctgat gtgaaagccc gtgactcaat cacggaagtg cattggatac tgcaagacta
      121 gagtattgga ggggagaacg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacggt ggcgaaggcg gttctctgga caataactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006901
VERSION     KHUT01006901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggtgtgta ggtggtcaga
       61 taagtcagtg gtgaaatcct acggcttaac cgtagaactg cctttgatac tgtctgacta
      121 gagtgcagga gaggagggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccctctgga ctgtaactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006902
VERSION     KHUT01006902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggttgtg
       61 caagacagat gtgaaatccc cgggcttaac ctgggagctg catttgtgac tgtacggcta
      121 gagtgcggta gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cactcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006903
VERSION     KHUT01006903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcttcg
       61 taggtcggat gtgaaagctc tcggctcaac cgggaaagtg cattcgaaac cacgaagctt
      121 gagtgtcgga gaggagggcg gaattgtcgg tgtagaggtg aaattcgtag atatcgacag
      181 gaacacctgt ggcgaaagcg gccctctgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006904             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006904
VERSION     KHUT01006904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           61 c           90 g           44 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcggggagg
       61 cacgtctgtc gcttaaatcc cacggctcaa ccgtggaact ggcgtagata cgacccccct
      121 ggaggggtgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acactttctg acgctgagac gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006905
VERSION     KHUT01006905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           93 g           42 t
ORIGIN      
        1 gacagggggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggctgccgac
       61 taagtcagat gtgaaagccc ccggctcaac cggggaatag cacctgatac tggttggcta
      121 gaggacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtctctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006906             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006906
VERSION     KHUT01006906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           99 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcgggt gtgaaacctc caggctcaac ttggagcagc cactcgatac tgccatggct
      121 agagtccggt agggggccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggcctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006907             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006907
VERSION     KHUT01006907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggagg ccaggggata ctgcgaggct
      121 tgagtcaagt agaggttggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggccaactgg acttgtactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006908             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006908
VERSION     KHUT01006908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           99 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggccgag
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggagg ccaggggata ctgctcggct
      121 agagacaagt agaggttggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggccaactgg acttgttctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01006909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006909
VERSION     KHUT01006909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttttg
       61 taagtcggat gtgaaatccc cgggcttaac ccgggaactg cattcgagac tgcattactc
      121 gagtatggga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctggc ccaatactga cactcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006910
VERSION     KHUT01006910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggctctg
       61 caagtctgga gtgaaagccc ggggctcaac cccgggactg ctttggaaac tgtggagcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006911
VERSION     KHUT01006911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          101 g           36 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgca ggcggcagag
       61 cgcgtccgcg ctgaaatctg ttggcttaac tgacaggcgc gtgcggatac ggctcagctc
      121 gaggcacgga gagggtgggg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg cccacctggc cgtgacctga cgctgcggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006912
VERSION     KHUT01006912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c          101 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgcg
       61 taagtccggg gtgaaagcct ccagctcaac tggagaacag ccctggatac tgcgggcctc
      121 gaggggggta ggggcgggcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcccgctgga ccctctctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006913
VERSION     KHUT01006913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgca ggcgggtctg
       61 taagtctgtg gtgaaatctc cgagcttaac tcggaaactg ccatggatac tataggtctt
      121 gaatgttgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacacctat tgcgaaggca gcttactacg caaatattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006914
VERSION     KHUT01006914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           79 g           58 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagag gtgaaagacg gtggctcaac catcgcagtg cctttgatac tgaagagctt
      121 gattagacta gaagtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgttaca
      181 gaacaccaat tgcgaaggca gcttactatg gtaagaatga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006915
VERSION     KHUT01006915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggttaaa
       61 caagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgtttgactt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006916
VERSION     KHUT01006916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           91 g           53 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttagct gtgaaattcc tgggctcaac ctagggtggt cagctaagac tgttagactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006917
VERSION     KHUT01006917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           77 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggctttt
       61 taagtcaggg gtgaaatacg gcagctcaac tgtcgcagtg cctttgatac tgaagagctt
      121 gaataacgtt gaagatggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gctgtctaag cgtttattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006918
VERSION     KHUT01006918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcgggtcgt
       61 caagttgggg gtgaaagccc agggctcaac cctggaactg ccttcaaaac tggcgaccta
      121 gagtatggta gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggg ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006919
VERSION     KHUT01006919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           94 g           47 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggtggctacg
       61 taagtcgaat gtgaaagccc tgggctcaac ccaggaagtg catttgatac tgcgtagctt
      121 gagtcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006920
VERSION     KHUT01006920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcac
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaatag ccttggatac tggtggtctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006921
VERSION     KHUT01006921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           93 g           42 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggaatca ctgggcgtaa agcgggtgta ggcggttcct
       61 taagtcagat gtgaaatcct acggctcaac cgtggaactg catctgatac tggggaactt
      121 gaggacggta gaggtcggtg gaattccggg tgtaccggtg aaatgggcag atatccgggg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 gggagcaaac cgg
//
LOCUS       KHUT01006922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006922
VERSION     KHUT01006922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgtgta ggtggtttta
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaatgg cgttgaagac tgtaagactt
      121 gagtaggaca gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatccgt ggcgaaggcg gctatctggg tccaaactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006923
VERSION     KHUT01006923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           84 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgtgta ggcggacgct
       61 taagtagaaa gtgaaaggtt ggtgctcaac accaacattg ctttctatac tgggtgtctt
      121 gagtgccgga gaggaagatg gaactgcacg tgtagcggtg aaatgcgttg agatgtgcag
      181 gaacaccaat ggcgaaggca atcttctaga cggaaactga cgctgagaca cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01006924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006924
VERSION     KHUT01006924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttttg
       61 taagttggct gtgaaattcc tgggcttaac ctggggtggt cagccaatac tgtaagacta
      121 gagtacgaga gagggtagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg actacctggc tcgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006925
VERSION     KHUT01006925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggattta ctaggcgtaa agcgtgggca ggcggttcgg
       61 taagtctgtt gtgaaagctc ctggctcaac tgggagaggc caatggatac tgccggactt
      121 gaatcgggga ggggtcactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaat ggcgaaagca ggtgactgga cccgtattga cgctcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01006926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006926
VERSION     KHUT01006926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctct
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaatag ccttggatac tgggagtctc
      121 gagggaggca ggggcgggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006927
VERSION     KHUT01006927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           99 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggatgga
       61 taagtgggtg gtgaaagcgc tcggctaaac cgggcgagtg ccattcagac tgtctatcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaagca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006928             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006928
VERSION     KHUT01006928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           96 g           45 t
ORIGIN      
        1 tacgagggga gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcctgc
       61 acaagtccag ggtgaaagcc ctcggctcaa ccgaggaagg gccttggaaa ctgtgcggct
      121 tgagtattgg agaggaaggc ggaattccca gtgtagcggt gaaatgcgta gatattggga
      181 ggaacacctg tggcgaaggc ggctttctgg acaattactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01006929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006929
VERSION     KHUT01006929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttatt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg ctttcgatac tgggtaacta
      121 gagtatgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggc ttaatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006930
VERSION     KHUT01006930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgc
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg catccgatac tggccgactc
      121 gagtgcgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006931
VERSION     KHUT01006931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           97 g           46 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgact gtgaaaaccc acggctcaac cgtgggcctg cagtcgatac gggcaagctc
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006932
VERSION     KHUT01006932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           87 g           51 t
ORIGIN      
        1 gacgagctct ccaaacgtta ttcggtatca ctgggcttaa agagttcgta ggcggtcctg
       61 taggtggggt gtgaaatccc tcggctcaac cgaggaattg cgcctcaaac cccgggactt
      121 gagggaggca gaggtaagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006933
VERSION     KHUT01006933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           77 g           60 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaagcct gaagctcaac ttcagaattg ccattgatac tgaagagctt
      121 gagtatggtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006934             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006934
VERSION     KHUT01006934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           30 c           70 g           71 t
ORIGIN      
        1 tacgggaagg gcaagcgtta tccggcgtga ctgggcgtaa agagtccgta gacggtagag
       61 taagtttttt gttaaatttt aaaacttaat tttaagaaag caattaaaat actgctttac
      121 tttgagttca gtgcagaaaa gtagaatttt atatgatagg ggtgaaatcc gtagatatat
      181 agaggaatgc caatagcgaa ggcaactttt tggtatgaac tgacgttgag ggacgaaagt
      241 gtgggtagca aacagg
//
LOCUS       KHUT01006935             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006935
VERSION     KHUT01006935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaggta ggtggtctga
       61 tttgtctggc ggtgaaatcc ttgagcttaa ctcaagaagt gccccggaaa cggtcagact
      121 tacgagtctt ggagagggtg gcggaattcc cggtgtagcg gtgaaatgcg tagagatcgg
      181 gaggaacacc agaggcgaag gcggccacct ggacaagcac tgacactcaa ctgcgaaagc
      241 gtggggagca aacagg
//
LOCUS       KHUT01006936             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006936
VERSION     KHUT01006936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           43 c           75 g           56 t
ORIGIN      
        1 tacgggagat gcaagcgtta tccggaatga ttgggcgtaa agcgtttgaa caggtggttt
       61 atgaaagtcg attgttaaaa cccaaggctt aaccttggac cggcaatgga aacttctaaa
      121 ctagagtttg gtagaggtag acggaattac cggtgtaacg gtgaaatgtg cagatatcgg
      181 taagaactcc aatggcgaaa gcagtctact gggccacaaa ctgacactta agaacgaaag
      241 ctaggggagc gaatagg
//
LOCUS       KHUT01006937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006937
VERSION     KHUT01006937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccaag
       61 tatgtcggat gtgaaatccc gcggctcaac cgtggaagtg cattcgaaac tgcttggctg
      121 gagtttcgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacacctgt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006938
VERSION     KHUT01006938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           73 g           60 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agagctggta ggtggttata
       61 ttagtcagat gtcaaatctt cgagctcaac tcgaaaactg catttgaaac ggtataacta
      121 gagggtgtga gagatctatg gaactcatgg tgtagcagtg aaatgcgttg atatcatgag
      181 gaacaccaaa ggcgaaggca ttagattggc acactcctga cactgagcag cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01006939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006939
VERSION     KHUT01006939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ttgggcttaa agggcgtgta ggcggcggcg
       61 caagcgtcgt gtgaaatccc atcgctcaac gatggaactg cacggcgaac tgcgtcgctt
      121 gaggtcggta gaggtgcttg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcctga ggcgaaagcg gagcactggg ccgattctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006940
VERSION     KHUT01006940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgtacgca ggctgtccgt
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaattg cgcccgaaac tgacgggctt
      121 gagtgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006941
VERSION     KHUT01006941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggtcggc
       61 taagtccgat gtgaaatccc ccggctcaac cggggaactg catcggatac tggctgactt
      121 gagagttgta ggggtggacg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccgga ggcgaaagcg gtccactggg cagtctctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006942
VERSION     KHUT01006942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacagagacg tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagtcggat gtgaaatccc agagcttaac tctggaagtg catccgaaac tgccttgctc
      121 gaggactgga ggggagactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006943
VERSION     KHUT01006943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           87 g           46 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtccga
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggc caacggaaac tatcgggctt
      121 gagtatggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006944
VERSION     KHUT01006944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctgcg
       61 taagtcgggc gtgaaatccc acggctcaac tgtggaagag cactcgaaac tgcgtagcta
      121 gagtcctgga gaggaaggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006945
VERSION     KHUT01006945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           80 g           60 t
ORIGIN      
        1 tacagagagt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatatt
       61 taagttggat gtgaaatccc tgggcttaac ctaggaattg cattcaatac tggatgtcta
      121 gagtatagaa gagggaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg gcttcctggt ctaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006946
VERSION     KHUT01006946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           38 c           85 g           59 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggtttga
       61 taagttggga gtgaaatccc ggggcttaac ctcggaattg ctttcaaaac tattaagcta
      121 gagtatggta ggggatagtg gaattcttag tgtaggggtg aaatccgtag atattaagag
      181 gaacaccaga ggcgaaggcg actatctggg ccattactga cgctaaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006947
VERSION     KHUT01006947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtccttt gtgaaagctc ccggcttaac tgggagaggt caagggatac taccgagcta
      121 gagtgtggta gaggatactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggtatctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01006948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006948
VERSION     KHUT01006948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggtttac
       61 taagttggac gtgaaagctc ccggcttaac tgggagaggt cgttcaaaac tggaaaactt
      121 gagtctggaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt ccagaactga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006949
VERSION     KHUT01006949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtcggtc gtgaaaggct tcggctcaac cggagatggc cgatcgaaac tgccgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cgacctctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01006950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006950
VERSION     KHUT01006950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           87 g           54 t
ORIGIN      
        1 cacgtaggag gcgagcgtta ttcggattta ctgggcgtaa agcgcgtgta ggcggcttta
       61 taagttggat gtgaaagctc ccggcttaac tgggagagga cgttcaaaac tatagagctt
      121 gagtgtgaga gaggaaaatg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg attttctggc tcaagactga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01006951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006951
VERSION     KHUT01006951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgagagcc tcgagcgtta tccggaatta ttgggcgtaa agggtgcgta ggttgttctg
       61 ttagtctcct gttaaagccc cgggcttaac ctgggatgag cgggggaaac ggcagaacta
      121 gaaagtgcga gaggtgtacg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa ggcgaaagca gtacactggc gcatatttga cactgaggca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01006952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU6999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006952
VERSION     KHUT01006952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU6999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           83 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgt
       61 taagtcaggt gtgaaatccc cgggctcaac ctaggaattg catttgaaac taacaaacta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccattactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006953
VERSION     KHUT01006953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtttgt
       61 taagcaggat gtgaaatccc cgggctcaac ctgggaactg catcctgaac tggcaggcta
      121 gaatgcagta gaggatagtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatcgat ggcgaaggca gctatctgga ctgacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006954
VERSION     KHUT01006954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtttgg
       61 tgggtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac taccgaactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006955
VERSION     KHUT01006955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           93 g           46 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatca ttgggcgtaa agcgcatgta ggcggcccgg
       61 taagtcggat gtgaaatccc agggctcaac cctggaactg catccgatac tgctgggctt
      121 gaggaaggta gaggagagtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg cctctcctga cgctgagatg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006956
VERSION     KHUT01006956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgg
       61 tcagttaggg gtgaaatccc ggagctcaac tccggaactg cctttaatac tgccagtcta
      121 gagaccgaga gaggtaagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactggc tcggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006957
VERSION     KHUT01006957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           92 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcgggtttg
       61 taagtcagag gtgaaatccc gcagcttaac tgtggaactg cctttgatac tgcaagcctt
      121 gagtctcgga gaggtagctg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga ggcgtaggcg ggctactgga cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006958
VERSION     KHUT01006958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           82 g           58 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatgct
       61 taagtcggtt gtgaaatccc cgagcttaac ttgggaattg cattcgatac tgggcgtcta
      121 gaatatggta gagggaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccaatattga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006959
VERSION     KHUT01006959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           98 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgcgt
       61 caagtcaggg gtgaaaggcc tgggctcaac ctgggaactg cctttgatac tgacgtgctg
      121 gagtacggga gagggtgacg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gtcacctggc ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006960
VERSION     KHUT01006960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           73 g           63 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggacttt
       61 taagtcagtg gtgaaatctt acagcttaac tgtaaaacag ccattgaaac tgatagtctt
      121 gagtatggtt gaggtggatg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccgat agcgaaggca attcactaaa ccattactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006961
VERSION     KHUT01006961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacgtaggct ccaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtccgt
       61 caagtcggct gtgaaatctc ctggctcaac tgggagggtg cggccgaaac tgacggacta
      121 gagttcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgaagacg gctctctgga ccgatactga cgctgagact cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01006962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006962
VERSION     KHUT01006962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           75 g           62 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaaaaa
       61 taagtcagtg gtgaaaacct gcagcttaac tgtagaactg ccattgatac tgttattctt
      121 gagtatggtc aaggtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gcttactgga ccattactga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006963
VERSION     KHUT01006963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           75 g           57 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtgcgca ggcggtactg
       61 taagtcagtg gtgaaatctc tcggctcaac cgagaaactg ccattgatac tgcagtactt
      121 gaatacagtt gaagtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat agcgaaggca gcttactaaa ctgatattga cgctcatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006964
VERSION     KHUT01006964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           97 g           39 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccctg
       61 taagtcccgc gtgaaagccc tcggctcaac cgaggaactg cgcgggagac tgcaaggctt
      121 gagttcggga gagggaagtg gaattccggg tgtagcggtg aaatgcgcag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006965
VERSION     KHUT01006965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           85 g           49 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgtacgta ggcggattcg
       61 caagtcgggc gtgaaatccc cgggctcaac ccgggaactg cgttcgaaac tgcgtatctc
      121 gagtaatgga gagggaatcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg gattcctgga catttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006966
VERSION     KHUT01006966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           83 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggccgcgcgg
       61 gcagtcaaat gtgaaattct gtggctcaac ctcagaattg cgcttgatac ttccgcgctc
      121 gaaccttgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacactaag ggcgaaggca agactctaga caagtgttga cgctgatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006967
VERSION     KHUT01006967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggtggcgggc
       61 taagtctggt gtgaaagctc tgagcttaac tcagaaatgg cactagatac tagtttgctt
      121 gaggattgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006968
VERSION     KHUT01006968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           90 g           55 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggactaa
       61 taagttggtg gtgaaatccc gaggctcaac ctcgggtctg ctgccaatac tgttagtctt
      121 gagttatgga ggggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca gctttctggc catatactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01006969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006969
VERSION     KHUT01006969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          103 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggga
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccatgcggac tgtcttgctt
      121 gagcgtagta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctactgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006970
VERSION     KHUT01006970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ttgggcttaa agggtgcgta ggcgggttgc
       61 taagtgttct gtgaaatccc tcggctcaac cggggaatcg cagggcatac tggcagcctt
      121 gaggcatgta ggggcggacg gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg gtccgctggg catgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006971
VERSION     KHUT01006971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggaatga
       61 taagtcagtg gtgaaagctc cgagctcaac ttggaaattg ccattgatac tgttgttctt
      121 gagttcggtt gaggtaggcg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccgat tgcgaaggca gcttaccaag ccgacactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01006972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006972
VERSION     KHUT01006972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           88 g           46 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtcaggt gtgaaagccc ggagcttaac tccggaattg cgcctgaaac tacatggcta
      121 gagcattaga gagggtagcg gaattcacgg tgtagcagtg aaatgcgcag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctacctgga taattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01006973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006973
VERSION     KHUT01006973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 tacgagtggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggatgtc
       61 caagtgttta gtgaaatccc ccggctcaac cggggaatag ctgggcaaac tggatgtctt
      121 gaggcaggta gaggcagacg gaactgtcgg tggagcggtg aaatgcattg agatcgacgg
      181 gaacgccaaa ggtgaagaca gtctgctggg cctgttctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006974
VERSION     KHUT01006974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           85 g           56 t
ORIGIN      
        1 tacggaaggt gcgagcgtta atcggattta ctgggcgtaa agcgcgcgta ggtggttttg
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaattg catctgatac tgcatgacta
      121 gagtatgcta gagagaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcaat ggcgaaggca acttcttggg gcaatactga cactgaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006975
VERSION     KHUT01006975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggccgtccgc
       61 taagtctgat gtgaaatacc ccggctcaac cggggaactg catctgatac tggcggactt
      121 gaggatggtt ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccatctctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006976
VERSION     KHUT01006976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           85 g           49 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgtcctgt
       61 cacgtctggc agtaaattgc gaggctcaac ctcgcaggtc tgttggaaac gagcaggatg
      121 gagtcactca gaggcatctg gaatgtcatg tgtaggggta aaatccgtag atacatgatg
      181 gaacgccaaa agcgaaagca ggatgctagg ggtgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01006977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006977
VERSION     KHUT01006977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           90 g           47 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggcctgg
       61 caagtcgggc atgaaatctc tcggctcaac cgagagaggc tgtccgatac tgctgggctt
      121 gaggacggta gagggtggtg gaattccgcg tgtagtggtg aaatgcgtag atatgcggag
      181 gaacaccagt ggcgaaggcg gccacctgga ccgtccctga cgctcaaaca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01006978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006978
VERSION     KHUT01006978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           54 c          101 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttcgt
       61 tcagtccgtt gtgaaagctc ccggctcaac tgggagaggt cttcggatac tggcggactc
      121 gagggaggca gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctttcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006979
VERSION     KHUT01006979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ttgggcttaa agggtatgta ggcggcgctg
       61 ttagtcgggt gtgaaatgcc tcggctcaac cgaggaattg cgcccgatac tgcagtgcta
      121 gaggaagaca gaggcaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttgctggg tcttttctga cgctgagata cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01006980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006980
VERSION     KHUT01006980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 tacagaggtg acgaacgttg tccggaatca ctgggcgtaa agggcacgta ggcggttcgc
       61 taagtcagat gtgaaatcca ggagcttaac tcttgaattg catttgaaac tggcgtgcta
      121 gagttcggta gaggaaggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gccttctggt ccgatactga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006981
VERSION     KHUT01006981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 tacagaggcc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcattg
       61 taagtcagat gtgaaatctt ggggctcaac cccgagactg catccgatac tgcagtgctt
      121 gaggattgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga caatacctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006982
VERSION     KHUT01006982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtccag
       61 taagtcgatt gtgaaagccc tcggcttaac cgaggaagtg cagtcgaaac tgctggactt
      121 gaatactgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006983
VERSION     KHUT01006983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           96 g           41 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccaag
       61 caagtcgggt gtgaaagccc ggggctcaac cccggaagtg cactcgaaac tgcatggctg
      121 gagtctcgga gagggaagcg gaattctggg tgtagaggtg aaattcgtag atatccagag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgagtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006984
VERSION     KHUT01006984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           90 g           43 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatggc
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaaccg ctgggcatac tggtcgtctt
      121 gagccaccta ggggcaactg gaacgaacgg tggagcggtg aaatgcgtag atatcgttcg
      181 gaacgccaac ggtgaaaaca ggttgctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006985             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006985
VERSION     KHUT01006985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           50 c           80 g           54 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggttt
       61 tacaagtcag aggtgaaatc ccaaggctca actttgggac tgtcctttga aactgtacaa
      121 ctagaggggc aaagaggaag ctggaacgaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 tcggaacacc aatagcgtag gcaggcttct gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01006986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006986
VERSION     KHUT01006986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          100 g           44 t
ORIGIN      
        1 tacggaggtg gcgagcgttg ttcggaatca ttgggcttaa agggcgtgta ggcgggatgc
       61 caagtgtcgg gtgaaagccc cgggctcaac ccgggaatgg ctcggcatac tggcatcctt
      121 gaggcaggca gaggtcactg gaacgactgg tggagcggtg aaatgcgtag atatcagttg
      181 gaacgccgga ggcgaaagcg ggtgactggg cctgtcctga cgctgatacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01006987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006987
VERSION     KHUT01006987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           74 g           65 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggttta
       61 taagtcagtg gtgaaatccg gcagctcaac tgtcgcactg ccattgatac tgtaagtctt
      121 gaattactgt gaagtggtta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca gatcactaac agtcaattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01006988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006988
VERSION     KHUT01006988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           86 g           51 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggccaag
       61 caagttagaa gtgaaagccc agggctcaac cttggaattg cttttaaaac tgctaggcta
      121 gaatctcgga gaggatagcg gaatttccag tgtagaggtg aaattcgtag atattggaag
      181 gaacaccagt ggcgaaggcg gctatctgga cgagtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01006989             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006989
VERSION     KHUT01006989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           75 g           60 t
ORIGIN      
        1 tacgagaggg gcgagcgtta ttcggaatga ttgggcgtaa agcgcacgta ggctgtttag
       61 aaaatcaggg ataaaaaact caaagcttta ctttgaaact tatcttaatg attcctaaac
      121 tcgagtgagc tagaggagaa tggaattcct ggcgtagagg taaaatttgt tgatatcagg
      181 aggaacacca aaagcgaagg caattctctg gggcttcact gacgttgagg tgcgacagcg
      241 tggggatcaa aaggg
//
LOCUS       KHUT01006990             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006990
VERSION     KHUT01006990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           36 c           82 g           60 t
ORIGIN      
        1 tacgagaggg gcgagcgtta cccggattaa ttgggcgtaa agggtccgta ggcggtattt
       61 tgtatgcttt tggtaaaagg caagggctta actcttggaa tgccttaaga acagatatac
      121 tagagtatag tagaggatag tagaattcca tgtggagggg tggaatccta agatatatgg
      181 aggaatgcca gtagcgaaag cgactatctg gactattact gacgttgagg gacgaaagcg
      241 tagggagcaa acagg
//
LOCUS       KHUT01006991             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006991
VERSION     KHUT01006991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           94 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggaagg
       61 cacgtctgtc gctgaaatcc cccggcttaa ccggggaacg ggcgtagata cgaccttcct
      121 ggaggaacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01006992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006992
VERSION     KHUT01006992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           99 g           35 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctctg
       61 cgcgtggggt gtgaaagcgg acggcttaac tgtccgaggc caccgcaaac ggcagagctc
      121 gagccatgca gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg catgtgctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01006993             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006993
VERSION     KHUT01006993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           36 c           78 g           62 t
ORIGIN      
        1 gacataggtc gcaaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggttcag
       61 taagttactg gtgggaaatc gaggctcaac ctcgtggaag ttagtaatac tgttgaactt
      121 gagtgcagga gaggttaacg gaatggttcg tggaggggta aaatccatag atatgaactg
      181 gaacatcaat atggcgaaag cagttaacta ttctgttact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01006994             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006994
VERSION     KHUT01006994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           82 g           60 t
ORIGIN      
        1 tacgaaggct ccaagcgtta tccggattta ttgggcgtaa agcgtgcgca ggtggtttga
       61 tgcgtcttgt gttaaatctc agtgcctaac attgaggctg catgggatac ggtcaaacta
      121 gaggatgtga gaggctactg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca ggtagctggc acatttcctg acactcattg cacgaaagct
      241 agggtcgcga atggg
//
LOCUS       KHUT01006995             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006995
VERSION     KHUT01006995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           77 g           60 t
ORIGIN      
        1 gacataggtt gcaaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtttga
       61 taagttatca gtggcaaatc ggggctcaac ctcgtggaag ctgataatac tgtcatactt
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgcgttg atatatggaa
      181 gaacaccgat aaggcgaagg cagttaacta ttctgctact gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01006996             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006996
VERSION     KHUT01006996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggtggtgtgt
       61 taagtctgcc ggtcaaatgc cgaggctcaa cctcgttgca cgccggggat actggcacgc
      121 tagagacaag tagaggcaag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacaccg gaggcgaagg cggcttgctg ggcttggtct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01006997             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006997
VERSION     KHUT01006997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          105 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctcg
       61 taaggctggt gtgaaaggtt cagggctcaa cccgaacagg gcactggcga ctgggagcct
      121 tgagtggggg agaggggagc ggaattccgg gtgtagcggt gaaatgcgta gatatccgga
      181 ggaacaccga tggcgaaggc agctctctgg cctctttact gacgctgagg tgcgaaagcg
      241 tggggagcga acggg
//
LOCUS       KHUT01006998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006998
VERSION     KHUT01006998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctc
       61 caagtcggtg gtgaaatctt tcagctcaac tggaaaagtg ccgccgatac tggggatctc
      121 gagggaggta ggggtcgatg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01006999             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01006999
VERSION     KHUT01006999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           66 c          113 g           29 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccacc
       61 gcaaggcccg tgtgaaagcc cggggctcaa ccccgggtcg gcgcgggcga ctgcggggct
      121 agagggcgcg gtagaggccg gtggaattcc gggtgtagcg gtggaatgcg tagagatccg
      181 gaagaacacc agtggcgaag gcggccggct gggccgcggc cgacgctgag gcgcgacagc
      241 gtggggagca aacagg
//
LOCUS       KHUT01007000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007000
VERSION     KHUT01007000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           88 g           53 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggattta ctgggcgtaa agggcgtgta ggtggtaaag
       61 caagtaagat gtgaaatccc tgggcttaac ctaggaagtg catcttaaac tgcttaacta
      121 gagtacggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgccagt ggtgaagacg actacctgga tcgatactga cactgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007001
VERSION     KHUT01007001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           88 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agagttcgta ggtggttagg
       61 taagttatgt gtgaaatccc tgggcttaac ctgggcaggt catataatac tgcttgactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007002
VERSION     KHUT01007002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           74 g           60 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaatga
       61 taagtcagtg gtgaaatcct gcagcttaac tgcagaactg ccattgatac tgtcgttctt
      121 gaatacaact gatgtgggcg gaatgtgcca tgtagtggtg aaatacttag atatggcaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ttgttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007003
VERSION     KHUT01007003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctca
       61 tttgtcaggg gtgaaagcct tgggcttaac tcaagaagtg cccctgaaac ggtgagacta
      121 gagtgctcga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga gagtgactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007004
VERSION     KHUT01007004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           85 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggtggtttaa
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg catttgaaac tattaggcta
      121 gagtacggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccgcaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007005
VERSION     KHUT01007005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           76 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggacatt
       61 taagtctggg gtgaaatctc cgagctcaac tcggaaactg ccttggatac tatttgtctt
      121 gaattatgtt gaggtaggcg gaatataaca tgtagcggtg aaatgcatag atatgttata
      181 gaacaccgat tgcgaaggca gcttactaag catatattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007006
VERSION     KHUT01007006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           86 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggcggattgt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tggctttctt
      121 gagttcggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgaaactga cgctgatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007007
VERSION     KHUT01007007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           99 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttgga
       61 taagttgcgt gtgaaagctc ctggctaaac taggagagga caggcaagac tgttcggcta
      121 gagggtggta gaggggcaca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggtgaagacg gtgccctggg ccatacctga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007008
VERSION     KHUT01007008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tgctttgctt
      121 gagtaccgga gaggatggcg gaatgcccca agtagaggtg aaattcgtag atatggggcg
      181 gaacaccggt ggcgaaggcg gccatctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007009
VERSION     KHUT01007009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c          100 g           47 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ttgggcgtaa agggagcgta ggcggtgcgg
       61 taagtcgggt gtgaaacctc caggctcaac ttggagctgg catccgaaac tgccgtgcta
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcggt ggcgaaggcg gctctctgga ccataactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007010
VERSION     KHUT01007010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           99 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtgaga
       61 agggtgtgat gtgaaagccc cgggcttaac ctgggaaggg catcgcaaac catctgactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007011
VERSION     KHUT01007011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           98 g           50 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtcttg
       61 tatgtcttcc gtgaaagccc acagctcaac tgtggtgtgt cgggagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007012
VERSION     KHUT01007012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           88 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaccac
       61 caagtcggac gtgaaagccc tgagctcaac tcaggaactg catccgaaac tggcggtctg
      121 gagtagtgga gaggaaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cacttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007013
VERSION     KHUT01007013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           83 g           47 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaaagg
       61 aaagtcagat gtgaaatccc ggggctcaac ctcggaacag catttgaaac tcccattcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01007014             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007014
VERSION     KHUT01007014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           82 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatta ttgggcgtaa agcgatcaca ggtggacaag
       61 taggtctggt gttaaatacc cacggctcaa ccggggtgct gcattggaaa cctcttgact
      121 agaggaaggc aaaggcaggt ggaatttcca ttgtaggggt taaatccgta gataatggaa
      181 ggaacaccga aagcgaaggc agcctgctgg gcctttcctg acactgcttg atcgaaagcg
      241 tggggagcaa aaggg
//
LOCUS       KHUT01007015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007015
VERSION     KHUT01007015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagttgggt gtgaaagctc ggagcttaac tccgaaacgg cattcaaaac tacttggctg
      121 gagggtcgga agggagactg gaattctcgg tgtagcggtg aaatgcgtag agatcgagag
      181 gaacaccagt ggcgaaagcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007016
VERSION     KHUT01007016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggccttc
       61 caagtctggt gtgaaatctc ccggcttaac cgggagggtg cgccggaaac tggggggctc
      121 gagtgtggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagacg gctttctgga ccacgactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007017
VERSION     KHUT01007017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c          100 g           38 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggcggtgagg
       61 taagtggctg gtgaaagcct tcggcttaac cgagggagtg ccggtcagac tgccccacta
      121 gagtactgga gagggacgcg gaattccggg tgtagcggtg aaatgtgcag agatccggag
      181 gaacaccagc gccgaaaggg gcgtcctgga cagaaactga cgctgagacg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007018             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007018
VERSION     KHUT01007018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           46 c           98 g           53 t
ORIGIN      
        1 tacggggggg gctagcgttg ttcggattta ctgggcgtaa agggagcgta ggcggcttgt
       61 caagtccggt gtgaaatctt taccgcttac cggaaagagg tcatcggaaa ctggcaggct
      121 agagcagggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatctg tggcgaaggc ggcttgctgg gctcttgctg acgctgaggc tcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007019             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007019
VERSION     KHUT01007019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctagg
       61 taagtccggt gtttaatctt ggggctcaac cccaagtcgc acgggaaact gccaggcttg
      121 agtgcagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007020             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007020
VERSION     KHUT01007020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           85 g           55 t
ORIGIN      
        1 tacgtatggg gcaagcgtta tccggaatta ttgggcgtaa agagtgcgta ggtggttacc
       61 taagcgcaag gtttaatctt gaggctcaac ctcattctgc cttgcgaact gggctacttg
      121 agtgcaggag gggaaagcgg aattcccagt gtagcggtga aatgcgtaga tattgggaag
      181 aacaccggtg gcgaaggcgg ctttctggac tgtaactgac actgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007021             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007021
VERSION     KHUT01007021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttctt
       61 taagtcttgt gtttaagccc gtggctcaac cacggtccgc atgggaaact ggggaactgg
      121 agtgcaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007022             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007022
VERSION     KHUT01007022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           79 g           61 t
ORIGIN      
        1 tacggaggat tcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggttaga
       61 taagtttgag gttaaatgct tctgctcaac aggagtttgc cttgaaaact gtctgactag
      121 gatgtaaacg gcgcaggcgg aatgaatcat gtagcggtga catgcataga tatgattcag
      181 aacaccgatc gcgaaggcag cttgcgagga tacgattgac gctgaagcac gaaagcgtgg
      241 gtatcgaaca gg
//
LOCUS       KHUT01007023             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007023
VERSION     KHUT01007023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ttgggcgtaa agggagcgta ggcggcgggg
       61 taagtcaggt gttaaaattc ggcgctcaac gccgaagcgc atttgatact gctctgctag
      121 aggagtggag aggagacagg aattcacggt gtagcagtga aatgcgtaga tatcgtgagg
      181 aagacccatg gcgaaagcat gtctctggaa actacctgac gctgatgctc gaaggccagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01007024             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007024
VERSION     KHUT01007024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           37 c           77 g           55 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggtctaa ctaggcgtaa agggtccgta ggcggttaaa
       61 aaagttgaaa gcaaaagcca aaggcttaac ctttgaaaga ttttcaaaac tatttaacta
      121 gagtattaca ggggatagtg gaatttctgt agtagggatg gaatccattg ataacagaag
      181 gaacaccagt ggcgaaggcg actatctggg taatactgac gctgagggac gaaggcatgg
      241 ggagcaaatg gg
//
LOCUS       KHUT01007025             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007025
VERSION     KHUT01007025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           86 g           57 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggttcgca ggcggttttt
       61 taagtcagat gtgaaaggct acggctcaac cgtggtaagc atttgaaact gaaagacttg
      121 agttaaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aataccagtg gcgaaggcga ctttctggac ttatactgac gctgaggaac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007026             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007026
VERSION     KHUT01007026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttctt
       61 taagtcttgt gtttaatccc agagctcaac tctggtacgc atgggaaact gggggactgg
      121 agtgcaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007027             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007027
VERSION     KHUT01007027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcgtt
       61 taagtccgat gtttaaaccg agggctcaac cctcggtcgc atgggaaact gggcgactgg
      121 agtgcaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007028             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007028
VERSION     KHUT01007028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtgcgta ggcggccagg
       61 taagttggat gtcaaagcca gcggctcaac cgctgtccgc atccaaaact gtctggcttg
      121 agtgcaggag aggagagtgg aattcccggt gtagcggtgg aatgcgtaga gatcgggagg
      181 aacaccagtg gcgaaggcgg ctctctggcc tgttactgac gctgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01007029             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007029
VERSION     KHUT01007029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           74 g           61 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gacggtaaaa
       61 aaagttacta gttaacgtcg agctcaactt gactgaaatt agtaatacta ttttgctaga
      121 gaatggaaga ggttgatgga agtctatgag gaggggtgaa atccgttgat ccatagggta
      181 ccaccaaaag gcgaaggcaa ttaactattc catttctgac gttgaggcat gaaagcgtgg
      241 ggagcaaacc gg
//
LOCUS       KHUT01007030             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007030
VERSION     KHUT01007030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           92 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgtgta ggcggttcgg
       61 taggtctcgt gtcaaatcct gcggctcaac cgcagtccgc acgggaaacc accttactag
      121 agtccggaag aggagagcgg aattccaggt gtagcggtga aatgcgcaga tatctggaag
      181 aacaccagtg gcgaaggcgg ctctctggga cggtactgac gctgagacgc gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01007031             260 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007031
VERSION     KHUT01007031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>260
                     /product="16S ribosomal RNA"
BASE COUNT           84 a           37 c           75 g           64 t
ORIGIN      
        1 tacgaggggg gcaagcgtta ttcggaatga ttgggcgtaa agggcatcta ggcggtttag
       61 tactattaaa tatcaaacaa aatcccagag cttaactgtg gtgattattt aaacttgtac
      121 tttactagag taagtgagag gaaaatggaa tttctggagg agaggtaaaa tttgtagata
      181 tcagaaggaa catcgaaagc gaaggcaatt ttctggcact taactgacgc tgacgtgcga
      241 aagcgtgggg agcaaccggg
//
LOCUS       KHUT01007032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007032
VERSION     KHUT01007032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           83 g           58 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctaat
       61 taagtcaggt gtgaaatccc agggctcaac cctggacgtg catttgatac tgattagctt
      121 gagtgtggga gaggttacta gaattcttgg tgtagtggtg aaatacgtag atatcaagag
      181 gaataccggt ggcgaaggcg ggtaactggc ccaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007033             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007033
VERSION     KHUT01007033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 aaaagtcagg tgtgaaatct cggggctcaa cctcgaaact gcacttgata ctgccctgct
      121 cgagtcctgg agaggagact ggaatttacg gtgtagcagt gaaatgcgta gagatcgtaa
      181 ggaagaccag tggcgaaggc gagtctctgg acaggtactg acgctgaggc acgaaggcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007034             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007034
VERSION     KHUT01007034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           78 g           60 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gacggtaaaa
       61 taagttatta gttaacgcca agctcaactt ggctgaagct agtaatactg ctttgctaga
      121 ggatgggaga ggttgatgga agtctatgag gaggggtgaa atccgttgat ccatagggta
      181 ccaccaaaaa aggcgaaggc aattaactat tccatttctg acgttgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01007035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007035
VERSION     KHUT01007035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           96 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaattg ccttggatac tggtggtctt
      121 gagggaagta ggggtctgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcggactggg cttcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007036
VERSION     KHUT01007036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggtttgg
       61 taagtcaggg gtgaaactcc agagctcaac tctgggcgtg cctctgaaac tgctagactt
      121 gagtgctcta gagggtagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg actacctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01007037             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007037
VERSION     KHUT01007037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           51 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgg
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctgtcgagct
      121 ggagtgatgc agaggtttgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcaaactgg gcatcaactg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007038             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007038
VERSION     KHUT01007038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           81 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggactat
       61 ttaagtccgt ggtgaaatct ccgggctcaa cccggaaact gccatggata ctattagtct
      121 tgaatattgt tgaggtgggc ggaacgggtc atgtagcggt gaaatgcata gatatgaccc
      181 ggaacaccga ttgcgaaggc agcccactaa gcaatgattg acgctgaggc acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01007039             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007039
VERSION     KHUT01007039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           86 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggtggga
       61 taagtcagtt gtgaaattcc gtggcttaac catagggact gcgattgata ctgtttcact
      121 tgaatcaggt tgaggtgagc ggaatgtggc atgtagcggt gaaatgcata gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg acctgtattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007040
VERSION     KHUT01007040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           90 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcgccg
       61 caagtcaggc gtgaaatccc cgagctcaac ttgggaattg cgcttgaaac tacggtgctg
      121 gagtatggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007041
VERSION     KHUT01007041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtcggat gtgaaagctc gaagcttaac ttcgaaatgg cattggaaac taccttgctt
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007042
VERSION     KHUT01007042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccttg
       61 caagcgatgg gtgaaatccc tcggcttaac cgaggaactg cctgtcgaac tgcatggctc
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007043
VERSION     KHUT01007043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgcac
       61 taagttcgat gtgaaagccc tgggctcaac ccaggaactg cattgaaaac tggtgggctt
      121 gagttcggga gagggtcgcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgacctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007044             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007044
VERSION     KHUT01007044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           80 g           54 t
ORIGIN      
        1 tcccagcagt ccaagtggca gccgctttta ttgggtctaa aacatccgta gcttgcttcg
       61 taagtctctt gtgaaatccg gcctcttaag ggtcggtcgc gcaagagaga ctgcgttgct
      121 agagaccggg agacgtaaag agtacgcatc gggtagcggt aaaatgcgtt gatccaatgt
      181 ggactaacga tagcgaaggc actttacgag aacggatctg acagtgaggg atgaaggcta
      241 ggggcgcaaa aggg
//
LOCUS       KHUT01007045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007045
VERSION     KHUT01007045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggatatt
       61 taagtcagat gtgaaatccc tgggctcaac ccgggaatgg catttgaaac tggatatcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007046
VERSION     KHUT01007046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 tacggggggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccgga
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgatac tgtttggctc
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007047
VERSION     KHUT01007047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           96 g           37 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggctcg
       61 tgagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac cgcgggccta
      121 gagggggaca ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgtgctgga tccctactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007048             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007048
VERSION     KHUT01007048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatt
       61 taagtcaggt gtgaaagccc cgagcttaac ttgggaatgg cgcttgagac tggatagctt
      121 ggagtgtggc agagggaggt ggaattccac gtgtagcagt gaaatgcgta gagatgtgga
      181 ggaacaccga tggcgaaggc agcctcctgg gccagcactg acgctcatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007049
VERSION     KHUT01007049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggagggcagg
       61 taagtcagtg gtgaaatccc cgtgcttaac atgggaactg ccgttgatac tatctgtctt
      121 gaatgccgtg gagatgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgttaca cgaacattga ctctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007050
VERSION     KHUT01007050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           99 g           46 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggtacga
       61 taagtctgct gtgaaagctc cccgcttaac gaggagaggt cggtggatac tgtcgcactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007051
VERSION     KHUT01007051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           99 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccctg
       61 gaagtcgggt gtgaaagccc ggggctcaac cccggaacgg cacccgatac tcctgggctc
      121 gagaccggaa gaggtgagtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007052
VERSION     KHUT01007052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacgtatgga gcaagcgtta tccggattta ctgggtgtaa agggagtgta ggtggccata
       61 caagtcagaa gtgaaagccc ggggctcaac cccgggactg cttttgaaac tgtaaggctt
      121 gagtgcagga ggggtgagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctcactgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007053
VERSION     KHUT01007053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           86 g           57 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ttgggcgtaa agagttcgta ggcggtgtgc
       61 caagtttggt gtgaaatctc ccggctcaac tgggaggctg catcgaatac tggcatgctc
      121 gagtatagga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ctataactga cgctgaggaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007054
VERSION     KHUT01007054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 taagtcgggt gtgaaagccc cgggcttaac ttgggaatgg cattcgatac tgtcaagcta
      121 gagtatggta gagggaagcg gaattccgca tgtagcggtg aaatgcgtag atatgcggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007055
VERSION     KHUT01007055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           81 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggattgg
       61 aaagtcagtg gtgaaatact ccgacttaat cggagaaccg ccgttgatac tatcaatctt
      121 gaatgtggtt gaggcgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacacccat tgcgaaggca gctcgctaag ccaccattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007056
VERSION     KHUT01007056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c           98 g           52 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcat
       61 tgcgtcgtcg gtgaaattcc acagctcaac tgtggtattg ccgtcgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007057
VERSION     KHUT01007057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           94 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgttc gtgaaatctt gatgcttaac atcaagcgtg cgggcgatac gggcagactt
      121 gagtactaca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg tagtaactga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007058
VERSION     KHUT01007058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           96 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccga
       61 aaagtcaggg gtgaaatcct ccagcccaac tggagaagcg cctttggtac tgtcggcctc
      121 gaggggtgca ggggcatgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007059             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007059
VERSION     KHUT01007059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           85 g           55 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggtgcgta ggcggtttgg
       61 aaagtcagat gtgaaatcct acagcttaac tgtagaactg catttgaaac tcccagacta
      121 gagtatggaa gaggtgaagg gaattcccgg tgtaagggtg aaatctgcag atatcgggaa
      181 gaacaccagt agcgaaggcg cttcactggt ccattactga cgctgagtgc acgaaggtgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007060
VERSION     KHUT01007060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcaggg
       61 tgagttagat gtgaaatgcc aaggcttaac ctcggcactg catttaatac tgccttgctt
      121 gagcgctagc gagggggctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg agcccctaga tagctgctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007061
VERSION     KHUT01007061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcttct
       61 taagtcaggt gtgaaagccc acggctcaac cgtggagggc catctgaaac tggggagctt
      121 gagtgcagga gaggagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacccgt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007062
VERSION     KHUT01007062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           99 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggat
       61 caagtcaacg gtgaaagctc ggcgctcaac gccgagtcgg ccgtcgatac tggctcgctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007063
VERSION     KHUT01007063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgg
       61 caagtctggt gtgaaatctt gcggctcaac cgcaagcggt cactggaaac tgcctcgcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007064             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007064
VERSION     KHUT01007064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          100 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggcttga
       61 gaagtccatg ggtgaaatgc cggagctcaa cttcgtgcgt gcccaggata ctctcaggct
      121 ggagacacgt agaggctggc ggaattccgg gtgtagcggt ggaatgcgta gatatccgga
      181 agaacaccgg aggcgaaggc ggccagctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007065
VERSION     KHUT01007065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           98 g           45 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccgga
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaactg cattcgatac tgtctggctc
      121 gagtccggga ggggatggtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg accatctgga ccggaactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01007066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007066
VERSION     KHUT01007066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggtgtga
       61 taagtggtgt atgaaatctc cgcgcttaac gtggaggggt tatgccagac tgtcacactc
      121 gaacccatca gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaagcg gcgtcctggg gtgaggttga cgctcagaca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007067
VERSION     KHUT01007067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcgggagag
       61 caagtccggt gtgaaagccc ccggcttaac cggggaaggt cgctggaaac tgctcttctt
      121 gaggtggaca gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccat ggcgaaggca gcttcctggg tccaatctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007068             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007068
VERSION     KHUT01007068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcgggttgg
       61 cgcgtccgtc gctgaaatcc cacggctcaa ccgtggaacc ggcgtggata cggccagcct
      121 ggaggggtgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acactttctg acgctgagac gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007069             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007069
VERSION     KHUT01007069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcag
       61 taagttaggt gtgaaagctc cgggctcaac tcggagaggc cacctgatac tgctgtgact
      121 cgaatccagt agaggagtgt ggaatttctg gtgtagcggt gaaatgcgca gatatcagaa
      181 ggaacaccac tagcgaaggc agcactctgg gctggtattg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007070
VERSION     KHUT01007070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 gacgaactgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgtc
       61 caagtcaggg gtgaaatcct gcagcttaac tgcagaagtg cctttgatac tggatgtctg
      121 gagggaggta ggggtgtgtg gaactcacag tggagcggtg aaatgcgttg atattgtgag
      181 gaacgccgga ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007071
VERSION     KHUT01007071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           87 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaagta ggtggtcata
       61 taagtctggg gtgaaatccc tgagctcaac tcaggacgtg ccttggaaac tgtatgacta
      121 gagtatcgga gaggttcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagc ggcgaaagcg gcgaactgga cgataactga cactcaattg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007072
VERSION     KHUT01007072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttttg
       61 taagtcgggt gtgaaatccc caggctcaac ctgggaactg cattcgagac tgcatgactg
      121 gagtatggga gaggacagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctgtctggc ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007073
VERSION     KHUT01007073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggcttcg
       61 cgagtccgat gtgaaagccc agggctcaac cctggaagtg cattggatac tacggagctg
      121 gaggcctgga gaggtgggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctcactgga caggtcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007074
VERSION     KHUT01007074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           98 g           38 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggcctca
       61 caagcgggat gtgaaagctc ctggctcaac tgggagagtg cgttccgaac tgtggagcta
      121 gaggccaggc gatgggggtg gaattcccgg tggagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgcaagcg gcccccaagc ctgagactga cgctgaagcg cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007075
VERSION     KHUT01007075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           90 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggataat
       61 caagctggat gtgaaagacc tgggcttaac ctgggggtgt cattcataac tggttatcta
      121 gagtatggga gaggggagtg gaattcacag tgtagcggtg aaatgcgtag atattgtgag
      181 gaacatcgat ggcgaaggca gctctctggc ctgatactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007076             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007076
VERSION     KHUT01007076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctcg
       61 taagttggat gtgaaagccc taggcttaac caaggaagtg cattcaaaac tgtgaggctt
      121 gagtacagga gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ctgatactga cgctgagggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007077
VERSION     KHUT01007077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ctgggcgtaa agcgcgttaa ggcggccggc
       61 aaagttggtt gtgaaatttc agggctcaac cctgaaactg cgtccaatac tagtcggcta
      121 gagtgcgaga gaggaaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggc tcgcaactga cgctgtagcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007078
VERSION     KHUT01007078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          104 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtga ctgggcgtaa agggcccgca ggcggtcgcc
       61 cgcgttgtga gtgacagctc ccggcttcac tgggagagga ttcagaaaac ggggcgactt
      121 gagggtgaga gagggacacg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc tcatagctga cgctgagggg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007079
VERSION     KHUT01007079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          102 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctaga
       61 tttgtcaggc gtgaaagccc cgggctcaac ctgggaatgg cgcttgggac ggtttggcta
      121 gagatcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007080             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007080
VERSION     KHUT01007080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa aacgctcgta ggtggctacg
       61 tcaagtcgga cgtgaaatct cccggcttaa ctgggagagg tcgctcgata ctggcgagct
      121 cgaggcaggt agggggaagc ggaattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggcttcctgg acctgatctg acactgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01007081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007081
VERSION     KHUT01007081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           84 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgtgg
       61 aaagtcagat gtgaaatccg ggggctcaac ctccgaattg catccgatac taccatgcta
      121 gagaactgga ggggagtctg gaattcttgg tgtagcagtg aaatgcatag atatcaagag
      181 gaacactagt ggcgaaggcg agactctgga cagtatctga cgctcatgca cgaaggtcag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007082
VERSION     KHUT01007082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcacgta ggcggcccgg
       61 taagtccgct ctgaaagtca gaggctcaac ctctggaggc gagtggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctggg acggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007083             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007083
VERSION     KHUT01007083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcggat gtgaaaactc caggctcaac ctggagacgc cattcgatac tgctgtggct
      121 cgagtccggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg accggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007084
VERSION     KHUT01007084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 cacgtcggct gtgaaaacct ggggctcaac cccgggcctg cagccgatac gggcggacta
      121 gaattcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatattga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007085             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007085
VERSION     KHUT01007085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           48 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcaggt gtgaaaactg gaggctcaac ctccagcctg catcagatac tgccatgact
      121 agagtccggt aggggagaat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggttctctgg gccggtactg acgctgagga gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007086             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007086
VERSION     KHUT01007086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           48 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcggat gtgaaaactc agggctcaac ccggagacgc catccgatac tgctgtggct
      121 tgagtctggt aggggaacac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccga tggcgaaggc agtgttctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007087             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007087
VERSION     KHUT01007087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcggat gtgaaatctc cgggctcaac ccggaaactg cacccgatac tgcggtgact
      121 ggagtctggt aggggatcac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccga tggcgaaggc agtgatctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007088
VERSION     KHUT01007088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggttggg
       61 taagtctttt gtgaaagctc ctggcttaac tgggagaggt caaaggaaac tacccggcta
      121 gagtgtggca gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007089
VERSION     KHUT01007089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgagggga gctagcgtta ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtaagag gtgaaagcct ggagctcaac tccagaactg ccttttagac tgcatcgctt
      121 gaatccagga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ctggtattga cgctgaggtg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007090
VERSION     KHUT01007090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccga
       61 taagtcagct gtgaaagtcg ggggctcaac cctcgaaagc cggttgatac tgtcgggcta
      121 gagtccggaa gaggcaagtg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gcttgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007091
VERSION     KHUT01007091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catgcaaaac tgaataacta
      121 gagtgcagta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctatctgga ctgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007092             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007092
VERSION     KHUT01007092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           52 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggcttgt
       61 taagtgtgaa gtgaaatctc cagtgctcaa cacggaaact gcttcgcata ctggcaagct
      121 tgagggatga agaggtaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccca tggcgaaggc agcttactgg tcatctcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007093             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007093
VERSION     KHUT01007093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           99 g           44 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgcgta ggcggttggg
       61 taagtcggac gtgaaagccc ctggctcaac tgggggaggt cgttcgatac tgcccggctt
      121 gagggtgaga gaggagagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actctctggc tcacccctga cgctgatagc gcgaaagcgt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01007094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007094
VERSION     KHUT01007094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacagaggtc gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcttg
       61 cgtgtcagat gtgaaatctc cgggctcaac ccggaaagtg cgtctgaaac tacaggactc
      121 gagtctggga gagggaatcg gaatataggg tgtagcggtg aaatgcgttg atatcctata
      181 gaacaccgga ggcgaaggcg gattccttga acagcactga cgctgaggcg cgaaagtcgg
      241 gggagcaaac agg
//
LOCUS       KHUT01007095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007095
VERSION     KHUT01007095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           62 c          110 g           33 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcggct
       61 taagcgcgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcggac tgagcggctc
      121 gagcactgta gaggcgggtg gaactccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007096             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007096
VERSION     KHUT01007096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           86 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggctgtcaga
       61 taagtcagtt gtgaaattcc ccggcttaac cgggtggact gcgattgata ctgtttgact
      121 tgaatgaggt tgaggtaggc ggaatgtggc atgtagcggt gaaatgctta gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg gccttcattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007097             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007097
VERSION     KHUT01007097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           84 g           62 t
ORIGIN      
        1 caccggcagc tctagtggta gcagttttta ttgggcctaa agcgtccgta gccggtttaa
       61 taagtctctg gtgaaatcct gtagcttaac tgtgggaatt gctggagata ctattagact
      121 tgagaccggg agaggttaga ggtactccca gggtagaggt gaaattctgt aatcctggga
      181 ggaccgcctg ttgcgaaggc gtctaactgg aacggttctg acggtgaggg acgaaagtta
      241 ggggcgcgaa ccgg
//
LOCUS       KHUT01007098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007098
VERSION     KHUT01007098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           36 c           87 g           59 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgta
       61 taagttggat gtgaaagtcc tgggcttaac ctggggattg cgttcaatac tgtatagcta
      121 gagtactgta gaggaaggtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007099
VERSION     KHUT01007099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacccg
       61 taagtcagag gtgaaagcct ggagcttaac tccagaactg cctttgatac tgcgggtctt
      121 gaggatggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007100
VERSION     KHUT01007100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           42 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccttg
       61 tcagtcaggc gtgaaagccc cgggctcaac ctgggaacgg cgcttgggac ggcgaggctg
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007101
VERSION     KHUT01007101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           42 c           97 g           57 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggat gtgaaagccc ggggcttaac tccgggtctg cattcgatac gggcagacta
      121 gagtgtggta ggggagatcg gaatttttgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacattggt ggcgaaggtg gatctctggg tcattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007102
VERSION     KHUT01007102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           97 g           41 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtacctt gtgaaatcct gcggctcaac cgcagaacgg ctgggtatac tggcggtctt
      121 gggcgaccca ggggctgtcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccggc ggtgaaaacg gacagctggg ggtctgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007103             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007103
VERSION     KHUT01007103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgctttaa ggcggttcgc
       61 taagtcgcgt gtgaaacctc caggctcaac ctggagacgc cacgcgatac tggcgtgact
      121 agagttcggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccgacactg acgctgtaga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007104             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007104
VERSION     KHUT01007104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           54 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggttatg
       61 taagtcagat gtgaaaacca tcggctcaac cgattggcat gcatttgata ctgcatgact
      121 tgagttcagt agaggagagc ggaattccca gtgtagcggt ggaatgcgta gatattggga
      181 agaacaccag tggcgaaggc ggctctctgg actgatactg acgctgaggc gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007105             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007105
VERSION     KHUT01007105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           84 g           56 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttactg gtggcaaatc ggggcccaac cccgtggaag ttggtaatac tattagacta
      121 gaggactgga gaggttaacg gaatggttcg tgtaggggta aaatccgcag atatgagttg
      181 gaacaccaaa cggcgaaggc agttaactat tcagtgcctg acgttgaggc acgaaagcgt
      241 gggtagcaag acgg
//
LOCUS       KHUT01007106             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007106
VERSION     KHUT01007106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          101 g           42 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggctcgg
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggagg ccaggggata ctgccgagct
      121 ggagacaagt agaggcgagt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggctcgctgg acttggtctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007107             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007107
VERSION     KHUT01007107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 ttagtcggct gtgaaatcct ggggcttaac cccagaaatg cagtcgatac tgctgtggct
      121 agagtctgga agaggagaac ggaattcccg gtgtagcggt ggaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggttctctgg gccagtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007108
VERSION     KHUT01007108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgct gtgaaatccc gcagcttaac tgcggaactg cattggaaac tatccgacta
      121 gaggaatgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007109             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007109
VERSION     KHUT01007109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           89 g           48 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcgggt gtgaaaactc agggctcaac cctgagctgc cacccgatac tgctgtgact
      121 tgagtctggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccacgactg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007110
VERSION     KHUT01007110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtccggg gtgaaagccc acggcccaac cgtggaagtg ccttggatac tgatggcctc
      121 gagggggata ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007111             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007111
VERSION     KHUT01007111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          102 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacgc
       61 caagtatcga gtgaaatctc cctgctcaac ggggagggct gctcggtaaa ctggcgagct
      121 agaggacggt agaggggggt ggaattgctg gtgtagcggt gaaatgcgta gagatcagca
      181 ggaacacccg tggcgaaggc ggccccctgg gccgaccctg acgctgaggc gcgaaggcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007112
VERSION     KHUT01007112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggttttg
       61 taagatcgat gtgaaatccc cgggcttaac ctgggaactg cattggtgac tgcaaggcta
      121 gagtgtatca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ataacactga cactcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007113             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007113
VERSION     KHUT01007113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           78 g           61 t
ORIGIN      
        1 tacgaaggct ccaagcgtta tccggattta ttgggcgtaa agcgtgcgca ggtggtttga
       61 tgcgtcttgt gttaaatcct attgcctaac aatagggctg catgggatac ggtcaaacta
      121 gaggatatga gaggttgctg gaactcacag tgtaggggtg aaatccgttg atattgtggg
      181 gaacaccaaa ggcgaaggca agcaactggc atatttcctg acactcatgc acgaaagcta
      241 gggtcgcgaa tggg
//
LOCUS       KHUT01007114             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007114
VERSION     KHUT01007114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           85 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggttcga
       61 taagtcagtt gtgaaattcc tcggcttaac cgagtggact gcgattgata ctgtcgaact
      121 tgaatcaggt tgaggtaggc ggaatgtgac atgtagcggt gaaatgctta gatatgtcat
      181 agaacaccga ttgcgaaggc agcttgctgg gcttcgattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007115
VERSION     KHUT01007115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcttgta ggtggccaac
       61 taagtcaaac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggatggctt
      121 gagtttggga gagggacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga ccaatactga cactgaaaag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007116
VERSION     KHUT01007116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           98 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcccac
       61 atagtcaggc gtgaaattcc tgggctcaac ctgggggctg cgcttgatac gtgtgggctt
      121 gaggacagga gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggc ctgtaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007117
VERSION     KHUT01007117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggcgatctt
      121 gagcagctta ggggcagacg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gtctgctggg agcttgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007118
VERSION     KHUT01007118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           95 g           38 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtgccg
       61 cacgtccggt gtgaaagccc agggctcaac cctggaagcg cactggaaac gacggcgctg
      121 gagtcctgaa gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caggaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007119
VERSION     KHUT01007119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtgatg
       61 tatgtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tacatcgcta
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggacactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007120
VERSION     KHUT01007120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           90 g           49 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatta ctgggcataa agcgcgcgta ggtggcattg
       61 taagcggggg atgaaatccc atggctcacc catggaactg tccctcgaac tgcattgctt
      121 gagggcggga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg actctctggt ccgttcctga cactgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007121
VERSION     KHUT01007121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggctatt
       61 taagtcagat gtgaaaactc agggctcaac cttgaggctg catctgaaac tgagtagcta
      121 gagtgtggga gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag agattcggag
      181 gaacaccagt agcgaaggcg gctttctgga ccataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007122
VERSION     KHUT01007122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           86 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgaa
       61 taagttagat gtgaaatccc cgggctcaac ctgggaactg catttaaaac tgtttagcta
      121 gagtagaaca gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg actttctggg ttcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007123
VERSION     KHUT01007123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccttg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcagagctt
      121 gagtgccgga gaggagggtg gaattctcga agtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg accctctgga cggacactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007124
VERSION     KHUT01007124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggcttgg
       61 taagttagag gtgaaagccc ggagctcaac tccggaattg cctttaagac tgcttcgctc
      121 gaatcatgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga catgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007125
VERSION     KHUT01007125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           99 g           39 t
ORIGIN      
        1 aacgtaggac gcgagcgtta tccggagtta ctgggcgtaa agggcgggta ggcggtcagt
       61 caagttggat gtgaaatctc ccggctaaac tgggaggggg cattcgaaac tgattggcta
      121 gaggacagca gagggtcgcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaagcg gcgacctggg ctgtaactga cgctgaaccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007126
VERSION     KHUT01007126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           95 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcaagg gtgaaacctt tcggctcaac cggaagactg cccctgatac tggtcgcctc
      121 gagggaggca ggggcacgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctttcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007127             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007127
VERSION     KHUT01007127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           47 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 taagtcggat gtgaaaactc agggctcaac cctgagacgc cattcgatac tgcggtgact
      121 ggagtccggt agaggagtgt ggaattcctg gtgtagcggt gaaatgcata gatatcagga
      181 ggaacaccaa cggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007128
VERSION     KHUT01007128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatcgt
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggcggtctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007129             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007129
VERSION     KHUT01007129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           96 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 caagtcggat atgaaatctc cacgctcaac gtggaggggt tattcgatac tgcggtgact
      121 agagtccggt aggggagtgt ggaattcctg gtgtagcggt gaaatgcgca gagatcagga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgtgactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01007130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007130
VERSION     KHUT01007130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           85 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ctgggcgtaa agagcgtgca ggtggttacc
       61 taagtcagtt gttaaagacc ccggctcaac tggggaaaag cgattgatac tgagtaactc
      121 gagggcaaga gggaggagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat tgcgaaggca gctccttggc ttgtttctga cactcaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007131
VERSION     KHUT01007131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          100 g           40 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctctt
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaactg cgcctgggac tggcgagcta
      121 gagatcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007132             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007132
VERSION     KHUT01007132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagcacgta ggcggcggcg
       61 taagtggggg gtgaaaggct atcggctcaa ccgtggcatt gccttccaaa ctgcgtcgct
      121 cgaggaaggt aggggcaggt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacctgctgg atcttttctg acgctgaggt gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007133             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007133
VERSION     KHUT01007133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ttgggcttaa agagcacgta ggcggcgacg
       61 caagttggag gtgaaatact atcggctcaa ccgtggaatt gcccccaata ctgcgttgct
      121 cgaggaaggt aggggcgtgt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacacgctgg atcttttctg acgctgaggt gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007134
VERSION     KHUT01007134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccata
       61 aaagtcggat gtgaaagccc tcggctcaac caaggaagtg catctgatac tgtatggctt
      121 gagtaccaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007135
VERSION     KHUT01007135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          102 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcggcc
       61 caagtccgtg gtgaaagctc gccgcttaac ggcgagtcgg ccacggagac tgggtggctc
      121 gagcactgta ggggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gtctgctggg cagttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007136
VERSION     KHUT01007136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacagaggtg tcaaacgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcctta
       61 taagtcatgt gtgaaagctc tgggctcaac ccaggaattg catgtgatac tgtaaggctc
      121 gagttcggta gaggagggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gctctctggt ccgaaactga cgctgaggtg cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01007137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007137
VERSION     KHUT01007137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggccct
       61 taagtccggg gtgaaatccc gaagctcaac ttcggaactg ccctggatac tataggcctt
      121 gaattatgtt gaggtaggcg gaatataaca tgtagcggtg aaatgcatag atatgttata
      181 gaacaccgat tgcgaaggca gcttgctaag catatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007138
VERSION     KHUT01007138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtcaga
       61 caagtttgag gtaacagctc ccggcttcac tgggagagcg tctcgaaaac tgcctgactt
      121 gaggaacaga gagggacatg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca atgtcctggc tgtctcctga cgctcatgtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007139
VERSION     KHUT01007139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcgggtttt
       61 taagtcggag gtgaaatcct ggagcttaac tccagaactg cccccgatac tgggaatctc
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007140
VERSION     KHUT01007140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           98 g           48 t
ORIGIN      
        1 tacggaggcc ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcatct
       61 taagtctggt gtgaaaggcg ggggctcaac ccctggactg cattggatac tgggatgctg
      121 gaggactgga gaggagaatg gaattcacgg tggagcagtg aaatgcgtag atatcgcgag
      181 gaaggccagt ggcgaaggcg attctctgga cagtacctga cgctgaggca cgaaggctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01007141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007141
VERSION     KHUT01007141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggtttcc
       61 taagtcaggt gtgaaatccc agggctcaac cctggaactg catttgatac tgggagacta
      121 gagtgtggaa gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccatctggt ctaacactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007142
VERSION     KHUT01007142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggaataa ctgggcttaa agcgtacgta ggcggacgtg
       61 caggcgtcgt gtgaaagccc tcagctcaac tgaggaattg cacggcgaac cgcacgtctt
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaatgccgaa ggtgaaagca ggccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007143
VERSION     KHUT01007143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggcggt
       61 caagtggtgt atgaaatatc ctggctcaac caggagaggt tatgccagac tgaccgtctt
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcataca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007144
VERSION     KHUT01007144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 taggtcctct gtgaaaactc gaggcttaac ctcgagatgt cggtggaaac catttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007145
VERSION     KHUT01007145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatttg
       61 tcagtcaggg gtgaaatccc ggggctcaac ctcggaactg cccttgatac agcaaatctc
      121 gagctcggaa gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccgtagctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007146
VERSION     KHUT01007146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggacctg
       61 ccagtcaggg gtgaaagccc ccggcccaac cggggaagtg cctttgatac tgcgggtctc
      121 gagcgggata ggggcagctg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga tccctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007147
VERSION     KHUT01007147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 gacgtagggg gcaagcgtta ttcggaatca ttgggcgtaa agcgctcgta ggcgggaaga
       61 taagtccgtg ttgaaagacc tcggctcaac tgagggaccg gctcggatac tgtttttctt
      121 gaggcattca gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcacctggg gatgttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007148
VERSION     KHUT01007148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgtacgta ggcggatcct
       61 taagtcggtg gtgaaatcct gaagctcaac ttcagaactg ccttcgatac tggggacctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007149
VERSION     KHUT01007149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           91 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattga ttgggcgtaa agcgcacgta ggcgggctca
       61 taagtcgaat gtgaaagccc cgggcttaac ctgggaattg catccgaaac tatgggtctt
      121 gaatgcttaa gaggttggtg gaatgtctgg tgtaggagtg aaatccgtag atatcagatg
      181 gaatatcgga ggcgaaggcg gccgactggt gatgcattga cgctgaggtg cgaaagttcg
      241 gggagcaaac agg
//
LOCUS       KHUT01007150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007150
VERSION     KHUT01007150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggattta ctgggcgtaa agagcgcgta ggcggttcga
       61 taagtcggag gtgaaatccc tcggcttaac cgaggacccg cgtccgatac tgtcgaactt
      121 gagtgcagga gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ctgtcactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007151
VERSION     KHUT01007151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           89 g           59 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtttcg
       61 ttagtctggt gtgaaatctc ccggcttaac tgggagggtg cgctagatac tgcgaggctt
      121 gagtgttgga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctttctgga caattactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007152
VERSION     KHUT01007152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           79 g           55 t
ORIGIN      
        1 tacggagagt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcgggataa
       61 taagtcagat gtgaaattcc gaagctcaac ttcggagctg catttgaaac tataattctc
      121 gaggattgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagt ttatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007153
VERSION     KHUT01007153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcttt
       61 taagtcgggt gtgaaagcct cgggcttaac ccgagaagtg cactcgaaac tgagaggctt
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007154
VERSION     KHUT01007154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcagat
       61 caagtccggg gtgaaatcct ccagctcaac tggagaagtg ccttggatac tgatttgctc
      121 gagggaggta ggggtcgagg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ctcgactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007155
VERSION     KHUT01007155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          100 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctatg
       61 taagtcactt gtgaaatccc ggggctcaac tccggggccg caggtgagac tgtgtggcta
      121 gagcatggta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccattgctga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007156
VERSION     KHUT01007156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtttg
       61 taagtccgtg gtgaaatctc cgagcttaac ttggaaactg ccatggatac tatgaatctt
      121 gaatactgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007157
VERSION     KHUT01007157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca gatggggcaa
       61 caagtggtgt atgaaatcgc tcagcttaac tgggcggggt tatgccagac tgttgttcta
      121 gagaacggga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacccctga ggcgaaggcg gcgtcctggc ccgattctga cattcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007158
VERSION     KHUT01007158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggccagta ggcggccgat
       61 taagtccgac gtgaaatccc caggcttaac ctgggaactg cgtcggatac tgatgggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgacattga cgctgaacgg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007159
VERSION     KHUT01007159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           82 g           51 t
ORIGIN      
        1 gacgtaccac ccaaacgtta ttcggattca ctgggcttac agagtccgta ggcggtcgag
       61 aaagttggat gtgaaatacc tcagctcaac tgaggaattg cgttcaaaac tacttggcta
      121 gagagataca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tatcttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007160
VERSION     KHUT01007160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcgggg
       61 taagtctggt gtgaaatctc ggagctcaac tccgaaattg cactggatac tgctctgcta
      121 gagggctgga gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagctcctga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007161
VERSION     KHUT01007161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtggattgg
       61 taagtcagtg gtgaaatact ttcgcttaac gaaggaactg ccattgatac tattaatctt
      121 gaatgtagtg gaggtaggcg gaatgcgtca tgtagcggtg aaatgcttag atatgacgca
      181 gaacaccaat tgcgaaggca gcttgctaca ctactattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007162
VERSION     KHUT01007162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           93 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggcttct
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggatac tgaggggcta
      121 gagtgtggga gaggaaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggtgtaaacg gctttctgga ccattactga cgctgatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007163
VERSION     KHUT01007163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           92 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggcggtttct
       61 taagtctgat gtgaaatctt gcggctcaac cgcaagcggt cattggaaac tggggaactt
      121 gagtgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggtg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007164
VERSION     KHUT01007164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtcaggg gtgaaagccc tgggcttaac ccaggaagtg cccttgaaac ggtgggacta
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatctga ggcgaaggcg gcgacctgga cagtgactga cactcaacta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007165
VERSION     KHUT01007165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ctcggaatca ttgggcgtaa agcgagtgta ggtggctttg
       61 taagtcagga gtgaaagccc taggcttaac ctaggaagtg cttttgatac tgcaaagctt
      121 gaatgtggga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ccaacattga cactgagact cgaaagtgca
      241 gggatcaaac agg
//
LOCUS       KHUT01007166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007166
VERSION     KHUT01007166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           86 g           46 t
ORIGIN      
        1 tacagagact gcgagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccaag
       61 tgtgttaggc gtgaaagcct gaggcttaac ctcagaattg cacctaaaac tacatggcta
      121 gagcattgga gagggtagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01007167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007167
VERSION     KHUT01007167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          100 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggcccgt
       61 taagtgcatg gtgaaagctc ccggctcaac tgggagaggt ctgtgtatac tggcgggctt
      121 gaggggggta gaggaaagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gctttctggg ccctacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007168
VERSION     KHUT01007168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaggcc
       61 taagtacctt gtgaaatccc atggctcaac catggaactg cttggtatac tgggtctctt
      121 gagccaccta ggggcaagtg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca acttgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007169
VERSION     KHUT01007169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcggg
       61 caagtatctt gtgaaatccc acggctcaac cgtggaactg ctggatatac tgcctgtctt
      121 gagccagcca ggggcagtcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaaa ggtgaaaaca gactgctggg gctgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007170
VERSION     KHUT01007170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggtggacggt
       61 taagtggtgt atgaaatgtc ccagctcaac tgggagaggt tatgccagac tggtcgtcta
      121 gggtatggga gagggatgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcatcctggc ccattaccga cactcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007171
VERSION     KHUT01007171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctta
       61 taagtcggat gtgaaatccc tgggcttaac cgaggaagtg cattcgaaac tgcaagactt
      121 gagtacagga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgatctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007172
VERSION     KHUT01007172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatgtt
       61 taagtcagag gtgaaagccc agggctcaac cttggaactg cctttgatac tggacatctt
      121 gagtatggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactggc ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007173
VERSION     KHUT01007173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggttacc
       61 taagttggat gtgaaagccc ttggcttaac cgaggaagtg catccaaaac tgggcagcta
      121 gagtgtggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggt ccgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007174             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007174
VERSION     KHUT01007174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           47 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgact gtgaaaaccc acggctcaac cgtgtggcct gcagtcgata cgggcagact
      121 agaattcggt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgatattg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007175
VERSION     KHUT01007175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           91 g           43 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtcgggt gtgaaatccc ggagctcaac tccggaactg cacccgatac tgcctcgcta
      121 gagtcccgga gaggatggcg gaatttccgg tgtagaggtg aaattcgtag atatcggaag
      181 gaacaccagc ggcgaaggcg gccatctgga cggtgactga cgctcagacg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01007176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007176
VERSION     KHUT01007176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggcttgg
       61 caagttgggt gtgaaagccc agggctcaac cctggaattg cactcaagac tgctttgctt
      121 gaattcggta gaggttggtg gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaacacccgt ggcgaaggcg gccaactgga ccgacattga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007177
VERSION     KHUT01007177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatcag
       61 aaagtagggg gtgaaatccc agggctcaac cctggaattg ccttctaaac tcctggtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007178
VERSION     KHUT01007178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          101 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcct
       61 ttggtcgtgg gtgaaatccc ccagctcaac tggggaagtg cttacgatac cggggggctc
      121 gagggaggaa ggggcggccg gaactgtcgg tggagcggtg aaatgcgttg atatcgacag
      181 gaacgccggt ggcgaaagcg ggccgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007179
VERSION     KHUT01007179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaaccg ctgggcagac tggcggtctt
      121 gagaggccga ggggccatcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccggc ggcgaaagcg gatggctggt ggccttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007180
VERSION     KHUT01007180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaaagg cattgaatac tatctggcta
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007181
VERSION     KHUT01007181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtccgtg gtgaaatact tcggcttaac cggagaagtg ctgcggatac tggtggtctt
      121 gaggggggta ggggcctgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgggctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007182
VERSION     KHUT01007182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtatgt
       61 taagtttgtc gttaaagact ctggcctaac cagagggttg cgatgaaaac tggcaagcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007183
VERSION     KHUT01007183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtatctt gtgaaatccc ccggctcaac cggggaaatg ctggatatac tggcggtctt
      121 gagcgatcta ggggcagatg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaaa ggtgaagaca gtctgctggg gatctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007184
VERSION     KHUT01007184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          110 g           35 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtga ctgggcgtaa agcgcgcgca ggcggcctgt
       61 taggcgcgac gtgaaagccc cgggctcaac ccgggagggt cgtcgcgaac cggcgggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtgctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007185
VERSION     KHUT01007185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           97 g           40 t
ORIGIN      
        1 gacgtagggg gcgagcgtta ttcggaatca ttgggcgtaa agcgctcgta ggcggctccg
       61 caagtccgtg aagaaagacc tgggctcaac ccagggaacg gcacggatac tgcggggatt
      121 gagggaatca gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcacctggg gttcaactga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007186
VERSION     KHUT01007186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggtttct
       61 caagtccgct gtgaaatccc cgggctcaac ctgggaatgg cagtggaaac taggagactt
      121 gaatctggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007187
VERSION     KHUT01007187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           82 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcaagg
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaactg cattgaatac tatctcgctc
      121 gaggattgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga caaatcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007188
VERSION     KHUT01007188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcgcgt
       61 taagtccggg gtgaaagccc gttgctcaac aacggaactg ccccggaaac tggcgcgctt
      121 gagtccagac gagggcggcg gaatggaagc tgtagcggtg aaatgcatag atagcttcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007189
VERSION     KHUT01007189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgc
       61 caagtcagat gtgaaagccc cgggctcaac ctgggaaccg catttgagac tggccgactc
      121 gagttcggga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgag ggcgaaggca gctatctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007190
VERSION     KHUT01007190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttgaa
       61 taagtcgatt gtgaaatccc cgggcttaac ctgggaattg cattcgatac tgttcagcta
      121 gagtgtggta gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaacactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007191
VERSION     KHUT01007191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgca ggcgggagat
       61 taagtcaggg gtgaaagacg gttgctcaac aatcgcagtg cctttgatac tggtcttctt
      121 gggtgcccgt gaggtaggcg gaatgggtgg tgtagcggtg aaatgcatag ataccaccca
      181 gaactccgat tgcgaaggca gcctactggc gggtaaccga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007192
VERSION     KHUT01007192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           96 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcggatcag
       61 caagtcaggg gtgaaagccc ggggctcaac cctggaatgg cccttgatac tgttggtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007193
VERSION     KHUT01007193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           86 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggctgcgcgg
       61 gcagtcaatt gtggaattca agggctcaac ccttgggctg cacttgatac ttccgcgctc
      121 gagccctgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacgccaac ggcgaaagca agactctgga caggtgctga cgctcatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007194
VERSION     KHUT01007194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacagagacg gcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagtcggat gtgaaatccc atggcctaac catggaagtg catccgaaac tccctcgctc
      121 gaggactgga ggggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007195             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007195
VERSION     KHUT01007195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          103 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggccctt
       61 taagtctgag gtgaaaggtt cagcgctcaa cgtgaacagt gccttggata ctggggggct
      121 tgagtgtggc agggggggac ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggtcccctgg gctaacactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007196
VERSION     KHUT01007196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggtgtgta ggcggatact
       61 taagtcgggg gtgaaagccc agggctcaac cctggaatag ccttcgatac tgggtatctt
      121 gagttcggga gaggtaagcg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactggc ccgatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007197
VERSION     KHUT01007197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggtggtctga
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggc caatggatac tgtcggactt
      121 gaatgtggga gaggtgactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaat ggcgaaagca ggtcactgga ccactattga cactcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01007198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007198
VERSION     KHUT01007198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcgggtttg
       61 tatgtcgatt gtgaaagccc cgggctcaac ctgggaattg cagtcgaaac tgcagatctt
      121 gaatacctaa gagggtggga gaattgctgg tggagtagtg aaatacgtag agatcagcag
      181 gaatacctga ggcgaaggcg cctacctggt agtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007199
VERSION     KHUT01007199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggagtta ctgggcgtaa agggcgcgta ggcggcgtct
       61 caagttaggc gtgaaagccc cgggcttaac ctgggaattg cgcttaagac tgagatgctt
      121 gagtgcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007200
VERSION     KHUT01007200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagtt gtgaaatccc cgggcttaac ctgggaactg caacttatac tgtgtaactg
      121 gagtgcaata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ttgacactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007201
VERSION     KHUT01007201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           51 c          103 g           48 t
ORIGIN      
        1 tacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctgtc gtgaaagccc acggcttaac tgtgggtctg cggtggatac gggcaggcta
      121 gaggcaggta ggggagcatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gtgctctggg cctgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007202
VERSION     KHUT01007202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgtgta ggtggattta
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaattg cgttgaagac tgtaagacta
      121 gagttggaca gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacacccgt ggcgaaggcg gctacctggg ttcaaactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007203
VERSION     KHUT01007203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcaggta ggcggttcca
       61 tatgtccttt gtgaaagccc tcggctcaac cgaggaactg caggggaaac tgtgggactt
      121 gagtccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggtactga cgctcagctg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007204
VERSION     KHUT01007204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           83 g           58 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcggatgta ggtggttgca
       61 caagtcgaat gtgaaatccc tcagctcaac tgaggaagtg cattcgaaac tatgtagcta
      121 gaagacagta gagggaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaatacctga ggcgaaggcg gcctcctggg ctgttcttga cactgagatc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007205
VERSION     KHUT01007205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           85 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtctga
       61 taagtctgtt gtgaaagctc ctggcttaac tgggagaggt caacagatac tgtcaggctt
      121 gagtatggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007206
VERSION     KHUT01007206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggtccgta ggtggcccgg
       61 taagtccggc gtgaaatccc agagctcaac tctggaactg cgttggaaac tatcgggctt
      121 gagtgccgga gaggttaggg gaattccagg tgtaagggtg aaatctgtag atatctggag
      181 gaacaccagt ggcgaaggcg cctaactggc cggttactga cactgaggga cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007207
VERSION     KHUT01007207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           86 g           59 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtgtgta ggctgccatt
       61 caagtcagtt gtgaaatccc cgggcttaac ccgggaagtg cttctgatac tgtttggctt
      121 gagtactgga gaggatagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gctatctggc catgtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007208
VERSION     KHUT01007208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgtatgta ggtggttttc
       61 taagtctgat gtgaaatccc agggctcaac cctggaactg cattggatac tggagagcta
      121 gaggatgata gaggatggtg gaattccagg tgtaggggtg aaatccgtag atatctggag
      181 gaacatcagt ggcgaaggcg gccatctgga tcatacctga cactgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007209
VERSION     KHUT01007209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          100 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggctctt
       61 gaagtcaggc gtgaaagccc cgggctcaac ctgggaggtg cgcttgatac tcgagggctg
      121 gagtacggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007210             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007210
VERSION     KHUT01007210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           46 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcaggt gtgaaatctc cgcgctcaac gcggaggagc cacctgatac tgccgtgact
      121 tgagtccggt aggggagtgc ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccaa tggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007211
VERSION     KHUT01007211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           92 g           44 t
ORIGIN      
        1 aacgtagggg gctagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggttctt
       61 taagtcggat gtgaaatcca gcggcccaac cgctgaattg catccgatac tggggaactt
      121 gaggacggta gaggtcggtg gaattcccgg tgtaccggtg aaatggacag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01007212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007212
VERSION     KHUT01007212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccagattta ctgggcgtaa agcgcgcgca ggcggattgt
       61 taagtcagtc gtgaaagccc ccggctcaac tggggagggt cgtctgaaac tggcagtctt
      121 gaggacaaca gaggagtgtg gaattgcggg tgtagtggta aaatgcgtag atatccgcag
      181 gaacaccagt ggcgaaagcg gcactctggg ttgcacctga cgctgaggcg cgaaggctag
      241 gggagcgaac agg
//
LOCUS       KHUT01007213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007213
VERSION     KHUT01007213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctggt gttaaataca ggagctcaac tcctgcatgg cactggaaac tgggcggcta
      121 gagtatggta gaggtcacgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg cgtgactggg ccataactga cgctgaggaa cgaaagctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01007214             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007214
VERSION     KHUT01007214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           68 c           98 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgtc ggcggcccgg
       61 cacgtccgtc gttgaaagcc cccggctcaa ccggggaatc ggcgcggata cgaccgggct
      121 ggagggtggt agggggagcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggctcctgg accacacctg acgctcagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007215             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007215
VERSION     KHUT01007215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgggtcgtgg gtgaaatccc ccagcttaac tggggaagtg cttacgatac cactgcggct
      121 ggaggaaggc aggggcactt ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgccgg tggcgaaagc ggagtgctgg tccttttctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007216             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007216
VERSION     KHUT01007216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           66 c           96 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtcgatg gttgaaatcc cccagctcaa ctggggaaca ggcctcgata cgaccggcct
      121 cgaggggggt agggggagcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggctcctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007217
VERSION     KHUT01007217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggttac
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cccctgatac tggtagtctt
      121 gagggaggta ggggcatgtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007218
VERSION     KHUT01007218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           75 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggatata
       61 caagtcagag gtgaaagcta acagcttaac tgttaaattg cctttgaaac tgtatatctt
      121 gagtatagtt gaggtgggcg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaactccgat tgcgaaggca gctcactaag ctattactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007219
VERSION     KHUT01007219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agggcgtgca ggtggtctgt
       61 taagtggtgc gtgaaagcgc tcggctcaac cgggcgaggc cgtgccaaac tgggagacta
      121 gaaacaggca gaggcaagtg gaattcgggg tgtagtggtg aaatgcgtag agatcccgag
      181 gaactcctgt ggcgaaggcg acttgctggg cctgctttga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007220
VERSION     KHUT01007220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggtggtctgt
       61 taagtcgatt gtgaaatccc atggctcaac catggaagtg ctgtcgaaac tggcggacta
      121 gagtcccgac gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg agggtactga cactcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007221
VERSION     KHUT01007221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           81 g           57 t
ORIGIN      
        1 tacgtagggg gcaaacgttg tccggattta ctgggtgtaa agggtgctca ggtggttctg
       61 taagtcagag gtgaaatttc ggggctcaac cccggacctg cctttgatac tgcagagctt
      121 gtgtacggaa gaggttgatg gaatttttgg tgtagcagtg aaatgcgtag atatcaaaaa
      181 gaacaccagt ggcgaaggcg gtcaactggt ccggtacaga cactaaagca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007222
VERSION     KHUT01007222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           84 g           54 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcaggg gtgaaatccc acagcttaac tgtggaactg cctttgaaac tgtaaagctg
      121 gaatgcaaga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga ttgcaattga cgctgaggcg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01007223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007223
VERSION     KHUT01007223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           79 g           60 t
ORIGIN      
        1 tacgaaggtc ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggttat
       61 taagtcagtg gtgaaagctc tcggctcaac cgggaaattg ccattgatac tgatgatctt
      121 gagtatgatt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007224
VERSION     KHUT01007224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           92 g           43 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcttgg
       61 taggtcacgt gttaaagcct gcggctcaac cgcagaatcg cacgtgaaac cacctggcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007225
VERSION     KHUT01007225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggactta ctgggcgtaa agcgtgtgca ggcgggccgc
       61 tatgtgccgt gtgaaagctc tcagcttatc tgggagaggt cgcggtagac aggcagcctg
      121 gaggctcgga gaggtcagtg gaattctcgg tgtagtggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgcaagcg gctgactggc cgagacctga cgctgagaca cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01007226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007226
VERSION     KHUT01007226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtcgg
       61 caagtcaggc gtgaaagccc tgggctcaac ccaggaactg cgtttgaaac tgccgatctt
      121 gagtagtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cactaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007227
VERSION     KHUT01007227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtttgt
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg catacgaaac tggcgaactt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcggcctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007228
VERSION     KHUT01007228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           91 g           49 t
ORIGIN      
        1 tacaggggtc tcgagcgtta atcggaatta ctgggcttaa agggtgcgta ggccggcccg
       61 caagtgtctt gtgaaatccc ccggctcaac cggggaactg ctaggcatac tgcgggtctt
      121 gaggaaggta ggggttgcca gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaatgccgat ggtgaagacg ggcaactggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007229
VERSION     KHUT01007229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           82 g           57 t
ORIGIN      
        1 tacgagtgcc tcaagcgtta tccggaatca ttgggcgtaa aggatgtgta ggtggtcata
       61 ttagtctctc gttaaattct tcggcttaac cgggggcatg cgggggaaac ggtatgacta
      121 gaggatgcaa ggggtctacg gaactcatag tgtagcggtg aaatgcgttg atattatggg
      181 gaacaccaaa agcgaaggca gtagactagg gcactcctga cactgaaaca tgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01007230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007230
VERSION     KHUT01007230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggcgatt
       61 caagtctggg gtgaaagcct tcggcttaac cggagaagtg ccttggatac tggatcgctc
      121 gagggggata ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcatgctggg tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007231
VERSION     KHUT01007231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 tacggggggg gctagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgac
       61 tcagtcagac gtgaaatccc tcggctcaac cggggaacgg cgtctgatac tagttggctc
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007232
VERSION     KHUT01007232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggta ggcggtccgg
       61 caagtctgtt gtgaaagctc ccggctcaac tgggagaggt caatggatac taccggactt
      121 gaatgtggga gaggtcactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccgat ggcgaaagca ggtgactgga ccactattga cgctgagcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007233
VERSION     KHUT01007233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           94 g           51 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggcttgta ggcggtttgt
       61 tgcgtcggaa gtgaaaactc agggcttaac cctgagcctg cttccgatac gggcaaacta
      121 gagggatgca ggggagaatg gaattcctgg tggagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg catttcctga cgctgagaag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007234
VERSION     KHUT01007234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacatggggt gcaagcgtta ctcggaatca ctgggcgtaa agagcgcgta ggcggcttgg
       61 taagtctgat gtgaaagccc cggactcaat tcgggaagtg cattggatac tgctaagcta
      121 gagtacagga gaggtgaacg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg gttcactgga ctgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007235
VERSION     KHUT01007235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggtctg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tataggtctt
      121 gaattttctg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctgactaca ggaatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007236
VERSION     KHUT01007236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           97 g           45 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccacg
       61 taagtcggat gtgaaagccc agggctcaac cttggaagtg catccgatac tgtgtggcta
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accgcctgga cgaagattga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007237
VERSION     KHUT01007237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          103 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctggg
       61 taagcgtgcg gtgaaagctc ggagctcaac tccgagtcgg ccgtgcggac tgcctagctc
      121 gagcacggta gaggccgatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtcggctggg ccgttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007238
VERSION     KHUT01007238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           82 g           59 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggtatat
       61 taagtctgtt gttaaatctt ctggcctaac cagaaatctg cagtagatac tgatgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007239
VERSION     KHUT01007239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           92 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggttctc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cattgggaac tggagggctc
      121 gagtatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctttctgga ccatcactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007240
VERSION     KHUT01007240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           79 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggaaaa
       61 taagtcagtg gtgaaagcac cgggctcaac tcggtaactg ccattgatac tgtttttctt
      121 gaatgcgatt gaggtaagcg gaatgttgca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccaat tgtgaagaca gcttactaag tcgtgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007241
VERSION     KHUT01007241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           87 g           54 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ctgggcgtaa agagtgcgta gacggtgtgt
       61 taagtttgtt gttaaagact ctagcctaac tggagatctg caatgaaaac tggcatgctg
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007242
VERSION     KHUT01007242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           42 t
ORIGIN      
        1 tacggggggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggccccg
       61 cgtgtcctct gtgaaagccc tcggctcaac cgaggaactg caggggaaac tgtggggctc
      121 gagtccggga gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccggtactga cgctaaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007243
VERSION     KHUT01007243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           83 g           57 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtatgg
       61 tatgtctgat attaaagact ggagcttaac ttcaggaatg tatcggaaac tgccaaacta
      121 gaggacaata gaggaagtgg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg cacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007244
VERSION     KHUT01007244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtag agggcgcgta ggctgtttgg
       61 taagtagaag gtgaaagact tgggctcaac ccaagaattg ccttctaaac tgccttactt
      121 gaggatctga ggggaaactg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaagcg ggtttctggc agattcctga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007245
VERSION     KHUT01007245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtctggt gtgaaaactt ggggctcaac cctgagatgt catcggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccat ggcgaaggca gctctctggg acggtactga cgctaagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007246
VERSION     KHUT01007246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          103 g           38 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcgc
       61 gcagtcgggt gtgaaagccc ggggctcaac cccggaacgg cacccgagac gcgcgggctc
      121 gagaccggga gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007247
VERSION     KHUT01007247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggcctat
       61 taagtcagat gtgaaatccc ttggctcaac cgaggaactg catctgatac tggtaggctt
      121 gagtgtcgga ggggatggta gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaatacctgt ggcgaaggcg accatctgga agacaactga cgctgagact cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007248
VERSION     KHUT01007248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           82 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggataat
       61 taagtcaaat gtgaaagccc tgggctcaac ctaggaattg catctgatac tggttgtcta
      121 gagtatggta gaggatggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccaat ggcgaaggca gccatctggg ccaatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007249
VERSION     KHUT01007249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcatgta ggtggattat
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tgatagtcta
      121 gagtttaaca gagggctgcg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcagcctggg ttgaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007250
VERSION     KHUT01007250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggcggtgcag
       61 caagtcaggg gtgcaagctc tgggcctaac ccagaaactg cctctgaaac tactgtgctt
      121 gagggtcaaa ggggaagctg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaggcg ggcttctggt tgacatctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007251
VERSION     KHUT01007251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggattta ctgggcgtaa agcgcgagta ggcggtcttg
       61 taagtcgggt gtgaaaaccc agggctcaac cctggcagtg cacacgatac tgcgagactg
      121 gggtactgaa gaggagagcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagataccga cgctgagacg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01007252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007252
VERSION     KHUT01007252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtctat
       61 caagtcagag gtgaaagccc tgggctcaac ccaggaattg cctttgaaac tgatagactt
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007253
VERSION     KHUT01007253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agggcgcgta ggctggcgaa
       61 taagtcgggt gtgaaattct gaggcttaac ctcagagctg cacttgaaac tgtttgtcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagttag
      241 gggatcaaac agg
//
LOCUS       KHUT01007254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007254
VERSION     KHUT01007254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggctatc
       61 taagtcgggt gtgaaatccc tgggctcaac cgaggaagtg catccgaaac tagatggctg
      121 gaagatggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ccattcttga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007255
VERSION     KHUT01007255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           87 g           54 t
ORIGIN      
        1 gacgagctct ccaaacgtta ttcggtatta ctgggcttaa agagttcgta ggcggcttta
       61 caggtgaggt gtgaaagccc tcggctcaac cgaggaattg cgcttcaaac cgtagggctt
      121 gagggagaca ggggtaagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007256
VERSION     KHUT01007256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           91 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcttta
       61 taagtctggt gtgaaatccc ggggcttaac cccggaactg cactggatac tgtaaagcta
      121 gagggcgaga gaggacgatg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gtcgtctggc tcgcacctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007257
VERSION     KHUT01007257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           83 g           55 t
ORIGIN      
        1 tacgaagggg gcaagcgtta ttcggaatta ctgggcgtaa agagcgtgta ggtggttatc
       61 taagtcagtt gttaaatatc acggcctaac tgtgagactg cgattgatac tggatgacta
      121 gagggcagga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ctgtccctga cactcaaatg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007258
VERSION     KHUT01007258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           97 g           41 t
ORIGIN      
        1 tacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agagcgtgta ggcggttgtc
       61 caagtcaccc gtgaaagctc ccggctaaac tgggagaggt cgggtgagac tgggcggcta
      121 gagggttgta gagggatgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcatcctgga caacacctga cgctgagacg cgaaagcgcg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007259
VERSION     KHUT01007259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           86 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcttgg
       61 taagtcaggg gtgaaagctt cgcgcttaac gcgagaactg cctttgatac tgccgagctt
      121 gaaagaggtt gaggttagcg gaacgtgaca tgtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gctgactgga cctactttga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007260
VERSION     KHUT01007260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtctgt
       61 caagcgtgcg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tggtggacta
      121 gagcactgta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007261
VERSION     KHUT01007261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           89 g           56 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgggg
       61 taagttggat gtgaaatccc agggctcaac cctggaactg cattcaatac tgctttgctt
      121 gagtactgga gaggagattg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007262
VERSION     KHUT01007262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttag
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaactg cattcgatac tgctgagcta
      121 gagtatggaa gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg acttcctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007263
VERSION     KHUT01007263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgtgta ggcggttggg
       61 taagtttgtt gtgaaatctc caagctcaac ttggaaaatg caatgaaaac tgcccggcta
      121 gaggattgta gggggaactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agttcctgga caaatcctga cgctgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01007264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007264
VERSION     KHUT01007264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           74 g           67 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcaat
       61 taagtcaggg gtgaaagttt gcggctcaac cgtaaaattg cctttgatac tggttgtctt
      121 gaattattat gaagtggtta gaatgagtag tgtagcggtg aaatgcttag atattactca
      181 gaataccaat tgcgaaggca gatcactaat aatatattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007265
VERSION     KHUT01007265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           74 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcggatttt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tattagtctt
      121 gaattctgtt gaggtaggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gcttactaaa cagatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007266
VERSION     KHUT01007266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtttg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tgcgaatctt
      121 gagctgtcta ggggttgccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggcaactggg gacatgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007267
VERSION     KHUT01007267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggttcac
       61 taagtcgtgc gtgaaagcgc cctgctcaac agggcgagtg cgggcgagac tggtggacta
      121 gagctagata gaggcaagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgaaggcg gcttgctggg tctaagctga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007268
VERSION     KHUT01007268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           92 g           48 t
ORIGIN      
        1 tacatagggt ccgagcgttg tccggaatca ttgggcgtaa agcgcatgta ggcggccctt
       61 taagtcggat gtgaaatccc gaggctcaac ttcggaactg catccgatac tggggggctt
      121 gaggaaggta gaggagagtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg cctctcctga cgctgagatg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007269
VERSION     KHUT01007269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          106 g           34 t
ORIGIN      
        1 gacggaggag gcgagcgtta tccggattta ctgggcgtca agggcgcgta ggcggcctga
       61 caggtcgtgg gtgaaagccc cgggctcaac ccggggaggt cccgcgatac catcaggctt
      121 gaggatcgta gaggggggcg gaatgtccgg tggagcggtg gaatgcgtag atatcggacg
      181 gaacacccga ggcgaaggcg gccccctggg cgatacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaag cgg
//
LOCUS       KHUT01007270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007270
VERSION     KHUT01007270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           83 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagtatgta ggcggatact
       61 taagtagaaa gtgaaaggtt ggagctcaac tccaacattg cttcctatac tgggtatctt
      121 gagtgctgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctttctgga cagagactga cgctgagata cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007271
VERSION     KHUT01007271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           90 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttag
       61 taagttatct gtaaaattcc tgggcttaac ctagggaggt cagataagac tgttagactt
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007272
VERSION     KHUT01007272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcgggtcag
       61 caagtcagat gtgaaatctc gaagctcaac ttcgaaactg cgtctgaaac tgctagtcta
      121 gagtgtcgga gggggaaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggg ggcgaaggcg ccttcctgga cgactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007273
VERSION     KHUT01007273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 tacgagaggc ccgagcgtta ggcggaatca ctgggcttaa agggtgcgta ggcggaccag
       61 taagcatttt gtgaaatccc tcggctcaac cgaggaattg cagagtgaac tactggtctt
      121 gaggtaggca gaggtcatcg gaacgtacgg tggagcggtg aaatgcgtag atatcgtacg
      181 gaacgccgaa ggggtagcca ggtgactggg ccttacctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007274
VERSION     KHUT01007274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           93 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatca ttgggcgtaa agcgcatgta ggcggccatg
       61 taagtcggat gtgaaatccc gaggcttaac ttcggaactg catccggtac tgcgtggctt
      121 gaggaaggta gaggagagtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg cctttcctga cgctgagatg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007275
VERSION     KHUT01007275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           98 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcgact gtgaaatccc gcggctcaac cgcgggcttg cagtcgatac gggcaggcta
      121 gagtttggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccaatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007276
VERSION     KHUT01007276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           84 g           53 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggccctg
       61 taagtcagcg gtgaaatctc ccggctcaac cgggaaactg ccgttgatac tgcagggctt
      121 gaattgcagg gaagtggcta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccgat tgcgaaggca ggccactacc tgcggattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007277
VERSION     KHUT01007277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           83 g           56 t
ORIGIN      
        1 tacgagaggt gcgagcgtta ttcggattta ttgggcgtaa agggtgtgta gacggcgtat
       61 taagtcaact gttaaatcct ccggcctaac cggagtcctg cagtagatac tgatatgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtgg atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007278
VERSION     KHUT01007278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           87 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcgcgta ggcggctcat
       61 caagtgcaga gtgaaatccc acggcttacc cgtggaactg ctctgtatac tgacgagcta
      121 gaggacggta ggggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccgat ggcgaaggca gctctctggt ccgtttctga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007279
VERSION     KHUT01007279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 gacgaactgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcaaa
       61 caagtcaagg gtgaaatcct gcagcttaac tgtagaagtg cctttgatac tgtttgtctg
      121 gagggagata ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007280
VERSION     KHUT01007280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggacatt
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaattg cctttgatac tgggtgtctt
      121 gagtatgaga gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggc tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007281
VERSION     KHUT01007281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           87 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcctgg
       61 taagtcagag gtgaaaggtg ccggctcaac cggcgaattg cctttgatac tgtcgggctt
      121 gaattgagtt gaggttagcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccaat tgcgaaggca gctagctaga cttgtattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007282
VERSION     KHUT01007282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccctg
       61 taagtcaaag gtgaaagccc tcggctcaac cggggaaatg cccttgatac tgcaaggctc
      121 gaggccggga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacccgt ggcgaaggcg gccacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007283
VERSION     KHUT01007283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgcacgca ggcggcgtgc
       61 caagtcgaat gtgaaatccc tgggctcaac ccaggaactg cattcgaaac tggcatgctt
      121 gagtccggga gaggatggtg gaattcctgg tgtagaggtg aaattcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg gccatctggc ccggtactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007284
VERSION     KHUT01007284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           93 g           47 t
ORIGIN      
        1 tacgtaggtc gcgaacgtta ttcggattta ctgggcttaa agggcgtgta ggcggcttcg
       61 caagtcaagg ttgaaatccg gcggctcacc cgtcgaactg gccttgatac tgcggagcta
      121 gagtcatgga ggggtgcgcg gaacttgtgg tggagcggtg aaatgctcag atatcacagg
      181 gaacaccgga ggcgaaggcg gcgcactggc catgtactga cgctgtagcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007285
VERSION     KHUT01007285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcatgg
       61 catgtcgggt gtgaaatccc gcggctcaac cgtggaagtg cattcgaaac tgccaagcta
      121 gagtcctgga gaggaagatg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg gtcttctgga cagtgactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007286
VERSION     KHUT01007286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           93 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatca ttgggcgtaa agcgtacgca ggcggttcgt
       61 caagtcgggt gtgaaagccc ggggctcaac cccggaatag cgcccgaaac tagcgagctt
      121 gagtgcggga gagggaagcg gaattctggg tgtagaggtg aaattcgttg atatccagag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007287
VERSION     KHUT01007287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           75 g           60 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggattta ttgggtttaa agggtccgta ggcggaataa
       61 taagtcagtg gtgaaatcct acagctcaac tgtagaactg ccattgatac tgttgttctt
      121 gagtacactt gaagtaggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat agcgaaggca gcttactaaa ttgtaactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007288
VERSION     KHUT01007288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gctagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cactcgatac tgatcgactt
      121 gaagatggga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggc ccattcttga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007289
VERSION     KHUT01007289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           87 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgttt atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagtcagtt gtgaaatccc cgggcttaac ctgggaattg catctgatac tgtcgaacta
      121 gagtgtgata gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcttactgga ttaacactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007290             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007290
VERSION     KHUT01007290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           44 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagttgggt gttaaacccc caggcttaac ctggggacgc cacccgatac tgccgtggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007291             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007291
VERSION     KHUT01007291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcttgg
       61 caagtttggc gtgaaatctc cgggcttaac ccggaaatgg cgttgaatac tgtttgagct
      121 ggaggtttgg aggggggact ggaattcttg gtgtagcagt gaaatgcgta gatatcaaga
      181 ggaacaccag tggcgaaggc gagtccctgg acaacacctg acactgaggc acgaaagctg
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007292             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007292
VERSION     KHUT01007292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c          100 g           38 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcccg tgtgaaatcc cccggctcaa ccggggaacg gccgggcaga ctggcggtct
      121 tgaggcaccc aggggctgtc ggaacaagcg gtggagcggt gaaatgcgta gatatcgctt
      181 ggaacgccgg cggtgaaaac ggacagctgg gggtgttctg acgctgagac acgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007293             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007293
VERSION     KHUT01007293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           52 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtga
       61 caagtcattt gtgaaatctc cgggcttaac tcggaacggt caagtgatac tgtcgtgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ctgacactga cgctgacagc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007294             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007294
VERSION     KHUT01007294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccttc
       61 caagtcaggg gtgaaatccc agagctcaac tctggaattg cctctgagac tgtgaggctt
      121 cgagtacggg agaggtgagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggctcactgg cccgttactg acgctcaggc gcgacagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01007295             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007295
VERSION     KHUT01007295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           54 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgaa gtgaaatctc cagtgctcaa cacggaaact gctttacata ctggcgagct
      121 tgaggaaagc agagataact ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc aggttattgg gctttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007296
VERSION     KHUT01007296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           42 c           80 g           53 t
ORIGIN      
        1 tacggagagt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggaatga
       61 taagtcagat gtgaaatacc gaagctcaac ttcggtgctg catttgaaac tatcaatcta
      121 gagggttgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaagcg gttttctagt ttatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007297             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007297
VERSION     KHUT01007297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           82 g           53 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ctgggcgtaa agagtgcgta ggtggttcaa
       61 taagttactg gtggcaaatc ggggcccaac cccgtggaag ttggtaatac tgttgaacta
      121 gaggaccgga gaggttaacg gaattctatg tgtagcggta aaatgctcaa atatatagag
      181 gaacaccaaa cggcgaaggc agttaactat tcggtgcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01007298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007298
VERSION     KHUT01007298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccgtt
       61 taagtagggt gtgaaagcct ggagctcaac tccagaactg cgctctaaac tggacggctc
      121 gagtgagaca ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tcttaactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007299
VERSION     KHUT01007299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcttga
       61 aaagttggat gtgaaatccc ggggctcaac cccggaactg cattcaaaac tttctggcta
      121 gagacttaga gaggtgggcg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccggt tgcgaaggcg gctcactggc taagttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007300
VERSION     KHUT01007300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           83 g           53 t
ORIGIN      
        1 tacgtagggc ccgagcatta tccggaatta ttgggcgtaa agaatgtgta ggtgttccta
       61 caagtcggtc gttaaatccc accgcctaac ggtggggccg cgagcgaaac tgtaggaatt
      121 gaggtttgca ggggcaagca gaatttttgg agtaggggtg aaatccgttg ataccaaaag
      181 gaataccaat ggcgaaggca gtttgctggg caatacctga cactgagaca tgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01007301             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007301
VERSION     KHUT01007301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agggctagta ggcggcttgg
       61 caagtcgagc gtgaaaactc caggctcaac ctggagctgc cgtttgatac tgccatggct
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggaactg acgctgaata gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01007302             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007302
VERSION     KHUT01007302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccagc
       61 acaagtcagt tgtgaaatct ctgggcttaa cccagaacgg tcaactgata ctgtgcggct
      121 agagtgcgga aggggcaact ggaattctcg gtgtagcggt gaaatgcgta gatatcgaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgatgc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007303             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007303
VERSION     KHUT01007303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           86 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggcgcgta ggtggcttgg
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaaatg cattgaatac tatttgagct
      121 tgaggattgg aggggggact ggaattcttg gtgtagcagt gaaatgcgta gatatcaaga
      181 ggaacaccag tggcgaaggc gagtccctgg acaacacctg acactgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007304
VERSION     KHUT01007304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg tccggaatca ctgggcgtaa agagcgtgta ggcggatgtg
       61 caagtccggg gtgaaatgtc agggctcaac tctgaacctg ccttggaaac tgcatatctt
      121 gagtgcggta gggggaatcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gattcctggc ccgtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007305             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007305
VERSION     KHUT01007305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcttt
       61 caagttgggg gtgaaatccc agagctcaac tctggaattg ccttcaagac tgtgagactt
      121 cgagtacggg agaggtgagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggctcactgg cccgttactg acgctcaggc gcgacagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01007306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007306
VERSION     KHUT01007306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           75 g           61 t
ORIGIN      
        1 tacagaggcc tcaagcgtta tccggattta ttgggcgtaa agagcatgta ggcggatata
       61 ttagtcagat gtcaaatctt cgagctcaac tcgaaaactg catttgaaac ggtatatcta
      121 gagacagtca gagatcaatg gaacgtatgg tgtagcagtg aaatgcgttg atatcatacg
      181 gaacaccaaa ggcgaaggca tttggttggg gctgttctga cgctgagatg cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01007307             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007307
VERSION     KHUT01007307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgtta ctcggattca ctgggcgtaa agcgcgcgta ggcggctggt
       61 caagcgtggt gtgaaatctc cgggctcaac ccggaaactg catcgcgaac tggcctggct
      121 ggagtgcagg agagggaagg ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc gccttcctgg cctgtaactg acgctgatgt gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01007308             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007308
VERSION     KHUT01007308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           99 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcgatc
       61 atttgtcgag tgtgaaagcc ccgggcttaa cctgggaact gcgctcgaaa cgggatggct
      121 agagatcggg agaggagcgt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggcgctctgg accgatactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007309
VERSION     KHUT01007309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctgc
       61 gaagagagat gtgaaatccc cgggcttaac ctgggaactg cgtttctaac tggcaggctt
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007310
VERSION     KHUT01007310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           82 g           50 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggcttctttc
       61 cacgtctgat gtaaaagcac gaagctcaac tccgtgtacg tgtcggatac gagaaagatt
      121 gagtcattca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgccaaa agcgaaggca ggatgctggg aatgtactga cgctcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01007311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007311
VERSION     KHUT01007311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcttcg
       61 caagtcagac gtgaaagccc cgggcttaac ctgggaatgg cgtttgaaac tacggagctc
      121 gagtatggca gaggggggtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007312
VERSION     KHUT01007312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           86 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggattta ttgggcgtaa agcgcgtgta ggtggttcgg
       61 taagtctgat gtgaaatccc tggactcaat tcaggaagtg cattggatac tgccaaacta
      121 gagtgcagga ggggaaggcg gaattccagg agtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gccttctgga ctgcaactga cactaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007313
VERSION     KHUT01007313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgtct gtgaaaacca gcagctcaac tgttggcttg caggcgatac gggcagactt
      121 gagtatttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aaataactga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007314
VERSION     KHUT01007314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacggagagg gcaagcgttg ttcggattta ctgggcataa agcgcacgta ggcggattca
       61 caagtcggtt gtgaaatccc ggggctcaac cccggaacag cggccgatac tatgagtcta
      121 gagcacgaaa ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggt tcgttgctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007315
VERSION     KHUT01007315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           91 g           44 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcagtg
       61 taagtctgct gtcaaaggtc agggctcaac cctgaacagg cagtggaaac tacacagcta
      121 gagtgcggtg ggggcagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgtaactga cactcatgga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01007316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007316
VERSION     KHUT01007316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           39 c           90 g           59 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtttgat gttaaagatc aaggctcaac cttgggagtg cattgaatac tggcagactt
      121 gagtgcagta gaggcgagtg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaggcg actcgctggg ctgtaactga cgctgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01007317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007317
VERSION     KHUT01007317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           91 g           50 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggttctg
       61 taagtaggat gtgtaatccc cgagctcaac ttgggaactg catcctagac tgcaagacta
      121 gagtgctgga gagggtggta gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccagt ggcgaaggcg gccacctgga cagtaactga cgctgaggcg cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007318
VERSION     KHUT01007318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           87 g           56 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggctagg
       61 taagtcagat gtgaaatccc ggagctcaac tctggaactg catttgatac tgcttggcta
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007319
VERSION     KHUT01007319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcggctgag
       61 tcagtccgac tttaaagacc gaagctcaac ttcgggcctg ggttggatac tgctgagcta
      121 gacggatgga gaggtcactg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccaac ggcgaaggca ggtgactgga cattttgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01007320             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007320
VERSION     KHUT01007320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           82 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agtgtgcgta ggtggtttaa
       61 taagttactg gtggcaaatc gaggctcaac ctcgtggaag tcggtaatac tattagacta
      121 gagggtcgga gaggttaacg gaatggttcg tgtaggggta aaatcctcaa atatgaattg
      181 gaacaccaaa cggcgaaggc agttaactat tcggtacctg acgttgaggc acgaaagtgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01007321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007321
VERSION     KHUT01007321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           94 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccaac
       61 taagtcaatc gtgaaatccc tcggcttaac cggggaactg cgcttgatac tggatggctt
      121 gagtttggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaatactga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007322
VERSION     KHUT01007322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           48 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgca ggcggctatc
       61 taagtcagag gtgaaatgcc tgggctcaac ccaggaactg cctttgaaac tggatggcta
      121 gagtctcgga gggggtggcg gaattcctaa tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gccacctgga cgagtactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007323
VERSION     KHUT01007323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           86 g           57 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agggtttgta ggctggaaga
       61 taagtcaagt gtgaaatccc cgagctcaac ttgggacgtg catttgaaac tgtttttctt
      121 gagtattgga gagggtggtg gaattgttgg tgtaggagtg acatccgtag agatcagcag
      181 gaacatcaga ggcgaaggcg accacctggc caattactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007324
VERSION     KHUT01007324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           87 g           53 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgctg
       61 taagtcgggt gtgaaatccc tcagcttaac tgaggaattg cgcccgatac tgtagtgctt
      121 gagggagata ggggtcagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctgactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007325
VERSION     KHUT01007325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           88 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcctg
       61 taagtctggt ttgaaagcta gctgctcaac agttagatgt ggctggaaac tgtgggactt
      121 gaatgagttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01007326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007326
VERSION     KHUT01007326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           91 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggttttt
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tggaagactt
      121 gagtttagga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctagc ctttgactga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007327
VERSION     KHUT01007327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           81 g           55 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggtatgt
       61 caagtcaact gtttaattct ccggcctaac cggaggtcag cggtagaaac taatatgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccaatcctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007328
VERSION     KHUT01007328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           76 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggattct
       61 taagtcagtg gtgaaagcct acagcttaac tgtagaactg ccattgatac tgagaatctt
      121 gagtacagtt gaggcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007329
VERSION     KHUT01007329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           87 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggctagg
       61 taagtcagat gtgaaaaccc agggctcaac cctgggactg catttgaaac tatctagcta
      121 gagtgtcgga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cgataactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007330
VERSION     KHUT01007330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           94 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggttttc
       61 taagtctgat gtgaaatccc ggggcttaac tccggaactg cattggatac tggaaagcta
      121 gaggacgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcagggg
      181 gaacatcggt ggcgaaggcg gccatctggc tcgtgcctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007331
VERSION     KHUT01007331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagtgta ggcgggttga
       61 taagtcagtg gtgaaagccc taagcttaac ttgggaattg ccattgatac tgtcagtctt
      121 gagtatggtt gaggtaggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gcttaccaaa ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007332
VERSION     KHUT01007332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           95 g           44 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtttag
       61 taagtcgaat gtgaaatccc agggctcaac cgtggaagtg catccgaaac tgctagacta
      121 gagtcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007333
VERSION     KHUT01007333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgctggta ggtggcttgt
       61 taagtcgcgt gtgaaatctc caggctcaac ttggaaagtg cgcgcgaaac tggcgagctt
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga aggtaactga cactgagcag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007334
VERSION     KHUT01007334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           83 g           59 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcaga
       61 taagtctgat gtgaaacctt tcggcttaac cgaaagacgt cgttggatac tgtctgtctt
      121 gaggtaatga gaggagtgcg gaattctcgg tgtaatggtg gaatatgtag atatcgagag
      181 gaacatctgt ggcgaaagcg gcactctggc atttgcctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007335
VERSION     KHUT01007335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           85 g           57 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggcttta
       61 ccagtcagga gtgaaatccc ggagcttaac ttcggaattg cttttgaaac tgtaaggcta
      121 gagtatgtta gaggatggcg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg acataactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007336
VERSION     KHUT01007336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           80 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgca ggcggatttg
       61 taagtcagtg gtgaaagccc cgggctcaac ctgggaactg ccattgatac tgcaagtctt
      121 gagtatagtt gaggtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcgctaaa ctataactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007337
VERSION     KHUT01007337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           94 g           52 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggattaa ctgggcgtaa agggagcgca ggcggtttgg
       61 tcagtctgaa gtggaagccc ggggctcaac ctcggaagcg ctttggatac tgccagactt
      121 gagttcgtga gaggagagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatctgt ggcgaaagcg gctctctggc acgatactga cgctgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007338
VERSION     KHUT01007338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           97 g           45 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatca ctgggcgtaa agggggcgca ggcggtttgt
       61 taagtccgga gtgaaagccc gaggctcaac ctcggagggt ctttggaaac tggcagacta
      121 gagtacggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctctctggc ccgatactga cgctgaggcc cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007339
VERSION     KHUT01007339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           84 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgg
       61 caagacggat gtgaaatccc agggcttaac cttggaactg cattcttaac tgccaggcta
      121 gagtatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007340
VERSION     KHUT01007340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcccgg
       61 tcagtcggat gtgaaaacac gaggctcaac ctcgtgcctg cattcgatac tgccgggctc
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01007341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007341
VERSION     KHUT01007341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           83 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtac agggtgcgca ggcggtatgt
       61 taagtcaact gtcaaagacc tcagcctaac tgaggaccgg cagtagatac tggcgtacta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007342
VERSION     KHUT01007342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtctggc gtgaaaggct tcggctcaac cggagaaggc cgctggaaac tgtcgttctt
      121 gagggtcgaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggt cgacatctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007343
VERSION     KHUT01007343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           54 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggaataa ctgggcgtaa agggtgcgta gacggtttgt
       61 taagcttgtt gttaaattcc ccggcctaac tgggggcatg caatgagaac tggcatacta
      121 gaggatgaga gagaagagtg gaatttccgg agtagcggta aaatgcgtag atctcggaag
      181 gaacaccgat ggcgaaggca gctctttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007344
VERSION     KHUT01007344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           94 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggcggt
       61 caagtcaggg gtgaaatcct ccagctcaac tggagaagtg cctttgatac tggccgcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007345
VERSION     KHUT01007345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 taagtcgggt gtgaaagccc caggctcaac ctgggaactg cactcgagac tgcattgctg
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg actgcctggc ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007346
VERSION     KHUT01007346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           89 g           54 t
ORIGIN      
        1 tacggaggtt cctagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggattga
       61 taagtctaaa gtggaatccc ggggctcaac ctcggacgtg ctttggatac tgtcagtctt
      121 gagttcgtga gaggaaggcg gaattcaggg tgtagcggtg aaatgcttag atatcctgag
      181 gaacaccagt ggcgaaagcg gccttctggc acgaaactga cgctgaggct cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007347
VERSION     KHUT01007347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggccttg
       61 taagttagaa gtgaaatccc cgagctcaac tcgggaagtg cttttaaaac tgcagggctt
      121 gaatgtggga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ccaacattga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007348
VERSION     KHUT01007348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           88 g           43 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaacgg cgctccaaac taccgtgcta
      121 gaggaagaca gaggtaagcg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007349
VERSION     KHUT01007349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 tacagaggtc gcaagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggttgtg
       61 caagtcggtt gtgaaatccc cgggctcaac ctgggaactg cttccgagac tgtgcgacta
      121 gagatcggta gagggcggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccaat ggcgaaggca gccgcctggg cctgatctga cactgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007350
VERSION     KHUT01007350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggtcctt
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaactg cgcttgagac tggaggactt
      121 gaggacggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccaat ggcgaaggca gctctctgga ccgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007351
VERSION     KHUT01007351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           85 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggtatt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaatcg cattcgatac tgggtatcta
      121 gagtatgata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga tcaatactga cactaaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007352
VERSION     KHUT01007352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          100 g           43 t
ORIGIN      
        1 tacggagggg gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcggtcgtg
       61 cgcgtcagag gtgaaatccc ccggcttaac cggggacgtg cctctgatac ggcatggctt
      121 gagtccggga gaggggagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctccctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007353
VERSION     KHUT01007353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           95 g           41 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtccat
       61 taagtcggat gtgaaatctc acggcccaac cgtggaattg catccgatac tggtggactt
      121 gaggacggta gaggccggtg gaattcccgg tgtaccggtg aaatgggcag atatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ccgatcctga cgctgagacc cgaaagccag
      241 gggagcaaac cgg
//
LOCUS       KHUT01007354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007354
VERSION     KHUT01007354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           89 g           46 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ctcggaatca ctgggcataa agcgcgcgta ggtggcctga
       61 caagtcagtg gtgaaatccc atcgctcacc gatggaactg ccgctgatac tgtcgagctt
      121 gaggacagta ggggagagtg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggg ctgttcctga cactgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007355
VERSION     KHUT01007355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           99 g           38 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggg
       61 caagtcggag gtgaaagccc acggctcaac cgtggaagtg cccccgatac tgcttgtctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007356
VERSION     KHUT01007356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggttgtg
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaactg catttgagac tgcacggcta
      121 gaggatggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ctatttctga cactcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007357
VERSION     KHUT01007357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           94 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctatc gtgaaagtcc gaggctcaac ctcggatctg cggtgggtac gggcagacta
      121 gagtgatgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01007358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007358
VERSION     KHUT01007358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c          102 g           42 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggatggg
       61 taagtgggtc gtgaaagccc ttggctaaac tgggggagga cgatacagac tgctcgtcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007359
VERSION     KHUT01007359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           98 g           38 t
ORIGIN      
        1 aacgtagggg tcgagcgtta tccggaatta ctgggcgtaa agcgcgtgca ggcggcagca
       61 caagtcggac gtgaaagctc ctggctcaac tgggagaggc cgttcgaaac tgtgcagcta
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctgga ccgtgactga cgctgagacg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01007360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007360
VERSION     KHUT01007360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           79 g           56 t
ORIGIN      
        1 tacgagagcc ccaagcgtta tccggattta ctgggtgtaa agggtgtgta ggtggtttag
       61 ttagtcttgc gttaaagcct tgggctcaac tcaagaattg cgcaggaaac ggctaaacta
      121 gagagtgtga gaggtctgtg gaactcatgg tgtaggggtg aaatccgttg atatcatgag
      181 gaacaccgaa agcgaaggca acagactggc acatttctga cactgagaca cgaaagccag
      241 ggtagcgaat cgg
//
LOCUS       KHUT01007361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007361
VERSION     KHUT01007361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctttg
       61 taagttgggt gtgaaagccc agggctcaac cctggaagtg catccaaaac tgcgaggctt
      121 gagtcccgga ggggcccaga gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cgggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007362
VERSION     KHUT01007362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           83 g           62 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtgggttaa
       61 taagtcagtg gtgaaatacg gttgctcaac aatcgaggtg ccattgatac tgttaatctt
      121 gaaacaagtg gaggttgccg gaatggatgg tgtagcggtg aaatgcatag atatcatcca
      181 gaacaccgat tgcgaaggca ggtgactacg tttgatttga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007363
VERSION     KHUT01007363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 cacgtaaggt gcgagcgtta ttcggaatta ttgggcgtaa agggcacgca ggcggctttg
       61 caagcttggt gtgaaatctc agggctcaac cctgaaactg cattgagaac tgcatggcta
      121 gagttagtga ggggaaaccg gaattccagg tgtaggggtg aaatctgtag atatctggaa
      181 gaacaccaat ggcgaaggca ggtttccagc acataactga cgctcaggtg cgaaggtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01007364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007364
VERSION     KHUT01007364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtcgag
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgatac tgcccggcta
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cagatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007365
VERSION     KHUT01007365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          101 g           48 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggttttg
       61 tttgtccgct gtgaaagtcc acggcttaac tgtggagagt cggcggaaac ggcaagactt
      121 gaggctggga ggggttggtg gaattccctg tgtagcggtg aaatgcgtag atacggggga
      181 gaacactcgt ggcgaaggcg gccagctggc ccagtcctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007366
VERSION     KHUT01007366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           98 g           47 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcggat gtgaaagctc gaggcttaac ctcgggtctg catccgatac gggcagacta
      121 gaggcaggta ggggagaacg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007367
VERSION     KHUT01007367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggcgatctt
      121 gagccactta ggggtcagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacaccaat ggtgaaaaca gctgactggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007368
VERSION     KHUT01007368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           96 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggttcgc
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaattg ccttggatac tggcgggcta
      121 gaagagggta ggggctgcgg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg cgtggctgga ccctccttga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007369
VERSION     KHUT01007369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtctgaa gtgaaatcct ccagcttaac tggagaacag ctttggatac tggtcgtctc
      121 gagtggggta ggggcgtgtg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaagcg acacgctgga cccttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007370
VERSION     KHUT01007370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           85 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatggt
       61 taagtccaag gtgaaaggtg ggagctcaac tcccacactg cctgggatac tggccgtctt
      121 gagtggcgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cgcaaactga cgctgagata cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01007371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007371
VERSION     KHUT01007371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           82 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggactgt
       61 taagtcagcg gtgaaagccc acagctcaac tgtggaactg ccgttgatac tgacagtctt
      121 gagtacaagt gaagttggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacaccgat tgcgaaggca gctggctaac ttgtaactga cgctgaggga cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007372
VERSION     KHUT01007372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           61 c           93 g           40 t
ORIGIN      
        1 gacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg ctgggtatac tggcgatctt
      121 gggccggtca ggggcagtcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggcgaaagca gactgctggg accgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007373
VERSION     KHUT01007373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgc
       61 caagtctggg gtgaaatcct cccgcttaac gggagaactg cctcagatac tggcgatctc
      121 gagggggata ggggcatgcg gaactacggg tggagcggtg aaatgcgttg atatccgtag
      181 gaactccggt ggcgaaggcg gcgtgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007374
VERSION     KHUT01007374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           85 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agcgaacgca ggcggattgt
       61 taagtcagaa gtgaaaggtt ggagctcaac tctaacattg cttccgagac tggcagtctt
      121 gagtgccgga gaggatgatg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca atcatctgga cggaaactga cgctgaggtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01007375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007375
VERSION     KHUT01007375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           98 g           39 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtacctt gtgaaatccc ccggctcaac cggggaacgg ctgggtatac tggcggtctt
      121 gggcgactca ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtccgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007376
VERSION     KHUT01007376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggacgct
       61 taagtcaggg gtgaaaggcc ggggctcaac cccggaacgg cccttgatac tgggcgtctg
      121 gagtccggaa gaggttggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga ggcgaaggcg gccaactggt ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007377
VERSION     KHUT01007377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaga
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catttgaaac tgtttaactg
      121 gagtgcggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007378
VERSION     KHUT01007378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           53 c          102 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggtggttcga
       61 tgcgtcgtgc gtgaaagacc ctggctcaac tggggggtgt cgtgcgatac ggtcgaactg
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cactcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01007379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007379
VERSION     KHUT01007379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           87 g           46 t
ORIGIN      
        1 gacggaccac ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgtag
       61 taagtcgggt gtgaaatccc ccagcttaac tgaggaattg cgcccgaaac tgctgcgctc
      121 gagggagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctcaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007380
VERSION     KHUT01007380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttag
       61 caagtcagat gtgaaagccc ggggctcaac tccggaattg catttgaaac tgctaggcta
      121 gagtgcagta gaggatggcg gaatacccag tggagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gccatctggg ctgtaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007381
VERSION     KHUT01007381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtctgg
       61 aaagtctggt gtgaaaactt gaggctcaac ctcaagctgt cgctggatac tgtcagactt
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gcactctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007382
VERSION     KHUT01007382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           48 c          102 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtctgct gtgaaagccc ggagcttaac tccggagggt cggtggatac tgccaatctt
      121 gaagggacta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gttatcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007383
VERSION     KHUT01007383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtttggg gtgaaatgcc acggctcaac cgtggaactg cctcaaatac tgacggtctc
      121 gagggaggta ggggcgagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaactccggt ggcgaaggcg gctcgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007384
VERSION     KHUT01007384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          105 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtccga
       61 taagggggcg gtgaaagccc ggcgcttaac gccgggtcgg ccgtgccgac tgtcggactg
      121 gagcactgta gaggcaggta gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaataccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007385
VERSION     KHUT01007385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggcggactta
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg catttgatac tgtaagtctt
      121 gaatgtggta gaggttactg gaattgctgg tgtagtggtg aaatacgtag atatcagcag
      181 gaacaccgga ggcgaaggcg ggtaactggg ccaacattga cgctgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007386
VERSION     KHUT01007386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           87 g           47 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcag
       61 taagtagggt gtgaaatccc tcggcctaac cgaggaaccg cgccctaaac tactgtgctc
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007387
VERSION     KHUT01007387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattca ttgggcgtaa agcgcatgca ggcggcttgg
       61 taagtcatcg gtgaaatccc ccggctcaac cggggggggt ccggcgaaac tgccaggctt
      121 gaggtcggga gggggaagcg gaattccggg tgtagtggtg gaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ccgaatctga cgctgagatg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01007388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007388
VERSION     KHUT01007388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           86 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ttgggcgtaa agcgcgcgca ggcggatcaa
       61 taagtcaggt gtgaaatctc gaagctcaac ttcgaaactg cgcctgaaac tattagtcta
      121 gagtgttgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctacctgga caactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007389
VERSION     KHUT01007389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccttg
       61 caagtcgggt gtgaaagccc tcggctcaac cggggaagtg cactcgaaac tgcagggcta
      121 gagtctcgga gaggatggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007390
VERSION     KHUT01007390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcggta
       61 gtagtcaggc gtgaaattcc tgggcttaac ctggggactg cgcttgatac gctattgcta
      121 gaggacggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgttactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007391
VERSION     KHUT01007391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtggatagg
       61 taagtcagcg gtgaaatact ttcgcttaac gaaggaactg ccgttgatac tatttatctt
      121 gaatacagtg gaggtgggcg gaatgtgcca tgtagtggtg aaatacttag atatggcaca
      181 gaacacctat tgcgaaggca gctcgctaca ctgtcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007392
VERSION     KHUT01007392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           47 c           97 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcggtt gtgaaaactg gaggcttaac cttcagcgtg caatcgatac gggcagactt
      121 gagttcggta ggggagtctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007393
VERSION     KHUT01007393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           82 g           52 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ctgggcgtaa agggtgcgta ggcggcatac
       61 taagtcaact gtaaaattct tcggcctaac cggaggctcg cagtagaaac tgatatgctt
      121 gaggttggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007394
VERSION     KHUT01007394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           98 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcaggc
       61 caagtcggga gtgaaagccc ggggctcaac cccggaatgt ctttcgaaac tggccagctg
      121 gagtgcggaa gaggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007395
VERSION     KHUT01007395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattta ctgggcgtaa agggtgtgta gacggcatgt
       61 taagcttgtt gttaaatttc ccggcctaac tgggaatatg cgatgagaac tggcgtgcta
      121 gaggatggaa gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007396
VERSION     KHUT01007396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggccag
       61 taagcgtctt gtgaaatccc ccggctcaac cggggaactg cggggcgaac tgctggtctt
      121 gaggaagcta gaggccggta gaacgattgg tggagcggtg gaatgcgtag atatcaatcg
      181 gaatgccgaa agtgaagaca gccggctggg gctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007397
VERSION     KHUT01007397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 gacggaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggctcag
       61 aaagttgaat gtgaaagccc tcggcttaac cgaggaattg catccaaaac tactgggctt
      121 gagtgagaca gaggtgagcg gaactcacgg tggagcggtg aaatgcgttg atatcgtgag
      181 gaacaccggt ggcgaaagcg gctcactggg tctttgctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007398
VERSION     KHUT01007398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcggtg
       61 taagtcaggt gtgaaagccc tcagcttaac tgaggaagtg cacttgatac tgcatcgctt
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga cgacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007399             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007399
VERSION     KHUT01007399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 cgagtcgagt gtgaaacctt caggctcaac ctggagatgc cactcgatac tgccatggct
      121 tgagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007400             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007400
VERSION     KHUT01007400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           99 g           49 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtttg
       61 aaagtcggag gtgaaaggct ggggctcaac cccatgcagt gcctccgata cttcaagtct
      121 tgagtggggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gcctttactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007401             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007401
VERSION     KHUT01007401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           48 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggctcgta ggcggcttag
       61 caagtcaggt gtgaaacctc caggcttaac ctggagacgc catttgagac tgctatggct
      121 agagtgcggt aggggaccat ggaatctccg gtgtagcggt gaaatgcgca gatatcggag
      181 ggaacaccag tggcgaaggc ggtggtctgg gccgtaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01007402             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007402
VERSION     KHUT01007402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ttgggcttaa agagcacgta ggcggcgaca
       61 taagtcgggg gtgaaatact aacggctcaa ccgtagaatt gccttcgata ctgtgtcgct
      121 cgaggaagat aggggcgagt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccag tggcgaaagc gactcgctgg gtcttatctg acgctgaggt gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007403             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007403
VERSION     KHUT01007403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           52 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ttgggcttaa agcgcgcgta ggcggcggcg
       61 taagtggagg gtgaaatcct atcggctcaa ccgtgggttt gcctttcaaa ctgcgttgct
      121 tgaggaagac aggggcacgt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacgtgctgg atcttatctg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007404             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007404
VERSION     KHUT01007404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          102 g           41 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatca ttgggcgtaa agggctcgta ggcggactgg
       61 caagtctggc gtgaaatctc caccactcaa cggggagggg ccgtcggaaa ctgccagtct
      121 ggagtacggt agaggaccat ggaattcctg gtgtagcggt ggaatgcgta gagatcagga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgttactg acgctgagga gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007405             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007405
VERSION     KHUT01007405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           63 c          101 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcgggcgac
       61 cgcgtcgatg gctgaaagcc cccggcttaa ccggggaacg ggcctcgata cgggtcgcct
      121 cgaggcgagg agggggacct ggaacgtccg gtggagcggt gaaatgcgtt gagatcggac
      181 ggaacaccga cggcgaaagc aaggtccggg actcgtactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007406
VERSION     KHUT01007406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggatgaa
       61 taagtccgag gtgaaagacg gtcgcttaac gatcgcagtg ccttggatac tgtttatctt
      121 gagtacagat gaggctgatg gaatgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gtcagctaaa ctggcactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007407
VERSION     KHUT01007407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggatata ctgggcgtaa agcgcacgta ggcggcacga
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgaaac tgtcgcgctt
      121 gaattctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga cagacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007408
VERSION     KHUT01007408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 gacgaagggt cctagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggtggctcaa
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg cacttgatac tgttaagctt
      121 gagtatggga gagggtcgtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcggcctggc ccaatactga cactgagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007409
VERSION     KHUT01007409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggacgat
       61 taagtccggg gtgaaagacg acggctcaac cgtcgcagtg ccttggatac tgattgtctt
      121 gagtacagat gaggctgatg gaatgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gtcagctaag ctggaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007410
VERSION     KHUT01007410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           88 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctaag
       61 taagctagat gtgaaagccc tgggctcaac ctgggaactg catatagaac tgcttagcta
      121 gagtgcagaa gaggagtgtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacatcagt ggcgaaggcg acactctggt ctgacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007411
VERSION     KHUT01007411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacagagagt gcgaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcgcca
       61 taagtgcggg gtgaaatccc acggcttacc cgtggaactg cctggtagac tgtggtgctt
      121 gaggatggta ggggagagcg gaactcctgg tggagcggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007412
VERSION     KHUT01007412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 caagtcctgt gtgaaatccc ccggctcaac tggggaactg cacaggaaac tgcttggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007413
VERSION     KHUT01007413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgggtc ggtggtgcct
       61 taagtcatgc gtgaaagctc tcggcttaac cgggagaggt cgtgtgaaac tggggcgctg
      121 gaggttggga gaggcagacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gtctgctagc ccacacctga cactcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007414
VERSION     KHUT01007414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           83 g           51 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattca ttgggcgtaa agagtccgta ggcggtccgt
       61 aaagttgggt gtgaaatcct acagcttaac tgtagaactg cattcaaaac tcacggacta
      121 gagtgtgaga ggggtgaagg gaattcccag tgtaagggtg aaatctgcag agattgggaa
      181 gaacaccagt cgcgaaggcg cttcactggc tcactactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007415
VERSION     KHUT01007415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggtgggttta
       61 caagtcggaa gtgaaatctt gaggctcaac ctcgaggctg ctcccgaaac tgtgagccta
      121 gggactggta gaggctggta gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaatacctgt ggcgaaggcg gccagctggg ccagatccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007416
VERSION     KHUT01007416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           82 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttga
       61 taagtcagtg gttaaagact cttgctcaac gagagggatg ccattgatac tgtcagactt
      121 gagtattgta gaggtaggcg gaattggtag tgtagcggtg aaatgcatag atactaccag
      181 gaacaccgat tgcgaaggca gcttactggg caataactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007417
VERSION     KHUT01007417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgt
       61 taagtcctgt gtgaaagccc cgggctcaac ccgggaagtg cgcaggatac tggcgagctg
      121 gagtggcgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga cgttgactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007418
VERSION     KHUT01007418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           65 c          106 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccgc
       61 cgcgtccggg gtgaaatccc gtggctcaac cacgggacgg cctcggatac gggcgggctg
      121 gaggggggta ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccccacctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007419
VERSION     KHUT01007419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           81 g           60 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtttgta ggcggttaat
       61 caagtcaggg gtgaaatccc agagctcaac tctggaattg cctttgaaac tgattagcta
      121 gagaatgtta gaggaaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gctttctggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007420
VERSION     KHUT01007420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctctgatac tggggatctc
      121 gaggggggta ggggcctgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcaggctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007421             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007421
VERSION     KHUT01007421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          103 g           39 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taggttccac tgtgaaaggc aggggctcaa cccctggatg gcggggaaaa ccggcaggct
      121 ggagagaggc agaggaaggt ggaatggctg gtggagcggt gaaatgcgta gagatcagcc
      181 ggaacaccca aggcgaaggc agccttctgg gcctcatctg acgctgtggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007422
VERSION     KHUT01007422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctca
       61 tttgtccggg gtgaaatcct tgagcttaac tcaagaagtg ccccggagac ggtgagacta
      121 gagttttgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaagcg gcgacctgga caatgactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007423
VERSION     KHUT01007423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcttt gtgaaatccc ccggctcaac cggggaactg cttggcatac tggcgacctt
      121 gagccaccta ggggcaactg gaacgaacgg tggagcggtg aaatgcgtag atatcgttcg
      181 gaacgccgaa ggtgaaaaca ggttgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007424
VERSION     KHUT01007424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttacc
       61 taagtcttct gtgaaatcta cgggcttagc ctgtaaagtg cagaggaaac tgggtgactt
      121 gagtgtcgga gaggataggg gaatacccgg tgtagaggtg aaattcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007425
VERSION     KHUT01007425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           37 c           82 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttaaa
       61 taagttagat gtgaaaggcc tgagcttaac ttgggaactg catataatac tgtttaacta
      121 gagtactgta gagggtagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcagt ggcgaaggcg tctacctgga cagaaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007426
VERSION     KHUT01007426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           94 g           43 t
ORIGIN      
        1 tacagggggc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggacgag
       61 aaagtgttct gtgaaatccc ccggctcaac cggggaatgg cggggcaaac ttctcgtctt
      121 gaggcaagta gaggctgtcg gaacgctagg tggagcggtg aaatgcgtag agatctagcg
      181 gaacgccgaa ggtgaagaca ggcagctggg cttgtcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007427
VERSION     KHUT01007427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           80 g           61 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggctttg
       61 taagtcagcg gtgaaatctt agagctcaac tctgaaactg ccgttgatac tacttggctt
      121 gagtatggaa gaggttgatg gaatttcagg tgtagcagtg aaatgcgtag atatctgaaa
      181 gaacaccagt ggcgaaggcg gtcaactggt ccattactga cactaaagca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007428
VERSION     KHUT01007428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttaat
       61 taagttagat gtgaaatccc tgggcttaac ctaggaattg catttaaaac tggttgacta
      121 gagtgtggta gagggaagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccactactga cactgaggcg cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007429
VERSION     KHUT01007429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           85 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgta ggcggattgg
       61 caagtcagag gtgaaatcct gcagcttaac tgcagaactg cctttgaaac tgccagtctt
      121 gagtacggaa gagagaagcg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt tgcgaaggcg gcttcttggt ccgttactga cgctcaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007430
VERSION     KHUT01007430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtcatg
       61 caagtgtgat gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac ggtgtgactg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttgatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007431
VERSION     KHUT01007431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          103 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggcttgc
       61 taagtcttgt gtgaaatctc ccggctcaac tgggagggtg catgggaaac tggcaggctt
      121 gagactcgaa ggggggtgcg gaattcccgg tggagcggtg gaatgcgttg atatcgggag
      181 gaacacccga ggcgaaggcg gcaccctggt cgaggtctga cgctgaagcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007432
VERSION     KHUT01007432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacgtagggt gctaacgttg ttcggaatta ttgggcgtaa agcgcgtgta ggtggttatg
       61 caagtcagat gtgaaatccc tgggcttaac cgaggaagtg catctgaaac tgcatagcta
      121 gaggacggta gagggaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gcctcctggg ccgttcctga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007433
VERSION     KHUT01007433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgca ggcgggatag
       61 caagtcagag gtgaaatcct acagcttaac tgtagaactg cctttgatac tgttattctt
      121 gagttcggga gagagagacg gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaacaccagt tgcgaaggcg gtctcttggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007434
VERSION     KHUT01007434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           90 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcgttttct
       61 taagttacct ttcaaagacc ggggctcaac tccggagcgg ggggtaatac tgggaaaatt
      121 gaaaggtttc ggggctactg gaactgtcgg tgtaggagtg aaatccgttg atatcgacag
      181 gaacaccgag ggcgaaggcg ggtagctagg aacattttga cgctgaggaa cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01007435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007435
VERSION     KHUT01007435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggattta ctgggtgtaa agggcgtgta ggcgggtttg
       61 caagtcggat gtgaaattcc caggcttaac ttgggcgggt catccgaaac tgcgaatctt
      121 gagtactgga gaggatagtg gaattcctag tgtagcggta aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctatctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007436
VERSION     KHUT01007436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacagagact ccaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggtctgg
       61 tgtgtcgagg gtgaaatcct gcagcttaac tgtagaatgg ccttcgaaac taccagacta
      121 gagtatcgga gagggaagcg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccgaa ggcgaaggca gcttcctgga cgattactga cgctcaggca cgaaagcatg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01007437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007437
VERSION     KHUT01007437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggcctgt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgagac tggctagcta
      121 gagtctggta gaggggtgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg acaccctgga ccaagactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007438
VERSION     KHUT01007438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgct
       61 taagcgagat gtgaaatccc cgggcttaac ctgggcactg catttcgaac tgggcgactg
      121 gagttttgga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gctctctggc caaaaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007439
VERSION     KHUT01007439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtcttt
       61 taagtctgat gtgaaatctc tcagctcaac tgggagcagc cattggaaac tggatgactg
      121 gagtctgaca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg tcagtactga cgctgaaccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007440
VERSION     KHUT01007440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           97 g           41 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggttagg
       61 gaagtcaaag gtgaaagccc ccggctcaac cggggaattg cctgtgaaac cacctgactg
      121 gagtgctgga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacatctgt ggcgaaggcg gctttctgga cagacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007441
VERSION     KHUT01007441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtcgct
       61 taagtctgat gtgaaatccc ggggctcaac cccggaaggg cattggaaac tgagcgacta
      121 gagtgccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007442
VERSION     KHUT01007442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           79 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggctgg
       61 taagtccgtg gtgaaatccc cgagcttaac ttgggaactg ccgtggatac tatcagtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat agcgaaggca gcttactaca cgatcattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007443
VERSION     KHUT01007443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggcc gtgaaaactc ggggctcaac cccgagcctg cggtcgatac gggcagacta
      121 gagtgctgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007444
VERSION     KHUT01007444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgg
       61 caagtcgggg gtgaaatctt tcggctcaac cggaaaagtg ccttcgatac tgccaggctc
      121 gaggaaggta ggggtcacgg gaacgaccag tggagcggtg aaatgcgttg agattggtag
      181 gaacgcctgt ggcgaaagcg cgtgactgga ccttccctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007445
VERSION     KHUT01007445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccatt
       61 caagttagat gtgaaagccc tgggctcaac ctaggaattg catttgatac tgtttggctt
      121 gagttcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007446
VERSION     KHUT01007446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 tacagggggc gcgagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcggactgt
       61 taagtcgcat gtgaaagccc ggggctcaac cccggaagtg catgcgaaac tggcagtctt
      121 gaatacggga gaggtgagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactggc ccgatattga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007447
VERSION     KHUT01007447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgcat
       61 caagtatgat gtgaaatccc cgggcttaac ctgggaactg catcatagac tgatgaacta
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007448
VERSION     KHUT01007448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtgggg
       61 taagtctgat gtgaaatctc ctggcttaac tgggaggggt cattggaaac tgccgtactt
      121 gaggcgatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg acaccctggc attagcctga cgctcatgtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007449
VERSION     KHUT01007449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgaga
       61 ttagtcgggt gtgaaatctc cgggcttaac ccggagggtg cgctcgaaac tgtctcgcta
      121 gagggtggga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga ccactcctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01007450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007450
VERSION     KHUT01007450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggtgtaa agggcaggta ggtggtcatg
       61 aaagtccatt gtgaaatcct agagctcaac tctagaactg cagtggaaac tccatggctt
      121 gagtgtcgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaataccagt ggcgaaggcg cctcactggc cgacaactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007451
VERSION     KHUT01007451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           83 g           55 t
ORIGIN      
        1 tacagagacc tcgagcgttg accggattta ttgggcgtaa agcgtgcgca ggcggtttcg
       61 taagtcattt gttaaatctt caggctcaac ctggaggcag cgggtgatac tgcgagacta
      121 gaggatggga gaggaaggcg gaatgtctgg tgtaggggta aaatccgtta atatcagata
      181 gaacaccaaa tgcgaaggca gccttctgga acactcctga cgctgttgca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01007452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007452
VERSION     KHUT01007452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           85 g           52 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 taagtctgtt gtgtaagctc ctggcttaac cgggagaggt caacagatac taccaggctt
      121 gagtatagga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtaactgga ctattactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007453
VERSION     KHUT01007453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           94 g           40 t
ORIGIN      
        1 tacgagtggt ccaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcgggccag
       61 aaagcgtctt gtgaaatccc ccggctcaac cggggaacag cagggcgaac tactggtctt
      121 gaggaaggta gaggccggca gaacgatcgg tggagcggtg gaatgcgtag agatcgatcg
      181 gaatgccaaa ggtgaagaca gccggctggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007454
VERSION     KHUT01007454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           92 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggtagat
       61 taagctggag gtgaaaagcc tgggctcaac ctgggaccag cctccaggac tgatgtgcta
      121 gagtagggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ctcatactga cgctgaggcg cgaaagcatg
      241 ggaagcaaac agg
//
LOCUS       KHUT01007455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007455
VERSION     KHUT01007455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           85 g           49 t
ORIGIN      
        1 tacggagact acgagcgtta ctcggattta ctgggcgtaa agggtgcgta ggcggtcaga
       61 tgtgttaggt gtgaaagcct ggggcttaac tccagaattg cgcctaaaac tatctgactc
      121 gagcctcgga gaggcaagcg gaattcctag tgtagcagtg aaatgcgtag atattaggag
      181 gaacaccaaa ggcgaaggca gcttgctgga cgagatctga cgctgaagca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01007456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007456
VERSION     KHUT01007456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           83 g           55 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagtacgta ggcggtcttg
       61 taagacaatg gttaaatctc cgagctcaac tcggaacctg ccgttgttac tgcaagacta
      121 gtcgatagca ggggcaagcg gaattctcgg tgtaggagtg aaatccgttg agatcgagaa
      181 gaacaccaat ggcgaaggca gcttgctagg ttatttgaga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007457
VERSION     KHUT01007457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           88 g           46 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agagggcgta ggcggggatg
       61 caagtcagat gtgaaagccc ggggctcaac cccggaacgg catttgaaac tgcatttcta
      121 gaggataggc ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttaggcctga cgctgaggcc cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007458
VERSION     KHUT01007458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggatgat
       61 caagtcaggt gtgaaatccc tgggctcaac ccaggaactg catctgaaac tggtcgtctt
      121 gagtactgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagtcactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007459             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007459
VERSION     KHUT01007459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcaggg
       61 taagttcgat gtgaaagctc ggagctcaac tccgaaatgg cattgaatac tctcctgctt
      121 agagggtcgg aggggagact ggaattctcg gtgtagcagt gaaatgcgta gagatcgaga
      181 ggaacaccgg tggcgaaggc gagtctctgg acgacacctg acactgaggc acgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007460
VERSION     KHUT01007460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 gacatatggg gcaagcgtta ttcggaatta ctaggcgtaa agcgcgtgta ggcgggtgcg
       61 caagtctgcc gtgaaatctc ccggcttaac tgggaggggt cggcggatac tgcgtgtcta
      121 gagttgggta gggggtaccg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaagca ggtacctggg ccttgactga cgctgagacg cgaaagctgg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007461
VERSION     KHUT01007461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacggaaggt gcaagcgtta atcggaatca ctgggcgtaa agcgcacgta ggcggccttg
       61 taagtcggat gtgaaagccc tcggctcaac cggggaattg cattcgaaac tgcctggctg
      121 gagtcctgga gagggtggcg gaattcccgg tgtaggagtg aaatccgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga caggtactga cgctgaggtg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007462
VERSION     KHUT01007462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           44 t
ORIGIN      
        1 tacagggggc gcgagcgttg ctcggaatca ctgggcgtaa agcgaacgca ggcggaccgt
       61 taagtcgaat gtgaaagccc ggggctcaac tccggaagtg cattcgaaac tggcggtctt
      121 gagtacggga gaggtgggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcccactggc ccgatactga cgctcatgtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007463
VERSION     KHUT01007463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctcg
       61 taagtcagac gtgaaatccc tcggctcaac cgaggaactg cgtctgatac tgcgaaacta
      121 gagtgtcgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg accatctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007464
VERSION     KHUT01007464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           57 c           76 g           50 t
ORIGIN      
        1 tcccagcagt ccaagtcgca accacatttg ttgggtctaa aacatccgta gctcgcctcg
       61 taagtctgtc gtgaaatcct acctcttaag ggtagaactg cgataggtac tgcgaggcta
      121 gggaccggga gacgtaacga gtacgcatag agtaccggta aaatgggttg atcctatgtg
      181 gactaacaac agcgaaggca cgttacgagc acggatccga cagtgaggga tgaaggctag
      241 gggcgcaaag tgg
//
LOCUS       KHUT01007465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007465
VERSION     KHUT01007465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 tacgaaggtc cctagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggtggctttg
       61 caagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cactcgaaac tgcatagctt
      121 gagtacggta gaggtgagtg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacatcagt ggcgaaggcg gctcactgga ccagtactga cactgagaag cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01007466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007466
VERSION     KHUT01007466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggctatg
       61 caagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgagac tgcattgctc
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cactcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007467
VERSION     KHUT01007467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaaacgtta tccggaatca ctgggcgtaa agcgcgtgta ggtggttttg
       61 taagtggacg attaaatacc gatgcttaac atcgggactg ttgtctatac tgcatgactt
      121 gagaatggta ggggcaggcg gaactttggg tgtaggggtg aaatccgtag atatccaaag
      181 gaacaccaat ggcgaaggca gcttgctgga ccatttctga cactgagacg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007468
VERSION     KHUT01007468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggaggttggc
       61 aaagtcgggt gtgaaatctc accgcttaac ggtgaaattg cgctcgatac tggttgacta
      121 gagtatcgga ggggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaac ggcgaaggca gctttctgga cgataactga ctctgaggca cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007469
VERSION     KHUT01007469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           41 c           76 g           58 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ctgggcgtaa agcgcgcgta ggtggtgcaa
       61 taagttggaa gtgaaatccc aaagctcaac tttggaactg ctttcaaaac tattacgcta
      121 gagtttagta gaggatgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccgat ggcgaaagca atcatctggg ctaatactga cactaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007470             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007470
VERSION     KHUT01007470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           55 c           79 g           49 t
ORIGIN      
        1 ccccagcgcc acaagtggtt atcacgatta ttgggcctaa agcgttcgta gccggtttgg
       61 taaatctctt gtgaaattgt tcggcttaac cggacagcgt gcaggagaca ctgccaaact
      121 cgagaccggg aggcgtatga agtattccat ggggactggt aaaatgggat aatccatgga
      181 agactaccga tggcgaaggc atcatacgag aacggatctg acggtgagga acgaaagcca
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01007471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007471
VERSION     KHUT01007471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           96 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccga
       61 tcagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac tgtcggcctc
      121 gagggggata ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007472             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007472
VERSION     KHUT01007472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          101 g           44 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattca ctgggcgtaa agggcgcgca ggcggctacg
       61 taagtccggc gtgaaatccc acggctcaac tgtggaattg cgtcggatac tgtgttggct
      121 tgaggacggt agaggcttct ggaactagcg gtgtagcggt gaaatgcgca gatatcgcta
      181 ggaacgccgg tggcgaaggc gggaggctgg gccgatcctg acgctgaggc gcgaaagcta
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01007473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007473
VERSION     KHUT01007473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           87 g           47 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtcatg
       61 caagtcggat gtgaaatccc ggagcctaac tccggaacgg cattcgaaac tgcagggctc
      121 gagatctgga gaggtgattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaagcg aatcactgga cagaatctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007474             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007474
VERSION     KHUT01007474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           98 g           48 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggatctg
       61 tgtgtctggc gtgaaatctc caccacttaa cggggagagg tcgctggaaa ctgcagatct
      121 ggagtttgtt agaggaccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gacaaaactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007475             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007475
VERSION     KHUT01007475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           84 g           51 t
ORIGIN      
        1 tacggagagt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggatcgt
       61 caagtcagat gtgaaattcc gaagctcaac ttcggagctg catttgaaac tagcgatcta
      121 agagggttga cggagaaaac ggaattccac gtgtagcggt gaaatgcgta gatatgtgga
      181 agaacaccgg tggcgaaagc ggttttctag ttgatacctg acgctgaggc gcgagagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007476
VERSION     KHUT01007476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggttact
       61 caagtcaggg gtgaaatcct gaggctcaac ctcagaattg cctttgaaac tgtgtgactg
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagcggtg aaatgcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01007477             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007477
VERSION     KHUT01007477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           61 c           96 g           38 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgc
       61 taagtgtccg cgtgaaatcc cacggctcaa ccgtggaacg gccgggcata ctgacgacct
      121 tgaggcactc aggggcgagc ggaacgaacg gtggagcggt gaaatgcgta gatatcgttc
      181 ggaacgccga cggtgaaaac agctcgctgg gggtgtcctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007478
VERSION     KHUT01007478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggtggtttgg
       61 taagtcagtg gtgaaatcgt ccggcccaac cggacacgtg ccaatgatac tgccgaactc
      121 gagtgcgtga ggggtggggg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccaaa gccgaaggga ccccactggt acgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007479
VERSION     KHUT01007479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggccgc
       61 aaagtcaggg gtgaaatcct ccagcttaac tggagaagtg cctttgatac tggcggtctt
      121 gaggaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcgtgctgga ccttttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007480
VERSION     KHUT01007480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggcgatctt
      121 gggccaccta ggggtcaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccggc ggcgaaagcg ggtgactgga ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007481
VERSION     KHUT01007481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 taagttggaa gtgaaatccc atggcttaac catggaattg ctttcaagac tggagagcta
      121 gagtatggta gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007482
VERSION     KHUT01007482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttcgt
       61 taagttggat gtgaaagccc tgggctcaac ccaggaagag catacaaaac tggcgagctt
      121 gagtacagaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007483             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007483
VERSION     KHUT01007483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           51 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttag
       61 aaagtcaggt gtgaaacctc caggctcaac ctggagtcgc cacttgatac ttctatgact
      121 agagttcggt aggggaacac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgttctgg gccgaaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007484             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007484
VERSION     KHUT01007484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           98 g           48 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggtgtaa agggtgcgta ggcggttcgt
       61 taagtctgat gtgaaagccc tgggctcaac tcaggaatgg cattggaaac tggcgagctt
      121 ggagtactgg agagggaagc ggaattcctg gtgtagcggt gaaatgcgta gataccagga
      181 ggaacaccgg tggcgaaggc ggcttcctgg ccaagtactg acgctgaggc acgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007485
VERSION     KHUT01007485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggctcgg
       61 taagtccgct gtgaaagtcc agggctcaac cctggaatgc cggtggaaac tgtcgagcta
      121 gagtccggaa gaggtgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcactggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007486
VERSION     KHUT01007486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           47 t
ORIGIN      
        1 tacgtacgag gcgagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggtggctttt
       61 taagtagggc gtgaaatctc ccggcctaac tgggaggggt cgctctagac tggggagctt
      121 gaggctggca gaggtaggtg gaattcctgg tgtagtggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcctactggg ccagctctga cgctgaggcg cgaaagctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007487
VERSION     KHUT01007487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           97 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattga ctgggcgtaa agggcgcgta ggcggatagt
       61 acagtcaggc gtgaaattcc tgggctcaac ctgggggctg cgtttgatac gtattgtctg
      121 gagttcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007488
VERSION     KHUT01007488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           86 g           55 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatga ctgggcgtaa aggatgtgta ggcggctata
       61 ttagtcgagc gtgaaagccc cgggcttaac ctgggaactg cgttcgatac tgtatagctt
      121 gagaccggga gaggaaagcg gaatttctaa tgtagaggtg aaattcgtag atattagaag
      181 gaacatcaga ggcgaaggcg gctttctgga ccggtactga cgctgagaca tgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007489
VERSION     KHUT01007489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggtggcattc
       61 caagtctggt gtgaaagccc acggctcaac cgtggaactg cattggaaac tggaaagctc
      121 gagtattgga gggggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggc caattactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007490
VERSION     KHUT01007490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggttgtagaa
       61 taagtttttt gtgaaatccc cgggcttaac ctgggaactg cagagaatac tgttttacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007491
VERSION     KHUT01007491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgag
       61 caagtctagg gtgaaatcct ccggcttaac cggagaagtg ccttagatac tgcttgtctc
      121 gagggaggta ggggtcatcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gatgactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007492
VERSION     KHUT01007492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           90 g           43 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtcccg
       61 aaagtcgaag gtgaaagctc ccggcccaac tgggagaggc ccttcgaaac ttcgggactt
      121 gaaggcggga gaggcaaatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaagcg atttgctggc ccgcccttga cgctaaggcg cgaaagctag
      241 ggtagcgacc ggg
//
LOCUS       KHUT01007493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007493
VERSION     KHUT01007493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           83 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggcttcg
       61 taagtcagtg gtgaaagccc gcggctcaac cgtggaactg ccattgaaac tgcaaagctt
      121 gaatctggtt gaagtgggtg gaacatgaca tgtagcggtg aaatgcttag atatgtcgta
      181 gaacaccgat tgcgaaggca gcccactaag ccaacattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007494
VERSION     KHUT01007494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           93 g           46 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggtgata
       61 ttagtcaagc gtgaaagccc cgggcttaac ccgggaatag cgcctgaaac ggtatgacta
      121 gaggacgaga gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacatcaga ggcgaaggcg gctttctggc tcgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007495
VERSION     KHUT01007495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggtcttt
       61 taagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tgggagactt
      121 gagttcggta gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007496
VERSION     KHUT01007496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcaagg
       61 taagtctgat gtgaaatctc cgggctcaac tcggaaatgg cattggaaac tatctagctc
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007497
VERSION     KHUT01007497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggccgcgctg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgcggtgctt
      121 gaggactgga gaggagattg gaattcatgg tggagcagtg aaatgcgtag atatcatgag
      181 gaacgctagt ggcgtaggcg aatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007498
VERSION     KHUT01007498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtctga
       61 taagtctgct gtgaaagtct agggctcaac cctggaaagc cggtggatac tgtcagacta
      121 gagtgcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007499
VERSION     KHUT01007499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           49 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgtctt gtgaaatccc atcgctcacc gatggaactg ctggacatac tggcgatctt
      121 gagccactta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007500
VERSION     KHUT01007500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggg
       61 ccagtccggg gtgaaatcct gcagctcaac tgtagaagtg ccttggatac tgcccggctc
      121 gagggaggta ggggtgggtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acccactgga cctcttctaa cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007501
VERSION     KHUT01007501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           83 g           50 t
ORIGIN      
        1 gacgtaccac ccaaacgtta ttcggaatca ctgggcttaa agggtccgta ggcggtcagg
       61 aaagttgggt gtgaaatacc tcagctcaac tgaggaattg cgctcaaaac tacctggctt
      121 gagagagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcattg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgatgga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007502
VERSION     KHUT01007502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c          100 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatggt
       61 gcagtcagac gtgaaattcc tgggcttaac ctgggggctg cgtttgagac gcatggtcta
      121 gagtttggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt cctggactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007503
VERSION     KHUT01007503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agcgcgtgta ggcggccaga
       61 taagtccgct gtgaaaactc aaggctcaac ctcgagatgt cggcggaaac tatctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007504             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007504
VERSION     KHUT01007504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           91 a           28 c           65 g           70 t
ORIGIN      
        1 tacgggagga gcgagcatta ttcgggatga ttaggcgtaa agagtttgta ggtggttttt
       61 taagttgaaa gaaacaaatt aaagctcaac tttatacctt ttttcaaaac tgataaacta
      121 gagtataaat agaggataat ggaatttcta ttggagtgat aaaatacgtt gataatagaa
      181 ggaagaccta tggcgaaggc aattatctgg gtatatactg acactgagaa acgaaagctt
      241 gggtagcgaa cggg
//
LOCUS       KHUT01007505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007505
VERSION     KHUT01007505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggtcttg
       61 taagccagat gtgaaatccc cgggcttaac ttgggaactg catttgggac tgcaaggctg
      121 gagtacggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ctagtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007506
VERSION     KHUT01007506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           86 g           50 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcagaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaacgg ctgggtatac tggatgtctt
      121 gagccactta ggggcagtcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaaaaca gactgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007507
VERSION     KHUT01007507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttata
       61 taggtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gatatgactg
      121 gagtacaaga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttgatactga cactgaagcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007508
VERSION     KHUT01007508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           84 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggtttga
       61 taagtctttt gtgaaagctc ctggcttaac tgggagaggt caaaggaaac tgtcagacta
      121 gagtttggta ggggatactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctat ggcgaaagca ggtatctggg ccacaactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007509
VERSION     KHUT01007509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggttgg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tatcaatctt
      121 gaatatcctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg gggtcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007510
VERSION     KHUT01007510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           51 c           85 g           44 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 caagtccgcc gtcaaatccc agggctcaac cctggacagg tggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttccgg tggagtggtg aaatgcattg agatcggaaa
      181 gaacaccaac gacgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagaaaat ggg
//
LOCUS       KHUT01007511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007511
VERSION     KHUT01007511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          103 g           44 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggcaagc
       61 taagtctgtg gtgaaatcgc ccggctcaac tgggcggggt ccatggaaac tagctagctg
      121 gaggacggta gaggtgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gctcactggg ccggtcctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007512
VERSION     KHUT01007512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatacc
       61 taagtgcctt gtgaaatccc atggcttaac catggaatgg ctgggcatac tgggtgtctt
      121 gagccactca ggggctaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtagctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007513
VERSION     KHUT01007513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctctt
       61 taagtcagtg gtgaaatcct acagcttaac tgtagaacag ccattgatac tgaagagctt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaactcctat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007514
VERSION     KHUT01007514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           82 g           56 t
ORIGIN      
        1 tacgtaggga gcaagcgtta tccggattta ttgggtgtaa agggtgcgta gacggaaaga
       61 caagttagtt gtgaaatacc tcggcttaac tgaggaactg caactaaaac tatctatctt
      121 gagtactgga ggggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gctttctgga cagtaactga cgttgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007515
VERSION     KHUT01007515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          105 g           37 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggcggttttt
       61 tgcgtaggcg gtgaaagccc ccggcttacc cgggggaggt ccgtctaaac ggggaaactc
      121 gagggcggta ggggctggcg gaacgcccgg agtagcggtg aaatgcgttg agatcgggca
      181 gaacaccagt ggcgaaggcg gccggctggg ccgtccctga cgctgaggcg cgaaagccag
      241 gggagcgaac gga
//
LOCUS       KHUT01007516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007516
VERSION     KHUT01007516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcgttc
       61 caagtcaggc gtgaaattcc cgggcttaac ctggggactg cgcttgagac tggggtgcta
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccatttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007517             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007517
VERSION     KHUT01007517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          104 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcgcgca ggcggtgtgt
       61 taagtctgtg gtgaaagacc ggggctcaac cccggagtgt ccgcagagac tggcagactg
      121 gagtatcgga gaggagtgcg gaatacctgg tgtagcggtg gaatgcgtag agatcaggtg
      181 gaacaccgat ggcgaaggca gcactctggc cgagttactg acgctgaggc gcgaaagctg
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007518
VERSION     KHUT01007518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 cacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcgggggag
       61 gaggtctgat gtgacagctc gtggctcaac tacgagaggc cattggaaac cgctcttctt
      121 gaatcaggcc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg ccctgattga cgctgaggga cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007519
VERSION     KHUT01007519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcaag
       61 tgagtgggat gtgaaatgcc tcggcttacc cgaggacctg catctcatac tgcttggctt
      121 gagtacggga gaggatgaag gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ttcatctggc ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007520
VERSION     KHUT01007520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           91 g           46 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtccagg gtgaaatctt tcggcttaac cggaaaatag ccttggatac tgatagtctc
      121 gagggaggca ggggccgttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aacggctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007521
VERSION     KHUT01007521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccggagaga
       61 caagtcagat gtgaaatccg cgggcttaac ccgcgaactg catttgaaac tgtttccctt
      121 gagtatcgga gaggtaatcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gattactgga cgacaactga cggtgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007522
VERSION     KHUT01007522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           76 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtcagg
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgcctgactt
      121 gaattactag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactact agttgattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007523
VERSION     KHUT01007523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           89 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttata
       61 taagttatct gtgaaatccc tgggctcaac ctgggcaggt cagataagac tgtatgacta
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007524
VERSION     KHUT01007524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          102 g           48 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 tgcgtcgtcg gtgaaaaccc gtggcttaac tgcgggcttg ccgacgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007525
VERSION     KHUT01007525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           97 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccaac
       61 taagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgagac tggatggctt
      121 gagtgctgga gagggtggcg gaattcccca agtagaggtg aaattcgtag agatggggag
      181 gaacaccgga ggcgaaggcg gccacctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007526
VERSION     KHUT01007526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcatgg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgctttgctt
      121 gagtgctgga gagggtcgcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gcgacctgga cagtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007527
VERSION     KHUT01007527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           80 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtat agggcgcgta ggcggacctt
       61 ttagtcggat gtgaaatcct agggctcaac cttagaactg catccgaaac tggaggtcta
      121 gagggtagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctatc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01007528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007528
VERSION     KHUT01007528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctgtg
       61 taagtcaaag gtgaaatccc tcagctcaac tgaggaactg cctttgaaac tgcacagctt
      121 gaggccggga gggggtggcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007529
VERSION     KHUT01007529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcta
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaattg cgcccgaaac tgtagtgctg
      121 gagggagaca ggggtgagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007530
VERSION     KHUT01007530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggcccat
       61 caagtcgaat gtgaaagccc agagctcaac tctggaagtg catttgatac tggtgggctt
      121 gaatcctgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccagt ggcgaaggcg accacctgga cggagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007531
VERSION     KHUT01007531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgac
       61 taagtcagac gtgaaatccc caggctcaac ctgggaactg cgtctgatac tggttggctt
      121 gaatttggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccaacattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007532
VERSION     KHUT01007532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           52 c           78 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggggcgca ggcggcaaag
       61 taagtgggat gtgaaatccc ggggctcaac cccggacccg cattccaaac tactttacta
      121 gaggataggc ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttattcctga cgctcatgcc cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007533
VERSION     KHUT01007533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctatc
       61 cacgtcgggt gtgaaagctc agagctcaac tctgagacgc cattcgatac gggatagcta
      121 gaggcagcta ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg gctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01007534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007534
VERSION     KHUT01007534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c           99 g           37 t
ORIGIN      
        1 gacgaaccgt gctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagtccggg gtgaaatccc ccagctcaac tggggaagtg cctcggatac tggcggcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007535
VERSION     KHUT01007535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcaggc
       61 caagtccggg gtgaaagccc ccggctcaac cggggaagtg ccttggaaac tggcttgctc
      121 gagggaggta ggggcagagg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg ctctgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007536
VERSION     KHUT01007536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           95 g           40 t
ORIGIN      
        1 aacgtaggag acaagcgtta tccggagtta ctgggcgtaa agggcgcgca ggtggtggtg
       61 taagtgtggc gtgaaagccc ctggctaaac taggggacga cgaaacagac tgcacaacta
      121 gaggctgata gaagcacgcg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggtgaaaacg gcgtgctggg tcagacctga cactgaggcg cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007537
VERSION     KHUT01007537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgta
       61 taagattgat gtgaaatccc tgggcttaac ctaggaactg catcgatgac tgtacgacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007538
VERSION     KHUT01007538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggctttg
       61 caagtcaggc gttaaagacc caggctcaac ctggggaatg cgcttgatac tgcagagctt
      121 gagatcggaa gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ccgattctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007539
VERSION     KHUT01007539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           83 g           56 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggaatta ctgggtgtaa agggtacgta gacggcatat
       61 taagtcagtt gttaaattcc ccagcttaac tgggggtaag cgattgaaac tgataagcta
      121 gaggatggga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggc ccatttctga cgttgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007540             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007540
VERSION     KHUT01007540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           96 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagtcgtgc gtgaaagctc ccaggctcaa ccgggagagg tcgtgcgaga ctgccagact
      121 agaagtagag agaggccagt agaattccgg gtgtagtggt gaaatgcgta gagatccgga
      181 ggaataccag aggcgaaggc ggctggctgg ctctaacttg acgctcagac gcgaaagcat
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007541             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007541
VERSION     KHUT01007541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          108 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggcggcccgg
       61 tcagtccgtg gtgaaagcgc ggggctcaac cctgcgtcgg ccgtggatac tgctgtggct
      121 ggagcactgt agaggcaggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggcctgctgg gcagtggctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007542
VERSION     KHUT01007542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           92 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttttg
       61 taagtcgggc gtgaaagccc cgggctcaac ctgggaattg cactcgagac tgcaaagcta
      121 gggtgcggaa gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctggt ccagcaccga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007543
VERSION     KHUT01007543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           55 c          105 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggtggtccgt
       61 taagtcctgt gtgaaatctc ccggctcacc cgggaggggg cgcgggatac tgggggactg
      121 gaggcaggca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gccggctggg cctgttctga cactgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007544             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007544
VERSION     KHUT01007544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          101 g           49 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctgt
       61 taagtgagag gtgaaaggct ggggctcaac cccatgcagt gcctctcata ctggcggtct
      121 tgagttcggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccgatactg acgctgaggc gcgaaagctg
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007545             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007545
VERSION     KHUT01007545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagtgcgta ggcggcgcga
       61 caagtgaggg gtgaaatcct gtcggctcaa ccgtagggtg gccattcata ctgtcgcgct
      121 cgagggaggt aggggccggc ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc ggccggctgg atctctactg acgctgaggc acgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01007546             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007546
VERSION     KHUT01007546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           93 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggtc gctgaaatac cccggctcaa ccggggaact ggcatcgaaa cgacccgtct
      121 cgaggggcat agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagctcctgg atgtcttctg acgctgagac gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01007547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007547
VERSION     KHUT01007547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           77 g           60 t
ORIGIN      
        1 tacgtaggtc ccaagcgtta tccggaatta ctgggcgtaa agagtctgta gtcggtctcg
       61 caagtctact ttcaaatacc ttggcttaac caaggaaagg gggtggatac tgcgagtctg
      121 gaagttttca ggggtgagta gaatttccgg tggagaggtg aaattcgttg atatcggaag
      181 gaatgccaaa agcgaaagca gctcactatg aatatcttga cgatgagaga cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007548
VERSION     KHUT01007548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           97 g           44 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcataa agggtgcgta ggcgggccac
       61 caagtctgcg gtgaaattct ccagcttaac tggagtagtg ccgcggatac tgggggccta
      121 gagggagata ggggcgtgcg gaactctggg tggagcggtg aaatgcgttg atatccagag
      181 gaactccggt ggcgaaggcg gcacgctgga tcttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007549
VERSION     KHUT01007549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           91 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggtttaa
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaattg cgttgaatac tgttagacta
      121 gagtcggaca gagggcggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatcggt ggcgaaggcg gccgcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007550             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007550
VERSION     KHUT01007550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           99 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgagca ggcggtcggg
       61 taagttgtgt gtgaaagcgc cctggctcaa ccgggcgagg acatgcaaaa ctgccggact
      121 ggaggtaggt agaggcgagt ggaattccgg gtgtagtggt gaaatgcgta gagatccgga
      181 ggaacaccag tggcgaaggc gactcgctgg gcctattctg acgctcagac gcgaaagcat
      241 ggggagcgaa cggg
//
LOCUS       KHUT01007551             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007551
VERSION     KHUT01007551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           31 c           69 g           67 t
ORIGIN      
        1 tacgggaagg gcaagcgtta tccggcgtga ctgggcgtaa agagtccgta gacggtaaag
       61 taagtttatt gttaaatttt aaaactcaat tttaagaaag caataaatac tgctttactt
      121 tgagtttaat gtagaaaagt agaattttat atggtagggg tgaaatccgt agatatataa
      181 aggaatgcca acagcgaagg caactttttg gcataaactg acgttgaggg acgaaagtgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01007552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007552
VERSION     KHUT01007552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           99 g           41 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agagcgtgta ggcgggctgg
       61 caagtctggt gtgaaagctc ccggctaaac tgggagagtg cattggagac tgccaatcta
      121 gaggctgtca gagggtcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acgacctggg gcagaactga cgctgagacg cgaaagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007553
VERSION     KHUT01007553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcgggccgt
       61 taagttgggg gtgaaatccc ggagctcaac tccggaactg cctccaaaac tagcggcctg
      121 gagcaaagta gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actcgctgga cttttgctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007554
VERSION     KHUT01007554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcggat gtgaaatccc cgggctcaac ccgggaactg catacgaaac tgccaggctt
      121 gagtgtcgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007555
VERSION     KHUT01007555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca ggcggcatgt
       61 taagtcaact gttaaaatct ccggcctaac tgggggtatg cggtagatac tggcaagcta
      121 gagtatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007556
VERSION     KHUT01007556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           81 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcagaatta ctgggcgtaa agagcgcgta ggtggttttt
       61 taagtcaggt gtgaaatccc taggctcaac ctaggaactg catttgaaac tggaaaacta
      121 gagtttcgta gagggaggta gaattctagg tgtagcggtg aaatgcgtag atatctggag
      181 gaatacccgt ggcgaaagcg gcctcctaaa cgaaaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007557
VERSION     KHUT01007557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           91 g           42 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgcgca ggtggtctgg
       61 taagtcgggt gtgaaatccc ggggctcaac ctcggaagtg cacacgatac tgccggactc
      121 gagtactgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagatactga cactgaggcg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01007558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007558
VERSION     KHUT01007558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          104 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggtggaaggt
       61 tcagcgtggc ctgaaagccc ccggctcaac cggggagggc ggctgcgaac tggccttctt
      121 gagggcgaga gaggagcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcgctctggc tcgcccctga cactgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007559
VERSION     KHUT01007559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctctgatac tggaagtctt
      121 gagtgtgaga gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggc tcataactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007560
VERSION     KHUT01007560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtctgg
       61 taagtcggat gtgaaatccc ggagcttaac ttcggaactg cattcgatac tgccggactg
      121 gagtactgga gaggagatcg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007561
VERSION     KHUT01007561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggtggttcgg
       61 taagtcaggt gtgaaagccc ggggctcaac cccgggattg cacttgaaac tatcggactt
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007562             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007562
VERSION     KHUT01007562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcggct gtgaaatctc cacgcttaac gtggaggggt cagtcgatac tgtcgtgact
      121 cgagtccggt aggggagaat ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggttctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01007563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007563
VERSION     KHUT01007563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          100 g           50 t
ORIGIN      
        1 tacgggaggg gcgagcgttg ttcggaatta ttgggcttaa agggcgtgta ggcggcctgg
       61 taagtacttt gtgaaatccc ccggctcaac cggggacgcg cttggtatac tgctgggctt
      121 gaggcaggta gaggtgcctg gaactctagg tggagcggtg aaatgcgttg atatctaggg
      181 gaacgccggt ggcgaaagcg gggcactggg cctgacctga cgctgagacg cgaaagcgtg
      241 gggatcaaac ggg
//
LOCUS       KHUT01007564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007564
VERSION     KHUT01007564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcgggttat
       61 taagttagtt gtgaaatccc tgggcttaac ctaggaactg caactaatac tgataatcta
      121 gagtgctgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007565
VERSION     KHUT01007565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggaatca ctgggcttaa agcgtacgta ggcggatctg
       61 caggcgtcgt gtgaaatccc tcggctcaac cggggaactg catggcgaac cgcaggtctt
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaaaaca gaccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007566
VERSION     KHUT01007566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           97 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttcgt
       61 taagttcgag gtgaaatctc ccggctcaac tgggaggggt cttcggatac tggcggactt
      121 gaggagtgca gaggtgagtg gaattctcgg tgtagtggtg aaatgcgtag atatcgagag
      181 gaacaccagc ggcgaaggcg gctcactggg caccacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007567
VERSION     KHUT01007567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatca ttgggcgtaa agggcgcgta ggcgatttga
       61 ttagtctcgt gtgaaagccc acagcctaac tgtggaagtg cacgagatac tgtcgaattt
      121 gagttcagga gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctgga ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007568
VERSION     KHUT01007568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c           88 g           46 t
ORIGIN      
        1 tacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggccctt
       61 caagtgcttt gtgaaatccc ccggctcaac cggggaactg cttggcatac tgtggggctt
      121 gaatcatcta ggggcaactg gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaacgccaac ggcgaaagca ggttgctgga gatgcattga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007569
VERSION     KHUT01007569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcact
       61 caagtcggtg gtgaaagcct ccggcctaac cggagaagag ctgtcgatac tgggtgcctc
      121 gaggggtgta ggggtctgtg gaacttcggg tggagcggtg aaatgcgtag agatccgaag
      181 gaacgccggt ggcgaaagcg acggactgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007570
VERSION     KHUT01007570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgccttgctc
      121 gaggactgga gaggagattg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaaggccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007571
VERSION     KHUT01007571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c          100 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccct
       61 gaggtcgggg gtgaaatacc acggctcaac cgtggaagtg cccccgatac cgagggtctc
      121 gaggggggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acacgctgga ccctctctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007572
VERSION     KHUT01007572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttga
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgtctggctc
      121 gagtccggga gggggtggcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctgga ccggatctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007573
VERSION     KHUT01007573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcaggta ggcggcctca
       61 tatgtccctt gtgaaagccc tcggctcaac cgaggaactg caggggaaac tgtggggctt
      121 gagtccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctaagctg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007574
VERSION     KHUT01007574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          103 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaagct
       61 taagcgtgcc ctgaaagccc ccggctcaac cggggagggt gggtgcgaac tgggcttctt
      121 gaggtcgata gaggagagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gctctctggg tcggacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007575
VERSION     KHUT01007575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaaatag ccttcgatac tggtggtctg
      121 gaggaaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007576
VERSION     KHUT01007576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcgcgg
       61 ccagtcgggg gtgaaagccc acagcttaac tgtggaagtg cctccgatac tgccgcgctc
      121 gagggaggta ggggcagagg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ctctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007577
VERSION     KHUT01007577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           84 g           53 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggatatt
       61 taagtcggat gtgaaatccc tgggcttaac ctgggaattg cacccgatac tgagtatata
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga ccaatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007578
VERSION     KHUT01007578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggc
       61 atagtcaggg gtgaaatctt tcggctcaac cggaaaaggg cctctgatac tagtcgactc
      121 gagcgagata ggggcaattg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aattgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007579
VERSION     KHUT01007579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           90 g           47 t
ORIGIN      
        1 tacgtaccgg acaaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggcctag
       61 caggtggggt gtgaaagccc acggcccaac cgtggaattg cgcttcaaac cgctaggctt
      121 gagggagaca ggggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacacccgt ggcgaaggcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007580
VERSION     KHUT01007580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           80 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgca ggcggactat
       61 taagtcagtg gtgaaagccc acagctcaac tgtggaattg ccattgatac tggtagtctt
      121 gagtgcgtat gaagttggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacaccgat tgcgaaggca gctgactaat acgtaactga cgctcaggga cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007581
VERSION     KHUT01007581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          101 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgcgt
       61 caagtcggct gtgaaagccc ggggctcaac tccggaggtg cagccgagac tgacgtgctc
      121 gagtccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007582
VERSION     KHUT01007582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtacgca ggcggctttg
       61 taagacaggt gtgaaatccc cgggctcaac ctgggaactg catttgtgac tgcaaagcta
      121 gagtctgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg atgagactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007583
VERSION     KHUT01007583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctc
       61 caagtcgggg gtgaaatcgt tcggcccaac cggacaaggg cttccgatac tggagacctc
      121 gagggaggta ggggtcgagg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007584
VERSION     KHUT01007584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccat
       61 taagtcagaa gtgaaatctc agggctcaac cctgaaattg ctcctgatac tggtggactt
      121 gaggtaggta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007585
VERSION     KHUT01007585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           48 c           79 g           49 t
ORIGIN      
        1 tacgaagggt gcgaacgtta ttcggaatca ttgggcgtaa agggtgcgta gacggcatag
       61 aaagttggct gttaaatccc ccggcctaac cggggaccag cagtcaaaac tcctaagcta
      121 gaggatagaa gaaagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gcttcttagt ctattcctga cgttgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007586
VERSION     KHUT01007586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           77 g           57 t
ORIGIN      
        1 ttcgaggaag gcaagcgtta tccggaatca ttgggcgtaa agcgtgcgca agcggttttg
       61 taagtgaggt gtaaaattcc aacgctcaac gttggactcg cgcttcatac tgcaagactt
      121 gagaatagta gaggcaaatg gaattcccgg tgtagtagtg aaatgcgtta atatcgggag
      181 gaacgcctaa agcgaaagca atttgctggg ctatttctga cgccgttgca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007587
VERSION     KHUT01007587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           52 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccaat
       61 taagtagggt gtgaaatccc tcggctcaac cgaggaattg cgctctaaac tggttggctc
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007588
VERSION     KHUT01007588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatca ctgggcataa agggcgcgta ggcggcgatg
       61 aaagtcaggt gtgaaagccc tcagcttaac tgaggaattg cgcctgaaac tacattgctt
      121 gagtgtagga ggggagagtg gaactcctag tggagcggtg aaatgcgtag agattaggag
      181 gaacgccggt ggcgaaagcg actctctggc ctattactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007589
VERSION     KHUT01007589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           92 g           44 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcttaa agcgcgtgca ggcggtctga
       61 taagtcggac gtgaaatctc ctggctcaac tgggagaggc cgttcgatac tgccagacta
      121 gagggcagga gagggaggtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacacctgt ggcgaaagcg gcctcctggt ctgcccctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007590
VERSION     KHUT01007590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           94 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggactga
       61 tcagtccggg gtgaaatctc acggctcaac tgtgagtggt ccctggatac tgtcagtctt
      121 gagatgtcta gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gctctctggg gaccatctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007591
VERSION     KHUT01007591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 tacagagagt gcaaacgttg ttcggaatta ctgggcttga agcgcacgta ggcggcatcg
       61 caagtgcggg gtgaaatccc acggcttacc cgtggaactg cctggcagac tgcgatgctc
      121 gagtgtggta ggggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccacaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007592
VERSION     KHUT01007592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           94 g           51 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggtggttggc
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggccggcta
      121 gagtgtggga gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacgcctgc ggtgtagacg gcttcctgga ccatgactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007593
VERSION     KHUT01007593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           97 g           42 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcctgg
       61 aaagtcggtt gtgaaagccc ggggctcaac cccgggactg catccgatac ttccaggctg
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01007594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007594
VERSION     KHUT01007594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           80 g           53 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgttccat
       61 taagtcctcg gtgaaatctt cgggctcaac ccgaaaactg tcgaggaaac tagtggaatt
      121 gagtatggca gaggcagacg gaattgaagg tgtagcggta aaatgcgtag atatcttcaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccatcactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007595
VERSION     KHUT01007595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtctggt gtgaaaactc agagcttaac tctgagcttg catcgggtac gggcagacta
      121 gagtgtgcta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg gcataactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01007596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007596
VERSION     KHUT01007596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgcgg
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cgctagaaac tgccgtgcta
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gcttactgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007597
VERSION     KHUT01007597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggagcgta ggcggtttat
       61 taagtgggat gtgaaatccc cgggcttaac ctgggaattg cattccaaac tgattaacta
      121 gagtgcagga gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggct cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007598
VERSION     KHUT01007598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           92 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggatttg
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaagtg ccatggatac tgcgagtctt
      121 gaggaaggta ggggcatctg gaactgccgg tggagcggtg aaatgcgttg atatcggcag
      181 gaactccggt ggcgaaggcg agatgctgga tcttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007599
VERSION     KHUT01007599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           87 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttaggc gtgaaatgcc gaagctcaac ttcggtactg cgcttgatac tgccttgctt
      121 gagtgctgga gaagaagctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg agcttctaga cagttactga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007600
VERSION     KHUT01007600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           87 g           59 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtttggt gttaaagaac ggggcctaac cccggtagtg cactgaatac tggcagactt
      121 gagtgcggta gaggtaagtg gaattccaag tgtagcggtg aaatgcatag atatttggaa
      181 gaacaccggt ggcgtaagcg acttactggg ccgtaactga cactgaggaa cgaaagccgg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01007601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007601
VERSION     KHUT01007601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           94 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttttc
       61 taagtccgtt gtgaaagccc tgggctcaac ctgggaatgg cagtggatac tggagagcta
      121 gagtacggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007602
VERSION     KHUT01007602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          105 g           39 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcag
       61 gaagtcgggg gtgaaattcc ggggctcaac tccggaatgg ctgccgatac tcctggtctg
      121 gggaccggta gaggccggtg gaattgctgg tgtagcggtg gaatgcgtag agatcagcaa
      181 gaacacccgt ggcgaaggcg gccggctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007603
VERSION     KHUT01007603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcggatttg
       61 taagtcagag gtgaaagccc acggcttaac cgtggaactg cctttgatac tgcaagtctt
      121 gagtcctgga ggggctggcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga ggtgtagacg gccagctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007604
VERSION     KHUT01007604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           84 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggatctg
       61 taagtcagag gtgaaatctc acagcttaac tgtgaaactg cctttgatac tgcagatctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccgtaactga cgctcaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007605
VERSION     KHUT01007605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          109 g           35 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtggtc
       61 tatgtgccgt gtgacaggcg ggggctcaac ccccggctcg cagggtagac agggcgactg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01007606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007606
VERSION     KHUT01007606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          107 g           37 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcggcg
       61 taagcgtgtg gtgaaagcct ggggctcaac cccgggtcgg ccatgcgaac tgtgccgctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007607
VERSION     KHUT01007607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcggacaat
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatttgtctt
      121 gaatattgtt gaggtgggcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaaa caatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007608
VERSION     KHUT01007608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatg
       61 taagtgtgat gtgaaagccc tgggcttaac ctaggaagcg catcgcaaac tgtgtagcta
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007609
VERSION     KHUT01007609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           89 g           45 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgtacgta ggcggcccgg
       61 taagtccgtt gtaaaagtca aaggctcaac ctttgaaagc cgatggatac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gctcgctggg acggtactga cgctaaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007610
VERSION     KHUT01007610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c          100 g           49 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggtgggagca
       61 taagtccgct gtgaaagccc gaggctcaac ctcggagggt cggtggatac tgtgcttctt
      121 gaaggtgcta ggggaagatg gaattaccag tgtagcggtg aaatgcgtag atattggtag
      181 gaacaccagt ggcgaaggcg gtcttctggg gcactcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007611
VERSION     KHUT01007611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggcatgc
       61 atagtcttct gtgaaagcta cgggcttcac ctgtaaagtg cagaggatac tggcaagcta
      121 gagtgtcgga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007612
VERSION     KHUT01007612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           76 g           62 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactat
       61 taagtcagtg gtgaaatcct gcagcctaac tgtagaattg ccattgatac tgatggtctt
      121 gagtacattt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007613
VERSION     KHUT01007613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           96 g           38 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggcggcccgc
       61 caagtcagat gtgaaagacc ggggctcaac cccggaacgg catccgatac tggcgggctc
      121 gagggtttga ggggagtcgg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccggt ggcgaaggcg cgactctgga aaactcctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007614
VERSION     KHUT01007614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaaatt
       61 caagtccggc ttgaaagccc ccggctcaac cggggagggt ggctggaaac tgggtttctt
      121 gagggcgaga gaggggtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcaccctggc tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007615
VERSION     KHUT01007615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           53 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcggag
       61 taagtcgggt gtgaaatccc tcagctcaac tgaggaattg cgcccgaaac tactttgcta
      121 gaggaagata ggggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgtaagcg gcgtactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007616
VERSION     KHUT01007616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtttag
       61 caagtcactt gtgaaatctt tgggcttaac tcaaagcggc caagtgatac tgctagacta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg gattgctggg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007617
VERSION     KHUT01007617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          105 g           35 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggaggcg
       61 gacgtgccgt gtgacagacc agtgctcacc actggagggc cacggtacac gacgtctctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggt cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01007618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007618
VERSION     KHUT01007618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          105 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgtgcgca ggcggcctgg
       61 tatgtgccga gtgacaaatc cgcgcttaac gcggaagggt ctcggtagac agcgaggctg
      121 gaggccttga gagggaagtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagg ggcgcaggcg gcttcctggc aaggacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007619
VERSION     KHUT01007619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtgtgc
       61 cacgtccgtc gtgaaagccc atggcttaac cgtgggtctg cggtggatac gggcaggcta
      121 gaggcaggta ggggagaacg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007620
VERSION     KHUT01007620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatagg
       61 taagtcaggg gtgaaatccc agagcccaac tctggaactg cctttgatac tgccgatctc
      121 gaggatgaca gaggcaagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg acttgctggg tcatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007621
VERSION     KHUT01007621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tggcggtctt
      121 gggccatcta ggggcgaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgat ggtgaaaaca ggtcgctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007622
VERSION     KHUT01007622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           65 c          112 g           32 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcccgc
       61 tgcgcccggc gtgaaagccc ccggctcaac cggggaacgg cgtcggggac gggcgggctt
      121 gagggcggca ggggttggtg gaatgtccgg tgtagtggtg aaatgcgtag agatcggacg
      181 gaacacccgt ggcgaaggcg gccaactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007623
VERSION     KHUT01007623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           44 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcataa agggtgcgta ggcgggccac
       61 caagtctgtg gtgaaattct ctggcttaac cagagtagtg ccgcggatac tgggggcctc
      121 gaggggggta ggggcatgcg gaactctggg tggagcggtg aaatgcgttg agatccagag
      181 gaactccggt ggcgaaggcg gcatgctgga ccttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007624
VERSION     KHUT01007624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          100 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcact
       61 caagtcggtg gtgaaatact ccggcctaac cggagaaggg ctgtcgatac tgggtgcctc
      121 gagtggtgca ggggtctgcg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcggactggg cacctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007625
VERSION     KHUT01007625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          102 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggtctga
       61 tgcgtcgtgt gtgaaagccc ccggctcaac tggggagggt catgcgatac ggtcagactg
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01007626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007626
VERSION     KHUT01007626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           86 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttat
       61 taagttgact gtgaaagccc cgggctcaac ctgggaaagt cagccaatac tgatagacta
      121 gagtaccgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gctacctgga cggatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007627
VERSION     KHUT01007627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           34 c           79 g           71 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctgt
       61 taagtcagtg tttaaatttt gcagctcaac tgtaggtatg gcattgatac tgatggactt
      121 gaatattata gaggtagctg gaatttatgg tgtagcggtg aaatgcatag ataccataag
      181 gaacaccgat agcggaggca ggttaccatg tgattattga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007628
VERSION     KHUT01007628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggttttg
       61 taagtcaggc gtgaaagccc cgggcttaac ctgggaaatg cgtttgagac tgcaaggcta
      121 gagtgtgtca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ataacactga cgctcgggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007629
VERSION     KHUT01007629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           86 g           53 t
ORIGIN      
        1 gacagaggat gcaagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggtggttttg
       61 tgtgtctggt gtgaaagccc ttggctcaac caaggaagtg cgctagaaac tgcaaaactt
      121 gagttcggga gaggatggta gaattctcag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccggt ggcgaaggcg accatctgga ccgatactga cactaaagtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007630
VERSION     KHUT01007630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgcc
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg cattcgatac tgggcgacta
      121 gagtatgaga gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctggc tcaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007631
VERSION     KHUT01007631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           91 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtccggg gtgaaatcct ccagcttaac tggagaactg cctcggatac tggaggtctc
      121 gaggatgata ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaggcg gcatgctgga tcatttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007632
VERSION     KHUT01007632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           96 g           38 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg cctcggatac tggccatctc
      121 gagggagata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007633
VERSION     KHUT01007633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           56 c           78 g           48 t
ORIGIN      
        1 tacagagacc tcgagcgttt tccggaatca ttgggcgtaa agcgtccgca agtggccaga
       61 caagtcgaac ttaaaatctc acggctcaac cgtgaatctg ggttcgatac tgtccggcta
      121 gagaattgga gaggctgatg gaactcccgg tgtagcagtg aaatgcgtta atatcgggaa
      181 gaacaccaaa tgcgaaggca gtcagctggc caatttctga cacgcaggga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007634
VERSION     KHUT01007634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggtggttttg
       61 tgtgtctggt gtgaaagccc ttggctcaac caaggaagtg cgccggaaac tgcagaactc
      121 gagtttggga gaggatggta gaattctcaa tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctgga ccaacactga cactgatgtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007635
VERSION     KHUT01007635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           97 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggtgtaa agggcgcgta ggcggcatga
       61 caagtgtgat gtgaaagccc cgggcttaac ctgggaagtg cattgcggac tgtcaagctg
      121 gagtacatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007636
VERSION     KHUT01007636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggcttgt
       61 caagtcaggg gtgaaatccg gcagcttaac tgtcgaagtg cctttgatac tgatgagcta
      121 gagggagata ggggtatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007637
VERSION     KHUT01007637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggggaaa
       61 caagtggtgt atgaaatcac ccggcttaac cgggtggggt tatgccagac tgtttttctt
      121 gaggacgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgtacctga cgctcataca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007638
VERSION     KHUT01007638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtcg
       61 caagtctgtg gtgaaatccg tcagcttaac tgacgaactg ccgtggatac tgcgactctc
      121 gaggggtgta ggggcatgtg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg acgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007639
VERSION     KHUT01007639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           92 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcgc
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tagcggactt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007640
VERSION     KHUT01007640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           93 g           44 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtcggat gtgaaaactc gaggctcaac ctcgagaggt catccgaaac catttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gctctctggt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007641
VERSION     KHUT01007641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtctggg gtgaaatcct tgagcttaac tcaagaagtg ccctagaaac ggtaagacta
      121 gagtcttgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caagcactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007642
VERSION     KHUT01007642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatca ttgggcgtaa agggcgcgta ggcgattcta
       61 taagtcgtgt gtgaaagccc acagcctaac tgtggaagtg catacgatac tgtagaattt
      121 gagttcagga gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagc ggcgaaggcg gctttctgga ctgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007643
VERSION     KHUT01007643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcat
       61 caagtccggg gtgaaagcct ccagcttaac tggagaagtg cctcggatac tggtgggctt
      121 gagggaggta ggggcaactg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agttgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007644
VERSION     KHUT01007644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggggta
       61 caagtggtgt atgaaatatc ccagcttaac tgggaagggt tatgccagac tgtacttctg
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007645
VERSION     KHUT01007645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           94 g           39 t
ORIGIN      
        1 gacagggggc tcaagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggcccgt
       61 caagcgtttt gtgaaagccc tcggctcaac cgaggaacag caggacgaac tgacgggctc
      121 gaggcaggta ggggccggaa gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggggaagccg tccggctggg cctgtcctga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007646
VERSION     KHUT01007646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           85 g           50 t
ORIGIN      
        1 gacgagcctg acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgtgg
       61 taagtcaggc gtgaaatacc tcagcttaac tgaggaactg cgtccgatac taccatgcta
      121 gagtgagaca gaggtgagtg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaggcg actcactggg tctcaactga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007647
VERSION     KHUT01007647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           98 g           48 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgg
       61 tcagtcgaag gtgaaatccc cgagctcaac ttgggaactg ccttcgatac tgccgggctt
      121 gagtccggga ggggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007648
VERSION     KHUT01007648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggtggctcgt
       61 taagtcggat gtgaaagccc taggctcaac ctaggaagtg cattcgaaac tggcgagctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007649
VERSION     KHUT01007649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctgt
       61 taagtcctgt gtgaaagccc tgggctcaac ccaggaagtg cgcaggatac tggcaggctt
      121 gagtgacgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgttaactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007650
VERSION     KHUT01007650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           86 g           53 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agcgcacgta ggcgggcttt
       61 caagtcggtt gtgaaatccc tcagcttaac tgaggaactg cgaccgatac tgcttgtctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggc tcgtatctga cgctgaggtg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007651
VERSION     KHUT01007651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggtttt
       61 taagtcagag gtgaaatccc aaggctcaac cttggaactg cccctgatac tattagcctt
      121 gaatattgat gaggtgggcg gaacgggtca tgtagcggtg aaatgcttag agatgacccg
      181 gaacaccaat tgcgaaggca gctcactggg caattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007652
VERSION     KHUT01007652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           95 g           47 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtccgc
       61 taagttagat gtgaaaactc tgggctcaac ccagagactg catctaatac tgacgggcta
      121 gagttctgga gggggtagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagagtctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007653
VERSION     KHUT01007653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggcctgc
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg catacgaaac tggtgggctt
      121 gaatgtcgga gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg accacctgga cgaacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007654
VERSION     KHUT01007654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           49 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttcgt
       61 tatgtcagat gtgaaagccc ttggctcaac tgaggaagtg catctgaaac tggcgagcta
      121 gagtatgtaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg acaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007655
VERSION     KHUT01007655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggtggccaag
       61 taggtcagct gtgaaaactt gaggctcaac ctcaagctgt cggttgaaac cacttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cactgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007656
VERSION     KHUT01007656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacagaggtg acaaacgttg tccggaatta ttgggcgtaa agggcgcgta ggtggtcgag
       61 taagtcaggt gtgaaagctc tgggcttaac ccaggaattg catctgatac tgctcggctt
      121 gagtacggta gaggagggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccctctgga ccgaaactga cactgaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007657
VERSION     KHUT01007657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcggtg
       61 ttagtcggat gtgaaatccc ttggcttaac caaggaagtg catccgatac tgcatcgctt
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007658
VERSION     KHUT01007658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           99 g           45 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcggga gtgaaagcgc tgggctcaac ccagcaatgg ctctcgagac tggcaggcta
      121 gaggatccta ggggatgctg gaattccagg tgtagcggtg aaatgcgtag atatctggga
      181 gaacacccgt ggcgaaggcg ggcatctggg ggattcctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007659
VERSION     KHUT01007659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           86 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcgggaatt
       61 taagtgagat gttaaatcgt gtggctcaac catatcggtg catctcatac tgggttccta
      121 gaggacggca gaggcaagtg gaattggtag tgtagcagtg aaatgcgtag atattaccag
      181 gaacaccaat ggcgaaggca gcttgctggg ccgcccctga cgctgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007660
VERSION     KHUT01007660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcgggg gtgaaatact tcagcttaac tggagaagtg ccttcgatac tggcaagcta
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007661
VERSION     KHUT01007661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta ttcggattta ctgggcgtaa agcgcgtgca ggcggttcgg
       61 aaagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaatac ttccggacta
      121 gaggatgaga gagggaggtg gaattccggg tgtagtggtg gaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctggc tcataactga cgctaagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007662
VERSION     KHUT01007662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           94 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaataa ctgggcgtaa agggcgcgta ggcggctttt
       61 taagtctgga gtgaaagtcc cgcttttaag gtgggaattg ctttggaaac tggagagctt
      121 gagtgcagaa gaggtaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg acttactggg ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007663
VERSION     KHUT01007663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           97 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttcgc
       61 taagtccttt gtgaaagccc cgggcttaac ctgggaacgg cagaggatac tggcgggcta
      121 gagtgtggta gagggtggcg gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gccacctgga ccaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007664
VERSION     KHUT01007664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ctcggaatca ctgggcttaa agggtgcgta ggcggccttg
       61 taagttgggt gtgaaatacc tcggctcaac cgaggaattg cgctcaaaac tgcaaggctt
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tctctactga cgctgaggca cgaaagctag
      241 gggagcgaag agg
//
LOCUS       KHUT01007665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007665
VERSION     KHUT01007665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgtgta ggcggctctt
       61 taagtcaggt gtgaaatcct gtggctcaac cacagaactg cacttgaaac tgaagggctt
      121 gagtgtggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctttctggc ccaccactga cgctgagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007666
VERSION     KHUT01007666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggctttc
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaattg cttggcatac tgggaagctt
      121 gagccatcta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007667
VERSION     KHUT01007667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          103 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggcggtt
       61 taagtcgggt gtgaaagccc ggggctcaac cccgggacgc cgcccgaaac tggatcgctt
      121 gaggccggaa gaggggagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccggtcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007668
VERSION     KHUT01007668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           48 t
ORIGIN      
        1 aacgtagggt gcaagcgtta tccggattta ctgggcgtaa agcgcacgca ggtggcttgg
       61 caagttggac gtgaaagctc ccggcttaac tgggagaggt cgttcgaaac tgcgaggctt
      121 gaggttggga gaggggtatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007669
VERSION     KHUT01007669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           89 g           55 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtgggcctc
       61 taagtcgggg gtgaaatccc gcagcttaac tgcggaattg ctttcgatac tggaggtctt
      121 gagtacggca gagggagatg gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaacaccagt agcgaaggcg gtctcctggt ccgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007670
VERSION     KHUT01007670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcccgg
       61 ttagtccgac atgaaatgta ccggttcaac cggggcactg tgtcggaaac tgctgggctc
      121 gaggtcgagg gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctttctatc tcgaatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007671
VERSION     KHUT01007671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           90 g           49 t
ORIGIN      
        1 tacggagggg gcaaacgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggttcga
       61 ttagtcttgc gtgaaatctc tcggcttacc cgagaaactg cgctggatac tgtcgagctc
      121 gaggatggga gaggcagatg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacactcat ggcgaaggca gtctgctggc ccacacctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007672
VERSION     KHUT01007672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           98 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcctat
       61 caagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tgaggggctt
      121 gaggcaatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaacaccagt ggcgaaagcg gcaccctggc attggcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007673
VERSION     KHUT01007673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           99 g           37 t
ORIGIN      
        1 aacgtaggag acaagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggatggc
       61 taagacaggt gtgaaagcct ctggctcaac cgggggaggc cacctgtgac tggtcgtcta
      121 gagggcggac gaggggtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcactctaga ccgcacctga cgctgagacg cgacagcacg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007674
VERSION     KHUT01007674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           99 g           42 t
ORIGIN      
        1 tacggaggtg gcgagcgttg ttcggaatga ctgggcgtaa agggagcgca ggcggctttt
       61 taagtccggc gtgaaagccc ggggctcaac cccggcgggt cgccggatac tggcaagctc
      121 gagtacggaa gaggagagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccgatactga cgctcaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007675
VERSION     KHUT01007675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           92 g           49 t
ORIGIN      
        1 gatggtgggg gggcaagtgt tcttcggaat gactgggcgt aaagggcacg taggcggtga
       61 atcgggttga aagtgaaagt cgccaaaaag tggcggaatg ctctcgaaac caattcactt
      121 gagtgagaca taggagagtg gaatttcgtg tgtaggggtg aaatccgtag atctacgaag
      181 gaacgccaaa agtgaaggca gctctctggg tccctaccga cgctggggtg cgaaagcatg
      241 gggagcgaat agg
//
LOCUS       KHUT01007676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007676
VERSION     KHUT01007676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggccaga
       61 ttagtcaggc gtgaaagccc tgggctcaac ctggggactg cgcttgatac ggtttggcta
      121 gaggacggaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccgtacctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007677
VERSION     KHUT01007677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggcttcg
       61 cgagtccgat gtgaaagccc ggggctcaac cccggaagtg cattggatac tacggagctg
      121 gaggctggaa gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccagttctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007678
VERSION     KHUT01007678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggtggtctgt
       61 tatgttggat gtgaaagccc ttggctcaac tgaggaagtg catccaaaac tggcaggcta
      121 gagtactgaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg cagatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007679
VERSION     KHUT01007679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           74 g           63 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaagctc tcagcctaac tgagaaattg ccattgatac tgatgatctt
      121 gagtacattt gaagtgggcg gaatgtgtca tgtagtggtg aaatacttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007680
VERSION     KHUT01007680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgtat
       61 taagttagat gtgaaatccc tgggcttaac ctaggaactg catctaatac tggtacgcta
      121 gagtgcggta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctatctgga ctgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007681
VERSION     KHUT01007681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c          101 g           35 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggcccga
       61 tgcgtctgat gtgaaatctc ctcgctcaac ggggagaggc cattggaaac ggacgggctg
      121 gagggcagta gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007682
VERSION     KHUT01007682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           43 c           73 g           58 t
ORIGIN      
        1 gacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta ggtggtttgt
       61 taagttggat gttaaatccc tgagctcaac tcaggattgg tatccaaaac tagcaagctg
      121 gagacaggta gaggtagatg gaactctgtg tgtaggggta atatccgtag atatacagaa
      181 gaacaccaaa agcgaaagca atctactgga cctgttctga cactcaagaa cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007683
VERSION     KHUT01007683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           54 t
ORIGIN      
        1 tacgaaggtc cctagcgttg ttcggaatta ttgggcgtaa agcgcaggta ggtggctttg
       61 caagtcaggt gtgaaagcct ggggcttaac cccagaagtg cacttgaaac tgcttagctt
      121 gagtacggga gaggtgagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctcactggc ccagtactga cactgagctg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01007684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007684
VERSION     KHUT01007684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgagcgta gtcggtattg
       61 agagtcatag gtgaaatccc agggcttaac cctggaactg cccgtgagac ctcagtacta
      121 gagtgtgaga ggggatagtg gaataccaag tgtagcggtg aaatgcgtag agatttggtg
      181 gaacaccggt ggcgaaggcg gctatctggc tcacaactga cgatcaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007685
VERSION     KHUT01007685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           93 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtttcg
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgcgaggcta
      121 gagtgtggga tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctctctgga ccactactga cgctgaaaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007686
VERSION     KHUT01007686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          104 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggtcggc
       61 caagtccggg gtgaaaggcg ggggctcaac ccccgagctg cgctggaaac tggtcgactt
      121 gagtgtcgga gaggggagtg gaattctcgg tgtagcggtg aaatgcgtag agatcgagag
      181 gaacacccgt ggcgaaggcg gctccctggc cgaacactga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007687
VERSION     KHUT01007687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           89 g           56 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttag
       61 taagtcagat gtgaaatccc ggggcttaac tccggaagtg catttgaaac tactaggcta
      121 gagtgcagta gaggttattg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gataactggg ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007688
VERSION     KHUT01007688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggattaa
       61 aaagttctct gtgaaacacc taagcttaac ttaggaggtg cagagaagac taggagtcta
      121 gagtgacgta gagggtagtg gaattttcgg tgtagcggtg aaatgcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gctacctgga cgtaaactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007689
VERSION     KHUT01007689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           60 c          113 g           35 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggagtta ttgggcgtaa agcgcgcgta ggcggcgtgg
       61 tgcgtcgcac gtgaaagccc gcggctcaac cgcggagggt cgtgcgagac ggccgcgctg
      121 gaggcaggga gaggctggtg gaattcccgg agtagtggtg aaatgcgtag agatcgggag
      181 gaacaccggt ggcgcaggcg gccagctggc cctgacctga cgctgaggcg cgacggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007690
VERSION     KHUT01007690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           65 c          102 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgtc
       61 cgagtccggg gtgaaagccc gcggcccaac cgcggaagtg cctcggatac cggacgcctc
      121 gagtggggta ggggcacgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cccctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007691
VERSION     KHUT01007691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           62 c          107 g           34 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgggt
       61 cacgcgtgcc gtgaaagccc ggggctcaac cccgggtcgg cggtgcgaac gggctcgctc
      121 gagcgtacga gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggc gtacggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007692
VERSION     KHUT01007692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           47 c          101 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggacggc
       61 tttgtcgggc gtgaaattcc agggcttaac cttggggctg cgttcgagac gggttgtctt
      121 gagtggggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt cctttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007693
VERSION     KHUT01007693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggga gtgaaaacct ggggcttaac cccgggcctg ctttcgatac gggcagacta
      121 gagttcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007694
VERSION     KHUT01007694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          108 g           38 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggttcag
       61 tacatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgagat ggctggactg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gctggctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007695
VERSION     KHUT01007695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          108 g           35 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgggc
       61 caagcgtggg gtgaaagtcc ggggctcaac cccggatcgg cctcgcgaac tggctcgctc
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007696
VERSION     KHUT01007696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          106 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggagctt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggagggc ggctgcgtac tggagttctt
      121 gagggcgata gaggggggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccccctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007697
VERSION     KHUT01007697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           81 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtacgca ggcggatggt
       61 taagtccaaa gtgaaaggta ggagctcaac tcctacactg cttgggatac tggccgtctt
      121 gagtagcgga gagggagatg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca atctcctgga cgcaaactga cgctcatgta cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01007698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007698
VERSION     KHUT01007698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtggaaggt
       61 caagtccggc ttgaaagccc ccggctcaac cggggagagt ggctggaaac tggtcttctt
      121 gagggcgaga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccga ggcgaaggcg gccctctggc tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007699
VERSION     KHUT01007699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtccgtc gtgaaaaccc ggggctcaac cctgggcttg cggtggatac tgtcgggcta
      121 gaggatggta gaggggagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctccctggg ccattcctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01007700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007700
VERSION     KHUT01007700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ttgggcttaa agggcgcgta ggcggccgct
       61 caagtcgggg gtgaaatctt tcagctcaac tggaaaatag cctccgatac tgggcggctg
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007701
VERSION     KHUT01007701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcgggctcg
       61 taagtcagtg gtgaaatctc atagcttaac tatgaaactg ccattgatac tacgggtctt
      121 gagtaaggta gaggtagctg gaataagtag tgtagcggtg aaatgcatag atattactta
      181 gaacaccaat tgcgaaggca ggttaccatt tcttaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007702
VERSION     KHUT01007702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           94 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttctg
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgcagggcta
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtaggcg gctttctgga ccacgactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007703
VERSION     KHUT01007703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           87 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtctgt
       61 taaattagaa gtgaaagccc tgggctcaac ccgggaattg cttttaagac tggcaggctt
      121 gaatccgtaa gagggtagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actacctggt acggcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007704
VERSION     KHUT01007704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          104 g           41 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggagatc
       61 caagcccggg gtgaaaggtt ggggctcaac cccaacaggg ccctggggac tggatttctg
      121 gaggcaggca gaggagcgtg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcgctctggg cctgtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007705
VERSION     KHUT01007705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          101 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggcgtgta ggcggcttcg
       61 tgtgtctggc gtgaaagccc cgggcttacc ccgggaagtg cgttggaaac tgcggagcta
      121 gagtcccgga gagggaggtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gcctcctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007706
VERSION     KHUT01007706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           96 g           52 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatta ttgggtgtaa agggtgtgta ggcgggatgg
       61 taagtcagag gtgaaaggca gcggctcaac cgttggaagg cctttgatac tgcggtcctt
      121 gagttccgga gaggccatta gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaatacctga agcgtaggcg aatggctgga cggtgactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007707
VERSION     KHUT01007707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           96 g           42 t
ORIGIN      
        1 tacaggggga gcgaacgtta ctcggaatca ctgggcataa agggcgcgta ggcggttctg
       61 taagtcggat gtgaaagacc ttggctcaac caaggaaagg catccgatac tgcggagctt
      121 gaggccaaga ggggcaagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttgctgga ttggttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007708
VERSION     KHUT01007708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtgata
       61 tgtgagtgat gtgaaagccc tgggcttaac ctaggaagcg catcgcagac tatgtcactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01007709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007709
VERSION     KHUT01007709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta ctcggattta ctaggcgtaa agcgcatgta ggtggttgga
       61 taagtccatg gtgaaatttc ccggcttaac tgggaagggt ccatggaaac tatccggctt
      121 gagtgtggca aggggagacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaagca gtctcctggg ccaatactga cactgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007710
VERSION     KHUT01007710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcgaaatta ctgggcgtaa agcgtctgta ggcgattcgt
       61 cacgtctggg gtaaaagcac ggagctcaac tccgtgtatg tcctggaaac gagcgaggta
      121 gagtcatcca gaggcacctg gaatgccatg tgtaggggta aaatccgttg atccatggta
      181 gaacgccaaa agcgaaggca gggtgctggg ggtgtactga cgctgagaga cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007711
VERSION     KHUT01007711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           81 g           57 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggaattt
       61 taagtcggat gtgaaatccc taggcttaac ctaggaattg cattcgatac tgggattcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007712
VERSION     KHUT01007712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           98 g           37 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggcttgg
       61 cgcgtggggt gtgaaagcgg acggcttaac tgtccgaggc catcccatac ggctaagctc
      121 gagccatgca gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg catgagctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007713
VERSION     KHUT01007713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          109 g           31 t
ORIGIN      
        1 gacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcgcgc
       61 caagtcgggc gtgaaagccc ggggctcaac cccggaggtg cgcccgagac tggagcgctg
      121 gaggccggga gaggggggtg gaatacccag tggagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gccccctggc ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007714
VERSION     KHUT01007714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggcatat
       61 caagtctggt gttaaagctc ggagctcaac tccgaatcgg cactggatac tggtaagcta
      121 gagtacagta gaggcaaggg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgtaagcg ccttgctggg ctgttactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01007715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007715
VERSION     KHUT01007715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           91 g           45 t
ORIGIN      
        1 tacagagggt gctaacgttg ttcggaatca ctgggcttaa agcgcacgta ggcggccacg
       61 taagtgcgat gtgaaagccc ccggcccaac cggggaactg catcgtagac tgcatggctt
      121 gagagcggta ggggagacta gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaggcg agtctctggt ccgcttctga cgctcaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007716
VERSION     KHUT01007716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgat
       61 ccagtccggg gtgaaatcct gcagctcaac tgtagaagtg ccttggatac tgatcggctc
      121 gagggaggta ggggtcattg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg aatgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007717
VERSION     KHUT01007717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          102 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggttcgt
       61 cgcgtcggga gtgaaattcc acggcttaac cgtgggcgtg ctttcgatac gggcggactg
      121 gaggtaggca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007718
VERSION     KHUT01007718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggttgga
       61 caagtctggc gtgaaatctc ctggcttaac taggagcggt cgctggaaac tgtcgagctt
      121 gaggcgatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc gtcgtactga cgcacatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007719
VERSION     KHUT01007719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcgggttct
       61 caagtcggtg gtgaaatctt tcggctcaac cggaaaatcg cctccgatac tgggaacctc
      121 gaggggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007720
VERSION     KHUT01007720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgagcgcg gatggtcttg
       61 taagtcagga gtgaaagccc tgggctcaac ccaggaagtg cttttgatac tgcgagactt
      121 gaatgtggga gagggtgcta gaatacctag tgtagtggtg aaatacgtag atattaggtg
      181 gaacaccggt ggggaagccg ggtgcctggc ccaacattga catttaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007721
VERSION     KHUT01007721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           83 g           52 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggcggctatg
       61 caagttggaa gtgaaatccc agggctcaac cttggaattg ctttcaaaac tgcatagcta
      121 gagtctcgga gaggttagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgtaagcg gctaactaga cgagtactga cgctcaagca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007722
VERSION     KHUT01007722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           86 g           56 t
ORIGIN      
        1 tacgagggat cctagcgttg ttcggaatca ttgggcgtaa agggtgtgta ggcggccatg
       61 taagtcagtt gtgaaagccc cgggctcaac ccgggaagtg cttctgatac tgcttggctt
      121 gagtattgga taggtgagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggc ggcgaaggcg gctcactggc catatactga cgctgaaaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007723
VERSION     KHUT01007723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttaggt gtgaaatgcc gaggctcaac ctcagcactg cacttgatac tgccccgctc
      121 gagtgctaga gaagaagctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg agcttctaga tagttactga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007724
VERSION     KHUT01007724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           78 g           57 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggccttg
       61 taagtcagtg gtgaaatctc tcggcttaac cgagaaattg ccattgatac tgcagggctt
      121 gagtacagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaaa ctgtaactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01007725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007725
VERSION     KHUT01007725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggttctc
       61 aaagtcggat gtgaaagccc cgggctcaac ccgggaagtg cacccgatac tggagagcta
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaacactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007726
VERSION     KHUT01007726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           84 g           53 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ctcggaatta ctgggcgtaa agaatgcgta ggcgggcttg
       61 taagttggga gtgaaagccc tgggctcaac ccaggaatgg ctttcaaaac tgcaagcctt
      121 gaatgtgaca atgagtaggg gaattcctgg tgtaggggta aaatctgtag atatcaggag
      181 gaagaccgga agcgaaagcg cttacttagg tcaatattga cgctgatgca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007727
VERSION     KHUT01007727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          106 g           38 t
ORIGIN      
        1 gacgtaggat ccgagcgttg tccggagtta ctgggcgtaa agcgagcgca ggcggttgtg
       61 tgcatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgagat ggcacgactg
      121 gaggctggga gaggtcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgactgga ccagacctga cgctaaggct cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007728
VERSION     KHUT01007728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agcgtgcgca ggcggttgtg
       61 aaagtcagaa gtgaaatccc agggctcaac cttggaattg cttttgaaac ttcacgacta
      121 gagtactgga ggggttggcg gaatttcgag tgtagcagtg aaatgcgtag atattcgaag
      181 gaacaccgat ggcgtaggca gccaactgga cagttactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007729
VERSION     KHUT01007729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           85 g           55 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaatt
       61 caagtcagtt gtgaaatctc cgagcttaac tcggaacggt caactgatac tgttttgcta
      121 gagtacagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctggg ctgatactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007730
VERSION     KHUT01007730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          106 g           36 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcgggtcgt
       61 taggtacctg gtgacatgct cacgctcaac gtgagccggt ctgggtaaac cggcaacctg
      121 gaggcaggga gagggacgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcgtcctggc cctggcctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007731
VERSION     KHUT01007731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           82 g           55 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggttgtg
       61 ttagtctttt gttaaattct tcggcttaac cgggggcatg cgaaagaaac ggcacaactt
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcactcctga cgctgagaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01007732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007732
VERSION     KHUT01007732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacagagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctatg
       61 taagtcaggg gtgaaatcct ggggctcaac cccagaactg cctctgatac tgtgtagcta
      121 gagttttgga gaggatagtg gaatccctag tgtagaggtg aaattcgtag atattagggg
      181 gaacaccagt ggcgaaggcg actatctggc caattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007733
VERSION     KHUT01007733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           97 g           48 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaaacct gcagctcaac tgtgggcttg cagtcgatac gggcaggcta
      121 gaattcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatattga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007734
VERSION     KHUT01007734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggatgtg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcatatctg
      121 gagggatgca gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgactggg catctactga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007735
VERSION     KHUT01007735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattca ctgggcgtaa agcgcgcgta ggcggatttt
       61 taagttgggg gtgaaatgcc ggagctcaac tccggaactg cctccaaaac tggaagtctt
      121 gaggtatgga gggggaattg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gattcctggc catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007736
VERSION     KHUT01007736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcctag
       61 catgtcgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccgaaac tactaagctt
      121 gagtaccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccacctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007737
VERSION     KHUT01007737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           98 g           49 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtcttg
       61 tgtgtctacc gtgaaagcac acggcttaac cgtgtagagt cggtagaaac tgcgagactt
      121 gaggttggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacacttgt ggcgaaggcg gccagctgga ccaaatctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007738
VERSION     KHUT01007738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcaggta ggttgtcttg
       61 taagtctgct gtgaaatccc cgagcttaac ttgggaagtg cggcagatac tgcaggacta
      121 gagtgctgga agggttcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga cagtaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007739
VERSION     KHUT01007739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 gacgaaccgg tcgaacgtta ctcggaatta ctgggcttaa agggtgcgta ggcggcatgg
       61 aaagttgggt gtgaaatacc tcggctcaac cgaggaactg cgcccaaaac taccatgctt
      121 gagaaaggca gaggtgagtg gaacttgggg tggagcggtg aaatgtgttg atatcctaag
      181 gaacaccggt ggcgaaggcg actcactggg cctttactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007740
VERSION     KHUT01007740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacgtaggtg gctagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggcctga
       61 taagtcagat gtgaaatccc gaggctcaac ctcggactgg catttgaaac tgtcgggcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007741
VERSION     KHUT01007741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgt
       61 cgcgtcaatc gtgaaaacct gtggctcaac tacaggcttg cggttgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007742
VERSION     KHUT01007742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           93 g           47 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccgtt
       61 caagtagggt gtgaaatgcc tcggctcaac cgaggcactg cgctctaaac tgtccggctg
      121 gagtgagata ggggcgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccggt ggcgaaagcg gcacgctggg tctcgactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007743
VERSION     KHUT01007743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           84 g           59 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttatt
       61 taagtctgat gttaaatatt gtggcctaac cacaaaactg cattggatac tggatgactt
      121 gagtgcggta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgtaagcg acttgctggc ccgtaactga cgctgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01007744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007744
VERSION     KHUT01007744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctttc
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tggaaagctt
      121 gaggccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007745
VERSION     KHUT01007745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcggatgta ggtggctttg
       61 taagtcagat gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgccgga gaggttacta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccgga ggcgaaggcg ggtaaccagc cggacactga cactcagatc cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007746
VERSION     KHUT01007746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           86 g           49 t
ORIGIN      
        1 tacagagacc tcgagcgtta atcggaatca ctgggcttaa agcgtacgta ggcggacctg
       61 taggcatctt gtgaaatacc tcggctcaac cgaggaactg cttggtgaac cacaggtctt
      121 gaggcaagta ggggctgtcg gaacgatagg tggagcggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaaaaca ggcagctggg cttgtactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007747
VERSION     KHUT01007747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           76 g           60 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtctta
       61 taagtcagtg gtgaaatctt gtcgcttaac gacaaacgtg ccattgatac tgtaggactt
      121 gagtacagat gccgttggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gctgacgaaa ctgtaactga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007748
VERSION     KHUT01007748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtgcgg
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgatac taccgtacta
      121 gagtcccgga gaggttggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gccaactgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007749
VERSION     KHUT01007749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaagcg
       61 taagtgtggc ctgaaagccc ccggctcaac cggggagggt ggctgcaaac tgcgcttctt
      121 gagggcgata gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007750
VERSION     KHUT01007750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           85 g           47 t
ORIGIN      
        1 tacgtaccgt ccaaacgtta ttcggaatca ctgggcttaa agagttcgta ggcggtgcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaacag cgctcaaaac tactggacta
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg tctctcctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007751
VERSION     KHUT01007751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggttggt
       61 agagtcggct gtgaaatctc tgggcccaac ccagaggggt cggccgagac ctatcgactt
      121 gagggtggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccttctggg ccatacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cga
//
LOCUS       KHUT01007752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007752
VERSION     KHUT01007752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           58 c          108 g           40 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggtggcgcgt
       61 taagtctggc gtgaaagccc ccggctcaac cggggagggt cgtcggagac tggcgtgctt
      121 gagggcggta ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctgga ccgaccctga cactgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007753
VERSION     KHUT01007753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           78 g           54 t
ORIGIN      
        1 tacgtaggtc gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta tgcggttttt
       61 taagtccgtg gttaaatccc agagctcaac tctggaatcg tcatggaaac tggaaaactt
      121 gaggtgtgca gaggcagatg gaatttccga agtaggggta aaatccgtag atctcggaag
      181 gaacaccaaa cgcgaaggca ttctgctggg cacaatctga cgctgaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007754
VERSION     KHUT01007754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatga ctgggcgtaa agggagcgca gccggcttga
       61 ttagtctgga gtgaaagccc ggggcttaac cccggagggt cttcggatac tgtcaagctc
      121 gagtacggaa gaggagagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccgatactga cggtcaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007755
VERSION     KHUT01007755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttgta
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catctcatac tgtacggcta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007756
VERSION     KHUT01007756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           85 g           53 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggtggtatat
       61 caagtctggt gttaaatacc gaagctcaac ttcggaacgg cactggatac tggtaaacta
      121 gagtgcagta gaggcaaggg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgtaagcg ccttgctgga ctgtaactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01007757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007757
VERSION     KHUT01007757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgca ggcggtttct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggagaactt
      121 gagtgcagaa gagaaaagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctttttggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007758
VERSION     KHUT01007758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgggta ggcggctttg
       61 tgtgtccctt gtgaaagccc tcggctcaac tgaggaattg caaaggagac tacagagctt
      121 gagtccggga gaggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggtactga cgctgaactg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007759
VERSION     KHUT01007759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggtggtttgc
       61 caagtctgac gttaaatccc gaagctcaac ttcggttcgg catcggaaac tggcaggcta
      121 gagtctggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ccttgctggg ccagtactga cactgaggaa cgaaagccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01007760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007760
VERSION     KHUT01007760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtagggt gtgaaatccc tcggcttaac cgaggaatgg cgctctaaac taccgtgctt
      121 gaggaagata gaggagagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gctctctgga tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007761
VERSION     KHUT01007761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggagtga ctgggcgtaa agggcgcgta ggcaggatgg
       61 taagaagatt gtgaaaaccc cgggctcaac ctgggacgtg cagttttgac tgctgttctt
      121 gaatgtctaa gaggttagtg gaattgcagg tgtaggagtg aaatccgtag atatctgcag
      181 gaacaccggc ggcgaaggcg gctaactggt ggagcattga cgctgaggcg cgaaagcacg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007762
VERSION     KHUT01007762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttat
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaagtg cctttgatac tgatgggctc
      121 gagggaggta ggggcatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007763
VERSION     KHUT01007763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           89 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ctgggcgtaa agagagcgta ggcgggatcg
       61 taagttagaa gtttaataca tgggcttaac ccgtggtcgg cttttaatac tgcggttctt
      121 gagtgtggga gagggcgatg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt agcgaaggcg gtcgcctggc ctaacactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007764
VERSION     KHUT01007764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggtgtaa agggcgtgta ggcgggactg
       61 caagtcagat gtgaaatacg gaggcttaac ctccgagctg catttgaaac tgcaattctt
      121 gagtgatgga gaggtaagcg gaattcccag tgtagcggta aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gcttactgga cattaactga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007765
VERSION     KHUT01007765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          105 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcag
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaggtg cgcttgggac tgttgtgctg
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007766
VERSION     KHUT01007766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           98 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tggcaagctt
      121 gagcgtagta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctactgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007767
VERSION     KHUT01007767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttga
       61 taagtttgat gtgaaatccc tgggcttaac ctgggaacgg cgttgaagac tgttgaactg
      121 gagtcggtaa gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg acaacctggt accagactga cactgagggg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007768
VERSION     KHUT01007768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggcttga
       61 caagtcggtt gtgaaagccc cgggctcaac ctgggaactg catccgagac tgtcttgcta
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccaatactga cactcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007769
VERSION     KHUT01007769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgag
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tgctcgtctg
      121 gaggaaggta ggggcaggtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acctgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007770
VERSION     KHUT01007770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgta ggcggtttct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tgggaaactt
      121 gagtgcagaa gaggaaagtg gaattccatg tgtagcggtg aaatgcgcag agatatggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cgctgatgtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007771
VERSION     KHUT01007771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacgaagggt gctagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggcatgt
       61 taagtcaact gttaaagacc tcagcctaac tggggatcgg cggtagatac tgatgtgcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgctgatgca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007772
VERSION     KHUT01007772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg catacgatac tggcaagcta
      121 gagtgcaata gaggggggta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gccccctgga ttgacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007773
VERSION     KHUT01007773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctatt
       61 taagtctggt gtgaaagccc gaggctcacc ctcggaagcg cactggatac tggatggctc
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007774
VERSION     KHUT01007774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           83 g           63 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcctt
       61 taagtcagtg ctgaaatact ctagcttaac tagaggggtg gcattgatac tggaggactt
      121 gaatgaagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactagg cttagattga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007775
VERSION     KHUT01007775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           47 c           73 g           56 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtgcgca ggcggtaaag
       61 taagtcagtg gtgaaatctc tcggctcaac cgagaaactg ccattgatac tgctatactt
      121 gaatatagtt gaagtaggcg gaatgtgaca tgtagcggtg aaatgcttag atatgtcaca
      181 gaacaccgat agcgaaggca gcttactaaa ctaacattga cgctcatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007776
VERSION     KHUT01007776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           42 t
ORIGIN      
        1 tacagagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccctc
       61 taagtcgaac gtgaaatccc cgggctcaac ccgggaactg cgtccgatac tgaagggctc
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007777
VERSION     KHUT01007777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           97 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 tgcgtcgtgc gtgaaaggcc tgggctcaac ccaggaatgg cgctcgatac ggcaaggcta
      121 gagtgcagga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacctgt ggcgaaggcg gccatctgga ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007778
VERSION     KHUT01007778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggcctcg
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaagtg cattggaaac tgcgagactt
      121 gagtacggga gagggcagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007779
VERSION     KHUT01007779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           94 g           39 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcagag gtgaaatccc ggggctcaac ctcggaactg cctccgatac tgccgtgcta
      121 gagtgctgaa gaggagtccg gaattcacgg tggagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagc ggcgaaggcg ggactctggg cagcaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007780
VERSION     KHUT01007780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           57 c          108 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agggctcgca ggtggcccgc
       61 ggcgtcgtgc gtgaaagacc tcggctcaac cggggggtgt cgtgcgaaac gggcgggctg
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cactcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01007781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007781
VERSION     KHUT01007781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           90 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcggat gtgaaagccc acggctcaac cgtggaagcc cacccgaaac ggcctcgctc
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacacctgc ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007782
VERSION     KHUT01007782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           88 g           55 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggttaag
       61 atagtccgtc gttaaatgtc tgggctcaac ccagataccg cgtcggatac ttcttggcta
      121 gagaggcata gggggaaccg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacgccagt ggcgaaagcg ggttcctgga tgccttctga cgctgaggca ctaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007783
VERSION     KHUT01007783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           91 g           52 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggatgag
       61 caagtccgga gtgaaatcct tcggcttaac cggagaagtg ctttggatac tgcttgtctt
      121 gagggaggta ggggcatgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgtgctggc cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007784
VERSION     KHUT01007784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           92 g           42 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgca ggcggctcgt
       61 taagtcgact gtgaaagccc ttggctcaac caaggaagtg cagtcgaaac tggcgagcta
      121 gagtcccgaa gagggtggcg gaatgcccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccagt ggcgaaggcg gccacctggg aggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007785
VERSION     KHUT01007785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          101 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctggg
       61 gtagtcaacg gtgaaagctc ggggctcaac cccgagtcgg ccgtcgatac tcctcggctc
      121 gagcttacta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg gtaaggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007786
VERSION     KHUT01007786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          103 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggggggg
       61 caagtcggac gtgaaaggct tcggctcaac cggagacggt cgttcgaaac tgcccttctt
      121 gagggtcgaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gctctctggt cggctactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007787
VERSION     KHUT01007787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtccta
       61 caagtgagat gtgaaatccc cgggcttaac ctgggaactg catcttaaac ggtagggctg
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007788
VERSION     KHUT01007788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           41 c           76 g           55 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtccaa
       61 taagtcaact gttaaatctt gaggctcaac ctcgaaatcg cagtcgaaac tattagacta
      121 gagtatagta ggggtaaaga gaatttccag tggagcggtg aaatgcgtag agattggaaa
      181 gaataccgat ggcgaaggca ctttactggg ctattactaa cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01007789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007789
VERSION     KHUT01007789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattta ctgggcgtaa agcgcgtgta ggcggctggg
       61 taagttgggt gtgaaatctc ctggctcaac tgggagagga cgctcaagac tgcccggctc
      121 gaggacggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga ccgttcctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007790
VERSION     KHUT01007790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           95 g           42 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggtactt
       61 caagtcgggt gtgaaagccc cgggctcaac ctgggaactg cactcgagac tgaagcacta
      121 gagttcggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007791
VERSION     KHUT01007791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           87 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta ttcgaaatta ttgggcgtaa agcgcgagta ggtggtgagg
       61 taagttccgt gtgaaagcct cgggctcaac ccgagaatgg cacggaaaac tgccttgcta
      121 gagtacaaga ggggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagc ggcgaaagcg gctctctggc ttgtcactga cactcaatcg cgaaagggtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007792
VERSION     KHUT01007792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           85 g           54 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgt
       61 taagtttgtt gttaaagacc ttggcttaac tgaggaaaag caacaaaaac tggcgagcta
      121 gagggtgata gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggct gcttcttggt tcactactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007793
VERSION     KHUT01007793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttttg
       61 taagtcgggt gtgaaatccc tgggctcaac ctgggaactg cattcgagac tgcattgcta
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg actgcctggc ccaatactga cactcaaatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007794
VERSION     KHUT01007794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtttcg
       61 tgtgtctgat gtgaaatccc cgggctcaac ccgggaagtg cattggaaac tgcgagacta
      121 gagtcccgga gaggaaggtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007795
VERSION     KHUT01007795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggactgg
       61 caagttgggt gtgaaatccc tcagcttaac tgaggacgtg cattcaaaac tgccagcctt
      121 gagtacgaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007796
VERSION     KHUT01007796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggttgtg
       61 caagtcgggt gtgaaatcct ggagcttaac tccagaatgg cattcgaaac tgcacggcta
      121 gaggtctgga gaggtgactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaagcg agtcactgga cagaacctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007797
VERSION     KHUT01007797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           84 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgtgg
       61 ttagtcagat gtgaaagctc agggctcaac cctgaaactg catttgatac tgccatgctg
      121 gagctctgat ggggaggctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacaccagt agcgaaagcg agcctctgga cagatgctga cactgaagca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007798
VERSION     KHUT01007798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtccga
       61 taagtcggtt gtgaaatccc taggcttaac ctaggaactg catccgaaac tatcagactc
      121 gagtatcgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007799
VERSION     KHUT01007799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggtggtaggg
       61 tgtgtcagat gtgaaagccc tgggctcaac ccaggaagtg catttgaaac tactctactt
      121 gagtatggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccaatactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007800
VERSION     KHUT01007800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgca ggcggcctgg
       61 caagtcgaag gtgaaatccc tcggctcaac cgaggaattg ccttcgatac tgccaggctc
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007801
VERSION     KHUT01007801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtttgtc gtgaaatccc cgggcttaac ctgggaattg cgatgaagac tgcaaggcta
      121 gaatctggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007802
VERSION     KHUT01007802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          109 g           36 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggttgcc
       61 cgcgtcgcac gtgaaagccc ccggctcaac cggggagggt cgtgcgagac ggggcaactg
      121 gaggcaggga gaggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctga ggcgaaggcg gccagctggt cctgacctga cgctgaggcg cgaaggctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007803
VERSION     KHUT01007803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatccg
       61 taagtacctt gtgaaatccc accgctcaac ggtggaactg ctcggtatac tgcgggtctt
      121 gagccagcta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgat ggtgaaaaca ggttgctggg gctgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007804
VERSION     KHUT01007804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagcgagat gtgaaagccc cgggcttaac ctgggaattg catttcgaac tggcaggcta
      121 gagtgtagaa gaggggtgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg acaccctggt ctaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007805
VERSION     KHUT01007805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 gacgaaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagttgggt gtgaaagccc ttggcttaac caaggaactg cgctcaaaac taccgtgctg
      121 gaggaagaca gaggtgagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007806
VERSION     KHUT01007806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtacca
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaaggg catcgcaaac tatggaactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007807
VERSION     KHUT01007807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           97 g           43 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggctcgg
       61 aaagtcggtc gtgaaatcct ggggctcaac cccggaactg cgtccgatac ttccgagctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007808
VERSION     KHUT01007808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           96 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggtgtaa agggtgcgta ggcggacttg
       61 taagtcagcg gtgaaagact gcggctcaac cgtagagggg ccgttgatac tgccggtctt
      121 gagttccgga gaggtcactg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccaga agcgtaggcg ggtggctgga cggaaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007809
VERSION     KHUT01007809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggccttt
       61 taagattgac gtgaaagctc ctggcttaac cgggagaggt cgttgaagac tggaaggctt
      121 gagacaatga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc attggactga cgctcatgtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007810
VERSION     KHUT01007810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           98 g           47 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggattta ctgggcgtaa agcgcatgta ggcggcggat
       61 caagtgaggc gtgaaaggcc tgggctcaac ctgggaacgg cgcttcagac tggtttgctc
      121 gagttcggga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggc ccgagactga cgctcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007811
VERSION     KHUT01007811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          101 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcgggaagg
       61 caagtcggac gtgaaaggct tcggctcaac cggagacggc cgttcgaaac tgcttttctt
      121 gagggtcgga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gctctctggc cgacccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007812
VERSION     KHUT01007812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaatctc ggagctcaac tccgaaacgg catcggaaac tattcggcta
      121 gagggttgaa ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007813
VERSION     KHUT01007813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           95 g           49 t
ORIGIN      
        1 gacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttga
       61 taagtcgggc gtgaaatctc ctggctcaac tgggagaggc cgttcgatac tatcagactt
      121 gagggcagta gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcctcctgga ctgcccctga cactcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007814
VERSION     KHUT01007814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           96 g           48 t
ORIGIN      
        1 tacggaggtg gcgagcgttg ctcggattta ctgggtgtaa agggcacgta ggcggtctcg
       61 taagccctgc ttgaaattca ccggcttaac cggtggacgt ggtgggggac tgcaagactt
      121 gaggactgga gaggtgagcg gaattcccgg tgtaagggtg aaatctgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctcactggc cagtatctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007815
VERSION     KHUT01007815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggtccgta ggtggtccgg
       61 caagtctgat gtgaaatccc atggctcaac cgtggaactg cattggaaac tgccggactt
      121 gagtatcgca gaggtgaagg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg cttcactggg cgattactga cactgaggga cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007816
VERSION     KHUT01007816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcacgta ggcggataga
       61 tgtgtcatag gtgaaatccc cgggctcaac ctgggaactg cctgtgaaac tgtgtatcta
      121 gaatatagga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttcctggt ctaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007817
VERSION     KHUT01007817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           99 g           36 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtcgct
       61 taagtctgat gtgaaatcgc ccggctcaac cgggcgcagc cattggaaac tgggtgactg
      121 gagggcgaga gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggc tcgcccctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007818
VERSION     KHUT01007818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctttg
       61 caagtcggat gtgaaatccc tcggcttaac caaggaagtg catccgaaac tgcatggctt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007819
VERSION     KHUT01007819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcct
       61 caagtcaggg gtgaaatctt ccggctcaac cggaaaagtg cctctgatac tgaggacctc
      121 gagggaggta ggggcctgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcaggctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007820
VERSION     KHUT01007820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c          100 g           47 t
ORIGIN      
        1 tacgtaggtg tcgagcgtta tccggaatta ctgggcgtaa agggcacgca ggcggcctgg
       61 caagtccgac gtgaaagctc ccggcttaac tgggagaggt cgttggagac ttttgggctt
      121 gaaacggtga gagggaggcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gcctcctggc actgggttga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007821
VERSION     KHUT01007821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttcgc
       61 taagtcaggt gtgaaagccc ggggctcaac ctcggaactg catttgatac tggcgagcta
      121 gagtgtggta gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007822
VERSION     KHUT01007822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ctgggcgtaa agagagcgta ggcgggatcg
       61 taagttagaa gtttaatgca tgggcttaac ccatgacctg cttttaatac tgcggttctt
      121 gagtatggga gagggcgatg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt agcgaaggcg gtcgcctggc ccaatactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007823
VERSION     KHUT01007823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcaat
       61 caagtcgggg gtgaaatctt gcagcttaac tgtaaaagtg ccttcgatac tgattgactc
      121 gagggaggta ggggcaggtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007824
VERSION     KHUT01007824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           44 c           96 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatta ttgggtgtaa agggtgtgta ggcggggctg
       61 taagtcaggg gtgaaagtct gcggctcaac cgtaggattg cctttgatac tgcaggcctt
      121 gagtactgga gaggccactg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacacctga agcgtaggcg ggtggctgga cggttactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007825
VERSION     KHUT01007825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtctgt
       61 caagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tgatgggctt
      121 gaggcaatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc attggcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007826
VERSION     KHUT01007826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgtagggg ccgagcgtta tccggattta ctgggcgtaa agagcatgta ggcggtttgt
       61 taagtcggac gtgaaagctc ctggctcaac taggagaggc cgttcgaaac taacaaactt
      121 gagggtggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccattcctga cgctgagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01007827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007827
VERSION     KHUT01007827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttagg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgcctggcta
      121 gagtatggta gaggggggtg gaattcccgg agtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccccctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007828
VERSION     KHUT01007828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcaggt gtgaaatccc ggggctcaac ctcggaactg catttgaaac tggaagtctt
      121 gagaccagga gaggttagcg gaataccgag tgtagaggtg aaattcgtag atattcggtg
      181 gaacaccagt ggcgaaggcg gctaactgga ctggtactga cgctgaggtg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007829
VERSION     KHUT01007829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c          100 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgtccgca ggtggttttt
       61 taagactgac gtgaaagcgc ctggcttaac tgggcgaggt cgttggtgac tggaaagctg
      121 gaggcaagga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaataccaga ggcgaaagcg gcaccctggc cttggcctga cgctgaggga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007830
VERSION     KHUT01007830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtgtgt
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tggcacactt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007831
VERSION     KHUT01007831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          100 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggctaat
       61 caagtacgaa gtgaaatctc cctgctcaac ggggagggtg ctgcgtaaac tgaagagctt
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007832
VERSION     KHUT01007832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccaag
       61 taggtccgct gtgaaaactc gaggctcaac ctcgagccgt cggtggaaac catttggcta
      121 gagtccggaa gaggagagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007833
VERSION     KHUT01007833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           63 c          103 g           33 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggtggac
       61 caggtgccct gtgacatccc gcagctcaac tgcgggcggc cagggtagac cagtgcactg
      121 gaggcagaga gaggcaggtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgcaagcg gcctgctggc tctgaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01007834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007834
VERSION     KHUT01007834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcaggg gtgaaatccg tcggcttaac cggcgaagag cttttgatac tggtcgtctc
      121 gagggaggta ggggcatacg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gtgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007835
VERSION     KHUT01007835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcccg
       61 tcagtcaggg gtgaaatccc acggcccaac cgtggaagcg cctctgatac tgcgggcctc
      121 gaggggggta ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007836
VERSION     KHUT01007836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           99 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcggat
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggatac tgattcgctc
      121 gagggaggta ggggtcgcgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cgcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007837
VERSION     KHUT01007837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaacgt
       61 taagtccggt ctgaaagccc ccggctcaac cggggagggt ggctggaaac tggcgttctt
      121 gagggtgaga gaggtcggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gccgactggc tcatccctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007838
VERSION     KHUT01007838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           98 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggtcgcg
       61 ggcgtcggtg ctgacaattc gccgcttaac ggcgagaggc gtgccgatac gccgtgactt
      121 gaggccgtga ggggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa agcgaaagca acggactggc acggtactga cactgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007839
VERSION     KHUT01007839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgtt
       61 taagtttgat gtgaaagccc tgggcttaac ctaggaactg cattgaaaac tggacgactt
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggg cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007840
VERSION     KHUT01007840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          104 g           40 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtacgg
       61 caagtccggt gtgaaagcgc ccggctcaac tgggcgaggt cattggagac tgccgcactg
      121 gaggatggca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ccatacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007841
VERSION     KHUT01007841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          102 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggacggt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcgtac tggccgtctt
      121 gagggcgaca gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaggca gccctctggg tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007842
VERSION     KHUT01007842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           94 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcgggtcga
       61 cacgtcggaa ctgaaatccc ccggctcaac cggggaacgg gctccgatac gattgacctc
      121 gaggtgagaa gggggacctg gaacgtccgg tggagcggtg aaatgcgttg agatcggacg
      181 gaacgccgat ggcgaaagca aggtcctgga ctcattctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007843
VERSION     KHUT01007843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccgt
       61 cacgtcggct gtgaaaaccc ggggctcaac tccgggcctg cagtcgatac gggcggacta
      121 gagtacttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aagtaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007844
VERSION     KHUT01007844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           62 c          111 g           33 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtggcc
       61 taagcgcgcg gtgaaagcct ggggctcaac cccgggtcgg ccgtgcggac tgggcgactc
      121 gagcaccgta ggggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggc ggcgaaggcg gcctgctggg cggtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007845
VERSION     KHUT01007845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          102 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ctgggcgtaa agcgcgtgta ggcggcgcgt
       61 caagtcgggt gtgaaagccc ggggctcaac cccgggatgc cgctcgaaac tggcgcgctt
      121 gaggacggaa gaggggagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccgtccctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007846
VERSION     KHUT01007846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctcgg
       61 aaagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tcccgagctg
      121 gagtcccgga gaggaaagtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacatctgt ggcgaaggcg gctttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007847
VERSION     KHUT01007847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          101 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggggaag
       61 cgcgtcggac gtgaaaggcg ggagctcaac tcccgagcgg cgttcgaaac ggcttctctt
      121 gaggaccgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cggtatctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007848
VERSION     KHUT01007848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           60 c          107 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgca ggcggcccgt
       61 taagtcggcc gtgaaagctc cgggctcaac tcggagaggt cgggcgagac tggcgggctc
      121 gaggctggca gagggcggtg gaattccggg tgtagtggtg gaatgcgtag agctccggag
      181 gaacaccagt ggcgaaggcg gccgcctggg ccagccctga cgctgagacg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01007849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007849
VERSION     KHUT01007849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtctaa agggtgcgta ggcggtccat
       61 taagtcagtg gtgaaagacg gcagctcaac tgtcgcagtg ccattgatac tggtggactt
      121 gaatatcgat gaggctggcg gaattggtgg tgtagcggtg aaatgcatag ataccaccag
      181 gaacaccgat tgcgaaggca gctggctaat cgacgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007850
VERSION     KHUT01007850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttaa
       61 tacgtttgat gtgaaagccc cgggcttaac ctgggaacgg cgttgaagac ggttagactt
      121 gagtcggaga gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg acaacctggc tccagactga cactgagggg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007851
VERSION     KHUT01007851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgctg
       61 caagtcggtg gtgaaagctc gtcgcttaac ggcgagtcgg ccatcgaaac tgcagggctc
      121 gagcactgta ggggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagt ggcgaaggcg gttagctggg cagttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007852
VERSION     KHUT01007852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           59 c          107 g           41 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgtacgca ggcggcggcg
       61 ttggcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac cgcgtcgctt
      121 gaggatagca ggggttggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccaactggg ctattcctga cgctgaggta cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007853
VERSION     KHUT01007853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtctcg
       61 cgagtcgggt gtgaaagccc ggggctcaac cccggacgtg cattcgatac tacgaggctg
      121 gagtactgta gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007854
VERSION     KHUT01007854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           90 g           48 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgagca ggcggcatac
       61 caagttggac gtgaaatttt agggcttaac cctaaagggt cgtccaaaac tggtaagcta
      121 gagtgcagga gaggaaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctttctggc ctgtaactga cgctgaggcg cgaaggctgg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007855
VERSION     KHUT01007855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           88 g           46 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatctg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgcggatctt
      121 gagccaccta ggggcaaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca ggttgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007856
VERSION     KHUT01007856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagtcaggg gtgaaatccc gcagctcaac tgcggaagtg cctttgatac tggcggcctc
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007857
VERSION     KHUT01007857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           97 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaataa ctgggcataa agggcgcgta ggcgggccac
       61 aaagtcaggg gtgaaatccc acggctcaac cgtggaagtg cctttgatac tggtggcctg
      121 gagggaggca ggggagtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgctctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007858
VERSION     KHUT01007858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           82 g           57 t
ORIGIN      
        1 tacggggggt gctaacgtta tccggattta ttgggcgtaa agcgcatgta ggtggtttga
       61 taagtcagcg attaaatacc aacgcctaac gttgggagtg ttactgatac tgtcagactt
      121 gaggaaaaca ggggtacgcg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca gcgtactggg ttttacctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01007859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007859
VERSION     KHUT01007859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggccgtt
       61 caagtctgct gtcaaaggct ggggcttaac tccagacagg cagtggaaac tgaacagcta
      121 gagtgtggta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaagcg ctctgctgga ccacaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01007860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007860
VERSION     KHUT01007860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           66 c          104 g           38 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggcgcgg
       61 ggcgtcggtg ctgaaatcgg gccgcttaac ggcccgcggc gtgccgatac gcccgcgctg
      121 gagggtgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt ggcgcaagcg gccacctagc ccattcctga cgctgatgcg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01007861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007861
VERSION     KHUT01007861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           39 c           90 g           59 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtttat
       61 taagtctggt gttaaagatt ggggctcaac cctgggacag cactggatac tggtaggctg
      121 gagtgtggta gaggtaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgtaagcg acttactggg ccataactga cgctgaggaa cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01007862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007862
VERSION     KHUT01007862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgagg
       61 taagttggat gtgaaatctc ggggctcaac cctgaaactg cattcaatac tgccttgctc
      121 gagtattgga gaggagatcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaaggccagt ggcgaaggcg gatctctgga caattactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007863
VERSION     KHUT01007863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           80 g           52 t
ORIGIN      
        1 tacgagtacc ccgagcgtta tccggattta ttgggcgtaa agcgtgcgca gacggcttgt
       61 cgcatctctt gttaaatccc aaggctcaac cttggggccg cgggagacat ggataagcta
      121 gagactggaa gaggtaagcg gaattgctgg tgtaggggtt aaatccgtta atatcagcag
      181 gaacaccaaa agcgaaggca gcttactaga acagttctga cgttcaggca cgaaagcgtg
      241 gggagcgaat ggg
//
LOCUS       KHUT01007864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007864
VERSION     KHUT01007864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggaaaat
       61 caagtcagat gtgaaatacc ggggctcaac ttcggtgctg catttgagac tgtttttcta
      121 gagggtagga ggagaaaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01007865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007865
VERSION     KHUT01007865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc agagcttaac tctgaaactg ccattgatac tatctgtctt
      121 gaattctctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctcactacg gagtcattga cgctgaggca cgaaagcgtg
      241 aggatcaaac agg
//
LOCUS       KHUT01007866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007866
VERSION     KHUT01007866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           95 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtttag
       61 taagtcagat gtgaaagccc agggcttaac cttggaagtg catctgaaac tgttgaacta
      121 gagtctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg accgcctgga cgcagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007867
VERSION     KHUT01007867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           79 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttt
       61 taagtcagtg gtgaaagttt gcggcttaac cgtaaaattg ccattgaaac tgaaaggctt
      121 gagtatggtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gctcactaaa ccataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007868
VERSION     KHUT01007868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggattta ctgggcgtaa agcgcgcgca ggcggtcccg
       61 taagtcggac gtgaaagccc agggctcaac tctggaagtg cgtctgatac tgcgagactg
      121 gagtactgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagatactga cgctgaggcg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01007869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007869
VERSION     KHUT01007869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           84 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgtta ttcggattaa ctgggcgtaa agagcgtgtc ggcggcattt
       61 taagtcaact gtaaaagggc tcggcttaac cgagtaaatg caggtgatac tggaaagcta
      121 gagtacgaga gagaaaggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccttttggc tcgtaactga cgcttaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007870
VERSION     KHUT01007870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           97 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctac
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaacgg ccgtggatac tggtggtctc
      121 gagggaggta ggggcaagcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg gcttgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007871
VERSION     KHUT01007871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           87 g           52 t
ORIGIN      
        1 tacgtatgga gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcatgg
       61 caagtcagat gtgaaagccc ggggctcaac cccggaactg catttgaaac tgttaagctt
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007872
VERSION     KHUT01007872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           83 g           52 t
ORIGIN      
        1 tacgaaggtg acaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcgggtttg
       61 taagtcgatt gtgaaaaccc tgggctcaac ccgggacctg cagttgaaac tacaagtctt
      121 gaatactcga gaggatggtg gaattccagg agtagaagtg aaattcgtag atatctggag
      181 gaacaccgga ggcgaaagcg gccatctggc gagttattga cgctaaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007873
VERSION     KHUT01007873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           77 g           59 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacttg
       61 taagtcagtg gtgaaagccc gatgcttaac atcggaactg ccattgatac tgcaagtctt
      121 gaatatgttt gaagtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ccattattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007874
VERSION     KHUT01007874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           77 g           63 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggttta
       61 taagtcagtg gtgaaagccc atcgctcaac gatggaacgg ccattgatac tgtagatctt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccgat tgcgaaggca ggttactact aatggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007875
VERSION     KHUT01007875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           95 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtaagg
       61 tatgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tatcgaacta
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007876
VERSION     KHUT01007876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggttcgg
       61 aaagtcggat gtgaaaaccc ggggctcaac cccgggcctg catccgatac tcccgagctg
      121 gaggcagaca ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg tctgtcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007877
VERSION     KHUT01007877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgct
       61 caagtccggg gtgaaatcct ccagctcaac tggagaactg cctcggatac tgggtgtctc
      121 gagtgtggta ggggtgcgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcgcactgga ccacaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007878
VERSION     KHUT01007878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgcg
       61 caagtcgatt gtgaaaactc ggggctcaac cccgagcctg cgttcgatac tgcacggcta
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007879
VERSION     KHUT01007879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtttag
       61 tcagtctcta ctgacagtgt ccggctcaac cgggcaagtg gtagggaaac tgctaaactt
      121 gagggcttca gagggatggg gaactccagg tggagtggtg aaatgcgtag atatctggaa
      181 gaacaccaat ggcgaaggca ccatcctggg aagcatctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007880
VERSION     KHUT01007880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacgaggggg gctagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggcgct
       61 taagtgaggt gtttaatccc ggggcccaac cccggtccag cacttcaaac tgggtgtctt
      121 gaatagagga gaggtcgatg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccaat tgcgaaggca gtcgactgga ctcatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007881
VERSION     KHUT01007881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggccgac
       61 taagtcgggg gtgaaatcca ctggctcaac cagtgaagtg ccttcgggac tggacggctt
      121 gagtatggca gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgtaggcg gcttcctggg ccaatactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007882
VERSION     KHUT01007882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccaga
       61 caggtccgtt gtgaaaactg aaggcttaac cttcagacgt cgatggaaac cgtctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggctaaggcg gctctctagt acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007883
VERSION     KHUT01007883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           87 g           52 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgca ggccgccggg
       61 caagcgtgtt gtgaaatgca gtcgctcaac gtctgcactg cagcgcgaac tgcccagctt
      121 gagtgcgcgc aacgttggcg gaattcgccg tgtagcggtg aaatgcttag atatggcgaa
      181 gaactccgat tgcgaaggca gctgacgggt gcgtaactga cgctcatgct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01007884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007884
VERSION     KHUT01007884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          103 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggactta ctgggcgtaa agcgcgcgca ggcggatggg
       61 tatgtgccgt gtgaaagcgc cgtccttacg atggcgaggt catggtagac agcccgtctg
      121 gaggctcgaa gaggtcggtg gaattccggg tggagcggtg aaatgcgtag atatccggag
      181 gaacaccagg ggcgaaagcg gccgactggt cgagtactga cgctgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01007885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007885
VERSION     KHUT01007885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           95 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggaataa ctgggcgtaa agcgcacgca ggcggttcgg
       61 tgcgtggggt gtgaaaactg gcggcttaac tgccagaggc cacctcatac ggccgaactc
      121 gagctctgca gaggtccatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg cagcagctga cgctgatgtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007886
VERSION     KHUT01007886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggaatca ttgggcgtaa agggtgcgta gacggtatgg
       61 aaagtctgtt gttaaagatc ctagcttaac tgggaagatg caatggaaac ttctgtgcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggtc aaatgcgtag atctcgggag
      181 gaacaccgat ggcgaaggca gcttcttggc tcattcctga cgttcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007887
VERSION     KHUT01007887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           95 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcctt
       61 caagtcaggg gtgaaatcct ccggcctaac cggagaacag cccctgatac tgagggcctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007888
VERSION     KHUT01007888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggagcgg
       61 aaagtcaggg gtgaaatccc atagctcaac tatggaagtg cctttgatac tgccgttctt
      121 gagggaggca ggggagcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgctctggt cctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007889
VERSION     KHUT01007889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttcg
       61 atagtcgggc gtgaaatgcc tgggctcaac ccgggaactg cgttcgaaac ttcgaggcta
      121 gagtgtggga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007890
VERSION     KHUT01007890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           62 c           99 g           36 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgca ggcggcccgg
       61 tgcgtcagca ctgaaagcgc tcggcttaac cgagcgaggc gtgctgatac gaccggactt
      121 gaggctctga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc agagacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007891
VERSION     KHUT01007891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           97 g           38 t
ORIGIN      
        1 gacagaggag gcaagcgttg tccggactta ctgggcgtaa agcgtccgca ggtggtcgtg
       61 caagtgccgt gtgaaagccg gcggcttaac tgccggaggc catagcagac tgctcgactc
      121 gagaccggga gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca gctgactggc ccggtactga cactcaggga cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007892
VERSION     KHUT01007892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggtgtaa agggtgtgta ggcgggactg
       61 caagtcagat gtgaaaatta cgggctcaac ccgtaacctg cgtctgaaac tgtggttctt
      121 gagagtggga gaggtagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atctactgga ccacaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007893
VERSION     KHUT01007893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcttgg
       61 caagtccgct gtgaaaactc gaggcttaac ctcgagacgt cggtggaaac tgcttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007894
VERSION     KHUT01007894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccatc
       61 taagtctggt gtgaaatccc ggggcttaac cacggaagcg cactggatac tgggtagcta
      121 gagtactgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007895
VERSION     KHUT01007895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          101 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctccg
       61 ccagtcgggg gtgaaatccc accgcctaac ggtggaaggg cctccgatac tgcggggctg
      121 gagggaggta ggggcagcgg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg cgctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007896
VERSION     KHUT01007896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          103 g           37 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggcggatcgc
       61 taagtctgtc gtgaaagcgc ctggctcaac cgggcgaggt cgatggaaac tagcggacta
      121 gaggccggta gaggggggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgcgcctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007897
VERSION     KHUT01007897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           67 c          105 g           30 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 ccggtcggag gtgaaagccc ccagctcaac tggggaagcg cccccgatac cgccggcctg
      121 gaggggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gcacgctggg cccgccctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007898
VERSION     KHUT01007898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgtgta ggtggctcgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccgtgcgaac tggcgagctt
      121 gagtacagta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctgttactga cactgaaacg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007899
VERSION     KHUT01007899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           91 g           51 t
ORIGIN      
        1 gacgaactgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccat
       61 caagtcaggg gtgaaatcct gcagcttaac tgtagaagtg cctctgatac tgatggtctt
      121 gagggaggta ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007900
VERSION     KHUT01007900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           96 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggagtga ctgggcgtaa agcgcacgca ggtggttctg
       61 tgcgtggggt gtgaaagctc tcggctcaac tgagagaggc cacctcatac ggcagagctc
      121 gagccctgca gaggtccacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg caggcgctga cactcatgtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007901
VERSION     KHUT01007901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           62 c          111 g           36 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccatc
       61 taagcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac tgggtggctt
      121 gaggacggca ggggctggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgttcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007902
VERSION     KHUT01007902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           96 g           44 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcc
       61 caagtctgtg gtgaaatcct ccagctcaac tggagaactg ccgcggatac tgggggtctc
      121 gaggacggta ggggcgtgcg gaacagtagg tggagcggtg aaatgcgttg atatctactg
      181 gaactccggt ggcgaaggcg gcacgctgga ccgtttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007903
VERSION     KHUT01007903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           82 g           54 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtgatt
       61 caagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgaaac tagatcgcta
      121 gaattatgga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactgga catatattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007904
VERSION     KHUT01007904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           93 g           43 t
ORIGIN      
        1 tacgaaaggt gcgagcgtta ctcggaatca ctgggcgtaa agcgcgcgta ggtggacctg
       61 taggtcggtt gtgaaatacc tgggctcaac ccaggagggg cagccgaaac cacaagtctt
      121 gaatacctga gagggtgggg gaatacctgg tggagaagtg aaattcgtag agatcaggtg
      181 gaacaccgga ggcgaaggcg cctgcctggc agtgtattga cgctgaagtg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01007905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007905
VERSION     KHUT01007905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggattgc
       61 taagtcgggc gtgaaagccc caggctcaac ctgggaactg cgctcgaaac tggcagtctc
      121 gagtatcgga gagggcggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccgcctgga cgattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007906
VERSION     KHUT01007906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           81 g           55 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggaatca ttgggcgtaa agggtacgta ggcggcgaaa
       61 taagtcagtc attaaatctt ccagcttaac tggaagtctg tgactgaaac tgatttgctt
      121 gaggatggaa gagagaagcg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007907
VERSION     KHUT01007907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           86 g           56 t
ORIGIN      
        1 tacggagggt ccgagcgtta tccggaatta ttgggtttaa agggtccgca ggcgggcgat
       61 taagtcaggg gtgaaatggt gcggctcaac cgcagcactg cctttgatac tggttgtctt
      121 gagttatggt gaggtggccg gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaacaccgat cgcgaaggca ggtcaccaac catatactga cgctgatgga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007908
VERSION     KHUT01007908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggctcgt
       61 taagttgggt gtaaaagctc ctggctcaac tgggagaggt cgctcaagac tggcgggcta
      121 gagaatggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctggg ccatttctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007909
VERSION     KHUT01007909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           91 g           47 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgag
       61 caagtctatg gtgaaatcct ccggcttaac cggagaactg ccgtggatac tgcccatctc
      121 gaggaggata ggggcatctg gaactagcgg tggagcggtg aaatgcgttg atatcgctag
      181 gaactccggt ggcgaaggcg agatgctgga tcctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007910
VERSION     KHUT01007910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           89 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggttggta ggtggttacg
       61 tatgtctggg gtgaaatccc tgagctcaac tcaggaagtg ccttggaaac ggcgtaacta
      121 gagtgctaga gaggttcgtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga tagcaactga cactgaacaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007911
VERSION     KHUT01007911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagatc
       61 taagtgcttt gtgaaatccc ccggctcaac cggggaatgg cttggcatac tgggtctctt
      121 gagctgccta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggtgaaaaca ggttgctggg ggcatgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007912
VERSION     KHUT01007912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtttgt
       61 taagtcatgt gtgaaatccc tcggctcaac cgaggaaaga cgcatgaaac tggcaagcta
      121 gagtaccgga gaggggggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggt cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007913
VERSION     KHUT01007913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatca ctgggcgtaa agggcgtgca ggcggctcga
       61 taagtggaag gtgaaatgcc ctggctcaac cagggaactg ccttccatac tgtcgagctt
      121 gagtgcggga gaggatggga gaattcccgg tgtagcggtg gaatgcattg atatcgggag
      181 gaataccagt ggcgaaggcg cccatctggc ccgacactga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007914
VERSION     KHUT01007914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 gacgaaggtg gcgagcgttg ttcggaatca ctgggcataa agcgcacgta ggcggtgcca
       61 taagtcggat gtgaaatccc tcggcctaac cgaggaactg cgtccgatac tgtggtgctt
      121 gaggatcaga ggggtgcatg gaatttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggc ggcgaaagcg atgcactggc tgatttctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007915
VERSION     KHUT01007915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ctcggaatca ctgggcttaa agggtgtgta ggcggttcgg
       61 taagcatttt gtgaaagccc caggctcaac ctgggaactg caggatgtac tgccgggctt
      121 gaggcaagta gaggtcactg gaactctggg tggagcggtg aaatgcgtag atatccaggg
      181 gaacgcccat ggcgaaggca ggtgactggg cttgttctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01007916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007916
VERSION     KHUT01007916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatca ctgggcttaa agggcgcgca ggcggtcgga
       61 agggtgtcct gtgaaatccc tcggcttaac cgaggaactg cggggcaaac cgtccgactt
      121 gaggtaggta gaggttgatg gaactctggg tggagcggtg aaatgcgtag atatccaggg
      181 gaacgccaat ggcgaaagca gtcaactggg cctatcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007917
VERSION     KHUT01007917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           99 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgct
       61 caagtcgggg gtgaaagccc acagctcaac tgtggaagtg cctccgatac tgggcgcctg
      121 gagggaggta ggggtgcgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007918
VERSION     KHUT01007918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           83 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggatagg
       61 taagttagat gtgaaatccc cgggcttaac ctgggaactg cgtttaaaac tgccaatcta
      121 gagtaatgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007919
VERSION     KHUT01007919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggagtta ctgggcgtaa agcgcacgca ggcggtcgtg
       61 caagtttggg gttacagctc ccggctcaac tgggagagcg cctcgaagac tgcgcgactt
      121 gagggccaca gagggacaca gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggg tggcacctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007920
VERSION     KHUT01007920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggttgtg
       61 taagtcagat gtgaaatccc gaggctcaac ttcggaactg catctgatac tgcatgactc
      121 gagtgcagca gagggaaacg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gtttcctggg ctgacactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007921
VERSION     KHUT01007921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctct
       61 caagtcccac gtgaaatccc ccggctcaac tggggaatgg cgcgggaaac tgtgaggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007922
VERSION     KHUT01007922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           88 g           57 t
ORIGIN      
        1 gacataggtg gcaagcgtta ttcggaatta ctaggcgtaa agcgagtgta ggcgggtgct
       61 taagtctgtt gtgaaatctc ctggcttaac tgggaggggt caatggatac tgggcatctt
      121 gagtgaggta gggggtactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtacctggg cctttactga cgctaagact cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007923
VERSION     KHUT01007923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttgtg
       61 taagttggat gtgaaagccc cgggcttaac ctgggaactg cattcaaaac tgcacggcta
      121 gagtacggga gaggggggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccgat ggcgaaggca gccccctggc ctgatactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007924
VERSION     KHUT01007924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           89 g           43 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggcccgt
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaatgg catttgaaac tggcgagcta
      121 gagtgaatca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg aaatgactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007925
VERSION     KHUT01007925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttacg
       61 taagttaggt gtgaaagccc cgggcttaac ctgggaacgg cgcctaatac tgtgtagcta
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007926
VERSION     KHUT01007926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           89 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 caagtcagag gtgaaatccc ggagctcaac ttcggaattg cctttgatac tgttggtctc
      121 gagtccgaga gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007927
VERSION     KHUT01007927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           43 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggccgcg
       61 tatgtcccat gtgaaagccc tcggctcaac cggggaactg catgggaaac ggcgtggctt
      121 gagtccggga gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggtactga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007928
VERSION     KHUT01007928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           80 g           55 t
ORIGIN      
        1 tacagagggt gcaagcatta atcggattta ttgggcgtaa agagcgcgta ggcgggtttt
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac taagaatctt
      121 gagggtagtc ggagaaaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg attttctaga ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01007929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007929
VERSION     KHUT01007929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           89 g           52 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatcgt
       61 ttagtcaggg gtgaaatccc ggagctcaac ttcggaactg cctttgatac tggcgatctc
      121 gagtccgata gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactgga tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007930
VERSION     KHUT01007930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           94 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgta ggtggttcgt
       61 taagttggat gtgaaagccc cgggctcaac ctgggaatgg catccgatac tggcggactg
      121 gagttcgata gagggttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gcaacctgga tccgaactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007931
VERSION     KHUT01007931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           86 g           48 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc accgctcaac ggtggaaatg cttggcatac tggcgatctt
      121 gagccatcta ggggctactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtagctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007932
VERSION     KHUT01007932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           38 c           85 g           57 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggatata
       61 taagttagat gtgaaatacc tgggcttaac ctgggaggtg catttaaaac tgtatatcta
      121 gagtttgtca gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgtaggca gccccctggg ataagactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007933
VERSION     KHUT01007933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           77 g           54 t
ORIGIN      
        1 tacgaaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggttttg
       61 taagtcagtg gtgaaatctc ccggctcaac cgggaagctg ccattgatac tgcagaacta
      121 gagtacagat gacgttggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gctgacgaaa ctgcaactga cgctgatgca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01007934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007934
VERSION     KHUT01007934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           87 g           47 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgcgcgta ggtggtcttg
       61 tatgtcgatt gtgaaagccc agggctcaac cttggaatag cagccgaaac tgcaagactt
      121 gaatgttgga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctacctggc caaacattga cgctgaggcg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01007935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007935
VERSION     KHUT01007935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcttct
       61 taagtctgat gtgaaatctt gcggctcaac cgcaaatggt cattggaaac tgggaagctt
      121 gagtgcagaa gaggagagta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007936
VERSION     KHUT01007936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcatgg
       61 caagtcgggt gtgaaagccc tgggctcaac ctaggaagtg cactcgaaac tgccacgctt
      121 gagtcttgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga caatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007937
VERSION     KHUT01007937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggtggcttgc
       61 taagtcagtg gtgaaatcgt ccggcccaac cggacacgtg ccaatgatac tggtgagctt
      121 gagtgcgtga ggggtagggg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccaaa gccgaaggga ccctactggt acgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007938
VERSION     KHUT01007938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           80 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggccgcgcgg
       61 atagtcaaat gtgaaattca ggggctcaac ccctgcattg cgcttgatac ttccgtgcta
      121 gaactttgga gagggatctg gaattttcgg tgtagcagtg aaatgcgtgg atatcgaaaa
      181 gaacactaac ggcgtaggca agatcctgga caaatgttga cgctcatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01007939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007939
VERSION     KHUT01007939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           90 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggctttt
       61 taagtctatg gtgaaatctc ctggcttaac caggataggt ctgtagatac tggaaggctt
      121 gagtgtcgca gaggaaagtg gaattcccgg tggagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgtaagcg gctttctagg cgattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01007940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007940
VERSION     KHUT01007940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           83 g           56 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggggcca
       61 taagtcagtg gtgaaatctt gcggcttaac cgtaaaattg ccattgatac tgtggttctt
      121 gagtacgctt gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agtgaagaca gctcacgaaa gcgaaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01007941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU7999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007941
VERSION     KHUT01007941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU7999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 tcagtcggat gtgaaagccc cgggctcaac ctgggaatgg catccgatac tggcggactg
      121 gagttcgata gaggggtgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gcaccctgga tcagaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007942
VERSION     KHUT01007942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           85 g           57 t
ORIGIN      
        1 tacggggggt ccaagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggtggctttt
       61 caagtcaact gtgaaagccc agggcctaac cctggaagtg cagttgatac tgttgagctt
      121 gagtacgaga gaggatagtg gaatttctgg tgtagtggtg aaatacgtag atatcagaag
      181 gaacatcggt ggcgaaggcg gctatctggc tcgatactga cactgagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007943
VERSION     KHUT01007943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           89 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgagta ggttgttgat
       61 taagttatct gtgaaatgac tgggcttaac ctggtcctga gggatgatac tgattgacta
      121 gagtaaggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctattactga cactgagtca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007944
VERSION     KHUT01007944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           76 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggattta
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaactg ccattgatac tgtaagtctt
      121 gagtgtgttt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcactaag ccacaactga cgctgaggca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007945
VERSION     KHUT01007945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatca ttgggcgtaa agggtgcgta ggtggtgctg
       61 taagtctgac gtgaaatctc cgggcttaac tcggaatccg cgccaggaac tgcagtgctg
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccataactga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01007946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007946
VERSION     KHUT01007946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           99 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttgga
       61 caagttggtt gtgaaagcgc ccggctaaac tgggcgaggc cgatcaagac tgttcgacta
      121 gagggtgtta gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggggaagccg gcgccctggg acattactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007947
VERSION     KHUT01007947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           96 g           43 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtctgtc gtgaaagccc cgggctcaac ctgggaatgg cgatggaaac tggcgagcta
      121 gagtgcggta gagggcggtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccgcctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007948
VERSION     KHUT01007948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggctgt
       61 taagtcagtg gtgaaatctt caagcttaac ttggaaactg ccgttgatac tatcagtctt
      121 gaataacgtt gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaaa cgctcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007949
VERSION     KHUT01007949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           80 g           57 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattgt
       61 taagtcagtg gtgaaagctc ccggctcaac cgggaaattg ccattgatac tgacgatctt
      121 gagtgtggtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccacaactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007950
VERSION     KHUT01007950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgaggtg ccattgatac tgcggggctt
      121 gagtgttgat gaggcaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttgccaag caataactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01007951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007951
VERSION     KHUT01007951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 gacggaggag gctagcgttg ttcggaatta ctgggcgtaa agcgtacgca ggcggttgat
       61 caagtcaggt gtgaaagccc ggggctcaac ctcggaatag cgcctgagac tggtcagcta
      121 gagttcggaa gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg actctctggt ccgatactga cgctcaagta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007952
VERSION     KHUT01007952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           86 g           49 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtcagat gtgaaatccc ggggcttaac tccggaactg cgtctgaaac tacatggcta
      121 gagcattgga gagggtagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg actacctgga caattgctga cgctcaggca cgaaagcgtg
      241 ggtagccaaa ggg
//
LOCUS       KHUT01007953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007953
VERSION     KHUT01007953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           92 g           53 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggtcgat
       61 taagtctgac gtgaaatctc tcggcttaac cgggagcggt cgttggaaac taatcagctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcaccctggc attagcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007954
VERSION     KHUT01007954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           82 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggactat
       61 taagtcaggg gtgaaagccc gcagctcaac tgcggaactg cccttgatac tggtagtctt
      121 gagtataagt gaagttggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacaccgat tgcgaaggca gctggctaac ttataactga cgctgaggga cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007955
VERSION     KHUT01007955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           89 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcttcg
       61 caagtcggat gtgaaatacc taggctcaac caaggacgtg catccgaaac tgcgaggctt
      121 gagtacagaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007956
VERSION     KHUT01007956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           95 g           38 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggtggtcgcg
       61 caagtgccat gtgaaagccg gtggcttaac tgccggaggc catgacagac tgcgcaactg
      121 gagcccgtga gaggtccgtg gaattgccga tgtagcagtg aaatgcgtag atattggcag
      181 gaacaccgaa ggggaagcca gcggactggc acggtgctga cactgaagtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01007957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007957
VERSION     KHUT01007957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           89 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ttgggcgtaa agcgcacgca ggcggttctg
       61 cacgtggatg gtgacagctc ctggcttaac caggagagtg tcttccaaac ggcaggactt
      121 gagagcgaga gagggatacg gaactccagg tggagcagtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gtatcctggc tcgtttctga cgctcaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007958
VERSION     KHUT01007958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctgga
       61 aaagtttgat gtgaaagctt cgggcttaac ccggaaatgg cattgaaaac tgtctggctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007959
VERSION     KHUT01007959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           92 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggtggttctt
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tggagagctc
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctttctgga ccactactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007960
VERSION     KHUT01007960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           98 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgtacgca ggcggtcgcg
       61 ggcgtcggtg ctgaaaggcc accgcttaac ggtggtaggc gtgccgatac gccgcgactg
      121 gagccatgta gaggtccgtg gaattgccgg tgtagtggtg atatgcgtag atatcggcag
      181 gaacacccac ggggaagcca acggactggg catgtgctga cgctcaggta cgacagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01007961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007961
VERSION     KHUT01007961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           99 g           40 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggacggt
       61 caagtggtgt atgaaatcgc ccggcttaac cgggcggggt taggccagac tgaccgactg
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccagc ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007962
VERSION     KHUT01007962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagat gtgaaagccc cgggctcaac ctgggaactg catcttatac tgcgtagctg
      121 gagtgaagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cttacactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01007963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007963
VERSION     KHUT01007963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagatgggt gtgaaatccc tgggcttaac ctaggacggt cattcaagac tgaataactg
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007964
VERSION     KHUT01007964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggttgta
       61 caagtctgct gttaaagcgt ccagcttaac tggatacggg cagtggaaac tgtgcgacta
      121 gagtttggta ggggtcacgg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagc ggcgaaagcg cgtgactggg cctgaactga cactcatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01007965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007965
VERSION     KHUT01007965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggcggtctgt
       61 taagtcctgt gtgaaagccc ttggcttaac caaggaagtg cgcaggaaac tggcggactt
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007966
VERSION     KHUT01007966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           86 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaagca ggcggttcag
       61 taagtctttt gtgaaagctc ccggctcaac tgggagaggt caaaggaaac tactgggcta
      121 gagtgtggta gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccatcactga cgctgagttg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007967
VERSION     KHUT01007967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           99 g           39 t
ORIGIN      
        1 tacagagggg gcaagcgtta tccggattta ctgggcgtaa agggtctgta ggtggcggcg
       61 caggtggggt gtgacatctc ccggcttaac cgggagcggc cgctccaaac cgcgccactc
      121 gagccgtgca gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggcgcaagca gccgactggg cacgtgctga cactgaggga cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007968
VERSION     KHUT01007968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           88 g           56 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattta ttgggcgtaa agggtgcgta ggtggtgtgt
       61 taagtctgtt gttaaatttt ccggcctaac cggaagcatg caatggaaac tggcgcactt
      121 gaggatggaa gagggaagtg gaattctcgg agtagcggta aaatgcgtgg atctcgagag
      181 gaacaccgat ggcgaaggca gcttcctggt ccatatctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007969
VERSION     KHUT01007969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgca ggcggtttgt
       61 taagtctgat gtgaaagccc cgggcccaac ccgggaagtg cattggatac tggcagactt
      121 gagtacggga gaggagagcg gaattcctgg tgtaggggtg aaatccgcag atatcaggaa
      181 gaacaccggc ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007970
VERSION     KHUT01007970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcatcg gtgaaaactc caggctcaac ttggagactg ccggcgaaac tgccgtgctg
      121 gagtatggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007971
VERSION     KHUT01007971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcctta
       61 caagtcaggt gtgaaagccc tcggcttaac cgaggaagtg cgcctgaaac tgttcggctt
      121 gagtaccgga gagggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg accacctgga cggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007972
VERSION     KHUT01007972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           47 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agggtgcgca ggcggtctaa
       61 aaagttagac gtgaaatacc ccggcttaac cggggcaggt cgtctaaaac tattaaacta
      121 gagtgcagga gaggaaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctttctggc ctgcaactga cgctgaggca cgaaggctgg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007973
VERSION     KHUT01007973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc acggctcacc cgtggaactg ctgggcatac tggcggtctt
      121 gagccaccta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007974
VERSION     KHUT01007974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggttcgc
       61 taagttggat gtgaaatctt ccggctcaac tgggagagga cgtacaagac tggcggacta
      121 gaggacggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccggccctga cgctgagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007975
VERSION     KHUT01007975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           90 g           53 t
ORIGIN      
        1 tacagggggt gcaagcgtta ttcggaatta ttgggcgtaa agagcgtgta ggtggttcgt
       61 taagttcggt gtgaaagccc tgggctcaat ccaggaagtg cattgaatac tggcggacta
      121 gagtagagga gaggatggcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gccatctgga ctcttactga cactaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007976
VERSION     KHUT01007976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          102 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtccgct gtgaaagcct gcggcttaac cgcagagggt cggtggatac tgccagtctt
      121 gaaggtgcta gggagagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcttgga gcactcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007977
VERSION     KHUT01007977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgt
       61 taagtcagtt gtgaaagccc ggggctcaac cccggagggt cgactgatac tggtaagctg
      121 gagtacagaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg ggctactggg ctgatactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007978
VERSION     KHUT01007978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          100 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggattgt
       61 caagcctttg gtgaaaggct ggggctcaac cccaggagtg ccgaagggac tgacagtctg
      121 gaggcaggca gaggagagtg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacacccat ggcgaaggca gctctctggg cctgtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007979
VERSION     KHUT01007979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           89 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtgggtgtt
       61 taagtctggt ttgaaagcag gcggcttaac cgtctgatgt ggctggaaac tggacatctt
      121 gaatgggttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007980
VERSION     KHUT01007980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           95 g           47 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcagt
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaaggg ccttggatac tgactgtctt
      121 gagggaggta ggggtccgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaacgccggt ggcgaaagcg gcggactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007981
VERSION     KHUT01007981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          101 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ttgggcgtaa agcgtccgca ggcggtctgc
       61 tacgttcgga gtgacagtcg tcggcttaac tgacgaaggc cttggaagac gggcagactt
      121 gagggctcaa gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaat ggcgtaggca gtgtcctggg gagtacctga cgctgaggga cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01007982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007982
VERSION     KHUT01007982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           93 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcttga
       61 taagtcactc gtgaaatttc ccggctaaac tgggaagggt cgagtgatac tgttaggcta
      121 gagagcagca gaggatagta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gctatctggg ctgtgtctga cgctgaggcg cgaaggctag
      241 gggagcaaac agg
//
LOCUS       KHUT01007983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007983
VERSION     KHUT01007983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           96 g           47 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgtgta ggtggttatt
       61 caagttcggt gtgaaagcgc ccggctcaac tgggcgagga cattgaagac tgaatggcta
      121 gaggatggta gaggggcgta gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaataccagt ggtgcagacg gcgccctggg ccatacctga cactgagaca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01007984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007984
VERSION     KHUT01007984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggctgtgagg
       61 tcagtcggat gtgaaatccc ggggcttaac cccggaactg cattcgatac tgcctcacta
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttcctga cgctcatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01007985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007985
VERSION     KHUT01007985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggactat
       61 taagtcagcg gtgaaatccc agagcttaac tctggaactg ccattgatac tattagtctt
      121 gaatgttgtt gaggtgggcg gaacgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca gcccgctaag caactattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007986
VERSION     KHUT01007986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctgg
       61 taagtcggcg atgaaagccc ggggctcaac cccggaactg tcgtcgatac tgtcggactt
      121 gaatagagtg gaggctggcg gaatgggcgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctggctgta ctcttattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007987
VERSION     KHUT01007987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           92 g           45 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttact
       61 taagtcggat gtgaaagccc ttggctcaac caaggaagtg catacgaaac tgagtaactg
      121 gagtccgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007988
VERSION     KHUT01007988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           87 g           56 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggattag
       61 taagttggga gtgaaatccc ggggctcaac ctcggaattg ctttcaaaac tgctaatctt
      121 gagtgtagta ggggatgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccgat ggcgaaggca gtcatctggg ctacaactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007989
VERSION     KHUT01007989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggaagct
       61 taagtgtgat gttaaatcgt ggggctcaac cctgtcgatg catcgcatac tgggtttcta
      121 gaggacggca gaggcaagtg gaactcgtag tgtagcagtg aaatgcgtag atattacgaa
      181 gaacaccaat ggcgaaggca gcttgctggg ccgcccctga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01007990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007990
VERSION     KHUT01007990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctgg
       61 aaagtcaggt gtgaaatcct ggggctcaac cccagtcgtg cacttgaaac ttccaggctt
      121 gagtatggta gaggatgtgg gaatacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg cacatctggg ccataactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01007991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007991
VERSION     KHUT01007991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggccaag
       61 taagtctgac gtgaaatccc agggctcaac cctggaactg cgttggaaac tactcggctt
      121 gagtaccgga gaggtaaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ccttactggc cggttactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01007992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007992
VERSION     KHUT01007992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           39 c           96 g           54 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatga ttgggcgtaa agggcgcgta ggcggccatt
       61 taagaatgga gtgaaagtcc cgcttttaag gtgggaattg ctttgttgac tggatggctt
      121 gagtgtagga gaggttagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actaactgga ctataactga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007993
VERSION     KHUT01007993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           82 g           58 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggcggttttg
       61 taagttagat gtgaaagccc agggcttaac cctggaattg catttaagac tgcagaactt
      121 gaagtttgga gaggtaagca gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaataccaat ggcgtaggca gcttactggc caaaacttga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007994
VERSION     KHUT01007994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttga
       61 gaagtccgat gtgaaagccc cgggcttaac ctgggaacgg cgttggaaac tctcaagctt
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007995
VERSION     KHUT01007995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggcttgt
       61 taagtgaggg gtgaaatcct ggggctcaac tccagaactg cctttcatac tggcaagcta
      121 gagtccggaa gaggtgagtg gaactcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007996
VERSION     KHUT01007996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatcat
       61 taagtcaggg gtgaaatccc agggctcaac cctggaactg cctttgatac tggtgatctg
      121 gagttcggta gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01007997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007997
VERSION     KHUT01007997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           81 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccta
       61 taagtcagtg gtgaaatact gcagctcaac tgtaggattg ccattgatac tgtagggctt
      121 gagtacagat gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacacctat tgcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01007998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007998
VERSION     KHUT01007998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           48 c           77 g           52 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtatgca ggcggactaa
       61 caagtcagaa gtgaaatccc tgggcttaac ccaggaattg cttttgaaac tgtcagtcta
      121 gaatcccgga gaggttaaca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgcaagcg gttaactgga cgggcattga cgctcagata cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01007999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01007999
VERSION     KHUT01007999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           83 g           57 t
ORIGIN      
        1 tacgtaggac ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggatggg
       61 taagtcagag gtgaaagtct gcagcttaac tgtagaattg cctttgatac tgctcatctt
      121 gagtttggga gaggttgatg gaattccagg tgtagcagtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccataactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008000
VERSION     KHUT01008000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccga
       61 ttagtccgct ctgaaagtcc ggggctcaac cccgggaggc gggtggatac tgtcgggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008001
VERSION     KHUT01008001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggatgta
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaatgg catacgatac tgtacatctc
      121 gagttgaata gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcttcctgga ttcaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008002
VERSION     KHUT01008002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctatc
       61 caagtcagag gtgaaagccc tgggctcaac ccaggaattg cctttgaaac tggatagcta
      121 gagtgtcgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008003
VERSION     KHUT01008003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           89 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcctga
       61 taagtcagtg gtgaaaccct tgggcttaac ccaaggagtg ccatagatac tgttaggctt
      121 gggttacctc tgggcgggcg gaatggatgg tgtagcggtg aaatgcatag atatcaacca
      181 gaacgccgac agcgaaggca gctcgctggg ggtataccga cactgaggca cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008004
VERSION     KHUT01008004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggagcgta ggcggtttga
       61 caagttgggg gtgaaagccc ggggctcaac ctcggaattg ccttcaagac tgtcagactt
      121 gagtttggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccaatactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008005
VERSION     KHUT01008005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttat
       61 taagtcagtg gtgaaaacct gcagctcaac tgtagaattg ccattgatac tgatagactt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacacctat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01008006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008006
VERSION     KHUT01008006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          100 g           50 t
ORIGIN      
        1 tacgtagggt cctagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtcggat gtgaaagccc ggggcttaac tccgggtctg cattcggtac gggcatgctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008007             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008007
VERSION     KHUT01008007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           80 g           58 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggcctgg
       61 caagttatta gtggtaaatc gaggctcaac ctcgtggaaa ctagtaatac tgtccggctt
      121 gagtgcagga gaggttaacg gaactctatg tggaggggta aaatccatag atatatagat
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacactgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01008008             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008008
VERSION     KHUT01008008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           38 c           76 g           60 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtggag
       61 caagttgtta agtcaatgtc aagctcaact tgacggaatc tagcaatact actttactct
      121 gagggcaaga gaggttaacg gaattcggtg tggagcggtg aaatgcattg atatatcgag
      181 gaacaccaaa aaaggcgaag gcagttaact attttgatcc tgacattgag gcatgaaagc
      241 tgggggagca aaaagg
//
LOCUS       KHUT01008009             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008009
VERSION     KHUT01008009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           40 c           79 g           55 t
ORIGIN      
        1 gacataggtc gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtaaag
       61 taagttatca gtgggaaatc gaggctcaac ttcgtggaag ctggtaatac tgctatacta
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgcgtag atatatggaa
      181 gaacatcaat aacggcgaag gcagttaacc attctgctac tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01008010             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008010
VERSION     KHUT01008010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          101 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggcctgg
       61 taagtctcac tgtgaaagcc cggggctcaa ccccgggagg ccaggggata ctgcaaggct
      121 agagacaagt agaggctggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 ggaatacctg aggcgaaggc ggccagctgg gcttgatctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008011             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008011
VERSION     KHUT01008011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           79 g           57 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggttg
       61 aaatagtctt gggtaaaagc cagaagctca acttctgcgt ttgcttaaga tacttttcga
      121 ctagaggggc aaagaggtgc ttggaacgaa tggtgtagta gtgaaatgcg ttgatatcat
      181 tcggaacacc gaaagcgaag gcagagcact gggtgccacc tgacactgct aggacgaaag
      241 cgtgggtagc gaatggg
//
LOCUS       KHUT01008012             259 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008012
VERSION     KHUT01008012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>259
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           37 c           78 g           65 t
ORIGIN      
        1 tacgaggagg gcaagcgtta ttcggaatga ttgggcgtaa agggcatcta ggcggtgtgc
       61 attttttgtg atataaaatt ccagggctca accgtgggat tattacgaaa ttatatttgt
      121 acactagagt aaataaaagg aggatggaat ttccggagga ggggtaaaat ctgtagatat
      181 cggaaggaac atcgaaagcg taggcaattc tctagtattt tactgacgct gacgtgcgaa
      241 aacgtgggga gcaaccggg
//
LOCUS       KHUT01008013             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008013
VERSION     KHUT01008013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          102 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggccatc
       61 cgtaagtcgg ctgtgaaatc cccgggctta acccgggggg gtcggtcgat actgcggggc
      121 ttggaaggcg gtagggggag atggaattcc cggtgtagtg gtgaaatgcg tagatatcgg
      181 gaggaacacc gatggcgaaa gcgatctcct gggccgccct tgacgctgag gcgcgaaagc
      241 gtggggagcg aactga
//
LOCUS       KHUT01008014             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008014
VERSION     KHUT01008014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          100 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggccgca
       61 taagtctatg ggtgaaatgc cggggctcaa ccccgttgca tgcccaggat actgtgcggc
      121 tagagacacg tagaggcaag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacaccg gaggcgaagg cggcttgctg ggcgtgatct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01008015             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008015
VERSION     KHUT01008015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           99 g           50 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggttgga
       61 taagtctgtc tgtgaaagcc cggggctcaa ccccgtggat ggcaggggat actgtccgac
      121 ttgagacaag tagaggttgg tagaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aggaatacca gaggcgaagg cggccaactg ggcttgttct gacgctgagg tgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01008016             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008016
VERSION     KHUT01008016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           48 t
ORIGIN      
        1 tacgagggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccact
       61 acaagtcact tgtgaaatct ccgggcttaa ctcggtagcg gtcaagtgat actgttgggc
      121 tagagtgcgg aaggggcaac tggaattctc ggtgtagcgg tgaaatgcgt agatatcgag
      181 aggaacacct gcggcgaagg cgggttgctg ggccgacact gacgctgagg cgcgaaagct
      241 aggggagcga acggg
//
LOCUS       KHUT01008017             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008017
VERSION     KHUT01008017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           73 g           62 t
ORIGIN      
        1 taccagcagt tcaagtcgca tccctattta ttgggtctaa aacatccgta gcttgtgttt
       61 taagtctctt gtgaaatctc atgtctcaag catgaggctt gcgggagata ctgaaatgct
      121 agagatcggt agacgtaagg agtacgcgag aggtagcggt aaaatgtgtt aatctttcgt
      181 ggactaacaa tgagcgaagg caccttacga ggacgaatct gacagtgagg gatgaaggcc
      241 aggggcgcaa aacgg
//
LOCUS       KHUT01008018             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008018
VERSION     KHUT01008018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           34 c           77 g           68 t
ORIGIN      
        1 tacgagaggt gcaagcgttg tccggattta ttgggcgtaa agcgtttcta aaggcgcttt
       61 tgtaagttat cttttaaaga ccaaagctta actttgggaa gggagataat actgcaagag
      121 ttgaaatatt tcggggttac tagaactatc ggtgtagggg tgaaatccgt tgatatcgat
      181 aggaattcca agggcgaagg caggtaacta ggaatttttt gacgctgatg aacgaaagct
      241 aggggagcga aaggg
//
LOCUS       KHUT01008019             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008019
VERSION     KHUT01008019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccttt
       61 taagtctacc cgtgaaatgc cggggctcaa ctccgtgcac gccgggggat actgggaggc
      121 tggagacagg tagaggcaag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacacct gaggcgaagg cggcttgctg ggcctggtct gacgctgagg cgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01008020             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008020
VERSION     KHUT01008020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           42 c           80 g           56 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtcgaa
       61 taagttagtg gtgggaaatc gaggcctaac ctcgtggaaa ctattaatac tatccgactg
      121 gaggacagga gaggttgacg gaactctttg tgtaggggta aaatccgacg atataaagat
      181 gaacaccaat aacggcgaag gcagttaact attctgttcc tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01008021             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008021
VERSION     KHUT01008021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcgatg
       61 taagtcaggt gtgaaatctt ggggcttaac cccaacaagc attcgatact gcattgctag
      121 aggactggat gcgagattgg aattcacggt gtagcagtga aatgcgtaga tatcgtgagg
      181 aaggccagtg gcgaaagcga atctctggac agttcctgac gctgaggcac gaaggctagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01008022             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008022
VERSION     KHUT01008022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           73 g           64 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gacggtaaaa
       61 caagttacta cttgacgtcg agctcaactt gactgaaatt agtaatacta ttttgctaga
      121 gattgggaga agttgatgga aatctatgtg gaggggtgaa atccgttgat ctatagagta
      181 ccaccaaaag gcgaaggcaa ttgactattc catttctgac gttgaggcat gaaagcgtgg
      241 ggagcaaacc gg
//
LOCUS       KHUT01008023             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008023
VERSION     KHUT01008023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ttgggcgtaa agggagcgta ggcggccgcg
       61 taagtcaggt gttaaaattc gacgctcaac gtcgaagcgc atttgatact gcggggctcg
      121 aggagtggag aggagacagg aattcacggt gtagcagtga aatgcgtaga tatcgtgagg
      181 aagacccatg gcgaaagcat gtctctggaa actacctgac gctgatgctc gaaggccagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01008024             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008024
VERSION     KHUT01008024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           84 g           58 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agggtgagta ggcggtaata
       61 tgtgtcagat gtaaaaggct aaggctcaac catagttagc atttgaaact gtattacttg
      121 agtgcaggag aggtaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga cttactggac tgtaactgac gctgagtcac gaaagcgtgg
      241 gtagcaaaca gg
//
LOCUS       KHUT01008025             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008025
VERSION     KHUT01008025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggagcgca ggtgggaatg
       61 taagtcaatc ttaaaagtgc ggggctcaac cccgtgatgg gatagaaact acatttcttg
      121 agtgcaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctttctggac tgttactgac actgaggctc gaaagctagg
      241 ggagcgaacg gg
//
LOCUS       KHUT01008026             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008026
VERSION     KHUT01008026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           41 c           93 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggtgggatta
       61 taagtctttt gtaaaagtgc ggggctcaac cccgtgaagc ggaagaaact gtagttcttg
      121 agtgcaggag aggaaagcgg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtg gcgaaggcgg ctttctggac tgtgtctgac actgaggcgc gaaagctagg
      241 ggagcgaacg gg
//
LOCUS       KHUT01008027             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008027
VERSION     KHUT01008027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggtgggatca
       61 taagtcttat gtaaaagtgc ggggctcaac cccgtaaagc gtgagaaact gtgattcttg
      121 agtgcaggag aggaaagcgg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtg gcgaaggcgg ctttctggac tgtgtctgac actgaggcgc gaaagctagg
      241 ggagcgaacg gg
//
LOCUS       KHUT01008028             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008028
VERSION     KHUT01008028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ctgggcgtaa agggtgcgta ggcggccaat
       61 aaagtccggg gtgaaaggct acggctcaac cgtagtaagc cttggaaact tattggcttg
      121 agtgcaggag aggtaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga cttactggac tgtaactgac gctgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01008029             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008029
VERSION     KHUT01008029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           95 g           49 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggttctg
       61 cgtgtcggat gtgaaatctc actgaaaacg gtgaaactgc attcgaaact gcagggctgg
      121 agtgcaggag aggatggcgg aattctcggt gtagcggtga aatgcgttga tatcgagagg
      181 aacgccggtg gcgaaggcgg ccatctggac tgcaactgac gctgaggcac gaaagctagg
      241 ggagcaaaca gg
//
LOCUS       KHUT01008030             241 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008030
VERSION     KHUT01008030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>241
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           37 c           75 g           60 t
ORIGIN      
        1 tacggggggg gctagtgtta ctcgtaagaa ttgggcgtaa agggtccgca ggtggttgat
       61 aaaagttagt tgttaaattt tttaatgcaa cttatactaa tcaacttgag tttagtagag
      121 ggcaacggta ctcttagtgt aggggtaaaa tctgtggata ctaatgggga ctaccaaccg
      181 gcgaaggcgg ttgtctggcc taaactgacg ctaagggacg aaagcacggg gagcaaacag
      241 g
//
LOCUS       KHUT01008031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008031
VERSION     KHUT01008031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          107 g           40 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctcaa
       61 caggcctgac gtgaaagtcc tcggcttaac cggggagagt cgtcggggac cgttgagctt
      121 gagggcggca ggggctggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgactctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008032
VERSION     KHUT01008032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           93 g           44 t
ORIGIN      
        1 gacgaaccga gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggtctcg
       61 taagtctgcg gtgaaatcct ccagctcaac tggagaacgg ccgtggatac tgcgagtctc
      121 gagggaggta ggggcgtgcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008033
VERSION     KHUT01008033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcctgta ggcggattat
       61 taagttagct gtgaaaactc tgggcttaac ctagggcgtg cagctaagac tgatagtcta
      121 gagtaatgta gaggggggta gaattccagg tgtagcggtg gaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gccccctgga cattaactga cgctgagggg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008034
VERSION     KHUT01008034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtagcg
       61 tgtgagtgat gtgaaagccc cgggcttaac ctgggaagtg catcgctaac tgcgcaacta
      121 gagtacagca gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcggt ggcgaaggcg gccacctggg ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008035
VERSION     KHUT01008035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           98 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcac
       61 taagtctgat gtgaaagccc ccggcttaac cggggagggt cattggaaac tggtggactt
      121 gagtgcagga gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008036
VERSION     KHUT01008036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          101 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaacgt
       61 taagtccggt ttgaaagccc ccggctcaac cggggagagt ggctggaaac tggcgttctt
      121 gagggcggca gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg ccgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008037
VERSION     KHUT01008037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggctgg
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccattgatac tattagtctt
      121 gaatattgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccaat tgcgaaggca gctggctacg catatattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008038
VERSION     KHUT01008038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctgtg
       61 taagtttggt gtgaaagctc ggagcttaac tccgaaatgg cattgaatac tacatggctg
      121 gagggtcgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008039             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008039
VERSION     KHUT01008039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c          100 g           47 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcgggccgg
       61 taagtctgat gtgaaatctc catcgctcaa cgaggagagg tcattggata ctgccggcct
      121 ggagtacggt agaggaccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgttactg acgctgagga gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008040             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008040
VERSION     KHUT01008040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           49 t
ORIGIN      
        1 tacgaagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggcttgt
       61 taagtcgaat gtgaaagccc cgggcttaac ctgggaactg catccgatac tggcgagctt
      121 ggagtttgga agagggtggt ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 ggaacatcag tggcgaaggc ggccacctgg tccaaaactg acgctgatgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008041
VERSION     KHUT01008041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           59 c          109 g           36 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgta ggtggcgcgg
       61 tgcgtcggtg ctgaaagcgc cgggcttaac ccggcgaggt gtgccgatac gaccgggctc
      121 gaggccagca gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgccggt ggtgaagacg gccacctggg ctggacctga cactgaggcg cgatagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008042
VERSION     KHUT01008042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           90 g           56 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttttt
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggaaggctt
      121 gagtatggga gaggtatgcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcatactgga ccatcactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008043
VERSION     KHUT01008043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           97 g           41 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 caagtccgct gtaaaagtca acggctcaac tgttgaatgc cggtggaaac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat agcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008044
VERSION     KHUT01008044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           90 g           55 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggttttg
       61 taagtcttat gtgaaatcta tgggctcaac tcatagactg catgagaaac tgcagggctg
      121 gagtatggga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008045
VERSION     KHUT01008045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           84 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggtgtgg
       61 atagttgagt gtgaaatccc gaggcttaac ctcggaactg cactcaatac tcccatgcta
      121 gaggaatgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg catttcctga cgctcatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008046
VERSION     KHUT01008046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatga ctgggcgtaa agggtgcgta ggtggccgct
       61 caagttagta gtgaaattcc gaggcttaac ctcggcgcta ctactaagac tgagtggctt
      121 gagtgcagga gaggtaagcg gaattcctag tgtagcggtg gaatgcgtag atattaggag
      181 gaacaccggt ggcgaaagcg gcttactggc ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008047
VERSION     KHUT01008047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           90 g           57 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtctgat gtaaaagact ggggctcaac ctcgggactg cattggatac tggcagactt
      121 gagtgtggta gaggcaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgcaagcg acttgctggg ccataactga cactgaggaa cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01008048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008048
VERSION     KHUT01008048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           86 g           57 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcggtg
       61 taagttcggt gtgaaatttc agggctcaac cttgaaactg cattgaatac tgcatcgcta
      121 gaggattgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg caattcctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008049
VERSION     KHUT01008049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 gacgggggat gcaagcgtta tccggattga ttgggcgtaa agcgtctgta ggtggccaaa
       61 gaagtctacg gttccatact agggcttaac cctgggccag ccgtagaaac tctttggctt
      121 gagtgtggta ggggcagagg gaactcacgg tgtagcggtg aaatgcgtag atatcgtgag
      181 gaacaccgac ggcgaaggcg ctctgctggg ccgatactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01008050             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008050
VERSION     KHUT01008050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           84 g           54 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggcttta
       61 caagtcagat gtgaaatacc ggagctcaac ttcggtgctg catttgaaac tgtaaaggta
      121 agaggattgg cggagaaaat ggaattccac gtgtagcggt gaaatgcgta gatatgtgga
      181 agaacacctg tggcgaaagc ggttttctag ctaatacctg acgctgaggc gcgagagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008051             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008051
VERSION     KHUT01008051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          102 g           42 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggacgtg
       61 tgtgtctggc gtgaaatctc caccgctcaa cggggagagg ccgctggaaa ctgcgcgtct
      121 ggagtttggc agaggagcgt ggaattccgc atgtagcggt ggaatgcgta gatatgcgga
      181 agaacaccgg tggcgaaggc ggcgctctgg gccaaaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008052
VERSION     KHUT01008052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtctga
       61 caagtggaag gtgaaatccc cgcgcttaac gtgggaactg ccttccaaac tgtttgactt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008053
VERSION     KHUT01008053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           88 g           56 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtgggttcc
       61 taagtcggtg gtgaaatctt gcagctcaac tgcaaaattg ccgtcgatac tggggatctt
      121 gagtacggca gagggagatg gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaacaccggt agcgaaggcg gtctcctggt ccgtcactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008054
VERSION     KHUT01008054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           47 c           98 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtatgt
       61 cgcgtcgtga gtgaaaacca ctggcttaac tggtggcttg ctttcggtac gggcatactg
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008055
VERSION     KHUT01008055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcatgca ggcggtgcgt
       61 caagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaaaac tgacggactg
      121 gagcgtggta gaggaaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctggg ccacgactga cgctcagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008056
VERSION     KHUT01008056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           90 g           46 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgtc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg cttggtatac tgggcgtctt
      121 gagccactta ggggtcgtcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca gacgactggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008057
VERSION     KHUT01008057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcggga
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaactg cattgaatac tattctgctc
      121 gagggttgga agggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008058
VERSION     KHUT01008058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtctgg
       61 taagtccgtt gtgaaagtcc agggctcaac cctggaatgc cgatggatac tgtcaggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008059
VERSION     KHUT01008059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggctgtttcg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaattg cgcccgagac tgcggagctt
      121 gaatgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacattga cgctcatgta cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008060
VERSION     KHUT01008060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           84 g           53 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtctga
       61 ttagtctgtt gtgtaagctc ccggcttaac tgggagaggt caacggatac tgtcaggcta
      121 gagtatagga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaagca ggtaactgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008061
VERSION     KHUT01008061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           80 g           54 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatttg
       61 caagtcagaa gtgaaatccc agggcttaac cctggaattg cttttgaaac tgcaagtcta
      121 gaatcttgaa gaggttagca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccaat ggcgcaggca gctaactggg caagtattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008062
VERSION     KHUT01008062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctgga
       61 taagtcgatt gtgaaagcct tgggctcaac ctgagaattg cagtcgagac tgttcggcta
      121 gagtgtggga gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accacctggc ccaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008063
VERSION     KHUT01008063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcaat
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tggttgactt
      121 gagtactgga gagaagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actctttggc cagaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008064
VERSION     KHUT01008064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcttct
       61 taagtctgat gtgaaagccc gcagctcaac tgcggagggt cattggaaac tgggaagctt
      121 gagtgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008065
VERSION     KHUT01008065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           74 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggattat
       61 taagtcaggg gtgaaatctc cgagctcaac tcggaaactg cctttgatac tattagtctt
      121 gaatcctgtt gaggttgacg gaatataaca tgtagcggtg aaatgcatag atatgttata
      181 gaacaccgat tgcgaaggca gttaactaaa caggcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008066
VERSION     KHUT01008066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccacg
       61 caagtcggat gtgaaagccc caggcttaac ctgggaagtg cacccgaaac tgcatggctt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008067
VERSION     KHUT01008067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggcaggta ggctgtttta
       61 taagtctgac gtgaaatccc agggctcaac cctggaactg cgttggaaac tatatgactt
      121 gagtatcgga gaggttaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctaactggc cgattactga cgctgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008068
VERSION     KHUT01008068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           82 g           54 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggtggctatg
       61 taagttagaa gtgaaagccc agggctcaac cttggaattg cttttaaaac tacatagctt
      121 gaatgtcgga gaggatagcg gaatttccag tgtagcagtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgtaagcg gctatctgga cgacgattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008069
VERSION     KHUT01008069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtccggg gtgaaagccc ccagcttaac tggggaagtg ccttggatac tgatggtctt
      121 gaggggggta ggggcagtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cactgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008070
VERSION     KHUT01008070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgtaggaa gcaagcgttg cccggaatta ctgggcgtaa agagctcgta ggcgggggcg
       61 tgcgtccgaa aagaaatttc aaggctcaac cttggggcta tttcggatac ggcgcttctt
      121 gagggtgaga gaggaaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagc ggcgaaggcg gctttctggc tcattcctga cgctgaggag cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008071
VERSION     KHUT01008071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           40 c           90 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttatg
       61 taagttgact gtgaaagtcc tgggctcaac ctagggatgt cagttaagac tgtgtaactt
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg tctacctggc ctgatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008072
VERSION     KHUT01008072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           77 g           61 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggttgt
       61 taagtcagtg gtgaaagctc tcagcctaac tgagaaattg ccattgatac tgacgatctt
      121 gagtacattt gaggagggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctctctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008073
VERSION     KHUT01008073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           95 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcgggaact
       61 taagtctggt tttaaagacc ggggctcaac ctcggggatg gactggatac tgggtttcta
      121 gacctctgga gagagactcg gaatttctgg tgtagcggtg gaatgcgtag ataccagaag
      181 gaacaccgat ggcgaaggca gagtcttgga cagaaggtga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008074
VERSION     KHUT01008074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c           99 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgc
       61 caagtcagtg gtgaaatctc ccggcttaac tgggaggggc cctctgatac tggtaggctt
      121 gagggaggca gaggaaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg ccttacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008075
VERSION     KHUT01008075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtgggagta
       61 taagtgagct gtgaaatccc cgggcttaac ctgggcaagt cagcttagac tgtatttctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008076
VERSION     KHUT01008076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           91 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgtgagta ggcggtcacc
       61 gaagtcgggt gtgaaagccc agggctcaac cctggaagtg cacacgatac tgggtggctt
      121 gagttctgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagcaactga cgctgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008077
VERSION     KHUT01008077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           84 g           48 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggctgtttag
       61 caagttggaa gtgaaagccc agggctcaac cctggaattg ctttcaaaac tgcaaaacta
      121 gagtccggga gaggatagcg gaattcccag tgtagtagtg aaatacgtag atattgggag
      181 gaacaccagt ggcgcaagcg gctatctgga ccggtactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008078
VERSION     KHUT01008078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           95 g           40 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggcccg
       61 ttagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac tgcgggcctg
      121 gagggaaata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tttctactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008079
VERSION     KHUT01008079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           62 c           94 g           38 t
ORIGIN      
        1 gacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcccg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaaccg ctgggtatac tgcgggtctt
      121 gggccgacta ggggcagccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca ggctgctggg gtcgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008080
VERSION     KHUT01008080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           78 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggacggg
       61 taagtccgtg gtgaaatctc tgagcttaac tcagaaactg ccgtggatac taccggtctt
      121 gaataaggtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gctcactacc ccttcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008081
VERSION     KHUT01008081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           77 g           54 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggcccgg
       61 taagtcagtg gtgaaagcct gttgcttaac aacagaactg ccattgatac tgccaggctt
      121 gagtacagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01008082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008082
VERSION     KHUT01008082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           91 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggtctag
       61 caggtcggat gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac cgcaagactc
      121 gagtcccgga gagggtagtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaagcg gctacctgga cgaggactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008083
VERSION     KHUT01008083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           79 g           56 t
ORIGIN      
        1 tacgaagggg gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtttag
       61 taagttggaa gtgaaagcct agggctcaac cttagaattg ctttcaaaac tactagacta
      121 gagtactaaa gaggatagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccgga agcgaaagcg gctatctggt tagttactga cgctgttgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008084
VERSION     KHUT01008084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           88 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggttcgta ggttgttaat
       61 caagttatct gtgaaagccc tgggcttaac ctgggaaggt cagataaaac tggagaactc
      121 gagtattgga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc caaatactga cactgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008085
VERSION     KHUT01008085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           93 g           51 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagttgaat gtgaaaactc tgggcttaac ccagagcctg cattcaatac tggcaggctg
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagaaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008086
VERSION     KHUT01008086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           99 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggagtta ctgggcgtaa agggtccgca ggcggctacg
       61 caagtgtcgt gtgaaagccc cgtgcttaac acggggaggc cacggcagac tgcgcagctc
      121 gagtgatgca gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggcgaaagca gccacctggg catcaactga cgctgaggga cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008087
VERSION     KHUT01008087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctacg
       61 tgtgtcggac gtgaaatccc atggctcaac catggaagtg cgctcgaaac tgcgtagcta
      121 gagtcctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg accttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008088
VERSION     KHUT01008088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           83 g           53 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtgggtccg
       61 taagtacctt gtgaaatccc atggctcaac catggaactg ctgggtatac tgcgggtctt
      121 gagctatcta gaggttactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtaactggg gatatgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008089
VERSION     KHUT01008089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           55 c          106 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcttgg
       61 taagtcggcc gtgaaatctc caggctcaac ttggaggggt cgggcgagac tgccaggctc
      121 gaggtgggta gagggcagtg gaattccggg tgtagtggtg gaatgcgtag agctccggag
      181 gaacaccagt ggcgaaggcg gctgcctggg cctggcctga cgctgaggcg cgaaagcgtg
      241 gggagcaatc cgg
//
LOCUS       KHUT01008090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008090
VERSION     KHUT01008090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          102 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agggcgcgta ggcgggaagc
       61 taagtccgct gtcaaagccc ggggcttaac cccggaaggt cggtggagac tggttttctt
      121 gaagctgcca gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctgga gcagacttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008091
VERSION     KHUT01008091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacgaggggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggctgttcta
       61 caagtcagga gtgaaagccc aaggcttaac cttggaagcg ctcttgaaac tgtaggactc
      121 gagttcggga gaggatagcg gaattctcag tgtagcagtg aaatgcgtag atattgagag
      181 gaacaccagt ggcgcaagcg gctatctgga ccgacactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008092
VERSION     KHUT01008092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggctgct
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaattg cattcgatac tgagcggcta
      121 gagtacgata gagggaggta gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaataccggt ggcgaaggcg gcctcctgga ttgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008093
VERSION     KHUT01008093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           87 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtttag
       61 taagtctggt ttgaaagctg gtcgcttaac gatcagatgt ggctggaaac tggtaaactt
      121 gaatgcgttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acgtgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008094
VERSION     KHUT01008094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggctaag
       61 caagtccggt gtgaaatccc acggctcaac cgtggaactg cactagaaac tgcttggctt
      121 gagtatcgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cgattactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008095
VERSION     KHUT01008095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgagta ggcggcctcc
       61 taagtcagac gtgaaatccc ccggcttaac ctgggaactg cgtctgatac tgggaagctt
      121 gagtatggga gaggaaggta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gccttctgga ccataactga cgctgatccg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008096
VERSION     KHUT01008096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           86 g           56 t
ORIGIN      
        1 tacgaggggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtgatt
       61 caagtctggt gttaaatacc gaagctcaac ttcggcttgg cattggaaac tggatcgcta
      121 gagtgtgtca gaggtaactg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaagcg agttactggg acattactga cgctgaggaa cgaaagctag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01008097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008097
VERSION     KHUT01008097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           84 g           56 t
ORIGIN      
        1 tacggggggt gctaacgttg tccggaatta ttgggcgtaa agcgcatgta ggtggttttg
       61 taagtcagcg atgaaagacc aacgcttaac gttgggaacg ttgttgatac tgcaaagctt
      121 gaggaaaaca ggggtaagcg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca gcttactggg ttttacctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008098
VERSION     KHUT01008098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttttg
       61 taagtccgat tgtttaaacc cggggctcaa cctcgggtcg caagggaaac tgcaagactt
      121 gagtgcagga gagggaagcg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008099
VERSION     KHUT01008099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggtgcgg
       61 taagtcggac gtgaaatctc cgggcttaac ccggaggggt cgttcgaaac tgccgtgctc
      121 gagtgtggga gagggaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggcgaaggcg gcttcctaga ccacaactga cgctgaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008100
VERSION     KHUT01008100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           58 c          111 g           35 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggttggt
       61 cacatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgagat gggccgactg
      121 gaggcaggga gagggcggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccgcctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008101
VERSION     KHUT01008101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           96 g           38 t
ORIGIN      
        1 gacagaggag gcaagcgttg tccggactta ctgggcgtaa agcgtccgca ggtggtcgtg
       61 caagtgcgct gtgaaagcgc tcggcttaac tgagcgaggc caatgcagac tgcacgactc
      121 gagaccggga gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca gctgactggc ccggtactga cactcaggga cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008102
VERSION     KHUT01008102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatga ctgggcgtaa agggtgcgta ggcggcgcag
       61 caagtcaggc gtgaaatccc cgggctcaac ctgggaactg cgcttgagac tgttgtgctt
      121 gagaccggga gaggatggcg gaatacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccatctgga ccggttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008103
VERSION     KHUT01008103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          103 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaagct
       61 taagtgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcaaac tgggcttctt
      121 gagggggata ggggaaagtg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctttctggg tccgccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008104
VERSION     KHUT01008104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttgac
       61 taagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaagac tggtcgactg
      121 gagagcagta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ctgtatctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008105
VERSION     KHUT01008105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           93 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcaatg
       61 caagtctgaa gtgaaaggac ggggcttaac cctgtgactg ctttggaaac tgtgtagctt
      121 gagtgccgga ggggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cggtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008106
VERSION     KHUT01008106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           78 g           58 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggaggat
       61 taagtcagtg gtgaaagctc tcagcttaac tgggaaattg ccattgaaac tgatcttctc
      121 gagtatggtt gaggttaccg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccgat tgcgaaggca ggtaactaag ccataactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008107
VERSION     KHUT01008107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           91 g           53 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggaccg
       61 taagtccgag aggaaatctc aaggctcaac tttggggctt tcttggatac tgcggttctt
      121 gaggatatca gaggatagta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg actgtctggg atattcctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008108
VERSION     KHUT01008108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           83 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtctg
       61 taagtcagtg gtgaaagccc cgagctcaac ttgggaactg ccattgatac tgctgatctt
      121 gagtatggtt gaggtgggcg gaatgttgcg tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccgat tgcgaaggca gctcactaaa ccattactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008109
VERSION     KHUT01008109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           98 g           45 t
ORIGIN      
        1 cacgggggga gcgaacgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttcgg
       61 taagtgggat gtgaaagccc ccggctcacc cggggaactg catctcatac tgctggactt
      121 gagtgcggga gaggatgggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg cccatctggc ccgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008110
VERSION     KHUT01008110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           96 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtttgc
       61 caagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tggcaggctc
      121 gagtgcggga tgggagagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgc ggtgtagacg gctctctgga ccgccactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008111
VERSION     KHUT01008111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggcggtttgg
       61 caagtcagaa gtgaaagccc aaggcttaac catgggactg cttttgaaac tgtcagactg
      121 gattgcagga gaggtaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttactgga ctgtaaatga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008112
VERSION     KHUT01008112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           77 g           60 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacttg
       61 taagtcagtg gtgaaatcct gcggcttaac tgtagaactg ccattgaaac tgcttgtctt
      121 gagtacattt gaagcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008113
VERSION     KHUT01008113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtccga
       61 caagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgtcggactt
      121 gagtccggga gagggtagtg gaattcccaa tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008114
VERSION     KHUT01008114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          102 g           41 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcactc
       61 taagtacctc gtgaaatctc cctgctcaac ggggagggag cgaagtagac tggggagcta
      121 gaggagggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg cctctcctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008115
VERSION     KHUT01008115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggatttc
       61 caagtcagga gtgaaatccc agggctcaac cctggaattg cttttgaaac tggatgtctg
      121 gagtctcgga gaggttggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgtaagcg gccaactgga cgagaactga cgctcatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008116             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008116
VERSION     KHUT01008116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          100 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctctc
       61 taagtctggt gtgaaaggtt cagggctcaa cccgaacagt gcactggata ctggggagct
      121 tgagtgcggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacccg tggcgaaggc ggctctctgg cctgcaactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008117
VERSION     KHUT01008117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggacttt
       61 taagtcagtg gtgaaatctc ggggctcaac cccgaaactg ccattgatac tattggtctt
      121 gaatatcgtt gaggctggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctggctaag cgatgattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008118             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008118
VERSION     KHUT01008118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           80 g           57 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtaggg
       61 taagttatcg gtcgaagtcg ggctcaactc gatggaggct aataatacta ctttgctgga
      121 ggacaggaga ggttaacgga actctatgcg taggggtgaa atccattgat ctatagagga
      181 ccaccaaaaa aggcgaaggc agttaactat tctgctcctg acattgaggc atgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01008119             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008119
VERSION     KHUT01008119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           75 g           62 t
ORIGIN      
        1 gacataggtt gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtttga
       61 taagttatca gtggtaaatc ggggcttaac ctcgtggaag ctggtaatac tgtcatactc
      121 gagtgcagga gaggttaacg gaactctatg tgtagcggta aaatgtgacg atatatagaa
      181 gaacaccaac aggcgaaagc agttaactat tctgttactg acattgaggc atgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01008120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008120
VERSION     KHUT01008120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           84 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtccgca gccggcgcgt
       61 taagtgtgat gttaaatcgt ggggctcaac cctgtcggtg catcacatac tggcgtgcta
      121 gaggaaatca gaggcaagtg gaattggtag tgtagcagtg aaatgcgtag atattaccag
      181 gaacaccaat ggcgaaggca gcttgctggg atttttctga cggtcatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008121
VERSION     KHUT01008121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcgga
       61 taagtctgat gtgaaagctc tgggcttaac ccagaaatgg cattggatac tattcggctc
      121 gaggtctgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caggtcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008122
VERSION     KHUT01008122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacggggggt gctagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggtctct
       61 taagtcagat gtgaaagccc tcggcttaac cgaggaagtg catttgatac taagtgactt
      121 gagtatggga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccaatactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008123
VERSION     KHUT01008123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           97 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcccgt
       61 taagtcggat gtgaaaaccc gtggctcaac cacgggcctg catccgatac tggcgggctc
      121 gaggcaggca ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008124             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008124
VERSION     KHUT01008124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           54 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctat
       61 taagtgtgaa gtgaaatctc tagggctcaa cccagaaact gcttcgcata ctgatagact
      121 tgaggaatgc agaggtcagt ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc agctgactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008125
VERSION     KHUT01008125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           81 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggcccg
       61 atagtcagtg gtgaaatact ctcgctcaac gagggaactg ccattgatac ttcggatctt
      121 gaatgcagtg gaggcgggcg gaatgtgtca tgtagtggtg aaatacttag atatgacaca
      181 gaacacccat tgcgaaggca gctcgctaca ctgatattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008126
VERSION     KHUT01008126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggatat
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaatgg cgttgaagac tggtattcta
      121 gagtttgata gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgacctgga tcgaaactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008127             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008127
VERSION     KHUT01008127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttac
       61 caagtcgggt gtgaaacctc caggctcaac ctggagctgc cactcgatac tggtatggct
      121 tgagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtggtctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008128             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008128
VERSION     KHUT01008128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           99 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaatctc cgagctcaac tcggagaggc cactcgagac tgttatgact
      121 agagtccggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008129             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008129
VERSION     KHUT01008129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          100 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctctt
       61 taagtctgat ggtcaaatgc cggggctcaa ccccgtgccc gccagggata ctggagagct
      121 ggagacacgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtagtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008130
VERSION     KHUT01008130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgg
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggatac tgccaggctc
      121 gagcgagata ggggtcgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacgactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008131             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008131
VERSION     KHUT01008131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           94 g           51 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggtttga
       61 taagccgggt gtgaaaccct cgagctcaac tcggggcggc cactcggaac tgtcatgact
      121 tgagtcaggt aggggaacac ggaattccag gtgtagcggt gaaatgctta gatatctgga
      181 ggaacaccag tggcgaaggc ggtgttctgg gcctgtactg acgctgatgc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008132
VERSION     KHUT01008132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           40 c           73 g           58 t
ORIGIN      
        1 gacaggggat gcaagtgtta tccagaatca ttgggcgtaa agcgtctgca ggtggacttt
       61 taagtctttt gttaaatctc caggcttaac ctggaatatg caaaagaaac taattgtctt
      121 gagtatagta agggtaaagg gaattcccag tggagcggtt aaatgcattg agattgggag
      181 gaacaccaag ggcgaaggca ctttactagg acaatactga cactgagaga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008133
VERSION     KHUT01008133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtcggtg gtgaaagcct cccgcttaac gggagaagag ctgtcgatac tgggtgtctc
      121 gaggggtgta ggggtctgtg gaactgtggg tggagcggtg aaatgcgtag agatccacag
      181 gaacgccggt ggcgaaagcg acggactgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008134
VERSION     KHUT01008134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           91 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttaag
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaatgg cattcgagac tgcgtagcta
      121 gggtgcggaa gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctggt ccagcaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008135
VERSION     KHUT01008135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 caagtcaggg gtgaaagccc gcagctcaac tgcggaaggg cctttgatac tgccggtctc
      121 gagggatgta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga catcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008136
VERSION     KHUT01008136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggattca ctgggcataa agggcgcgta ggcggttccg
       61 aaagtggcat gtgaaatcct ctggctcaac cagagaagtg cgtgccaaac ttcggttcta
      121 gaggatggta ggggggactg gaactctcgg tggagcggtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctggt ccatcactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008137
VERSION     KHUT01008137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggttccg
       61 taagtgcctt gtgaaatccc atggctcaac catggaattg ctcggtagac tgcggaactt
      121 gaggttggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccaattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008138
VERSION     KHUT01008138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcaggca ggcgggctgt
       61 taagtagaaa gtgaaaggtc ggggctcaac cccaacattg ctttttatac tggcagcctt
      121 gaatcccgga ggggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctttctgga cggggattga cgctcaactg cgaaagccga
      241 ggtagcgaac agg
//
LOCUS       KHUT01008139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008139
VERSION     KHUT01008139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           97 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggtggcccgg
       61 taagtccgcc gtgaaagtcc agggctcaac cctggaaagc cggtggatac tgtcgggctg
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008140
VERSION     KHUT01008140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           91 g           51 t
ORIGIN      
        1 tacgtactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgtgta ggcggccagc
       61 taagtagggt gtgaaaggcc tcggcctaac cgaggcattg cgctctaaac tgactggctt
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tctcgtctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008141
VERSION     KHUT01008141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttag
       61 caagtcagat gtgaaagccc tcggctcaac cgaggaagtg cgtctgaaac tgctgagctt
      121 gagtgtcgga gaggatggtg gaattccaca agtagaggtg aaattcgtag atatgtggag
      181 gaacaccggt ggcgaaggcg accatctgga cgattactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008142
VERSION     KHUT01008142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccatt
       61 caagtcgggg gtgaaagcct gtggctcaac cacagaattg ccttcgatac tgtttggctt
      121 gagtatggca gaggtcagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctgactggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008143
VERSION     KHUT01008143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           85 g           60 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtgggtctc
       61 taagtcggtg gtgaaatctt gcagcttaac tgtaaaattg ccatcgatac tggaggtctt
      121 gagtacggca gagggaaata gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaataccggt agcgaaggcg gtttcctggt ccgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008144
VERSION     KHUT01008144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           62 c          109 g           36 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggtctgc
       61 agagcccggc gtgaaagccc ccggctcaac cgggggaggc cgtcggggac ctgtgggctg
      121 gaggccggta ggggagagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccggacctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01008145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008145
VERSION     KHUT01008145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c           97 g           45 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcgggctgg
       61 tcagtccggg gtgaaatctc acggctcaac cgtgagcggt ccccggatac tgccagtctt
      121 gaggtgtcta gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gctctctggg gaccacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008146
VERSION     KHUT01008146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           99 g           45 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggtggcatgt
       61 caagtactca gtgaaatctc cttgctcaac ggggagggtg ctgagtagac tggcaagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008147
VERSION     KHUT01008147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggccaat
       61 caagtcgggt gtgaaatccc tcggcttaac cgaggaagtg cattcgaaac tgactggctt
      121 gaatactcaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg gagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008148
VERSION     KHUT01008148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           74 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaattg
       61 taagtcagtg gtgaaagccc ttcgcttaac gaaggaactg ccattgatac tgcaaatctt
      121 gagtacattt gatgagggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctctcaaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01008149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008149
VERSION     KHUT01008149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggattta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaactg cgctcaaaac tgccgtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaggcg gctcactggg tctcttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008150
VERSION     KHUT01008150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           84 g           56 t
ORIGIN      
        1 gacgaggggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggcgtac
       61 taagtctgtt gttaaatctc ccggcttaac cgggaatcag caatggatac tgatatgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008151
VERSION     KHUT01008151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           75 g           60 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactta
       61 taagtcagtg gtgaaatcct cccgcttaac gggagaactg ccattgaaac tgtttgtctt
      121 gagtacattt gaagcgggcg gaatgtgtca tgtagaggtg aaattcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008152
VERSION     KHUT01008152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          101 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccggg
       61 taagtagggg gtgaaatctc atggctcaac cgtgaggctg cctcctagac tgcttggctt
      121 gagcatggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccattgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008153
VERSION     KHUT01008153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           85 g           53 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 ttagtctgtt gtgaaagctc cttgcttaac ggggagaggt caacagatac tgccagactt
      121 gagtatagga gagggtactg gaattcctgg tgtagcggtg aaatgcgcag agatcaggag
      181 gaacaccgat ggcgaaagca ggtacctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008154
VERSION     KHUT01008154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           82 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ttgggcgtaa agggtgcgta ggcggcagga
       61 caagtcgagt gtgaaatttc gaggctcaac ctcgaaactg catacgatac tgtcttgctc
      121 gagtgctata gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacaccagt ggcgaaagcg agtctctgga tagcaactga cgctgaagca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008155
VERSION     KHUT01008155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           89 g           56 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaat
       61 taagttatct gtgaaattcc tgggctcaac ctgggcatgt cagataatac tggttgactt
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008156
VERSION     KHUT01008156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           86 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtaggg
       61 taagtttgag gtgaaagctc cgggcttaac ccggaaacag cctcaaatac tattctgctc
      121 gagggttggc ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctaga caacacctga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008157
VERSION     KHUT01008157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           91 g           54 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtttgg
       61 taagtctgat gttaaagatc ggggctcaac cccgggagtg cattggatac tgccagactc
      121 gagtatggta gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaagcg actcgctggg ccattactga cgctgaggaa cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01008158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008158
VERSION     KHUT01008158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           78 g           58 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcagat
       61 caagttagat gcgaaatacc cgggctcaac tcgggaactg catttaatac tgctctgctt
      121 gagtattaga gaagaagctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaaa
      181 gaacgtcagt ggcgaaagcg agcttctaga taattactga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008159
VERSION     KHUT01008159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           85 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcgtaa agcgcatgta ggtggtcaga
       61 taagtctgat gtgaaatccc cggactcaat tcgggaagtg cattggatac tgtttgacta
      121 gagtgtggga gaggtaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gcttactgga ccactactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008160
VERSION     KHUT01008160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           80 g           59 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatatt
       61 taagtcggat gtgaaatccc tgagcttaac ttaggaattg cattcgatac tgggtatcta
      121 gagtatggta gagggaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008161
VERSION     KHUT01008161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcccgg
       61 taagcgtgcg gtgaaagttc gaggctcaac ctcgaatcgg ccgtgcgaac tgctgggctt
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008162
VERSION     KHUT01008162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgcgtgta ggcggtctgt
       61 taagtcggat gtgaaatctc cgggctcaac ccggaaactg catccgatac tggcagactt
      121 gaggatggta gaggtcggtg gaataccagg tgtaccggtg aaatgggcag atatctggtg
      181 gaacaccagt ggcgaaggcg gccgactggg ccaatcctga cgctgagacg cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01008163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008163
VERSION     KHUT01008163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggctccg
       61 taagtgcttt gtgaaagccc ccggctcaac cggggaactg cagggcatac tgcgaggctt
      121 gaggaaacta gaggccggta gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaatgccgaa agtgaagaca gccggctggg gttttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008164
VERSION     KHUT01008164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggccttg
       61 taagttggac gtgaaacccc cgggcttaac tcggggaggc cgttcaatac tgcggggcta
      121 gagggcgtca gaggggagtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggg gcgcccctga cactgagaag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008165
VERSION     KHUT01008165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           75 g           62 t
ORIGIN      
        1 tacgtaggtc ccaagcgtta tccggaatta ctgggcgtaa agagtctgta gctggtctcg
       61 taagtctact ttcaaatccc tcagctcaac tgaggacagg gagtggatac tgcgagtctg
      121 gaagttttca ggggtaagta gaatttccgg tggagaggtg aaattcgttg atatcggaag
      181 gaatgccaaa agcgaaagca gcttactatg aatatcttga cagtgagaga cgaaagtttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008166
VERSION     KHUT01008166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           90 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggccgctagg
       61 aaagtcggat gtgaaagccc cgggctcaac ccgggaagtg cattcgatac tgcctagcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008167
VERSION     KHUT01008167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           97 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaaactc ggggcttaac cctgggcttg cagtgggtac gggcagacta
      121 gagtgcggta ggggagactg gaacttctgg tgtagcggtg aaatgcgcag atatcagaaa
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgtaactga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008168
VERSION     KHUT01008168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggccatc
       61 taagtctggt gtgaaagcct ggggcttaac cccagaagtg cactggatac tgggtggcta
      121 gagggtgaga gaggagatcg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatctgt ggcgaaggcg gatctctggc tcacttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008169
VERSION     KHUT01008169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtccaa
       61 taagtcggtc gtgaaatccc ttggcttaac caaggaactg cgttcgaaac tactggactc
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008170
VERSION     KHUT01008170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           84 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggctgag
       61 taagtctgat gtgaaatctc cgagcctaac tcggaaactg catcggatac tattcagcta
      121 gaggattgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008171
VERSION     KHUT01008171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           88 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgt
       61 taagttatct gtgaaatccc tgggctcaac ctgggcaggt cagataatac tgatagactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008172
VERSION     KHUT01008172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           86 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgtgcgca ggcgggttcg
       61 caagtcaggt gtgaaatctc gaggcttaac ctcgaaattg cgcctgaaac tgcaagtcta
      121 gaatgtcgga gagggaaggg gaatatctca tgtaggggta aaatccgtag atatgagatg
      181 gaacaccaga ggcgaaggcg ccttcctgga cgattattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008173             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008173
VERSION     KHUT01008173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           84 g           52 t
ORIGIN      
        1 gacataggtc gcaaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggcgaga
       61 taagttagta gtggcaaatc ggggctcaac cccgtggaag ctggtaatac tgtctggctg
      121 gagggcagga ggagttaacg gaactccctg tggagcggtg aaatgcgtag atatagggaa
      181 gaacaccaat cagcgaaggc agttaactat tctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01008174             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008174
VERSION     KHUT01008174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           98 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcatt
       61 taagtctgag gtgaaaggtt cagcgctcaa cgtgaacagt gccttggata ctgggtgact
      121 tgagttaggg agagggaagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggcttcctgg cctataactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008175
VERSION     KHUT01008175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggattag
       61 taagttggaa gtgaaagccc ggggcttaac ctcggaactg ctttcaaaac tactagtcta
      121 gagtgaagta gagggtgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccggt ggcgaaagcg gtcacctgga cttcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008176
VERSION     KHUT01008176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttcg
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggatac tgcggagctc
      121 gagggggata ggggtcgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacgactggg tccctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008177
VERSION     KHUT01008177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagttgcgt gttaaatctc aaggctcaac cttgaaaccg ctcgcaatac tgctctgctc
      121 gaggactgta gaggagaatg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg attctctggg cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008178
VERSION     KHUT01008178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagagacg gcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcggc
       61 taagtcggat gtgaaatctc acagcccaac tgtgataggt cattcgaaac tggctggctt
      121 gaggactgga gaggagactg gaattgtcgg tgtagcggtg aaatgcgtag agatcgacaa
      181 gaacaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008179
VERSION     KHUT01008179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           85 g           56 t
ORIGIN      
        1 tacggagggt gcaaacgtta tccggaatca ctgggcgtaa agagcatgta ggtggtttgg
       61 taagtgagtg attaaagacc gatgcttaac atcgggactg ttgcttatac tgcctgactt
      121 gaggaagaca ggggcaagcg gaactttagg tgtaggggtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttgctggg tcttatctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008180
VERSION     KHUT01008180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggattgt
       61 taagtcgggg gtgaaagccc ggggctcaac cccggaattg ccttcgatac tggcagtctt
      121 gagtatggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008181
VERSION     KHUT01008181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctcat
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgaaac tggcgagctt
      121 gagtatggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008182
VERSION     KHUT01008182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccttt
       61 taagtctgat gtgaaagccc ggggcttacc cccggaagtg cattggagac tgggaggcta
      121 gagtaccgga gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008183
VERSION     KHUT01008183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           76 g           66 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggtctat
       61 taagtcagtg gtgaaagttt gtcgctcaac gataaaattg ccattgaaac tggtggactt
      121 gagtatgttt gaggtaggcg gaatgcgtag tgtagcggtg aaatgcttag atattacgca
      181 gaactccgat tgcgaaggca gcttactaaa ccataactga cactgaagca cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01008184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008184
VERSION     KHUT01008184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           96 g           44 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttgt
       61 caagtctggg gtgaaatccc gcggctcaac cgtggaactg ccttagatac tgacgacctc
      121 gagggaggta ggggcgagcg gaacgggtgg tggagcggtg aaatgcgttg atatcactcg
      181 gaactccggt ggcgaaagcg gctcgctggc cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008185
VERSION     KHUT01008185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           78 g           58 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggttgaa
       61 aaagtctact gttaaaaatt gaagcttaac ttcaaagatg ctgtagaaac tttttgacta
      121 gagtatggta ggggtagagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaccaccaat ggcgaaggca ctctactggg ccattactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01008186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008186
VERSION     KHUT01008186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtcgtctcg
       61 caagtcggaa gtgaaatccc ggagcttaac tccggaattg ctcccgatac tgcggggcta
      121 gagtgtatta gaggatggcg gaattcctag tgtagtggtg aaatacgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg atacaactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008187
VERSION     KHUT01008187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgtgg
       61 taagtctcta gtgaaatctc cgggctcaac tcggagtctg ctagggaaac tgccatgctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008188
VERSION     KHUT01008188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctatg
       61 taagtctgct gttaaagagc aaggctcaac cttgtaaagg cagtggaaac tacatagcta
      121 gagtgcgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01008189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008189
VERSION     KHUT01008189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           88 g           44 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttga
       61 caagtcttgt gtcaaagatc agggcttaac cctggaccgg cacaagaaac tattagacta
      121 gagttcggta ggggcagagg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccaaa ggcgaaagca ctctgctggg ccgcaactga cactgagaga cgaaagcgag
      241 gggagcaaat ggg
//
LOCUS       KHUT01008190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008190
VERSION     KHUT01008190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          102 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 taagtgagag gtgaaaggct ggggcttaac tccagcaggg cctctcatac tgccagtctt
      121 gagggcggta gagagaagtg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg ccgttcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008191
VERSION     KHUT01008191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           91 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaatg
       61 taagtcagat gtgaaatccc agggcttaac tctggagctg catctgaaac tatgtttctt
      121 gagtgccgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008192
VERSION     KHUT01008192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           97 g           41 t
ORIGIN      
        1 aacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggctcgg
       61 aaagtcgggt gtgaaaaccc ggggctcaac cccgggcctg catccgatac taacgggcta
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008193
VERSION     KHUT01008193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           93 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttgtc
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tgggcgactt
      121 gagcgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctttctgga ccacggctga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008194
VERSION     KHUT01008194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           96 g           48 t
ORIGIN      
        1 tacgtaggtg gcgaacgttg tccggattta ctgggcgtaa agcgcgcgca ggcggaccga
       61 taagtcagag gtgaaatctc ccggctcaac tgggaggggc cctttgatac tgtcggtctt
      121 gagatgccta gaggagagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ggcgatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008195
VERSION     KHUT01008195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           79 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggtcatg
       61 taagtcagtg gtgaaagctt ccagcttaac tgggaaactg ccattgaaac tgtgtgactt
      121 gagtacaatt gaagtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaaa ttgcaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008196
VERSION     KHUT01008196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           96 g           40 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccga
       61 tcagtctgct gtgaaagtcg aaggctcaac cttcgaaagc cggtggatac tgtcgggcta
      121 gagtccggaa gaggcgggtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gcccgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008197
VERSION     KHUT01008197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c          101 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggaccgc
       61 cacgtgccgt gtgacaaccc atcgcttaac ggtggaaggc cacggtacac gagcggtctg
      121 gaggtaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggt cctattctga cgctgaggcg cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01008198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008198
VERSION     KHUT01008198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 gacgaaggtg gcgaccgttg ttcggaatca ctgggcataa agagcacgta ggcggcaggg
       61 tatgtcgggt gtgaaatccc tcggcccaac cgaggaattg cgcccgaaac taccttgctt
      121 gaggatggaa ggggagcgtg gaattcctgg tggagcggtg aaatgcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg acgctctggt ccattactga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008199
VERSION     KHUT01008199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           99 g           38 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggactgc
       61 caagtcaggg gtgaaagccc gcggctcaac cgcggaagtg cctttgatac tggcggtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008200
VERSION     KHUT01008200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           91 g           43 t
ORIGIN      
        1 tacagagact gcaagcgtta ctcggattca ctgggcgtaa agggagcgca ggtgggcagg
       61 tgtgtcgggc gtgaaatccc ggggcttaac cccggaacgg cgcccgaaac tatctgtctt
      121 gaggattgga gaggcgggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgac ggcgaaggca gcccgctgga caaatcctga cactcaggct cgaaagtatg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01008201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008201
VERSION     KHUT01008201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacgaaccga gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcatc
       61 caagtctgtg gtgaaatccg gcagcttaac tgtcgaactg ccgtggatac tgggtgtctc
      121 gagggaggta ggggcgtgcg gaactattgg tggagcggtg aaatgcgttg atatcaatag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008202
VERSION     KHUT01008202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggtcttt
       61 taagtcagac gtgaaaacta tgggcttaac ctatagactg cgtttgagac tgagagactt
      121 gaggatcgga gagggcagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cgacacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008203
VERSION     KHUT01008203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           81 g           56 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggcggctcta
       61 caagtcaggt gtgaaatccc acagctcaac tgtggaactg catttgaaac tgtagagctt
      121 gaatatggaa gaggatggta gaattcctga tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gccatctggt ccaatattga cgctgagatg cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01008204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008204
VERSION     KHUT01008204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggttggg
       61 gaagttggat gtgaaagctc ccggctcaac tgggagaggt cgttcaaaac tgcccgacta
      121 gaggatggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccttctaga ccatccctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008205
VERSION     KHUT01008205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacagt
       61 caagtcaggg gtgaaatccc acggctcaac cgtggaagtg cctttgatac tgacagtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008206
VERSION     KHUT01008206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          104 g           43 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtccgt
       61 taagttcggt gtgaaagctc ccggctcaac tgggagaggg cactggagac tgggggactg
      121 gaggcgggta gaggctggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacccat ggcgaaggca gccagctggg cccgacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008207
VERSION     KHUT01008207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcacgta ggcggcccga
       61 tcagtccgct gtgaaagtca aaggctcaac ctttgaatgc cggtggatac tgtcgggcta
      121 gaattcggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gctcgctggg acgatattga cgctaaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008208
VERSION     KHUT01008208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 gacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggttctt
       61 taagtccttt gtgaaattcc ggggctcaac tccggatgtg cagaggatac tggagaactg
      121 gagactggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccagttctga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008209
VERSION     KHUT01008209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           77 g           62 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggggaat
       61 taagtcagtg gtgaaagctg gtagcttaac tatcaaattg ccattgaaac tgattttctt
      121 gagtgcggtt gaggtcactg gaatgtaaca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccgat tgcgaaggca ggtgactaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008210
VERSION     KHUT01008210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           85 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtccga
       61 caagttgggt gtgaaagccc tcagctcaac tgaggaactg cgtccaaaac tgtcggactc
      121 gagggagaca taggtgagcg gaacttaggg tggagcggtg aaatgcgttg agatcctaag
      181 gaacaccagt agcgaaagcg gctcactggg tctcttctga cgctgatgca cgaaagctag
      241 gggagcgaag agg
//
LOCUS       KHUT01008211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008211
VERSION     KHUT01008211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgattgtg
       61 caagtctcct gtgaaagccc acggcttaac cgtggaagtg cagaagatac tgctcaattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008212
VERSION     KHUT01008212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtggtg
       61 catgtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tgcatcgctg
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggacactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008213
VERSION     KHUT01008213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           60 c           93 g           40 t
ORIGIN      
        1 gacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaagcg
       61 taagtgtctt gtgaaatccc acggctcacc cgtggaaccg ctgggcatac tgcgcttctt
      121 gggccgttca ggggcgactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggtcgctggg gacgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008214
VERSION     KHUT01008214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggatact
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaacgg cactcgatac tgggtatcta
      121 gagtatagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctaatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008215
VERSION     KHUT01008215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgagtgta ggcgggatcg
       61 tgtgtcggat gtgaaagccc tcggctcaac cgaggaagtg cgttcgaaac tgcggttctt
      121 gagtgtcgga gagggtgacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gtcacctgga cgaacactga cgctgagact cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008216
VERSION     KHUT01008216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           98 g           46 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcggatcgc
       61 caagtctggt gtgaaagctc ccggctcaac tgggagaggg cattggaaac tggtggtctt
      121 ggatctcaca gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca accggctggg tgaagattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008217
VERSION     KHUT01008217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           48 c           81 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggggta
       61 caagtcaact gtgaaatccc gaggctcaac ctcggaacag catttgaaac tatatcccta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008218
VERSION     KHUT01008218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaattg cattggaaac tggcaagcta
      121 gagtacggta gagggtagtg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacatccgt ggcgaaggcg actacctgga ccagtactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008219
VERSION     KHUT01008219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcttgg
       61 taagttgggg gtgaaatccc tgggctcaac ccaggaatgg ccttcaagac tgcttggctg
      121 gaggacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008220
VERSION     KHUT01008220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           64 c          111 g           34 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccagt
       61 caggcccgac gtgaaagccc ccggctcaac cggggagggt cgtcggggac cggctggctt
      121 gagggcggca ggggtcggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccgactggg ccgactctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008221
VERSION     KHUT01008221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgaga
       61 taagtctgat gtgaaatctc cgagcttaac tcggaaaagg cgttggatac tatcccgcta
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008222
VERSION     KHUT01008222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           95 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggttttt
       61 taagtcaggt gtgaaagccc ccggctcaac tggggagtgt catctgatac tgggaaactc
      121 gagtgcagga gaggaaagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgcaactga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008223
VERSION     KHUT01008223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctcc
       61 caagtccggg gtgaaatctt tcggcctaac cggaaaaggg ccttggatac tgggggactc
      121 gaggggggta ggggcaagcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gcttgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008224
VERSION     KHUT01008224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           97 g           40 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggccgatctg
       61 cgcgtggggt gtgaaatctg gccgcttaac ggccaggcgc catcccatac ggcagatctg
      121 gagcgctgca gaggtcgatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtcgactggg cagctgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008225
VERSION     KHUT01008225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgtg
       61 tcagtcggat gtgaaagccc cgggcttaac ctgggaattg cactcgatac tgcacagcta
      121 gagtctggga gagggaaggg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg ccttcctggc ctaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008226
VERSION     KHUT01008226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           75 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcgggtttg
       61 taagtcagtg gtgaaatctc tcagcttaac tgagaaactg ccattgatac tgcaagtctt
      121 gagtacattt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008227
VERSION     KHUT01008227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           81 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagcg gtgaaagacc agggctcaac cctggaactg ccattgatac tgataagctt
      121 gagtaaagtt gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat tgcgtaggca gcttactaag ctttaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008228
VERSION     KHUT01008228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcaggta ggtggtcttg
       61 taagtctgct gtgaaagccc cgggcttaac ctgggaagtg cggcagatac tgcaggacta
      121 gagtactgga tgggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgcactgga cagtaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008229
VERSION     KHUT01008229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttgaa
       61 taagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tgattaacta
      121 gaatacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacgtcgat ggcgaaggca gccacctggc ttgatattga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008230             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008230
VERSION     KHUT01008230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          101 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtggcttgg
       61 caagtcctgt cgtgaaaggt tgcggcccaa ctgcaacact gcggaggata ctgccaggct
      121 tgagggcagc agaggtcggt ggaattcccg gtggagtggt gaaatgcgta gatatcggga
      181 ggaacaccag aggcgaaggc ggccgactgg gctgtacctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01008231             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008231
VERSION     KHUT01008231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggtggcttgg
       61 cacgtctgtc gctgaaatcc cccggctcaa ccggggaact ggcgtggata cgaccaggct
      121 ggagggatgt agggggatcg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gcggtcctgg acatttcctg acactgagac gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008232             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008232
VERSION     KHUT01008232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           68 c          101 g           32 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgg
       61 cacgtccgtc gctgaaatcc cccggctcaa ccggggaagg ggcgcggata cgaccgggct
      121 ggagcgaggt agggggagcc ggaacgtccg gtggagcggt gaaatgcgtt gagatcggac
      181 ggaacgcccg tggcgaaagc gggctcctgg acctccgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008233
VERSION     KHUT01008233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           95 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcaagta ggcggcactg
       61 caagtcgcgt gtgaaagctt ccggctcaac cggaaaagtg cgcgcgaaac tgcagagctt
      121 gagtcccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga tgcgaaggcg gccacctgga agggtactga cgctgagagg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008234
VERSION     KHUT01008234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           83 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggttatt
       61 caagtcaggg gtgaaatcct ggagctcaac tccagaattg cctttgaaac taaataacta
      121 gagtgtcgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgcttaggcg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01008235             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008235
VERSION     KHUT01008235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           76 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcatt
       61 gtaagtcaat agtgaaagcg ttcggctcaa ccgaatatcc attattgaaa ctgcaaagct
      121 agaggacaag agaggttatt ggaattccta gtgtaggagt gaaatccgta gatattagga
      181 ggaacaccga tggcgtaggc agataactgg cttgttcctg acactaaggc acgaaagcgt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01008236             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008236
VERSION     KHUT01008236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           49 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctcaa
       61 caagtctggc gtgaaagccc agggctcaac cctggaagtg cgctggaaac tgtcgagctt
      121 gagtaccgga ggggaaggtg gaattctcga gtgtagaggt gaaattcgta gatattcgga
      181 agaacatcag tggcgaaggc gaccttctgg acggatactg acgctgagac gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008237             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008237
VERSION     KHUT01008237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           56 c           82 g           48 t
ORIGIN      
        1 ccccagcgcc acgagtggtt atcacgatta ttgggcctaa agcgttcgta gccggttggg
       61 taaatctctt gtgaaattgt tcggcttaac cgaacagcgt gcaggagaca ctgctcaact
      121 cgagaccggg aggcgtgtga agtatgtcgt ggggactggt aaaatgggat aatccacgat
      181 agactaccga tggcgaaggc atcacacgag aacggatctg acggtgagga acgaaagcca
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01008238             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008238
VERSION     KHUT01008238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           81 g           61 t
ORIGIN      
        1 gacataggtg gcaagcgtta tccggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 ttagttatcg gtgggaaatc gaggctcaac ctcgtggaag tcggtaatac tattgaacta
      121 gaggattgga gaggttaacg gaatggttca tgtaggggta aaatcctcaa atatgagttg
      181 gaacaccaat tggcgaaggc agttaactat tcagttcctg acgttgaggc acgaaagcat
      241 ggggagcaag acgg
//
LOCUS       KHUT01008239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008239
VERSION     KHUT01008239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          106 g           41 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggttgct
       61 taagtccggc gtgaaagctc ccggctcacc tgggagaggg cgttggagac tgggcgactg
      121 gaggcaggca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccggctggg cctgtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008240
VERSION     KHUT01008240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtctgat gtgaaagctc ggagcttaac tccgaaatgg cattggatac tatctagcta
      121 gaggatcgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008241
VERSION     KHUT01008241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           87 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgagcgca ggcggttatg
       61 taagacagat gtgaaatccc agggcttaac cttggaactg catttgtgac tgcatggcta
      121 gagtgtgtca gaggggggtg gaactccacg tgtagcagtg aaatgcgtag agatgtggaa
      181 gaacaccgat ggcgaaggca gccccctggg ataacactga cgctcaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008242
VERSION     KHUT01008242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           81 g           60 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatctt
       61 taagtcagtg gtgaaatctt caggctcaac ctggaaactg ccattgatac tattggtctt
      121 gaatactgtg gaggtgagcg gaatgtgtca tgtagcggtg aaatgcatag agatgacaca
      181 gaacaccgat tgcgaaggca gctcactacg cagtgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008243
VERSION     KHUT01008243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggatgat
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaactg catcttatac tggtgatcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008244
VERSION     KHUT01008244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           99 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccgag
       61 taagtagggg gtgaaatctc atggctcaac tgtgaggctg ccttctagac tgcttggctc
      121 gagcacagta gaggctagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gctagctggg ctgttgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008245
VERSION     KHUT01008245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctcg
       61 caagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tatgggactc
      121 gagtgtggca gagggaggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gcctcctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008246
VERSION     KHUT01008246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           95 g           44 t
ORIGIN      
        1 tacgaagggc ccgagcgtta atcggaatca ctgggcttaa agcgtacgta ggcgggcacg
       61 caggcgcttt gtgaaatccc tcggctcaac cgaggaactg cttggcgaac cgcgtgcctt
      121 gaggcaagta gaggcggtcg gaacgctcgg tggagtggtg aaatgcgttg atatcgagcg
      181 gaacgccgaa ggtgaagaca ggccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008247
VERSION     KHUT01008247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattgg
       61 taagtccgtg gtgaaatccc cgagcttaac tcgggaactg ccgtggatac tattaatctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcttactacg cgattattga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008248
VERSION     KHUT01008248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 tacgaaggtc ccgagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcggcgtgg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccatgcta
      121 gagtgatgga gaggtagctg gaattcttgg tgtagcagtg aaatgcgtgg agatcaagag
      181 gaacactcgt ggcgaaagcg agctactgga catttactga cgctgaggct cgaaggccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01008249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008249
VERSION     KHUT01008249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggttgtccta
       61 tttgtctggg gtgaaatccc tgagcttaac tcaggaagtg ccccagaaac ggtaggacta
      121 gagtcttaga gaggttcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga taagtactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008250
VERSION     KHUT01008250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 gacagaggtc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcttt gtgaaatccc acagctcaac tgtggaactg cttggcatac tgacggtctt
      121 gagccatcta ggggtcgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggcgactggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008251
VERSION     KHUT01008251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c          101 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcacgca ggcggaaggg
       61 taagtgggtc gtgaaagcgc tcggctaaac cgggcgagga cgattcagac tgcttttcta
      121 gaggcagaca gagggacgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggcgcaagcg gcgtcctggg tctgaactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008252
VERSION     KHUT01008252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtctgc
       61 cacgttcgga gtgacagtcc tcagcttaac tgaggaagcg cttggaagac gggcagactt
      121 gagggctcaa cagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca gtgtcctctt gagtcgctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008253
VERSION     KHUT01008253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggattcc
       61 taagtacctt gtgaaatccc ccggctcaac cggggaactg ctgggtatac tgggtgtctt
      121 gagccaacta ggggctgccg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaac ggtgaaaaca ggcagctggg gttgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008254
VERSION     KHUT01008254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcttag
       61 taagtcaggg gtgaaaggct acggctcaac cgtggaattg cctttgatac tgctgagctt
      121 gaatgaggtt gaggtgagca gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaataccgat tgcgaaggca gctcactgga cctggattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008255
VERSION     KHUT01008255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           81 g           60 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattta ctgggtgtaa agggtgtgta gacggtttgt
       61 taagttcgtt gttaaatctt ccggcctaac tggaagcttg cgatggaaac tggcaaactt
      121 gagggtgaaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaagca gcttcttggt tcacatctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008256
VERSION     KHUT01008256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           97 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcccgt
       61 taagtcgggt gtgaaaaccc ggggctcaac cccgggcctg cattcgatac tgacaggcta
      121 gaggcaggta gggggaagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008257
VERSION     KHUT01008257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagcgta ggttgctcga
       61 caagtcagtg gtgaaagccc agagctcaac tctggaactg ccattgaaac tgtcgagctg
      121 gaatctcaga gggggtagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctacctgga tgagtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008258
VERSION     KHUT01008258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 cacgggggga actagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcgggctga
       61 caagtcgggt gtgaaattcc agggcttaac cttggatctg cattcgaaac tgccggtctt
      121 gagtactgga gaggtgagtg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccgtt ggcgaaggcg gctcactgga cagatactga cgctgaggca cgaaggtcgg
      241 gggagcaaac agg
//
LOCUS       KHUT01008259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008259
VERSION     KHUT01008259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcgggcttt
       61 taagtcgggg gtgaaatcct ggagcttaac tccagaactg ccttcgaaac tgaaagtctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008260
VERSION     KHUT01008260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagacggat gtgaaatccc cgggctcaac ctgggaactg cattcgtgac tggcaggcta
      121 gagtatggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008261
VERSION     KHUT01008261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           89 g           52 t
ORIGIN      
        1 tacagggggt gctaacgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctata
       61 caagtcagat gtgaaatctc ggggcttaac tccgaaagtg catttgaaac tgtatggcta
      121 gagtacagta gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008262
VERSION     KHUT01008262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggcgggttta
       61 caagtcagat gtgaaatctc agggcttaac cctgaaactg cgtctgaaac tgtagaccta
      121 gaatatcata gggggcaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctgcctgga tgattattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008263
VERSION     KHUT01008263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcatgta ggtggctatt
       61 taagtctgat gtgaaagccc cgggcttaac ctgggaattg cgttggaaac tgaatagcta
      121 gagtcggtca gagggccgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcggcctggg accagactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008264
VERSION     KHUT01008264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggtgggttga
       61 taagtgagat gtaaaagccc cgggcttaac ctgggaaagt gattttagac tgtcaacctg
      121 gagtacagta gagggtagaa gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgaaactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008265
VERSION     KHUT01008265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agcgctcgta ggcggctctg
       61 taagtcggaa gtgaaagacc acggctcaac cgtggaaacg ctttcgaaac tgcggagctt
      121 gaggctggga gaggagattg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg aatctctgga ccagtactga cgctgatgag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008266
VERSION     KHUT01008266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           91 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggctata
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaattg cgttgaatac tgtatagctt
      121 gagtcggaca gagggcggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatcggt ggcgaaggcg gccgcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008267
VERSION     KHUT01008267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatca ttgggcgtaa agagcgcgta ggtggatcat
       61 ccagtctgct gtgaaagtca aaggctcaac ctttgaaagc cggtggatac tgttgatcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008268
VERSION     KHUT01008268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttagt
       61 taagttagat gtgaaagccc cgggcttaac ctgggaatgg catctaaaac tggttgacta
      121 gagtatggta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actgcctgga ttaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008269
VERSION     KHUT01008269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaataa ttgggcgtaa agggtgcgta gacggcctgt
       61 taagtctgtt gttaaattct ccggctcaac cggggagctg caatggaaac tggcggacta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccgat ggcgaaggca gcttcttggc tcattcctga cgttgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008270
VERSION     KHUT01008270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 tacagaggcc acaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggacctg
       61 taagtgttct gtgaaatccc tcggctcaac cgaggaattg cagggcatac tgcgggtctt
      121 gaggcatgta ggggcgggca gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggggaagccg gcccgctggg catgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008271
VERSION     KHUT01008271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agcgcgcgta ggcggttata
       61 taagttgggt gtgaaatccc tgggctcaac ctaggaattg cattcaaaac tgtatgactt
      121 gagtgaagta gaggtgagtg gaatttccgg agtagcggtg aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaggcg actcactggg ctttaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008272
VERSION     KHUT01008272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcgtt
       61 taagtagggt gtgaaatccc tcggctcaac cgaggaattg cgccctaaac tggatggctg
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tcttaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008273
VERSION     KHUT01008273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtaggg
       61 taagttcaag gtgaaagctt cgggctcaac ccgaaaattg cctcgaaaac tatcctgcta
      121 gagggtcgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg agtctctgga cgattcctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008274
VERSION     KHUT01008274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacagggggt gcaagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcggaccgt
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaactg cattcgatac tggcggtctt
      121 gaatacggga gaggtgagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactggc ccgatattga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008275
VERSION     KHUT01008275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           39 c           75 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcataa agagttcgta ggcttttttg
       61 aaagtctttg attaaagact caagcttaac ttgaggaagg ttaaagaaac tccaagaata
      121 gaaggatatc gaggtgagtg gaattcccgg tgtagcagtg aaatgcgtta atatcgggag
      181 gaacaccaat ggcgaaggca gctcactagg tatttcttga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008276
VERSION     KHUT01008276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcagga
       61 taagtcggat gtgaaattcc ggggctcaac cccggagcgg catttgaaac tgtccagcta
      121 gagtacagtt agggtaaacg gaattcctcg tgtagcggtg aaatgcgtag atatgaggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctgtaactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008277
VERSION     KHUT01008277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           89 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttata
       61 taagttagat gtgaaattcc tgggcttaac ctggggactg cgtctgatac tgtatagcta
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaggcg accttctgga cagatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008278
VERSION     KHUT01008278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          100 g           46 t
ORIGIN      
        1 gacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggcgggcgta
       61 taagtggttc gtgaaatatc cgggctaaac tcgggtaggt cgaatcagac tgtatgtctg
      121 gaggcgggta gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag atatccggag
      181 gaacaccaga ggtgcagacg gcgccctggg cccgacctga cgctgagacg cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008279
VERSION     KHUT01008279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           75 g           61 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaatcct gttgcccaac aacagaattg ccattgatac tgatggtctt
      121 gagtacattt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008280
VERSION     KHUT01008280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           88 g           53 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggtccgta ggtggtctag
       61 caagtctgat gtgaaatccc atggctcaac cgtggaactg cattggaaac tgttggactt
      121 gagtaccgca gaggtaaagg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg ctttactggg cggttactga cactgaggga cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008281
VERSION     KHUT01008281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgcga
       61 caagtcagag gtgaaaggcc tgggctcaac ctgggaactg cctttgatac tggcgtgctt
      121 gagtccggaa gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gtcacctggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008282
VERSION     KHUT01008282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           84 a           41 c           74 g           54 t
ORIGIN      
        1 gacgtaggat gcaaacgtta tccggaatta ttgggcgtaa agagttcgta gacgaactgt
       61 taagtaggaa gttaaagcct agtgcctaac actagcaagg ctttctaaac tggcagaata
      121 gagtatagca gaggcaagtg gaattctgtg tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gcttgctggg ctattactga cgttcaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008283
VERSION     KHUT01008283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           85 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgtgta ggcggtgggg
       61 caagttgcgt gttaaatctc aaggctcaac cttgaaaccg cacgcaatac tactctgctc
      121 gaggactgta gaggagaatg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg attctctggg cagttcctga cgctgagaca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008284
VERSION     KHUT01008284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           83 g           59 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattaa ttgggcgtaa agggtgtgca gacggtttgt
       61 taagtcactt gttaaattct tcggcctaac tgaaggtgtg caggtgatac tggcgaacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgttcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008285
VERSION     KHUT01008285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtctgc
       61 caagtttgga gtgacagttt ccggcttaac cggaaaaggc cttcgaaaac tggtggactt
      121 gaggacttga gagggagacg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgaa tgcgaaggca gtctcctggc aagttactga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008286
VERSION     KHUT01008286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           99 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccgga
       61 taagtaggag gtgaaatctc acagcttaac tgtgaggctg ccttctagac tgtctggctt
      121 gagcacggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccgttgctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008287
VERSION     KHUT01008287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttac
       61 ccagtcgggg gtgaaatctt tcggcttaac cggaaaattg ccttcgatac tggtaggctc
      121 gaggaaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008288
VERSION     KHUT01008288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggcgcat
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaactg ctcctgatac tgatgtgctc
      121 gaggacggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtaactga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008289
VERSION     KHUT01008289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccgttgatac tatctgtctt
      121 gaatatcgtg gaggtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcccgctaca cggttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008290
VERSION     KHUT01008290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           35 c           82 g           60 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggttgaa
       61 taagttaggt gtgaaattcc tgggcttaac ctgggacagt cgcttaatac tgtttagcta
      121 gagtactaga gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatcagt ggcgaaggcg actatctgga tagatactga cactgaaaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008291
VERSION     KHUT01008291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agagctcgta ggtggcttag
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg cacttgaaac tgcatagctt
      121 gagtacggta gaggtaagcg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gcttactgga ccagtactga cactgaggag cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01008292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008292
VERSION     KHUT01008292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgag
       61 taagttatct gtgaaagccc tgggctcaac ctgggaatgc cagataatac tgtttaactt
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008293
VERSION     KHUT01008293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggttatg
       61 caagatacag gtgaaatccc cgggctcaac ctgggaatgg cctgtgtgac tgcatagcta
      121 gagtgtgtca gagggaggta gaattccacg tgtagcggtg aaatgcgtag agatgtggaa
      181 gaataccgat ggcgaaggca gcctcctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008294
VERSION     KHUT01008294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           85 g           54 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgcc
       61 taagtgtttt gtgaaatccc acggcttaac cgtggaattg cttgacatac tgggtgtctt
      121 gagccactca ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008295
VERSION     KHUT01008295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtcggtg gtgaaatcct ccagctcaac tggagaattg ccttcgatac tggttgtctt
      121 gaggggggta ggggcagacg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggc ggcgaaagcg gtctgctgga cccccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008296
VERSION     KHUT01008296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           79 g           57 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggcaaga
       61 taagtcaatt attaaatctc ctagcttaac taggaatctg tgattgaaac tgacttgctt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008297
VERSION     KHUT01008297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           93 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ttgggcgtaa agagtgtgta ggcggtgctc
       61 caagtctgtc gtgaaatctc ccggcttaac cgggagggtg cggcggaaac tggagtgcta
      121 gagggtggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagaca gctttctgga ccacttctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008298
VERSION     KHUT01008298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtaatg
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gacattgctg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008299
VERSION     KHUT01008299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           82 g           59 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggttgtttga
       61 caagttagtg gtgaaagccc agagcttaac tctggaattg ccattaaaac tgtcaggcta
      121 gagagtgtta gaggtgagtg gaatttctag tgtaggagtg aaatccgtag atattagaag
      181 gaacatcaat ggcgaaggca gctcactggg tcacatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008300
VERSION     KHUT01008300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           80 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggacata
       61 taagtcagtg gtgaaagccc atcgcttaac gatggaactg ccattgatac tgtatgtctt
      121 gaattcggtc gaagtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gctcactagg cctggattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008301
VERSION     KHUT01008301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaagta ggtggtctcg
       61 tttgtctgag gtgaaatccc tgggcttaac ctaggaactg cctcagaaac ggcgagacta
      121 gagtcctgga gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccaga ggcgaaagcg gcggcctgga caggaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008302
VERSION     KHUT01008302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggcgca ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgctaga gaggtgagta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccgga ggcgaaggcg gctcactggc tagacactga cactcatgcc cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008303
VERSION     KHUT01008303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caggtcggat gtgaaatccc agggctcaac cctggaaggg cattcgaaac cgcttcgcta
      121 gagttctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagaaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008304
VERSION     KHUT01008304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttgg
       61 caagtcgggt gtgaaagccc tgagcttaac ttgggaattg cattcgatac tgcctggcta
      121 gagtatggta gagggaagcg gaattccgca tgtagcggtg aaatgcgtag atatgcggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008305
VERSION     KHUT01008305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcga
       61 taagtccgct gtgaaagtcc ggggctcaac cccgggatgc cggtggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008306
VERSION     KHUT01008306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           64 c          102 g           36 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 cgcgtccggg gtgaaatccc acggctcaac cgtggaacgg ccccgggtac gggcggcctc
      121 gaggaaggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008307
VERSION     KHUT01008307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctgag
       61 agggtggggt gtgaaatccc tcggcttaac cgaggaattg cgctccaaac cgcttggctg
      121 gagtgaggca gaggtgagca gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaataccaga ggcgaaggcg gctcactggg cctcaactga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008308
VERSION     KHUT01008308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           34 c           78 g           71 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctat
       61 taagtcagtg tttaaatttt gcagcttaac tgtaggtatg gcattgatac tgatggactt
      121 gaatattaag gaggtagctg gaatttatgg tgtagcggtg aaatgcttag ataccataag
      181 gaacaccgat agcggaggca ggttaccact tgataattga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008309             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008309
VERSION     KHUT01008309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           83 g           52 t
ORIGIN      
        1 tacgtagggc ccaagcgtta tccggaatta ctgggcgtaa agtgcgcgta ggcgtttttg
       61 taagtcggtc gttaaatctc actgcctaac agtgagaccg cgagcgatac tacaagaatt
      121 gagtcttgca ggggctagca gaatttctgg agtaggggtg aaatccgttg ataccagaag
      181 gaataccaat ggcgaaggca gctagctggg caatgactga cgctgagagc gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008310
VERSION     KHUT01008310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgcat
       61 taagtgtgat gttaaatcgt ggggctcaac cctgtcggtg catcacatac tggtgtgcta
      121 gaggacggca gaggcaagtg gaattacgag tgtagcagtg aaatgcgtag atattcgtag
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008311             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008311
VERSION     KHUT01008311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggccgttcgg
       61 taagtcagat gtgaaaactc caggctcaac ttggagaggc catctgatac tgctgtgact
      121 agagtccggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01008312             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008312
VERSION     KHUT01008312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           82 g           53 t
ORIGIN      
        1 gacgaggggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggcatat
       61 taagtccgct gttaaatctt tcggcctaac cggaaatcag cagtagatac tgatcgtgct
      121 agaggatgga agagagaagt ggaattctcg gagtagcggt aaaatgcgta gatctcgaga
      181 ggaacaccaa tggcgaaggc agcttcttgg tccatccctg acgttgagac acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008313             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008313
VERSION     KHUT01008313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           97 g           40 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcaggca ggcgggtcga
       61 taagtcggat gtgaaatctc cctggcttaa ccgggagagt gcatttgaga ctgtcaacct
      121 ggagggcagc agaggagcgt ggaattccgg gtggagtggt gaaatgcgta gagatccgga
      181 ggaacaccag aggcgaaagc ggcgctctgg gctgcacctg acgctgaact gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008314
VERSION     KHUT01008314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 caagttagac gtgaaagccc tgggcttaac ctaggaatag cgtttaatac tggagggcta
      121 gagattggta gagggaaatg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccaga ggcgaaggcg gtttcctggg ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008315
VERSION     KHUT01008315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          109 g           35 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcccga
       61 caagcgtggg gtgaaagtcc ggggctcaac cccggatcgg cctcgcgaac tgtcgggctg
      121 gagcactgta ggggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008316
VERSION     KHUT01008316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           50 c          101 g           49 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtccgct gtgaaagccc gaggcttaac ctcggcgggt cggtggatac tgccagtctt
      121 gaaggtgcta gggggaaatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtttcctggg gcattcttga cgctgatgcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008317
VERSION     KHUT01008317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggctcga
       61 taagtccgct gtgaaagtca agggctcaac ccttgaatgc cggcggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctac ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008318
VERSION     KHUT01008318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           86 g           52 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatca ttgggcgtaa agagggcgta ggtggctata
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgtatagctt
      121 gagtgttgga gaggttatcg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg ggtaactggc caaacactga cactgaggcc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01008319             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008319
VERSION     KHUT01008319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           92 g           54 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agggcgcgta ggcggttcgg
       61 taagccggat gttaaatctc caagctcaac ttggagctgt catccggaac tgctgtgact
      121 agagtctggt aggggaacac ggaatacctg gtgtagcggt gaaatgctta gatatcaggt
      181 ggaacaccag tggcgaaggc ggtgttctgg gccagtactg acgctgatgc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008320
VERSION     KHUT01008320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtttgta ggcggccctg
       61 taagtcgggt gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcggggctt
      121 gaatatggaa gaggagagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctctctggt ccaatattga cgctgagaaa cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01008321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008321
VERSION     KHUT01008321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           86 g           59 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agcgaacgca ggtggtttgg
       61 taagtcaggg gtgaaagtcc acagcttaac tgtggcactg cctctgatac tgcctttctt
      121 gagtagagta gggggaagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ctctaactga cactcatgtt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008322
VERSION     KHUT01008322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           93 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagcttgta ggcggtttgt
       61 cgcgtcgtcc gtgaaaacca gtggcttaac cattggcttg cgggcgatac gggcagactt
      121 gagtatttca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg aaataactga cgctgagaag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008323
VERSION     KHUT01008323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttaa
       61 taagattgat gtgaaatccc tgggcttaac ctaggagctg cattgatgac tgttaagctt
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008324             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008324
VERSION     KHUT01008324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           98 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggtctga
       61 aaagtctatg ggtgaaatgc cggggctcaa ccccgcgcat gcccaggata ctgtcaggct
      121 ggagacacgt agaggcaggc ggaattccgg gtgtagcggt ggaatgcgta gatatccgga
      181 agaacaccag aggcgaaggc ggcctgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008325
VERSION     KHUT01008325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           51 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgcag
       61 aaagtcgggt gtgaaatccc tcagctcaac tgaggaactg cgcccgaaac tactgtgctt
      121 gaggaagata gaggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgtaagcg gcgtactggg tcttatctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008326
VERSION     KHUT01008326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggatagt
       61 taagttagat gtgaaatccc tgggcttaac ctaggaactg catctgatac tggctatctg
      121 gagtgcggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008327
VERSION     KHUT01008327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           92 g           55 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggttcgt
       61 taagtctggt gtaaaagact ggggctcaac cctgggatcg cactggatac tggcggactt
      121 gagtgtggta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaagcg acttgctggg ccataactga cactgaggaa cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01008328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008328
VERSION     KHUT01008328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggactta ctgggcgtaa agggtgcgta ggcgggagct
       61 taagttagtt gtgaaaggcc tgggcttaac ctaggaactg caattaagac tgggcatcta
      121 gagttgagta gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt tgcgaaggcg gctctctgga ctcaaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008329
VERSION     KHUT01008329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggggatc
       61 taagtccgtg gtgaaatacc ttggctcaac caaggaactg cctcggatac tgggtctctc
      121 gaggaagata ggggcttgcg gaacggctgg tggagcggtg aaatgcgttg atatcagtcg
      181 gaactccggt ggcgaaggcg gcaagctgga tcttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008330
VERSION     KHUT01008330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 gacgtagggg gcgagcgtta ttcggaatta ttgggcgtaa agcgctcgta ggcgggatcg
       61 atagtccgtg aagaaagacc tgggctcaac tcagggaacg gcacggatac ttcgattctt
      121 gaggcaatca gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcacctggg gttgttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008331
VERSION     KHUT01008331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           96 g           44 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggtagcg
       61 caagtctgct gtgaaatctc ctagctcaac taggataggt cagtggaaac tgcgcaactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008332
VERSION     KHUT01008332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           52 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acagctcaac tgtggaactg cttggtatac tggcggtctt
      121 gagccagcta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg gctgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008333
VERSION     KHUT01008333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agcgtacgta ggcggcctcg
       61 taagtcggtg gttaaagacc ggggctcaac cccggaaccg ccttcgatac tgcgaggctg
      121 gagggtggca gaggaagtcg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg gacttctggg ccattcctga cgctgaggta cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008334
VERSION     KHUT01008334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttaaa
       61 taagtgagtg gtgaaatccc tgggcttaac ctaggaattg cttctcatac tgtttaacta
      121 gagtacagta gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga ctgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008335
VERSION     KHUT01008335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtctca
       61 ccagtcgaat gtgaaagccc agggctcaac cttggaagtg cattcgatac taggagactt
      121 gagtctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cgaagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008336
VERSION     KHUT01008336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggtggcgcac
       61 taagccacac gtgaaatccc tcagcttaac tggggaactg cgtgtgggac tggagcgctt
      121 gaggacagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgtttctga cactgaaaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008337             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008337
VERSION     KHUT01008337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggtggtttgg
       61 ttagtcggat gtgaaatctc caggctcaac ttggaggggt catccgatac tgccatgact
      121 agagttcggt agaggagtgt ggaattcctg gtggagcggt gaaatgcgca gagatcagga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgatactg acactgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008338
VERSION     KHUT01008338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          101 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttgtg
       61 taagtcacgt gtgaaatccc ggggctcaac tccggggctg catgtgagac tgcatggcta
      121 gagcacagta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ctgttgctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008339
VERSION     KHUT01008339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           80 g           56 t
ORIGIN      
        1 tacgagtggt gcgagcatta ttcggaatca ttgggcgtaa agggtgtgta ggcggtatat
       61 caagtctgtt gttaaatctc ccagcctaac tgggaaaatg cggtggaaac tgatgtacta
      121 gaagatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccaatcttga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008340
VERSION     KHUT01008340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           85 g           55 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggattta ctgggcgtaa agcgcttgta ggcgggctga
       61 tgtgtcgggt gtgaaatccc tgagcttaac tcaggaactg cattcgaaac tattagtctt
      121 gaggaattga gaggagaacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga agcgaaggcg gttctctgga aattatctga cgctgagaag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008341
VERSION     KHUT01008341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c          100 g           41 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccctg
       61 taagtcagac gtgaaagccc tcggctcaac cggggaacgg cgtctgagac tgcagggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008342             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008342
VERSION     KHUT01008342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtacgta ggcggcgcgt
       61 taagtgcaac gcgaaatctg gtggctcaac catctagact gtgttgcata ctggcgtgct
      121 tgaggatggc agaggcaagt ggaattggta gtgtagcagt gaaatgcgta gatattacca
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatatctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008343
VERSION     KHUT01008343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttatt
       61 taagtcgatt gtgaaatccc tgggctcaac cgaggaagtg cagtcgaaac tgaatagcta
      121 gaggatggca gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaatacctgt ggcgaaggcg gccttctgga ccattcctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008344
VERSION     KHUT01008344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           65 c          103 g           33 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcgcag
       61 cgagtccggg gtgaaatcct tcggcctaac cgaagacggg cctcggagac cgctgcgctc
      121 gaggggggca ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg agatcggaag
      181 gaacgccggc ggcgaaagcg gcctgctggg cccctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008345             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008345
VERSION     KHUT01008345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           44 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccacc
       61 gcaagtcgat tgtgaaatct ccgggcttaa cccggaaagg tcaaccgata ctgcggggct
      121 agagtgcaga aggggcaact ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gctgacactg acgctgaagt gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008346
VERSION     KHUT01008346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtcta ggcggccaag
       61 taagtgagat gtgaaatccc ccggcttacc cggggacctg catctcatac tgcttggctt
      121 gagtatggga gaggatgtgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cacatctggc ccaatactga cgctgaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008347
VERSION     KHUT01008347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtcgta
       61 tcagtccagg gtgacagttt ccggcttaac cggaaaagga ctctggagac ggcacgactt
      121 gagggcttca gagggatgtg gaattccagg tggagtggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca acatcctggg gagtcactga cgctcgggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008348
VERSION     KHUT01008348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggtccgca ggcggtttgg
       61 taagtttgac gtgacagttc ctggctcaac tgggaaagtg cgttggaaac tgccagactt
      121 gagggcttca gagggatgcg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca gcatcctggg aagtcactga cgctcaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008349
VERSION     KHUT01008349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggttaca
       61 taagtctgtt gtgaaagtcg gaggctcaac cttcgaatgc caatggatac tgtgtgacta
      121 gagttcggaa ggggctagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gctagctggt acgacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008350
VERSION     KHUT01008350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           87 g           47 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatggc
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctcgacatac tggtcatctt
      121 gagcaaccta ggggcgagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtttgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008351
VERSION     KHUT01008351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggctttg
       61 taagtctgga gtgaaactcc ggggctcaac cccggaactg cttcggaaac tggagagctt
      121 gagtgctcta gagggcagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actgcctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008352
VERSION     KHUT01008352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtaag
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatttatctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg cggtcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008353
VERSION     KHUT01008353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           85 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ttgggcgtaa agcgcacgca ggcggtctgc
       61 gaagtttgga gtgacagttg atggctcaac catcaaagta cttcaaaaac tggtagactt
      121 gagaacgaga gaggaacatg gaactccagg tggagtagtg aaatgcgtag agatctggaa
      181 gaacaccaat ggcgaaggca atgttctggc tcgtttctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008354
VERSION     KHUT01008354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggcggtcctt
       61 caagtcaggg gtgaaagccc agagctcaac tctggaactg cctttgagac tgtaggactt
      121 gggtatggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccattaccga cgctcaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008355
VERSION     KHUT01008355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           76 g           60 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactgt
       61 taagtcagtg gtgaaagctc catgcctaac atggaaattg ccattgatac tgacggtctt
      121 gagtacattt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgtaggca gctcactaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008356
VERSION     KHUT01008356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           87 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggattta ctgggcgtaa agcgcatgta ggcgggcgat
       61 taagtcgggt gtgaaatccc tgggctcaac ctaggaactg catccgaaac tgatcacctt
      121 gagtagtgga gaggaaagcg gaattccggg tgtagaggtg aaattcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gctttctgga cactaactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008357
VERSION     KHUT01008357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcacgca ggcggtcgca
       61 cgcgtttccg gtgccagctc gttgcttacc gacgggagtg ccgggaagac ggtgtgactg
      121 gagcacctca gagggacata gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaataccaat ggcgaaggca gtgtcctggg aggtcgctga cgctgatgtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008358
VERSION     KHUT01008358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           90 g           44 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagcgt
       61 taagtgcctt gtgaaatccc acggcttaac cgtggaacgg ctgggcatac tggcgttctt
      121 gagctgatca ggggtcgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggcgaaagca gacgactggg atcaagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008359             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008359
VERSION     KHUT01008359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttag
       61 caagtcgggt gtgaaatctc caggctcaac ctggagcggc cattcgatac tgctatgact
      121 cgagtccagt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccgg cggcgaaggc ggcactctgg gctggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008360             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008360
VERSION     KHUT01008360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           85 g           50 t
ORIGIN      
        1 tacaggggga gcaagcgtta tccggaatga ttgggcgtaa agcatttgta ggtggctctt
       61 aaagtctact gtcaaatacc aaggcttaac cttggacagg cagtggagta ctcaagagct
      121 agagtgtggt agaggtagag ggaattccat gtggagcggt gaaatgcgta gagatatgga
      181 ggaacaccag cggcgaaggc gctctactgg gccaagactg acactgagaa atgaaagctg
      241 gaggagcaaa tagg
//
LOCUS       KHUT01008361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008361
VERSION     KHUT01008361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttctt
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggagaactg
      121 gaatgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgttattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008362
VERSION     KHUT01008362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           93 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggtgtaa agggtgcgta ggcgggtttt
       61 taagtctggg gtgaaaggct gcggctcaac cgtaggattg ccctggatac tggagatctt
      121 gagtataaca aaggccggta gaattcgtgg tgtagcggtg aaatgcatag atatcacgaa
      181 gaataccagt ggcgtaggcg gccggctggg ttataactga cgctgaggca cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008363
VERSION     KHUT01008363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ttcgaaatca ctgggcttaa agggcgcgta ggcggacatg
       61 aaagtgtttt gtgaaatccc ccggcttaac cggggaattg cttggcaaac tacatgtctt
      121 gagtcaagta ggggtgtgtg gaactcttgg tggagcggtg gaatgcgtag atatcaagag
      181 gaacgccgaa ggtgaagaca gcacactggg cttgaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008364
VERSION     KHUT01008364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtcgcc
       61 taagtcaggt gtgaaatctc gaggctcaac ctcgaaactg cacttgatac tgggtggctt
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008365             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008365
VERSION     KHUT01008365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagtcaggt gtaaaaactc caggctcaac ttggagaggc cacctgatac tgtcgtgact
      121 agagtccggt agaggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008366
VERSION     KHUT01008366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggtgcgg
       61 taagttgggt gtgaaatctc ccggctcaac tgggagaggt cattcaatac taccgtactc
      121 gagagtagaa gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctggt ctatttctga cgctcagacg cgacagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01008367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008367
VERSION     KHUT01008367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggttcgt
       61 taagtcgact gtgaaagccc ttggctcaac caaggaagtg cagtcgaaac tggcaaactt
      121 gagttccgaa gaggatggcg gaatgcccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccagt ggcgaaggcg gccatctggg aggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008368             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008368
VERSION     KHUT01008368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           67 c          100 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggggcgg
       61 cacgtcggcc gttgaaatcc cccggctcaa ccggggaagg ggcgccgata cgaccgccct
      121 cgaggggcgt agggggagcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggctcctgg acgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008369             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008369
VERSION     KHUT01008369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcgggt gtgaaacctc caggctcaac ctggagacgc cactcgatac tgctgtggct
      121 tgagtccggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008370
VERSION     KHUT01008370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggactgt
       61 taagtcgggg gtgaaatcct ggggctcaac cccagaactg ccctcgatac tggcggtctt
      121 gagtgtggaa gaggttggtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccactactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008371
VERSION     KHUT01008371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           98 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgca ggcggttgga
       61 caagtgggat gtgaaatccc cgggcttaac ccgggaactg cattccggac tgtctggctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008372
VERSION     KHUT01008372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c           97 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggttccg
       61 tgcgtcggcg ctgaaatcca tgggcttaac ccatgggcgc gtgccgatac gacgggactt
      121 gagcctctga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag atatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc agagtgctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008373             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008373
VERSION     KHUT01008373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           96 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctaat
       61 taagtctgag gtgaaaggtt caacgctcaa cgtgaacagt gccttggata ctggttggct
      121 tgagtgtggc agaggagcat ggaattcctg gtgtagcggt gaaatgcgta gagatcagga
      181 ggaataccaa tggcgaaggc agtgctctgg gctaacactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008374             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008374
VERSION     KHUT01008374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttctc
       61 caagtccgtt gtgaaatccc cgggcttaac ctgggaatgg cagtggaaac tagaggactt
      121 gaatctggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg ctgagattga cgctgagtgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008375             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008375
VERSION     KHUT01008375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           98 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcatt
       61 taagtctggg gtgaaaggtt cagcgctcaa cgtgaacagt gccttggata ctgggtgact
      121 tgagtgtggg agagggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctgcctgg cctaacactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008376
VERSION     KHUT01008376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggtttat
       61 taagtcagtg gtgaaagcct gtggctcaac catggaattg ccattgaaac tgataaactt
      121 gagtatagtt gaagtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccaat tgcgaaggca gctcgctaaa ctatcactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008377             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008377
VERSION     KHUT01008377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          109 g           36 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattta ttgggcgtaa agggcgcgta ggcggtccgc
       61 agcagtggcg tgtggaatcc cccggctcaa ccgggggggg ccgcgccaga cgggcggact
      121 tgaggtgagt agagggtcgc ggaacgggcg gagtaggggt gaaatccgta gagaccgtcc
      181 ggaacaccgt tagcgaaggc ggcgacctgg gctcattctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cgga
//
LOCUS       KHUT01008378             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008378
VERSION     KHUT01008378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           97 g           45 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcgacg gtgaaatctc cacgctcaac gtggaggcgc ccgttgagac tgctgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008379             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008379
VERSION     KHUT01008379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           84 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtcaga
       61 taagtcagtt gtgaaatcgc tgggcttaac ccatgcaatt gcgattgata ctgtttgact
      121 tgaaacaggt tgaggtaagc ggaatgtggc atgtagcggt gaaatgcata gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg gcctgatttg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008380             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008380
VERSION     KHUT01008380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           80 g           58 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agtgtgcgta ggtggtttaa
       61 caagttactg gtggtaaatc gaggcctaac ctcgtggaag ttggtaatac tattaaacta
      121 gaggaccgga gaggttaacg gaatgctatg tgtagcggta aaatgctcaa atatatggtg
      181 gaacaccaaa cggcgaaggc agttaactat tcggtgcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01008381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008381
VERSION     KHUT01008381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 gacgaggggt gcaaacgtta ttcggaatga ttgggcgtaa agggtgcgca ggcggtacat
       61 taagtcaact gttaaattcc tcagcctaac tgaggcccag cggtagatac tggtgtactt
      121 gagagtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgctcatgca cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01008382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008382
VERSION     KHUT01008382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggctcgg
       61 taagtctgct gttaaagtcc agggctcaac cctgggatgt cggtggatac tgtcgagctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008383
VERSION     KHUT01008383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacgtagggt gcgagcgttg ttcggaatca ttgggcgtaa agcgtgagta ggtggtttgc
       61 taagtcgggt gtgaaatccc tgggctcaac ccaggaagtg cattcgaaac tagctggcta
      121 gaagatggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccagc ggcgaaggcg gccttctgga ccattcttga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008384
VERSION     KHUT01008384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattta
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgtaagtctt
      121 gagtccggaa gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008385
VERSION     KHUT01008385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccac
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgatac tggtggactc
      121 gagcgaggta ggggtagatg gaactgaggg tggagcggtg aaatgtgtag atatcctcag
      181 gaacgccggt ggcgaaagcg atctactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008386
VERSION     KHUT01008386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          101 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgt
       61 taagtctggc gtgaaaggct ccggctcaac cggagacggc cgctggaaac tggcgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cgacctctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008387             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008387
VERSION     KHUT01008387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           99 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggtc gctgaaagcc cccggctcaa ccggggaacg ggcgccgaaa cggccggtct
      121 ggagggacgt agggggacct ggaacatccg gtggagcggt gaaatgcgtt gagatcggat
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtcttctg acgctgagac gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01008388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008388
VERSION     KHUT01008388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggtgcgta ggcggttata
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagag cattgcaaac gatatgactg
      121 gagtagttga gagggtgatg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gtcacctggc aacatactga cgctgaggca cgagagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008389
VERSION     KHUT01008389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           98 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcgggtctt
       61 taagtgaggg gtgaaaggtt cgggctcaac ccgaacactg cctttcatac tggggacctt
      121 gagtgcggga gaggcgagtg gaatggtcgg tgtagcggtg aaatgcgtag atatcgatcg
      181 gaacacccat ggcgaaggca gctcgctggc ccgtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008390
VERSION     KHUT01008390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           51 c          105 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggccgc
       61 caagtctgct gtgaaattct ggggcttaac ctcagtaggt cggtggatac tggtggtctt
      121 gaaggcgcta gggggaggtg gaattgccgg tgtagcggtg aaatgcgtag atatcggcag
      181 gaacaccggt ggcgaaggcg gcctcctggg gcgtacttga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008391
VERSION     KHUT01008391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggcttga
       61 taagtctgct gtgaaagtcc agggctcaac cctggaatgc cggtggatac tgtcaagcta
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008392
VERSION     KHUT01008392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggtgcgta ggcggttgta
       61 tgagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac ggtacagcta
      121 gagtatgtga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc acaaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008393
VERSION     KHUT01008393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           91 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgtacgta ggcggtccag
       61 caagtcgggt gtgaaatacc tgggcttaac cgaggaagtg caaacgaaac tactggactt
      121 gagtactgaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg cagatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008394
VERSION     KHUT01008394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcggggtgg
       61 caagtctgtg gtgaaatcct ccggctcaac cggagaactg ccgtggatac tgccattctc
      121 gaggggtgta ggggcatgtg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg acgtgctgga cacctactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008395
VERSION     KHUT01008395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           89 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtgaca
       61 tttgtctggg gtgaaatccc tgagcttaac tcaggaagtg ccttggaaac ggtgtcacta
      121 gagtgctgga gaggttcgca gaattcttgg tgtagcggtg aaatgcgtag agatcaagag
      181 gaataccaga ggcgaaggcg gcgaactgga cagttactga cactgaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008396
VERSION     KHUT01008396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctcgg
       61 aaagtcggat gtgaaaactc gaggctcaac ctcgagcttg catccgatac tgccgggctt
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01008397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008397
VERSION     KHUT01008397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacggagggt gcaaacgtta tccggaatca ctgggcgtaa agcgcgtgta ggtggttttg
       61 taagtgggtg attaaatacc gaggcttaac ctcgggaatg ttgtctatac tgcaagactt
      121 gaggaagata ggggcaagcg gaactttggg tgtaggggtg aaatccgtag atatccaaag
      181 gaacaccaat ggcgaaggca gcttgctggg tcttatctga cactgagacg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008398
VERSION     KHUT01008398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcagc
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctttgatac tggctgcctc
      121 gagggaggta ggggcgtgcg gaactttggg tggagcggtg aaatgcgtag atatccaaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008399
VERSION     KHUT01008399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 caagtcggag gtgaaatccc acagcttaac tgtggaagtg cccccgatac tgccggtctg
      121 gagggagata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008400
VERSION     KHUT01008400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           99 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtggtg
       61 tgagtcgggc gtgaaagccc cgggcttaac ctgggaattg cgttcgatac cgcatcgcta
      121 gagtctggga gagggaaggg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg ccttcctggc ctaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008401
VERSION     KHUT01008401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           95 g           41 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccttg
       61 aaagtcgggg gtgaaatccc acggcctaac cgtggaagtg cctccgatac tgcaaggctc
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctctgctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008402
VERSION     KHUT01008402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcag
       61 caagtcgggg gtgaaatctt tcggctcaac cggaaaagtg ccttcgatac tgctgagctc
      121 gaggaagata ggggccacgg gaacgaccag tggagcggtg aaatgcgttg agattggtag
      181 gaacgccggt ggcgaaagcg cgtggctgga tcttccctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008403
VERSION     KHUT01008403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           99 g           35 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggccgat
       61 cacgtggggt gtgaaagcgc gcggcttaac tgcgcgaggc catcccatac ggagcggctc
      121 gagccatgca gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg catcggctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008404
VERSION     KHUT01008404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           98 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggccacg
       61 taagtcggat gtgaaaaccc gaggctcaac ctcgggcctg cattcgatac tgcgcggcta
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01008405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008405
VERSION     KHUT01008405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           96 g           44 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggccgca
       61 ttagtcggat gtgaaaaccc agggctcaac cctgggcctg catccgatac tgtgcggctc
      121 gaggcaggca ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008406
VERSION     KHUT01008406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttttg
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaactg cattcgagac tgcatgactg
      121 gagtattgga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg actgcctggc caaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008407
VERSION     KHUT01008407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgccctg
       61 caagtcaggt gtgaaagccc tgggctcaac ctaggaagtg cacttgaaac tgcggggatc
      121 gagggctgga gagggaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008408
VERSION     KHUT01008408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           42 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgag
       61 caagtcggac gtgaaatccc ccggcttaac tggggaactg catccgatac tgctcggctt
      121 gagtcctgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga caggtactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01008409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008409
VERSION     KHUT01008409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          105 g           32 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggctcac
       61 gacgtgccgt gtgacaggcg ccggctcaac cggcgcaggc cagggtacac gagtgagctg
      121 gaggcagaga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc tctgtcctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008410             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008410
VERSION     KHUT01008410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcaggt gtgaaatctc cgggctcaac tcggagaggc cacctgatac tgctatgact
      121 tgagtccggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008411             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008411
VERSION     KHUT01008411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           49 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggattta ctgggcgtaa agggtgcgta ggcggtcgcg
       61 caagtctggt gtgaaatctt caccactcaa cgggaagagg tcactggaaa ctgcgcgact
      121 tgagtttggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacaccgg tggcgaaggc ggcttgctgg gccaatactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008412             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008412
VERSION     KHUT01008412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           50 t
ORIGIN      
        1 tacgtatggt gcaaacgttg ttcggaatca ctgggcataa agagcacgta ggcggttggg
       61 taagtctgac tgtgaaatcc ctcggcctaa ccgaggaact gcatcggata ctgcctgact
      121 cgagtacagc aggggaaagt ggaattcctg gtggagcggt gaaatgcgta gatatcagga
      181 ggaacgccgg tggcgaaagc gactttctgg gctgttactg acgctgaggt gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008413
VERSION     KHUT01008413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttaggt gtgaaagccc tgggcttaac ctgggaactg cgcctaatac tgtcaagcta
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008414
VERSION     KHUT01008414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtgggc
       61 taagtcggat gtgaaagccc agggctcaac cctggaagtg cactcgatac tggctcgcta
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gccaactgga cagatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008415
VERSION     KHUT01008415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           96 g           42 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccgt
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg cctcggatac tggcggtctc
      121 gagcgaggta ggggcaggcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaggcg gcctgctgga cctctcctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008416
VERSION     KHUT01008416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggcgcaa
       61 taagtttgat gtgaaatccc tgggcttaac ctgggaatgg cgttgaagac tgttgggcta
      121 gagtttgaca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgacctggg tctaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008417
VERSION     KHUT01008417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcgca
       61 caagtcaggt gtgaaatccc ccggctcaac cggggaattg cgcttgaaac taggtgactg
      121 gagtgaggta agggtacgcg gaacttctgg tggagcggtg aaatgtgtag atatcagaag
      181 gaacgccggt ggcgaaagcg gcgtactggg ccttgtctga cgctgaggca cgaaagctag
      241 ggaagtgaac ggg
//
LOCUS       KHUT01008418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008418
VERSION     KHUT01008418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          103 g           37 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtcgtgc gtgaaaggct ccggctcaac cggagcaggc cgcccgaaac ggccgttctt
      121 gagggtcgca gaggagcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcgctctggg cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008419
VERSION     KHUT01008419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           89 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggatatg
       61 taagttagat gtgaaatacc taggcttaac ctgggaggtg catttaagac tgcatgtcta
      121 gagtttgtca gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca gccccctggg atagagctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008420
VERSION     KHUT01008420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggtctgt
       61 taggccggct gtgaaagccc atggctcaac catggaaggg cagccggaac cggcaggctt
      121 gagttctgac gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccgcctggg aagcgactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008421
VERSION     KHUT01008421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           88 g           45 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggatcag
       61 taagtcagat gtgaaatctc agggctcaac cctgaaactg cgtctgaaac tactagtcta
      121 gagtgtcgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctacctgga cgactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008422
VERSION     KHUT01008422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           84 g           55 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcgagt gtgaaagcct ggggctcaac cccagaagtg cattcgatac tgcgaagctt
      121 gagtggtaga gagattagta gaattgttgg tgtagtggtg aaatacgtag atatcaacag
      181 gaataccggc ggcgaaggcg gctaattggc tacacactga cactcagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01008423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008423
VERSION     KHUT01008423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgtgg
       61 aaagtcagat gtgaaatctg gaggctcaac ctccaaatgg catccgatac taccatgcta
      121 gagtattgga gagggatctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagag
      181 gaacactagt ggcgaaggcg agatcctgga caatgactga cgctgaggca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008424
VERSION     KHUT01008424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           97 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgact gtgaaagccc ggggctcaac tccgggtctg cagtcgatac gggcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008425
VERSION     KHUT01008425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcctcg
       61 taagtggtgt gtgaaatctc ctggctcaac cagggaattg cgcaccaaac tgcgaggctt
      121 gaggacagta gaggaagctg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgtt ggcgaaggcg agcttctggg ctgttcctga cgctgaagtg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01008426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008426
VERSION     KHUT01008426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c          100 g           44 t
ORIGIN      
        1 tacagggggt gcgagcgtta atcggattta ctgggtgtaa agggcgcgta ggcggcatga
       61 tgtgagcggt gtgaaagccc cgggcttaac ctgggaagag caccgcagac tgtcgtgctg
      121 gagtacatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008427
VERSION     KHUT01008427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgtt
       61 taagtgaggt gtgaaatccc cgggcttaac ctgggacgtg catcttatac tgggcaactg
      121 gagtgcaaga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ttgacactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008428
VERSION     KHUT01008428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggatcgt
       61 taagtccggt ctgaaagccc ccggctcaac cggggagggt ggctggaaac tggcgatctt
      121 gagggcgata gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctgga tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008429
VERSION     KHUT01008429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           96 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtccga
       61 tttgtctggg gtgaaatccc agggcttaac cctggaagtg ccccagaaac ggtcggacta
      121 gagtgctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtgactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008430
VERSION     KHUT01008430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           78 g           59 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtggaa
       61 taagttattg gcagaagtcg agctcaactt gacggaagtt aataatactg ttctacttga
      121 ggacagaaga ggttggcgga actttatgag taggggtgaa atccattgat tcatgcggga
      181 ccaccaaaga ggcgaaggca gctaactatt ctgctcctga cattgaggca tgaaagcgtg
      241 gggagcaaaa cgg
//
LOCUS       KHUT01008431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008431
VERSION     KHUT01008431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgac gtgaaatctc gcggctcaac cgcgaaactg cgtcggatac tatccggctt
      121 gaggagtgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cacttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008432
VERSION     KHUT01008432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           95 g           49 t
ORIGIN      
        1 tacggaggcc ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtatgg
       61 taagtctggt gtgaaaggcg ggggctcaac ccccggactg cattggatac tgccgtacta
      121 gaagactgga gaggagaatg gaattcacgg tggagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaggcg attctctgga cagtacttga cgctgaggca cgaaggctag
      241 gggagcgaac agg
//
LOCUS       KHUT01008433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008433
VERSION     KHUT01008433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgac
       61 taagtcagac gtgaaatccc tcggcttaac cgaggaactg cgtctgatac tgatcggctt
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacactga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008434
VERSION     KHUT01008434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          109 g           31 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtag agcgcgcgca ggcggctcgc
       61 gacgtgccgt gtgacaagcg ggggctcaac ccccgagggt cacggtagac gcgcgggctg
      121 gaggcagaga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc tctgacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008435
VERSION     KHUT01008435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggtatg
       61 aaagtttgat gtgaaagccc tgggcttaac ctgggaacgg cgttgaatac tatatatcta
      121 gagtcggaca gagggtcgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcggcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008436
VERSION     KHUT01008436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           92 g           42 t
ORIGIN      
        1 gacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggagggc
       61 taagtacctt gtgaaatccc cgggctcaac ccgggaactg ctgggtatac tggccttctt
      121 gaggcatcta ggggcgagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggagaaatca gctcgctggg gatgtcctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008437
VERSION     KHUT01008437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           92 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctta
       61 tttgtctagg gtgaaagcct cgggctcaac ccgagaagtg ccttagaaac ggtaagacta
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caggaactga cactaaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008438
VERSION     KHUT01008438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           97 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgata
       61 tgggtgtgat gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac tatatgactg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttgatactga cgctgaggcg cgagagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008439
VERSION     KHUT01008439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           97 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggccgtt
       61 taagtccgat gtgaaaggtc ccggctcaac cgggagacgg catcggagac tgatcggctt
      121 gaaggtggga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gctacctggc ccattcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008440
VERSION     KHUT01008440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggccatc
       61 taagtctggt gtgaaagccc ggggctcaac cccggaaggg cactggatac tggaaggcta
      121 gaggatatga ggggagattg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatcagt ggcgaaggcg aatctctggc atatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008441
VERSION     KHUT01008441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           91 g           45 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctctt
       61 caagtcaggt gtgaaagccc ccggctcaac cggggaactg cgcttgaaac tgaagagctc
      121 gagtcagcta gagggacacg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccgga ggcgaaagcg gtgtcctggg gctggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008442
VERSION     KHUT01008442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtgaag
       61 catgtcagat gtgaaagccc tgggcttaac ctaggaattg catttgaaac tgcttcgcta
      121 gaggccggaa gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctggt ccggatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008443
VERSION     KHUT01008443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggcatt
       61 caagtcgggg gtgaaatccc ggggctcaac cccggaactg ccttcgatac tggatgcctg
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008444
VERSION     KHUT01008444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggttacg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaagtg cactcgaaac tgcgtgactt
      121 gagtcccgga gaggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccatctgga cggtgactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008445
VERSION     KHUT01008445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggggcat
       61 taagtcagtg gtgaaatctc tcagctcaac tgagaaactg ccattgatac tgatgttctt
      121 gagtttggtt gaagtaggcg gaatgaggca tgtagcggtg aaatgcatag atatgcctca
      181 gaacaccgat cgcgaaggca gcttactaag ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008446
VERSION     KHUT01008446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatca ttgggcgtaa agcgcgcgca ggcggccatg
       61 caagtctgac gtgaaatccc ggggctcaac ctcggaactg cgttggaaac tgtatggctt
      121 gagtttggga gaggttcgtg gaattccagg tgtaggggtg aaatccgtag agatctggag
      181 gaacaccagt ggcgaaggcg acgaactgga ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008447
VERSION     KHUT01008447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           91 g           49 t
ORIGIN      
        1 tacggaggct cctagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggaccgg
       61 tcagtctgga gtggaatccc ggggctcaac ctcggaaggc cttcggatac tgccggtctt
      121 gagttcgtga gaggaaagcg gaattcaggg tgtagcggtg aaatgcttag atatcctgag
      181 gaacaccaat ggcgaaagca gctttctggc gcgaaactga cgctgaggct cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008448
VERSION     KHUT01008448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           89 g           54 t
ORIGIN      
        1 tacggagtgt gctagcgtta ctcggaatca ctgggcataa agcgcacgta ggcggagtgg
       61 tatgtcggtg gtgaaatccc ccagcttaac tggggaattg ccttcgaaac tgcttctctt
      121 gagggtgaga ggggagagcg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg gctctctggc tcatttctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008449
VERSION     KHUT01008449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggttttc
       61 taagtcaggt gtgaaatcct cgggctcaac ccgagaactg catttgatac tggaagacta
      121 gagtgcggta gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccatctgga ctgacactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008450
VERSION     KHUT01008450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          102 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtgcgg
       61 taagttgggc gtgaaatccc cgggcttaac ccgggggggt cgttcaaaac tgccgtgcta
      121 gggtatggga gagggtagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggcgaaggcg gctgcctgga ccattaccga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008451
VERSION     KHUT01008451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c          102 g           45 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggccctg
       61 taagtcttgt gtgaaatctc ccggctcaac tgggagggtg catgggaaac tgctgggcta
      121 gagactcgaa gggggatgcg gaattcccgg tggagcggtg aaatgcgttg atatcgggag
      181 gaacatcgga ggcgaaggcg gcatcctggt cgaggtctga cgctgaagcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008452
VERSION     KHUT01008452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           94 g           42 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtcggtg gtgaaatctt tcggctcaac cggaaaagtg ccgccgatac tggcagcctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008453
VERSION     KHUT01008453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           60 c          109 g           37 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgtgcgca ggcggtctgt
       61 cgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggcagactt
      121 gagggtgtca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg acacacctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008454
VERSION     KHUT01008454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           93 g           43 t
ORIGIN      
        1 tacgtagggt gcaagcgtta ctcggaatca ctgggcgtaa agggtgcgta ggcggctcat
       61 taagttggat gtgaaagccc tcggctcaac cgaggaactg cgtccaaaac tggcgagctc
      121 gaggatggta ggggatagtg gaactccagg tggagcggtg aaatgcgtag agatctggag
      181 gaacgccggt ggcgaaagcg actatctggg ccattcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008455
VERSION     KHUT01008455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           51 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggttgtg
       61 ttagtctctt gtcaaatttc ccggcttaac cgggaacctg caggggaaac ggcacgacta
      121 gaggatgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca attcactggc gcattcctga cgctgagaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008456
VERSION     KHUT01008456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattca ctgggcgtaa agcgcgcgta ggcggacttt
       61 taagtcagag gtgaaatgcc gaggctcaac ctcggaactg cctttgaaac tgggagtctt
      121 gaggtatgga gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008457
VERSION     KHUT01008457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           90 g           54 t
ORIGIN      
        1 tacggaggcc tcgagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggaacgg
       61 taagtacttt gtgaaatccc cggcttcaat ccgggaactg cttggtatac tgtcgttctt
      121 gaggcaggta ggggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01008458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008458
VERSION     KHUT01008458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           91 g           45 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggcctgt
       61 taagtctcct gtgaaactcc ggggctcaac tccggacatg cagaagatac tggcaggcta
      121 gagatcggta gaggcgagtg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacacctgt ggcgaaggcg actcgctggg ccgaatctga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008459
VERSION     KHUT01008459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           96 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggccgga
       61 agagtgtggt gtgaaagccc tgggcttaac ctgggaagtg cattgcaaac gttctggctg
      121 gagtagatga gagggtgatg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacgtcgat ggcgaaggca gtcacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008460
VERSION     KHUT01008460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggttcgta ggcggcttgg
       61 gcagtcggac gtgacaatgc gcggctcatc tgcgcgaggt cgttcgaaac tcccaggcta
      121 gaatcggtgc gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggaa
      181 gaacctcaaa ggcgcaggca gccggctagt gccagattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008461
VERSION     KHUT01008461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           98 g           42 t
ORIGIN      
        1 tacgagtggg gcaaacgttg ttcggaatca ctgggcataa agggcgtgta ggcggtccgg
       61 aaagttgtgg ttgaaatccc ccggctcaac cggggaactg gctgcaaaac tgacggactg
      121 gaggatggca ggggagtgtg gaacgatcgg tggagcggtg aaatgcgtag atatcggtcg
      181 gaacaccggt ggcgaaggcg acactctggg ccattcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008462
VERSION     KHUT01008462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcacgta ggcggcctgt
       61 taagcaagat gtgaaagccc cgggctcaac ctgggaatgg cattttgaac tggcaggctc
      121 gagttctgaa gagggtggta gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaataccggt ggcgaaggcg gccacctggt cagaaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008463
VERSION     KHUT01008463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggatat
       61 taagtcagtg gtgaaatctt ccagcttaac tgggaaactg ccattgatac tgatatcctt
      121 gagtttgatt gaagtaggcg gaatgaggca tgtagcggtg aaatgcatag atatgcctca
      181 gaacaccgat cgcgaaggca gcttactaag tcaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008464
VERSION     KHUT01008464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcgggtcag
       61 caagtctttc gtgaaagctc ccggctaaac tgggagaggt cggaagatac tgctagccta
      121 gagtatggca gaggtgtgtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gcacactggg ccataactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008465
VERSION     KHUT01008465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttaggtttaa agggtgtgta ggcggatttc
       61 taagtcagtg gtgaaatccc gcagcttaac tgcggaactg ccattgatac tggtaatctt
      121 gagtacagtt gaagtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactaag ctgttactga cgctgatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008466
VERSION     KHUT01008466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           86 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggtttg
       61 taagtcaatt gtgaaagccc cgggctcaac ctgggaattg cagttgaaac tgcaaacctt
      121 gaatgcatga gaggatagtg gaattcccgg tgtagaagtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctatctggc actgcattga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008467
VERSION     KHUT01008467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggtggcttat
       61 taagtctggt gtgaaagccc agggctcaac cctggaactg cattggaaac tagtaggctt
      121 gagtccaaga ggggtcagta gaattcctgg tgtaggggtg aaatccgtag agatcaggag
      181 gaataccggt ggcgaaggcg actgactgga ttggaactga cactgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008468
VERSION     KHUT01008468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggctaga
       61 tgtgtcaggt gtgaaatccc gaggctcaac ctcggaactg cgcctgaaac tatttagcta
      121 gagtactgga gagggtaacg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gttacctgga cagttactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01008469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008469
VERSION     KHUT01008469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           99 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcgcat
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaactg cgtctgggac tggtgggcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008470
VERSION     KHUT01008470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           83 g           55 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattta ttgggcgtaa agggtgcgta gacggtttgt
       61 taagtctgtt gttaaatcct ccgacctaat cggagatcgg cagtggaaac tggcaatcta
      121 gagtgtgaaa gagagaagtg gaattcccgg agtagcggta aaatgcacag atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggt tcacaactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008471
VERSION     KHUT01008471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           92 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggcttga
       61 taagtttcag gtgaaatctc ccggctcaac tgggaggctg cctggaaaac tattaggctt
      121 gaggcaggga gagggatgca gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008472
VERSION     KHUT01008472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           60 c           95 g           39 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggattca ctgggcttaa agggcgcgta ggcgggcaac
       61 caagtcgggg gtgaaatcct gccgcccaac ggcagaaggg ccctcgatac tggctgtcta
      121 gagcagggta ggggcacgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008473
VERSION     KHUT01008473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 gacaggggcc ccaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggccgt
       61 taagtgtctt gtgaaatccc ccggctcaac cggggaattg cagggcaaac tggcggcctc
      121 gagacaagta ggggcgggaa gaacagtcgg tggagcggtg aaatgcgtag atatcgactg
      181 gaatgccgat ggtgaagacg tcccgctggg cttgttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008474
VERSION     KHUT01008474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgac
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggccggctg
      121 gagggaggta ggggcaggtg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg acctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008475
VERSION     KHUT01008475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           83 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggatagg
       61 caagttagat gtgaaagccc cgagcttaac ttgggaattg catttaagac tgcctgtcta
      121 gagtttgtca gaggggggtg gaattccatg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccaaa ggcgaaggca accccctggg ataaaactga cgctcatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008476
VERSION     KHUT01008476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc ccggctcaac cggggaacgg ctgggtatac tggcgatctt
      121 gagccaccta ggggcagtcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca gactgctggg ggtatgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008477
VERSION     KHUT01008477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcttaa agggtgtgta ggcgggtcga
       61 caagtgtctg gtgaaagccc cgggctcaac ccgggaacgg ctgggcatac tgacgatctt
      121 gaggcaggta gaggtcactg gaacaactgg tggagcggtg aaatgcgtag atatcagttg
      181 gaacgccgga ggcgaaagcg ggtgactggg cctgtcctga cgctgaaaca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008478
VERSION     KHUT01008478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           77 g           65 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaagaa
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaactg ccattgatac tgtttttctt
      121 gagtgcgatt gaagtaggcg gaatgtgtaa tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgtaggca gcttactaag tcgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01008479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008479
VERSION     KHUT01008479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           95 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcccgca ggcggcccaa
       61 taagtttcag gtgaaatctc ccggctcaac tgggaggggt cctggaaaac tgttgggctt
      121 gaggcaggga gagggatgcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008480
VERSION     KHUT01008480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          109 g           34 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 taagcgtggg gtgaaagtcc ggggctcaac cccggatcgg ccttgcggac tgccgagcta
      121 gagcaccgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccgga ggcgaaggcg gcccgctggg cggtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008481
VERSION     KHUT01008481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           96 g           36 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggcacg
       61 cgagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac cgcgagtctc
      121 gagggggata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tccctactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008482
VERSION     KHUT01008482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           84 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcgtaa agcgcatgta ggttgtttca
       61 taagtctgat gtgaaagccc tggactcaat tcaggaagtg cattggatac tgtgagacta
      121 gagtgtggga gaggtaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gcttactgga ccactactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008483
VERSION     KHUT01008483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggccatc
       61 taagtttggt gtgaaagccc agggcttaac cctggaagtg cactgaatac tggacggcta
      121 gagggtgaga ggggagtttg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatcagt ggcgaaggcg aaactctggc tcatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008484
VERSION     KHUT01008484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           96 g           51 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcctgg
       61 taagtctgac gtgaaatgta ccggttcaac cggggcactg tgtcggaaac tgcctggctt
      121 gaggtcgagg gaggaaggta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacccgt ggcgaaggcg gccttctatc tcgattctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008485
VERSION     KHUT01008485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           89 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaataa ttgggcgtaa agggtgcgca ggcggtaagc
       61 taagacggat gtgaaagccc cgggcttaac ctgggaatgg catacgtgac tggctcacta
      121 gaatatggca gagggaggta gaattccaca tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gcctcctggg tcaatattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008486
VERSION     KHUT01008486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           82 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggtggcaaaa
       61 aaagtctggt gtgaaatctc cgagcttaac tcggaaactg cactagaaac tgttctgcta
      121 gagtgttgga ggggagattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg aatctctgga caactactga cgctgatgcg cgaaagctag
      241 gggatcaaac ggg
//
LOCUS       KHUT01008487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008487
VERSION     KHUT01008487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacagagacg gcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggtcggg
       61 taagtcggat gtaaaagctc acagcccaac tgtgataggt cattcgaaac tgcccggctc
      121 gaggaatgga gaggagactg gaatggtcgg tgtagcggtg aaatgcgtag agatcgacca
      181 gaacaccggt ggcgaaggcg ggtctctgga catttcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008488
VERSION     KHUT01008488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtgggta ggtggtcttg
       61 tttgtctaag gtgaaatccc cgggcttaac ctgggaactg ccttggaaac ggcaagactg
      121 gagtgctaga gagggtcgtg gaattcctgg tgtagcggta aaatgcgtag atatcaggag
      181 gaacaccaga ggcgaaggcg gcggcctggc tagcaactga cactgaacca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008489
VERSION     KHUT01008489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggtcggg
       61 ttagtcaggt gtgaaagctc gtcgcttaac gacgaaatgg cacttgatac tgctcggcta
      121 gaggatcgga gggggcaacg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgtaggcg gttgcctgga agattcctga ctctgaaaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008490
VERSION     KHUT01008490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctgag gtgaaagcct tgagcttaac tcaagaagtg ccttagaaac ggtgagactg
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctgga caggtactga cactcaacta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008491
VERSION     KHUT01008491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           96 g           42 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggagcat
       61 caagtcggaa gtgaaatctc gccgctcaac gacgaggctg ctttcgatac tggcgttcta
      121 gggaccggta gaggctggta gaattgcagg tgtagcggtg gaatgcgtag agatctgcaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccgggtccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008492
VERSION     KHUT01008492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttt
       61 caagtcggac gtgaaagccc caggctcaac ctgggaactg cgtttgatac tgttgagcta
      121 gagtgtggaa ggggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccaacactga cgctgatccg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008493
VERSION     KHUT01008493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           86 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttttg
       61 taagtcgcat gtgaaagccc cgggctcaac ctgggaattg catgcgatac tgcatgacta
      121 gagtatgtta gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgaaggca acttcctggg acaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008494
VERSION     KHUT01008494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           87 g           51 t
ORIGIN      
        1 cacgtaggat ccaaacgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtttgg
       61 taagttggat gtgaaatctc cgggctcaac ccggaggtgt cgttcaaaac taccagactt
      121 gaggacggta gaggaaggtg gaattccggg tgtagtagtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctggg ccgttcctga cgctcagacg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008495
VERSION     KHUT01008495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           79 g           54 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agcgtgcgca ggcggttttg
       61 aaagttagaa gtgaaagccc agggcttaac cttggaattg cttttaaaac ttcaagacta
      121 gagtactgga gaggttagcg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccaat ggcgtaggca gctaactgga cagtaactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008496
VERSION     KHUT01008496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           45 c           79 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaagtt
       61 taagtcagat gtgaaatccc ggagctcaac tccggaacag catttgaaac tgatcatcta
      121 gagggtaggc ggagaaaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg attttctagc ttacacctga cgctgatgcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008497
VERSION     KHUT01008497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggctatt
       61 taagctagat gtgaaatccc cgggcttaac ctgggacgtg catttagaac tgggtagcta
      121 gagtacagaa gagggtagtg gaatttcagg tgtagcggtg aaatgcgtag agatctgaag
      181 gaacaccagt ggcgaaggcg actacctggt ctgatactga cactgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008498
VERSION     KHUT01008498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           96 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttgga
       61 tgagtgtggt gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gattcgacta
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008499
VERSION     KHUT01008499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggccgac
       61 taagtcaggt gtgaaatccc ccggctcaac cggggaactg catttgatac tggttggctc
      121 gaggttggta ggggtggatg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccgga ggcgaaagcg atccactggg ccaaatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008500
VERSION     KHUT01008500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           62 c          110 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggaatta ctgggcgtga agcgcgcgca ggcggctgtc
       61 cgcgcccggc gtgaaagccc ccggctcaac cggggagagt cgtcggggac gggacagctt
      121 gagggcggga ggggttggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccaactggc ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008501             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008501
VERSION     KHUT01008501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           46 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggaccgg
       61 caagtttgac gtgaaatctc cactgcttaa cagggagagg ccgttgaata ctaccgatct
      121 ggagtacggc agaggaccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgttactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008502             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008502
VERSION     KHUT01008502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           96 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggttcct
       61 taagtctggt gtgaaaggtt cagggctcaa cccgaagagt gcactggata ctggggaact
      121 tgagtatggg agaggagagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctctctgg cctgtaactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008503
VERSION     KHUT01008503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           76 g           60 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaagcct tcagcttaac tgaagaactg ccattgatac tgatgatctt
      121 gagtatgatt gaggcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaag ccataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008504
VERSION     KHUT01008504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggattga ctgggcgtaa agcgcgtgta ggtggtctcg
       61 ccagtcaggt gtgaaagccc agggctcaac cctggaagtg cacctgatac tacgaggctt
      121 gagttctgta gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caggtactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008505
VERSION     KHUT01008505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           84 g           54 t
ORIGIN      
        1 tacgtaggaa gcaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggaattg
       61 caagtcggtg gtgaaatcct cgtgcttaac acgagagctg ccatcgatac tgcatttctt
      121 gagtgcggga gagagtgatg gaattccagg tgtagcagtg aaatgtgtag atatctggaa
      181 gaacaccagt ggcgaaggca gtcacttggc ccgtaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008506
VERSION     KHUT01008506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           95 g           50 t
ORIGIN      
        1 tacagggggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtttaa
       61 caagtcaggt gtgaaatccc ggggctcaac tccggaagtg catttgaaac tgttagacta
      121 gagtgcagta gaggatggcg gaatacccag tggagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gccatctggg ctgtaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008507
VERSION     KHUT01008507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           98 g           42 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcaagg
       61 caagtcgggt gtgaaagctt ggggcttaac cccgaaaggg cactcgaaac tgccctgcta
      121 gagggccgga gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag agctcgtgag
      181 gaagaccggt ggcgaaggcg gatctttgga cggcccctga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008508             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008508
VERSION     KHUT01008508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           99 g           42 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggcggcgggg
       61 tcagtcgggt gtgaaatctt ggggcttaac cccgaaacgg cactcgaaac tgcccggctg
      121 gagggccgga gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag agatcgtgag
      181 gaagacccgt ggcgaaggcg gatctctgga cggctaactg acgctgaggc gcgaaggcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008509             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008509
VERSION     KHUT01008509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           85 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctctg
       61 taagtcagtg gtgaaatccc gcagctcaac tgtgtgaatt gccattgata ctgtggagct
      121 tgagtacaga cgaggtaggc ggaattgacg gtgtagcggt gaaatgcata gatatcgtca
      181 agaacaccga ttgcgaaggc agcttactag gctgtaactg acgctgaggc acgaaagtgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01008510             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008510
VERSION     KHUT01008510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 caagtcagga gtgaaagccc tgggctcaac ccgggaactg cttttgaaac tgccgagcta
      121 gagtgcggaa ggggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ccgacactga cgctgagtgc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008511
VERSION     KHUT01008511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggtcta
       61 caagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tgtaggtcta
      121 gagtttggca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcggcctggg ccaaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008512
VERSION     KHUT01008512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           57 c           92 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcccgca ggcggtccgc
       61 caagtttgag gcgacaatca ctggcttaac cggtgaacgc cctcaaaaac tggaggactt
      121 gagagcgaga gagggacacg gaactccggg tggagcagtg aaatgcgtag agatccggaa
      181 gaacaccgaa ggcgaaggca gtgtcctggc tcgtttctga cgctgatggg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008513
VERSION     KHUT01008513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           99 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgcgca ggcggcttgg
       61 taagttggct gtgaaagctc ctggcttaac tgggagaggt cggtcaagac tgccaggctt
      121 gaggttggga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008514
VERSION     KHUT01008514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtccgcg gtgaaatcct ggggcttaac cccagaactg ccgttgatac tatctgtctt
      121 gaatattgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg cagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008515
VERSION     KHUT01008515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           85 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggtgacc
       61 taagtcagat gtgaaagccc cgggcttaac ctgggatgtg cgtttgaaac taagtcacta
      121 gagtttgata gaggggggta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca gccccctgga ttaaaactga cgctcaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008516
VERSION     KHUT01008516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgtggga gaggctactg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg ggtagctggc ccaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008517
VERSION     KHUT01008517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtccggg gtgaaagctt ccggcttaac cggaaaaggg ccttggatac tggtggtctg
      121 gagggaggta ggggtctgcg gaactgctgg tggagcggtg aaatgcgttg atatcagcag
      181 gaacgccggt ggcgaaagcg gcggactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008518
VERSION     KHUT01008518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c           97 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcacg
       61 ccagtcggga gtgaaatccc ccggctcaac cggggaacag ctcccgatac tgcgtgcctc
      121 gaggggggta ggggtcgacg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gtcgactgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008519
VERSION     KHUT01008519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtccggg gtgaaatact tcggcttaac cggagaacag ccttggatac tggtggcctc
      121 gagggagata ggggtgcgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008520
VERSION     KHUT01008520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          103 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggtggcttgc
       61 taagaccgat gtgaaatctc cgcgcttacc gtggagaggt cgttggtgac tggtaggctt
      121 gggttcggca gaggtgagtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctcactggg ccgacaccga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008521
VERSION     KHUT01008521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggctggg
       61 taagtctgat gtgaaatctc cgggcccaac ccggaaatgg cattagaaac tacctggctg
      121 gaggcttgga gggggaactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg agttcctgga caagacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008522
VERSION     KHUT01008522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacggagggg actagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggatcgt
       61 taagtcaggg gtgaaatcct ggggctcaac cccagaactg cccttgatac tggcgatctc
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008523
VERSION     KHUT01008523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           86 g           56 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttagat gtgaaatacc ggggcttaac ttcggaattg catttaagac tgccttgctt
      121 gagttctaga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg agtctctggc tagaaactga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008524             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008524
VERSION     KHUT01008524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           48 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaatctc tgggctcaac ccagaggggc cacccgatac tgctgtgact
      121 agagtgtggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc gccactctgg gccataactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008525             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008525
VERSION     KHUT01008525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           37 c           83 g           57 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agagtgcgta agcggttgca
       61 taagtcttgt gtgaaatctt gggactcaat tccagggctg cactagatac tgtgtgacta
      121 gaggatggga gaggtaagta gaattcctgg tggtaagagt aaaatctaag gagatcagga
      181 ggaatgccag aagcgaaagc gacttactag aacatttctg acgttgatgc acgaaagcgt
      241 ggggatcaaa aagg
//
LOCUS       KHUT01008526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008526
VERSION     KHUT01008526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           88 g           57 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcagga gttaaaccat tgggcttaac ccagtgtcgc ttctgatact gcgatgactt
      121 gagtgtggca ggggaagaca gaattccacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaataccgat ggcgaaggca gtcttctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008527
VERSION     KHUT01008527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtctcgt gtgaaagccc tgggctcaac ccaggaagtg cgcgagatac tggcagactg
      121 gagtgacgga gaggatggcg gaatacccgg tgtagaggtg aaattcgtag atatcgggtg
      181 gaacacctgt ggcgaaggcg gccatctgga cgtcaactga cgctgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008528
VERSION     KHUT01008528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           94 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggcccgg
       61 taagtccatc gtgaaagtcc agggctcaac cctggaatgc cggtggaaac tatcgggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008529
VERSION     KHUT01008529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agggtgtgca ggcggggaag
       61 caagtggtgt atgaaatctc ccagcttaac tgggataggt tatgccagac tgcttttcta
      121 gaggacgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg acgccctggc tcgtacctga cgctcagcca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008530
VERSION     KHUT01008530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           81 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggtcaag
       61 aaagtcggtt gtgaaatccc tgggctcaac ccgggaactg cttccgatac ttcatgactc
      121 gaattcggta gagggaggcg gaactccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccaat ggcgaaggca acctcctggg cctgaattga cactgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008531
VERSION     KHUT01008531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           88 g           53 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agcgaacgta ggcggtttct
       61 taagtttggt cttaaagacc ggggctcaac cccgggaatg gatcgaaaac tgggagactg
      121 gaggatgtca gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccagt ggcgaaagcg actttctggg gcattcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01008532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008532
VERSION     KHUT01008532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctgtt gttaaaggcc tgagctcaac tcgggtatgg caatggatac tgggcggcta
      121 gagtgtggta gaggtcacgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg cgtgactggg ccacaactga cgctgaggaa cgaaagccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01008533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008533
VERSION     KHUT01008533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           97 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttgcg
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cgctagaaac tgcgtggctg
      121 gagtgtggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttgctgga ccatcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008534
VERSION     KHUT01008534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           94 g           39 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 caagtgcctt gtgaaatccc acggctcaac cgtggaacgg ctgggcagac tggcggtctt
      121 gggttgccca ggggcagatg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaac ggtgaaaaca gtctgctggg ggcacaccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008535
VERSION     KHUT01008535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccg
       61 tcagtgtctt gtgaaatccc acggctcaac cgtggaacgg ctgggcatac tgcgggtctt
      121 gagacaccta ggggcgagcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtgttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008536
VERSION     KHUT01008536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           99 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtctct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tgggggactt
      121 gagggcagga gaggagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgcacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008537
VERSION     KHUT01008537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           97 g           40 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccggc
       61 caagtcgggg gtgaaagcct gtggctcaac cacagaatgg ccctcgatac tggacggctc
      121 gagaccggaa gaggttggtg gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg gccaactggt ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008538
VERSION     KHUT01008538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          102 g           38 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaagccc acggctcaac cgtggaaggg cctcggatac tgacggcctc
      121 gaggggggta ggggcacgcg gaactgtcgg tggagcggtg aaatgcgttg atatcgacag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008539
VERSION     KHUT01008539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           98 g           42 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ttgggcgtaa agggcgcgta ggcggccttg
       61 ttagtcgggc gtgaaacccc cgggctcaac ccggggactg cgctcgatac ggcaaagctc
      121 gagggtggaa gagggccgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcggcctggt ccatttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008540
VERSION     KHUT01008540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          106 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggacgct
       61 taagtgtggc ctgaaagccc ccggctcaac cggggagggc ggctgcaaac tgggcgtctt
      121 gagggcgata gaggtcggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccgactggg tcggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008541
VERSION     KHUT01008541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          105 g           40 t
ORIGIN      
        1 tacgtggggg gcgagcgttg tccggaatta ctgggcgtaa agcgcctgta ggcggggcgg
       61 gaagtcggac gtgaaaggct ggggctcaac cccagaaggc cgttcgaaac tctcgctctt
      121 gagggtcgaa gaggagcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcgctctggt cggctactga cgctgagagg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008542
VERSION     KHUT01008542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggtttta
       61 caagtctgct gtcaaagcgc gaggcttaac ctcgtacagg cggtggaaac tgtgagacta
      121 gagtatggta ggggtagcgg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagc ggcgaaagcg cgctactggg ccattactga cactcatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01008543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008543
VERSION     KHUT01008543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           78 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatggg
       61 taagtccgtg gtgaaatctc caagcttaac ttggaaactg ccatggatac tatcgatctt
      121 gaattctgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca cagcgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008544
VERSION     KHUT01008544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          103 g           40 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgccg
       61 cgagtcactt gtgaaatctc cgggcttaac ccggagcggc caagtgatac tgcggcgctc
      121 gagtgcggaa ggggccactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggtggctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008545
VERSION     KHUT01008545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           85 g           55 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtcttgt
       61 taagtcttcg gtgaaagccc ccggctcaac tggggaattg tcggagatac tggcaagatt
      121 gagtgtgaca gaggcagacg gaattcctgg tgtagcggta aaatgcgttg atatcaggag
      181 gaacaccaat ggcgaaggca gtctgctggg tcattactga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008546
VERSION     KHUT01008546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcataa agagcatgta ggtggtccgc
       61 taagtcacgt gtgaaatccc ccggctcaac cggggaatgg cgcgtgatac tggcggactc
      121 gaggcaggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga cctgatctga cactgagatg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008547
VERSION     KHUT01008547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           84 g           50 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtctttt
       61 taagtccgcg gtgaaatctc cgggcccaac ccggaaattg tcgcggaaac tggaaggatt
      121 gagtatcgca gaggcagacg gaatgcttgg tgtagcggta aaatgcgtag atatcaagca
      181 gaacaccgat ggcgaaggca gtctgctggg cgattactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008548
VERSION     KHUT01008548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c          102 g           35 t
ORIGIN      
        1 gacagagggg gccagcgttg tccggaatga ctgggcgtac agcgcgcgca ggcggagatg
       61 gacgtgccgt gtgacagcta tcggctcaac cgatagtcgc cacggtagac gccatatctg
      121 gagtgggtga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagt ggcgcaagcg gcatcctggc accaaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008549
VERSION     KHUT01008549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           63 c          114 g           28 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatga ctgggcgtaa agggcgcgca ggcggtcagg
       61 cgcgtcggtc gtgaaagccc ccggctcaac cggggcgggt cgaccgagac ggcgtgactg
      121 gaggctggca gaggggggtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccaggactga cgctgaggcg cgacggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008550
VERSION     KHUT01008550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          102 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggagcgt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcgtac tggcgctctt
      121 gaggacgaca gaggagtgcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccgat ggcgaaggca gcactctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008551
VERSION     KHUT01008551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          100 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcggct gtgaaaaccc gcagcttaac tgcgggcctg cagtcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008552
VERSION     KHUT01008552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           99 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggctcgg
       61 taagtccgcc gtgaaaaccc ggggctcaac cccggacttg cggtggatac tgtcgggcta
      121 gaggatggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccattcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008553
VERSION     KHUT01008553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           98 g           50 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatta ctgggcttaa agggcgcgta ggtggtgata
       61 taagtccggg gtggaagact ggggcttaac ctcagaattg ccttggatac tgtatgactt
      121 gagtcagcga ggggatggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaaggccggt ggcgaaggcg gccatctggc gctgaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008554
VERSION     KHUT01008554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          102 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgtgta ggcggctggg
       61 taagccgcgt gtgaaatccc ggggctcaac cccggaacgg cacgcgggac tgtccggcta
      121 gagaatggta gaggcgagtg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008555
VERSION     KHUT01008555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggtcatt
       61 taagtctgat gtgaaagccc tgggcttaac ctgggaatgt cgttggaaac tgagtgacta
      121 gagtcggaca gagggccgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacacccgt ggcgaaggcg gcggcctggg tccagactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008556
VERSION     KHUT01008556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           44 c           79 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agggcgcgta ggcgggatag
       61 caagtcagat gtgaaatcct ggagcttaac tccagaacag catctgaaac tacaattcta
      121 gaggatagac ggggaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagt ttattcctga cgctaaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008557
VERSION     KHUT01008557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacggagggg gcaaacgttg ttcggaatta ctgggcgtaa agggtgagta ggcggttcga
       61 ttagtctagc gtgaaatctc tcggcttacc cgggaaactg cgctggaaac tgtcgagctc
      121 gaggatggaa gaggcagatg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacactcgt ggcgaaggcg gtctgctggt ccatttctaa cgctgaatca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008558
VERSION     KHUT01008558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtccat
       61 caagtcagag gtgaaagccc ggggctcaac cccggaactg cctttgaaac tggcggacta
      121 gaatcttgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008559
VERSION     KHUT01008559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcacgca ggcggcaaag
       61 taagtcagtt gtgaaactcc cgggcttaac ttgggaactg cagctgatac tgccttgctt
      121 gagtacggaa gaggggagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctccctggt ccgatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008560
VERSION     KHUT01008560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca gacggacgat
       61 taagtggtgt atgaaagcac caggctcaac ctggtgtcgc tatgccagac tggtcgtctt
      121 gaggatggga gagggacgcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc ccatatctga cgttcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008561
VERSION     KHUT01008561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           84 g           59 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccggaggg
       61 taagcgtgtt gtgaaatgca gttgctcaac atctgcactg cagcgcgaac tgttctcctt
      121 gagtgcgcag gaagtaggcg gaattcgtcg tgtagcggtg aaatgcttag atatgacgaa
      181 gaactccgat tgggaagcca gcttactgta gcgccactga cgctgatgct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01008562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008562
VERSION     KHUT01008562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           73 g           68 t
ORIGIN      
        1 tacgtaggac gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaaaaa
       61 taagtcagtg gtgaaatatt gtcgcttaac gataagattg ccattgatac tgtttttctt
      121 gagtacaatt gaggtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat tgcgaaggca gcttactaaa ttgttactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008563
VERSION     KHUT01008563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           76 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggactga
       61 taagtcagcg gtgaaatccc acggctcaac tgtggaactg ccgttgatac tgttagtctt
      121 gaatgcagat gatgtgggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtgattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008564
VERSION     KHUT01008564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           93 g           49 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttacg
       61 tcagtcggat gtgaaagccc cgggcttaac ctgggaattg catccgatac tgcgtagcta
      121 gagtctggta gagggtagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gctacctgga ccaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008565
VERSION     KHUT01008565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           86 g           49 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggtccgg
       61 taagtcggat gtgaaatctc gaggcctaac ctcgattggt cattcgaaac taccgggcta
      121 gaggattgga gaggagactg gaatagttgg tgtagcagtg aaatgcgtag agatcaacta
      181 gaacaccggt ggcgaaggcg agtctctgga caattcctga cgctcaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008566
VERSION     KHUT01008566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           93 g           51 t
ORIGIN      
        1 gacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcacgta ggtggatgtt
       61 taagatgagt gtaaaagacc tgggcttaac ctgggggggt cactcaagac tgggcatcta
      121 gagtacgata gaggggagtg gaatttccgg agtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actttctgga ttgatactga cactgaggtg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008567
VERSION     KHUT01008567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcttgc
       61 ttagtcagac gtgaaaatcc cgggcttaac ctggggactg cgtttgatac gggcaggctt
      121 gaggatggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008568
VERSION     KHUT01008568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggttcgt
       61 acagtcagac gtgaaattcc cgggctcaac ctgggggctg cgtttgagac gtacgggctt
      121 gagtccggaa gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccacctggt ccggcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008569
VERSION     KHUT01008569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           93 g           43 t
ORIGIN      
        1 gacgaaggtg acgaccgttg ctcggattca ctgggcataa agcgcgcgta ggtggctagg
       61 caagtccatg gtgaaatccc tcggctcaac cgaggaaggg ccgtggatac tgcctggctt
      121 gaggtcggga ggggtaagtg gaactctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacgccggt ggcgaaagcg acttactgga ccgtacctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008570
VERSION     KHUT01008570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           37 c           84 g           55 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggtgtaa agggtgagta ggcggttaga
       61 taagccatat gtgaaagtct gtagctcaac tatagaattg cataaggaac tgtttgacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgaaactga cgctgagtca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008571
VERSION     KHUT01008571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           46 c           80 g           50 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggaatca ctgggcgtaa agcgttcgta ggggggaatg
       61 taagtctcat attaaatccc gatgctcaac atcggactgg taggggaaac tgcatttcta
      121 gaggatggga gaggctagtg gaattctcgg tgtaggggta aaatccgtag atatcgagag
      181 gaacaccaaa agcgaaggca gctagctgga acattcctga ccctcaagaa cgaaagcgtg
      241 gggagcgaaa agg
//
LOCUS       KHUT01008572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008572
VERSION     KHUT01008572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           85 g           55 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttaat
       61 taagttagat gtgaaagccc cgggctcaac ctgggaattg catttaatac tgattaacta
      121 gagttcgata gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccaga ggcgaaggcg gctttctgga tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008573
VERSION     KHUT01008573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          102 g           38 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtctcg
       61 aacgtggtgc gtgaaagcgc ccggctcaac cgggcgagga cgggccaaac ggcgggactt
      121 gagggaggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg acacgctggg ccttctctga cgctcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008574
VERSION     KHUT01008574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           85 g           59 t
ORIGIN      
        1 gacagaggtg tcaagcgttg ctcggaatta ctgggcgtaa agcgtgtttc ggtggtttgt
       61 taagtcatgc gtgaaagctc ttggcctaac caagaaatcg cgtgtgatac tggctaacta
      121 gagggtggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gctctctggt ccatttctga cactttcaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008575
VERSION     KHUT01008575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           75 g           66 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggtttg
       61 taagtcagtg gtgaaagact gtcgcttaac gatagcattg ccattgatac tgcagatctt
      121 gagtatactt gaggtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gcttactaaa ttataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008576
VERSION     KHUT01008576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           85 g           51 t
ORIGIN      
        1 gacagaggtc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcctg
       61 caagtacttt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgctggtctt
      121 gaggcatcta gaggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgcccat ggtgaaaaca ggttgctggg gatgtactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008577
VERSION     KHUT01008577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaaga
       61 taagtcagat gtgaaatcct ggggctcaac ctcagaactg catttgaaac tatctttcta
      121 gagggtagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01008578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008578
VERSION     KHUT01008578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggggtga
       61 taagtggtgt atgaaatctc tgggcttaac tcggaggggt tatgccagac tgtcactcta
      121 gagtgcgaga gaggaacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgttctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008579
VERSION     KHUT01008579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           97 g           41 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggatctg
       61 cgcgtggggt gtgaaatcga acggcttaac cgttcgccgc catcccatac ggcaggtctg
      121 gagccatgca gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgactggg catgtgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008580
VERSION     KHUT01008580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcgaca
       61 taagttggag gtgaaatccc cgggctcaac ctgggaactg ctttcaaaac tgtgtggcta
      121 gagtatggta gaggtgagtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacatcagt ggcgaaggcg actcactgga ctaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008581
VERSION     KHUT01008581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggattca ctgggtttaa agggtgcgta ggtggggatg
       61 taagtcagtg gtgaaatctc cgtgcttaac acggaaactg ccattgatac tatgtttctt
      121 gaattatctg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca ggtggattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008582
VERSION     KHUT01008582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           76 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaatag
       61 taagtcagtg gtgaaagccc gcagcttaac tgcggaattg ccattgatac tgctaatctt
      121 gaatgtagat gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctaatattga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008583
VERSION     KHUT01008583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtctgg
       61 taagtcggcg gtgaaagccc ggggctcaac cccggaactg ccatcgatac tgtcagactt
      121 gagtgtggtt gagggtggcg gaatgggcgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctgcctaag ccacaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008584
VERSION     KHUT01008584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttct
       61 taagtctgtt gtgaaagccc tgggctcaac ctgggaactg cagtggatac tgagaagcta
      121 gagtgtgtca gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcagt ggcgaaggcg gccatctggg acaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008585
VERSION     KHUT01008585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           45 c           81 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgtgta ggcggaaatg
       61 taagtcagat gtgaaatccc ggagctcaac tccggaacag catttgaaac tgcacttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008586
VERSION     KHUT01008586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           65 c           97 g           33 t
ORIGIN      
        1 gacagaggag gcaagcgttg tccggactga ctgggcgtaa agcgtccgca ggtggtcgtg
       61 caagtgcgct gtgaaagcgg cccgctcaac gggccgaggc cagcacaaac tgctcgactc
      121 gaggtcggga gaggtcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca gccgactggc ccgcacctga cactcaggga cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008587
VERSION     KHUT01008587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           85 g           58 t
ORIGIN      
        1 tacgtaggga gcaagcgtta tccggaatta ctgggtgtaa agggagtgta ggcgggatta
       61 caagtcagat gtgaaaactg tgggctcaac tcacagattg catttgaaac tgtagttctt
      121 gagtgaagta gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacatcagt ggcgaaggcg gcttactggg ctttaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008588
VERSION     KHUT01008588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatg
       61 caagacagat gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcgtgacta
      121 gagtatggta gagggggatg gaattccgca tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca atcccctgga cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008589
VERSION     KHUT01008589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttt
       61 taagtcagag gtgaaagccc ggggctcaac tccggaatag cctttgagac tggatggctt
      121 gaatcacgga gaggtgggtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcccactgga cgtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008590
VERSION     KHUT01008590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcggatctt
       61 taagtccgtg gtgaaatctc caggctcaac ctggaaactg ccatggatac tattggtctt
      121 gaatcttgtt gaggtgggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaag caagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008591
VERSION     KHUT01008591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           84 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ctgggcgtaa agagttcgta ggcggtctat
       61 taagtctgtt gtgaaagccc tggacctaat ccaggaagtg catcggatac tgatagacta
      121 gaatgcagga ggggagagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaagcg gctctctgga ctgctattga cgctgaggaa cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008592             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008592
VERSION     KHUT01008592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaaactc caggctcaac ctggagatgc cacccgatac tgctatgact
      121 tgagtctggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008593
VERSION     KHUT01008593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          103 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgta ggcggaagtt
       61 taagtgtggc ctgaaagccc ccggctcaac cggggagggt ggctgcaaac tgggcttctt
      121 gagggggata gaggagagtg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgtaggcg gctctctggg tccgccctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008594             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008594
VERSION     KHUT01008594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           79 g           56 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ctgggcgtaa agtgtgcgta ggtggttgaa
       61 taagttactg gtggcaaatc ggggctcaac tccgtggaag ctagtaatac tattcaacta
      121 gagggccgga gaggttaacg gaattctatg tgtagcggta aaatgctcaa atatatagag
      181 gaacaccaat cggcgaaggc agttaactat tcggctcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01008595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008595
VERSION     KHUT01008595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           50 c           80 g           50 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtatgca ggcggccatg
       61 caagtcagaa gtgaaatccc tgggcttaac ccaggaattg cttttgaaac tgcacggcta
      121 gaatcccgga gaggttagta gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgcaagcg actaactgga cgggcattga cgctcagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008596
VERSION     KHUT01008596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           55 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgtcg
       61 taagtcaggt gtgaaatctc ggggctcaac cccgaaactg cacttgatac tgcgatgctt
      121 gagtattgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga caattactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008597
VERSION     KHUT01008597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           86 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttgg
       61 caagttggat gtgaaagccc cgggcttaac ctgggaactg cattcaatac taccaagcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcttcctgga ctcatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008598
VERSION     KHUT01008598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggttgg
       61 gaagtcagtg gtgaaatact ctcgcttaac gggagaactg ccgttgatac tatcaatctt
      121 gaatatagtg gaggtgagcg gaatgtgtca tgtagtggtg aaatacttag atatgacaca
      181 gaacaccaat tgcgaaggca gcttgctaca ctattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008599
VERSION     KHUT01008599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           87 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggatat
       61 taagtcagtg ttcaaaaacg gcagctcaac tgtcggccgg gcattgatac tggtattctt
      121 gagaccattg ggggcagccg gaatttgtgg tgtagcggtg aaatgcttag ataccacaag
      181 gaacaccgat cgcgaaggca ggctgccacg ctggatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008600
VERSION     KHUT01008600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           36 c           90 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggtgtgta ggtggtttaa
       61 taagttaggt gtgaaagtcc tgggcttaac ctggggatgg cgcttaagac tgttaggcta
      121 gagtactgta gaggaaagtg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008601
VERSION     KHUT01008601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           78 g           56 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgta ggcggcatac
       61 caagtcagtt gttaaattct tcggcctaac cgaagtccag caattgaaac tagtatactt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgctgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008602
VERSION     KHUT01008602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctctg
       61 caagtcggat gtgaaagccc tgggctcaac ctaggaagtg cactcgaaac tgcagagctg
      121 gaggtctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagcacctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008603
VERSION     KHUT01008603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcgggacac
       61 taagtctgat gtgaaatccc tcggcttaac tgaggaactg cattggatac tggtgacctt
      121 gagtcctgga gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cagtgactga cgctgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008604
VERSION     KHUT01008604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtctg
       61 taagtcagaa gtgaaattgc actgctcaac ggtgtagctg cttctgatac tgcagatctt
      121 gagaccggta gaggttggtg gaattgcagg tgtagcggtg gaatgcgtag agatctgcaa
      181 gaacacccgt ggcgaaggcg gccaactggg ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008605
VERSION     KHUT01008605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           88 g           55 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttag
       61 taagttggaa gtgaaagccc gaggcttaac ctcggaattg ctttcaaaac tgctaagctt
      121 gagtgtagta ggggatgatg gaatttctag tgtagaggtg gaattcttag atattagaag
      181 gaacaccggt ggcgaaagcg atcatctggg ctacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008606
VERSION     KHUT01008606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggattgt
       61 taagtcaggg gtgaaatgcc gaggctcaac ctcggaactg cctttgatac tggcaatctt
      121 gagtccggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008607
VERSION     KHUT01008607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           62 c          109 g           32 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggccggg
       61 aaggtgccgt gtgacaggcg caggcttaac ctgcgacggc cacggtagac cgcccggctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgcaggcg gcatcctggc cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01008608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008608
VERSION     KHUT01008608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           83 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcgggcctt
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tggaggtctt
      121 gagtgccgga gaggaagatg gaactacacg tgtagcggtg aaatgcgttg agatgtgtag
      181 gaacaccaat ggcgaaagca atcttctgga cggatactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01008609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008609
VERSION     KHUT01008609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           38 c           90 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgaa
       61 tgagttgggt gtgaaacccc tgggcttaac ctaggaagtg cattcaatac tgtttagcta
      121 gagtatggta gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaggcg gcttactgga ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008610
VERSION     KHUT01008610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           92 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatca ttgggcgtaa agcgtacgca ggcggccttc
       61 caagtcgggt gtgaaagccc agggctcaac cctggaactg cgcccgagac tgttaggctt
      121 gagtgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008611
VERSION     KHUT01008611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctgtt gtgaaagctc ctggcttaac tgggagaggt caacggaaac tgccgggctt
      121 gagtgtggta ggggaagcta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctagg ccaccactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008612
VERSION     KHUT01008612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctt ggggctcaac cccgaaactg ctttacatac tggcagactg
      121 gagggatgca gaggtttgtg gaattcctgg tgtagcggtg aaatgcattg atatcaggag
      181 gaacacccat ggcgaaggca gcaaactggg catcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008613
VERSION     KHUT01008613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ttgggcgtaa agggcgcgta ggcggttaga
       61 caagtgagat gtgaaagccc tgggcttaac ctaggaattg catcttaaac tgtatagcta
      121 gagagcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008614
VERSION     KHUT01008614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           98 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttgga
       61 caagtggtgt gtgaaagctc ccggctaaac tgggagagga cacatcagac tgttcggcta
      121 gaggatgcta gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggtgaagacg gcgccctggg gcattactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008615
VERSION     KHUT01008615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacgtatgga gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggtttgg
       61 caagtctgaa gtgaaagccc ggggctcaac ctcgggactg ctttggaaac tgtcgaacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008616
VERSION     KHUT01008616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           99 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggccgtg
       61 caagtccctg gtgaaagctc gatgctcaac atcgagtcgg ccagagatac tgcagggctg
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008617
VERSION     KHUT01008617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccctc
       61 tcagtcggat gtgaaatccc caggcttaac ctgggaacgg catccgatac tggagggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgagattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008618
VERSION     KHUT01008618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaataa ctgggcttaa agggtgcgta ggcgggctgt
       61 caagtctggg gtgaaatccg gcagcttaac tgtcgaactg ccttagatac tgatggcctc
      121 gaggaaggta ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaagcg gcgtgctgga ccttttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008619
VERSION     KHUT01008619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           62 c          108 g           31 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggagtga ctgggcgtac agcgcgcgca ggcgggacgc
       61 gacgtgccgt gtaacagcgc actgctcacc agtgcgtagc catggtagac gcgcggcctg
      121 gaggccgtga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgcaggcg gcatcctggc acggacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008620
VERSION     KHUT01008620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           96 g           45 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggcgcct
       61 taagttcggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tggggcactg
      121 gagcgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccagc ggtgtagacg gctttctgga ccatggctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008621
VERSION     KHUT01008621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaattg cctttgaaac tgagaagcta
      121 gagtactgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagtaactga cgctctggcg cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008622
VERSION     KHUT01008622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cacgtcggtt gtgaaaaccc aaggctcaac cttgggcctg caatcgatac gggcagacta
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtaactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008623
VERSION     KHUT01008623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           95 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agagcccgca ggcggtctgt
       61 taagtttcag gtgaaatctc ctggctcaac tgggaggggt cctggaaaac tggcagactt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008624
VERSION     KHUT01008624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatg
       61 taagttagat gtgaaatccc tgggcttaac ctgggcatgg catttaagac tgcaaagcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008625
VERSION     KHUT01008625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatcct gcagcttaac tgtagaaggg cctttgatac tggcaagctc
      121 gagggaggta ggggtatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008626
VERSION     KHUT01008626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcggtg gtgaaatcct gcagcttaac tgtagaagtg cctccgatac tggcaggctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tctcatctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008627
VERSION     KHUT01008627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c          100 g           36 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 caagtcggaa gtgaaagccc ggggctcaac cccggaactg cttcggaaac tgccgagcta
      121 gagtgcggaa ggggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccgga ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008628
VERSION     KHUT01008628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgt
       61 caagtcagga gtgaaatcct tcggctcaac cggagaagcg cttttgatac tggcgacctc
      121 gaggggtgca ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008629
VERSION     KHUT01008629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttagt
       61 taagtcgggt gtgaaatccc taggctcaac caaggaagtg catccgaaac taactagcta
      121 gaagacagta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctggg ctgttcttga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008630
VERSION     KHUT01008630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggagcg
       61 cgcgtctggg aggaaatctc acggctcaac cgtgtggctt tcccagatac ggcgcttctt
      121 gagggcagga gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agttactggc ctgcatctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008631
VERSION     KHUT01008631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           64 c          107 g           34 t
ORIGIN      
        1 gacagaggag gccagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggatgtg
       61 ggcgtcggtg ctgaaaggcc cccgcttaac gggggacggc gtgccgatac gccatgtctg
      121 gagggcgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt ggcgcaggcg accacctagc ccgcacctga cgctgaggcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008632
VERSION     KHUT01008632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c          101 g           34 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggcgccg
       61 cacgtcgggt gtgaaagccc ggggctcaac cccggaagcg cgcacgaaac gacggcgctg
      121 gaggcctgga gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctgga caggacctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008633
VERSION     KHUT01008633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaagctc gcggctcaac tgcgaaatgg catcggaaac tatccggcta
      121 gagggatgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccttaga catctcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008634
VERSION     KHUT01008634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctac
       61 taagtctggt gtgaaatccc cccgctcaac gggggaacgg cgccggatac tggtagactc
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008635
VERSION     KHUT01008635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           56 c          106 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggcccgg
       61 cgcgtcggct gtgaaatctc ccggctcaac tgggagggga cggccgatac ggctgggctt
      121 gagcgaggta ggggagagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cctctgctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01008636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008636
VERSION     KHUT01008636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcatca
       61 caagtcagaa gtgaaaatcc ggggctcaac cccggaactg cttttgaaac tgtggagctg
      121 gagtgcagga gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008637             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008637
VERSION     KHUT01008637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgtta ttcggattta ttgggcgtaa agggcgtgta ggcggcttga
       61 aaagtctggt gtgaaaacgc taccgctcaa cggagcgaat gcgctggata ctttcaaact
      121 tgagttcgag agaggcgggc agaatacctg gtgtagcggt ggaatgctta gagatcaggt
      181 agaatacccg aggcgaaggc ggcccgctgg ctcgacactg acgctgaggc gcgaaagcgt
      241 ggggatcaaa cggg
//
LOCUS       KHUT01008638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008638
VERSION     KHUT01008638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           91 g           56 t
ORIGIN      
        1 tacgtaggac ccgagcgttg tccggattta ctgggtgtaa agggtgcgta ggcggatagg
       61 tgcgtcagag gtgaaatttc ccggcttaac tgggagggtg cctttgatac ggcttatctt
      121 gagtgcgaaa gaggatgatg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gtcatctggt tcgcaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008639
VERSION     KHUT01008639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 cacgtaaggg gcgagcgttg ttcggaatta ttgggcgtaa agggcacgca ggcggttata
       61 caagtccgat gtgaaagacc tgggcttaac tcaggggccg cgttggaaac tgtatgacta
      121 gagtattaga gggggagtcg gaattcctag tgtaggggtg aaatctgttg atattaggaa
      181 gaacaccggt ggcgaaggcg gactcctggc tggatactga cgctgaggtg cgaaagcata
      241 gggagcaaac agg
//
LOCUS       KHUT01008640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008640
VERSION     KHUT01008640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 aaagtcaggg gtgaaatcct ccagctcaac tggagaactg cctttgatac tggcggcctt
      121 gagtggggta ggggcatgcg gaacaatggg tggagcggtg aaatgcgttg atatccattg
      181 gaactccggt ggcgaaagcg gcgtgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008641             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008641
VERSION     KHUT01008641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           43 c           77 g           54 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggttgaa
       61 taagttagca gcggcaaatt gaggctcaac ctcacggaag ttgctaatac tattcaactt
      121 gagggcagaa gaggttaacg gaactctatg tggaggggtg aaatccaacg atatatagat
      181 gaacaccaaa aggcgaaggc agttaactat tctgctcctg acattgaggc acgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01008642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008642
VERSION     KHUT01008642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           77 g           55 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggatag
       61 taagtcagtg gtgaaagccc gcagctcaac tgcggaactg ccattgatac tgctaacctt
      121 gagtatgtat gatgtgggcg gaatgtgcag tgtagcggtg aaatgcttag atattgcaca
      181 gaacaccaat tgcgaaggca gctcacaaaa acataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008643
VERSION     KHUT01008643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggcttgt
       61 caggtccgat gtgaaagccc cgggctcaac ccgggaagtg cattggaaac cgacaggctt
      121 gaatacggga gagggcagtg gaattcctgg tgtaggagtg aaatccgcag atatcaggag
      181 gaacaccggc ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008644
VERSION     KHUT01008644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           96 g           42 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggtccca
       61 aaagtctgat gtgaaagccc ggggctcaac cccggaagtg cattggaaac tgtgggactt
      121 gagttcggga gaggaaggca gaattcccgg tgtagggttg aaatccgtag atatcgggag
      181 gaaaaccggt ggcgaaggcg gccttctgga acgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008645
VERSION     KHUT01008645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttcg
       61 caagtcagat gtgaaagccc cgggcttaac ctgggaactg cgtttgaaac tacgaagcta
      121 gagtacggca gagggaggtg gaattccaag tgtagcagtg aaatgcgtag atatttggag
      181 gaacaccgat ggcgaaggca gcctcctggg ctagtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008646             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008646
VERSION     KHUT01008646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           99 g           48 t
ORIGIN      
        1 tacggggggg ggcaagcgtt gttcggaatt actgggcgta aagggcgcgt aggcggttgg
       61 ataagtcagc tgtgaaaacc acgggcttaa ctcgtggcct gcagttgaga ctgtctggct
      121 tgagtgtggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggctctctgg accacaactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008647
VERSION     KHUT01008647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtttgt
       61 taagtcatgt gtgaaatccc tcggctcaac cggggagcga cgcatgaaac tggcaagcta
      121 gagtaccaaa gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctggt tggatactga cgctgatacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008648             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008648
VERSION     KHUT01008648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggcccgt
       61 taagtgagat gcgaaatctt gtggctcaac catatagatg ttgtctcata ctggcgagct
      121 tgaggacggc agaggcaagt ggaattacta gtgtagcagt gaaatgcgta gatattagta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatttctg acgctgaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008649
VERSION     KHUT01008649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgtgca ggcgggcgga
       61 taagtggtgt atgaaatatc ccggctcaac cgggaagggt tatgccagac tgtccgtctt
      121 gagtgcgaga gaggagcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgctctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008650
VERSION     KHUT01008650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggattca ctgggcgtaa agagcgcgta ggcggtttgg
       61 caagtctgat gtgaaagccc tcgacttaat cgaggaagtg cattggatac tgccagacta
      121 gagtatagga gaggaagatg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaacaga ggcgaaggcg atcttctgga ctattactga cgctgaggtg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008651
VERSION     KHUT01008651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggcatgg
       61 taagtttggt gtgaaagctt cgggcttaac ccggaaatgg cattgaaaac tactgtgctg
      121 gaggatcgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgatacctga cactgaggca cgaaagccaa
      241 gggagcaaac agg
//
LOCUS       KHUT01008652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008652
VERSION     KHUT01008652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggctgtt
       61 acagtcaggc gtgaaattcc tgggctcaac ctgggggctg cgcttgatac gtgatggcta
      121 gaggatggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008653
VERSION     KHUT01008653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           78 g           61 t
ORIGIN      
        1 tacgaagggt gctagcgtta ctcggaatta ctgggcgtaa agaatgcgta ggcggactta
       61 taagttggga gtgaaatccc cgggctcaac ccgggtgctg ctttcaaaac tgtaagtctt
      121 gaattttgga gtgattaggg gaattcttgg tgtaggggta atatccatag atatcaagag
      181 gaagaccgga agtgaaaacg cttaattggc caaatattga cgctgatgca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008654
VERSION     KHUT01008654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           79 g           58 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcccgg
       61 taagtcagtg gtgaaatctc cccgctcaac ggggaaactg ccattgatac tgccgggctt
      121 gaattactgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccgat tgcgaaggca ggttactacc attatattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008655
VERSION     KHUT01008655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcggg
       61 taagtcggat gtgaaatcct ggggctcaac ctcagaactg cattcgatac tgcctggcta
      121 gaggattgga gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag agatcgtgag
      181 gaagaccaac ggcgaaggca gctctctgga caattcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008656
VERSION     KHUT01008656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           76 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggttttg
       61 taagtcagtg gtgaaagccc ggagctcaac tccggaactg ccattgatac tgcttaactt
      121 gaatcaactt gaggtggatg gaatattaca tgtagcggtg aaatgcttag atatgtaata
      181 gaacaccgat tgcgaaggca gttcactaag gttgtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008657
VERSION     KHUT01008657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           55 t
ORIGIN      
        1 tacgaagggg gcgagcattg ttcggaatta ctgggcgtaa agggtgcgta ggcggattgg
       61 atagtcagat gtgaaagccc aaggctcaac cttggaattg catttgatac tgccagtctg
      121 gagtattgga gaggaacgtg gaattctcag tgtagaggtg aaattcgtag atattgagag
      181 gaacaccagt ggcgtaggcg acgttctggc caaatactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008658
VERSION     KHUT01008658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          107 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttgga
       61 gaaggctgtg gtgaaagtcc ggggctcaac cccggatcgg ccgtggcgac tctctgactg
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008659
VERSION     KHUT01008659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 tacgtagggg acgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggctact
       61 caagtcctgc gtgaaatctc gaggctcaac ctcgagcggt cgtgggaaac tgggtggcta
      121 gagtgcggga gaggagagtg gaattcccag tgtagcggtg aaatgcgcag atattgggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008660             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008660
VERSION     KHUT01008660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           89 g           57 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agggcgcgta ggcggttaag
       61 taagtcgggt gttaaatctc caagctcaac ttggagctgc cattcgaaac tgctttgact
      121 tgagtctggt aggggaacac ggaatacctg gtgtagcggt gaaatgctta gatatcaggt
      181 ggaacaccag tggcgaaggc ggtgttctgg gccagtactg acgctgatgc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008661             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008661
VERSION     KHUT01008661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcggat gtgaaatctc caggctcaac ctggaggggt cattcgatac tgccatgact
      121 cgagtccggt aggggagtgc ggaattcctg gtggagcggt gaaatgcgca gatatcagga
      181 ggaacaccca tagcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008662
VERSION     KHUT01008662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggtttga
       61 ttagttcgct gtgaaagtcg ggggctcaac cctcgaatgc cggtgaatac tgtcagacta
      121 gagtgcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008663
VERSION     KHUT01008663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacgaaggtt gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 taagtctgtt gtgaaagctc ctggcttaac tgggagaggt caatagatac tgctgaactt
      121 gagtatagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ctattactga cgctaatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008664
VERSION     KHUT01008664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctgtc
       61 taagtagggt gtgaaagccc ccggctcaac cggggaattg cgccctaaac tggacggctg
      121 gagtgaggca ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008665
VERSION     KHUT01008665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcacgta ggcggtcgta
       61 taagtcagtt gtgaaatccc cgggcttaac ctgggaactg cagctgatac tgtatgactc
      121 gagtcgaaga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatctgt ggcgaaggcg gcttcctggc ttcagactga cgctgaggtg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008666
VERSION     KHUT01008666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttata
       61 taagacagat gtgaaatccc cgggcttaac ctgggaactg cgtttgtgac tgtatagcta
      121 gagtacggca gaggggggtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatcgat ggcgaaggca gccccctggg cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008667
VERSION     KHUT01008667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc ccggctcaac cggggaactg ctgggtatac tggcgatctt
      121 gagcgatcta ggggcagatg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaaa ggtgaaaaca gtctgctggg gatctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008668             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008668
VERSION     KHUT01008668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           82 g           63 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggtttaa
       61 tcagtcggtg atgaaatgac ggggcttaac cttagtcgct gttaccgata ctgttagact
      121 agagatagag taaggcagct ggaataagtg gtgtagcggt gaaatgcgta gagatcactt
      181 agaacaccga ttgcgaaggc aggttgctgg ctcttatctg acgctgatgc acgaaggcat
      241 ggggatcaaa cagg
//
LOCUS       KHUT01008669             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008669
VERSION     KHUT01008669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcgggt gtgaaaaccc caggctcaac ctggggacgc cacctgatac tgctatgact
      121 tgagtctggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccagtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008670             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008670
VERSION     KHUT01008670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta gacggacgac
       61 cgcgtcgacg gctgaaagcc cccggctcaa ccggggaacg ggcctcgata cgggtcgtct
      121 cgagtggagg agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg actccgactg acgttgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008671
VERSION     KHUT01008671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          107 g           33 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcacgc
       61 cacgcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac gggcgagctt
      121 gagcgcacca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtgtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008672
VERSION     KHUT01008672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          102 g           45 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtttag
       61 caagtccggt gtgaaagctc ccggctcaac tgggagaggt cattggagac tgctgaactg
      121 gaggatggca gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ccatacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008673
VERSION     KHUT01008673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           83 g           54 t
ORIGIN      
        1 tacggagggt gcaaacgtta tccggaatca ctgggcgtaa agcgcgtgta ggtggtttag
       61 taagtgggcg attaaagacc agtgcctaac actgggactg ttacctatac tgctagactt
      121 gaggaagata ggggcaagtg gaactttgag tgtaggagtg aaatccgtag atattcaaag
      181 gaacaccaat ggcgaaggca gcttgctgga tcttatctga cactgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008674
VERSION     KHUT01008674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           87 g           59 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agagttcgta ggtggcctgt
       61 taagtctggt gttaaatgca gatgctcaac atctgttcgg cactggatac tggcaagctt
      121 gaatgcggta gaggtaaagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcggt ggcgtaagcg ctttactggg ccgtaattga cactgaggaa cgaaagccag
      241 ggtagcaaat ggg
//
LOCUS       KHUT01008675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008675
VERSION     KHUT01008675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           80 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggacttt
       61 taagtagaaa gtgaaaggtt ggagctcaac tccgacactg cttcctatac tgggagtctt
      121 gaatgccgga gaggaagatg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca atcttctgga cggatattga cgctcatgca cgaaagctaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01008676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008676
VERSION     KHUT01008676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           88 g           54 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggttatg
       61 caagtccggt gtgaaatccc acagctcaac tgtggaactg cattggaaac tgcatgactt
      121 gagtactgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cggttactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008677
VERSION     KHUT01008677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctgc
       61 caagctcgat gtgaaagccc cgggcttaac ctgggaattg cattgaggac tgacaggctc
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008678
VERSION     KHUT01008678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           96 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtccg
       61 gcagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac ttcggacctc
      121 gagggaggta ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008679
VERSION     KHUT01008679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           88 g           54 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggattta ctaggcgtaa agcgtgggca ggcggttcgg
       61 ttagtctgtt gtgaaagctc ctggcttaac cgggagaggt caatggatac tgccgagctt
      121 gaatgcggga gaggtgactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctat ggcgaaagca ggtcactgga ccgttattga cgctcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01008680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008680
VERSION     KHUT01008680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agagcgcgta ggcggtcctg
       61 taagtcggtg gtgaaatccc ggggcttaac cccggaattg ccctcgatac tgctggactt
      121 gagtgcggga gaggatagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggca gctatctgga ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008681
VERSION     KHUT01008681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggtttgc
       61 taagtcggtt gtgaaagccc atggctcaac catggaagtg cgtccgatac tggcagacta
      121 gagttccgac gaggatggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccggt ggcgaaggcg gccatctggg aggatactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008682
VERSION     KHUT01008682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           95 g           41 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc accgctcaac ggtggaacgg ctgggcatac tggcgacctt
      121 gagacactca ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtgctctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008683
VERSION     KHUT01008683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           90 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggtggttgac
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggttagcta
      121 gagtgtggga tagggaagcg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacgcctgc ggtgtagacg gcttcctgga ccatgactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008684
VERSION     KHUT01008684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           42 c           89 g           61 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggattta ttgggcgtaa agagggcgct ggtggttttg
       61 taagtcggat gtgaaatctc ctggctcaac tgggagtggt cattcgatac tgtgaaactt
      121 gagagcaata gaggtaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg acctactggg ttgcttctga cgcttaggcc cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008685
VERSION     KHUT01008685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           85 g           50 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgg
       61 ttagtacttt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgccgatctt
      121 gagccatcta gaggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008686
VERSION     KHUT01008686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           46 c           82 g           50 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggttgtttat
       61 taagtccgct gttaaatccc acggcttaac catggacagg cggcggaaac tattaaacta
      121 gagtacggta ggggcagagg gaatttccgg tggagcggtg aaatgcgtag atatcggaaa
      181 gaacacccaa ggcgaaggca ctttgctggg ccgaaactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01008687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008687
VERSION     KHUT01008687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           93 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggacctt
       61 taagtggagg gtgaaatctc agggcttaac cctgaaactg ccttccaaac tgggggtctt
      121 gaggtatgga gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gcttcctggt catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008688
VERSION     KHUT01008688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtgcgg
       61 taagtctgtg gtgaaatctc caagctcaac ttggagcctg ccgcggaaac tgccgtgctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008689
VERSION     KHUT01008689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           88 g           53 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcggctatt
       61 taagttttgt gtgaaatccc atggcttaac catggaattg cacgaaatac tgaatggatt
      121 gagtgtagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctataactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008690
VERSION     KHUT01008690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcagac gtgaaatccc tcggctcaac tgaggaactg cgtctgatac tggttggctt
      121 gagtgtggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccacgactga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008691
VERSION     KHUT01008691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggcgggg
       61 aaagttcggg gtgaaatctc caggctcaac ttggaaattg ccttgaagac ttcctcgctg
      121 gagggtcgca gaggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg ggtctctggg cgacacctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008692             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008692
VERSION     KHUT01008692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           65 c           99 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtcggta cctgaaagcc cccggctcaa ccggggaact ggggccgata cgaccggcct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcctgctg acgctgagac gcgaaagcca
      241 gggtagcgaa cggg
//
LOCUS       KHUT01008693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008693
VERSION     KHUT01008693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           89 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttagtt gtgaaagacc tgggcttaac ctagggttgt caactaagac tgttagactt
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008694
VERSION     KHUT01008694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           82 g           54 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggctatg
       61 aaagtcagaa gtgaaatccc agggcttaac cctggaattg cttttgaaac tccatagcta
      121 gaatccggga gaggttagta gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgtaggcg gctaactgga ccggcattga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008695
VERSION     KHUT01008695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           99 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccac
       61 tcagtccggg gtgaaatctt tcggcttaac cggaaacgtg ccttggagac gggtggtctg
      121 gagggagata ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008696
VERSION     KHUT01008696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           77 g           61 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggactgg
       61 taagtcagtg gtgaaatctc cgagcttaac tcggaaattg ccattgatac tgtcggtctt
      121 gagtatagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctgacgaaa ctataactga cactgaggct cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008697
VERSION     KHUT01008697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           87 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtcgga
       61 taagtttgat gtgaaatctt cgggcctaac ccggaaactg cattgaatac tattcggctt
      121 gaggtctgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cagatcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008698
VERSION     KHUT01008698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           88 g           47 t
ORIGIN      
        1 cacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtccgg
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaattg cgctcaaaac tgccggactt
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008699
VERSION     KHUT01008699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          106 g           37 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggcggtgagg
       61 caagtctgct gtgaaagcgc ccggcttaac tgggcgaggt cggcggaaac tgccccactg
      121 gaggccggta gaggcgggta gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaataccagt ggcgaaggcg gcccgctggg ccgagcctga cgctgagacg cgaaggcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008700
VERSION     KHUT01008700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 taagtttcgt gtgaaatccc agtgcttaac gctggaactg cacggaaaac tgttgaattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008701
VERSION     KHUT01008701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggccatc
       61 ttagtcagac gtgaaatccc tcggctcaac cggggaactg cgtctgatac tggatggctc
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgcttaagcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008702
VERSION     KHUT01008702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           98 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcgggttgt
       61 caagtggtgt atgaaatcgc ccggcttaac cgggcgctgt taggccagac tgacagcctg
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccagc ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008703
VERSION     KHUT01008703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttaaa
       61 tgagtgtgat gtgaaagccc agggcttaac cttggaagtg catcgcaaac gatttaactg
      121 gagtatatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008704
VERSION     KHUT01008704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c           97 g           48 t
ORIGIN      
        1 tacgtaggtg tcgagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggcttgg
       61 caagtttggg gtgaaatctc ccggctcaac tgggagggtg cctcgaaaac tgccaggctt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008705
VERSION     KHUT01008705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcgggctcg
       61 caagtgcttt gtgaaatccc acggctcaac cgtggaattg ctcggcagac tgcgagtctt
      121 gaggtcggta gaggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgat ggtgaagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008706
VERSION     KHUT01008706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agagcgcgta ggcggttttt
       61 taagtcggtg gtgaaatccc ggggcttaac ctcggaactg cctccgatac tggaagactt
      121 gagtgcggga gaggatagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggca gctatctgga ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008707
VERSION     KHUT01008707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          106 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggcttca
       61 tgcgtcgtgc gtgaaaggcc ccggctcaac cgggggcggt cgtgcgatac ggtgaagctg
      121 gaggccagta gggggacgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcgtcctgga ctgggcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01008708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008708
VERSION     KHUT01008708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c          100 g           47 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctttg
       61 tttgtcaggc gtgaaagccc cgggcttaac ctgggaactg cgtttgatac ggcagggcta
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008709
VERSION     KHUT01008709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttt
       61 caagtcaggt gtgaaagccc tcggcttaac cgaggaagtg cgcctgaaac tgatgagctt
      121 gagtaccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008710
VERSION     KHUT01008710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           91 g           48 t
ORIGIN      
        1 gacggaggcc ccgagcgttg ttcggaatta ctgggcttaa agcgcgtgta ggcggatcgg
       61 taagtgcctt gtgaaatccc cgggctcaac ccgggaattg ctcggcatac tgccaatctt
      121 gaggcaggta tgagtcgctg gaaccttagg tgtagcggtg aaatgcgtag atatctaagg
      181 gaacgccggt ggcgaaagcg ggcgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01008711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008711
VERSION     KHUT01008711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcacgg
       61 taagttgggt gtgaaatctc cgagcttaac tcggaaacgg cattcaaaac taccgggctg
      121 gagggtcgga agggagactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008712
VERSION     KHUT01008712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           99 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agcgtgcgca ggtggtttcg
       61 taagtcgggc gtgaaagcct tcggctcaac cgagggaggt cgttcgatac tgcgaggctt
      121 gaggtcagga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggc ctgcacctga cgctgaggca cgaaagcgtg
      241 gggagcgatc ggg
//
LOCUS       KHUT01008713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008713
VERSION     KHUT01008713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattta ctgggcgtaa agcgcatgta ggcggctggg
       61 taagttggct gtgaaatctc ccggctcaac tgggagaggc cggtcaagac tgcctggctg
      121 gaggctggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccagacctga cgctcagatg cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01008714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008714
VERSION     KHUT01008714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggcctga
       61 aaagttggat gtgaaatccc acagcttaac tgtggaactg cattcaaaac tttcaggctc
      121 gagtgtgata ggggtaaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg ctttactgga tcactactga cgctgaggga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008715
VERSION     KHUT01008715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           91 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcggggata
       61 caagtcagat gtgaaattcc acggcttaac tgtggcgctg catctgaaac tgtgtctctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008716             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008716
VERSION     KHUT01008716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggtggggtgg
       61 tacgtccgtc gttgaaagcc cccggctcaa ccggggaacg ggcgcggata cggccatcct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttttctg acactgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008717
VERSION     KHUT01008717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           87 g           50 t
ORIGIN      
        1 gacggaggga gcaagcgttg ctcggaataa ttgggcgtaa agggtgcgta ggcggttttt
       61 taagtgagat gttaaagacc cggacttaat ctgggagctg catttcatac tgaaagactc
      121 gagtgtggga gaggaaacag gaattcctca tgtagaggtg aaattcgtag atatgaggag
      181 gaacaccaga ggcgaaggcg agtttctgga acacaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008718
VERSION     KHUT01008718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggccatt
       61 taagtcagac gtgaaatctt cgggcttaac ctgaaaattg cgtttgaaac tggatggctt
      121 gaggaccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cggcacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008719
VERSION     KHUT01008719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggccagg
       61 taagtcagat gtgaaaaccc ttggcttaac caaggaagtg catttgaaac tgtctggctt
      121 gagtacggga ggggaaagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctttctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008720
VERSION     KHUT01008720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           93 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttgcg
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tgcgtgactt
      121 gagtatggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggtgtagacg gctttctgga ccatcactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008721
VERSION     KHUT01008721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacggggggc gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtctga
       61 taagtcaggc gtgaaatccc tcggctcaac cggggaactg cgtttgaaac tgtcggactc
      121 gagtcctgga gagggtggcg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008722             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008722
VERSION     KHUT01008722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           95 g           43 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttccg
       61 tcagtcgggt gtgaaaactc tgggctcaac ccagagacgc cacccgatac tgcggtgact
      121 agagtccggt aggggagtgc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa cggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008723
VERSION     KHUT01008723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcctt
       61 caagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tgagggactt
      121 gaggccatga gaggggtatg gaattcccgg tgtagtggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaagcg gtaccctggc atgggcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008724
VERSION     KHUT01008724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 taagtcagat gtgaaagtcc atggctcaac catggaagtg catttgatac tgaaaggctt
      121 gagtactgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg accatctggc cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008725
VERSION     KHUT01008725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcttgt
       61 taagtcggtt gtgaaaaccc atggctcaac catggaagtg cgtccgaaac tggcaagcta
      121 gagtcttgac gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg aaggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008726
VERSION     KHUT01008726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           48 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agggcgcgta ggcggatcgt
       61 gaagtcggtt gtgaaatccc tcagctcaac tgaggaactg cagccgatac tgccgatctc
      121 gaggacgaaa ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg actctctggt tcgtatctga cgctgaagcg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008727
VERSION     KHUT01008727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcaggg
       61 caagtctgaa gtgaaaggcg ggggctcaac ccccggactg ctttggaaac tgtccggctg
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008728
VERSION     KHUT01008728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtttgg
       61 taagtttggc gtgaaagccc cgggcctaac ccgggaatgg cgctgaaaac tgtcgaactt
      121 gaggattgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacatcagt ggcgaaggcg ggtccctgga caaatcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008729
VERSION     KHUT01008729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           57 c          107 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcttcg
       61 taagtcggcc gtgaaatctc caggctcaac ttggaggggt cgggcgagac tgcggagctc
      121 gaggcaggta gagggcggtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctggg cctgacctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01008730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008730
VERSION     KHUT01008730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac tcggaaactg ccgttgatac tatctgtctt
      121 gaattctgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cagcgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008731
VERSION     KHUT01008731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           88 g           45 t
ORIGIN      
        1 gacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggatttt
       61 taagtgcctt gtgaaatcct gcggctcaac cgcagaactg ctgggcatac tggaagtctt
      121 gggtcgtcca ggggcaaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggttgctgga gacgtaccga cactgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008732
VERSION     KHUT01008732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 tacggggggt gcaaacgttg ttcggaatca ctgggcgtaa agagcgcgta ggcggccggg
       61 taagtggggt gtgaaatcca tcggctcaac cggtgaactg cattccatac tacccggctt
      121 gagggcagca gaggagacgg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cgtctctggg ctgctcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01008733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008733
VERSION     KHUT01008733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggccgag
       61 caagtcgggc atgaaatctc tcggctcaac cgggaggggt tgtccgatac tgctgggctt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgtacctga cgctcaaacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008734
VERSION     KHUT01008734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           84 g           51 t
ORIGIN      
        1 tacggagggt ccaagcgtta ttcggaatta ctgggcgtaa agcgcacgta ggtggtttac
       61 taagtcagat gtgaaagccc tggactcaat ccaggaagtg catttgatac tggtagacta
      121 gagtgcagga gaggaaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga agcgaaggcg gctttctgga ctgcaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008735
VERSION     KHUT01008735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c          100 g           39 t
ORIGIN      
        1 gacagaggtg gcaagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggtggctaga
       61 taagtccggt gtgaaatccc acggcttaac cggggaatgg caccggagac tgtatagctc
      121 gaggacggga gaggagagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gctctctaga acgtccctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008736
VERSION     KHUT01008736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          100 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta cccggattta ctgggcgtaa agggtgcgta ggcggctggg
       61 taagtctcct gtgaaagctc ccggcttaac tgggagaggt caggggatac tactcggctt
      121 gagggcagta gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg ctgcatctga cgctgaggca cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01008737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008737
VERSION     KHUT01008737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtcggat gtgaaatctc caagctcaac ttggaaatgg cattggaaac tgccctgcta
      121 gaggatcgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cgatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008738
VERSION     KHUT01008738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggctttc
       61 taagttggat gtgaaaactc tgggctcaac ccagagactg catccaaaac tgggaggcta
      121 gggtcctgga ggggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga caggtaccga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008739
VERSION     KHUT01008739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggtctgg
       61 aaagttgggt gtgaaatccc tcggcctaac cgaggaattg cgctcaaaac tcccagactt
      121 gaggaggata gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gcttactggg tcctttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008740
VERSION     KHUT01008740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           88 g           53 t
ORIGIN      
        1 tacagaagcc tcgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggtgacg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaagtg catttgatac tgcgttgcta
      121 gagtgcgaga gaggagagcg gaattcccgg tgtaggggtg aaatccgtag atatcgggag
      181 gaataccgat ggcgaaggca gctctctggc tcgtaactga cactgagatt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008741
VERSION     KHUT01008741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           84 g           59 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttta
       61 taagtcagga gtgaaatccc ggagcttaac ttcggaattg cttttgaaac tataaggcta
      121 gagtaagtta gaggatgtcg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gacatctggg acttaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008742
VERSION     KHUT01008742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           95 g           43 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcagg
       61 taagtcaact gtgaaagccc agggctcaac cttggaagtg cagctgaaac tacctgacta
      121 gagtctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accgcctgga cgaagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008743
VERSION     KHUT01008743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggtcat
       61 taagtggtgt atgaaatcgt ccggctcaac cgggcgaggt tatgccagac tgatggtcta
      121 gagagcgaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcatctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008744
VERSION     KHUT01008744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          100 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcgggagga
       61 gaagtccggt gggaaagccc acggctcaac cgtggaagaa cgctggaaac tctctttctt
      121 gagggcggca gagggaagtg gaattgctgg tgtagcggtg aaatgcgtag atatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctggg ccgcccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008745
VERSION     KHUT01008745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggacttg
       61 caagtcagat gtgaaatctc ggggctcaac ctcgaaactg cgtctgaaac tgcaggccta
      121 gaatgtcgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggt ggcgaaggcg cctacctgga cgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008746
VERSION     KHUT01008746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           97 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcaagca ggcggcggtt
       61 taagtctgac gtgaaatctc ctggcttaac tgggagggga cgttggaaac tggattgctt
      121 gaggtggtga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg acaccctggc acttgcctga cgctgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008747
VERSION     KHUT01008747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctggg
       61 tacgtgaggc gtgaaagtcc tgggctcaac ccggggacgg cgcttcagac tgcctggctt
      121 gagtccggaa gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggt ccggtactga cgctcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008748
VERSION     KHUT01008748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           96 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcagg
       61 caagtcaggg gtgaaagccc acggctcaac cgtggaagtg cctttgatac tgcctgtctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008749
VERSION     KHUT01008749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           90 g           48 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcttta
       61 taagtgaggt gttaaatccc ccggctcaac cggggacctg cacttcatac tgtttagctt
      121 gagtacagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg ctgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008750
VERSION     KHUT01008750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggatgtt
       61 taagtcagtg gtgaaagccc agggctcaac cttggaactg ccattgatac tggacatctt
      121 gagtgcggga gaggtgagtg gaacaccgag tgtagaggtg aaattcgtag atattcggta
      181 gaacaccagt ggcgaaggcg actcactggc ccgtaactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008751
VERSION     KHUT01008751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           78 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggacata
       61 taagtcagtg gtgaaagcct acagcttaac tgtagaactg ccattgatac tgtatgtctt
      121 gaattcggtc gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat agcgaaggca gctcactagg cctggattga cgctcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008752
VERSION     KHUT01008752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           91 a           28 c           66 g           68 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agggcacgta ggtggtttgt
       61 ttctgtgtat tattaaatta taaagcataa ctttagaatg taatacaaac aacaaaacta
      121 gagttaggta gagggtagta gaatttctaa tgtaaaggtg aaatttgata aaattagaga
      181 gaataccaat ggcgtaagca actacttggg ccttaactga cactgaggtg cgaaggtatg
      241 gggagcaaat agg
//
LOCUS       KHUT01008753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008753
VERSION     KHUT01008753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcgggctgt
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgacagcctt
      121 gagtacagaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008754
VERSION     KHUT01008754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggtcatt
       61 taagtttgat gtgaaatccc tgggcttaac ctaggaactg cattgaaaac tggatgactt
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008755
VERSION     KHUT01008755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           85 g           58 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgat
       61 taagttagat gtgaaatccc tgggcttaac ctaggaattg catttaatac tgattggcta
      121 gagtatggga gaggtaagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg acttactgga ccattactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008756
VERSION     KHUT01008756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           94 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttggg
       61 aaagttggat gtgaaagccc tgggcttaac ctgggaattg catccaaaac tacccggcta
      121 gagtgcagca gaggatggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg actgtctggg ctgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008757
VERSION     KHUT01008757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatgat
       61 caagtcggtg gtgaaatcct cccgcttaac gggagaacgg ccttcgatac tgattgtctc
      121 gagcaaggta ggggtgcgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acgcactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008758
VERSION     KHUT01008758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           98 g           41 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggtgggtgac
       61 caagtcggaa gtgaaatccc agggctcaac cctggggctg ctcccgaaac gggtcgccta
      121 gggactggta gaggctggta gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaatacctgt ggcgaaggcg gccagctggg ccagatccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008759
VERSION     KHUT01008759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggcggcctag
       61 caagtcctgt gtgaaaggtc tcggcttaac cgaggaacgt catgggaaac tgttaggctc
      121 gagtgccgga gagggcagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg actgcctgga cggtaactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01008760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008760
VERSION     KHUT01008760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta gccgggaggg
       61 caagtcagat gtgaaatcca cgggcttaac tcgtgaactg catttgaaac tgttcttctt
      121 gagtatcgga gaggcaatcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gattgctgga cgacaactga cggtgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008761
VERSION     KHUT01008761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           85 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtttgg
       61 aaagtcgggt gtgaaatccc acagctcaac tgtggaactg cactcgaaac ttccagacta
      121 gagtatgaga ggggttaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg cttaactggc tcattactga cgctcaggga cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008762             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008762
VERSION     KHUT01008762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           46 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggattta ttgggcgtaa agggctagta ggcggcttgg
       61 caagtcggat gtgaaaactc caggctcaac ctggagacgc cactcgatac tgccatggct
      121 agagtccggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccggaactg acgctgaata gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008763
VERSION     KHUT01008763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           85 g           53 t
ORIGIN      
        1 tacgagtggt gcgaacgtta ttcggattta ctgggcgtaa agggtgcgta gacggtagag
       61 taagtcaact gttaaatttt ccggcctaac cggaaacagg cggtagaaac tgctttgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008764             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008764
VERSION     KHUT01008764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          100 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggcggttgtt
       61 taagtctatc tgtgaaaggc cggggctcaa ccccgtgctt gcaggagata ctggacgact
      121 ggagtcaggt agaggcgagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccag aggcgaaggc ggctcgctgg acctgtactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008765             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008765
VERSION     KHUT01008765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctccg
       61 tcagtcgggt gtgaaacctc caggctcaac ctggagctgc cacccgatac tgcggtggct
      121 tgagtccggt agggggccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggcctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008766             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008766
VERSION     KHUT01008766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           95 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggtgggttgc
       61 cacgtcggat gctgaaatcc ctcggctcaa ccgaggaacg ggcaccgata cgagcgacct
      121 ggaggggggt agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagctcctgg accttttctg acactgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008767
VERSION     KHUT01008767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttcg
       61 caagtgatgg gtgaaatccc tcggcttaac cgaggaactg cccctcaaac tgcgttgctt
      121 gagtccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008768
VERSION     KHUT01008768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtctgt
       61 cgcgtcggat gtgaaagctc agggctcaac cctgagtctg catccgatac gggcagacta
      121 gagtcaggta ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cctgtactga cgctgaggaa cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008769
VERSION     KHUT01008769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           44 t
ORIGIN      
        1 gacggagggt gcgaacgttg ttcggaatta ctgggcataa agagcacgta ggcggttcgc
       61 taagttgggt gtgaaagccc ccggctcaac cggggaattg ctcccgatac tggcggacta
      121 gagatcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgacactga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008770
VERSION     KHUT01008770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           58 c           91 g           41 t
ORIGIN      
        1 tacgaaccgg acaaacgtta ctcggaatta ctgggcttaa agggtgcgta ggcggcgcga
       61 agggtgggat gtgaaatccc ccggctcaac cggggaattg cgtcccaaac cgccgtgctc
      121 gaggaagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaggcg gctcactggg tcttccctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008771
VERSION     KHUT01008771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          109 g           34 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggctagg
       61 tatgtgcttg gtgacatccc acggctcaac cgtgggcggc ccaggtagac agcctggctg
      121 gaggcaggga gagggtcgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcgacctggc cctgtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008772
VERSION     KHUT01008772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggaatca ctgggcttaa agcgtacgta ggcggagatg
       61 caggcgtcgt gtgaaatccc tcggctcaac cggggaactg cacggcgaac cgcatctctt
      121 gagacaagta ggggcggccg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaatgccgaa ggtgaaaaca ggccgctggg cttgctctga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008773
VERSION     KHUT01008773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggttga
       61 taagtctgtg gtgaaatccc caagcttaac ttgggaactg ccatggatac tattgatctt
      121 gaatatcgcg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacatg
      181 gaacaccaat tgcgtaggca gcttactaca cgatcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008774
VERSION     KHUT01008774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgca ggcgggcttg
       61 taagtcagag gtgaaagcca ccggcctaac cggtgaactg cctttgatac tgcaagtctt
      121 gagtcccgga gaggctatcg gaattcgtgg tgtagcggtg aaatgcgtaa atatcacgag
      181 gaacaccgga tgcgtaagcg gatagctgga cgggtactga cgctcaggca cgaaagcgcg
      241 gggagcgaac agg
//
LOCUS       KHUT01008775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008775
VERSION     KHUT01008775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          101 g           39 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcaggg gtgaaagccc ccagctcaac tggggaagtg cctctgatac tggccgtctc
      121 gagggggata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaagcg gcgcgctggg tcccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008776
VERSION     KHUT01008776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttga
       61 taagtctggg gtgaaagccc gttgctcaac aacggaactg ccctggatac tgtcagactt
      121 gaggacagac gaggttggcg gaatagaaga tgtagcggtg aaatgcatag atatcttcta
      181 gaaccccgat tgcgaaggca gctgactaga ctgtatctga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008777
VERSION     KHUT01008777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          103 g           40 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgta ggtggcttgc
       61 tgcgtcggtg ctgaaagcgc ccggcttaac cgggcgaggc gtgccgatac gagcgagctc
      121 gagcttcgga gagggcggtg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg accgcctggc cgagtgctga cactgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01008778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008778
VERSION     KHUT01008778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           75 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcagc
       61 taagtcagtg gtgaaatcct gcagctcaac tgcaggactg ccattgatac tggttgcctt
      121 gaattaatgt gaagtggtta gaatatgcag tgtagcggtg aaatgcttag atattgcata
      181 gaataccaat tgcgaaggca ggccactaac attacattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008779
VERSION     KHUT01008779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggatgg
       61 taagtcgggc gtgaaatccc caagctcaac ttgggaactg cactcgaaac taccttcctt
      121 gagtgtcgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008780
VERSION     KHUT01008780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           86 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggttttg
       61 aaagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac ttcagaacta
      121 gaggtcagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggtgaagacg gttttctggg ctgatactga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008781
VERSION     KHUT01008781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatca ctgggcgtaa agggtctgta ggcggttcgg
       61 taagtctcct gtgaaaactt cgggctcaac ccggagatgt cgggggatac tgccgggcta
      121 gagaatgtca gaggaaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctttctggg gcatttctga cgctgaggga cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008782
VERSION     KHUT01008782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggtttt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaacag catacgaaac tgtagatctt
      121 gaatgctcga gaggccagtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacaccaga ggcgaaggcg actggctggc gatgcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008783
VERSION     KHUT01008783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          101 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttct
       61 caagtcgggt gtgaaagccc ggggctcaac cccggaacgg cacccgagac tgggtggctt
      121 gagaccggga gaggtgggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctcactggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008784
VERSION     KHUT01008784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttaat
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaatgg catatgaaac tggttggcta
      121 gagttcggga gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc ccgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008785
VERSION     KHUT01008785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccagg
       61 taggtccgac gtgaaatctc gaggctcaac ctcgagcggt cgttggatac cgcctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaagca gctctctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008786
VERSION     KHUT01008786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           42 c           74 g           58 t
ORIGIN      
        1 tacaggggat gcgagtgtta tccggaatta ttgggcgtaa agcgtctgta ggttgatagt
       61 taagtctttt gtcaaagctc cgggcttaac ccggaatacg caaaggaaac tgcctatctt
      121 gagtatggta gaagtaaagg gaattttcag tggagcggtg aaatgcgtag agattggaaa
      181 gaacaccaaa ggcgaaagca ctttactaga ccattactga cactcagaga cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008787
VERSION     KHUT01008787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agagcgcgta ggcgggaatt
       61 taagtcagga gtgaaaacta ccggctcaac tggtagcctg ctcttgaaac tggatttctt
      121 gagggtatga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctggc atacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008788
VERSION     KHUT01008788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           97 g           44 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggtggcccga
       61 caagtcggct gtgaaagctc ctggcttaac tgggagaggc cagtcgaaac tgtggggctt
      121 gagattggga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc ccacatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008789
VERSION     KHUT01008789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           91 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgttc aacggattca ttgggcttaa agcgtacgta ggcgggtgtg
       61 caggcgtcgt gtgaaagccc tcggctcaac cgaggaattg cacggcgaac cgcacatctt
      121 gagacaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgat ggtgaagaca ggccgctggg cttgttctga cgctgaggta cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008790
VERSION     KHUT01008790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           99 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtgcgt
       61 taagtctgat gtgaaagccc tgggctcaac tcaggaactg cattggagac tggcgtgcta
      121 gagtaccgga gagggaagcg gaattcctaa tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gcttcctggc cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008791
VERSION     KHUT01008791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           84 g           55 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agcgtatgta ggcggcttcg
       61 taagacagat gtgaaatccc agagcttaac tttggaactg catttgtgac tgcaaggctt
      121 gagtatcgga gaggttggcg gaattcctag tgtagaggtg aaattcgcag atattaggag
      181 gaacaccgat ggcgtaagca gccaactgga cgattactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008792
VERSION     KHUT01008792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttatc
       61 taagtctggc gtgaaatctc ccggctcaac tgggaggctg cgttagatac tggatagctt
      121 gagcgtggga gaggaaagcg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacacctgc ggtgaagaca gctttctgga ccacagctga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008793
VERSION     KHUT01008793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 gacgtaggaa gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggggcgg
       61 cgcatccggg agtaaatccc acggcttaac cgtggaacca tctcggagat ggccgctctt
      121 gaggacagga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008794
VERSION     KHUT01008794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggtcgcg
       61 caagtccgtt gtgaaatcct agggctcaac cctagaactg caatagaaac tgcacggctt
      121 gagtactgga gagatgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaataccagt ggcgaaggcg cctcattggc cagtaactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008795
VERSION     KHUT01008795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           98 g           42 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtggac
       61 taagtctggc gtgaaagctc ccggctaaac cgggagaggt cgttggatac tggttgacta
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cgctaagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008796
VERSION     KHUT01008796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtccgat gtgaaatctc cgagcttaac tcggaaacgg catcggatac tattcggctc
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgattcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008797
VERSION     KHUT01008797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttgca
       61 caagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgaaac taggcgacta
      121 gagtctcgga gaggaaagcg gaattctggg tgtagaggtg aaattcgtag atatccagag
      181 gaacaccggt ggcgaaggcg gctttctgga cgagtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008798
VERSION     KHUT01008798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggattta ctgggtgtaa agggcacgta ggcggcccga
       61 taagctctgc ctaaaatgca ctggcttaac cagggcgtgt ggtggaggac tgctgggctt
      121 gaggatcaga gaggcgagcg gaattcccgg tgtaagggtg aaatctgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctcgctggc tgatttctga cgctaaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008799
VERSION     KHUT01008799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           88 g           47 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ctcggaatta ctgggcataa agcgcacgta ggttgcgaga
       61 taagcgagga atgaaatccc atggctcacc catggaactg ttcctcgaac tgtcttgctt
      121 gagggcggga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg actctctggc ccgtccctga cactgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008800             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008800
VERSION     KHUT01008800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           51 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccagagaggc cactcgatac tgctatgact
      121 tgagtacggt aggggagtgg ggaatttcta gtgtagcggt gaaatgcgca gatattagaa
      181 ggaacaccag tggcgaaggc gccactctgg gccgtaactg acgctgagga gcgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008801             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008801
VERSION     KHUT01008801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           52 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctggcaggct
      121 agaggaatgc agaggtaagt agaattcctg gtgtagcggt gacatgcata gatatcagga
      181 ggaataccca tggcgaaggc agcttactgg gcattatctg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008802             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008802
VERSION     KHUT01008802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcaggt gttaaagctc cgagctcaac tcggagaggc catttgatac tgctgtgact
      121 agaatccagt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gctggaattg acgctgagga gcgaaagtgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008803
VERSION     KHUT01008803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtccggg gtgaaatcct ccagcttaac tggagaacag ccttggatac tggtggcctg
      121 gaggaaggta ggggcatgtg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008804
VERSION     KHUT01008804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           51 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggattta ctgggcgtaa agagtctgta ggcggcgggg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catccgatac tgccttgctt
      121 gagtattgga ggggagcctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacactagt ggcgaaggcg aggctctgga caattactga cgctgagaga cgaaggtcag
      241 gggagcgaaa agg
//
LOCUS       KHUT01008805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008805
VERSION     KHUT01008805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattca ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtccgac gtgaaaactg gaggctcaac ctccagctgt cgtcggaaac cctctggcta
      121 gagtccggaa gaggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008806
VERSION     KHUT01008806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggaccat
       61 caagtcgggg gtgaaatccc ggggctcaac cccggaactg cctccgaaac tgttggtctg
      121 gaggatgaga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc tcatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008807
VERSION     KHUT01008807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           94 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggataat
       61 caagctggat gtgaaagacc tgggcttaac ctgggggtgt cattcgggac tggttgtcta
      121 gagtagagga gaggggagtg gaattcacag tgtagcggtg aaatgcgtag atattgtgaa
      181 gaacatcgat ggcgaaggca gctctctggc ctcatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008808
VERSION     KHUT01008808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggcggccaac
       61 taagtcagac gtgaaatccc ccggctcaac cggggaactg cgtctgatac tggatggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgagaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008809
VERSION     KHUT01008809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           89 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatga ttgggcgtaa agggtgcgta ggtggcagat
       61 caagtctgga gtaaaaggta tgggctcaac ccgtactggc tctggaaact gatcagctag
      121 agaacagaag aggacggcgg aactccatgt gtagcggtaa aatgcgtaga tatatggaag
      181 aacaccggtg gcgaaggcgg ccgtctggtc tggattctga cactgaagca cgaaagcgtg
      241 gggagcaaat agg
//
LOCUS       KHUT01008810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008810
VERSION     KHUT01008810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           48 c           84 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaataa ctgggcgtaa agggcatgca ggcggttcat
       61 caagtaggat gtgaaatccc cgggctcaac ctgggaacag catactaaac tggtggacta
      121 gagtattgca gggggagacg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccaaa ggcgaaggca gtctcctggg caaatactga cgctcatatg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008811
VERSION     KHUT01008811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttcg
       61 caagttgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccaaaac tacggagctt
      121 gagtaccgga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgagtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008812
VERSION     KHUT01008812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcaggg
       61 tgtgtcggat gtgaaatccc atggctcaac catggaagtg cacccgaaac tacccagctc
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008813
VERSION     KHUT01008813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           93 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggctgcctgc
       61 ttagtcgggg gtgaaatctt tcggcttaac cggaaaattg ccttcgatac tggcaggctc
      121 gagcgaggta ggggcggatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008814
VERSION     KHUT01008814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           56 c           75 g           48 t
ORIGIN      
        1 tacagagacc ccgagcgtta tccggaatta ctgggcgtaa agcgcgagca gccggtcgca
       61 ttagtctgat gtaaaaccat ggggcccaac tccatgtccg cattggaaac gttgcgactc
      121 gagtagataa gaggagagcg gaacgtatgg tgtaggggtg aaatccactg atatcatacg
      181 gaacaccaaa agcgaaggca gctctctagt gtcttactga cggtcaatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01008815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008815
VERSION     KHUT01008815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          101 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggcggcctgt
       61 taagtcccgt gtgaaaggtt tcggctcaac cggaacaggc cacgggaaac tggcgggctt
      121 gagggccgga gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccatctgga cggtccctga cgctgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01008816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008816
VERSION     KHUT01008816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           97 g           52 t
ORIGIN      
        1 tacgggggga gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggttgag
       61 taagtcaggg gtgaaatgcc agggcttaac cttggaattg cctttgatac tgcttggctg
      121 gaggacggaa gaggatagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctatctggt ccgtttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008817             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008817
VERSION     KHUT01008817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           85 g           55 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttagcg gtgggaaatc ggggctcaac cccgtggaag tcggtaatac tgttagacta
      121 gagggctgga gaggttaacg gaatggttca tgtaggggta aaatccataa atatgaattg
      181 gaacaccaaa cggcgaaggc agttaactat tcagttcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01008818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008818
VERSION     KHUT01008818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           90 g           43 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcgcgta ggcggtcctg
       61 tatgtcgatt gtgaaagccc cgggctcaac ctgggaacag cagtcgaaac tgcaggactt
      121 gaatgttgga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgcctggc caaacattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008819             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008819
VERSION     KHUT01008819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           94 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggcttct
       61 taagtttggg gtgaaaggtt caacgctcaa cgtgaacagt gccttgaata ctgggaggct
      121 tgaatgtgac agagggtcgt ggaatttccg gtgtagcggt gaaatgcgta gatatcggaa
      181 ggaacaccaa tggcgaaggc agcgacctgg gttaacattg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01008820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008820
VERSION     KHUT01008820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           84 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttga
       61 aaagtcagat gtgaaatccc cgggctcaac ctgggaactg cgtttgaaac tctcaaacta
      121 gagtatggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccaat ggcgaaggca gccccctggg tcaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008821             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008821
VERSION     KHUT01008821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           64 c           98 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatcgg
       61 cacgtcggac gctgaaatcc cccggctcaa ccggggaacg ggcgccgata cgaccgatct
      121 ggagggaggt agggggagcc ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gggctcctgg accttgcctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008822
VERSION     KHUT01008822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          100 g           47 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgtcc gtgaaaacct gtggcttaac tacaggcttg cggtcgatac gggcaggcta
      121 gagtgcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgctactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008823
VERSION     KHUT01008823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           88 g           53 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggctata
       61 caagtctggt gtgaaatccc agggctcaac cctggaactg cattggaaac tgtatggctt
      121 gagtaccgga gaggtgaagg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cttcactggc cggttactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008824             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008824
VERSION     KHUT01008824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           80 g           54 t
ORIGIN      
        1 cacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggttgaa
       61 taagttactg gtggcaaatc ggggcccaac cccgtggaag tcggtaatac tattcaactc
      121 gaggaccgga gaggttaacg gaattctatg tgtagcagta aaatgctcaa atatatagag
      181 gaacaccaaa cggcgaaggc agttaactat tcggttcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01008825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008825
VERSION     KHUT01008825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           66 c          104 g           33 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 cgcgtccggg gtgaaatcct ccggctcaac cggagaacgg ccccgggtac gggcggcctc
      121 gaggggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga cccccactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008826
VERSION     KHUT01008826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 tacgaggggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcctgg
       61 tcagtcggat gtgaaatccc tgggctcaac ctgggaactg cattcgatac tgccaggcta
      121 gagtgtggga gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accacctggc ccaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008827
VERSION     KHUT01008827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           76 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtgggccgt
       61 taagtcagag gtgaaatcct gcagctcaac tgtagaattg cctttgatac tggcggtctt
      121 gaatcattgt gaagtggtta gaatatgtgg tgtagcggtg aaatgcatag atatcacata
      181 gaataccgat tgcgaaggca gatcactaac aatgcattga cactgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008828
VERSION     KHUT01008828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          109 g           34 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggtgcgg
       61 taggtagctg gtgacagtcc gccactcaat ggcgggaggc ctggctagac cgccggactg
      121 gaggcaggga gagggtgatg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagt ggggaagccg gtcacctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagccaac ggg
//
LOCUS       KHUT01008829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008829
VERSION     KHUT01008829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggagagt
       61 taagtgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcaaac tggctttctt
      121 gagggcgaca gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcactctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008830
VERSION     KHUT01008830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagcacgta ggtggttgtt
       61 taagttggat gtgaaagccc cgggcttaac ctgggaaagt cattcaaaac tgggcgacta
      121 gagtacggta gagggaagtg gaatttccgg tgtagcggtg aaatgcatag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008831
VERSION     KHUT01008831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           86 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggtctt
       61 taagtcagcg gtgaaattct ccggctcaac cggggacgtg ccattgatac tggggacctt
      121 gagtacagac gaggtgggtg gaatttacgg tgtagcggtg aaatgcatag ataccgtaaa
      181 gaaccccgat agcgaaggca gcccactagg ctgatactga cgctgatgca cgaaagcatg
      241 gggatcgaac agg
//
LOCUS       KHUT01008832             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008832
VERSION     KHUT01008832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcacgt gtgaaatctc cgcgctcaac gcggaggcgc cacgtgatac tgctgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc gacactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008833
VERSION     KHUT01008833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacagagcct gcgagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgtgaggc gtgaaagccc gatgcttaac atcggaattg cacctcaaac tacatggcta
      121 gagcattgga gagggtagca gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaataccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01008834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008834
VERSION     KHUT01008834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggcggtttgg
       61 taagtctgtt gtgaaagcct cgggctcaac ccggggaggc caatggatac tgccggactt
      121 gaatgtggga gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaagca ggtctctgga ccactattga cgctcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01008835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008835
VERSION     KHUT01008835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          108 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcgggaggc
       61 caagtcggtc gtgaaatcct cgggctcaac ccgagggggt cggccgaaac tggttttctt
      121 gaggggcgca gaggagcctg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gggctctggg cgcaccctga cgctgaggcg cgaaggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008836
VERSION     KHUT01008836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           89 g           46 t
ORIGIN      
        1 aacgtaggaa gcaagcgtta tccggattta ctgggcgtaa agggcaggca ggcggttggt
       61 taagttggac ttgaaagctc tcggctcaac tgggagaggc ggttcaaaac tagccaacta
      121 gaggatgata gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg tcacacctga cgctcaactg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01008837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008837
VERSION     KHUT01008837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agagcgtgta ggcggtttgg
       61 caagtcagat gtgaaagccc tgagctcaac tcaggaagtg catttgaaac tgctttactt
      121 gagtatggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacacccgt ggcgaaggcg actctctgga ccaatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008838
VERSION     KHUT01008838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatca ctgggcttaa agggcgcgta ggtggtagtg
       61 taagtctgag gtggaatcct gcggcttaac cgcagaactg ccttggaaac tgcattactt
      121 gagtcagtga ggggagggta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaaggcctgt ggcgaaggcg gccctctggc gctggactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008839             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008839
VERSION     KHUT01008839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           86 g           47 t
ORIGIN      
        1 tacggagaac tcaagcgtta cccggaatca ctgggcgtaa agcgttgcgt aggcggcttt
       61 gcaagtcggg tcttaaaggc cggggctcaa cctcggcagt gggcttgata ctacaaagct
      121 agaggatggt agaggtgagt ggaattgctg gtgtaggagt gaaatccgta gatatcagca
      181 ggaacaccaa tggcgaaggc agctcactgg accattcctg acgctcaggc acgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01008840             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008840
VERSION     KHUT01008840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           94 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcaga
       61 ctaagtccaa tgtgaaatac caaggcttaa ccttggcagg tcgttggata ctggtcgact
      121 agagtgtgga aggggttgct ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacaccag aggcgaaggc gggcaactgg gccaacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008841             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008841
VERSION     KHUT01008841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c          106 g           33 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccgc
       61 acaagtccgc ggtgaaaacc tccggcttaa ccgggggccg gccgaggaaa ctgtcgggct
      121 ggaggctggg agagggaagc ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 ggaacaccgg tggcgaaggc ggcttcctgg accagccctg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008842
VERSION     KHUT01008842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           86 g           49 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 caagtcggga gtgaaatccc tcagctcaac tgaggaactg ctttcgatac tgccgtgctt
      121 gagtgagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gcttactggg tctcaactga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01008843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008843
VERSION     KHUT01008843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          103 g           43 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggcctgt
       61 taagttcggc gtgaaagctc ccggctcaac tgggagaggg cgttggatac tggcaggctt
      121 gagtcttgga gaggtgcgtg gaattcccgg tggagtggtg gaatgcgttg agatcgggag
      181 gaacaccaga ggcgaaggcg acgcactggc caacgactga cgctgaagcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008844
VERSION     KHUT01008844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           85 g           57 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtccgca ggcgggcaat
       61 taagtcaggg gtgaaatggt gcggctcaac cgtagcactg cctttgatac tggttgtctt
      121 gagttatggt gaagtggccg gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaacaccgat tgcgaaggca ggtcactaac catggactga cgctgatgga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008845
VERSION     KHUT01008845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           83 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcagtg ctgaaatacc ccggctcaac cgggggggtg gcattgatac tgcagggctt
      121 gagtaaagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaactccaat agcgaaggca gcttactaaa cttcaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008846
VERSION     KHUT01008846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           96 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattca ctgggcgtaa agcgcgcgca ggcggtcttc
       61 tacgtctggg gtgaaaaccc ggggctcaac cccgggactg ccttggatac gggaagactt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008847
VERSION     KHUT01008847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           38 c           80 g           59 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtttga
       61 taagtctgat attaaataca gaagcttaac ttctgaactg tatcggaaac tgtcagacta
      121 gaggacaata gaggaagtgg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg cacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008848
VERSION     KHUT01008848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          101 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcttgt
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaggtg cgcttgggac tggcgagcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008849
VERSION     KHUT01008849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           99 g           45 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggatggc
       61 caagttggtt gtgaaagcgc ccggctaaac tgggcgaggc cgatcaagac tggttgtctc
      121 gagagtgata gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggggaagccg gcgccctggg tcatatctga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008850
VERSION     KHUT01008850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctgg
       61 taagtcggac gtgaaagccc ggggctcaac cccggacgtg catccgaaac tgcctggctt
      121 gaatgccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cggtcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008851
VERSION     KHUT01008851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttatc
       61 taagtttgat gtgaaatccc tgggcttaac ctaggaattg cattgaatac tggataactg
      121 gagtactgca gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008852
VERSION     KHUT01008852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c          101 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggctccg
       61 cgagtcgggg gtgaaatcgt tccgctcaac gggacaaggg cctccgagac cgcgggactc
      121 gagggggata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tccctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008853
VERSION     KHUT01008853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           89 g           47 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatcgta
       61 caagtcttat gtgaaagccc agagcttaac tctggaagtg cacgagatac tgtacgaatt
      121 gagttccgga gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg atcttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008854
VERSION     KHUT01008854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           98 g           43 t
ORIGIN      
        1 aacgtaggag acaagcgtta tccggagtta ctgggcgtaa agcgcacgca ggcggtgatg
       61 taagtgtggc gtgaaagccc tcggctaaac tgggggagga cgaaacagac tgcatgacta
      121 gagtgggata gagggacgtg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgaaaacg gcgtcctggg tcctgactga cgctgaggtg cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008855
VERSION     KHUT01008855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcaggttcg
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaaggg ccttggatac tgcgagtctc
      121 gagggaggta ggggcctgtg gaactttggg tggagcggtg aaatgcgttg atatccaaag
      181 gaacgccggt ggcgaaagcg acgggctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008856
VERSION     KHUT01008856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtttgg
       61 taagtcggaa gtgaaagccc tcggcttaac cgaggaagtg ctttcgaaac taccaggctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008857
VERSION     KHUT01008857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggctttt
       61 caagttggct gtgaaagctc ccggcttaac tgggagaggt cagtcaaaac tggaaggctc
      121 gaggctggaa gaggcgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctcgctggt ccagccctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008858             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008858
VERSION     KHUT01008858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           78 g           61 t
ORIGIN      
        1 tacagagggt gcaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggcggtttc
       61 aaaagttatc tttcaaatac cacggctcaa ccgggggaag ggagataata ctgtgagact
      121 tgattattgg tggggtttct ggaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaactccta gggcgaaggc aagaaactaa ccaattaatg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01008859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008859
VERSION     KHUT01008859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           83 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggatggt
       61 taagtaagaa gtgaaaggcg ggagctcaac tcccggactg cttcttatac tggccgtctt
      121 gaatgtcgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca gctttctgga cgaatattga cgctcatgca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01008860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008860
VERSION     KHUT01008860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           90 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggagcgta ggcggttaat
       61 taagttagat gtgaaatacc cgggcttaac ttggggggtg catctaatac tggtaaacta
      121 gagtacagga gaggaaagcg gaattcctag tgtagcggtg aaatgcatag atattaggag
      181 gaacatcggt ggcgaaggcg gctttctgga ctgacactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008861
VERSION     KHUT01008861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 tacgtagggt gctaacgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggttaca
       61 caagttggat gtgaaatccc tgggcttaac cgaggaagtg catccaaaac taggtagcta
      121 gaggacggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaatacctgt ggcgaaggcg gccttctggg ccgttcctga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008862
VERSION     KHUT01008862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggttggg
       61 taagtctgat gtgaaatccc ggagcttaac tccggaactg catcggatac tatccagctc
      121 gaggattgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctgga caattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008863
VERSION     KHUT01008863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 gacagaggcc ccgagcgtta atcggaatta ctgggcttaa agggtgcgta ggcggaccag
       61 aaagtgcttt gtgaaagccc ccggctcaac cggggaacag cggggcatac tgctggtctt
      121 gaggaaggta ggggccgcga gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaatgccgat ggtgaagacg cgcggctggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008864
VERSION     KHUT01008864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacgtaggga gcgagcgtta tccggattta ctgggtgtaa agggtgcgta ggcggcctgg
       61 caagtcagaa gtgaaattta ggggctcaac ctctaaactg cttctgaaac taccgggcta
      121 gagtgcagga gaggaaagcg gaactccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt agcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008865
VERSION     KHUT01008865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           99 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggccgat
       61 caagtcgggt gtgaaagccc ggggctcaac cccggaacgg cacccgatac tggttggctc
      121 gagaccggga gaggagggtg gaatatccag tgtagaggtg aaattcgtag atattggatg
      181 gaacaccagt ggcgaaagcg gctctctggc ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008866
VERSION     KHUT01008866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 gacgaactgc ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcaacc
       61 caagtcaggg gtgaaatccg gcagcttaac tgtcgaagtg cctttgatac tgagttgctc
      121 gaggaaggta ggggtctgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acggactgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008867
VERSION     KHUT01008867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttttt
       61 taagtcgagt gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac taactgacta
      121 gaagatggca gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctgga ccattcttga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008868
VERSION     KHUT01008868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           96 g           47 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg ccttggatac tgctggtctc
      121 gagggaggta ggggcgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008869
VERSION     KHUT01008869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcatgta ggcggcttgt
       61 taagttggtc gtgaaagccc atggctcaac catggaagtg caaccaaaac tggcaagctt
      121 gagttctgaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gccatctggg aagcgactga cgctgagatg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008870
VERSION     KHUT01008870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           94 g           43 t
ORIGIN      
        1 aacgtaggaa gcgagcgtta tccggattta ctgggcgtaa agggcaggca ggcggttggc
       61 taagtcctac ttgaaagctc ccggctcaac tgggagaggc ggtgggaaac tagcggacta
      121 gaggatggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccatacctga cgctcaactg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01008871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008871
VERSION     KHUT01008871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           51 c           76 g           54 t
ORIGIN      
        1 tacgtaggtc gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta tgtggttttg
       61 ccagtccgtg gttaaatccc agagctcaac tctggaatcg tcatggaaac tacaaaactt
      121 gagtagtgca ggggcagatg gaatttccga agtaggggta aaatccgtag atctcggaag
      181 gaacaccaaa cgcgaaggca ttctgctggg cacttactga cactgaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008872
VERSION     KHUT01008872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           77 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcctta
       61 taagtcagtg gtgaaatctc tccgctcaac ggggaaactg ccattgatac tgtagggctt
      121 gaatttttgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccgat tgcgaaggca ggttactaac agacgattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008873
VERSION     KHUT01008873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggcgggt
       61 taagtcgaat gttaaagcca gaggctcaac ctctgagatg cattcgatac tggcttgcta
      121 gagggtgata gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacatcagt agcgaaggcg actttctggg tcacacctga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008874
VERSION     KHUT01008874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgc
       61 caagtcggtg gtgaaatctt tcggcttaac cggaaaagtg ccaacgatac tggcggactt
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008875
VERSION     KHUT01008875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          100 g           40 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctggt gtgaaatccc cgggctcaac tcgggacccg cgccagaaac tgccgtgctc
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccaccactga cgctgaggcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01008876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008876
VERSION     KHUT01008876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           91 g           56 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggattta ttgggtgtaa agggtgtgta ggcgggtttg
       61 caagtcagag gtgaaatcct gcggctcaac cgtagaattg cctctgatac tgcaggtctt
      121 gagtcctgga gaggttgtcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccggt tacgaaggtg ggcaactgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008877
VERSION     KHUT01008877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           80 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagt
       61 taagtcagtg gtgaaatctc cgggctcaac ccggaaactg ccgttgatac tatctgtctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaca cagaaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01008878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008878
VERSION     KHUT01008878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggattca ttgggcgtaa agagctcgta ggcggctctg
       61 taagtcggaa gttaaatccc atggctcaac catggacaag ctttcgaaac tgcggagctt
      121 gaggctggga gaggagatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctgga ccagttctga cgctcaggag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008879
VERSION     KHUT01008879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           95 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtctgat gtgaaagccc cgggcccaac ccgggaagtg cattggatac tggcagactt
      121 gaatacggga gagggcagtg gaattcctgg agtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008880
VERSION     KHUT01008880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           96 g           40 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcctgc
       61 taagtcctgc gtgaaatctc ggggctcaac cccgagcggt cgtgggaaac tggcaggcta
      121 gagtgcggga gaggagagtg gaactccagg tgtagcggtg aaatgcgcag atatctggag
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008881
VERSION     KHUT01008881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c           98 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agagcccgca ggcggcttgg
       61 caagtctttg gtgaaatctc ccggcttaac tgggagggtg ccagggaaac tgccaggctt
      121 gaggcaggga gagggatgcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008882
VERSION     KHUT01008882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggcctgt
       61 taagtcgggt gtgaaatctc cgggctcaac ccggaaagtg cattcgaaac tggcgggctg
      121 gaggatggga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ggtccctgga ccattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008883
VERSION     KHUT01008883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           84 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc atggctcaac catggaactg ctcggtatac tggttgtctt
      121 gagccactta ggggctgtcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaaa ggtgaaaaca gacagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008884
VERSION     KHUT01008884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           81 g           57 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta tccggaatta ttgggcgtaa agcgcatgta ggcggtttgt
       61 taagtctggt gtgaaagctc aaggcctaac cttgaaattg cattggaaac tggcaaacta
      121 gaatactgga ggggtaagtg gaattccatg tgtagcggtg gaatgcgtag atatatggag
      181 gaacaccaat ggcgaaggca gcttactgga cagttattga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008885
VERSION     KHUT01008885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggggatg
       61 aaagtcagat gtgaaatccc gaggctcaac ctcggaacag catttgaaac tccatttctt
      121 gaggatagga ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacatcggt ggcgaaggcg gttttctatc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01008886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008886
VERSION     KHUT01008886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 gacggagggc tcaaacgttg ttcggaatca ctgggcataa agagcacgta ggtggcttgc
       61 taagtcgaat gtgaaatccc ccggctcaac cggggaatgg catccgatac tggcaggctc
      121 gaggacggga ggggtaagtg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactggc ccgtctctga cactgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008887
VERSION     KHUT01008887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcaccg
       61 taagtcggat gtgaaagccc ttggcttaac caaggaactg catccgaaac tgcggtgctt
      121 gaatccggaa ggggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggtattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008888
VERSION     KHUT01008888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           92 g           41 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcagag gtgaaatccc ggagctcaac tccggaactg cctccgatac tcccgcgctc
      121 gagggctgga gaggagtccg gaattcacgg tggagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg ggactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008889             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008889
VERSION     KHUT01008889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           59 c           84 g           45 t
ORIGIN      
        1 caccagcgcc acaagtggtc atcgcgatta ttgggcctaa agcgttcgta gccggtttgg
       61 taaatccctt gtgaaattgt tcggcttaac cgaacagcgc gcaggggaca ctgctaagct
      121 cgagaccggg aggggtcaga ggtattccat ggggaccggt aaaatgggat aatccatgga
      181 ggaccaccga tggcgaaggc atctgaccag aacggttctg acggtgagga acgaaagcca
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01008890             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008890
VERSION     KHUT01008890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           54 c           85 g           48 t
ORIGIN      
        1 caccagcgcc tcgagtgggg atcgcgatta ttgggtctaa agcgtccgta gcaggctttg
       61 taaatgcttt gtgaaatcgg caggcataac ctgtcggcgc gcagagtaaa ctgcagaact
      121 cgagaccggg agaggttggc agtattccta gggtaccggt aaaatgggtt aatcctggga
      181 ggactaccga tggcgaaggc agccaaccag aacggttctg actgtgaggg acgaaagcta
      241 ggggagcaaa acgg
//
LOCUS       KHUT01008891             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008891
VERSION     KHUT01008891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta tccggattta ttgggcgtaa agcgtctcgt aggcggtttg
       61 gaaagttgtt tttcaaagac cggggctcaa cctcgggaag ggaggcaata ctaacagact
      121 tggttattgg tggggcttct ggaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaactccga gggcgaaggc aggaagctaa ccaattaacg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01008892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008892
VERSION     KHUT01008892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           83 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggttgtg
       61 taagttagat gtgaaatccc taggctcaac ctgggaactg cgtttaaaac tgcatagcta
      121 gagtactgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctggg cagatactga cactgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008893
VERSION     KHUT01008893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           51 c           81 g           48 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgca ggcggccaag
       61 caagtcagaa gtgaaatccc agggctcaac cctggaattg cttttgaaac tgcatggcta
      121 gaatcccgga gaggttagta gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgtaagcg actaactgga cgggcattga cgctcaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01008894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008894
VERSION     KHUT01008894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggttttg
       61 taagttctgt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcaaggctt
      121 gagtactgga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctctctgga cagtcactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008895
VERSION     KHUT01008895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           90 g           42 t
ORIGIN      
        1 gacgaaggtg gcaaccgttg ctcggattca ctgggcataa agcgcgcgta ggtggctttg
       61 caggtcatcg gtgaaaaacc ccggcacaac cggggaactg ccgatgaaac cgcattgctt
      121 gaggtcggga gaggagagtg gaactcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg gctctctgga ccgatcctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008896
VERSION     KHUT01008896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           89 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggcttgg
       61 taagtcggaa gtgaaattta ggggctcaac ccctaggctg cttccgaaac tgctgagcta
      121 gagtatagga gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt agcgaaggcg actttctgga ctattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008897
VERSION     KHUT01008897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           86 g           49 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agcgcacgta ggcggaaatg
       61 catgtccggg gtgaaatccc ccagctcaac tggggaactg cttcggaaac tgcatttctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggc tcgtatctga cgctgaggtg cgaaagcttg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008898
VERSION     KHUT01008898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacgtagagt gcaaacgttg ttcggaatca ctgggcataa agagctcgta ggcggccgga
       61 aaagtcggtt gtgaaagccc ctggctcaac cagggaattg cagccgaaac tgcttggctt
      121 gagtgtgaga gaggagagtg gaacttccag tggagcggtg aaatgcgtag atattggaag
      181 gaacgccagt ggcgaaagcg actctctggc tcactactga tgctgaggag cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008899
VERSION     KHUT01008899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggttttg
       61 ttagtcgaat gtgaaagccc agggctcaac cttggaagtg catccgatac tgcaaaacta
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cgaagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008900
VERSION     KHUT01008900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgagcgta ggcggcctat
       61 taagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cacacgaaac tggtaggctt
      121 gagtacggga gagggttgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcaacctggt ccgatactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008901
VERSION     KHUT01008901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           85 g           53 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 taagtctatt gtgaaagctc ctggcttaac tgggagaggt caatggatac tgccggactt
      121 gagtatagga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtaactgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008902
VERSION     KHUT01008902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           87 g           55 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggtataa
       61 taagtcagat gtgaaagttc ggggcttaac cccggaattg catttgaaac tgtaatactt
      121 gagtactgga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008903
VERSION     KHUT01008903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           82 g           55 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggattct
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgatac tgggcgtcta
      121 gagtatagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaagcg gcttcctgga ctaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008904
VERSION     KHUT01008904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           40 c           75 g           59 t
ORIGIN      
        1 cacgtaggtc gcgagcgtta tccggaatta ttgggcgtaa agagttcgta ggtggccaag
       61 caagtcttat gtgaaagcca acagcttaac tgttgaattg cataagatac tatttggcta
      121 gaatttagga gaggaaactg gaattccatg tgtagcggtg gaatgcgtag atatatggag
      181 gaacaccgat agcgaaggca ggtttctgga ctaacattga cactaaggaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008905
VERSION     KHUT01008905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcgaaatta ttgggcgtaa agcgcgcgta ggtggtgagg
       61 caagtcctgt gtgaaatcct cgggctcaac ccgagaaagt catgggaaac tacttcgcta
      121 gagtacaaga ggggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctctctggc ttgttactga cactctggcg cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01008906             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008906
VERSION     KHUT01008906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           54 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcaggt gtgaaatctt tgggcttaac ccaaagcgtg cacttgatac tgctgtgact
      121 tgagttcggt aggggagtgg ggaattccta gtgtagcggt gaaatgcgca gatattagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008907             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008907
VERSION     KHUT01008907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c          100 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcgggtcgg
       61 cacgtcggtc gctgaaatcc cccggcttaa ccggggacgt ggcaccgata cggccgacct
      121 ggaggcgagg agggggacct ggaacgtccg gtggagcggt gaaatgcgtt gagatcggac
      181 ggaacgccga cggcgaaagc aaggtcctgg actcgtactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008908             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008908
VERSION     KHUT01008908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           99 g           51 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtcat
       61 taagtgggaa gtgaaaggct ggggctcaac cccatgcagt gcttctcata ctggtggtct
      121 tgagtgcggt agggagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccgg tggcgaaggc ggcttcttgg gccgttactg acgctgatgc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008909
VERSION     KHUT01008909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           94 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggtttgg
       61 taagttggat gtgaaatacc cgggcttaac ttgggggctg catccaatac tgctgaactt
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008910
VERSION     KHUT01008910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctctt
       61 caagtcaggc gtgaaagccc cgagcttaac tcgggaactg cgcttgatac tgtcgagcta
      121 gagtgtggaa ggggcgattg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagg ggcgaaggcg gatcgctggg ccaacactga cgctgatgtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008911
VERSION     KHUT01008911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          103 g           41 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggcgatt
       61 taagttcgtg gtgaaaggcg ggggcttaac ccccgagcgg ccacggaaac tggatcgctt
      121 gagtgtcgga gaggggagtg gaattctcgg tgtagcggtg aaatgcgtag agatcgagag
      181 gaacacccgt ggcgaaggcg gctccctggc cgaacactga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008912
VERSION     KHUT01008912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           83 g           54 t
ORIGIN      
        1 tacgtagggt acaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggggacg
       61 taagtcagat gtgaaatacc aaagctcaac tttggtgctg catttgaaac tgcgtttctt
      121 gaggactgac ggagaaagcg gaattccatg agtagcggtg aaatgcgtag atacatggag
      181 gaacaccggt ggcgaaagcg gctttctagt ttttacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01008913             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008913
VERSION     KHUT01008913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           90 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agggtgtgta ggtggtaggc
       61 ctcgttcgtg gcgacagcca tcggcttaac cgattggagg actgcgaaaa cgggcagact
      121 tgagagcgag agagggacat ggaactccgg gtgcagcagt caaatgcgta gagatccgga
      181 agaacaccaa aggcgaaggc aatgtcctgg ctcgtctctg acgctcaagc acgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008914             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008914
VERSION     KHUT01008914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           51 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggttcgg
       61 taagtcggat gtgaaaactt ggggctcaac cctgagacgc catctgatac tgctgtgact
      121 tgagttcggt agaggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccta cggcgaaggc agcactctgg gccgatactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01008915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008915
VERSION     KHUT01008915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           92 g           43 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccca
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgcgggtctt
      121 gagcggccta ggggcagacg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gtctgctggg ggcctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008916
VERSION     KHUT01008916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           95 g           41 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggctacg
       61 caagtcggac gtgaaacccc gaggctcaac ctcgggactg catccgaaac tgcgtggcta
      121 gaggtaggta ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg actctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01008917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008917
VERSION     KHUT01008917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           85 g           57 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgatg
       61 taagttgagt gtgaaatcct agggcttaac cttagaactg cactcaatac tgcgttgcta
      121 gaggattgta gaggagaatg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg attctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008918
VERSION     KHUT01008918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacggggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggtttta
       61 caagttggat gtgaaagccc tgggcttaac ccaggacgtg cacccaaaac tgtaagactt
      121 gagttccgga gggggttgga gaattcccgg tggagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ccgacctggc cggacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008919
VERSION     KHUT01008919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggcttga
       61 taagtcggat gtgaaatccc tgggctcaac ctgggaactg cattcgatac tgtttggcta
      121 gagtgcggta gaggggggta gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaataccagt ggcgaaggcg gccccctgga ctgacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008920
VERSION     KHUT01008920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtcagag gtgaaatccc acggctcaac cgtggaagtg cccctgaaac tggtcatctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008921
VERSION     KHUT01008921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcgtg
       61 caagtcaggc gtgaaagccc tgggctcaac ccaggaggtg cgcttgatac tgcatgactc
      121 gagtgtggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg accatctgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008922
VERSION     KHUT01008922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggttttg
       61 taagtcggat gtgaaagccc agggcttaac cttggaagtg cattcgaaac tgcataactg
      121 gagtctcgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccggt ggcgaaggcg accacctgga cgaagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008923
VERSION     KHUT01008923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcaggg gtgaaatcct cccgctcaac gggagaacag cttttgatac tggccatctt
      121 gaggggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008924
VERSION     KHUT01008924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggtcgat
       61 gaagtagggt gtgaaatgcc ttggctcaac caaggcacag cgctctaaac tgattgactc
      121 gagcgagcta ggggtatacg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gtatactggg gcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008925
VERSION     KHUT01008925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           86 g           52 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtctga
       61 aaagtctgat gtgaaagccc acagctcaac tgtggaactg cattggaaac tttcagacta
      121 gagtatgaga ggggtaaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg ctttactggc tcattactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008926
VERSION     KHUT01008926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtttga
       61 caagtctgat gtgaaatctt cgagcttaac tcgaaaatgg cattggaaac tgtcgggcta
      121 gaggattgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctaga caattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01008927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008927
VERSION     KHUT01008927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           58 c           81 g           44 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agagcaaaaa gccggtcgca
       61 ttagtctgat gtcaaatcgt ggggcccaac cccatgcctg catcggaaac ggtgtgactt
      121 gagcgggtga gaggtgggcg gaacgtacgg tgtaggggtg aaatccactg atatcgtacg
      181 gaacaccaaa agcgaaggca gcccactagc gctctgctga cggtgcattg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01008928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008928
VERSION     KHUT01008928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggagggt
       61 caagtcggtg gtgaaatcct tccgcttaac gggagcaggg ccgccgatac tgactctctc
      121 gagggaggta ggggcatgtg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008929
VERSION     KHUT01008929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctct
       61 caagtcaggg gtgaaatctt tcggcccaac cggaaaagcg cttctgatac tgggagtctc
      121 gagggaggta ggggtcggtg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg accgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008930
VERSION     KHUT01008930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatgct
       61 taagtcagat gtgaaattcc cgagcttaac ttgggagctg catttgaaac tgggcatcta
      121 gagtgtcgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008931
VERSION     KHUT01008931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           91 g           42 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggatcgg
       61 taagcgtccc gtgaaatccc ccggctcaac cggggaactg cgagacgtac tgccgatctt
      121 gaggaagcta gaggcgggta gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaatgccgaa agtgaagaca gcccgctggg gctttcctga cgctaaagca cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01008932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU8999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008932
VERSION     KHUT01008932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU8999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           92 g           41 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatc
       61 caagtcggcg gtgaaatctt tcggctcaac cggaaaacag ccgccgatac tgggtgcctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008933
VERSION     KHUT01008933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           37 c           90 g           58 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttaga
       61 taagttatct gtgaaagttc agggcttaac cttgagtagt cagatgagac tgtttgactg
      121 gagtatagga gagggtagag gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaggcg tctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008934
VERSION     KHUT01008934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcgggggta
       61 caagtggtgt atgaaaggtc caggctcaac ctggataggt tatgccagac tgtacctcta
      121 gagtacgaga gaggggcatg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gtgccctggc tcgatactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008935
VERSION     KHUT01008935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacagggggt gcaagcgtta ttcggaatta ttgggcgtaa agcgcgcgta ggcggttcgg
       61 taagtttggt gtgaaagccc tgaactcaat tcaggaagtg cattgaatac tgccggactg
      121 gagtactgga gaggagagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacagc agcgaaggcg gctctctgga cagtcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008936
VERSION     KHUT01008936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           86 g           59 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtctgat gttaaagact gaggctcaac ctcggaaatg cattggatac tggcagactt
      121 gagtgtagta gaggcaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgtaagcg gcttgctggg ctataactga cactgaggaa cgaaagccag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01008937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008937
VERSION     KHUT01008937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaataa ctgggcgtaa agggtgcgta gacggtacat
       61 taagctcgtt gttaaattcc ctggcctaac tgggggtaag cggtgagaac tgatgtgcta
      121 gagggtgaga gagagaagtg gaatttccgg agtagcggta aaatgcgtag atctcggaag
      181 gaacaccaat ggcgaaggca gcttcttggc tcatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008938
VERSION     KHUT01008938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           92 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtcaag
       61 taagtcttgt gtgaaatccc tgggctcaac cgaggaagtg catgggaaac tacttggctg
      121 gcagacggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctgga ccgatctgga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008939
VERSION     KHUT01008939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           92 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcgag
       61 taagtcgggt gtgaaagccc tgggcttaac ccaggaagtg caaacgaaac tactcgactt
      121 gagtactgaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008940
VERSION     KHUT01008940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggcggac
       61 taagtacctt gtgaaatccc acggcttaac tgtggaacgg ctgggtatac tggttcgctt
      121 gagcggtcta ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaac ggtgaaaaca gctcgctggg gacctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008941
VERSION     KHUT01008941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           95 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgtt
       61 tgggtgtgat gtgaaagcct tgggcttaac ctgagaagtg catcgcaaac taaatgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008942
VERSION     KHUT01008942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcagg
       61 taagtgggat gtgaaatgcc tcggctcacc cgaggcactg cattccaaac tgcttggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008943
VERSION     KHUT01008943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           64 c          108 g           32 t
ORIGIN      
        1 cacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggcccga
       61 cacatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgcgat ggtcgggctg
      121 gaggcaggga gagggcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctgga cctgacctga cgctgaggcg cgaaggcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008944
VERSION     KHUT01008944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtcgac
       61 taagtttgga gtgacagttt tcggctcaac cgagaaagtg ctctgaaaac tgatcgactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggg gagtacctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008945
VERSION     KHUT01008945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          100 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttgg
       61 caagtccatg gtgaaagctc agggctcaac tctgagtcgg ccacggatac tgcgaagctc
      121 gagtcgacta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg gtcgaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008946
VERSION     KHUT01008946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           96 g           41 t
ORIGIN      
        1 tacggagggg gctagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggtggtccac
       61 caagtcgggg gtgaaagccc agggctcaac cctggaatgg cctccgagac tgatggactc
      121 gagttcggga gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctggc ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008947
VERSION     KHUT01008947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcgg
       61 atagtcggat gtgaaagccc tgggctcaac ctaggaagag cactcgatac taccgagctt
      121 gagttctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008948
VERSION     KHUT01008948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          108 g           36 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggtcctt
       61 taagtccgcg gtgaaagccc ggggctcaac cccggagggt ccgcggagac tggaggactg
      121 gagtgtcgga gaggagcggg gaatccctgg tgtagcggtg gaatgcgtag agatcagggg
      181 gaacaccgat ggcgaaggca ccgctctggc cgaagactga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008949             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008949
VERSION     KHUT01008949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           51 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 caagtcaggt gtgaaaactc taggctcaac ctggagacgc cacttgatac tgctatggct
      121 tgagttcggt aggggatcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgatctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01008950             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008950
VERSION     KHUT01008950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           64 c           96 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatcgg
       61 aacgtcggcg cctgaaatcc cccggctcaa ccggggaacg cggcccgaaa cgaccgatct
      121 cgaggggtgt aggggggact ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagtccctgg acgcctgctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01008951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008951
VERSION     KHUT01008951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           92 g           42 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtctcg
       61 aaagcccacc gtgaaagccc ttggcccaac tgagggaggc cggtgggaac ttcgagactt
      121 gaaggcggga gaggcagatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaggcg atctgctggc ccacccttga cgctcaggcg cgaaagctag
      241 ggtagcgacc ggg
//
LOCUS       KHUT01008952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008952
VERSION     KHUT01008952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcgg
       61 aaagtcgggt gtgaaatccc tgggctcaac ccaggaactg catccgaaac ttccggtctt
      121 gagtgacgga gagggaggta gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcctcctgga cgtccactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008953
VERSION     KHUT01008953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          104 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggaagct
       61 taagcgcggc ctgaaagccc ccggctcaac cggggagggc ggtcgcgaac tgggcttctt
      121 gagggcgaca gaggagcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcgctctggg tcggctctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008954
VERSION     KHUT01008954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggcggcc
       61 atcgtcggat gtgaaattcc tgggcttaac ccgggggctg cattcgatac gtggtcgctg
      121 gagtgcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008955
VERSION     KHUT01008955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaacag ccttggatac tggcggcctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctccactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008956
VERSION     KHUT01008956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggttttt
       61 taagtgggat gtgaaatccc cgggcttaac ccgggaaccg catttcaaac tggaagacta
      121 gagtgcagga gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggct cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008957
VERSION     KHUT01008957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           98 g           39 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtaa agggcacgta ggtggcacga
       61 caagaccggt gtgaaatccc acggcttaac cgtggggcgc cactggtgac tgtcgcgctc
      121 gagcctggca gaggtgggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggggaaccca gcccactggg ccagtgctga cactgaggtg cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008958
VERSION     KHUT01008958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcggca
       61 caagtcaggt gtgaaatccc ccggctcaac cggggaacag cgcttgaaac taggtcgctc
      121 gagtgaggca ggggtatgtg gaacttccgg tggagcggtg aaatgtgtag atatcggaag
      181 gaacgccggc ggcgaaagcg gcatactggg ccttgtctga cgctgaggca cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01008959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008959
VERSION     KHUT01008959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggtgaat
       61 taagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tgattgactg
      121 gagtacaaga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacgtcgat ggcgaaggca gccacctggc ttgatactga cactgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01008960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008960
VERSION     KHUT01008960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           83 g           61 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggatttt
       61 taagtcaggg gtgaaatttt tcggctcaac cggaagcttg cctttgatac tgaagatctt
      121 gaatgtcgtt gaggacggcg gaatgggaca agtagcggtg aaatgcatag atatgtcccg
      181 gaaccccgat tgcgaaggca gctgtccaag cgatgattga cgctgatgca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01008961             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008961
VERSION     KHUT01008961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           48 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggcttcg
       61 taagtctcat tgtgaaagcc ctcggctcaa ccgaggaact gcatgagaaa ctgcgaggct
      121 tgagttcggg agaggagagt ggaattccca gtgtagcggt gaaatgcgta gatattggga
      181 ggaacaccgg tggcgaaggc ggctctctgg accgatactg acgctgagac gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01008962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008962
VERSION     KHUT01008962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatca ttgggcgtaa agcgcttgta ggcggcctga
       61 aaagtctcgt gtgaaatccc ctggcttaac cagggaagtg cgcgagaaac ttttgggctt
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008963             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008963
VERSION     KHUT01008963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          101 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa aaggcttgta ggtggccacg
       61 gcaagtcgga tgtgaaatct cccggctcaa ctgggagggg tcattcgata ctgccgggct
      121 cgaggccggt agggggaagc ggaattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag aggcgaaggc ggcttcctgg accggtcctg acactgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01008964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008964
VERSION     KHUT01008964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           78 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtat agggcgcgta ggcggctcta
       61 caagttggat gtgaaatcct agggctcaac cttagaacag catccaaaac tgtcgagcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttattcctaa cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01008965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008965
VERSION     KHUT01008965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggtttgg
       61 taagttggaa gtgaaatccc ggggctcaac ctcggaattg ctttcaaaac tgccaatcta
      121 gagtatggta ggggacagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccaga ggcgaaggcg actgtctggg ccattactga cgctaaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008966
VERSION     KHUT01008966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           88 g           48 t
ORIGIN      
        1 tacgaactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgat
       61 caggtccgat gtgaaatccc acagctcaac tgtggaacgg cattggaaac cggttggctt
      121 gagtaaacta gagatgtacg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg gtacattggg gtttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008967
VERSION     KHUT01008967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatca ttgggcgtaa agcgagcgca ggcggattga
       61 taagtcaggt gtgaaatctc gaagctcaac ttcgaaactg cgcctgaaac tattagtcta
      121 gaatgtcgga gggggcaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggg ggcgaaggcg cctgcctgga cgactattga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008968
VERSION     KHUT01008968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcccgca ggcggttcgc
       61 caagtttggg gtgacagctc ctggcttaac caggagagtg ctgcaaaaac tggtggactt
      121 gagagcgaga gagggacatg gaactccggg tggagcagtg aaatgcgtag atatccggaa
      181 gaacaccaaa ggcgaaggca atgtcctggc tcgtttctga cgctcagggg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008969
VERSION     KHUT01008969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggacagt
       61 ttagtcagag gtgaaagccc ggggctcaac ctcggaatag cctttgatac tggctgtctt
      121 gagtatggaa gaggtatgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acatactggt ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008970
VERSION     KHUT01008970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggattta ctgggtgtaa agggcgcgta ggcgggaatg
       61 caagtcagat gtgaaatcca agggctcaac ccttgaactg catttgaaac tgtatttctt
      121 gagtgtcgga gaggttgacg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gtcaactgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008971
VERSION     KHUT01008971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           61 c          108 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcagtc
       61 ggagcccggc gtgaaagctc ccagcttaac tgggagaggt cgtcggggac ccggcagctc
      121 gaggccggta ggggtgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gctcactggg ccggtcctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01008972             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008972
VERSION     KHUT01008972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           81 g           61 t
ORIGIN      
        1 tacgaagggt gcaagcgttg cccggaatca ttgggcgtaa agtgtttcgt aggcgttctt
       61 gtaagttatt ctttaaagcc tccggcttaa ccggagaaag gggagtaata ctgcaagaat
      121 tgaagaatat cagggtaact ggaattgtcg gtgtaggggt gaaatccgtt gatctcgaca
      181 agaactccaa aggcgaaggc gggttactgg gtattatttg acgctgatga acgaaagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01008973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008973
VERSION     KHUT01008973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          107 g           33 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtcgcg
       61 taagtccgct gtgaaagccc ggggctcaac cccggcacgt cagcggagac tgtgcgactg
      121 gggggtcgga gaggagccgg gaatccctgg tgtagcggtg gaatgcgtag agatcagggg
      181 gaacaccgac ggcgaaggca cggctctggc cgacatccga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008974
VERSION     KHUT01008974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           50 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggtggttctc
       61 caagtctggt gtgaaatctc ctggctcaac tgggagggtg cgccggaaac tggagaactt
      121 gagtgtggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagacg gctttctgga ccacgactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008975
VERSION     KHUT01008975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c          100 g           37 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatta ctgggcgtaa agcgctcgta ggcggtcgca
       61 tacggcggtg gtgaaagctc ccggctcaac tgggagaggc ccgccgcaac ggtgcgactc
      121 gaggatggta gaggcagatg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gtctgctggg ccacacctga cgctgaggag cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008976
VERSION     KHUT01008976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           96 g           38 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgtgtgta ggcggtcccg
       61 cacgtcgggt gtgaaagccc ggggctcaac cccggaagcg catccgctac gacggggctg
      121 gagtgtcgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgcaggcg gctctctgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008977
VERSION     KHUT01008977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           92 g           41 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaaagg ctgggcagac tggcggtctt
      121 gagctgtcca ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggcgaaagca ggttgctgga gacaagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008978
VERSION     KHUT01008978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           94 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctatc
       61 taagtctggt gtgaaagccc tgggctcaac ccgggaagtg cactggatac tggatagcta
      121 gaggacgaga gaggagagtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gctctctggc tcgtatctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008979
VERSION     KHUT01008979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 tacgtacgag gcaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcggatgtt
       61 taagtagggc gtgaaatctc ccggcctaac tgggaggggt cgctctagac tgggcgtctt
      121 gaggactgca gaggtgggtg gaattcctgg tgtagtggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcccactggg cagtcactga cgctgaggcg cgaaagctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008980
VERSION     KHUT01008980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           88 g           54 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggatctt
       61 caagtgcctt gtgaaatccc atggctcaac catggaattg ctcggtagac tggggatctt
      121 gaggtaggta ggggtcactg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgat ggtgaagaca ggtgactggg cctattctga cgctgatgca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008981
VERSION     KHUT01008981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgtctt gtgaaatccc ccggctcaac cggggaatgg ctgggcatac tggcgatctt
      121 gagcagtcta ggggcagatg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaaa ggtgaagaca gtctgctggg gacttgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008982             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008982
VERSION     KHUT01008982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           96 g           43 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtttct
       61 taagtccttt gtgaaaatcc ggagctcaac tccgcgaaat gcagaggata ctgggagact
      121 agagaccggt agaggctggc ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacaccgg tggcgaaggc ggccagctgg gccgggtctg acgctcaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01008983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008983
VERSION     KHUT01008983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccttg
       61 caagtcggat gtgaaagccc tcggctcaac cggggaagtg cacccgaaac tgcaaggctc
      121 gagttccgga gaggatagtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008984
VERSION     KHUT01008984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           90 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggttttt
       61 taagttagat gtgaaaaccc agggcttaac tttgggcgtg catttaaaac tggagagctt
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01008985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008985
VERSION     KHUT01008985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 gacagggggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggtctcg
       61 taagtcgggt gtgaaatccc acagcttaac tgtggaactg cgcccgatac tgcggggctt
      121 gaggatggga ggggagagcg gaattccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccattcctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008986
VERSION     KHUT01008986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcgg
       61 taagtctggt gtgaaatcca ggggctcaac ccctggcggt cactggatac tgtcgggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008987
VERSION     KHUT01008987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacggaggag gcgagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggtggctcgg
       61 taagtatctt gtgaaatccc ccggcttaac cgaggaatcg caaggtatac tgccaggctt
      121 gaggcggata ggggtgcctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcccgt ggcgaaagcg gggcactggg tccgatctga cactgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01008988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008988
VERSION     KHUT01008988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggcggta
       61 tatgtgtggt gtgaaagccc cgggcttaac ctgggaagtg caccgcaaac tgtacggcta
      121 gagtatatga gagggcggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccgcctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01008989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008989
VERSION     KHUT01008989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           53 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggtcttg
       61 ttagtcttct gtgaaattct tcggcttaac cgggggtccg caggggaaac ggcaggacta
      121 gaggatgcga gaggtgtgcg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca gcacactggc gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01008990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008990
VERSION     KHUT01008990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           93 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggcagct
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaaatag cctccgatac tgggccgctc
      121 gagggagaca ggggcacgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga tctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01008991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008991
VERSION     KHUT01008991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 gacagaggcc tcgagcgtta gacggaatca ctgggcttaa agcgtgtgta ggcggaaggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgcttttctt
      121 gagcaatcta ggggcgagtg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgaagaca actcgctggg gatttgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008992
VERSION     KHUT01008992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           83 g           59 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgta ggcggtctta
       61 taagtcagag gtgaaatcct gcagcttaac tgcagagctg cctttgatac tgtaagactt
      121 gagttcggaa gagagagata gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaataccagt tgcgaaggcg atctcttggt ccgatactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01008993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008993
VERSION     KHUT01008993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           87 g           52 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccttt
       61 caagtcaggt gtgaaatccc acggcccaac cgtggaactg cgcttgaaac tgtttggctt
      121 gagtcagcta gaggtacacg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaggcg gtgtactggg gctgttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008994
VERSION     KHUT01008994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ctgggcgtaa agcgcacgca ggcggcaagg
       61 tgggtagggc atgaaatctc ctggcttaac tgggaggcgc tgtcctatac cgcctagctt
      121 gaggatagga gagggaagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctatacctga cgctcaagtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01008995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008995
VERSION     KHUT01008995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           55 c          110 g           41 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggctgat
       61 caagcccgac gtgaaagccc ccggcttaac cggggagggt cgttggggac tggttggctt
      121 gagggcggga gaggttggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccgactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01008996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008996
VERSION     KHUT01008996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           98 g           42 t
ORIGIN      
        1 aacgtaggag acgaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtcgtt
       61 taagttcggc gtgaaagcct ccggctaaac cgggggaggt cgtcggaaac tggatgacta
      121 gagggtgata gaggcaggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acctgctggg tcacacctga cgctgagacg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008997
VERSION     KHUT01008997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           94 g           51 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggattca ctgggcttaa agggtgcgta ggcgggtgat
       61 caagtcaggg gtgaaatcct cccgcttaac gggagaagtg cctttgatac tggtcgtctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01008998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008998
VERSION     KHUT01008998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           75 g           55 t
ORIGIN      
        1 tacgaggaga gcaagcgtta tccggattta ttgggcgtaa agcgtgcgca agcggtttta
       61 taagtgagat gttaaatacc aaggctcaac cttggaatcg cacctcatac tgtaagacta
      121 gagtcaatga gaggcaaacg gaattgctgg tgtagtagtg aaatgcgttg atatcagcag
      181 gaacaccaaa agcgaaagca gtttgctggc attgtactga cgccgttgca cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01008999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01008999
VERSION     KHUT01008999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           98 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggtcatc
       61 taagtcaggt gtgaaagccc ccggctcaac tggggagggt cacttgatac tggatgactt
      121 gagtgcggga gagggaagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgtaactga cgctgaggcg cgaaagctgg
      241 gggagtgaac ggg
//
LOCUS       KHUT01009000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009000
VERSION     KHUT01009000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggttgattgg
       61 taagtcagtg gtgaaatact ccgactcaat cggagaactg ccgttgatac tatcaatctt
      121 gaatatggtg gaggcgggcg gaatgcgtca tgtagtggtg aaatacttag atatgacgca
      181 gaacacccat tgcgaaggca gctcgctacg ccattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009001
VERSION     KHUT01009001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           85 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctatt
       61 caagtcaggg gtgaaatcct tgggctcaac ccaagaattg cccttgaaac tgtatagcta
      121 gagtgtcgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcacg
      241 gggatcaaac agg
//
LOCUS       KHUT01009002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009002
VERSION     KHUT01009002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcggt
       61 aaagtcaggg gtgaaatcct ccagctcaac tggagaagtg cctctgatac tgatcgtctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009003
VERSION     KHUT01009003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcgggacgg
       61 aaggtgcttg gtgaaatccc ttggctcaac caaggaactg ccgggcaaac catcgtcctt
      121 gaggcaggta gaggtcgctg gaaccttagg tggagcggtg aaatgcgtag agatctaagg
      181 gaacgccggt ggcgaaagcg agcgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01009004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009004
VERSION     KHUT01009004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggaggtcgcg
       61 taagtcggtt gtgaaatccc tgggctcaac ctgggaattg cttccgatac tgcgggacta
      121 gagttcggta gagggtggcg gaactcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccgat ggcgaaggca accacctggg cctgaactga ctctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009005
VERSION     KHUT01009005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c           98 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatggc
       61 tttgtcgggc gtgaaattcc agggctcaac cttgggactg cgttcgagac gggttgtcta
      121 gagtttggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009006
VERSION     KHUT01009006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta gtcggtattg
       61 agagtcacgg gtgaaatccc caagcttaac ttgggaactg cctgtgagac ctcagtacta
      121 gagtgtgaga ggggatagtg gaatgccaag tgtagcggtg aaatgcgtag agatttggcg
      181 gaacaccggt ggcgaaggcg gctatctggc tcacaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009007
VERSION     KHUT01009007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          104 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccag
       61 ccagtctggg gtgaaagccc ccggctcaac cggggaacgg cctgggatac tgctggcctg
      121 gaggggggta ggggcgcctg gaacactggg tggagcggtg aaatgcgttg atatccagtg
      181 gaactccggt ggcgaaagcg aggcgctggg ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009008
VERSION     KHUT01009008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggttgg
       61 taagccagat gtgaaatccc tatgctcaac gtaggaactg catttggaac tgccagtctt
      121 gagtagtgga gaggaaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gctttctgga cactaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009009
VERSION     KHUT01009009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta gtcggaatta ctgggcgtaa agcgtgcgca ggtggttatt
       61 taagtcaggt gtgaaagccc tgggctcaac ccgggaattg cgcttgaaac tagatagcta
      121 gagtttagca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaaaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009010
VERSION     KHUT01009010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           97 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggtctct
       61 taagtcttgt gtgaaaggcc acggcttaac cgtggagagc cacaggaaac tgggagacta
      121 gagtgtcgga gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cgataactga cgctgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01009011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009011
VERSION     KHUT01009011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcat
       61 caagtcaggg gtgaaatccc acggctcaac cgtggaagtg cctttgatac tggtggtctg
      121 gagtggggta ggggcatgtg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccccaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009012
VERSION     KHUT01009012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggttttg
       61 taagtcgaat gtgaaagccc agggctcaac cttggaagtg catccgaaac tgcgaaactt
      121 gaatcctgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccggt ggcgaaggcg accgcctgga cagagattga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009013
VERSION     KHUT01009013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggttcc
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaatag cctcggatac tgggagtctt
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009014
VERSION     KHUT01009014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttatc
       61 taagtcaggt gtgaaatctc tcggctcaac cgggaaactg cgcttgaaac tgggtgactg
      121 gagggatgca ggggtgtgtg gaacttccgg tggagcggtg aaatgtgtag atatcggaag
      181 gaacgccagc agcgaaagcg gcacactggg catctactga cgctgaggca cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01009015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009015
VERSION     KHUT01009015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggagcgta ggtggcgtcg
       61 taagtcaggt gtgaaatctc ggggctcaac cccgaaactg cacccgatac tgcgatgcta
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cactgaggct cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009016
VERSION     KHUT01009016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcagttttg
       61 catgtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgcaagactg
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009017
VERSION     KHUT01009017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           97 g           39 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactta ctgggcgtaa agcgcacgca ggcggtcaca
       61 ggcgtcggtg ctgaaagcgc accgcttaac ggtgcaacgc gtgccgatac gctgtgactg
      121 gagccatgca gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca gcggactggg catcggctga cgctcatgtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01009018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009018
VERSION     KHUT01009018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           91 g           42 t
ORIGIN      
        1 gacaggggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctcct
       61 taagtccgct gtcaaatccc agggctcaac cctggacagg cggtggaaac tactaagctg
      121 gagtacggta ggggcagagg gaatttccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaaa ggcgaaggca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01009019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009019
VERSION     KHUT01009019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcgggtccg
       61 caagtgcctt gtgaaatccc acggctcaac cgtggaatgg ctcggcagac tgcgggtctt
      121 gaggtcggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca agcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009020
VERSION     KHUT01009020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgta
       61 taagtgggtt gtgaaatccc tgggcttaac ctgggaacgg caatccagac tgtatgacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacacccgt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009021
VERSION     KHUT01009021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           88 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcttt gtgaaatccc ctggctcaac cagggaactg ctcggcatac tggcgatctt
      121 gagataccta ggggcaactg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaat ggtgaagaca ggttgctggg ggtattctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009022
VERSION     KHUT01009022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           52 c           87 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaccga
       61 taagtcaggc gtgaaatccc cgagctcaac tcgggaactg cacctgaaac tgccggtcta
      121 gagtgatgga gagggtagcg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg actacctgga cattaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009023
VERSION     KHUT01009023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttata
       61 taagttggat gtgaaaaccc tgggcttaac ctaggaattg catacaagac tgtatgactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009024
VERSION     KHUT01009024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           88 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgcgcgta ggcggcttac
       61 taagtctgat gtgaaagccc tggactcaat tcaggaagtg cattggatac tggtaggcta
      121 gagtatagga gaggtgagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg actcactgga ctataactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009025
VERSION     KHUT01009025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           77 g           57 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggtcttg
       61 taagtcagtg gtgaaatctc gcagcttaac tgcgaaactg ccattgatac tgcaggactt
      121 gagtacagat gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggct cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009026
VERSION     KHUT01009026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtaggta ggtggtctcg
       61 tttgtctagg gtgaaagccc agggcttaac cctggaagtg ccctagaaac ggcgagacta
      121 gagtattgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caattactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009027
VERSION     KHUT01009027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcgcga
       61 aaagtctggg gtgaaatctt tcggcttaac cggaaaagag cccccgatac tggcgcgctg
      121 gagggaggta ggggcatgtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttctctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009028
VERSION     KHUT01009028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaagccc tgggctcaac ctaggaagtg cactcgaaac tgcctcgctt
      121 gaatgccgga gaggaaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctttctgga cggttattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009029
VERSION     KHUT01009029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggtggcccga
       61 tcagtccgct gtgaaagtcc agggctcaac cctggaaagc cggtggatac tgtcgggcta
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gcactctggg acggtactga cactgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009030
VERSION     KHUT01009030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           94 g           50 t
ORIGIN      
        1 gacggaggtc tctagcgttg ttcggaatta ctgggcttaa agcgcgtgta ggcggggtgg
       61 caagtaccgt gtgaaatccc tgggctcaac ccgggaacgg ctcggtatac tgtcactctt
      121 gaggcaggta ggggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01009031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009031
VERSION     KHUT01009031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           87 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtttga
       61 aaagtcttct gtgaaatcta cgggcttagc ctgtaaagtg cagaggatac tatcaggctt
      121 gagtatcgga gaggataggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctatctggc cgataactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009032
VERSION     KHUT01009032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c           99 g           50 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggcctgt
       61 cgcgtcgatc gtgaaaactg ggggcttaac ttccagcttg cggtcgatac gggcaggctt
      121 gaggtaggta ggggagatcg gaattccacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctatcctga cgctgatgag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009033
VERSION     KHUT01009033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggtggtcttg
       61 caagtcaggg gtgaaagccc agagctcaac tctggaactg cccttgagac tgtgagactt
      121 gggtacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgttaccga cactcaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009034             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009034
VERSION     KHUT01009034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtggg
       61 gacgtcggca tctgaaagcc cccggctcaa ccggggaagg ggatccgaaa cgccccgcct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctgctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01009035             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009035
VERSION     KHUT01009035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           99 g           43 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gtaagtcgga agtgaaattc caccgctcaa cggtggcgct gcttccgata ctgcggatct
      121 ggggaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccgggtccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009036
VERSION     KHUT01009036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           89 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatga ctgggcgtaa agcgcatgta ggcggcctag
       61 caggtcggat gtgaaatccc tcggctcaac caaggaagtg cattcgaaac cgcaaggctt
      121 gagtcctcga gaggaaggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaagcg gccttctgga gagtgactga cgctgagatg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009037
VERSION     KHUT01009037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           58 c          110 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccccg
       61 taagtcggcc gtgaaatctc cgggcttaac tcggaggggt cgggcgagac tgcggggctg
      121 gaggcgggta gagggcagtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctgcctggg cccgacctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01009038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009038
VERSION     KHUT01009038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           97 g           50 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtctag
       61 tgtgtctgcc gtgaaattgc atggctcaac tgtgccaagt cggtagaaac tgcaagactt
      121 gaggttggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacacttgt ggcgaaggcg gccagctgga ccaatcctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009039             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009039
VERSION     KHUT01009039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatga ctgggcgtaa agggtgtgta ggcgggctcg
       61 caagttggat gtgtaatacc cagagcttaa ctcgggtgct gcatctgaaa ctacgagtct
      121 tgagtgtcgg agaggtaagt ggaattccta gtgtagcggt ggaatgcgta gatattagga
      181 ggaacatcag tggcgaaggc gacttactgg acgataactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009040
VERSION     KHUT01009040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           93 g           41 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcccgg
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaatgg cgcccaaaac tgccgggctg
      121 gagggagaca gaggtgagcg gaacagcggg tggagcggtg aaatgcgttg atatccgctg
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009041
VERSION     KHUT01009041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctctt gtgaaagctc ccggcttaac tgggagaggc caagggaaac taccgggctt
      121 gagtgtggta gaggaagcta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctgga ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009042
VERSION     KHUT01009042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           94 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgac
       61 caagtcaggg gtgaaatcct ccggctcaac cggagaacag cctttgatac tggtcacctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009043
VERSION     KHUT01009043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcttt
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaattg cctcggatac tgggagtctg
      121 gagggaggta ggggcgagcg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccggt ggcgaaagcg gctcgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009044
VERSION     KHUT01009044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          101 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagtctgtg gtgaaagccc ccggcttaac cggggaagtg ccgcggatac tggcggtctc
      121 gaggggtgca ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cacctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009045
VERSION     KHUT01009045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           97 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggctttg
       61 taagttaggc gtgaaaggcc tgggcttaac ccgggaatgg cgcttaagac tgcggagctt
      121 gaggatggga ggggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccaat ggcgaaggcg gccatctgga ccatcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009046
VERSION     KHUT01009046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           75 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggatttt
       61 taagtcagtg gtgaaagcct acagcttaac tgtagaactg ccattgatac tgaaagtctt
      121 gagtacatat gaagtgggcg gaatgtgcag tgtagcggtg aaatgcttag atattgcaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009047
VERSION     KHUT01009047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctctg
       61 taagtggtat gtgaaatccg atggctcaac catcgaactg cgttccatac tgcagagctt
      121 gagtacagca gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccgtt ggcgaaggcg actttctggg ctgctactga cgctgatgcg cgaaagccag
      241 gggagcgaac agg
//
LOCUS       KHUT01009048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009048
VERSION     KHUT01009048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           84 g           55 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggttaga
       61 taagtctggc attaaagact ggagctcaac ttcaggaatg taccggaaac tgtctgacta
      121 gaggacaata gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg gacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009049
VERSION     KHUT01009049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggccttg
       61 tcagtccggt gtgaaagccc agggctcaac cctggaagtg catcggatac tgcaaggctg
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009050
VERSION     KHUT01009050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           83 g           57 t
ORIGIN      
        1 tacgagtgcc tcaagcgtta tccggaatca ttgggcgtaa aggttgtgta ggtggttgta
       61 ttagtcttct gttaaattct tcggcttaac cgggggcatg caggggaaac ggtacaacta
      121 gaggatgcga ggggtctgcg gaactcatag tgtagcggtg aaatgcgttg atattatggg
      181 gaacaccaaa agcgaaggca gcagactgga gcactcctga cactgaaaca agaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01009051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009051
VERSION     KHUT01009051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggcctac
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgaaac tggtgggctt
      121 gaatgtcgga gagggcagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actgcctgga cgaacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009052
VERSION     KHUT01009052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           92 g           47 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggtgggttgg
       61 ccagtctatg gtgaaatcct ccggcttaac cggagaactg ccgtggatac tgccagtctc
      121 gaggagggta ggggcgtctg gaactagcgg tggagcggtg aaatgcgttg atatcgctag
      181 gaactccggt ggcgaaggcg agacgctgga ccctttctga cactgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009053
VERSION     KHUT01009053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctagg
       61 taagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcttggctt
      121 gagtgccgga gaggatggcg gaatacccca agtagaggtg aaattcgtag atatggggtg
      181 gaacaccggt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009054
VERSION     KHUT01009054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           89 g           46 t
ORIGIN      
        1 tacaggggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggaccag
       61 taagcgcttt gtgaaatccc ccggctcaac cggggaattg cagggcgaac tgctggtcta
      121 gaggaaacta gaggcgggca gaacgattgg tggagcggtg gaatgcgtag atatcaatcg
      181 gaatgccgaa agtgaagaca gcccgctggg gttttcctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01009055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009055
VERSION     KHUT01009055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           94 g           47 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggtggtttta
       61 taagtcactg ttgaaagctc ccggctaaac tgggagaggt gcggtgagac tgtagagcta
      121 gagtttggga gaggggtgcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcaccctggc ctaatactga cactgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009056
VERSION     KHUT01009056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           85 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggaccag
       61 caagtcaggc gtgaaatccc taggctcaac ctaggaactg cacctgaaac ttctggtctt
      121 gagtggtgga gaggaaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cacttactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009057
VERSION     KHUT01009057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcgggagtt
       61 taagtcgtat gtgaaagccc cgggcttaac ctgggaatgg catatgagac tgggcaccta
      121 gagtagggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ttcatactga cgctgaggcg cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009058
VERSION     KHUT01009058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          101 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtacgg
       61 caagtgggat gtgaaagccc tgggctcaac ctgggaactg catcccggac tgctgtgctg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctgcctgga cagatactga cgctggggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009059
VERSION     KHUT01009059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           84 g           57 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttga
       61 taagttggat gtgaaagccc tgggctcaac ccgggaattg cattcaatac tattttacta
      121 gaatttggta gaggttggta gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaatacccgt ggcgaaggcg gccaactgga ccaacattga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009060
VERSION     KHUT01009060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           78 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtaaag
       61 taagtcagtg gtgaaagttt gcagcttaac tgtaaaattg ccattgatac tgcattgctt
      121 gagtccagat gaggtgggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctggcactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009061
VERSION     KHUT01009061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtctga
       61 caagtcggac gtgaaagccc cgggctcaac ccgggaagtg cgctcgatac tgccagactt
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009062
VERSION     KHUT01009062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           87 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgtatgta ggcggttttc
       61 taagtctgat gtgaaatccc aaggcttaac cttggaactg cattggatac tggaaagcta
      121 gaggatggta gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccatctgga ccatgtctga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009063
VERSION     KHUT01009063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           84 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatatt
       61 taagtcggat gtgaaagccc tgggcttaac ctaggaattg catccgatac tggctatctc
      121 gagtatggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gctacctgga ccaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009064
VERSION     KHUT01009064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           62 c          109 g           33 t
ORIGIN      
        1 cacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccacg
       61 cacatcaccg gtgaaagccc cgggcttaac ccgggagggt ccggtgagat ggcgtggctg
      121 gaggcagggc gaggccggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccggctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009065
VERSION     KHUT01009065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           90 g           48 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctcgg
       61 taagttgggt gtgaaatccc acggctcaac cgtggaattg cgctcaaaac tgtcgagctt
      121 gagggaggta gaggtgagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gctcactggg cctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009066
VERSION     KHUT01009066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgtt
       61 caagtcaggt gtgaaatccc ccggctcaac cggggaatgg cgcttgaaac tgggcggctg
      121 gagtcagcta gaggtccgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaagcg gcggactggg gctgtactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009067
VERSION     KHUT01009067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           79 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggcctgt
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cccttgatac tgatgggctt
      121 gaatccactt gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaaa gtggtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009068             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009068
VERSION     KHUT01009068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           96 g           43 t
ORIGIN      
        1 tacgggggga gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttag
       61 aaagtctact gtcaaagacc agggcttaac cccgggaagg cagtggagta cttctaggct
      121 agagtgcggt agaggcagag ggaattccgc gtggagcggt gaaatgcgta gagatgcgga
      181 agaacaccag tggcgaaggc gctctgctgg gccgagactg acactgagag acgaaagcta
      241 ggggagcgaa aagg
//
LOCUS       KHUT01009069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009069
VERSION     KHUT01009069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agggttcgta ggcggcgttg
       61 taagtgagat gtgaaatccc tcggctcaac cgaggaactg cgtttcaaac tacaatgctt
      121 gagggagaca ggggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009070
VERSION     KHUT01009070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           87 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggttatg
       61 taagtcagag gtgaaaggtc acagcttaac tgtgagatgg cctttgaaac tgtgtaactt
      121 gaatcagata gaggttagtg gaatgcgaca tgtagcggtg aaatgcatag atatgtcgtg
      181 gaataccgat tgcgaaggca gctaactggg tctggattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009071
VERSION     KHUT01009071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcgggt gtgaaagacc ggggctcaac tccggttctg cattcgatac gggcaggcta
      121 gagtgtggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009072
VERSION     KHUT01009072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           86 g           57 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggctgttagt
       61 taagtcagat gtgaaagccc tcggctcaac cggggaagtg catttgaaac taactggcta
      121 gaagacggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaatacctgt ggcgaaggcg gccttctgga ccgttcttga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009073
VERSION     KHUT01009073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttacg
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaattg cattcgagac tgcatagctt
      121 gagtgcggaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctggt ccagcactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009074             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009074
VERSION     KHUT01009074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           98 g           46 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggacgtg
       61 tgtgtctggc gtgaaatctc cactgcttaa cagggagagg ccgctggaaa ctgcacgtct
      121 ggagtttggc agaggaatgt ggaattccgc atgtagcggt ggaatgcgta gatatgcgga
      181 agaacaccgg tggcgaaggc ggcattctgg gccaaaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009075
VERSION     KHUT01009075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agggctcgta ggtggtttgg
       61 taagtcgttc gtaaaagctc ggggctcaac cccgagatgc ggtcgaaact gccatgacta
      121 gggtccggta gaggtgagtg gaattctcgg tgtagcggtg gaatgcgcag atatcgagag
      181 gaacaccagt agcgaaggcg gctcactggg ccggtaccga cactgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009076
VERSION     KHUT01009076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgatc
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cattcgatac tgggttgctt
      121 gagtaccgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009077
VERSION     KHUT01009077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           81 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcttg
       61 taagtccgtg gtgaaatctc tgagcttaac tcggaaactg ccatggatac tatgggtctt
      121 gaatactcgg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca gagttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009078
VERSION     KHUT01009078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           84 g           47 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcacgg
       61 taagtggggt gtgaaatccc tcagctcaac tgaggaactg cgctccaaac taccgtgctt
      121 gagtgagaca gaggttagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gctaactggg tctcaactga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009079
VERSION     KHUT01009079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           97 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggacggg
       61 taagttgggt gtaaaagctc ctggctcaac tgggagaggt cgctcaagac tgccggtctg
      121 gagcgtggga gaggaaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccacgactga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009080
VERSION     KHUT01009080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatca ctgggcttaa agggtgtgta ggcggtttgg
       61 taagcatcgt gtgaaagccc caggctcaac ctgggaattg cacgatgtac tgcctgactt
      121 gaggcgtgta gaggttaccg gaactctggg tggagcggtg aaatgcgtag atatccaggg
      181 gaacgccaat ggcgaaggca ggtaactggg cacgtcctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009081
VERSION     KHUT01009081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           54 c           80 g           45 t
ORIGIN      
        1 tacagagacc tcaagcatta tccggattta ttgggcgtaa agcgtccgca ggcggcacgt
       61 taagtctctc gtcaaatcta tgggctcaac ccataggctg cgagggaaac tggcgagcta
      121 gaggttggga gaggcaagcg gaattaccgg tgtagtagta aaatgcgtta atatcggtaa
      181 gaacaccaaa ggcgaaggca gcttgctgga acacacctga cgctaaggga cgaaagcacg
      241 gggagcgaag cgg
//
LOCUS       KHUT01009082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009082
VERSION     KHUT01009082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggatacc
       61 taagtcgaat gtgaaatccc cgggcttaac ctgggaactg catccgatac tgggtatctg
      121 gagtatggta gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg actttctgga ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009083
VERSION     KHUT01009083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctgcc
       61 taagtctggt gtgaaagccc gaggcttacc ctcggaagcg cactggaaac tgggcagctc
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009084
VERSION     KHUT01009084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           98 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggcgcgca ggcggttcgt
       61 taagtccgag gtgaaatctc ccggctcaac tgggagaggt cttcggatac tggcggactg
      121 gaggtgagca gaggtgagtg gaattctcgg tgtagtggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gctcactggg ctctacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009085
VERSION     KHUT01009085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggccgca
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaatgg cgcttgtgac tgtgtggcta
      121 gagttcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcgcgactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009086
VERSION     KHUT01009086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           82 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggattga ttgggcgtaa agggtgcgca ggcggtatgt
       61 taagtcaact gttaaatatc ccagcttaac tgggaaacag cagtagatac tgatgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccacacctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009087
VERSION     KHUT01009087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           89 g           48 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggta ggtggttcgg
       61 taagtctgtt gtgaaagctc ctggcttaac cgggagaggc caatggaaac tgccggactt
      121 gaatcgtgga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaagca ggtgactgga cacgtattga cactgagcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009088
VERSION     KHUT01009088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           85 g           55 t
ORIGIN      
        1 tacgaggggt gctagcgtta ttcggaatta ctgggcgtaa agggtgcgta gacggcgtgc
       61 taagtctgtt gttaaatcct ccggcctaac cggagaatcg cgatggaaac tagtatgcta
      121 gaggatgaga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacatcaat ggcgaaggca gcttcttggc tcatttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009089             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009089
VERSION     KHUT01009089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ctcggaatca ttgggcttaa agcgcacgta ggcggcggcg
       61 taagtcagag gtgaaatccc atcggctcaa ccgtggaact gcctctgata ctgcgttgct
      121 cgagggagaa cggggcattc ggaacttccg gtggagcggt gaaatgtgtt gatatcggaa
      181 ggaacgccgg tggcgaaagc ggaatgctgg gtctcttctg acgctgaggt gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01009090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009090
VERSION     KHUT01009090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c          100 g           46 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtga ctgggtgtaa agggtccgta ggcgggagga
       61 caagtctgac gtgaaagcgc ccggctcaac tgggcgagtg cgttggaaac tgtttttctt
      121 gaatcacgca gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccgactggg ggatgattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009091
VERSION     KHUT01009091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 tacggagggt gctagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgg
       61 caagtcggat gtgaaagccc tgggcttaac ctgggaattg cattcgagac tgcctggcta
      121 gagtttggta gaggaaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gctttctgga ccataactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009092
VERSION     KHUT01009092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           96 g           41 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ctgggcgtaa agggcgcgta ggcggccaag
       61 caagtccagg gtgaaatccc tcggcccaac cgaggaactg ccctcgaaac tgcttggctg
      121 gaggccggta gaggacagtg gaattcccag tgtagcggtg atatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctgtctggg ccggtactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009093
VERSION     KHUT01009093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ctcggaatta ctgggcataa agagcgcgta ggttgcgcag
       61 taagtcgata gtgaaatcct atggctcacc catagaatgg ctagcgatac tgctgtgctt
      121 gagggcagga ggggagagtg gaactccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacgccggt ggcgaaagcg actctctgga ctgcacctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009094
VERSION     KHUT01009094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           86 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggatttg
       61 taagttatgt gtgaaatctc tgggcttaac ctgggaactg cacataatac tgtaagtcta
      121 gagtagagta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctttctgga ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009095
VERSION     KHUT01009095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           83 g           58 t
ORIGIN      
        1 tacgaaggtg gctagcgttg ttcggattta ttgggcgtaa agggtccgca ggcggtttga
       61 taagtcaggt gtgaaatcca acagcttaac tgttgacacg catttgatac tgtcagacta
      121 gagtatgaga ggggtgagag gaatttccag tgtaggggtg aaatccgtag atattggaaa
      181 gaacaccggc cgcgaaggcg tttcactggc tcattactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009096
VERSION     KHUT01009096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgggcgca ggcggccatt
       61 caagtcaggg gtgaaatcct ggggcttaac cccagaattg cctttgaaac tgtatggcta
      121 gagtattgga ggggatagcg gaattcctaa tgtagtggtg aaatacgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga caattactga cgctgaggtc cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009097
VERSION     KHUT01009097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           96 g           41 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggcccgg
       61 aaagtcggat gtgaaacccc gaggctcaac ctcgggactg catccgaaac ttccgggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009098
VERSION     KHUT01009098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           43 t
ORIGIN      
        1 tacgaagggc ccgagcgttg cgcggaatca ctgggcttaa agcgtacgca ggcgggcgcg
       61 caggcgtttt gtgaaagccc tcggctcaac cgaggaactg cttgacgaac cgcgcgtctc
      121 gaggcaggta ggggctgtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggagaaatca ggcagctggg cctgttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009099
VERSION     KHUT01009099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcga
       61 taagtccgtt gtgaaagtcg agggctcaac cctcgaatgc cggcggatac tgtcggacta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009100
VERSION     KHUT01009100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           53 t
ORIGIN      
        1 gacgaacctt gcaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaatag cattcaaaac tactgtgcta
      121 gaggaataca gaggtaagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tattttctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009101             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009101
VERSION     KHUT01009101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           98 g           36 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggagggg
       61 aacgtcggtt gctgaaagcc cccggctcaa ccggggaagg ggcgccgaaa cgacccgtct
      121 ggaggcacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtgtactg acgctgagac gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009102             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009102
VERSION     KHUT01009102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttcg
       61 taagtcggat gtgaaaactc agggctcaac ccggagacgc catccgatac tgcgatgact
      121 ggagtctggt aggggagcac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccgg tggcgaaggc ggtgctctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009103
VERSION     KHUT01009103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          100 g           44 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtggtc
       61 caagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tgggcggctg
      121 gagtgccgga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggtgtagacg gcttgctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009104
VERSION     KHUT01009104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           94 g           43 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggttggg
       61 taagtctgat gtgaaatccc cgggcccaac ccgggaacgg cattagaaac tgcccggctg
      121 gagggttgga ggggggactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacgccagt ggcgaaggcg agtccctgga caacacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009105
VERSION     KHUT01009105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           90 g           44 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggcggcc
       61 taagtacctt gtgaaatcct gcggctcaac cgcagaactg cttggtatac tgggtcgctt
      121 gagccatcca ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg gatgcgctga cactgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009106
VERSION     KHUT01009106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           89 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggttgtattg
       61 taagttagtt gtgaaagccc tgggcttaac ctaggaattg caactaagac tgtgatacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009107
VERSION     KHUT01009107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           31 c           69 g           66 t
ORIGIN      
        1 tacaggggat gcgagtgtta tccggaatta ttgggcgtaa agcgtctgca ggtggatttt
       61 taagtctttt gttaaatatt taagcttaac ttaaaaattg caaaggaaac taaaattcta
      121 gagtttggta gaggtaaagg gaatttccag tggaatggtg aaatatatag atattggaaa
      181 gaacaccaaa ggcgaaggca ttttactggg ccataactga cactgagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009108
VERSION     KHUT01009108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgtgta ggaggtggcg
       61 taagtcagct gtgaaatccc agggcttaac cctggaactg cagccgatac tgcgttgcta
      121 gaggatcgga gggggatgcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcatcctgga cgattcctga ctctgaaaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009109
VERSION     KHUT01009109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           58 c           91 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcgggccgt
       61 aaagtcgagg gtgaaatccc ccagctcaac tggggaactg cctccgatac tgacggcctc
      121 gagtatgata ggggcgtgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcacgctgga tcatcactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009110
VERSION     KHUT01009110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           83 g           55 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggtttat
       61 taagtttatt gttaaagact tcggcttaac tgaagaaaag caacgaatac tggtagacta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggct gcttcttggc tcactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009111
VERSION     KHUT01009111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtagga
       61 taagtttggg gtgaaagctc cgggctcaac ccggaaatgg cctcgaagac tattctgcta
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctcttga cgatttctga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009112
VERSION     KHUT01009112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaattg ctgggcatac tggatgtctt
      121 gagtggccta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg ggccaactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009113
VERSION     KHUT01009113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcgaaatta ctgggcgtaa agcgtgcgca ggcggcttgg
       61 caagaccgat gtgaaatccc cgggcttaac ctgggaatgg catgggtgac tgccaagctt
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009114
VERSION     KHUT01009114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 caagtcagtg gtaaaatctc ccggctcaac tgggagaagc ccgttgatac tggcaggctt
      121 gagggaggca gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg accttctggg ccttgcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01009115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009115
VERSION     KHUT01009115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtcttc
       61 taagtctggt gtgaaagccc agggcttaac cctggaagtg cactggatac tgggagacta
      121 gagggcgaga ggggaaagcg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gctttctggc tcgtttctga cgctcagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009116
VERSION     KHUT01009116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcgggtcgt
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctttgatac tggcgacctg
      121 gagtccggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009117
VERSION     KHUT01009117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           92 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacgac
       61 caagtcaagg gtgaaatctt tcggctcaac cggaaaacag cccctgatac tggccgtctt
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg gcgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009118
VERSION     KHUT01009118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 tacagagggc tcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggacgcg
       61 caggcacttt gtgaaatccc ccggctcaac cggggaattg cttggtgaac cgcgcgcctt
      121 gaggcaagta ggggctgtcg gaacgctagg tggagtggtg aaatgcgttg atatctagcg
      181 gaacgccgaa ggagaaatca ggcagctggg cttgtcctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009119
VERSION     KHUT01009119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           89 g           52 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcggtcgtt
       61 caagtcgggg gtgaaatctt gcagcttaac tgcaaaagtg ccttcgatac tgatcgactc
      121 gagcgagata ggggcaggtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009120
VERSION     KHUT01009120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 ttagtcgggg gtgaaatcgt tcggcccaac cggacaaggg cccccgatac tggcagtctc
      121 gagggaggta ggggttagtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gctaactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009121             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009121
VERSION     KHUT01009121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           84 g           51 t
ORIGIN      
        1 tacaggggga gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtctag
       61 aaagtctact gtcaaatacc aaggcttaac cttgggcagg cagtagagta cttttagact
      121 tgagtgcggt agaggtagag ggaatcccta gcgtaacagt gaaatgtgta gattttaggg
      181 agaacaccag cggcgaaggc gctctactgg gccgagactg acactgagag acgaaagcta
      241 agggagcgaa aagg
//
LOCUS       KHUT01009122             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009122
VERSION     KHUT01009122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           42 c           95 g           55 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggttatg
       61 taagtctggt gtgaaatctt catcgctcaa cgggaagagg tcactggaaa ctgcgtaact
      121 cgagtgtggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatcgg tggcgaaggc ggcttgctgg gccattactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009123             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009123
VERSION     KHUT01009123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggagtta ctgggcttaa agagttcgta ggcggcgctg
       61 tcagtgagag gtgaaatccc aacggctcaa ccgtggaatt gcctttcata ctgcagcgct
      121 tgagggaagt aggggcgagt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccgg tggcgaaagc gactcgctgg gtttctcctg acgctgagga acgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009124
VERSION     KHUT01009124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttat
       61 taagtcagct gtgaaagccc tgggcttaac ctgggaattg cagttgatac tggtaagcta
      121 gagtgtggta gagggtggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gccacctgga ccaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009125
VERSION     KHUT01009125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggacttg
       61 caggcatgtt gtgaaatccc atggctcaac catggaattg cttcgtgaac cgcaagtctt
      121 gaggctggta gaggcggtcg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgccaaa ggtgaagaca ggccgctggg ccagacctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009126
VERSION     KHUT01009126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttgca
       61 tgggtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gatgtaactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009127
VERSION     KHUT01009127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgaacgta ggcggccaag
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgaaac tgcatggcta
      121 gaatcctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caggtattga cgctgaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009128
VERSION     KHUT01009128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           86 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggtatca ctgggcttaa agcgtgcgta ggcggcctag
       61 taggtgggat gtgaaagccc tcggctcaac cgaggaattg cgttccaaac cactaggctc
      121 gaggatggca ggggtaatgg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg cattactggg ccatttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009129
VERSION     KHUT01009129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgca ggcggtctgt
       61 taagtctgat gtgaaagccc ggggctcaac cccggaatgt cgttggatac tggcagacta
      121 gagttcggaa gaggagagcg gaattcctag tgtagcggtg aaatgcgcag atattaggag
      181 gaacacctac ggcgaaggca gctctctggt acgtaactga cgctgagacg cgaaagtgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009130
VERSION     KHUT01009130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           92 g           44 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggtggaggtt
       61 taagtcactt gtgaaagctc ccggctaaac tgggagaggt caggtgagac tggacatcta
      121 gaggatgtca gagggatgca gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcatcctggg acatacctga cactgagacg cgaaagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009131
VERSION     KHUT01009131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           78 g           61 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggcgtc
       61 taagtcagtg gtgaaatccc gcagctcaac tgtggaattg ccattgatac tggtcgcctt
      121 gaatcaatgt gaagtggcta gaatatgtag tgtagcggtg aaatgcttag atattacata
      181 gaataccgat tgcgaaggca ggtcactaac attgcattga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009132
VERSION     KHUT01009132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcggatagg
       61 caagtctgac gtgaaagcgc tcggctcaac tgagcgatgc cgttagaaac tgcttatctt
      121 gaatcaggca gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacgccaaa ggcgaaggca gccggctagg ccatgattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009133
VERSION     KHUT01009133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           83 g           55 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttttg
       61 ttagtcttct gttaaagctc ccggcttaac cgggaaagtg cagaggaaac ggcaagacta
      121 gaggatgtga gaggtaagcg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gcttactggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01009134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009134
VERSION     KHUT01009134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           87 g           60 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgtgaggcta
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactagg ctgttactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009135
VERSION     KHUT01009135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggtgtaa agggtgcgca ggtggtccgc
       61 caagtctgat gtgaaatccc tgggctcaac tcaggaatgg cattggaaac tggcgggcta
      121 gagtaccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gcttcctggc cgtgtactga cactcatgca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009136
VERSION     KHUT01009136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           88 g           47 t
ORIGIN      
        1 gacgaaccgc ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcccag
       61 caagtcaggt gtgaaatccc acggctcaac cgtggaactg cgcttgaaac tgctgggctt
      121 gagggagata gaggtaagcg gaactgatgg tggagcggtg aaatgcattg atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009137
VERSION     KHUT01009137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          105 g           38 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcacgta ggtggcgcgt
       61 tgcgtcggcg ctgaaagcgc cgggcttaac ccggcgaggt gcgccgatac gagcgtgctc
      121 gaggcaggga gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgccagt gacgaaggtg gccacctggc cctcgcctga cactgaggtg cgatagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009138
VERSION     KHUT01009138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgca ggcggtttga
       61 caagtcatgc gtgaaagctc ctggctaaac tgggagaggt cggatgagac tgtcaggcta
      121 gagggtgtta gaggcgtgtg gaattccgcg tgtagtggtg aaatgcgtag agatgcggag
      181 gaacaccagt ggcgaaggcg gcacgctggg acatcactga cgctgagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009139
VERSION     KHUT01009139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           85 g           49 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatca ttgggcttaa agggtgcgta ggcggtactg
       61 taagtcgggt gtgaaatacc tcagctcaac tgaggaacag cgcccgatac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009140
VERSION     KHUT01009140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggcgtcg
       61 caagtcaggc gtgaaatccc cgggctcaac ctgggaattg cgcttgaaac tacgatgctg
      121 gagtacggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccttctggg ccgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009141
VERSION     KHUT01009141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           84 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggactgt
       61 taagtcagtg gtgaaagccc cgggctcaac ctgggaattg ccattgatac tggcggtctt
      121 gagtacagta gaggtaggcg gaatgcaaca tgtagcggtg aaatgcttag atatgttgca
      181 gaacaccgat tgcgaaggca gcttgctatg ctgttactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009142
VERSION     KHUT01009142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          104 g           40 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtccgta ggcggcctgc
       61 tgcgtcgctg ctgaaagcgc cccgcttaac ggggcgaggc gtggcgatac gagcaggctt
      121 gaggaaggca gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgcctgt ggcgcaagcg gccacctggg ccttatctga cgctgaggga cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01009143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009143
VERSION     KHUT01009143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggtgtaa agggagcgca ggcgggatag
       61 caagtcagct gtgaaaacta tgggctcaac ccataaactg cagttgaaac tgttattctt
      121 gagtggagta gaggcaagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcttgctggg ctctaactga cgctgaggct cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009144             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009144
VERSION     KHUT01009144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           59 c          101 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggcctgg
       61 ccaagtcgtg cgtgaaatct cccggctcaa ctgggagcgg tcgtgcgata ctggtcggct
      121 cgaggccggt aggggggagt ggaattccgg gtgtagtggt ggaatgcgta gatatccgga
      181 ggaacaccag tggcgaaggc ggctccctgg accggtcctg acgctcagga gcgaaagcgt
      241 gggtagcgat ccgg
//
LOCUS       KHUT01009145             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009145
VERSION     KHUT01009145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           62 c           85 g           43 t
ORIGIN      
        1 caccagcgcc acgagtggct atcacgatta ttgggcctaa agcgtccgta gtcggttctg
       61 tgcatcttct gtgaaatcgt tgcgcttaac gtaacggcac gcgggagaca cggcagaact
      121 tgagagcggg aggcgtgtag ggtactccgg ggggaccggt aaaatggtat aatccccgga
      181 agaccacctg tggcgaaggc gctacacgag aacgcatctg acgatgaggg acgaaagcca
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01009146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009146
VERSION     KHUT01009146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttaga
       61 tgtgagtgat gtgaaagccc tgggcttaac ctaggaagcg catcgcagac tatctagctg
      121 gagtagatga gagggtgatg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gtcacctggc atcatactga cgctgaagcg cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01009147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009147
VERSION     KHUT01009147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           81 g           52 t
ORIGIN      
        1 tacgtagggg ccaagcgtta tccggaatta ctgggcgtaa agcgtacgta ggcggttgac
       61 taagttgggt gtgaaattct acggctcaac cgtagaacta tgcccaaaac tagttaacta
      121 gagcatagta gaggcaagtg gaacttgcgg tgtaggagtg acatccgtag atatcgcaaa
      181 gaacaccaat ggcgaaggca gcttgctggg ctattgctga cgctcaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009148
VERSION     KHUT01009148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           91 g           43 t
ORIGIN      
        1 tacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc accgctcaac ggtggaacgg ctgggcatac tggcggtctt
      121 gagccaccta ggggcagctg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaac ggtgaaaaca ggctgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009149
VERSION     KHUT01009149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          105 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggagact
       61 taagtggtca gtgaaagccc ccggctcaac cggggagagt ctggccagac tgggcctctg
      121 gaggacagta gaggagagtg gaattgctgg tggagcggtg gaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gctctctgga cttgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009150
VERSION     KHUT01009150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtgcgca ggcggcctat
       61 taagtcagtg gtgaaatcct tccgcttaac ggaagaactg ccattgatac tgattggcta
      121 gaatatagtt gaagtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat agcgaaggca gcttactaag ctattattga cgctcaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01009151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009151
VERSION     KHUT01009151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 gacggggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggcgta ggtggccatg
       61 taagttagga gtgaaagccc tgggctcaac ccaggaagtg ctcttaatac tgcgtggctt
      121 gaatgtggga gagggtagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctacctggc ccaacattga cactgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009152
VERSION     KHUT01009152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatca ctgggcgtaa agggagcgca ggcggtcgga
       61 ttagtccaaa gtgaaatccc ggggctcaac ctcggaagtg ctttggatac tgcccgactt
      121 gagtacgcga gaggtgagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactggc gcgatactga cgctgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009153
VERSION     KHUT01009153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggtccct
       61 taagttcggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgagggactc
      121 gagtgcggga ggggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgcagacg gctctctgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009154
VERSION     KHUT01009154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggctctt
       61 taagtcagat gtgaaatcct gcggctcaac cgcagaactg catttgatac tggagggctt
      121 gagtgtggga gaggaaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gctttctggc ccaccactga cgctgagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009155
VERSION     KHUT01009155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           65 c          113 g           27 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtcctc
       61 aacggcggac gtgaaagccc ccggctcaac cggggcgggt cgtccgcgac ggggggactg
      121 gaggctgaca gaggggggtg gaatgccggg tggagcggtg aaatgcgtag agacccggcg
      181 gaacaccggt ggcgaaggcg gccccctggg gcagacctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009156
VERSION     KHUT01009156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctttt
       61 caagtcggac gtgaaatccc ttggcttaac taaggaactg cgtccgatac tgctaagcta
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009157
VERSION     KHUT01009157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggcccct
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccggatac tgaagggcta
      121 gagtgcggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtaaacg gctttctgga ccgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009158
VERSION     KHUT01009158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacagaggcc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggaa
       61 taagtcggat gtgaaatccc ggagcttaac tccggaactg cattcgatac tgtttcgcta
      121 gaggactgga gaggagatcg gaattcacgg tgtagcagtg aaatgcatag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009159             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009159
VERSION     KHUT01009159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggattgt
       61 taagtgcgat gcgaaatcct gtggctcaac catatggact gtatcgcata ctggcaatct
      121 tgaggacatg agaggcaagt ggaattactg gtgtagcagt gaaatgcgta gagatcagta
      181 ggaacaccaa tggcgaaggc agcttgctgg catgtatctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009160             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009160
VERSION     KHUT01009160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           77 g           56 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggaatta ctgggcgtaa agcgtgcgca gactgtttag
       61 taggtcttat gcgaaatccg gtggctcaac cacctggact gtatgagaaa cccctagact
      121 tgaggtaggt agaggtacat ggaatttctg gtgtaggagt gacatccgta gatatcagaa
      181 ggaacaccaa tggcgaaggc agtgtactgg gccttacctg acgttcaggc acgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01009161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009161
VERSION     KHUT01009161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           90 g           51 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaatgg cccctgatac tgattgtctt
      121 gagggagata ggggccatgg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg catggctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009162             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009162
VERSION     KHUT01009162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           48 t
ORIGIN      
        1 tacgtagggg tctagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggttcga
       61 ttagtcgggt gtgaaatctc cacgctcaac gtggaggggc cactcgatac tgtcgtgact
      121 cgagtccggt aggggagttc ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggaactctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009163
VERSION     KHUT01009163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           98 g           48 t
ORIGIN      
        1 tacggaggcc ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcgtgg
       61 taagtctggt gtgaaaggcg tgggctcaac ccgcggactg cattggatac tgccatgcta
      121 gaggactgga gaggagaatg gaattcacgg tggagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaggcg attctctgga cagtaactga cgctgaggca cgaaggctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01009164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009164
VERSION     KHUT01009164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           97 g           44 t
ORIGIN      
        1 cacgtaggag cctagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggctggg
       61 taagttggct gtgaaatctc ccggctcaac tgggagggga cggtcaagac tgcccggctg
      121 gaggtaggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga cctgtcctga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009165
VERSION     KHUT01009165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          103 g           39 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggtggggtgc
       61 caagcggggt gtgaaagcgc ctggcttaac tgggcgagtg cgctccgaac tggcaaccta
      121 gagggcaggc gacggaggtg gaatacccgg tggagtggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gccttcgagc ctgcagctga cactgaagcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009166             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009166
VERSION     KHUT01009166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           45 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggatttg
       61 caagcctgag gtgaaaggtt ggggctcaac cccatacagt gccttgggga ctgcaagtct
      121 ggaggccggc agaggagagt ggaattcctg gtgtagcggt ggaatgcgta gagatcagga
      181 agaacaccca tggcgaaggc agctctctgg gccggtactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01009167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009167
VERSION     KHUT01009167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           90 g           44 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtcgggt gtgaaatccc tcggctcaac cgaggaatgg cgctcgaaac taccgtgctc
      121 gaggaaggca gaggtgagcg gaactcaggg tggagcggtg aaatgcgttg atatcctgag
      181 gaacaccggt ggcgaaagcg gctcactggg ccttttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009168
VERSION     KHUT01009168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggctcgg
       61 ttagtggaat gtgaaatgcc ctggctcaac cagggaactg cattccatac tgccgagctt
      121 gagtatagga gaggatgggg gaattcccgg tgtagcggtg gaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggc ctaatactga cgctcaagcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009169
VERSION     KHUT01009169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtctgg
       61 tcagtcaggt gtgaaagccc tcggctcaac cgaggaatcg cacttgatac tgccagactc
      121 gagagaggta ggggtaagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactgga cctcttctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009170
VERSION     KHUT01009170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttgt
       61 taagtcagct gtgaaagccc cgggctcaac ctgggaattg ctgttgatac tggcaagcta
      121 gagtgtggta gagggtagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gctacctgga ccaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009171
VERSION     KHUT01009171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           97 g           40 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcttat
       61 caagttgggc gtgaaagccc ggggctcaac cccggaaatg cgcccaagac tggtaggcta
      121 gagaccgaga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccacctggc tcggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009172
VERSION     KHUT01009172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggttcg
       61 taagttgatt gtgaaatccc cgagctcaac ttgggaattg cagtcaaaac tgcgagtctt
      121 gaatacctga gagggtggga gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaataccgga ggcgaaggcg cctacctggc agagtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009173
VERSION     KHUT01009173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           72 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtagg
       61 acagtcagtg gtgaaatctt caagcttaac ttggaaactg ccattgatac tatctatctt
      121 gaatactttg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca cagtcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009174
VERSION     KHUT01009174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           40 t
ORIGIN      
        1 tacggagggg gctagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggcggcctgt
       61 taagttggat gtgaaagccc ggggctcaac cccggatgtg catccgagac tggcaggctg
      121 gagtccggga gagggcggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccgcctggc ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009175
VERSION     KHUT01009175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatta ctgggtgtaa agggtgcgta ggcgggcgtg
       61 taagtcaggg gtgaaatcca ccggcctaac cggtgaactg cccttgatac tgcacgtctt
      121 gagtctcgga gaggctgtcg gaattcgtgg tgtagcggtg aaatgcgtaa atatcacgag
      181 gaacaccaga tgcgtaggcg gacagctgga cgagtactga cgctcaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009176
VERSION     KHUT01009176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggcttct
       61 taagtctgat gtgaaatccc agggctcaac cctggaactg catcggatac tgaaaagcta
      121 gagggtgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gccatctggc tcacctctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009177
VERSION     KHUT01009177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacgagaggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggatgcg
       61 caagcgtctt gtgaaatccc tcgtctcaac cgaggaattg cggggcgaac tgcgtgtctt
      121 gaggcaggta ggggtcgtcg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgcctat ggagaaatca gacgactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009178
VERSION     KHUT01009178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattag
       61 taagtctgtg gtgaaatctc cgagcttaac tcggaaactg ccatggatac tattagtctt
      121 gaatctcctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg agagtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009179
VERSION     KHUT01009179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtgtgg
       61 taagttatct gtgaaatccc cgggcttaac ctgggagggt cagataatac tgctgcacta
      121 gagtatgaga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctggc ttaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009180
VERSION     KHUT01009180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgta ggcggtctct
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggagactt
      121 gagtacagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgcag agatatggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009181
VERSION     KHUT01009181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 cacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgatt
       61 caagtcaggt gtgaaaggct tcagctcaac tggggcactg cgcttgaaac tggattgctg
      121 gagtatctca ggggtgtgcg gaactgccag tggagcggtg aaatgtgttg atattggcag
      181 gaacaccgga ggcgaaggcg gcacactggg ggatcactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009182
VERSION     KHUT01009182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgt
       61 taagtcaggc gtgaaatccc tgggctcaac ctgggaactg cgtttgatac tggcagactc
      121 gagtctggta gagggtagtg gaattccgca tgtagcggtg aaatgcgtag atatgcggag
      181 gaacatcagt ggcgaaggcg gctacctgga ccaagactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009183
VERSION     KHUT01009183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           87 g           57 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggcggtctgg
       61 taagtcagct gtgaaaacgc ggggctcaac tccgcgcctg cagttgatac tgtcggactt
      121 gagtgcacgc agggatgccg gaattcatgg tgtagcggtg aaatgcttag atatcatgaa
      181 gaactccgat cgcgaaggca ggtgtccgga gtgctactga cgctgaggct cgaaagtgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01009184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009184
VERSION     KHUT01009184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           91 g           54 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggacgtt
       61 taagttagag gtgaaatcct gcggctcaac cgcagaactg cctttaatac tggatgtctt
      121 gaggtcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009185
VERSION     KHUT01009185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacggagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgca ggcggaacac
       61 atagtcaggc gtgaaagccc cgggcttaac ctgggaactg cgtttgatac ttgtgatctg
      121 gagtctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccacctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009186
VERSION     KHUT01009186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          108 g           38 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgta ggcggctact
       61 cgcgtcgcac gtgaaagccc ccggcttaac tggggagggt cgtgcgaaac ggagtggctg
      121 gaggcaggga gaggctggtg gaattcccgg agtagtggtg aaatgcgtag ataccgggag
      181 gaacaccggt ggcgaaggcg gccagctggc cctgacctga cgctgaggtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009187             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009187
VERSION     KHUT01009187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          101 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 caagtcggtg gtgaaagccc gtggctcaac tacgggtcgg ccaccgatac tgctatggct
      121 cgagtttggt agaggcagat ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggtctgctgg gccaacactg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009188             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009188
VERSION     KHUT01009188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtcgg
       61 cacgtcggtc gctgaaatcc cccggctcaa ccggggaagg ggcgccgata cggccggtct
      121 tgagcaacgt agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagctcctgg acgttttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01009189             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009189
VERSION     KHUT01009189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           80 g           56 t
ORIGIN      
        1 gacgtaggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcataa
       61 taagttattg gtggtaaatc gaggctcaac ctcgtggaag ctggtaatac tgttatgctc
      121 gagtgcagaa gaggttaacg gaactccctg tggagcggta aaatgtgaag atatagggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgctac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009190             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009190
VERSION     KHUT01009190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           86 a           31 c           74 g           65 t
ORIGIN      
        1 tacgagaggg gcaagcgtta ttcggaatga ttgggcgtaa agagcacgta ggcggtttat
       61 aaaatctaga aaagtaatct caaggcttaa ctttgaatag atttctgtat gattaataaa
      121 ctggagtaag taaaaggaga atggaattcc tggagtagag gtaaaatttg ttgatttcag
      181 gaggaacacc gaaagcgaag gcaattctct gatgctttac tgacgttgag gtgcgaaagc
      241 gtggggatca aaaggg
//
LOCUS       KHUT01009191             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009191
VERSION     KHUT01009191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           81 g           54 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gacggtctag
       61 taagttagca gcgggaaatc gaggcccaac ctcgtggaag ctgttagtac tgctatactc
      121 gagtgcagga gaggttaacg gaactcaatg tggagcggta aaatgtgtag atatattgaa
      181 gaacaccaat aacggcgaag gcagttaact attctgctac tgacgttgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01009192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009192
VERSION     KHUT01009192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           45 c           79 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agagcacgta ggcggagtgg
       61 aaagttagat gtgaaatccc gaggctcaac ctcggatacg catctaaaac tcccattcta
      121 gaggatagac ggagaaaagg gaattccacg tgtagcggta aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg cttttctagt ttatacctga cgctgaggag cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009193
VERSION     KHUT01009193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           85 g           54 t
ORIGIN      
        1 gacggggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtgtgca ggtggctctg
       61 taagtcagat gtgaaagccc cgggcttaac tcgggaagtg catttgaaac tacagaactt
      121 gaatatggaa gagggtcgtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgacctggt ccaatattga cactcataca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009194             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009194
VERSION     KHUT01009194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           39 c           81 g           57 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtttga
       61 aaagttagtg gtggtaaatc gaggcccaac ctcgtggaaa ctattaatac tgtcagacta
      121 gagagcagga gaggttaacg gaactccacg tggagcggta aaatgtgacg atatgtggat
      181 gaacaccaat aatggcgaag gcagttaact attctgtttc tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01009195             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009195
VERSION     KHUT01009195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           82 g           57 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtctag
       61 taagttagca gtgggaaatc gaggctcaac ctcgtggaag ctggtaatac tgttaggctt
      121 gagtacagga gaggttaacg gaactccatg tggagcggta aaatgcatag atatatggat
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009196             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009196
VERSION     KHUT01009196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           37 c           84 g           57 t
ORIGIN      
        1 tacggggggg gcaagcgtta ttcaaattga ctgggcgtaa agagcacgta ggcggttttt
       61 taagttttga gtgggaaaaa gccaaggctt aaccatggtt tttccaagta gactattaaa
      121 ctagaggaag ggagaggaga gtggaattcc tggaggaaag gttaaatttg tagatctcag
      181 gaggaagatc gaaagcaaag gcaactctct ggccctttac tgacgctgag gtgcgaaagc
      241 atggggagcg aagagg
//
LOCUS       KHUT01009197             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009197
VERSION     KHUT01009197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          102 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggccatt
       61 ccgtaagtcg gtcgtgaaat ccccgggctc aacccggggg ggtcggtcga tactgcgggg
      121 ctttgaaggc ggtaggggga gatggaattc ccggtgtagt ggtgaaatgc gtagatatcg
      181 ggaggaacac cgatggcgaa agcgatctcc tgggccgttc ttgacgctga ggcgcgaaag
      241 cgtggggagc gaactga
//
LOCUS       KHUT01009198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009198
VERSION     KHUT01009198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           82 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagtgta ggcgggtttg
       61 taagtcagtg gtgaaagccc cgggctcaac ttgggaattg ccattgatac tgctagcctt
      121 gagtaaggtt gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcactaag cctttactga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009199
VERSION     KHUT01009199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           64 c           99 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggccgg
       61 tcagtcaggg gtgaaatccc gcggcccaac cgcggaaggg cctctgatac tgccggcctc
      121 gagggggata ggggcgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tccccactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009200
VERSION     KHUT01009200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           45 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggttggc
       61 aaagtcggat gtgaaaatcc ggggctcaac cccggacctg catccgatac ttgtcgactc
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009201
VERSION     KHUT01009201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           90 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgtgtgta ggcggtccga
       61 aaagtggggt gtgaaagccc tgggctcaac ccaggaactg cgccccaaac tgccggactt
      121 gagtgtcgga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009202
VERSION     KHUT01009202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcgg
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tgccagactt
      121 gaatatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009203
VERSION     KHUT01009203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           85 g           54 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggttttg
       61 taagtaagaa gtgaaagcct ggggcttaac ctcagaattg ctttttagac tgcagaacta
      121 gaatcctgga gaggttagtg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccagt ggcgaaggcg actaactgga caggcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009204
VERSION     KHUT01009204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           93 g           51 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggttcgg
       61 caagtacttt gtgaaatacc tgggctcaac ctgggaattg cttggtatac tgccggactt
      121 gaggcggata ggggtgcttg gaactctagg tggagcggtg aaatgcgtag atatctaggg
      181 gaacgccaga ggcgaaagcg gagcactggg tcttgcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01009205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009205
VERSION     KHUT01009205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctgct
       61 caagtcgggg gtgaaatctt tccgctcaac gggaaaatag cctccgatac tgggtagctc
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009206
VERSION     KHUT01009206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtcttt
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg cattcgaaac tgggaggcta
      121 gagtacagaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009207
VERSION     KHUT01009207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggccgaa
       61 taagtcagtg gtgaaagccc gcagcttaac tgcggaactg ccattgaaac tgctcggctt
      121 gagtactatt gaagtgggtg gaatgcatca tgtagcggtg aaatgcttag atatgatgca
      181 gaaccccgat tgcgaaggca gctcactaaa tagtaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009208
VERSION     KHUT01009208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgttcgta ggcggcttgc
       61 caagtcagag gtgaaatccc acgactcaat cgtggaactg cctttgagac tggttcgctt
      121 gaacacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgttgttga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009209
VERSION     KHUT01009209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           86 g           56 t
ORIGIN      
        1 tacgaaggtg gctagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtttga
       61 aaagtcaggt gtgaaatccc acagcttaac tgtggacgcg catttgaaac tttcagacta
      121 gagtgtgaga ggggtgaagg gaatttccag tgtaggggtg aaatccgtag atattggaaa
      181 gaagaccggt cgcgaaggcg cttcactggc tcactactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009210
VERSION     KHUT01009210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           87 g           44 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcagcttaac tgaggaatgg cgcccaaaac taccgtgctc
      121 gaggaagaca gaggtgagcg gaactcaggg tggagcggtg aaatgcgttg atatcctgag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttatctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009211
VERSION     KHUT01009211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           94 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccacg
       61 caagtcgggt gtgaaatccc ggggctcaac cccggaagtg cactcgaaac tgcatggctg
      121 gagtctcgga gaggaaggcg gaattctagg tgtagaggtg aaattcgtag atatctagag
      181 gaacaccggt ggcgaaggcg gccttctgga cgagcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009212
VERSION     KHUT01009212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           97 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtcggc
       61 aaagtcgggt gtgaaaaccg ggggctcaac ccccggcctg catccgatac tcgtcgacta
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009213
VERSION     KHUT01009213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggagcgta ggcggtagcg
       61 taagtcggat gtgaaatctt ggggctcaac ctcaaaactg cactcgatac tgcgttgcta
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagc ggcgaaggcg ggtctctgga cagttcctga cgctgatgct cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009214
VERSION     KHUT01009214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 gacggaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaagtg cgcccaaaac tactgtgctg
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009215
VERSION     KHUT01009215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcaacg
       61 taagtcagag gtgaaagctc acggctcaac tgtggaattg cctttgatac tgcgtagctt
      121 gaatgagtgc gaggcaggcg gaatgtggca tgtagcggtg aaatgcttag agatgccata
      181 gaacaccgat tgcgaaggca gcctgccaga actctattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009216
VERSION     KHUT01009216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtccgtg gtgaaagcct ccagcttaac tggagaagtg ccgcggatac tggtgacctc
      121 gagggtggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccacttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009217             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009217
VERSION     KHUT01009217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           86 a           36 c           76 g           58 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtttta
       61 taagttatta gtagaaaact aaggctcaac cttaggaaag ctaataatac tatatgacta
      121 gagtgcagga gaggttagcg gaattccctg tggagtagtg aaatgcgtag atatagggaa
      181 gaacaccaat agaggcgaaa gcagctaact agtctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009218
VERSION     KHUT01009218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           87 g           52 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgct
       61 taagtacctt gtgaaatccc acggcttaac cgtggaattg cttggtatac tgtgcgtctt
      121 gagccactca ggggctaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca ggtagctggg ggtgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009219
VERSION     KHUT01009219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           97 g           41 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccgtg
       61 taagtcgggt gtgaaacccc ggggctcaac cccgggcctg catccgatac tgcacggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009220
VERSION     KHUT01009220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           45 c           77 g           55 t
ORIGIN      
        1 cacgtaggtc gcgagcgtta tccggaatta ttgggcgtaa agcgttcgta ggtggccggg
       61 caagtcttgt gtgaaagcca acagcttaac tgttgaactg cacaagatac tatccggcta
      121 gaatttagga gaggaaactg gaattccatg tgtagcggtg gaatgcgtag atatatggag
      181 gaacaccaat agcgaaggca ggtttctgga ctaacattga cactaaggaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009221
VERSION     KHUT01009221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           76 g           59 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattat
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaactg ccattgatac tgataatctt
      121 gagtatgatt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccaatactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009222
VERSION     KHUT01009222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           39 c           90 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgaa
       61 taagttatgt gtgaaattcc taggcttaac ctgggcattg cagataagac tgtttgacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009223
VERSION     KHUT01009223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggttcgt
       61 taagtcgtct gtgaaagccc atggctcaac catggaagtg cagccgatac tggcaaactt
      121 gagtcccgga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctgga cgggtactga cgctgagacg cgaaagcgcg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009224
VERSION     KHUT01009224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggtccgg
       61 taagtccgct gtgaaagtca ggggctcaac ccctgaatgc cggtggatac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009225
VERSION     KHUT01009225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcgggctga
       61 ttagtccggg gtgaaatctc acggctcaac cgtgagcggt ccccggatac tgtcagcctt
      121 gagggatcta gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gctctctggg gatcacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009226
VERSION     KHUT01009226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           56 c          104 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggcgcgg
       61 caagtgatcg gtgaaatcct ccggctcaac tggagggggt ccggtcggac tgccgtgctc
      121 gaggtcgcga gggggaagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc gcgaatctga cgctgaggtg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01009227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009227
VERSION     KHUT01009227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 gacgtaggtg gcgagcgttg ttcggaatta ctgggcataa agcgcgcgta ggcggccatg
       61 tcagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgtccgatac tgcatggctt
      121 gaggtccgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cggaatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009228             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009228
VERSION     KHUT01009228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           41 c           80 g           58 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcatga
       61 taagttatca gcggtaaatc gaggctcaac ctcgtggaaa ctggtaatac tgttatgctt
      121 gagtacagga gaggttaatg gaattccctg tggagcggta aaatgcgtta gatataggga
      181 ggaacaccaa taacggcgaa ggcaattaac tatcctgtta ctgacattga ggcacgaaag
      241 cgtggggagc aaaacgg
//
LOCUS       KHUT01009229             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009229
VERSION     KHUT01009229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           39 c           81 g           59 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtatga
       61 taagttattg gtggtaaatc ggggctcaac ctcgtggaag ctagtaatac tgttatactc
      121 gagtacagga gaggttaatg gaattccctg tggagcggta aaatgcgtta gatataggga
      181 ggaacaccaa taacggcgaa ggcaattaac tatcctgcta ctgacattga ggcacgaaag
      241 cgtggggagc aaaaagg
//
LOCUS       KHUT01009230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009230
VERSION     KHUT01009230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           83 g           50 t
ORIGIN      
        1 tacgaatggg gcgagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggcggtcgtg
       61 aaagttagaa gtgaaagccc agggctcaac cctggaattg cttttaaaac tccacgacta
      121 gaatactgga gaggttggcg gaattccgag tgtagcagtg aaatgcgtag atattcggag
      181 gaacaccgat ggcgtaggca gccaactgga cagttattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009231
VERSION     KHUT01009231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           88 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggattta ctgggcgtaa agcgtacgca gacggtttgg
       61 taggtcggat gtgaaagccc cgggctcaac ccgggaattg catccgaaac cacctcactc
      121 gagtgtggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gcttcctggc ccattactga cgttcaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009232
VERSION     KHUT01009232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           83 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggccgcgcgg
       61 gcagtcaaat gtgtaatcca ggggctcaac ccctgaactg cgattgatac ttccgcgcta
      121 gaactttgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacacttag ggcgtaggca agactctgga caaatgttga cgctcaagca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009233
VERSION     KHUT01009233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggtccgg
       61 aaagtcgggt gtgaaaaccc ggggctcaac cccgggcctg cattcgatac tgccgggcta
      121 gaggcagaca gggggaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctggg tctgtcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009234
VERSION     KHUT01009234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 tacagggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggccaac
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tggatggctt
      121 gagtgtagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gagcaccggt ggcgaaggcg gcattctgga ctacaactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009235
VERSION     KHUT01009235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           95 g           53 t
ORIGIN      
        1 tatgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggttagg
       61 caagttcgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tgcttagctt
      121 gaggcaatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc gttgttctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009236
VERSION     KHUT01009236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtta ctgggcgtaa agggtccgca ggcgggaagg
       61 caagtctgac gtgaaatctt ctggctcaac taggagaggt cgttggaaac tgcctttcta
      121 gcatcccgca gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg cgcggatgga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009237
VERSION     KHUT01009237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           95 g           52 t
ORIGIN      
        1 tacgtgaggg gcgaacgttg ttcggtgtca ctgggcttaa agggtgcgta ggtggtttcg
       61 taagtaaggt gtgaaagccc tcggcttacc cggggaattg cgccttatac tgcgagactt
      121 gaggatttca ggggaatggg gaactccagg tgtagcggtg aaatgcgcag atatctgggg
      181 gaaggccggc ggcgaaggcg ccattctggg aaattcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009238
VERSION     KHUT01009238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtcggg
       61 tcagtctgtg ctgacagtgt ccggcttaac tggacaagcg gtagagatac tgcccgactt
      121 gagggcttca gagggatggg gaactccagg tggagtggtg aaatgcgtag atatctggaa
      181 gaacaccaat ggcgaaggca ccatcctggg aagcatctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009239
VERSION     KHUT01009239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           82 g           51 t
ORIGIN      
        1 tacgtagacc tcaagcgtta tccggattta ttgggcgtaa agagcatgta ggaggcctcg
       61 tgcgtctctg gttaaattcc accgcctaac ggtggacctg ccagagatac gacgaagcta
      121 gaggaggtta gaggtgcata gaacgcacgg tgtaggggtg aaatccgttg atatcgtgcg
      181 gaataccaaa ggcgaaggca gtgcactggg accttcctga ctctgagatg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01009240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009240
VERSION     KHUT01009240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggctcgg
       61 agagtcgggt gtgaaatccg ggggctcaac ccccgaactg catccgatac ttccgagctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009241
VERSION     KHUT01009241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           40 a           58 c          117 g           38 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggattca ctgggcgtaa agggcgcgta ggcggctggc
       61 tcagtcgggc ctgtaagtgc ggggctcaac cccgtgatgg gctcgggacg ggctggctgg
      121 ggcgcggtcg agggagacgg aatgccgggt gtagtggtga aatgcgtaga gatccggcgg
      181 aacaccggtg gtgaaggcgg tctcctgggc cgagggccga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009242
VERSION     KHUT01009242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgtgcga
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgtcggactc
      121 gagtctcgga gaggttggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaataccggc ggcgaaggcg gccaactgga cgaagactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009243
VERSION     KHUT01009243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           82 g           49 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcctga
       61 caagttgggt gtgaaatacc tcggcttaac cgaggaacgg cgctcaaaac tgccaggcta
      121 gagggagata gaggtgagcg gaacttaagg tggagcggtg aaatgcgttg atatcttaag
      181 gaacacctgt ggcgaaagcg gctcactgga tctcttctga cgctcatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009244
VERSION     KHUT01009244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           75 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggctttt
       61 tatgtcagtg gtgaaatcct agtgcttaac actagaactg ccattgaaac tggatagctc
      121 gagtcaagga gaggtaaaca gaatttatag tgtagtagtg aaatgcttag atactataag
      181 gaataccaat agcgaaggca gtttactggc cttgtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009245
VERSION     KHUT01009245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           88 g           51 t
ORIGIN      
        1 gacataggtg gcgagcgtta ttcggaatta ctaggcgtaa agcgagtgta ggcggacgct
       61 taagtctgac gttaaatctc cgggcttaac tcggagctgt cgtcggatac tgagcgcctc
      121 gaatggggta gggggcagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca gctgcctggg cctctattga cgctaagact cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01009246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009246
VERSION     KHUT01009246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           81 g           54 t
ORIGIN      
        1 gacgaaccgt acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgtgg
       61 aaagttggat gtgaaatacc tcagctcaac tgaggaactg cattcaaaac ttccatgcta
      121 gaggaagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009247
VERSION     KHUT01009247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtcaggg gtgaaatcct ccggcttaac cggagaacgg cttttgatac tggtggcctc
      121 gaggggggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009248
VERSION     KHUT01009248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           50 t
ORIGIN      
        1 tacgggagtg gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggcggccttg
       61 taagtctgtc gttaaagcgt ggagcttaac tccatttcag cgatggaaac tacaaggctt
      121 gagtgtggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg ctctgctggg ccataactga cgctcatgga cgaaagccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01009249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009249
VERSION     KHUT01009249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcacgta ggcgggtcgg
       61 tatgtcgggt gtgaaatccc cgggcttaac ctgggaagtg cattcgaaac tgccgacctt
      121 gagtacggga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009250
VERSION     KHUT01009250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           95 g           43 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtacctt gtgaaatccc ccggctcaac cggggaactg ctgggtagac tggcggtctt
      121 gagagaccta ggggccatcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggcgaaagca gatggctggg ggtcttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009251
VERSION     KHUT01009251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           90 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtgata
       61 taagttaggt gtgaaagtcc cgggcttaac ctggggattg cgcctgatac tgtgtcgcta
      121 gagtactgta gaggaaggtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatcggt ggcgaaggcg accttctgga cagatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009252
VERSION     KHUT01009252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          106 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccgc
       61 taagtccgtt gtgaaagacc gaggctcaac ctcggggtgt caacggagac tggtgggctc
      121 gagggtcgga gaggagcggg gaatccctgg tgtagcggtg gaatgcgtag agatcagggg
      181 gaacaccgat ggcgaaggca ccgctctggc cgatctctga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009253
VERSION     KHUT01009253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcacc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaacag cttctgatac tgggcgtctg
      121 gagggaggta ggggcacgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009254
VERSION     KHUT01009254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtctct
       61 taagtctgat gtgaaagtcc acggctcaac cgtgggattg cattggaaac tgggggactt
      121 gagtgcagaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009255
VERSION     KHUT01009255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtctggg gtgaaagccc ccggctcaac cggggaattg cctgggatac ttgcaagctc
      121 gagggaggta ggggcgatcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg gatcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009256
VERSION     KHUT01009256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           81 g           53 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttgc
       61 taagtctgct gttaaagctt gaagctcaac ttcaaaaatg caacagaaac tagtagactt
      121 gagtgtggta ggggcagagg gaatttccag tgaagtggtg aaatgcgtag atattggaaa
      181 gaccgccaaa ggcgaaggca ctctgctggg ccataactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01009257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009257
VERSION     KHUT01009257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           80 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggctag
       61 taagtctgtt gtgaaatctc cgggcttaac ccggaaattg cggtggatac tattagtctt
      121 gaatactgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cagatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009258
VERSION     KHUT01009258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           85 g           59 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta gacggtatgt
       61 taagtctgtt gttaaattcc ttagcttaac tgagggtatg cagcggaaac tgatgtgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009259
VERSION     KHUT01009259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c          101 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtagga
       61 tgggtgtggt gtgaaagccc cgggcttaac ctgggaaggg cactgcaaac tatgttactg
      121 gagtacatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009260
VERSION     KHUT01009260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          102 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtccgct gtgaaagcct gaggctcaac ctcagcgggt cggtggatac tgccagtctt
      121 gaaggcgtta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacacccgt ggcgaaggcg gtctcctggg acgttcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009261
VERSION     KHUT01009261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttaat
       61 taagtcaact gtgaaaaccc tgggcttaac ctgggaaagt cagttgatac tggtgagcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaggcg gctacctgga cagctactga cactgaagcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009262
VERSION     KHUT01009262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctata
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgtatggctt
      121 gaatcccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cgggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009263
VERSION     KHUT01009263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           89 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggtcggg
       61 taagtcggat gtgaaatctc actgcttaac ggtgaaatgg cattcgatac tgctcggcta
      121 gaggatcgga ggggagagcg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg gctctctgga agattcctga ctctgaaaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009264
VERSION     KHUT01009264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c           96 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggacccg
       61 ccagtctggg gtgaaatcgt tcggcttaac cggaccattg ccttggatac tgcgggtctc
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg gcgtgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009265
VERSION     KHUT01009265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           56 c          109 g           42 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctgat
       61 caggcctaac gtgaaagtcc tcggcttaac cggggagagt cgttggggac cggtcggctt
      121 gagggcggca ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccagctggg ccggctctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009266
VERSION     KHUT01009266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttcgc
       61 taagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cattcgaaac tgacgagctt
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009267
VERSION     KHUT01009267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacagagatg gcaaccgttg ttcggaatca ctgggcttaa agcgcacgta ggcgggatgg
       61 taagatgatc gtgaaatacc ctggcttacc cagggaactg cggtcattac tgccgttctg
      121 gagtacgaga ggggatagtg gaactcctag tggagcggtg aaatgcgttg atattaggag
      181 gaacgccggt ggcgaaagcg actatctggt tcgatactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009268
VERSION     KHUT01009268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtctgtg gtgaaatccg gcagctcaac tgtcgaattg ccgcggatac tgggtgtctc
      121 gaggaaggta ggggcatgcg gaactaacgg tggagcggtg aaatgcgttg atatcgttag
      181 gaactccggt ggcgaaggcg gcatgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009269
VERSION     KHUT01009269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaagccc tcggcttaac cggggaatgg cgcccaaaac taccgtgctg
      121 gaggaagaca gaggtgagcg gaactcaggg tggagcggtg aaatgcgttg atatcctgag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009270
VERSION     KHUT01009270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggcgggg
       61 taagtcgggt gtgaaatctc agggctcaac cctgaaattg cattcgatac tgcttcgctt
      121 gagctctgaa gaggaggttg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaaggccggt ggcgaaagcg aacctctggg cagcagctga cgctgtggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009271
VERSION     KHUT01009271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           84 g           55 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggtttgg
       61 taagtggatg attaaagacc gatgcccaac atcgggactg ttgtccatac tgcctgactt
      121 gaggatagca ggggtaagcg gaactttagg tgtaggagtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttactggg ctatttctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009272
VERSION     KHUT01009272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          100 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcgtgt
       61 tacgtcggcc gtgaaaacct actgcttaac ggtgggcttg cggtcgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009273
VERSION     KHUT01009273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           92 g           47 t
ORIGIN      
        1 gacgaaccaa gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggc
       61 caagtccgga gtgaaatctt tcggctcaac cggaaaagtg ctttggatac tggtcggctc
      121 gaagtaggta ggggttgcgg gaacagccag tggagcggtg aaatgcgttg atattggctg
      181 gaacgccggt ggcgaaagcg cgcaactgga cctctcttga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009274
VERSION     KHUT01009274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggctggg
       61 taagtttgat gtgaaatccc ggggctcaac ccttggactg cattgaatac tgctcggcta
      121 gagatctgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagatactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009275
VERSION     KHUT01009275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c          100 g           45 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtcagt
       61 taagtctgat gtgaaagccc tcggctcaac tggggaatgg cattggaaac tagctggctg
      121 gagtaccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gcttcctggc cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009276
VERSION     KHUT01009276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           97 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgcgg
       61 ttagtcaggc gtgaaactcc tgggctcaac ccaggaactg cgtttgatac ggccgtgctc
      121 gaggatggaa gagggccgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcggcctggt ccatttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009277
VERSION     KHUT01009277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatgct
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tgggtgtctt
      121 gagtgccgga gaggaagatg gaactgcacg tgtagcggtg aaatgcgttg agatgtgcag
      181 gaacaccaat ggcgaaagca gtcttctgga cggatactga cgctgagata cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01009278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009278
VERSION     KHUT01009278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcgggtctt
       61 taagtagaaa gtgaaaggtt ggggctcaac cccgacactg ctctctatac tggaggtctg
      121 gagtgccgga gaggaagatg gaactacacg tgtagcggtg aaatgcgttg agatgtgtag
      181 gaacaccaat ggcgaaagca atcttctgga cggttactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01009279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009279
VERSION     KHUT01009279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatga ctgggcgtaa agcgcgtgca ggcggcttcg
       61 caagtccgat gtgaaagccc agggctcaac cctggaagtg cattggaaac tacgaagctt
      121 gagtcctgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga caggtactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009280
VERSION     KHUT01009280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagtacgta ggcggtttgg
       61 taagacattg gttaaatctc cgggctcaac ccggaaccag cctgtgtgac tgccagacta
      121 gtcgagagca ggggcaagcg gaattgtcgg tgtaggagtg aaatccgttg agatcgacaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctctaggaga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009281
VERSION     KHUT01009281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggtctg
       61 taggtcagct gtgaaatccc agggcttaac cctggaatgg cggttgaaac catagatctt
      121 gaatacccaa gagagtggcg gaattccggg tgtagtagtg aaatacgtag atatccggag
      181 gaacaccaga ggcgaaggcg gctacttggt ggtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009282
VERSION     KHUT01009282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           90 g           44 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatca ttgggcgtaa agggtgccta ggcggcccgt
       61 caagtccggt gtgaaatccc tcggctcaac cgaggaactg catcggaaac tgacaggctt
      121 gagtgttgga gggggcaacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctggc caaacactga cgctgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009283
VERSION     KHUT01009283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          102 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccgg
       61 cgcgtcagag gtgaaagccc ccggctcaac cggggaaggg cctctgatac cgccggtctc
      121 gagggaggta ggggcggctg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg agccgctgga cctcatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009284
VERSION     KHUT01009284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c           89 g           50 t
ORIGIN      
        1 tacctgcagc ccgagtggtg ctcgttatta ttgggtctaa atcgtccgta gtcggctgag
       61 tagatgcccg ggtaaatcgc agcgcttaac gttgcgactt ccgggtagac cgctcggcta
      121 gggattggga gagccgcgag gtactctgag ggtaggggta aaatcctgta atccttgggg
      181 gaccaccagt ggcgaaggcg tcgcggtaga acaagtccga cgatcaggga cgaagccctg
      241 gggcgcaaac ggg
//
LOCUS       KHUT01009285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009285
VERSION     KHUT01009285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           99 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccgat
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaattg cacccgatac tggttggcta
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01009286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009286
VERSION     KHUT01009286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           96 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaga
       61 tgtgagtgat gtgaaagccc cgggcttaac ctgggaagtg catcgctgac tatttgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009287
VERSION     KHUT01009287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggagtca ctgggcttaa agggtgcgta ggcggcgccg
       61 caagtgtctt gtgaaatccc acggctcaac cgtggaatgg ctcgatagac tgcggcgctt
      121 gaggttggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccaattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009288             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009288
VERSION     KHUT01009288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           53 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgg
       61 ttagtgtgaa gtgaaatctc cagggctcaa cccggaaact gctttgcata ctgccagact
      121 agaggaatgc agaggtatgt ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcatactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01009289             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009289
VERSION     KHUT01009289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           38 c           79 g           60 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agagtgcgta ggtggtttaa
       61 taagttgctg gtggaaaatc ggggctcaac ctcgtggaag tcagtaatac tgttgaacta
      121 gagggtcgga gaggttaacg gaatggttca tgtaggggta aaatccataa atatgaatta
      181 gaacaccaat tggcgaaggc agttaactat tcgactcctg acgttgaggc acgaaagcgt
      241 gggtagcaag acgg
//
LOCUS       KHUT01009290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009290
VERSION     KHUT01009290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggcggccaat
       61 tatgtcgaat gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tggttggctt
      121 gagtaccgga gaggagggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg accctctgga cggatactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009291
VERSION     KHUT01009291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           96 g           52 t
ORIGIN      
        1 tacggaggcc ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtattt
       61 taagtctggt gtgaaaggcg tgggctcaac ccgcggactg cattggatac tgggatactt
      121 gaggactgga gaggagaatg gaattcacgg tggagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaggcg attctctgga cagtacctga cgctgaggca cgaaggctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01009292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009292
VERSION     KHUT01009292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           90 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttat
       61 cacgtcggct gtgaaatccc gaggcttaac ctcgggcctg cagtcgatac gggttgacta
      121 gagtgatgca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg cattaactga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009293
VERSION     KHUT01009293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           75 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggacga
       61 taagtcagtg gtgaaatccc atagcttaac tatggaattg ccattgatac tgtcgacctt
      121 gagtacaaac gatgtgggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat agcgaaggca gctcacaaga atgtaactga cgctgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009294             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009294
VERSION     KHUT01009294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           93 g           46 t
ORIGIN      
        1 cacgtgggag gcgagcgttg tccggattca ttgggcgtaa agagcgcgca ggcggctaag
       61 caagtcggtt gtgaaatccc gttggctcaa ccacggaact gcatccgata ctgcttggct
      121 agaggtaggt aggggagatc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggatctctgg gccttacctg acgctcaggc gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009295             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009295
VERSION     KHUT01009295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggctttg
       61 taagtcactt gtgaaatctc tgagcttaac tcggaacggt caagtgatac tgcaatgcta
      121 gagtgcggaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggtgaagacg ggttgctggg ccgacactga cgctgacagc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009296
VERSION     KHUT01009296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgtgtgta ggcggcttct
       61 caagtcggat gtgaaatccc agggctcaac cctggaagtg cattcgaaac tgggtagcta
      121 gagtactgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg accttctgga cagatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009297
VERSION     KHUT01009297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           96 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctcg
       61 tttgtctggg gtgaaagccc ggagcttaac ttcggaagtg cctcagaaac ggcgagactt
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtgactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009298
VERSION     KHUT01009298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           83 g           60 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggcttga
       61 taagtcagga gtgaaatccc ggagcttaac tccggaattg cttttgaaac tattaggcta
      121 gagtatgtta gaggaaatcg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gatttctggg acattactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009299
VERSION     KHUT01009299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           92 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcgggcccg
       61 tatgtcgatt gtgaaatccc cgggcttaac ctgggaattg cagtcgaaac tgcgggtctt
      121 gagtgcttaa gaggatggcg gaattcccgg tgtagaagtg aaattcgtag atatcgggaa
      181 gaacacctga ggcgaaggcg gctgtctggt gatgcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009300
VERSION     KHUT01009300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           51 c           77 g           51 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggaatta ctgggcgtaa agcgtgagca ggtggtaaat
       61 caggtcagat actaaatctt acggctcaac cgtaagctgc gatctgaaac cggtttacta
      121 gaggtcggta gaggcaagcg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccgcacctga cactcagtca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009301             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009301
VERSION     KHUT01009301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           46 t
ORIGIN      
        1 aacgtaggac gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggcttgt
       61 taagtcgtgc gtgaaagctc cctggctcaa ccgggagagg tcgtacgaaa ctggcgagct
      121 tgagggcagc agaggagtgt ggaattccgg gtgtagtggt gaaatgcgta gatatccgga
      181 ggaacaccag tggcgaaggc ggcactctgg gctgtacctg acgctaagac gcgaaagcat
      241 gggtagcgaa cggg
//
LOCUS       KHUT01009302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009302
VERSION     KHUT01009302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcct
       61 ttagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tggggatatc
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009303
VERSION     KHUT01009303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           85 g           51 t
ORIGIN      
        1 tacgaaggga gctagcgtta ctcggaataa ctgggcgtaa agcgcatgta ggcggaacta
       61 caagtcaggg gtgaaatccc gaggctcaac ctcggaattg cctttgaaac tgtagttctt
      121 gagtgtgaca gaggatggtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg accatctggg tcacaactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009304
VERSION     KHUT01009304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           78 g           59 t
ORIGIN      
        1 tacgtaggtc gcgagcgtta tccggaatta ttgggcgtaa agagttcgta ggcggccaag
       61 ccagtcttat gtgaaagcca acggcttaac tgttgaattg cataagatac tatttggcta
      121 gaatttagga ggggaaactg gaattccatg tgtagcggtg gaatgcgtag atatatggag
      181 gaacaccaat agcgaaggca ggtttctgga ctaaaattga cgctgaggaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009305
VERSION     KHUT01009305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           82 g           52 t
ORIGIN      
        1 gacagaggtc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggattag
       61 caagtacttt gtgaaatccc atggcttaac catggaactg cttggtatac tgctagtctt
      121 gaggcatcta gaggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgcccaa ggtgaaaaca ggttgctggg gatgtactga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009306             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009306
VERSION     KHUT01009306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctat
       61 taagtcggat gtgaaatctc cacggctcaa ccgggaaaca gcatccgaaa ctggtagcct
      121 tgaggtatgg agagggaagt ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacacccg tggcgaaggc ggcttcctgg ccatatcctg acgctgaggt gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01009307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009307
VERSION     KHUT01009307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           96 g           39 t
ORIGIN      
        1 gacagggggc ccgagcgtta ctcggaatca ctgggcttaa agggtgcgta ggcggtctcg
       61 caagcgtctt gtgaaatccc cgggctcaac ccgggaacgg cagggcgaac tgcgggactt
      121 gaggaaacta gaggccggca gaacggtagg tggagcggtg gaatgcgtag agatctaccg
      181 gaatgccaaa ggtgaagaca gccggctggg gttttcctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009308
VERSION     KHUT01009308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           91 g           44 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggccgtccgc
       61 taagtcaggc gtgaaatccc ccggctcaac cggggaattg cgcctgatac tggtggactc
      121 gaggtcggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgaatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009309
VERSION     KHUT01009309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           59 c          110 g           35 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggagtta ctgggcgtaa agcgcgcgta ggcggcggag
       61 cgcgtcgcac gtgaaagccc ccggctcaac cggggagggt cgtgcgagac ggcttcgctg
      121 gaggcagcga gaggctggtg gaattcccgg agtagtggtg aaatgcgtag atatcgggag
      181 gaacaccgga ggcgaaggcg gccagctggc gctgacctga cgctgaggcg cgaaggcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009310
VERSION     KHUT01009310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          100 g           46 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggctgtc
       61 taagactggt gtgaaatctc ccggctcaac cgggagggtg cgccggagac tgggcggctc
      121 gagtgcggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagacg gctctctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009311
VERSION     KHUT01009311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           81 g           53 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ctgggcgtaa agggtgtgta ggcgacattt
       61 caagtccact gttaaatatc ccagcttaac tgggaaggtg cagtggaaac tgattcgtta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gcttcttagt ccacacctga cgctgataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009312             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009312
VERSION     KHUT01009312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           81 g           57 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcatga
       61 caagttatta gtgggaaatc ggggctcaac ctcgtggaag ttagtaatac tgttatgcta
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgcaacg atatatggat
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009313
VERSION     KHUT01009313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtttgat gtgaaagctc cgagcttaac tcggaaattg cattgaatac tatcccgctc
      121 gagggttgta ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009314
VERSION     KHUT01009314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggtggcctgt
       61 tatgtcggtc gtgaaagccc aaggctcaac cttggaagtg caaccgaaac tggcaggcta
      121 gagttctgaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcgga ggcgaaggcg gccatctggg aagcgactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009315
VERSION     KHUT01009315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           86 g           52 t
ORIGIN      
        1 tacgaggggt gcgagcgtta ttcggaatta ttgggcgtac agggtgcgta gacggcatat
       61 taagtcaact gttaaattcc ccggcctaac tggggtccag cagtagatac tgatgagcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009316
VERSION     KHUT01009316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           85 g           51 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcttag
       61 caagttgggt gtgaaatacc tcggctcaac cggggaatgg cgctcaaaac tgctgagctg
      121 gaggaagata gaggtgagcg gaacttaagg tggagcggtg aaatgcgttg atatcttaag
      181 gaacacctgt ggcgaaagcg gctcactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009317
VERSION     KHUT01009317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacaac
       61 caagtcagag gtgaaatcct gcggcttaac cgtagaagag cccctgatac tggttgtctc
      121 gagggagata ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009318
VERSION     KHUT01009318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcccgt gtgaaagccc ccggctcaac cggggaactg cacgggaaac tgggaggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009319
VERSION     KHUT01009319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           83 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggtatg
       61 taagtcagat gtgaaagccc ggagctcaac tccggaacag catttgaaac tgcatgtcta
      121 gagggtaggc ggagaaaata gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggcgaaggcg attttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01009320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009320
VERSION     KHUT01009320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggcctgc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaacag cttctgatac tggcgggctc
      121 gagggaggta ggggcagatg gaacttcggg tggagcggtg aaatgtgtag atatccgaag
      181 gaacgccggt ggcgaaagcg atctgctgga tctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009321
VERSION     KHUT01009321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtccta
       61 taagtcagcg gtgaaatccc gacgctcaac gtcggaattg ccgttgatac tgcaggactt
      121 gagttcgatc gaggtcgttg gaacgcgtag tgtagcggtg aaatgcttag atattacgca
      181 gaacaccgat tgcgaaggca gatggctagg tcgcgactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01009322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009322
VERSION     KHUT01009322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtagat
       61 taagttggaa gtgaaatccc tgggcttaac ctaggaatgt ctttcaaaac tggtttgcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009323
VERSION     KHUT01009323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           85 g           58 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatga ttgggcgtaa agggtgcgta ggcggtgtgt
       61 taagtctatt gttaaattct tcagcttaac tggagacctg caatagaaac taatgtgcta
      121 gagggtggga gagagaagtg gaattctcgg agtagcggta aaatgcttag atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009324
VERSION     KHUT01009324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacggagagg gcaagcgttg ttcggattta ctgggcataa agcgcacgta ggcggaccta
       61 caagtcggtt gtgaaatccc ggggctcaac cccggaactg catccgatac tataggtcta
      121 gaggatgaaa ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggt tcatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009325
VERSION     KHUT01009325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           90 g           49 t
ORIGIN      
        1 cacgtactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccgat
       61 taagtagggt gtgaaatccc tcggctcaac cgaggaatgg cgctctaaac tggtcggctt
      121 gagtgagata ggggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt agcgaaagcg gcacactggg tctcaactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009326
VERSION     KHUT01009326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccga
       61 taagtcggtt gtgaaagtca agggctcaac ccttgaatgc cgatcgatac tgtcgggcta
      121 gagtccggaa gaggtgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcactggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009327
VERSION     KHUT01009327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggtatag
       61 caagtcgagt gtgaaagccc cgagcttaac ttgggaattg cgctcgaaac tactgtactg
      121 gagtatggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccttctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009328
VERSION     KHUT01009328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           90 g           56 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtctgat gttaaagatc ggggctcaac cccgggagtg cattggatac tggcagactc
      121 gagtatggta gaggcgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgcaagcg actcgctggg ccattactga cactgaggaa cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01009329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009329
VERSION     KHUT01009329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           87 g           50 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggtgaga
       61 taagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac tatctcgcta
      121 gaggtcagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggtgaagacg gttttctggg ctgatactga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009330
VERSION     KHUT01009330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggcggcccaa
       61 aaagtcccgt gtgaaatccc ttggcttaac caaggaagtg cgcgggaaac tgaagggctt
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009331
VERSION     KHUT01009331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           86 g           55 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggagcga
       61 taagtgcctt gtgaaatccc atggcttaac catggaattg ctcggtagac tatcgttctt
      121 gaggtaggta gaggtcactg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgat ggtgaagaca ggtgactggg cctattctga cgctgatgca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009332
VERSION     KHUT01009332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           83 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggctgcgccg
       61 taagtcagat gtgaaagctc agggctcaac cctgaaattg catccgatac tgcggtgctt
      121 gagaaatgaa ggggagtcta gaattttcgg tgtagcagtg aaatgcgtag atatcgaaag
      181 gaataccaaa ggcgaaggca ggactctggg cattttctga cgctgatgca cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009333
VERSION     KHUT01009333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           81 g           52 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtcctgt
       61 taagtctccg gtgaaatccc tcggctcaac tgaggaattg tcggagaaac taacaggatt
      121 gagtgtgaca gaggcagacg gaattcctgg tgtagcggta aaatgcgttg atatcaggag
      181 gaacaccaat ggcgaaggca gtctgctggg tcacaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009334
VERSION     KHUT01009334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           93 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatga ctgggcgtaa agggtgtgta ggcggttaga
       61 cacgtcagat gtgaaatccc cgggcttaac ctgggaatgg catttgatac ggtctagcta
      121 gagattggta gagggtagtg gaattgcgag tgtagaggtg aaattcgtag agattcgcag
      181 gaacaccggt ggcgaaggcg gctacctggg ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009335
VERSION     KHUT01009335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtgtgt
       61 taagtttgtc gttaaagact ctagcctaac tggagggttg cgatgaaaac tagcgcgcta
      121 gaggatgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009336
VERSION     KHUT01009336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttttg
       61 caagtcgagt gtgaaagccc cgggctcaac ctgggaactg cattcgagac tgcattactc
      121 gagtatggga gagggcagtg gaatttcggg tgtagcggtg aaatgcgtag atatccgaag
      181 gaacatcagt ggcgaaagcg actgcctggc ccaatactga cactcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009337
VERSION     KHUT01009337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggctggg
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaactg cattcgatac tgcccggcta
      121 gagtatggta gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009338
VERSION     KHUT01009338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           80 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggtcaga
       61 taagtcagtg gtgaaagccc gcggctcaac cgtggaattg ccattgaaac tgcctgactt
      121 gaatacagtt gaagtggatg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccaat tgcgaaggca gttcactaag ctgtcattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009339
VERSION     KHUT01009339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgaacgta ggcggctatg
       61 taagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tgcatggctt
      121 gagtgtcgga gaggatagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgaacactga cgctgaagtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009340
VERSION     KHUT01009340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           38 c           85 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttat
       61 taagttagat gtgaaaattc tgggcttaac ctaggacgtg catttaagac tgatgagcta
      121 gagtattgta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga caaatactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009341
VERSION     KHUT01009341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           82 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatt
       61 caagtcagct gtgaaatccc cgggcttaac ctgggaactg cggttgaaac tagataactt
      121 gagtttggga gagggaagtg gaattccatg tgtagcagtg aaatgcgtag atatatggag
      181 gaacaccgat ggcgaaggca gcttcctggc ccaaaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009342
VERSION     KHUT01009342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggcgtaa agagttcgta ggcggcatgt
       61 aaagtcaggt gttaaaggct gaggctcaac ctcagtatgg cacttgatac ttgcaagcta
      121 gaatgcggta gaggtaaagg gaattcctgg tgtagcggtg aaatgcttag atatcaggag
      181 gaacatcggt ggcgtaagcg ctttactggg ccgtaattga cgctgaggaa cgaaagccag
      241 ggtagcaaat ggg
//
LOCUS       KHUT01009343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009343
VERSION     KHUT01009343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           93 g           43 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggatcaa
       61 aaagtcaggg gtgaaatccc acggcttaac cgtggaagtg cctttgatac tgttgatctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009344
VERSION     KHUT01009344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccga
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagcg ccttggatac ttttggcctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009345             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009345
VERSION     KHUT01009345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ttgggcttaa agggcgcgta ggcggcaagt
       61 taagtggagg gtgaaatcct ggcggctcaa ccgcgggatt gccttgccat actggcttgc
      121 tcagagggag gtaggggtcg gcggaacttc cggtggagcg gtgaaatgtg ttgagatcgg
      181 aaggaacgcc cgtggcgaaa gcggccgact ggacctttcc tgacgctgag gcgcgaaagc
      241 caggggagca aacggg
//
LOCUS       KHUT01009346             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009346
VERSION     KHUT01009346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           56 c          107 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattca ttgggcgtaa agggcgcgta ggcggcccgg
       61 cgcgtcatgc gtgaaatctc ccggctcaac tgggaggggt cgtgtgatac ggtcgggctg
      121 gagcgaggta gggggcagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctgga cctttttagc tgacgctgag gcgcgaaagc
      241 gtggggagcg atccgg
//
LOCUS       KHUT01009347             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009347
VERSION     KHUT01009347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           77 g           61 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcataa
       61 caagttatta gtgggaaatc ggggctcaac ctcgtggaag ctagtaatac tgttttgctt
      121 gagtacagga gaggttaacg gaactctttg tgtaggggta aaatcccatg atataaagat
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009348             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009348
VERSION     KHUT01009348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           39 c           76 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtttga
       61 taagttagcg gtggaaaatc gaggctcaac ctcgtggaag tcgctaatac tatcaaacta
      121 gagtgcagga gaggttaacg gaactctatg tgtaggggta aaatccgtag atatatagat
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcatgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009349
VERSION     KHUT01009349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 tgcgtcggct gtgaaaaccc acagcttaac tgtgggcctg cagccgatac gggcgagcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009350
VERSION     KHUT01009350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           84 g           57 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtggatctt
       61 taagtcggtg gtgaaatcct gcagcttaac tgtagaactg cctccgatac tgaagatctt
      121 gagtacggca gagggagatg gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaacaccagt tgcgaaggcg gtctcctggt ccgaaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009351
VERSION     KHUT01009351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          101 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggcctgt
       61 taagtcaggg gtgaaacccc ggggctcaac cccgggcctg cctctgagac tggcgggctt
      121 gagtgctcca gagggcagcg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg gagtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009352
VERSION     KHUT01009352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           95 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcacgta ggcggcgatg
       61 ttagtctgat gtgaaagacc tgggcttaac ctgggatgtg cattggaaac ggcatggcta
      121 gagtatggga gagggaagtg gaatttccgg agtagcggtg aaatgcgtag atatcggaaa
      181 gaaccctcgt ggcgaaggcg actttctggc ccaaaactga cgctgaggtg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009353             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009353
VERSION     KHUT01009353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           89 a           30 c           65 g           68 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcaaattta ctgggcgtaa agggcacgta ggtggtttgt
       61 tatgtgtact gttaaattgc aaagcttaac tttgtaaaat agtatataca gctaaacttg
      121 agttaggtag aaggtagtag aatttctaat gtaaaggcga aatttgataa aattagagag
      181 aataccaaag gcgtaggcaa ctacctagac cttaactgac attgaggtgc gaaagcatgg
      241 ggagcaaata gg
//
LOCUS       KHUT01009354             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009354
VERSION     KHUT01009354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           44 t
ORIGIN      
        1 tacggggggg caagcgttgt tcggaattac tgggcgtaaa gggctcgtag gcggccaact
       61 aagtcaaacg tgaaatccct cggcttaacc ggggaactgc gcttgatact ggatggcttg
      121 agattgggag agggatgcgg aattccaggt gtagcggtga aatgcgtaga tatctggagg
      181 aacaccggtg gcgaaggcgg catcctggac caacactgac gctgaggagc gaaagccagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01009355             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009355
VERSION     KHUT01009355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttatt
       61 taagttgggt gtttaatccc ggggctcaac ctcgggtcgc acccaaaact gggtaacttg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009356             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009356
VERSION     KHUT01009356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           43 t
ORIGIN      
        1 tacggggggg caagcgttgt tcggaattac tgggcgtaaa gggcgcgtag gcggcctact
       61 aagtcggacg tgaaatccct cggctcaacc gaggaactgc gtccgatact ggcaggctgg
      121 aatccgggag agggatgtgg aattccaggt gtagcggtga aatgcgtaga tatctggagg
      181 aacaccggtg gcgaaggcgg catcctggac cggtattgac gctgaggcgc gaaagctagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01009357             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009357
VERSION     KHUT01009357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           35 c           77 g           61 t
ORIGIN      
        1 gacgaagggg gcgggcgtta cccatgatga ctgggcgtaa agagtccgta ggcggctttt
       61 caagttaaaa gtaaaatcaa aaagcttaac tttttgaggc ttttaatacg ttagagcttg
      121 gagtttgaag ggagatagta gaatttcata tggaggggtg aaatccgtag aattatgaag
      181 gaatactgat ggcgaaggcg actatctatt tcaatctgac gctgagggac gaaagcatgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009358             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009358
VERSION     KHUT01009358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttttt
       61 taagtctggt gtttaagccc agggctcaac cctggttcgc accggaaact ggaagacttg
      121 agtgcaggag aggaaagcgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg ctttctggac tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009359             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009359
VERSION     KHUT01009359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ctgggcgtaa agggtgcgta ggtggctgat
       61 caagccaggg gtgaaaggca ttggctcaac caatgtaagc ctttggaact gatcggcttg
      121 agtgcaggag aggtaagtgg aattcctagt gtagaggtga aattcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga cttactggac tgtaactgac actgaggcac gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01009360             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009360
VERSION     KHUT01009360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttctt
       61 taagtccggt gtttaagccc ggggctcaac ctcgggacgc acgggaaact gggggactgg
      121 agtgcaggag agggaagcgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg cttcctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009361
VERSION     KHUT01009361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tgcagagctc
      121 gagtgccaga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccagt ggcgaaggcg gccacctgga tggtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009362             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009362
VERSION     KHUT01009362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatga ttgggcgtaa agcgcgcgca ggcggcgagg
       61 taagttgggt gtttaaacct agggctcaac cttgggtcgc atccaaaact gcatcgcttg
      121 agtgcagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtcactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009363
VERSION     KHUT01009363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggcttaa
       61 caagtcagat gtgaaatctc agggcttaac tctgaaagtg catttgaaac tgttaggcta
      121 gagtgcagta gaggatggcg gaatacccag tggagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gccatctggg ctgtaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009364             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009364
VERSION     KHUT01009364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agggagcgca ggtgggaaag
       61 taagtcagtc ttaaaagttc ggggctcaac cccgtgatgg gattgaaact acttttcttg
      121 agtgcaggag aggaaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcgg ctttctggac tgtaactgac actgaggctc gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01009365             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009365
VERSION     KHUT01009365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           96 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtgagt
       61 taagttgtgg gtataaggca ttggctcaac caatgtacgc ctgcaaaact ggctcgcttg
      121 agtgcaggag aggggagtgg aattcccggt gtagcggtgg aatgcgtaga tatcgggagg
      181 aacaccagtg gcgaaggcga ctctctggac tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcgaaca gg
//
LOCUS       KHUT01009366             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009366
VERSION     KHUT01009366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggtggctatg
       61 taagtctgat gttaaagccc gaggctcaac ctcggttcgc attggaaact gtgtagcttg
      121 agtgcaggag aggaaagtgg tattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac actgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009367             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009367
VERSION     KHUT01009367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           33 c           75 g           69 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggattta ttgggtgtaa agggtgcgta gatggcttag
       61 taagtctcag gtaaataatg gagctcaact ccattagtct tgatgatact gctttgcttg
      121 agaatggaag aggtaaacgg aatttctagt gtagttgtga aaagcttaga tattagaagg
      181 aacaccagag gcgaaagcgg tttactatta catttctgac attgaggcac gaaagcgtgg
      241 ggagcaaatg gg
//
LOCUS       KHUT01009368             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009368
VERSION     KHUT01009368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           32 c           76 g           65 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa aggatgtgta ggctgttttg
       61 aaagttgcta gtggcaaatt ggaagtaatt tcatggaagc tagtaatact tcttaactag
      121 agctcaggag aggttaatgg aatttcatgt ggagcggtaa aatgtgacga tatatggaag
      181 aacaccagag gcgaaggcga ttaactattc tgttgctgac gttgagacat gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009369
VERSION     KHUT01009369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           95 g           42 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccatg
       61 ccagtcgggt gtgaaaatcc ggggctcaac cccggacctg catccgatac tgcatggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009370             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009370
VERSION     KHUT01009370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggccttt
       61 taagtctggt gtttaaacct ggggctcaac cccgggtcgc acgggaaact ggaaggctgg
      121 agtacaggag agggaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga cttcctggcc tgaaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009371             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009371
VERSION     KHUT01009371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttggt
       61 taagtctgat gtttaagccc ggggctcaac cccggttcgc atcggaaact ggctaacttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009372
VERSION     KHUT01009372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           83 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggacctg
       61 taagtcagtg gtgaaagcct gcggcttaac cgtagaacag ccattgatac tgcaggtctt
      121 gaatcaggtc gaggtagttg gaatacgtag tgtagcggtg aaatgcttag atattacgta
      181 gaacacctat tgcgaaggca gattactagg cctgaattga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009373
VERSION     KHUT01009373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacagggggt gcaagcgttg ctcggaatta ctgggcgtaa agcgagcgca ggcggccgat
       61 taagtcagag gtgaaagccc ggggctcaac cccggaaatg cctttgaaac tggtaggctt
      121 gaatgcagga gagggtcgcg gaattcttgg tgtagaggtg aaattcgtag atatcaggaa
      181 gaacaccagt ggcgaaagcg gcgacctggc ctgacattga cgctcatgct cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009374
VERSION     KHUT01009374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcgggctac
       61 aaagtccgat gtgaaatccc gaagcttaac ttcggaactg cattggatac tggtagcctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009375
VERSION     KHUT01009375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggaccga
       61 caagttggat gtgaaatccc tcggcttaac cgaggacgtg cattcaaaac tgtcggcctt
      121 gagtaccaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009376
VERSION     KHUT01009376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           96 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggattta ttgggtgtaa agggtgcgta ggcgggacga
       61 taagtccgag gtgaaagccc gcagcttaac tgcggaactg ccttggatac tgtcgacctt
      121 gagtcccgga ggggtggctg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgga ggtgtagacg ggtcactgga cgggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009377
VERSION     KHUT01009377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggctggg
       61 taagtttgat gtgaaagccc cgggcttaac ccgggaatgg cattgaatac tattcggctg
      121 gaggattgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cactgaggca cgaaagccaa
      241 gggagcaaac agg
//
LOCUS       KHUT01009378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009378
VERSION     KHUT01009378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          101 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggccatg
       61 taagtacgtt gtgaaatctc cctgctcaac ggggagggtg ctgcgtaaac tgcacggctt
      121 gaggacggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009379             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009379
VERSION     KHUT01009379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           54 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtgagta ctgaaatctc cagggcttaa cccggaaact ggttctcata ctggcagact
      121 tgaggaaggc agaggtaact ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaactccaa tggcgaaggc aggttactgg gccttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01009380             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009380
VERSION     KHUT01009380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttatg
       61 taagtctgat gttaaagccc ggggctcaac ctcggtccgc attggaaact gtgtaacttg
      121 agtgcagaag aggaaagtgg tattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009381             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009381
VERSION     KHUT01009381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctatg
       61 taagtctggt gttaaagccc ggggctcaac cccggttcgc atcggaaact gcgtagcttg
      121 agtgcagaag aggaaagcgg tattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg ctttctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009382             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009382
VERSION     KHUT01009382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           91 a           32 c           64 g           65 t
ORIGIN      
        1 tacgggaggg gcaagcgtta ttcggcatga ctgggcgtaa agtgttcgta gacggtaaaa
       61 caagttactt gttaaaaatt aaagcctaac tttaaaatgg cttgtaatac tattttactt
      121 gagttttata cagaagagta gaattttaca tgaaagggtg aaatctacgg atatgtaaag
      181 gaataccaaa agcgaaggca actctttagt aaaaactgac gttgagggac gaaagtatgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009383
VERSION     KHUT01009383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgtgta ggcggtaata
       61 tgtgtgtgat gtgaaagccc tgggcttaac ctaggaagtg cattgcaaac tatattactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009384
VERSION     KHUT01009384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           87 g           57 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtctggt gttaaatact gaagctcaac ttcggaactg cactggatac tggcagactt
      121 gagtacggta gaggcgagcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaagcg gctcgctggg ccgttactga cactgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01009385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009385
VERSION     KHUT01009385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           93 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtaac
       61 caagtccggg gtgaaagcct ccagcttaac tggagaagtg cctcggatac tggtcgtctc
      121 gaggagggta ggggcatctg gaactagcgg tggagcggtg aaatgcgttg atatcgctag
      181 gaactccgat ggcgaaggca aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009386
VERSION     KHUT01009386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcttta
       61 tgtgtcgaat gtgaaatacc tgggcttaac cgaggaagtg cattcgaaac tgtagagctt
      121 gaatcccaaa gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg accaactggt tggagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009387
VERSION     KHUT01009387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 gacgagctct ccaaacgtta ttcggtatta ctgggcttaa agagttcgta ggcggtcgcg
       61 taggtgaggt gtgaaagccc tcggcttaac cgaggaattg cgcttcaaac cgcgtggcta
      121 gaggaaggta ggggtatgcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gcatactgga ccttatctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009388
VERSION     KHUT01009388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           84 g           58 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcagag
       61 taagttaggc gtgaaatgct aaagctcaac tttggcactg cgcttgatac tgctttgctc
      121 gagtgctaga gaagaagctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg agcttctaga tagttactga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009389
VERSION     KHUT01009389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           84 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctg
       61 taagtcagtg gtgaaatact ctagcttaac tagaggggtg ccattgatac tgcagggctt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaactccgat agtgaagaca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009390
VERSION     KHUT01009390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttga
       61 taagtgagct gtgaaatccc cgggcttaac ctgggaactg cagcttagac tgtgagactg
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgaaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009391
VERSION     KHUT01009391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaataa ctgggcgtaa agcgcatgta ggcggatctt
       61 taagtcagag gtgaaatccc tgggctcaac cccggaattg cctttgatac tggagatctg
      121 gagtctggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc ccgagactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009392
VERSION     KHUT01009392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttatc
       61 taagtctggt gttaaagccc gaagctcaac ttcggcatgg cattggaaac tgggtagcta
      121 gagtgtggta gaggtatcgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgatactggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01009393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009393
VERSION     KHUT01009393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttagat gtgaaagtcc tgggctcaac ctggggactg cgtctgatac tgtcaggcta
      121 gagttctgta gaggaaggtg gaatttccgg agtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaagcg gccttctgga cagaaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009394
VERSION     KHUT01009394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           84 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggttgga
       61 taagtttttt gtgaaagctc ccggcttaac tgggagaggc caagaaatac tgtctgactc
      121 gagtgtggta ggggaaactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctat ggcgaaagca agtttctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009395
VERSION     KHUT01009395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           84 g           48 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtccgca gccgatcatg
       61 ttagtctggt gttaaatccc ggggcccaac cccggaatcg tgccggaaac tgcatgaatt
      121 gagttataca gaggcacctg gaatatcgtg tgtaggggta aaatccgttg atccacggta
      181 gaacgccaaa agcgaaggca gggtgctggg tatgtactga cggtcaggga cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009396
VERSION     KHUT01009396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c           99 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcc
       61 caagtctgtg gtgaaatccc ccggctcaac cggggaactg ccgcggatac tgggagcctc
      121 gaggggtgta ggggcatgcg gaacttcggg tggagcggtg aaatgcgtag agatccgaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009397
VERSION     KHUT01009397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           92 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtccac
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaatgg cacttgatac tggtgggctc
      121 gaggatgaga tgggtaggcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcctactgga ccatcactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009398
VERSION     KHUT01009398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctctt gtgaaagctc ccggcttaac tgggagaggc caagggaaac tgtcgggcta
      121 gagtgtggca gaggatgctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggcatctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009399
VERSION     KHUT01009399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggtggatatt
       61 taagtgggat gtgaaatccc cgggcttaac ctgggggctg cattccaaac tggatgtcta
      121 gagtgcagga gaggagagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actctctgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009400
VERSION     KHUT01009400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatatt
       61 taagtcagat gtgaaattcc cgggctcaac ttgggagctg catttgaaac tggatatcta
      121 gagtgcggga gaggagagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actctctgga ccgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009401             250 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009401
VERSION     KHUT01009401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>250
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           86 g           48 t
ORIGIN      
        1 gacggggggg caagtgttct ttagaatgac tgggcgtaaa gggcacgtag gcagtgaatc
       61 aggttgaaag tgaaagtcgc taaaaagtgg cggaatgctc tcgaaaccaa ttcacttgag
      121 tgagacagag gagagtgaaa tttcgtgtgt aggggtgaaa tccatagatc tacgaaggaa
      181 tgccaaaagc gaaggcagct ctctgggtcc ctaccgacgc tggggtgcga aagcatgggg
      241 agcgaatagg
//
LOCUS       KHUT01009402             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009402
VERSION     KHUT01009402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agggtgcgta ggcggccttt
       61 taagtcagat gtgaaaggct acggcttaac cgtagttagc atttgaaact agaaggcttg
      121 agtgcaggag aggagagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctctctggac tgcaactgac gctgaggcac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009403             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009403
VERSION     KHUT01009403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcgat
       61 gaagtctggg ggttaagccc ggggctcaac cccgggtcat cctggaaact catcgactgg
      121 aggacagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctctctggtc tgtgactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01009404             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009404
VERSION     KHUT01009404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           78 g           59 t
ORIGIN      
        1 tacgaaggtc gcaagcgtta tccggagtta ctgggcgtaa agagttcgta gctggtctag
       61 taagtctgat tttaaagact ctgtctcaag cagaggaagg ggtcagatac tgctattctg
      121 gagataactg ggggttagtg gaattaccgg tggaggggtg aaatccgttg atatcggtag
      181 gaacgccaaa agcgaaagca actaactatg gttttctgac agtcaagaac gaaagctcgg
      241 ggatcaaacg gg
//
LOCUS       KHUT01009405             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009405
VERSION     KHUT01009405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           77 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtacgta ggcggttttg
       61 taagtcagac gttaaagact atggctaaac catggtacgc gtttgaaact gcgagacttg
      121 aatatattcg aggtgcatgg aatgcttcat gtagcggtga aatgcataga tatgaagtag
      181 aacaccaaaa gcgaaggcag tgcactagga tatcattgac gctgaggtac gaaagcgtgg
      241 gtagcgaaca gg
//
LOCUS       KHUT01009406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009406
VERSION     KHUT01009406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctca
       61 tttgtccgag gtgaaagcct tgagcttaac tcaagaaggg cctcggaaac ggtgggacta
      121 gagtcctaga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaagcg gcgacctgga taggaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009407
VERSION     KHUT01009407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           85 g           47 t
ORIGIN      
        1 tacagagggc gcaaacgttg ctcggaatta ctgggcgtaa agcgtgcgta ggcggacgaa
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaactg cattcgaaac tgctcgtcta
      121 gagtactgga gaggaaagcg gaattcttgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagttactga cgctcagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009408
VERSION     KHUT01009408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcctcg
       61 catgtcgaat gtgaaagccc acctcttaaa ggtggaattg cattcgaaac tgcaaggcta
      121 gagttccgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009409
VERSION     KHUT01009409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c          102 g           35 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgca ggcggtatgg
       61 agcgtcgctg ctgaaagcgc ccggcttaac cgggcgaggc gtggcgatac gaccaaacta
      121 gaggctcgga gagggtggtg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacctgt ggcgcaagcg accacctggc cgagacctga cgctgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009410
VERSION     KHUT01009410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           94 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcgt
       61 taagtcagct gtgaaagccc tcggcttaac cgaggaaggg cagctgaaac tggcgtgcta
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009411
VERSION     KHUT01009411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttt
       61 taagacatct gtgaaatacc tgggcttaac ctgggcaggt cagatgagac tgagagactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009412
VERSION     KHUT01009412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          106 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgtc
       61 caggcccgcg gtgaaagccc ccggctcaac cggggaaggg ccgcggggac cggacgtctg
      121 gagtgaggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcgactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009413
VERSION     KHUT01009413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 tacagaggcc tcaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggcgatctt
      121 gggtcattca ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggttgctggg gatgtaccga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009414
VERSION     KHUT01009414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcggactgc
       61 atagtctggg gtgaaatccc acggctcaac cgtggaactg cctcagatac tggcggtctc
      121 gagggaggta ggggcgagcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaagcg gctcgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009415             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009415
VERSION     KHUT01009415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           80 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggcttg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaatag catttgaaac tgcaagtcta
      121 agagggtaga tggagaaaat ggaattccac aagtagcggt gaaatgcgta gatatgtgga
      181 agaacaccag tggcgaaggc ggttttctaa tttatacctg acgctaaagc gcgaaagcta
      241 ggggatcaaa cagg
//
LOCUS       KHUT01009416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009416
VERSION     KHUT01009416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           86 g           56 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgtttgta ggcggttttc
       61 taagccagtg gtgaaagcct ggggctcaac cccagaattg ccattgggac tggaaagcta
      121 gagagtgtta gaggtaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gcttactggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009417
VERSION     KHUT01009417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtgagg
       61 caagtctgat gtgaaagctc gcagctcaac tgcgaaaagt cattggaaac tgccttgctg
      121 gaggcttgga ggggggactg gaatgctcgg tgtagcagtg aaatgcgtag atatcgagcg
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cgctgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01009418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009418
VERSION     KHUT01009418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggactta ctgggcgtaa agggtgcgta ggtggttgca
       61 taagtcagat gtgaaaggcc tgggcttaac ctaggaattg caattgaaac tgtgcgactg
      121 gagttgagta gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt tgcgaaggcg gctctctgga ctcaaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009419
VERSION     KHUT01009419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           63 c          102 g           36 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtgcgg
       61 cacgtggggt gtgaaagctg gccgcttaac ggtcagtcgc catcccatac ggccgtgctc
      121 gagccgtgca gagggcggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gccgcctggg cacgcgctga cgctgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009420
VERSION     KHUT01009420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtccggg gtgaaatcct cccgctcaac gggagaacgg cctcggatac tgacgatctc
      121 gagggggata ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaggcg gcgtgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009421
VERSION     KHUT01009421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          100 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggtgcgta ggcgggtcgc
       61 cgagtcgggg gtgaaatccc acggcccaac cgtggaaggg cctccgatac tggcgacctc
      121 gagggaggta ggggcgcgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009422
VERSION     KHUT01009422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccagg
       61 taagtctgat gtgaaatctc cgagcttaac tcggaaacgg catcggatac tatctggctt
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctaga cgattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009423
VERSION     KHUT01009423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           79 g           63 t
ORIGIN      
        1 tacgtagggt gctagagttg tccggattta ctgggtgtaa agggtgctca ggcgggtttg
       61 taagttagag gtgaaatccc aaagcttaac tttggtgctg cctttaatac tgcaagtctg
      121 gagtttgaga gagggtaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttacctggc tcaatactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009424             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009424
VERSION     KHUT01009424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          106 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgg
       61 cgcgcgtgcg gtgaaagccc ggggctcaac cccgggtctg ccgtgcggac ggccatggct
      121 agagcactgt agaggcaggt agaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaataccgg tggcgaaggc ggcctgctgg gcagttgctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009425             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009425
VERSION     KHUT01009425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agggcgagta ggtggttatg
       61 caagtcggat gtgaaaactc caggctcaac ctggagacgc cacccgatac tgcattgact
      121 agagttcggt aggggatcac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccgacactg acactgaatc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009426
VERSION     KHUT01009426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           76 g           63 t
ORIGIN      
        1 tacgaagggt ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtg gtgaaagcct gtagcttaac tacagaactg ccattgatac tgatgagctt
      121 gaatatactt gaggttggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctagctaaa gtatgattga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009427
VERSION     KHUT01009427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggattgt
       61 taagtcgggg gtgaaatccc agggcccaac cctggaactg ccttcgatac tgatgatctc
      121 gagtgtggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccattactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009428
VERSION     KHUT01009428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           85 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgagcgca ggcggaccgt
       61 taagtaggaa gtgaaaggtc ggagctcaac tccaacattg ctttctatac tggcggtctt
      121 gagtcacgga ggggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctttctgga cgatgactga cgctcaggct cgaaagccaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01009429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009429
VERSION     KHUT01009429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           81 g           58 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactgg
       61 ttagtctggg gtgaaatctc atcgcttaac ggtgaaactg ccttagatac tgccagtctt
      121 gaattaggga gaggttcgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcgaactggc ccttaattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01009430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009430
VERSION     KHUT01009430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           99 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcgagca ggcggtctct
       61 taagtttgac gtaaaatctc ctggcttaac tgggaggggt cgttgaagac tgggaggctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gcaccctggc attggcctga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009431
VERSION     KHUT01009431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggccatt
       61 caagtcaggg gtgaaatgcc agagcttaac tctggaactg cctttgaaac tgtttggctt
      121 gagactcgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009432
VERSION     KHUT01009432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           91 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgccg
       61 taagtttggc gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tgcggtgctt
      121 gagggttgga gaggaaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacacctgt ggtgtagacg gctttctgga caactcctga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009433
VERSION     KHUT01009433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           97 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctctg
       61 tgcgtggggt gtgaaagctc ttggcttaac taagagaggc catcccatac ggcagagctc
      121 gagcggtgca gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggg catcagctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009434
VERSION     KHUT01009434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          101 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtctgt
       61 cgcgtgtggc ctgacagttc ccggcttaac cgggaatggt ggtcgcaaac gggcagactt
      121 gagggcttca gagggacagg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccgaa ggcgaaggca ctgtcctggg gagtcactga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009435
VERSION     KHUT01009435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           98 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatg
       61 tatgtgtgat gtgaaagccc cgggcttaac ctgggaaggg catcgcgaac ggcatgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009436             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009436
VERSION     KHUT01009436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           87 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaatt gcttcgcata ctggctgact
      121 tgagggacgt agaggtgaat agaattcccg gtgtaacggt gacatgtgaa aatatcggga
      181 ggaataccaa tggcgaaggc agttcactgg acgtttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01009437             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009437
VERSION     KHUT01009437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcctgg
       61 taagtcgggt gtgaaatctc cgagctcaac tcggaggggc cactcgatac tgctatggct
      121 tgagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccga tagcgaaggc agcactctgg gccggtactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009438
VERSION     KHUT01009438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           91 g           45 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttgg
       61 caagtcaact gtgaaagccc agggcttaac cctggaagag cagctgaaac tgctgaattt
      121 gagtacagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009439             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009439
VERSION     KHUT01009439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           92 g           53 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggttgcg
       61 taagtctgat gtgaaatctt caccgctcaa cgggaagggg tcattggaaa ctacgcaact
      121 cgagtatggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacacctg tggcgaaagc ggcttgctgg gccattactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009440             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009440
VERSION     KHUT01009440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           33 c           76 g           64 t
ORIGIN      
        1 gacgagaggg gcgagcgtta tccggaataa ctgggcgtaa agggtccgta ggttgtagtt
       61 tgtatgcttt tagtaaaaga caaaaattta atttttgaaa tgctttaagg acagatctac
      121 tagagtttag cagaggataa tagaattcca tgtggagggg tggaatccta agatatatgg
      181 aggaatgcca gtagcgaaag cgattatctg ggctattact gacactgagg gacgaaagcg
      241 taggtagcaa acagg
//
LOCUS       KHUT01009441             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009441
VERSION     KHUT01009441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           76 g           62 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtggaa
       61 taagttattg atctaagtcg agctcaactt gatggaagtt gataatactg ctctactgga
      121 ggacaggaga ggttaacgga attctcataa gtaggggtga aatccattga tctatggagg
      181 accaccaaaa aaggcgaagg cagttaacta ttctgttcct gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01009442             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009442
VERSION     KHUT01009442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          103 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggtcagg
       61 cgcgttttgt gtgacagcgg agggcttcac tctccgagca tacgaaagac ggcgtgactt
      121 gagggctcga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggc gagtatccct gacgctgagg tgcgaaagcc
      241 aggggagcga acggg
//
LOCUS       KHUT01009443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009443
VERSION     KHUT01009443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           86 g           50 t
ORIGIN      
        1 gacgagcctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcagcttaac tgaggaactg cgcccaaaac taccgtgctt
      121 gagggagata gaggaatgcg gaacttacgg tggagcggtg aaatgcgttg atatcgtagg
      181 gaacaccggt ggcgaaagcg gcattctgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009444
VERSION     KHUT01009444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           82 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggccgg
       61 taagtcagtg gtgaaatcct ggggcttaac cccagaactg ccgttgatac tatcggtctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag agatgacata
      181 gaacaccaat tgcgaaggca gctcactacg caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009445
VERSION     KHUT01009445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttacg
       61 taagtgagat gtgaaatccc cgggcttaac ctgggacgtg cgtcttatac tgcgtgactg
      121 gagtgtagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctaacactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009446
VERSION     KHUT01009446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgac
       61 caagtcaggg gtgaaatcct tcagcttaac tggagaacag cctctgatac tggccgtctg
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009447             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009447
VERSION     KHUT01009447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           77 g           63 t
ORIGIN      
        1 tacagagggt gcaagcgtta tccggattta ctgggcgtaa agcgtttcgt aggcggttaa
       61 gatagtcata cttcaaagcc cagggctcaa ccctggaacg gggtatgata cttctttgac
      121 tagaaagatt tcggggtttt cagaactctt ggtgtaggag tgaaatccgt tgatatcaag
      181 aggaattcca agggcgtagg cagaaaacta gggattcttt gacgctgatg aacgacagct
      241 agggtagcga aaggg
//
LOCUS       KHUT01009448             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009448
VERSION     KHUT01009448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           88 g           46 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcccgg
       61 aaagtttgat gtgaaagctc ggagctcaac tccgaaatgg cattgaatac tcccgggcta
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgaaaaccct gacactgagg cacgaaagct
      241 aggggagcaa acagg
//
LOCUS       KHUT01009449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009449
VERSION     KHUT01009449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           75 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaacga
       61 taagtcagtg gtgaaagccc tcagcttaac tgaggaactg ccattgatac tgtcgatctt
      121 gagtacattt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01009450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009450
VERSION     KHUT01009450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           91 g           46 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccgtt
       61 taggtagggt gtgaaatccc ccggcccaac cggggaatgg cgctctaaac cgtacggctc
      121 gagtgaggca ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaaccccggt ggcgaaagcg acacactggg ccttaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009451
VERSION     KHUT01009451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgtc
       61 taagtgcctt gtgaaatccc acggctcacc cgtggaactg ctgggcatac tggatgtctt
      121 gagccactta ggggtcgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggcgactggg agtgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009452
VERSION     KHUT01009452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatc
       61 caagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac ggagtaactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgcg
      241 gggagcgaac agg
//
LOCUS       KHUT01009453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009453
VERSION     KHUT01009453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           89 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggatcag
       61 taagtcaggt gtgaaatctc agagctcaac tctgaaactg cgcctgaaac tactagtcta
      121 gaatgtcgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggg ggcgaaggcg cctacctgga cgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009454
VERSION     KHUT01009454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaat
       61 taagttagat gtgaaagccc tgggcttaac ctaggaattg catctaagac tggttagctt
      121 gagtgcggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009455
VERSION     KHUT01009455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           98 g           47 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agaacgcgta ggcggttcgt
       61 taagtttggc gtaaaatctc ctggctcaac tgggagggcg caccgaagac tggcggactg
      121 gagggcatca gaggaaagcg gaactcccgg tgtagtggtg aaatgcgtag agatcgggaa
      181 gaacacctgt ggcgaaggcg gctttctggg gtgtacctga cgctgaggcg tgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009456
VERSION     KHUT01009456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggctaga
       61 taagtcagat gtgaaatccc tgggcttaac ctgggaaagt catttgagac tgtgtggcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gctttctgga cttatactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009457
VERSION     KHUT01009457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggctatg
       61 taagtcagag gtgaaagccc agggctcaac cttggaattg cctttgagac tgcgtagctt
      121 gaatcgcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgcgtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009458
VERSION     KHUT01009458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           87 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgccg
       61 taagtcagat gtgaaagccc ggggctcaac cccggaattg catccgatac tgcggtgcta
      121 gagaactgga gaggagtcta gaattttcgg tgtagcagtg aaatgcgtag atatcgaaag
      181 gaataccaaa ggcgaaggca ggactctgga cagtttctga cgctgaggca cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009459             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009459
VERSION     KHUT01009459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           77 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttattc ggtgaaaaac cagggctcaa ccctgtgtaa gccagtaata ctatttaact
      121 agagggccgg agaggttaac ggaattctat gtgtagcggt aaaatgctca aatatataga
      181 ggaacaccaa tcggcgaagg cagttaacta ttcggttcct gacgttgagg cacgaaagcg
      241 tggggagcaa gacgg
//
LOCUS       KHUT01009460             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009460
VERSION     KHUT01009460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           83 g           55 t
ORIGIN      
        1 tacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtcgga
       61 taagttagca gtgggaaatc agggctcaac cctgcggaag ttgctaatac tgtctgactg
      121 gaaggcagga gaagttaacg gaactccctg tggagcggta aaatgtgtag atatagggaa
      181 gaacaccaag taggcgaagg cagttaacta ttctgttctt gacattgagg cacgaaagcg
      241 tggggagcaa aaagg
//
LOCUS       KHUT01009461             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009461
VERSION     KHUT01009461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctga
       61 tttgtctgtc ggtgaaagcc tggggcttaa ccccagaagt gccacagaaa cggtcagact
      121 tagagtactg gagaggtgag cggaattccc ggtgtagcgg tgaaatgcgt agagatcggg
      181 aggaacacca gcggcgaagg cggctcactg gacagtcact gacactcaac tacgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01009462             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009462
VERSION     KHUT01009462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           81 g           63 t
ORIGIN      
        1 tacgagaggt gcaagcgttg tccggattta ttgggcgtaa agcgtttcta aaggcttttt
       61 tgtaagttat tcttcaaaga ccgaggctca acctcgggaa ggggggtaat actgcaagaa
      121 tcgagatatt tcggggttac tagaactatc ggtgtagggg tgaaatccgt tgatatcgat
      181 aggaattcca agggcgaagg caggtaacta ggaatgttct gacgctgatg aacgaaagct
      241 aggggagcga aaggg
//
LOCUS       KHUT01009463             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009463
VERSION     KHUT01009463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           41 c           74 g           58 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta ggtggtagaa
       61 taagttacta gttaaagcta ggctcaactt gacggaagct agtaaaactg ttctgctaga
      121 ggacaaaaga ggttgacgga actcacatga gtaagggtga aatctattga tccatggagg
      181 accaccaaaa aaggcgaagg cagttaacca ttttgttcct gacactgagg catgaaagcg
      241 tgggtagcaa actgg
//
LOCUS       KHUT01009464             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009464
VERSION     KHUT01009464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           43 c           77 g           53 t
ORIGIN      
        1 tacaggggat gcaagcgtta tccggaatga ttgggcgtaa agcgtttgaa caggtggttt
       61 agaaagtcga ttgttaaaac ccaaggctta accttggacc ggcaatggaa acttctagac
      121 tagagtgtgg tagaggtaga tggaattacc ggtgtaacgg tgaaatgtgc agatatcggt
      181 aagaaccccg acggcgaaag caatctactg ggccaaaact gacacttaag aacgaaagct
      241 aggggagcga atagg
//
LOCUS       KHUT01009465             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009465
VERSION     KHUT01009465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 caagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac tgctatggct
      121 tgagtttggt aggggaagac ggaattcctg gtgtagcggt gaaatgcgca gatatcagag
      181 aggaacaccg atggcgaagg cagtcttctg ggccaaaact gacgctgagg agcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01009466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009466
VERSION     KHUT01009466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           87 g           47 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggtggttcgg
       61 taagtctgtt gtgaaagctc ccggctcaac tgggagaggc caatggaaac tgccggactt
      121 gaatcgagga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaagca ggtgactgga ctcgtattga cactcatcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009467
VERSION     KHUT01009467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           95 g           51 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtcgcc
       61 tatgtcgggt gtgaaagccc acggcttaac cgtggaacgg cattcgaaac tgggtgactt
      121 gagtgcagga ggggagagcg gaattcttgg tgtagcggtg aaatgcgttg atatcaagaa
      181 gaacaccggt ggcgaaggcg gctctctgga atgctactga cactgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01009468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009468
VERSION     KHUT01009468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcggcctga
       61 cccgtctgat gtgacagctc gcggctcaac tgcgagaggc cattggaaac agtcaggctt
      121 gaatcaggcc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gctggctagg ccctgattga cgctgaggga cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009469
VERSION     KHUT01009469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           80 g           53 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggactcg
       61 caagtcagaa gtgaaatccc tgggcttaac ccaggaattg cttttgaaac tgcaagtcta
      121 gaatgttgga gaggttagca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccaat ggcgtaggca gctaactgga caactattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009470
VERSION     KHUT01009470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           84 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcttt
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgaaggactt
      121 gagttcagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgtagaca gcttactagg ctgaaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009471
VERSION     KHUT01009471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcattg
       61 caagtcgggt gtgaaatccc tcagctcaac tgaggaagtg cgcccgaaac tgcagtgctt
      121 gagtaccgga gagggtctcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gggacctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009472
VERSION     KHUT01009472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           84 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaaga
       61 taagtcagat gtgaaagccc ggggctcaac cccggaactg catttgaaac tatcattcta
      121 gagggtaggc ggagaaaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009473
VERSION     KHUT01009473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           61 c          111 g           36 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agggcacgca ggcggcgcga
       61 tgagcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac tgtcgcgctt
      121 gagggcagca gggggtggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccacctggg ctgcacctga cgctgaggtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009474
VERSION     KHUT01009474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 tacagaggtt gcaagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggctata
       61 caagtcggtt gtgaaatccc tgggctcaac ctgggaactg cttccgagac tgtatggcta
      121 gagatcggta gagggcggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccaat ggcgaaggca gccgcctggg cctgatctga cactgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009475
VERSION     KHUT01009475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 cacggagggg gcgagcgtta ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgg
       61 caagtcaaag gtgaaagccc tcggcctaac cgaggaactg cctttgaaac tgcctcgctt
      121 gaggccggaa gggggtagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gctacctggg ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009476             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009476
VERSION     KHUT01009476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           78 g           61 t
ORIGIN      
        1 tacgtaggtc acaagcgtta tccggaatta ctgggcgtga agagtctcgt aggcggtgat
       61 ataagtggta ggttaaagac ccttgcttaa cttggggaat gcttatcaaa ctgtattact
      121 agagttagtt cggggatgct ggaattctta gtggaggggt gaaatccgtt gatattaaga
      181 agaacaccaa aggcgaaggc aggcatctag gacttaactg acgctgatga acgaaagcta
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01009477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009477
VERSION     KHUT01009477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctcg
       61 caagtcgggc gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tgcgaagctg
      121 gagtcctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg accttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009478
VERSION     KHUT01009478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 tacagagggt gctaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcccgt
       61 caagtgcggt gtgaaatccc acggcttacc cgtggaaatg cgctgcagac tgacgggctc
      121 gaggacggta ggggagagcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccgtttctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009479
VERSION     KHUT01009479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           90 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaat
       61 tgagtgagtt gtgaaatccc tgggcttaac ctaggaattg caacttatac tggttgacta
      121 gagtgcagta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actatctgga ctgagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009480
VERSION     KHUT01009480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          100 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctggg
       61 taagtagggg gtgaaatctc acggcttaac cgtgaggctg ccttctagac tgcctggctt
      121 gagcatagta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gctcgctggg ctattgctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009481
VERSION     KHUT01009481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 gacagaggtt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggctagg
       61 tatgtctgac gtgaaatccc tcggcttaac cggggaatgg catcagaaac tacttggctt
      121 gagtgtgaga gaggatagcg gaattcccag agtagaggtg aaattcgtag atactgggag
      181 gaacacctga ggcgaaggcg gctatctggt tcacaactga cgctgagacg caaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009482
VERSION     KHUT01009482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggattca ctgggcttaa agggtgcgta ggcggccgtt
       61 taagtagggt gtgaaagccc ccggctcaac cggggaatgg cgccctaaac tggacggctg
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcgactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009483
VERSION     KHUT01009483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggattca ttgggcttaa agcgtacgta ggcggacgtg
       61 caggcgtcgt gtgaaagccc tcggctcaac cggggaattg cacggcgaac cgcacgtctt
      121 gagatcggta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggccgctggg ccgattctga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009484
VERSION     KHUT01009484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           86 g           46 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agtgtctgta ggtggctttt
       61 caagtctgtc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttctgg tggagcggtg aaatgcattg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01009485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009485
VERSION     KHUT01009485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggcggctggt
       61 tgtgtcgagt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgaccggctt
      121 gagtacggga gaggagggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg accctctgga ccgatactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009486
VERSION     KHUT01009486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           85 g           55 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggacctc
       61 caagtcagga gtgaaatccc agggcttaac cctggaattg ctcttgaaac tggtggtctt
      121 gaatcctgga ggggttggga gaattcctag tgtagaggtg aaattcgcag atattaggag
      181 gaataccagt ggcgtaggcg cccaactgga caggtattga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009487
VERSION     KHUT01009487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 aacgtaggac acaagcgtta tccggaatta ctgggcgtaa agggcgtgca ggcggttgcg
       61 taagttaggc gtgaaagact tcggctaaac cgggggaggt cgtctgatac tgcgtaactc
      121 gagtgtggta gaggcgtgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgaaggcg gcacgctggg ccattactga cgctaagacg cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009488
VERSION     KHUT01009488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          103 g           39 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgtgcgca ggcggtgcga
       61 taagtggggt gtgaaatctc ccggcttaac cgggagggag cgcctcagac tgtcggacta
      121 gagtgggaga gagggacgtg gaattccggg tggagcggtg aaatgtgtag agatccggag
      181 gaacaccaga ggcgcaagcg gcgtcctggg tcccaactga cgctgaggca cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009489
VERSION     KHUT01009489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 gacagaggag gcaagcgttg ttcggaatca ttgggcttaa agcgcgtgta ggcggcttcg
       61 taagtgtctt gtgaaatccc gtggctcaac cacggaactg ctgggcatac tgcgaggctt
      121 gaggttggta gaggtgcctg gaactcttgg tggagcggtg aaatgtgtag atatcaagag
      181 gaacgccgga ggcgaaagcg gggcactggg ccaaatctga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009490
VERSION     KHUT01009490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          103 g           39 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatta ctgggcgtaa agcgctcgta ggcggtcgca
       61 tacggcggtg gtgaaagctc ccggctcaac tgggagaggc ccatcgcaac ggtgtgactc
      121 gagggtggta gaggcagacg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gtctgctggg ccattcctga cgctgaggag cgaaagcgag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009491
VERSION     KHUT01009491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttcgc
       61 taagtcggac gtgaaagccc tcggctcaac cgaggaagtg catccgaaac tgacgagcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009492
VERSION     KHUT01009492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           85 g           59 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcttcctgt
       61 taagtcatct tttaaagacc gaggctcaac ttcggaaagg gggatgatac tggcaggatt
      121 gaaatatttc ggggctactg gaacttacgg tgtaggagtg aaatccgttg atatcgtaag
      181 gaacaccaag ggcgaaggcg ggtagctagg aattatttga cgctgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01009493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009493
VERSION     KHUT01009493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           84 g           52 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatca ctgggcgtaa agagtgcgta ggcggttatg
       61 aaagttagga gtgaaagccc agggcttaac cttggaattg ctcttaaaac tacatgacta
      121 gaattcggga gaggatagcg gaattgtcag tgtagcagtg aaatgcgtag atattgacag
      181 gaacaccagt ggcgtaagcg gctatctgga ccgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009494
VERSION     KHUT01009494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           96 g           41 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgta ggcggtttgg
       61 taagtcgggt gtgaaagccc caggcttaac ctgggaatgg cgcccgaaac tgcctcactg
      121 gagggacgga gaggtgcacg gaattcccgg tgtaggggtg aaatccatag atatcgggag
      181 gaacaccaat ggcgaaggca gtgcactgga cgtcacctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009495
VERSION     KHUT01009495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggatcgg
       61 caagtcagaa gtgaaatccc tgggctcaac ccaggaattg cttttgagac tgtcggtctt
      121 gagtacagga gaggtgagtg gaattcccag tgtagaggta aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009496
VERSION     KHUT01009496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 caagtccggg gtgaaatcct ccagctcaac tggagaagtg ccttggatac tgccggtctc
      121 gagggaggca ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactggt cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009497
VERSION     KHUT01009497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 tacgtatggg gcgagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggattgt
       61 taagcgagat gtgaaaactg ggggctcaac ccgcagcctg catctcgaac tggcagtctt
      121 gagtctggta gaggaaagtg gaattcccag tgtagcggtg aaatgcgcag atattgggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009498
VERSION     KHUT01009498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           98 g           40 t
ORIGIN      
        1 gacgaaggtg gcgagcgttg ttcggaatca ctgggcataa agggcgcgta ggcggtccct
       61 taagtcggat gtgaaagccc ccggcccaac cggggaactg cgtccgatac tgagggactt
      121 gagggtcgga gaggtgcatg gaattcctgg tggagcggtg aaatgcgttg atatcaggag
      181 gaacaccgga ggcgaaggcg atgcactggc cgatctctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009499
VERSION     KHUT01009499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           87 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaaa
       61 taagttaggt gtgaaattcc tgagctcaac ttggggactg cgcctgatac tgtttggcta
      121 gagtactgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009500
VERSION     KHUT01009500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           49 t
ORIGIN      
        1 tacaggggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggctggcccg
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaattg cagggcatac tgcgggtctt
      121 gaggaaggta gaggtcgacg gaacgatagg tggagcggtg gaatgcatag agatctatcg
      181 gaacgccaaa agtgaagaca gtcgactggg cctttcctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01009501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009501
VERSION     KHUT01009501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgtgca ggcggggtgg
       61 taagtggtgt atgaaatctc ctggctcaac caggagcggt tatgccagac tgccactctt
      121 gagtgcgaga gaggagcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgctctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009502
VERSION     KHUT01009502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 gacgaaccgt gctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtttgc
       61 aaagtcaagg gtgaaatctt tcggcccaac cggaaaaggg cctttgatac tggcaaacta
      121 gagcgaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009503
VERSION     KHUT01009503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacggggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtctct
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tgaaaggctt
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009504
VERSION     KHUT01009504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcga
       61 ttagtcaggg gtgaaagccc agagctcaac tctggaactg cctttgatac tgtcgatctg
      121 gagcagagta gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactgga ctcttgctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009505
VERSION     KHUT01009505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacagagatg gcaagcgttg ttcggaatca ctgggcataa agggcacgta ggcgggttgg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaactg cgcctgaaac tgtcgatctt
      121 gagggcagga ggggagagtg gaactcttgg tggagcggtg aaatgcgtag ataccaagag
      181 gaacgccggt ggcgaaagcg actctctggt ctgctactga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009506
VERSION     KHUT01009506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattca ttgggcgtaa agcgcgtgta ggcggcttta
       61 caggtccgct gtgaaaactc gaggctcaac ctcgagacgt cggcggaaac cgtatggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009507
VERSION     KHUT01009507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgca ggcggctgag
       61 taagacagat gtgaaatccc cgggcttaac ctgggaaccg catatgtgac tgcttggcta
      121 gagtgcgtca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggaa
      181 gaacaccgat ggcgaaggca gcctcctggg acgcaactga cgctcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009508             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009508
VERSION     KHUT01009508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           59 c           83 g           48 t
ORIGIN      
        1 cccaggaccc acaagtggca accgctttta ttgggcctaa agcgtccgta gccggatctg
       61 taaatcttct gtgaaatcct tcggcttaac cggagggcgc gcaggagaca ctgcatttct
      121 agggaccggg aggcgtaagg ggtatttcgg ggggagcggt aaaatgttat aatcctcgaa
      181 ggaccacctg tggcgaaggc gccttacgag aacggatccg acggtgagtg acgaaagcta
      241 ggggagcaaa acgg
//
LOCUS       KHUT01009509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009509
VERSION     KHUT01009509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           81 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggagatg
       61 taagtcagac gtgaaatccc ggggctcaac ctcggaacag catttgaaac tacatctctc
      121 gagggtagac ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagt ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01009510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009510
VERSION     KHUT01009510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 aacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggtggcccgt
       61 gaagtcgaga gtgaaaaccg agggcttaac cttcggcctg ctttcgaaac cagcgggctt
      121 gagtgcagta gagggaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg gcttcctggc ctgcaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009511
VERSION     KHUT01009511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gacggaatca ttgggcttaa agcgtacgta ggcgggcgtg
       61 caggcgtcgt gtgaaagccc tcggctcaac cggggaattg cacggcgaac cgcacgtctt
      121 gagacaagta ggggcggccg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccaat ggtgaagaca ggccgctggg cttgttctga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009512
VERSION     KHUT01009512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgttg cgcggaatca ctgggcttaa agggtgcgta ggccgatatg
       61 caagtgcttt gtgaaatccc agagcttaac tctggaactg cttggcagac tgcatgtctt
      121 gaggtcggta ggggttgctg gaacggttgg tggagcggtg aaatgcgtag atatcaaccg
      181 gaacgccaaa ggtgaagaca agcaactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009513
VERSION     KHUT01009513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcataa agggcgcgta ggcggtgaga
       61 tgtgagagat gtgaaagccc cgggcttaac ctgggaaggg catttctaac tatttgacta
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009514
VERSION     KHUT01009514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtccgat gttaaagacc ggggctcaac cccgacatgg cgttggatac tggcaaacta
      121 gacgactgga gaggaaagta gaattaccag tgtagcggtg aaatgcgtag atactggtag
      181 gaatacccat tgcgaaggca gctttctgga cagtccgtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01009515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009515
VERSION     KHUT01009515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctatg
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tgcgtagctc
      121 gagtcctgga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccacctgga cgggtactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009516
VERSION     KHUT01009516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggattta ttgggcgtaa agggtgtgta ggcggtttgc
       61 taagtcaact gttaaatttc tcggcccaac cgagagtcag cagtagatac tggcatccta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009517
VERSION     KHUT01009517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggacttg
       61 taagtcaggt gtgaaagccc tgggctcaac ccaggaactg catctgaaac tgctcgtctt
      121 gagtgtcgga gagggaagcg gaattccggg tgtagaggtg aaattcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009518
VERSION     KHUT01009518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           37 c           86 g           59 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtttgta ggcggttgtt
       61 taagctagtg gtgaaagccc agggcttaac cctggaattg ccattagaac tggacagcta
      121 gagagtgtta gaggaaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gctttctggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009519
VERSION     KHUT01009519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           43 c           94 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggtcctg
       61 taagtatggt gtgaaatctc ccggcttaac tgggagggtg cgctatagac tgcgggacta
      121 gagggtggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtaaacg gctttctgga ccatttctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009520
VERSION     KHUT01009520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcggat gtgaaatccc caggcttaac ctgggaactg catccgatac tggatggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgagattga cgctgatgag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009521
VERSION     KHUT01009521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgtacggt acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggctcag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaatcg cgctcaaaac tactgggctt
      121 gagggaggta gaggtgggcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaagcg gcccactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009522
VERSION     KHUT01009522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 gacgaacctg acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaactg cgcccaaaac tactgtgctt
      121 gagtgagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcaactga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009523
VERSION     KHUT01009523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c          101 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttga agggcgcgta ggcgggcgac
       61 caagtccggg gtgaaagccc acggcccaac cgtggaaggg ccccggatac tggccgcctc
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009524
VERSION     KHUT01009524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           38 c           82 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaataa ttgggcgtaa agggtgcgta ggtggttatt
       61 tatgtcttat gtgaaagtca atggctcaac cattgaatgc catgagaaac tggataacta
      121 gagtactgta gagggtagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcagt ggcgaaggcg actacctgga cagatactga cactgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009525
VERSION     KHUT01009525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           39 c           96 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 tgagatagct gtgaaatacc tgggcttaac ctgggggagt cagctgttac tgttagactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacactggt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009526
VERSION     KHUT01009526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c          102 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggcggctgtt
       61 taagtgtgct gtgaaatgtc ccggctcaac cgggaaggtg cggcgcagac tggacggctg
      121 gagggtgaca gaggcaggta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgtaagcg gcctgctggg tcaatcctga cgctgagacg cgaaggcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009527
VERSION     KHUT01009527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggctata
       61 taagttatgt gtgaaatctc tgggcttaac ctgggaactg cccataatac tgtatagcta
      121 gagtagagta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctttctgga ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009528
VERSION     KHUT01009528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 tacggaggtg gcgagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggggcgg
       61 caagtgtcgg gtgaaagccc tcggctcaac cgaggaacgg cctggcaaac tgccgtcctt
      121 gaggtagaca gaggtcactg gaacgacggg tggagcggtg aaatgcgtag atatccgttg
      181 gaacgccggc ggcgaaagcg agtgactggg tctgtcctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009529
VERSION     KHUT01009529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtccta
       61 tttgtctggg gtgaaatcct tgagcttaac tcaagaagtg ccccggaaac ggtaggactc
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtcactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009530
VERSION     KHUT01009530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           71 c          103 g           29 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgcg
       61 caggtcccgg gtgaaagccc ccggcccaac cggggaagtg cccgggatac cgcgcggctc
      121 gagcgaggca ggggcggggg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg ccccgctggg cctctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009531
VERSION     KHUT01009531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggtttga
       61 caagtcctat gtgaaatctt ctggcttaac caggagcggt cgtgggaaac tgttgagctg
      121 gaggcgatga gagggatgca gaattccagg tgtagtggtg gaatgcgtag atatctggag
      181 gaataccagt ggcgaaagcg gcatcctggc atcggcctga cgcacaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009532
VERSION     KHUT01009532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttagt
       61 taagttggat gtgaaagccc cgggctcaac ctgggaattg cattcaaaac tggctaacta
      121 gaatacggga gaggggggta gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaataccagt ggcgaaggcg gccccctggc ccgatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009533
VERSION     KHUT01009533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           83 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgtcta ggtggatctg
       61 taagtcaggt gttaaagctc ccggctcaac cgggaaaagt cgcttgatac tgcagatctt
      121 gagtttcggg gaggtgagtg gaattcccag tgtagaggtg aaattcgtag agattgggag
      181 gaacaccggt ggcgaaagcg actcactaac cgaacactga cactcaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009534
VERSION     KHUT01009534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcggaccgg
       61 caagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaaaac tgccggactt
      121 gagtatggga gagggaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctggc ccaaaactga cgctcagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009535
VERSION     KHUT01009535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgt
       61 caagtccggg gtgaaatcct ccagcctaac tggagaagcg cctcggagac tggcggcctc
      121 gaggagggta ggggcaagcg gaactatggg tggagcggtg aaatgcattg atatccatag
      181 gaactccggt ggcgaaggcg gcttgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009536
VERSION     KHUT01009536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           77 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtgcgca ggcggaattt
       61 taagtcagtg gtgaaatccc ttcgcttaac gaaggaactg ccattgatac tgaaattctt
      121 gaatttggtt ggcgtgggcg gaatgtgcca tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat cgcgaaggca gctcacgaaa ccattattga cgctcaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009537
VERSION     KHUT01009537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcatgca ggcggtgctg
       61 taagttgggt gtgaaagctc ctggctcaac tgggagaggt cgctcaagac tgcagcactt
      121 gagaatggta ggggaacgtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcgttctgga ccattactga cgctcagatg cgacagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009538
VERSION     KHUT01009538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcaa
       61 taagtctctt gtgaaagctc ccggcttaac tgggagaggt caagggaaac tgttgggcta
      121 gagtgtggca gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccgtcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009539
VERSION     KHUT01009539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           81 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggggcat
       61 taagtcagag gtgaaatctt gcggctcaac cgtaaaattg cctttgaaac tggtatcctt
      121 gagttcggtt gaggtggatg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gtccactaag ccgacactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009540
VERSION     KHUT01009540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcgacg
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tgcattgctt
      121 gagtaccgga gaggatggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccatctgga cggatactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009541
VERSION     KHUT01009541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c          103 g           39 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcggcgggg
       61 taagtggctt gtgaaagccc tcggctaaac cgggggaggt caggtcagac tgccaagcta
      121 gagggaggta gaggagcgtg gaattcgggg tggagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggtgcaaacg gcgctctggg cctttactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009542
VERSION     KHUT01009542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttata
       61 tgtgtgggat gtgaaagccc tgggcttaac ctaggaagcg cataccaaac tgtataactg
      121 gagtatatga gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgccaat ggcgaaggca gctacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009543
VERSION     KHUT01009543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           84 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctaat
       61 taagtcagtg gtgaaagccc gcagctcaac tgtggaactg ccattgatac tggttagctt
      121 gagtttggtt gaggtaagcg gaatggatag tgtagcggtg aaatgcatag atattatcca
      181 gaacaccgat agcgaaggca gcttactggg ccgatactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009544
VERSION     KHUT01009544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           93 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcttc
       61 taagtcggat gtgaaagccc caggctcaac ctgggaagtg catacgaaac tggaaggctg
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009545
VERSION     KHUT01009545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggctgttcgt
       61 taagtcttgg gtgaaatctt tcggcttaac cggaaaattg cccgggatac tggcgagctc
      121 gagggtggta ggggcgatcg gaactgccag tggagcggtg aaatgcgttg atattggcag
      181 gaacgccggt ggcgaaagcg gatcgctgga tcactcctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009546
VERSION     KHUT01009546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggtctg
       61 taagttgggg gtgaaatccc cgggctcaac ccgggaactg ccttcaagac tgcagatctt
      121 gagatcagga gaggcgagcg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009547
VERSION     KHUT01009547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta ctcggattta ctaggcgtaa agcgcttgta ggtggttgga
       61 taagtctgta gtgaaatttc ccggctcaac cgggaaaggt ctatagaaac tatccggctt
      121 gagtgtggaa gggggtgacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaagca gtcacctggt ccaatactga cactgaggag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009548
VERSION     KHUT01009548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           83 g           50 t
ORIGIN      
        1 gacggaaggt gcaagcatta ctcggaatca ctgggcgtaa agcgtccgca ggtgttcttg
       61 tgtgtctggt gtaaaagcac gaagctcaac tccgtgtacg tgccggaaac tgcaagaatt
      121 gagtcactca gaggcatctg gaatgtcatg tgtaggggta aaatccgttg atccatgata
      181 gaacgccaaa agcgaaggca ggatgctggg ggtgtactga cactcaggga cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009549
VERSION     KHUT01009549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 gacggagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggccaac
       61 taagtcgggt gtgaaagccc ccggctcaac cgaggaattg ctcccgatac tggttggctc
      121 gaggatggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga tcatcactga cgctgaggtg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009550
VERSION     KHUT01009550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ctgggtataa agggtgcgca ggcggaactg
       61 tgcgtcggag gttaaagacc cgggctcaac ccgggaagtg ccttcgatac ggcaggtctt
      121 gagtgtgaga gaggatagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccagt ggcgaaggcg gctatctggc tcataactga cgcttaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009551
VERSION     KHUT01009551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           89 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttatct gtgaaattcc tgggcttaac ctgggacggt cagataatac tgttgaactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009552
VERSION     KHUT01009552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa aggggaggta ggtggtctcg
       61 tttgtccgag gtgaaatccc taggcttaac ctaggacgtg cctcggaaac ggcgagacta
      121 gagtactgga gaggctagcg gaatacccgg tgtagcggtg aaatgcgtag agatcgggtg
      181 gaacaccaga ggcgaaggcg gctagctgga cagttactga cactaaactc cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009553             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009553
VERSION     KHUT01009553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           49 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 taagtcgggt gtgaaaactc agggctcaac tctgagatgc cacccgatac tgctatgact
      121 agagtccggt agaggagcat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccaa ttgcgaaggc agtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01009554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009554
VERSION     KHUT01009554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           84 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccac
       61 taagtcagtt gtgaaatacg gcagcttaac tgtcgaggtg caattgatac tggtaggcta
      121 gagtactgat gaggtatgcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcatactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009555
VERSION     KHUT01009555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           39 c           93 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtgggataa
       61 taagttagct gtgaaattcc tgggcttaac ctagggaggt cagctaagac tgttaatctg
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcggt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009556             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009556
VERSION     KHUT01009556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 caagtcgatg gtgaaagccc ggagctcaac tccggaatgg ccattgaaac tgcttggctt
      121 cgagtgcggg agaggtgagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggctcactgg cccgttactg acgctcaggc gcgacagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01009557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009557
VERSION     KHUT01009557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           53 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaataa ctgggcgtaa agggagcgca ggcggcttgg
       61 taagtctaaa gtgaaattcc agggctcaac cctggacgtg ctttggatac tgcctggctt
      121 gagtacgtga gaggagattg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gatctctggc acgttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009558             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009558
VERSION     KHUT01009558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggctgtttgg
       61 taagtcggat gtgaaaactc cgggctcaac tcggagacgc cattcgatac tgccatgact
      121 agagtccggt aggggggtgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccaa tggcgaaggc agcaccctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009559
VERSION     KHUT01009559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           43 c           93 g           54 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttcgt
       61 taagtctgat gttaaagatc ggggctcaac cccgggagtg cattggatac tggcgagctg
      121 gagtgcggta gaggtaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgtaggcg acttactggg ccgtaactga cgctgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01009560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009560
VERSION     KHUT01009560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 caagttcggg gtgaaatctt ccggcttaac cggaaaatag ccttggatac tggtcgtctc
      121 gagcgaggta ggggcagttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aactgctggg cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009561
VERSION     KHUT01009561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           85 g           56 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggctgtaatg
       61 caagtcaggg gtgaaatgcc gaagcttaac ttcggaactg cctttgaaac tgcattgcta
      121 gaatatggga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctggc ccattattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009562
VERSION     KHUT01009562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggataag
       61 aaagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tatttgtcta
      121 gagtacgaga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacgtcaat ggcgaaggca gccacctggc tcgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009563
VERSION     KHUT01009563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           89 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctggtatc
       61 caagtcaggg gtgaaatcgt tcggcccaac cggacaaggg cccctgatac tgggtatctc
      121 gagggaggta ggggtcgatg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009564
VERSION     KHUT01009564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           79 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggtgggtgtt
       61 taagtgagtg gtgaaatctt ccggcttaac cggaaaattg ccattcatac tggatacctt
      121 gagtatagtt gaggcgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaag ctattactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009565
VERSION     KHUT01009565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           51 c          101 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggttgg
       61 caagtctgct gtgaaagccc ggagctcaac tccggagggt cggtggatac tgccagtctt
      121 gaagtggcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gctcacttga cgctgatgcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009566
VERSION     KHUT01009566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           83 g           55 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtatgt
       61 taagtctgac attaaagact ggagcttaac tccaggactg tatcggaaac tggcaaacta
      121 gaggacaata gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg gacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009567
VERSION     KHUT01009567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggtccat
       61 caagtcggtt gtgaaagccc ttggctcaac caaggaagtg cgtccgagac tggtgggcta
      121 gagttctgaa gagggtggcg gaatgcgcag tgtagaggtg aaattcgtag atattgcgta
      181 gaacaccagt ggcgaaggcg gccacctggg aagcgactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009568
VERSION     KHUT01009568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcgg
       61 taagtcagac gtgaaagccc tgggctcaac ccaggaattg cgcttgaaac tgccggtctt
      121 gagtggtgga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gcttcctgga caccgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009569
VERSION     KHUT01009569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtctat
       61 taagtctggt gttaaaaacc gaagctcaac ttcgacacgg catcggaaac tggtaggcta
      121 gagtgtggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaagcg ccttgctggg ccattactga cgctgaggaa cgaaagctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01009570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009570
VERSION     KHUT01009570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgca ggcggctttc
       61 caagtcagag gtgaaatgcc ggggctcaac cccggaactg cctttgaaac tggatcgcta
      121 gagtctcgga ggggttaggg gaatttctaa tgtagcggtg aaatgcgtag atattagaag
      181 gaacaccagt ggcgaaggcg cctaactgga cgagtactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009571
VERSION     KHUT01009571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           88 g           56 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttttg
       61 taagtcagga gtgaaatccc ggagcttaac ttcggaattg cttttgaaac tacaaggcta
      121 gagtgagtta gaggatggcg gaattcctag tgtagtggtg aaatacgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg actcaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009572
VERSION     KHUT01009572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggtggtccag
       61 taagtccgct gtaaaagtca agggctcaac ccttgaaagc cggtggatac tgttggacta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggt acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009573
VERSION     KHUT01009573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           79 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggtttc
       61 caagtcagtg gtgaaagctc cgagcttaac tcggaaattg ccattgatac tggaagtctt
      121 gaattcagtt gaggtcggcg gaatgtgtcg tgtagtagtg aaatacttag atatgacaca
      181 gaacaccaat tgcgaaggca gctggctaac ctgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009574
VERSION     KHUT01009574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           92 g           44 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtcctc
       61 taagtcagat gtgaaatcct acggctcaac cgtggaactg catctgatac tggaggactt
      121 gaggacggta gaggtcggtg gaattcccgg tgtaccggtg aaatgggcag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagctag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01009575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009575
VERSION     KHUT01009575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggctcga
       61 tcagtctgct gtgaaagtcg ggggctcaac cctcggatgc cggtggatac tgtcgggcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009576
VERSION     KHUT01009576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           86 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggatagg
       61 taagttggat gtgaaagccc cgggctcaac ctgggaatgg cattcaaaac tgcctatcta
      121 gagtgcagta gaggagagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actctctgga ctgacactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009577
VERSION     KHUT01009577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctctc
       61 taagtctggt gtgaaagcct ggggcttaac cccagaagtg cactggatac tggagagcta
      121 gagggcgaga gaggataatg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatctgt ggcgaaggcg attatctggc tcgcatctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009578
VERSION     KHUT01009578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           65 c          104 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 cgcgtccggg gtgaaagccc cccgctcaac gggggaacgg cctcggagac agtcggcctc
      121 gaggggggta ggggcaagcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gcttgctgga ccttcactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009579
VERSION     KHUT01009579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggcgatt
       61 caagtcgggg gtgaaatcct gaggctcaac ctcagaactg ccttcgatac tgcattgctg
      121 gagtatggaa gaggtaggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcctactggt ccatcactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009580
VERSION     KHUT01009580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 gacgtaggag gcaagcgttg ttcggaatta ctgggcgtaa agcgtccgta ggcggcgttg
       61 taagtggata gttaaatccc ggggctcaac cccgtgggtg ctacccagac tgcagcgctg
      121 gaggtcggca gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgtaagcg atcacctggg ctagacctga cgctgaggga cgaaggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009581
VERSION     KHUT01009581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           83 g           50 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcgtaa agcgtgtgta ggcggtttaa
       61 caagtcagga gtgaaatccc agggctcaac cctggaattg ctcttgaaac tgttaagcta
      121 gagtcccgga gaggttagca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgcaagcg gctaactgga cgggcactga cgctgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009582
VERSION     KHUT01009582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgggg
       61 taagtcgggt gtgaaatctc ggggctcaac cccgaaactg cattcgatac tgccttgctc
      121 gaggactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaaggccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009583
VERSION     KHUT01009583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtaaat
       61 taagttagag gtgaaagtcc tgggcttaac ctggggattg cctataatac tgatttgcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga ctcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009584
VERSION     KHUT01009584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           93 g           44 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtccggg gtgaaatcct ccggcttaac cggagaactg ccttggatac tggtggtctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctccactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009585
VERSION     KHUT01009585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta gtcggtattg
       61 agagtcattg gtgaaatccc cgggcttaac ccgggaactg ccagtgagac ctcagtacta
      121 gagtgtgata ggggatagtg gaatgccaag tgtagcggtg aaatgcgtag agatttggcg
      181 gaacaccggt ggcgaaggcg gctatctgga tcacaactga cgatcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009586
VERSION     KHUT01009586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctggg
       61 tcagtctgat gtgaaatctc cgagcttaac tcggaaactg cattggatac tgctcggctt
      121 gagggttgga gggaggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccttgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009587
VERSION     KHUT01009587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          107 g           39 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggcgggaagg
       61 caagtctgct gtgaaagcgc ctggcttaac cgggcgaggt cggcggaaac tgcttacctg
      121 gagggcggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccgcacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009588
VERSION     KHUT01009588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           89 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttacg
       61 taagtcagat gtgaaatccc cgggctcaac ctgggaatgg catttgagac tgcgtggcta
      121 gagtgaatca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg aagtcactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009589
VERSION     KHUT01009589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           99 g           43 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagttcggt gtgaaagccc tcggcttacc tgaggaacgg cgccggaaac tggcagacta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009590
VERSION     KHUT01009590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggcggctttt
       61 caagtcaggt gtgaaagccc acggcttaac cgtggaagtg catttgaaac tgtaaagctt
      121 gagtatggga gaggatggta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gccatctggc ctaatactga cgctgagatg cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01009591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009591
VERSION     KHUT01009591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tgcattgctt
      121 gagtaccaga gagggtagcg gaattccaga agtagaggtg aaattcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctacctgga tggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009592
VERSION     KHUT01009592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           80 g           57 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ctgggcgtaa agggtgtgta ggcgactttt
       61 caagtctgtt gttaaatctc ccagcctaac tgggaaaatg caacggaaac tgattggtta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atatcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgctgataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009593
VERSION     KHUT01009593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctttg
       61 caagtctgac gtgaaatccc tcagctcaac tgaggaagtg catcggaaac tgcaaggctt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009594
VERSION     KHUT01009594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgtctcg
       61 aaagtcgggt gtgaaagccc cgggctcaac ctgggaaggg cattcgaaac ttcgagactc
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009595
VERSION     KHUT01009595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccag
       61 caagtcgggg gtgaaatcgt tcagcccaac tggacaaggg cttccgatac tgttggtctc
      121 gaggggggta ggggtcgggg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg cccgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009596
VERSION     KHUT01009596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           84 g           55 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggtgtaa agggtgcgta ggcggcgata
       61 ttagtctttt gtgaaagccc cgagctcaac ttgggaaacg cagaagaaac ggtattgcta
      121 gagagtgtga gaggtttgca gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaataccgaa ggcgaaggca gcaaactggc acatttctga cgctcaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01009597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009597
VERSION     KHUT01009597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           99 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgccta ggcggttttt
       61 taagtccgct gtgaaagacc gaggctcaac ctcgggatgt cagcggagac tggaagactg
      121 gagtgtcgga gaggagtgcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca gcactctggc cgaagactga cgctgaagcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009598
VERSION     KHUT01009598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggctccg
       61 catgtcggat gtgaaatccc atggatcatc tatggaacgg cattcgaaac tgcggggcta
      121 gagtgcggga gaggaaggtg gaattctcgg tgtagcggtg aaatgcgttg atatcgagag
      181 gaacgccggt ggtgaagacg accttctgga acgcaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009599             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009599
VERSION     KHUT01009599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           46 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tacgtcgact gtgaaaaccc acggctcaac cgtgtggtct gcagccgata cgggcaggct
      121 agagttcggt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgaaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009600
VERSION     KHUT01009600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           85 g           48 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtcatg
       61 aaagtcgggt gtgaaatacc ggagcctaac tccggaacgg cattcgaaac tccatggcta
      121 gagatctgga gaggtaactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaagcg agttactgga cagaatctga cgctgcagca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009601
VERSION     KHUT01009601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggcggcc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tgggtcgctt
      121 gagtcatcca ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaaaaca ggttgctggg gatgttctga cactgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009602
VERSION     KHUT01009602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           87 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcatgta ggcggcatag
       61 taagttcgct gtgaaagccc ttggctcaac caaggaagtg cagcgaaaac tgctaagctc
      121 gagttctgaa gagggtcgcg gaatggccag tgtagaggtg aaattcgtag atattggcta
      181 gaacaccagt ggcgaaggcg gcgacctggg aagatactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009603
VERSION     KHUT01009603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtcttt
       61 taagtctgct gtgaaatccc cgggcttaac ctgggaagtg cggtggatac tggaagacta
      121 gagtgctata ggggtatgcg gaattgccgg tgtagcggtg aaatgcgtag atatcggcag
      181 gaacaccaga ggcgaaggcg gcatactgga gagcaactga cactaaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009604
VERSION     KHUT01009604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggctgtg
       61 caagtcggat gtgaaagccc caggctcaac ctgggaagtg cattcgatac tgcgcagctg
      121 gagtcccgga gaggatggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009605
VERSION     KHUT01009605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           88 g           48 t
ORIGIN      
        1 cacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggttcgta ggcggccacg
       61 caagtggggt gtgaaatccc tcggctcaac cgaggaattg cgctccaaac tgcgtggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009606
VERSION     KHUT01009606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtttgg
       61 taagtgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgcgcaaac tgctgaactt
      121 gagcatacta gaggcaggtt gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg gtatagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009607
VERSION     KHUT01009607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcga
       61 taagtctact gtgaaagtca agggctcaac ccttgaaagc cggtggatac tgtcagacta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009608
VERSION     KHUT01009608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcaata
       61 caagtcgcat gtgaaatacc agggctcaac cctggggggc catgcgaaac tgtatagctt
      121 gagtgcagta gaggtaagcg gaattcctgg tgtagcggta aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcttactggg ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009609             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009609
VERSION     KHUT01009609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           91 g           54 t
ORIGIN      
        1 tacgaaggtt gctagcgttg ttcggaatca ctgggcttaa agcgcatgta ggcggctcat
       61 caagtgcctt gtgaaatccc acggctcaac cgtggaattg cttggtatac tgttcgagct
      121 tgaggcaggt aggggcacgt ggaactcttg gtggagcggt ggaatgcgta gatatcaaga
      181 ggaacgccgg tggcgaaagc ggcgtgctgg gcctgtcctg acgctgagat gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009610             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009610
VERSION     KHUT01009610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 gaagttgggt gtgaaagccc tcagctcaac tgaggaattg cgcccaaaac tgccgcactt
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctagg
      181 gaacacctgt agcgaaagcg gctcactggg tctcttctga cgctgagtgc acgaaagcta
      241 ggggagcgaa gagg
//
LOCUS       KHUT01009611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009611
VERSION     KHUT01009611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c           94 g           52 t
ORIGIN      
        1 gacggaggcc ccgagcgttg ttcggaatta ttgggcttaa agcgcgtgta ggcgggccgg
       61 taagtgcttt gtgaaatccc cgggctcaac ccgggaattg cttggcatac tgtcggtctt
      121 gaggcaggta tgggtcactg gaaccttagg tgtagcggtg aaatgcgtag atatctaagg
      181 gaacgccggt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01009612             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009612
VERSION     KHUT01009612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatta ctgggcataa agcgtgcgta ggcggcggcg
       61 taagtgtagg gtgaaatccc acggcccacc cgtgtgaaca gccccgcata ctgcgttgct
      121 tgagtgtggt aggggagagc ggaactcctg gtggagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgcaggc ggctctctgg tccacaactg acgctgaagt acgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01009613             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009613
VERSION     KHUT01009613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c           93 g           51 t
ORIGIN      
        1 taccggcggc tcgagtggtg gccgatatta ttgagtctaa agggtccgta gccggctttg
       61 caagtctctc gggaaatcca gcggcttaac cgttggtcgt ccggggggta ctacattgct
      121 tgggactggg agaggtagga ggtactcagg gggtaggagt gaaatcctgt aatcctttgg
      181 ggaccaccgg tggcgaaggc gtcctaccag aacaggtccg acggtgaggg acgaaagcta
      241 ggggcacgaa ccgg
//
LOCUS       KHUT01009614             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009614
VERSION     KHUT01009614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcggtacgg
       61 caagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaaaac tgccgaacta
      121 gagtgtggga gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctggc ccatttactg acgctcagac acgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01009615             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009615
VERSION     KHUT01009615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           83 g           54 t
ORIGIN      
        1 tacgagtgct tcgagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggtagtg
       61 ttagtcttgt gttaaatctc ttggctcaac cgagaggctg cataagaaac ggcacaacta
      121 agaggacgga agaggtctct ggaactcatg gtgtagcggt gaaatgcgtt gatatcatgg
      181 ggaacaccaa aagcgaaggc aagagactgg tccgctcctg acgctgaaac acgaaagcgt
      241 gggtcgcgaa tggg
//
LOCUS       KHUT01009616             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009616
VERSION     KHUT01009616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           84 g           50 t
ORIGIN      
        1 tacaggtggt acgagtagta tccacgaata ttgagtctaa agcgctcgta gcaggcttgg
       61 acagttctct gtgaaatctt caggctcaac ctgaaggcgc gcagggaata ctaccaggct
      121 tgggagtggg ggaggccaag ggtacttgag ggggaggagt aaaatcctgt aatccttcaa
      181 ggaccttcag tagcgaaggc gcttggccaa aacacgtccg actgtgagga gcgaaggcta
      241 ggggagcaaa tcgg
//
LOCUS       KHUT01009617             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009617
VERSION     KHUT01009617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gctttgcata ctggcagact
      121 ggagggacgc agaggtcaac agaattcccg gtgtaccggt gaaatgggta gatatcggga
      181 ggaataccga tggcgaaggc agttgactgg gcgtttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01009618             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009618
VERSION     KHUT01009618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           91 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcctga
       61 caagtcagag gtgaaagccc ggagcttaac tccggaattg cccctgagac tgccaggctt
      121 cgagtacggg agaggtgagt ggaattccga gtgtagaggt gaaattcgta gatattcgga
      181 agaacaccgg tggcgaaggc ggctcactgg cccgttactg acgctcaggc gcgacagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01009619             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009619
VERSION     KHUT01009619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           54 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggattta ttgggcgtaa agggcgcgta ggcgggcgaa
       61 ttagtccggt gtgaaatctt caccgctcaa cgggaagagg tcactggata ctgttcgtct
      121 tgaggtcgtt agaggataac agaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaataccga ttgcgaaggc ggttatctgg gacgatcctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009620             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009620
VERSION     KHUT01009620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           48 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtggt gtgaaatctc cagggctcaa cccggaaact gcaccgcata ctggcagact
      121 ggagggatgc agaggtgagt ggaattcctg gtgtaccggt gaaatgggta gatatcagga
      181 ggaacacccg tggcgaaggc ggctcactgg gcatctcctg acgctgaggc gcgaaagtgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009621
VERSION     KHUT01009621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggtggcgatt
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaactg cattcgatac tgggttgcta
      121 gagatcggta gagggcagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccgat ggcgaaggca actgcctggg cctgatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009622
VERSION     KHUT01009622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c          102 g           49 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtggc
       61 taagtccgct gtgaaagcct gaggctcaac ctcagagggt cggtggatac tggctgtctt
      121 gaagaggcta ggggaagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacacccgt ggcgaaggcg gtcttctggg gtcttcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009623
VERSION     KHUT01009623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcgt
       61 acagtctagg gtgaaatact ccggcttaac cggagaactg ccttggatac taacgatctc
      121 gagggaggca ggggcagttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aactgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009624
VERSION     KHUT01009624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           88 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttacc
       61 taagtctgat gtgaaagccc agggcttaac cctggaagtg cattggatac tgggtcacta
      121 gagggtaaga gaggaaggcg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gctttctggc ttatttctga cgctcagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009625
VERSION     KHUT01009625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           88 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggctgttgag
       61 taagtcttct gtgaaatcta cgggcttagc ctgtaaagtg cagaggaaac tacttggctt
      121 gagtgtcgga gaggataggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009626
VERSION     KHUT01009626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           90 g           45 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggcatat
       61 caagtctgcg gttaaaggtt ctggctcaac cagagacagg ccgtggaaac tgataagcta
      121 gagtatggta ggggcagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccaaaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01009627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009627
VERSION     KHUT01009627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgcgtgta ggcggtctgt
       61 taagtcgggt gtgaaatctc cgggcccaac ccggaaattg catccgatac tggcagactt
      121 gaggacggta gaggttggtg gaatagccgg tgtaccggtg aaatgggcag atatcggcta
      181 gaacaccagt ggcgaaggcg gccaactggg ccgttcctga cgctgagacg cgaaagccag
      241 gggagcgaac cgg
//
LOCUS       KHUT01009628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009628
VERSION     KHUT01009628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtggattgg
       61 taagtcagtg gtgaaagccc cgagctcaac tcgggaattg ccgttgatac tgctaatctt
      121 gagtacggtt gaagtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgtaggca gctcactaag ccgtaactga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009629
VERSION     KHUT01009629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtcgga
       61 taagtctgat gtgaaatctc agagctcaac tctgaaactg cattggatac tattcggctt
      121 gaggtctgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caggtcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009630
VERSION     KHUT01009630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtacag
       61 taagtctgat gtgaaagccc caggctcaac ctgggaagtg cattggatac tgctgaactt
      121 gaatctggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaagattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009631
VERSION     KHUT01009631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           93 g           50 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggaatca ctgggcgtaa agagctcgta ggcggggtcg
       61 cgcgtcgtgg agtaaatttc tatgctcaac gtagacatct tctgcgatac ggcgactctt
      121 gaggacagga gaggaaagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gctttctggc ctgattctga cgctgaggag cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009632
VERSION     KHUT01009632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           96 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgata
       61 agggtgtgat gtgaaagccc tgggcttaac ccgggaagag catcgcaaac catatgactg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttgatactga cgctgaagcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009633
VERSION     KHUT01009633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           90 g           46 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctcgg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac ttccaagctc
      121 gagtcctgga gaggaaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009634
VERSION     KHUT01009634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgaacgta ggcggctatg
       61 taagtcgggt gtgaaagccc agagctcaac tctggaagtg cattcgaaac tgcatggctt
      121 gagtagcgga gaggatagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgcatactga cgctgaagtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009635
VERSION     KHUT01009635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ttgggcgtaa agcgtgcgca ggcggttgta
       61 taagatagat gtgaaatcct tgggctcaac ctgagaactg catttatgac tgtataacta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009636
VERSION     KHUT01009636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 gacagaggtt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtgaag
       61 tatgtctgac gtgaaatccc tcagcttaac tgaggaagag catcggaaac tgcttcactg
      121 gagtgcgaga gagggtggcg gaattcccag agtagaggtg aaattcgtag atactgggag
      181 gaacacctgt ggcgaaggcg gccacctggc tcgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009637
VERSION     KHUT01009637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtaagt
       61 taagtctcat gtgaaagctc cgagctcagc ttggaaagtg catgggaaac tggcatactt
      121 gagtgtcgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggt cgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009638
VERSION     KHUT01009638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           83 g           56 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggtgtat
       61 taagtctgtt gttaaatctt ctggcctaac cggaaatcag cagtagatac tggtgcacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009639
VERSION     KHUT01009639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           98 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggtatct
       61 cacgtctggg gtgaaagccc agggctcaac cctggaacgg ccttggaaac ggaggtactt
      121 gagcgtcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg ccttgctggc cgattgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009640
VERSION     KHUT01009640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           78 g           57 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggttgcttca
       61 ttagtcttct gtcaaattct tcggctcaac cgggggtccg cagaagaaac ggtgaagcta
      121 gaggatgtga gaggtgaata gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaataccaaa agcgaaggca attcactggc acattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01009641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009641
VERSION     KHUT01009641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           52 c           88 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ttgggcgtaa agggtgtgca ggcggcttac
       61 caagttcgcg gcgacagcca ttggcttaac cgatggagga ctgcgaaaac tggtaaactt
      121 gagagcgaga gagggacatg gaactccggg tggagcagtg aaatgcgtag agatccggaa
      181 gaacaccaac ggcgaaggca atgtcctagc tcgtatctga cgctcaagca cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009642
VERSION     KHUT01009642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           90 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 caagtcggaa gttaaaacag aaggctcaac cttctgccgc tttcgatact gctatggctt
      121 gagtctggta ggggatgacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg gtcatctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009643
VERSION     KHUT01009643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          107 g           36 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcccct
       61 taagcgtgtg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tggggggctg
      121 gagcacgtca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg acgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009644
VERSION     KHUT01009644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgct
       61 caagtcgggg gtgaaatctt tccgcttaac gggaaaagag ctttcgatac tgggcggctg
      121 gagggaggta ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga cctccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009645
VERSION     KHUT01009645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgagta ggcggtttta
       61 caagtcagat gtgaaatgca tgggctcaac ccatggactg catttgaaac tgtgagactt
      121 gagtgcggta gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctggg ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009646
VERSION     KHUT01009646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtttgg
       61 aaagtctgtg gtgaaatctc tcggctcaac cgagaggggt ctgcagatac tcccagactt
      121 gagtgccgta gacgaagatg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gtcttcgagg cggtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009647
VERSION     KHUT01009647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c           92 g           42 t
ORIGIN      
        1 gacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaacgg ctgggcatac tggcggtctt
      121 gagctgccta ggggcaactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaac ggtgaaaaca ggttgctggg ggcatgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009648
VERSION     KHUT01009648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgccctg
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cacccgatac tgcagggcta
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009649
VERSION     KHUT01009649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           84 g           53 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtttgg
       61 aaagttggat gtgaaatcct gcagcttaac tgcagaaccg cattcaaaac ttccgaactc
      121 gagtttgaga ggggtaaagg gaattcccgg tgtaagggtg aaatctgcag atatcgggaa
      181 gaacaccagt cgcgaaggcg ctttactggc tcacaactga cgctgaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009650
VERSION     KHUT01009650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           96 g           47 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttgta
       61 caagttggtt gtgaaagcgc ccggctaaac tgggcgaggc cgatcaagac tgtataactt
      121 gagagtgtta gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggggaagccg gcgccctggg acatatctga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009651
VERSION     KHUT01009651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           83 g           50 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggcctga
       61 taagtctgtt gtgaaagctc ttggcttaac tgagagaggt caacagaaac tattaggctt
      121 gagtatagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaac ggcgaaagca ggtacctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009652
VERSION     KHUT01009652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           86 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttatg
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaattg catttgagac tgcatagcta
      121 ggatgcggaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctggt ccagcatcga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009653
VERSION     KHUT01009653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           95 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtttgg
       61 taagatgggt gtgaaagctc ctggctcaac tgggagaggt cgctcatgac tgccagactg
      121 gagagtggta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ccatttctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009654
VERSION     KHUT01009654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           99 g           40 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattaa ctgggcgtaa agcgcacgca ggtggtcgtg
       61 caagtgtcgt gtgaaagcgg cccgcttaac gggtcgaggc catggcagac tgcgcgactg
      121 gagccgtgta gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca acggactggg cacgtgctga cactgaggtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01009655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009655
VERSION     KHUT01009655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c           99 g           38 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcccgg
       61 aaagtcggat gtgaaaaccc ggggctcaac cccgggcctg catccgaaac ttccgggcta
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009656
VERSION     KHUT01009656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          102 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 tgcgtcgggt gtgaaagccc cgggctcaac ctgggaggtg cactcgatac gggcgggctg
      121 gaatccggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggc ccggaattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009657
VERSION     KHUT01009657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           84 g           50 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgtt gtgtaagctc ccggcttaac cgggagaggt caacggaaac tatcagactt
      121 gagtatagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009658
VERSION     KHUT01009658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgtgca ggcggtctat
       61 caagtccgat gtgaaagccc cgggcctacc ccgggaagtg cattggaaac tggtagactt
      121 gaatacggca gggggcagtg gaattcctgg tgtagaggtg aaattcgcag atatcaggaa
      181 gaacaccagt ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009659
VERSION     KHUT01009659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          102 g           38 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggcgcgc
       61 caagcacgat gtgaaagctc ctggctcaac tgggagagtg cgttgtgaac tggcgggcta
      121 gagggcaggc gacggcggtg gaattcccgg tggagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccgccgagc ctgtatctga cgctgaagcg cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009660
VERSION     KHUT01009660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctggg
       61 taagttcggt gtgaaagctc ggagctcaac tccgaaatgg catcgaatac tatttggcta
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009661
VERSION     KHUT01009661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctag
       61 caagtcggat gtgaaagccc ttggctcaac tgaggaagtg cattcgaaac tgcgagactt
      121 gagtcccgga gaggatggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009662
VERSION     KHUT01009662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           96 g           37 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggaggtg
       61 caagtcactt gtgaaagcgc ccggctcaac cgggcgagga cgagtgaaac tgcacgacta
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctgga ccgaccctga cgctcagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009663
VERSION     KHUT01009663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           86 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatca ctgggcgtaa agggtgcgta ggtggtcaaa
       61 taagtcagat gtgaaatccc tgggctcaac ctaggcattg catttggtac tgtttgacta
      121 gagtgcagta gaggaatgtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acattctgga ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009664
VERSION     KHUT01009664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgtgtgca ggcggacctg
       61 taagtttgat gtgaaatctc ctggctcaac tgggagaggc cgttgaatac tgtaggtcta
      121 gagggcaaca gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctggg ttgcatctga cgctcagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009665
VERSION     KHUT01009665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           92 g           40 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaacgg ctgggcagac tggcggtctt
      121 gagctgccta ggggcgactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggtgaaaaca ggtcgctggg ggcaagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009666
VERSION     KHUT01009666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           84 g           53 t
ORIGIN      
        1 tacgtaggac gcaaacgttg tccggattta ctgggtgtaa agggtgctca ggcggtcttg
       61 caagtcagtg gtgaaatccc ggagctcaac tccggaactg cctttgatac tgtgaggctt
      121 gagtgcagaa gaggcggatg gaatttctgg tgtagcagtg aaatgcgtag atatcagaaa
      181 gaacaccagt ggcgaaggcg gtccgctggt ctgtcactga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009667
VERSION     KHUT01009667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtttgc
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tggcgaactt
      121 gagtatcgga gaggttggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaatacctgc ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009668
VERSION     KHUT01009668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           96 g           45 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggttgct
       61 ccagtccgct gtgaaatctc ctggctcaac caggataggt cggtggaaac taggcaactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009669
VERSION     KHUT01009669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggctaga
       61 taggtccgtt gtgaaaactc gaggctcaac ctcgagacgt cgatggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009670
VERSION     KHUT01009670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c          104 g           33 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggttact
       61 gacgtgccgt gtgacaaacg ggggctcaac ccccgagggc cacggtaaac ggggcgactg
      121 gaggtaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctatcctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01009671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009671
VERSION     KHUT01009671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           94 g           53 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ttgggcgtaa agcgcacgca agcggttttg
       61 caagtctgac gtgaaatctc ctggcttaac tgggagcggt cgttggaaac tgcagagctt
      121 gaggcgatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag agatcggggg
      181 gaatacctgt ggcgaaagcg gcaccctggc gtcgttctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009672
VERSION     KHUT01009672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           92 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agagcgcgta ggtggtttgc
       61 taagtctggt gtgaaagccc tcggcttaac cgaggaagtg cattggatac tggcaagctt
      121 gagtgtgaga gaggattgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcaatctgga tcataactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009673
VERSION     KHUT01009673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          100 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcgcgt
       61 taagtctgac gtgaaagaac ctggcttaac tgggggaggt cgttggagac tggcgtgctt
      121 gaggacatga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaagcg gcaccctggc atggacctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009674
VERSION     KHUT01009674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctat
       61 taagttgggt gtgaaatctt cgggctcaac ccggaaactg cactcaaaac tggtggcctt
      121 gaggtatgga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catatcctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009675
VERSION     KHUT01009675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 tacgaaggtg gctagcgtta ctcggaatca ctgggcataa agggcgtgta ggcgggtgcg
       61 taagtccggt gtaaaatccc ctggctcaac cagggaagtg cattggatac tgcgtgcctt
      121 gagtgcggga ggggagagtg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg gctctctggc ccgtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009676
VERSION     KHUT01009676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           97 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctgtt
       61 taagtccgat gtgaaagccc tgggctcaac ccgggaagtg cattggaaac tggacagctt
      121 gagtacggga gagggaggtg gaattccgag tgtaggggtg aaatccgtag atattcggag
      181 gaacaccggt ggcgaaggcg gcctcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009677
VERSION     KHUT01009677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           41 c           97 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatga ctgggcgtaa agggcgcgta gacggctatt
       61 taagtctgga gtgaaagtcc tgctttcaag gtgggaattg ctttggagac tggatggctt
      121 gagtgcggaa gaggtaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccagt ggcgaaggcg acttactggg ccgtaactga cgttgatgcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009678
VERSION     KHUT01009678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           90 g           43 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggacggc
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaaagg ctgggcatac tggtcgtctt
      121 gagccatcca ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggcgaaagca ggttgctgga gatgcgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009679
VERSION     KHUT01009679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           86 g           50 t
ORIGIN      
        1 tacccgcgcc ccaagtggtg gtcgatatta ttgagcctaa aacgttcgta gccggtcttc
       61 taaatccttg ggtaaatcgg gcagcttaac tgtccgaatt ccggggagac tgggagactt
      121 gggaccggga gaggtcagag gtacttctgg ggtaggggta aaatcctgta atcctggaag
      181 gaccaccggt ggcgaaggcg tctgactaga acggatccga cggtgaggaa cgaagccctg
      241 gggcgcaaac ggg
//
LOCUS       KHUT01009680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009680
VERSION     KHUT01009680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctctgatac tggcaggctc
      121 gagcgaggta ggggcatgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009681
VERSION     KHUT01009681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          100 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgca ggcggtgact
       61 taagtctgct gtgaaagctc ctggctcaac tgggagaggt cggtggaaac tgggttacta
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009682
VERSION     KHUT01009682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           98 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 tgcgtcgacg gtgaaaaccc ccagctcaac tgggggcctg ccgtcgatac gggcagactt
      121 gagtgcggta ggggagaccg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgtcactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009683
VERSION     KHUT01009683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           90 g           43 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatgct
       61 taagtgcctt gtgaaatccc acggcttaac cgtggaactg ctgggcatac tgggcgtctt
      121 gaggtgccta ggggtcgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggcgactgga ggcaaactga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009684
VERSION     KHUT01009684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtcgga
       61 caagtcactt gtgaaatctc cgggcttaac ccggagcggc caagtgatac tgtctaactt
      121 gagtgcggaa ggggctactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggtagctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009685
VERSION     KHUT01009685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcgcgta ggcggctatg
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgctcgatac tgcatggctt
      121 gaggtctgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cagaccctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009686
VERSION     KHUT01009686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcagaa gtgaaagccc cgggctcaac ccgggaatag cttttgatac tgttgagctc
      121 gagtccggga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009687
VERSION     KHUT01009687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           39 c           69 g           62 t
ORIGIN      
        1 tacatagggt gcaagcgttg ctcggaatta ctgggcgtaa agaacatgta ggaggatttt
       61 taagtcgtta gtgcaatccc aaggctcaac cttggaactg ctgacgatac taaaaatcta
      121 gagtaagata gagggtgata gaattcttag tgtaggggtt aaatccatag atattaagag
      181 gaataccgaa agcgaaagca gtcacctggg tctttactga ctctaaagtg tgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009688
VERSION     KHUT01009688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaactgc ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcatgt
       61 caagtcaggg gtgaaatccg gcagctcaac tgtcgaagtg cctttgatac tgacgtgctc
      121 gagggaggta ggggtgtgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009689
VERSION     KHUT01009689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           93 g           46 t
ORIGIN      
        1 tacaggggtc tcgagcgtta atcggaatca ctgggcttaa agggagcgta ggctgaccag
       61 caagtgcttt gtgaaagccc ccggctcaac cggggaactg cggggcatac tgctggtctt
      121 gaggcatgta ggggcgggca gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggtgaagacg gcccgctggg catgtcctga cgctcaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009690
VERSION     KHUT01009690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggccgactgt
       61 taagtcgggg gtgaaagcct ggagctcaac tccagaactg cctccgatac tggtagtctt
      121 gagtccgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009691
VERSION     KHUT01009691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           77 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggcttt
       61 taagtcagtg gtgaaaactt cgagcttaac tcgaaaactg ccattgatac tattagtctt
      121 gaatttagtt gaggttggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctggctaaa ctaaaattga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009692
VERSION     KHUT01009692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           72 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttag
       61 taagtcagtg gtgaaatccc atcgctcaac ggtggaatgg ccattgatac tgctgaactt
      121 gaataattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactact aattcattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009693
VERSION     KHUT01009693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtcagc
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tagctgactt
      121 gaatactgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009694
VERSION     KHUT01009694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtctgat gtgaaatctc cgggctcaac tcggaaatgg cattggaaac taccttgcta
      121 gaggattgga ggggggactg gaatgctcgg tgtagcagtg aaatgcgtag atatcgagcg
      181 gaacaccagt ggcgaaggcg agtccctgga caattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009695
VERSION     KHUT01009695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           87 g           46 t
ORIGIN      
        1 tacagagact gcaagcgtta ctcggattca ctgggcgtaa agggtgcgca ggcggctaat
       61 tgtgtcaggt gtgaaatccc ggggcttaac tccggaactg cgcctgaaac tggttagcta
      121 gaggctcgga gaggtaagcg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccaaa ggcgaaggca gcttactgga agagatctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01009696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009696
VERSION     KHUT01009696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ttgggcgtaa agcgcacgca ggcggtcaga
       61 tcagtctgaa gtgacagtcg tcggcttaac cgacgaagtg tttcggatac tgtctgactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccgat ggcgaaggca gtgtcctggg aagcatctga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009697
VERSION     KHUT01009697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           86 g           58 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggtgcgta ggtggtttga
       61 taagttaggt gtgaaagtcc tgggctcaac ctggggattg cgcttaatac tgtcaagcta
      121 gagtattgta gaggaaagtg gaatttccgg tgtagcggta aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctttctgga caaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009698
VERSION     KHUT01009698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacagggggc tcgagcgtta atcggaatca ttgggcttaa agggtgcgta ggcgggtcgc
       61 taagtgtcct gtgaaatccc ttggctcaac caaggaatcg caaggcatac tggcggcctt
      121 gaggcatgta ggggcggacg gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg gtccgctggg catgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009699
VERSION     KHUT01009699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcagat
       61 caagtcagct gtgaaatctc cgagcttaac tcggaacggt cagctgatac tgttctgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009700
VERSION     KHUT01009700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcgggctgg
       61 caagtcaggg gtgaaatctc acggctcaac cgtgaaactg cctttgatac tgccagtctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc ccggaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009701
VERSION     KHUT01009701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           92 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggtgggttat
       61 taagtcttgt gtctaagtgc ggagctcaac tccgtatagg cgcaggaaac tgatgatctt
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtgtctga cactgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009702
VERSION     KHUT01009702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtgagt
       61 taagtctgct gtgaaatcta cgggcttagc ctgtaacgtg cagcggaaac tggctgactt
      121 gagtatcgga gagggcaggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgcctggc tgattactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009703
VERSION     KHUT01009703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggttggta ggtggtctcg
       61 tttgtcaggg gtgaaagcct tgggcttaac ccaagaagtg cctctgaaac ggcgagactg
      121 gagtactgga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gctcactgga cagttactga cactgaacaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009704
VERSION     KHUT01009704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcga
       61 tcagtccgct gtgaaagtcc agggctcaac cctggaatgc cggtggatac tgtcgggcta
      121 gaatccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009705
VERSION     KHUT01009705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           99 g           46 t
ORIGIN      
        1 gacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcgact gtgaaatccc gcagctcaac tgcgggcttg cagccgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009706
VERSION     KHUT01009706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c          100 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggagtgc
       61 tttgtcgggc gtgaaattcc agggctcaac cttggggctg cgttcgagac gggtgctctt
      121 gagtttggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccaagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009707
VERSION     KHUT01009707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           94 g           50 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ctgggcgtaa agagctcgta ggcggggacg
       61 cgcgtctcgg agtaaatttc agggctcaac cctgaagctt tccgggatac ggcgtctctt
      121 gagggcagaa gaggagaacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttctctggt ctgtctctga cgctgaggag cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009708
VERSION     KHUT01009708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           83 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgca ggcgggatag
       61 aaagtcaagt gtgaaatccc ggagcttaac tccggaacgg cacttgaaac tcctattcta
      121 gagggtaggc ggagaaaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaagcg attttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009709
VERSION     KHUT01009709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           38 c           85 g           58 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtttgta ggcggttgaa
       61 caagtcagat gtgaaagccc ggggcttaac cccggaattg catttgaaac tgttcagcta
      121 gagagtgtta gaggaaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gctttctggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009710
VERSION     KHUT01009710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttcat
       61 taagttaggt gtgaaagccc tgggcttaac ctgggaatgg catttaagac tggtgaacta
      121 gagtagagta gaggggagca gaatttccgg agtagcggtg aaatgcgtag atatcggaaa
      181 gaataccgga ggcgaaggcg gctccctggg ctcatactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009711
VERSION     KHUT01009711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatcag
       61 tcagttagag gtgaaagccc agggctcaac cctggaactg cctttaatac tgctggtctg
      121 gaggtcgaga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcgctggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009712
VERSION     KHUT01009712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacccg
       61 caagtccggg gtgaaatcgt tcggctcaac cggacaattg cctcggatac tgcaggtctg
      121 gagggaggca ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009713
VERSION     KHUT01009713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           91 g           42 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgcgca ggtggtccga
       61 taagtcgggt gtgaaatccc ggggctcaac tccggacgtg cacacgatac tgtcggactg
      121 gagtactgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagctactga cactgaggcg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01009714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009714
VERSION     KHUT01009714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgccg
       61 ttagtcaggt gtgaaatctc ggggctcaac cccgaaactg cacttgatac tgcggtgctc
      121 gaggactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaaggccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009715
VERSION     KHUT01009715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttagt
       61 taagccagct gtgaaatccc cgggctcaac ctgggcactg cagttggaac tggctagcta
      121 gagtatggta gaggggtgtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg gcaccctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009716
VERSION     KHUT01009716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           61 c           80 g           41 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agagcgaaaa gccggtcgca
       61 ttagtctgat gtcaaaccat ggggcccaac cccatgcctg catcggaaac ggtgcgactc
      121 gagcgggtga gaggtaggcg gaacgtacgg tgtaggggtg aaatccactg atatcgtacg
      181 gaacaccaaa agcgaaggca gcctactagc gctctgctga cggtgcatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01009717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009717
VERSION     KHUT01009717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtttgt
       61 taagtcttct gtgaaatcta cgggcttagc ctgtaaagtg cagaggaaac tagcaggctt
      121 gagtgtcaga gaggataggg gaatacccgg tgtagaggtg aaattcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg cctatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009718
VERSION     KHUT01009718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           38 c           78 g           57 t
ORIGIN      
        1 gacgtaagtg gcaagcgtta tccggaatta ttgggcgtaa agggcgcgta ggcggttaaa
       61 taagtcagag gtgaaatcac caagcttaac ttggatgaag cctttgaaac tgtttaacta
      121 gagtatagga taggaagatg gaattatcac tgtagcggtg gaatgcgtag atagtgatag
      181 gaacaccaat gacgtaggta gtcttctgga ctaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009719
VERSION     KHUT01009719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           52 c          106 g           44 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggcgcgca ggtggtgcgg
       61 taagtctgcc gtgaaatctc ccggctcaac tgggagggtg cggtggagac tgccggacta
      121 gaggcactta gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ggtgacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009720
VERSION     KHUT01009720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           94 g           47 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcag
       61 caagttgctc gtgaaatctc tgggctcaac ccagagtctg cgggcaaaac tgctgtgctt
      121 gagtgtggaa gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009721
VERSION     KHUT01009721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggcctga
       61 taggtcggat gtgaaatccc tcggctcaac caaggaagtg cattcgaaac catcaggctg
      121 gagtcccgga gaggaaggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaagcg gccttctgga cgaggactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009722
VERSION     KHUT01009722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           83 g           56 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccggagat
       61 taagcgtgtt gtgaaatgta gacgctcaac gtctgcactg cagcgcgaac tggtttcctt
      121 gagtacgcac aaagtgggcg gaattcgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gctcactgga gcgcaactga cgctgaagct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01009723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009723
VERSION     KHUT01009723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           89 g           44 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaagag
       61 taagtacctt gtgaaatccc acggctcacc cgtggaaccg ctgggtatac tgctcttctt
      121 gggccattca ggggcgactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggtgaaaaca ggtcgctggg aatgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009724
VERSION     KHUT01009724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           90 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtgggaagt
       61 caagtctggt ttgaaagctg gcggctcaac cgtccgatgt ggctggaaac tgactttctt
      121 gaatggagta gcggctgccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggcagctggg ctctgattga cactgaggca cgagagcatg
      241 ggtagccaac agg
//
LOCUS       KHUT01009725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009725
VERSION     KHUT01009725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           62 c          116 g           33 t
ORIGIN      
        1 cacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcccag
       61 tgggcctggc gtgaaagggt gccgctcaac ggtgccaggg cgtcggggac cgctgggctt
      121 gagggcggga ggggccggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccggctggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009726
VERSION     KHUT01009726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           88 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcgcgta ggtggctgtt
       61 taagttatgt gtgaaatccc tgggcttaac ctaggaattg catataagac tggatagcta
      121 gagttcgaga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc tcgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009727
VERSION     KHUT01009727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggattgg
       61 taagtcaggg gtgaaatgcc gaggctcaac ctcggaactg cctttgatac tgctgatctg
      121 gagtacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009728
VERSION     KHUT01009728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           88 g           54 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggatcta
       61 taagtcagga gtgaaagccc cgagcttaac ttgggaagtg cttttgatac tgtagatctt
      121 gaatgtggga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ccaacattga cactgagacc cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01009729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009729
VERSION     KHUT01009729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           84 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagtcgagt gtgaaatccc caggcttaac ctgggaactg ctctcgagac tgcatagcta
      121 gagtatggga gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009730
VERSION     KHUT01009730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggatctt
       61 taagtcagag gtgaaagccc aaggctcaac cttggaattg cctttgatac tgaagatctt
      121 gagaccggaa gaggttagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctaactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009731
VERSION     KHUT01009731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggttcag
       61 taagtccgct gtgaaagtcc agggctcaac cctggaatgc cggtggatac tgctgaacta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009732
VERSION     KHUT01009732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggcggatttt
       61 taagtcaggt gtgaaatctc ggggcttaac ctcgaacgtg cgcctgaaac tgataatcta
      121 gaataccgga gagggaaggg gaatttctca tgtaggggta aaatccgtag atatgagaag
      181 gaacaccaga ggcgaaggcg ccttcctaga cggttattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009733
VERSION     KHUT01009733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcccgg
       61 tgtgtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgccgggctt
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggaactga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009734
VERSION     KHUT01009734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcggag
       61 taagtcggat gtgaaatttg gaggctcaac ctccaacttg catctgatac tgcttcgcta
      121 gagacctgga gaggagtttg gaattctcgg tgtagcagtg aaatgcgtag atttcgagaa
      181 gaacgctcgc agcgaaagcg aaactctgga cagcgtctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009735
VERSION     KHUT01009735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           99 g           41 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcggtcgtg
       61 tacggcggtg gtgaaagctc ccggcttaac tgggagaggc ccaccgcaac ggcatgactc
      121 gaggatggta gaggcagatg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gtctgctggg ccatatctga cgctgaggag cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009736
VERSION     KHUT01009736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcgggcgtg
       61 taagtcattt gtgaaatccc tgggcttaac ctggggtctg caactgatac tgcacgtctt
      121 gaatgcctga gagggtggta gaattccagg agtagaagtg aaattcgtag atatctggag
      181 gaatacctga ggcgaaggcg gccacctggc aggacattga cgctgaggcg cgaaagcacg
      241 gggagcaaac agg
//
LOCUS       KHUT01009737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009737
VERSION     KHUT01009737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcccgca ggcggtccaa
       61 taagttcttg gtgaaatctc ctggctcaac tgggagggtg cctggaaaac tattggactt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01009738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009738
VERSION     KHUT01009738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggactcg
       61 caagtgcttt gtgaaatccc acggctcaac cgtggaacgg cttggcagac tgcgggtctc
      121 gaggttggta ggggccgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcggctggg ccaattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009739             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009739
VERSION     KHUT01009739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           48 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtcta ggtggcctgg
       61 caagtcagat gtgaaatccc tcggctcaac cgaggacgtg catttgaaac tgctatggct
      121 tgagtgtcgg agggggtagc ggaattcccg gtgtagaggt gaaattcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggctacctgg ccgaacactg acactgaagc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009740
VERSION     KHUT01009740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccaag
       61 cgggtcggat gtgaaagccc tcggcttaac cgaggaagtg cttccgaaac tatttggctt
      121 gagtacggga gaggagggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctctctgga ccgtaactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009741
VERSION     KHUT01009741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          101 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggtcctt
       61 taagtgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcaaac tgggggactt
      121 gagggcgtga gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggc acgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009742
VERSION     KHUT01009742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtctcg
       61 aaagtcggac gtgaaagccc cgggctcaac ccgggaagtg cgctcgatac tgcgagactc
      121 gagtgtcgga gaggttggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaataccggc ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009743
VERSION     KHUT01009743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          108 g           36 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 taaggtcgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtggcaac tgcgaggctt
      121 gagcacagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009744
VERSION     KHUT01009744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           82 g           57 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggttgtcttg
       61 ttagtcttct gtgaaattct tcggctcaac cgggggtccg caggggaaac ggcaagactt
      121 gaggatgtga gaggtgaatg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcattcctga cactcaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01009745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009745
VERSION     KHUT01009745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           93 g           55 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttcat
       61 taagtttggt gttaaagatc ggggctcaac cccgggagtg cactgaatac tggtggactg
      121 gagtgtggta gaggctagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaggcg actagctggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01009746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009746
VERSION     KHUT01009746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           83 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcaa
       61 aaagtcagat gtgaaatccg gaggctcaac ctccgaattg catccgaaac tcttgtgctt
      121 gaggattgta gaggagcttg gaactctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgc agcgaaagcg aggctctggg caatttctga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009747
VERSION     KHUT01009747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           85 g           55 t
ORIGIN      
        1 tacggagggt gcaaacgtta tccggaatca ctgggcgtaa agagcatgta ggtggttgtg
       61 taagtgggcg attaaagacc gatgcctaac atcgggcctg ttgcctatac tgcacgactt
      121 gaggatggca ggggtaagcg gaactttagg tgtaggagtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttactggg ccatttctga cactgagatg cgaaagcgtg
      241 gggagcgatt ggg
//
LOCUS       KHUT01009748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009748
VERSION     KHUT01009748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcgggagag
       61 taagtcaaat gtgaaagccc cgggcttaac ctgggaatgg catatgaaac tgtttttcta
      121 gagtgaagta gagggtgacg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gtcacctgga cttttactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009749
VERSION     KHUT01009749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcggcg
       61 ttagtcgggt gtgaaatccc tcggctcaac cgaggaagtg cactcgatac tgcgttgctt
      121 gagtactgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cagtcactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009750
VERSION     KHUT01009750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcaggg gtgaaatccc agagcttaac tctggaattg cctttgaaac tgtaaagcta
      121 gagtactgga ggggttggcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gccaactgga cagtaactga cgctctggcg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01009751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009751
VERSION     KHUT01009751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ctcggtatca ctgggcttaa agggcgcgta ggcggtgcgg
       61 tacggtttcg gtgaaatccc tcggcccaac cgaggaacag ccggagctac ggccggacta
      121 gacggcatca ggggacagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacgccggt ggcgaaagcg actgtctggg gtgtctgtga cgctgaggcg cgaaagccag
      241 gggatcaaac ggg
//
LOCUS       KHUT01009752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009752
VERSION     KHUT01009752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          101 g           43 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 aaagtctggg gtgaaatccg tcggcttaac cggcgaagtg cctcggatac tggcggtctc
      121 gagggaggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009753
VERSION     KHUT01009753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 gacgagggac cctagcgttg ttcggaatca ttgggcgtaa agcggaggta ggtggcttgg
       61 taagtcgagt gtgaaagccc agggctcaac cctggaagtg cattcgaaac tgctgagctt
      121 gagtgctaga gaggttacta gaatgcctgg tgtagtggtg aaatacgtag atatcaggcg
      181 gaataccgga ggcgaaggcg ggtaactggc tagacactga cactcaattc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009754
VERSION     KHUT01009754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           87 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcccgt
       61 taagtcagtg gtgaaatccc agggctcaac cctggaactg ccattgatac tgacgggctt
      121 gagttcggtt aaggcgggcg gaactggtgg tgtagcggtg aaatgcatag ataccaccaa
      181 gaaccccgat tgcgtaggca gctcgctgag ccgaaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009755
VERSION     KHUT01009755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggcggcg
       61 taagcggaat gtgaaagcct ggggctcaac cccagaagtg catcccgaac tgcgtcgctt
      121 gaatctcgga gggggacaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgtcctgga cgaagattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009756
VERSION     KHUT01009756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgta ggcggctatg
       61 taagtcgaat gtgaaatccc tgggctcaac ccgggaagtg cgttcgaaac tgcatagctt
      121 gagtcctgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg accacctgga cggagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009757
VERSION     KHUT01009757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           74 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattga
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaactg ccattgatac tgtcagtctt
      121 gagtatattt gaagtgggcg gaatgtgcag tgtagcggtg aaatgcttag atattgcaca
      181 gaacaccaat tgcgaaggca gctcactaaa ctataactga cgctgaggca cgaaagtatg
      241 gggatcaaac agg
//
LOCUS       KHUT01009758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009758
VERSION     KHUT01009758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggttcgg
       61 taagttggat gtgaaatctc ctggctcaac tgggagagga cgttcaagac tgtcggacta
      121 gaggacggta gaggaaggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctggg ccgttcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009759             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009759
VERSION     KHUT01009759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           92 g           51 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcaga
       61 ctaagtcaga tgtgaaattt atgggcttaa cccataacgg tcatttgata ctggtcgact
      121 agagtgtaga aggggttact ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg aggcgaaggc gggtaactgg gctaacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009760             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009760
VERSION     KHUT01009760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           92 g           51 t
ORIGIN      
        1 caccggcggc ccgagtggtg atcgtgatta ttgggtctaa agggtccgta gccggtttgg
       61 tcagtcctcc gggaaatctg acggctcaac cgttaggctt tcgggggata ctgccaggct
      121 tggaaccggg agaggtaaga ggtactacag gggtaggagt gaaatcttgt aatccctgtg
      181 agaccacctg tggcgaaggc gtcttaccag aacgggttcg acggtgaggg acgaaagctg
      241 ggggcacgaa ccgg
//
LOCUS       KHUT01009761             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009761
VERSION     KHUT01009761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           63 c          102 g           31 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta atcggaatca ttgggcttaa agggcgcgta ggcgggcgac
       61 cgcgtcgagt gctgaaagac cccggctcaa ccggggaacg ggcctcgaaa cgggtcgcct
      121 ggaggcgagg agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgccgc cggcgaaagc aaggtcctgg actcggcctg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01009762             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009762
VERSION     KHUT01009762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           84 g           52 t
ORIGIN      
        1 tacagaggga gcgagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtttag
       61 aaagtctact gtcaaatacc aaggctcaac cttgggcagg cagtagagta cttttagact
      121 agagtgtggt agaggtagag ggaatcccta gcgtaacagt gaaatgtgta gattttaggg
      181 agaacaccag cggcgaaggc gctctactgg gccaagactg acactgagag acgaaagcta
      241 ggggagcgaa tagg
//
LOCUS       KHUT01009763             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009763
VERSION     KHUT01009763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           86 a           35 c           75 g           58 t
ORIGIN      
        1 tacgagaggg gcgagcgtta ttcggaatga ttgggcgtaa agagcacgta ggcggtttat
       61 aaaatcctca ataaaaatct caaagctaaa ctttgaatgg agttgatgga tgcataaact
      121 agagtaagta agaggagaat ggaattcctg gagtagaggt aaaatttgtt gatctcagga
      181 ggaacaccga aagcgaaggc aattctctgc tacttaactg acgttgaggt gcgaaagcgt
      241 ggggatcaaa aggg
//
LOCUS       KHUT01009764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009764
VERSION     KHUT01009764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgaat
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tagctcgcta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009765             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009765
VERSION     KHUT01009765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          101 g           42 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gtaagtcgga agtgaaatgt cagtgctcaa cgctggcgct gcttccgata ctgcggatct
      121 ggggaccggt agaggccggt ggaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaacacccg tggcgaaggc ggccggctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009766             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009766
VERSION     KHUT01009766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           90 g           47 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggtctgt
       61 taagtcgttt gtgaaaatcc ggggctcaac cccgcgaaat gcatacgata ctggcagact
      121 agagactggt agaggctagc ggaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaacacctg tggcgaaggc ggctagctgg gccagatctg acactcatcc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01009767             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009767
VERSION     KHUT01009767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           85 g           56 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattga ttgggtgtaa agggtgcgta ggcggttttg
       61 aaagtcggat gtgaaatcct atggcttaac catagaattg cattcgaaac ttctagacta
      121 gagtgcgaaa gaggtaaagg gaattcccgg tgtaagggtg aaatctgtag agatcgggag
      181 gaacaccaga agcgaaggcg ctttactggt tcgcaactga cgctgagtgc acgaaagcat
      241 ggggagcaaa cggg
//
LOCUS       KHUT01009768             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009768
VERSION     KHUT01009768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           49 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcgg
       61 aaagtcagtt gtgaaatctc cgcgctcaac gcggaggggt cagctgaaac tgccgtgact
      121 tgagtctggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtactg acactgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009769             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009769
VERSION     KHUT01009769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           85 g           51 t
ORIGIN      
        1 tacgaggagc ccgagcgtta tccggattta ctgggcgtaa agggtgtgta gggggtctag
       61 ttagtcttgc cttaaatctt tgggctcaac ccaaagcctg ggcgagaaac ggctagactt
      121 ggagtatgca agaggcaagc ggaactcacg gtgtaggggt gaaatccgtt gatatcgtgg
      181 ggaacaccaa aggcgaaggc agcttgctgg ggcattactg accctgaaac acgaaagcgt
      241 ggggatcaaa aagg
//
LOCUS       KHUT01009770             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009770
VERSION     KHUT01009770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcttga
       61 taagccggat gtgaaacccc caagctcaac ttggggcggc cattcggaac tgtcatggct
      121 agagtctggt aggggaacac ggaattcctg gtgtagcggt gaaatgctta gatatcagga
      181 ggaacaccag tggcgaaggc ggtgttctgg gccagtactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01009771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009771
VERSION     KHUT01009771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtctgt
       61 cgcgtcggaa gtgaaaaccc ggagctcaac tccgggcctg ctttcgatac gggcagactt
      121 gagtgttgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caacaactga cgctgaggaa cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009772
VERSION     KHUT01009772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           93 g           40 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcagtcgtg
       61 taagtgcggt gtgaaatccc ccggcccaac cggggaactg ctccgcatac tgcacggcta
      121 gagcttggga ggggagagtg gaactcctgg tggagcggtg gaatgcgtag atatcaggag
      181 gaacgccgga ggtgaagacg actctctggc ccaatgctga cgctaaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009773
VERSION     KHUT01009773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctgt
       61 taggtcctgt gtgaaagccc cgggctcaac ccgggaagtg cgcaggatac cggcagactg
      121 gagtgtcgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga cgacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009774
VERSION     KHUT01009774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggtgggtaat
       61 taagtcatgg gtgaaaactg cgagctcaac ttgcagactg cctatgaaac tggttatctt
      121 gagggcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtccctga cactgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009775
VERSION     KHUT01009775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           90 g           51 t
ORIGIN      
        1 tacgtatggg gctagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggcttgt
       61 taagtcagat gtgaaatctg tgggctcaac ccgcagcctg catctgaaac tggcaggctt
      121 gagtctggta gaggatagtg gaattccagg tgtagcggtg aaatgcgcag atatctggag
      181 gaacaccagt ggcgaaggcg gctatctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01009776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009776
VERSION     KHUT01009776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtctca
       61 aaagtctact gtgaaatccc tgggcttaac ccaggacgtg cggtggatac tctgagactt
      121 gagtgccgga ggggtgcgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaga ggcgaaagcg gcgcactgga cggcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009777
VERSION     KHUT01009777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           95 g           52 t
ORIGIN      
        1 tacggggggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtctgtt gtgaaagccc cgggcctaac ctgggaagtg cactggatac tggcagactt
      121 gaatacggga gagggcagtg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009778
VERSION     KHUT01009778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          107 g           36 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgagca ggcggctgag
       61 agagcccggc gtgaaagccc ccggctcaac cgggggaggc cgtcggagac cttttagcta
      121 gaggccggca ggggtgagtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcactggg ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01009779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009779
VERSION     KHUT01009779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcgggctgc
       61 caagtcggat gtgaaagccc tcggcttaac cgaggaagtg cactcgaaac tggcagtctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009780
VERSION     KHUT01009780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c          100 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgag
       61 cgcgtccggg gtgaaagcct ccagcttaac tggagaagtg ccccggatac ggcccggctc
      121 gagcggggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcacgctgga cccctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009781
VERSION     KHUT01009781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtctgta ggtggctcgc
       61 taagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgagac tgatgagctc
      121 gagtgcagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctgcaactga cactgagaga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009782
VERSION     KHUT01009782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           99 g           39 t
ORIGIN      
        1 tacgaaggtg gcgagcgttg ttcggattca ctgggcataa agggcgcgta ggcggtgccg
       61 caagcagcgt gtgaaattcc ggggctcaac cccggaactg cgcgctggac tgcggtgctc
      121 gagtgcggga ggggggtgcg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacaccggt ggcgaaagcg acaccctggc ccgcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009783
VERSION     KHUT01009783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c          101 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgcgt
       61 caagtgtgat gtgaaagccc tgggctcaac ctgggaatgg catcacggac tgacgtgctg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctgtctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009784
VERSION     KHUT01009784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 gacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgtgta ggcggtttgt
       61 ttagtagggt gtgaaagcct ctggctcaac cagagaactg cgccctaaac tggcaagctg
      121 gagtgaggta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactgga ccttgtctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009785
VERSION     KHUT01009785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           85 g           48 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggcccgg
       61 taagttaggt gtgaaatgcc acagctcaac tgtggaattg cgcttaaaac taccgggctc
      121 gagggatata gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009786
VERSION     KHUT01009786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          102 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggg
       61 caagtcggcg gtgaaagccc ccagctcaac tggggaagtg ccctcgatac tgcttgtctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gcacgctggg cctttcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009787
VERSION     KHUT01009787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           94 g           43 t
ORIGIN      
        1 tacgaactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcctct
       61 caggtccgat gtgaaatccc ccggctcaac cggggaatgg cattggaaac cgacaggctg
      121 gagtgagcta gaggtgcatg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaggcg gtgcactggg gcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009788             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009788
VERSION     KHUT01009788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           90 a           31 c           65 g           70 t
ORIGIN      
        1 tacgggaagg gcaagcgtta tccggcgtga ctgggcgtaa agagtccgta gacggtaaag
       61 taagtttttt gttaaatttt aaaacttaat tttaagaaag caattaaaat actgctttac
      121 tttgagttta gtacagaaaa gtagaatttt atatgacagg ggtgaaatcc gtagatatat
      181 aaaggaatgc caacagcgaa ggcaactttt tagtataaac tgacgttgag ggacgaaagt
      241 gtgggtagca aacagg
//
LOCUS       KHUT01009789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009789
VERSION     KHUT01009789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           94 g           50 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgtgta ggcggccaga
       61 taagtagggt gtgaaaggcc ccggctcaac cggggcattg cgctctagac tgtctggctc
      121 gagtgagaca ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttgtctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009790
VERSION     KHUT01009790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggattta
       61 taagtcagtg gtgaaagccc cgagctcaac ttgggaattg ccattgatac tgtagatctt
      121 gagttcggtt gaggtaggcg gaatgttaca tgtagcggtg aaatgcttag atatgtaaca
      181 gaacaccgat tgcgaaggca gcttaccaag ccgttactga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009791
VERSION     KHUT01009791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           81 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggtatat
       61 taagtctgtt gttaaatgct ccagcctaac tggagacctg cagtggaaac tgatgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttagt ccatacctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009792
VERSION     KHUT01009792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatgtg
       61 taagtcagga gtgaaatccc agggcttaac cctggaactg ctcttgagac tgcatgtctg
      121 gaattcggta gaggttggcg gaactcccag tgtagaggtg aaattcgcag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccgacattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009793
VERSION     KHUT01009793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           83 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggaacaa
       61 taagtcagcg gtgaaagcct gcagcttaac tgcagaattg ccgttgatac tgttgttctt
      121 gagtacgttt gacgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agtgaagaca gctcacgaag acgaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009794
VERSION     KHUT01009794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           88 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggtttat
       61 caagacgaat gtgaaagccc cgggcttaac ctgggaactg catgcgtgac tgataagcta
      121 gagtatggca gagggaggca gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaatatcgat ggcgaaggca gcctcctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009795
VERSION     KHUT01009795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           86 g           58 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 taagtctggt gtgaaatctc ggggctcaac cccgaaattg cattggatac tatattgcta
      121 gaggattgta gaggagagtg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt tgcgaaggcg actctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009796
VERSION     KHUT01009796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           48 c          101 g           52 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggctcgt
       61 cgcgtctgcc gtgaaagact acagcttaac tgtggttttg cggtggatac gggcgggctt
      121 gaggccagta ggggagatcg gaattctacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gatctctggg ctggttctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01009797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009797
VERSION     KHUT01009797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           90 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtctgt
       61 caagtcaggg gtgaaatcct tcggcttaac cggagaacag cccctgatac tgatgggctt
      121 gagggagata gaggcagccg gaactaccgg tggagcggtg aaatgcgttg atatcggtag
      181 gaacgccaga ggcgtaagcg ggctgctgga tctcttctga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009798
VERSION     KHUT01009798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           97 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggtgtaa agggcgcgta ggcggcatga
       61 tgtgtgtgat gtgaaagccc cgggcttaac ctgggaagtg cattgcagac tatcgagcta
      121 gagtatgtga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc acaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009799
VERSION     KHUT01009799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           94 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttggg
       61 taagtttggt gtgaaatctc ctggcttaac tgggagggtg cgccgaaaac tgcctggctt
      121 gagtgcggga ggggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctctctgga ccgaaactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009800
VERSION     KHUT01009800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgtgcgta ggcggccctt
       61 caagtagaat gtgaaatccc tgggctcaac ccaggaagtg cattctagac tgattggctt
      121 gaatcctgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccagt ggcgaaggcg accacctgga cggagattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009801
VERSION     KHUT01009801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtatt
       61 caagtccggg gtgaaagccc ccggctcaac cggggaattg ccttggatac tggatgtcta
      121 gagcgaggta ggggccgttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aacggctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009802
VERSION     KHUT01009802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c          101 g           42 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggatggg
       61 taagtgggtg gtgaaagcct ctggctaaac taggggagga ccattcagac tgccgatcta
      121 gaggcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009803             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009803
VERSION     KHUT01009803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           81 g           55 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gacggtctag
       61 taagttagca gtgggaaatc gaggcccaac ctcgtggaag ttgctaatac tactatactc
      121 gagtgcagga gaggttaacg gaactctacg tggagcggta aaatgtgacg atatgtggat
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacgttgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01009804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009804
VERSION     KHUT01009804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttat
       61 taagatggat gtgaaatccc tgggcttaac ctaggaactg cattcatgac tggtaaactg
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009805
VERSION     KHUT01009805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           90 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggcctgg
       61 taagtcgact gtgaaagccc tcggctcaac cgaggaagtg cagtcgaaac tgctaggcta
      121 gagtgtcgaa gaggatggcg gaatgcgcag tgtagaggtg aaattcgtag atattgcgta
      181 gaacaccagt ggcgaaggcg gccatctggg agacaactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009806
VERSION     KHUT01009806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           91 g           50 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgcgta ggcggtttag
       61 tgtgtgggaa ttgaaagcct ggggctcaac tccagacccg gttcccaaac tgctagactt
      121 gagcgtatca gaggacagcg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgtctggg gtattgctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009807
VERSION     KHUT01009807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           84 g           51 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttac agggtgcgta ggctgtgcag
       61 caagtgaggt gtgaaatcct acggcttaac cgtagaactg cgcttcaaac tgctgtgctc
      121 gagggagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009808
VERSION     KHUT01009808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           92 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcttcg
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaattg cgcttgaaac tacggtgctg
      121 gagtgcggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccttctggg tcgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009809
VERSION     KHUT01009809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           49 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggcggtgtag
       61 caagttgggg gtgcaagctc tgggcttaac ccagaaactg cctccaaaac tactatgctt
      121 gagagtcaaa ggggaagctg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaggcg ggcttctggt tgacttctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009810
VERSION     KHUT01009810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           82 g           53 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatata
       61 caagtcagaa gtgaaatccc agggcttaac cctggaattg cttttgagac tgtatgtcta
      121 gaaaccagaa gaggttagcg gaatatccag tgtagaggtg aaattcgcag atattggata
      181 gaacaccagt ggcgaaggcg gctaactggt ctggtattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009811
VERSION     KHUT01009811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgc
       61 taagttatct gtaaaatccc tgggcttaac ctgggcaggt cagataagac tggtagactc
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctgtctggc ccaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009812
VERSION     KHUT01009812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           96 g           39 t
ORIGIN      
        1 tacgaactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggcccgc
       61 agggtccgat gtgaaatccc ccggctcaac cggggaacgg cattggaaac cggcgggctg
      121 gagcgagcta gaggtgcatg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg atgcactggg gcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009813
VERSION     KHUT01009813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          103 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggcctgg
       61 taagtcaggg gtgaaagacc ggggctcaac cccgggggtg cccatgaaac tgctgggctt
      121 gagtgctcca gagggcagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg gagtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009814
VERSION     KHUT01009814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccta
       61 aaagtctggt gtgaaaactt gaggctcaac ctcaagctgt cgctggatac tgtagggctt
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gcactctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009815
VERSION     KHUT01009815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           85 g           56 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggatata
       61 caagttagat gtgaaatacc tgggcttaac ctgggaatgg catttaagac tgtatgtcta
      121 gagtttgtca gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgcaggca gccccctggg ataaaactga cgctgtggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009816
VERSION     KHUT01009816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           40 c           88 g           59 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtctgat gttaaagact ggggctcaac ctcggaaatg cattggatac tggcagactt
      121 gagtgtggta gaggcaatcg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgcaagcg gattgctggg ccataactga cactgaggaa cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01009817             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009817
VERSION     KHUT01009817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           42 c           79 g           56 t
ORIGIN      
        1 gacataggtc gcgaacgtta tccggaatca ttgggcgtaa agggtgcgta gatggtgtga
       61 taagttattg gtggtaaacc gaggctcaac ctcgtggaag ctgataatac tgtcgcacta
      121 gagtgtagga gaggttaacg gaactccatg tggagcggta aaatgcgtag atatatggaa
      181 gaacatcaat aacggcgaag gcagttaact attctactac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009818
VERSION     KHUT01009818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtcaga
       61 taagtcgggt gtgaaagccc tgggcttaac ctgggaactg cattcgatac tgtctgacta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009819
VERSION     KHUT01009819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggattcg
       61 tatgtcggat gtgaaatccc caggcttaac ctgggaattg catccgaaac tgcgaacctt
      121 gaatgcttga gaggatgacg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gttgtctggc gatgcattga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009820
VERSION     KHUT01009820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           84 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcacgta ggtggatact
       61 taagtcgaat gtgaaatccc tgggctcaac ctaggaactg catacgatac tgggtgtcta
      121 gagtatagta gagggaagtg gaatttccgg agtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctaatactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009821             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009821
VERSION     KHUT01009821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           84 g           56 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcttga
       61 taagttagta gtgggaaatc gaggctcaac ctcgtggaag ctggtaatac tgtcaggctc
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgtgtag atatatggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009822
VERSION     KHUT01009822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggcaatt
       61 caagtctgat gtgaaagccc tgggcttaac ctgggaattg cgttgggaac tgaatagcta
      121 gagtcggaca gagggccgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacacccgt ggcgaaggcg gcggcctggg tccagactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009823
VERSION     KHUT01009823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 gacagaggag gcaagcgttg ttcggaatca ttgggcttaa agcgcgtgta ggcggcccgt
       61 caagtgcctt gtgaaatccc acggctcaac cgtggaactg cttggtagac tggcgggctt
      121 gaggctggta gaggtgcctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacacctga ggcgaaagcg gggcactggg ccggttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009824
VERSION     KHUT01009824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtccggg gtgaaatcct ccagcttaac tggagaactg cctcggatac tggaggtctt
      121 gaggaagata ggggcatgcg gaaccgtggg tggagcggtg aaatgcgttg atatccatgg
      181 gaactccggt ggcgaaggcg gcatgctgga tcttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009825
VERSION     KHUT01009825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggagtta ttgggcgtaa agagcgcgta ggtggttcga
       61 taagtttggt gtgaaatccc tggactcaat ccaggaagtg cattgaatac tgtcggacta
      121 gagtacagga gaggtaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gcttactgga ctgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009826             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009826
VERSION     KHUT01009826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           39 c           79 g           60 t
ORIGIN      
        1 gacgtaggtc gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtttaa
       61 taagttatca gcggtaaatc ggggctcaac ctcgtggaag tcggtaatac tgttatactt
      121 gagtgcagga gaggttaacg gaactccatg tggagcggta aaatgcgtag atatatggaa
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01009827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009827
VERSION     KHUT01009827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           76 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggatagg
       61 taagtcagtg gtgaaatccc atcgcttaac gatggaactg ccattgatac tgcttatcta
      121 gagtacagtt gccgttggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaag ctgtaactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01009828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009828
VERSION     KHUT01009828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           95 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcccac
       61 taagtcacac gtgaaatccc tcagcttaac tggggaactg cgtgtgagac tggagggctt
      121 gagtgcagga gaggaatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcattctgga ctgcgtctga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009829
VERSION     KHUT01009829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           96 g           43 t
ORIGIN      
        1 aacgtaggac acgagcgtta tccggagtta ctgggcgtaa agggcgggta ggtggcaatg
       61 taaggttggt gtgaaagcct tcggctcaac cgagggagga cactgacgac tgcatagctc
      121 gaggcagata gaggggtgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcaccctggg tctgacctga cactgatccg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009830
VERSION     KHUT01009830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggattca ctgggcttac agggtgcgta ggcggcctgt
       61 taagtagggt gtgaaagccc ccggctcaac cggggaatgg cgccctaaac tggcgggctg
      121 gagtgagata agggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009831
VERSION     KHUT01009831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgaag
       61 aaagtcggat gtgaaatttg agggctcaac cctcaactag catctgatac tactttgcta
      121 gagaactgga gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag atctcgagag
      181 gaacgctcgc tgcgaaagcg aagctctggc cagtttctga cgctgaggca cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009832
VERSION     KHUT01009832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           85 g           48 t
ORIGIN      
        1 gacgagcctc acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaatag cgctcaaaac taccatgctc
      121 gagggagata gaggaatgcg gaactcaggg tggagcggtg aaatgcgttg atatcctggg
      181 gaacaccggt ggcgaaagcg gcattctgga tctcatctga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009833
VERSION     KHUT01009833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggctggccag
       61 caagtcagat gtgaaagccc tgggctcaac ccaggaactg catttgaaac tgctggtctt
      121 gaatagtgga gagggtcgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgacctgga cacttattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009834
VERSION     KHUT01009834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agggcatgta ggcggtttgc
       61 taagtccgcg gttaaagccc ccggctcaac tgggggaggt ccgtggagac tatcagactt
      121 gagggcaaca gaggcacgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg acgtgctggg ttgcgactga cgctgaaatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009835
VERSION     KHUT01009835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           50 c           81 g           48 t
ORIGIN      
        1 gacagagcat gcaagcgcta tccagaatga ttgggcgtaa agcgtctgta ggtgtctttt
       61 caagtccgcc gtaaaatccc tgggctcaac cctggacagg cggtggatac taccaagctg
      121 gagtacggta ggggctgagg gaatttcggg tggagcggtg aaatgcattg agatcagaaa
      181 gaacaccaat ggcgaaagca ctctgctggg ttgacactaa cactgagaga cgaaagctag
      241 aggagaaaat ggg
//
LOCUS       KHUT01009836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009836
VERSION     KHUT01009836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggtggtggtt
       61 taagtctgat gtgaaagccc acggctcaac cgtggaaggt cattggaaac tggatcactt
      121 gagtgcagga gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009837
VERSION     KHUT01009837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           84 g           53 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtgatg
       61 caagtcggtt gtgaaattcc ggagcctaac tccggaactg cattcgaaac tgcattgctc
      121 gaggtctgga gaggtaattg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaagcg aattactgga cagaacctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009838
VERSION     KHUT01009838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           95 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcatgta ggcgggatgt
       61 taagtcgcgt gtgaaagccc caggctcaac ctgggaagtg cgcgcgaaac tggcaacctt
      121 gagtgctgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cagacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009839             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009839
VERSION     KHUT01009839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           36 c           80 g           61 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcataa
       61 taagttattg gtgggaaatc gaggctcaac ctcgtggaag ttagtaatac tgttttgctt
      121 gagtacagga gaggttaacg gaactttctg tggaggggta aaatccaaag atatagacgg
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01009840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009840
VERSION     KHUT01009840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           82 g           59 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcttg
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgaggtg ccattgatac tgcaagactt
      121 gagtacagac gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttactaga ctgtaactga cactgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009841
VERSION     KHUT01009841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggt
       61 taagtctgat gtgaaatctc caagctcaac ttggaaatgg catcggaaac tatccggcta
      121 gaggagtgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cacttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009842
VERSION     KHUT01009842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgtgtgta ggcggcctgt
       61 taagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cattcgaaac tggcaggctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009843
VERSION     KHUT01009843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           84 g           57 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggataga
       61 taagtcagat gtgaaattcc aaggctcaac tttggagctg catttgaaac tgtttatctt
      121 gagggatggc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacacctgt ggcgaaagcg gttttctagc ttatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009844
VERSION     KHUT01009844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           90 g           53 t
ORIGIN      
        1 tacagggggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcgggctgt
       61 taagtccggt gtgaaatccc tcggctcaac tgaggacgtg cactggatac tggcagtctt
      121 gagtcctgga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cagtgactga cgctgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009845
VERSION     KHUT01009845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           77 g           60 t
ORIGIN      
        1 tacgaaggat cctagcgttg tccggattta ctgggtttaa agggtgcgta ggcggataat
       61 taagtcagtg gtgaaagccg gcagctcaac tgtcgaattg ccattgaaac tgattatctc
      121 gagtatggtt gaggtggttg gaatgttgca tgtagcggtg aaatgcttag atatgcaaca
      181 gaacaccgat tgcgaaggca gaccactaag ccattactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009846
VERSION     KHUT01009846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c          101 g           42 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggagtta ctgggcgtaa agggtgtgta ggcggtgcta
       61 taagtggggt gtgaaacact caggctaaac ctgggaggtg cgcctcagac tgtaggacta
      121 gagtgggata gaggggcgtg gaattccggg tggagcggtg aaatgtgtag agatccggag
      181 gaacaccaga ggcgcaagcg gcgccctggg tcccaactga cgctgaaaca cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009847
VERSION     KHUT01009847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggaagat
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaactg catcttaaac tgattttcta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009848
VERSION     KHUT01009848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           64 c           98 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 cgagtccttg gtgaaagccc ccagctcaac tggggaagcg ccagggatac tgccggcctc
      121 gagcggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009849
VERSION     KHUT01009849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           83 g           54 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggcggtttga
       61 taagtctgtt gtgtcagctc ctggcttaac tgggagaggt caacggaaac tatcaggctt
      121 gagtgtagga gaggatactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaagca agtatctgga ctattactga cgctaagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009850
VERSION     KHUT01009850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           99 g           43 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgtgg
       61 caagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cattcgatac tgccgcgctg
      121 gagtctcgga gaggatggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009851
VERSION     KHUT01009851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggtttga
       61 taagtcctgt gtgaaagccc ccggctcaac cggggaactg cactggatac tgtcaggcta
      121 gagtgtagaa gaggagagcg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccagt ggcgaaagcg gctctctggt ctactactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009852
VERSION     KHUT01009852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           92 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggagtcta ggcggttcgg
       61 taagtgggat gtgaaatccc tcggctcacc cgaggacctg catcccatac tgctgagctt
      121 gagtacagga gagggtgggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cccacctggc ctgctactga cgctaaagct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009853
VERSION     KHUT01009853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggcggctttg
       61 caagtcaggg gtgaaatccc acagctcaac tgtggaattg cctttgaaac tgcatggctg
      121 gagtgtcgga ggggatagcg gaattcctaa tgtagtggtg aaatacgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009854
VERSION     KHUT01009854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           83 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggaaagt
       61 taagtcagat gtgaaattcc gaagctcaac atcggagctg catttgaaac tagctttctt
      121 gaggattggc ggagagaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttctctagc taaatccaga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009855
VERSION     KHUT01009855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccaac
       61 taagtcgaac gtgaaatccc caggctcaac ctgggaactg cgtccgagac tgattggctc
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009856
VERSION     KHUT01009856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           93 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggtctcg
       61 caagtcgagt gtgaaagccc cgggcttaac ctgggaattg cgctcgaaac tacgggactg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cactcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009857
VERSION     KHUT01009857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           87 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggcggtcaag
       61 caagtctatt gtgaaatccc ggggctcaac cccggaactg caatggaaac tacttggctg
      121 gagcaatgta ggggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccaat ggcgaaggca cctcactgga catttgctga cgctgagctg cgaaagtagg
      241 gggagcaaac agg
//
LOCUS       KHUT01009858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009858
VERSION     KHUT01009858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttcgc
       61 caagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tagtggactc
      121 gagtgtggga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgcagacg gcttcctgga ccaccactga cgctgaaaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009859
VERSION     KHUT01009859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcgggtttg
       61 taagtcgatt gtgaaatccc tgggctcaac ctgggaatgg cagtcgaaac tgcaaatctt
      121 gaatgtctga gaggatggcg gaattcccgg tgtagtagtg aaatacgtag atatcgggag
      181 gaacacctga ggcgaaggcg gctatctggc ggtacattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009860
VERSION     KHUT01009860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           88 g           56 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtcagt
       61 taagtcggat gtgaaatccc taggctcaac caaggaagtg cattcgaaac tagctggctg
      121 gaagacggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ctgttcttga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009861
VERSION     KHUT01009861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttat
       61 caagtcgggg gtgaaatcca gcagcttaac tgttgaagtg ccttcgatac tgatgaacta
      121 gaggaaggta ggggtgtgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acgcactgga ccttttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009862
VERSION     KHUT01009862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggccgccctg
       61 caagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac tgcagggctt
      121 gagtcctgga gaggatagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gctatctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009863
VERSION     KHUT01009863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agcgcgcgca ggcggccatc
       61 tacgtctggg gtgaaagccc aaggctcaac cttgggattg ccttggatac gggatggctt
      121 gaggatcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009864
VERSION     KHUT01009864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           99 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggaatta ctgggcgtac agggcacgca ggtggctgca
       61 taggtggcgt gtgaaagccc cgcgcttaac gcggggaggc cacgccaaac ggtgcggctc
      121 gagcgatgca gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag gacgaaggta accacctggg catcagctga cactcatgtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009865
VERSION     KHUT01009865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgtt
       61 ccagtctggg gtgaaatcct ctcgctcaac gagagaactg ccctggatac tgaacacctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009866
VERSION     KHUT01009866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggttat
       61 taagtcagat gtgaaattcc agggctcaac cctggagctg catctgaaac tggtaatctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagagtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009867
VERSION     KHUT01009867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttacg
       61 taagttaggt gtgaaattcc tgggcttaac ctggggactg cgcctgatac tgcgtagcta
      121 gagtcttgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga caaagactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009868             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009868
VERSION     KHUT01009868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT          103 a           30 c           56 g           66 t
ORIGIN      
        1 tacagagggt gcgagcatta ttcgaaataa ctgggcgtaa agagcaagta gatggcttat
       61 taaagttaat agtaaaatcc caaaaaactt ttttggacaa gctattaaaa cgaataggat
      121 agagtttcat agaggatagt ggaattttta atgtagagtt aaaattcatt aatattaaaa
      181 ggaacaccaa aggcgaaagc aactatctaa atgaatactg acattgagaa tgcgaaagct
      241 tgggtatcaa acggg
//
LOCUS       KHUT01009869             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009869
VERSION     KHUT01009869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           37 c           79 g           66 t
ORIGIN      
        1 tacgagaggt gcaagcgttg tccggattta ttgggcgtaa agcgtttcta aaggcgcttt
       61 tgtaagttgt ttttcaaaga ccgaagctca acttcgggaa gggagacaat actgcaagag
      121 ttgaaatatt tcggggttac tagaactatc ggtgtagggg tgaaatccgt tgatatcgat
      181 aggaattcca agggcgtagg caggtaacta ggaatttttt gacgctgatg aacgaaagct
      241 aggggagcga aaggg
//
LOCUS       KHUT01009870             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009870
VERSION     KHUT01009870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           78 g           60 t
ORIGIN      
        1 gacataggtc gcaaacgtta ttcggaatta ttgggcgtaa aggatgcgta gatggttcgg
       61 taagttactg gtgggaaatc gaggcccaac ctcgtggaag ttagtaatac tgccgaactt
      121 gagtgcagga gaggttaacg gaactttatg tggagcggta aaatgcgtag atatatgaaa
      181 gaacatcaat atagcgaagg cagttaacta ttctgctact gacgttgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01009871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009871
VERSION     KHUT01009871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtga ctgggcgtaa agggcatgta ggcggttatg
       61 ttagtttgat gtaaaatacc cgggcttaac ccgggacctg cattagaaac ggcatagcta
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacactcgt ggcgaaggcg gctttctggc ccataactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009872
VERSION     KHUT01009872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttagt
       61 taagttagtt gtgaaatccc tgggcttaac ctaggaactg caactgatac tggctagcta
      121 gagtgtagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctaacactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009873
VERSION     KHUT01009873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           80 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcgggcctt
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tggaggcctt
      121 gagtttcgga gaggaagacg gaactgcagg tgtagcggtg aaatgcgttg atatctgcag
      181 gaacaccaat ggcgaaagca gtcttctgga cgaatactga cgctcaggtt cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01009874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009874
VERSION     KHUT01009874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           92 g           51 t
ORIGIN      
        1 gacgaactgc ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatg
       61 caagtcaggg gtgaaatccg gcagcttaac tgtcgaagtg cctttgatac tgcatgtctt
      121 gagggaggta ggggtccgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tcttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009875
VERSION     KHUT01009875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agagtatgta ggcggccttg
       61 caagtcaggt gtgaaatccc acggctcaac cgtggaattg catttgaaac tgcagggctt
      121 gaatatggaa gagggcagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gctgcctggt ccaatattga cgctgagata cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009876
VERSION     KHUT01009876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcgagg
       61 gaagtcaggt gtgaaattcc ggggctcaac tccggagctg cacttgagac ttttttgcta
      121 gagttcagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gttttctggg ctgacactga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009877
VERSION     KHUT01009877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaataa ctgggcgtaa agggcgcgta ggcggttaat
       61 caagtgagag gtgaaatccc cgggcttaac ctgggaacag cctttgagac tggttagctg
      121 gagtacagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg accacctgga ctgacactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009878
VERSION     KHUT01009878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggcgtaa agcgttcgta ggcggtgcag
       61 taagttgggt ttgaaagccc ggggctcaac tccggacccg gatccaaaac tactgcgctg
      121 gagcatatca gggggcagcg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg gtattgctga cgctgaggaa cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009879
VERSION     KHUT01009879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           82 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca gacggtttgc
       61 taagtcaact gttaaattcc ccggcctaac cgggagctcg cagtagaaac tgacaaacta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009880
VERSION     KHUT01009880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttttc
       61 caagttggag gtgaaatccc cgggcttaac ctgggaactg cctccaagac tgggagactc
      121 gagtccgaga gaggtgagtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggag cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009881
VERSION     KHUT01009881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           85 g           53 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggtgcgta ggcggttagg
       61 aaagtcagat gtgaaattcc ggggctcaac ctcggagctg catttgaaac ttccagacta
      121 gagttcaatt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggtgaagacg gttttctggg ttgatactga cgctgaggca cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009882
VERSION     KHUT01009882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           86 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggattta ctgggcgtaa agcgtacgca gacggtgagg
       61 caagtcgggt gtgaaagccc cgggctcaac ccgggaactg cattcgatac tacttcgctc
      121 gagtatgaga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gctttctggc tcattactga cgttcaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009883
VERSION     KHUT01009883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           40 c           77 g           55 t
ORIGIN      
        1 gacgtaggat gcaagcgtta tccggaatta ctgggcgtaa agagttcgta ggcgttttgt
       61 taagtagaat gttaaagact tgagctcaac tcagggaagg cattctaaac tggcagaata
      121 gagtaagaca gaggcaagtg gaattctgtg tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gcttgctggg tctttactga cgctcaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009884
VERSION     KHUT01009884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacagagggt gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctcgg
       61 tatgtcgggt gtgaaagccc acctcttaaa ggtggaattg cattcgaaac taccgagcta
      121 gagtatcgga gaggatggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccatctgga cgaatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009885
VERSION     KHUT01009885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          104 g           36 t
ORIGIN      
        1 aacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggcggcccct
       61 taagtccggt gtgaaagccc gcggctcaac cgcgggaggc cactggaaac tggggggctt
      121 gagggcagga gaggggagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctccctggc ctgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009886
VERSION     KHUT01009886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           85 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggcaagg
       61 tcagtcagat gtgaaatcct agggcttaac cctagaatgg catccgatac taccttgcta
      121 gagtagtgga gaggagtctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg agactctgga cacttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009887
VERSION     KHUT01009887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggccaag
       61 taagtaggag gtgaaatctc atggctcaac tgtgaggctg ccttctagac tgctaggctt
      121 gagcatggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccattgctga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009888
VERSION     KHUT01009888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           84 g           49 t
ORIGIN      
        1 gacgtaccac ccaaacgtta ttcggattca ctgggcttac agagtccgta ggcggtcaag
       61 aaagttaggc gtgaaatccc tcagctcaac tgaggaatag cgcttaaaac tacttggcta
      121 gagagggtca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg gcttttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009889
VERSION     KHUT01009889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggtcagg
       61 taagtcgggc gtgaaatccc actgcttaac agtggaactg cgttcgatac tgcttggcta
      121 gaggattgga ggggggatcg gaatttatgg tgtagcagtg aaatgcgtag atatcataag
      181 gaacaccggt ggcgaaggcg gatccctgga aaattcctga ctctgaaaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009890
VERSION     KHUT01009890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           42 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgctc
       61 taagtcccgt gtgaaagccc ccggctcaac tggggaactg cacgggaaac tggagtgctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgagagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009891
VERSION     KHUT01009891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggcctgt
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaagtg cattcgatac tgataggctc
      121 gagtaccgga gagggtcgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gcgacctgga cgagtactga cgctcagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009892
VERSION     KHUT01009892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggttttg
       61 taagtcgggc gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgcggaacta
      121 gagtttcgga gaggttggta gaattcttgg tgtagaggtg aaattcgtag atatcaagag
      181 gaataccggt ggcgaaggcg gccaactgga cgaaaactga cgctgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01009893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009893
VERSION     KHUT01009893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggccttt
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg cgtttgaaac tggaaggcta
      121 gagtgtggaa gaggggggtg gaattccagg tgtagcagtg aaatgcgtag agatctggag
      181 gaacaccgat ggcgaaggca gccccctggg ctgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009894
VERSION     KHUT01009894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctct
       61 caagtcaggg gtgaaatctt tcggcccaac cggaaaaggg cccctgatac tgagagtctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009895
VERSION     KHUT01009895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggtgcgta ggcgggcggc
       61 caagtcgggg gtgaaatctt tcagctcaac tggaaaacag ccttcgatac tggtcgtctg
      121 gaggaaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009896
VERSION     KHUT01009896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           84 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggccgcg
       61 taagtcagat gtgaaatcct ggagcttaac tccagaacag catttgaaac tgtgcggcta
      121 gagggtagtc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacatctgt ggcgaaggcg gttttctaga ttactcctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01009897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009897
VERSION     KHUT01009897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           83 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtgact
       61 taagtcagtg gtgaaatact tcagcttaac tggagaggtg ccattgatac tgattcactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009898
VERSION     KHUT01009898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtctaa
       61 taagtctggt gtgaaatccc tggactcaat ccaggaagtg cattggatac tgttagactg
      121 gagtacagga taggaaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctttctgga ctgtcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009899
VERSION     KHUT01009899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggatggg
       61 taagtcaggg gtgaaatctc acagctcaac tgtgaaactg cctttgatac tgcccatctt
      121 gagtacggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc ccggaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009900
VERSION     KHUT01009900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          111 g           36 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggatcca
       61 cgcatcctcg gtgaaagccc cccgctcaac gggggagggt ccggggagat ggtgggtctg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gctggctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009901
VERSION     KHUT01009901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           90 g           45 t
ORIGIN      
        1 tacagggggc gcgagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcggaccgt
       61 taagtcggat gtgaaatccc agggctcaac cctggaagtg cattcgatac tggcggtctc
      121 gagtacggga gaggagagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctctctggc ccgatactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009902
VERSION     KHUT01009902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           99 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccag
       61 caagtctggg gtgaaatctc ccggctcaac cgggaaattg cctgggatac tgctggcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccggt ggcgaaagcg gcacgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009903
VERSION     KHUT01009903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           87 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggccgaccgg
       61 aaagttgggt gtgaaagccc tgggctcaac ccaggaactg cactcaaaac taccggtctc
      121 gagtgatgga gagggaagtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gcttcctgga catcaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009904
VERSION     KHUT01009904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttacg
       61 taagttaggt gtgaaagccc cgggctcaac ctgggaattg cgcttaatac tgtgtagcta
      121 gagtgttgta gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttactgga caataactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009905
VERSION     KHUT01009905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 taagtcaggc gtgaaaggcc cgggctcaac ctggggactg cgcttgatac tgcgaggctt
      121 gaatccggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009906
VERSION     KHUT01009906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           94 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtattt
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaattg cattttagac tgaggtactg
      121 gagtacagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg accacctgga ctgaaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009907
VERSION     KHUT01009907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggttata
       61 caagtcaggg gtgaaatccc ggagcttaac tccggaactg cctttgaaac tgtatagctg
      121 gaggatgtga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctggc acatttctga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009908
VERSION     KHUT01009908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           99 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggccatg
       61 caagtctgac gtgaaagccc agggctcagc cctggaagtg cgttagaaac tgcggggctt
      121 gagtaccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggtcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009909
VERSION     KHUT01009909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           96 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggtgaga
       61 tgtgagtgat gtgaaagccc cgggcttaac ctgggaaggg catcgctaac tatctgactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttaatactga cactgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009910
VERSION     KHUT01009910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggtggtctgc
       61 caagtccggg gtgaaatcct ccagctcaac tggagaagtg ccttggatac tggcggactc
      121 gagggaggca ggggtcgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacgactggt cctcttctga cactgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU9999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009911
VERSION     KHUT01009911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU9999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta gatggttatg
       61 taagtcggat gtgaaagccc agggctcaac cttggaattg cattcgagac tgcctagcta
      121 ggatgcggaa gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcttcctggt ccagcatcga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009912
VERSION     KHUT01009912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c          101 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcacc
       61 aaagtccggg gtgaaagccc ccagctcaac tggggaaggg cctcggatac tgggtgcctc
      121 gagggaggta ggggcgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga cctctgctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009913
VERSION     KHUT01009913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           86 g           47 t
ORIGIN      
        1 gacggaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 aaagttaggt gtgaaagccc tcggctcaac cgaggaattg cgcccaaaac tactgtgcta
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009914
VERSION     KHUT01009914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           42 c           73 g           59 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggactta
       61 taagtcagtg gtgaaagccg atagcttaac tatcgaactg ccattgatac tgtaggtcta
      121 gagtatagat gatgttggcg gaatatgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccgat tgcgaaggca gctgacaaaa ctataactga cgctgatgca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01009915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009915
VERSION     KHUT01009915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcagctgag
       61 caagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac tgctgagcta
      121 gagttctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cagcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009916
VERSION     KHUT01009916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           89 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgat
       61 taagttatct gtaaaatccc tgggcttaac ctgggagtgc cagatgatac tgattgactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009917
VERSION     KHUT01009917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgga
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaacag cctttgatac tgtccgtcta
      121 gagggaggta ggggtgcgtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg acgcactgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009918             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009918
VERSION     KHUT01009918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           41 c           78 g           57 t
ORIGIN      
        1 gacataggtc gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtatga
       61 taagttagcg gtgggaaatc gaggctcaac ctcgtggaag ctgataatac tgttgtactt
      121 gagtacagaa gaggttaacg gaactccatg tggagcggta aaatgcatcg atatatggaa
      181 gaacaccaat aacggcgaag gcagttaact attctgttac tgacgttgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009919             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009919
VERSION     KHUT01009919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           49 c           79 g           53 t
ORIGIN      
        1 tacagagacc tcaaacgtta tccggaatca ctgggcgtaa agcgtaccga tcggtggttt
       61 tgtaagtctt tggtgaaagc ctggagctca actccagaat cgccaaggaa actataagac
      121 tagaagtgca aagagggagc tggaactatg ggtgtagcgg tgaaatgcgt tgatatccat
      181 aggaacacta atagcgaagg caggctcctg ggtgcatctt gacactgcta ggacgaaagc
      241 gtggggagcg aatggg
//
LOCUS       KHUT01009920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009920
VERSION     KHUT01009920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttaa
       61 taagtccgtg gtgaaagccc accgctcaac ggtggaactg ccatggatac tgttaggctt
      121 gagtacagac gaggtaggcg gaatggatcg tgtagcggtg aaatgcatag atacgatcca
      181 gaacaccgat tgcgtaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009921             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009921
VERSION     KHUT01009921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           40 c           78 g           57 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcgcga
       61 taagttatca gtggcaaatc agggctcaac cctgtggaag ctggtaatac tgtcatgcta
      121 gagtacagaa gaggttaacg gaactccatg tggagcggta aaatgtgtag atatatggat
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01009922             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009922
VERSION     KHUT01009922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           45 c           81 g           54 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggcctgg
       61 caagttatca gtggtaaatc gaggcccaac ctcgtggaaa ctggcaatac tgcttggctt
      121 gagtgcagga gaggttaacg gaactctatg tggagcggta aaatgcacag atatatagaa
      181 gaacatcaat aacggcgaag gcagttaact attctgttac tgacactgag gcacgaaagc
      241 gtggggagca aaacgg
//
LOCUS       KHUT01009923             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009923
VERSION     KHUT01009923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           49 t
ORIGIN      
        1 cacgtaaggg gcaagcgttg ttcggaatta ttgggcgtaa agggcatgca ggcggtcggg
       61 aaagtccggt gtgaaaggtt ggagctcaac tccaaaatcg cgctggaaac tgctctgact
      121 cttgagtccc ggagaggaag ctagaattcc aggtgtaggg gtgaaatctg tagatatctg
      181 gaagaatacc gatggcgaag gcaagcttct ggccgaggac tgacgctgag gtgcgaaagc
      241 gtggggagca aacagg
//
LOCUS       KHUT01009924             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009924
VERSION     KHUT01009924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           83 g           57 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatta ttgggcgtaa agcgtgccga taggtggttt
       61 ggttagtctt gggtgaaatc ccgaagctca acttcgggtt tgcttaagat actgccagac
      121 tagaggggca aagaggagat tggaacgaac ggtgtagtag tgaaatgcgt tgatatcgtt
      181 cggaacaccg aaagcgaagg cagatctctg ggtgccacct gacactgcta ggacgaaagc
      241 gtgggtagcg aatggg
//
LOCUS       KHUT01009925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009925
VERSION     KHUT01009925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           85 g           56 t
ORIGIN      
        1 tacagagagt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatgct
       61 taagtcggat gtgaaagccc tgggcttaac ctaggaattg cattcgatac tgggcttcta
      121 gagtatagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcttcctgga ctaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009926
VERSION     KHUT01009926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttagt
       61 taagtgaggt gtgaaagtcc tgggcttaac ctgggaaagt catttcatac tgattgacta
      121 gagtaggata gagggaggtg gaatttccgg cgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gcctcctgga tccatactga cactgaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009927
VERSION     KHUT01009927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           96 g           50 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgatg
       61 caagtcaggt gtgaaagccc cgggcttaac tcgggaattg ctcctgaaac tgcattgctt
      121 gagtcctgga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgt ggcgaaggcg gctacctgga cagtgactga cgctgatgcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009928
VERSION     KHUT01009928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           75 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggcttaa agggtgcgta ggtgggcagt
       61 taagtcagtg gtgaaatctt cccgcttaac gggaaaactg ccattgatac tatctgtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cgaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009929
VERSION     KHUT01009929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           87 g           50 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttggat gtgaaatccc tcggcttaac cgaggaactg cgttcaatac tgccgtgctc
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgagagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009930
VERSION     KHUT01009930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           89 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggagatg
       61 taggcatgtt gtgaaatccc tcggctcaac cggggaattg cttcgtgaac cacatttctt
      121 gaggccggta agggcggtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccaat ggtgaagaca ggccgctggg ccggttctga cgctgatgca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009931
VERSION     KHUT01009931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctcg
       61 caagtcagat gtgaaatccc tcggcttaac caaggaagtg catttgaaac tgcaaggctt
      121 gagtactgga gagggtcgca gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcgacctggt cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009932
VERSION     KHUT01009932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           89 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggatcag
       61 caagtcagat gtgaaatctc gaggctcaac ctcgaaactg cgtctgaaac tgctagtcta
      121 gaatatcgga gggggaaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggg ggcgaaggcg ccttcctgga cgattattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009933
VERSION     KHUT01009933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          104 g           43 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggctcgg
       61 caagtcgtgt gtgtaagccc ctggctcaac tgggggaggt catgcgagac tggcgggctt
      121 gaggacagca gaggagcgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gcgctctggg ctgtgtctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009934
VERSION     KHUT01009934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           97 g           41 t
ORIGIN      
        1 gacagaggcc ccgagcgtta atcggaatta ctgggcttaa agggtgcgta ggcgggtccg
       61 caagtgcttt gtgaaatccc ccggctcaac cggggaacag cggggcatac tgcgggtctt
      121 gaggcaggta ggcgccgccg gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg ggcggctggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009935
VERSION     KHUT01009935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           91 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcgggagat
       61 taagtcatat gtgaaagccc tgggcttaac ctaggaatgg cgtatgatac tgattttctt
      121 gagtagggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctcatactga cgctgaggca cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009936
VERSION     KHUT01009936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 gacggggggt gcaagcgttg ttcggaatca ttgggcgtaa agcgtgtgca ggtggctctg
       61 caagtcagat gtgaaagccc cgggcttaac ccgggaagtg catttgaaac tgcagagctt
      121 gagtatggaa gagggtcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgacctggt ccaatactga cactcataca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009937
VERSION     KHUT01009937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           88 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggattta ctgggcgtaa agcgtacgca gacggaccgt
       61 caagtcggga gtgaaagccc tgggctcaac ccaggaacgg ctttcgatac tagcggtctt
      121 gagtttggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gcttcctggc ccaatactga cgttcaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009938
VERSION     KHUT01009938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatcca gcagcttaac tgttgaagag cctttgatac tggcgagctc
      121 gagggaggta ggggtttgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcgaactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009939
VERSION     KHUT01009939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           87 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggacggt
       61 taagtaggaa gtgaaaggtc ggagctcaac tccaacattg ctccctatac tggctgtctt
      121 gagtcccgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctttctgga cgggtactga cgctcaggcg cgaaagccga
      241 ggtagcgaac agg
//
LOCUS       KHUT01009940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009940
VERSION     KHUT01009940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacataggga gcgagcgtta tccggaatta ctgggtgtaa agggagcgta ggcgggaatg
       61 caagtcagat gtgaaaacca cgggcttaac ctgtggactg catttgaaac tgcgtttctt
      121 gagtgaagta gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gcttgctggg cttttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009941
VERSION     KHUT01009941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           59 c          109 g           37 t
ORIGIN      
        1 cacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggttact
       61 cgcatccctg gtgaaagccc cgggcttaac ccgggagggt ccagggagat ggggtgactg
      121 gaggcagggc gagggcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009942
VERSION     KHUT01009942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           84 g           54 t
ORIGIN      
        1 tacgtaccgt ccgaacgtta ttcggaatta ttgggcttac agggtgcgta ggcggcccag
       61 caagtcaggt gtgaaatccc acagcataac tgtggaactg cgcttgaaac tgctaggctt
      121 gagggagata gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacacctgt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01009943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009943
VERSION     KHUT01009943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           47 c           96 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggttggc
       61 taagtccgat gtgaaatctc tcggcttaac tgggaggagt catcggatac tggtcggctt
      121 gaggaaggta gaggagagta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctctctggg cctttcctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01009944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009944
VERSION     KHUT01009944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggtcctt
       61 taagtccttt gtgaaatccc ggggctcaac tccggatgtg cagaggatac tggaggacta
      121 gagaccggta gaggcgagtg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacacctgt ggcgaaggcg gctcgctggg ccggttctga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009945
VERSION     KHUT01009945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           82 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggagata
       61 gaagtcagat gtgaaatcct ggggctcaac ctcagaactg catttgaaac tctatccctt
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01009946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009946
VERSION     KHUT01009946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttag
       61 taagtcgatc gtgaaagccc ttggcttaac caaggaactg cggtcgaaac tactagacta
      121 gagtatcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009947
VERSION     KHUT01009947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           98 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcccga
       61 cgagtcgagg gtgaaagcct gtggcttaac cacagaatgg ccttggatac tgttgggctt
      121 gagaccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag agattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ctggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009948
VERSION     KHUT01009948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgaac
       61 caagtcagag gtgaaatccc agagcttaac tctggaactg cctttgaaac tggtttgctt
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagcggtg aaatgcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009949
VERSION     KHUT01009949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           87 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgggca ggcggatctt
       61 taagtaggaa gtgaaaggcg ggagcttaac tcctggactg ctccctatac tgggggtctt
      121 gagtgccgga gaggaccagg gaacgggttg tgtagcggtg aaatgcgttg atataactcg
      181 gaacaccaat ggcgaaagca ctggtctgga cggcaactga cgctcatcca cgaaagccaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01009950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009950
VERSION     KHUT01009950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           84 g           57 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagcct agggctcaac cctagaagtg catttgatac tgcgaagctt
      121 gagtgctaga gaggttacta gaattgttgg tgtagtggtg aaatacgtag atatcaacag
      181 gaataccggt ggcgaaggcg ggtaactggc tagacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009951
VERSION     KHUT01009951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgcg
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagagt cactcgatac tgcacgactt
      121 gaaggcagga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009952
VERSION     KHUT01009952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           96 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgta ggcggggctg
       61 tcagtctgct gtgaaagctt ccggctcaac tggaagaagc cagcggatac tacagttctg
      121 gaatccggca gaggtcggca gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaataccgaa ggcgaaggcc gccggctggg tcgagattga cgctgaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009953
VERSION     KHUT01009953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacagggggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttcga
       61 taagtcagat gtgaaagcct ggggcctaac tccagaagtg catttgaaac tgtcgagcta
      121 gagtgatgta gaggatggcg gaatacccag tgtagaggtg aaattcgtag agattgggta
      181 gaacaccggt ggcgaaggcg gccatctggg cattaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009954
VERSION     KHUT01009954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggatagt
       61 taagttggat gtgaaagccc tgggcttaac ctaggaattg catacaaaac tggcgatctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009955
VERSION     KHUT01009955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           86 g           60 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccctc
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tggggggctt
      121 gaatgaagtt gaagtgggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agtgaagaca gctcactaag cttctattga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009956
VERSION     KHUT01009956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggcttgg
       61 cgtgtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgccagacta
      121 gagtactgga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga cagtcactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009957
VERSION     KHUT01009957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           85 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcaatg
       61 taagttgaat gtcaaatctc ggggctcaac cctgtagccg cattcaatac tgcgatgcta
      121 gagtgcttaa aagggagccg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaagcg ggctcctagg aagcaactga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009958
VERSION     KHUT01009958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           80 g           53 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgca gacggctcat
       61 taagtcacct gttaaattct ccggcttaac cggaggccag cgggtgaaac taataagcta
      121 gagggtgaga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggc tcactcctga cgttcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009959
VERSION     KHUT01009959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagtttgat gtgaaatccc tgggcttaac ctaggaactg cgtcgaatac tggataacta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009960
VERSION     KHUT01009960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           57 c          107 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggttggc
       61 cacgtcgtgc gtgaaatctc tcggctcaac tgggaggggc cgtgcgatac gggtcgactg
      121 gaggcaggta gggggacgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01009961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009961
VERSION     KHUT01009961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           93 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggtttat
       61 caagttggga gtgaaagccc gaggcttaac ctcggaattg cttccaagac tggtaggcta
      121 gagagtggta gaggatggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gccatctggg ccatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009962
VERSION     KHUT01009962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           95 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccga
       61 taagtcggtt gtgaaagtcg agggctcaac cctcgaatgc cgaccgaaac tgtcgggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009963
VERSION     KHUT01009963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           95 g           40 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtgcat
       61 taagtctgat gtgaaagcgc ttggctcaac caagcgatgc cattggaaac tagtgcactg
      121 gagtctggca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ccagtactga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009964
VERSION     KHUT01009964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           87 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agggtgcgca ggcggatcga
       61 taagtcaggt gtgaaatctc ggggctcaac cccgaacgtg cgcctgaaac tattgatcta
      121 gaatactaga gagggaaggg gaatttctca tgtaggggta aaatccgtag agatgagaag
      181 gaacaccaga ggcgaaggcg ccttcctgga tagtgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009965
VERSION     KHUT01009965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           82 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtgcgta ggcgggaact
       61 cgcgtgagat gcgaaatctt caggctcaac ctaaagactg tatctcatac ggagtacctt
      121 gaggatggca gaggctagtg gaattactgg tgtagcagtg aaatgcgtag atatcagtag
      181 gaacaccaat ggcgaaggca gctagctggg ccacccctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac tgg
//
LOCUS       KHUT01009966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009966
VERSION     KHUT01009966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           95 g           47 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttgga
       61 caagttgagt gtgaaagctc ccggctaaac tgggagagga cattcaagac tgttcgacta
      121 gaggatggta gaggggcgta gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaataccagt ggtgaagacg acgccctggg ccattactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009967
VERSION     KHUT01009967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          102 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcga
       61 aaagtccggg gtgaaatccc tccgctcaac gggggaacgg cctcggatac tgtcggcctg
      121 gagggggata ggggcgagcg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg gctcgctgga tcctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01009968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009968
VERSION     KHUT01009968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           84 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtacgg
       61 aaagttggat gtgaaatccc acagcttaac tgtggaactg cattcaaaac ttccgaacta
      121 gagtatgaga ggggtaaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg ctttactggc tcattactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009969
VERSION     KHUT01009969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggctata
       61 taagtcagtg gtgaaagctc cccgcttaac ggggaaactg ccattgaaac tgcatagctt
      121 gaatatggtt gaagtggatg gaatatatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gttcactaag ccatcattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01009970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009970
VERSION     KHUT01009970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           91 g           47 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatga ttgggcataa agggtaggta ggtggtgaga
       61 taagtctgtg gtgaaagcct taagcttaac ttaagaagtg cctcagaaac tatctgacta
      121 gagtcctgga gagggtcgtg gaattctcgg tggagcggtg aaatgcgtag agatcgagag
      181 gaacatcaga ggcgaaggcg gcgacctgga aaggaactga cactgaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009971
VERSION     KHUT01009971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           97 g           45 t
ORIGIN      
        1 gacgaaggtg gcgagcgttg ttcggaatca ctgggcataa agcgcacgta ggcggcggcg
       61 taagttggat gtgaaatccc ccggcccaac cggggaactg cgtccagtac tgcgttgctt
      121 gaggatccaa ggggtgcatg gaattcccgg tggagcggtg aaatgcgttg atatcgggag
      181 gaactccggc ggcgaaagcg gtgcactggt ggatttctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009972
VERSION     KHUT01009972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcgcg
       61 caagtcggat gtgaaatctt ggggctcaac cccgaaactg cattcgatac tgcgtggctc
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009973
VERSION     KHUT01009973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           85 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgtgt
       61 taagtgtggt gttaaatcgt ggggctcaac cccgtcgatg catcacatac tggcacacta
      121 gaggacggca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag agattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccaatcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01009974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009974
VERSION     KHUT01009974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtgaa
       61 taagtcagcg gtgaaatctc cgggcttaac tcggaaactg ccattgatac tattcgtctt
      121 gaatatcctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacctat tgcgaaggca gctcactacg ggattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009975
VERSION     KHUT01009975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctgat
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgatcagctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01009976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009976
VERSION     KHUT01009976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          101 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 caagtcgatg gtgaaagccc gaggctcaac ttcgggtcgg ccactgatac tgctaggctt
      121 gagtcgacta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg gtcgaactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009977
VERSION     KHUT01009977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           81 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcaggg gtgaaagacg gtagctcaac tatcgcagtg cctttgatac tgatgggctt
      121 gaatctacta gaggcaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttgctatg gtagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009978
VERSION     KHUT01009978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           39 c           83 g           51 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggaataa ttgggcgtaa agggtgcgta ggcggtcctt
       61 taagtaagat gttaaaaacc tgggcttaat ctagggaatg cattttatac tgaaggactc
      121 gagtgtggga gaggaaacag gaattcctca tgtagaggtg aaattcgtag atatgaggag
      181 gaacaccaga ggcgaaggcg agtttctgga acacaactga cgctaaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009979
VERSION     KHUT01009979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           92 g           44 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggttcct
       61 taagtcagat gtgaaatcct gcggctcaac cgcggaactg catctgatac tggggaactt
      121 gaggacggta gaggtcggtg gaataccctg tgtaccggtg aaatgggcag atatagggtg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01009980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009980
VERSION     KHUT01009980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggtgcgta ggcggccttt
       61 taagtcaggt gtgaaagacc acagcttaac tgtggggttg cacttgaaac tgggaggctt
      121 gagtatcgga gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cgaaaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01009981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009981
VERSION     KHUT01009981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           99 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggctttt
       61 taagtcgggg gtgaaaatcc agggctcaac cctgggactg cctccgaaac tgggaagctt
      121 gagtactcta gagggtagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actgcctggg gggtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01009982             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009982
VERSION     KHUT01009982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           44 t
ORIGIN      
        1 tacggggggg ggcaagcgtt gttcggaatt actgggcgta aagggcgcgt aggcggcctg
       61 ctaagtcgga cgtgaaatcc ctcggctcaa ccggggaact gcgtccgata ctggcgaggc
      121 tcgaatccgg gagagggatg tggaattcca ggtgtagcgg tgaaatgcgt agatatctgg
      181 aggaacacca gtggcgaagg cggcatcctg gaccggtatt gacgctgagg cgcgaaagct
      241 agggtagcaa acggg
//
LOCUS       KHUT01009983             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009983
VERSION     KHUT01009983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcgctg
       61 caagctagat gtgaaagccc cgggcttaac ctgggaggtg catttaggac tgcagtgctt
      121 ttgagtgcgg cagagggagg tggaattcca cgtgtagcag tgaaatgcgt agagatgtgg
      181 aggaacaccg atggcgaagg cagcctcctg ggccgacact gacgctcatg cacgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01009984             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009984
VERSION     KHUT01009984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           89 a           36 c           74 g           56 t
ORIGIN      
        1 tacgaggggg gcaagcgtta ttctaaatga ctgggcgtaa agggcacata ggcggttttt
       61 ttagttggct atgataaaaa acaaggctta accatgtaaa gtcatccaaa actaaaaaac
      121 tcgagtaaat gagaggagaa tggaatttct ggaggagagg taaaattcgt agatatcagg
      181 aggaagatcg aaagcgaaag caattctctg gcactttact gacgctgatg tgcgaaagcg
      241 tggggagcaa aaggg
//
LOCUS       KHUT01009985             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009985
VERSION     KHUT01009985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          101 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccctt
       61 taagtccgtc ggtcaaatgc cggggctcaa ccccgttgca tgccgaggat actggagggc
      121 tggagacacg tagaggcaag cggaattccg ggtgtagcgg tggaatgcgt agagatccgg
      181 aagaacaccg gaggcgaagg cggcttgctg ggcgtgatct gacgctgagg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01009986             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009986
VERSION     KHUT01009986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           75 g           60 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtggaa
       61 taagttactg gtcaaagtcg agctcaactt gatggaaatt agtaatactg ttctactaga
      121 ggacaggaga ggttaacgga actctcatga gtaggggtga aatccattga tccatggatg
      181 accaccaaaa aaggcgaagg cagttaacta ttctgttcct gacattgagg catgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01009987             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009987
VERSION     KHUT01009987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          100 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggtccgg
       61 cacgtctgtt gctgaaagcc cccggctcaa ccggggaagg ggcgcggata cggccggact
      121 ggagggaggt agggggatcg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gcggtcctgg accttttcct gacgctgaga cgcgaaagct
      241 agggtagcga acggg
//
LOCUS       KHUT01009988             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009988
VERSION     KHUT01009988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c          105 g           38 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatgtg
       61 gcaagtcggg agtgaaatcc cggggctcaa ccccgtggac tgctcccgat actgctcgtc
      121 tagggaccgg tagaggccgg tggaattccc ggtgtagcgg tggaatgcgt agagatcggg
      181 aggaacacct gtggcgaagg cggccggctg ggccggatcc gacgctgagg cgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01009989             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009989
VERSION     KHUT01009989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           68 c          107 g           29 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatca ctgggcgtaa agcgcgcgta ggcggcgccc
       61 tgcgtcggcg gtgaaagctc ccggctcacc cgggagaggg ccgccgaaac gggggcgctc
      121 acgagggcgg taggggctgg cggaacaccc ggagtagcgg tgaaatgcgt tgagatcggg
      181 tagaacaccg gtggcgaagg cggccagctg ggccgaccct gacgctgagg cgcgaaagcc
      241 aggggagcga acgga
//
LOCUS       KHUT01009990             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009990
VERSION     KHUT01009990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           45 c           78 g           53 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggattta ttgggcgtaa aggatgcgta gatggcagaa
       61 aaagttactg gttgaagtta ggctcaacct gatggaagtc agtaatacta ttctactaga
      121 ggacaggaga ggttggcgga actcacatga gtaggggtga aatccgttga tccatggagg
      181 accaccaaaa aaggcgaagg cagctaacta tcctgctcct gacattgagg catgaaagcg
      241 tggggagcaa accgg
//
LOCUS       KHUT01009991             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009991
VERSION     KHUT01009991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtctgtt ggtgaaatcc tggggcttaa ccccagaagt gccacggaaa cggtaagact
      121 ttgagtactg gagaggctag cggaattccc ggtgtagcgg tgaaatgcgt agagatcggg
      181 aggaacacca gaggcgaagg cggctagctg gacagtaact gacactcaac tgcgaaagcg
      241 tggggagcaa acagg
//
LOCUS       KHUT01009992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009992
VERSION     KHUT01009992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggttgtcctg
       61 taagtctgct gtgaaatccc tgagcttaac tcaggaagtg cggcggatac tgcaagacta
      121 gagtatcgga ggggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gcgcacttga cgattactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009993
VERSION     KHUT01009993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           74 g           63 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcgggctat
       61 taagtcagtg gtgaaagctt gcagcttaac tgtaaaattg ccattgaaac tgatagcctc
      121 gaatatggtt gaggtggttg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gatcactaaa ccattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01009994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009994
VERSION     KHUT01009994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           95 g           41 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggacct
       61 caagtggtgc atgaaagctc tcggcttaac cgagaaacgg tgtgccagac tgaggaccta
      121 gagggtggga gagggacgtg gaattccggg tgtagtggta aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcgtcctggc ccacaactga cgctcataca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01009995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009995
VERSION     KHUT01009995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           62 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccgt
       61 caggtccggg gtgaaagccc acggctcaac cgtggaagtg ccttggatac cggcggcctc
      121 gagggaggca ggggcgtgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01009996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009996
VERSION     KHUT01009996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           90 g           43 t
ORIGIN      
        1 gacagaggtc tcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtccgc
       61 taagtcatgc gtgaaatccc ccggctcaac cggggaacag cgcctgatac tggcgggctc
      121 gaggtcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg actcactgga tcgaatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01009997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009997
VERSION     KHUT01009997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgcg
       61 taagattgat gtgaaatccc tgggcttaac ctaggaactg catcgatgac tgcgtagcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01009998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009998
VERSION     KHUT01009998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           99 g           42 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggtcagg
       61 caagtgttgt gtgaaagcgg cccgcttaac gggtcgaggc catggctgac tgcccgactg
      121 gaggtcgtga gaggtccgtg gaattgccgg tgtagtggtg atatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca acggactggc acgatgctga cactgaggtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01009999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01009999
VERSION     KHUT01009999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggctggt
       61 caagtccgat gtgaaagctc ccggctcaac tgggaaaatg catcggaaac tgaccggctt
      121 gaaggtggga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010000
VERSION     KHUT01010000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtttgat gtgaaatctc cgggctcaac ccggaaatgg cattgaatac tatctcgcta
      121 gagagtcgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cgacttctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010001             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010001
VERSION     KHUT01010001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           83 g           52 t
ORIGIN      
        1 tacgtggggt gcaagcgtta tccggaatta ttgggcgtaa agagatgcgt aggcggtata
       61 tcaagttttt ggtgaaagcc tctggctcaa ccagagaatt gccaagaaaa ctgataaact
      121 tagagggaat cagggggtgc tggaactcgt ggtgtagtag taaaatgcgt tgatatcacg
      181 aggaacacca aaggcgaagg caggcacctg ggattcacct gacgctaagg ctcgaaagcg
      241 tggggagcga aacgg
//
LOCUS       KHUT01010002             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010002
VERSION     KHUT01010002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           80 g           60 t
ORIGIN      
        1 tacagagagc acaatcgttg tccggattta ctgggcgtaa agcgtttcgt aggcgactta
       61 ctaagttact tttcaaagac cagagctcaa ctttgggaag ggaagtaata ctggtaggat
      121 tagaattatg tcggggctat tggaactgtc ggtgtagggg tgaaatccgt tgatatcgac
      181 aggaactccg agggcgaagg cagatagcta ggcatatatt gacgctgatg aacgacagct
      241 aggggagcga aaggg
//
LOCUS       KHUT01010003             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010003
VERSION     KHUT01010003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           39 c           82 g           55 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggttaaa
       61 taagttagcg gtgggaaatc agggctcaac cctgtggaag ttgctaatac tgtctaacta
      121 gaaggcagga gaggttaacg gaactccctg tggagcggta aaatgcgtta gatataggga
      181 agaacaccaa gaggcgaagg cagttaacta ttctgttctt gacattgagg cacgaaagcg
      241 tggggagcaa aaagg
//
LOCUS       KHUT01010004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010004
VERSION     KHUT01010004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtctga
       61 taagttcggc gtgaaatttt ccggcttaac cggaggatgt cgtcggagac tgtcggacta
      121 gagggtgata gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg tcacacctga cgctgatacg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010005
VERSION     KHUT01010005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           39 c           71 g           63 t
ORIGIN      
        1 gacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta ggctgtttgt
       61 taagttggat gttaaatctt tgagctcaac tcaaaatttg tatccaaaac tggcaagcta
      121 gagacaggta gaggtaaaca gaattctgtg tgtaggggta atatccgtag atatacagag
      181 gaataccaaa agcgaaggca gtttactgga ccttgtctga cgctaaagaa cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010006
VERSION     KHUT01010006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           86 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggcggacgag
       61 caagtcagat gtgaaatctc ggggctcaac cccgaaagtg cacctgaaac tattcgtcta
      121 gagtattgga gagggtaggg gaatttctca tgtaggggta aaatccgtag agatgagaag
      181 gaacaccaga ggcgaaggcg cctacctggc caattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010007
VERSION     KHUT01010007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           48 c           82 g           47 t
ORIGIN      
        1 tacgtagggt acgagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaaatg
       61 ccagtcagat gtgaaagccc ggggctcaac cccggaacag catttgaaac tacatttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01010008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010008
VERSION     KHUT01010008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgccta ggtggccaag
       61 caagtcggat gtgaaagccc ccggctcaac cggggaattg catccgatac tgcatggctt
      121 gagtcctgga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga caggtactga cactgacgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010009
VERSION     KHUT01010009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgcg
       61 taagtgagat gtgaaatccc cgggcttaac ctgggacgtg catcttatac tgcgtaactg
      121 gagtacaaga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ttgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010010
VERSION     KHUT01010010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           93 g           45 t
ORIGIN      
        1 gacgaaggtg gctagcgttg ctcggattta ctgggcataa agcgcgcgta ggtggtcgtg
       61 taagcggggg atgaaagccc atggctcacc catggaactg tcccccggac tgcacggctc
      121 gaggaaggaa ggggagaatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg attctctggt cctttcctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010011
VERSION     KHUT01010011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggttatc
       61 taagtctggt gtgaaatccc ggggcctaac ctcggaactg cactggatac tggatagcta
      121 gaggacgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gccatctggc tcgtacctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010012
VERSION     KHUT01010012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtctggt gtgaaagctc gcagctcaac tgcgaaatgg cactggaaac taccctgctg
      121 gaggcttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01010013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010013
VERSION     KHUT01010013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggtgcgg
       61 tgtgtttggt gtgaaatctc ccggctcaac cgggaggggc catcgaaaac tgccgtgctg
      121 gagtgcggaa gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacctgt gtcgaaggag gctgcctggg ccgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010014
VERSION     KHUT01010014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           86 g           58 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggcggatata
       61 taagttagat gtgaaaagcc agggcttaac cttggtcgtg catttaagac tgtatgtcta
      121 gagtttgtaa gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca gccccctggt atagaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010015
VERSION     KHUT01010015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           95 g           40 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggttccg
       61 cgagtcgggt gtgaaagccc ggggctcaac cccggaaggg cactcgatac tacggagctg
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010016
VERSION     KHUT01010016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggcggtttgt
       61 caggtccgat gtgaaagccc cgggcctaac ccgggaagtg cattggaaac cggcagactt
      121 gaatgcggga gaggagagcg gaattcctgg tgtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010017
VERSION     KHUT01010017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           82 g           57 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggtcttg
       61 taagtcagag gtgaaatccc ggagcttaac tccggaactg cctttgatac tacaaggctt
      121 gagtatggaa gaggttgatg gaatttcggg tgtagcagtg aaatgcgtag atatccgaaa
      181 gaacaccagt ggcgaaggcg gtcaactggt ccattactga cactaaagca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010018
VERSION     KHUT01010018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcaagg
       61 taagtttgat gtgaaatctt cgagctcaac tcggaaactg cattgaatac tatcttgctt
      121 gaggattgga ggggaggctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctaga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010019
VERSION     KHUT01010019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c           98 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgcc gtgaaatccc gaggcttaac ctcgggtctg cggtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010020
VERSION     KHUT01010020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           87 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaataa ctgggcgtaa agagtgcgta ggcggatatt
       61 taagttagaa gtgaaatacc tgggctcaac ctgggaaggt cttttaatac tgggtatcta
      121 gagttagtca gaggggggtg gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaacaccaat ggcgaaggca gccccctggg atgagactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010021
VERSION     KHUT01010021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c          101 g           44 t
ORIGIN      
        1 tacgtaggag acgagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggcgggtccg
       61 caagtctgct gtgaaagttc ctggcttaac cgggaaacgt cggtggaaac tgcgagcctg
      121 gaggacggta gaggtaggta gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaatacctgt ggcgaaggcg gcctactggg ccggacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010022
VERSION     KHUT01010022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttcgt
       61 taagtcagag gttaaagact ggggctcaac cccaggagtg cctttgatac tggcgggctt
      121 gaggacggca gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg actttctggg ccgttactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010023
VERSION     KHUT01010023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgacg
       61 aaagtcggac gtgaaatccc tgggctcaac ccaggaagtg catccgatac tgcgttgctt
      121 gaatcccgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010024
VERSION     KHUT01010024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttcga
       61 taagtcagat gtgaaatccc taggcttaac caaggaactg catttgaaac tgtcaaacta
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010025
VERSION     KHUT01010025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           92 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggctgca
       61 taagccggtc gtgaaatccc cgggcttaac ctgggaattg cgatcgggac tgtgcggcta
      121 gagtgttgga gaggacagcg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gctgtctggc caaacactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010026
VERSION     KHUT01010026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtctaat gttaaagacc agagctcaac tccggcatgg cattggaaac tggcaagcta
      121 gagtgtggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ccttgctgga ccattactga cgctgaggaa cgaaagccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01010027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010027
VERSION     KHUT01010027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          103 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 cgagtccggg gtgaaagccc ccagcttaac tggggaaggg ccttggatac tggtcgtctg
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010028
VERSION     KHUT01010028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcctgt
       61 caagttcgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tgataggctt
      121 gaggcaagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc cttagcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010029             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010029
VERSION     KHUT01010029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           48 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggaatgg
       61 tccgtccgat gtgaaagtgc taccgcttaa cggagcaaag ccatcggaaa ccgccattct
      121 tgagaccgga agaggtgagt ggaattgcca gtgtagcggt ggaatgtgta gatattggca
      181 agaacaccgg tggcgaaggc ggctcactgg tccggttctg acgctgagtg cgcgaaagcg
      241 tggggagcga acagg
//
LOCUS       KHUT01010030             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010030
VERSION     KHUT01010030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           85 g           51 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggccgag
       61 caagttagtg gtgggaaatc gaggctcaac ctcgtggaag ccagtaatac tgttcggctc
      121 gaggatagga gaggttaacg gaactccatg tggaggggta aaatccgcag atatatggat
      181 gaacaccaat aaggcgaagg cagttgacta ttctactcct gacattaagg cacgaaagcg
      241 tggggagcaa aacgg
//
LOCUS       KHUT01010031             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010031
VERSION     KHUT01010031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           84 a           32 c           72 g           67 t
ORIGIN      
        1 tacgagaggg gcaagcgtta tccggaataa ttgggcgtaa agggtccgta ggcggtattt
       61 tgtatgcttt tagtaaaaga caaaagctta acttttgaaa tgctttaagt acagatttac
      121 ttgagtttaa tagaggatag tagaattcca tgtggagggg tggaatccta agatatatgg
      181 aggaatgcca atagcgaaag caactatctg gattaatact gacgttgagg gacgaaagcg
      241 taggtagcaa acagg
//
LOCUS       KHUT01010032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010032
VERSION     KHUT01010032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           91 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgat
       61 taagttagat gtgaaattcc cgggcttaac ctggggactg catttgatac tgattgacta
      121 gagtatggta gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actctctggg ccaatactga cgctgaggcg cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01010033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010033
VERSION     KHUT01010033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          100 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctggg
       61 taagcgtgtg gtgaaagtcc ggggctcaac cccggatctg ccgtgcgaac tgcttagctt
      121 gagcaatgta gaggcgggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcccgctggg catttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010034
VERSION     KHUT01010034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtagga
       61 taagttagct gtgaaatccc cgggctcaac ctgggcaggt cagctaagac tgttttacta
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010035
VERSION     KHUT01010035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          102 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcaggt
       61 caagtctgtg gtgaaaggcg tcggctcaac cggcgcaggg ccatggatac tggcttgctc
      121 gaggggcgta ggggtcgtgg gaactgccag tggagcggtg aaatgcgttg atattggcag
      181 gaacgccggt ggcgaaagcg cacgactgga cgcttgctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010036
VERSION     KHUT01010036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcttc
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaactg ccattgaaac tgtgagactg
      121 gagtatgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcattactga cgctcaggcg cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010037
VERSION     KHUT01010037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c          100 g           47 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctgat gtgaaacctc caggctcaac ttggagcttg cattaggaac tgccgtgcta
      121 gagtgcggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcggt ggcgaaggcg gctctctgga ccgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010038
VERSION     KHUT01010038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 caggtcaggg gtgaaatccc gcagctcaac tgcggcagtg cctttgatac cgccgatctc
      121 gagggaggca ggggagcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgctctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010039
VERSION     KHUT01010039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          101 g           42 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggactta ctgggcgtaa agcgcgcgca ggcggacaag
       61 tatgtgccgt gtgaaagcgc cggacttcct ccggtgaggt catggtagac agcttgtctt
      121 gaggctcaga gaggtcggtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccagg ggcgaaagcg gccgactggc tgagtactga cgctgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010040
VERSION     KHUT01010040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           58 c           96 g           36 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggttcgt
       61 acagtctgat gtgaaatcac ccggctcaac cgggcgaggc cattggaaac tcacgaactg
      121 gagggcgata gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg tcgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010041
VERSION     KHUT01010041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggcggttcag
       61 tgtgtcggtt gtgaaatccc cgggcttaac ctgggaactg catccgaaac tgctgggcta
      121 gagtgtggta gagggcggta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgat ggcgaaggca gccgcctggg ccacaactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010042
VERSION     KHUT01010042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtgaaa
       61 taagttggat gtgaaagact cgagcttaac ttgagggttg cattcaatac tgttttacta
      121 gagtactgta gaggaaggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gccttctgga cagtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010043
VERSION     KHUT01010043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           81 g           55 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggtgtaa agggtgtgta ggcggtcttg
       61 ttagtcgtgc gtcaaatctc cgggcttaac ccggaacctg cgcgcgaaac ggcaagacta
      121 gaagatgcaa gaggtctgta gaactcatag tgtaggggtg aaatccgttg atattatggg
      181 gaataccaaa agcgaaggca gcagactggt gcactcttga cgttgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010044
VERSION     KHUT01010044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           82 g           59 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agggcatgta ggcggtttat
       61 taagttgaga gtgaaatccc gaagcttaac ttcggaactg ctttcaaaac tggtagacta
      121 gagataggta gaggatggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacatcaga ggtgaaaacg gccatctggg ccttatctga cgctaaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010045
VERSION     KHUT01010045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           85 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggatatt
       61 taagtcggat gtgaaagccc ggggctcaac cccggaacgg catttgaaac tatttatcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010046
VERSION     KHUT01010046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgta ggcggccctt
       61 caagtctaat gtgaaatccc tgggctcaac tcaggaagtg cattggatac tggtgggctt
      121 gagtcctgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccagt ggcgaaggcg accacctgga cggagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010047
VERSION     KHUT01010047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           74 g           62 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggtctta
       61 taagtcagtg gtgaaatctt ctcgcttaac gggaaaactg ccattgatac tgtaggactt
      121 gagtatagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacttag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctataactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010048
VERSION     KHUT01010048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           94 g           54 t
ORIGIN      
        1 tacgtagggt ccgagcgtta tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 tgcgtcggga gtgaaaaccc agggcttaac tctgggcttg cttccgatac gggcagactt
      121 gaggtattca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg aattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010049
VERSION     KHUT01010049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           93 g           43 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggtctgt
       61 taagcagggt gtgaaatccc ccgtctcaac cggggaactg cgccctgaac tggcggactg
      121 gagtgagata ggggtgcgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccgga ggcgaaagcg gcgcactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010050
VERSION     KHUT01010050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggttatg
       61 taagtcgagt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac tacatagcta
      121 gaggacagta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctggg ctggacctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010051
VERSION     KHUT01010051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcttg
       61 aaagtcgggt gtgaaatccc tcggcttaac caaggaagtg cattcgaaac ttcaagactt
      121 gagtactgga gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggt cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010052
VERSION     KHUT01010052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           88 g           54 t
ORIGIN      
        1 tacagaggtg gcaaacgttg tccggattta ttgggtgtaa agggcatgta ggtggttttg
       61 taagtcaaag gtgaaatggc ccggcttaac caggtcattg cctttgatac tgcaaggctt
      121 gagtacggaa gaggagagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctggt ccgaaactga cactgagatg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010053             255 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010053
VERSION     KHUT01010053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>255
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           50 t
ORIGIN      
        1 tacaggggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggtagtt
       61 gaagtctgct gttcaagacc agggcttaac cctgggccgg cagtggaaac tcagctgctt
      121 gagtgcggta ggggcagagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccgat ggcgaaggcg ctctgctggg cctttacact gacactgaga gacgaaagct
      241 aggggagcaa acggg
//
LOCUS       KHUT01010054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010054
VERSION     KHUT01010054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacggggggt gctaacgtta tccggaatta ttgggcgtaa agcgcatgta ggtggtttgg
       61 taagtcagca attaaagacc gacgcttaac gtcgggagtg ttactgatac tgccagactt
      121 gaggaaaaca ggggcaagcg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca gcttgctggg ttttacctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010055
VERSION     KHUT01010055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcgggacga
       61 taagtccggt gtgaaatccc tcggctcaac cggggaattg cactggatac tggcgacctt
      121 gagtcctgga gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctgga cagtgactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010056
VERSION     KHUT01010056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaacag cctttgatac tggtggtctc
      121 gagtgaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcgtgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010057
VERSION     KHUT01010057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcggatcgt
       61 taagttagag gtgaaagccc agggctcaac cctggaattg cctttgatac tggcgatctt
      121 gagtatggga gaggtaagcg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactggc ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010058
VERSION     KHUT01010058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           84 g           56 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggcttt
       61 taagtcagat gtgaaatacc agagctcaac tctggtgctg catttgaaac tggaagtctt
      121 gagggatggc ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc tatttcctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01010059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010059
VERSION     KHUT01010059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggttttg
       61 taagttgggg gtgaaatccc cgggctcaac ccgggaatgg ccttcaagac tgcaaagcta
      121 gaggacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010060
VERSION     KHUT01010060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 aacgtaggag acgaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtctgc
       61 taagtccggc gtgaaatctt tcggcttaac cgggggatgt cgtcggagac tggcggacta
      121 gagggtgata gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg tcatacctga cgctaatacg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010061
VERSION     KHUT01010061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgttcga
       61 caagtcggat gtgaaagccc tgggctcaac tcaggaagtg cattcgaaac tgtcgagctg
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010062
VERSION     KHUT01010062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggcgagg
       61 taagtcgaat gtgaaagccc gtggcttaac cacggaagtg cattcgaaac tgccttgctc
      121 gagtgtcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg accgcctgga cgaccactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010063
VERSION     KHUT01010063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           78 g           60 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattta ttgggtgtaa agggtgtgta gacggtttat
       61 taagttcgtt gttaaaactt ttggcctaac caaaagctcg cggtgaaaac tgatagacta
      121 gaggatgaaa gagaggagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaagca gctccttggt tcattcctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010064
VERSION     KHUT01010064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatt
       61 cacgtccggg gtgaaagcct ccagctcaac tggagaagtg cctgggatac gggatgcctc
      121 gaggaaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acacactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010065
VERSION     KHUT01010065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagtcggat gtgaaaacac gaggctcaac ctcgtgcgtg catccgaaac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01010066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010066
VERSION     KHUT01010066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgt
       61 taagttatct gtaaaatccc tgggctcaac ctgggcatgc cagataaaac tgatggactc
      121 gagtacggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010067
VERSION     KHUT01010067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacgaggggg gcaagtgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggcgct
       61 taagtgggat gtttaaaacc ggggcccaac cccggagcgg cattccaaac tgggcgtctt
      121 gaatagggga gaggtcgatg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacaccgat tgcgaaggca gtcgactgga cccatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010068
VERSION     KHUT01010068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           84 g           49 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgcgca ggcggcacac
       61 taagtcaact gtcaaatctc ccggcctaac cgggaatcgg cggtagatac tggtgagcta
      121 gagggtggaa gagagaagtg gaattcttgg agtagaggta aaattcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010069
VERSION     KHUT01010069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           96 g           41 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggcggccctg
       61 taagtccgct gtgaaagtca gtggctcaac cactgaatgc cggtggatac tgcagggcta
      121 gagtccggaa gaggcgggtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gcccgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010070
VERSION     KHUT01010070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggtgatctt
      121 gagtgaggta ggggcagctg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaactcccgt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010071
VERSION     KHUT01010071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaataa ctgggcataa agcgtacgta ggtggatttg
       61 caagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgaaac tgcaaatctg
      121 gaatgtcaga ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga tgaccattga cactgaggta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010072
VERSION     KHUT01010072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 aacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggtggttttt
       61 taagtttcgt gtgaaatccc atggcttaac catggaactg cacggaaaac tggaaagctt
      121 gagtacggga gaagagggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctctctaga ccgatactga cgctcatacg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010073
VERSION     KHUT01010073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtgggg
       61 tgagtttgag gtgaaatctc cgggcccaac ccggaaaatg ccttgaatac tatcccgctg
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ggtctctgga cgattcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010074
VERSION     KHUT01010074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagtgcgta ggttgtaatg
       61 taagtcaggt gtgaaatccc gtagctcaac tacggaactg cgcttgatac tgtattacta
      121 gagtcttgga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagg ggcgaaggcg actgcctggc caaagactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010075
VERSION     KHUT01010075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           62 c          116 g           33 t
ORIGIN      
        1 tacgggggat gcgagcgttg tccggattca ctgggcgtaa agggcgcgta ggcggcccgg
       61 tcagtcggcg ctgtaagtgc ggggctcaac cccgtgatgg cgccgggacg gccgggctgg
      121 ggcgcggcag agggagacgg aatgccgggt gtagtggtga aatgcgtaga gatccggcgg
      181 aacaccggtg gcgaaggcgg tctcctgggc cgagggccga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010076
VERSION     KHUT01010076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggcggcttgt
       61 taagtcctgt gtgaaagccc taggcttaac ctaggaagtg cgcaggaaac tggcgagctt
      121 gagtgctgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cagacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010077
VERSION     KHUT01010077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 gacagggggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggacccg
       61 tgagtgcttc gtgaaaaccc acggctcaac cgtggaattg cggtgcatac tgcgggtctt
      121 gagggaagta tgggcggaca gaactatcgg tggagcggtg aaatgcgtag atatcgatag
      181 gaatgccgat ggcgaaggcg gtccgctggg cttctcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010078
VERSION     KHUT01010078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgaga
       61 taagttaggt gtgaaatccc cgggcttaac ctgggaactg catttaagac tgttgagcta
      121 gagtagaaga gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttactgga ttcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010079
VERSION     KHUT01010079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttgtg
       61 caagacagat gtgaaatccc tgggcttaac ctaggaattg catttgtgac tgcatagcta
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010080
VERSION     KHUT01010080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttggg
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg cattcgatac tacttggcta
      121 gagtactgga gaggatggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggc ggcgaaggcg gccatctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010081
VERSION     KHUT01010081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggcttag
       61 caagtcagat gtgaaatccc ggggcctaac tccggaagtg catttgaaac tgctaggcta
      121 gagtgctgta gaggatggcg gaatacccag tggagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gccatctggg cagcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010082
VERSION     KHUT01010082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           85 g           51 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ctgggcgtaa agcgtgtgta ggtggttgtg
       61 ttagtctctc gttaaattct tcggcttaac cggggacctg cgggggaaac ggcacgacta
      121 gagggtgcaa ggggtcgacg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtcgactggt gcactcctga cactgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010083
VERSION     KHUT01010083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggatatc
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaactg cattcgatac tgggtgtcta
      121 gagtatggta gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010084
VERSION     KHUT01010084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccatt
       61 taagtagggt gtgaaagccc ccggctcaac cggggaattg cgtcctaaac tggatggctc
      121 gagtgggata gaggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010085
VERSION     KHUT01010085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           91 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtttgg
       61 taagtcttct gtgaaatcta cgggcttagc ctgtaacgtg cagaggaaac tgtcaggctt
      121 gagtgtcgga gaggaaaggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cctttctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010086
VERSION     KHUT01010086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           91 g           51 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtcagga gtgaaatctt tcggcttaac cggaaaactg cttttgatac tgacagcctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010087
VERSION     KHUT01010087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgacc
       61 tttgttagag gtgaaatccc gaggctcaac ttcggaattg cctttgaaac ggggttgcta
      121 gagtccgaga gaggatggcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggc tcggtactga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010088
VERSION     KHUT01010088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggatatt
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catctgaaac tggatgacta
      121 gagtatggta gaggacagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacaccagt ggcgaaggcg actgtctggg ccaatactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010089
VERSION     KHUT01010089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           43 c           77 g           54 t
ORIGIN      
        1 tacgaaggga gcaagcgtta ttcggaatca ctgggcgtaa agggcgtgta ggcggttaat
       61 taagtcagtt gttaaattcc ccagcctaac tggggccaag caattgaaac taattggcta
      121 gagtacagaa gagagaagtg gaattatcgg agtagcggta aaatgcgtag atctcgatag
      181 gaacaccaat ggcgaaggca gcttcttggt ctgttactga cgctaaaatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010090
VERSION     KHUT01010090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           82 g           59 t
ORIGIN      
        1 tacgagagcc tcgagcgtta tccggaatta ttgggcgtaa agggtgcgta ggttgttctg
       61 ttagtctttt gttaaatccc ggggcttaac ttcggaatcg caaaagaaac ggcaggactt
      121 gaaagtgtga gaggcgtacg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca gtacgctggc gcatatttga cactgaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010091
VERSION     KHUT01010091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggtaaag
       61 caagtaagat gtgaaagccc tgggcttaac ctgggaagtg catcttaaac tgcttaacta
      121 gagtacggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgccagt ggtgaagacg actacctgga tcgatactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010092
VERSION     KHUT01010092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacgaagggg actagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatatg
       61 ccagtcaggg gtgaaatccc ggggctcaac ctcggaacag cctttgatac agcatatctt
      121 gagtccgata gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactgga tcggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010093
VERSION     KHUT01010093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggcaggta ggcggttttg
       61 taagtccggc gtgaaatccc aaggctcaac cttggaactg cgttggaaac tacatgactt
      121 gagtatcgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cggttactga cgctgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01010094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010094
VERSION     KHUT01010094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           97 g           49 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcttgg
       61 taagtcagtt gtgaaatccc tcggctcaac cgaggaattg cgtctgatac tgtcgagctt
      121 gagtcccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga cgggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010095
VERSION     KHUT01010095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctg
       61 caagtccggg gtgaaagccc ccagcttaac tggggaagtg ccttggaaac tgcaggtctc
      121 gagggaggta ggggcatgtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010096
VERSION     KHUT01010096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacgaggggg gcaagtgttg ttcggaatta ctgggcgtaa agggagcgta ggcggatgtt
       61 taagtcgggt gtttaaaacc ggggcccaac cccggagcgg cacccgaaac tggatatctt
      121 gaataggtca gaggctagtg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacacctat tgcgaaggca gctagctggg accatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010097
VERSION     KHUT01010097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctagg gtgaaatcct ggggcttaac cccagaagtg ccctggaaac ggtgagactg
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caggtactga cactaaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010098
VERSION     KHUT01010098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggtggttagg
       61 taagtccgta atgaaatctc ccggcttaac cgggaggggt ttacggatac tgccagactt
      121 gagtgtggga gagggtgctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaagca ggcacctggc ccaatactga ctctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010099
VERSION     KHUT01010099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggttgtc
       61 caagtcgtgt atgaaagcgc tccgcttaac ggggcgaggc tagacgagac tgggcgacta
      121 gaatcagaca gaggcaagcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgcaggcg gcttgctggg tctgtattga cgctcagaca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010100
VERSION     KHUT01010100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtgaag
       61 tgtgtctgac gtgaaatccc tcggcttaac cggggaactg catcggaaac tactttgctg
      121 gagtgcgaga gagggtggcg gaatttccag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccgga ggcgaaggcg gccacctgga tcgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010101
VERSION     KHUT01010101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttata
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaattg catcttagac tatatggctg
      121 gaggtcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgaatctga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010102
VERSION     KHUT01010102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           84 g           52 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcgggttag
       61 taagtcagga gtgaaatccc ggggcctaac cccggaactg ctcttgagac tgctaatcta
      121 gaattcggta gaggttaatg gaatatccag tgtagaggtg aaattcgcag atattggatg
      181 gaacaccagt ggcgaaggcg attaactggg ccgacattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010103
VERSION     KHUT01010103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           84 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgtgta ggtggatatt
       61 taagtcgggt gtgaaatccc tgggctcaac ctgggaattg cattcgatac tgattgtcta
      121 gagtatggta gaggaaggcg gaactttcgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gccttctgga ccaatactga cacttaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010104
VERSION     KHUT01010104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgcga
       61 caagttagat gtgaaatgcc tgggcttaac ctaggaactg catttaagac tgtcgtgcta
      121 gagaccggga gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt tgcgaaggcg gccacctgga ccggcactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010105
VERSION     KHUT01010105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           94 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcccgt
       61 tatgtcggct gtgaaagccc atggctcaac catggaagtg cagccgaaac tggcgggcta
      121 gagtcctgga gagggtggcg gaatgcccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccacctgga cagcgactga cgctaagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010106
VERSION     KHUT01010106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtttgat gtgaaagctc cgagcttaac tcggaaattg catcgaatac tctctcacta
      121 gagggttgta ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010107
VERSION     KHUT01010107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaacag ccttggatac tggtcgtctg
      121 gagggaggta ggggcggacg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gtccgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010108
VERSION     KHUT01010108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           97 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccac
       61 ccagtccggg gtgaaatctt tcggctcaac cggaaaaggg ccttggatac tggtggtctg
      121 gaggaaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccttttctga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010109
VERSION     KHUT01010109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggggttg
       61 caagtggtgt atgaaatctc caggcttaac ctggaggggt tatgccagac tgcaattcta
      121 gaggacgaga gaggggggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gccccctggc tcgtacctga cgctcagcca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010110
VERSION     KHUT01010110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          105 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtggag
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgctcgactc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010111
VERSION     KHUT01010111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctatc
       61 cgcgtcggat gtgaaagctc aaggctcaac cttgagatgc cattcgatac gggatagcta
      121 gaggtagcta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg gcttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01010112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010112
VERSION     KHUT01010112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           54 c           93 g           39 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggaacgc
       61 caagtcagat gtgaaagacc ggggctcaac cccggaacgg catccgatac tggcgatcta
      121 gagggctcga ggggagtgtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccagt ggcgaaggcg acactctgga gagcaactga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010113
VERSION     KHUT01010113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           52 c           99 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggccgag
       61 caagtcatgc gtgaaatctc ccggctcaac tgggaggggt cgtgtgatac tgcttggctt
      121 gagcaaggta gggggcagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctgga cctttgctga cgctaatgcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01010114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010114
VERSION     KHUT01010114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           94 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggttgct
       61 taagtcagat gtgaaagccc tgggctcaac ctgggaatgg catttgaaac tgggcagcta
      121 gagtgtgata gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga tcaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010115
VERSION     KHUT01010115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtccca
       61 aaagtctact gtgaaatccc cgggcttaac ccgggaagtg cggtagatac tctgggacta
      121 gagtactgga ggggttcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga cagtaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010116
VERSION     KHUT01010116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           91 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggctaaa
       61 taagtcgagt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac tgtttagcta
      121 gaggacggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctggg ccgttcctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010117
VERSION     KHUT01010117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggat gtgaaaccct gaggctcaac ctcaggcctg cattcgatac gggcagacta
      121 gaattcggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatattga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010118
VERSION     KHUT01010118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           91 g           44 t
ORIGIN      
        1 tacagaggcc acaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggctcga
       61 caagtgcttt gtgaaatccc ggggctcaac cccggaacag cagggcatac tttcgagctt
      121 gaggcaggta ggggtcgcga gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggtgaagacg cgcgactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010119
VERSION     KHUT01010119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           93 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctcag
       61 caagtcgggg gtgaaatctt tcagctcaac tggaaaatag cctccgatac tgctggactc
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tcttctctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010120
VERSION     KHUT01010120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 aaagtctgct gtgaaatccc tgggcttaac ccaggaagtg cggcagatac tctaagactt
      121 gagtgccgga ggggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gcgcactgga cggcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010121
VERSION     KHUT01010121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           83 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agagtgcgta tgcggaaata
       61 taagttagat gtgaaaagcc tgggcttaac ctaggtcgtg catttaagac tgtatatcta
      121 gagttagtga gaggggcgaa gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca tcgccctggc atgagactga cgctgaggca cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01010122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010122
VERSION     KHUT01010122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           99 g           41 t
ORIGIN      
        1 tacggagagt gcaaacgttg ttcggaatca ttgggcgtaa agggcgcgca ggcggcatgt
       61 caggtcgggt gtgaaagccc tgggctcaac ccaggaagtg cgctcgaaac cgatgtgcta
      121 gagtaccgga gggagaggtg gaattcctgg tgtaggggtg aaatccgtag agatcaggag
      181 gaacaccggt ggcgaaggcg acctcttggc cggctactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010123
VERSION     KHUT01010123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           94 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtttgg
       61 taagtcagat gtgaaagccc agggctcaac cttggaagtg catctgaaac taccgaacta
      121 gagtatcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accgcctgga cgaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010124
VERSION     KHUT01010124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           83 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctcca
       61 taagtcagcg ttgaaatacg gcagcttaac tgtcggactg gcattgatac tgtggagctt
      121 gagtaaagtt gaggtaggcg gaattgatgg tgtagcggtg aaatgcttag atatcatcaa
      181 gaacatcgat tgcgaaggca gcttactaag ttttaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01010125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010125
VERSION     KHUT01010125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c          100 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggtcggc
       61 tcagtcgggg gtgaaaatcc agggctcaac tctgggactg cctccgatac tggttgactt
      121 gagtactcta gagggtagtg gaattgccgg tgtagcagtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg actgcctggg gggaaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010126
VERSION     KHUT01010126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggtttat
       61 tgggtgtgat gtgaaagcct cgggcttaac ctgagaagtg catcgcaaac taatagactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010127
VERSION     KHUT01010127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           83 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca gacggtttgt
       61 taagtcaact gttaaatctc ctggcctaac cgggagcccg cagtagaaac tggcgaacta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgttcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010128
VERSION     KHUT01010128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           92 g           40 t
ORIGIN      
        1 gacggaggag gcaagcgtta tccggaatga ttgggcgtaa agggtctgta ggtggccatt
       61 caagtctgtc gttaaagccc gaggctcaac ctcggaacag cggtggaaac tggaaggcta
      121 gagagcagta ggggtcacgg gaatttccag agtagcggtg aaatgcgtag agactggaaa
      181 gaacaccaac agcgaaggca cgtgactggg ctgactctga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01010129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010129
VERSION     KHUT01010129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           83 g           55 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcagattta ctgggcgtaa agtgcgcgta ggtggatatt
       61 taagtcagtt gtgaaacccc tgggcttaac ctaggaactg catctgatac tggatgtcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010130
VERSION     KHUT01010130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgcgg
       61 caagtcggga gtgaaatctc tgggcttaac ccagagacgg cttctgaaac tgccgtgctc
      121 gagtgcggga gaggcgagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctcgctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010131
VERSION     KHUT01010131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccaac
       61 taagtcggac gtgaaatccc aaggctcaac cttggaactg cgtccgatac tggatggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010132
VERSION     KHUT01010132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtcgtg
       61 tatgtctccc gtgaaagcag acggctcaac cgtcttccgt cgggagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccccg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagcactga cactgaggcg cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010133
VERSION     KHUT01010133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           84 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaatcct gcagcttaac tgtaggcgtg ccattgatac tggaaggctt
      121 gagtacagac gaggttggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gctaactagg ctgtaactga cgctgaggca cgaaagtgcg
      241 gggatcgaac agg
//
LOCUS       KHUT01010134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010134
VERSION     KHUT01010134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c           98 g           38 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 aaagtcggat gtgaaatccc ggggctcaac cccgggcctg cacccgatac tcccgggctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01010135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010135
VERSION     KHUT01010135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agcgaacgta ggcggttact
       61 taagtctgga cttaaagact gaggctcaac ctcaggagtg gttcggatac tgggtgacta
      121 gaggatgtca gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg gctttctggg acattcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01010136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010136
VERSION     KHUT01010136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgaaac tgcaaagctt
      121 gagtgctaga gaggttatta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg gataactggc tagacactga cactgagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010137
VERSION     KHUT01010137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtcttg
       61 aaagtctgct gtgaaatccc cgggcttaac ctgggaagtg cggcagatac tccaagacta
      121 gagtactgga ggggttcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgaacttga cagttactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010138
VERSION     KHUT01010138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           54 c           77 g           52 t
ORIGIN      
        1 tcccagcagt ccaagttgca accacatttg ttgggtctaa aacatccgta gcgcgctttg
       61 taagtcgcct gtgaaatcct gcctcttaag ggcagaagtg cgagcgatac tgcagagcta
      121 gggaccggga gacgtaagaa gtatgcatgg agtagcggta aaatgtgttg atcctatgca
      181 gactaacaat agcgaaggca tcttacgagc acggatccga ccgtgaggga tgaaggctag
      241 gggcgcaaag tgg
//
LOCUS       KHUT01010139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010139
VERSION     KHUT01010139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           90 g           54 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggctggg
       61 taagtttgat gtgaaatcct ggggctcaac cctggaactg cattgaatac tgctcggcta
      121 gagtactgga gaggaaactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg agtttctgga cagttactga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010140
VERSION     KHUT01010140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           82 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttta
       61 taagtcagtg gttaaagact ctcgctcaac gagagggatg ccattgatac tgtagaactt
      121 gagtactgta gaggtaggcg gaattggtag tgtagcggtg aaatgcatag atactaccag
      181 gaacaccgat tgcgaaggca gcttactgga gagtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010141             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010141
VERSION     KHUT01010141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           56 c          109 g           43 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggattca ctgggcgtaa agggcgcgta ggcggctgac
       61 tcagtctggt ctataaggtg tgggcttacc ccgcacacgg attggggacg ggttggctgg
      121 gggtcggtcg agggaagcgg aatgccgggt gtagtggtga aatgcgtaga gatccggcgg
      181 aacaccggtg gtgaaggcgg cttccttggc cgaacccgac gctgaggcgc gagagcgcgg
      241 ggagcgaacc gg
//
LOCUS       KHUT01010142             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010142
VERSION     KHUT01010142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtccat
       61 taagtctggt gtttaagccc ggggctcaac cccggtacgc acgggaaact ggtggacttg
      121 agtgcaggag agggaagcgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcgg ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010143             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010143
VERSION     KHUT01010143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agggtgcgca ggcggcaatt
       61 taagtccgtg gtaaaagact gaagctcaac ttcaggtggc catggaaact gggttgctag
      121 agtgcaggag agggtagtgg aattccatgt gtagcggtaa aatgcgtaga tatatggagg
      181 aacaccagtg gcgaaagcgg ctacctggcc tgtaactgac gctcatgcac gaaagcgtgg
      241 ggagcaaata gg
//
LOCUS       KHUT01010144             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010144
VERSION     KHUT01010144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           41 c           87 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaataa ctgggcgtaa agggcgcgta ggcggtgaga
       61 taagctggtt gtgaaagaat tgggcttaac ctaataaagc aaacaggact gtcaaactag
      121 agggaaggag aggtgagaag aatttccgga gtagcggtga aatgcgtaga taccggaaag
      181 aataccagaa gcgaaggcga ctcactggac ttaacctgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010145             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010145
VERSION     KHUT01010145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcat
       61 taagtctggt gtttaaggct agggctcaac cctggttcgc actggaaact gttgaacttg
      121 agtgcagaag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010146             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010146
VERSION     KHUT01010146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           27 c           68 g           72 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcggattaa ctgggcgtaa agggcgcgta gatggtttgt
       61 tatgtgtgct attaaattat aaagcttaac tttatactgt agtatataca attaaactag
      121 agttaggaag agggtagtag aatttctaat gtaaaggtga aatttgataa aattagagag
      181 aataccaatg gcgtaggcaa ctacctggtc cttaactgac attgaggtgc gaaggtatgg
      241 ggagcaaata gg
//
LOCUS       KHUT01010147             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010147
VERSION     KHUT01010147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatca ttgggcgtaa agggcgtgta ggcggttcta
       61 taagtcaggt gttaaatctc cgggctcaac ccggatccgc acttgatact gtagaactag
      121 agaatggtag aggtaagcgg aactgcaagt gtagcggtga aatgcgtaga tatttgcagg
      181 aacaccagtg gcgaaggcgg cttactgggc catttctgac gttgagacgc gaaagctagg
      241 ggagcaaacg gg
//
LOCUS       KHUT01010148             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010148
VERSION     KHUT01010148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           92 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ttgggcgtaa agggtgcgta ggcggtttct
       61 tatgcgcggg gtgaaaggca atggctcaac cattgttagc ctcgcgaact gggaggcttg
      121 agtgcaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctttctggac tgtaactgac gctgaggcac gaaagtgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010149             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010149
VERSION     KHUT01010149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtgatt
       61 taagtttggg gtataaggcc ggggctcaac cctggatcgc cctgaaaact gggtcacttg
      121 agtgcaggag agggaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010150             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010150
VERSION     KHUT01010150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           40 c           75 g           55 t
ORIGIN      
        1 tacggggggg gcaagcgtta ttcaatttaa ctgggcgtaa agcgtctgca ggcggcaagt
       61 ttcgttattc gggaaaaatt aaagcttaac tttaaaagac ggataaaacg tactttgcta
      121 gagtcataga gaggatcgta gaatttctga agtagggatg aaatccaatg acttcagaag
      181 gaataccaaa ggcgaaggca acgatctggc tatgactgac gctcagagac gaaggcgtgg
      241 ggagcaaatg gg
//
LOCUS       KHUT01010151             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010151
VERSION     KHUT01010151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           53 t
ORIGIN      
        1 gacggaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtccgta ggcttttcac
       61 caagtctgac attaaagtgc agagcttaac tctgtgatgt gttggaaact agtggaatag
      121 agtcatacag aggcaactgg aatgccgtgt gtaggggtaa aatccgttga tccacggtag
      181 aacgccaaaa gcgaaggcag gttgctgggt atgtactgac gctgagggac gaaagcgtgg
      241 ggagcgaagg gg
//
LOCUS       KHUT01010152             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010152
VERSION     KHUT01010152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           38 c           77 g           58 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggattaa ttgggcgtaa aggatgagta ggctgtttag
       61 taagtcatag gtaaaatctt caggcataac ctgaaatcgc tggtgatact gctaaactag
      121 agtgggatac aggaaagtgg aattcctagt tgagaggtga aattcgtaga cattaggagg
      181 aacgccaaag gcgaaagcag ctttctggta cctatctgac gctgaatcat gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010153             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010153
VERSION     KHUT01010153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           76 g           60 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gacggtaaaa
       61 caagttactg gttgacgtcg agctcaactt gactgaaatt agtaatacta ttttgctaga
      121 gaatggaaga agttgatgga agtctacgag gaggggtgaa atccattgat ccgtagggta
      181 ccaccaaaag gcgaaggcaa ttgactattc catttctgac gttgaggcat gaaagcgtgg
      241 ggagcaaacc gg
//
LOCUS       KHUT01010154             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010154
VERSION     KHUT01010154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcgt
       61 taagtctgga gtttaagttc ggggctcaac cccgtatcgc tctggaaact ggggaactgg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010155
VERSION     KHUT01010155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           46 t
ORIGIN      
        1 tacgtaggaa actagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggcatgg
       61 caagtctgtt gtgaaatcgc ccggctcaac tgggcggggt caatggaaac tgccaggctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gctcgctggg ccggtcctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010156
VERSION     KHUT01010156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           99 g           48 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcgaaa gtgaaattcc gaggcttaac ttcgggactg ctttcgatac gggcagacta
      121 gaggtaggca ggggagagtg gaaccccagg tgtagcggtg gaatgcgcag atatctggga
      181 gaacaccggt ggcgaaggcg gctctctggg ccttacctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010157
VERSION     KHUT01010157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           97 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttgga
       61 atcgtcggat gtgaaaggcc tgggcttaac ctgggaacgg cattcgatac ggtctgacta
      121 gagtgtggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010158
VERSION     KHUT01010158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c          100 g           49 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcgacc gtgaaaacct ggggcttaac ccttggcttg cggttgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010159
VERSION     KHUT01010159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgagta ggcggcctgc
       61 caagtctcat gtgaaatctt ctggctcaac tggaaggggt catgggaaac tggtaggctc
      121 gagggtaaca gaggaaagcg gaattcccgg agtagcggtg aaatgcgtag agaccgggag
      181 gaacaccaga ggcgaaagcg gctttctggg ttacacctga cgctgaggcg cgaaagcatg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010160
VERSION     KHUT01010160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcggtt gtgaaaactc taggcttaac ctggagcttg caaccgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010161
VERSION     KHUT01010161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           66 c          105 g           31 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactta ctgggcgtac agcgcgcgca ggcggccagg
       61 tatgtgcccg gtcacatcgt cccgctcaac gggacgcggc ccgggtagac agcatggcta
      121 gaggccgaga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc tcggccctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010162
VERSION     KHUT01010162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtcaat
       61 taagtcagat gtgaaatccc tgggcttaac ttaggaattg catatgatac tgattggcta
      121 gagtgcggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc ccgttactaa cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010163             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010163
VERSION     KHUT01010163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           91 g           45 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacagt
       61 taagtcagcg gtcaaaacgg gcggctcaac cgcctgccgc cgttgaaact ggcagtctag
      121 agtgggcgag aagtacgcgg aatgcgcggt gtagcggtga aatgcataga tattgcgcag
      181 aactccgatt gcgaaggcag cgtaccggcg cccgactgac gctgaggcac gaaagcgtgg
      241 ggatcgaaca gg
//
LOCUS       KHUT01010164             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010164
VERSION     KHUT01010164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcttt
       61 taagttcggt gtttaaaccc aaggctcaac cttgggtcgc atcggaaact gggagactgg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010165             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010165
VERSION     KHUT01010165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           84 g           49 t
ORIGIN      
        1 ccccagcgcc ccaagtggca atcgtgtatt tggcctaaag cgtccgtagc cggtttgcta
       61 aatttcctgt gaaatggtcc gcctcaatcg gtccacgcgc agggaacact ggcagacttg
      121 agaccgggag gggttagagg tatgtctggg ggagcggtaa aatgttataa tcccagatgg
      181 accgtcgatg gcgaaggcat ctaaccagaa cggatctgac ggtgagggac gaaagctggg
      241 ggagcgatcc gg
//
LOCUS       KHUT01010166             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010166
VERSION     KHUT01010166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggctgtt
       61 taagtctggt gtataaacct ggggctcaac tccgggtcgc actggaaact ggatagcttg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctgggc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010167             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010167
VERSION     KHUT01010167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           92 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttttt
       61 taagtctgat gtataagttc aaggctcaac cttgggatgc attggaaact gggggactgg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010168             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010168
VERSION     KHUT01010168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggtggcttgt
       61 taagtcaggt gtcaaaatgc ggggctcaac cccgtatcgc acttgaaact ggcaggcttg
      121 agtgcaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctttctggac tgcaactgac actgaggcgc gaaagctagg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010169             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010169
VERSION     KHUT01010169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcat
       61 taagtctggt gtttaaggct gcggctcaac cgcagttcgc actggaaact ggtgaacttg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010170             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010170
VERSION     KHUT01010170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           88 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaga
       61 taagctagat gtgaaataac tgggcttaac ctggtagagc atataggact gtataactag
      121 agaggggtag aggtgagcgg aatttccgga gtagcggtga aatgcgtaga taccggaaag
      181 aacaccagag gcgaaggcgg ctcactggac tctaactgac gctgaggcgc gaaagcatgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010171             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010171
VERSION     KHUT01010171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcgt
       61 taagtctggt gtataagccc ggggctcaac cccggttggc actggaaact ggcggactgg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010172             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010172
VERSION     KHUT01010172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttcac
       61 atagtcgggt ggttaagctc ggggctcaac cccgagtcac actggaaact ggtggacttg
      121 agtgcagaag aggagagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctctctggtc tgcaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010173             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010173
VERSION     KHUT01010173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtggtg
       61 taagtctgat gtttaaacct gaggctcaac ctcggatcgc attggaaact gtgccactgg
      121 agtgtaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010174             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010174
VERSION     KHUT01010174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           63 c          112 g           35 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggattca ctgggcgtaa agggcgcgta ggcggccgat
       61 tcagtccggt cttcaagcgc ggggctcacc cccgcgaagg gccggggacg ggtcggctgg
      121 gggccggtcg agggagacgg aatgccgggt gtagtggtga aatgcgtaga gatccggcgg
      181 aacaccggtg gtgaagacgg tctcctgggc cggtaccgac gctgaggcgc gagagcgcgg
      241 ggagcgaacc gg
//
LOCUS       KHUT01010175             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010175
VERSION     KHUT01010175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           88 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggaatta ctgggcgtaa agggtacgca ggcggccaaa
       61 taagtcaggt gtgaaaggta tcggctcaac cggtgcaagc acttgaaact gtatggcttg
      121 agtacaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcga ctttctggac tgatactgac gctgaggtac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010176             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010176
VERSION     KHUT01010176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttagt
       61 taagtctcat gtttaaaccc agggctcaac cttgggtcgc atgggaaact ggttaacttg
      121 agtgcaggag aggaaagtgg aattccacgt gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010177             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010177
VERSION     KHUT01010177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           82 g           53 t
ORIGIN      
        1 tacgtaggtt gcaagcgtta tccggaatta ctgggcgtaa agcgtctgta ggcggcgagg
       61 taagtagggg ataaaagctc tgggcccaac ccagaatgtg ttccctaaac tgctttgcta
      121 gagaaatgga gaggtgaatg gaattcctgg tgtaggggta aaatccgttg atatcaggag
      181 gaacgctaaa agcgaaggca gttcactggc catttctgac gctgagagac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010178             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010178
VERSION     KHUT01010178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcaggg
       61 aaagtcttgt gtcaaagacc ggagctcaac tccgggttgc atgggaaact gcccagcttg
      121 agtgtaggag agggaagtgg aattccacgt gtagcggtga aatgcgtaga gatgtggagg
      181 aacaccagtg gcgaaggcga ctttctggcc tataactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010179             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010179
VERSION     KHUT01010179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           43 t
ORIGIN      
        1 tacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggtcgtg
       61 caagtgtcgt gtgaaagcag ctcgctcaac gagctgaggc atggcagact gtatggctag
      121 agcccgtgag aggtcggtgg aattgccggt gtagtagtga aatacgtaga gatcggcagg
      181 aacactcatg gggaagccag ccgactggca cggtgctgac actgaggtgc gacagcgtgg
      241 ggagcaaacg gg
//
LOCUS       KHUT01010180             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010180
VERSION     KHUT01010180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           92 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agagcatgta ggcggttttt
       61 taagtccgga gtgaaaatgc ggggctcaac cccgtatggc tctggatact ggaagacttg
      121 agtgcaggag aggaaagggg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtg gcgaaggcgc ctttctggac tgtgtctgac gctgagatgc gaaagccagg
      241 gtagcgaacg gg
//
LOCUS       KHUT01010181             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010181
VERSION     KHUT01010181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggagcgta ggcggggaga
       61 caagttgaat gtctaaacta tcggcttaac tgatagtcgc gttcaaaact atcactcttg
      121 agtgcagtag aggtaggcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcgg cctactgggc tgtaactgac gctgaggctc gaaagcgtgg
      241 gtagcaaaca gg
//
LOCUS       KHUT01010182             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010182
VERSION     KHUT01010182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ttgggcgtaa agcgcgcgca ggcggttcat
       61 taagttaggt gttcaaggca acggctcaac cgttgtacgc acctaaaact ggtgggcttg
      121 agtgcagaag aggagagtgg aattcctggt gtagcggtgg aatgcgtaga tatcaggagg
      181 aacaccagtg gcgaaggcga ctctctggtc tgtaactgac gctgaggcgc gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010183             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010183
VERSION     KHUT01010183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatga ttgggcgtaa agggagcgca ggcgggaaag
       61 taagtctatc ttaaaagtgc ggggctcaac cccgtgaggg gatggaaact atttttcttg
      121 agtgcaggag aggaaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcgg ctttctggac tgtaactgac gctgaggctc gaaagccagg
      241 ggagcgaacg gg
//
LOCUS       KHUT01010184             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010184
VERSION     KHUT01010184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           37 c           83 g           58 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggttcgca ggtggtcctt
       61 taagtcagat gtgaaagact atggctcaac catggtaagc atttgaaact gaaggacttg
      121 agttaaggag aggaaagtgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aataccagtg gcgaaggcga ctttctggac ttatactgac actgaggaac gaaagcgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010185             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010185
VERSION     KHUT01010185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           52 c          109 g           45 t
ORIGIN      
        1 tacggggggt gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggcttta
       61 tcagtcggac ttttaagagc ggggctcaac cccgtggtgg gttcgggacg gtagagctgg
      121 ggcgcggtcg agggggatgg aatgccgggt gtagtggtga aatgcgtaga tatccggcgg
      181 aacaccggtg gtgaaggcga ttccctggac cgaggccgac gctgaggcgc gagagcgtgg
      241 ggagcgaacc gg
//
LOCUS       KHUT01010186             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010186
VERSION     KHUT01010186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           84 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agggtgagta ggcggttaat
       61 taagtctgaa gttaaaggca gtggctcaac cattgtatgc tttggaaact gaataactag
      121 agtatgatag aggtcggtgg aattccacat gtagcggtga aatgcgtaga tatgtggagg
      181 aacaccagtg gcgaaggcgg ccgactggat caaaactgac gctgaggcac gaaagcgtgg
      241 ggagcaaata gg
//
LOCUS       KHUT01010187             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010187
VERSION     KHUT01010187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           88 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ttgggcgtaa agagtgcgta ggtggtaacc
       61 taagcagggg gtgaaaggca aaggctcaac cattgtcagc cccctgaact gggctacttg
      121 agtgcaggag aggaaagcgg aattcctagt gtagcggtga aatgcgtaga tattaggagg
      181 aacaccagtg gcgaaggcgg ctttctggac tgttactgac actgaggcac gaaagtgtgg
      241 ggagcaaaca gg
//
LOCUS       KHUT01010188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010188
VERSION     KHUT01010188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           94 g           53 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtcagat gtgaaagccc cgggcttaac tcgggaattg catctgaaac tgctttgctt
      121 gagtcctaga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacctgc ggcgaaggcg gctacctgga tagtgactga cgctgatgcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010189
VERSION     KHUT01010189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           98 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctgtc
       61 cacgtcggat gtgaaagctc agggctcaac cctgagatgc cattcgatac ggggcagcta
      121 gaggcagcta ggggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg gctgttctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01010190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010190
VERSION     KHUT01010190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcgggaaat
       61 taagcgtggc ttgaaagccc ccggctcaac cggggaggga ggctgcgaac tggttttctt
      121 gagggcgata gaggagtgcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010191
VERSION     KHUT01010191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           99 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ttgggcttaa agggcgcgta ggcgggctgg
       61 ccagtccggg gtgaaagccc acggctcaac cgtggaagtg ccttggatac tgccagtctt
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010192
VERSION     KHUT01010192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattca ttgggcgtaa agggagcgta ggcggtcggg
       61 taagtcggat gtgaaatcct ggggctcaac ctcagaactg cattcgatac tgcttggcta
      121 gagtattgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg gatctctgga caactactga cgctgaggct cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010193
VERSION     KHUT01010193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           88 g           45 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggccgcacgg
       61 ttagtcagag gtgaaatccc ggagctcaac tccggaacgg cctccgatac tgccgtgctc
      121 gaggactgga gaggtatccg gaattaatgg tggagcagtg aaatgcgtag atatcattag
      181 gaacactagc ggcgaaggcg ggatactgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010194
VERSION     KHUT01010194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           82 g           57 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 caagtcagga gtgaaagccc acggcttaac cgtggaagtg cttttgaaac tgcttagctt
      121 gaatgtggaa gaggttagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaatacctgt ggggaagccg gctaactggt ccaacattga cactcagacc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01010195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010195
VERSION     KHUT01010195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcgtgta ggctgttatc
       61 taagtctggt gtgaaatccc ggggcttaac tccggaagtg cattggatac tggataacta
      121 gaggacgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccatctggc tcgttcctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010196
VERSION     KHUT01010196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgggca ggcggacctt
       61 taagtaggaa gtgaaaggcg ggagcttaac tcctggactg ctccctatac tgggggtctg
      121 gagtaccgga gaggacaagg gaacgggttg tgtagcggtg aaatgcgttg atataactcg
      181 gaacaccaat ggcgaaagca cttgtctgga cggtcactga cgctcatcca cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01010197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010197
VERSION     KHUT01010197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtccggg gtgaaagctt tcggcttaac cggaaaagtg ccttggatac tggcaggctg
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010198
VERSION     KHUT01010198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttga
       61 caagtcttat gtgaaatctt cgggctcaac ccgaagtctg catgggaaac tgtcgagctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010199
VERSION     KHUT01010199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacttc
       61 atagtcgggg gtgaaatcgt tcggctcaac cggaccaggg cccccgatac tgggagtctc
      121 gagggaggta ggggtcggtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gccgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010200
VERSION     KHUT01010200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           81 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtat agggcgcgta ggcggatctt
       61 caagtcggat gtgaaatccc agggctcaac cttggaactg catccgaaac tgaaagtcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaagcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01010201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010201
VERSION     KHUT01010201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           96 g           49 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggttctg
       61 catgtcgggt gtgaaatccc acggatcatc cgtggaacgg cattcgaaac tgcagggctg
      121 gagtgcggga gaggaaggtg gaattctcgg tgtagcggtg aaatgcgttg atatcgagag
      181 gaacgccggt ggtgaagacg accttctgga acgcaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010202
VERSION     KHUT01010202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           94 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctcga
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg caaacgaaac tatcgggctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcgacctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010203
VERSION     KHUT01010203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           84 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agcgcacgta ggtggaaaat
       61 taagttggat gtaaaatccc tgggcttaac ctaggactgt catccgatac tgattttcta
      121 gagtacagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010204
VERSION     KHUT01010204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcgtg
       61 caagtggaat gtgaaatccc tcggcttacc cgaggacctg cattccaaac tgcgcgactt
      121 gagtacggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccgatactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010205
VERSION     KHUT01010205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           93 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcagg
       61 taagtggaac gtgaaatccc tcggctcacc cgaggacctg cgtcccaaac tgcttggctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010206
VERSION     KHUT01010206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           82 g           57 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggattta ctgggtgtaa agggtgcgca ggcgggctgg
       61 taagtcagag gtgaaatctc ggcgctcaac gccgaaactg cctttgatac tattagtctt
      121 gaatacggta gagattcgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcgaattggg ccgttattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01010207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010207
VERSION     KHUT01010207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           58 c          108 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctttg
       61 taagggtgcg gtgaaagccc ggggcttacc cccgggtcgg ccgtgccgac tgcagggctt
      121 gggcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010208
VERSION     KHUT01010208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcgggg gtgaaatctt tcggcccaac cggaaaatag ctttcgatac tggcaggctc
      121 gagggaggta ggggcacgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga tctccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010209
VERSION     KHUT01010209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcacgta ggcggtggcg
       61 taagtcgggt gtgaaatccc tcggcctaac cgaggaactg cgcccgatac tgcgttgctt
      121 gaggaccaaa ggggtgcatg gaatttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgtcggc ggcgaaagcg gtgcactggt tggtctctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010210
VERSION     KHUT01010210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           82 g           55 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggtgtaa agggtgcgta ggcggtggta
       61 ttagtctttt gtcaaagctc cgagctcaac ttggaaaagg cagaagaaac ggtaccgcta
      121 gagagtgtga gaggtttgcg gaactaatgg tgtaggggtg aaatccgttg atatcattgg
      181 gaacaccaaa tgcgaaggca gcaaactggc acatatctga cgctcaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010211
VERSION     KHUT01010211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           62 c           84 g           44 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggtttta
       61 caagtcctcc gttaaatcca cctgctcaac agttgggctg cggaggatac tacagcgcta
      121 ggaggcggga gaggcaagcg gtactcagtg ggtaggggta aaatccactg atccattgaa
      181 gaccaccagt ggcgaaggcg gcttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010212
VERSION     KHUT01010212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggtttag
       61 caagtccgta atgaaatctc ccggcttaac tgggaggggt ttagggatac tgctaggctc
      121 gagtgtggta gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacctat ggcgaaagca ggttactgga ccaatactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010213
VERSION     KHUT01010213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           92 g           43 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatta ctgggcgtaa agggcgtgca ggcggccttg
       61 taagaggaat gtgaaacccc ctggctcaac cagggaactg cattccagac tgcaaggctt
      121 gagtacagga gaggatgggg gaattcccgg tgtagcggtg gaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggc ctgatactga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010214
VERSION     KHUT01010214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           86 g           50 t
ORIGIN      
        1 tacggagggt gctagcgttg ctcggaatta ctgggcgtaa agcgtacgca ggcggcttgg
       61 taagtcggat gtgaaatccc caggctcaac ctgggaactg catccgaaac tacctcgctt
      121 gagttcggga gagggaagtg gaattcctgg tgtagaggtg aaattcgttg atatcaggag
      181 gaacaccggc ggcgaaagcg gcttcctggc ccgatactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010215
VERSION     KHUT01010215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           45 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggcccgt
       61 taagtctgcg gtgaaatccc ggagctcaac tccggatatg ccgcggatac tggcgggcta
      121 gagaccagta gaggcgagtg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ctggttctga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010216
VERSION     KHUT01010216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           82 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtgagat gttaaaacgt gtggctcaac catatcgttg tatctcatac tggcgaacta
      121 gaggatggca gaggcaagtg gaatttctcg tgtagcagtg aaatgcgtag atatgagaag
      181 gaacaccaat ggcgaaggca gcttgctggg ccaaccctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010217
VERSION     KHUT01010217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggaagcg
       61 caagtccggt gtgaaagccc ccggctcaac cggggaaggt cgctggaaac tgcgcttctt
      121 gaggacgaca gagggaagtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctggg tcgtgcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010218
VERSION     KHUT01010218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agggttcgta ggcgggacga
       61 caagtcaaat gtgaaatccc tgggctcaac ccaggacgtg cctttgaaac tgtcgttctt
      121 gagtgccaga gagggtggtg gaattgctgg tgtaggagtg aaatccgtag agatcagcag
      181 gaacatcgga ggcgaaggcg accacctggc tgagcactga cgctgaggaa cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010219
VERSION     KHUT01010219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           92 g           50 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccac
       61 aaagtccggg gtgaaatctt tcggcttaac cggaaaattg ccttggatac tggtggtctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010220
VERSION     KHUT01010220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           73 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaatag
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaattg ccattgatac tgctaatctt
      121 gagtataatt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcactaaa ctataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010221
VERSION     KHUT01010221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcgggcgtc
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ttgggcatac tggacgcctt
      121 gaggtgctca ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gctcgctggg agcatgctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010222
VERSION     KHUT01010222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           43 c           91 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggctgt
       61 taagtctggt ttgaaagctg gtggcttaac catcagatgt ggctggatac tgggagtctt
      121 gaaattgttg gaggcagccg gaacgggtcg tgtagcggtg aaatgcatag agatgaccca
      181 gaacaccaat tgcgtaggca ggctgctacg actgatttga cgctgaggca cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010223
VERSION     KHUT01010223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 gacgtaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcacgta ggcggccaag
       61 tcagtcaggt gtgaaatacc tcggctcaac cgaggaactg cgcctgatac tgcttggctt
      121 gaggtccgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cggaatctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010224
VERSION     KHUT01010224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctta
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg ccccagaaac ggtaagactg
      121 gagtctcgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctgga cgagtactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010225
VERSION     KHUT01010225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           52 c          101 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggttcgg
       61 aacgtccgtt gtgaaatcgc ccggctcaac tgggcggggt cagcggatac gatcgaactg
      121 gagcaagcta gggggcaatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctggg gctttgctga cgctgatgcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01010226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010226
VERSION     KHUT01010226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggattca ctgggcataa agcgcacgta ggcggctagg
       61 tctgttcgat gtgaaagccc ccggctcaac cggggaattg catcggatac tgcctggctc
      121 gaggacggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgttactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010227
VERSION     KHUT01010227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          100 g           40 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtccggg gtgaaagctt tcggctcaac cggaaaaggg ccctggatac tggcgggctg
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg agatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010228
VERSION     KHUT01010228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           89 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcatgta ggcggacgtg
       61 caagtcaggc gtgaaatccc cgggctcaac ccgggaatcg cacttgaaac tgctcgtctt
      121 gagtaccgga gaggatagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cggtcactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010229
VERSION     KHUT01010229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggccaat
       61 caagtcagag gtgaaatgcc ggggcttaac cccggaattg cctttgaaac tgcttggcta
      121 gagtatggga ggggatagcg gaattccaaa tgtagaggtg aaattcgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga ccattactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010230
VERSION     KHUT01010230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           86 g           54 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgta ggcggcatgt
       61 taagtcaatc gttaaattcc tcggcctaac cggggacctg cggtagaaac tgatgtgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010231
VERSION     KHUT01010231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          106 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggctcgt
       61 caagtccggc ctgaaagccc ccggctcaac cggggagggt ggctggaaac tggcgggctt
      121 gagggcgaga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccctctggc tcgaccctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010232
VERSION     KHUT01010232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           87 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcgg
       61 aaagtcggac gtgaaatccc tgggctcaac ctaggaactg cgctcgaaac ttccgacctt
      121 gagtgatgga gaggatagtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gctatctgga catcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010233
VERSION     KHUT01010233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggatatt
       61 taagttggat gtgaaatccc tgggcttaac ctaggaattg catttgaaac tggatatctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010234
VERSION     KHUT01010234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggtctg
       61 tatgtcgatt gtgaaatccc cgggcttaac ctgggaattg cagtcgaaac tgcagatctt
      121 gaatgtttga gaggatggcg gaattcctgg agtagtagtg aaatacgtag atatcaggaa
      181 gaacacccga ggcgaaggcg gctatctggt gatacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010235
VERSION     KHUT01010235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcgggctat
       61 caagtcgcat gtgaaagccc acggctcaac cgtggaagtg catgcgaaac tggcagtctt
      121 gagtaccaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tggttactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010236
VERSION     KHUT01010236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtatgc
       61 caagtcgggc atgaaatcgc tcggctcaac cgggcgaggt tgtccgatac tggcagactg
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaagcg gccacctgga ctgtacctga cgctgaaacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010237
VERSION     KHUT01010237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           85 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtttgat gtgaaatctc ggagcttaac tccgaaactg cattgaatac tattcggctt
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactaaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010238
VERSION     KHUT01010238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcgggatgt
       61 caagctggat gtgaaagacc tgggcttaac ctgggggtgt cattcataac tgacgttcta
      121 gagtagggga gaggagagtg gaattcacag tgtagcggtg aaatgcgtag atattgtgag
      181 gaacatcgat ggcgaaggca gctctctggc ctactactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010239
VERSION     KHUT01010239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgagg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catttgatac tgctttgctt
      121 gaggactgga gaggagatcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaaggccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010240
VERSION     KHUT01010240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           89 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgg
       61 taagttgggt gtgaaagtcc tgggcttaac ctggggattg cgctcaatac tgccaagcta
      121 gagtactgta gaggaaggtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010241
VERSION     KHUT01010241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           95 g           41 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggacggt
       61 caagtcagtg gtgaaatcct ccggcctaac cggagaagag ctgttgatac tggccgtctc
      121 gagtggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cccctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010242             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010242
VERSION     KHUT01010242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           86 a           35 c           72 g           59 t
ORIGIN      
        1 tacgaagggg gcaggcgtta ttcagtataa ctaggcgtaa agggtccgta ggcggttcat
       61 tccgttgtta acaaaaaact aaggcttaac ctttgtaaga gtaacaaaac gtttgaacta
      121 gagtatttta gaggatagtg gaatttctaa gggagggatg aaatctaaag atcttagaag
      181 gaacaccata ggcgtaagcg actatctgga aaatactgac gctgagggac gaaagttagg
      241 ggagcaaatg gg
//
LOCUS       KHUT01010243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010243
VERSION     KHUT01010243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          100 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggtggcttct
       61 taagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggcgac tgggaggctt
      121 gaggcgagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaataccagt ggcgcaagcg gcaccctggc cttggcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010244
VERSION     KHUT01010244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtaggtg tcaagcgtta tccggaatta ctgggcgtaa agggcgcgca ggcggcctgt
       61 caagtttgac gtgaaatctc tcggcttaac tgggagggga cgttggagac tggcaagctt
      121 gaggtagtga gagggaggcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaagcg gccttctggc actaatctga cgctgaggtg cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010245
VERSION     KHUT01010245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c          101 g           39 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 gaagtcctgg gtgaaagccc ccggctcaac cggggaagtg cctgggaaac caccgagctg
      121 gagtgctgga gaggaaagca gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaatacctga ggcgaaggcg gctttctgga cagacactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01010246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010246
VERSION     KHUT01010246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 gacgaaggtg acaaccgttg ctcggaatca ctgggcataa agcgcgcgta ggtggctggg
       61 taagtccatg gtgaaatccc tcggctcaac cgaggaatgg ccgtggatac tgcctggctt
      121 gaggtcggga ggggtgagtg gaactctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacgccggt ggcgaaagcg actcactgga ccgcacctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010247
VERSION     KHUT01010247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 taagtcaaag gtgaaatccc tcggctcaac cggggaactg cctctgaaac tgcctcgcta
      121 gagtccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacacccgt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010248
VERSION     KHUT01010248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           54 c           86 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccctg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaatac tacagggctt
      121 gaggaagaca gaggtgagtg gaacttgcgg tggagcggtg aaatgcgttg atatcgcaag
      181 gaacaccagt ggcgaaagcg actcactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaag agg
//
LOCUS       KHUT01010249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010249
VERSION     KHUT01010249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           94 g           39 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agcgcatgca ggcggcaggc
       61 aaagtcggac gtgaaagctc ctggctcaac taggagaggc cgttcgatac tagccggcta
      121 gagggtgata gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctggg tcacacctga cgctaagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01010250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010250
VERSION     KHUT01010250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg tcaagcgtta tccggaatta ctgggcgtaa agggcacgca ggcggtctga
       61 gaagttcagc gtgaaagctc ccggcttacc tgggagaggt cgttgaaaac tctcagactt
      121 gagacagaga gaggaaggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgcaagcg gccttctggc ttttgtctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010251
VERSION     KHUT01010251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           94 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggattta ttgggcgtaa agggcgcgta ggcggctttt
       61 taagtcagat gtgaaagccc ggggcttaac cccggaagtg catttgaaac tgagaggctt
      121 gagtatggga gagggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctgga ccaatactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010252
VERSION     KHUT01010252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaagta ggtggttcca
       61 atagtctact gtgaaatccc tgggcttaac ccaggaactg cggtggatac tctggaactt
      121 gagtactgga ggggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaagcg gcgcactgga cagtaactga cactcaattg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010253
VERSION     KHUT01010253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggttggt
       61 caagttcgac gtgaaagctc ccggctcaac tgggagaggt cgttgaagac tggtcaactt
      121 gaggttatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc atgaacctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010254
VERSION     KHUT01010254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c          100 g           38 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcacgca ggcggaaggg
       61 gaagttctgg gtgaaagccc ccggctcaac cggggaagtg cctggaaaac cacctttctg
      121 gagtgctgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gcttcctgga cagacactga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010255
VERSION     KHUT01010255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggctcgt
       61 caagttcgac gtgaaagctc ccggcttaac tgggagaggt cgttgaaaac tgacgggctt
      121 gaggcaagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc cttgtgctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010256
VERSION     KHUT01010256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagaggtt ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaagctc cgggctcaac ccggaaatgt catcggatac tattcggctc
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacccgt ggcgaaggca agtccctgga aaactcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010257
VERSION     KHUT01010257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c           98 g           41 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcacgca ggcggctcgt
       61 tacgtgtgag gtgaaagctg cgggctcaac ccgcagtcgc ccttgcatac gggcgggctc
      121 gagccattca gaggtccctc gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaagacccac ggggaagcca ggggactggg aatgtgctga cgctcaggtg cgatagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010258             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010258
VERSION     KHUT01010258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcgggaatc
       61 taagtctgtc ttaaaagtgc ggggctcaac cccgtgatgg gatagaaact gggtttcttg
      121 agtgcaggag aggacagtgg aattcccagt gtagcggtga aatgcgtaga tattgggagg
      181 aacaccagtg gcgaaggcga ctgtctggac tgtgtctgac gctgaggcgc gaaagccagg
      241 gaagtgaacg gg
//
LOCUS       KHUT01010259             243 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010259
VERSION     KHUT01010259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 243)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..243
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>243
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           78 g           44 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtatgta cgtggctttt
       61 caactccgcc gtcaaatccc agggctcaac ccaggacaga tggtggaaac taccaagctg
      121 gagtacgtta ggggcatagg gaatttccgg tggagtggtg aaatgcattg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cgaaagctag gggagcaaat
      241 ggg
//
LOCUS       KHUT01010260             247 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010260
VERSION     KHUT01010260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>247
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcctgca ggcgatccgc
       61 caagtttgag gcgacaatca ctggcttaac tggtgaacgg cctcaaaaac tggaggactt
      121 gagagcgaga gagggacatg gaactccggg tggagcagtg aaatgcgtag agatccggaa
      181 gaacaccgaa ggcagtgtcc tggctcgttt ctgacgctga ggggcgaaag ccaggggagc
      241 gaacggg
//
LOCUS       KHUT01010261             251 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010261
VERSION     KHUT01010261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>251
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           50 t
ORIGIN      
        1 gacgaggggg gcaagtgttc tttggaatga ctgggcgtaa agggcacgta ggcggttaat
       61 cgggttgaaa gtgaaagtcg ccaaaaagtg gcggaatgct ctcgaaacca attcacttga
      121 gtgagacaga ggagtgtgga atttcgtgtg taggggtgaa atccgtagat ctatgaagga
      181 acgccaaaag cgaaggcagc tctctgggtc cctaccaacg ctggggtgcg aatgcatggg
      241 gagcgaacat g
//
LOCUS       KHUT01010262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010262
VERSION     KHUT01010262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           88 g           49 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggaatca ctgggcttaa agcgtacgca ggcggaccgg
       61 taagtacttt gtgaaatccc tcggctcaac cgaggaactg cttggtatac tgccggtctg
      121 gagcatggta gaggcggtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccgaa ggtgaagaca ggccgctggg ccattgctga cgctcatgta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010263             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010263
VERSION     KHUT01010263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           28 c           67 g           70 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agggtacgta ggtggtttgt
       61 tatgtgtact ataaaattat aaagcataac tttatactgt agtatagaca actaaactag
      121 agttaggtag agggtagtag aatttctaat gtaaaggtga aatttgataa atttagagag
      181 aataccaatg gcgtaggcaa ctacctgggc cttaactgac actgaggtgc gaaggtatgg
      241 gtagcaaata gg
//
LOCUS       KHUT01010264             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010264
VERSION     KHUT01010264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ttgggcgtaa agggagtgta ggcggatggg
       61 aaagtcaggc gtcaaatgga agcgctcaac gctttcaatc gtctgatact tcttgtctag
      121 aggggtggag aggagatcgg aattcacggt gtagcagtga aatgcgtaga tatcgtgagg
      181 aagaccaaag gcgaaagcag atctctggaa accttctgac gctgaggctc gaaggccaag
      241 ggagcaaacg gg
//
LOCUS       KHUT01010265             252 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010265
VERSION     KHUT01010265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>252
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c          100 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggcttgg
       61 tcagtctggt gtgaaaggtc ggggctcaac cccggttagc actggatact gctaggcttg
      121 agtgctggag agggtcgcgg aattcccagt gtagcagtga aatgcgtaga gattgggagg
      181 aacaccagtg gcgaaggcgg cgacctggac agtaactgac gctgaggcgc gaaagctggg
      241 ggagcaaacg gg
//
LOCUS       KHUT01010266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010266
VERSION     KHUT01010266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgggg
       61 taagtttgat gtgaaagccc cgggcccaac ccgggaatgg cattgaatac tattccgcta
      121 gaggatcgga agggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010267
VERSION     KHUT01010267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggctgtc
       61 caagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tagatagctc
      121 gaggcgagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg acaccctggc ctttgcctga cgctcaggtg cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010268
VERSION     KHUT01010268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           86 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttttg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgatac tgtagaacta
      121 gagtgtgata gaggatagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaggca attatctggg tcaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010269
VERSION     KHUT01010269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c          102 g           39 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgtgcgta ggcggacggg
       61 taagtgcgtg gtgaaagcct ccggctaaac tgggggagga ccaagcagac tgccggtcta
      121 gagtcggata gaggggtgcg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgccctggg tccgaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010270
VERSION     KHUT01010270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggcccca
       61 taagtctcgg gtgaaatccc tcggctcaac cgaggaaccg ccttggatac tgtggggctc
      121 gaggctggaa gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010271
VERSION     KHUT01010271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           46 c           80 g           51 t
ORIGIN      
        1 tacagagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggacctg
       61 caagtcggat gtgaaatccc ggggctcaac cccggaacag catttgaaac tacagatcta
      121 gagggtagaa ggagaaaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg attttctatt ttattcctga cgctgaggcg cgaaagcaag
      241 aggatcaaac agg
//
LOCUS       KHUT01010272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010272
VERSION     KHUT01010272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggtttgt
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cattggaaac tggcagacta
      121 gagtcggaca gagggccgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatcgat ggcgaaggca gcggcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010273
VERSION     KHUT01010273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggaccag
       61 taagtcggtt gtgaaagtcc agggctcaac cctggaaagc cggccgatac tgctggtcta
      121 gaatccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010274
VERSION     KHUT01010274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcggctttg
       61 tatgtcggat gtgaaatccc cgggctcaac ccgggaactg cacccgaaac tgcaaagctt
      121 gaatgcttga gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcggt ggcgaaggcg gccaactggc aatgcattga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010275
VERSION     KHUT01010275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtcccg
       61 taagtcggat gtgaaatccc tcggcttaac cgaggaagtg catccgaaac tgtgggactt
      121 gagtatggaa gagggtcgca gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010276
VERSION     KHUT01010276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggagtga ctgggcgtaa agggcgcgta ggcggcttgt
       61 taagttaatt gttaaattcc accgcccaac ggtgggcaag caattgatac tggcaggcta
      121 gagtgtgaga gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggc tcactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010277
VERSION     KHUT01010277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggttcgt
       61 taagtcagat gtgaaagccc cgggctcaac tcgggaagtg catttgaaac tggcgagctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010278
VERSION     KHUT01010278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           85 g           56 t
ORIGIN      
        1 tacagaaggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttg
       61 taagttaggt gtgaaagtcc cgggcttaac ctggggattg cgcctaatac tgcaaagcta
      121 gagtactgta gaggaaagtg gaatttccag tgtagcagtg aaatgcgtag atattggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010279
VERSION     KHUT01010279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           54 c           84 g           41 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttaggcgtaa agcatctgta ggtggctttt
       61 caagtccgcc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttccga tggagcggtg aaatgcatgg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01010280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010280
VERSION     KHUT01010280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcgaca
       61 caagtcagag gtgaaatccc agagcttaac tctggaactg cctttgaaac tgtgttgcta
      121 gagtactgga ggggagtggg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg ccactctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010281
VERSION     KHUT01010281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           52 c          105 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agagcacgta ggcggcttgt
       61 taagatgggc gtgaaatctc cgggcttacc tcggaggggt cgtccgtgac tggcaggctt
      121 gggtctggga gaggcgagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgtaggcg actcgctggg ccagtaccga cgctgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010282
VERSION     KHUT01010282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           94 g           42 t
ORIGIN      
        1 tacagaggcc ccgagcgcta atcggaatca ctgggcttaa agggtgcgta ggcggattcg
       61 caagcgtctt gtgaaagccc ccggctcaac cggggaactg cggggcgaac tgcgagtctt
      121 gaggcaggta tgggtcgctg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg agcgactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010283
VERSION     KHUT01010283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           53 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ttcggaatca ctgggcgtaa agcgtgtcta ggcggctatc
       61 caagtctgtt gtgaaagccc ttggctcaac caaggatgtg caatggatac tgggtggctt
      121 gaattgtgga gagatgggcg gaattccgag tgtagcagtg aaatgcgtag atattcggag
      181 gaacaccaga ggcgaaggcg gctcattggc cacatattga cgctaaagca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010284
VERSION     KHUT01010284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           93 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttttg
       61 taagtaagat gtgaaatccc tgggcttaac ctaggaattg cattttagac tatgagactg
      121 gagtacagta gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gccacctgga ctgtaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010285
VERSION     KHUT01010285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctttgatac tggcgagctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010286
VERSION     KHUT01010286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcacgca ggcggcctag
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgaaac tgctaggcta
      121 gagatcgaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010287             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010287
VERSION     KHUT01010287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           83 g           54 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggttg
       61 aaatagtctt gggtaaaagc cggaagctca acttccgcgt ttgcttaaga tacttttcgg
      121 ctagaggggc aaagaggtgc ttggaaccaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 tgggaacacc gaaagcgaag gcagagcact gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01010288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010288
VERSION     KHUT01010288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtccgtc gtgaaagtcc agggctcaac cctggaatgc cgatggatac tgccggacta
      121 gagttcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctggg acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010289             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010289
VERSION     KHUT01010289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           48 c           78 g           54 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggttt
       61 agaaagtttt gggtaaaagc cagaagctca acttctgagt ttgctcaaaa tacttctcaa
      121 ctagaggggc aaagaggtcc ttggaacgaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 tcggaacacc gaaagcgaag gcagaggact gggtgccacc tgacactact aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01010290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010290
VERSION     KHUT01010290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatg
       61 taagtgagtt gtgaaagccc tgggcttaac ctaggaactg caacttatac tgtatagcta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010291
VERSION     KHUT01010291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctccg
       61 caagttgggt gtgaaatccc tcggcttaac cgaggaagtg cgctcaaaac tacagagctt
      121 gagtaccgga gagggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg accacctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010292
VERSION     KHUT01010292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctat
       61 taagtgacgg gtgaaatccc tcggcttaac cgaggaactg cctgtcagac tggaaggctt
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010293
VERSION     KHUT01010293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           88 g           56 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccta
       61 caagtcaggg gtgaaatacc ggagcttaac ttcggaattg cttttgatac tgtagggcta
      121 gagtatgtta gaggatggcg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg acataactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010294
VERSION     KHUT01010294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggagcgta ggcggcccgt
       61 taagtcagag gtgaaagacg gtagctcaac tatcgcagtg cctttgatac tgacgggctt
      121 gaatacagat gaggtgggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gctcactaag ctgttattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010295
VERSION     KHUT01010295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           82 g           53 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggtatgc
       61 caagtcaact gtttaattct ccggcctaac cggagtccag cggtagatac tagcgtacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatacctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010296
VERSION     KHUT01010296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           89 g           48 t
ORIGIN      
        1 tacgaactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggtggcatgg
       61 aaagtagggt gtgaaatgcc ttggctcaac caaggcacag cgctctaaac tactttgctt
      121 gagtgagtca ggggtgcacg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccggc ggcgaaagcg gtgcactggg gcttaactga cactgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010297             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010297
VERSION     KHUT01010297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           49 c           78 g           56 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggttgttt
       61 acaaagtcag aggtgaaatc tccgagctca actcggaatc tgtcctttga aacttgtaga
      121 ctagaggggc aaagaggaag ctggaacaaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 ttggaacacc aatagcgtag gcaggcttct gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01010298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010298
VERSION     KHUT01010298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcag
       61 tcagtctggt gtgaaatctc ggggctcaac cccgagcggt catcggatac tgctgggcta
      121 gaatccggaa gaggcaagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gcttgctggt acggtattga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010299
VERSION     KHUT01010299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           81 g           57 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggatatt
       61 taagtcggat gtgaaatccc tgggcttaac ctgggaatcg cattcgatac tggatatctt
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga ccaatactga cactaaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010300
VERSION     KHUT01010300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           89 g           50 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaactg cttggcatac tggatgtctt
      121 gagcggtcta ggggcgagcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgcctat ggtgaaaaca gctcgctggg gacctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010301
VERSION     KHUT01010301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           82 g           58 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggttgtcatt
       61 caagtcagag gtgaaatccc ggagcttaac tccggaattg cctttgaaac tgaatggcta
      121 gagagtgtta gaggtaagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacatcaat ggcgaaggca gcttactggg tcacatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010302
VERSION     KHUT01010302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctggt gttaaagccc ggagctcaac tccggcatgg cattggaaac tgggcggcta
      121 gagtgtggca gaggtatcgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgatactggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01010303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010303
VERSION     KHUT01010303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          101 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggttcgg
       61 taagtcggtg gtgaaaaccc ggggctcaac cccgggccgt ccgtcgatac tgccggacta
      121 gaggcaggta ggggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctctctgga cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01010304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010304
VERSION     KHUT01010304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 gacagggggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggtcccg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaactg cgtctgatac tgcggggctt
      121 gaggacggga ggagaaagtg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg actttctggt ccgttcctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010305
VERSION     KHUT01010305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtccga
       61 taagtcggct gtgaaagtca agggctcaac ccttgaatgc cggccgatac tgtcgggcta
      121 gagtccggaa gaggtgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacgccaat ggtgaagaca gctcactggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010306
VERSION     KHUT01010306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          107 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcttgg
       61 agagcccggc gtgaaagccc ccggcttaac cgggggaggc cgtcggggac cttcaggctg
      121 gaggccggta ggggagagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccggtcctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01010307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010307
VERSION     KHUT01010307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggtggatatt
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tgggtatctt
      121 gagttcggga gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggc ccgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010308
VERSION     KHUT01010308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           81 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggttgt
       61 taagtcagtg gtgaaagatt cgagctcaac tcggaaactg ccattgatac tgacaatctt
      121 gaatatagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttgctaaa ctattattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010309
VERSION     KHUT01010309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           66 c          106 g           32 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccga
       61 caggtccgtg gtgaaagctt ccggctcaac cggaaacccg ccgcggatac cggcggtctg
      121 gaggcgggca ggggcgcgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggc ggcgaaagcg gcgcgctggg cccgtcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010310
VERSION     KHUT01010310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtatgta ggcggcctgt
       61 caagtcagct gtgaaatccc tgggctcaac ccaggacgtg cagttgatac tgatgggctt
      121 gaatatggga gaggatagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctggc ccaatattga cgctgagata cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010311
VERSION     KHUT01010311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           85 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgtgta ggcggtgcat
       61 taagtcaatt gtcaaatctc tcagcctaac tgagagtcgg ctgttgaaac tgatgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatacctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010312
VERSION     KHUT01010312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttga
       61 caagtcgatt gtgaaatccc cgggcttaac ctgggaactg cagtcgaaac tgtcttgcta
      121 gagtatggga gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acctcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010313
VERSION     KHUT01010313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcgg
       61 taagtgaggt gtgaaatccc tgagctcaac tcaggaactg cacttcaaac tgccggtctc
      121 gagtgtcgga gagggtggtg gaattcttgg tgtagaggtg aaattcgttg atatcatgag
      181 gaacaccagt ggcgaaggcg gccacctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010314
VERSION     KHUT01010314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           91 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggttcgt
       61 taagtcgctt gtgaaagccc ccggctcaac cggggaactg caagcgatac tggcgagcta
      121 gagtgtcgga ggggatagtg gaatttccag tgtaggggtg aaatccgtag atattggaag
      181 gaacacctgt ggcgaaggcg gctatctgga cgattactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010315
VERSION     KHUT01010315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggccaaa
       61 taagtctggt gtgaaagccc ggggcttaac cccggaagtg cactggatac tgtttggcta
      121 gaggacgaga gaggaaagtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg actttctggc tcgtttctga cgctcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010316
VERSION     KHUT01010316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           84 g           55 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggcggactta
       61 taagtcagat gtgaaatccc agggctcaac cctggaagtg catttgatac tgcaagtctt
      121 gagtgtggta gaggttactg gaattgttgg tgtagtggtg aaatacgtag atatcaacag
      181 gaataccgga ggcgaaggcg agtaactggg ccaacactga cgctgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010317
VERSION     KHUT01010317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           49 c           80 g           49 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaataa ctgggcttaa agcgtgcgca ggcggtcttg
       61 aaagtcagaa gtgaaagcct ggggcttaac cccagaattg cttttgaaac tccaagacta
      121 gaatcccgga gaggttagca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccaat ggcgaaggca gctaactgga cgggcattga cgctcaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010318
VERSION     KHUT01010318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           60 c          111 g           37 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgtgcgca ggcggctgcg
       61 ttagcctggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac ggcgcagctt
      121 gagagcggca ggggccggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccggctggg ccgactctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010319
VERSION     KHUT01010319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           83 g           50 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcacgta ggcggattga
       61 caagtcaatt gtgaaagccc taggctcaac ctaggaacag cagttgaaac tgtctttctt
      121 gaatatctga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgcctggc agaatattga cgctgaggtg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010320
VERSION     KHUT01010320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcgggtatt
       61 taagtcagat gtgaaaactt ggggctcaac cgcaagattg catttgaaac tggatacctt
      121 gagggtaaga gagggcagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actgcctggc ttacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010321
VERSION     KHUT01010321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           90 g           48 t
ORIGIN      
        1 tacagagggc tcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcgggtgtg
       61 caggcatgtt gtgaaatccc atggctcaac catggaattg cttcgtgaac cgcacatctt
      121 gaggctggta gaggcagttg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgccgaa ggtgaagaca agctgctggg ccagtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010322
VERSION     KHUT01010322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           62 c          109 g           38 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtcgtg
       61 tgtgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggagac tgcacgactt
      121 gagggcggta ggggctggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccagctggg ccgtccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010323
VERSION     KHUT01010323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccttg
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tgcagggctt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gccaactgga cgattactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010324
VERSION     KHUT01010324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           99 g           39 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ctcggtatca ctgggcttaa agggcgcgta ggcggtgcgg
       61 cacggtccgc gtgaaatccc tcggctcaac cgaggaattg cgcgggcaac gaccacgctg
      121 gacggcatca ggggaagatg gaactcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg atcttctggg gtgcctgtga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010325
VERSION     KHUT01010325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          102 g           40 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtcctt
       61 caggtcatct gtcaaagcac ccggctcaac cgggttgtcg cgggtgaaac cgggggactt
      121 gagggcggta gagggtggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacccgt agcgaaggcg gccacctggg ccgaccctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010326
VERSION     KHUT01010326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggctggc
       61 taagtccgat gtgaaatctc ccggctcaac tgggaaacgg catcggaaac tgaccggctt
      121 gaaggtggga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggc ccactcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010327
VERSION     KHUT01010327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           78 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggagcgta ggcgggattg
       61 taagtcagtg gtgaaagccc gtagctcaac tacggaactg ccattgatac tgcagaccta
      121 gagtacactt gaagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcactaaa ttgtaactga cgctgaggct cgatagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010328
VERSION     KHUT01010328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtatgg
       61 taagttatct gtgaaatccc cgggcttaac ctgggcaggt cagataagac tgcgatactt
      121 gagtgaggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaacactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010329
VERSION     KHUT01010329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacggggggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttag
       61 caagtggtct gtgaaattcc tgggcttaac ctgggcaggt cagatgagac tgctagactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc cagatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010330
VERSION     KHUT01010330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacagaggtc tcaaccgttg ttcggattta ttgggcgtaa agggtgcgta ggtggtcgtg
       61 taagtcgggt gtgaaatcct agggctcaac cctggaactg cactcgatac tgcacggctt
      121 gagcgctgga aaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg agtctctgga cagatgctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010331
VERSION     KHUT01010331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggatgct
       61 taagtcatat gtgaaagccc cgggctcaac ctgggaattg catctgatac tgggcgtcta
      121 gagtatggta gaggatggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gccgtctggg ccaatactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010332
VERSION     KHUT01010332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           93 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 taagtctgat gtgaaaggct ggggctcaac ctcagtatgg cattggatac tgccttgcta
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010333
VERSION     KHUT01010333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggctgtaagt
       61 caagtcagcg gtgaaaagtc ggggctaaac cttgagggtg ccgttgaaac tgatttacta
      121 gaatgtggat gatgccggcg gaatgtgtca agtagcggtg aaatgcatag atatgacaca
      181 gaaggccgat tgcgaaggca gctggcaaag gcacgattga cgctgaggca cgaaagtgcg
      241 gggatcgaac agg
//
LOCUS       KHUT01010334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010334
VERSION     KHUT01010334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           35 c           80 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttaaa
       61 taagtcagtg gtgaaagttt gcagcttaac tgtaaaattg ccattgatac tgtataacta
      121 gagtgcagtt gaggtggttg gaatgagtag tgtagcggtg aaatgcttag atattactca
      181 gaacaccgat tgcgaaggca gatcactaag ctgtaactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010335
VERSION     KHUT01010335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c          101 g           44 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattca ctgggcgtac agggtgtgta ggcggtttgg
       61 taagcctttt gtgaaatctt cgggcctaac ccggaaagag cagaggggac tgccaggcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgtatctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010336
VERSION     KHUT01010336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           73 g           60 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggaaaat
       61 taagtcagtg gtgaaagctg gtagctcaac tatcaaattg ccattgaaac tgattttctc
      121 gagtacggtt gaggtagctg gaatgtaaca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccaat tgcgaaggca ggctactaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010337
VERSION     KHUT01010337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta gacggtaagt
       61 taagtttgct gttaaatcct tcggcttaac cgaagatcag cggtgaatac tggcatacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccacatctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010338
VERSION     KHUT01010338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctcgt
       61 taagtctgat gtgaaatctc tgggcttaac ccagaaactg catcggaaac tagcgagcta
      121 gagttcggga gagggtcgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgacctgga ccgacactga cgctaataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010339
VERSION     KHUT01010339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggcccga
       61 taagtctgct gtgaaagtca aaggctcaac ctttgaatgc cagtggatac tgtcgggcta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gctcgctggg acgttactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010340
VERSION     KHUT01010340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          103 g           42 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtac agggagcgta ggcggtttgg
       61 taagcccctc gggaaatctc caggctcaac ctggaaagat cgggggggac tgcctggcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgttcctga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010341
VERSION     KHUT01010341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgaat
       61 taagttggat gtgaaagccc tgggcttaac ctaggaactg catccaagac tgaattactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010342
VERSION     KHUT01010342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgctcgta ggcggccgag
       61 taagttgaat gtgaaagccc tgggctcaac ccaggaagtg cattcaatac tgttcggctt
      121 gagtcccgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccggt ggcgaaggcg accacctgga cggagactga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010343
VERSION     KHUT01010343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           99 g           37 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtccg
       61 caagtcaggg gtgaaagccc ccagctcaac tggggaagtg cctctgatac tgcgggcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010344
VERSION     KHUT01010344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg tcgagcgttg tccggattta ttgggcgtaa agcgcccgca ggcggcttgg
       61 taagtctcgg gtgaaatctc ctggctcaac taggaggggt cttgagaaac tgccaggctt
      121 gaggcatgga gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcttcctggc catgacctga cgctcagggg cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01010345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010345
VERSION     KHUT01010345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           90 g           45 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgagg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac taccttgctc
      121 gaggaagaca gaggagagcg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaggcg gctctctggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010346
VERSION     KHUT01010346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           90 g           44 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattca ctgggcgtaa agcgcacgca ggtggcctga
       61 caggtagggc atgaaatctc ccggcttaac tgggagcagc tgtcctaaac cgtcaggctt
      121 gaggatagga gaggggagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaagcg gctccctggc ctactcctga cactcaagtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010347
VERSION     KHUT01010347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           85 g           57 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttagat gtgaaatacc ggtgcttaac atcggaattg catttaagac tgccttgctt
      121 gagcattaga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg agtctctggc taaatgctga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010348
VERSION     KHUT01010348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           77 g           57 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggccctg
       61 taagtcagtg gtgaaatctt tgggcttaac ccaaaaattg ccattgatac tgcagggctt
      121 gagtacagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010349
VERSION     KHUT01010349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaagaca acagctcaac tgttgcagtg ccattgatac tgaagagctt
      121 gagtacagat gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacacctat tgcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010350
VERSION     KHUT01010350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           54 c           86 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggattca ctgggcttaa agggtgcgta ggcggctcag
       61 aaagtagggt gtgaaagccc tcggctcaac cgaggaactg cgccctaaac tactgggctt
      121 gaggaacgca gaggtaagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacaccagg agcgaaagcg gcttactggg cgttttctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010351
VERSION     KHUT01010351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           84 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggagtta ttgggcgtaa agggcgcgta ggcggataga
       61 aaagtctgat ctgaaatacc gaggctcaac ctcggtgctg gattggaaac tctttgtcta
      121 gagggttggt ggagaaaacg gaattccaag tgtagcggtg aaatgcgtag atatttggaa
      181 gaacacctgt ggcgaaagcg gttttctaac taacacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01010352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010352
VERSION     KHUT01010352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           82 g           54 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggtcatg
       61 ttagtcttct gttaaagctc tcggctcaac cgggaacatg cagaggaaac ggcatgacta
      121 gaggatgtga gagatgtgcg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gcacattggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010353
VERSION     KHUT01010353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           82 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ctgggcgtaa agagcgcgta ggcggttcaa
       61 taagtccgat gtgaaatccc tggactcaat ccaggaactg catcggatac tgttgaactt
      121 gagtatagga gaggacagcg gaattctagg tgtagaggtg aaattcgtag atatctagag
      181 gaacaacaga agcgaaggcg gctgtctgga ctattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010354
VERSION     KHUT01010354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           62 c          112 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtccgt
       61 taagcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac tggcggactt
      121 gaggacggca ggggctggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccagctggg ccggtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010355
VERSION     KHUT01010355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           81 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaatga
       61 taagtcagat gtgaaatccc ggggctcaac ctcggaacag catttgaaac tgtcattcta
      121 gaggatagaa ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatt ttagacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01010356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010356
VERSION     KHUT01010356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           51 c           81 g           48 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgtcttgt
       61 cacgtctggg gacaaatcgc agggctcaac cctgcgccct cctcggatac gagcaggata
      121 gagttattga gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaagca ggatgctagc aatatactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01010357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010357
VERSION     KHUT01010357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccaga
       61 caggtctgct gtgaaaactc gaggctcaac ctcgagatgt cggtggaaac cgtctggcta
      121 gagtgcggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggctaaggcg gctctctggt acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010358
VERSION     KHUT01010358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcacca
       61 caagtcactt gtgaaatctg aaagcttaac tttcagcggc caagtgatac tgtggagcta
      121 gagtgcagaa ggggcaactg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010359
VERSION     KHUT01010359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggactta ctgggcgtaa agcgcgcgca ggtggacatg
       61 tatgtgccgt gtgaaagcgc cgtccttacg atggtgaggt catggtagac agcatgtctt
      121 gaggctcgaa gaggttgatg gaattccggg tggagcggtg aaatgcgtag atatccggag
      181 gaacaccagg ggcgaaggcg gtcaactggt cgagttctga cactgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010360
VERSION     KHUT01010360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcagg
       61 taagtctgat gtgaaatctc tgagctcaac tcagaaatgg cattggatac tatctggcta
      121 gagggtcgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga cgactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010361
VERSION     KHUT01010361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgta
       61 taagttaggt gtgaaatccc cgggcttaac ctgggaatgg catttaatac tgtatgacta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ctcatactga cgttgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010362
VERSION     KHUT01010362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttata
       61 taagtgaggt gtgaaagccc cgggcttaac ctgggaactg catcttatac tgtgtaactg
      121 gagtgcgata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ttgacactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010363
VERSION     KHUT01010363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           88 g           53 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ctgggcgtaa agggtgtgta ggtggtcgtg
       61 ttagtctctc gttaaattct tcggcttaac cgggggcatg cgggggaaac ggcacgactt
      121 gaggatgcga ggggcctgtg gaactcatgg agtaggggta atatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca gcaggctggc gcattcctga cactgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010364
VERSION     KHUT01010364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           85 g           49 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggtggtcgtg
       61 tgtgtcagat gtgaaatccc ggggcttaac tccggaactg cgtctgaaac tgcacgacta
      121 gagcattgga gagggtagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg actacctgga caattgctga cactcaggca cgaaagcatg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01010365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010365
VERSION     KHUT01010365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           94 g           47 t
ORIGIN      
        1 gacgaaccaa gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtctgt
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaattg cctcggatac tgatgggctc
      121 gaagggggta ggggttgcgg gaacagccag tggagcggtg aaatgcgttg atattggctg
      181 gaacgccggt gccgaaaggg cgcaactgga cctctcttga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010366
VERSION     KHUT01010366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           95 g           44 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctcgg
       61 aaagtcggtt gtgaaatccc agggctcaac cctgggactg cattcgatac ttccgagctc
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01010367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010367
VERSION     KHUT01010367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgcg
       61 taagtcagac gtgaaatccc caggcttaac ctgggaactg cgtctgatac tgcatggctt
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010368
VERSION     KHUT01010368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          102 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcaccc
       61 caagtacctt ctgaaatcca cctgctcaac gggtggggtg gagggtagac tggggagcta
      121 gagagaggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg cctctactga cgccgaggcg cgaaggctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010369
VERSION     KHUT01010369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           84 g           54 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatttg
       61 caagtcagaa gtgaaatccc cgggcttaac ccgggaattg cttttgaaac tgcaaatcta
      121 gaatccggga gaggtcagca gaattcccag tgtagaggtg aaattcgcag atattgggag
      181 gaataccagt ggcgtaggcg gctgactgga ccggttttga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010370
VERSION     KHUT01010370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           97 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaatgg ccttggatac tggcgggctc
      121 gaagggggta ggggcagctg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctctcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010371
VERSION     KHUT01010371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           40 c           91 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgat
       61 taagttatct gtgaaattcc tgggcttaac ctgggcgagt cagatgatac tgattgactt
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010372
VERSION     KHUT01010372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           48 c          104 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggacggc
       61 ttagtcgggc gtgaaagtcc tgggctcaac ctggggacgg cgttcgatac gggtggtctg
      121 gagttgggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010373
VERSION     KHUT01010373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggatgaa
       61 aaagtctgtg gtgaaagccc ccggctcaac cggggaacag ccgtggatac ttttcgtctg
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010374
VERSION     KHUT01010374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           93 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggctacg
       61 caagtcggat gtgaaatccc gaggctcaac ctcaggactg cacccgatac tgcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010375
VERSION     KHUT01010375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggttcca
       61 taagtctgct gtgaaagtcg gaggctcaac ctccgaaagc cggtggatac tgtggaacta
      121 gaatgcggaa gaggcaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gcttgctggg acgttattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010376
VERSION     KHUT01010376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           84 g           58 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcaggg
       61 taagttaggt gtgaaatatc gaggctcaac ttcgatactg cacttgatac tgccttgctc
      121 gagtgctaga gaagaagctg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacgccagt ggcgaaagcg agcttctaga tagttactga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010377
VERSION     KHUT01010377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           82 g           56 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggattta ttgggcgtaa agggtgcgca gacggtatgt
       61 taagtcacct gttaaattct ctggcctaac cagaggcatg cgggtgaaac tggcaaacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010378
VERSION     KHUT01010378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           97 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg ccttggatac tggcggcctt
      121 gagggaggta ggggtatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010379
VERSION     KHUT01010379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          100 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcagt
       61 caagtcgggg gtgaaatccc acggcccaac cgtggaaggg cccccgatac tgggtgcctg
      121 gagggaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010380
VERSION     KHUT01010380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           89 g           44 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc ttggcttaac caaggaattg cgcccaaaac taccgtgctt
      121 gagggagaca gaggtgagcg gaactcaggg tggagcggtg aaatgcgttg atatcctgag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcatctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010381
VERSION     KHUT01010381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtgaca
       61 taagtaacag gtgaaagtcc cgagcttaac ttggggattg cctgttaaac tgtgtcgcta
      121 gagtgttgta gaggagagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctctctgga caacaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010382
VERSION     KHUT01010382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c           98 g           40 t
ORIGIN      
        1 gacgaaccgc ccgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgac
       61 ctggtcgggg gtgaaatctt tcagcttaac tggaaaacag cccccgatac cggccgtctg
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010383
VERSION     KHUT01010383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c           95 g           55 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgca ggcggttcgt
       61 taagtttggg gtgaaatctc ccggctcaac tgggaggggt tctcagatac tggcggactt
      121 gagggaggta gaggagagta gaattctcgg tgtagtggtg atatgcgtag atatcgagag
      181 gaataccagt ggcgaaggcg gctctctgga ccttacctga cgctaagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010384
VERSION     KHUT01010384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          102 g           44 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggtacga
       61 taagtctgct gtgaaagcgc tcggcttaac cgggcgaggt cggtggatac tgtcgcactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010385
VERSION     KHUT01010385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggatctt
       61 taagtcagtg gtgaaagccc cgggctcaac ccgggaactg ccattgatac tattgatctt
      121 gaattcagtt gaggtgggcg gaatgtgtcg tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010386
VERSION     KHUT01010386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           94 g           42 t
ORIGIN      
        1 gacgaaggtc ccaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcagat gtgaaagccc cgggcttaac ccgggaatgg catccgatac tcccgcgctc
      121 gaggactgga gaggagtctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg agactctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcgaag ggg
//
LOCUS       KHUT01010387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010387
VERSION     KHUT01010387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           98 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtgggagcg
       61 tcagtccggt gggaaagccc acggctcaac cgtggaagtt cgctggaaac tgcgcttctt
      121 gagggtgata gggggaagtg gaattgctgg tgtagcggtg aaatgcgtag atatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctgga tcacccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010388
VERSION     KHUT01010388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           85 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggaagga
       61 taagttagaa gtgaaatacc ctggctcaac ctgggaaatg cttttaagac tgttcatcta
      121 gagtttgtca gaggggggtg gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaacaccgat ggcgaaggca gccccctggg ataaaactga cgctcaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010389
VERSION     KHUT01010389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           97 g           49 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccga
       61 taagttagat gtgaaagtcc cgttttcaag atggggagtg catttgatac tgacgggctt
      121 gagtgcagga gaggtaagcg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010390
VERSION     KHUT01010390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          101 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaaagt
       61 taagcgtgac ctgaaagccc ccggctcaac cggggagggc ggctgcgaac tggctttctt
      121 gagggcgata gaggcgagcg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccaat ggcgaaggca gctcgctggg tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010391
VERSION     KHUT01010391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          101 g           48 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcat
       61 cgcgtctgcc gtgaaaaccc actgctcaac ggtgggcttg cggttgatac gggtgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010392
VERSION     KHUT01010392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          103 g           41 t
ORIGIN      
        1 tacggaggtg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggggagg
       61 taagtcgggt gtgaaatccc tcggcccaac cgaggaaccg cgcccgagac tgcccttctt
      121 gagtcccgga gaggaaggtg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010393             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010393
VERSION     KHUT01010393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           30 c           71 g           74 t
ORIGIN      
        1 tacagggggg actagtgtta ttcggagtga ctgggcgtaa agggcatgta ggcggtttat
       61 tatgttaaaa gtcaaagacc agaattgtta ttttggaata tcttttaaaa cggataaact
      121 agagttagaa agaggatagt ggaatttcta gtgtagagat aaaattcgta aatattagaa
      181 ggaactccta ttggcgaagg ctactatctg gttctattta ctgacgctga ggtgctaaag
      241 catggggatc aaacggg
//
LOCUS       KHUT01010394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010394
VERSION     KHUT01010394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 taagtcgaat gtgaaagccc tcggcttaac cgaggaagtg cattcgatac tgccgagctt
      121 gagtaccgga gaggaaggtg gaacacccgg tgtagaggtg aaattcgtag atatcgggta
      181 gaacaccagt ggcgaaggcg accttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010395             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010395
VERSION     KHUT01010395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           80 g           55 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggttt
       61 aataagtctt gggtaaaagc cggaagctca acttccgcgt ttgcttaaga tactattaga
      121 ctagaggggc aaagaggtgc ttggaacgaa tggtgtagta gtgaaatgcg ttgatatcat
      181 tcggaacacc gaaagcgaag gcagagcact gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01010396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010396
VERSION     KHUT01010396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggatact
       61 taagttggat gtgaaagccc tgggcttaac ctaggaatgg catacaaaac tgggtatctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010397             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010397
VERSION     KHUT01010397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           32 c           72 g           66 t
ORIGIN      
        1 tacgagaggg gcaagcgtta ttcggaatga ttgggcgtaa agagcacgta ggcggtttat
       61 aatatatgga ataccaagct caaggcttaa ccttgaagag tattttaaaa atagtcataa
      121 gcttgagtaa gtaataggag aatagaattc ctggagtaga ggtaaaattt gttgatttca
      181 ggaggaatac caaaagcgaa ggcaattctc ttgtacttta ctgacgttga ggtgcgaaag
      241 cgtggggatc aaaaggg
//
LOCUS       KHUT01010398             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010398
VERSION     KHUT01010398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           81 g           53 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggaatca ttgggcgtaa agcgtaccga taggtggttt
       61 tgaaagtttt gggtaaaagc cagaagctca acttctgagt ttgctcaaaa tactgcaaga
      121 ctagaggggc aaagaggtgc ttggaacgaa cggtgtagta gtgaaatgcg ttgatatcgt
      181 tcggaacacc gaaagcgaag gcagagcact gggtgccacc tgacactgct aggacgaaag
      241 cgtggggagc gaatggg
//
LOCUS       KHUT01010399             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010399
VERSION     KHUT01010399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           84 g           55 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggcatga
       61 taagttagcg gtgggaaatc gaggctcaac ctcgtggaag ctggtaatac tgtcatgctt
      121 gagtatagga gaggttaacg gaactccctg tggagcggta aaatgcgtag atatagggaa
      181 gaacaccaat aacggcgaag gcagttaact attctattac tgacgttgag agcacgaaag
      241 cgtggggagc aaaacgg
//
LOCUS       KHUT01010400             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010400
VERSION     KHUT01010400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c          105 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agagcgtgta ggcggtcctt
       61 tttgtcgccc ttgaaagccc cctgctcaac ggggggaggg agggcgaaac gggaggactc
      121 aatggagtct ggtaggggga ggcggaacgg ccggtggagc ggtgaaatgc gtagagatcg
      181 gccagaacac cagtggcgaa ggcggcctcc tgggccagaa ctgacgctga gacgcgacag
      241 cgtggggagc gaacgga
//
LOCUS       KHUT01010401             257 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010401
VERSION     KHUT01010401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>257
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c          100 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggctcgt
       61 taagttcgat gtgaaagccc tcggctcaac tgaggaatgg cattggaaac tggcgggcta
      121 caaagagtac cggagaggga agcggaattc ctggtgtagc ggtgaaatgc gtagatacca
      181 ggaggaacac cggtggcgaa ggcggcttcc tggccgagta ctgacgctga ggcacgaaag
      241 cgtggggagc gaacggg
//
LOCUS       KHUT01010402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010402
VERSION     KHUT01010402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           43 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggcggcttgt
       61 taagtcagcg gtgaaatcgt ccggcccaac cggacacgtg ccgctgatac tggcgagctg
      121 gagtgcgtga ggggtggggg gaacgagcgg tgtagcggtg aaatgcgtag atatcgctcg
      181 gaaggccaga gccgaagggg ccccactggt acgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010403
VERSION     KHUT01010403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           56 c           89 g           41 t
ORIGIN      
        1 tacagagact gcaagcgtta ctcggattca ctgggcgtaa agggagcgca ggcggaccgg
       61 tgtgtcagat gtgaaacccc agggctcaac cctgggactg cgtctgaaac tgccggtcta
      121 gggactcgga ggggctagtg gaattcctgg tggagcagtg aaatgcgtag atatcaggag
      181 gaacaccaac ggcgaaggca gctagctgga cgagatccga cgctcaggct cgaaagtatg
      241 gggagcaaaa agg
//
LOCUS       KHUT01010404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010404
VERSION     KHUT01010404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           83 g           58 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggtgggg
       61 taagtcagag gtgaaattct aaagcttaac tttggaactg cctttgatac tacttcactt
      121 gtgtacggaa gagggtgatg gaatttcagg tgtagcagtg aaatgcgtag atatctgaaa
      181 gaacaccagt ggcgaaggcg gtcacctggt ccggtacaga cactaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010405
VERSION     KHUT01010405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           95 g           40 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agcgaccgca ggcggctcgc
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaatgg cattcgaaac tggcgagctc
      121 gagtacggga gaggggggtg gaattcctgg tgtaggggtg aaattcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg gccccctggc ccgcaactga cgctcatggt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010406
VERSION     KHUT01010406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgtgta ggcggttgac
       61 aaagagaggc gtgaaattcc cgggcttaac ctgggcattg cgcctgagac tcgttaactg
      121 gagtgtcgga gagggaagta gaatttccgg tgtagcggtg aaatgcgtag agaccggaaa
      181 gaataccggt ggcgaaggcg actttctggc cgatgactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010407
VERSION     KHUT01010407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           82 g           51 t
ORIGIN      
        1 tacgtagggc ccgagcatta tccggaatta ttgggcgtaa agagcgtgta ggtgttccta
       61 caagtcggtc gttaaatctc agagctcaac tctgaggccg cgagcgaaac tgtaggaatt
      121 gaggtttaca ggggcaagca gaatttctgg agtaggggtg aaatccgttg ataccagaag
      181 gaataccaat ggcgaaggca gcttgctggg taacacctga cactgagacg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010408
VERSION     KHUT01010408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           81 g           61 t
ORIGIN      
        1 tacgtagggt cctagcgttg ttcggattca ctgggcgtaa agcgtatgta ggcggccttt
       61 taagtcaggt gtgaaagccc agggctcaac cctggaactg catttgatac tatttggctt
      121 gaatatggta gaggttggta gaatttccgg tgtagtggtg aaatacgtag atatcggaag
      181 gaataccggc ggcgaaggcg gccaactgga ccaatattga cgctgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010409
VERSION     KHUT01010409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           97 g           40 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggattca ctgggcataa agggcgcgta ggcggttccg
       61 aaagtggccc gtgaaatccc ccggctcaac cggggaagtg cggaccaaac ttcggtgcta
      121 gagggtggga ggggagactg gaactctcgg tggagcggtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctggt ccaccactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010410
VERSION     KHUT01010410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           84 g           57 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta gacggtatgt
       61 taagtcaact gttaaattcc tcggcctaac tgagggctcg cggtagaaac tggcgtactt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010411
VERSION     KHUT01010411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 gacggaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgtctgta ggcggacaag
       61 ttagtctggg gtaaaaggcg gaggctcaac ctccgttccg tcctggaaac tgcttgtctc
      121 gagcaactca gaggcacctg gaatgctgtg agtaggggta aaatccgttg atccacagtg
      181 gaacgctaaa agcgaaagca gggtgctggg ggtttgctga cgctgagaga cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010412
VERSION     KHUT01010412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           92 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttatct gtgaaagccc tgggcttaac ctgggaaggc cagataagac tgttagactc
      121 gagtacggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg gctgtctggc ctgatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010413
VERSION     KHUT01010413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           76 g           57 t
ORIGIN      
        1 gacgaaggac ccgagcgtta tccggattca ttgggtttaa agggtgcgta ggtggattgg
       61 taagtcagtg gtgaaagccc caggctcaac ttgggaactg ccattgatac tgctaatctt
      121 gagtgcatat gatgtgggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgcaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010414
VERSION     KHUT01010414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcagg
       61 taagtcgaat gtgaaagccc agggctcaac cttggaagtg catccgaaac tacttgactc
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accgcctgga cgaagattga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010415
VERSION     KHUT01010415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           97 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtccct
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccggatac tgaggggcta
      121 gagtgcggga ggggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgcaaacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010416
VERSION     KHUT01010416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           92 g           41 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccgat
       61 caagttccgc gtgaaatctc acggctcaac cgtgagcagc cgtggaaaac tggtcggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010417
VERSION     KHUT01010417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttc
       61 caagacagat gtgaaatccc cgggctcaac ctgggaatgg catttgtgac tggaaggcta
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010418
VERSION     KHUT01010418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           92 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtccgt
       61 taagtcggat gtgaaatctc caagctcaac ttggaaactg catccgatac tggcggactt
      121 gaggacggta gaggtcggtg gaatgacggg tgtaccggtg aaatggacag atatccgtcg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgttcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01010419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010419
VERSION     KHUT01010419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacagagggc tcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggatgtg
       61 caggcatgtt gtgaaatccc acggctcaac cgtggaattg cttcgtgaac cgcacatctt
      121 gaggcaagta agggcggtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccgaa ggagaaatca ggccgctggg cttgttctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010420
VERSION     KHUT01010420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           89 g           50 t
ORIGIN      
        1 tacgaaccgg acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggcgcgg
       61 taagtgaggt gtgaaagccc acggctcaac cgtggaactg cgcttcatac tgccgtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010421
VERSION     KHUT01010421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           93 g           42 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattca ttgggcgtaa agcgcgtgta ggcggccgta
       61 caggtccggt gtgaaaactc gaggctcaac ctcgagaggt catcggaaac cgtacggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010422
VERSION     KHUT01010422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           82 g           58 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgtgta ggcgggttag
       61 taagtcgaat gtgaaattcc aaagctcaac tttgggactg catttgaaac tactagtctg
      121 gaggattggc ggagaaaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg attttctagc ttatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01010423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010423
VERSION     KHUT01010423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggccttg
       61 taagtcgaat gtgaaagccc ccggcttaac tggggagggt cattcgatac tgctaggctc
      121 gaagacagga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010424
VERSION     KHUT01010424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgcgta ggcggttcgt
       61 taagtcggtt gtgaaagccc tgggctcaac ctgggaattg cagtcgatac tgatggacta
      121 gagaacggta gagggaggcg gaactccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccgat ggcgaaggca acctcctggg cctgttctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010425
VERSION     KHUT01010425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctcg
       61 tttgtcaggg gtgaaagcct tgggcttaac tcaagaagtg cccctgaaac ggcgagacta
      121 gagtgctgga gaggtcggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gccgactgga cagtgactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010426
VERSION     KHUT01010426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          105 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggcccag
       61 cgcgttggcg gtgccagctc ttcgcttaac gaagagagtg ccggcaagac ggctgggctt
      121 gagggcctca gagggacctg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaataccaaa ggcgaaggca gggtcctggg aggcaactga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010427
VERSION     KHUT01010427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           91 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtctgc
       61 atagtttaga gtgacagtgc tcggcttaac tgagtaagtg ctttgaatac tggcagactt
      121 gagggcttca gagggacatg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgaaggca gtgtcctggg aagtacctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010428
VERSION     KHUT01010428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggtgcta
       61 taagttcggt gtgaaatctc ccggctcaac tgggaggggt cgccgaagac tgtagtgctc
      121 gaacatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccattgttga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010429
VERSION     KHUT01010429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcatgca ggcggtcctg
       61 taagtttgaa gtgacagttt ccggctcaac cgggaaagtg ctttgaaaac tgcgggactt
      121 gagggcttca gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccgaa ggcgaaggca gtgtcctggg gagtacctga cgctcagatg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010430
VERSION     KHUT01010430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcctta
       61 gaagtcaggc gtgaaagccc cgggctcaac ctgggaatag cgtttgatac tctatggctt
      121 gagttcggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010431
VERSION     KHUT01010431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           93 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcgga
       61 taagtgtggt gtgaaatctc ccggctcaac tgggagggtg cgctacaaac tgtctggcta
      121 gagtgcggga tgggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga ccgtaactga cgctgaaaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010432
VERSION     KHUT01010432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           95 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtgggtccg
       61 caagtcgggc gtgaaatccc tgggctcaac ccgggaactg cgctcgaaac tgcagatctt
      121 gagtgccgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggctactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010433
VERSION     KHUT01010433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           84 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggtttgg
       61 aaagtttctt gtgaaagctc ccggctcaac tgggagaggc caaggaaaac taccaggcta
      121 gagtgtggaa gaggaagcta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctggt ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010434
VERSION     KHUT01010434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagtcaggg gtgaaatgcc aaggcttaac cttggaactg cctttgaaac tgctgagctg
      121 gagtgtcgga ggggatagcg gaattcctaa tgtagtggtg aaatacgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010435
VERSION     KHUT01010435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcttag
       61 caagtcagag gtgaaagcct ggagctcaac tccagaattg cctttgaaac tgctaagcta
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010436
VERSION     KHUT01010436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          104 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggagtta ttgggcgtaa agcgcgcgca ggcggttgga
       61 taagtccatg gtgaaagcgc ctggctcaac taggcgagtg ccgtggggac tgttcagctg
      121 gagtggagca gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gctcgctggg ctttgactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010437
VERSION     KHUT01010437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 caagtctgct gtgaaagccc cgggctcaac ctgggaactg cagtggaaac tagcaagcta
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccccctggg ttaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010438
VERSION     KHUT01010438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           50 c          104 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggagag
       61 caagtctgct gtgaaagccc ggagctcaac tccggagggt cggcggagac tgtttttctt
      121 gagggaggca gggagaagtg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg cctttcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010439
VERSION     KHUT01010439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           81 g           53 t
ORIGIN      
        1 tacgagtggt gcaaacgtta ttcggaataa ttgggcgtaa agggtgtgta gacggcgtat
       61 taagtcaact gttaaattct ccggcctaac cggaggcaag cggtagaaac tgatatgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttagt ccattcctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010440
VERSION     KHUT01010440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           53 c          107 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggtggttgct
       61 taagtccgct gtgaaatctc ccggctcaac tgggagggtg cggtggagac tgggcgactg
      121 gagggaccga gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ggtaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010441
VERSION     KHUT01010441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtctgat gtgaaagccc tgggctcaac ctgggaattg cattggatac tggcgagcta
      121 gagtgcggga gaggatggcg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacatccgt ggcgaaggcg gccatctgga ccagcactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010442
VERSION     KHUT01010442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agggtgtgca ggcgggagtg
       61 caagtggtgt atgaaaacgt ccggcttaac cgggcgctgt tatgccagac tgcagttcta
      121 gaggacgaga gaggggggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gccccctggc tcgtacctga cgctcagcca cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010443
VERSION     KHUT01010443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           92 g           44 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctga
       61 caagtcgggt gtgaaatccc ggagctcaac tccggaactg catccgatac tgtcaggctc
      121 gagtctcgga gaggacggcg gaatttccgg tgtagaggtg aaattcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gccgtctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010444
VERSION     KHUT01010444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           95 g           50 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ctcggattta ctgggtgtaa agggcaagta ggcggtcctg
       61 taagccttgc ttgaaaggct ctggctcaac cagagtacgt ggcaggggac tgcaggactt
      121 gaggacggaa gaggcaaacg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtttgctggt ccgtttctga cgctgaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01010445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010445
VERSION     KHUT01010445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           99 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcgggagag
       61 gcagtccggt gtgaaagccc tcggctcaac cgaggaaggt cgctggaaac tcctcttctt
      121 gagggtggca gagggaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gcttcctggg ccaccactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010446
VERSION     KHUT01010446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           96 g           43 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctatc
       61 cacgtcggat gtgaaagctc agggctcaac cctgagaggc cattcgatac gggatagcta
      121 gaggcagcta ggggaaggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctggg gctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01010447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010447
VERSION     KHUT01010447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggctggc
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaattg cacccgatac tggtcggcta
      121 gagtccggga ggggatagcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga ccggaactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01010448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010448
VERSION     KHUT01010448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcttct
       61 caagtcgggg gtgaaatcgt tccgcttaac gggacaatag cccccgatac tgggaagctg
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctcttctga cgctaaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010449
VERSION     KHUT01010449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggtggtttgt
       61 taagtcagac gtgaaagccc caggctcaac ctgggaatag cgtttgaaac tggcaggcta
      121 gagattcaga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacatcagt ggcgaaggcg gctcactggc tgaatactga cactaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010450
VERSION     KHUT01010450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggccgcttgg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tttcaagctt
      121 gagttctgga gagggaagcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010451
VERSION     KHUT01010451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           88 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggtggtttgt
       61 taagtcggat gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac taccaagcta
      121 gaggacggca gaggatggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggc ggcgaaggcg gccatctggg ccgttcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010452
VERSION     KHUT01010452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggaatca ctgggcttaa agcgtacgta ggcggatatg
       61 caggcgtcgt gtgaaagccc tcggctcaac cggggaactg cacggcgaac cgcatgtctt
      121 gagtcaagta ggggctgtcg gaacgctagg tggagtggtg aaatgcgttg atatctagcg
      181 gaacgccgaa ggtgaagaca ggcagctggg cttgtactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010453
VERSION     KHUT01010453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgctatg
       61 taagtcggat gtgaaagccc ttggcttaac caaggaagtg cacccgatac tggatagctt
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgataactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010454
VERSION     KHUT01010454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           91 g           54 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatta ctgggcttaa agggtgtgta ggcggctgat
       61 taagtagggt gtgaaaggct ccggcctaac cggagcattg cgctctaaac tgatcggctt
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttaactga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010455
VERSION     KHUT01010455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgcgca ggcggtccct
       61 taagtctgat gtgaaatctt gcggctcaac cgcaagaggt cattggaaac tgggggactt
      121 gagtgcagga gagggaagcg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgtaactga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010456
VERSION     KHUT01010456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           80 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggctgaaaga
       61 taagtcaggt gtgaaatccc ggggctcaac ctcggaacag catttgaaac tatttttcta
      121 gaggctagat ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctaat ttagacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01010457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010457
VERSION     KHUT01010457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           98 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgac
       61 taagtcggac gtgaaattcc ttggcttaac cagggaactg cgtccgagac tggacggctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggaattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010458
VERSION     KHUT01010458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           89 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggccatg
       61 taagttagat gtgaaaggcc tgggcttaac ctgggaactg cgtataatac tgtgtggcta
      121 gagtactgta gagggtagta gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaataccggt ggcgaaggcg actatctgga cagctactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010459
VERSION     KHUT01010459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatcga
       61 aaagtcaggc gtgaaatccc tgggctcaac ctaggaactg cacttgaaac tctcggtctt
      121 gagtaatgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga catttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010460
VERSION     KHUT01010460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggccttg
       61 tcagtcctgt gtgaaaggct ccggcttaac cggagagggt catgggaaac tgcagggctt
      121 gagtgccgga gagggtagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg actacctgga cggtaactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01010461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010461
VERSION     KHUT01010461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccatg
       61 tatgtcaggt gtgaaatgcc acggcttaac cgaggcactg catttgaaac tgcatggctt
      121 gagtgcagga gagggaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttcctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010462
VERSION     KHUT01010462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           98 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgatg
       61 tgagtgcgat gtgaaagccc tgggcttaac ctgggaagtg catcgtaaac tgcattgctg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010463
VERSION     KHUT01010463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 tacgaagggc ccgagcgttg tgcggattca ctgggcttaa agggtgcgta ggcgggagcg
       61 taagtgtttt gtgaaagccc acggctcaac cgtggaactg caggacatac tgcgctcctt
      121 gaggtcggta ggggctgtcg gaactctagg tgtagcggtg aaatgcgtag atatctagag
      181 gaacgccaat ggcgaaggca gacagctggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010464
VERSION     KHUT01010464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           93 g           46 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatcggg
       61 aaagtcttgt gtgaaagccc agagcttaac tctggaagtg cacgagatac tgctcgattt
      121 gagttcagga gaggaaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010465
VERSION     KHUT01010465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           90 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggtgtaa agggcgcgta ggcgggactg
       61 caagtcagat gtgaaatttt ccggcttaac cgggagcgtg catttgaaac tgtagttctt
      121 gagtgatgga gaggcaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttgctgga cattaactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010466
VERSION     KHUT01010466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           47 t
ORIGIN      
        1 tacggaggat gcgagcgttg ttcgaattga ttgggcgtaa agggcaggta ggtggtgctg
       61 cacgtccgaa gtgaaagcct tgggcttaac ccaagaagtg ctttggaaac tgcagaactg
      121 gagtcctgga gaggcgagtg gaattcccgg tgtagtggtg aaatacgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caggtactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010467
VERSION     KHUT01010467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggatgtt
       61 cgcgtcagag gtgaaagccc cgggctcaac ctgggaactg cctttgaaac gggacatctt
      121 gagttcggga gagggtagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctacctggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010468
VERSION     KHUT01010468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           63 c          106 g           32 t
ORIGIN      
        1 gacggagggt gctagcgttg tccggaatca ctgggcgtaa agggcgcgca ggcggccccg
       61 cccgtcgggc gtgaaagccc ggggctcaac cccggaacgg cgcccgagac ggcggggctg
      121 gagaccggga gaggagggtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccgggtctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010469
VERSION     KHUT01010469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          103 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agggcgtgta ggcggcctct
       61 taagtccggt gtgaaagccc ccggctcaac cggggagggt cactggaaac tggggggctt
      121 gagagcagga gaggagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctggctctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010470
VERSION     KHUT01010470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtcgta
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gatacgactg
      121 gagtaggtga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc accatactga cgctgaggcg cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01010471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010471
VERSION     KHUT01010471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctatg
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgatac tacgtagctt
      121 gagtcccgga gaggatggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010472
VERSION     KHUT01010472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 caagtcgggt gtgaaatccc cgggctcaac ctgggaactg cattcgaaac tgcactgctg
      121 gagtatggga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctaga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010473
VERSION     KHUT01010473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c          100 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcgggccgc
       61 caagtccggg gtgaaagctc ccggcccaac cgggaacgtg cctgggatac tggcggcctc
      121 gagggggata ggggcagacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gtctgctgga cccctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010474
VERSION     KHUT01010474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           88 g           54 t
ORIGIN      
        1 tacagggggt gcaagcgtta ttcggaatta ttgggcgtaa agagcgtgta ggcggttcag
       61 caagtttggt gtgaaatccc cgggcttaat ccgggaagtg cattgaatac tgctagacta
      121 gagtatagga gaggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg gccatctgga ctattactga cgctaaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010475
VERSION     KHUT01010475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          104 g           34 t
ORIGIN      
        1 tacggagggc gcgagcgtta cccggattca ctgggcgtaa agggcgtgta ggcggcctgg
       61 ggcgtcccat gtgaaagacc acggctcaac cgtgggggag cgtgggatac gctcaggcta
      121 gacggtggga gagggtggtg gaattcccgg agtagcggtg aaatgcgcag ataccgggag
      181 gaacgccgat ggcgaaggca gccacctggt ccacccgtga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010476
VERSION     KHUT01010476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           81 g           50 t
ORIGIN      
        1 tacagagacc tcaagcatta tccggattta ttgggcgtaa agggtccgca ggtggtcgtg
       61 cacgtcgggg gttaaatgta cgagctcaac tcgtacaccg cttccgatac gacatgacta
      121 gagggtggga gaggtgagcg gaattgccgg tgtagtagta atatgcgtta atatcggcaa
      181 gaacaccaaa tgcgaaggca gctcactgga acacttctga cactcaggga cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01010477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010477
VERSION     KHUT01010477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcccgc
       61 caagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgagac tggcgggctt
      121 gagggtcgga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga cgacttctga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010478
VERSION     KHUT01010478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           94 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggctggg
       61 tccgtccgat gtgaaagccc ccggctcaac cggggaattg catcggagac cgcccggctc
      121 gaggatggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccatctctga cgctaaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010479
VERSION     KHUT01010479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgat
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg cgtttgaaac tgatgggcta
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctggc ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010480
VERSION     KHUT01010480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           43 t
ORIGIN      
        1 tacagaggga gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgg
       61 tgtgtctacg gtgaaatccc tcggctcaac cgaggaagtg ccgtagaaac tgccgggctc
      121 gaggccggga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010481
VERSION     KHUT01010481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggttcga
       61 taagtctgct gtgaaagtcc aaggctcaac cttgggatgc cggtggatac tgtcgaactt
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gcactctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010482
VERSION     KHUT01010482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           49 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggctttg
       61 tgtgttccat gtgaaagccc tcggctcaac cgaggaattg catggaaaac tgcagagctt
      121 gagtccggga gaggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggtactga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010483
VERSION     KHUT01010483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           83 a           39 c           76 g           55 t
ORIGIN      
        1 gacgtaggat gcaaacgtta tccggaatta ttgggcgtaa agagttcgta gacgaactgt
       61 taagtagaat gttaaaggtc agggcctaac cctgataagg tgttctaaac tggcagaata
      121 gagtatagca gaggcaagtg gaattctgtg tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gcttgctggg ctattactga cgttcaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010484
VERSION     KHUT01010484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggttgtc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tggatgactg
      121 gagtgccgga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga cggtgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010485
VERSION     KHUT01010485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           86 g           46 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgcccaaaac taccgtgctt
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacccgt ggcgaaagcg gctcactggg tcttctctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010486
VERSION     KHUT01010486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatga ttgggcgtaa agggtgcgca ggcggtttgc
       61 aaagacggat gtgaaagccc taggcttaac ctgggaatgg catacgtgac tggcaaacta
      121 gaatatggca gagggaggtg aaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg tcaatattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010487
VERSION     KHUT01010487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatta ttgggcgtaa agagcatgta ggtggccttg
       61 taagtcaatg attaaatacc cacgcttaac gtggggactg ttattgatac tgcatggcta
      121 gaggaaaaca ggggtaagcg gaactttagg tgtaggagtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttactggg ttttttctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010488
VERSION     KHUT01010488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           89 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggtcttt
       61 taagtctgat gtgaaatctt gcggcttaac cgtaagcggt cattggaaac tggaggactt
      121 gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg actctctggt ctgtaactga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010489
VERSION     KHUT01010489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgggtgtctt
      121 gggtcatcta ggggctgccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaac ggcgaaagca ggcagctggg gatgcaccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010490
VERSION     KHUT01010490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggttaca
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgaaac tgtgtgactg
      121 gagtctcgga gaggtgggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaataccggc ggcgaaggcg gcccactgga cgaagactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010491
VERSION     KHUT01010491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggtgcgta ggcggccttt
       61 taagtcagat gtgaaagacc acggcttaac cgtggggttg catttgaaac tggagggctt
      121 gagtatcgga gagggtagtg gaattcccag tgtagcggtg gaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actacctgga cgaaaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010492
VERSION     KHUT01010492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcccga
       61 taagtcgaac gtgaaagccc ccggctcaac tggggagggt cgttcgatac tgtcaggctt
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggc ctgctcttga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010493
VERSION     KHUT01010493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           89 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtttgt
       61 taagtcgagt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac taactggcta
      121 gaagacggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctgga ccgatcttga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010494
VERSION     KHUT01010494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           55 c          105 g           44 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggtggttgcc
       61 tacgtccggt gtgaaatctc ccggctcacc tgggaggggg cgctggagac tgggcgactg
      121 gagacaggga gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gccggctgga cctgttctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010495
VERSION     KHUT01010495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           87 g           57 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggtggtgatc
       61 taagtctgtc gtgaaatctc ccggcttaac tgggagggtg cggcggaaac tggattgcta
      121 gagtatggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gcttactgga ccattactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010496
VERSION     KHUT01010496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttaga
       61 taagtaagtt gtgaaagccc cgggcttaac ctgggaatgg caacttaaac tgtttagcta
      121 gagtacatga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actacctggc atgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010497
VERSION     KHUT01010497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgctggta ggtggcttgg
       61 aaagtcgcgt gtgaaagctt ccggctcaac cggaaaagtg cgcgcgaaac tgacgagctt
      121 gagtgccgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga aggtaactga cactgagcag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010498
VERSION     KHUT01010498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggtttga
       61 taagtttgat gtgaaagccc agggctcaac cttggaactg cattgaatac tatcagacta
      121 gagtggtgga gaggaaagca gaattcccag tgtagaggtg aaattcgtag agattgggag
      181 gaataccaat ggcgcaggca gctttctgga cacacactga cgctgaggca cgatagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010499
VERSION     KHUT01010499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcggacccg
       61 caagtgcttt gtgaaatccc atggcttaac catggaatgg cttggcatac tgcgggtctt
      121 gaggtcggta gaggctgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgat ggtgaagaca ggcagctggg ccgaatctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010500             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010500
VERSION     KHUT01010500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           84 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgtgta ggtggaagtt
       61 cgcgtgcaat gcgaaatcgt gtggctcaac catatcgacg gtattgcata cgggacatct
      121 agaggacggc agaggcaagt ggaattggta gtgtagcagt gaaatgcgta gatattacca
      181 ggaacaccaa tggcgaaggc agcttgctgg gccgtttctg acactcagac acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010501
VERSION     KHUT01010501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           45 c           77 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggctgattaa
       61 caagttggat gtgaaatcct agggctcaac cttagaactg catccgaaac tgtcaatcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01010502             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010502
VERSION     KHUT01010502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           90 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 aaagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac ttccatgact
      121 tgagttcggt aggggacctc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agaggtctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010503             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010503
VERSION     KHUT01010503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           87 g           49 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agagtctgta ggctgcgact
       61 tgcgtgagat gtgaaatcct atggctcaac catatggact gtatctcata cggggtcgct
      121 agaggacggc agaggcaagt ggaactggcg gtgtaggggt gaaatccgta gatatcgcca
      181 agaacaccaa tggcgaaggc agcttgctgg gccgtccctg acgctgagag acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010504
VERSION     KHUT01010504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggtggtctga
       61 taagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac tgtcagactt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010505             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010505
VERSION     KHUT01010505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcggctcgg
       61 cacgtcggac gctgaaatcc cccggctcaa ccggggaagt ggcgccgata cggccgggct
      121 ggaggggggt aggggggcct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg acccttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010506             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010506
VERSION     KHUT01010506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagcgcgta ggcggcattg
       61 taagttggag gtgaaatccc atcggctcaa ccgtggaata gcccccaata ctgcagtgct
      121 cgagggaggt aggggcatgc ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc ggcatgctgg aactcttctg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01010507             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010507
VERSION     KHUT01010507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           80 g           63 t
ORIGIN      
        1 tacgggggcc cctagcatta tccggtttta ttgggcgtaa agagttctgt aggtggtgtt
       61 ataagttacg ggttaaatcc tccggcttaa ctggagatgt gctcgtaata ctgtgacact
      121 agaggcaaac aggggaaagt agaacgcatg gtgtagtggt aaaatacgtt gatatcatgc
      181 ggaataccga aggcgtaggc agctttctgg gtttgtcctg acactgagag acgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01010508             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010508
VERSION     KHUT01010508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           56 c           80 g           50 t
ORIGIN      
        1 tcccagcggt ccaagtcgca gccacgttta ttgggtctaa aacatccgta gcttgctttg
       61 taagtctcct gtgaaatctg gcctctcaag ggtcgggcgc gcaagagata ctgcaaagct
      121 ggggaccggg agacgtaagg agtacagaca gggtagcggt aaaatgcgtt gatcctatct
      181 ggactaacga tagcgaaggc accttacgag aacggatccg acagtgaggg atgaaggcta
      241 ggggcgcaaa atgg
//
LOCUS       KHUT01010509             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010509
VERSION     KHUT01010509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           44 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgag
       61 taagtcgggc gtgaaatttc agggctcaac cctgaaacgc cgctcgatac tgctctggct
      121 agagtccggt aggggagcgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccgg cggcgaaggc ggcgctctgg gccgagactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010510             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010510
VERSION     KHUT01010510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           51 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggcctgt
       61 taagtgtgaa gtgaaatttc cagcgctcaa cgcggaaact gcttcgcata ctggcaggct
      121 tgagggatgc agaggtaagt ggaattcctg gtgtagcggt gacatgcatt gatatcagga
      181 ggaacaccca tggcgaaggc agcttactgg gcatcatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010511
VERSION     KHUT01010511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcccgca ggcggtccgt
       61 taagtttcag gtgaaatctc ccggctcaac cgggagggtg cctggaaaac tggcggactt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010512
VERSION     KHUT01010512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           83 g           54 t
ORIGIN      
        1 tacgaaggga gcaagcgtta ctcggaatta ctgggcgtaa agcgcatgta ggcggaattg
       61 caagtcagag gtgaaatccc agggcttaac cctggaattg cctttgaaac tgcagttctt
      121 gagtatgaca gaggatggtg gaatttccag tgtagaggtg aaattcgtag atattggaag
      181 gaacaccggt ggcgaaggcg accatctggg tcattactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010513             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010513
VERSION     KHUT01010513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           83 g           50 t
ORIGIN      
        1 aacgagtgcc ccaagcgtta tccggaatta ctgggcataa agggtgcgta ggtggtctga
       61 ttagtcgttt gttaaaactc ccggcctaac cgggaaggtg cagacgaaac ggtcagacta
      121 gagagagcga gaggttagca gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaataccgaa ggcgaaggca gctaactggc gcttattctg acactccagc acgaaagcgt
      241 gggtcgcgaa cggg
//
LOCUS       KHUT01010514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010514
VERSION     KHUT01010514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtttc
       61 caagtccggg gtgaaatcct ccagctcaac tggagaagtg ccttggatac tggaagcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010515
VERSION     KHUT01010515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccgc
       61 taagtcggga gtgaaagccc tcggctcaac cgaggaagtg ctttcgaaac tgacggactt
      121 gaatactgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010516
VERSION     KHUT01010516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaatg gtgaaatcct ccggcttaac cggagaagtg ccaatgatac tggcgagctc
      121 gagggaggta ggggcagctg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg agctgctgga tctctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010517
VERSION     KHUT01010517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcc
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaagtg cctcggatac tgggagcctt
      121 gagggaggca ggggcggatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010518
VERSION     KHUT01010518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatccg
       61 caagtcgggc gtgaaatccc tgggctcaac ccgggaactg cactcgaaac tgcgggtctt
      121 gagtaacgga gaggatcgcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgatctgga cgtttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010519
VERSION     KHUT01010519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           86 g           55 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggattta ttgggcgtaa agcgtacgca ggcggttgat
       61 taagtctgac gtgaaacctc ccggcttaac tgggagcagt cgttggaaac taattgactt
      121 gaggcaatga gaggtaggca gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcctaccagc attgtactga cgctcatgta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010520
VERSION     KHUT01010520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcagag gtgaaatgcc caggctcaac ctgggaactg cctttgaaac tgtagagcta
      121 gagtattgga ggggaatggg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg ccattctgga caattactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010521
VERSION     KHUT01010521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggagcgta ggcgggttat
       61 caagttgggg gtgaaagccc ggggctcaac ctcggaattg ccttcaaaac tgataacctg
      121 gagtatggaa gaggtaagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acttactggt ccattactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010522
VERSION     KHUT01010522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgtg
       61 taagttaggt gtgaaagtcc tgggcttaac ctggggagga cgcttaaaac tgcaaagcta
      121 gagtactgta gagggaagtg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga cagatattga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010523
VERSION     KHUT01010523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           92 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgcgta ggcggggatg
       61 caagtcagat gtgaaatacc gaggcttaac ttcggggctg catctgaaac tgtatttctt
      121 gagtgtcgga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg actttctgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010524
VERSION     KHUT01010524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c          103 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtgcgg
       61 taagttgggc gtgaaatccc cgggctcaac ccgggggggc cgtccaggac tgccgtgcta
      121 gggtgtggga gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggcgaaagcg gctacctgga ccataaccga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010525
VERSION     KHUT01010525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           92 a           28 c           65 g           68 t
ORIGIN      
        1 tacgaaagga gcaggtgtta ttcagattaa ctgggcgtaa agggcacgta ggtggtttgt
       61 tatgtgtact ataaaattat aaagcataac tttgtaatgt agtataaaca actaaactta
      121 gagttaggta gagggtagta gaatttctaa tgtaaaggtg aaatttgata aaattagaga
      181 gaataccaat agcgtaggca actacctaga ccttaactga cactgaggtg cgaaggtatg
      241 gggagcaaat agg
//
LOCUS       KHUT01010526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010526
VERSION     KHUT01010526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          104 g           43 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggtggcagcc
       61 caagtctgat gtgaaagcgc ccggctcaac tgggcgaggt cattggaaac tgggcagctg
      121 gaggacggta gaggcaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gcctgctggg ccggtcctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010527
VERSION     KHUT01010527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           87 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggaaga
       61 aaagtcagat gtgaaatccc tgggctcaac ccaggaactg catttgaaac tctctttctt
      121 gagtatcgga gaggaaggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gccttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010528
VERSION     KHUT01010528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggcttc
       61 taagttaggt gtttaatcct ggggcccaac cccagtcgtg cacttaaaac tggaagtctt
      121 gaatagtgga gaggtcagtg gaattggggg tgtagcggtg gaatgcgtag agatccccaa
      181 gaacaccagt tgcgaaggcg gctgactgga cacatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010529
VERSION     KHUT01010529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           81 g           59 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggcggtttca
       61 taagttagat gtgaaagccc agggcttaac cctggaattg catttaagac tgtggaactt
      121 gaagttcgaa gaggttagca gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaataccaat ggcgtaggca gctaactggt cgacacttga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010530
VERSION     KHUT01010530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           92 g           41 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacctc
       61 taagtacctt gtgaaatccc acggctcacc cgtggaaccg ctgggtatac tgggggtctt
      121 gggccactca ggggcgactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggtcgctggg agtgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010531
VERSION     KHUT01010531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggccaag
       61 cacgtcagat gtgaaatccc tgggcttaac ctaggaattg catttgatac ggcttggcta
      121 gagttcagca gagggtggtg gaattgcgag tgtagaggtg aaattcgtag atattcgcag
      181 gaacaccagt ggcgaaggcg gccacctggg ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010532
VERSION     KHUT01010532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           97 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtcggct gtgaaagtcg agggctcaac cctcgaatgc cggccgaaac tgccgggcta
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010533
VERSION     KHUT01010533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          101 g           42 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcgccg
       61 gaagtcgggc gtgaaagccc cgggctcaac ctgggaattg cgcttgatac tccggtgctg
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010534
VERSION     KHUT01010534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           88 g           46 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctccg
       61 caagtcggat gtgaaagcct tcggcttaac cgaagaagtg catccgaaac tgcagagctt
      121 gagtacttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010535
VERSION     KHUT01010535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttatt
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catacaatac tgagtagcta
      121 gagtgtggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010536
VERSION     KHUT01010536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           97 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggacggt
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaacgtg cctccgatac tggccgtctc
      121 gagggaggca ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010537
VERSION     KHUT01010537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           80 g           54 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggattta ttgggcgtaa agggtgcgca ggcggtatat
       61 taagtcaact gttaaatacc tcagcctaac tgaggaccag cggtagatac tggtatacta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccacatctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010538
VERSION     KHUT01010538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           94 g           42 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggccctg
       61 taagtccgct gtgaaagtcc aaggctcaac cttggaatgc cggtggatac tgcagggcta
      121 gagtccggaa gaggcaagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaac ggcgaaggca gcttgctggg acggtactga cgctaaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010539
VERSION     KHUT01010539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacggggggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggcggtttgt
       61 taagtctgat gtgaaagccc cgggcctaac ccgggaagtg cattggatac tggcagactt
      121 gaattcggga gagggcagcg gaattcctgg agtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccgcctgga ccgacattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010540
VERSION     KHUT01010540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 taagttgggt gtgaaatacc tcggcttaac cgaggaacag cgctcaaaac tactgtgctt
      121 gagggagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010541
VERSION     KHUT01010541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           85 g           52 t
ORIGIN      
        1 tacgaagggt gtaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggtggatttt
       61 taagtcagat gtgaaatctc ggggctcaac cccgaacgtg cgtctgaaac tggagatcta
      121 gaatattgca gggggtagag gaatttcaca tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccaga ggcgaaggcg tctacctggg caaacattga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010542
VERSION     KHUT01010542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcacgta ggcggtagaa
       61 tgtgtgtgat gtgaaagccc cgggctcaac ctgggaagtg catcgcaaac tatttaacta
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010543
VERSION     KHUT01010543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtccgtt gtgaaaactc gaggctcaac ctcgagacgt cggcggaaac cctctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010544
VERSION     KHUT01010544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctga
       61 caagtctggg gtgaaatccc acggctcaac cgtggaactg ccttagatac tgtcggcctc
      121 gaggaaggta ggggcatgcg gaactaacgg tggagcggtg aaatgcgttg atatcgttag
      181 gaactccggt ggcgaaagcg gcgtgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010545
VERSION     KHUT01010545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           84 g           54 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtttgta ggcggctcta
       61 taagtcgggt gtgaaatccc acggctcaac cgtggaagtg cattcgaaac tgcagagctt
      121 gaatatggta gaggagagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg actctctgga ccaatattga cgctgagaaa cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010546
VERSION     KHUT01010546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcgggcttt
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaactg cctttgatac tgggaatcta
      121 gagtatggta ggggcgtgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcacgctgga ccattactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010547
VERSION     KHUT01010547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           84 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtttgat gtgaaatctc cgggcttaac ccggaaactg cattgaatac tatcccactc
      121 gaggtttgga agggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010548
VERSION     KHUT01010548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           85 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggttcgc
       61 caagtttgga gtgacagttg tcggctcaac cgacaaagga cttcaaaaac tggtgaactt
      121 gaggacgaga gaggaacatg gaactccagg tggagtagtg aaatgcgtag atatctggaa
      181 gaacaccaat ggcgaaggca atgttctggc tcgtttctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010549
VERSION     KHUT01010549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggctttg
       61 caagccagag gtgaaagcct ggagctcaac tccagaactg cctttggaac tgcatagcta
      121 gagtgtcgga ggggatagcg gaatttctaa tgtagcggtg aaatgcgtag atattagaag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010550
VERSION     KHUT01010550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c           97 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggggac
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaattg ccttggatac tggttctctc
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ggctgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010551
VERSION     KHUT01010551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcgt
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaacag ccctggatac tgacggtctc
      121 gagtgaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010552
VERSION     KHUT01010552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggtcaaa
       61 caagtcggat gtgaaagccc tcggcttaac cgaggaagtg cgtccgaaac tatttggctt
      121 gagtacggga gaggaggatg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacctgt ggcgaaggcg attctctaga ccgtaactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010553
VERSION     KHUT01010553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           49 c          100 g           52 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcatcc gtgaaatttt ggggctcaac cctgagattg cggttgatac gggcgggctt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010554
VERSION     KHUT01010554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           89 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggtgcgta ggtggttgat
       61 taagtgagat gtgaaagccc cgggcttaac ctgggaattg catcttatac tggtcagctt
      121 gagtgaagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cttatactga cactgaggca cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010555
VERSION     KHUT01010555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           77 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggactta
       61 taagtcagtg gtgaaagccc ggagctcaac tccggaactg ccattgatac tgtaggtctt
      121 gagtacactt gaggtgggcg gaatacatca tgtagtggtg aaatacttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaaa ttgtaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010556
VERSION     KHUT01010556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 tacgaaggtg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggatttg
       61 taagtcgatt gtgaaatccc caggctcaac ctgggaattg cagttgatac tgcagatctt
      121 gaatgctcga gaggatgata gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaataccgga ggcgaaagcg gtcatctggc gagacattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010557
VERSION     KHUT01010557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggttgtcttg
       61 taagtctact gtgaaatccc tgggcttaac ccaggaagtg cggtggatac tgcaggacta
      121 gagtgccgga ggggtacgtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccaga ggcgaaggcg gcgtactgga cggcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010558
VERSION     KHUT01010558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccctg
       61 caagtccttc gtgaaatcct gcggctcaac cgcagacgtg cgcgggatac tgcagggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg acggcactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010559
VERSION     KHUT01010559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           84 g           50 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggtcgtg
       61 ttagtctcat gttaaagctc ccggcttaac cgggaaatcg catgggatac ggcacaacta
      121 gaggatgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca attcactggc gcactcctga cgctgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010560
VERSION     KHUT01010560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           87 g           50 t
ORIGIN      
        1 tacgtaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtacgta ggcggctctg
       61 tcagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac tgcagagctg
      121 gagtgagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaggcg gcttactggg tctctactga cgctgaggta cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01010561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010561
VERSION     KHUT01010561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           86 g           53 t
ORIGIN      
        1 cacgaaccgt gcgaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggcgtgt
       61 taagttgggt gtgaaatccc ttggctcaac caaggaattg cgctcaaaac tagcatgctt
      121 gaggaagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgtag atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010562
VERSION     KHUT01010562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           46 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcagct
       61 taagtaaggt gtgaaatctc cctgctcaac ggggagggtg cactttagac tgggcagcta
      121 gaggtgggta gagggtggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccacctggg ccttacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010563
VERSION     KHUT01010563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggcgtta
       61 ttagtctttt gtcaaagctc cgagctcaac ttggaaaagg cagaagaaac ggtaatgcta
      121 gagagtgtga gaggtttgcg gaactaatgg tgtaggggtg aaatccgttg atatcattgg
      181 gaacaccgaa ggcgaaggca gcaaactggc acatttctga cgctcaagca cgaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01010564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010564
VERSION     KHUT01010564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           74 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtcat
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tattgatctt
      121 gaattttgtt gaggtaggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gcttactaag caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010565
VERSION     KHUT01010565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggct gtgaaaacct ggggctcaac cccaggcctg cagtcgatac gggcagacta
      121 gagtccggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccggaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010566
VERSION     KHUT01010566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           85 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggaatga
       61 taagtcagag gtgaaatctc acagcttaac tgtgaaactg cctttgatac tgtcattctt
      121 gagttcggga gaggctgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcagctggc ccgtaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010567
VERSION     KHUT01010567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           90 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctga
       61 taagtcggat gtgaaatccc tcggctcaac cgaggacgtg cattcgaaac tgtcaggctc
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010568
VERSION     KHUT01010568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           83 g           53 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta gacggcacat
       61 taagtttatt gttaaagcct tcggcttaac cggagaatcg caataaaaac tgatgagcta
      121 gaggttgaga gagaggagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggca gctccttggc tcaaatctga cgttgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010569
VERSION     KHUT01010569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           85 g           52 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggttcgta ggcggctttg
       61 taagtgagat gtgaaatccc acggctcaac cgtggaagtg catttcatac tgcaaggctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010570
VERSION     KHUT01010570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           90 g           46 t
ORIGIN      
        1 gacgtagggg ccaagcgtta ttcggaatca ttgggcgtaa agcgctcgta ggcggctcga
       61 aaagtccgtg aagaaagacc tgagctcaac tcagggaacg gcacggatac tcttgagctt
      121 gaggtaatca gagggtgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcacctggg gttattctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010571
VERSION     KHUT01010571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgtgtgta ggcggtcttg
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaagtg cactcgatac tgcaagactt
      121 gagtactgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgggaa
      181 gaacatcggt ggcgaaggcg gccaactgga cagatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010572
VERSION     KHUT01010572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtctgc
       61 taagtctggt ttgaaagctg gtggctcaac catcagatgt ggctggaaac tggcggactt
      121 gaatgggttg gccgtcgctg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggcgacgagg acctgattga cactgaggca cgagagcatg
      241 gggagccaac agg
//
LOCUS       KHUT01010573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010573
VERSION     KHUT01010573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcttag
       61 aaagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac ttcttagcta
      121 gagggtggtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gttttctggg ccaatcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010574
VERSION     KHUT01010574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           78 g           59 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgcaggta ggcgggcttg
       61 ttagtcgtat gttaaagctt ggggcttaac cccaaagatg catacgaaac ggcaagtctg
      121 gaggatgtga gagatcagtg gaatttatgg tgtagtagtg aaatgcgttg atatcataaa
      181 gaacacccaa ggcgaaggca actgattggc acatttctga cgctgagctg cgaaagcgtg
      241 ggtagcgaat agg
//
LOCUS       KHUT01010575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010575
VERSION     KHUT01010575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           84 g           51 t
ORIGIN      
        1 tacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctatt gtgaaagctc ccggcttaac tgggagaggt caatagaaac tatcaggctt
      121 gagtatggga gaggatgctg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaagca ggcatctgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010576
VERSION     KHUT01010576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           90 g           43 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggccttg
       61 caagtcggat gtgaaatccc acggctcaac cgtggacgtg catccgaaac tgctcggctc
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010577
VERSION     KHUT01010577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           91 g           55 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggttgttcgt
       61 taagtctggt gtaaaagact ggggcccaac cctgggatcg cactggatac tggcggacta
      121 gagtgaagta gaggcaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaagcg gcttgctggg ctttaactga cgctgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01010578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010578
VERSION     KHUT01010578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           88 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttacg
       61 taagttagat gtgaaagccc tgggcttaac ctgggaattg cgtttgatac tgcgtggcta
      121 gagtattgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga caaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010579
VERSION     KHUT01010579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          101 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcggag
       61 aaagtcaacg gtgaaagctc ggggctcaac cccgagtcgg ccgtcgatac ttctttgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010580
VERSION     KHUT01010580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggcccag
       61 taagtcagtg gtgaaatctc ccggctcaac cgggaaattg ccattgatac tgctgggctt
      121 gaattactgg gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactacc agtggattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010581
VERSION     KHUT01010581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           89 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggtctgt
       61 taagtctgag gtgaaatcta tcggctcaac cgggagaggc cctcagatac tggtaggctt
      121 gaggatagta gaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg atcttctgga ctattcctga cgctgaggcg caaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010582
VERSION     KHUT01010582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           80 g           57 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactgg
       61 ttagtctggg gtgaaatctc atcgcttaac ggtgaaactg ccttagatac tgccagtctt
      121 gaatcagcga gagatatgcg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcatattggc gctcgattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01010583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010583
VERSION     KHUT01010583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ttgggcgtaa agcgcgcgta ggcggccaga
       61 caagtccggt gtgaaagtca aaggctcaac ctttgaatgc cgctggaaac tgtctggcta
      121 gagtccggaa ggggcgagtg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010584
VERSION     KHUT01010584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggcggctctg
       61 taagtcagga gtgaaagccc cgagcttaac ttgggaagtg cttttgatac tgcagagctt
      121 gaatgtggga gaggttagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctaactggc ccaacattga cgctgagacc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01010585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010585
VERSION     KHUT01010585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           83 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagtgcgta ggtggttgga
       61 taagttaggt gtgaaatccc tgggctcaac ctaggaactg cgcttaaaac tgttcggcta
      121 gagtattgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctggg caaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010586
VERSION     KHUT01010586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           39 c           87 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggtttta
       61 taagtcagtg gtgaaagctc agagctcaac tctggatgtg ccattgatac tgtaggactt
      121 gagagcagtt gaggtagctg gaatgtgtag tgtagcggtg aaatgcatag agattacaca
      181 gaacaccgat tgcgaaggca ggttactagg ctgtttctga cgctgaggca cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010587
VERSION     KHUT01010587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcatta
       61 taagtcggat gtgaaagccc atggctcaac catggaagtg cattcgaaac tgtagagcta
      121 gagtcttgga gagggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accacctggc tgaagactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010588
VERSION     KHUT01010588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           96 g           48 t
ORIGIN      
        1 tacggaggtt gcgagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggtttgg
       61 taagtctgga gtgaaagcac ggggctcaac cccgtcgggt ctttggatac tgccaaactt
      121 gagtacgtga gaggagagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctctctggc acgaaactga cgctgaggct cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010589
VERSION     KHUT01010589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          101 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 cgcgtccggg gtgaaagcct ccagctcaac tggagaagtg cctcggatac ggccggcctc
      121 gagtggggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcacgctgga cccctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010590
VERSION     KHUT01010590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           92 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggccacc
       61 ccagtcgggt gtgaaagccc caggctcaac ctgggaagtg cacacgatac taggtggctg
      121 gagtctcgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cgagtactga cactgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010591
VERSION     KHUT01010591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggtctag
       61 ttagtcagat gtgaaatccc tgggcttaac ccaggaaccg catctgatac tgctagactt
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agacaactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010592
VERSION     KHUT01010592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggttggta ggtggtctta
       61 tttgtctgag gtgaaatccc ggggcttaac cccggaagtg ccttagaaac ggtaggactt
      121 gagtcctaga ggggtttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga agcgaaggcg gcaaactgga taggaactga cactgaacaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010593
VERSION     KHUT01010593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagcgta ggttgctcga
       61 tgtgtcagtg gtgaaatccc ggagcttaac ttcggaactg ccattgaaac tgtcgagcta
      121 gaatctcaga gggggtagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctacctgga tgagtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010594
VERSION     KHUT01010594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcctt
       61 taagtccgtg gtgaaatacc agtgcttaac actggaactg ccatggatac tattggtctt
      121 gaatgttgat gaggtgggcg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gctcactaaa caacgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010595
VERSION     KHUT01010595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcatgta ggcggtctgc
       61 taagtcgcgt gtgaaagctt ccagctcaac tggaaaagtg cgcgcgaaac tggtggactt
      121 gagtgctgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cagacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010596
VERSION     KHUT01010596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           95 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcagag gtgaaagccc acggctcaac cgtggaagtg cccctgatac tgatcgtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010597
VERSION     KHUT01010597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           82 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggcctgt
       61 taagtcaggg gtgaaagacg gtagcttaac tatcgcagtg cccttgatac tgacgggctt
      121 gaatggacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactatg gtccgattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010598
VERSION     KHUT01010598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcgcgca ggtggcctgt
       61 taagttgggg gtgaaagccc ggagctcaac tccggaattg ccttcaagac tggcaggcta
      121 gagtacggca gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ccgttactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010599
VERSION     KHUT01010599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggattta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtcttct gtgaaagctc ccggcttaac tgggagaggt caggggaaac tgtcaggctt
      121 gagtatggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010600
VERSION     KHUT01010600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggattta ttgggcgtaa agcgcgtgta ggcggctcgt
       61 taagtcggct gtgaaagccc atggctcaac catggaagtg cagccgaaac tggcaagctt
      121 gagtcccgaa gagggtggcg gaatgcccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccagt ggcgaaggcg gccacctggg agacgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010601
VERSION     KHUT01010601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta gacggttatg
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgagac tggatagcta
      121 gggtacggaa gagggaagcg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaagcg gcttcctggt ccagtaccga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010602
VERSION     KHUT01010602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggtggcgggt
       61 taagtcgaat gttaaagcct gaggctcaac ctcagagatg cactcgatac tggcctgcta
      121 gagggtgata gaggaaagcg gaattcccaa tgtagcggtg aaatgcgtag atattgggag
      181 gaacatcagt agcgaaggcg gctttctggg tcacacctga cactgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010603
VERSION     KHUT01010603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           96 g           51 t
ORIGIN      
        1 tacgtgaggg gcgaacgttg ttcggtgtca ctgggcttaa agggcgcgta ggtggttgcg
       61 taagtaacgt gtgaaatccc tcggcttacc cggggaattg cacgttatac tgcgtgactt
      121 gaggatcaga ggggaatggg gaactccagg tgtagcggtg aaatgcgcag atatctgggg
      181 gaaggccggc ggcgaaggcg ccattctggc tgattcctga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010604
VERSION     KHUT01010604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcttgg
       61 caagttgggt gtgaaatacc tcggctcaac cgaggaatag cgctcaaaac tgctgagctt
      121 gagggagata gaggtgagcg gaacttaagg tggagcggtg aaatgcgttg atatcttaag
      181 gaacacccgt ggcgaaagcg gctcactgga tctcttctga cgctcaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010605
VERSION     KHUT01010605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          101 g           39 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcatgta ggcggctggg
       61 aaagtccgtg gttaaagccc ctggctcaac tgggggaggt ccacggagac tactcgactt
      121 gagggcagca gaggcgcgtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gcgcgctggg ctgcacctga cgctgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010606
VERSION     KHUT01010606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtcatg
       61 caagtgagat gtgaaatccc cgggcttaac ctgggaactg cattttagac tgtgtgactg
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010607
VERSION     KHUT01010607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtatct
       61 caagtcaggg gtgaaagccc ggagctcaac tccggaattg cctttgaaac taggacactt
      121 gaacgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgttga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010608
VERSION     KHUT01010608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ttgggcgtaa agcgcacgca ggcggttctc
       61 catgttcaaa gtgacagtcg tcggctcaac tgacgaagtg ctaggaagac gggagaactt
      121 gagggctcac gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca gtgtcctggg gagtcgctga cgctcaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010609
VERSION     KHUT01010609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           84 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggtggttaga
       61 taagttagat gtgaaaaccc tgggcttaac ctgggaactg cgtttaagac tgtttagcta
      121 gagtattgta gagggtagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actacctgga cagatactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010610
VERSION     KHUT01010610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggcttgg
       61 taagtcgaat gtgaaagccc tgggctcaac tcaggacgtg cattcgatac tgcccggctt
      121 gaatcctgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg accacctgga cagagattga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010611
VERSION     KHUT01010611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           84 g           51 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agggttcgta ggcggaccgg
       61 aaagtcagat gtgaaatccc acagctcaac tgtggaactg catttgaaac ttccggacta
      121 gagtgtgaga ggggtaaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg ctttactggc tcactactga cgctcaggaa cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010612
VERSION     KHUT01010612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           49 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcatgta ggcggttgga
       61 taagtctgtt gttaaagccc ctggctcaac tgggggaggt caatggagac tgtccgactt
      121 gagggcaaca gaggcacatg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gtgtgctggg ttgcgactga cgctgagatg cgaaagtgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010613
VERSION     KHUT01010613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           88 g           56 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgagtgta ggtggcttgt
       61 taagtcagat gtgaaatccc tgggctcaac cgaggaagtg catttgaaac tagcgagctg
      121 gagagtggta gaggaaggtg gaattccgtg tgtagaggtg aaattcgtag atacacggag
      181 gaataccggt ggcgaaggcg gccttctgga ccatttctga cactaagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010614
VERSION     KHUT01010614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtatgca ggcgggtcga
       61 taagtcagtg gtgaaatccc gaggctcaac ctcggaactg cctctgatac tgtcgatctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010615
VERSION     KHUT01010615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggttggg
       61 taagtctgat gtgaaatccc ggggctcaac cctggaactg cattggatac tgcctgacta
      121 gagaactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010616
VERSION     KHUT01010616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           84 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgtgtcta ggtgggtgga
       61 taagttagat gttaaagatc caagctcaac ttggggattg cgtttgatac tgtccatctt
      121 gagttaggga gggatgagtg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacacctgt ggcgaaagcg actcattggc cctatactga cgctcaagca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010617
VERSION     KHUT01010617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgca gacggcatta
       61 caagtctgat gtgaaagccc ggggctcaac cccggaactg cattggaaac tgtagagctc
      121 gagtgtcgga ggggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gcttactgga cgactactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010618
VERSION     KHUT01010618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 taagtctgat gtgaaaggtt ggggctcaac cctgacactg cattggatac tgccttgcta
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010619
VERSION     KHUT01010619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           85 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgtgtgta ggtggcttgc
       61 taagttgatt gtgaaatccc tgggctcaac cgaggaagtg cagtcaaaac tagcaggcta
      121 gaagatggca gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggc ggcgaaggcg gccttctgga ccattcttga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010620
VERSION     KHUT01010620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           86 g           48 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatca ctgggcgtaa agcgcgcgta ggtggatgtt
       61 taagtcgaat gtgaaagccc cgggcttaac ctgggaagtg catccgatac tgaatgtcta
      121 gagtatggta gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcctcctgga ccaatactga cactcaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010621
VERSION     KHUT01010621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 caagtctggt gtgaaatctt tcggcttaac cggaagcggt cactggatac tgccgggctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010622
VERSION     KHUT01010622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggctgtgcga
       61 caagtcagtg gtgaaatccc gaggctcaac ctcggaactg ccattgaaac tgtcgtgcta
      121 gaatctcaga gggggtagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctacctgga tgagtattga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010623
VERSION     KHUT01010623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           81 g           52 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agcgtgcgca ggtggtcgtg
       61 aaagttagaa gtgaaagccc agggcttaac cttggaattg cttttaaaac tccacgacta
      121 gagtactgga ggggttggcg gaatttcgag tgtagcagtg aaatgcgtag atattcgaag
      181 gaacaccgat ggcgtaggca gccaactaga cagtaactga cactcatgca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010624
VERSION     KHUT01010624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           95 g           48 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggcttgg
       61 taagtcgaat gtgaaagccc gtggcttaac catggaagtg cattcgaaac tactaggcta
      121 gagtgtcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg accgcctgga cgactactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010625
VERSION     KHUT01010625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttat
       61 taagtccgtg gtgaaagacg ctggctcaac cagcgaactg ccatggatac tgttagactt
      121 gagtgtggac gagggcggcg gaatagatgg tgtagcggtg aaatgcatag ataccatcta
      181 gaaccccgat tgcgtaggca gctgcctagg ccacaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010626
VERSION     KHUT01010626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctttgatac tggccgtctg
      121 gagggaggta ggggcgtgcg gaactttggg tggagcggtg aaatgcgtag atatccaaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010627
VERSION     KHUT01010627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 tacgaggggg gcaagtgttg ttcggaatta ctgggcgtaa agggagcgta ggcgggctct
       61 taagttggat gtttaaaacc ggggcctaac cccggcgtgg catccaaaac tgggagtctt
      121 gaatgggaca gaggccagtg gaattagagg tgtagcggtg gaatgcgtag atatctctaa
      181 gaacaccagt tgcgaaggcg gctggctggg tccacattga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010628
VERSION     KHUT01010628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgta ggcggtgcgt
       61 aaagtctgtc gttaaagccc ctggcttaac tagggatgtg cggcggatac taacgtgcta
      121 gaggatggaa gagaaaagcg gaactctcgg agtagcggta aaatgcttag atcccgagag
      181 gaacaccaat ggcgaaggca gctttttggt ccattcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010629             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010629
VERSION     KHUT01010629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           89 g           57 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 aaagtcggat gtgaaatctc tgggcttaac tcagagaggt catccgatac ttccatgact
      121 tgagtctggt aggggagtgt ggaatttctg gtgtagcggt gaaatgcgca gatatcagaa
      181 ggaacacccg tggcgaaggc ggcactctgg gccagtactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01010630             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010630
VERSION     KHUT01010630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           83 g           50 t
ORIGIN      
        1 tacgagagcc tcaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggtaatg
       61 ttagtcctgt gtcaaagcct gtcgcttaac gacagatccg catgggaaac ggcataacta
      121 agagggtgcg agaggtgtac agaactcacg gtgtaggggt gaaatccgtt gatatcgtgg
      181 ggaatgccaa aagcgaaggc agtacactgg cgcatacctg acgctgaaac acgaaagcca
      241 gggtagcgaa tggg
//
LOCUS       KHUT01010631             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010631
VERSION     KHUT01010631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          101 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggctgga
       61 taagtccagg ggtgaaatgc cggagctcaa ctccgtgcgt gccccggata ctgtctggct
      121 ggagacacgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gagatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010632             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010632
VERSION     KHUT01010632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c          103 g           39 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agagcgcgta ggtggcgcga
       61 tacgaccggt gtgaaatcct ccggctcaac tggagggggt cactggtaac ggtcgtgcta
      121 agagtgcagg agagggacgt ggaactccgg gagtagcggt gaaatgcgta gagacccgga
      181 ggaacaccag tggcgaaggc ggcgtcctgg cctgcgactg acgctgtggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010633
VERSION     KHUT01010633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           89 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttatct tagaaagacc tgggcttaac ctgggtatat gagataagac tgttaggctt
      121 gagtatagca gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggg ctaatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010634
VERSION     KHUT01010634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          107 g           33 t
ORIGIN      
        1 tacagagggc gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgacg
       61 caagcgggcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgcgtcgctc
      121 gagcatggta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ccatggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010635             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010635
VERSION     KHUT01010635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcgcgta ggcggattgc
       61 cgcgtcggaa tctgaaatcc cccggctcaa ccggggaact gggtccgaaa cgggcagtct
      121 tgagggacgt agggggggct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagcccctgg acgtctgctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010636             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010636
VERSION     KHUT01010636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           56 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgtgta ggcggaagtt
       61 taagtgaaat gcgaaatctc gtggctcaac catgtagact gtatttcata ctgggcttct
      121 agaggatggc agaggcaagt ggaattggta gtgtagcagt gaaatgcgta gatattacca
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatttctg acgctgagac acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010637
VERSION     KHUT01010637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcgtaa agagcgcgta ggcggtttag
       61 caagtctaat gtgaaatccc tgaactcaat tcaggaagtg catcggatac tgctaggctt
      121 gagtacagga gaggaaggcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gccttctgga ctgtcactga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010638
VERSION     KHUT01010638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           95 g           42 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ttgggcgtaa agcgtgcgta ggcggtccgc
       61 aaggtccgag gtcaaagcgt ggggctcaac cccataggtg cctcggaaac cggcgggcta
      121 gaggacggca gaggaaggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gccttctggg ccatacctga cgctgaggca cgaaagctcg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010639
VERSION     KHUT01010639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtgtg
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tatatatctt
      121 gaataccatg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg cggttattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010640
VERSION     KHUT01010640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggcatga
       61 aaagcagggt gtgaaagccc tcggctcaac cgaggaattg cgctctgaac tgtcgtgcta
      121 gaggaatcta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg gattttctga cgctgaggaa cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010641
VERSION     KHUT01010641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ttgggcgtaa agggagcgta ggcggttcgg
       61 caagtcttta gtgaaatctc tgggctcaac tcagagtctg ctggagaaac tgccgggctg
      121 gagtatggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccattactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010642
VERSION     KHUT01010642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 gacgtaggat gcgagcgttg ttcggattta ttgggcgtaa agcgtacgta ggcggttcgg
       61 taagtgggag gttaaagact agggctcaac cctaggagtg ccccccaaac tgtcggacta
      121 gaggatcata ggggaaggtg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacgccagt ggcgaaagcg accttctggg tgacacctga cgctgaggta cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010643             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010643
VERSION     KHUT01010643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          101 g           44 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggactgt
       61 taagtctggc gtgaaatctc caccgctcac cggggagagg tcgctggaaa ctggcagtct
      121 ggagttcggc agagggtcat ggaattccgg gtgtagcggt ggaatgcgta gatatccgga
      181 agaacaccgg tggcgaaggc ggtgacctgg gccgaaactg acgctgagga gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010644
VERSION     KHUT01010644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggaggct
       61 taagcgtgcc ttgaaagccc ccggctcaac cggggaaggt gggtgcgaac tgggtctctt
      121 gagggcgata gaggggggcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccccctggg tcggccctga cactgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010645             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010645
VERSION     KHUT01010645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           98 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtttct
       61 taagtttggg gtgaaaggtt caacgctcaa cgtgaacagt gcctcggata ctgggagact
      121 tgagttcggg agagggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc gactgcctgg cctagaactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010646             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010646
VERSION     KHUT01010646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           51 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcga
       61 taagtcgggt gtgaaaactc tgggcttaac ccagagacgc cacctgatac tgtcgtgact
      121 ggagtctggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tggcgaaggc ggcactctgg gccagtactg acactgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01010647             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010647
VERSION     KHUT01010647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           56 c           85 g           46 t
ORIGIN      
        1 caccagcgcc acgagtggtt atcacgatta ttgggcctaa agcgttcgta gccggtttgg
       61 taaatctctt gtgaaattgt tcggcttaac cggacagcgt gcaggagaga ctgccagact
      121 cgagaccggg aggggtcaga agtattccgt ggggaccggt aaaatgggat aatccacgga
      181 ggactaccga tggcgaaggc atctgaccag aacggatctg acggtgagga acgaaagcca
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01010648             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010648
VERSION     KHUT01010648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           83 g           56 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agagtgcgta ggcgggtttt
       61 taagtgtaat gcgaaatcct gtggctcaac cacatggact gtattgcata ctggatacct
      121 tgaggaatgc agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gagatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg gcattttctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010649             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010649
VERSION     KHUT01010649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           91 g           42 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatca ttgggcgtaa agcgtccgta ggtggcgaag
       61 caagtctgct gtcaaagccc acagcttaac tgtggaaagg cagtggaaac tgcagcgcta
      121 gagtgcggta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag agattgggaa
      181 gaacaccggt ggcgaaagcg ctctactggg ccgcaactga cactcacagg acgaaagcta
      241 ggggagcgaa tggg
//
LOCUS       KHUT01010650             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010650
VERSION     KHUT01010650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           49 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtatgaa gtgaaatctc cagggctcaa cccggaaacc gctttgtaaa ctggcagact
      121 ggaggaaggc agaggtgagt ggaattcccg gtgtagcggt gacatgcatt gatatcggga
      181 ggaacaccca tggcgaaggc agctcactgg gcctttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa caga
//
LOCUS       KHUT01010651             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010651
VERSION     KHUT01010651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           54 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc cagagctcaa ctcggaaact gcttcgcata ctggcagact
      121 tgaggaatgc agaggtaagt agaattcctg gtgtagcggt gacatgcatt gatatcagga
      181 ggaataccca tggcgaaggc agcttactgg gcattatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010652             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010652
VERSION     KHUT01010652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           49 t
ORIGIN      
        1 gacgaactgt gcgaacgtta ttcggagtca ctgggcttaa agcgctcgta ggcggcattg
       61 caagtcggag gtgaaatact atcggctcaa ccgtagaatt gccctcgata ctgcaaagct
      121 agaggaaaat aggggcacgt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccgg tggcgaaagc gacgtgctgg gttttatctg acgctgagga gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010653             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010653
VERSION     KHUT01010653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           99 g           45 t
ORIGIN      
        1 gacgtaggag gcgagcgtta tccggattca ctgggcgtaa agggcatgta ggcggttgtg
       61 taagtcgtgt gttaaagccc ctggctcaac tgggggaggt catgcgagac tacactgact
      121 tgagggcagc agaggcacgt ggaactcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacacccg tggcgaaggc gacgtgctgg gctgcgactg acgctgaaat gcgaaagtgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010654             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010654
VERSION     KHUT01010654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           79 g           56 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcata
       61 gtaagcgaat agtgaaagcg tgtggctcaa ccatacatcc attatttgaa ctgctaagct
      121 tgaggatgag agaggttatt ggaattccca gtgtaggagt gaaatccgta gatattggga
      181 ggaacaccga tggcgtaggc agataactgg ctcattcctg acactaaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010655
VERSION     KHUT01010655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 tacgaaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcgcgta ggcggatccg
       61 taggtcgatt gtgaaatacc tgggctcaac ccgggaacgg cagtcgaaac cgcgtttctt
      121 gaatacctga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgcctggc agtgtattga cgctaaagtg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01010656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010656
VERSION     KHUT01010656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          104 g           37 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatggg
       61 caagtcggaa gtgaaattcc ggggctcaac cccggaccgg cttccgatac tgttcgtcta
      121 gggaccggta ggggccggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccggctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010657
VERSION     KHUT01010657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           87 g           52 t
ORIGIN      
        1 tacgagggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccta
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaggtg catttgaaac tgtagaactt
      121 gagatcgaga gaggaaagtg gaattacgag tgtagaggtg aaattcgtag atattcgtaa
      181 gaacaccagt ggcgaaggcg actttctggc tcgatactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010658
VERSION     KHUT01010658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           83 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggccgcgcgg
       61 gcagtcaaat gtgaaattca ggggctcaac ctctgcattg cgcttgatac ttccgtgctc
      121 gaaccttgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacacttag ggcgaaagca agactctgga caagtgttga cgctcatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010659
VERSION     KHUT01010659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          102 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggcgcgta ggcggcctct
       61 taagtcagtt gtgaaaggtc ccggctcaac cggggagttg cagctgaaac tgggaggctt
      121 gagtgcagga gaggagagcg gaattctcgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc ctgttactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010660
VERSION     KHUT01010660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          100 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatgtg
       61 caagtcgaag gtgaaatccc ggggctcaac cctgggactg ctctcgatac tgcatgtcta
      121 gggaccggta gaggtcggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccgactggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010661
VERSION     KHUT01010661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtctta
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg cattcagtac tgtgagacta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010662
VERSION     KHUT01010662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           93 g           45 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggttcgg
       61 taagtcggtt gtgaaatttc gcggcccaac cgcgaaactg cgtccgatac tgctgaactt
      121 gaggacggta gaggtcggtg gaattccagg tgtaccggtg aaatgggcag atatctggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagctag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01010663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010663
VERSION     KHUT01010663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtcaggt gtgaaatctc ggagctcaac tccgaaactg catttgatac tgccttgcta
      121 gagtactgga gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctgga cagtgactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010664
VERSION     KHUT01010664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgagca ggcggtttgg
       61 aaagttggac gtgaaatttt agggcttaac cctaaagggt cgtccaaaac tcccagacta
      121 gagtgcagga gaggaaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctttctggc ctgtaactga cgctgaggcg cgaaggctgg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010665
VERSION     KHUT01010665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttaga
       61 taggtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac catttgacta
      121 gagtatagga gagggtgtcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gacacctggc ctaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010666
VERSION     KHUT01010666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           86 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggcttcc
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaattg ccattgaaac tgtggagctc
      121 gagaacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcgtaactga cgctcatgca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010667
VERSION     KHUT01010667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          100 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttct
       61 caagtccggg gtgaaagctt tcggctcaac cggaaaagtg ccttggatac tggggagctc
      121 gagggaggta ggggcggccg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ggccgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010668
VERSION     KHUT01010668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagat gtgaaagccc cgggcttaac ctgggaattg catcttaaac tgcgtaactg
      121 gagtgcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010669
VERSION     KHUT01010669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           82 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgagtgta ggcggttatc
       61 tcagtctggt gtgaaagccc agggctcaac cctggaagtg cactggatac tagataacta
      121 gagggcgata gaggagatcg gaattaatgg tgtaggggtg aaatccgtag atatcattag
      181 gaacaccgaa agcgaaggct gatctctggg tcgtttctga cgctaagact cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010670
VERSION     KHUT01010670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggtgcgta ggcggctttt
       61 taagtcagat gtgaaagacc acagcttaac tgtggggttg catttgaaac tggagagctt
      121 gagtatcgga gagggtagtg gaattcctgg agtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg actacctgga cgaaaactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010671
VERSION     KHUT01010671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tggcggtctt
      121 gggccatcta ggggtcgccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggcgactgga gatgtgccga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010672
VERSION     KHUT01010672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 caagtcgggt gtgaaaaatc tgggctcaac ccagtggagc acccgatact gctgtgacta
      121 gagtccggta ggggagtgtg gaatttctgg tgtagcggtg aaatgcgcag atatcagaag
      181 gaacaccaac ggcgaaggca gcactctggg ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010673
VERSION     KHUT01010673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatta ttgggcgtaa agcgtacgca ggcggctcgg
       61 taagtcgagt gtgaaatccc gaggctcaac ctcggacgtg cattcgatac tgtcgagctt
      121 gaatgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacattga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010674
VERSION     KHUT01010674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 taagtccgat gtgaaatccc cgagctcaac ttgggaactg cattggaaac tggcgggctg
      121 gagtgtggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010675
VERSION     KHUT01010675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgccatg
       61 caggtcggat gtgaaagccc cgggctcaac tcgggaagtg cacccgaaac cgcatggcta
      121 gagtctcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaagactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010676
VERSION     KHUT01010676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           92 g           50 t
ORIGIN      
        1 gacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtctgt
       61 taagtagggt gtgaaagcct ctggctcaac cagagaatgg cgccctaaac tggcaggctt
      121 gagtgaggta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactgga ccttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010677
VERSION     KHUT01010677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcctgc
       61 taagtcagac gtgaaatccc tcggcttaac cggggaactg cgtctgagac tggcgggctt
      121 gagtacagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgttactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010678
VERSION     KHUT01010678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtccta
       61 tttgtctgag gtgaaatccc agggcttaac cctggaagtg ccttagaaac ggtaggacta
      121 gagttctaga ggggtttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc agcgaaggcg gcaaactgga tagacactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010679
VERSION     KHUT01010679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          100 g           40 t
ORIGIN      
        1 gacggagggt gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggtctgc
       61 taagtccggt gtgaaagccc ccggctcacc cggggaagtg cgccggaaac tggcagacta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010680
VERSION     KHUT01010680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           85 g           49 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggttcgg
       61 aaagtagggt gtgaaatccc acagctcaac tgtggaactg cgctctaaac ttccgggcta
      121 gagggatata gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010681
VERSION     KHUT01010681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gctaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 acagtcaggg gtgaaagccc gcggctcaac cgcggaagcg cctttgatac tgccggtctg
      121 gagggaggta ggggagtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcgctctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010682
VERSION     KHUT01010682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           59 c          105 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggcgcct
       61 tcagtcgggt gtgaaagccc ggggctcaac cccgggatgc cgcccgatac tggggcgctt
      121 gaggccggaa gaggggcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcgccctggt ccggttctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010683
VERSION     KHUT01010683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttttc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggaaggctc
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttgctgga ccaccactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010684
VERSION     KHUT01010684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtcatt
       61 cacgtcgggt gtgaaagctc ggggctcaac cccgagacgc cattcgatac gggatgacta
      121 gaggtaggta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gatctctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01010685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010685
VERSION     KHUT01010685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           83 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggaccgt
       61 taagtcagtg gtgaaagccc acagctcaac tgtggaattg ccattgatac tggcggtctt
      121 gagtacatgt gaagttggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacaccgat tgcgaaggca gctggctaac atgtaactga cgctgaggga cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010686
VERSION     KHUT01010686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           84 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgtgcgca ggcggatatg
       61 caagtaaggt gtgaaatctc tgggctcaac ctggaaagtg cgccttaaac tacatatcta
      121 gaatttcgga gagggaaggg gaatttctca tgtaggggta aaatccgtag atatgagaag
      181 gaacaccgga ggcgaaggcg ccttcctgga cgaatattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010687
VERSION     KHUT01010687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           87 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggtttgt
       61 gaagtcagat gtgaaagccc cgggcttaac ctgggatgtg cgtttgaaac tgacagacta
      121 gagtttgata gaggggggta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca gccccctgga ttaaaactga cgctcaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010688
VERSION     KHUT01010688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           82 g           58 t
ORIGIN      
        1 tacgagtggt gcaaacgtta ttcggaatca ttgggcgtaa agggcgtgta gacggtatgt
       61 ttagtctatt gttaaatcct tcggcttaac cgaagatcag cagtggatac tggcatgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010689
VERSION     KHUT01010689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           94 g           45 t
ORIGIN      
        1 gacgaaggtg gcgaccgttg ttcggaatca ctgggcataa agagctcgta ggcggtgaga
       61 aaagtagggt gtgaaatccc tcggcccaac cgaggaactg cgctctaaac tgtcttgctt
      121 gaggaccgaa ggggaccacg gaattcctgg tggagcggtg aaatgcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg gtggtctggt cggtttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010690
VERSION     KHUT01010690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           90 g           55 t
ORIGIN      
        1 tacagaggtt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttttt
       61 taagtgagat gtgaaatacc tgggcttaac ctgggggtgt catcttaaac tggaagacta
      121 gagtgaagga gagggtagta gaatttccgg agtagcggtg aaatgcgtag atctcggaaa
      181 gaataccggt ggcgaaggcg gctacctggc ctttaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010691
VERSION     KHUT01010691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           96 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgtta cccggattta ctgggcgtaa agggcgtgta ggcggttttc
       61 taagtccggg gctaaagacc agggctcaac cctgggtttg ccttggatac tggaaagctc
      121 gacggctgga gggggcagcg gaatttccgg agtagcggtg aaatgcgcag ataccggaag
      181 gaacgccaat agcgaaggca gctgcctgga cagtacgtga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01010692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010692
VERSION     KHUT01010692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggctgc
       61 caagttcggg gtgaaatcct ccggctcaac cggagaactg cctcgaatac tggcggtctc
      121 gaggggggta ggggcatgcg gaactatggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccccttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010693
VERSION     KHUT01010693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           45 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattca ttgggtttaa agggtgcgca ggcgggtctg
       61 taagcccgtg gtgaaagcct ggtgctcaac gccagaggtg ccgcgggaac tgtaggtctt
      121 gagtcgtgac gagggcggcg gaatgcgaag tgtagcggtg aaatgcttag atagttcgca
      181 gaacgccgat tgcgaaggca gccgcccagg gacgcactga cgctgaggca cgaaggcatg
      241 gggatcgaac agg
//
LOCUS       KHUT01010694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010694
VERSION     KHUT01010694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           57 c          111 g           37 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggttact
       61 cgcatcgtcg gtgaaagccc cccgctcaac gggggagggt ccggcgagat ggggtgactg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gctggctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010695
VERSION     KHUT01010695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           92 g           53 t
ORIGIN      
        1 gacgtaggtt gcgagcgtta ttcggattca ctaggcgtaa agcgtgtgta ggtggatgcc
       61 taagtctgac gtgaaatctc ccggcttaac tgggaggggt cgttagatac tgggcgtctt
      121 gagttgggta gggggaaccg gaattccagg tgtagcggtg aaatgcgcag atatctggag
      181 gaacaccggc ggcgaaggcg ggttcctggg ccttaactga cactgagaca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010696
VERSION     KHUT01010696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           90 g           46 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agagcacgta ggcggctttg
       61 caagtcggat gtgaaagccc cgggcttaac ccgggaatag cacccgaaac tacaaagctt
      121 gaatgcttga gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcgga ggcgaaggcg gccaactggc gatgcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010697
VERSION     KHUT01010697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtgtgt
       61 taagtcggcg gtgaaatact tcggctcaac cggagaatgg ccgctgaaac tggcacgctc
      121 gagggaggta gaggtgcgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacacctgt ggcgaaagcg acgcactgga ccttttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010698
VERSION     KHUT01010698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcaggg
       61 taagtctgat gtgaaatctc cgagcctaac tcggaaatgg cattggaaac taccttgctt
      121 gagggttgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010699
VERSION     KHUT01010699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           52 c           81 g           49 t
ORIGIN      
        1 gacgtaccac ccaaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggccaag
       61 taagttgggt gtgaaatacc tcagctcaac tgaggaattg cgctcaaaac tacttggctc
      121 gagaaagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcattg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010700
VERSION     KHUT01010700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggtggccggg
       61 ccagtcaggc gtgaaagccc tgggcttaac ccaggaagtg cacctgatac tgctcggctc
      121 gagtacggga gagggtagcg gaattcccgg tgtagaggtg aaattcggag atatcgggag
      181 gaacatcagt ggcgaaggcg gctacctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010701
VERSION     KHUT01010701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttcgc
       61 atagtcgggg ctgaaatctt tcggcttaac cggaaaagtg gcttcgatac tggcggactg
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010702
VERSION     KHUT01010702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           83 g           53 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtatctt gtgaaatccc atggctcaac catggaactg cttgataaac tggatgtctt
      121 gagccactta ggggtcagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctgactggg agtgtgctga cgctgagaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010703
VERSION     KHUT01010703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ctgggcataa agcgtgcgta ggcggccaga
       61 tcagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac tgtctggctt
      121 gaggtccgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cggatcctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010704
VERSION     KHUT01010704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta gggggcttgg
       61 caagtcagat gtgaaatctc accgcctaac ggtgaaactg catttgatac tgccgagcta
      121 gaggatcgga gaggagaacg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcaat tgcgaaggca gttctctgga cggttcctga ccctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010705
VERSION     KHUT01010705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           52 c           75 g           50 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agcgcgagca gccggtcgta
       61 ttagtctgat gtaaaagcac agggcccaac tctgtgtccg cgttggaaac ggtacgactt
      121 gagcgaataa gaggagagca gaacgtatag tgtaggggtg aaatccactg atattatacg
      181 gaataccaaa agcgaaggca gctctctagt gttctgctga cggtcaatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01010706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010706
VERSION     KHUT01010706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggctccg
       61 catgtcgggc gtgaaatccc cgggcttaac ctgggaatgg cgtccgaaac tatggggctg
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010707
VERSION     KHUT01010707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggtata
       61 caagtttgat gtgaaatccc tgggcttaac ctgggaacgg cgttgaatac tgtagatcta
      121 gagtctggca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg tcggcctggg ccaagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010708
VERSION     KHUT01010708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaataa ctgggcgtaa agggtgcgta ggtggatgtg
       61 tgcgttagat gtgaaatccc cgggcttaac ctgggaactg catttaatac ggcacatcta
      121 gagtgcgaga ggggaaagcg gaatggcgag tggagcagtg aaatgcattg agattcgcta
      181 gaacaccaga ggcgaaggcg gctttctggc tcgctactga cactaaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010709
VERSION     KHUT01010709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcctgg
       61 tcagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgatac taccaggctt
      121 gagggagata ggggcgtgtg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacgctggg tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010710
VERSION     KHUT01010710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           98 g           44 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ctgggcgtaa agggcacgca ggcggctcgg
       61 caggtagggc atgaaatctc ccggcttaac tgggaggggc tgtcctaaac cgctgggctt
      121 gagggcggga gagggaagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg acttcctggt ccgcccctga cgctcatgtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010711
VERSION     KHUT01010711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           52 c           72 g           52 t
ORIGIN      
        1 tcccagcagt ccaagtcgca atcacaatta ttgggtctaa aacatccgta gcttgcttcg
       61 caagtctctt gtgaaatccg gcctcttaag ggtcgaagtg cgagagatac tgcaaagcta
      121 gagaccggga gaggtaagaa gtacaaacag ggtagcggta aaatgtgttg atcccgtttg
      181 gactaacaac agcgaaggca tcttactaga acggatctga cagtgaggga tgaaggctag
      241 gggcgcaaaa cgg
//
LOCUS       KHUT01010712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010712
VERSION     KHUT01010712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           83 g           55 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattca ctgggcgtaa agcgcgtgca ggcggttcgg
       61 taagttgggt atgaaatctc ctggcttaac taggagaggt tatccaatac taccggactt
      121 gagaatggta gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg atcttctgga ccatttctga cgctcagacg cgaaagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01010713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010713
VERSION     KHUT01010713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           94 g           44 t
ORIGIN      
        1 tacggaggtt gcgagcgttg ttcggaatca ctgggcgtaa agggagcgca ggcggtcagg
       61 tgtgtctgaa gtgaaatccc gaggctcaac ctcggaactg cttcggatac tgcctgactt
      121 gagtacgcga gaggtgggcg gaattccagg tgtagcggtg aaatgcgcag atatctggag
      181 gaacacccgt ggcgaaagcg gcccactggc gcgaaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010714
VERSION     KHUT01010714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           85 g           48 t
ORIGIN      
        1 gacgagccac ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaacag cgctctaaac taccgtgctt
      121 gagggagata gaggtaagcg gaacttcggg tggaacggtg aaatgtgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactgga tcttctctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010715
VERSION     KHUT01010715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           76 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggtctg
       61 taagtcagcg gtgaaatctc cgagcttaac tcggaaactg ccattgatac tataggtctt
      121 gaataccgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca cgcatattga ctctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010716
VERSION     KHUT01010716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttaaa
       61 caagtcaggc gtgaaagccc cgggctcaac ccgggaatag cgcttgagac tgtttttcta
      121 gagttcggga gaggtaagtg gaattcccag tgtagcagtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gcttactgga ccgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010717
VERSION     KHUT01010717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcggga
       61 taagtcgcat gtgaaagccc tcggctcaac cgaggaagtg catgcgatac tgtttcgctt
      121 gaatgccgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggcaattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010718
VERSION     KHUT01010718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgt
       61 taagtctcgt gtgaaagccc tgagctcaac tcaggaagtg cgcgagatac tggcgagctg
      121 gagtgacgga gaggatggcg gaatacccgg tgtagaggtg aaattcgtag atatcgggtg
      181 gaacacctgt ggcgaaggcg gccatctgga cgtcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010719
VERSION     KHUT01010719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           91 g           50 t
ORIGIN      
        1 tacgtagggt gcgaacgttg ttcggattta ctgggcataa agagctcgta ggcggcgcca
       61 taagtcggtt gtcaaatccc ctggctcaac cagggaactg ctgccgatac tgcggtgctt
      121 gagtgtgaga ggggagagtg gaacgtgcag tggagcggtg aaatgcgttg atattgcacg
      181 gaacgccggt ggcgaaagcg actctctggc tcactactga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010720
VERSION     KHUT01010720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           54 c           79 g           49 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ctgggcgtaa agcgtccgca ggtggttcgt
       61 taagtcatct gtcaaatccc ggtgctcaac accggaactg cgggtgatac tggcgaactt
      121 gaggctggaa gaggtaaggg gaactgctgg tgtagtagta aaatgcgtta atatcagcag
      181 gaacaccaaa ggcgaaggca ccttactggg acagtcctga cactcaggga cgaaagcgtg
      241 gggagcaaaa cgg
//
LOCUS       KHUT01010721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010721
VERSION     KHUT01010721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           97 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctc
       61 caagtctgtg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tggggatctc
      121 gaggagggta ggggcatctg gaacagccgg tggagcggtg aaatgcgttg atatcggctg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010722
VERSION     KHUT01010722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           75 g           58 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggcgggtttg
       61 taagtcagtg gtgaaagccg gtcgctcaac gatcgaactg ccattgatac tgcagaccta
      121 gaatatagat gatgttggcg gaatatgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccgat tgcgaaggca gctgacaaaa ctattattga cgctgatgca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010723
VERSION     KHUT01010723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcttgt
       61 caagtcaggg gtgaaatcct ggagctcaac tccagaactg cctctgaaac tgccaagctt
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010724
VERSION     KHUT01010724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgagtggt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgctatg
       61 aaggtctgat gtgaaatacc acggctcaac cgtggaacgg cgttggaaac cacatggctg
      121 gagtgagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctttactga cgctgatgca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010725             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010725
VERSION     KHUT01010725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcggctttg
       61 acagtcgaag gtgaaatccc accggctcaa ccgtggaatt gccttcgata ctacaaggct
      121 tgagagagct aggggcatgt ggaacttccg gtggagcggt gaaatgtgtt gatatcggag
      181 ggaacgccag tggcgaaagc ggcatgctgg atctcaactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010726             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010726
VERSION     KHUT01010726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttag
       61 aaagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac ttctatgact
      121 tgagtttggt aggggtaagc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agcttactgg gccaaaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010727
VERSION     KHUT01010727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           63 c          102 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctac
       61 cgcgtccggg gtgaaatccc gccgctcaac ggcgggactg cctcggatac gggtggcctc
      121 gaggggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccttcactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010728
VERSION     KHUT01010728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           87 g           52 t
ORIGIN      
        1 tacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggatgg
       61 taagtacttt gtgaaatccc acggcttaac cgtggaattg cttggtatac tgccgttctt
      121 gagtcatcta ggggcagccg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgcagaca ggctgctggg gatgtactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010729
VERSION     KHUT01010729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcctc
       61 taagtcagtt gtgaaatatc ccggcttaac cgggagggtg cgattgatac tggaggactt
      121 gagtcacagc gaggttggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gccaactagg ggtgaactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010730
VERSION     KHUT01010730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           97 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 acagtcaggg gtgaaagcct ccagctcaac tggagaagtg cctttgatac tggcggcctc
      121 gaggggtgca ggggcgtgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacaccggt ggcgaaggcg gcacgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010731
VERSION     KHUT01010731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatta ttgggcgtaa agcgtatgta ggcggccatc
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaaggg catttgatac tggatggctt
      121 gaatatggga gaggatagtg gaattcttgg tgtagtggtg aaatacgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gctatctggc cctatattga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010732
VERSION     KHUT01010732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           82 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttttt
       61 taagtcagag gtgaaagtct gcggctcaac cgtaggattg cctttgatac tgagaaactt
      121 gagtacagtt gaggtaggcg gaattgacag tgtagcggtg aaatgcttag atattgtcaa
      181 gaacaccgat tgcgaaggca gcttactaag ctgaaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010733
VERSION     KHUT01010733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           83 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaataca gcagcttaac tgttgaggtg ccattgatac tgaagagctt
      121 gagtacagat gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gcttactaag ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010734
VERSION     KHUT01010734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           82 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctcta
       61 taagtcagcg ttgaaatatg gcagctcaac tgtcagactg gcattgatac tgtagagctt
      121 gagtgaagtc gaggttggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat tgcgaaggca gctaactaga ctttaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010735
VERSION     KHUT01010735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggtgcag
       61 taagttggaa ttgaaagccc ggggctcaac tccggacccg gttccaaaac tattgtacta
      121 gagcggatca gaggacagcg gaattcccgg tgtagcggta atatgcgtag atatcgggag
      181 gaacaccagt ggcgtaggcg gctgtctggg gtcttgctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010736
VERSION     KHUT01010736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ctcggattca ctgggtgtaa agggcgtgta ggcggtttcg
       61 taagccttgt ttaaaaggct ctggctcaac cagagtttgt gacaggggac tgcgagactt
      121 gaggatggga gaggtgagcg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccattcctga cgctgagatg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010737
VERSION     KHUT01010737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           98 g           35 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtcaga
       61 caagtgggct atgaaagcgc ccggctcaac cgggcgaggc tagtccaaac tgtttgactg
      121 gagggcagca gaggtgtgtg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcacactggg ctgcaactga cgctgaaccg cgacagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010738
VERSION     KHUT01010738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           89 g           44 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggcccga
       61 caagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacggatac tgtcgggcta
      121 gagtgtggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtacctgga ccactactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010739
VERSION     KHUT01010739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttatt
       61 taagtcaggt gtgaaattcc cgagctcaac ttggggactg cgcctgatac tggatagcta
      121 gagtcctgta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaggcg actttctgga cagagactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010740
VERSION     KHUT01010740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggccgat
       61 taagtcgcat gtgaaagccc agggctcaac cctggaagtg catgcgatac tgattggcta
      121 gagtcctgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg accgcctgga caggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010741
VERSION     KHUT01010741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           91 g           48 t
ORIGIN      
        1 aacgtaggaa gcgagcgtta tccggattta ctgggcgtaa agggcatgca ggcggtctgt
       61 caagttggag ttgaaagctc tcggctaaac tgggagaggt gcttcaagac tgacagacta
      121 gagtatggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccataactga cgctcagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01010742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010742
VERSION     KHUT01010742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggcgcca
       61 taagttggag gtgaaatcct gcggctcaac cgtagaatag cccccaaaac tgtggcgctt
      121 gaggaaggta ggggtctgcg gaactctcgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccagc ggcgaaagcg gcagactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010743
VERSION     KHUT01010743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacgtagacc acaagcgtta tccggattta ttgggcgtaa agagcatgta ggaggttcgg
       61 tgcgtctctg gttaaagccc accgcctaac ggtggaagtg ccggagatac gatcggacta
      121 gaggaggtga gaggtgcatg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca gtgcactggt accttcctga ctctgagatg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01010744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010744
VERSION     KHUT01010744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           84 g           47 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgtctttc
       61 cacgtctgac aggaaattgc ggggctcaac cccgcatcgc tgttggaaac gagaaagatg
      121 gagtcatgca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaagca ggatgctggg catgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01010745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010745
VERSION     KHUT01010745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtctgc
       61 taagtttgat gtgaaatccc ggggcttaac cctggaatgg cattgaatac tggcggacta
      121 gagtcctgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga caggtactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010746
VERSION     KHUT01010746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 gacagaggtt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggcggaa
       61 tgtgtctgat gtgaaatccc tcggcttaac tgaggaagtg catcggaaac tgtttcgctg
      121 gagtgctaga gaggatggcg gaattcctag agtagaagtg aaattcgtag atactaggag
      181 gaacacctga ggcgaaggcg gccatctggc tagcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010747
VERSION     KHUT01010747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggcttcg
       61 caagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgaaac tgcgaggctt
      121 gaatcttgga gagggcagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actgcctgga caaagattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010748
VERSION     KHUT01010748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           94 g           38 t
ORIGIN      
        1 gacagaggcc ccaagcgtta gtcggaatta ctgggcttaa agggtgcgta ggcgggcgtg
       61 aaagtgttct gtgaaatccc cgggctcaac ccgggaaccg cagggcatac tccacgtctc
      121 gaggaagcta gaggccggcg gaacgatcgg tggagcggtg gaatgcgtag agatcgatcg
      181 gaacgccaag ggtgaagaca gccggctggg gctttcctga cgctgaggca cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01010749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010749
VERSION     KHUT01010749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttga
       61 taagtcagac gtgaaatccc tcagcttaac tgaggaattg catctgatac tgtcagactt
      121 gagtatcgga ggggatggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010750
VERSION     KHUT01010750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           91 g           58 t
ORIGIN      
        1 gacgtaggat gcaagcgttg ttcggattta ttgggcgtaa agcgtacgta ggcggctgtt
       61 taagtctggt gttaaagcta gaggctcaac ctctgtgttg cactggatac tgggcagctt
      121 gagaacggta ggggaaggtg gaattccgag tgtagcggtg aaatgcgtag atattcggaa
      181 gaacaccggt ggcgaaggcg accttctggg ccgtttctga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010751
VERSION     KHUT01010751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           94 g           41 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggatcg
       61 taagtcaggg gtgaaatccc acggctcaac cgtggaagtg cctttgatac tgcggacctc
      121 gaggtgggca ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcacgctggg cccgtactga cgctgaggcg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010752
VERSION     KHUT01010752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           97 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggattcg
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaagtg ccttggatac tgcgagtctc
      121 gagggaggta ggggccgcgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cgcggctgga tctcctctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010753
VERSION     KHUT01010753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           84 g           55 t
ORIGIN      
        1 tacagggggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcacgta ggtggtttgt
       61 taagttcgat gtgaaatccc tggactcaat tcaggaagtg cattgaatac tggcagacta
      121 gagtatagga ggggtaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacagt ggcgaaggcg gcttactgga ctataactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010754
VERSION     KHUT01010754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           93 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 ccagtcaggg gtgaaatcct ccagcttaac tggagaacag cccctgatac tgaccacctc
      121 gaggggtgta ggggcgtgcg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg gcgcgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010755
VERSION     KHUT01010755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c          102 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcgcga
       61 caagtcgggg gtgaaatctt ccggcccaac cggaaaaggg cccccgatac tggcgtgctg
      121 gagggaggta ggggcgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga cctcacctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010756
VERSION     KHUT01010756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           55 c           78 g           51 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggttcgt
       61 tcagtcgtct gtcaaatccc ggggctcaac cccggaactg cagacgatac tggcggactt
      121 gagaccggta gaggcaagcg gaattacagg tgtagcggta aaatgcgttt atatctgtaa
      181 gaacaccaaa ggcgaaagca gcttgctgga acggttctga cactcatgga cgaaagcgtg
      241 gggagcgaaa agg
//
LOCUS       KHUT01010757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010757
VERSION     KHUT01010757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggttcg
       61 taagtcgatt gtgaaagccc cgggctcaat ccgggaattg cagtcgaaac tacgaatctt
      121 gaatacctga gagggtgacg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gttgcctggc agtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010758
VERSION     KHUT01010758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           93 g           41 t
ORIGIN      
        1 gacagaggag gcaagcgttg ttcggaatta ctgggcttaa agcgcgcgta ggcggctcgg
       61 aaagtgtcct gtgaaatccc gtcgctcaac gacggaactg cggggcaaac tcccgagctt
      121 gaggttggta gaggtgcctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaga agcgaaagcg gggcactggg ccaaatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010759
VERSION     KHUT01010759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           83 g           55 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggattta ttgggcgtaa agagttcgta ggcggttttg
       61 taagtcattt gttaaatcct caggcttaac ttgggggctg cgggtgatac tgcaaggcta
      121 gagagtggga gaggcaagcg gaatgcccgg tgtagcggta aaatgcgtta atatcgggta
      181 gaacaccaaa ggcgaaggca gcttgctgga acacttctga cgctcaagaa cgaaagcgtg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01010760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010760
VERSION     KHUT01010760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcaaga
       61 taagtcgggt gtgaaatccc ttggcttaac caaggaagtg cattcgatac tgtctcgctt
      121 gagtaccgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggacactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010761
VERSION     KHUT01010761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           88 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggtggtatat
       61 caagccgtat gtgaaatccc cgggcttaac ctgggaatgg cctatgggac tggtatacta
      121 gagtacggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ttgatactga cactcaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010762
VERSION     KHUT01010762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttcgt
       61 taagtcagaa gtgaaagcgc tgggcctaac ccagcaacgg cttttgagac tggcgagcta
      121 gagggtccta ggggatgctg gaattccagg tgtagcggtg aaatgcgtag atatctggga
      181 gaacacccgt ggcgaaggcg ggcatctggg ggacacctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010763
VERSION     KHUT01010763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          100 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctctg
       61 taagtcggac gtgaaagtcc agggctcaac cctgggatgc cgttcgatac tgcagggcta
      121 gaggcaggta ggggaaagtg gaatacctgg tgtagcggtg aaatgcgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg gctttctggg cctcgcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01010764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010764
VERSION     KHUT01010764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           87 g           50 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgcgaatctt
      121 gggccatcta ggggcgagtg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca actcgctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010765
VERSION     KHUT01010765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           37 c           80 g           62 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggtggttttg
       61 taagttagat gtgaaagccc agggcttaac cctggaattg catttaagac tgcattactt
      121 gaaggtcaaa gaggtaagca gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaataccgat ggcgtaggca gcttactggt tgacacttga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010766
VERSION     KHUT01010766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           96 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgtg
       61 cgagtcgggt gtgaaagccc cgggcttaac ctgggaattg cattcgatac tgtacggcta
      121 gagtctagga gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg acttcctggc ctaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010767
VERSION     KHUT01010767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggtggttgga
       61 taagtccgta atgaaatctc ccggcttaac tgggaggggt ttagggatac tgtccggctt
      121 gagtgaggga gaggtaactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaac ggcgaaagca ggttactggc cctatactga cattgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010768
VERSION     KHUT01010768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agggttcgta ggcggccttg
       61 taagtggggt gtgaaatccc tcggctcaac cgaggaactg cgctccaaac tacaaggctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010769
VERSION     KHUT01010769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           65 c          108 g           30 t
ORIGIN      
        1 cacgtgggag gcgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcgacctcg
       61 caggcagtcc gtgaaagtcc ccggctcaac cggggagggt cgggctgaac cgcgaggctg
      121 gaggccggca gaggcgggtg gaattcccgg aggagcagtg atatgcgtag ataccgggag
      181 gaacgcccga ggtgaagacg gcccgctggg ccggtcctga cgctgaagcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010770
VERSION     KHUT01010770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           81 g           52 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgtctgta ggtgacttcc
       61 cacgtctggt tttaaatttc ggggcttaac cccgtcttcg aactggaaac gaggaggatt
      121 gagtcattca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaggca ggatgctagg aatgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01010771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010771
VERSION     KHUT01010771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacggt
       61 caagtcggtg gtgaaatcgt tcggcccaac cggacaagtg ccgccgatac tggccgtctc
      121 gagggaggta ggggtcgagg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010772
VERSION     KHUT01010772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           95 g           43 t
ORIGIN      
        1 gacgaaccga gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcatc
       61 caagtccgtg gtgaaatccg gcagctcaac tgtcgaattg ccgcggatac tgggtgtctc
      121 gagggaggta ggggcgcgcg gaactaacgg tggagcggtg aaatgcgttg atatcgttag
      181 gaactccggt ggcgaaggcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010773
VERSION     KHUT01010773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           85 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaga
       61 ttagttggat gtgaaatact cgggcttaac ctgggaactg cattcaatac tgtctagcta
      121 gagttttgta gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acctcctgga caaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010774
VERSION     KHUT01010774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgagtgta ggcggccaga
       61 aaagtcagtt gtgaaagccc cgggctcaac ccgggaagtg cttctgatac tgtttggctt
      121 gagtactaga gagggtagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctacctggc tatgtactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010775
VERSION     KHUT01010775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggaata
       61 aaagtttgat gtgaaagccc caggcttaac ctgggaatgg cgttgaaaac tgtatttcta
      121 gagtcggaca gagggccgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcggcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010776
VERSION     KHUT01010776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           84 g           50 t
ORIGIN      
        1 gacagaggtc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatagg
       61 taagtgcttt gtgaaatccc acggcttaac cgtggaactg ctgagcatac tgcccgtctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctgga ggtgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010777
VERSION     KHUT01010777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           78 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggataag
       61 taagtccgtg gtgaaatcct gcagcttaac tgcagaactg ccgtggatac tatttatctt
      121 gaatgtcgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctagctacg cggatattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010778
VERSION     KHUT01010778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacgtagggg ccaagcgttg tccggaatta ctgggcgtaa agcgtgggta ggtggtcggt
       61 gatgtgccgc gtgaaagcgc cggagtaatg ccggcgaggt cgcggtagac acattgactt
      121 gaggctcgca gaggaacgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgcaagcg gcgttctggg cgagacctga cactgagcca cgacggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010779
VERSION     KHUT01010779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggacttt
       61 taagtcagcg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tattagtctt
      121 gaatattgtt gaggtgggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gctcactaag caatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010780
VERSION     KHUT01010780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctcg
       61 caagtcggtc gtgaaagccc ttggcttaac caaggaactg cgatcgaaac tacgggacta
      121 gagtatcgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg accatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010781
VERSION     KHUT01010781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agagcgtgta ggcggcttga
       61 aaggtccgct gtgaaaactc gaggctcaac ctcgagacgt cggcggaaac catctggcta
      121 gagtccggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010782
VERSION     KHUT01010782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           88 g           57 t
ORIGIN      
        1 tacgtaggtc ccgagcgttg tccggaatta ctgggtgtaa agggtgcgca ggcggttttg
       61 tgcgtcagaa gtgaaatatc acggctcaac cgtgagggtg cttttgatac ggcaagactt
      121 gagtacgaga gaggatgata gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaggcg gtcatctggc tcgttactga cgctcaggca cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010783
VERSION     KHUT01010783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           55 c           75 g           49 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agcgcgagca gccggttgtg
       61 ttagtctgat gtaaaaccat ggggcccaac tccatgtctg cgtcggaaac ggcacacctt
      121 gagcgaataa gaggagagca gaacgtatgg tgtaggggtg aaatccactg atatcatacg
      181 gaataccaaa agcgaaggca gctctctagt gttctgctga cggtcaatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01010784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010784
VERSION     KHUT01010784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           45 c           80 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggagata
       61 atagtcagat gtgaaatcct ggggctcaac ctcagaacag catttgaaac tgtatctctc
      121 gaggatagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01010785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010785
VERSION     KHUT01010785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta ctcggaatca ctaggcgtaa agcgcatgta ggcggttggt
       61 caagtccgtc gtgaaatctc catgctcaac gtggagcggt cgacggatac tgatcagctg
      121 gagtttggga gaggtgactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaat ggcgaaagca ggtcactgga ccaatactga cgctgagatg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010786
VERSION     KHUT01010786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          109 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtttgg
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcggac tgcgagactg
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010787
VERSION     KHUT01010787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtctgc
       61 taagtcgggt gtgaaatccc tcggctcaac cgaggaattg cacccgatac tggcaggctc
      121 gagggtggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccacatctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010788
VERSION     KHUT01010788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          106 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggttggc
       61 agagcccggc gtgaaagctc ccggcttaac cgggagaggt cgtcggggac ctgttgactg
      121 gaggccggta ggggagagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccggtcctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01010789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010789
VERSION     KHUT01010789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           76 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtttat
       61 taagtcagtg gtgaaagttt gcggctcaac cgtaaaattg ccattgaaac tgatagactt
      121 gaatttggtt gaggttgccg gaatgtgtca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca ggtaactaaa ccaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010790
VERSION     KHUT01010790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggtcttc
       61 taagtctgat gtgaaagccc aaggcttaac cttggaagtg cattggatac tggaagacta
      121 gagggcgaga ggggacattg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg aatgtctggc tcgtttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010791
VERSION     KHUT01010791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           94 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtccgct gtaaaagtca agggctcaac ccttgaaggc cggtggaaac tgccggacta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010792
VERSION     KHUT01010792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          101 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgg
       61 taagttcggg gtgaaagccc gcggctcaac cgcggaagcg cctcggatac tgccggcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010793
VERSION     KHUT01010793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctatc
       61 taagtctggt gtgaaagccc tcggcttaac cgaggaagtg cattggatac tggatagcta
      121 gaggatgaga gaggagagta gaattcccgg tgtaggggtg aaatccgtag agatcgggag
      181 gaataccaga ggcgaaggcg gctctctggc tcatatctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010794
VERSION     KHUT01010794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           83 g           54 t
ORIGIN      
        1 gacgagggac cctagcgttg ttcggaatca ttgggcgtaa agcggatgta ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtggtgga taggtaaaca gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccgga ggcgaaggcg gtttactggc cacacactga cactcagatc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01010795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010795
VERSION     KHUT01010795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           78 g           62 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcaggg gtgaaatatt tcggcttaac cggaatcttg cctttgatac tgctaggctt
      121 gaatgtagat gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttactaag ttacaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010796
VERSION     KHUT01010796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           88 g           56 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttttg
       61 caagtcagtg gtgaaatccc ggagcttaac tccggaattg cttttgatac tgcaaggcta
      121 gagtgtgtta gaggatggcg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg acacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010797
VERSION     KHUT01010797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggacggc
       61 caagtcaggc gtgaaatccc cgggctcaac ccgggaactg cgtctgaaac tggtcgtctc
      121 gagtgtcgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010798
VERSION     KHUT01010798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           86 g           52 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 catgtcttat gtgaaagccc agagcttaac tctggaagtg cataagaaac tgttgaattt
      121 gagtatagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagc ggcgaaggcg gccttctgga ctataactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010799
VERSION     KHUT01010799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggacaag
       61 caagtcgggt gtgaaatccc tgggctcaac ccaggaactg catccgaaac tgcatgtctt
      121 gagtgtcgga gagggaagtg gaattccagg tgtaggggtg aaatccgtag atatctggag
      181 gaacatcagt ggcgaaggcg gcttcctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010800
VERSION     KHUT01010800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggttgtattg
       61 agagtcacgg gtgaaatccc caagcttaac ttgggaactg cctgtgagac ctcagtacta
      121 gagtatgaga gaggattgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg acaatctggc tcataactga cactcaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010801
VERSION     KHUT01010801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agcgcacgca ggcggcggct
       61 taagtcggat gtgaaagccc ggggctcaac cccggaattg cattcgaaac tgggtcgctt
      121 gaatacggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaagcg gcttcctggc ccgatattga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010802
VERSION     KHUT01010802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgcca
       61 taagtcggat gtgaaatctc tgggctcaac ccagaaattg cattcgatac tgtggtgctc
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010803
VERSION     KHUT01010803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agggcgcgta ggcggaaatt
       61 gaagtcagat gtgaaatttc ggggctcacc cccggagctg catttgaaac tctttttcta
      121 gagggtaggc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01010804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010804
VERSION     KHUT01010804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaga
       61 tgggtgagat gtgaaagccc tgggcttaac ctgggaagtg cattttaaac tatttagctg
      121 gagtacagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacgtcaat ggcgaaggca gccacctgga ctgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010805
VERSION     KHUT01010805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggtggttcgt
       61 taagtcagat gtgaaattcc ggggctcaac cccggagctg catctgaaac tgacgaacta
      121 gagttcagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gttttctagg ctgatactga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010806
VERSION     KHUT01010806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           90 g           58 t
ORIGIN      
        1 tacagaggtg gcaaacgttg ttcggattta ctgggcgtaa agggtgcgta ggtgggttcg
       61 tatgtcgggt gtgaaatctc actgctcaac ggtgaaattg cattcgaaac tgcggatctg
      121 gagtgtatga ggggagagcg gaattcttgg tgtagcggtg aaatgcgttg atatcaagaa
      181 gaacaccggt ggcgaaggcg gctctctggc atgctactga cactgaggca cgaaagttgg
      241 gggagcaaac agg
//
LOCUS       KHUT01010807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010807
VERSION     KHUT01010807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 cacgtaggag gcgagcgtta ttcggattta ctgggtgtaa agcgcgtgta ggcggcgcag
       61 aaagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaaaac ttctgtgctt
      121 gagtatggta gaggaaaatg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg attttctgga ccaagactga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010808
VERSION     KHUT01010808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 aacgtaggag gctagcgtta tccggattta ttgggcgtaa agcgcgtgca ggcggtttga
       61 taagttggat gtgaaatctc ccggcttaac tgggagcggt cgttcaatac tatcagacta
      121 gagattggga gagggaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctaga ccagttctga cgctaagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010809
VERSION     KHUT01010809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 tacgtatggg gcaagcgtta tccggattca ttgggcgtaa agagcgcgta ggcggcttgt
       61 ttagcgagat gtgaaaaccg ggggctcaac ccgcggcctg catctcgaac tggcaggctt
      121 gagtctggta gaggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01010810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010810
VERSION     KHUT01010810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           80 g           51 t
ORIGIN      
        1 tacgagggcc tcaagcgtta tccggaatca ctgggcgtaa agggtccgta ggtggcagtg
       61 ttagtctctt gttaaatatc ctcgctcaac ggggaaactg caagggaaac ggcacagctc
      121 gaggatgtaa gaggtgtaca gaactctcgg tgtaggggtg aaatccgttg atatcgaggg
      181 gaataccaaa tgcgaaggca gtacactggt acactcctga cactgaagga cgaaaccctg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010811
VERSION     KHUT01010811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggtttgg
       61 ttagtcagac gtgaaagccc tgggcttaac ccaggaactg cgtttgatac tgccaagcta
      121 gagtgcgaga gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccaga ggcgaaggcg gctttctggc tcgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010812
VERSION     KHUT01010812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           95 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtttgt
       61 taagttagct gtgaaagccc cgggcttaac ctgggaaagg cagttgagac tggcaggcta
      121 gagtacaaga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctcactggc ttgatactga cgctgaggtg cgaaggcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010813
VERSION     KHUT01010813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           95 g           40 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggacttg
       61 taagtcggga gtgaaaactc ggggctcaac cccgagcctg cttccgaaac tgcgagtctt
      121 gaggaccgga gaggaaggcg gaattcccag tgtagcggtg gaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccttctgga cggtacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010814
VERSION     KHUT01010814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           95 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agagcgtgta ggcggctcgc
       61 taagtcgact gtgaaagccc tcggctcaac cggggaagtg cagtcgatac tggcaagctc
      121 gagtcccgaa gagggtggcg gaatgcgcag tgtagaggtg aaattcgtag atattgcgta
      181 gaacatcggt ggcgaaggcg gccacctggg aggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010815
VERSION     KHUT01010815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           97 g           38 t
ORIGIN      
        1 tacgaggggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgca ggcgggcctg
       61 caggcgtctt gtgaaatccc ccggctcaac cggggaattg cagggcgaac cgcaggtctt
      121 gaggaaggca gaggcgggtg gaaccatagg tggagcggtg gaatgcgtag agatctatgg
      181 gaacgccgaa ggtgaagaca gcccgctggg cctttcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010816
VERSION     KHUT01010816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           83 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctaat
       61 taagtcagtg gtgaaagcct gtagctcaac tatagaattg ccattgatac tggttagctt
      121 gagttcggtt gaggtaagcg gaatggatgg tgtagcggtg aaatgcatag atatcatcca
      181 gaacaccgat agcgaaggca gcttactggg ccgaaactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010817
VERSION     KHUT01010817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttatt
       61 taagttatct gtgaaatacc tgggcttaac ctgggggtgt cagataagac tggatagcta
      121 gagtatagga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actgcctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010818
VERSION     KHUT01010818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttatt
       61 taagttaggt gtgaaatccc cgggcttaac ctgggaatgg catttaagac tgagtagcta
      121 gagtttggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccatcactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010819
VERSION     KHUT01010819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           84 g           56 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggcgcgta ggcggattag
       61 caagtcagag gtgaaatctc ccggcttaac cgggaaactg cctttgaaac tgttaatctt
      121 gagtacggaa gagagaagca gaattccagg tgtagtggtg aaatacgtag atatctggaa
      181 gaataccagt tgcgaaggcg gcttcttggt ccgttactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010820
VERSION     KHUT01010820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           82 g           54 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcagaatta ctgggcgtaa agcgcacgta ggtggatatt
       61 ttagtcgggt gtgaaagccc cgggcttaac ccgggaattg cattcgatac tgaatatcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttcctgga ctaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010821
VERSION     KHUT01010821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           82 g           58 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggattta ttgggcgtaa agcgtcagca ggttctttac
       61 cgcatcttgt ttcaaatacc agagctcaac tttgggcagg gacaagagat gggtagagtt
      121 gaaatactca ggggctactg gaacttatgg tgtaggagtg aaatccgttg atatcataag
      181 gaacgtcaag ggcgaaggca ggtagctggg ggatatttga cactaagtga cgaaagccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01010822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010822
VERSION     KHUT01010822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           88 g           46 t
ORIGIN      
        1 tacggagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggtcgtg
       61 tgtgtcaggt gtgaaatctc ggagctcaac tccgaaactg cgcctgaaac tacacggcta
      121 gagtatcgga gaggtgagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gctcactgga cgattactga cgctcaggca cgaaagcgtg
      241 gggagccaaa ggg
//
LOCUS       KHUT01010823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010823
VERSION     KHUT01010823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           82 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtagcc
       61 taagtcagtt gtgaaatacg atagcttaac tatcgaggtg caattgatac tgggttactt
      121 gagtactgat gaggtatgcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcatactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010824
VERSION     KHUT01010824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtcttg
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgatac tgctcgacta
      121 gagtatggaa gagggaaggg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg ccttcctggg ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010825
VERSION     KHUT01010825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           88 g           45 t
ORIGIN      
        1 tacgaagggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgca ggctggcctg
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaattg cttggcaaac tacaggtctt
      121 gagcccgaca gaggtcgtcg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgccaaa ggtgaagaca ggcgactggg tcggcgctga cgctcaggca cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010826
VERSION     KHUT01010826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           85 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcaatg
       61 taagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac tacatagcta
      121 gagtacagtt agggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctgtaactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010827
VERSION     KHUT01010827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacgtatggt gcaagcgttg ttcggattta ctgggcgtaa agagcgcgca ggcggattat
       61 taagtcgggt gtgaaatctc caggcttaac ctggaggctg catccgaaac tggtgatctg
      121 gagtcccgga gaggcagacg gaatacttgg tgtagcggtg aaatgcgtag atatcaagtg
      181 gaacaccgat ggcgaaggca gtctgctgga cggtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010828
VERSION     KHUT01010828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           75 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtggacggt
       61 taagtcagag gtgaaatcct gcagctcaac tgtagaattg cctttgatac tggccgtctt
      121 gaatcgatgt gaagtggtta gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaataccgat tgcgaaggca gatcactaac atcgcattga cactgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010829
VERSION     KHUT01010829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           39 c           90 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttagt
       61 taagttagat gtgaaatccc tgggcttaac ctgggaatgg catttaagac tgattagcta
      121 gagtttggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctggc ccgatactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010830
VERSION     KHUT01010830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttttg
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaattg catctgatac tgtatgacta
      121 gagtatgcta gaggaaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcaat ggcgaaggca actttctggg gcaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010831
VERSION     KHUT01010831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           81 g           53 t
ORIGIN      
        1 tacgtaaggg gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggtttta
       61 ttagtctgtg gttaaatccc ggggctcaac tccggaatcg tcacggaaac tgtaaaacta
      121 gaggtgtgta ggggcagatg gaattccaga agtaggggta aaatccgtag atctctggag
      181 gaacaccaaa cgcgaaggca ttctgctgga cactacctga cactgaggga cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01010832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010832
VERSION     KHUT01010832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           89 g           44 t
ORIGIN      
        1 tacgagtggc ccaagcgtta atcggaatca ctgggcttaa agcgtgcgca ggcggatttg
       61 aaggcgtctt gtgaaatccc acggctcaac cgtggaatcg cagggcgaac cacaagtctt
      121 gaggcaggta gggggagacg gaactgtcgg tggagcggtg aaatgcgtag atatcgacag
      181 gaacgccagt ggtgaagacg gtctcctggg cctgtcctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010833
VERSION     KHUT01010833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtttgc
       61 caagttgaga gtgaaatccc agggcttaac cctggaactg ctttcaaaac tggcagacta
      121 gagtatggta gaggatagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actgtctggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010834
VERSION     KHUT01010834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggcgatg
       61 atagtcagat gtgaaaggcc tgggcttaac ccgggaactg catttgatac tgcattgcta
      121 gagtccgaga gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctggc tcggtactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010835
VERSION     KHUT01010835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtaatc
       61 caagctgggt gtgaaagccc cgggctcaac ctgggaattg cattcagaac tggattgcta
      121 gagtacagaa gaggggtgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg acaccctggt ctgatactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010836
VERSION     KHUT01010836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           88 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggtaaag
       61 taagttaggt gtgaaagccc ggggctcaac ctcgggactg cacttaaaac tgctttacta
      121 gaatacagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtgattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010837
VERSION     KHUT01010837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           87 g           47 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgtg
       61 tgtgttaggt gtgaaagccc gaggcttaac ctcggaattg cgcctaaaac tacacggcta
      121 gagcattgga gagggtagcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01010838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010838
VERSION     KHUT01010838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcagtt gtgaaatccc agggctcaac cttggaactg caattgatac tggccgactc
      121 gagtcgaaga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaggcg gcttcctggc ttcagactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010839
VERSION     KHUT01010839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttgg
       61 taagtcgagt gtgaaatccc cgggctcaac ctgggcactg cattcgagac tgccaagcta
      121 gagtatggta gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg actacctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010840
VERSION     KHUT01010840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcctgg gtgaaatcct ccggcttaac cggagaagtg cctgagatac tggtcgtctg
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010841
VERSION     KHUT01010841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           89 g           48 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggccaga
       61 aaagtcggat gtgaaattcc ggggctcaac cccggagctg catttgaaac tttcaggcta
      121 gagtacagat ggggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctgtaactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010842
VERSION     KHUT01010842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c          101 g           34 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggccgtg
       61 cacgtggggt gtgaaagcgc tcggcttaac tgagcgaggc catcccatac ggcgcggctc
      121 gagccatgca gaggtccgcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaagcca gcggactggg catgggctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010843
VERSION     KHUT01010843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           95 g           42 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtcgaat gtgaaagccc agggctcaac cctggaagtg catccgaaac tatcaaacta
      121 gagtcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accgcctgga cggagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010844
VERSION     KHUT01010844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           96 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtgggc
       61 taagtcagct gtgaaagccc tgggctcaac ctgggaacgg cagttgatac tggtctacta
      121 gagtgtgata gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaat ggcgaaggca gccacctggg tcaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010845
VERSION     KHUT01010845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggatgca
       61 taagtcagtg gtgaaatccc cgagcttaac ttgggaactg ccgttgatac tatgtgtctt
      121 gaatgtcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcttactaca cgatgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010846
VERSION     KHUT01010846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttact
       61 taagtctggt gttaaagacg ggagctcaac tcccgcatgg cattggaaac tgggtgacta
      121 gagtatggta gaggtatcgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg cgatactggg ccataactga cgctgaggaa cgaaagccag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01010847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010847
VERSION     KHUT01010847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           82 g           58 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggattga
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg catatgatac tgttaatcta
      121 gagttcgaaa gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttactggt tcgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010848
VERSION     KHUT01010848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggtggctctc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggaggacta
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccactactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010849
VERSION     KHUT01010849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggctgttcga
       61 taagtctgct gtaaaagtca ggggctcaac ctctgaaagc cggtggatac tgtcggactt
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010850
VERSION     KHUT01010850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcgcgta ggcggccatg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaactg cgcctgatac tgtgtggctt
      121 gagggttgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt caacttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010851
VERSION     KHUT01010851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          104 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggagcgt
       61 taagtccggt ctgaaagccc ccggctcaac cggggaaggt ggctggaaac tggcgttctg
      121 gagggcgata gaggagcatg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gtgctctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010852
VERSION     KHUT01010852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           87 g           48 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggtggatcag
       61 caagtcagat gtgaaatctc agggctcaac cctgaaactg cgtctgaaac tgctagtcta
      121 gagtgttgga gggggtaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctacctgga caactactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010853
VERSION     KHUT01010853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttgat
       61 taagttagat gtgaaatccc tgggcttaac ctgggaatgg catttaagac tgattggctg
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga ctcatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010854
VERSION     KHUT01010854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           86 g           54 t
ORIGIN      
        1 gacagaggat gcaagcgttg ttcggaatta ctgggcgtaa agcgaacgta ggtggttttg
       61 tgtgtctggt gtgaaagccc ttggctcaac caaggaagtg cgccggaaac tgcaaaactg
      121 gagtgtagga gaggatggta gaattctcag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccggt ggcgaaggcg accatctgga ctataactga cactgatgtt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010855
VERSION     KHUT01010855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          108 g           39 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggctcac
       61 taagcagggc gtgaaagccc ccggcttaac cggggagggt cgtcctggac tggtgggctt
      121 gagggtggta ggggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccaaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010856
VERSION     KHUT01010856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccaag
       61 tcagtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tactcggctt
      121 gagtaccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010857
VERSION     KHUT01010857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggccttg
       61 caagttggat gtgaaagccc agggctcaac cttggaagtg cacccaaaac tgcttggctt
      121 gagtaccgga ggggctcaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cggtaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010858
VERSION     KHUT01010858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttata
       61 tgagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gatatgactg
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010859
VERSION     KHUT01010859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           96 g           50 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgtgta ggcggaacga
       61 tcagtcgggg gtgaaaggtt cgagctcaac tcggacattg ccttcgatac tgtcgttctt
      121 gaatgcagga gagggtagtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gctgcctggc ctgacattga cgctgagacg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010860
VERSION     KHUT01010860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           86 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaat
       61 taagtcagat gtgaaatccc aaggcttaac cttggaatgg catttgatac tggttagcta
      121 gagtacagaa gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctggt ctgttactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010861
VERSION     KHUT01010861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgagcgta gtcggttgtc
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaattg cattcgatac tgggcagcta
      121 gagttcggca gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg gcttcctgga ccagaactga cgatcaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010862
VERSION     KHUT01010862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           83 g           51 t
ORIGIN      
        1 tacgtagacc tcaagcgtta tccggattta ttgggcgtaa agcgcgtgta ggaggtttcg
       61 cgcgtctttg gttaaagacc actgcctaac agtggaagtg ccaaggatac ggcgaaacta
      121 gaggaagtta gaggtgcatg gagcgcacgg tgtaggggtg aaatccgttg atatcgtgcg
      181 aaacaccaaa ggcgaaggca atgcactggg actttcctga ctctgagacg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01010863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010863
VERSION     KHUT01010863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggtgtaa agggtgcgta ggcgggaagt
       61 caagtcggtg gtgaaatctt gccgctcaac ggtaagcgtg ccgccgatac tgatttcctt
      121 gagttcggaa gaggccggtg gaattcgtgg tgtagcggtg aaatgcatag agatcacgaa
      181 gaacaccagt ggcgaaggcg gccggctggt ccgaaactga cgctgaggca cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01010864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010864
VERSION     KHUT01010864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttgtt
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaattg cattcgatac tgggcaactc
      121 gagttcggaa gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg acttcctggt ccgtgactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010865
VERSION     KHUT01010865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           84 g           52 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatatg
       61 aaagtcagaa gtgaaatccc cgggctcaac ccgggaattg cttttgaaac tccatatcta
      121 gaatcccgga ggggccagta gaattcctag tgtagaggtg aaattcgcag atattaggag
      181 gaataccagt ggcgtaggcg actggctgga cgggtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010866
VERSION     KHUT01010866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccagt
       61 taagtcagcg gtgaaagttt tcggctcaac cggaaaaatg ccgttgatac tggctggctt
      121 gagtcacgga agggtacatg gaattggtgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgaaggca ttgtacttgc cgtgaactga cgctgaggca cgaaagcgcg
      241 ggtagcgaac agg
//
LOCUS       KHUT01010867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010867
VERSION     KHUT01010867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcctgc
       61 caagtctggt gtgaaagctc ggggctcaac cccgagtctg cattggaaac tggtaggctt
      121 gagtccggaa gaggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg agtctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010868
VERSION     KHUT01010868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           98 g           37 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgga
       61 agggttctgg gtgaaagccc ccagcttaac tggggaagtg cccggaatac cgcccgactc
      121 gaggggggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acacgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010869
VERSION     KHUT01010869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 tacggaggat gcgagcgttg ttcggaatca ctgggcttaa agcgcatgca ggcggaacgg
       61 aaggtgcttt gtgaaatccc tcggctcaac cggggaattg ctgggcaaac caccgttctt
      121 gaggcaggta gaggcgactg gaacctttgg tggagaggtg aaattcgtag atatcaaagg
      181 gaacgccggt ggcgaaagcg ggtcgctggg cctgtcctga cgctcagatg cgaaagcgta
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010870
VERSION     KHUT01010870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggcgtgc
       61 ttagtcttct gttaaaatcc tgggcttaac ccaggacttg cggaggaaac gggcatgctt
      121 gagggtgcga ggggccagtg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca actggctggc gcattcctga cactgagaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010871
VERSION     KHUT01010871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          105 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctggg
       61 taagtcgatg gtgaaatccc gtggctcaac cgcggggctg ccgtcgagac tgctcggctc
      121 gagcacggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacatcggt ggcgaaggcg gccagctggg ccgttgctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010872
VERSION     KHUT01010872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtttga
       61 caagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaaaac tgtcagactt
      121 gagggcggta gagggaggtg gaattccgtg tgtagtggtg aaatgcgtag atatacggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccgatcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010873
VERSION     KHUT01010873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           91 g           50 t
ORIGIN      
        1 tacgaaaggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggctatt
       61 taagtctgat gtgaaatccc cgggctcaac ctgggaattg catcggatac tgggtagcta
      121 gaatgcggta gaggggggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gccccctgga ctgacattga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010874
VERSION     KHUT01010874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           83 g           57 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggattta ttgggcgtaa agcgttcgta ggcggtttcg
       61 taagtcattt gtaaaatcct caggcttaac ttgggggctg caggtgatac tgcgaggctt
      121 gagagtggga gaggtaagcg gaatgcccgg tgtagcggta aaatgcgtta atatcgggta
      181 gaacaccaaa ggcgaaggca gcttactgga acatttctga cgctgatgaa cgaaagcgtg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01010875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010875
VERSION     KHUT01010875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcagag gtgaaatctt tcggctcaac cggaaaagtg cccctgatac tggccatctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010876
VERSION     KHUT01010876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttat
       61 taagtcggac gtgaaatccc tcggctcaac cgaggaactg cgtccgatac tgttaggctt
      121 gagtgtcgga gggggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccacctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010877
VERSION     KHUT01010877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggtcgat
       61 taagtcagat gtgaaagccc tgggctcaac ccgggaagtg catttgatac tggtcgactc
      121 gagtgcgaga gaggagatcg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaataccgat ggcgaaggca ggtctctggc tcgcaactga cactgagatt cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010878
VERSION     KHUT01010878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c           99 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccgg
       61 ccagtccggg gtgaaatcct tcggctcaac cgaagaatgg ccctggatac tgccggcctc
      121 gagggaggta ggggtcgacg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacgccggt ggcgaaagcg gtcgactgga cctctgctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010879
VERSION     KHUT01010879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggctaac
       61 taagtcaagt gtgaaatccc ccggctcaac cggggaactg cgcctgatac tggttggctt
      121 gagaacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtaactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01010880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010880
VERSION     KHUT01010880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccat
       61 caagtccgtg gtgaaatccc ccggcccaac cggggaagtg ctgcggatac tggtggtctc
      121 gagtggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU10999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010881
VERSION     KHUT01010881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU10999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg cttgacagac tggcggtctt
      121 gagccagcta ggggcgaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtcgctggg gctgcgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010882
VERSION     KHUT01010882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggaatta ctgggcgtaa agcgcgtgca ggcgggatgg
       61 gaagtctgat gtgaaagccc ggggctcaac cccggaagtg cattggaaac tcccgttctt
      121 gagtatcgga gaggaagggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaataccagt ggcgaaggcg cccttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010883
VERSION     KHUT01010883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 gacgaaggtg gcgagcgttg ttcggaatca ctgggcttaa agagtacgta ggcggcctgt
       61 taagcacttt gtgaaatccc tcggctcaac cgaggaattg ctgggtgtac tggcaggctt
      121 gaggcaagta ggggcgcatg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccgga ggcgaaagcg atgcgctggg cttgtcctga cgctgaggta cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010884
VERSION     KHUT01010884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatta ctgggcgtaa agggcgtgca ggcggccttg
       61 taagtggaat gtgaaatgcc ctggctcaac cagggaactg catcccagac tgcaaggctt
      121 gagtatagga gaggatgggg gaattcccgg tgtagcggtg aaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggc ctaatactga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010885
VERSION     KHUT01010885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           93 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcatt
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaacag cttctgatac tgagtgtcta
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg gcgtgctgga cctcctctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010886
VERSION     KHUT01010886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggagggcagg
       61 taagtcagtg gtgaaatccc cgagcttaac tcgggaactg ccgttgatac tatctgtctt
      121 gaatattgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttgctaca caactattga ctctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010887
VERSION     KHUT01010887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           85 g           55 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggcatat
       61 caagtctgat attaaagacc ggggcttaac ttcgggaatg tatcggaaac tgataagcta
      121 gaggacagta gaggaaaccg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg ggtttctggg ctgttactga cgctgaggta cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010888
VERSION     KHUT01010888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggaatca ttgggcttaa agcgtgtgca ggcggcggcg
       61 taagtgtcga gtgaaagcct gcggctcaac cgcagaacgg ctcggcatac tgcgtcgctt
      121 gaggtcggta gaggtgcttg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcctga ggcgaaagcg gagcactggg ccgattctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010889
VERSION     KHUT01010889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           82 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaaatg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tgcgtttctc
      121 gagggtagac ggagaaaaca gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggcgaaggcg gttttctagt ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01010890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010890
VERSION     KHUT01010890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 tacagggggc tcgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggcccgc
       61 caagtgcttt gtgaaatccc cgagctcaac tcgggaacag cagggcaaac tggcgggctt
      121 gaagcaggta ggggctggaa gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggggaagccg tccagctggg cctgtcttga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010891
VERSION     KHUT01010891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           85 g           49 t
ORIGIN      
        1 aacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggcggtcttt
       61 taagtttcgc gtgaaatccc tcggctcaac cgaggaacgg cgcggaaaac tgaaggactt
      121 gagtacggga gaagagggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacacctgt gacgaaagtg gctctctaga ccgatactga cgctcatacg cgacagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010892
VERSION     KHUT01010892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           88 g           54 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttttc
       61 taagtcacgt gtgaaatctt ccggctcaac cggaaaactg cgcgtgatac tggataactt
      121 gagtgaggta ggggtgtgtg gaacttccgg tggagcggtg aaatgtgtag agatcggaag
      181 gaacgccagc agcgaaagcg gcacactggg ccttttctga cgctgaggca cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01010893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010893
VERSION     KHUT01010893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgagcgcg ggtggtctct
       61 taagtcagaa gtgaaagccc tgggctcaac ccaggaagtg cttttgatac tgggagactt
      121 gaatgtggga gagggtgcta gaatacctag tgtagtggtg aaatacgtag atattaggtg
      181 gaacaccggt ggggaagccg ggtacctggc ccaacattga cacttaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01010894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010894
VERSION     KHUT01010894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           87 g           52 t
ORIGIN      
        1 tacgaaggcc ccaagcgtta tccggaatta ttgggcgtaa agggtccgta ggcggtatgt
       61 atagtcacag gttaaagctc ccggctcaac tgggaatatg cttgtgaaac gtgcgtacta
      121 gaggatgtga gaggcatgtg gaactcacgg tgtagcggtg aaatgcgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca acatgctggc gcattcctga cgctgaggga cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010895
VERSION     KHUT01010895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtcttt
       61 taagctggat gtgaaagccc cgggcttaac ctgggaactg cattcaggac tgggagactc
      121 gagtctggga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg accgcctggc ctaagactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010896
VERSION     KHUT01010896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgtttga
       61 taagttatct gtgaaatacc tgggcttaac ctgggagggt cagataagac tgttgaacta
      121 gagtatagga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctgcctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010897             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010897
VERSION     KHUT01010897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           93 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcggat gtgaaacctc caggctcaac ctggagtcgc catccgatac tgctgtggct
      121 tgagtccggt aggggagcat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc ggtgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010898
VERSION     KHUT01010898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           88 g           51 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcaaat
       61 caagttaggc gtgaaatccc tgggcttaac ccaggaactg cgtttaatac tggttagcta
      121 gagattggta gaggaaagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccaga ggcgaaggcg actttctggg ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010899             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010899
VERSION     KHUT01010899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           85 g           54 t
ORIGIN      
        1 tacgagtgcc ccaagcgttg tccggaatta ttgggcgtaa agggtgtgta ggcggcatgg
       61 ttagtctttt gttaaagctc ccggctcaac cgggaaggtg caaaggaaac ggccattgct
      121 agaggatgtg agaggtgaat ggaactcatg gtgtaggggt gaaatccgtt gatatcatgg
      181 ggaacaccaa aagcgaaggc aattcactgg cgcattcctg acgctgaaac acgaaagcgt
      241 gggtagcgaa tggg
//
LOCUS       KHUT01010900             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010900
VERSION     KHUT01010900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          102 g           41 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggcttgg
       61 gaagtctatg ggtgaaaggc cggagctcaa ctccgcgtag gcccaggata ctctcaggct
      121 ggagacacgt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010901             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010901
VERSION     KHUT01010901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcggtttct
       61 taagtttggg gtgaaaggtt cagcgctcaa cgcgaacact gccttgaata ctgggagact
      121 tgagttcggg agggggtagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctacctgg cctagaactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010902             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010902
VERSION     KHUT01010902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcctga
       61 aaggtcggtt gtgaaaactg gatggctcaa cctccagacg tcgatcgaaa ccatcaggct
      121 agagtccgga agaggagaat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 agaacacccg tggcgaaggc ggttctctgg gacggtactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010903             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010903
VERSION     KHUT01010903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c          101 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtga ctgggcgtaa agcgcccgca ggcggtgcgc
       61 cacgtttgcg ctgacagctc ctggcttaac taggagagcg gtgcgaagac gggcgtactg
      121 agaggaccag agagggacat ggaactccgg gtgtagtggt gaaatgcgta gatatccgga
      181 agaacaccaa aggcgaaggc agtgtcctgg ctggcacctg acgctgaggg gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010904             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010904
VERSION     KHUT01010904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           82 g           56 t
ORIGIN      
        1 tacgtagggt tcaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtgtatt gcgaaatcct gtggctcaac catatggact gtaatgcata ctggcaaact
      121 agaggacatg agaggcaagt ggaattactg gtgtagcagt gaaatgcgta gagatcagta
      181 ggaacaccaa tggcgaaggc agcttgctgg catgtatctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010905             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010905
VERSION     KHUT01010905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggattgg
       61 cgcatcgatt gctgaaagcc cccggctcaa ccggggaacg ggcgtcgaga tggccagtct
      121 cgaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accccttctg acgctgagac gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010906             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010906
VERSION     KHUT01010906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcggat gtgaaatctc cacgctcaac gtggaggcgc cattcgatac tgccgtgact
      121 tgagtacggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010907             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010907
VERSION     KHUT01010907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           52 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtgagaa ctgaaatctc cagagctcaa ctcggaaact ggttttcata ctggcagact
      121 ggaggaacgc agaggtgact ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaactccaa tggcgaaggc aggtcactgg gcgttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01010908             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010908
VERSION     KHUT01010908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           52 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatta ttgggcgtaa agcgcatgta ggcggcttga
       61 tcagtctggc gtgaaagccc tgagcttaac tcaggaagtg cgctggaaac agtcaagctt
      121 gagtaccgga ggggaaggtg gaattctcga gtgtagaggt gaaattcgta gatattcgga
      181 agaacatcag tggcgaaggc ggccttctgg acggatactg acgctgagat gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010909             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010909
VERSION     KHUT01010909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           32 c           65 g           78 t
ORIGIN      
        1 tacggggggg actaacgtta ttcatcataa ctgggcgtaa agggtacgta ggtggctttt
       61 taagtcttaa tcaaagaatc taagcttaac ttagaaattg tttttgatac tgttaagctt
      121 gaggtcaata gaggttagtt atattcttga agtaaaagta aaatctttag atatcaagag
      181 gataaccaag tggttaatgc gactttctgg attgatgctg acgctgaggt acgaaggcat
      241 gggtatcaaa cggg
//
LOCUS       KHUT01010910             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010910
VERSION     KHUT01010910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           93 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcggcggca
       61 taagttcgtg gtgaaatccc atcggctcaa ccgtggaatc gcttcgaata ctgtgtcgct
      121 agagtgggat aggggaccgc agaactgccg gtggagcggt gaaatgtgtt gatatcggcg
      181 ggaatgccag tgccgaaagg ggcggtctgg accctcactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010911             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010911
VERSION     KHUT01010911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c          103 g           46 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggattta ttgggcgtaa agggctcgta ggcggacagg
       61 taagtctggc gtgaaatctc caccactcaa cggggagggg ccgtcggata ctgcttgtct
      121 ggagtgcggt agaggaccat ggaattccag gtgtagcggt ggaatgcgta gagatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgtaactg acgctgagga gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010912             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010912
VERSION     KHUT01010912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           64 c          102 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtcgg
       61 cgcgtcggtc gctgaaatcc cccggctcaa ccggggaagg ggcgccgaaa cggccggcct
      121 ggagggacgt agggggggct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagcccctgg acgtctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010913
VERSION     KHUT01010913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           87 g           50 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggcaatg
       61 taagtctgct gttaaagagt ctagctcaac tagataaagg cggtggaaac tacatagcta
      121 gagtacgttc ggggcagaag gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaagcg ttctgctagg ccgtaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01010914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010914
VERSION     KHUT01010914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 gacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggcggctcgg
       61 taagtctgtt gtgaaagcct tgggcttaac ccagggaggt caatggaaac taccgggctt
      121 gaatgtggga ggggtgactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaat ggcgaaagca ggtcactgga ccactattga cgctgatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01010915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010915
VERSION     KHUT01010915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtctgta ggtggtgctg
       61 taagtctgct gttaaagagt cacgctcaac gtgataagag cagtggaaac tacaggacta
      121 gagtgcgttc ggggcagaag gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaagcg ttctgctagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01010916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010916
VERSION     KHUT01010916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           80 g           61 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattta ctgggtgtaa agggtgtgta gacggtctgt
       61 taagttcgtt gttaaatttt tcggcctaac tgaaaacttg cgatgaaaac tggcggactt
      121 gagggtgaaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaagca gcttcttggt tcacatctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010917
VERSION     KHUT01010917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           91 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtcttt
       61 taagtctgat gtgaaagccc ccggcttaac cggggagggt cattggaaac tgggagactt
      121 gagtgcagaa gaggaaagcg gaattccatg tgtagcggtg aaatgcgtag atatatggag
      181 gaacaccagt ggcgaaggcg gctttctggt ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010918
VERSION     KHUT01010918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           91 g           43 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggcggctcgg
       61 caagtccgtt gtgaaagctc ccggcttaac tgggagaggc caatggatac tgccgggctt
      121 gaatcgggga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaagca ggtgactgga cccgtattga cgctcagcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01010919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010919
VERSION     KHUT01010919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           94 g           42 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggccggg
       61 taagttggat gtgaaatctc ctggctcaac tgggagagga cgtacaagac tgcccggctc
      121 gaggacggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccgtgcctga cgctgagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010920
VERSION     KHUT01010920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacagagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggcctgt
       61 taagtcgaac gtgaaatccc tgggctcaac ccaggaactg cgtccgagac tgataggctt
      121 gaatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtattga cactgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010921
VERSION     KHUT01010921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           63 c          105 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 cgcgtccggg gtgaaatcct cccgctcaac gggagaacgg cctcggatac gggcgggctg
      121 gaggcgggta ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcgtgctgga cccgatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01010922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010922
VERSION     KHUT01010922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           93 g           44 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttact
       61 taagtcgaat gtgaaagccc ttggctcaac cgaggaagtg catccgaaac tgggtagcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010923
VERSION     KHUT01010923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           98 g           41 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgcac
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaacgg cgcttgagac tggtgcgctc
      121 gagttcggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgat ggcgaaggca gccgtctgga ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010924
VERSION     KHUT01010924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           83 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatcat
       61 taagtaggaa gtgaaaggtt ggagctcaac tccgacactg ctccctatac tggtggtctt
      121 gagtaccgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cggaaactga cgctgagata cgaaagctaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01010925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010925
VERSION     KHUT01010925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           80 g           56 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttttg
       61 ttagtctttt gttaaagctc tcggcttaac cgggaacatg cagaggaaac ggcaaaacta
      121 gaggatgcga gaggtgaatg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01010926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010926
VERSION     KHUT01010926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacgaaggtg gctagcgttg ttcggattta ttgggcgtaa agggtccgca ggcggtttag
       61 aaagtcaggt gtgaaatcca acagctcaac tgttgacacg catttgaaac ttctagacta
      121 gagtgtgaga ggggtgaatg gaatttccag tgtaggggtg aaatccgtag atattggaaa
      181 gaacaccggc cgcgaaggcg attcactggc tcattactga cgctcaggga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010927             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010927
VERSION     KHUT01010927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctctg
       61 ataagtgagg tgtgaaagcc ctgggctcaa cccaggaagt gcacttcaaa ctatcgagct
      121 tgaatgctgg agaggaaagc ggaattctcg gtgtagaggt gaaattcgta gatatcgaga
      181 ggaacacccg tggcgaaggc ggctttctgg acagcgattg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010928
VERSION     KHUT01010928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           93 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcatga
       61 caagtctggg gtgaaatcct ccggctcaac cggagaattg cctgggaaac tagcatgctc
      121 gagggaggta gaggtggccg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga gccgaaaggg ggccactgga cctcttctga cgctgatgcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010929
VERSION     KHUT01010929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           90 g           52 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggtttgg
       61 taagttggat gtgaaatctc ctggctcaac caggaggtgt cgttcaaaac tgccagactc
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccgttcctga cgctaagaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01010930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010930
VERSION     KHUT01010930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          109 g           36 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggattgt
       61 cgcatcgtcg gtgaaagccc cccgctcaac gggggagggt ccggcgagat gggcagtctg
      121 gaggcaggga gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctcgctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010931
VERSION     KHUT01010931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           97 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtcgat
       61 caagtccggg gtgaaatcgt tcggcttaac cggacaagtg ccttggatac tggttggctg
      121 gagggaggta ggggcagctg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010932
VERSION     KHUT01010932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           96 g           48 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggctaac
       61 atagtcaggc gtgaaagccc cgggcttaac ctgggaactg cgtttgatac tggttagcta
      121 gaggacgaga gaggaaagtg gaataccgag tgtagaggtg aaattcgtag atattcggtg
      181 gaacatcaga ggcgaaggcg gctttctggc tcgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010933
VERSION     KHUT01010933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           85 g           56 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ctgggcgtaa agagtgcgta gacggcttgt
       61 taagtttgtt gttaaaggct tcagcctaac tgaaggattg caatgaaaac tggcaggcta
      121 gagggtgaga gagagaagtg gaatttctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcatttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010934
VERSION     KHUT01010934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           76 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggctga
       61 taagtcagcg gtgaaatctc cgggcttaac ctggaaactg ccattgatac tatcagtctt
      121 gaatctcgcg taggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttacttca cgagtattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010935
VERSION     KHUT01010935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctcg
       61 taagttggat gtgaaagccc ttggcttaac caaggaagtg cattcaaaac tgcttgactt
      121 gagtaccgga gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010936
VERSION     KHUT01010936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           97 g           41 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtccgc
       61 tgtgtcccgt gtgaaagccc cgggctcaac ctgggaattg catgggaaac tagcgggctt
      121 gagtgcggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010937
VERSION     KHUT01010937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           73 g           60 t
ORIGIN      
        1 gacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggataaa
       61 taagtcagag gtgaaagcct gcagctcaac tgtagaattg cctttgaaac tgtttatcta
      121 gagtacactt gaggtggctg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca agtcactaag atgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010938             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010938
VERSION     KHUT01010938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           81 g           61 t
ORIGIN      
        1 tacgagaggt acaagcgttg cccggattta ctgggcgtaa agcgtttcgt aggcgcatat
       61 aaaagttgtg tttcaaagtc cggggcttaa cctcgggaag ggacgcaata ctgtatatgt
      121 tgaattattt tggggctact ggaactatcg gtgtaggggt gaaatccgtt gatatcgata
      181 ggaactccaa gggcgaaggc aggtagctaa gaatatattg acgctgagga acgacagcta
      241 gggtagcgaa aggg
//
LOCUS       KHUT01010939             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010939
VERSION     KHUT01010939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           97 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttgca
       61 caagcttgat gtgaaagccc cgggcttaac ctgggaatgg cattgaggac tgtgtggctt
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccgacactga cgctgagggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010940             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010940
VERSION     KHUT01010940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           94 g           55 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtccgta ggcggttgtg
       61 taagtctggt gtgaaatctt cgccgcttaa cgggaagcag ccactggaaa ctgcataact
      121 tgagtatggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatctg tggcgaaggc ggcttgctgg gccattactg acgctgaggg acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010941             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010941
VERSION     KHUT01010941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           83 g           52 t
ORIGIN      
        1 tacgagagcc tcaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggacatg
       61 ttagtcttgt gtgaaagcct gttgctcaac aacagatccg cataggaaac ggcatgactg
      121 cgagggtgcg aggggtgaat ggaactcatg gtgtaggggt gaaatccgtt gatatcatgg
      181 ggaacgccaa aagcgaaggc aattcactgg cgcatacctg acactgaaac acgaaagcca
      241 gggtagcgaa tggg
//
LOCUS       KHUT01010942             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010942
VERSION     KHUT01010942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           88 a           36 c           67 g           63 t
ORIGIN      
        1 cacaggggat gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta gattgtttaa
       61 taagtctttt gttaaatctc ttggcacaac caaaaaaaag cgaaagatac tgtttaacta
      121 tgagtataaa tagagcaaag ggaatttcca gtgaagcggt gaaatgtgta gatattggaa
      181 ggaacaccgg tagcgaaagc gctttgctag tttataactg acattgagga acgaaagcta
      241 agggagcaaa cggg
//
LOCUS       KHUT01010943             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010943
VERSION     KHUT01010943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcgggt gtgaaaactc caggctcaac ctggagatgc cacccgatac tgctatggct
      121 tgagttcggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccgacactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010944             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010944
VERSION     KHUT01010944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          103 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctctt
       61 taagtctcgt ggtcaaatgc cggggctcaa ccccgagcgt gccggggata ctggagggct
      121 ggagacaggt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg acctggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010945             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010945
VERSION     KHUT01010945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggcttct
       61 taagtctgag gtgaaaggtt caacgctcaa cgtgaacagt gccttggata ctgggaggct
      121 tgagtatggg agagggcagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccca tggcgaaggc agctgcctgg cctaatactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010946             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010946
VERSION     KHUT01010946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 ttagtcgggt gtgaaatctc cacgcttaac gtggaggggc cactcgatac tgccgtgact
      121 cgagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcactctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010947             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010947
VERSION     KHUT01010947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggttcgt
       61 acagtcaggt gtgaaattcc cgggctcaac ctgggggctg cacttgatac gtatggactt
      121 gagtgaggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccttgtactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010948             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010948
VERSION     KHUT01010948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           93 g           46 t
ORIGIN      
        1 cacgtaggga ccaagcgttg tccggattta ttgggcgtaa agcgctcgta ggcggtttgg
       61 taagtcgggt gtgaaacctc caggctcaac ctggtgacgc cacccgatac tgccatgact
      121 tgagttcggt aggggagcgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccgctctgg gccgaaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010949             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010949
VERSION     KHUT01010949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           88 g           57 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggtggtcagg
       61 taagtctgat gtgaaatcat catcgcttaa cgagatgtcc gcatcggata ctgtctgact
      121 tgagtatagg agaggataac ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 ggaacacctg tggcgaaggc ggttatctgg cctaatactg acactgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010950             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010950
VERSION     KHUT01010950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctatt
       61 taagtctgtg gtgaaaggtt caacgctcaa cgtgaacagt gccgtggata ctggatagct
      121 tgagttcggg agaggagagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaaccccgg tggcgaaggc ggctctctgg cctagaactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010951             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010951
VERSION     KHUT01010951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           99 g           45 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggaccgg
       61 tgtgtctggc gtgaaatctc cactgcttaa cagggagggg ccgctggaaa ctgccgatct
      121 tgagtatggt agaggaccat ggaattccgg gtgtagcggt ggaatgcgta gatatccgga
      181 agaacaccgg tggcgaaggc ggtggtctgg accaaaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010952             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010952
VERSION     KHUT01010952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           64 c          101 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggcagg
       61 cacgtcggtc gctgaaagcc cccggctcaa ccggggaagg ggcgccgata cgaccggcct
      121 ggaggggcgt aggggggact ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagtccctgg acgccatctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010953             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010953
VERSION     KHUT01010953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          109 g           35 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattca ttgggcgtaa agggcgcgta ggcggctctc
       61 agcagtggcg cgtggaatct cccggctcac ctgggagggg ccgtgccaga cgggggagct
      121 ggaggcaggt agagggtcgc ggaacgggcg gagtaggggt gaaatccgta gagaccgtcc
      181 ggaacaccga tggcgaaggc agcgacctgg gcctgtcctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cgga
//
LOCUS       KHUT01010954             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010954
VERSION     KHUT01010954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          103 g           41 t
ORIGIN      
        1 gacggagggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggaccgt
       61 taagtctggc gtgaaatctc caccgctcac cggggagagg tcgctggaaa ctggcagtct
      121 ggagttcggc agagggtcat ggaattccgc atgtagcggt ggaatgcgta gatatgcgga
      181 agaacaccgg tggcgaaggc ggtggcctgg gccgaaactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010955             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010955
VERSION     KHUT01010955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgag
       61 gaagtcggat tgtgaaagcc cggggctcaa ccccggaggt gcatccgaga ctctttggct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggacctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010956             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010956
VERSION     KHUT01010956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           48 t
ORIGIN      
        1 tacagagggg gctagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggctgtccgt
       61 taagtctgat gtgaaagccc tgggctcaac tcaggaattg cattggaaac tggcggactc
      121 agagtaccgg agagggaagc ggaattcctg gtgtagcggt gaaatgcgta gataccagga
      181 ggaacaccgg tggcgaaagc ggtttcctgg ccgagtactg acgctgaggc acgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010957
VERSION     KHUT01010957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggccaag
       61 caagtcggat gtgaaagccc agggctcaac cctggaagtg cactcgatac tgcatggctc
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010958             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010958
VERSION     KHUT01010958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           85 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtgggtgct
       61 tacagtcgaa tgtgaaaacc ccgagctcaa cttgggaact gcatgcgata ctgaacacct
      121 agagtatagt agagggaagt ggaatttccg gagtagcggt gaaatgcgta gatatcggaa
      181 ggaacaccag tggcgaaggc ggcttcctgg gctaatactg acactgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010959             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010959
VERSION     KHUT01010959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           98 g           43 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggattta ttgggcgtaa agcgcgcgca ggcggtcacg
       61 ggcagtggtg cgtggaatct cccggctcac ctgggagggg ccgcatcata cgacgtgact
      121 tgaggcaggt agaggatcgc agaacgggcg gagtaggggt gaaatccgta gagaccgtcc
      181 ggaataccag tggcgaaggc ggcgatctgg acctgttctg acgctcgggc gcgaaagcgt
      241 gggtagcgaa cgga
//
LOCUS       KHUT01010960             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010960
VERSION     KHUT01010960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           61 c           95 g           39 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcgggtcgt
       61 taagtgtccg cgtgaaatcc cacggctcaa ccgtggaact gccgggcata ctggcgacct
      121 tgaggcactc aggggcgagc ggaacaggcg gtggagcggt gaaatgcgta gatatcgcct
      181 ggaacgccaa tggtgaaaac agctcgctgg gggtgtcctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010961             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010961
VERSION     KHUT01010961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           48 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ttgggcgtaa agcgcatgta ggcggtgcga
       61 tcagtcggat gttaaagacc cacggctcac ccgggggaat gcgtccgata ctgtcgcgct
      121 agagtgtgga agaggagagt ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccgg tggcgaaggc ggctctctgg tccacaactg acgctgagat gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010962             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010962
VERSION     KHUT01010962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtcct
       61 gtaagtcgga agtgaaattt cacggctcaa ccgtgaagct gcttctgata ctgcggatct
      121 ggagatcggt agaggtcggt agaattacag gtgtagcggt ggaatgcgta gatatctgta
      181 agaatacccg tggcgaaggc ggccgactgg gccgaatctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010963             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010963
VERSION     KHUT01010963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           93 g           51 t
ORIGIN      
        1 tacggaaggt gcaagcgttg tccggattga ctgggtgtaa agggcgtgca ggcggaggaa
       61 tatgtcggga gtgaaatcgt gcggcttaac cgtatcaatg gcttccgaaa ctattcccct
      121 tgagtgcgag agaggcagat agaattcctg gtgtagcggt ggaatgcgta gatatcagga
      181 agaataccgg aggcgaaggc ggtctgctgg ctcgtaactg acgctgaggc gcgaaagtgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010964             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010964
VERSION     KHUT01010964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          100 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtcgtt
       61 taagtcttac tgtgaaatcc cggggctcaa ccccggggct gcagaggata ctggacgact
      121 ggagacaagt agaggcgagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccag aggcgaaggc ggctcgctgg gcttggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010965             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010965
VERSION     KHUT01010965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           83 g           55 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtctgta ggcggctttt
       61 caagtcaggt gcgaaatctt gcggctcaac cgtatagact gtgcttgaaa ctgaatcgct
      121 agaggtaggt agaggcaagt ggaatttctg gtgtaggagt gacatccgta gatatcagaa
      181 ggaacaccaa tggcgaaggc agcttgctgg gccttacctg acgctcagag acgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010966             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010966
VERSION     KHUT01010966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          103 g           42 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgg
       61 gaagtcggct tgtgaaagcc cggggctcaa ccccggaggt gcggccgata ctccctggct
      121 ggaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggacctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010967             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010967
VERSION     KHUT01010967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 tgagtcggat gtgaaatctc caggcttaac ctggagaggc cattcgatac tgccgtggct
      121 agagtccggt agaggagcat ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggtgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010968             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010968
VERSION     KHUT01010968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           51 t
ORIGIN      
        1 gacagaggtg gctagcgttg ttcggattca ttgggtgtaa agggtgcgta ggcggtttgg
       61 aaagtctgat gtgaaatccc acagctcaac tgtggacatg catttgaaac ttccagacta
      121 gagtgcgaga gaggtgaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt agcgaaggcg cttcactggc tcgcaactga cgctgagtgc acgaaggcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010969             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010969
VERSION     KHUT01010969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           77 g           55 t
ORIGIN      
        1 tacgtaggac ccaagcgtta tccggagtga ctgggcgtaa agagttgcgt aggcggtctg
       61 taaagcgaat agtgaaatat gatggctcaa ccatcagcac attattcgaa ctcacagact
      121 cgagaatggt agaggtgatt ggaatttcta gtgtaggggt aatatccgta gatattagaa
      181 ggaacaccaa tggcgtaggc agatcactgg accatttctg acgctgaggc acgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01010970             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010970
VERSION     KHUT01010970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaactgt gcgagcgtta ttcggagtca ctgggcttaa agcgcacgta ggcggcatcg
       61 taagttcggg gtgaaatcct gtcggctcaa ccgcggaatt gccctgaaga ctgcgaagct
      121 cgaggaaggt aggggtgtgt ggaacttccg gtggagcggt gaaatgtgtt gatatcggaa
      181 ggaacgccgg tggcgaaagc gacacactgg gccttatctg acgctgaggt gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010971             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010971
VERSION     KHUT01010971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           50 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcctag
       61 taagtcgggt gtgaaaactc caagctcaac ttggagcggc cactcgatac tgctatggct
      121 agagtctggt aggggatcac ggaattcctg gtgtagcggt gaaatgctta gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccagtactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010972             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010972
VERSION     KHUT01010972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           66 c          102 g           31 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgcgta ggcgggccgg
       61 cacgtcggat gctgaaagcc cccggctcaa ccggggaagg ggcaccgata cgaccggcct
      121 ggagtggggt agggggagcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggctcctgg accccgactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010973             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010973
VERSION     KHUT01010973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctcc
       61 gcaagtcagt tgtgaaatct ccgagcttaa ctcggaaagg tcaactgata ctgcggggct
      121 agagtgcgga aggggcaact ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010974             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010974
VERSION     KHUT01010974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           85 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggaccat
       61 gtaagtcagg ggtgaaatcc cggggctcaa ccccggaact gcctttgata ctactggtct
      121 tgaatgttgt tgaggtgggc ggaacgggtc atgtagcggt gaaatgcata gatatgaccc
      181 ggaacaccga ttgcgaaggc agcccgctaa gcaaccattg acgctgaggc acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01010975             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010975
VERSION     KHUT01010975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcgggg
       61 ttagttggat gtgaaacgcc ttggctcaac caaggcactg cattcaatac tgccctgctc
      121 gagcactagc gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacaccagt ggcgaaggcg agtctctaga tagtatgctg acgctgaggc acgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010976             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010976
VERSION     KHUT01010976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           86 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtgtgta ggcggcgcgt
       61 taagtgcagc gcgaaatctt gcggctcaac cgtatagact gtgttgcata ctggcgtgct
      121 tgaggacgtg agaggcaagt ggaatttgta gtgtagcagt gaaatgcgta gatattacaa
      181 ggaacaccaa tggcgaaggc agcttgctgg cacgtatctg acgctgagac acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01010977             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010977
VERSION     KHUT01010977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           64 c          113 g           32 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ctgggcgtaa agggcgcgca ggcggccgcg
       61 tcagcccgtc gcgaaagccc ccggctcaac tgggggaggt cggcggggac ggcgcggctt
      121 agaggccggt aggggggcac ggaattcccg gtgtagtggt gaaatgcgta gagatcggga
      181 ggaacaccag tggcgaaggc ggtgccctgg gccgggcctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01010978             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010978
VERSION     KHUT01010978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           79 g           58 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtacgta ggcgggtatt
       61 taagtgcaat gtgaaatctt gtggctcaac catatagact atgttgcata ctggatacct
      121 agaggatggc agaggcaagt ggaatttcta gtgtagcagt gatatgcgta gatattagaa
      181 ggaacaccaa tggcgaaggc agcttgctgg gccacccctg acgctgaggt acgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01010979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010979
VERSION     KHUT01010979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agcggaagca ggtggtcttg
       61 tttgtctggg gtgaaatccc cgagcttaac tcgggagtgt cctcagaaac ggcgagactg
      121 gagtcctggc gagggttgta gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccaga agcgaaggcg gcaacctgga caggtactga cactcaattc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010980
VERSION     KHUT01010980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           82 g           53 t
ORIGIN      
        1 tacgagggga gcaagcgtta ttcggaatta ctgggcgtaa agggtgtgta ggcggtatat
       61 taagtcagtt gttaaattcc tcggcccaac tgaggatcag cgacagatac tgatgtacta
      121 gagcgcagaa gagagaagtg gaattatcgg agtagcggta aaatgcgtag atctcgatag
      181 gaacaccgat ggcgaaggca gcttcttggt ctgatgctga cgctgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010981             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010981
VERSION     KHUT01010981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           96 g           50 t
ORIGIN      
        1 tacggaggga gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggaaat
       61 taagtgagaa gtgaaaggct ggggcttaac cccatgcagt gctcctcata ctggttttct
      121 tgagggtggt agagagaagt ggaatggctg gtgtagcggt gaaatgcgta gatatcagtc
      181 ggaacaccag tggcgaaggc ggcttcttgg gccattactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01010982             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010982
VERSION     KHUT01010982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          105 g           39 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggacggt
       61 gcaagtcagg ggtgaaattc tggggctcaa ccccggagct gctcttgata ctgcctgtct
      121 agggaccggt aggggccggt ggaattcccg gtgtagcggt ggaatgcgta gagatcggga
      181 agaacacccg tggcgaaggc ggccggctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010983
VERSION     KHUT01010983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccag
       61 caagtcgggg gtgaaatccc acggcctaac cgtggaagtg cctccgatac tgcggggctc
      121 gagggaggta ggggcgggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acccgctgga tctctgctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010984
VERSION     KHUT01010984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           58 c          113 g           37 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggattca ctgggcgtaa agggcgcgta ggcggccgca
       61 tcagtcgggc ctgtaagtgc ggggctcaac cccgtgatgg gctcgggacg gtgtagctgg
      121 ggcgcggcag agggagacgg aatgccgggt gtagtggtga aatgcgtaga gatccggcgg
      181 aacaccggtg gtgaagacgg tctcctgggc cgagagccga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01010985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010985
VERSION     KHUT01010985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           83 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgtgta ggcggtgtgc
       61 taagtcagat gtgaaagccc gaagctcaac ctcggaactg catccgatac tggcacacta
      121 gagaattgaa gaggagtcta gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaataccaat tgcgaaggca ggactctggg caaattctga cgctgaggca cgaaggctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01010986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010986
VERSION     KHUT01010986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           60 c           96 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcggag gtgaaagcct gcagctcaac tgcagaagtg cccccgatac tgatcgcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01010987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010987
VERSION     KHUT01010987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           81 g           52 t
ORIGIN      
        1 tacgaaggcc ccgagcgtta tccggattta ttgggcgtaa agcgttaata ggcggttctg
       61 taagtattcg gtcaaatctc atggctcaac catgaggctg ccgggtaaac tgcaggactt
      121 gaggtcggaa gaggcaagcg gaactaccgg tgtagtggta aaatgcgttc atatcggtag
      181 gaacaccaaa ggcgaaggca gcttgctggt acgaacctga cgctaaataa cgaaagcgtg
      241 gggagcgaat ggg
//
LOCUS       KHUT01010988             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010988
VERSION     KHUT01010988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcggat gtgaaaactc agggctcaac cctgagacgc catccgatac tgctgtgact
      121 tgagtctggt aggggagaac ggaattccag gtgtagcggt gaaatgcgca gatatctgga
      181 ggaacaccga tggcgaaggc agttctctgg gccagtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01010989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010989
VERSION     KHUT01010989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           37 c           91 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttatct gtgaaatagc tgggcttaac ctgggttggt cagatgatac tgttaggcta
      121 gagtatagta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctgtctgga ctaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01010990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010990
VERSION     KHUT01010990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcttg
       61 taagtcagga gtgaaagccc tcggcttaac cggggaagtg cttttgaaac tgcaagactt
      121 gaatactgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010991
VERSION     KHUT01010991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgagtgta ggtggccgcg
       61 taagtcggtt gtgaaatccc tgggctcaac ctgggaattg cagccgatac tgcgtggctc
      121 gagttcggta gagggcagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccgat ggcgaaggca actgcctggg cctgaactga cactgtgact cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01010992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010992
VERSION     KHUT01010992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggaagg
       61 caagtcggac gtgaaaggct tcggctcaac cggagacggc cgttcgaaac tgcctttctt
      121 gagggtcaaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt tgacatctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01010993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010993
VERSION     KHUT01010993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggtttaa
       61 aaagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaaaac tagtagactt
      121 gagtcggaca gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gctgcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010994
VERSION     KHUT01010994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           75 g           57 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggtttgg
       61 taagtcaggg cttaaaactc gccgcttaac ggcgaatctg gctttgatac tgccgaactt
      121 gagtatggaa gaggcaagcg gaattctcgg tgtagtagta ataagcgttg atatcgagaa
      181 gaacaccaaa tgcgaaggca gcttgctggt acattactga cactcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010995
VERSION     KHUT01010995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agggcgtgta ggtggttagg
       61 aaagttggct gtgaaagctc ctggctcaac tgggagaggt cagtcaaaac ttcctgactt
      121 gagggtcgga gaggggtgtg gaattcccgg tgtagcggtg gaatgcttag agatcgggag
      181 gaacaccagt ggcgaaggcg acaccctggc cgacaactga cactgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010996
VERSION     KHUT01010996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           84 g           52 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatga ttgggcgtac agggtgcgca ggcggtatat
       61 taagtcaact gtcaaatccc ccagcttaac tggggaccgg cggtagatac tggtatgcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01010997             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010997
VERSION     KHUT01010997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           98 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggagtgta ggcggttggc
       61 caagtcggat gtgaaagctt caccactcaa cgggaagagg tcatccgaaa ctggtcggct
      121 agagtagggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatcgg tggcgaaggc ggcttgctgg gcccttactg acgctgagac tcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01010998             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010998
VERSION     KHUT01010998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           85 g           52 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtcgga
       61 taagttaaca atggcaaatc agggctcaac cctgtggaag ttggtaatac tgtctgactg
      121 gaggacggga gaggttaacg gaactccctg tggagcggtg aaatgcgtag atatagggaa
      181 gaacaccaaa aagcgaaggc agttaactat tctgttcctg acattgaggc acgaaagcgt
      241 ggggagcgaa aagg
//
LOCUS       KHUT01010999             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01010999
VERSION     KHUT01010999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           95 a           28 c           66 g           65 t
ORIGIN      
        1 tacgggagga gcgagcatta ttcggaatga ttaggcgtaa agggttcgta ggtggttttt
       61 taagttgaaa aaaacagaat aaagctcaac tttataaatt ttttcaaaac tgaaagactt
      121 gagtataaat agaggataat ggaatttcta ttggagggat aaaatacgtt gataatagaa
      181 ggaagaccca aagcgaaggc aattatctgg gtatatactg acactgagga acgaaagctt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01011000             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011000
VERSION     KHUT01011000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggcgtaa agggcgcgta ggcggtctgt
       61 taagtccgat gttaaagact ggggctcaac cccagataca gcgttggata ctggcaggct
      121 tgacggatgg agaggcgagt ggaattacca gtgtagcggt ggaatgcgta gatactggta
      181 ggaacaccta ttgcgaaggc agctcgctgg acatatcgtg acgctgaggc gcgaaagtgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011001             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011001
VERSION     KHUT01011001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatca ctagggcgta aagggtgcgt aggtgggtta
       61 ataagtttcg tgtgaaatcc ctgggcttaa cctgggaact gcacgggaga ctgttagtct
      121 agagtacagg agaggtgagt ggaatttccg gtgtagcagt gaaatgcgta gagatcggaa
      181 ggaacaccgg tggcgaaggc gactcactgg cctgatactg acactcaagc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011002             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011002
VERSION     KHUT01011002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           82 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtccgca ggcggcgcgt
       61 taagtgaaat gcgaaatctt gtggctcaac catatagact gtgtttcata ctgacgtgct
      121 tgaggatggc agaggcaagt ggaatttctg gtgtagcagt gaaatgcgta gatatcagaa
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatatctg acgctcatgg acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011003             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011003
VERSION     KHUT01011003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           82 g           55 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttactg gtgggaaatc ggggcccaac cccgtggaag tcagtaatac tattaaacta
      121 gagggtcgga gaggttaacg gaatggttcg tgtaggggta aaatcctaaa atatgaattg
      181 gaacaccaaa cggcgaaggc agttaactat tcgattcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01011004             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011004
VERSION     KHUT01011004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          100 g           44 t
ORIGIN      
        1 gacggaggag gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcgggcaag
       61 caagtctggc gtgaaatctc caccgctcac cggggagggt gcgctggaaa ctacttgtct
      121 ggagttcggt agagggtcat ggaattccgc atgtagcggt ggaatgcgta gatatgcgga
      181 agaacacctg tggcgaaggc ggtggcctgg gccgaaactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011005             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011005
VERSION     KHUT01011005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           98 g           46 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggatcgg
       61 cgggtctggc gtgaaatctc caccacttaa tggggagagg ccgctggaaa ctaccgatct
      121 ggagtttggt agaggatcat ggaatttcag gtgtagcggt ggaatgcgta gatatctgaa
      181 agaacaccgg tggcgaaggc ggtgatctgg gccaaaactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011006             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011006
VERSION     KHUT01011006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcccat
       61 caagtcggat gtgaaagccc agggctcaac cttggaagtg catccgaaac tgatgagcta
      121 gagttcacgg agagggtggt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 ggaacaccgg tggcgaaggc gaccacctgg acggaaactg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011007             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011007
VERSION     KHUT01011007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           32 c           70 g           71 t
ORIGIN      
        1 tacgaagggg atgagcgttg ttcggaatga ttgggcgtaa agagttcgta ggctgtaatt
       61 gtagttacta gtaaaatcct aagtttatcg cttagaaatg cttgtaaaac tcttttactt
      121 gagtatgata gaggataaca gaatttcata cataggggta aaatccaata atttatgaag
      181 gaatgtcggt ggcgaaggcg attatctgga tcatttactg acgctgaggg acgaaagcgt
      241 agggagcaaa cagg
//
LOCUS       KHUT01011008             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011008
VERSION     KHUT01011008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           60 c           94 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggactgc
       61 cacgtcgggc gctgaaatcc cccggctcaa ccggggaatg ggcaccgata cgagcagtct
      121 tgaggggaat agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg gtttctactg acgctgagac gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011009             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011009
VERSION     KHUT01011009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           89 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggattta ctgggcgtaa agcgtgtgta ggcggcgcgt
       61 taagtgggac gcgaaatctt gtggctcaac catatagact gtgtcccata ctggcgtgct
      121 tgaggaaggc agaggcaagt ggaattaccg gtgtagcagt gatatgcgta gagatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccttatctg acgctgagac acgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011010             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011010
VERSION     KHUT01011010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           92 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttga
       61 taagttagct gtgaaagtcc tgggcttaac ctgggaaggt cagctaagac tgttgaactt
      121 agagtatagg agagggtagt ggaatttccg gtgtagcggt gaaatgcgta gagatcggaa
      181 ggaacaccag tggcgaaggc ggctacctgg cctaatactg acactgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011011             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011011
VERSION     KHUT01011011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c          100 g           39 t
ORIGIN      
        1 gacagagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggataca
       61 gcaagtccga agtgaaatct cgccgctcaa cggcgaggct gcttcggaga ctgcgtgtct
      121 ggggaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011012             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011012
VERSION     KHUT01011012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          106 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggtggcttgt
       61 taggttcgtc gttaaagccc ggggctcaac tccgggtggt cggcggagac cggcgggctt
      121 agaggccggt agagggatgc ggaattcccg gtgtagtggt gaaatgcgta gagatcggga
      181 ggaacaccag aggcgaaagc ggcatcctgg gccgagcctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011013             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011013
VERSION     KHUT01011013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c          102 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcggatcgg
       61 cacgtccgag gctgaaagcc cccggctcaa ccggggaagg ggcccggata cgaccggtct
      121 ggaggggggt agggggcgct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagcgcctgg gccccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011014             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011014
VERSION     KHUT01011014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c          101 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggccttt
       61 taagtctttc tgtgaaattc cggggctcaa ctccggggct gcaggggata ctgggaggct
      121 ggagacaagt agaggcagat ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccag aggcgaaggc ggtctgctgg gcttggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011015             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011015
VERSION     KHUT01011015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          104 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgggg
       61 taagcgtgcg gtgaaagctc ggggctcaac cccgagtcgg ccgtgcgaac tgctctcgct
      121 cgagcattgt agaggcagat ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggtctgctgg gcaatggctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011016             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011016
VERSION     KHUT01011016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c          101 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtccggt gctgaaagcc cccggctcaa ccggggaacg ggcacggata cgaccggcct
      121 ggaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg gcccttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011017             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011017
VERSION     KHUT01011017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           92 g           43 t
ORIGIN      
        1 tacagaggcc ccgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggacgtg
       61 taagtgcttt gtgaaagccc tgggctcaac ccaggaaccg cagggcatac tgcacgtctt
      121 agaggaagct agaggcgggt agaacgatcg gtggagcggt ggaatgcgta gagatcgatc
      181 ggaatgccaa gggtgaagac aacccgctgg ggctttcctg acgctgaggc acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01011018             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011018
VERSION     KHUT01011018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           90 g           52 t
ORIGIN      
        1 gacgaaccaa gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctct
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaattg ccttggatac tgtgaggctc
      121 gagtgaggta ggggtaacgg gaacatccgg tggagcggtg aaatgcgttg atatcggatg
      181 gaacgccggt ggcgaaagcg cgttactgga ccttttctga cgctgagtgc gcgaaagcca
      241 gggtagcgaa cggg
//
LOCUS       KHUT01011019             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011019
VERSION     KHUT01011019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           88 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggcttgg
       61 caagttcgac gtgaaatctc cgggcttaac ccggaaaatg cgttgaatac tgtttgagct
      121 cgaggtttgg aggggggact ggaattctcg gtgtagcagt gaaatgcgta gatatcgaga
      181 ggaacaccag tggcgaaggc gagtccctgg acaacacctg acactgaggc acgaaagccg
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011020             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011020
VERSION     KHUT01011020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggtttgg
       61 taagtttctt gtgaaatctg agggcttagc cctcaaagtg caagaaaaac tgccagactt
      121 tgagtgccag agaggatagt ggaattcccg gtgtagaggt gaaattcgta gagatcggga
      181 ggaacaccag tggcgaaggc ggctatctgg ctggcaactg acgctgaaac tcgaaagcat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011021             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011021
VERSION     KHUT01011021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgtgta ggcggaggtt
       61 taagtgcaat gtgaaatcgt gtggctcaac catatcgacg gtattgcata ctggatctct
      121 agaagacggc agaggcaagt ggaattagta gtgtagcggt aaaatgcgta gatattacta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccgtccttg acgctgagac acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011022             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011022
VERSION     KHUT01011022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           96 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcctt
       61 taagtctggt gtgaaaggtt cacggctcaa ccggaacagt gcattggata ctgagggact
      121 tgagttaggg agagggaagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agcttcctgg cctataactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011023             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011023
VERSION     KHUT01011023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           49 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggattta ttgggcgtaa agggctagta ggcggcttag
       61 caagtcgggt gtgaaaactc caggctcaac ctggagatgc catccgatac tgctatggct
      121 tgagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggtctgg gccggcactg acgctgaata gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01011024             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011024
VERSION     KHUT01011024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           53 t
ORIGIN      
        1 tacgtaggac ccaagcgtta tccggaatca ctgggcgtaa agcgtacgta ggcggaaatt
       61 taagtgatgt gcgaaatcct ggggctcaac cctatggact gtgcatcata ctgggtttct
      121 agaggatggc agaggcaagt ggaattggta gtgtagcagt gaaatgcgta gatattacca
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatatctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011025             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011025
VERSION     KHUT01011025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c          101 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtgat
       61 cgcgtcggtc gctgaaagcc cccggctcaa ccggggaagc ggcgccgaaa cgggtcgcct
      121 ggaggggagt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg gctctcactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011026             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011026
VERSION     KHUT01011026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           85 g           47 t
ORIGIN      
        1 tacggagaac tcaagcgtta cccggaatca ctgggcgtaa agcgttgcgt aggcggtttt
       61 gcaagtcgga tcttaaaggc caaggctcaa cctgggaact gggtacgata ctacaaaact
      121 ggaggatggt agaggtgagt ggaattgctg gtgtaggagt gaaatccgta gatatcagca
      181 ggaacaccaa tggcgaaggc agctcactgg gccattcctg acgctgaggc acgaaagcgt
      241 agggagcgaa cggg
//
LOCUS       KHUT01011027             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011027
VERSION     KHUT01011027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           81 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttattg gtcgatgcca aactcaattt gacggaagct ggtaatactg ttctactcga
      121 gggcaggaga ggttgatgga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaag aggcgaaggc agtcaactat tctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011028             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011028
VERSION     KHUT01011028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattca ctgggcgtaa agggcgtgta ggcggttccg
       61 taagtcagat gtgaaagctc ggggctcaac cccgacagtg cacctgatac tgcggtgact
      121 cgagggcggt agaggcagat ggaactcgcg gtgtagcggt ggaatgcgca gatatcgcga
      181 ggaacgccgg tggcgaaggc gatctgctgg gccgtccctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011029             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011029
VERSION     KHUT01011029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           49 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggtccgt
       61 taagtgcaca agtgaaagct cctggctcaa ccaggaaact gctttgcata ctggcgggct
      121 ggagggacgc agaggtctgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcagactgg gcgtttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011030             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011030
VERSION     KHUT01011030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggctttt
       61 taagtccggg gtgaaaggcg gtggctcaac catcagcagt gccctggata ctgggaagct
      121 cgagtaagtt tgaggccggt ggaacgggtg gtgtagcggt gaaatgcata gataccatcc
      181 agaacaccga ttgcggaggc agccggctaa gacttcactg acactgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011031             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011031
VERSION     KHUT01011031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta gacggctctt
       61 taagtcaggt gtgaaatccc tgggctcaac ctgggaatgg catctgatac tggagagctt
      121 ggattctgga agaggatggt ggaattcctg gtgtagcggt gaaatgcgta gagatcagga
      181 ggaacactag tggcgaaggc ggccatctgg tctaagaatg acgttgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011032             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011032
VERSION     KHUT01011032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           93 g           50 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 ttagtcaggt gtgaaatctc cacgctcaac gtggaggggc cacctgatac tgccatggct
      121 tgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccca tagcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011033             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011033
VERSION     KHUT01011033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           84 g           58 t
ORIGIN      
        1 gacataggtg gcgaatgtta tccggattta ttgggcgtaa aggatgcgta gatggtggaa
       61 taagttattg gtcgaggtca agctcaactt ggcggaagct gataatactg ttctacttga
      121 ggacaggaga ggttggcgga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaaa gggcgaaggc agccaactat tctgatcctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011034             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011034
VERSION     KHUT01011034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          109 g           36 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ctgggcgtaa aggacgcgca ggcggcccga
       61 ggggtctgtc gttaaagccc ggggctcaac cccgggtggt cggcggaaac cggcgggctt
      121 ggaggccggt agagggatgc ggaattcccg gtgtagtggt gaaatgcgta gagatcggga
      181 ggaacaccag aggcgaaagc ggcatcctgg gccgggcctg acgctgaggc gtgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01011035             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011035
VERSION     KHUT01011035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           99 g           43 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 taagtcgggt gtgaaacccc cgggctcaac ccggggacgc cacctgatac tgctgtgact
      121 ggagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg accggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011036             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011036
VERSION     KHUT01011036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           93 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtctat
       61 taagtctgag gtgaaaggtt caacgctcaa cgtgaacagt gccttggata ctggtagact
      121 tgagtgtggc agaggagtat ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaataccga tggcgaaggc agtactctgg gctaacactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011037             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011037
VERSION     KHUT01011037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           50 t
ORIGIN      
        1 gacgtagggg gcaagcgtta tccggattta ctgggcgtaa agcgcgcgta ggcggcttgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gcttcgcata ctggcaagct
      121 tgaggaaggc agaggttagt agaattcctg gtgtagcggt gaaatgcata gatatcagga
      181 ggaataccga cggcgaaggc agctaactgg gccttatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011038             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011038
VERSION     KHUT01011038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           82 g           60 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttactg gttgatgtca aactcaattt ggtggaagct ggtaatactg ttctgctaga
      121 gggcaggaga ggttgatgga attctgtgtg gagcagtgaa atgcgttgat ctacagagga
      181 acaccaagaa aggcgaaggc agtcaactat cctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011039             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011039
VERSION     KHUT01011039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           65 c           99 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgg
       61 cacgtcattg gctgaaatcc cccggctcaa ccggggaagt ggccgtgata cgaccgggct
      121 ggaggggggt aggggggacg ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gcgtccctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011040             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011040
VERSION     KHUT01011040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           92 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatcgt
       61 caagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tggcgatctt
      121 ggagtccggg agaggtgagc ggaactgcga gtgtagaggt gaaattcgta gatattcgca
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011041
VERSION     KHUT01011041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           95 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcgggg gtgaaatctt tcggcttaac cggaaaagtg ctttcgatac tggcgggctt
      121 gagcgaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011042             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011042
VERSION     KHUT01011042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           76 g           62 t
ORIGIN      
        1 tcccagcggt ccaagtcgta accacgttta ttgggtctaa aacatccgta gcttgtcttg
       61 taagtctctt gtgaaatcgg gcatcttaag tgtccggcga gcaagagata ctgcgaggct
      121 tgggactggg agacgtaaga agtacagttt gggtagcggt taaatgcgtt gatccttact
      181 ggactaacaa tagcgaaggc atcttacgag aacagatctg acagtgaggg atgaaggcta
      241 ggggcgcaaa ttgg
//
LOCUS       KHUT01011043             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011043
VERSION     KHUT01011043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           37 c           84 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttattg gtggatacca aactcaattt ggtgggagct agtaatactg ttcggctaga
      121 gggcaggaga ggttgatgga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaag aggcgaaggc agtcaactat tctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01011044             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011044
VERSION     KHUT01011044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          101 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggtcatt
       61 taagtccggg gtgaaaggtt cagcgctcaa cgcgaacagt gccctggata ctgggtgact
      121 tgagtctggc agggggtggc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggccacctgg gctaagactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011045             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011045
VERSION     KHUT01011045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 ttagtcgggt gtgaaacctc caggctcaac ctggagccgc catccgatac tgccatggct
      121 agagtccggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011046             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011046
VERSION     KHUT01011046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           98 g           45 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaatctc cgggctcaac tcggaggggc cactcgatac tgctatgact
      121 agagtccggt aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcgctctgg gccggaactg acgctaaaga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011047             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011047
VERSION     KHUT01011047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           95 g           51 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggctgtg
       61 caagtctgat gtgaaatctt caccgcttaa cgggaagagg tcattggaaa ctgcgcagct
      121 cgagtatggt agaggcgagt ggaattccag gtgtagcggt ggaatgtgga gatatctgga
      181 agaacaccag tggcgaaggc gactcgctgg gccattactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011048             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011048
VERSION     KHUT01011048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           87 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtggcg
       61 taagtcagtt gtgaaattcc ccggcttaac cggcgggact gcgattgata ctgcgttact
      121 tgaatcaggt tgaggtaggc ggaatgtgac atgtagcggt gaaatgctta gatatgtcat
      181 agaacaccga ttgcgaaggc agcttgctgg acctgtattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011049             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011049
VERSION     KHUT01011049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          102 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggc
       61 cgcgtcggtt gctgaaagtc cccggctcaa ccgggggatg ggcgccgata cgggccgtct
      121 ggaggggcgt agggggagct ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gagctcctgg acgccttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011050             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011050
VERSION     KHUT01011050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           97 g           40 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgg
       61 taagtcgggt gtgaaaactc tgggctcaac ccggagacgc cacccgatac tgccatggct
      121 agagtccgac aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcgctctgg gtcggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011051             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011051
VERSION     KHUT01011051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggccccg
       61 taagtcagtt gtgaaatccc ccggctcaac cggtgggact gcgattgata ctgtggggct
      121 tgaatcaggt tgaggcaggc ggaatgtggc atgtagcggt gaaatgctta gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg gcctgtattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011052             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011052
VERSION     KHUT01011052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           90 g           49 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggattca ttgggcgtaa agggctagta ggcggcttag
       61 caagtcgggt gtgaaatttc caggcttaac ctggaaccgc catccgatac tgctatggct
      121 agagtccggt aggggaccac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccga tggcgaaggc agtggtctgg gccggcactg acgctgaata gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01011053             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011053
VERSION     KHUT01011053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgtcatg
       61 caagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgaaac tgcaagactg
      121 gagtctcgga gaggaaagcg gaattctggg tgtagaggtg aaattcgtag atatccagag
      181 gaacaccggt ggcgaaggcg gctttctgga cgagttactg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011054             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011054
VERSION     KHUT01011054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttag
       61 caagtcgggt gtgaaaactc caggctcaac ctggagacgc cactcgatac tgttatggct
      121 tgagttcggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggtctgg gccgaaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01011055             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011055
VERSION     KHUT01011055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           98 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 ttagtcgggt gtgaaatctc caggctcaac ctggaggggt cattcgatac tgccatgact
      121 agagttcggt aggggtgtgt ggaattcctg gtggagcggt gaaatgcgca gagatcagga
      181 ggaacacccg tagcgaaggc ggcacactgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011056             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011056
VERSION     KHUT01011056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           44 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggcttgg
       61 caagtcgatt gtgaaacctc caggctcaac ctggagacgc cagtcgagac tgctatggct
      121 agagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011057             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011057
VERSION     KHUT01011057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           81 g           58 t
ORIGIN      
        1 tacagagggt gcaagcattg tccggattta ttgggcgtaa agcgtttcgt aggcggattt
       61 gcaagttatt tttcaaagac cagggctcaa ccctggtaag ggggataata ctacaagtct
      121 tgaaagttat cggggttttc ggaacttgcg gtgtaggggt gaaatccgtt gatatcgcaa
      181 ggaacaccaa gggcgaaggc agaaaactag gtaacatttg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01011058             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011058
VERSION     KHUT01011058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcgggcccg
       61 aacgtccgat gctgaaagcc cccggctcaa ccggggaagt ggcacggata cggcgggcct
      121 ggaggggggt agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagctcctgg acccttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011059             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011059
VERSION     KHUT01011059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           45 t
ORIGIN      
        1 tacggggggg ggcaagcgtt gttcggaatt actgggcgta aagggcgtgt aggcggccaa
       61 ctaagtccga cgtgaaatcc ccaggcttaa cctgggaact gcgtcggata ctggatggct
      121 tgaatccggg agagggatgc agaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaataccgg tggcgaaggc ggcatcctgg accggcattg acgctgaagc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011060             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011060
VERSION     KHUT01011060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           95 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcgggaaga
       61 tatgtcggga gtgaaatcgt acggcttaac cgtagcaatt gcttccgaaa ctgtttttct
      121 tgaatgcggg agaggcggat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccag tggcgaaggc ggtccgctgg cccgtgattg acgctgaggc gcgaaagtgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011061             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011061
VERSION     KHUT01011061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           48 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcagct gttaaagctc caggctcaac ctggagaggc cagttgatac tgctgtggct
      121 agagtccggt aggggagtgc ggaattcctg gtgtagcggt ggaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011062
VERSION     KHUT01011062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           96 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgtg
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gacataactg
      121 gagtaggtga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc accatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011063             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011063
VERSION     KHUT01011063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c           99 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtcgg
       61 tacgtcggtc gctgaaatcc cccggctcaa ccggggaacg ggcgccgaaa cggctggcct
      121 ggaggggggt aggggaatcg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gcgattctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011064             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011064
VERSION     KHUT01011064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           83 g           49 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtttttctt
       61 caggtctggt gtaaaagcac aggagctcaa ctccgtgtac gtgccggaaa ccaaagaaat
      121 agagtcattc agaggcatct ggaatgtcgt gtgtaggggt aaaatccgta gatccacgat
      181 ggaacgccaa aagcgaaggc aggatgctgg ggatgtactg acactcagag acgaaagcgt
      241 ggggagcaaa gggg
//
LOCUS       KHUT01011065             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011065
VERSION     KHUT01011065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           84 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtatgta ggtgggtgtt
       61 taagtgagat gcgaaatctg gcggctcaac cgtctagact gtatctcata ctgggcacct
      121 cgaggatggc agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gatatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccaaccctg acactgagat acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011066             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011066
VERSION     KHUT01011066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           39 c           79 g           57 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agagtgcgta ggtggttgaa
       61 taagttactg gtgggaaatc ggagctcaac tccgtggaag tcagtaatac tattgaacta
      121 gaggatcgga ggggttaacg gaattctatg tgtagcggta aaatgctcaa atatatagag
      181 gaacaccaaa tggcgaaggc agttaactat tcgactcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01011067             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011067
VERSION     KHUT01011067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           84 g           53 t
ORIGIN      
        1 tacgaaggcc ccaagcgtta tccggattta ttgggcgtaa agagcgcgta ggtggtccga
       61 tgcgtctcat cttaaatctc cgagctcaac ttggagttcg ggtgggatac ggtcggacta
      121 gagagtgtga gaggcaaacg gaacgcacgg tgtaggggtg aaatccgttg atatcgtgcg
      181 gaacaccaaa ggcgaaggca gtttgctggc acatttctga cactcagtgc gcgaaagcgt
      241 ggggatcaaa aagg
//
LOCUS       KHUT01011068             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011068
VERSION     KHUT01011068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           99 g           41 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggcccag
       61 taagtccagg ggtgaaatgc cggagctcaa ctccgtgccc gccccggata ctgttgggct
      121 agagacacgt agaggcaagc ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccgg aggcgaaggc ggcttgctgg gcgtgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011069             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011069
VERSION     KHUT01011069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           63 c           99 g           34 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccc
       61 taagcgccat cgtgaaatcc cgccgctcaa cggcggaacc gcgtggcgga ctgggggtct
      121 tgagggaccc aggggcgagc ggaacaggcg gtggagcggt gaaatgcgta gatatcgcct
      181 ggaacgccga cggtgaaaac agctcgctgg gggtcatctg acgctgagac acgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011070             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011070
VERSION     KHUT01011070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           99 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcggtctga
       61 gaagtctatg ggtgaaatgc cggggctcaa ctccgtgcat gcccaggata ctctcaggct
      121 agagacatgt agaggctggc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccag aggcgaaggc ggccagctgg gcatgatctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011071             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011071
VERSION     KHUT01011071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           52 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgaa gtgaaatctc cagagctcaa ctcggaaatt gcttcgcata ctggcgagct
      121 tgaggaatgc agaggttagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctaactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011072             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011072
VERSION     KHUT01011072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           97 g           43 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcaat
       61 caagtcaggc gtgaaaggcc tgggctcaac ccgggaactg cgcttgatac tggatgactc
      121 cgagtccggg agaggatggc ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggccatctgg accggcactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011073
VERSION     KHUT01011073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgca ggtggtttga
       61 taagatatct gtgaaatccc cgggctcaac ctgggaaggt cagataagac tgtcagactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011074
VERSION     KHUT01011074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcccag
       61 tcagtccgtt gtgaaagtca aaggctcaac ctttgaatgc cgatggatac tgctgggctt
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat agcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011075
VERSION     KHUT01011075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           92 g           51 t
ORIGIN      
        1 gacgaaccat gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtcgat
       61 caagtccgtg gtgaaatctt tcggcttaac cggaaaattg ccgtggatac tgattggctc
      121 gaagggggta ggggcagcgg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg cgctgctgga cctctcttga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011076
VERSION     KHUT01011076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           81 g           54 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ctgggcgtaa agggtgtgta ggcgacaaat
       61 caagttcgtt gttaaagatc tcggcttaac cgagaaagtg cgaagaatac tgatttgata
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atatcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011077             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011077
VERSION     KHUT01011077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           80 g           57 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttactg gtgggaaatc ggggctcaac ctcgtggaag tcagtaatac tgttgaacta
      121 gagggtcgga gaggttaacg gaatggctca tgtaggggta aaatcctcaa atatgagtta
      181 gaacaccaat cggcgaaagc agttaactat tcgactcctg acattgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01011078             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011078
VERSION     KHUT01011078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttacg
       61 gaagtcgggt gtgaaatatc ggggctcaac cccgggacgc cattcgatac tccgttgact
      121 cgagtccggt aggggaaggt ggaattcctg gtgtagcggt gaaatgcaca gatatcagga
      181 ggaacaccgg tggcgaaggc ggccttctgg gccggtactg acgctgagga gcgaaagcta
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011079
VERSION     KHUT01011079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttaa
       61 caagtcgatt gtgaaagccc ggggcttaac ctcggaagtg cagtcgaaac tgttagacta
      121 gagtgcagta gaggttggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gccaactggg ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011080
VERSION     KHUT01011080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           78 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtgtg
       61 aaagtcagtg gtgaaatctc agagcttaac tctgaaactg ccattgatac tttgcatctt
      121 gaattttctg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctgactacg gatgaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011081
VERSION     KHUT01011081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggtgatcctt
       61 caagtcttgt gtgaaatccc acggcttaac cgtggaagtg cacaagaaac tgtaggattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011082
VERSION     KHUT01011082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          100 g           44 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagtccggt gtgaaagccc ctggctcaac tggggaagtg cgccggatac tggcaggcta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgatgcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011083
VERSION     KHUT01011083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggtaaga
       61 tgtgtggaat gtgaaagccc cgggcttaac ctgggaagcg cataccaaac tatctaactg
      121 gagtaagtga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc acaatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011084
VERSION     KHUT01011084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 tgcgtcggtt gtgaaaccct ggggctcaac tccaggcctg caatcgatac gggcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgaaactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011085
VERSION     KHUT01011085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           86 g           51 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtccgg
       61 taagttaggt gtgaaatccc tcggctcaac cgaggaactg cgcttgatac tgccggactc
      121 gagggagata gaggaaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctttctggg tctcttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011086
VERSION     KHUT01011086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgca ggcggtttgg
       61 caagtgtcat gtgaaatccc cgggcttaac ctgggaactg catgacagac tgctggactg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011087
VERSION     KHUT01011087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           88 g           50 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggagtga ttgggcgtaa agcgcacgta ggtgggccag
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaacag catctgaaac tactagtctt
      121 gaatatctca gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcgga ggcgaaggcg gccaactggg agtatattga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011088
VERSION     KHUT01011088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           97 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcggag
       61 catgtcgggt gtgaaagccc tcggcttaac cgaggaagtg cgcccgaaac tgctttgctg
      121 gagtgccgga gagggtagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011089
VERSION     KHUT01011089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           50 c           73 g           54 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggtttag
       61 caagtcaggg gttaaaacct accgcttaac ggtaggatcg ctcttgatac tactagacta
      121 gagtacggaa gaggcaagcg gaattctcgg tgtagtcgta ataagcgttg atatcgagaa
      181 gaacaccaaa tgcgaaggca gcttgctagt acgtaactga cactcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011090
VERSION     KHUT01011090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c          101 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggcgcgc
       61 aacgtgggat gtgaaagcgg gcggctcaac cgcccgaggc catgccaaac gagcgtgctg
      121 gagccatgta gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggggaagcca gccacctggg catgggctga cactcaagtg cgaaagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01011091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011091
VERSION     KHUT01011091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           87 g           48 t
ORIGIN      
        1 cacgtaggag acgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtttgg
       61 aaagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaaaac taccagacta
      121 gagtacagca gaggggagtg gaattccgag tgtagcggta aaatgcatag atattcggaa
      181 gaacaccagt ggcgaaggcg gctccctggg ctgaaactga cgctcagacg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011092
VERSION     KHUT01011092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           38 c           77 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggaaag
       61 ttagtcagtg gtgaaaattc taggcttaac ttagaaattg ccattgatac tgcttttctt
      121 gagtccgatt gaagtggacg gaatatggca tgtagcggtg aaatgcttag agatgtcata
      181 gaacaccaat tgcgaaggca gttcactaag tcgtaactga cactgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011093
VERSION     KHUT01011093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           96 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgca ggcggtgcgt
       61 taagtcagat gtgaaaggct acagctcaac tgtagagttg catttgaaac tggcggactt
      121 gagtgcagga gaggtgagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actctctggc ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011094
VERSION     KHUT01011094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggcatgta ggcggtccgt
       61 taagtccagt gtgaaatctc ccggcttaac tgggagcggt cactggatac tggcagactt
      121 gaggggggta gaagaaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gccttctagg cctaccctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011095
VERSION     KHUT01011095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttgcg
       61 taagtgagat gtgaaatccc cgggcttaac ctgggacgtg catcttagac tgcgtagctg
      121 gagtgttgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaacactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011096
VERSION     KHUT01011096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           45 c           83 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agggcgcgta ggcggattgt
       61 taagtcagat gtgaaagccc ggagctcaac tccggaacag catttgaaac tagcaatcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctgatgcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01011097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011097
VERSION     KHUT01011097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           90 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 taagtctggt gtgaaatctc ggggcttaac ctcgaaatgg cattggatac tgcgttgctc
      121 gaggactgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011098
VERSION     KHUT01011098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgta ggcggctcac
       61 taagtcaaat gtgaaatccc tgggctcaac ctaggaagtg cattcgaaac tggtgagctt
      121 gaatcctgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg accacctgga cggagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011099
VERSION     KHUT01011099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           87 g           46 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtttcctgc
       61 cacgtctgga gtcaaatggc gatgcttaac atcgtcaacg ctccggaaac gagcgggata
      121 gagtcactca gaggtacctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgccgaa agcgaaggca gggtactggg ggtgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011100
VERSION     KHUT01011100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           40 c           87 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcatt
       61 taagtctgat ttgaaagcaa gcggcttaac cgtttgatgt ggttggaaac tgagtgactt
      121 gaatgggttg gcggtagccg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011101
VERSION     KHUT01011101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggtggattct
       61 taagtgggat gtgaaatccc ggggctcaac cccggaatgg cattccaaac tggggatcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011102
VERSION     KHUT01011102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           55 t
ORIGIN      
        1 tacagggggt gcaagcgtta ttcggaatta ttgggcgtaa agagcgcgta ggcggtctac
       61 taagtttggt gtgaaatccc tgggctcaat ccaggaagtg cattgaatac tggtagactt
      121 gagtattgga gaggtaagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaacagt ggcgaaggcg gcttactgga caattactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011103
VERSION     KHUT01011103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           84 g           52 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggaatta ctgggcgtaa agcgcatgta ggcggacatt
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaattg cattcgatac tgaatgtcta
      121 gagtatgata gaggagagtg gaacttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gctctctgga tcaatactga cgctaaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011104
VERSION     KHUT01011104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacgtagggt gctaacgttg ttcggaatca ctgggcataa agagcgcgta ggcggtctaa
       61 taagtccagc gtgaaagccc ccggcttaac cggggaattg cactggatac tgttggacta
      121 gagtacagga ggggagagcg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggc ggcgaaagcg gctctctggt ctgtcactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011105
VERSION     KHUT01011105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 cacgtaggag acgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcggg
       61 taagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaaaac tgccgggcta
      121 gagtgcaaca gaggggagtg gaattccgag tgtagcggta aaatgcatag atattcggaa
      181 gaacaccagt ggcgaaggcg gctctctggg ttgcaactga cgctcagacg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011106
VERSION     KHUT01011106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           47 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggagac tgctggtctt
      121 gagggaggta ggggcctgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcgggctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011107             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011107
VERSION     KHUT01011107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           89 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcctag
       61 taagtcagtt gtgaaattcc ccggcttaac cggtgggact gcgattgata ctgctgggct
      121 tgaatcaggt tgaggtaggc ggaatgtggc atgtagcggt gaaatgctta gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg gcttcgattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011108             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011108
VERSION     KHUT01011108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtgtg
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatacatctt
      121 gaatgcccgg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaca ggggttattg acgctgaggc acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01011109             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011109
VERSION     KHUT01011109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           49 t
ORIGIN      
        1 gacgtggggt gcaagcgttg tccggagtga ctgggcgtaa agggcgtgta ggcggttttg
       61 tgagtcttct ttgaaagctc tcggctcaac cgagcagccc ggaggagata ctataagact
      121 agacggcaag aggggcagac agaattcccg gtgaaggggt aaaatccgtt gatatcggga
      181 ggaataccag tggcgaaggc ggtctgctag cttgcccgtg acgctgaggc gcgacagtct
      241 gggtagcgaa aggg
//
LOCUS       KHUT01011110             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011110
VERSION     KHUT01011110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           64 c          101 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgg
       61 cacgtcgaat gctgaaagcc cccggctcaa ccggggaaga ggcgtcgata cggccgggct
      121 ggaggggggt aggggaaccg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg cggcgaaagc gcggttctgg accccgcctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011111             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011111
VERSION     KHUT01011111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           52 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggcgcgt
       61 taagtgcaac gcgaaatccg gtggctcaac catctggact gtgttgcata ctggcgtgct
      121 tgaggatacg agaggtacat ggaattagcg gtgtagcagt gaaatgcgta gatatcgcta
      181 ggaacaccaa tggcgaaggc agtgtactgg cgtattcctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011112             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011112
VERSION     KHUT01011112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           97 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcgcgta ggcggattgc
       61 tacgtcggtt gctgaaatcc cccggctcaa ccggggaagt ggcagcgata cgggcagtct
      121 cgagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtcttctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011113             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011113
VERSION     KHUT01011113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           95 g           48 t
ORIGIN      
        1 cacgtaggca ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaaacct tgggctcaac ccaaggactg catccgatac tgctgtgact
      121 tgagtccggt aggggagtgg ggaattcctg gtgtagcggt ggaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011114
VERSION     KHUT01011114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           94 g           48 t
ORIGIN      
        1 gacgtagggg gctagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggtccgt
       61 taagtgtaga gtgaaagctc cgggctcaac ccggaaaccg ctctgcatac tggcgggctg
      121 gagggacgca gaggtctgtg gaattcctgg tgtaacggtg aaatgtgttg atatcaggag
      181 gaacacccat ggcgaaggca gcagactggg cgtttcctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011115             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011115
VERSION     KHUT01011115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           67 c          101 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggacgg
       61 cgcgtcggcc gctgaaatcc cccggctcaa ccggggaagt ggcgccgata cggccgccct
      121 ggagcgacgt aggggggacc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggtccctgg acgtcggctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011116             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011116
VERSION     KHUT01011116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c          100 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgc
       61 cacgtcgggc gctgaaatcc cccggctcaa ccggggaagg ggcaccgata cgagcggcct
      121 ggaggggagt aggggggcct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg actctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011117             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011117
VERSION     KHUT01011117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           67 c           99 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgc
       61 cgcgtcgaac gctgaaatcc cccggctcaa ccggggaacc ggcgtcgata cgggcggcct
      121 cgaggggggt aggggggcct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011118             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011118
VERSION     KHUT01011118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           97 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcggttttc
       61 taagtttggt gtgaaaggtt cagggctcaa cccgaacagt gcactgaata ctgggaaact
      121 tgagtggggg agaggaagat ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccgg tggcgaaggc ggtcttctgg cctcttactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011119             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011119
VERSION     KHUT01011119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           99 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggcttgg
       61 caagtcgggt gtgaaatctc tgggcttaac tcagagaggc cactcgatac tgctatggct
      121 agagtccggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011120             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011120
VERSION     KHUT01011120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           60 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta gacggatgac
       61 cgcgtcgagt gctgaaaccc ctcggctcaa ccgaggaagg ggcctcgaaa cgggtcgtct
      121 ggagtggagg agggggaccc ggaacggccg gtggagcggt gaaatgcgtt gagatcggcc
      181 ggaacgccaa tggcgaaagc agggtcctgg actctcactg acgttgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011121
VERSION     KHUT01011121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctccg
       61 atagtcgggg gtgaaatccc acagcttaac tgtggaagtg ccttcgatac tgcggggctc
      121 gagcgaggta ggggtcgagg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011122
VERSION     KHUT01011122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           85 g           49 t
ORIGIN      
        1 tacatagacc tcgagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtcctgc
       61 taagtctccg gtgaaagccc ccggcccaac tggggaactg tcggagaaac tagcaggatc
      121 gagtgtgaca gaggcagacg gaattcttgg tgtagcggta aaatgcgttg atatcaagag
      181 gaacaccgat ggcgaaggca gtctgctggg tcatcactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011123
VERSION     KHUT01011123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaataa ctgggcttaa agggagcgta ggcgggctaa
       61 caagtctggg gtgaaatccc gcggctcaac cgtggaactg ccttagatac tgtcggcctc
      121 gagggagata ggggcatgcg gaactaacgg tggagcggtg aaatgcgttg atatcgttag
      181 gaactccggt ggcgaaagcg gcgtgctgga tctcttctga cgctgaggct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011124
VERSION     KHUT01011124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          102 g           37 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggcgcgc
       61 acagtcgggc gtgaaatccc cgggctcaac ccgggggctg cgcccgagac gtgcgggcta
      121 gaggatggaa gagggccgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcggcctggt ccatttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011125
VERSION     KHUT01011125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          107 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatca ctgggtgtaa agcgcgtgta ggcggcctgc
       61 caagtctgct gtgaaagcgc ctggcttaac cgggcgaggt cggtggaaac tggcgggctg
      121 gaggtcggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccccctggg ccgtacctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011126
VERSION     KHUT01011126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           81 g           57 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggtggttcgc
       61 taagttagat gtgaaagccc agggcttaac cctggaattg catttaaaac tggcgaactt
      121 gaagatcaca gaggtaagca gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaataccaac ggcgtaggca gcttactggg tgatacttga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011127
VERSION     KHUT01011127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 gacgaaccga gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtctgtg gtgaaatcct ccagctcaac tggagaacgg ccgcggatac tggtggtctc
      121 gaggaaggta ggggcatctg gaactggtgg tggagcggtg aaatgcgttg atatcatcag
      181 gaactccggt ggcgaaggcg agatgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011128
VERSION     KHUT01011128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          102 g           40 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggtccgc
       61 taagttcggt gtgaaagccc ccggctcaac tggggaaggg cgccggatac tggcggacta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011129
VERSION     KHUT01011129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttggg
       61 caagtcggat gtgaaagccc agggctcaac cctggaagtg cattcgatac tgctcggcta
      121 gagtcccgga gagggtggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacacctgt ggcgaaggcg gccacctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011130
VERSION     KHUT01011130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          106 g           37 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgtcta ggcggcccgt
       61 taagtccgct gtgaaaggcc ggggctcaac cccggaaggt cagcggagac tggggggctc
      121 gagtgccgga gagggatgcg gaatacctgg tgtagcggtg gaatgcgtag agatcaggtg
      181 gaacaccgat ggcgaaggca gcatcctggc cggagactga cgctgaagcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011131
VERSION     KHUT01011131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcgact gtgaaaaccc aaggctcaac cttgggcttg cagtcgatac gggcagactt
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccataactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011132
VERSION     KHUT01011132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           52 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcccgc
       61 taagtcaggg gtgaaagacg gtggctcaac catcgcagtg cccttgatac tggcgggctt
      121 gaattgacta gaggcaggcg gaatgtggca agtagcggtg aaatgcatag atatgccaca
      181 gaacaccgat tgcgaaggca gcttgctatg gtcatattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01011133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011133
VERSION     KHUT01011133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggatggt
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tggccgtctt
      121 gagtgccgga gaggaccagg gaacttcacg tgtagcggtg aaatgcgttg agatgtgaag
      181 gaacaccaat ggcgaaagca ctggtctgga cggagactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01011134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011134
VERSION     KHUT01011134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           90 g           53 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtctgta ggtggttgtt
       61 caagtctgct gttaaagcgt ctggcttaac cagataaagg cggtggaaac tgaacgacta
      121 gagtgcggta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctgt ggcgaaagcg ctctactgga ccgcgactga cactgatgga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01011135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011135
VERSION     KHUT01011135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 gacgagctct ccaaacgtta ttcggtatca ctgggcttaa agagttcgta ggcggcattg
       61 caggtgaggt gtgaaagccc tcggcttaac cgaggaattg cgcttcaaac cgcaaagcta
      121 gagggagaca ggggtgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011136
VERSION     KHUT01011136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           82 g           53 t
ORIGIN      
        1 tacgagtggt gcaagcatta ttcggaatca ttgggcgtaa agggtgcgta ggtggcaaac
       61 taagtctact gttaaatgtc tcggcctaac cgagaacctg cagtggatac tggtatgcta
      121 gaggatggga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaagca gcttcttggc ctgatcctga cactgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011137
VERSION     KHUT01011137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           81 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggatatt
       61 taagtcatat gtgaaagccc cgggctcaac ctgggaattg catctgaaac tggatatcta
      121 gagtatggta gaggatagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg actatctggg ccaatactga cgctgaggta cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011138
VERSION     KHUT01011138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggctgcgagg
       61 taagtctggt gtgaaatccc ggggctcaac cccggaactg cactggatac tgctttgcta
      121 gagcactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagttgctga cgctgaggca cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011139
VERSION     KHUT01011139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           85 g           51 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggttgtg
       61 taagtcgggt gtgaaattcc ggagcctaac tccggaactg cattcgaaac tacacagctc
      121 gaggtctgga gaggtgactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaagcg agtcactgga cagaacctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011140
VERSION     KHUT01011140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           92 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ttgggcgtaa agcgcgtgta ggcggttcac
       61 caagtccgct ctgaaagccc agggctcaac cctgggaggc gggtggatac tggtggacta
      121 gagtccggaa gaggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac agcgaaggca gatctctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011141
VERSION     KHUT01011141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           93 g           51 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggctctt
       61 taagtcagac gtgaaaggcc tgggcttaac ctaggaactg cgtttgatac tggagggcta
      121 gaagacggga gagggaagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccgga ggcgaaggcg gcttcctgga ccgatcttga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011142
VERSION     KHUT01011142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           91 g           47 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgat
       61 caagtcggtg gtgaaatcct gcagctcaac tgcagaacag cctccgatac tggtcgtctc
      121 gaggaaggta ggggtgtgtg gaacagttgg tggagcggtg aaatgcgttg atatcaactg
      181 gaactccggt ggcgaaggcg acacactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011143
VERSION     KHUT01011143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           92 g           54 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatta ctgggtgtaa agggagcgta ggcgggatcg
       61 caagtcagat gtgaaataca tgggcttaac ccatgggctg catttgaaac tgcggttctt
      121 gagtgaagta gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttactggg cttttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011144
VERSION     KHUT01011144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggcctgt
       61 taagtctgaa gtgaaaatcc agggctcaac cctggaactg cttcggatac tggtgggctt
      121 gagtgctcta gagggtagtg gaattccggg tgtagcagtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg actgcctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011145
VERSION     KHUT01011145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          101 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcga
       61 caagtcaacg gtgaaagctc ggggctcaac cctgagtcgg ccgtcgatac tgttgggctt
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011146
VERSION     KHUT01011146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           82 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggagttc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tggatctctt
      121 gagctatcta ggggtcactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggtgactgga gatatgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011147
VERSION     KHUT01011147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg ccctcgatac tggaagtctt
      121 gagtatggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011148
VERSION     KHUT01011148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           99 g           36 t
ORIGIN      
        1 gacagaggag gcaagcgttg tccggattaa ctgggcgtaa agcgtccgca ggtggcacgg
       61 ctagtggggt gtgaaaacgc gcggcttaac tgcgcgaggc catcccaaac tgccgcgctg
      121 gagccgggca gagggtgacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaagaccaag ggggaagcca gtcacctggg cccgtgctga cactcatgga cgaccgcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011149
VERSION     KHUT01011149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccggg
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctttgatac tgctcggctc
      121 gagggaggta ggggcggacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gtccgctagg cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011150
VERSION     KHUT01011150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           98 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgcat
       61 ttagtcaggc gtgaaattcc tgggcttaac ctggggactg cgcttgatac gggtgtgctt
      121 gaggacggaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccgtatctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011151
VERSION     KHUT01011151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           93 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggtcctt
       61 caagtcagat gtgaaagccc cgggctcaac ctgggaacag catttgagac tggaggactt
      121 gaggacggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccaat ggcgaaggca gctctctgga ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011152
VERSION     KHUT01011152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccgtt
       61 caagtcgggg gtgaaatccc acggcccaac cgtggaaggg cctccgatac tgaagggctc
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acctgctgga cctctgctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011153
VERSION     KHUT01011153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgg
       61 taagtcagat gtgaaagccc ggggctcaac cccggaagtg catttgaaac taccaggcta
      121 gagtgcagta gagaatggcg gaattcccag tggagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatttggg ctgtaactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011154
VERSION     KHUT01011154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           53 c          105 g           45 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggtggttgtc
       61 taagtccggt gtgaaatctc ccggctcacc tgggaggggg cgctggagac tgggcaactg
      121 gaggcaggca gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gccggctggg cctgtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011155
VERSION     KHUT01011155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggttgat
       61 caagtctgat gtgaaagccc ggggctcaac cccgggactg cattggaaac tgattagctg
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011156
VERSION     KHUT01011156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgccta ggcggtttga
       61 taagtccgct gtgaaagacc gaggctcaac ctcggggtgt cagcggagac tgttagactg
      121 gagtatcgga ggggagtgcg gaatacctgg tgtagcggtg gaatgcgtag atatcaggtg
      181 gaacaccgat ggcgaaggca gcactctggc cgaatactga cgctgaagcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011157
VERSION     KHUT01011157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c          101 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggtggttttc
       61 taagaccgat gtgaaatctc cgcgcttacc gtggagaggt cgttggtgac tgggagactt
      121 gagtttggca gaggtgagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcactggg ccatgactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011158
VERSION     KHUT01011158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          109 g           34 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 tgcgcgtgcc gtgaaagccc ggggctcaac cccgggtcgg cggtgcgaac ggccgtgctg
      121 gagcgtacga gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggc gtacggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011159
VERSION     KHUT01011159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c           99 g           37 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgtccgca ggcggctggg
       61 cacgtggggt gtgaaaccgc tcggcttaac cgagcgtcgc cacctcatac ggcccggctc
      121 gagcgcggga gaggtctgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggcgaaagca gcagactggc ccgtggctga cgctcaggga cgaaagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01011160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011160
VERSION     KHUT01011160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           91 g           53 t
ORIGIN      
        1 tacgggggat gcgagcgtta tccggattca ttgggtttaa agggtgcgta ggctgtgcgt
       61 caagtcgggg gtgaaattcc ggtgctcaac accggggctg cccttgatac tgtcgcgctg
      121 gagtgcggat gccgccggag gaatgagtgg tgtagcggtg aaatgcttag atatcactca
      181 gaacaccgat tgcgaaggca tctggcgaat ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatagaac agg
//
LOCUS       KHUT01011161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011161
VERSION     KHUT01011161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           98 g           44 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggcacta
       61 aaagtactca gtgaaatcca tctgctcaac gggtggggtg gtgagtagac tttagagcta
      121 gagagcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaagacccgt ggcgaaggcg gccccctggg ccgtatctga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011162
VERSION     KHUT01011162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggccttg
       61 taagtcagag gtgaaagctt cccgcttaac gggagaactg cctttgatac tgcaaagctt
      121 gaatcggcac gaggcaggcg gaatgtggca tgtagcggtg aaatgcttag atatgccata
      181 gaacaccaat tgcgaaggca gcctgccaga gccgtattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011163
VERSION     KHUT01011163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           97 g           40 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggaccgt
       61 taagtgtctt gtgaaatccc ccggctcaac cggggaaccg ctgggcatac tggcggtctt
      121 gggccaccta ggggtcatcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgac ggtgaaaaca gatgactggg ggtgtgccga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011164
VERSION     KHUT01011164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcctcg
       61 caagtctgat gtgaaatccc tcggctcaac cgaggacgtg catcggaaac tgcggggctt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011165
VERSION     KHUT01011165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           97 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggttcc
       61 tcagtccgga gtgaaagccc acggctcaac cgtggaagtg ctttggatac tgggaacctc
      121 gaggggggta ggggcgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cccccactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011166
VERSION     KHUT01011166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacgtaggac gcaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcgggaagg
       61 taagtcagtg gtgaaatctc acggctcaac cgtgaaactg ccgttgatac tgcttttctt
      121 gagtttggga gaggtcgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcgactggc ccataactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011167
VERSION     KHUT01011167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           89 g           49 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agcgcgcgta ggcgggcatc
       61 caagtcggtt gtgaaatccc ccagctcaac tggggaactg catccgatac tgtttgtctt
      121 gaggacggga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg actctctggc ccgtatctga cgctgaggcg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011168
VERSION     KHUT01011168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttga
       61 caagttgggt gtgaaatccc tcggcttaac cgaggaactg cattcaaaac tatcagactt
      121 gagtatcgga gggggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gccacctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011169
VERSION     KHUT01011169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgct
       61 taagtcagtt gtgaaatatc ccggctcaac cgggagggtg cgattgatac tgggcggctc
      121 gagtaacagc gaggttggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agcgaaggca gccaactagg ggttaactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01011170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011170
VERSION     KHUT01011170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           55 c          106 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggccgt
       61 taagtgggag gtgaaaggcc ggggctcaac cccggcagtg cctcccatac tggcggtctt
      121 gagggcggta gggagaagcg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg ccgttcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011171
VERSION     KHUT01011171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           99 g           35 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgcc
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaacgg ctgggcagac tgggcgtctt
      121 gaggggccca agggcggacg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggcgaaagca gtccgctggg ggccatctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011172
VERSION     KHUT01011172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggaccg
       61 taagtccgag aggaaatctc aaggctcaac tttggggctt tcttggatac tgcggttctt
      121 gaggatgaga gaggtaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcttactggc tcattcctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011173             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011173
VERSION     KHUT01011173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          102 g           45 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggaccgg
       61 taagtctggc gtgaaatctc catcgctcaa cgaggagagg tcgctggata ctgccggtct
      121 ggagtgcggc agaggaccat ggaattccag gtgtagcggt ggaatgcgta gatatctgga
      181 agaacaccgg tggcgaaggc ggtggtctgg gccgttactg acgctgagga gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011174
VERSION     KHUT01011174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggacttt
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctttgatac tgagggtctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011175
VERSION     KHUT01011175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           98 g           36 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggaccgt
       61 taagtgtccc gtgaaatccc ccggctcaac cggggaacgg cggggcatac tggcggcctt
      121 gagcaaccta ggggcgagcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccaac ggtgaagaca gctcgctggg ggtttgctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011176
VERSION     KHUT01011176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           99 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtctg
       61 caagtctgcg gtgaaagccc ccagcttaac tggggaagtg ccgtggatac tgcgggtctc
      121 gaggagggta ggggcatctg gaacattggg tggagcggtg aaatgcgttg atatccaatg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011177
VERSION     KHUT01011177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          104 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agggcgcgta ggcggggcgt
       61 caagtccgct gtgaaagccc ggagcttaac tccggtaggt cggtggagac tggcgctctt
      121 gaagaggcta gagggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacacccgt ggcgaaggcg gtctcctggg gccttcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011178
VERSION     KHUT01011178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgca ggctgttagt
       61 taagttgggt gtgaaatccc tgggctcaac cgaggaagtg catccaaaac taactaacta
      121 gagtatggca gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ccattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011179
VERSION     KHUT01011179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggttgttagg
       61 taagtcgatt gtgaaatccc tgggctcaac cgaggaagtg cagtcgaaac tacctagcta
      121 gaagacggca gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctgga ccgatcttga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011180
VERSION     KHUT01011180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgta ggcggctttg
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg catccgaaac tgcatggctt
      121 gagtcccgga gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg accacctgga cgaggactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011181
VERSION     KHUT01011181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcgggctcg
       61 tatgtcgatt gtgaaagccc tgggcttaac ccgggaatgg cagtcgaaac tgcgagtctt
      121 gaatgcttga gaggatggcg gaattcccgg tgtagaagtg aaattcgtag atatcgggaa
      181 gaacacctga ggcgaaggcg gctatctggc gatgcattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011182
VERSION     KHUT01011182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggatata
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gataaatctg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011183
VERSION     KHUT01011183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggattta ctgggtgtaa agggcgcgta ggcggggatg
       61 caagtcagat gtgaaatcta tgggcttaac ccataaactg catttgaaac tgtatctctt
      121 gagtgtcgga gaggtagacg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gtctactgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011184
VERSION     KHUT01011184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agggttcgta ggcgggagaa
       61 tatgtcggat gtgaaatcct caggctcaac ctgagacgtg catccgaaac tgtttttctt
      121 gaatgttgga gagggaagtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaagcg acttcctggc caaacattga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011185
VERSION     KHUT01011185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatta ttgggtgtaa agggcgcgta ggcggcgcga
       61 aaagtctgtt gtctaaactt ctggcctaac cgggagcatg cagtggatac tttcgtgctt
      121 gagtatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacgccaat ggcgaaggca gcttcttggt acattactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011186
VERSION     KHUT01011186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           93 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagttagat gtgaaatccc cgggctcaac tcgggtgttg catttaaaac tggtaggctt
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011187
VERSION     KHUT01011187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           87 g           52 t
ORIGIN      
        1 gacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggattgg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tgcttatctt
      121 gggccactta ggggttaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtaactggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011188
VERSION     KHUT01011188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           87 g           53 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agagaacgta ggtggtcctt
       61 taagttcgat cttaaagacc ggggctcaac cccgggaatg gattgaaaac tgggggacta
      121 gaggatgtta gaggacggtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg gccgtctggg acattcctga cactgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01011189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011189
VERSION     KHUT01011189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaaga
       61 taagtcagat gtgaaagccc aaggctcaac cttgggactg catttgaaac tatctttctt
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011190
VERSION     KHUT01011190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 cacgtaagtt gcgagcgttg ttcggaatta ttgggcgtaa agggcatgta ggcggtcgtg
       61 caagcttggt gtgaaatccc ggggctcaac tccgggactg cgttgagaac tgcacggcta
      121 gagtaactga ggggcagccg gaattccagg tgtaggggtg aaatctgtag atatctggaa
      181 gaacaccaat ggcgaaggca ggctgccagc agattactga cgctgaggtg cgaaggtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01011191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011191
VERSION     KHUT01011191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagcg cttctgatac tggcgggctg
      121 gagggaggta ggggcgactg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg agtcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011192
VERSION     KHUT01011192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctacg
       61 caagtcggat gtgaaagccc gcggctcaac cgcggaagcg cactcgatac tgcgcagcta
      121 gagtcctgga gaggaaggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011193
VERSION     KHUT01011193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          104 g           40 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcagtc
       61 taagtcctct gtgaaatcac ccggctcaac tgggtggctg cggaggagac tgggcagctt
      121 gaggggccta gaggccggtg gaactcccgg tgtagtggtg aaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg gccggctggg ggcgccctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011194
VERSION     KHUT01011194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           96 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggtcttt
       61 taagtcaggt gtgaaagccc ccggctcaac tggggagttg catctgatac tgggagacta
      121 gagtgcagga gaggaaagtg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgcaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011195
VERSION     KHUT01011195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           62 c          106 g           37 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agcgcacgta ggcggtcggt
       61 tgcgtcgctg ctgaaagcgc cgggcttaac ccggcgaggc gtggcgatac gagccgactc
      121 gaggcaggca gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgcctgt ggcgcaagcg gccacctggg cctgtcctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011196
VERSION     KHUT01011196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgtgta ggaggtcatg
       61 taagtcgggt gtgaaatccc atcgcttaac gatggaatgg cattcgatac tgcgtgacta
      121 gagggctaga ggggtaagcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccggt ggcgaaggcg gcttactaga tagctcctga ctctgaaaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011197
VERSION     KHUT01011197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           82 g           55 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggatgtt
       61 taagtgtgat gttaaatcgt gtggctcaac catatcgatg tgtcgcatac tgggcatcta
      121 gaggacagca gaggcaagtg gaactggtag tgtagcagtg aaatgcgtag atattaccaa
      181 gaacaccaat ggcgaaggca gcttgctggg ctactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011198
VERSION     KHUT01011198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           84 g           58 t
ORIGIN      
        1 tacgagaggt gcgagcgtta ttcggaataa ttgggtgtaa agggtgcgta gacggtgtgt
       61 taagtttatt gttaaatctt ccggctcaac cggaaatatg cagtgaatac tggcatgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011199
VERSION     KHUT01011199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c          102 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtgga
       61 taagtctgct gtgaaagccc ggggctcaac cccggagggt cggtggatac tatctgtctt
      121 gaaggtgcca gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctgga gcatacttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011200
VERSION     KHUT01011200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatgac
       61 caagtcaggg gtgaaatctt tcggcccaac cggaaaagtg cctctgatac tggtcgtctc
      121 gagggaggta ggggtcgagg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011201
VERSION     KHUT01011201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           97 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcatgg
       61 caagtcagga gtgaaagccc ggggctcaac cccggaatgt cttttgagac tgccaagcta
      121 gagtgcggaa gaggcaactg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011202
VERSION     KHUT01011202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           92 g           43 t
ORIGIN      
        1 gacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcacc cgtggaactg ctgggcatac tggcgatctt
      121 gggccactca ggggcagctg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgaa ggtgaaaaca ggctgctggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011203
VERSION     KHUT01011203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           93 g           40 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccgcg
       61 caggtcgggc gtgaaatccc gtggctcaac cacggaagtg cacccgaaac cgcgtagctg
      121 gagtctcgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg accttctgga cgatgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011204
VERSION     KHUT01011204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggttgag
       61 taagtcagtt gttaaagcgt ggagcttaac tccataaagg caattgaaac tactcggcta
      121 gagtgcgata ggggcaaggg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg ccttgctggg tctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01011205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011205
VERSION     KHUT01011205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           97 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgtacgca ggcggtcgcg
       61 ggcgtcggtg ctgaaagtcc accgcttaac ggtgggatgc gtgccgatac gccgcaactc
      121 gagccatgta gaggtccgtg gaattgccgg tgtagtggtg atatgcgtag atatcggcag
      181 gaacacccaa ggggaagcca acggactggg catgggctga cgctcaggta cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01011206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011206
VERSION     KHUT01011206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           89 g           44 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaacgg cttggcatac tgggagtctt
      121 gagccatcta ggggcgactg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaaaaca ggtcgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011207
VERSION     KHUT01011207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           97 g           40 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggagtta ctgggcgtaa agcgcccgca ggcggtcctg
       61 tgcgtggtgg gtgacagtcc tcggcttaac tgaggaagcg cttgccaaac ggcaggactt
      121 gaggaccaga gagggagacg gaactccggg tggagcggtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgtaggca gtctcctggc tggtatctga cgctcagggg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011208
VERSION     KHUT01011208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggtggtttgg
       61 taagtctttt gtgaaagctc ctggctcaac tgggagaggt caaaggaaac taccaggcta
      121 gagtttggta ggggagactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctat ggcgaaagca ggtctctggg ccacaactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011209
VERSION     KHUT01011209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           99 g           44 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggtggtggtt
       61 caagtggtgt atgaaatcgc tcggcttaac cgggcggggt tatgccagac tgagccactg
      121 gaggacggga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccagc ggcgaaggcg gcgccctggc ccgtacctga cactcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011210
VERSION     KHUT01011210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 aaagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac ttctgagctt
      121 gagtatcgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cgataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011211
VERSION     KHUT01011211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           42 t
ORIGIN      
        1 gacgaaccgc ccgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgag
       61 caagtcaggg gtgaaagccc ccagcttaac tggggaagtg cctctgatac tgcttgtctt
      121 gagggagata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011212
VERSION     KHUT01011212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           96 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgac
       61 caagtcgggg gtgaaatact tcagcttaac tggagaattg ccttcgatac tggttgtctt
      121 gagtggggta ggggcggacg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gtccgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011213
VERSION     KHUT01011213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           51 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 tatgtcttct gtgaaatctc caggctcaac ttggagtctg caggggaaac tgccgtgctt
      121 gagtatcgga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga cgataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011214
VERSION     KHUT01011214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           75 g           65 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggctgat
       61 taagtcagtg gtgaaagttt gtcgctcaac gataaaattg ccattgaaac tggttagctt
      121 gagtatgttt gaggtaggcg gaatgcgtag tgtagcggtg aaatgcatag atattacgca
      181 gaactccaat tgcgaaggca gcttactaaa ccataactga cactgaagca cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01011215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011215
VERSION     KHUT01011215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           40 c           72 g           59 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggaatta ttgggcgtaa agcgtctgca ggtggatata
       61 aaagtctttt gttaaatctt caggcttaac ctgaaatctg caaaagaaac tatgtatctt
      121 gagtatggta gaggtaaagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaacaccaac agcgaaggca ctttactggg ccagtactga cactcagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011216
VERSION     KHUT01011216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggcagcg
       61 caagtcgggc gtgaaatccc acggctcaac cgtggaagtg cactcgaaac tgcgcagcta
      121 gagtcctgga gaggaaggtg gaatgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011217
VERSION     KHUT01011217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           76 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcagag gtgaaaactt gcagctcaac tgtaagcttg cctttgatac tgatgggctt
      121 gaataaacta gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactatg gtttaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011218
VERSION     KHUT01011218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           89 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggctttg
       61 taagtctgat gtgaaagccc agggctcaac cctggaagtg cattggatac tgcgaagcta
      121 gagtattgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg caataactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011219
VERSION     KHUT01011219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           39 c           82 g           53 t
ORIGIN      
        1 tacatagggg gcaagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggttagg
       61 aaagtcagat gtgaaaaccc tgagctcaac ttagggaatg catttgaaac ttcttgacta
      121 gagtatggga gaggtaagtg gaatttctag tgtagcggta aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ccaaaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011220
VERSION     KHUT01011220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           76 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggatctt
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaatag ccattgaaac tgaagatctt
      121 gagtacagtt gaagtagata gaatgtatcg tgtagcggtg aaatgcttag atatgataca
      181 gaataccgat agcgaaggca atttgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011221
VERSION     KHUT01011221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c          107 g           34 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca gacggcacgg
       61 tatgtgcccg gtgacatcct acggcttaac cgtgggcggc ccgggtagac agccgtgctt
      121 gagacaggga gagggtcgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcgacctggc cctggactga cgttgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011222
VERSION     KHUT01011222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           57 c           87 g           44 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggatccg
       61 acagtgcttt gtgaaatacc accgctcaac ggtggaacag cttggcatac tacggatctt
      121 gagcccgaca ggggtcgtcg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgccaat ggtgaagaca gacgactggg tcggcgctga cgctcaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011223
VERSION     KHUT01011223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggtcata
       61 caagtcagag gtgaaatgcc ggggcttaac cccggaactg cctttgaaac tgtatgactg
      121 gaatcttgga gggggtagtg gaattcctag tgtagaggtg aaattcgtag atactaggag
      181 gaacaccggt ggcgaaggcg gctacctgga caagcattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011224
VERSION     KHUT01011224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           41 c           79 g           55 t
ORIGIN      
        1 gacggaggat gcaagtgtta tccggaatca ctgggcgtaa agcgtctgta ggtggtttaa
       61 aaagtctaag gttaaaaatt gaagctcaac ttcaaaggtg ccctagaaac ttttaaactt
      121 gaggatggta ggggcaaagg gaatttccag tggagcggtg aaatgcgtag atcttggaaa
      181 gaccgccaat ggcgaaggca ctttgctggg ccattactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01011225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011225
VERSION     KHUT01011225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           93 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcctt
       61 caagtcagat gtgaaagccc agggctcaac cttggaagtg catctgatac tgtgggactt
      121 gaatcctgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cagagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011226
VERSION     KHUT01011226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           93 g           41 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggtttgc
       61 taagatagag gtgaaatccc ctggctcaac cagggcaggg cctctatgac tggcaagctt
      121 gagtagggca gagggaggcg gaatgccacg tgtagcagtg aaatgcgtag agatgtggcg
      181 gaacaccgat ggcgaaggca gcctcctggg cccatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011227
VERSION     KHUT01011227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           83 g           51 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggccttg
       61 taagttaggt gtgaaatccc acagctcaac tgtggaactg cgcttaaaac tacatggcta
      121 gagggatata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggc ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011228
VERSION     KHUT01011228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcctga
       61 tcagtcgggg gtgaaagcct gtggctcaac cacagaatgg ccttcgatac tgtcgggctc
      121 gagaccggaa gaggtcagcg gaactgcgag tggagaggtg aaattcgtag agattcgcaa
      181 gaacaccagt ggcgaaggcg gctgactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011229
VERSION     KHUT01011229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           89 g           56 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggattga ctgggcgtaa agagcacgta ggcgggcttg
       61 taagtcgatt gtgaaatccc tgggcttaac ctaggaactg cagtcgaaac tgtgagtctt
      121 gaatgtttga gaggttggtg gaattccagg tgtagaagtg aaattcgtag atatctggag
      181 gaataccgga ggcgaaggcg gccaactggt gaaacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011230
VERSION     KHUT01011230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtttgt
       61 taagtcaggt gtgaaatccc ccggctcaac cggggaacag cgcttgaaac tggcagactg
      121 gagtgaggca ggggtgtgcg gaacttccag tggagcggtg aaatgtgtag atattggaag
      181 gaacgccggc ggcgaaagcg gcacactggg ccttgtctga cgctgaggca cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01011231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011231
VERSION     KHUT01011231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccccg
       61 taagtgggat gtgaaagctc ctggctcaac cagggaattg cgtcccatac tgcagggctt
      121 gagtacagga gaggatgggg gaattcccgg tgtagcggtg aaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggt ctgttactga cgctgaggcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011232
VERSION     KHUT01011232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c          102 g           39 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggcggtt
       61 caagtggctc gtgaaagacc ccggctcaac cggggagggt cgggtcagac tgaacggctg
      121 gaggatgtca gagggacacg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgcaggcg gtgtcctggg acgtacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011233
VERSION     KHUT01011233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          102 g           41 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggcgctt
       61 caagttcgtc gtgaaatact ccggctcaac cgggggaggt cgagggagac tgaggcgcta
      121 gaggacggta gagggacagg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg ctgtcctggg ccgttgctga cgctgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011234
VERSION     KHUT01011234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcctgg
       61 taggtcggtt gtgaaaactg gaggcttaac cttcagacgt cgaccgaaac cacctggcta
      121 gagtccggaa gaggagaatg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaagcg gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011235
VERSION     KHUT01011235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggctttg
       61 caagttagag gtgaaagccc ggggctcaac tccggaactg cctttaaaac tgcgatgctt
      121 gaatctggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011236
VERSION     KHUT01011236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagtcaaat gtgaaatccc agggctcaac cttggacgtg cattcgaaac tgcttaactt
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cgatgattga cgctgaggcg cgagagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011237
VERSION     KHUT01011237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           97 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctgcg
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagtg ccttggatac tgtgcagctc
      121 gagcgaggta ggggtcgtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cacgactgga cctctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011238
VERSION     KHUT01011238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           94 g           42 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggctgac
       61 caagtcctgc gtgaaatctt gcggctcaac cgcaagcggt cgtgggaaac tggtcggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctgga acgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011239
VERSION     KHUT01011239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           97 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtgata
       61 tgagtgtggt gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac gatatcactg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011240
VERSION     KHUT01011240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggcttct
       61 taagtctgat gtgaaatctc gcggctcaac cgtgagcggt cattggaaac tggggagctt
      121 gagtacagaa gaggagagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011241
VERSION     KHUT01011241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggagtgt
       61 taagtcgggg gtgaaatccc ggggcccaac cccggaactg ccttcgatac tggcgctctc
      121 gagtccggaa gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011242
VERSION     KHUT01011242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           82 g           53 t
ORIGIN      
        1 tacatagacc tcgagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtcccga
       61 taagtctctg gtgaaatccc ttggcccaac tgaggaactg tcagagatac tatcgggatt
      121 gagtatggca gaggcagacg gaattcccgg tgtagcggta aaatgcgttg atatcgggaa
      181 gaacaccaat ggcgaaggca gtctgctggg ccattactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011243
VERSION     KHUT01011243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           79 g           56 t
ORIGIN      
        1 tacgtagggt acaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggatata
       61 taagtcaggt gtgaaatacc aaagctcaac tttggtgctg catttgaaac tataaatcta
      121 gaggattgac ggagaaagcg gaattccacg tgtagcggtg aaatgcgtag atacgtggag
      181 gaacaccagt ggcgaaagcg gctttctagt ttttatctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01011244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011244
VERSION     KHUT01011244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          106 g           33 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggtcggc
       61 aaggtgccgt gtgacaatcg ggagcttaac tcccggaggc cagggtagac cggtcgactg
      121 gaggcaggga gagggaggtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcctcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011245
VERSION     KHUT01011245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           84 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgta ggcgggttgg
       61 ttagtcagtg gtgaaaaccc cgagcttaac ttgggaattg ccattgatac tgccagtctt
      121 gagtgcggtt gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaataccgat tgcgaaggca gctcactaag ccgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011246
VERSION     KHUT01011246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           79 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggatggt
       61 taagtaggaa gtgaaaggtt ggagctcaac tccaacattg ctccctatac tggctatctt
      121 gagtagcgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cgcaaactga cgctcatgtt cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01011247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011247
VERSION     KHUT01011247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           81 g           58 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtaa agagtgcgta ggcggtttaa
       61 caagtcagga gtgaaagccc aaggctcaac cttggaattg cttttgaaac tgttttactt
      121 gaatccggta gaggttggcg gaattctcag tgtagaggtg aaattcgcag atattgagag
      181 gaacaccagt ggcgtaagcg gccaactgga ccggtattga cgctgaggca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011248
VERSION     KHUT01011248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           97 g           41 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattca ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtccgtt gtgaaaaccc gaggcttaac ctcgggacgc cgacggaaac catctggcta
      121 gggtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtaccga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011249
VERSION     KHUT01011249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          111 g           34 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 cgagtcggtg gtgaaagccc ggggctcaac cccgggtcgg ccaccggtac tgccgggctg
      121 gagcgtcgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cgatggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011250
VERSION     KHUT01011250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           76 g           61 t
ORIGIN      
        1 tacatggagt ccaaacgtta tccggattta ctgggcgtaa agcgttcgta ggcgtccaac
       61 caagtcttta gtgaaatctc cgggcttaac tcggaaactg ttaaggaaac tggttggatt
      121 gagtacaaca gaggtagatg gaattcctag tgtagaggta aaattcgttg atattaggaa
      181 gaacaccagt ggcgaaggcg atctactggg ttgtcactga cgctgaggaa cgaaagtgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011251
VERSION     KHUT01011251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           42 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatca ctgggcgtaa agggcgtcta ggcggctcag
       61 taagtcttgt gtgaaatctc ccggctcaac tgggagggtg catgggaaac tgcggagcta
      121 gagacttgga ggggggtgcg gaattcccgg tggagcggtg aaatgcgttg atatcgggag
      181 gaacacctga ggcgaaggcg gcaccctggc caaggtctga cgctgaagcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011252
VERSION     KHUT01011252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggcatgg
       61 ccagtttgat gtgaaatctc cgggctcaac ccggaaatgg cattgaatac tgctgtgctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011253
VERSION     KHUT01011253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tgggagtctt
      121 gagtgtggta gaggttggtg gaacagcaag tgtagaggtg aaattcgtag atatttgcta
      181 gaacaccagt ggcgaaggcg gccaactgga ccataactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011254
VERSION     KHUT01011254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgaggggg gcaaatgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggtttc
       61 taagtcaggt gtttaatccc ggggcccaac cccggacagg cacttgaaac tgggaatctt
      121 gaatagggga gaggtcagtg gaattggagg tgtagcggtg gaatgcgtag agatctccaa
      181 gaacaccagt tgcgaaggcg gctgactgga cccatattga cgctgaggct cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011255
VERSION     KHUT01011255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           61 c          108 g           38 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattca ttgggcgtaa agcgcgcgca ggcggatgca
       61 cgcgtcccgc gtgaaatcgc ccggctcaac cgggcggggt cgcgggatac ggtgcgtctg
      121 gagcacggga gggggcagtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actgcctgga ccgtggctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01011256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011256
VERSION     KHUT01011256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           95 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatc
       61 caggtccgtg gtgaaatccc ccagctcaac tggggaactg cctcggaaac cgggtgcctc
      121 gagtggtgta ggggtgtgtg gaacggtcgg tggagcggtg aaatgcgttg atatcgaccg
      181 gaactccggt ggcgaaggcg acacactgga catctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011257
VERSION     KHUT01011257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           58 c          104 g           39 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttctg
       61 taagcgtgcg gtgaaatcgc ggggctcaac cccgcgccgg ccgtgcgaac tgcgggactc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011258
VERSION     KHUT01011258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           90 g           45 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgccggtctt
      121 gagcgactta ggggcagacg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gtctgctggg agtccgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011259
VERSION     KHUT01011259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 taagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tacatggcta
      121 gagtaccgga gaggatggtg gaattcccga agtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg accatctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011260
VERSION     KHUT01011260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggactta ctgggcgtaa agcgcgcgca ggtggatcag
       61 tatgtgccgt gtgaaagcgc catccttacg atggcgaggt cgtggtagac agctgatctt
      121 gaggctcaga gaggtcgatg gaattccggg tggagcggtg aaatgcgtag atatccggag
      181 gaacaccagg ggcgaaggcg gtcgactggc tgagttctga cactgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011261
VERSION     KHUT01011261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           91 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccacg
       61 taggtcggat gtgaaatccc tcggctcaac cgaggacgtg cattcgaaac cccgtggctc
      121 gagtcctgga gagggtagtg gaattctcgg cgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggc ggcgaaagcg gctacctgga cagagactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011262
VERSION     KHUT01011262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           42 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaacgg ctgggcatac tggcgatctt
      121 gaggtgcgta ggggcgagcg gaacgaacgg tggagcggtg aaatgcgtag atatcgttcg
      181 gaacgccgac ggtgaaaaca gctcgctggg cgcatcctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011263
VERSION     KHUT01011263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c          100 g           37 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggcctga
       61 taagtcggga gtgaaattcc ggggctcaac cccggagctg ctttcgaaac tgccaggcta
      121 gggaccggta gaggtcggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccgactggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011264
VERSION     KHUT01011264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           80 g           57 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggggata
       61 taagtcagat gtgaaatcct ggggctcaac ctcagaactg catttgaaac tgtatctctt
      121 gagggtagaa ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatt ttattcctga cgctgatgcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01011265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011265
VERSION     KHUT01011265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           96 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttcgc
       61 taagtcgaat gtgaaaactc tgggcttaac ccagagcctg cattcgatac tggcgggcta
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagagtctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011266
VERSION     KHUT01011266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          101 g           45 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgtcg
       61 taagtttgtg gtgaaagcct ggggctcaac cccaggtctg ccgcgaatac tgcgaggctt
      121 gagtactgta gaggcaggta gaatatcggg tgtagcggtg gaatgcgtag agatccgata
      181 gaagaccggt ggcgaaggcg gcctgctggg cagtaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011267
VERSION     KHUT01011267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggactta ctgggcgtaa agggtgcgta ggcggggata
       61 taagttagtt gtgaaagacc tgggcttaac cgagggatag caattaagac tgtatttcta
      121 gagttgagta gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt tgcgaaggcg gctctctgga ctcaaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011268
VERSION     KHUT01011268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggtatgg
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaattg cgctctaaac tgccgtactt
      121 gaggaagcta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg gcttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011269
VERSION     KHUT01011269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           85 g           52 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggatgtctt
      121 gggccactta ggggtcactg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaaa ggtgaaaaca ggtgactggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011270
VERSION     KHUT01011270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           45 c           98 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggttctt
       61 taagtctggt gtgaaaggtt tgggctcaac ccgaacagtg cactggagac tgggggactt
      121 gagttaggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca gctctctggt cctatactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011271
VERSION     KHUT01011271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggaatta ctgggcttaa agggtgcgta ggcggctctg
       61 taagtgcttt gtgaaatacc acggcctaac cgtggaattg ctcggcagac tgcagggctt
      121 gaggtcggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011272
VERSION     KHUT01011272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           98 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctg
       61 ccggtcgggg gtgaaatacc acagctcaac tgtggaagtg cccccgatac cgcgggcctc
      121 gagggggata ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acacgctgga tccctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011273
VERSION     KHUT01011273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 gacgaactgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccgg
       61 taagtcaggg gtgaaatccc acagcttaac tgtggaagtg cctttgatac tgccggtctg
      121 gagggagata ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011274
VERSION     KHUT01011274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           88 g           51 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctta
       61 tttgtcttat gtgaaagcct tgagcttaac tcaagaagtg cgtaagaaac ggtaagacta
      121 gagtcccaga gaggcttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcaagctgga tgggcactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011275
VERSION     KHUT01011275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           85 g           52 t
ORIGIN      
        1 tacgtaggtc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcggctagt
       61 taagtcttcg gtgaaatctc cgggcccaac tcggaaactg tcgaagatac tggctggctg
      121 gagtatggca gaggcagatg gaattgatgg tgtagcggta aaatgcgtag atatcatcaa
      181 gaacacccat ggcgaaggca gtctgctggg ccactactga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011276
VERSION     KHUT01011276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgct gtgaaaaccc agggctcaac cctgggcgtg cagtggatac tgccgggcta
      121 gagggtggta gaggcgagtg gaattcccag tgtagcggtg aaatgcgcag atattgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccatacctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01011277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011277
VERSION     KHUT01011277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccggt
       61 tgcgtcggat gtgaaagccc tgggctcaac ccgggaatgg cattcgatac gggctggctt
      121 gaagtcggaa gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgat ggcgaaggca gctctctggt ccgaccttga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011278
VERSION     KHUT01011278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           89 g           48 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgaacgta ggcggcttag
       61 taagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tgctaggctt
      121 gaatgtggga gaggaaagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccaacattga cgctgaagtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011279
VERSION     KHUT01011279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          103 g           38 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggttgtcgtg
       61 tgcgtcgatg ctgaaagcgc cgggcttaac ccggcgaggt gcagcgatac gacacgactg
      121 gaggcaggga gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgccagt gacgaaggtg gccacctggc cctgacctga cactgaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011280
VERSION     KHUT01011280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           80 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggtctca
       61 taagtcagtg gtgaaagcct acagctcaac tgtagaactg ccattgatac tgtgggactt
      121 gaatctggtc gaagtgggcg gaatgtgaca tgtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat agcgaaggca gctcactagg tcagtattga cgctcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011281
VERSION     KHUT01011281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggacgtt
       61 taagtcagag gtgaaagccc aaggctcaac cttggaatgg cctttgatac tggacgtctt
      121 gagaccggaa gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011282
VERSION     KHUT01011282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           74 g           59 t
ORIGIN      
        1 tacaggggat gcgagtgtta tccggaatca ttgggcgtaa agcgtctgca ggttgatatc
       61 taagtccttt gttaaatctt caggcttaac ctgaaatctg caaaagaaac tggttatctt
      121 gagtatagaa gaggtaaagg gaatttccag tggagcggtg aaatgcgtag agattggaaa
      181 gaacaccaag ggcgaaggca ctttactggg ctattactga cactcagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011283
VERSION     KHUT01011283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           96 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggatggc
       61 caagtcaagg gtgaaagccc ccggcccaac cggggaagtg cctctgatac tggtcatctc
      121 gagcgggata ggggcagctg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga tccctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011284
VERSION     KHUT01011284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           96 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggagtaa ctgggcgtaa agcgcacgca ggtggtcgtg
       61 tgcgtggggt gtgaaagctc tgggcttaac tcagagaggc cacctcatac ggcacggctc
      121 gagccctgca gaggtccacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg caggcgctga cactcatgtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011285
VERSION     KHUT01011285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          103 g           43 t
ORIGIN      
        1 tacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtcggct gtgaaatccc ggggctcaac cccgggctgg cagtcgatac gggcaggctg
      121 gagtccggca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccggtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011286
VERSION     KHUT01011286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          107 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggatgct
       61 taagtcgtca gtgaaagccc ccggctcaac cggggagggt ctggcgagac tgggcgtctg
      121 gaggacggaa gaggagagtg gaattgctgg tggagcggtg gaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gctctctggt cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011287
VERSION     KHUT01011287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggtcaac
       61 caagtcagat gtgaaagccc cgggctcaac ctgggaactg catttgagac tggatgactt
      121 gagttcggga gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011288
VERSION     KHUT01011288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           86 g           53 t
ORIGIN      
        1 tacagaggtc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 ttagtacctt gtgaaatccc acggcttaac cgtggaatgg ctgggtatac tggcgatctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011289
VERSION     KHUT01011289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           81 g           56 t
ORIGIN      
        1 tacgaaggga gctagcgtta ctcggaatca ttgggcgtaa agcgtgcgaa ggcggttttt
       61 caagtcagaa gtgaaagcct gaggctcaac ctcagaattg cttttgaaac tgttgaacta
      121 gagtatcaga ggggatagcg gaattcctag tgtagaagtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctggc tgaatactga cgctgttgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011290
VERSION     KHUT01011290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           42 c           93 g           55 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggtggttcac
       61 taagtttggt gtaaaagacg ggggctcaac cctcggatcg cactgaatac tggtggactg
      121 gagtggagta gaggcaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaagcg gcttgctggg ctttaactga cgctgaggaa cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01011291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011291
VERSION     KHUT01011291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           85 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggtgcgta ggtggatatt
       61 taagtcagat gtgaaacccc tgggctcaac ccagggactg catttgaaac tggatatctg
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011292
VERSION     KHUT01011292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggggta
       61 caagtcagat gtgaaatcct ggggctcaac tccagagcgg catctgaaac tatacttctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011293
VERSION     KHUT01011293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           53 t
ORIGIN      
        1 tacgtagacc tcaagcgtta ttcggattta ttgggcgtaa agcgcacgta ggtggttttg
       61 tgcgtcttct gttaaagccc accgcccaac ggtggaagtg caggagatac gacaagacta
      121 gaggaggtta gaggtgcatg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaacaccgaa ggcgaaggca atgcactggg accttcctga cactgagttg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01011294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011294
VERSION     KHUT01011294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           98 g           41 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcacct gtgaaacccc cgggctcaac tcggggcctg caggcgaaac tgccgtgcta
      121 gagtgcggaa gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011295
VERSION     KHUT01011295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtaa agggtacgta ggcggttttg
       61 caggtggggt gtgacatctc cgagcttaac tcggaagggc cgctccaaac cgtggaactt
      121 gagccgtgca gatgtcggtg gaattgccag tgtagcagtg aaatgcgtag agattggcag
      181 gaacaccaag ggcgcaagca gccgacaggg cacgagctga cgctgaggta cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011296
VERSION     KHUT01011296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggtctgt
       61 caagttcgag gtgacagtca ccagcttaac tggtggagta ccttgaaaac tggcagactt
      121 gagggccaga gagggacacg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca gtgtcctggc tggtacctga cgctcaagtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011297
VERSION     KHUT01011297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agagcccgcg ggcggttgct
       61 caggtcggtt gtgaaatctc ccggctcaac tgggagggga ctgccgaaac cgagtgactt
      121 gagggccgga gagggacatg gaattcccgg tgtagtggta aaatgcgttg agatcgggag
      181 gaacaccagt ggcgaaagcg atgtcctggc cggaacctga cgcttagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011298
VERSION     KHUT01011298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           87 g           52 t
ORIGIN      
        1 tacggaggag gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtgggtgac
       61 caagtcagtt gttaaaacgt gaagcttaac ttcatagagg cgactgaaac tggtaatcta
      121 gagagtggta ggggtagagg gaatttccag tgtagcggtg aaatgcgtag atattggaaa
      181 gaacaccggt ggcgaaagcg ctctactggg ccattactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01011299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011299
VERSION     KHUT01011299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           88 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcgggttat
       61 taagtcagat gtgaaattct tgggctcaac ccaagcgctg catctgaaac tgatgatctt
      121 gagtactgga gagggaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011300
VERSION     KHUT01011300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttttg
       61 taagccagct gtgaaatccc cgggcttaac ctgggaatgg cggttgggac tacaagacta
      121 gagtgcggca gaggggggtg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaggca gccccctggg tcgacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011301
VERSION     KHUT01011301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           91 g           44 t
ORIGIN      
        1 tacgaactgc gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggtgcgg
       61 aaagtagggt gtgaaatgcc ttggctcaac caaggcactg cgctctaaac tcccgtgctc
      121 gagtgagtca ggggtacgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccggc ggcgaaagcg gcgtactggg gctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011302
VERSION     KHUT01011302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtctggg gtgaaatcct taagcttaac ttaagaagtg cctcagaaac ggtgagactg
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caggaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011303
VERSION     KHUT01011303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggattta ctgggtgtaa agggcacgta ggcggtccga
       61 taagccccgt ttgaaatgcc tgggcttaac ctgggcacgt ggcgggggac tgacggactt
      121 gaggatggca gaggtaggcg gaattcccgg tgtaagggtg aaatctgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gcctactggg ccatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011304
VERSION     KHUT01011304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           67 c          101 g           32 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgca ggcggccgcg
       61 cacgtgtggg gtgaaagctc ggggctcaac ccagagtcgc ccccgcatac ggcacggctc
      121 gagcccggga gaggtccctc gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaagacccaa ggggaagcca ggggactggc ccggtgctga cgctcaggtg cgatagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011305
VERSION     KHUT01011305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggcttga
       61 taagttgggt gtgaaatccc cgggcttaac ctgggaattg cattcaagac tgtcttgcta
      121 gagtgtggaa gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gcttcctggt ccaacactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011306
VERSION     KHUT01011306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           96 g           39 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggccagg
       61 taagttggct gtgaaatctc ccggctcaac tgggagggga cggtcaagac tgcctggctg
      121 gaggcaggga gagggaggcg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaagcg gcctcctaga cctgacctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011307
VERSION     KHUT01011307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattca ctgggcgtaa agcgcgcgta ggcggacatt
       61 taagttgtgg gtgaaatccc ggggctcaac cccggaactg cctgcaaaac tgggtgtctt
      121 gaggtatgaa gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gcttcctggt catatcctga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011308
VERSION     KHUT01011308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           77 g           67 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattta ttgggtttaa agggtgcgta ggtggctctg
       61 taagtcagtt gtgaaagttt gcggctcaac cgtaaaattg caattgaaac tgtagagctt
      121 gagtgtattt gaggtgggcg gaattcgtag tgtagcggtg aaatgcttag atattacgaa
      181 gaactccgat tgcgtaggca gctcactaaa ctacaactga cactgaggca cgaaagtgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01011309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011309
VERSION     KHUT01011309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggtggcatat
       61 caagtctggt gttaaagtcc ggagctcaac tccgggagag ccctggatac tgataagctg
      121 gagtgcagaa gaggcaagag gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggc ggcgtaagcg tcttgctggt ctgttactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01011310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011310
VERSION     KHUT01011310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           83 g           48 t
ORIGIN      
        1 gacggaaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgtccgca gctgtctttg
       61 taagtctggt ttaaaagcac gaagctcaac tccgtgtatg tgccggaaac tgcaaagatt
      121 gagtcaccca gaggcaactg gaatgccgtg tgtaggggta aaatccgttg atccacggta
      181 gaacgccaaa agcgaaggca ggttgctggg ggtgcactga cagtcatgga cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011311
VERSION     KHUT01011311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctctgatac tggcgggctg
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011312
VERSION     KHUT01011312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           62 c          111 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtcgtc
       61 taagcccacc gtgaaagccc ccggctcaac cggggagggt cggtggggac tgggcgactt
      121 gaggacggca ggggctggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccagctggg ccggtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011313
VERSION     KHUT01011313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggtggttacc
       61 caagtcgggt gtgaaatccc ctggctcaac cagggaagtg catccgaaac taggtagcta
      121 gaggacggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctggg tcgttcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011314
VERSION     KHUT01011314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           97 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgaga
       61 tgagtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gatcttgctg
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011315
VERSION     KHUT01011315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatca ctgggcgtaa agcgtacgca ggctgcttgt
       61 taagttaggg gtgaaatccc agggctcaac cctggaactg cctttaatac tggcaagcta
      121 gagtccgtga gaggagtgtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acactctggc gcggtactga cgctcatgta cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011316
VERSION     KHUT01011316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcgggccac
       61 caagtctgtg gtgaaatcct ccggctcaac cggagaactg ccgtggatac tgggggcctc
      121 gagggagata ggggcgtgcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaactccggt ggcgaaggcg gcacgctgga tctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011317
VERSION     KHUT01011317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           85 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatatt
       61 taagtcggaa gtgaaagccc tgggcttaac ctgggaattg cctccgatac tggatgtcta
      121 gagtatggta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctatctgga ccaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011318
VERSION     KHUT01011318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggtcagc
       61 aaagttggat gtgaaatccc tcggctcaac cgaggaagtg cattcaaaac taactggctg
      121 gagagtggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccgga ggcgaaggcg gccttctgga ccatttctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011319
VERSION     KHUT01011319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           99 g           46 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgt
       61 taagttcggt gtgaaatccc tcggctcaac cgaggaagtg cgccggatac tggcaggctt
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011320
VERSION     KHUT01011320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacgtaggga gcaagcgtta tccggattta ctgggtgtaa agggtgcgta ggcggttttt
       61 taagtcgaaa gtgaaatttc ggggctcaac cccggagctg ctttcgaaac tggagaacta
      121 gagtgtggga gaggaaagtg gaactttgag tgtagcggtg aaatgcgtag agattcaaag
      181 gaacaccagt agcgaaggcg gctttctgga ccattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011321
VERSION     KHUT01011321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           84 g           56 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtttgg
       61 taagtctgat attaaagacc ggagctcaac ttcgggactg tatcggaaac tatcagacta
      121 gaggacaata gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg gacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011322
VERSION     KHUT01011322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           78 g           60 t
ORIGIN      
        1 tacggaggtg gcgagcgtta ctcggaatta ttgggtgtaa agggcaagta ggcggcttaa
       61 taagtgaggg gtgaaatcct gtgactcaat cgcagaattg cctttcaaac tgttgagctt
      121 gagtctgaaa gagggaaatg gaattagcag tgtaagggtg aaatctgtag atattgctaa
      181 gaacaccgac ggcgaaggca atttcctggt tcagtactga cgctaaattg cgaaagctag
      241 ggtagcaaac gga
//
LOCUS       KHUT01011323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011323
VERSION     KHUT01011323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           60 c           93 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggccac
       61 caagtcaggg gtgaaatccc acggctcaac cgtggaagtg cctctgatac ttgtggcctc
      121 gaggggtgca ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctaga caccttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011324
VERSION     KHUT01011324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttttg
       61 taagtcagtg gtgaaatctc agagctcaac tctgaatgtg ccaatgatac tgcaagactt
      121 gagagcagtc gaggtagctg gaatgtgtag tgtagcggtg aaatgcatag agattacaca
      181 gaacaccaat tgcgaaggca agttactaga ctgtttcaga cgctgatgca cgaaagtgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011325
VERSION     KHUT01011325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           38 c           84 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggttta
       61 taagtcagtg gtgaaagttt gcagcttaac tgtaaaattg ccattgatac tgtagatctt
      121 gagtgcagtc gaggtggttg gaatgcgtag tgtagcggtg aaatgcttag atattacgca
      181 gaacacctat agcgaaggca gatcgccagg ctgtaactga cgctgaggca cgaaagtgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011326
VERSION     KHUT01011326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacagggggt gcgagcgtta ttcggagtta ctgggcgtaa agcgtgcgta ggcggtttag
       61 caagtctggt gtgaaagccc tggactcaat tcaggaagtg cattggatac tgctagacta
      121 gagtgtagga ggggagagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacgga agcgaaggcg gctctctaga ctacaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011327
VERSION     KHUT01011327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaaggt
       61 taagcgtggc ttgaaagccc ccggctcaac cggggagggc ggctgcgaac tggccttctt
      121 gaggtcgata gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg tcggacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011328
VERSION     KHUT01011328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           88 g           54 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttttc
       61 taagttagag gtgaaagcct ggagctcaac tccagaattg cctttaagac tggatcactt
      121 gaatcatgga gaggcgggtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcccgctgga catgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011329
VERSION     KHUT01011329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 aacgtaggtc acaagcgttg tccggaatta ctgggtgtaa agggagcgca ggcgggaaga
       61 caagttggaa gtgaaatcca tgggctcaac ccatgaactg ctttcaaaac tgtttttctt
      121 gagtagtgca gaggtaggcg gaattcccgg tgtagcggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcctactggg caccaactga cgctgaggct cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011330
VERSION     KHUT01011330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           97 g           51 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agggctcgca ggcggcttct
       61 taagtctgac gtgaaatctc ccggcttaac tgggagggtg cattggatac tgggaagctt
      121 gagggcatca gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg gtgcatctga cgctgaggag cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011331
VERSION     KHUT01011331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           91 g           50 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ctgagaatca ctgggtttaa agggtgcgta ggtggcgcgt
       61 taagtccgtt gtgaaatccc ttggctcaac caaggaaatg cttcggatac tggcgtgctt
      121 gaggccggta ggggtcactg gaactgacgg tggagcggtg aaatgcgtag atatcgtcag
      181 gaacgccaat ggtgaagaca ggtgactggg ccggttctga cactgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011332
VERSION     KHUT01011332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctcg
       61 tttgtctaag gtgaaagccc tgagcttaac tcaggaagtg ccttagaaac ggcgagacta
      121 gagtcctaga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga taggaactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011333
VERSION     KHUT01011333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           82 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgca ggcggatcgt
       61 taagtcagcg gtgaaagccc tcagctcaac tgaggaactg ccgttgatac tggcgatctt
      121 gagtgcgagt gaagttggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacacctat tgcgaaggca gctgactaac tcgaaactga cgctcaggga cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011334
VERSION     KHUT01011334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           94 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggttact
       61 taagtctggt gtgaaagccc ggggcttaac cccggaaggg cactggatac tgggtaactg
      121 gagggcgaga gaggaaagtg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatccgt ggcgaaggcg actttctggc tcgtttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011335
VERSION     KHUT01011335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c          103 g           31 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggaatta ctgggcgtac agcgcgcgca ggcggagcgc
       61 gacgtgccgt gtgacaagcc accgctcaac ggtggagcgc cacggtacac gagcactctg
      121 gaggcaggga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01011336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011336
VERSION     KHUT01011336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           90 g           46 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggtctgt
       61 caagtcgggc atgaaagctc tcggctcaac cgggagaggc tgtccgatac tggcagactt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccacctgga ctgcacctga cgctcaaaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011337
VERSION     KHUT01011337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggctga
       61 taagtcagtg gtgaaagccc caagcttaac ttgggaattg ccattgatac tgccagtctt
      121 gaatacggtt gaggtaggcg gaatatagca tgtagcggtg aaatgcttag atatgctata
      181 gaacaccgat tgcgaaggca gcttactaag ccgttattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011338
VERSION     KHUT01011338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacgaaggga tctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtttga
       61 caagtcagat gtgaaagccc tgggcttaac ccaggaactg catttgaaac tgtcaggctt
      121 gagtatggga gaggtaagta gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaataccagt ggcgaaggcg gcttactgga ccataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011339
VERSION     KHUT01011339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 tcagtccggc gtgaaacctc ggggctcaac cccgagcctg cgctggatac tgcctcgctc
      121 gagtccggga ggggagagcg gaattcccag tgtagcggtg aaatgcgcag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011340
VERSION     KHUT01011340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           73 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtaggaaag
       61 ttagtcagtg gtgaaaattc taagcctaac ttagaaattg ccattgatac tgcttttctt
      121 gagtacgatt gaagtggacg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccaat tgcgaaggca gttcactaag tcgtcactga cactgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011341
VERSION     KHUT01011341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           84 g           50 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtgtcctgc
       61 caagtctggt gtcaaatggc gatgctcaac atcgtcaaag tgccggaaac tagtaggatt
      121 gagtcactca gaggtatctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgcctaa agcgaaagca ggatactggg ggtgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011342
VERSION     KHUT01011342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           51 c           83 g           47 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtgtcatag
       61 caagtctggt gtcaaaacac gaagctcaac tccgtgtacg tgccggaaac tactgtgatt
      121 gagtcactca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgccaaa agcgaaggca ggatgctggg agtgcactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011343
VERSION     KHUT01011343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggaccat
       61 taagtcagtg gtgaaagccc tctgctcaac agaggaactg ccattgatac tgatggtctt
      121 gagtataagt gaggttggcg gaatgtgtgg tgtagcggtg aaatgcttag atatcacaca
      181 gaacaccgat tgcgaaggca gctggccaac ttataactga cgctgaggga cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011344
VERSION     KHUT01011344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccaaa
       61 cgggttaggt gtgaaagccc tcggctcaac cgaggaagtg cgcctaaaac tatttggctc
      121 gagtgcagga gaggaggacg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gttctctgga ctgcaactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011345
VERSION     KHUT01011345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           95 g           40 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggaatta ctgggcgtaa agcgcatgca ggcggttagc
       61 aaagtcggac gtgaaagctc ctggctcaac taggagaggc cgttcgagac tggccgacta
      121 gagggtgata gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctggg tcacacctga cgctcagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01011346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011346
VERSION     KHUT01011346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggttagg
       61 taagtctaat gtgaaatcat ctcgcttaac gaggtgcccg catcggaaac tgcttgactt
      121 gagtatagga gaggataacg gaattcctgg tgtagcggtg gaatgtgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gttatctggc ctaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011347
VERSION     KHUT01011347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           87 g           52 t
ORIGIN      
        1 tacagagggt gctaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggccagg
       61 taagtgtaat gtgaaatccc acggctcacc cgtggaactg ctttgcatac tgcctggctc
      121 gaggaaggta ggggagagca gaactcttgg aggagcggtg aaatgcgtag atatcaagag
      181 gaataccagt ggcgaaggcg gctctctggt ccttttctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011348
VERSION     KHUT01011348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           54 c           73 g           52 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggttcat
       61 taagtcatag gtcaaatccc ggggctcaac cccgtaactg cccatgatac tgatgaactc
      121 gaggccagta gaggcaagcg gaattacagg tgtagcggta aaatgcgttt atatctgtaa
      181 gaacaccaaa ggcgaaagca gcttgctgga atggtcctga cactcatgga cgaaagcgtg
      241 gggagcgaaa agg
//
LOCUS       KHUT01011349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011349
VERSION     KHUT01011349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtcgggg gtgaaatccc tcagctcaac tgaggaactg ccttcgatac tgggggcctc
      121 gagggaggta ggggcggccg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ggccgctgga cctcatctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011350
VERSION     KHUT01011350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttct
       61 ccagtcaggg gtgaaatccc acggcttaac cgtggaacgg cccctgatac tgagagtctc
      121 gaggggtgta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011351
VERSION     KHUT01011351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           86 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ctgggcgtaa agcgtgcgta ggcggcgagg
       61 taagtcagat gtgaaagctc ggggctcaac cccgaaaatg catccgatac tgccttgctt
      121 gagtaatgga ggggagtctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg agactctgga catttactga cgctgaagca cgaaggctag
      241 ggtagcgaag cgg
//
LOCUS       KHUT01011352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011352
VERSION     KHUT01011352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           86 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgtg
       61 tcagttaggt gtgaaattcc tgggcttaac ctgggaactg cgcctgatac tgcatagcta
      121 gagtattgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga caaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011353
VERSION     KHUT01011353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtctgc
       61 caagtcgggg gtgaaatctt tcggctcaac cggaaaagag ccttcgatac tggcaggcta
      121 gagggaggta ggggtagcgg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg cgttactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011354
VERSION     KHUT01011354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcaccc
       61 taagtaccct ctgaaatctc ccggctcaac cgggagggtg gagggtagac tagggagcta
      121 gagagaggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg cctcttctga cgccgaggcg cgaaggctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011355
VERSION     KHUT01011355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           93 g           47 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggaatta ctgggcgtaa agagcgtgta ggcggaagga
       61 caagttggtc gtgaaagctt ccggctaaac tgggagaggt cgatcaagac tgtttttcta
      121 gagttcgaca gaggggtatg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg atgccctggg tcgaaactga cgctaagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011356
VERSION     KHUT01011356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           83 g           56 t
ORIGIN      
        1 tacgaagggt gctagcgtta ttcggattta ttgggcgtaa agggtgtgta ggcggcatat
       61 caagtctatt gttaaagact ccagcctaac tggagaaatg cggtggaaac tggtgtgctt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatatctga cgctgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011357
VERSION     KHUT01011357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           87 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggctatg
       61 taagtctgct gttaaagaac ctagcttaac taggtaaaag cagtggaaac tacaaggcta
      121 gagtgcgttc ggggtagagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccagt ggcgaaggcg ctctactagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01011358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011358
VERSION     KHUT01011358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 aacgtaggtt ccgagcgtta tccggattta ctgggcgtaa agcgtgttca ggcggcttat
       61 caagtcgggc atgaaatctc tcggctcaac cgggaggggc tgtccgaaac tgaaaggctg
      121 gagggcagga gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccacctggc ctgtacctga cgctcaaaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011359
VERSION     KHUT01011359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           43 c           73 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta tccggaatca ttgggcgtaa agcgtccgca ggtggttagt
       61 taagttggat attaaagaca atagctcaac tattgcagcg tatccaaaac tgactagcta
      121 gagactcata gaggtaagtg gaattttgca tgtaggggta aaatccgtag atatgcagag
      181 gaacaccaaa agcgaaagca gcttactgga tgagttctga cactcaggga cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011360
VERSION     KHUT01011360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           89 g           52 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatga ctgggtgtaa agagcgtgga ggcggtttgt
       61 taagttagtt gttaaatcgc ccggcttaac cgggcaatcg cgattaaaac tggcaagcta
      121 gagtgtggga gagaaaggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccttttggc ccaccactga cgctggagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011361
VERSION     KHUT01011361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           81 g           60 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta gacggtttgt
       61 ttagtctgtt gttaaatctt tcggcttaac cgaaaatcag caatggaaac tggcaggcta
      121 gagtgtgaaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt tcactactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011362
VERSION     KHUT01011362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           93 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcttg
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tgcgagactt
      121 gaggcaatga ggggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc attagcctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011363
VERSION     KHUT01011363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           93 g           53 t
ORIGIN      
        1 tacggggggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggcggttttg
       61 taagtctgat gtgaaagccc tgggctcaac ccaggaagtg cattggatac tgcaagactt
      121 gagttcggga gaggacagtg gaattcctgg tgtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctgtctgga ccgatactga cgctgagatg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011364
VERSION     KHUT01011364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           84 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ttgggcgtaa agggcgcgta gacgggaagg
       61 caagtcagat gttaaagccc ggggcttaac cccggaaaag catttgaaac tgcctttctt
      121 gaggatagac ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagt ttattcctga cgttgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011365
VERSION     KHUT01011365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           87 g           48 t
ORIGIN      
        1 tacgaattgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccatg
       61 aaggtctgat gtgaaatccc acggctcaac cgtggaattg cgttggaaac cacatggctt
      121 gagtaggaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tcctaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011366
VERSION     KHUT01011366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           97 g           48 t
ORIGIN      
        1 tacgggaggt gcgagcgtta ctcggaatca ctgggcttaa agggggtgta ggcggaatgg
       61 taagtgtctt gtgaaagccc tcggctcaac cggggaattg ctgggcaaac tgccgttctt
      121 gaggcggata gaggtgggtg gaacgagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccggt ggcgaaagcg gcccgctgga tccgtcctga cgctgatacc cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011367
VERSION     KHUT01011367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c          102 g           37 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggagggt
       61 caagcggggt gtgaaagctc tcggcttaac tgagagagtg cgctccgaac tggccgacta
      121 gagggcaggc gacggcggtg gaatacccgg tggagtggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg accgccgagc ctgcacctga cgctgaagcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011368
VERSION     KHUT01011368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           94 g           51 t
ORIGIN      
        1 cacgggggga acgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcgggtcag
       61 caagtcggat gtgaaagtcc agggcttaac cctgaatctg cattcgaaac tgttggtctt
      121 gagtactgga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgtt ggcgaaggcg gctcactgga cagatactga cgctgaggca cgaaggtcgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011369
VERSION     KHUT01011369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           81 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaaagg
       61 aaagtcggat gtgaaatccc gggactcaac cccggaactg cattcgaaac ttcctttcta
      121 gagggtagac ggagaaaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg attttctagt ttacacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011370
VERSION     KHUT01011370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           85 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgct gtgaaagctc ctggctcaac tgggagaggc cagcagatac tgtcaggctt
      121 gagtatgaga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccgat ggcgaaagca ggtacctgga ccattactga cactaagctg cgaaagctaa
      241 gggagcaaac agg
//
LOCUS       KHUT01011371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011371
VERSION     KHUT01011371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctgg
       61 caagtcggtt gtgaaatccc cgggctcaac ccgggaactg catccgaaac tgctgggcta
      121 gagttccgga gaggaaggca gaattcccaa tgtaggggtg aaatccgtag atattgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011372             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011372
VERSION     KHUT01011372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          100 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggtcgc
       61 tgcgtcggct gctgaaatcc cccggctcaa ccggggaacg ggcaccgata cgagcggcct
      121 ggaggggggt aggggggtct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011373
VERSION     KHUT01011373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggcggctttg
       61 caagtcaggg gtgaaatccc acagctcaac tgtggaattg cctctgaaac tgcatggctg
      121 gagtattgga ggggtcaggg gaattcctaa tgtagtggtg aaatacgtag atattaggag
      181 gaacaccggt ggcgaaggcg cctgactgga caataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011374
VERSION     KHUT01011374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           96 g           48 t
ORIGIN      
        1 tacgtagggg ccaagcgtta cccggattta ctgggcgtaa agggcgtgta ggtggctgcg
       61 taagtttgct gtgaaatctt tcggctcaac cgggagaggt cagcgaatac tgcgcggctt
      121 gagggcagca gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg ctgtccctga cactgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011375
VERSION     KHUT01011375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtcttt
       61 taagtcagtg gtgaaatcct gcagctcaac tgtagaacag ccattgatac tgaaagactt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacccctat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011376
VERSION     KHUT01011376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgcgta ggcggccatt
       61 taagttgaat gtgaaatccc cgagctcaac ttgggaagtg cattcaaaac tggatggctt
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcagt ggcgaaggcg accacctgga cgaagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011377
VERSION     KHUT01011377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           46 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 tgcgtcaggt gtgaaagccc cgggctcaac ctgggatgtg cacttgatac gggcgggctg
      121 gaatccggga gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccgat ggcgaaggca gccatctgga ccggtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011378
VERSION     KHUT01011378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcccat
       61 tatgtcaggt gtgaaagccc tcggcttaac cgaggaagtg cgcctgaaac tgataggctt
      121 gagtacgaga gaggaggacg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggc ggcgaaagcg gttctctgga tcgatactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011379
VERSION     KHUT01011379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggattta
       61 taagtgagtt gtgaaatccc tgggcttaac ctaggaattg caacttagac tgtagatctg
      121 gagtgcagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacacccgt ggcgaaggcg gccacctgga ctgagactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011380
VERSION     KHUT01011380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           86 g           50 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagtacgta ggcggtcaag
       61 taagacacgg gttaaatctc cgctctcaaa gcggaaagtg cccgcgtgac tgctcgactc
      121 gtcggtagca ggggcaagcg gaattgtcgg tgtaggagtg aaatccgttg atatcgacaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctatttgaga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011381
VERSION     KHUT01011381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggctggg
       61 taagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac tgccctgcta
      121 gtgtgcagtt agggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctgttacaga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011382
VERSION     KHUT01011382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           98 g           35 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcaagca ggcggtagcg
       61 caagtggggc gtgaaagctc tcggctaaac cgggagaggc cgttccaaac tgcgcaactg
      121 gagcacagca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgtgactga cgctgagatg cgacagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011383
VERSION     KHUT01011383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcacgg
       61 caagtcgggt gtgaaatcct gcagcttaac tgtagaattg cattcgaaac tgccgagcta
      121 gaggactgga gaggagatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg gatctctgga caggccctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011384
VERSION     KHUT01011384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          101 g           39 t
ORIGIN      
        1 tacggagggg gcaagcgttg tccggactta ctgggcgtaa agggtgcgta ggcggttggg
       61 tgcgttcgcc ctgaaagcac cgggctcaac ccggtgaggc gggcgaatac gacccgactg
      121 gaggctcaga gagggtggcg gaactcctgg tgtagtggtg atatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc tgagtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011385
VERSION     KHUT01011385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           83 g           56 t
ORIGIN      
        1 gacgagtctg acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtatgg
       61 taagtcgggt gtgaaatccc ttagctcaac taaggaactg cacccgatac tattatgctt
      121 gagtaagaca gaggtgagtg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacaccggt ggcgaaggcg actcactggg tctttactga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011386
VERSION     KHUT01011386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c          100 g           39 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggacggt
       61 caagcgcggt gtgaaagctc ctggctcaac tgggagagtg cgctgcgaac tgatcgacta
      121 gagggcaggc gacggcggtg gaattcccgg tggagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgccgagc ctgttactga cgctgaagcg cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011387
VERSION     KHUT01011387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           86 g           49 t
ORIGIN      
        1 tacggaggtt ccaagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggtctgg
       61 tcagtctaaa gtggaagccc ggggcttaac ctcggaaagc ctttggatac tgccagactt
      121 gagtacgtga gaggaaggcg gaattcaagg tgtagcggtg aaatgcttag atatcttgag
      181 gaacaccaat ggcgaaagca gccttctggc acgaaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011388
VERSION     KHUT01011388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 cacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggctttt
       61 caagttggat gtgaaagctc ccggcttaac tgggagaggt cattcaaaac tggggagcta
      121 gaggatggaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tcacacctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011389
VERSION     KHUT01011389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           87 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 caagttgggt gtgaaatccc tcggcttaac cgaggaacgg cgcccaaaac tgccgtgctc
      121 gagggagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgatgca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011390
VERSION     KHUT01011390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggattta ctgggcgtaa agcgtacgta ggcggatgat
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tggtcgtctt
      121 gagtccggga gaggtgagtg gaactcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011391
VERSION     KHUT01011391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgtta ttcggattaa ctgggcgtaa agagcgtgtc ggcggtttga
       61 taagtcagct gtaaaagaac tcggcttaac cgggtaaatg cggatgaaac tgtcagacta
      121 gagtgcggga gagaaaggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccttttggc ccgtaactga cgcttaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011392
VERSION     KHUT01011392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           84 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttctt
       61 taagtcagcg gtgaaatatg tcagcttaac tggcaaggtg ccattgatac tggagaactt
      121 gagtactgat gaggtaggcg gaatggacgg tgtagcggtg aaatgcttag atatcgtcca
      181 gaacaccgat agcgaaggca gcttactaag gagtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011393
VERSION     KHUT01011393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           87 g           49 t
ORIGIN      
        1 gacggaccat ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc ttggcttaac caaggaatgg cgctcaaaac taccgtgctt
      121 gaggaagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tcttttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011394
VERSION     KHUT01011394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           83 g           49 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggccctg
       61 aaagtaaggt gtgaaatccc acagctcaac tgtggaactg cgctttaaac tacagggcta
      121 gagggataca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gcttactggg tatcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011395
VERSION     KHUT01011395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcgcgta ggcggatctt
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgatac tggggatctt
      121 gagttcgaga gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggc tcgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011396
VERSION     KHUT01011396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           89 g           51 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcgggaagc
       61 caagtcaggg gtgaaatctc acggctcaac cgtgaaactg cctttgatac tggttttctt
      121 gagtcaggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc cctgaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011397
VERSION     KHUT01011397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           60 c          111 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgctcgca ggcggtcggc
       61 cgcgtcgcgc gtgaaggccc ccggctcaac cgggggtggt cgcgggaaac gggtcgactg
      121 gaggtaggta gggggacgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggc ggcgaaggcg gcgtcctgga cctggcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01011398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011398
VERSION     KHUT01011398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c           98 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtcccg
       61 caagtcgggt gtgaaatccc aaggctcaac cttggaactg catccgatac tgctggactt
      121 gaggcaggta ggggaaggtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gccttctggg cctgtcctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011399
VERSION     KHUT01011399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           91 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgtta tccggattta ctgggcgtaa agcgcttgta ggtggtctcg
       61 taagttgggc gtgaaagctc cgggcttaac tcggagatgc cgtccaatac tgcgaggctg
      121 gaggatgtca gaggaaggtg gaattcctgg tgtagtggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gccttctggg acattcctga cactgagaag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011400
VERSION     KHUT01011400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           83 g           51 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcatgg
       61 taagtcaaat gtgaaatcct gcagcttaac tgtagaatag cattcgaaac taccaagctc
      121 gagggctgga gaggagatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg gatctctgga cagtctctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011401
VERSION     KHUT01011401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           99 g           43 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtaa ctgggcgtaa agggtccgta ggcggttggg
       61 gaggtctgat gtgacagcgt gcggctcaac tgcacgaggc cattggaaac ccctcaacta
      121 gcatcttcta gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg ggcagatgga cgctgaggga cgaaggcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011402
VERSION     KHUT01011402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          102 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 cgagtccggg gtgaaagccc ccagcttaac tggggaaggg cctcggatac cggcgggctc
      121 gagggaggta ggggcagcgg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg cgctgctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011403
VERSION     KHUT01011403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           43 a           64 c          113 g           33 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccgct
       61 taaggccggc gtgaaagccc ccggctcaac cggggagggt cgtcggcgac tgggcggctt
      121 gagggcggca ggggtcggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccgactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011404
VERSION     KHUT01011404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          104 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaacgt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggaaggc ggctgcgaac tggcgttctt
      121 gagggcgata gaggggggcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccccctggg tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011405             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011405
VERSION     KHUT01011405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           84 g           55 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggtctta
       61 ttagtctcgc gtcaaagctt cgggcttaac ctgaaaactg cgcgggaaac ggtaagacta
      121 gagggtgcga ggggtacgta gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaataccaaa agcgaaggca gcgtactggc gcatttcctg acactgaaac acgaaagcat
      241 gggtagcgaa tggg
//
LOCUS       KHUT01011406             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011406
VERSION     KHUT01011406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          101 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgt
       61 cgagtcaggc gtgaaagtcc cgggcttaac ctgggggcgg cgcttgagac tgacgggctt
      121 gaatgcggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggc ccgatcattg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011407             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011407
VERSION     KHUT01011407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           90 g           49 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggaatta ctgggtgtaa agcgtgcgta ggcgggtgtt
       61 caagtgagat gcgaaatcct gtggctcaac catatggact gtatctcata ctggatgcct
      121 ggaggacggc agaggcaagt ggaactggcg gtgtagcagt gatatgcgta gagatcgcca
      181 agaacaccaa tggcgaaggc agcttgctgg gccgtccctg acgctgaggc acgaaagcgt
      241 ggggagcgaa ctgg
//
LOCUS       KHUT01011408             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011408
VERSION     KHUT01011408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtttc
       61 caagtctgtg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tggaaatctt
      121 gaggagggta ggggcatctg gaactgccgg tggagcggtg aaatgcgttg atatcggcag
      181 gaactccggt ggcgaaagcg aggtgctgga ccctttctga cgctgagtgc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011409             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011409
VERSION     KHUT01011409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcgcgta ggcggcggcg
       61 taagttggag gtgaaatccc atcggctcaa ccgtggaatt gcccccaata ctgcgtcgct
      121 cgagggggat aggggcacgt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacgtgctgg gtcttctctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011410             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011410
VERSION     KHUT01011410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           75 g           59 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtagttgaa
       61 caagttactg gtggcaaatc ggggctcaac tccgtggaag tcagtaatac tattcaactc
      121 gaggactgga gaggttaata gaattctatg tgtagcggta aaatgctcaa atatatagag
      181 gaataccaat cggcgaaggc agttaactat tcagttcctg acattgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01011411             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011411
VERSION     KHUT01011411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           99 g           49 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgca ggcggctttc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tgggaggctt
      121 cgagtgcggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gagatcagga
      181 ggaacaccgg cggtgtagac ggctctctgg accgtgactg acgctgagac acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011412
VERSION     KHUT01011412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctctt gtgaaagctc ccggctcaac tgggagaggt caagggaaac tgccgggcta
      121 gagtgtggca gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccgtcactga cactgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011413
VERSION     KHUT01011413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           90 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccata
       61 caggtcagct gtgaaaactc aaggctcaac cctgagacgt cggttgaaac cgtatggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011414
VERSION     KHUT01011414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggttgga
       61 taagttagat gtgaaagccc cgggctcaac ctgggaattg catttaaaac tatccagcta
      121 gagtcttgta gaggggggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccggt ggcgaaggcg gccccctgga caaagactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011415
VERSION     KHUT01011415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           46 c           80 g           51 t
ORIGIN      
        1 tacagagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggtaaga
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tgtctttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttatacctga cgctaatgcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01011416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011416
VERSION     KHUT01011416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctcgt
       61 taagtcggat gtgaaaaccc ggggctcaac cccgggcctg catccgatac tggcgggctc
      121 gaggcagaca ggggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagt ggcgaaggcg gctctctggg tctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011417
VERSION     KHUT01011417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctcgt
       61 taagtcggat gtgaaatccc tcggctcaac cgaggaagtg cattcgatac tggcgagctt
      121 gaatatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011418
VERSION     KHUT01011418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcggag
       61 taagtcggat gtgaaatctc cgggctcaac ccggaaaatg cattggaaac tgctttgctc
      121 gagggttgga agggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011419
VERSION     KHUT01011419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           88 g           46 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggattta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtggggt gtgaaagccc tcggctcaac cgaggaattg cgctccaaac taccgtgctc
      121 gagggagata gaggttagcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gctaactggg tcttctctga cgctgatgca cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011420
VERSION     KHUT01011420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           86 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggttgga
       61 taagtctgat gtgaaatctt cgagcctaac tcggaaactg catcggaaac tattcggcta
      121 gagggttgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caactcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011421
VERSION     KHUT01011421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgt
       61 tgtgtctgtg gtgaaagccc cgggcttaac ctgggaattg ccgtggaaac tggcgagctc
      121 gagtctggca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaagactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011422
VERSION     KHUT01011422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           86 g           54 t
ORIGIN      
        1 tacgtaggga gcaagcgtta tccggattta ctgggtgtaa agggtgcgta ggcggctttg
       61 caagtttgat gtgaaaccca agggcttaac ctttggcatg catcgaaaac tgtagagcta
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt agcgaaggcg actttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011423
VERSION     KHUT01011423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           81 g           54 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggcatac
       61 taagtcaact gtttaattct ccggcctaac cggaggtcag cggtagaaac tggtaagcta
      121 gagtatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaagca gcttcttggt ccattactga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011424
VERSION     KHUT01011424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           43 c           95 g           57 t
ORIGIN      
        1 tacgtagggt gcgagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tacgtctgct gtgaaatcta ggggcttaac ttctagcgtg cagtgggtac gggcaggctt
      121 gagtgcggta ggggagattg gaatttctgg tgtagcggtg gaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gatctctggg ccgttactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01011425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011425
VERSION     KHUT01011425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           85 g           52 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggtggattgg
       61 taagtaagga gtgaaatccc agggcttaac cctggaactg ctctttagac tgcctgtctg
      121 gaattcggaa gaggttggtg gaactcccag tgtagaggtg aaattcgcag atattgggaa
      181 gaacaccggt ggcgaaggcg accaactggt ccgacattga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011426
VERSION     KHUT01011426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           89 g           46 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggcgatctt
      121 gagccaccta agggcaactg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaat ggtgaagaca ggttgctggg ggtgcgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011427
VERSION     KHUT01011427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 caagtcgggt gtgaaatacc tcagctcaac tgaggaattg cgcccgaaac tgctgtgcta
      121 gaggaagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactgga tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011428
VERSION     KHUT01011428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatta ttgggcgtaa agcgtacgca ggcggcttgg
       61 caagtcgagt gtgaaatccc ggggctcaac tccggaactg cattcgatac tgctaggctt
      121 gagtgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011429
VERSION     KHUT01011429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           95 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggcgata
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagcg catcgcaaac gatattgctg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttaatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011430
VERSION     KHUT01011430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           84 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtgact
       61 caagtcagct gtgaaatccc cgggcttaac ctgggaactg cggttgaaac taggtcacta
      121 gagtttggga gagggaagta gaattccatg tgtagcagtg aaatgcgtag atatatggag
      181 gaataccgat ggcgaaggca gcttcctggc ctagaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011431
VERSION     KHUT01011431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           84 g           50 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggctgttcgg
       61 caagttggaa gtgaaagccc agggctcaac cctggaattg ctttcaaaac tgccggactc
      121 gaatctcgga gaggatagcg gaatttccag tgtagtagtg aaatacgtag atattggaag
      181 gaacaccagt ggcgcaagcg gctatctgga cgagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011432
VERSION     KHUT01011432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggttttc
       61 taagtcaggt gtgaaatccc ccggcttaac cggggaagtg cgcctgaaac tgggaggctt
      121 gagtatcgga gaggaaaggg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggc ggcgaaggcg cctttctggt cgatcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011433
VERSION     KHUT01011433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttcg
       61 caagtcgagt gtgaaatccc cgggcttaac ccgggaactg cattcgagac tgcgttgcta
      121 gagtatggga gagggaggtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaagcg acctcctgga ccaatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011434
VERSION     KHUT01011434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           84 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgca ggcggactgg
       61 taagtcagtg gtgaaagccc tgggctcaac ccgggaactg ccattgatac tatcagtctt
      121 gaatatcgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaag cgaagattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011435
VERSION     KHUT01011435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtccgat gtgaaacctc ggagctcaac tccgagcctg cattggatac tgcctcgctt
      121 gagtccggga ggggagagcg gaattcctag tgtagcggtg aaatgcgcag atattaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011436
VERSION     KHUT01011436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           79 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggtcgta
       61 taagtcggat gtgaaatcct agggctcaac cctagaacag catctgaaac tgtatgacta
      121 gaggatagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatc ttattcctga cgctaaagcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01011437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011437
VERSION     KHUT01011437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           74 g           58 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggactta
       61 caagtcagtg gtgaaatctc cccgctcaac ggggaaactg ccattgaaac tatttgtctt
      121 gagtacattt gaggcgggcg gaatgtgtca tgtagaggtg aaattcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011438
VERSION     KHUT01011438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           97 g           51 t
ORIGIN      
        1 gacgtaggat gcgagcgttg ttcggaatta ttgggcgtaa agcgtgcgta ggcggcttgt
       61 taagtctggt gtgaaagcta ggggctcaac ccctgtgttg cactggaaac tggcaggctt
      121 gagtgcggta ggggaaggtg gaattccgag tgtagcggtg aaatgcgtag atattcggaa
      181 gaacaccggt ggcgaaggcg accttctggg ccgccactga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011439
VERSION     KHUT01011439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ttcggaatca ctgggcgtaa agcgggtgta ggtggctttg
       61 taagtcacat gtgaaagccc tgggctcaac ccaggaagtg catgtgatac tgcaaagctt
      121 gagtactgga gaggatggta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccatctggc caagtactga cactcagacc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011440
VERSION     KHUT01011440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcga
       61 aaagtcggcg gtgaaaactc gggactcaac tccgaggcgc ccgtcgatac tgtcgagctt
      121 gagtccggta ggggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gcactctggg ccggcactga cgctgaggag cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011441
VERSION     KHUT01011441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           91 g           53 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttga
       61 taagtgagat gtgaaatctc ggggcttaac tccgaaagtg catttcaaac tgtcaggcta
      121 gagtgcagta gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011442
VERSION     KHUT01011442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctatt
       61 caagtcagag gtgaaagccc gtggctcaac cacggaattg cctttgaaac tgtatagctg
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011443
VERSION     KHUT01011443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           85 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttag
       61 aaagtcagat gtgaaatccc cgggctcaac ctgggaactg catatgaaac taataagcta
      121 gagtgtggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccataactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011444
VERSION     KHUT01011444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 tacgtacgag gcaagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggcggatcgc
       61 taagtagggc gtgaaatctc ccggcctaac tgggaggggt cgccctaaac tggcagtctt
      121 gaggacagca gaggtaggtg gaattcctgg tgtagtggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcctactggg ctgtctctga cgctgaggcg cgaaagctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011445
VERSION     KHUT01011445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttac
       61 caagtcagac gtgaaagcct ggggctcaac cccagaagtg cgtttgaaac tggtaggcta
      121 gagtgcatta gagaatggcg gaattcccag tggagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatttggg atgcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011446
VERSION     KHUT01011446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgc
       61 taagttaggt gtaaaattcc tgggcttaac ctgggcttga gacttaaaac tggcgaactg
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc cagatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011447
VERSION     KHUT01011447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           43 a           63 c          113 g           34 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcgcgg
       61 taagcatggc gtgaaagccc ccggctcaac cggggagggt cgccgtggac tgccgtgctt
      121 gagggcggca ggggtcggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccgactggg ccggccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011448
VERSION     KHUT01011448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           96 g           37 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggtggctctc
       61 cacgtcgggc gtgaaagccc ggggctcaac cccggcagtg cacccgatac gagggagcta
      121 gagtgctgga gaggcgggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg acccgctggc cagcaactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011449
VERSION     KHUT01011449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggtggctcga
       61 caagtctggt gtgaaagccc acggctcaac cgtggaactg cattggaaac tgctgagctc
      121 gagtattgga gggggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggc catttactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011450
VERSION     KHUT01011450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           90 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggattg
       61 taagtcagat gtgaaattcc agagcttaac tctggcgctg catctgaaac tgcgattctt
      121 gagtgctgga gaggatagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctatctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011451
VERSION     KHUT01011451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatta ctgggcttaa agggcgcgta ggtgtcccga
       61 caagtcagat gtggaatctt ttcgcttaac gaaaaaacgg catctgaaac tatcgggatt
      121 gagtcagaca ggggatggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaaggccggt ggcgaaggcg gccatctggg tctgaactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011452
VERSION     KHUT01011452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           96 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgct
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaattg cattttagac tgagtgactg
      121 gagtgcagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg accacctgga ctgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011453
VERSION     KHUT01011453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           91 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agcgcgcgta ggcgggtaat
       61 taagatagat gtaaaatacc tgggctcaac ctaggtggga catttaggac tggttatctg
      121 gagtagagga gaggggagta gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaatacccgt ggcgaaggcg actctctggt ctcatactga cgctgaggtg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011454
VERSION     KHUT01011454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacggagggg gcaaacgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggttcga
       61 taagtctagt gtgaaatctc tcagcttacc tgagaaactg cgctggaaac tatcgagctc
      121 gaggatggaa gaggcaggtg gaatttctgg tgtagcggtg gaatgcgtag atatcagaaa
      181 gaacactcgt ggcgaaggcg gcctgctggt ccatttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011455
VERSION     KHUT01011455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           98 g           40 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccgc
       61 taagtctggt gtgaaatccc ccggctcacc cggggaagcg cgccggaaac tgacggactc
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011456
VERSION     KHUT01011456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           94 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggacctt
       61 taagtggatg gtgaaatgcc ggggctcaac cccggaactg ccttccaaac tggaggtctt
      121 gaggtatgga gagggatgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcatcctggc catattctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011457
VERSION     KHUT01011457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggattgc
       61 taagttggat gtgaaatccc tgggcttaac ctaggactgg catacaaaac tggcgatctg
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011458
VERSION     KHUT01011458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           74 g           59 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggaatta ttgggcgtaa agcgtctgca ggtggagaat
       61 taagtctttt gttaaatctt cgggcttaac ccgaaatcag caaaagaaac tatttttctt
      121 gagtatggta gaggtagagg gaatttccag tggagcggtg aaatgcgtag atattggaaa
      181 gaacaccaag agcgaaggca ctctactggg ccattactga cactcagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011459
VERSION     KHUT01011459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacgtatggg gctagcgtta tccggattta ttgggcgtaa agagcgcgta ggcggtttgt
       61 taagtcagat gtgaaaaccg ggggctcaac ccgcggcctg catctgaaac tggcagactt
      121 gagtctggta gaggaaagtg gaattcctag tgtagcggtg aaatgcgcag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctggg ccacgactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011460
VERSION     KHUT01011460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           97 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtgata
       61 tgtgaatggt gtgaaagccc cgggcttaac ctgggaagag catcgttgac tatatgactg
      121 gagtagatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011461
VERSION     KHUT01011461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           85 g           54 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgca tgtggctttg
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgctgga gaggtaagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccgga ggcgaaggcg gcttactggc cagacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011462
VERSION     KHUT01011462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           95 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgattgtg
       61 caagtctcgt gtgaaagccc acggcttaac cgtggaagtg cacgagagac tgcatgattt
      121 gagtacagga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011463
VERSION     KHUT01011463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           84 g           58 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agggtttgta ggctgaaaga
       61 taagtcaaat gtgaaatccc cgagctaaac ttgggacgtg catttgatac tgtttttctt
      121 gagtatcgta gagggtggtg gaattgtagg tgtaggagtg acatccgtag agatctgcag
      181 gaacatcaga ggcgaaggcg accacctggc cgattactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011464
VERSION     KHUT01011464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           74 g           64 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggacttt
       61 taagtcagtg gtgaaagcct gcagctcaac tgtagaattg ccattgaaac tgattgtctc
      121 gagtgtggtt gaggtcactg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca ggtgactaag ccattactga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011465
VERSION     KHUT01011465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcagccccg
       61 cgagtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgatac tgcggggctt
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011466
VERSION     KHUT01011466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           88 g           56 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agagtatgta ggcggccaga
       61 taagtcaggt gtgaaatccc cgggctcaac ccgggaagtg catttgatac tgtttggctt
      121 gagtatggaa gagggtagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctggt cctatactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011467
VERSION     KHUT01011467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagtcaggg gtgaaatcct ccggctcaac cggagaagtg cctttgatac tggcggcctc
      121 gagggaggta ggggtctgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcggactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011468
VERSION     KHUT01011468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagg
       61 taagtcagtg gtgaaatctc tgggctcaac ccagaaactg cctttgatac tatctgcctt
      121 gaatattgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactacg catatattga cgctgatgca cgaaagcgtg
      241 aggatcaaac agg
//
LOCUS       KHUT01011469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011469
VERSION     KHUT01011469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          104 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtggct
       61 taagcgtgtg gtgaaagctt cgggctcaac ccggagtcgg ccatgcgaac tggggcactg
      121 gagcatgtca gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg acattgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011470
VERSION     KHUT01011470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          102 g           42 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggtggcgcgt
       61 caagtctggt gtgaaagcct ccggctcaac cggaggaggc cactggagac tggcgtgctc
      121 gaggtgggta ggggtcggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gccgactggg cccaacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011471
VERSION     KHUT01011471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggcttg
       61 taagtgagat gtgaaatccc tgggctcaac ccaggaactg catttcaaac tgcaggtctc
      121 gagtgacgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgtccactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011472
VERSION     KHUT01011472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcagcg
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgcgttgctt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gccaactgga cgaaaactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011473
VERSION     KHUT01011473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          105 g           34 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccgcc
       61 cgcgtcggac gtgaaagccc ggggctcaac cccggaacgg cgtccgatac ggggcggctc
      121 gaggccggga gaggggggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gccccctggc ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011474
VERSION     KHUT01011474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           89 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggctgga
       61 aaagtcggag gtgaaatccc ggggctcaac cctggaactg ccctcgatac tttccggcta
      121 gagaactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011475
VERSION     KHUT01011475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           98 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgca ggcggttgga
       61 caagggtagt gtgaaatccc cgggcttaac ccgggaacag cattacagac tgtctggctg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctgtctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011476
VERSION     KHUT01011476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggcatgta ggtggtttta
       61 acagtttgat gtgaaagccc cgggcttaac ctgggaactg cgttgaatac tataagacta
      121 gagtcggtca gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gctacctggg accagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011477
VERSION     KHUT01011477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggttatt
       61 taagtcagtg gtgaaagctc attgcttaac aatgaaactg ccattgaaac tgagtaactt
      121 gagtatagat gaagtgggcg gaatacatca tgtagcggtg aaatgcatag agatgatgta
      181 gaacaccgat tgcgaaggca gctcactaat gtataactga cgctgaggca cgaaagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01011478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011478
VERSION     KHUT01011478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtcgtg
       61 cacgtttggg gtgacagctt ccggcttaac tggaagagga cctcgaagac ggcgcgactt
      121 gaggggttca gagggaccga gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaatacccaa ggcgaaggca tggtcctggg aaccatctga cgctcaggtg cgaaagcgag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011479
VERSION     KHUT01011479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggcggcttca
       61 taagtcagga gtgaaagccc tgggctcaac ccaggaagtg cttttgatac tgtgaagctt
      121 gagtgtggga gaggatagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccgga ggcgaaggcg gctatctggc ccaacactga cgctcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011480
VERSION     KHUT01011480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           83 g           54 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggttggt
       61 aaagtgggtg attaaagacc gatgcttaac atcgggactg ttgcccatac tcaccaacta
      121 gaggatgaca ggggtaagcg gaactttagg tgtaggagtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttactggg tttttcctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011481
VERSION     KHUT01011481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           93 g           42 t
ORIGIN      
        1 tacgggggga gcgaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcctcg
       61 taagtggaat gtgaaagccc ctggcccaac cagggaaatg cgtcccatac tgcgaggctt
      121 gagtgcagaa gaggatgggg gaattcccgg tgtagcggtg aaatgcattg atatcgggag
      181 gaacaccagt ggcgaaggcg cccatctggt ctgctactga cgctcatgcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011482
VERSION     KHUT01011482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           75 g           68 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatatt
       61 taagtcagag gtgaaatctc caggctcaac ctggaaactg cctttgatac tatttatctt
      121 gaattttgtt gaggttggca gaatatatca tgtagcggtg aaatgcatag atatgatata
      181 gaataccgat tgcgtaggca gctggctgaa caacgattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011483
VERSION     KHUT01011483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgtccgca ggtggtccac
       61 caagtttggg gtgacagttc ccggctcaac tgggaaagcg cttcgaaaac tggcggactt
      121 gagggcttga gaggaaagcg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgtaggca gctttctggc aagtacctga cactgatgga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011484
VERSION     KHUT01011484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtag
       61 aaagttgggt gtgaaatccc ccagctcaac tggggaacgg cgcccaaaac ttctgcgcta
      121 gaggaagata gaggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgtaagcg gcgtactggg tcttatctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011485
VERSION     KHUT01011485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtttat
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaatgg catcttaaac tgatggactg
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011486
VERSION     KHUT01011486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          105 g           42 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgca ggcggctgcg
       61 cacgttcgct gtgaaagctc ccggctcaac tgggagaggt cggcggatac ggcgcagctg
      121 gagctcggta gggggtcgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgaagctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01011487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011487
VERSION     KHUT01011487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           88 g           55 t
ORIGIN      
        1 tacgaggggt gcgagcgttg ctcggaatca ttgggcgtaa agcgagtgta ggtggtcata
       61 taagtcagtg gtgaaatccc tgggctcaac ccaggaagtg cctttgatac tgtatgactt
      121 gagtgtggga gagggtgcta gaattcctgg tgtagtggtg aaatacgtag agatcaggag
      181 gaacatcggt tgcgaaggcg ggtgcctggc ccaacactga cactcagact cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011488
VERSION     KHUT01011488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           81 g           57 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ttgggcgtaa agcatgcgta ggcggcttag
       61 taagttaaaa gtgaaagccc agagctcaac tttggaatgg cttttaatac tgctaagcta
      121 gagtgtcaga ggggatagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccaga ggcgaaagcg gctatctgga tgaatactga cgctgttgca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011489
VERSION     KHUT01011489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           83 g           58 t
ORIGIN      
        1 tacagaggga gctagcgttt ttcggaatta ttgggcgtaa agagcgtgta ggcggccgat
       61 taagttgaaa gtgaaatccc agagctcaac tctggaactg ctttcaaaac taattggcta
      121 gagttcggta ggagatgatg gaattcttgg tgtaggggtg aaattcttag atatcaggag
      181 gaacaccggt ggcgaaagcg atcatctggg ccgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011490
VERSION     KHUT01011490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           96 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtccg
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgcgggtctt
      121 gggccatcta ggggcgagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaat ggtgaaaaca gctcgctggg gatgtgccga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011491
VERSION     KHUT01011491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgcgta ggcggctcag
       61 taagtccgct ctgaaaactt ggggcttaac cccaagaggc gggcggatac tgctgggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011492
VERSION     KHUT01011492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           52 c          105 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggcagtt
       61 taagtcggcc gtgaaatctc caggcttaac ctggaggggt cgggcgagac tggattgctc
      121 gaggtgggca gagggcggtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctggg cctggcctga cgctgagacg cgaaagcgtg
      241 gggagcaatc cgg
//
LOCUS       KHUT01011493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011493
VERSION     KHUT01011493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           81 g           58 t
ORIGIN      
        1 gacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtatgta ggcggtagat
       61 taagtctggg gtaaaaggcg ttagcttaac taacgaagtg cccttgatac tgatgtacta
      121 gagacttaac gaggtaggcg gaatttatgg agtagcggtg aaatgcatag ataccataag
      181 gaaccccgat agcgaaggca gcttactggt taagatctga cgctgatgta cgaaggcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011494
VERSION     KHUT01011494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          100 g           41 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggttctg
       61 tgcgtccgcg ctgaaatctg tcggctcaac cggcaggcgc gtgcggatac gatgggactt
      121 gaggctctga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag atatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc agagacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011495
VERSION     KHUT01011495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           98 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgt
       61 tgcgtcaggt gtgaaagccc cgggcttaac ctgggatgtg cacttgatac gggcgagctg
      121 gaatccggaa gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctggt ccggtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011496
VERSION     KHUT01011496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           88 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcactt
       61 caagtctgct gtcaaagaca gtggcttaac tactgaaagg cagtggaaac tgaagcgcta
      121 gagtgtcgta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga cgacaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01011497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011497
VERSION     KHUT01011497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           94 g           53 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttaa
       61 caagtctgtt gtgaaatccc ggggcttaac tccggacgtg cagtggaaac tgttaggcta
      121 gagtgcagta gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011498
VERSION     KHUT01011498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          106 g           36 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggttcat
       61 gatgtgccgt gtgacagacg ggggctcaac ccccgcaggc catggtagac aggtggactg
      121 gaggcaggga gaggactgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcagtctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01011499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011499
VERSION     KHUT01011499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           81 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ctgggcgtaa agtgcacgta ggtggcttgg
       61 taagttagac gtgaaatccc tcgactcaat cgaggacatg catctaatac tgctaggcta
      121 gagtatagga ggggagaacg gaatttctgg tgtagaggtg aaattcgcag atatcagaag
      181 gaacaacagt ggcgaaagcg gttctctgga ctataactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011500
VERSION     KHUT01011500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgtcg
       61 taagttggat gtgaaatctc ggggctcaac cccgaaactg cattcaatac tgcgatgctc
      121 gagtactgga gaggagactg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaaggccagt ggcgaaggcg ggtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011501
VERSION     KHUT01011501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          104 g           38 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggagcga
       61 ccagtccggc ctgaaagccc ccggctcaac cggggagggt ggctggaaac tgtcgttctt
      121 gagggcgaga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgg ggcgaaggcg gccctctggc tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011502
VERSION     KHUT01011502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           84 g           47 t
ORIGIN      
        1 tacgaaggtc ccaagcgtta ttcggaatca ctgggcgtaa agggtgcgta ggtggtgagg
       61 aaagtcagat gtgaaagccc cgggctcaac ctgggaactg catccgatac tccctcacta
      121 gaggattgga gaggagtctg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactcgt ggcgaaagcg agactctgga caattcctga cactgatgca cgaaggccgg
      241 gggagcgaac agg
//
LOCUS       KHUT01011503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011503
VERSION     KHUT01011503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c          101 g           32 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgagca ggcggctcga
       61 tgcgtccgat gtgaaagcgc ccggctaaac cgggcgaggc cattggaaac ggtcaagctg
      121 gagggcagta gaggcaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctgctggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011504
VERSION     KHUT01011504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggactgg
       61 taagtcaggg gtgaaatctt cgagctcaac tcggaaactg cctttgatac tatcagtctt
      121 gaatattgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctacg catatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011505
VERSION     KHUT01011505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           38 c           82 g           59 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtttgta ggcggttata
       61 caagtcagtg gtgaaagcct ggggctcaac ctcagaattg ccattgaaac tgtatagcta
      121 gagaatgtta gaggaaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gctttctggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011506
VERSION     KHUT01011506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          103 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtccggg gtgaaatcca ccggctcaac cggtgaacgg cctcggatac tggcggcctg
      121 gaggcgggta ggggcgggcg gaactctggg tggagcggtg aaatgcgttg atatccagag
      181 gaactccggt ggcgaaggcg gcccgctgga cccgatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011507
VERSION     KHUT01011507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggttatt
       61 taagccagat gtgaaagccc cgggcttaac ctgggaatgg catttgggac tggatagcta
      121 gagtggggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccagcactga cactcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011508
VERSION     KHUT01011508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggtacgca ggcggcttac
       61 caagtcaggg gtgaaatccc ggggctcaac cccggaactg cctttgaaac tggtaggcta
      121 gagtatggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctcactggc ccataactga cgctcaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011509
VERSION     KHUT01011509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtccc
       61 caagtcaggg gtgaaatact ccggctcaac cggagcagtg cttctgatac tggggacctc
      121 gaggggtgca ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011510
VERSION     KHUT01011510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 tacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgtga
       61 caagtcggaa gtgaaatctc taggctcaac ttagagactg ctcccgaaac tgccatgcta
      121 gagtgcggga gaggccagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctggctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011511
VERSION     KHUT01011511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgc
       61 caagtcttgt gtgaaagccc cgggctcaac ccgggaagtg cgcaagatac tggtgagctg
      121 gagtgtcgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgataactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011512
VERSION     KHUT01011512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           54 c          104 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggtccgt
       61 ttagtctgct gtgaaagctc ctcgcttaac ggggagaggt cggtggatac tggcggactg
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011513
VERSION     KHUT01011513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          103 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggaccc
       61 caagtccgct gtgaaagccc gaggctcaac ctcggagggt cggtggatac tggggttctt
      121 gaagatgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag agatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011514
VERSION     KHUT01011514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           89 g           46 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcttcg
       61 aaagtcggat gtgaaatccc tcggctcaac cgaggacgtg catccgaaac tgtgaagctt
      121 gagtactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagttactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011515
VERSION     KHUT01011515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctgtg
       61 caagtcaggg gtgaaatccc tcggctcaac cgaggaactg cctctgaaac tgcacggctt
      121 gagaccggga gagggtggtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg accacctgga ccggatctga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011516
VERSION     KHUT01011516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           84 g           47 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtttgta ggcgatcctc
       61 cacgtttggt atgaaatgtc ggagcccaac tccggcactg tgccgaaaac gagaggaatt
      121 gagcaactca gaggcatctg gaatgtcatg tgtaggggta aaatccgtag atacatgatg
      181 gaacgccaaa agcgaaagca ggatgctggg agttcgctga cgctgagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011517
VERSION     KHUT01011517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           88 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtggtg
       61 taagtcaggt gtgaaatctc ggagcttaac tccgaaactg cacttgatac tgctctactt
      121 gaggactgga gaggaaaccg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg ggtttctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011518
VERSION     KHUT01011518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           93 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggcgcgc
       61 taagtcaggg gtgaaatccc gcggctcaac tgcggaactg cccttgatac tggcgtgctc
      121 ggatcggcgc gaggcaggcg gaacgtggca tgtagcggtg aaatgcttag atatgccaca
      181 gaacaccgat tgcgtaggca gcctgccaga gccgcatcga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011519
VERSION     KHUT01011519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 tacgtatggt gcgaacgttg ttcggaatca ctgggcttaa agcgggcgta ggcggttcgg
       61 taagtccgtt gtgaaagccc tcggctcaac cgaggaactg caacggatac tgccgggcta
      121 gagtgcagga ggggagagtg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggc ctgttactga cgctgaggcc cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011520
VERSION     KHUT01011520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggctcgt
       61 taagcctgat gtgaaatccc tgggctcaac ctgggaatgg catcggggac tggcgggcta
      121 gagtttggga gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccacctggc ctaaaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011521
VERSION     KHUT01011521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           89 g           54 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcctac
       61 caggtctgat gtgaaatctc acggctcaac cgtgaaattg cgttggaaac ctgtaggctt
      121 gagtgtgcta ggggtatgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acatactggg gcataactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011522
VERSION     KHUT01011522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctta
       61 taagtcatat gtgaaagccc acggctcaac cgtggaagtg catgtgaaac tgtgaggctt
      121 gagtatggga gaggaaagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg actttctgga ccaatactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011523
VERSION     KHUT01011523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcgggaagg
       61 taagtggtgt atgaaatcgc tcggctcaac cgggcgcggt tatgccagac tgccaatcta
      121 gagagcgaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgtcctggc tcgcatctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011524
VERSION     KHUT01011524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgtgta ggcgggagct
       61 taagtctgct gttaaatctc atagctcaac tatgaaatgc cggcggatac tgggcttctt
      121 gaattcggta gaggtaactg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacgccaaa ggcgaaggca ggttactggg ccgcaattga cgctgagaca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011525
VERSION     KHUT01011525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           75 g           65 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtttta
       61 taagtcagtg gtgaaagact gtcgctcaac gatagcattg ccattgatac tgtaggactt
      121 gagtatattt gaggtgggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccaat tgcgaaggca gctcactaaa ctataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011526
VERSION     KHUT01011526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ctgggtgtaa agggtgcgta ggcggctctg
       61 caagtcaggc gtgaaatata tgggcttaac ccatagactg cgtttgaaac tgtggagctt
      121 gagtgaagta gaggtaggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcctactggg ctttaactga cgctgaggca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011527
VERSION     KHUT01011527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           85 g           47 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtgtcctgc
       61 cacgtctgat gtaaaagcac gaagcccaac ttcgtgtacg tatcggaaac gagcgggatt
      121 gagtcaatca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgccaaa agcgaaggca ggatgctggg gttgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011528
VERSION     KHUT01011528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctccg
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgcagggcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacatcggc ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011529
VERSION     KHUT01011529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggttttc
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggaaggctt
      121 gagtatggga gaggcaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagaca gcttgctgga ccataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011530
VERSION     KHUT01011530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           49 c           99 g           51 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggtggtttgg
       61 caagttgggt gtgaaatccc ctggctcaac tggggggggt catccgatac tgccagactt
      121 gaggcaggca gaggaaggta gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctggg cctgtcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011531
VERSION     KHUT01011531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           88 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaatttc ggagctcaac tccgaaactg cattggatac tattcggctc
      121 gaggttggga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga ccaatcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011532
VERSION     KHUT01011532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttga
       61 taagtcagat gtgaaagccc acggcttaac cgtggaagtg catttgatac tgtcagactt
      121 gagtaccaga ggggaaagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg actttctggc tggatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011533
VERSION     KHUT01011533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c          101 g           41 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgcgta ggcggggatg
       61 taagtgggtg gtgaaagcgc ccggctaaac cgggcgagga ccattcagac tgcatttcta
      121 gaggcggata gaggagcgtg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcagacg acgctctggg tccgaactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011534
VERSION     KHUT01011534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           97 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctatc
       61 taagtccgat gtgaaagccc ggggctcaac tccggaaggg cattggaaac tgggtagctt
      121 gagttcggga gaggaaggca gaattcccgg tgtagggttg aaatccgtag atatcgggag
      181 gaaaaccggt ggcgaaggcg gccttctgga acgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011535
VERSION     KHUT01011535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           87 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggtgtaa agggtgcgta ggcggtaatg
       61 taagtcaggt gtgaaagccc gaagctcaac ttcgggattg cacttgaaac tacataacta
      121 gagtacagga gaggtgagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actcactgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011536
VERSION     KHUT01011536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           84 g           60 t
ORIGIN      
        1 tacgagtggt gctagcgtta ttcggattta ttgggcgtaa agggtgcgta gacggtatga
       61 caagtctgtt gttaaattcc tcggctcaac tgaggatctg cgatggaaac tgtcgtgcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011537
VERSION     KHUT01011537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacgtaggga cctagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggcttag
       61 caagtccggt gtgaaagctc ggggctcaac cccgagtctg cattggaaac tgctgagctt
      121 gagtccggaa gaggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccggt ggcgaaggcg ggtctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011538
VERSION     KHUT01011538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           99 g           37 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtggtg
       61 ccagtccgtt gtgaaagccc cgggctcaac ctgggaacgg caagggcgac ggcacgacta
      121 gagtggagca gaggaccgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggac
      181 gaacaccgat ggcgaaggca gcggtctggg gtcacactga cgctgaggtg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011539
VERSION     KHUT01011539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           97 g           42 t
ORIGIN      
        1 aacgtaggag acaagcgtta tccggagtta ctgggcgtaa agggcgtgta ggcggcggaa
       61 taagtctgtt gtgaaagctc ctggcttaac cgggagaggc caatggagac tgtttcgctc
      121 gaggccggta gaggtgggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcccactagg ccggacctga cgctgaaacg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011540
VERSION     KHUT01011540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           79 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggatcga
       61 taagtcagtg gtgaaagccc cgagctcaac ttgggaattg ccattgatac tgttgatctt
      121 gagtgcggtt gaggtagacg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gtttaccaag ccgatactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011541
VERSION     KHUT01011541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggtgtaa agggcgtgta ggcggttgag
       61 taagtggaat gtgaaatccc taggcttacc ctaggacctg catcccatac tgctcgactt
      121 gagtacggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccgatactga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011542
VERSION     KHUT01011542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           85 g           47 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggcgtga
       61 aaagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tgttgcacta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01011543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011543
VERSION     KHUT01011543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtatgta ggtggcctcg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcgaggctc
      121 gagtgcggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgttactga cactgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011544
VERSION     KHUT01011544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagggggg gcgagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggctgac
       61 tcagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tagtcagctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgaggaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011545
VERSION     KHUT01011545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacagaggtt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcgggatag
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tactgttctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011546
VERSION     KHUT01011546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           88 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa aggatgtgta ggcggatatt
       61 taagtgagat gtgaaatccc cgagctcaac ttgggggctg catttcaaac tgggtatcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacatcagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011547
VERSION     KHUT01011547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagac gtgaaaaccc tgggcttaac ccaggacctg cgtttgatac tggagggctt
      121 gaggatcgga gaggacagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgtctgga cgacacctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011548
VERSION     KHUT01011548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 tacagaggcc ccgagcgtta atcggagtta ttgggcttaa agggtgcgta ggcggtcccg
       61 caagtgtctt gtgaaatccc acggctcaac cgtggaatgg ctcggcaaac tgcgggactc
      121 gaggttggta gaggtcgctg gaacgctagg tgtagcggtg aaatgcgtag atatctagcg
      181 gaacgccgaa ggtgaagaca ggcgactggg ccaattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011549
VERSION     KHUT01011549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 gacaggggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggcttct
       61 taagtctgct gtcaaatccc agggcttaac cctggccagg cggtggagac tactaagctt
      121 gagtacggta ggggcagagg gaatttccgg tggagcggtg aaatgcgtag agatcggaaa
      181 gaacaccaag ggcgaaggca ctctgctggg ccgacactga cactgagaga cgagagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01011550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011550
VERSION     KHUT01011550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           96 g           40 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggtcgt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaaccg ctgggcatac tggcgatctt
      121 gggccaccca ggggcaaccg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgac ggtgaaaaca ggttgctggg ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011551
VERSION     KHUT01011551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           54 c           79 g           50 t
ORIGIN      
        1 tacagagacc tcgagcatta tccggattta ttgggcgtaa agggtccgca ggtggccgtt
       61 cacgtcgatg gttaaatttc agggctcaac cctgaaaccg ccaccgatac ggttcggctc
      121 gagcatggga gaggtacgcg gaattaccgg tgtagtagta aaatgcgtta atatcggtaa
      181 gaacaccaaa tgcgaaggca gcgtactgga acattgctga cactcaggga cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01011552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011552
VERSION     KHUT01011552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           80 g           60 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacccg
       61 ttagtcagtg gtgaaagttt gcagctcaac tgtaaaattg ccattgaaac tgcgggtctt
      121 gagtgtaaat gaggtaggcg gaatgtgttg tgtagcggtg aaatgcttag atataacaca
      181 gaacaccaat tgcgaaggca gcttactggg atacaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011553
VERSION     KHUT01011553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgca ggcggacgtt
       61 taagtctgag gtgaaagctc ggagctcaac tccgaaatgg ccttggaaac tgagtgtctt
      121 gagggatgga gaggacagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctgtctgga catcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011554
VERSION     KHUT01011554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           91 g           44 t
ORIGIN      
        1 tacgtaggga gcgagcgttc tccggattta ctgggcgtaa agcgcatgca ggcggttgag
       61 caagtctcga ctgacagttt ccggcttaac cgggaaaggt gtcgggaaac tgctgaactt
      121 gagtcaccaa gaggcagagg gaattccgtg tggagcagtg aaatgcgtag agatgcggaa
      181 gaacaccaga ggcgaaggcg ctctgctggg ggtgaactga cgctaagatg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011555
VERSION     KHUT01011555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           78 g           57 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agggcaggta ggcggatatg
       61 ttagtcggat gttaaatctc agggctcaac cctgaacagg cattcgaaac ggcatgtcta
      121 gaggatgtga gagatcagtg gaatttatgg tgtagtagtg aaatgcgttg atatcataaa
      181 gaacacccaa ggcgaaggca actgattggc acatatctga cgctgagctg cgaaagcgtg
      241 gggagcgaat agg
//
LOCUS       KHUT01011556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011556
VERSION     KHUT01011556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggccagg
       61 taagttggac gtgaaaacgc cgggcttaac tcggcgaggc cgttcaatac tgccaggcta
      121 gagggcgtca gagggaagcg gaatacctgg tgtagcagtg aaatgcgtag atatcaggtg
      181 gaacatccgt ggcgaaggcg gcttcctggg gcgcacctga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011557
VERSION     KHUT01011557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           53 c           83 g           49 t
ORIGIN      
        1 gacgaaccat ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcccgg
       61 taagttgggt gtgaaatacc tcagcctaac tgaggaattg cgcccaatac tgtcgggctc
      121 gaggaagaca taggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt agcgaaagcg gctcactggg tcttatctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011558
VERSION     KHUT01011558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           97 g           46 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca gacggggcat
       61 caagtggtgt atgaaatcgc ccggctcaac cgggcggggt tatgccagac tggtgtgcta
      121 gagacagaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacccctga ggcgaaggcg gcgtcctggc tctggtctga cgttcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011559
VERSION     KHUT01011559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           85 g           47 t
ORIGIN      
        1 tacgtaggga gcaagcgtta tccggaatca ctgggcgtaa agagctcgta ggcggttaga
       61 taagtcgcat gttaaatacc caggctcaac caggggaatg cgtgcgatac tgtttagcta
      121 gaggacggga gaggtaagtg gaattcctgg tgtagcggtg aaatgcatag atatcaggaa
      181 gaacaccagt ggcgaaggcg gcttactaga ccgttcctaa cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011560
VERSION     KHUT01011560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcgacc
       61 taagtctgat gtgaaagccc ggggcttaac tccggaagcg cattggatac tgagtagcta
      121 gagtactgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011561
VERSION     KHUT01011561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           83 g           57 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggaatta ctgggcgtaa agggagagta ggcggaacgg
       61 taagttagaa gtgaaatgta cgggcttaac ccgtaatctg cttttaatac tgccattctt
      121 gagtatggga gaggatgata gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaataccagt tgcgaaggcg gtcatctggc ccaatactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011562
VERSION     KHUT01011562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c          100 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgcgtgta ggtggctggt
       61 tacgtctgct gtgaaatgtc ccggcttaac cgggactagg cggtagatac ggatcggcta
      121 gagggtgaca gaggagcgta gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccagt ggcgcaagcg gcgctctggg tcgagcctga cactgagacg cgaaggcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011563
VERSION     KHUT01011563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           94 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggtatgt
       61 taagtcaggt gtgaaatccc acggctcaac tgtggtgttg cacttgaaac tggcatgcta
      121 gagtgcagga gagggaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011564
VERSION     KHUT01011564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           79 g           59 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcagtg gtgaaagcct ggagcttaac tccagaactg ccattgatac tgatgggctt
      121 gagtacagtt gaggttggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctagctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011565
VERSION     KHUT01011565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaatag cttctgatac tgatcgtctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011566
VERSION     KHUT01011566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           84 g           55 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttt
       61 taagtacctt gtgaaatccc atggcttaac catggaactg ctgggtatac tggatatctt
      121 gagccaacta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg gttgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011567
VERSION     KHUT01011567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcaa
       61 taagtttgct gtaaaagtcg ggggctcaac ccccgaaagc cggtgaatac tgttggacta
      121 gagtgcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctat tgcgaaggca gctcgctggt acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011568
VERSION     KHUT01011568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggtgacg
       61 taagtcagat gtgaaagccc tgggcttaac ctgggaaagg catttgatac tgtgtcgctg
      121 gagtgcgtga gaggaaagcg gaattccagg tgtaggggtg aaatccgtag atatctggag
      181 gaataccgac ggcgaaggca gctttctggc acgtaactga cactgagatt cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011569
VERSION     KHUT01011569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          101 g           36 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtccgtg gtgaaatcct ccggcccaac cggagaacgg cctcggatac tggcggcctg
      121 gagggaggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga cctctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011570
VERSION     KHUT01011570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttgtt
       61 taagttagtt gtgaaatccc tgggcttaac ctaggaactg caactaagac tggatagctg
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011571
VERSION     KHUT01011571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggatcag
       61 taagtgtcgt gtgaaagccc ccggctcaac cggggaaccg catggcaaac tgctgatctt
      121 gaggaagcta gaggccggta gaacgattgg tggagcggtg gaatgcgtag atatcaatcg
      181 gaatgcctaa agtgaagaca gccggctggg gctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011572
VERSION     KHUT01011572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          101 g           42 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggttgga
       61 caagtggcgt gtgaaagcgc ccggctaaac tgggcgaggc caggtcagac tgttcgactc
      121 gagtccgata gaggggcgta gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaataccagt ggcgcaagcg gcgccctggg tcgggactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011573
VERSION     KHUT01011573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c          103 g           34 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatctg
       61 caagtcggag gtgaaatccc ggggctcaac tccggggctg cccccgaaac tgcaagtcta
      121 gggaccggta gaggccggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccggctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011574
VERSION     KHUT01011574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           92 g           52 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcttac
       61 caggtccggt gtgaaatctc tcggctcaac cgaggaattg cgctggaaac cggtaggctt
      121 gagtgagcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg gcttaactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011575
VERSION     KHUT01011575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggattgg
       61 caggtcagtg gtgaaatccc tcggctcaac cggggaactg cctctgaaac cgccagtctc
      121 gagggagaca ggggcatgtg gaacttccag tggagcggtg aaatgcgtag agattggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011576
VERSION     KHUT01011576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           85 g           56 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcctgt
       61 taagtcagag gtgaaaggtt gcggcttaac cgcagacatg cctttgatac tggcgggctt
      121 gaataaagtt gaggtaggcg gaatgtggca agtagcggtg aaatgcatag agatgccaca
      181 gaacaccgat tgcgaaggca gcttactaag ctttgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011577
VERSION     KHUT01011577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcggcgctg
       61 caagtgcctt gtgaaatccc atggcttaac catggaatgg ctcggtagac tgcagtgctt
      121 gaggtcagta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ctgaatctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011578
VERSION     KHUT01011578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           91 g           42 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgcgta ggcggaccgg
       61 aaagtcggtt gtgaaatccc tgggctcaac ctaggaactg catccgaaac tcccggtctc
      121 gagtgacgga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgtccactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011579
VERSION     KHUT01011579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           44 c           88 g           58 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggctag
       61 taagtcagtg gtgaaatccc gcagctcaac tgtggatgtg ccattgatac tgctggtctt
      121 gagtaccgtt ggggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat agcgtaggca gcttactgaa cggagactga cgctgaggca cgaaagcatg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011580
VERSION     KHUT01011580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          102 g           41 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggcctgc
       61 taagtcggct gtgaaagccc tcggcttaac cgaggaatgg cggccgagac tggcaggctt
      121 ggatccggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccggtatcga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011581
VERSION     KHUT01011581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           37 c           74 g           57 t
ORIGIN      
        1 tacggggggg actagtgtta ttcggaatga ctgggcgtaa agggtgtgta ggcggtcttg
       61 taagttgaaa gtgaaatctc aaagcttaac tttgaaagtg ctttcaaaac tgctcgacta
      121 gagtgtgaga gaggatagtg gaatttctag tgtagaaata aaatccatag agattagaag
      181 gaacaccgaa agcgaaggca actatctggc tcaaaactga cgctgataca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011582
VERSION     KHUT01011582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           75 g           59 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggcggattgg
       61 taagtcagtg gtgaaataca acagcttaac tgttgccctg ccattgatac tgtcagtcta
      121 gagtatagtt ggagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat cgcgaaggca gctcactaaa ctataactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011583
VERSION     KHUT01011583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           92 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttat
       61 taagttatct gtgaaagccc tgggctcaac ctgggaaggt cagataagac tggtagactt
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011584
VERSION     KHUT01011584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           38 c           71 g           66 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggtggacaat
       61 taagtcagag gtgaaatcct gcagctcaac tgtagaattg cctttgatac tggttgtctt
      121 gaatcaatgt gaagtggtta gaatatgtag tgtagcggtg aaatgcatag atattacata
      181 gaataccaat tgcgaaggca gatcactaac attgtattga cactgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011585
VERSION     KHUT01011585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           84 g           62 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggtgggcttt
       61 taagtcagtg gtgaaatgct gccgcttaac ggtagaattg ccattgatac tgagagtctt
      121 gagtatggtt gaggtaggcg gaatgtgttg tgtagcggtg aaatgcatag atatgacaca
      181 gaacgccgat tgcgaaggca gcttactaag ccattactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011586
VERSION     KHUT01011586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           83 g           56 t
ORIGIN      
        1 tacgtagacc tcgagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtctttt
       61 taagtctctg atgaaatctc cgggctcaac tcggaaactg ttagagaaac tggaaggata
      121 gagtatggca gaggcagatg gaatggttgg tgtagcggta aaatgcgttg atatcaacca
      181 gaacaccagt ggcgaaggcg atctgctggg ccattactga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011587
VERSION     KHUT01011587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           99 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcatgta ggcggtcatg
       61 caagtggcgt gtgaaagcgc ccggctaaac tgggcgagga cacgtcagac tgcatggcta
      121 gagggtgtta gaggggcgca gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaataccagt ggtgaagacg gcgccctggg acattactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011588
VERSION     KHUT01011588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccgat
       61 caagtcgggt gtgaaatccc tcggctcaac cgaggaactg cacccgatac tgcttggcta
      121 gagtccggga ggggatggcg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccggtactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01011589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011589
VERSION     KHUT01011589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           87 g           56 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggctgtcaat
       61 taagtcgggt gtgaaagccc tcggctcaac cgaggaagtg catccgaaac taattggcta
      121 gaagatggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctggg ccattcttga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011590
VERSION     KHUT01011590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcgggttta
       61 caagtctggc tttaaagacc gaggctcaac ctcgggaagg ggctggagac tgtgagtcta
      121 gacttctgga gaggcaactg gaattcttgg tgtagcggtg gaatgcgtag ataccaagag
      181 gaacaccaat ggcgaaggca ggttgctgga cagaaagtga cgctgaggcg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011591
VERSION     KHUT01011591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           99 g           43 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggggaa
       61 caagtcggtc gtgaaatcct ccggctcaac cggagagcgt cgatcgaaac tgttctcctt
      121 gaggattgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggg caattcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011592
VERSION     KHUT01011592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           76 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggataat
       61 taagtcaggg gtgaaagcct gcagctcaac tgtagaactg cccttgaaac tgattatctt
      121 gaatgtggtt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaggca gcttactaag ccattattga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011593
VERSION     KHUT01011593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           82 g           54 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggaatta ttgggcgtaa agagcgtgta ggtgttttta
       61 caagtcggtc gttaaatctc agagctcaac tctgagaccg cgagcgatac tgtaagaatt
      121 gaggtttgca ggggctagca gaatttctgg agtaggggtg aaatccgttg ataccagaag
      181 gaataccaat ggcgaaggca gctagctggg caatacctga cactgagacg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011594
VERSION     KHUT01011594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggtggcgtgg
       61 taagtcagat gtgaaagccc agggctcaac cctggaattg catccgatac tgctgtgcta
      121 gagtactgga ggggtgacta gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaataccaaa ggcgtaggca ggtcactgga cagttactga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011595
VERSION     KHUT01011595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttt
       61 caagtcatct gtagaatccc cgggctcaac ctgggaatgt cagctgatac tgtgagactg
      121 gagtacggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctgcctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011596
VERSION     KHUT01011596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagacaatg gttaaatacc cgggctcaac ccgggacatg ccactgtgac tgccagacta
      121 gacgatagca ggggcaagca gaattctcgg tgtaggggtg aaatccgttg agatcgagag
      181 gaataccaat ggcgaaggca gcttgctagg ctatatgtga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011597
VERSION     KHUT01011597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggtttgg
       61 aaagtctttt gtgaaagctc ccggcttaac tgggagaggt caaaggatac taccaggcta
      121 gagtgtagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtctctggg ctattactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011598
VERSION     KHUT01011598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           54 t
ORIGIN      
        1 tacagaggcc ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggatgg
       61 taagtacttt gtgaaatccc acggcttaac tgtggaattg cttagtatac tgccgttctt
      121 gagtcattta ggggcagccg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaat ggtgcagaca ggctgctggg aatgaactga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011599
VERSION     KHUT01011599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtccgt
       61 taagtgagaa gtgaaagctc cgggctcaac tcggaaacgg cttttcatac tggcggactc
      121 gagggtggca gaggctcgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccat ggcgaaggca gcgagctggg ccacatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011600
VERSION     KHUT01011600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           94 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggctgcg
       61 caagtcagat gtgaaagccc agggctcaac cttggaagtg catctgaaac tgcgcaacta
      121 gagtctcgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccggt ggcgaaggcg accgcctgga cgaagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011601
VERSION     KHUT01011601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 tacgaaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcggtcttg
       61 caagtcggat gtgaaatccc cgggctcaac ccgggaactg cacccgaaac tgcttgactt
      121 gaatgcttga gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcggt ggcgaaggcg accaactggt gatgcattga cgctgaggtg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01011602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011602
VERSION     KHUT01011602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          105 g           35 t
ORIGIN      
        1 gacagagggg gccagcgttg tccggaatga ctgggcgtac agcgcgcgca ggcggctggc
       61 tacgtgccgt gtgacagccc taggctcaac ctagggtcgc catggtagac gagtcggctg
      121 gagtgggtga gagggatgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagt ggcgcaagcg gcatcctggc accaaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011603
VERSION     KHUT01011603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           93 g           52 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggctgat
       61 taagtctgac gtgaaatctc ccggcttaac cgggagcggt cgttggaaac taatcggctc
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaacatcagt ggcgaaagcg gcaccctggc atttgcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011604
VERSION     KHUT01011604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           85 g           53 t
ORIGIN      
        1 gacgaaccac acgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggctgtgcag
       61 aaagttgggt gtgaaatccc tcagcttaac tgaggaactg cgctcaaaac tactgtgctt
      121 gagggagaca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011605
VERSION     KHUT01011605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctagg
       61 taagtggggt gttaaatccc ccggctcaac cggggaactg cacttcatac tgccaagctc
      121 gagtacagca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtctctggg ctgacactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011606
VERSION     KHUT01011606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtgggg
       61 taagtctggt gtgaaagctc ggagctcaac tccgaaatgt catcggaaac tacttcacta
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caaatcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011607
VERSION     KHUT01011607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           89 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggttacg
       61 tatgtccgag gtgaaagccc taagctcaac ttaggaagtg ccttggaaac ggcgtaacta
      121 gagtgctaga gaggttcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga tagcaactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011608
VERSION     KHUT01011608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcgaaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttagat gtgaaagtcc tgggcttaac ctggggattg cgtctgatac tgttaagcta
      121 gagttctgta gagggaggtg gaatttccgg agtagcggtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaagcg gcctcctgga caggaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011609
VERSION     KHUT01011609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           46 c           78 g           53 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agcgtgcgca ggcggtcatg
       61 aaagttagaa gtgaaatccc agagcttaac tttggaattg cttttaaaac tccatgacta
      121 gagtactgga gaggttagcg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccaat ggcgtaggca gctaactgga cagtaactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011610
VERSION     KHUT01011610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           96 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcgggttga
       61 taagagaatg gtgaaatccc tgggcttaac ctaggaatgg ccattgagac tgttgatctg
      121 gagtgcagta gagggtagtg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcggt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011611
VERSION     KHUT01011611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggttcga
       61 taagtctgct gtaaaagtcg agggctcaac cctcgaaagc cggtggatac tgtcgaacta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011612
VERSION     KHUT01011612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           82 g           54 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggactgc
       61 caagtcagat gtgaaatacc aaagctcaac tttggtgctg catttgaaac tggcagactt
      121 gagggttgac ggagaaaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg attttctagt taatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01011613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011613
VERSION     KHUT01011613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           48 c           83 g           47 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggagtga
       61 aaagtcagat gtgaaatccc gaggctcaac ctcggaacag catttgaaac tctcacccta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgagagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01011614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011614
VERSION     KHUT01011614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttac
       61 taagtcagat gtgaaatccc tgggctcaac cgaggaagtg catttgaaac tagtaggctg
      121 gagagtggta gagggaggtg gaattccgtg tgtagaggtg aaattcgtag atacacggag
      181 gaataccggt ggcgaaggcg gcctcctgga ccatttctga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011615
VERSION     KHUT01011615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcgag
       61 taagtctgat gtgaaatctc cgagcccaac tcggaaactg catcggatac tatccggctt
      121 gaggaatgaa ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011616
VERSION     KHUT01011616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           51 t
ORIGIN      
        1 tacgaggggt ccaagcgttg tccggaatca ctgggcgtaa agcgaatgta ggcggctttt
       61 caagtcactt gtgaaagccc ggggcctaac cccggaagtg caagtgatac tgttaagctt
      121 gagtatcaga gaggacactg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccggc ggcgaaggcg ggtgtctggc tgaatactga cgctgagatt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011617
VERSION     KHUT01011617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           81 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggctgcgtga
       61 aaagtcagat gtgaaatacg aaagctcaac tttcgaaatg catccgatac taccatgcta
      121 gagaactgaa ggggagcttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacacccgt ggcgaaggcg aagctctggt cagtatctga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011618
VERSION     KHUT01011618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggctgga
       61 caagtctggc gtgaaatcct ctggcttaac caggggcggt cgctggagac tgttcagctt
      121 gaggcgatga gaggggtgca gaattccagg tgtagtggtg gaatgcgtag atatctggag
      181 gaataccagt ggcgaaagcg gcaccctggc gtcgtactga cgcacatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011619
VERSION     KHUT01011619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           82 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggatggt
       61 taagtaagaa gtgaaaggtc ggggctcaac cccaacactg cttcttatac tggccgtctt
      121 gagttccgga gaggaaagcg gaactgcacg tgtagcggtg aaatgcgttg atatgtgcag
      181 gaacaccaat ggcgaaagca gctttctgga cgagaactga cgctcatgtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01011620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011620
VERSION     KHUT01011620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggggcgca ggcggggctg
       61 taagtcagtg gtgaaatacc tgagcttaac tcgggaactg ccattgatac tatagccctt
      121 gaatgacgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca gcaggctaaa cgtaaattga cgctgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011621
VERSION     KHUT01011621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           45 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggctgttcac
       61 caagtggtgt atgaaagcgc tcggctcaac cgggcaaggg tatgccagac tggtggacta
      121 gagactgaca gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gccacctggg tcagttctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011622
VERSION     KHUT01011622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           43 c           79 g           51 t
ORIGIN      
        1 gacgtaggat gcgaacgtta tccggaatta ctgggcgtaa agagttcgta ggcgaattgt
       61 taagtagatt gttaaagacc tgagcttaac tcagggaggg caatctaaac tgacagacta
      121 gagtacggca gaggcaagtg gaattctgtg tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gcttgctggg ccgttactga cgctcaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011623
VERSION     KHUT01011623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggggtgg
       61 taagtcagag gtgaaatctc cgagcttaac tcggaaactg cctttgatac tatcattctt
      121 gaatcatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactacg ggtgcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011624
VERSION     KHUT01011624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccgaa
       61 ttagtctgac gtgaaatccc ccggcccaac cggggaactg cgtttgaaac tgttcggctc
      121 gagtccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01011625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011625
VERSION     KHUT01011625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           93 g           43 t
ORIGIN      
        1 tacagggggt gcaagcgttg ctcggaatta ctgggcgtaa agcgtgcgca ggcggctcat
       61 caagtcgggg gtgaaagccc ggggctcaac cccggaattg ccttcgaaac tggtgagctt
      121 gaatgcggga gagggtcgcg gaattcttgg tgtagaggtg aaattcgtag agatcaggaa
      181 gaacaccagt ggcgaaagcg gcgacctggc ccgacattga cgctcatgca cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011626
VERSION     KHUT01011626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           88 g           55 t
ORIGIN      
        1 gacggggggt ccaagcgttg ctcggaatca ttgggcgtaa agggggcgta ggtggctttg
       61 taagtcagaa gtgaaagccc tgggctcaac ccgggaagtg cttttgatac tgcgaagctt
      121 gaatgtggta gaggatagta gaattcctag tgtagtggtg aaatacgtag atattaggag
      181 gaatacctgt ggcgaaggcg gctatctgga ccaacattga cactgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011627
VERSION     KHUT01011627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           83 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccggg
       61 taagtttgat gtgaaatctc cgagctcaac tcggaaattg cattgaatac tatccggcta
      121 gaggattgga ggggaaactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtttctgga caattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011628
VERSION     KHUT01011628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgtgta ggaggctggg
       61 taagtctgat gtgaaatctc accgctcaac ggtgaaactg cattggatac tgcctggcta
      121 gagagcttga gggggaatcg gaattcttgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gattcctgga aagcgtctga ctctgaaaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011629
VERSION     KHUT01011629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           91 g           43 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa aggatgcgta ggcggtctgc
       61 caagtctgat gtgaaagccc ggggctcaac cccggaacgg cattggatac tggcaggctg
      121 gaggctggaa gaggttactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaat ggcgaaagca agtaactggg acagacctga cgctgaggca tgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011630
VERSION     KHUT01011630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgata
       61 caagtcaggg gtgaaatccc agagcttaac tctggaactg cctttgaaac tgtgttgcta
      121 gagtattgga ggggagcggg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg ccgctctgga caattactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011631
VERSION     KHUT01011631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           81 g           50 t
ORIGIN      
        1 tacagaagcc ccaagcatta cccggaatca ctgggcgtaa agggtgcgta ggcggttata
       61 ttagtcgggt gttaaatcct ggggctcaac ctcagaatcg cattcgaaac ggtataacta
      121 ggagaagtga gaggtgtgcg gaactcaagg tgtaggggtg aaatccgttg atatcttggg
      181 gaacaccaaa tgcgaaggca gcacactggc actctctcga cgctgaggca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01011632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011632
VERSION     KHUT01011632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 aaagtcgggt gtgaaatccc agggctcaac cctggaagtg cactcgaaac tgcctcgcta
      121 gagttctgga gaggaaggtg gagtgcttgg tgtagaggtg aaattcgtag atatcaagcg
      181 gaacaccggt ggcgaaggcg accttctgga cagaaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011633
VERSION     KHUT01011633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          100 g           44 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtggcc
       61 caagtctggt gtgaaatctc ccggctcaac cgggagggtg cgccggaaac tgggttgctg
      121 gagtgtcgga gaggcaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggtgtagacg gcctgctgga cgaccactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011634
VERSION     KHUT01011634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           64 c          103 g           33 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactga ctgggcgtac agcgcacgca ggcggtcacg
       61 ggcgtcggtg ctgaaagcgc gccgcttaac ggcgcaaggc gtgccgatac gccgtgactg
      121 gagccatgca gaggtccggg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa ggggaagcca ccggactggg catcggctga cgctcaggtg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011635
VERSION     KHUT01011635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggtcga
       61 taagttgggg gtgaaagccc agggctcaac cttggaattg ccttcaaaac tatcgaccta
      121 gagtatggca gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011636
VERSION     KHUT01011636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggattgt
       61 taagtcagag gtgaaagccc agggctcaac cctggaattg cctttgatac tggcaatctt
      121 gagaccggaa gaggttagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctgactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011637
VERSION     KHUT01011637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtaagg
       61 tatgtcgggt gtgaaagccc tcagcttaac tggggaagtg cgcccgaaac taccttgctg
      121 gagtgtcgga gaggaaggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga cgaacactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011638
VERSION     KHUT01011638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c           90 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggccgttgtg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaactg cgcccgagac tgcacgactt
      121 gaatgcggga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaagcg gctttctggc ccgacattga cgctcatgta cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01011639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011639
VERSION     KHUT01011639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           92 g           43 t
ORIGIN      
        1 tacgtaggga gcgagcgttc tccggattta ctgggcgtaa agggtccgca ggcgggtgtc
       61 caagttccag ctgacagtcc tcggcttaac cgaggaaggc gttggaaaac tggtcacctt
      121 gagacatcga gaggcagagg gaattccgtg tggagcagtg aaatgcgtag agatgcggaa
      181 gaacaccaga ggcgaaggcg ctctgctggc gatgctctga cgctgaggga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011640
VERSION     KHUT01011640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcgcgta ggtggttatg
       61 taagttacat gtgaaagccc cgggcttaac ctgggaactg catgtaatac tgtatgacta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011641
VERSION     KHUT01011641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           94 g           40 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggagtta ctgggcgtaa agcgcacgta ggcggtccat
       61 cgcgttggtg ctgacagccg tcggcttcac tgacggaggc gtatcaaaac ggatggacta
      121 gaggctcaga gaggcagacg gaattccggg tggagcggtg aaatgcgtag agatccggaa
      181 gaacaccaaa ggcgaaggca gtctgctggc tgagcactga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01011642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011642
VERSION     KHUT01011642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagcgtgta ggcggttctc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tggagagctg
      121 gagtgtggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgcagacg gctctctgga ccattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011643
VERSION     KHUT01011643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgctcgta ggcggatatt
       61 taagtcgatt gtgaaagccc tgggcttaac ctgggaactg cagtcgatac tgggtatcta
      121 gagtatggta gaggatagtg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctgga ccaatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011644
VERSION     KHUT01011644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           84 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaagg
       61 aaagtcagat gtgaaatgcc ggagctcaac tccggaactg catttgaaac ttcctttctt
      121 gagggtaggc ggagataatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg attatctagc ttattcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011645
VERSION     KHUT01011645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c          100 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcccct
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaattg cgtctgggac tgaggggcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011646
VERSION     KHUT01011646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           93 g           41 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcatgta ggcggcttgg
       61 taagtcggac gtgaaagccc ggggctcaac cccggaaggg cgctcgatac tgccgaactt
      121 gggtcctgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg caggtaccga cgctgagatg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01011647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011647
VERSION     KHUT01011647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           93 g           49 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcgggcttg
       61 taagtcagag gtgaaagccc acggcttaac cgtggaattg cctttgatac tgcaagcctt
      121 gagtcctgga gaggctgtcg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga tgcgtaggcg gacagctgga caggtactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011648
VERSION     KHUT01011648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           80 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcgggcctt
       61 taagtaggaa gtgaaaggtt ggagcttaac tctaacactg ctccctatac tggaggtctt
      121 gagtagcgga gaggaaaacg gaacaagttg tgtagcggtg aaatgcgttg atataacttg
      181 gaacaccaat ggcgaaagca gttttctgga cgcagactga cgctgaggtt cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01011649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011649
VERSION     KHUT01011649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaggta ggtggtccca
       61 tttgtctgag gtgaaagcct tgagcttaac tcaagaagtg ccttagaaac ggtgggacta
      121 gagtactaga gagggttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcaacctgga tagtaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011650
VERSION     KHUT01011650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          105 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtggga
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcgaac tgtcccactc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacatcggt ggcgaaggcg gcctgctggg cagtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011651
VERSION     KHUT01011651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ttgggcgtaa agcgcatgta ggtggtttgt
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tgacagactg
      121 gagtcggaca gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaatatcgat ggcgaaggca gcgacctggg tccagactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011652
VERSION     KHUT01011652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           84 g           52 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggcgtag
       61 taagttgggt gtgaaatccc tcgactcaac cgaggaatgg cgcccaaaac tgctatgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011653
VERSION     KHUT01011653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggattta ctgggcgtaa agagcacgta ggtggttctt
       61 caagtctgat gtgaaatccc tggactcaat ccaggaagtg catcggatac tgtcgaactc
      121 gagtacagga gaggacagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaacagt ggcgaaggcg gctgtctgga ctgtaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011654
VERSION     KHUT01011654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagaacgta ggcggtttgt
       61 taagtctgtt cttaaagacc agggctcaac cctgggaatg gaatggaaac tggcagactg
      121 gaggacgtca gaggaaggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg accttctggg acgatcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01011655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011655
VERSION     KHUT01011655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttttt
       61 taagttggat gtgaaagccc cgggctcaac ctgggaattg cattcaagac tggaagacta
      121 gagtacggga gaggggggta gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaataccagt ggcgaaggcg gccccctggc ccgatactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011656
VERSION     KHUT01011656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtttta
       61 caagtcagat gtgaaagccc tcggctcaac cgaggacgtg catttgaaac tgtgagactt
      121 gagtattgga gggggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg accacctggc caaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011657
VERSION     KHUT01011657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           97 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggtgggtgta
       61 caagtcggaa gtgaaatctc caggctcaac ctggaggctg ctcccgaaac tgtacgcctg
      121 gggactggta gaggctggta gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccaggtccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011658
VERSION     KHUT01011658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcttac
       61 taagtctgat gtgaaatccc agggcttaac cctggaactg catcggatac tggtgagcta
      121 gaggacgaga gagggtgatg gaattccagg tgtaggggtg aaatccgtag atatctggag
      181 gaacatcggt ggcgaaggcg gtcacctggc tcgtatctga cgctgagacg caaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011659
VERSION     KHUT01011659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           87 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatga ctgggcgtaa agggtacgta ggtggctatt
       61 caagttagtg gtgaaattcc ggggcttaac tccggcgcta ccactaagac tgtttagctt
      121 gagtgcagga ggggtaagtg gaattcctag tgtagcggtg gaatgcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg acttactgga ctgtaactga cactgaagta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011660
VERSION     KHUT01011660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgaaaggt gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcgggtttg
       61 taagtcgatt gtgaaagccc tgggctcaac ctaggaattg cagtcgaaac tgcagatctt
      121 gaatgcctga gaggatgggg gaattcctgg tggagaagtg aaattcgtag atatcaggag
      181 gaacacctga ggcgaaggcg cctgtctggc ggtgcattga cgctgaggtg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01011661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011661
VERSION     KHUT01011661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcgg
       61 ccagtcgggg gtgaaatctt tcggcccaac cggaaaaggg cctccgatac tgccgatctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011662
VERSION     KHUT01011662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           39 c           90 g           57 t
ORIGIN      
        1 tacgtaggat gcgagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtaaat
       61 tgtgtctgct attaaagacc ggggcttaac ctcgggagtg tagcggaaac tggtttgcta
      121 gaggttagta gagggaatcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg gattcctggg ctaatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011663
VERSION     KHUT01011663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacgtagacc acaagcgtta tccggattta ttgggcgtaa agcgcatgta ggaggttcgg
       61 tgcgtctgtt gttaaagccc accgcccaac ggtggaaatg cagcggatac gatcgaactg
      121 gaggatgtga gaggtgcatg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca gtgcactggt acattcctga ctctgagatg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01011664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011664
VERSION     KHUT01011664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           87 g           49 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agcgcgcgta ggcggggtcg
       61 caagtcggtt gtgaaatccc tcagctcaac tgaggacctg catccgatac tgcgactctt
      121 gagcacgaaa ggggagagcg gaacttctgg tgaagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg gctctctggt tcgtttctga cgctgaggcg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011665
VERSION     KHUT01011665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           91 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgagcgta ggcggtcgcg
       61 caagtcggat gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tacgcgactt
      121 gagtatcgga gagggtgacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtcacctgga cgacaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011666
VERSION     KHUT01011666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           97 g           39 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggtggatcac
       61 caagtctgat gtgaaagccc ccggctcaac cgggggagga cgttggaaac tggtggacta
      121 gagtccgaca gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacgccaaa cgcgaaagca gccggctggg tcgagactga cactgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011667
VERSION     KHUT01011667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           62 c          108 g           31 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcgggacgg
       61 gaagtcggga gtgaaagccc ggggctcaac cccgggactg cttccgagac tcccgacctg
      121 gggaccggta ggggcgggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gcccgctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011668
VERSION     KHUT01011668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggttatc
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaatgg catacgatac tggatgacta
      121 gagtctggta gagggtggtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gccacctgga ctgagactga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011669
VERSION     KHUT01011669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtgact
       61 caagtcagag gtgaaagccc ggggctcaac cccggaactg cctttgaaac taggtgacta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011670
VERSION     KHUT01011670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           94 g           41 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggccaac
       61 caagttggat gtgaaagccc tgggctcaac ccaggacgtg cacccaaaac tggttggctt
      121 gagtcccgga gggggccaga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaataccagt ggcgaaggcg ctggtctggc cgggcactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011671
VERSION     KHUT01011671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           58 c           93 g           41 t
ORIGIN      
        1 tacgaggggc ccgagcgtta atcagaatca ctgggcttaa agcgtgcgca ggcggtccag
       61 caagcgtctt gtgaaatccc ccggctcaac cggggaagtg cagggcgaac tgctggactt
      121 gaggcaggta ggggctgatg gaaccatgag tggagcggtg aaatgcgtag atattcatgg
      181 gaacgccgat ggggaagccg atcagctggg cctgtcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011672
VERSION     KHUT01011672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           90 g           54 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctttc
       61 caggtctgat gtgaaatctc acggctcaac cgtgaaattg cgttggaaac cggaaagctt
      121 gagtgtgcta ggggtgtgtg gaacttcctg tggagcggtg aaatgtgttg atatgggaag
      181 gaacgccggt ggcgaaagcg acacactggg gcataactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011673
VERSION     KHUT01011673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggattac
       61 taagtcgggg gtgaaatccc ggggctcaac cccggaactg ccttcgatac tggtagtcta
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011674
VERSION     KHUT01011674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           85 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcacgg
       61 aaagttggat gtgaaagccc tcggctcaac cgaggaactg cgtccaaaac ttccgtgctt
      121 gagtgaggca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg cctcaactga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011675
VERSION     KHUT01011675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agagtacgta ggcggccatg
       61 taggtcaggt gtgaaatccc tcggctcaac cgaggaactg cgcttgaaac cacatggctt
      121 gagggagata gaggttagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccagt ggcgaaggcg gctaactggg tctcttctga cgctgaggta cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011676
VERSION     KHUT01011676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           51 t
ORIGIN      
        1 gacgagccac ccaaacgtta ttcggaatta ctgggcttaa agcgtgcgta ggcggcctac
       61 aaagttgggt gtgaaatccc ttggcttaac caaggaattg cgcccaaaac tagtgggctc
      121 gagggagata gaggtaagcg gaacggatgg tggagcggtg aaatgtgttg atatcatccg
      181 gaacaccggt ggcgaaggcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011677
VERSION     KHUT01011677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           50 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgcgctg
       61 taagtcgggt gtgaaatccc tcagcttaac tgaggaactg cgcccgatac tgcagtgctg
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011678
VERSION     KHUT01011678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtcact
       61 taagtctgat gtgaaagccc agggctcaac cctggaactg cattggatac tgggtgactt
      121 gagtacggga gagggaagcg gaattccgag tgtaggggtg aaatccgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011679
VERSION     KHUT01011679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagttagat gtgaaatccc tgggcttaac ctaggaactg catctgatac tggatggctg
      121 gagtgtggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ccaacactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011680
VERSION     KHUT01011680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggtcttt
       61 taggccggct gtgaaatccc atggctcaac catggaagtg cagccggaac cgggagacta
      121 gagtcctgac gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg aagcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011681
VERSION     KHUT01011681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           87 g           58 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccgtcgtt
       61 taagcgtgtt gtgaaatgcc gcggctcaac cgtggcactg cagcgcgaac tggatgactt
      121 gagttcgcgg gaggtaggcg gaattcgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gcttaccgta gcgttactga cgctgaagct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01011682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011682
VERSION     KHUT01011682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           85 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaaata ctgggcgtaa agcgaacgta ggcggtctag
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cccttgaaac tactaaacta
      121 gaatcctgga gaggcgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcgctgga caggtattga cgctgaggtt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011683
VERSION     KHUT01011683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           96 g           43 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgta ggcggagggg
       61 taggtctgat gtaacaacgc tcggctcaac tgagcgcggc cattggaaac cgcccgacta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg cgatgattga cgctgaggga cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011684
VERSION     KHUT01011684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 gacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggttttt
       61 caagtcgggc gtgaaagccc cgggctcaac ccgggatgtg cgttcgatac tggatgacta
      121 gagtgcagga gagggtggtg gaattcctag tgtagaggtg aaattcgcag atattaggaa
      181 gaacaccagt ggcgaaggcg gccacctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011685
VERSION     KHUT01011685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 tacgaactgc gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggctttt
       61 caggtccgat gtgaaatccc acagctcaac tgtggaatgg cattggaaac cggggagctc
      121 gagtgggcta gaggtacacg gaactttcgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg gtgtactggg gcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011686
VERSION     KHUT01011686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           91 g           51 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggttatt
       61 taagtcagat gtgaaagccc ttggctcaac tgaggaagtg catctgagac tgaatagcta
      121 gagtgcgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggt tcgatactga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011687
VERSION     KHUT01011687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           84 g           52 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agagtacgta ggcggttagg
       61 taagacacgg gttaaatctc cactctcaaa gtggaaaccg cccatgtgac tgcctgactc
      121 gtcggtagca ggggcaagcg gaattgtcgg tgtaggagtg aaatccgttg atatcgacaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctatttgaga cgctgaggta cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011688
VERSION     KHUT01011688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           88 g           48 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccttt
       61 caagtcaggt gtgaaatccc acagcccaac tgtggaacgg cgcttgaaac tgtttggcta
      121 gagtcagcta gaggtcggcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaagcg gccgactgga gctgttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011689
VERSION     KHUT01011689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacgagaggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggatgcg
       61 caagtgcttc gtgaaatccc ccgtctcaac cggggaactg cggggcagac tgcgtgtctt
      121 gaggcaagta gaggtcgccg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgcctga ggtgaagacg ggcgactggg cttgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011690
VERSION     KHUT01011690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           51 t
ORIGIN      
        1 tacagaggct ccaagcgtta ggcggaatca ctgggcttaa agcgtgcgta ggcggctcat
       61 caagtgtctt gtgaaagccc tcggctcaac cgaggaattg cttggcatac tggtgagctt
      121 gaatcatcta gaggcaagtg gaactgatgg tggagcggtg aaatgcgtag atatcatcag
      181 gaacgccgaa ggcgaaagca gcttgctggg gatgtattga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011691
VERSION     KHUT01011691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacgagaggc ccaagcgtta gacggaatca ctgggcttaa agcgtgcgta ggcggatgcg
       61 taagcgtgtt gtgaaatccc acagctcaac tgtggaactg cgatgcgaac tgtgtatctt
      121 gaggaaggta ggggtcagtg gaacgttcgg tggagcggtg aaatgcgtag atatcgaacg
      181 gaacgccaat ggtgaagaca actgactggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011692
VERSION     KHUT01011692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           87 g           51 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggtatca ctgggcttaa agcgtgcgta ggcggcttag
       61 caggtgaggt gtgaaatccc tcggctcaac cgaggaattg cgcttcaaac cgctaggctt
      121 gagggagaca ggggtgattg gaactgttgg tggagcggtg aaatgcgttg atatcaacag
      181 gaacaccggt ggcgaaagcg aatcactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011693
VERSION     KHUT01011693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggttcg
       61 tgtgtcgatt gtgaaagccc cgggctcaac ctgggaattg cagtcgaaac tgcggatctt
      121 gaatacctga gagggtgggg gaattcctgg tggagaagtg aaattcgtag atatcaggag
      181 gaacacctga ggcgaaggcg cctgcctggc agtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011694
VERSION     KHUT01011694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           87 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtctct
       61 tatgttcggt gtgaaagccc tgggcccaac ccaggaagtg cgccgaaaac tgagagactt
      121 gagtacgaga gaggaggacg gaattcctag tgtaggggtg aaatccgtag atattaggag
      181 gaacaccaat tgcgaaagca gttctctgga tcgatactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011695
VERSION     KHUT01011695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctatc
       61 caagttgggg gtgaaatccc tgggcttaac ccaggaactg ccttcaaaac tggatggcta
      121 gagtccgaga gaggtgagtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggtactga cgctgaggtg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011696
VERSION     KHUT01011696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           65 c          114 g           27 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agggcgcgca ggcggcccga
       61 cgcgccgggg gtgaaaggcg gtggctcaac caccggacgg cccccgggac ggtcgggctg
      121 gagggtggca gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agaccgggag
      181 gaacacccgc ggcgaaggcg gccggctggg ccaaccctga cgctgaggcg cgaaggccgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011697
VERSION     KHUT01011697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggacga
       61 taagtcaggg gtgaaagccc aaggctcaac cttggaattg cctttgatac tgtcgttctt
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011698
VERSION     KHUT01011698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           90 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggagcgca ggtggtttct
       61 taagtctgat gtgaaagccc acggctcaac cgtggagggt cattggaaac tggggaactt
      121 gagtacagaa gaggaaagtg gaattccaag tgtagcggtg gaatgcgtag atatttggag
      181 gaacaccagt ggcgaaggcg actttctggt ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011699
VERSION     KHUT01011699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgatt
       61 ctagtcaggt gtgaaagccc tgggctcaac ccgggaactg cacttgatac tggattgcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011700
VERSION     KHUT01011700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           94 g           51 t
ORIGIN      
        1 tacgtaggtg tcaaacgttg tccggattta ttgggcgtaa agggcccgca ggcggctttg
       61 taagttctaa gtgaaatctc ccggctcaac tgggaggggt cttggaaaac tgcaaggctt
      121 gaggcaggga gagggatgtg gaattccagg tgtagcggtg gaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctgagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011701
VERSION     KHUT01011701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcgt
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tggcggactt
      121 gagtacggga gggggctgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcagcctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011702
VERSION     KHUT01011702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttttt
       61 taagtctggt gttaaatact ggcgctcaac gccagcatgg cattggaaac tggaagacta
      121 gagtgcggta gaggtcacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtgactggg ccgtaactga cgctgaggaa cgaaagccag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01011703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011703
VERSION     KHUT01011703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           82 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagtatgta ggcggacatt
       61 taagtagaaa gtgaaaggtt ggagctcaac tccaacattg cttcctatac tgggtgtctt
      121 gagtatcgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctttctgga cgaacactga cgctgagata cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011704
VERSION     KHUT01011704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           53 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggcttgg
       61 taagtcaggt gtgaaagccc agggcttaac cctggaagtg catttgaaac tgccgagctt
      121 gagtgtggga gaggatagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gctatctggc ccaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011705
VERSION     KHUT01011705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggctgt
       61 taagtcagag gtgaaatccc ggggctcaac cccggaactg cctttgatac tggcagcctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011706
VERSION     KHUT01011706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggtctgg
       61 taagtggggt gtgaaatccc tcggcttaac cgaggaattg cgctccaaac tgccaggctt
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccaga ggcgaaggcg gctcactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011707
VERSION     KHUT01011707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           92 g           46 t
ORIGIN      
        1 tacggaggat gcgagcgttg ttcggaatca ctgggcttaa agcgcgtgca ggcggaatgg
       61 aaggtgtctt gtgaaatccc ccggctcaac cggggaactg cttggcaaac caccattctt
      121 gaggcaggta gaggcgactg gaaccttcgg tggagaggtg aaattcgtag atatcgaagg
      181 gaacgccggt ggcgaaagcg agtcgctggg cctgtcctga cgctgagacg cgaaagcgta
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011708
VERSION     KHUT01011708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttta
       61 tgggtgtgat gtgaaagccc cgggcttaac ccgggaagtg catcgcaaac tattgaactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011709
VERSION     KHUT01011709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           80 g           51 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtttcttgc
       61 cacgtctggc gtaaaatttc agtgctcaac actgaatacg tgccggatac gagcgagata
      121 gagtcattca gaggtatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaggca ggatactggg aatgcactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011710
VERSION     KHUT01011710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           85 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtttta
       61 taagtcagtg gtgaaatccg gtcgcttaac gatcgatgtg ccattgatac tgtaggactt
      121 ggatatgttg gaggttgccg gaatgggtgg tgtagcggtg aaatgcatag agatcaccca
      181 gaacgccaat tgcgaaggca ggtgactacg acattatcga cactgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011711
VERSION     KHUT01011711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta gccgggccga
       61 caagtcagat gtgaaatccg cgggcttaac ccgcgaactg catttgaaac tgttggtctt
      121 gagtatcgga gaggtcatcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gatgactgga cgacaactga cggtgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011712
VERSION     KHUT01011712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggccctg
       61 taagtcagga gtgaaatccc ggagctcaac tccggaattg cttttgaaac tacaggacta
      121 gagtatgtta gaggatggcg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg acataactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011713
VERSION     KHUT01011713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           60 c          107 g           40 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccatg
       61 ttggcctggc gtgaaagccc tcggcttaac cgaggaacgt cgtcggggac cgcatggctt
      121 gaggatggca ggggctggtg gaatgcccgg tgtagtggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccagctggg ccattcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011714
VERSION     KHUT01011714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcaggg gtgaaatctt tcagcttaac tggaaaacag cctctgatac tggccgtctt
      121 gagtggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011715
VERSION     KHUT01011715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          102 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggccgtt
       61 caagtcctgt gtgaaaggcc ccggcttaac cggggagctg catgggaaac tggacggctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtaactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011716
VERSION     KHUT01011716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 tgagttaagt gtgaaagccc aaggctcaac cttggaactg catttaacac tggcaggcta
      121 gagatcgcaa gaggatggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaatatcaat ggcgaaggca gccatctggg gcgatactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011717
VERSION     KHUT01011717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           90 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgat
       61 taagttagct gtgaaattcc tgggcttaac ctgggctgga cgactaagac tgattgactg
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc cagatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011718
VERSION     KHUT01011718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           36 c           83 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtccgta ggtggttaga
       61 taagtcaaat gtgaaagact cgggcttaac ctgagaatgg catttgatac tgtttggcta
      121 gagtattgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga caaatactga cactgaggga cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011719
VERSION     KHUT01011719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           92 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 caagtcgaat gtgaaatccc ccggctcaac tggggagcgt cattcgatac tgatcgactt
      121 gaaggcagga gagggtagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011720
VERSION     KHUT01011720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           96 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttaa
       61 tgggtgtgat gtgaaagccc cgagcttaac ttgggaagtg catcgcaaac tgttgagctg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011721
VERSION     KHUT01011721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 taagtctaac gtgaaatctc ggagcttaac tccgaaattg cgtcggatac tgcgttgcta
      121 gaggattgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg caactcctga cactgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011722
VERSION     KHUT01011722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 taagtcagat gtgaaatccc cgggcttaac tcgggacgtg catttgaaac tgcgaggcta
      121 gagtgcagta gaggatggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg ctgcaactga cgctgaggcg caaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011723
VERSION     KHUT01011723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           91 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccaat
       61 taagtcagat gtgaaatccc cgggcttaac ctgggaactg catttgagac tggatggctt
      121 gaggaccgga gagggtagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actacctgga cggcacctga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011724
VERSION     KHUT01011724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           86 g           57 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccgtcttt
       61 taagcgtgtt gtgaaatact gtcgctcaac gacagaggtg cagcgcgaac tgggagactt
      121 gagtgcgcgg aatgcaggcg gaattcgtcg tgtagcggtg aaatgcttag atatgacgaa
      181 gaactccgat tgcgaaggca gcttgcagta gcgtaactga cgctgaagct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01011725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011725
VERSION     KHUT01011725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           36 c           76 g           69 t
ORIGIN      
        1 tacggagggt ccgagcgtta tccggaatta ttgggtttaa agggtccgta ggcggttttg
       61 taagtcagtg gtgaaatctc ctagcttaac taggaaattg ccgttgatac tgtgagactt
      121 gaatcattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact agtgtattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011726
VERSION     KHUT01011726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           37 c           88 g           59 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggtggtttta
       61 taagttaggt gtgaaagtcc tgggcttaac ctggggattg cgcctgatac tgtagagcta
      121 gagtactgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagtgactga cactgaggca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011727
VERSION     KHUT01011727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggtggtggtt
       61 taagtcagat gtgaaagccc tgggctcaac ctgggaactg catttgatac tggatcacta
      121 gagtgtggta gaggatagcg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacatccgt ggcgaaggcg gctatctggg ccaacactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011728
VERSION     KHUT01011728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           92 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtttgg
       61 taagtcgaat gtgaaagccc agggctcaac cttggaagtg catccgaaac cactaaacta
      121 gagtcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011729
VERSION     KHUT01011729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           98 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcgatt
       61 taagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgagac tgagtcgctt
      121 gagtgccgga gagggtggcg gaattcccca agtagaggtg aaattcgtag agatggggag
      181 gaacaccgga ggcgaaggcg gccacctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011730
VERSION     KHUT01011730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           75 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggcggactta
       61 taagtcagtg gtgaaatctc cgggcttaac ccggaaactg ccattgatac tgtaggtcta
      121 gagtacattt gctgtgggcg gaatatgaca tgtagtggtg aaatacttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa atgtaactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01011731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011731
VERSION     KHUT01011731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggttgg
       61 gtagtcagtg gtgaaatact ttcgcttaac gaaggaactg ccattgatac tctcaatctt
      121 gaatacagtg gaggtgggcg gaatgcgtca tgtagtggtg aaatacttag atatgacgca
      181 gaacaccaat tgcgaaggca gcttgctaca ctgttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011732
VERSION     KHUT01011732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           91 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgac
       61 taagttatct gtgaaatccc tgggcttaac ctgggcctgt cagataagac tggttgactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcggt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011733
VERSION     KHUT01011733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           85 g           50 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccatg
       61 tgtgtctggt gtgaaatctc ggggcttaac tccgaaattg cgccggaaac tacatggcta
      121 gagtactgga gagggtagcg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaacaccaat ggcgaaggca gctacctgga cagttactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01011734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011734
VERSION     KHUT01011734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           37 c           76 g           62 t
ORIGIN      
        1 tacaggggat gcaagtgtta ttcggaatca ttgggcgtaa agcgtctgta ggtggacaat
       61 taagtctttt gtcaaatctt tgggcttaac ctaaagtgtg caaaagaaac tgtttgtcta
      121 gagtctgtta gaggtaaagg gaatttccag tggagcggtg aaatgcgtag atattggaag
      181 gaacaccaaa ggcgaaggca ctttactggg tcagtactga cactgagaga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011735
VERSION     KHUT01011735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggtctg
       61 caagtcagat gtgaaatccg tgggcttaac ccacgaactg catttgaaac tgtagatctt
      121 gagtatcgga gaggtaatcg gaattccttg tgtagcggtg aaatgcgtag atataaggaa
      181 gaacaccagt ggcgaaggcg gattactgga cgacaactga cggtgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011736
VERSION     KHUT01011736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           56 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgcgta gacggcatat
       61 taagtctgtt gttaaatccc tcggcttaac cgaggatatg cggcggatac tgatgtgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011737
VERSION     KHUT01011737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggtggctatg
       61 taagtcaggt gtgaaatccc ggagcttaac ttcggacttg cgcttgatac tgtgtagcta
      121 gagtttagta gagggcagtg gaatttccgg agtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actgcctgga cgaagactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011738
VERSION     KHUT01011738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           86 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggcgtaa agagttcgta ggcggtatgt
       61 aaagtcaggt gttaaaggca ggggctcaac ttctgtacgg cacttgatac ttgcaaacta
      121 gaatgcggta gaggtaaagg gaattcctgg tgtagcggtg aaatgcttag atatcaggag
      181 gaacatcggt ggtgtaagcg ctttactggg ccgtaattga cgctgaggaa cgaaagccag
      241 ggtagcaaat ggg
//
LOCUS       KHUT01011739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011739
VERSION     KHUT01011739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcgcgta ggcggtaaga
       61 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tgttttactt
      121 gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgcag agatatggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011740
VERSION     KHUT01011740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           81 g           59 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcgctt
       61 taagtcagtg gtgaaatacg gcagctcaac tgtcgcagtg cccttgatac tgaagtgctt
      121 gaatactgtt gaagatggcg gaatgagaca agtagcggtg aaatgcatag atatgtctca
      181 gaacaccgat tgcgaaggca gctgtctaag cagtgattga cgctgatgca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01011741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011741
VERSION     KHUT01011741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgca ggcggctaga
       61 caagtcagag gtgaaatccc agggctcaac cctggaactg cctttgaaac tgtctggcta
      121 gagttcggga gagggtagtg gaattcctag tgtagcagtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctacctggc ccgatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011742
VERSION     KHUT01011742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           99 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agggcaagta ggtggctgga
       61 tgcgtggggt ttgaaaacac ctggcttaac tgggtgagtg ggccccaaac ggtcgagctt
      121 gaggtaggga gagggatgcg gaattcctgg tgtagtggtg gaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaagcg gcatcctggc cctgacctga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011743
VERSION     KHUT01011743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           84 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcgggaata
       61 taagtcagat gtgaaattcc gaggctcaac ttcggagctg catttgaaac tatatacctt
      121 gaggattggc ggagagaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaagcg gttctctagc tcactcctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01011744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011744
VERSION     KHUT01011744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           79 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaactg ccattgatac tatctgtctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacatcgat tgcgaaggca gctcgctacg caaatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011745
VERSION     KHUT01011745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           95 g           39 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtcact
       61 caagtctgat gtgaaatctc tccgctcaac ggggagcagc cattggaaac tgggcgactg
      121 gagtctgaca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg tcagtactga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011746
VERSION     KHUT01011746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          107 g           38 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggttctg
       61 cacgtcgggt gtgaaagccc cccgctcaac gggggagggt cattcgaaac ggcaggactg
      121 gaggcaggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccgactgga cctgtactga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011747
VERSION     KHUT01011747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagttagag gtgaaagccc ggagctcaac tccggaactg cctttaaaac tgcatcgcta
      121 gaattgtgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011748
VERSION     KHUT01011748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 cacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggcctgg
       61 agggtggggt gtgaaatccc tcggctcaac cgaggaatgg cgctccaaac cgtcaggctt
      121 gagggagata gaggtgagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaacaccggt ggcgaaagcg gctcactgga tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011749
VERSION     KHUT01011749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 caagtcgggt gtgaaatccc caggctcaac ctgggaatgg catccgaaac tgcttcgcta
      121 gagtctcgga gaggaaggca gaattcccag tgtaggggtg aaatccgtag atactgggag
      181 gaatacccgt agcgaaggcg gccttctgga cgatgactga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011750
VERSION     KHUT01011750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttatt
       61 taagctagat gtgaaagccc agggctcaac cttggaactg catttagaac tgggtaacta
      121 gagtacagca gaggggtgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg acactctggg ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011751
VERSION     KHUT01011751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           45 c           97 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggcgtaa agggcatgta ggtggtttgt
       61 taagtctggc gttaaaggct ggggctcaac cctggtatgg cgttggatac tggcaggctt
      121 gacggttgga gaggcgagtg gaattgccag tgtagcggtg gaatgcgtag atactggtag
      181 gaacacctat tgcgaaggca gctcgctgga caactcgtga cgctgaggtg cgaaagtgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011752
VERSION     KHUT01011752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          104 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttcc
       61 taagcgggtg gtgaaagctc ggggctcaac ctcgagtcgg ccatgcgaac tgggaagctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011753
VERSION     KHUT01011753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           94 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggtttgt
       61 taagtcgggt gtgaaatccc ggggctcaac cccggaagtg cattcgaaac tggcagactt
      121 gagtcccgga gaggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatccgt ggcgaaggcg gccatctgga cggtgactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011754
VERSION     KHUT01011754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ctcggaatca ctgggcataa agggcgtcta ggcgggttga
       61 caagtcggct gtgaaatccc tcggctcaac cgaggaactg cagccgatac tgtcggtctt
      121 gagggcgaga ggggagagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggc tcgttcctga cgctgaagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011755
VERSION     KHUT01011755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           95 g           47 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggccgtt
       61 cacgtcggat gtgaaagctt gaggctcaac ctcaagaggc catctgatac ggaacggcta
      121 gaggcaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg ccttgcctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01011756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011756
VERSION     KHUT01011756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           98 g           42 t
ORIGIN      
        1 gacggagggt gcgaacgttg ttcggaatca ctgggcataa agagcacgta ggcggttcgc
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaattg ctcctgatac tggcgggcta
      121 gaggacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtaactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011757
VERSION     KHUT01011757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagtccgta ggcggcgcga
       61 caagtgaggt gtgaaagccc tcggctcaac cgaggaattg cgcttcaaac tgtcgtgctt
      121 gaggaaccta gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg ggtttactga cgctgaggga cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011758
VERSION     KHUT01011758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggtagaa
       61 taagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac tgttcaacta
      121 gagttcagtt agggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctgatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011759
VERSION     KHUT01011759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtaaga
       61 tgtgtgtgat gtgaaagccc cgggctcaac ctgggaagtg catcgcaaac tatctaactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011760
VERSION     KHUT01011760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgtacgta ggcggcgcgt
       61 caagtgcggg gtgaaagccc acggcttacc cgtggaacag ctttgcatac tgacgtgcta
      121 gagtgcggta gaggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccgaa ggcgaaggca gctctctggg ccgttactga cgctgaggta cgaaagccag
      241 gggatcaaac ggg
//
LOCUS       KHUT01011761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011761
VERSION     KHUT01011761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           89 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggctatg
       61 caagtcggat gtgaaatccc acggctcaac catggaagtg cattcgaaac tgcatagcta
      121 gagtcctgga gaggaaggtg gaatgctagg tgtagaggtg aaattcgtag atatctagcg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctggaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011762
VERSION     KHUT01011762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcggacttc
       61 atagtcgggg gtgaaatttt gcggcttaac cgtaaaagag cctccgatac tggaagtctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga tctcttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011763
VERSION     KHUT01011763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggtatgt
       61 taagtgagtt gtgaaattcc tgggcttaac ctaggaactg caaattagac tggcgtactg
      121 gagtgcagta gagggtagaa gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011764
VERSION     KHUT01011764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           93 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggccggc
       61 taagtcaggg gtgaaatccc gccgctcaac ggtggaactg cccttgatac tggccggctc
      121 gaatgggcgc gaggcgggcg gaacgtggca tgtagcggtg aaatgcttag atatgccaca
      181 gaacaccgat tgcgaaggca gcccgccaga gccctattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011765
VERSION     KHUT01011765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           88 g           50 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggtggttgta
       61 caagtcgggt gtgaaatcct ggggcctaac ctcagaatgg cattcgaaac tgtacagctg
      121 gaggtctgga gaggtgactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaagcg agtcactgga cagaacctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011766
VERSION     KHUT01011766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          103 g           41 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgcgt
       61 taagtcggtt gtgaaagccc ggggctcaac cccggaacgg cagccgagac tggcgggctc
      121 gagaccggga gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggc ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011767
VERSION     KHUT01011767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           86 g           52 t
ORIGIN      
        1 tacgaaggcc ccgagcgtta tccggaatta ttgggcgtaa agggtgcgta ggtggttttg
       61 cgcgtctgtt gttaaatgtc acagctcaac tgtgtcaccg caatggatac ggcaagacta
      121 gaggatgcga gaggtctgtg gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca gcagactggc gcatttctga cactgaggca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01011768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011768
VERSION     KHUT01011768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgag
       61 caagtccggg gtgaaatcct tcggcttaac cggagaagtg ccttggatac tgctcgtctc
      121 gagggaggta ggggttatcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gataactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011769
VERSION     KHUT01011769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggtctgt
       61 taagtcagga gtgaaactcc gcagctcaac tgcggacctg cttttgaaac tggcggactt
      121 gagtgctcta gagggcagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actgtctggg gagcaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011770
VERSION     KHUT01011770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaagta ggtggtctca
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg cctcagaaac ggtgagactc
      121 gagtactgga gagggtcgtg gaatacccgg tgtagcggtg aaatgcgtag agatcgggtg
      181 gaacaccaga ggcgaaggcg gcgacctgga cagttactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011771
VERSION     KHUT01011771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           79 g           62 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggctgttcgg
       61 taagttagag gtgaaaggtt aacgcttaac gttaaaactg cctctgatac tgccgggcta
      121 gagtttagtt gctgtgggcg gaatgagtag tgtagcggtg aaatgcttag atattactca
      181 gaacaccgat tgcgaaggca gctcacaaag ctataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011772
VERSION     KHUT01011772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           93 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggttatg
       61 agagtgtggt gtgaaagccc cgggcttaac ctgggaagcg catcgcaaac gccataactg
      121 gagtagatga gagggtgatg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgtcaat ggcgaaggca gtcacctggc atcatactga cgctgaggcg cgagagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01011773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011773
VERSION     KHUT01011773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           88 g           49 t
ORIGIN      
        1 tacagagagg gcaagcgttg ttcggattca ctgggcataa agcgcacgta ggcgggtcgg
       61 taagtcgggt gtgaaatccc ggagctcaac tccggaactg cttccgatac ttccggccta
      121 gaggatgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggc tcatttctga cgctcaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011774
VERSION     KHUT01011774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           98 g           42 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcggatcgc
       61 caagtccggc gtgaaagctc ccggctcaac tgggagaggg cgttggaaac tggcgatctt
      121 gaatcacaca gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca accggctggg tgaggattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011775
VERSION     KHUT01011775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           93 g           46 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggttgcg
       61 taagtggaat gtgaaatccc caggcttacc ctgggacctg catcccaaac tgcgcgactt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011776
VERSION     KHUT01011776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatt
       61 taagccagac gtgaaagccc cgggcttaac ctgggaatgg cgtttgggac tggatagcta
      121 gagttcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaggactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011777
VERSION     KHUT01011777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           84 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggtttga
       61 taagtctgtt gtgaaagctc ctggcttaac tgggagaggt caacggaaac tatcaggcta
      121 gagtgcaata gaggaaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtttctgga ttgtcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011778             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011778
VERSION     KHUT01011778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           96 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggccggg
       61 cgggtcgtgg gtgaaatccc ccagcttaac tggggaactg cctacgatac cacctcggct
      121 cgagggagga aggggcgacc ggaactgtcg gtggagcggt gaaatgcgtt gatatcgaca
      181 ggaacgccga tggcgaaagc aggtcgctgg ccctcttctg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011779             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011779
VERSION     KHUT01011779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           55 c           80 g           49 t
ORIGIN      
        1 ccccggcgcc acaagtggtt atcacgatta ttgggcctaa agcgttcgta gccggtttgg
       61 taaatctctt gtgaaattgt tcggcttaac cggacagcgt gcaggagaca ctaccagact
      121 cgagaccggg aggggttaga agtattccat ggggactggt aaaatgggat aatccatgga
      181 agactaccga tggcgaaggc atctaaccag aacggatctg acggtgagga acgaaagcca
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01011780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011780
VERSION     KHUT01011780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           82 g           57 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggttttg
       61 ttagtctttc cttaaattct tcggcttaac cggggacatg ggaaggaaac ggcataacta
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca attcactggc gcactcctga cgctgagaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01011781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011781
VERSION     KHUT01011781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c           99 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggcgcgca ggcggttcgt
       61 taagtccgag gtgaaatctc ctggctcaac tgggaggggt cttcggatac tggcgggctt
      121 gaggtgagca gaggtgagcg gaattctggg tgtagtggtg aaatgcgtag atatccagag
      181 gaacaccagt ggcgaaggcg gctcactggg ctccacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011782
VERSION     KHUT01011782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           91 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatga ttgggcgtaa agggtgcgca ggcggtttgc
       61 taagacggat gtgaaagccc cgggctcaac ctgggaatgg catacgtgac tggcaaacta
      121 gaatatggca gagggaggtg gaattccatg tgtagcagtg aaatgcgtag agatatggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011783
VERSION     KHUT01011783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggctttt
       61 caagttgggt gtgaaagccc ggggcttaac cccggaaatg cgcccaagac tgtttggcta
      121 gagaccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011784
VERSION     KHUT01011784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           95 g           43 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctcg
       61 tttgtgtggg gtgaaagcct tgggctcaac ccaagaagtg ccccgcaaac ggcgagacta
      121 gaggactgga gaggctagcg gaatacccgg tgtagcggtg aaatgcgtag agatcgggtg
      181 gaacaccaga ggcgaaggcg gctagctgga cagttactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011785
VERSION     KHUT01011785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtttgg
       61 taagtctgtc gtgaaaaccc agggctcaac cccgggcgtg cggtggatac tgccaggcta
      121 gagggtggta gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccattcctga cgctgagacg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01011786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011786
VERSION     KHUT01011786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 tgcgtcggct gtgaaaactc agggctcaac cctgagcctg cagccgatac gagcagacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011787
VERSION     KHUT01011787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           84 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttg
       61 taagtcagtg ttgaaatatg gcagcttaac tgtcggactg gcattgatac tgcagagctt
      121 gagtacagtt gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gcttgctaag ctgtaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01011788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011788
VERSION     KHUT01011788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           90 g           52 t
ORIGIN      
        1 tacagggggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctaa
       61 taagtcagat gtgaaagcct ggggcttaac tccagaagtg catttgaaac tgttaggcta
      121 gagtgatgta gaggatggcg gaatacccag tgtagaggtg aaattcgtag agattgggta
      181 gaacaccggt ggcgaaggcg gccatctggg cattaactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011789
VERSION     KHUT01011789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           83 g           58 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggattta ctgggcgtaa agcgtgcgta ggtggtttcc
       61 taagttagaa gtgaaagccc agggcttaac cctggaattg cttttaagac tgggtcacta
      121 gaatatagga gaggtagatg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaat ggcgaaagca gtctactgga ctattattga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011790
VERSION     KHUT01011790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           98 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgcgc
       61 acagtcaggc gtgaaattcc cgggcttaac ctgggggctg cgcttgatac gtgcgtgcta
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011791
VERSION     KHUT01011791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtttg
       61 taagtcggaa gtgaaatctc actgctcaac ggtggggctg cttctgatac tgcaaacctt
      121 gagaccggta gaggttggta gaattgcagg tgtagcggtg gaatgcgtag agatctgcaa
      181 gaatacccgt ggcgaaggcg gccaactggg ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011792
VERSION     KHUT01011792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           87 g           51 t
ORIGIN      
        1 gacataggtg gcaagcgtta ttcggaatta ctaggcgtaa agcgagtgta ggcggactct
       61 taagttcgac gttaaatctc ccggcttaac tgggagcggt cgtcgaatac tgagagcctc
      121 gagtggggta gggggcagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca gctgcctggg cctttactga cgctaagact cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011793
VERSION     KHUT01011793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 tacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaagccc ggagctcaac tccggaattg cattcgaaac tgcctcgctc
      121 gagtcccgga gagggtggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011794
VERSION     KHUT01011794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggattta ctgggcgtaa agcgcatgta ggcgggacga
       61 taagtcgggt gtgaaatccc tgggctcaac ctaggaactg catccgaaac tgccgttctg
      121 gagtagtgga gaggaaagcg gaattccggg tgtagaggtg aaattcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gctttctgga cactaactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011795
VERSION     KHUT01011795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacgtatggt gcaagcgttg ttcggaatca ctgggcgtaa agagcacgca ggcggactat
       61 taagtcggga gtgaaaacta tgggctcaac ccatagcctg ctcctgaaac tggtggtctg
      121 gagtctcgga gaggtaagcg gaattctcgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaggca gcttactgga cgatgactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011796
VERSION     KHUT01011796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          101 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgt
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcggac tggcaagctg
      121 gagcacagta gaggcaggtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctgttgctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011797
VERSION     KHUT01011797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggcggtg
       61 taagttggac gtgaaatctc ccggcttaac tgggcgaggt cgttcaatac tgcgcggcta
      121 gaggctgtca gagggcagtg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctgcctggg gcaagcctga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011798
VERSION     KHUT01011798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtccgt
       61 aaagttggat gtgaaatccc acagcttaac tgtggaactg cattcaaaac tcacggacta
      121 gagtatggaa ggggtgaggg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg cctcactggt ccattactga cgctcaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011799
VERSION     KHUT01011799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgtt
       61 cgcgtcgggt gttaaagctc agggctcaac cttgagaggc catccgatac gggacagcta
      121 gaggcaggta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggag cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01011800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011800
VERSION     KHUT01011800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           86 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtgggtaaa
       61 taagttggac gtgaaagccc cgggcttaac ctgggatggt cgtccaatac tgtttatcta
      121 gagtatggta gagggtggta gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaatatcaat ggcgaaggca gccacctggg ctaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011801
VERSION     KHUT01011801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           94 g           50 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttcat
       61 taagttggat gtgaaaactc tgggcttaac tcagagcctg cattcaaaac tgatgggctg
      121 gagttctgga ggggatagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagaaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011802             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011802
VERSION     KHUT01011802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           58 c           87 g           45 t
ORIGIN      
        1 caccggcgcc acgagtggtc atcacgatta ttgggcctaa agcgttcgta gccggtttgg
       61 taaatctctt gtgaaattgt tcggcttaac cggacagcgt gcaggggaca ctgccaagct
      121 cgagaccggg agggggtaga agtattccgt ggggaccggt aaaatgggat aatccacgga
      181 ggactaccga tggcgaaggc atctacccag aacgggtctg acggtgagga acgaaagcca
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01011803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011803
VERSION     KHUT01011803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggtgcgta ggcggacctt
       61 taagtgagat gtgaaatccc cgagcttaac ttgggggctg catttcaaac tggaggtcta
      121 gagtgcagga gaggagagtg gaattcctag tgtagaggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actctctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011804
VERSION     KHUT01011804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           90 g           53 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatta ttgggcgtaa agcgtgtgta ggctgccttg
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg catctgatac tgcatggctt
      121 gagtactgga gaggatagcg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctggc catgtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011805
VERSION     KHUT01011805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           90 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttatg
       61 taagtgagag gtgaaatccc tgggcttaac ctgggaactg cctcttatac tggatagctg
      121 gagtacaata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ttgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011806
VERSION     KHUT01011806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c          101 g           39 t
ORIGIN      
        1 aacgtagggt gcaagcgttg ctcggaatta ctgggcgtaa agggcgtgta ggcggctgtg
       61 caagtcgcgt gtgaaagccc ccggctcaac cggggaattg cgcgcgaaac tgctcggctt
      121 gaggacggta ggggacagtg gaactccagg tggagcggtg aaatgcgtag agatctggag
      181 gaacgccgga ggcgaaagcg actgtctggg ccgatcctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011807
VERSION     KHUT01011807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacgtagggt gcgaacgttg ttcggaatca ctgggcataa agcgcgcgta ggcggtctag
       61 taagtcccgt gtgaaagccc tcggcttaac tggggaattg cacgcgatac tgctgggcta
      121 gagtgcagga ggggagagcg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccagc ggcgaaagcg gctctctggt ctgtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011808
VERSION     KHUT01011808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcgggg gtgaaatcgt tcggcccaac cggacaaggg cttccgatac tgactgtctc
      121 gagggaggta ggggtcgagg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011809
VERSION     KHUT01011809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          100 g           42 t
ORIGIN      
        1 tacagagggg gccagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggccctg
       61 cgtgtcttcc gtgaaatccc tcggctcaac tgaggacgtg cggaagaaac tgcggggctc
      121 gagtccggga gaggggagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctccctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011810
VERSION     KHUT01011810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           78 g           64 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaattg
       61 taagtcagtg gtgaaatccc gtagcttaac tatggaactg ccattgatac tgctattctt
      121 gaatgtagtt gaggtaggcg gaatgtgtgg tgtagcggtg aaatgcatag atatcacaca
      181 gaacaccgat tgcgaaggca gcttactaaa ctattattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011811
VERSION     KHUT01011811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatga ttgggcgtaa agcgcgtgta ggcggccgat
       61 tatgccggtt gtgaaagccc gtggctcaac cacggaagtg cagtcggaac tgatcggcta
      121 gagtaccgaa gagggtggcg gaatgcccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccacctggg aggctactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011812
VERSION     KHUT01011812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cgcccgaaac tgccttgctt
      121 gagtaccgga gagggtagcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gctacctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011813             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011813
VERSION     KHUT01011813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           77 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ctgggcgtaa agtgtgcgta ggtggtttaa
       61 ttagttatcg gtgggaaatt ggggctcaac cccacggaag tcgataatac tattaaacta
      121 gaggatcgga gaggttaacg gaattctatg tgtagcggta aaatgctcaa atatatagag
      181 gaacaccaat tggcgaaggc agttaactat tcgactcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01011814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011814
VERSION     KHUT01011814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggcgggg
       61 taagttcggt gtgaaatctc caggctcaac ctggaaatgg cattgaatac tactctgctc
      121 gaggcttgga ggggggactg gaatgctcgg tgtagcagtg aaatgcgtag agatcgagcg
      181 gaacaccagt ggcgaaggcg agtccctgga caagtcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011815
VERSION     KHUT01011815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacgagaggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcgggtttg
       61 tatgtcggtt gtgaaagccc agggcttaac cctggaattg cagccgaaac tgcaaatctt
      121 gaatacttga tagggtggga gaattcctgg tggagcagtg aaatgcgtag agatcaggag
      181 gaataccgga ggcgaaggcg cctgcctggc aagatattga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011816             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011816
VERSION     KHUT01011816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcgcgta ggcggcgttg
       61 ttagttggag gtgaaatacc atcggctcaa ccgtggaatt gcccccaata ctgcaacgct
      121 tgagggaggt aggggcatgt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacatgctgg aactcttctg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01011817             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011817
VERSION     KHUT01011817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          102 g           43 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccttg
       61 gcagtcggct tgtgaaagcc cggggctcaa ccccggaggt gcggccgata caccgaggct
      121 tgaggccgga agaggagggt ggaataccca gtgtagaggt gaaattcgta gatattgggt
      181 ggaacaccgg tggcgaaagc ggctctctgg tccggttctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011818             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011818
VERSION     KHUT01011818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           95 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgca ggcggtgaga
       61 taagtctggg gtgaaaggcg gccgctcaac ggtcagtagg gccccggata ctgttttact
      121 cgagtgcagg cgaggccggc ggaacgggtg gtgtagcggt gaaatgcata gataccaccc
      181 agaactccga ttgcgaaggc agccggccag actgccactg acgctgaggc acgaaagcgc
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011819             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011819
VERSION     KHUT01011819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcatga
       61 taagtctgaa gtgaaagccc ggggctcaac cccgcggact gctttggaaa ctatcaggct
      121 ggagtgcagg agaggtaagt ggaattccta gtgtagcggt ggaatgcgta gatattagga
      181 ggaacaccag tggcgaaggc ggcttactgg actgtaactg acgttgaggc tcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011820
VERSION     KHUT01011820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtccgca ggcggtctgg
       61 caagttcgag gtgacagtgc gcggcttaac cgcgcaaggt ccttgaaaac tggcagactt
      121 gagggcttca gagggacccg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaaa ggcgcaggca gggtcctggg aagctactga cgctcatgga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011821             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011821
VERSION     KHUT01011821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcgggt gtgaaacctc caggctcaac ctggagtcgc cacccgatac tgctatgact
      121 tgagtctggt aggggatcac ggaattcctg gtgtagcggt gaaatgcaca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccagtactg acgctcaaga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01011822             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011822
VERSION     KHUT01011822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcctt
       61 taagttgatt gtgaaatctt cacggctcaa ccggaaaact gcggtcaaaa ctggaggact
      121 tgagattggg agagggaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agcttcctgg cccaattctg acgctgaggt gcgaaagcgt
      241 gggtagcgaa cggg
//
LOCUS       KHUT01011823             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011823
VERSION     KHUT01011823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           47 t
ORIGIN      
        1 tacggggggg gcaaacgttg ctcggaatca ctgggcgtaa agcgtgtgta ggcggtccaa
       61 tttgtcggac gtgaaagccc tgggcttaac ccaggaactg cattcgaaac ggttggactt
      121 gagtaccgga ggggtcgacg gaattcccgg tgtagtggtg aaatacgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gtcgactgga cggatactga cgctgagtac acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011824             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011824
VERSION     KHUT01011824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           84 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgagtgta ggtggctcat
       61 taagtcacat gtgaaatccc cgggcttaac ctgggaactg catgtgatac tggtggtgct
      121 agaatatgtg agagggaagt agaattccag gtgtagcggt gaaatgcgta gagatctgga
      181 ggaataccga tggcgaaggc agcttcctgg cataatattg acactgagat tcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011825
VERSION     KHUT01011825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           87 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggtgcgta ggtggcatgg
       61 taagttggat gtgaaaaccc aaggctcaac tttgggactg catctaaaac tgccaagcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt agcgaaggcg gctttctgga ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011826
VERSION     KHUT01011826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggcgagg
       61 tatgtcggat gtgaaatcct tcagcttaac tgaggaacgg cattcgaaac tacttcgctc
      121 gagtgcgaga gaggatggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaatacctgc ggcgaaggcg gccatctggt tcgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011827
VERSION     KHUT01011827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggataat
       61 caagtcaaag gtgaaagccc tgggctcaac ctgggaattg cctgtgaaac tgattgtcta
      121 gagtagagta gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctctctgga ctcatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011828             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011828
VERSION     KHUT01011828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctgt
       61 taagtcgagt gttaaatccc agggcttaac cctggactgc tcgctcgata ctagcaggct
      121 agagtgtgga agagggtgct ggaattcccg gtgtagcggt gaaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc gggcacctgg gccaacactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011829
VERSION     KHUT01011829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggatca
       61 taagtcagat gtgaaattcc atggctcaac tgtggcactg catctgaaac tatggttctt
      121 gagtgctgga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011830
VERSION     KHUT01011830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           87 g           45 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcctg
       61 caagttgggt gtgaaagccc tcggctcaac cgaggaactg cgcccaaaac tgcgggactt
      121 gagggagaca taggtgagcg gaacttaggg tggagcggtg aaatgcgttg agatcctaag
      181 gaacaccagt agcgaaagcg gctcactggg tctcttctga cgctgatgca cgaaagctag
      241 gggagcgaag agg
//
LOCUS       KHUT01011831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011831
VERSION     KHUT01011831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agcgtgtgta ggcggtttgt
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaacgg cattcgatac tggcgagcta
      121 gagtacggta gagggtggcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccgat ggcgaaggca accacctggg cctgtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011832
VERSION     KHUT01011832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           82 g           56 t
ORIGIN      
        1 tacgtagggg gcgagagttg tccggattta ctgggtgtaa agggtgctca ggcgggtttg
       61 taagtcagag gtgaaatacc gaagcttaac ttcggcacgg cctttgatac tgcaagcctg
      121 gagtttgaaa gagggtaatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gttacctggt tcaaaactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011833
VERSION     KHUT01011833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctacg
       61 taagtcggat gtgaaatccc tcggctcaac tgaggacgtg cattcgaaac tgtgtagctt
      121 gagtatggaa gagggtcgca gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011834
VERSION     KHUT01011834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           56 c           75 g           48 t
ORIGIN      
        1 tacagagacc ccaagcatta tccggaatta ctgggcgtaa agcgcgagca tccggtcgta
       61 ttagtctggt gtaaaatcat ggggcccaac ctcatgcctg cattggaaac ggtacgactc
      121 gagcagatga gagggaggca gaacctatgg tgtaggggtg aaatccactg atatcatagg
      181 gaataccaaa agcgaaggca gcctcctagc gtcttgctga cggtcaatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01011835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011835
VERSION     KHUT01011835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          107 g           39 t
ORIGIN      
        1 gacggagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcgggcgtg
       61 tacggcggtg gtgaaagctc ctggctcaac tgggagaggc cctccgcaac ggcacgtctc
      121 gagggtggta gaggcagatg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacacccgt ggcgaaggcg gtctgctggg ccatgcctga cgctgaagcg cgaaggccag
      241 gggagtgaac ggg
//
LOCUS       KHUT01011836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011836
VERSION     KHUT01011836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           85 g           48 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggc caacagaaac taccaggctt
      121 gagtatggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacacctat ggcgaaagca ggtacctaga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011837
VERSION     KHUT01011837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          100 g           41 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcaccg
       61 taagtacgca gtaaaatctc cctgctcaac ggggagggcg ctgagtagac tatggagcta
      121 gaggtgggta gagggtggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccacctggg cccaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011838
VERSION     KHUT01011838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           61 c          105 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 cgcgtctggg gtgaaatcct ccggctcaac cggggaaggg cctcgggtac ggacggcctc
      121 gaggggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccctcactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011839
VERSION     KHUT01011839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           98 g           41 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ctcggagtta ctgggcataa agggcgcgta ggcggtcgtg
       61 cacgtcggtg gtgaaatctc cgggctcaac ccggaaactg ccttcgatac ggcgcgacta
      121 gagtgcggga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg actctctggt ccgcaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011840
VERSION     KHUT01011840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatca ttgggcgtac agggcgcgta gacggtgtat
       61 taagtcaact gttaaattcc ccggcctaac cgggggccag cagtagatac tgatgcgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011841
VERSION     KHUT01011841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          103 g           39 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatca ctgggcgtaa agggcgtcta ggcggcctct
       61 taggtcccgt gtgaaatctc ccggctcaac tgggagggtg cacgggaaac cgtgaggcta
      121 gagactcgga ggggggtgcg gaattcccgg tggagcggtg aaatgcgttg atatcgggag
      181 gaacacccga ggcgaaggcg gcaccctggt cgaggtctga cgctgaagcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011842
VERSION     KHUT01011842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcgga
       61 taagtctgat gtgaaatctc cgagcttaac tcggaaatgg cattggatac tattcggcta
      121 gagagttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caacttctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011843
VERSION     KHUT01011843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttata
       61 taagtgggat gtgaaacccc tgggcttaac ctaggacttg cataccagac tgtgtgacta
      121 gagtatggta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gccacctgga ccaatactga cgctgaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011844
VERSION     KHUT01011844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           43 a           63 c          112 g           35 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggaatta ctgggcgtga agcgcgcgca ggcggcctca
       61 tgcgcccggc gtgaaagccc ccggctcaac cggggagcgt cgtcggggac ggtggggctt
      121 gagggcgaga ggggccggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccggctggc tcgactctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011845
VERSION     KHUT01011845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           88 g           57 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcggggata
       61 taagtcagat gtgaaattcc taggcttaac ttagggactg catctgaaac tatatttctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011846
VERSION     KHUT01011846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           63 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcacg
       61 ccagtcggtg gtgaaatccc ccagctcaac tggggaacgg cctccgatac tgcgtgcctc
      121 gagggaggta ggggcaggcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011847
VERSION     KHUT01011847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           94 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcgggctct
       61 taagttgtgg gtgaaatccc gaggctcaac ctcggaactg cctgcaaaac tgggtgcctt
      121 gaggtatgga gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gcttcctggt catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011848
VERSION     KHUT01011848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccctt
       61 cgcgtcggat gtgaaagctc agagctcaac tctgagaggc cattcgatac ggaagggcta
      121 gaggcaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01011849             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011849
VERSION     KHUT01011849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           83 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcgggtgtc
       61 taagtgtaat gcgaaatcct gtggctcaac cacatggact gtattgcata ctggatgcct
      121 cgaggatatg agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gatatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg catatttctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01011850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011850
VERSION     KHUT01011850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggatga
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaaaac tgttgttcta
      121 gagtcagaca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgacctggg tctagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011851
VERSION     KHUT01011851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtcttt
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tggaaggctt
      121 gagacgatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctggc atcggtctga cgctcatgtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011852
VERSION     KHUT01011852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatta ctgggcgtaa agcgaatgta ggtggtgatg
       61 caagtcagat gtgaaagccc tgggcttaac ccgggaattg catttgatac tgcgtcgctc
      121 gagtgcagga gaggtaagtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaataccgac ggcgaaggca acttactggc ctgtaactga cactgagatt cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011853
VERSION     KHUT01011853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggct ttgaaagccc ccggctcaac cggggaattg gcgccgaaac gacccgtctg
      121 gaggggcata gggggagctg gaacttccgg tggagcggtg aaatgcgttg agatcggaag
      181 gaacgcccgt ggcgaaagcg agctcctgga tgtcttctga cgctgagacg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011854
VERSION     KHUT01011854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          105 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggctggg
       61 tatgtgactg gtgacagcct cccgctcaac gggagaaggc ccggttacac agcccggctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011855
VERSION     KHUT01011855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttgcg
       61 taagtcatct gtgaaatccc ggggctcaac tccggggccg caggtgagac tgcgtgacta
      121 gagcacagta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ctgttgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011856
VERSION     KHUT01011856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ttcggaatta ctaggtgtaa agcgcaggta gctggttgga
       61 taagtccgca atgaaatctc ccggcctaac tgggaggggt ttgtagatac tgtctgactc
      121 gagtgtggga gaggcaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaat ggcgaaagca ggttgctggc ccaatactga cggtgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011857
VERSION     KHUT01011857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 ctagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacagagac tgtcaggctg
      121 gagtatggga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaagca ggtgcctgga ccattactga cactcaactg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011858             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011858
VERSION     KHUT01011858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           62 c           99 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgc
       61 cacgtcaggg gctgaaatcc cccggctcaa ccggggaacg ggcactgata cgagcggcct
      121 ggagggaggt agggggggct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagcccctgg accttttctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011859
VERSION     KHUT01011859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c          100 g           37 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggcttgt
       61 caagtggatt gtgaaagcgc ccggcttaac cgggcgaggc cagtccagac tgataggctg
      121 gagggcagta gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgcacctga cgctgaaccg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011860
VERSION     KHUT01011860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcacgta ggcggccaga
       61 taagtcagtt gtgaaatccc tgggcttaac ctaggaacag caattgatac tgtatggcta
      121 gagtcgaaga gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacccgt ggcgaaggcg gcttcctggc ttcagactga cgctgaggtg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011861
VERSION     KHUT01011861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           57 c           96 g           36 t
ORIGIN      
        1 gacggagggc gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggacgc
       61 taagtccgac gtgaaagccc ggggctcaac cccgggactg cgctggaaac tgacgtccta
      121 gagtgcagga gaggaaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gccttctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011862
VERSION     KHUT01011862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           98 g           49 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgtc
       61 cacgtcgggt gtgaaagctc ggggctcaac cctgagaggt cattcgatac gggacggctt
      121 gaggcagcta ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg gctgttctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU11999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011863
VERSION     KHUT01011863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU11999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgctcgta ggcgggtttg
       61 taagtcgatt gtgaaagccc cgggctaaat ccgggaattg cagtcgaaac tacaagtctt
      121 gaatgctcga gagggtgacg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gttgcctggc gatgcattga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011864
VERSION     KHUT01011864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggtgtaa
       61 taagtttgat gtgaaatccc cgggcttaac ctgggaactg cgttgaagac tgttagacta
      121 gagtcgaaga gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatcagt ggcgaaggcg gcaacctggc ttcagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011865
VERSION     KHUT01011865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggcata
       61 caagtcgggc gtgaaatccc tgggctcaac ccaggaattg cgctcgaaac tgcatgtctt
      121 gagtgtcgga gaggaaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011866
VERSION     KHUT01011866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggcatat
       61 taagtcaact gttaaattcc tcggcctaac cgaggatctg cggtagatac tgatgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccacatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011867
VERSION     KHUT01011867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           81 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggaagga
       61 aaagtcagat gtgaaatcct ggggctcaac ctcagaactg catttgaaac ttttcttctc
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaagcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01011868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011868
VERSION     KHUT01011868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctcgt
       61 taagttcgat gtgaaagccc tgggcttaac ccaggaactg cattgaaaac tgatgggctt
      121 gagtccggga gagggtcgcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgacctgga ccggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011869
VERSION     KHUT01011869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ctgggcgtaa agggtgcgta gacggtttgt
       61 taagtttgtt gttaaagact ctggcctaac cggagggttg caatgaaaac tggcagacta
      121 gagggtgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011870
VERSION     KHUT01011870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          102 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 caagtcgggg gtgaaatacc ccagcttaac tggggaattg cccccgatac tggcggtctc
      121 gaggggggta ggggcacgcg gaacttccgg tggagcggtg aaatgcgttg agatcggaag
      181 gaacgccggc ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011871
VERSION     KHUT01011871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           51 c           81 g           47 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgtccttc
       61 cacgtctgat cacaaaatgc ggggctcaac cccgcatcct ggttggaaac gagaaggatc
      121 gagtcattca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaagca ggatgctggg aatgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011872
VERSION     KHUT01011872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgtcg
       61 caagtcgatg gtgaaagctc gccgctcaac ggcgagtcgg ccattgatac tgcgaggctt
      121 gagcactgta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011873
VERSION     KHUT01011873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           98 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcggct
       61 caagtcgggg gtgaaatcct tccgcttaac gggagaattg ctttcgatac tgggtcgctg
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011874             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011874
VERSION     KHUT01011874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcttt
       61 taagtctggt gtgaaaggtt cagggctcaa cccgaacagt gcactggata ctgggagact
      121 tgagtgcggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctctctgg cctgcaactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011875
VERSION     KHUT01011875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           85 g           61 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttg
       61 taagtcagtg gtgaaatatc gcagcttaac tgtgagggtg ccaatgatac tgcgaggctt
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011876
VERSION     KHUT01011876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgtt
       61 taagtccggg gtgaaagccc actgctcaac agtggaactg ccctggaaac tggacggctt
      121 gagtacagac gagggcggcg gaatggatgg tgtagcggtg aaatgcatag ataccatcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011877
VERSION     KHUT01011877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           83 g           52 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtttttcat
       61 cacgtctggc agtaaattgc gaggctcaac ctcgcaggtc tgttggaaac gagtggaata
      121 gagtcactca gaggcatctg gaatgtcatg tgtaggggta aaatccgtag atacatgatg
      181 gaacgccaaa agcgaaagca ggatgctggg ggtgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01011878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011878
VERSION     KHUT01011878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggataga
       61 caagtttgat gtgaaagccc tgggcttaac ctgggaacgg cgttgaagac tgtttatcta
      121 gagtatgaca gagggtcgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg acgacctggg tctaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011879
VERSION     KHUT01011879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c          102 g           42 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtga ctgggtgtaa agggtccgta ggcggagagg
       61 caagtctgac gtgaaagcgc ccggctcaac tgggcgagtg cgttggaaac tgcctgtctt
      121 gactcacgca gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccgactggg ggatgagtga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011880
VERSION     KHUT01011880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           80 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttctt
       61 taagtcagcg gtgaaagtcc agagcttaac tctgggattg ccattgatac tgaagaactt
      121 gagtatagtt gaggtaggcg gaattgacag tgtagcggtg aaatgcatag atattgtcaa
      181 gaacaccgat tgcgtaggca gcttactaag ctataactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011881
VERSION     KHUT01011881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggatatg
       61 taagtcagag gtgaaagccc agggcctaac cttggaactg cctttgaaac tacatatctg
      121 gagactcgga ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011882             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011882
VERSION     KHUT01011882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           90 g           55 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggattca ttgggcgtaa agggagcgta ggcggtaggg
       61 taagtcgggt gtgaaatcct ggggctcaac ctcagaactg cattcgatac tgctctgctt
      121 tgaggtatgg aggggagatt ggaattcacg gtgtagcagt gaaatgcgta gatatcgtga
      181 ggaagaccag tggcgaaggc ggatctctgg acattacctg acgctgatgc tcgaaggcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011883             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011883
VERSION     KHUT01011883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           83 g           55 t
ORIGIN      
        1 tacagaggga gctagcgttt ttcggattta ttgggcgtaa agagcgcgta ggcggtttcg
       61 caagttgata gtaaaagccc agggcttaac cctggaacgt ctatcaaaac tgcgagacta
      121 gagttcggta gaggatgatg gaattcttgg tgtaggggtg aaattcttag atatcaggag
      181 gaacaccggt ggcgaaagcg atcatctggg ccgcaactga cgctaatagc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011884
VERSION     KHUT01011884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           92 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg tccggaatca ctgggcttaa agggcgcgta ggcgggtgtt
       61 caagtccgtg gtgaaatcct ccggctcaac cggagaactg cctcggatac tgaatgtctc
      121 gagggtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccacttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011885
VERSION     KHUT01011885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           85 g           51 t
ORIGIN      
        1 tacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggatgg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaaatg cttggtatac tgccgttctt
      121 gaatcaccta ggggcagctg gaactgatgg tggagcggtg aaatgcatag atatcatcag
      181 gaacgccaat ggtgcagaca agctgctggg ggtgtattga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011886
VERSION     KHUT01011886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          100 g           44 t
ORIGIN      
        1 tacggagggg gcgagtgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcctgg
       61 caagtcgagt gtgaaatccc ttggcttaac tgaggaatgg cgctcgaaac tacctcgctg
      121 gagggcggga gagggaagtg gaactctcgg tgtaggggtg aaatctgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctggc ccgtacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011887
VERSION     KHUT01011887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           97 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcttgta ggcggtccgt
       61 taagtccggt gtgaaatctc ccggctcaac tgggaggggt catcggatac tggcggactg
      121 gaggtgggca gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctggg cctatcctga cgctgaggag cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01011888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011888
VERSION     KHUT01011888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggcccgt
       61 gaagtcagga gtgaaaactc ggggctcaac cccgagattg cttttgaaac cagcgggctt
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg gctttctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011889
VERSION     KHUT01011889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 cacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccatt
       61 taagtagggt gtgaaatccc ccggctcaac cggggaatgg cgctctaaac tggatggctg
      121 gagtgagata ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccgtt ggcgaaagcg gcacactggg tctcaactga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011890
VERSION     KHUT01011890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           95 g           52 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcctag
       61 caagttgggt gtgaaatccc atggcttaac tgtggggggt catccgatac tgctaagctt
      121 gaggtcggta gaggaaggta gaattcccgg tgtagtggtg aaatgcatag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctggg ccgatcctga cgctgaggcg cgaaagcgtg
      241 ggaagcgaac cgg
//
LOCUS       KHUT01011891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011891
VERSION     KHUT01011891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccag
       61 ccagtccggg gtgaaatctt tcggctcaac cggaaaaggg ccttggatac gactggtcta
      121 gagggagata ggggtctgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcggactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011892
VERSION     KHUT01011892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgat
       61 caagtcaggg gtgaaagcct ccagctcaac tggagaagtg cctttgatac tggtcgtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccgat ggcgaaagca gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011893
VERSION     KHUT01011893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggccgag
       61 caagtgagac gtgaaatccc cgggcttaac ctgggaactg cgtcttatac tgttaggcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011894
VERSION     KHUT01011894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaacgg
       61 tcagtctggc ctgaaagccc ccggctcaac cggggagggt ggctggaaac tgccgttctt
      121 gaggatgaga gaggcgtgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcacgctggc tcatccctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011895
VERSION     KHUT01011895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           79 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctaat
       61 taagtcagtg gtgaaatcct gcagctcaac tgtagaattg ccattgatac tgaatagctt
      121 gaatatagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaaccccgat tgcgaaggca gcttactaga ctgtaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011896
VERSION     KHUT01011896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           82 g           62 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcaat
       61 taagtcagtg ctgaaatatc ccggcttaac cgggagggtg gcattgatac tgattgactt
      121 gagtggagtt gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccgat agcgaaggca gcttaccaag ctctaactga cactgatgca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011897
VERSION     KHUT01011897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           83 g           58 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggttgttatg
       61 taagtagagt gtgaaatccc tgggctcaac cgaggaagtg cactctaaac tacatgacta
      121 gagggaagta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg accttctggg cttctcctga cactgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011898
VERSION     KHUT01011898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgtgtgca ggcggggctg
       61 taagtggtgt atgaaatctc tcggctcaac cgagataggt tatgccagac tgcagctctt
      121 gagtgcgaga gaggagcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgctctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011899
VERSION     KHUT01011899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           91 g           50 t
ORIGIN      
        1 gacgaactgc cctaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggcgaat
       61 caagtcaggg gtgaaatccg gcagcttaac tgtcgaagtg cccttgatac tgatttgctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011900
VERSION     KHUT01011900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggacgtg
       61 caagtgagat gtgaaatacc cgggctcaac ctgggaactg catcttaaac tgcaagtctg
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011901
VERSION     KHUT01011901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggttctg
       61 caagtcagat gtgaaagccc tgggcttaac ctgggaatgg catttgaaac tagggaacta
      121 gagtgtagca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ctaacactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011902
VERSION     KHUT01011902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           84 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaatctc tgagctcaac tcagaaactg cattggatac tatccggctc
      121 gaggagtgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctaga cacttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011903
VERSION     KHUT01011903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           80 g           59 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ctcggattta ttgggcgtaa agggtgtgta ggtggtttgg
       61 taagttagat gtgaaagccc agggcttaac cctggaaccg catttaagac tgccttactt
      121 gaagatcaaa gaggcaagca gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaataccaat ggcgtaggca gcttgctggt tgatacttga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011904
VERSION     KHUT01011904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtctgat gtaaaagact ggggctcaac ctcgggactg cattggatac tggcagactg
      121 gagtacggta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaagcg acttgctggg ccgtaactga cgctgaggaa cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01011905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011905
VERSION     KHUT01011905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           54 c          101 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggccctg
       61 tttgtccggc gtgaaatccc agggctcaac cttggggctg cgctggatac ggcggggctt
      121 gaggcgagga gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggc cttgatctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011906
VERSION     KHUT01011906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           85 g           57 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggcggctttc
       61 taagtcggtt gtgaaatccc atggcttaac catggaattg cattcgaaac tggagagctt
      121 gagtgtggga gagattggta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccggt ggcgaaggcg gccaattggc ccaacactga cgctgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011907
VERSION     KHUT01011907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacagaggcc tcgagcgttg cgcggaatca ctgggcttaa agggtgcgta ggtggacatg
       61 caagtgcctt gtgaaatccc cgggctcaac ccgggaattg cttggtagac tgcatgtctt
      121 gaggtcggta ggggtcgctg gaacagttgg tggagcggtg aaatgcgtag atatcaactg
      181 gaacgccaaa ggcgaaggca agcgactggg ccgattctga cactgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011908
VERSION     KHUT01011908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcgggcttc
       61 taagttggag gtgaaatgcc gaggctcaac ctcggaactg cctccaaaac tgggagcctt
      121 gaggtatgga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011909
VERSION     KHUT01011909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggacaat
       61 caagtcaggt gtgaaatccc cgggctcaac ctgggaactg catctgaaac tggttgtctt
      121 gagtgtcgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cgacaactga cgctgagacg cgaaagtgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011910
VERSION     KHUT01011910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggttcgta ggtggtcgaa
       61 taagtctgac gtgaaatccc atggctcaac cgtggaactg cgttggaaac tgtacgactt
      121 gagtgccgca gaggtaaagg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg ctttactggg cggttactga cactgaggaa cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01011911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011911
VERSION     KHUT01011911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          104 g           43 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtccat
       61 cgcgtccggt gtgaaatctc ccggctcacc tgggaggggg cgctggaaac ggatggactg
      121 gaggcaggca gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gccgactggg cctgttctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011912
VERSION     KHUT01011912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           73 g           64 t
ORIGIN      
        1 gacgaaggac ccaagcgtta tccggattca ttgggtttaa agggtgcgta ggtggattga
       61 taagtcagtg gtgaaatcct gcagcttaac tgcagaactg ccattgatac tgttgatctt
      121 gaatatgttt gatgtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaag ccattattga cactgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011913
VERSION     KHUT01011913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           78 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcagt
       61 taagtcagtg gtgaaatccc ggagctcaac tccggaactg ccattgatac tatctgtctt
      121 gaatattgtg gaggtttgcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcaggctaca catatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011914
VERSION     KHUT01011914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           91 g           41 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggtcagc
       61 taagacagag gtgaaatccc cgggctcaac ctgggaacgg cctatgtgac tggcaggcta
      121 gagtatggca gagggaggtg gaattccatg tgtagcagtg aaatgcgtag agatatggag
      181 gaacaccgat ggcgaaggca acctcctggg ccaatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011915
VERSION     KHUT01011915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           87 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgtagta
       61 taagttgtct gtgaaattcc tgggcttaac ctaggcatgt cagatgatac tgtattacta
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011916
VERSION     KHUT01011916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccagg
       61 taggtcggct gtgaaaactc gaggctcaac ctcgagctgt cggccgaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011917
VERSION     KHUT01011917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           82 g           57 t
ORIGIN      
        1 tacgagtgct ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtctttt gttaaattct tcggcttaac cgggggcatg caaaggaaac ggcacaacta
      121 gaggatgcga gaggtgaacg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca gttcactggc gcactcctga cactgagaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01011918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011918
VERSION     KHUT01011918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccga
       61 aaagtctggt gtgaaatcct ggggctcaac cccagactgt cactggatac tgtcgggctt
      121 gaatccggaa gaggagaatg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gttctctggt acgtgattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011919
VERSION     KHUT01011919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtggac
       61 taagtcgggt gtgaaatctc cgggctcaac ccggagcatg cgtccgaaac tgattcacta
      121 gaggatggga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga ccattcctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011920
VERSION     KHUT01011920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggttgaa
       61 taagttcttt gtgaaagctc ctggcttaac tgggagaggt caaggaatac tgttcggcta
      121 gagtacggta ggggaaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca agtttctggg ccgttactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011921
VERSION     KHUT01011921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           84 g           56 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatata
       61 taagtcagaa gtgaaatccc ggggcttaac cccggaactg cttttgagac tgtatgtctg
      121 gaatccggta gaggttgatg gaatatccag tgtagaggtg aaattcatag atattggata
      181 gaacaccagt ggcgaaggcg atcaactggg ccggtattga cgctgaggta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011922
VERSION     KHUT01011922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggccttg
       61 taagtcggat gtgaaatccc acagctcaac tgtggacgtg cattcgaaac tgctcggctt
      121 gagtatggaa gagggtcgca gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccggt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011923
VERSION     KHUT01011923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcggcctgg
       61 taagtcgaat gtgaaagccc atggcttaac catggaagtg cattcgaaac tgcctagctc
      121 gagtatcgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccagt ggcgaaggcg accgcctgga cgattactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011924
VERSION     KHUT01011924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           98 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgagta ggcggcttgt
       61 taagtcgcgt gtgaaagctc ccggctcaac cgggaaagtg cgcgcgaaac tggcgagctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga aggacactga cgctgagaag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011925
VERSION     KHUT01011925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          104 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgccta ggcggcggtt
       61 taagtctgcg gtgaaaggcc gaggctcaac ctcggacggt ccgcggagac tggatcgctg
      121 gagtatcgga gaggagcggg gaatccctgg tgtagcggtg gaatgcgtag agatcagggg
      181 gaacaccgat ggcgaaggca ccgctctggc cgactactga cgctgaagcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011926
VERSION     KHUT01011926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           98 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatta ctgggcgtaa agagcgtgta ggcggcatga
       61 gaagtctgct gtgaaagctc tcggcttaac cgggagaggt cggcagaaac tctcaagcta
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01011927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011927
VERSION     KHUT01011927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 tacagaggcc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggcccg
       61 caagtgcttt gtgaaatccc acggctcaac cgtggaatgg ctgggcagac tgcgggtctt
      121 gaggttggta ggggtcgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccaattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011928
VERSION     KHUT01011928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          100 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca gacggatgac
       61 tacgtgcttt gtgacatctc caagcttaac tgggaggagc caaggtacac gggtcgtcta
      121 gagtggtcga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcatcctggc gacatactga cgttgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01011929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011929
VERSION     KHUT01011929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtttct
       61 caagtcagag gtgaaagccc agggctcaac cctggaatag cctttgagac tgggagactt
      121 gaacacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgttgttga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011930
VERSION     KHUT01011930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcctgg
       61 aaagtctggt gtgaaatctt agggctcaac cctaagctgt cactggatac tgtcaggctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011931
VERSION     KHUT01011931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgat
       61 caagtccggg gtgaaatcct tcagctcaac tggagaatag ccttggatac tggtcgcctc
      121 gagggaggta ggggcgggcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011932
VERSION     KHUT01011932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           95 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgcgg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tgccgtgctg
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gcttactgga ccactactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011933
VERSION     KHUT01011933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctaag
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tacctagctt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011934
VERSION     KHUT01011934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           89 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgta ggcggacgga
       61 taagttattg gtaaaaagct caggcttaac ctgagacagg ctaataagac tgtcggtctt
      121 gagtcgaata gaggggggta gaatttccgg aggagcggtg aaatgcgtag atatcggaaa
      181 gaagaccggt ggcgaaggcg gccccctggg ttcaaactga cgctaaagca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011935
VERSION     KHUT01011935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           84 g           58 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggcatta ttgggcgtaa agggtgtgta gacggcttat
       61 taagtcaact gttaaattct ccggcctaac cggaggtctg cggtagatac tgattggcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011936
VERSION     KHUT01011936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           86 g           47 t
ORIGIN      
        1 tacagagact gcgagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggctttg
       61 tgtgtgaggc gtgaaagccc ggggcttaac cccggaattg cacctcaaac tacaaggcta
      121 gagcattgga gagggtagca gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaataccaga ggcgaaggcg gctacctgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011937
VERSION     KHUT01011937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggcctgg
       61 taagtctgct gtgaaaacct ggggctcaac cccgggcgtg cagtggatac tgccaggcta
      121 gaggacggta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccgttcctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011938
VERSION     KHUT01011938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagagact gcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcggt
       61 taagtcggat gtgaaatctc acagcctaac tgtgataggt cattcgaaac tagccggctg
      121 gaggactgga gaggagactg gaatggtcgg tgtagcggtg aaatgcgtag agatcgacca
      181 gaacaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011939
VERSION     KHUT01011939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggccgcc
       61 tgtgtcgagt gtgaaatccc aaggctcaac cttggaatgg cgctcgaaac tgggtggctt
      121 gagtgcagga ggggagagcg gaattcttgg tgtagcggtg aaatgcgttg atatcaagaa
      181 gaacaccggt ggcgaaggcg gctctctgga atgcaactga cactgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011940
VERSION     KHUT01011940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           87 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgata
       61 taagttaggt gtgaaatact tgggcttaac ctgggtactg cacttaagac tgtatagcta
      121 gagtagagta gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gctttctgga cttatactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011941
VERSION     KHUT01011941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           95 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgta ggcggttggc
       61 taagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tggctgactt
      121 gaggcgatga gagggacgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcgtcctagc atcggcctga cgttcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011942
VERSION     KHUT01011942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 gacgaaggtg gcaaccgttg ctcggattca ctgggcataa agcgcgcgta ggtggtgacg
       61 caagtcactg gtgaaatccc ccggcccaac cggggaattg ccggtgaaac tgcgttgctt
      121 gaggacggga ggggagagtg gaactcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg gctctctgga ccgtacctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011943
VERSION     KHUT01011943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggacttt
       61 taagccagac gtgaaagccc cgggcttaac ctgggaatgg cgtttgggac tgggagtctg
      121 gagtacggca gaggagactg gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011944
VERSION     KHUT01011944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           83 g           56 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggcaatt
       61 aaagttgctg gtaaaatggc caagcttaac ttggtgttgg ctagcaatac ttaaatgctg
      121 gggaaccgaa tgggaaactg gaattctcga tgtagcagtg aaatgcgtag atatcgagag
      181 gaacatcagt ggcgaaagcg agtttctggg cggattccga cgctgaggca cgaaagcgaa
      241 ggtagcaaac ggg
//
LOCUS       KHUT01011945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011945
VERSION     KHUT01011945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggtttta
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaattg cgttgaatac tgtaggacta
      121 gagtctggca gagggtcgtg gaatttccgg tgtagcagtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgatctggg tcaagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011946
VERSION     KHUT01011946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 cacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccccg
       61 taagtggtgt gtgaaatgcc ctggctcaac cagggaatag cgccccaaac tgcggggatt
      121 gaggacagta gaggaagctg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccgtt ggcgaaggcg agcttctggg ctgttcctga cgctgatgcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011947
VERSION     KHUT01011947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           92 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccac
       61 caagttggat gtgaaagccc tcggcttaac tgaggaagtg catacaaaac tagtggactt
      121 gagtacggga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacgccagt ggcgaaggcg gcgatctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcagac agg
//
LOCUS       KHUT01011948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011948
VERSION     KHUT01011948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          101 g           43 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgcga
       61 gaagtcaggc gtgaaagccc cgggctcaac ctgggaatgg cgcttgggac tctcgtgctc
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011949
VERSION     KHUT01011949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c          100 g           41 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcccgt
       61 ttagtcggtt gtgaaaaccc ggggctcaac cccgggcccg cgtccgatac tggcgggcta
      121 gaggcaggca ggggagagcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01011950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011950
VERSION     KHUT01011950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           83 g           58 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtgtgat gttaaatcgt ggggctcaac cctatcgatg cattgcatac tggcagacta
      121 gagggaagca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg cttcttctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011951
VERSION     KHUT01011951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agggcgcgta ggcggatttt
       61 taagtctggt gtgaaaactt ggggctcaac cccaagcctg cattggaaac tggagatctt
      121 gaggacagga gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011952
VERSION     KHUT01011952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta gtctgttata
       61 caagtcaggg gtgaaatgcc acagctcaac tgtggaactg cctttgaaac tgtatggcta
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgattaggcg cgaaagcacg
      241 gggatcaaac agg
//
LOCUS       KHUT01011953             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011953
VERSION     KHUT01011953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           51 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggttcag
       61 taagtcggat gtgaaaactc ggggctcaac cccgagacgc catccgatac tgctgtgact
      121 tgagtctggt aggggagatc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggatctctgg gccagcactg acactgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011954             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011954
VERSION     KHUT01011954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatta ctgggcttaa agcgcgtgta ggcggaccgg
       61 cacgtccgtt gctgaaatcc cccggcttaa ccggggaagt ggcacagata cggccggtct
      121 ggagggaggt aggggggtct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg accttttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011955             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011955
VERSION     KHUT01011955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           75 g           60 t
ORIGIN      
        1 tacagagggt gcaagcgtta tccggaatta ttgggcgtaa agcgtgtgta ggcggagtgg
       61 taagtcgagg attaaatttc caagcttaac ttggagcctg ttctcgaaac taccattctt
      121 tgaatcatgc ataggcaaat ggaattctgt gtgtaggggt aaaatccgta gatacacgga
      181 ggaacaccaa aagcgaaagc agtttgctgg gcatgtattg acgctgagac acgaaagcgt
      241 ggggatcaaa cagg
//
LOCUS       KHUT01011956             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011956
VERSION     KHUT01011956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           87 g           52 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaacc gctttacata ctggcgagct
      121 tgaggaatgc agaggttagt ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc agctaactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011957             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011957
VERSION     KHUT01011957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgact gtgaaaacct gcggctcaac cgtgtggtct gcagtcgata cgggcaggct
      121 agaattcggt aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc aggtctctgg gccgatattg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011958
VERSION     KHUT01011958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          104 g           38 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggtggtccat
       61 gatgtgccgt gtgacatctg gaggcttaac ctccagcggc cagggtagac aggtggactg
      121 gaggcaggga gagggctgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcagcctggc cctgtactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011959
VERSION     KHUT01011959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccagc
       61 cgcgtcggat gtgaaagctc agagctcaac tctgagatgc cattcgatac gggccggcta
      121 gaggcaggta gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01011960             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011960
VERSION     KHUT01011960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           95 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctttt
       61 taagtctggt gtgaaaggtt cagggctcaa cccgaacagt gcactggata ctggagagct
      121 tgagttaggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctctctgg cctataactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011961
VERSION     KHUT01011961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           99 g           39 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattaa ctgggcgtaa agcgcacgca ggtggtcgcg
       61 ggcgtcggta ctgaaagttc gccgcttaac ggcgaaaggc gtaccgatac gccgcgactg
      121 gaggtcgtga gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccaa tgggaagcca gcggactggc acggtgctga cactgaggtg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011962
VERSION     KHUT01011962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          100 g           45 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggagtga ctgggtgtaa agggtccgta ggcggggact
       61 caagtctgac gtgaaagcgc ccggctcaac tgggcgagtg cgttggaaac tgggtctctt
      121 gaatcacgca gaggtcggcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccgactggg tgatgattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01011963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011963
VERSION     KHUT01011963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggcctgt
       61 caagttggct gtgaaagctc ccggcttaac tgggagaggt cagtcaaaac tgacaggcta
      121 gaggctgaga taggcgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctcgctggt tcagccctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011964
VERSION     KHUT01011964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgctgga gaggctacta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccgga ggcgaaggcg ggtagccagc cagacactga cactcagacc cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01011965             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011965
VERSION     KHUT01011965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           81 g           52 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggcggcatt
       61 gtaagcaggt agtgaaagcg tgtggctcaa ccatactcac attacctgaa ctgcaaagct
      121 agaggacaag agaggtcact ggaattccta gtgtaggagt gaaatccgta gatattagga
      181 ggaacaccga tggcgtaggc aggtgactgg cttgttcctg acgctcaggc acgaaagcgt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01011966             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011966
VERSION     KHUT01011966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           52 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattta ctgggcgtaa agagcacgta ggcggtctgt
       61 taagtgagaa gtgaaatctc cagcgctcaa cgcggaaacg gcttttcata ctggcagact
      121 tgagggatgc agaggcaagt agaattcctg gtgtaccggt gacatgggtt gatatcagga
      181 ggaataccaa tggcgaaggc agcttgctgg gcatcatctg acgctgaggt gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01011967             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011967
VERSION     KHUT01011967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctact
       61 gaaagtttga tgtgaaatct ccgggcttaa cccggaaaat gcattgaata ctgcagagct
      121 ggagggttgg aggggggact ggaattctcg gtgtagcagt gaaatgcgta gatatcgaga
      181 ggaacaccag tggcgaaggc gagtccctgg acaacacctg acactgaggc acgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011968             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011968
VERSION     KHUT01011968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           95 g           51 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggtccgta ggcggttgca
       61 taagtctggc gtgaaatctt caccgctcaa cgggaagagg ccgctggaaa ctgcgcaact
      121 tgagtatggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatctg tggcgaaggc ggcttgctgg gccattactg acgctgaggg acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011969
VERSION     KHUT01011969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           35 c           71 g           70 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtagag
       61 taagtcagtg gtgaaagttt gcagcttaac tgtaaaattg ccattgatac tgctttactt
      121 gaattactta gaagttacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggtaactatg agttaattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01011970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011970
VERSION     KHUT01011970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agggtccgtc ggtgggcgcg
       61 caagtctggt gtaacagccc ctggcttaac tgggggtagc cactggaaac tacgcgtctt
      121 gagtcacgga gaggggtgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccaaa ggcgcaagca gcaccctggt cgaggactga cacttcagga cgaaagtatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011971
VERSION     KHUT01011971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ttgggcgtaa agcgtgcgca ggtggtgcag
       61 gaagtgatgt gtgaaagctc ttggcttaac tgagagaggc catgtcaaac tcctggactt
      121 gagggtcgga gaagcagacg gaattccggg tggagtagta atatgcgtag agatccggag
      181 gaacaccagt ggcgtaagcg gtctgctaga cgatgcctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01011972             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011972
VERSION     KHUT01011972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           90 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agggctttta ggcggcttag
       61 caagtcgggt gtgaaacctc caggctcaac ctggagtcgc cattcgatac tgctatggct
      121 agagtctggt aggggatcac ggaattcctg gtgtagcggt gaaatgcata gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccagtactg acgctgaaga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01011973             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011973
VERSION     KHUT01011973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           93 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggtggatcta
       61 tgcgtcaggt gtgaaagccc tgggctcaac ccaggaatgg catctgatac ggtagatcta
      121 agagtacgag aagggaaagc ggaatggcga gtggagcagt gaaatgcgtt gagattcgtt
      181 ggaacaccgg aggcgaaggc ggctttctgg ctctgtactg acactaaggc gcgaaagtgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011974
VERSION     KHUT01011974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggctaga
       61 taggtccgtt gtgaaaactg gaggctcaac cttcagacgt cgacggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011975
VERSION     KHUT01011975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           95 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttcgt
       61 taagtccgat gtgaaagccc tgggctcaac ctgggaatgg catgggatac tggcgggcta
      121 gagtgcggta gagggcagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg actgcctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011976             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011976
VERSION     KHUT01011976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           76 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcata
       61 gtaagtagat agtgaaatcg tgtggctcaa ccatacatcc attatctaaa ctgctaagct
      121 agaagatgag agaggttatt ggaattccca gtgtaggagt gaaatccgta gatattggga
      181 ggaacaccga tggcgtaggc agataactgg ctcattcttg acactaaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011977
VERSION     KHUT01011977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatttt
       61 taagtcgggg gtgaaatccc ggagctcaac tccggaactg cctccgatac tgagaatctc
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011978
VERSION     KHUT01011978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           65 c           98 g           37 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccgc
       61 aaagtcgggg gtgaaatccc acggcccaac cgtggaagtg cctccgatac tggcgggctc
      121 gagcgaggta ggggcgggcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011979
VERSION     KHUT01011979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          105 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggtcgag
       61 caggtacctg gtgacatcct cgcgctcaac gcgaggcggt ctgggtaaac cgcacgactg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011980
VERSION     KHUT01011980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaaagt
       61 taagcgaggt gtgaaagccc ccggctcaac cggggaaggt cgcttcgaac tggctttctt
      121 gaggacgtga gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggt gcgtacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01011981             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011981
VERSION     KHUT01011981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           84 g           52 t
ORIGIN      
        1 tacgagagcc tcaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggtcgtg
       61 ttagtcctgt gtgaaagcct gtcgcttaac gacagatccg catgggaaac ggcacgactg
      121 cgagggtgta agaggtgaat ggaactcacg gtgtaggggt gaaatccgtt gatatcgtgg
      181 ggaacgccaa aagcgaaggc aattcactgg tacatacctg acactgaaac acgaaagcca
      241 gggtagcgaa tggg
//
LOCUS       KHUT01011982             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011982
VERSION     KHUT01011982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggcacta
       61 tcagtcgggg gtgaaatccc atcggcccaa ccgtggaatt gccttcgata ctgtagtgct
      121 tgaggaaggt aggggtccgt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccgg tggcgaaagc gacggactgg atcttttctg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011983             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011983
VERSION     KHUT01011983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggctcag
       61 caagtcggcc gtgaaaaccc ggggctcaac cccgggacgc cggtcgatac tgctgtggct
      121 agggttcggt agaggaacgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcgttctgg gccgacaccg acgctgagga gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01011984             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011984
VERSION     KHUT01011984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           94 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcctt
       61 taagttaatt gtgaaatctc cacggctcaa ccgggagact gcggttaaga ctgggggact
      121 agagtatggg agagggtagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccaa tggcgaaggc agctacctgg cccatttctg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011985             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011985
VERSION     KHUT01011985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c          101 g           42 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcggatcgg
       61 cgggtctggc gtgaaatctc cactgctcaa cagggagagg ccgctggaaa ctaccgatct
      121 tgagtgtggt agaggaccgt ggaattccgc gtgtagcggt ggaatgcgta gatatgcgga
      181 agaacaccgg tggcgaaggc ggcggtctgg gccacaactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011986             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011986
VERSION     KHUT01011986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           82 g           53 t
ORIGIN      
        1 tacgtaaggt ccaagcgtta tccggaatta ctgggcgtaa agcgtctgta ggcggcgcag
       61 aaagttaggt gcgaaatctt gcggctcaac cgtatagact gtgcttaaaa cttctgtgct
      121 tgaggttggt agaggcaagt ggaacttgcg gtgtaacagt gacatgtgta gatatcgcaa
      181 agaacaccaa tggcgaaggc agcttgctgg gccacacctg acgctcagag acgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01011987             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011987
VERSION     KHUT01011987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           59 c           97 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggggcgg
       61 aacgtcggta actgaaagcc cccggctcaa ccggggaagg ggtgccgaaa cgaccatcct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctactg acgctgagac gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01011988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011988
VERSION     KHUT01011988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           92 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagcttgta ggcggtttgt
       61 cgcatctgct gtgaaatctc aaggctcaac cttgagcttg cagtgggtac gggcagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ccactactga cgctgagaag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01011989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011989
VERSION     KHUT01011989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           87 g           57 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agagtatgta ggcggccttg
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg catttgatac tgcttggctt
      121 gaatgttgga gaggatagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctatctggc caaacattga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01011990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011990
VERSION     KHUT01011990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 gacggaggag gctagcgttg ttcggaatta ctgggcgtaa agcgtacgca ggtggcgaag
       61 caagtcggat gtgaaagccc ggggctcaac cccggatgtg catccgatac tgttttgctg
      121 gagtccggaa gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctggt ccggtactga cactcaagta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011991
VERSION     KHUT01011991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccttt
       61 taagtctgct gtgaaagtcc agggctcaac cctgggatgt cagtggatac tggaaggctc
      121 gagtccggaa gaggcaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gcttgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011992
VERSION     KHUT01011992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           97 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggcgaga
       61 cgagtgggac gtgaaagccc cgggcttaac ctgggaagtg cgtaccaaac tgtcttgctg
      121 gagtatagga gagggcggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgtcaat ggcgaaggca gccgcctggc ctaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011993
VERSION     KHUT01011993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggtacgg
       61 caagtctgct gtgaaagcgc ccggctcaac tgggcgaggt cggtggaaac tgctgaacta
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg ccgctcctga cactgagacg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011994
VERSION     KHUT01011994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggattta ctgggcgtaa agcgcgcgta ggcggctaat
       61 taagtcaaat gtgaaatccc cgagcttaac ttgggaattg cattcgatac tggttagcta
      121 gagtgtggga gaggatggta gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaataccgat ggcgaaggca gccatctggc ctaacactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01011995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011995
VERSION     KHUT01011995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          101 g           38 t
ORIGIN      
        1 tacagaggtg tcaagcgttg ctcggtgtca ctgggcttaa agggcgcgta ggctgtgcag
       61 tacggctcgg gtgaaatccc tcggcccaac cgaggaactg cccgggcgac ggctgcgctg
      121 gactgcatca ggggcggatg gaactcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg atccgctggg gtgcaagtga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011996
VERSION     KHUT01011996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           92 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggatctt
       61 taggtcagag gtgaaatctt ggggctcaac cccaaaactg cctttgaaac cggaggactt
      121 gaggtatgga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01011997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011997
VERSION     KHUT01011997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           92 g           46 t
ORIGIN      
        1 gacggaggag gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggctcga
       61 ttagtcggat gtgaaatccc ggggctcaac cccggaactg cctccgatac tgtcgagctt
      121 gagcggagga gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctggc ctcttgctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01011998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011998
VERSION     KHUT01011998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcttg
       61 taagtcgaat gtgaaagccc ccggctcaac cggggagggt cattcgatac tgcttgactc
      121 gaaggcagga gagggaagcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gctttctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01011999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01011999
VERSION     KHUT01011999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           92 g           50 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggccatg
       61 caagtacttt gtgaaatccc tcggctcaac cggggaattg cttggtatac tgcagggctt
      121 gaggcgggta ggggtgcttg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcctgt ggcgaaagcg gagcactggg cccgtcctga cgctaagacg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012000
VERSION     KHUT01012000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          104 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgtta tccggaatca ctgggcgtaa agcgctggta ggcggtttgg
       61 tgggtgcgtg gtgaaagccc ctggcttacc cagggagggt ccgcgcctac cgccagactc
      121 gaggcaagca gaggactgtg gaacgtccgg gggagtggtg gaatgcgtag atctcggacg
      181 gaacaccgga ggcgaaggcg gcagtctggg cttgtcctga cgctgagagg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012001
VERSION     KHUT01012001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           81 g           58 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaataa ttgggcgtac agggtgtgta gacggcatat
       61 taagtcaatt gtttaattct ccagcctaac tggaggtcag cgatagaaac tggtgtgctt
      121 gagtatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaagca gcttcttggt ccattactga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012002
VERSION     KHUT01012002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 gacgaaccgt acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggcgcag
       61 aaagttggat gtgaaagccc ccggctcaac cggggaactg cgtccaaaac tactgtgctc
      121 gagggagaca ggggtgagcg gaactcacgg tggagcggtg aaatgcgttg atatcgtgag
      181 gaacgccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012003
VERSION     KHUT01012003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           93 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctcgt
       61 caagttgggt gtgaaagccc tcggctcaac cgaggaagtg cactcaaaac tggcgagctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012004
VERSION     KHUT01012004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttaaa
       61 cgggtcggat gtgaaagccc ttggcttaac caaggaagtg cgtccgaaac tatttagcta
      121 gagtacggga gaggagggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaagcg actctctgga ccgtaactga cgctaagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012005
VERSION     KHUT01012005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggatttg
       61 caagtttgat gtgaaagccc cgggcttaac ctgggaattg cgttggaaac tgcaagacta
      121 gagtcggaca gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gctgcctggg tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012006
VERSION     KHUT01012006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgtcg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtccag
       61 caagtcggat gtgaaatccc taggcttaac ctaggaactg cattcgaaac tactagactc
      121 gagtgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012007
VERSION     KHUT01012007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           96 g           39 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtaa agcgcacgta ggtggtacgg
       61 caagtggtgt gtgacagcag ccggcttaac cggttgacgc cacgccaaac tggcgtacta
      121 gagggatgca gaggttggtg gaattgccgg tgaagcagtg aaatgcgtag ataccggcag
      181 gaacaccgca ggggaagcca gccaactggg catcacctga cactgaggtg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01012008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012008
VERSION     KHUT01012008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           82 g           55 t
ORIGIN      
        1 tacggagggt ccaaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggatttg
       61 taagtgtacg attaaagacc gacgcctaac gtcgggagtg ttgtacaaac tgcatatctt
      121 gaatttatca ggggcaagcg gaactttggg tgtaggggtg aaatccgtag atatccaaag
      181 gaacaccaat ggcgaaggca gcttgctggg gtaatattga cactgagatg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012009
VERSION     KHUT01012009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcgggcttg
       61 taagtcgatt gtgaaatccc tgggctcaac ctaggaattg cagtcgaaac tgcaagtctt
      121 gaatgcttga gaggatggcg gaattcctgg tgtagtagtg aaatacgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gctatctggc aatgcattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012010
VERSION     KHUT01012010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 gacgagggac cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgccgga gaggttactg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg ggtaactggc cggacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012011
VERSION     KHUT01012011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ctcggaatca ctgggcgtaa agcgcacgta ggcggatttg
       61 tatgtcaatt gtgaaagccc caggctcaac ctgggaatcg cagttgaaac tgcatttctt
      121 gaatacttga gagggtgggg gaatacctag tggagaagtg aaattcgtag agattaggtg
      181 gaacaccaga ggcgaaggcg cctgcctggc aatgtattga cgctgaggtg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01012012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012012
VERSION     KHUT01012012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           95 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgagta ggcggatgga
       61 taagtgaggt gataaagaca tgggcttaac ctgtgggcga cgccttagac tgtttatcta
      121 gagacgagga gaggggagtg gaatttccgg aggagtggtg aaatacgtag atttcggaag
      181 gaacaccggt ggcgaaggcg actttctggc ctcgttctga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012013
VERSION     KHUT01012013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           92 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcgcgta ggcggcgccg
       61 ttagtcagag gtgaaatacc acggctcaac cgtggaattg cccctgatac tgcggcgctc
      121 gagggaggta ggggtctgtg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccagt ggcgaaagcg acagactgga tcttttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012014
VERSION     KHUT01012014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcacct
       61 taagtcagat gtgaaaaccc agggctcaac tttgggactg catttgaaac tgaggagcta
      121 gagtgccgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012015
VERSION     KHUT01012015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          100 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtccggg gtgaaagcgc ccggcttaac cgggcaagtg cctcggatac tgctggcctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctggc cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012016
VERSION     KHUT01012016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           84 g           57 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctttg
       61 taagtcagga gtgaaatccc ggagcttaac tccggaattg cttttgaaac tacaaggcta
      121 gagtatgtta gaggatatgg gaattcctag tgtagaagtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg catatctggg acatcactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012017
VERSION     KHUT01012017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           96 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggttgtcgtt
       61 taagtcgggt gtgaaagacc ggggctcaac cccggaactg cattcgatac tgggggactg
      121 gagggttcga ggggagtctg gaattcacgg tgtagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagt ggcgaaggcg ggactctgga gaacacctga cactgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012018
VERSION     KHUT01012018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           81 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca gacggtatgt
       61 taagtcaact gttaaatttc ccagcctaac tgggagcaag cagtagaaac tggcgtacta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012019
VERSION     KHUT01012019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggttcgc
       61 caagtctggg gtgaaatctt tcggctcaac cggaaaattg ccttggatac tggcggactc
      121 gaaggaggta ggggcagtgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cactgctgga tctcccttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012020
VERSION     KHUT01012020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          105 g           36 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggcctcc
       61 tcagtcggac gtgaaagccc ggggctcaac cccggaacgg cgtccgagac gggggggctg
      121 gagaccggga gaggagggtg gaatagccag tgtagaggtg aaattcgtag atattggctg
      181 gaacaccggt ggcgaaagcg gctctctggc ccgggtctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012021
VERSION     KHUT01012021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcataa agcgcatgta ggtggtttag
       61 taagtctgat gtgaaagccc cggactcaat ccgggaagtg cattggatac tactagacta
      121 gagtatggga gaggtgagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctcactgga ccattactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012022
VERSION     KHUT01012022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c          100 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcgtgta ggcggtctgt
       61 taagtcggga gtgaaatgcc agggctcaac cctggaacgg ctttcgatac tggcggactt
      121 gagtgctcta gagggcagtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actgcctggg gagtaactga cgctgaggcg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012023
VERSION     KHUT01012023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          103 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggatggg
       61 aaagcctggg gtgaaaggtt ggagctcaac tccaacagtg ccctggggac ttcttgtctg
      121 gaggcaggca ggggatcgtg gaattcctgg tgtagcggtg gaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcgatctggg cctgtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012024
VERSION     KHUT01012024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           58 c           94 g           38 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtcttg
       61 ccagtctgat gtgaaatctc ccggctcaac cgggagcagc cattggaaac tacaagactg
      121 gagtctggca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ccagtactga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012025
VERSION     KHUT01012025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agagcatgta ggcgggtatt
       61 taagtcagct gtgaaaacta tcggctcaac cggtagactg cagttgatac tggatatctt
      121 gagggtatga gagggcagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actgcctggc atacaactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012026
VERSION     KHUT01012026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggaatca ctgggcttaa agagcacgta ggcggtcctg
       61 taagcgtctt gtgaaatccc tcggctcaac cggggaattg cttggcgaac tgcaggactt
      121 gaggcaagta ggggtgcatg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccgga ggcgaaagcg gtgcactggg cttgccctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012027
VERSION     KHUT01012027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggcgatg
       61 caagtcaggg gtgaaatccc acagctcaac tgtggaattg cctttgaaac tgcattgcta
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012028
VERSION     KHUT01012028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           92 g           51 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggttttt
       61 caagtcctat gtgaaatctt ctggcttaac caggagcggt cgtaggaaac tgagggactc
      121 gaggcgatga gaggggtgca gaattccagg tgtagtggtg gaatgcgtag atatctggag
      181 gaataccagt ggcgaaagcg gcgccctggc atcggtctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012029
VERSION     KHUT01012029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           97 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agagcccgca ggcggcttgg
       61 taagttttgg gtgaaatctc ccggctcaac tgggagggcg cccggaaaac taccaggctt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012030
VERSION     KHUT01012030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtatg
       61 taagtcagtg gtgaaatctc caagcttaac ttggaaactg ccgttgatac tatatatctt
      121 gaatatcgtg taggtaagcg gaatgtgtca tgtagcggtg aaatgcatag atatgacacg
      181 gaacaccgat tgcgaaggca gcttacttta cgatcattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012031
VERSION     KHUT01012031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggcttta
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cattcgatac tgtttagctt
      121 gagtgcggga gaggttacta gaattcttgg tgtagtggtg aaatacgtag atatcaagag
      181 gaataccgga ggcgaaggcg ggtaactggc ctagcactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012032
VERSION     KHUT01012032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           89 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtgggtgct
       61 taagtctggt ttgaaagctg gttgctcaac aatcagatgt ggctggaaac tgggcgtctt
      121 gaatgggttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012033
VERSION     KHUT01012033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggcttg
       61 taagtcagtg gtgaaatctc cgtgcttaac acggaaactg ccattgatac tataggtctt
      121 gaatgttctg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctaactaca gggttattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012034
VERSION     KHUT01012034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           94 g           41 t
ORIGIN      
        1 tacagaggcc ccgagcgcta atcggaatta ctgggcttaa agggtgcgta ggcggatccg
       61 caagcgtctt gtgaaagccc tcggctcaac cgaggaacag cgaggcgaac tgcgggtctt
      121 gaggcaggta tgggtcgccg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg ggcgactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012035
VERSION     KHUT01012035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           91 g           48 t
ORIGIN      
        1 gacgaacctg acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaatag cgcttgagac tgccgtgctt
      121 gagggagata gaggtgagcg gaacctaggg tggagcggtg aaatgcgttg atatcctagg
      181 gaacaccagt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012036
VERSION     KHUT01012036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c           97 g           44 t
ORIGIN      
        1 tacgaatcgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccgg
       61 ccagtccgtg gtgaaatctg tcggctcaac cggcagactg cctcggatac tgccgggctc
      121 gagggtgata ggggcgtgcg gaacgagtgg tggagcggtg aaatgtgttg atatcactcg
      181 gaactccggt ggcgaaggcg gcacgctggg tcacctctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012037
VERSION     KHUT01012037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           96 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtttgt
       61 cgcgtctgcg gtgaaagtct gaggctcaac cttggaattg ccgtgggtac gggcagacta
      121 gagtgctgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg cagtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01012038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012038
VERSION     KHUT01012038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           84 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggacctt
       61 taagtagaaa gtgaaaggtt ggagctcaac tccgacactg ctctctatac tgggggtctt
      121 gagtcttgga gggggaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca gcttcctgga caagtactga cgctcaggca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01012039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012039
VERSION     KHUT01012039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttga
       61 tcagtcagat gtgaaatccc tcggcttaac cgaggaactg catctgatac tgtcaggcta
      121 gagtgtcgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatccgt ggcgaaggcg gccatctgga agacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012040
VERSION     KHUT01012040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgca ggcggtttgg
       61 taagttgggt gtgaaagccc tcggctcaac cgaggaagtg cattcaaaac taccaagctt
      121 gagtaccgga gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctgga cggatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012041
VERSION     KHUT01012041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           52 c           76 g           44 t
ORIGIN      
        1 tacgtaggga gcaaacgttg tccggattta ttggacgtaa agagcgtgta ggcggccaga
       61 caggtccatt atgaaaactc gaaactcaac ctcgagacgt cgacggaaac cgtctggcta
      121 gagtccggaa gaagagtgtg gaattcctag tgtaacggtg aaatgcgcag atatcaggaa
      181 gaacacctat ggcgaaagca gcactctggg acgatactga cgctgagacg cgaaaacgta
      241 aagagcgaac agg
//
LOCUS       KHUT01012042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012042
VERSION     KHUT01012042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac taccatgctg
      121 gaggaagaca gaggtgagcg gaacttaggg tggagcggtg aaatgtgttg atatcctaag
      181 gaacaccggt ggcgaaggcg gctcactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012043
VERSION     KHUT01012043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtcttgt gtgaaatcct tgggcttaac tcaagaagtg cgcaagaaac ggtaagacta
      121 gagtcctaga gaggctagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gctagctgga taggcactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012044
VERSION     KHUT01012044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           79 g           59 t
ORIGIN      
        1 tacgtagggt cctagcgttg tccggattta ctgggtgtaa agggtgcgca ggcggactga
       61 taagtcaggg gtgaaatctc acagctcaac tgtgaaactg cctttgatac tgtcagtctt
      121 gaataagcga gagattcgtg gaattccgag tgtagcagtg aaatgtgtag atattcggaa
      181 gaacaccagt ggcgaaggca gcgaattggc gctttattga cgctcaggca cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01012045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012045
VERSION     KHUT01012045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggg
       61 caagtctgcg gtgaaatcct ccagcttaac tggagaagtg ccgtggatac ttcttacctg
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccgat ggcgaaggca aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012046
VERSION     KHUT01012046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggtggctttg
       61 tgtgtctggt gtgaaatccc ttggctcaac caaggaagtg cgccagaaac tgcaatgctc
      121 gagtttggga gaggatggta gaattctcag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccggt ggcgaaggcg accatctgga ccaatactga cactgatgtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012047
VERSION     KHUT01012047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtttgg
       61 taagtccgtt ctgaaagccc agggctcaac cctgggaggc ggatggatac tgtcaaactc
      121 gagtccggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012048
VERSION     KHUT01012048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaatta ctgggcgtaa agggtgtgta ggtggcgtat
       61 taagtctgtt gttaaatctc ctggctcaac cgggaaatcg cagtggaaac tggtatgcta
      121 gagggtgaga gagaggagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gctccttggc tcacttctga cactgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012049
VERSION     KHUT01012049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggttcgc
       61 tatgtcggat gtgaaagccc tcggcttaac cgaggaaggg catccgaaac tggcgagctt
      121 gagtacggaa gagggtctcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gagacctggg ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012050             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012050
VERSION     KHUT01012050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           83 g           56 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ctgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtctttt gttaaattct tcggctcaac cgggggcatg caaaagaaac ggcactgact
      121 agaggatacg aggggtctgt ggaactcatg gagtaggggt gaaatccgtt gatatcatgg
      181 ggaacaccga aagcgaaggc agcagactgg cgtattcctg acactgaaac acgaaagcgt
      241 aggtagcgaa tggg
//
LOCUS       KHUT01012051             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012051
VERSION     KHUT01012051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           49 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatca ttgggcgtaa agggctcgta ggcggacttg
       61 caagtttgac gtgaaatctc caccgctcac cggggagagg tcgttgaata ctacaagtct
      121 ggaattcggt agaggatcgt ggaatttcgg gtgtagcggt ggaatgcgta gatatccgaa
      181 agaacaccag tggcgaaggc gacgatctgg gcctgaattg acgctgagga gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012052             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012052
VERSION     KHUT01012052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           54 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagctcgta ggcggcggcg
       61 taagtgggag gtgaaatcct atcggctcaa ccgtggaatt gcctttcata ctgcgtcgct
      121 tgaggaaggt aggggcaagt ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc gacttgctgg atcttttctg acgctgaggg gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012053
VERSION     KHUT01012053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           92 g           48 t
ORIGIN      
        1 tacagaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccatt
       61 taagtcagat gtgaaagccc ggggcttaac ctcggaactg catttgaaac tgagtggcta
      121 gagtatggta ggggagaggg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg cctctctggg ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012054
VERSION     KHUT01012054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          103 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcctgta ggcggaagtt
       61 taagcggtgc ctgaaagccc ccggctcaac cggggagggt ggtgccgaac tgggcttctt
      121 gagggcgata gaggaaggtg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccttctggg tcgaccctga cgctgagagg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012055
VERSION     KHUT01012055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcac
       61 caagtcgggg gtgaaatttt tcggctcaac cggaaaagtg ccttcgatac tggtggacta
      121 gagcgaggta ggggcggatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012056
VERSION     KHUT01012056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           44 c          102 g           56 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggtggtttgt
       61 cgcgtcgact atgaaagctc ctggcttaac tgggagtgtg tggtcggtac gggcagactt
      121 gagtgcagta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcagggg
      181 gaacaccggt ggcgaaggcg ggtctctggg ctgttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012057
VERSION     KHUT01012057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           81 g           52 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggcggttatg
       61 aaagttagaa gtgaaagccc agggcttaac cctggaattg cttttaaaac tccataactc
      121 gagtactgga gaggttggcg gaattccgag tgtagcagtg aaatgcgtag atattcggag
      181 gaacaccgat ggcgtaggca gccaactgga cagttactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012058             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012058
VERSION     KHUT01012058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c          100 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctctt
       61 taagtctgat ggttaaatgc cggggctcaa ccccgagtgc gccagagata ctggggagct
      121 agagacaggt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcctggtctg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012059
VERSION     KHUT01012059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           94 g           43 t
ORIGIN      
        1 gacgaaccgc ccgaacgtta ttcggaatca ctgggcttaa agggagcgta ggcgggccgc
       61 caagtccggg gtgaaatcct ccagcttaac tggagaactg cctcggatac tggaggtctc
      121 gaggagggta ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg atatccatag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccctttctga cgctgaggct cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012060             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012060
VERSION     KHUT01012060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta cttgggcgta aagggcgtgt aggcggttgg
       61 ataagttgga tgtgaaagac tggggcttaa cctcggggtt gcatacaaaa ctgtcttact
      121 cgagtgcagt agagggaggt ggaatttccg gtgtagcggt gaaatgcgta gatatcggaa
      181 ggaacatcag tggcgaaggc gacctcctgg actgacactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012061
VERSION     KHUT01012061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           88 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttgaa
       61 taagttatct gtgaaatccc tgggcttaac ctgggcaggt cagataagac tgtttgactc
      121 gagtatggga gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctatctggc ctaatactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012062
VERSION     KHUT01012062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgagaggc ccaaacgtta ttcggattta ctgggcttaa agagttcgta ggcggtccag
       61 aaagttgggt gtgaaagccc tcggctcaac cgaggaattg cgctcaaaac ttctggactt
      121 gagggagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012063
VERSION     KHUT01012063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggaaggt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcgaac tggctttctt
      121 gagagcggca gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcactctggg ccggctctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012064             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012064
VERSION     KHUT01012064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggccagc
       61 acaagtcggg tgtgaaagcc ctgggctcaa cctaggaagt gcactcgaaa ctgtgcggct
      121 tgaagtctgg agaggaaggc ggaattctcg gtgtagaggt gaaattcgta gatatcgaga
      181 ggaacaccgg tggcgaaggc ggccttctgg acagcacttg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012065
VERSION     KHUT01012065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggag
       61 caagtgaggt gtgaaatccc ggagctcaac tctggaactg cattttatac tacttcgcta
      121 gagtcttgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga caaagactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012066
VERSION     KHUT01012066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           62 c          106 g           36 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcccct
       61 caggcacgac gtgaaagccc cgggctcaac ccgggagggt cgtcgtgaac cgtggggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtactga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012067
VERSION     KHUT01012067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 gacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtacttt gtgaaatccc acggctcaac cgtggaactg ctgagtatac tggcgatctt
      121 gggccattca ggggttgccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggcaactggg gatgtgccga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012068
VERSION     KHUT01012068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           63 c          102 g           33 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagcgcccc gtgaaatccc ccggctcaac cggggaacgg cggggcggac tggcggcctt
      121 gaggcaccca ggggtcagcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgac ggcgaaagca gctgactggg ggtgttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012069
VERSION     KHUT01012069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggtcatt
       61 taagtcggat gtgaaagccc tcggctcaac tgaggaagtg catccgaaac tgaatagcta
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012070
VERSION     KHUT01012070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           79 g           58 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtccgca gccggcgtgt
       61 taagtgagat gttaaatcgt gtggctcaac catatcgatg tatctcatac tggcatgcta
      121 gaggaaatca gaggcaagtg gaatttgtag tgtagcagtg aaatgcgtag atattacaag
      181 gaacaccaat ggcgaaggca gcttgctggg atttttctga cggtcatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012071
VERSION     KHUT01012071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           99 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtctggg gtgaaagcct ccggcctaac cggagaagag cttcagatac tggtcgcctc
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012072
VERSION     KHUT01012072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggactgc
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaaggg ccttggaaac tggcggtctc
      121 gagggaggta ggggcctgtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg acgggctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012073
VERSION     KHUT01012073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           90 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcccgc
       61 taagtcggat gtgaaagccc aaggctcaac cttggaagtg cattcgaaac tggcgagctt
      121 gaatatggac gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatattga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012074
VERSION     KHUT01012074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           87 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtgata
       61 tttgtctggg gtgaaatccc tgagcttaac tcaggaagtg ccttggaaac ggtatcactc
      121 gagtattgga gaggttcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatcaga ggcgaaggcg acgaactgga caataactga cactaaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012075
VERSION     KHUT01012075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agagaacgta ggtggctttc
       61 taagattgat cttaaagacc ggggctcaac cccgggactg gatcgataac tgggaggcta
      121 gaggatgtca gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccagt ggcgaaagcg actttctggg gcattcctga cactgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01012076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012076
VERSION     KHUT01012076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           86 g           56 t
ORIGIN      
        1 tacggaggga actagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggtttat
       61 taagttggga gtgaaatccc agggctcaac cctggaactg ctctcaaaac tggtagacta
      121 gagtgtgata gggggtgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacatcggt ggcgaaggcg atcacctgga tcattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012077
VERSION     KHUT01012077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggagcct
       61 caagctggat gtgaaagacc tgggcttaac ctgggggtgt cattcataac tgaggttcta
      121 gagtatggga gaggagagtg gaattcacag tgtagcggtg aaatgcgtag atattgtgag
      181 gaacatcgat ggcgaaggca gctctctggc ctgatactga cgctgaggcg cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012078
VERSION     KHUT01012078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           76 g           64 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaatga
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaattg ccgttgatac tgttgttctt
      121 gagtatattt gatgtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcacaaaa ctataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012079             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012079
VERSION     KHUT01012079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           65 c           99 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta gacgggccac
       61 cgcgtcgacg gctgaaagcc cccggctcaa ccggggaagc ggcctcgata cgggtggcct
      121 cgagtggagg agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg actcccactg acgttgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012080
VERSION     KHUT01012080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           44 c           88 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttgt
       61 taagtcggcg gtgaaagtct gtggctcaac catggaattg ccgttgatac tggcgggctt
      121 gagtgttggt ggggtacatg gaattggtgg tgtagcggtg aaatgcatag ataccatcag
      181 gaacaccgat agcgaaggca ttgtacttac caacaactga cgctgaggca cgaaagcgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01012081             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012081
VERSION     KHUT01012081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           52 t
ORIGIN      
        1 gacgaactgt gcgaacgtta ttcggagtca ctgggcttaa agcgctcgta ggcggcatcg
       61 taagtcagag gtgaaataca atcggctcaa ccgttgaatt gcctttgata ctgcggagct
      121 agagaaaaat aggggcacat ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccgg tggcgaaagc gatgtgctgg gtttttactg acgctgagga gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012082
VERSION     KHUT01012082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtaagt
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaactg cattggaaac tggcttgctg
      121 gagtgtggca gaggggggtg gaattccttg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ccaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012083             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012083
VERSION     KHUT01012083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           85 g           55 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agagtgcgta ggtggtttaa
       61 taagttatcg gtgggaaatc ggggctcaac cccgtggaag tcggtaatac tgttagacta
      121 gaggactgga gaggttaacg gaatggttca tgtaggggta aaatccacag atatgaactg
      181 gaacaccgaa cggcgaaggc agttaactat tcggttcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01012084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012084
VERSION     KHUT01012084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           92 g           52 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgtgta ggcggcttga
       61 caagtagggt gtgaaaggcc tcggctcaac cggggcattg cgctctagac tgtctagctc
      121 gagcaagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttgtctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012085             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012085
VERSION     KHUT01012085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           61 c           84 g           44 t
ORIGIN      
        1 caccagcgcc acgagtggct atcacgatta ttgggcctaa agcgtccgta gtcgggcaag
       61 tacatcttct gtgaaatcgc tgcgcttaac gtagcggcat gcggaagaca cggcttgtct
      121 tgagatcggg aggcgtcagg ggtactcctg ggggaccggt aaaatggtat aatcccagga
      181 agaccacctg tggcgaaggc gcctgacgag aacgaatctg acgatgaggg acgaaagcca
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01012086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012086
VERSION     KHUT01012086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           88 g           49 t
ORIGIN      
        1 gacgaaccgc ccgaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggcgctg
       61 taagtcgggt gtgaaatcct tcggcttaac cgaagaattg cgcccgaaac tgcagtgctc
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012087
VERSION     KHUT01012087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           88 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggtttgg
       61 taagtctctt gtgaaagctc ctggctcaac tgggagaggt caagggaaac taccaggcta
      121 gagtgcggaa gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggcttctggt ccgtcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012088
VERSION     KHUT01012088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           88 g           56 t
ORIGIN      
        1 tacagggggt gcaagcgtta ttcggaatta ttgggcgtaa agagcgtgta ggtggtttac
       61 taagtttggt gtgaaagccc tgggctcaat ccaggaagtg cattgaatac tggtagactt
      121 gagtacagga gaggagagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gctctctgga ctgttactga cactaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012089
VERSION     KHUT01012089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           44 c           80 g           50 t
ORIGIN      
        1 tacgtagggc gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggagagg
       61 aaagtcagat gtgaaatgcc aaggctcaac cttggaacag catatgaaac tctcactcta
      121 gaggataggc ggagaaaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttatacctga cgctgaggcg cgaaagttag
      241 gggagcaaac agg
//
LOCUS       KHUT01012090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012090
VERSION     KHUT01012090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           84 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggtttga
       61 taagttaggt gtgaaagtcc tgggcttaac ctgggaattg cgcctaatac tgtcaagcta
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg accttctgga cagatactga cactgagaca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012091
VERSION     KHUT01012091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          101 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 gaggtcgggg gtgaaatccc acggcttaac cgtggaaggg ccttcgatac cggcgggctg
      121 gaggaaggta ggggtgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggc ggcgaaagcg gcgcactgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012092
VERSION     KHUT01012092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           53 c           81 g           43 t
ORIGIN      
        1 tacaaaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 caagtccgcc gtcaaatccc agggctcaac ccaggacagg tggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttccag tggaacggtg aaatgcattg agatcagaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01012093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012093
VERSION     KHUT01012093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacagggggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggcctcg
       61 tatgtcttct gtgaaagccc tcggctcaac cgaggaactg caggagaaac ggcgaggctc
      121 gagtccggga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccggtactga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012094
VERSION     KHUT01012094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggtatga
       61 caagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac tgtcggacta
      121 gagtttggtt ggggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ctaacactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012095
VERSION     KHUT01012095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggctgactcg
       61 tgtgtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac tgcgagtcta
      121 gagtggtgga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012096
VERSION     KHUT01012096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           99 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggactgc
       61 caagtccggg gtgaaatcgt tcggctcaac cggacaatag cctcggagac tggcggtctg
      121 gaggggggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012097
VERSION     KHUT01012097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           60 c          104 g           42 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtaa agggcgcgca ggcggtcacg
       61 ggcgtcggtg ctgaaagtgc gccgcttaac ggcgcaatgc gtgccgatac gctgtgactg
      121 gagcgcgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag atatcggcag
      181 gaacacctgt ggcgtaggcg gccacctagc ccgctgctga cgctgaggcg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01012098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012098
VERSION     KHUT01012098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           85 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggctgatatg
       61 taagttagat gtgaaatgcc tgggcttaac ctaggaacgg catttaagac tgcaagtcta
      121 gagtttgtca gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca gccccctggg atagaactga cgctcatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012099
VERSION     KHUT01012099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 gacgagtccg gcaaacgtta ttcggaatta ctgggcttaa agcgtgcgta ggtggtctga
       61 agggttggga gtgaaatccc acggcctaac cgtggaattg ctctcaaaac cgttagacta
      121 gaggaggtta gaggcgagcg gaactacggg tggagcggtg aaatgcgttg atatccgtag
      181 gaacaccggt ggcgaaggcg gctcgctggg gcctttctga cactgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012100
VERSION     KHUT01012100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          100 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcttg
       61 ccggtccggg gtgaaagccc ccagcttaac tggggaagcg ccttggatac cgcaagtctg
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012101
VERSION     KHUT01012101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           90 g           51 t
ORIGIN      
        1 gacgaactgc ccaaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcggcttac
       61 caagtcaggg gtgaaatccg gcagcttaac tgtcgaagtg cctttgatac tggtaagctc
      121 gagggaggta ggggtccgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga tcttttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012102
VERSION     KHUT01012102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcta
       61 caagtcagga gtgaaatccc caggctcaac ctgggaatgg cttttgagac tgtagggctt
      121 gagtacagga gaggtgagtg gaattcccag tgtagaggta aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012103
VERSION     KHUT01012103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtggaa
       61 taagtcggat gtgaaatctc ggggcttaac cccgaaactg cattcgatac tgtttcactc
      121 gaggactgga gaggagattg gaattcatgg tgtagcagtg aaatgcgtgg atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012104
VERSION     KHUT01012104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           96 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcgggttac
       61 caagtggtgt atgaaaggtt ggggcttaac tccaacagtg tatgccagac tgatagcctg
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012105
VERSION     KHUT01012105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           79 g           54 t
ORIGIN      
        1 tacgagaacc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggtttgg
       61 ttagtcttct gttaaaactt cgggcttaac ccgaagcttg cagtggaaac ggccaaactc
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggta aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcattcctga cactgagaca cgaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01012106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012106
VERSION     KHUT01012106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           79 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgca ggtggattag
       61 taagtcagtg gtgaaagcct tgagcttaac tcgagaactg ccattgatac tattagtctt
      121 gaatgttgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaaa caaatattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012107
VERSION     KHUT01012107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           91 g           43 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggccgat
       61 taagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cacacgaaac tgaccggctt
      121 gaatatggaa gagggttgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcaacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012108
VERSION     KHUT01012108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           51 t
ORIGIN      
        1 cacgggggga acgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcgggtcgg
       61 caagtcgggt gtgaaattcc atggcttaac catggatctg cattcgaaac tgccggtctt
      121 gagtactgga gaggtgagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgtt ggcgaaggcg gctcactgga cagatactga cgctgaggca cgaaggtcgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012109
VERSION     KHUT01012109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           80 g           55 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggaatca ttgggtgtaa agggcaagta ggtggtttgt
       61 taagtgaggg gtgaaatcct gcgactcaat cgcagaactg cctttcaaac tggcagactt
      121 gagggtggta gaggaaaatg gaattctcgg tgtaagggtg aaatctgtag atatcgagaa
      181 gaacaccagc ggcgaaggcg attttctgga ccactcctga cactaaattg cgaaagctag
      241 gggagcaaac aga
//
LOCUS       KHUT01012110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012110
VERSION     KHUT01012110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggtttaa
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaatgg cgttgaagac tgttagactg
      121 gagtcggaca gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacacccgt ggcgaaggcg acagcctggg tccagactga cactgagggg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012111
VERSION     KHUT01012111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          103 g           37 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatca ctgggcgtaa agggcgtcta ggcggcacgc
       61 caagtcttgc gtgaaagctc ctggctcaac taggagagtg cgtgggaaac tggcgagcta
      121 gagacgtgga ggggtgcacg gaattcccgg tggagtggtg gaatgcgttg agatcgggag
      181 gaacaccaga agcgaaggcg gtgcactggc cacggactga cgctgaagcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012112             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012112
VERSION     KHUT01012112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           42 c           77 g           55 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta ggtggttcaa
       61 taagttactg gtgggaaatc ggggctcaac cccgtggaag tcagtaatac tattaaacta
      121 gaggatcgga gaggttaacg gaactccatg tgtagcggta aaatgcataa atatatggat
      181 gaacaccaaa cagcgaaggc agttaactat tcgattcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01012113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012113
VERSION     KHUT01012113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           81 g           54 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggattaa ttgggcgtaa agggtacgca ggcggttcgt
       61 taagtcaact gttaaatctc ccggcctaac tgggaatcag cggtagatac tggcgagcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagaggta aaattcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgctcaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012114             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012114
VERSION     KHUT01012114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           80 g           57 t
ORIGIN      
        1 tcccggcagt ccaagtcgca gccacatttg ttgggtctaa aatatccgta gcttgccttt
       61 caagtctttt gtgaaatcgg gactcttaaa gttccggcgg gcaaaagata ctgtaaggct
      121 agggaccggc aggcgcaaaa agtacagaaa gggtagcggt aaaatgtgtt gatcctttct
      181 ggactaacag tcgcgaaggc gttttgcgag gacgggtccg acagtgaggg atgaaggcta
      241 ggggcgcaaa gtgg
//
LOCUS       KHUT01012115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012115
VERSION     KHUT01012115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          103 g           40 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggcggctcgg
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtctg ccgtgcgaac tgccgggctc
      121 gagcacagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag atatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctgttgctga cgctgaagca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012116
VERSION     KHUT01012116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           91 g           52 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ttgggcttaa agcgcacgta ggcggctctt
       61 caagtgtctt gtgaaatccc tcggctcaac cgaggaattg ctgggcatac tgtcgagctt
      121 gaggtaggta tgggcgagtg gaactcttgg tggagcggtg gaatgcatag atatcaagag
      181 gaacgccggt ggtgaagacg actcgctggg cctatcctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012117
VERSION     KHUT01012117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           47 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggttcgg
       61 taagtctgtt gtgaaagctc ctggctcaac tgggagaggc caacggaaac tgccggactt
      121 gagtatagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtgcctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012118
VERSION     KHUT01012118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           97 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgta ggcggtcatt
       61 taagtctgac gtgaaagctc ctggcttaac tgggagagga cgttggagac tggatggctt
      121 gaggcgatga gaggggcgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcgtcctagc atcggcctga cgttcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012119
VERSION     KHUT01012119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 taagtccggg gtgaaatcct ccagctcaac tggagaagtg ccttggatac tgctggtctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcatctga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012120             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012120
VERSION     KHUT01012120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           74 g           60 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtaaaa
       61 taagttgttg gtgaacgtca agctcaactt gactaaagcc agtaatactg ttttgctaga
      121 gaatgggaga agttgatgga actctacgag gaagggtgaa atctgttgat ccgtagcgga
      181 ccaccaaaaa aggcgaaggc aattaactat tccatttctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01012121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012121
VERSION     KHUT01012121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtcgaa
       61 taagtgtggt gtgaaatccc cgggcttaac ctgggaagtg catcgcaaac tgattgacta
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012122
VERSION     KHUT01012122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           88 g           53 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggatat
       61 taagtcagat gtgaaaaacc gaggctcaac ttcgggactg catctgaaac taatattctt
      121 gagtgcagga gaggaaagcg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012123
VERSION     KHUT01012123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           84 g           50 t
ORIGIN      
        1 tacgaggacc ccgagcgtta tccggattta ttgggcgtaa agagtgcgca ggtggtttgg
       61 tgcgtctgtc gttaaatgtc actgctcaac agtgtcaccg cgggagatac gaccaaacta
      121 gagcgtgtaa gaggtagctg gaactcacgg tgtaggggtg acatccgttg atatcgtggg
      181 gaacaccaaa ggcgaaggca ggctactggt acatcgctga cactcaggca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01012124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012124
VERSION     KHUT01012124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           94 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcgggtttg
       61 tatgtcgatt gtgaaagccc tgggcttaac ccgggaattg cagtcgaaac tgcaaatctt
      121 gagtgcttga gggggtggcg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacacctga ggcgaaggcg gctgcctggc aagacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012125
VERSION     KHUT01012125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           92 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtttat
       61 agagttagct gtgaaattcc tgggcttaac ctggggtggt cagctaatac tggtagactg
      121 gagtacgaga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actacctggc tcgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012126
VERSION     KHUT01012126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggattca ttgggcttaa agcgtgcgta ggcggatatg
       61 caggcgtcgt gtgaaagccc tcggctcaac cggggaactg cacggcgaac cgcatgtctt
      121 gagacaagta ggggcggccg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggccgctggg cttgttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012127
VERSION     KHUT01012127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           89 g           51 t
ORIGIN      
        1 tacgagtgcc tcaagcgtta tccggaatca ttgggcgtaa agggtgcgta ggcggcgatg
       61 ttagtctcgc gttaaattct ccggctcaac cggggagctg cgtgggaaac ggcattgctt
      121 gaggacggaa ggggtctctg gaactcatgg tgtagcggtg aaatgcgttg atatcatggg
      181 gaacaccaaa agcgaaggca agagactggt ccgctcctga cgctgaagca cgaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01012128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012128
VERSION     KHUT01012128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           85 g           55 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggagctt
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaattg cattcgatac tggggctcta
      121 gagtatagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcttcctgga ctaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012129
VERSION     KHUT01012129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           84 g           56 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggcgatt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg ccttcgatac tattttgcta
      121 gagtatggaa gaggtaagtg gaactgcaag tgtagaggtg aaattcgtag atatttgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccattactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012130
VERSION     KHUT01012130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           81 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggctgg
       61 taagtctgtg gtgaaatccc cgagcttaac ttgggaactg ccatggatac tatcagtctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcatag atatgacata
      181 gaacacccat tgcgaaggca gctgactacg cggagattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012131
VERSION     KHUT01012131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           77 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatcct gtagcttaac tacagaactg ccattgatac tatttgtctt
      121 gaataccctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctcactacg ggaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012132
VERSION     KHUT01012132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggctggc
       61 tcagtcggac gtgaaatccc caggctcaac ctgggaactg cgtccgatac tggccggctt
      121 gaatccggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccggcattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012133
VERSION     KHUT01012133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           87 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggatcaa
       61 taagttgggg gtgaaagccc agagctcaac tctggaactg ccttcaaaac tgttgatctg
      121 gactcaagga gaggcaagtg gaatgtctag tgtagaggtg aaattcgtag atattagacg
      181 gaacaccagt ggcgaaggcg acttgctgga cttgtagtga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012134
VERSION     KHUT01012134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgatg
       61 caagtcaggt gtgaaatgcc ggggctcaac cctggaacgg catccgatac tgcattgcta
      121 gagtagtgga ggggcgactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactagt ggcgaaggcg agtcgctgga cactaactga cgctgaggca cgaaggccag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01012135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012135
VERSION     KHUT01012135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggtggctaat
       61 taagtcaggt gtgaaagccc tcggctcaac cgaggaagcg catctgaaac tgattaactt
      121 gagtacggga gaggagagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg actctctgga ccgatactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012136
VERSION     KHUT01012136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           75 g           56 t
ORIGIN      
        1 tacgaggaga gcaagcgtta tccggattta ttgggcgtaa agcgtgcgca agctgttttg
       61 atagtgagat gttaaagccc aaagctcaac tttggaatcg catctcatac ttcaagacta
      121 gaagcaagaa gaggcaaacg gaattcccgg tgtagtagtg aaatgcgttg atatcgggag
      181 gaacaccaaa agcgaaagca gtttgctggt cttgtcttga cgccgttgca cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012137
VERSION     KHUT01012137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtctgt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaattg cattcgagac tggcaggcta
      121 gagtctggta gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg accacctgga ccaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012138
VERSION     KHUT01012138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           99 g           40 t
ORIGIN      
        1 cacgtaggat ccgagcgtta tccgaattta ctgggcgtaa agcgcatgca ggcggccggc
       61 taagttggac gtgaaagctc ctggctcaac tgggagaggt cgttcaagac tggcgggcta
      121 gagggcggca gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaagcg gcctcctggg ccgcgcctga cgctgagatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012139
VERSION     KHUT01012139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccact
       61 taagtccggt gtgaaagccc ggggctcacc cctggaagtg catcggatac tgagtggctc
      121 gagtaccgga gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012140
VERSION     KHUT01012140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           96 g           51 t
ORIGIN      
        1 tacggagggt ccaagcgttg tccggattta ttgggtgtaa agggtgtgta ggcgggcccg
       61 taagtcaggg gtgaaatcct gcggcttaac cgtagaactg cccttgatac tgcgggtctt
      121 gagtcctgga gaggtcgccg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacgccgga ggtgtagacg ggtgactgga caggaactga cgctaaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012141
VERSION     KHUT01012141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgcat
       61 taagtcaggt gtgaaagccc tgggctcaac ccaggaatag cacttgatac tgatgcgctt
      121 gaattcggga gaggatgacg gaattcccag tgtagaggtg aaattcattg atattgggaa
      181 gaacaccagt ggcgaaggcg gtcatctggc ccgtaattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012142
VERSION     KHUT01012142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcgtcg
       61 caagtcaaag gtgaaatccc tcggctcaac cgaggaactg cctttgaaac tgcgacgctt
      121 gaggccggga ggggccagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctggctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012143
VERSION     KHUT01012143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacagagatg gcaagcgttg ttcggaatca ctgggcataa agggcacgta ggcgggctgg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaattg cgcctgatac tgccagtctt
      121 gagggcagga ggggagagtg gaactcttgg tggagcggtg aaatgcgtag ataccaagag
      181 gaacgccggt ggcgaaagcg actctctggc ctgcttctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012144
VERSION     KHUT01012144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           77 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggactga
       61 taagtcaggg gtgaaagccg gtagctcaac tatcgaatag cctttgaaac tgccagtctt
      121 gaatattgtt gaggtaggcg gaatgtatca tgtagcggtg aaatgcatag atatgataca
      181 gaacaccgat tgcgaaggca gcttactaag caattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012145
VERSION     KHUT01012145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c          101 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggtggggtgc
       61 caagcggggt gtgaaagctc tcggcttaac tgagagagtg cgctccgaac tggcaagcta
      121 gagggcaggc gacgtggatg gaatacccgg tggagtggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gtccacgagc ctgtgcctga cactgaagcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012146
VERSION     KHUT01012146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           63 c          104 g           34 t
ORIGIN      
        1 gacggagggc gctagcgttg ttcggaatca ctgggcgtaa agggcgcgca ggcggcccgg
       61 cgagtcgggc gtgaaagccc ggggctcaac cccggaacgg cgtccgatac tgccgggctc
      121 gaggccggga gagggtggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gccacctggc ccggtcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012147
VERSION     KHUT01012147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           67 c          113 g           27 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggccccg
       61 caggcggccc gtgaaagccc ccggctcaac cggggggggt cgggtcgaac cgcggggctg
      121 gagaccggta gaggccggtg gaattcccgg tggagcagtg aaatgcgtag agcccgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ccgggtctga cgctgaggcg cgaaggccgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012148
VERSION     KHUT01012148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggtggtgact
       61 caagtcggat gtgaaagccc ttggcttaac cgaggaagtg catccgaaac tgagtcgcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012149
VERSION     KHUT01012149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           81 g           58 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggttgttcta
       61 caagctagtg gtgaaagccc agggcttaac cctggaattg ccattagaac tgtagagcta
      121 gagagtgtta gaggtaagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacatcaat ggcgaaggca gcttactggg tcacatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012150
VERSION     KHUT01012150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           42 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcccgg
       61 ccagtcaaag gtgaaatccc tcggctcaac cgaggaactg cctttgaaac tgctgggctt
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012151
VERSION     KHUT01012151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c          101 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggagtta ctgggcgtaa agcgtatgta ggcggttggg
       61 caagtggcgt gtgaaagcac ccggctaaac tgggtgagga caggtcagac tgctcgacta
      121 gagggtggta gaggagcgtg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggcgcaagcg gcgctctggg ccactactga cgctgagata cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012152
VERSION     KHUT01012152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          101 g           41 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgca
       61 gcagtcaggc gtgaaagccc cgggctcaac ctgggaattg cgcttgagac gctgtggctg
      121 gaggtcggga gaggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctggc ccgcacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012153
VERSION     KHUT01012153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           88 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcgggtact
       61 taagtcaggg gtgaaagact atcgcttaac gatggcagtg cctttgatac tgagtgcctt
      121 gagtacagat gaggctggcg gaatgggtgg tgtagcggtg aaatgcatag ataccatcca
      181 gaacaccgat tgcgaaggca gccggctaaa ctgctactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01012154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012154
VERSION     KHUT01012154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           85 g           54 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcagaatta ctgggcgtaa agagtgcgta ggcggatata
       61 caagttagat gtgaaactcc atggctcaac cgtgggacgg catttaagac tgtatgtcta
      121 gagtttgtta gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca gccccctggg ataagactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012155
VERSION     KHUT01012155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggattta ctgggcgtaa agcgcacgta ggcggctcgg
       61 aaagttagac gtgaaagctc ccggcttaac tgggagaggt cgtttaatac taccgagctt
      121 gaggttgaaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tcagacctga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012156
VERSION     KHUT01012156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           77 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggcgggggtt
       61 taagtcagtg gtgaaatccc gtagctcaac tacggaactg ccattgatac tgaatctctt
      121 gagtctggtt gatgtgaatg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccaat cgcgaaggca gttcacaaag ccagtactga cgctcaggca cgacagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012157
VERSION     KHUT01012157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           42 a           64 c          113 g           34 t
ORIGIN      
        1 tacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggaccat
       61 ctggcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac cggtggtctt
      121 gaggccggca ggggtcggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccgactggg ccgggcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012158
VERSION     KHUT01012158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggctcaa agggcgcgta ggcgggttcc
       61 acagtcgggg gtgaaatctt tcggcccaac cggaaaaggg cctccgatac tgggaacctc
      121 gagtggggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012159
VERSION     KHUT01012159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcctgt
       61 taagttggtt gtgaaaaccc atggctcaac catggaagtg cgtccaaaac tggcaggcta
      121 gagttctgac gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg aagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012160
VERSION     KHUT01012160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           62 c          109 g           33 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggccgag
       61 tatgtgcttg gtgacatccc acggctcaac cgtgggcggc ccaggtagac agctcggctg
      121 gagggtggga gagggtcgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcgacctggc ccatacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012161
VERSION     KHUT01012161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcggagaga
       61 taagtcgggc gtgaaatccc caggctcaac ctgggaactg cactcgaaac tgtctttctt
      121 gagtgccgga gaggaaagcg gaatacctgg tgtagaggtg aaattcgtag atatcaggtg
      181 gaacaccagt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012162
VERSION     KHUT01012162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatta ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cattcgatac tgcgaagctt
      121 gagtgccaga gaggttaata gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg gttaactggc tggacactga cactgagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01012163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012163
VERSION     KHUT01012163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattca ttgggcgtaa agagcgcgca ggcggcttgg
       61 taagtcggat gtgaaaatcc gaggctcaac ctcggacgtg catccgatac tgctcggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012164
VERSION     KHUT01012164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           87 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaga
       61 taagttgggt gtgaaattcc tgggctcaac ctggggactg cgctcaatac tgtttagcta
      121 gagtactgta gaggaaggtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012165
VERSION     KHUT01012165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           98 g           49 t
ORIGIN      
        1 gacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 ttagtcaggt gtgaaagccc tgggctcaac ctgggagtgg cacttgatac tggagggcta
      121 gagagtggga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012166
VERSION     KHUT01012166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           75 g           65 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggattaa
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaactg ccattgatac tgttaatctt
      121 gaatgtagtt gagatgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcgttaaa ctattattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012167
VERSION     KHUT01012167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          104 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 gaagtcagtg gtgaaagccc ggggctcaac ctcgggtcgg ccactgatac tctcgggctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012168
VERSION     KHUT01012168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          102 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcacgc
       61 caagtcaacg gtgaaagctc ggggctcaac ctcgagtcgg ccgtcgatac tggtgagctg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012169
VERSION     KHUT01012169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 aacgtagggg ccaagcgtta tccggattta ctgggcgtaa agcgcatgta ggtggttttt
       61 taagttggac gtgaaagctc ctggctcaac taggagaggt cgttcaaaac tggaaaactt
      121 gagggtggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccattcctga cactcagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01012170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012170
VERSION     KHUT01012170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgtgcgta ggcggctgag
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaattg cgctcaaaac tacttggctt
      121 gagagagtta gaggtgagcg gaactaatgg tggagcggtg aaatgcgttg atatcattag
      181 gaacaccggt ggcgaaagcg gctcactggg gctctactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012171
VERSION     KHUT01012171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           93 g           47 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctggg gtgaaagcct tgggcttaac ccaagaagtg ccccagaaac ggtgagactg
      121 gagtcctgga gaggtaagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcttactgga caggaactga cactgaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012172
VERSION     KHUT01012172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           49 c           98 g           50 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agggctcgca ggcggtctgt
       61 taagtctgac gtgaaatctc ccggctcaac tgggagggtg catcggatac tggcggactt
      121 gagggcatca gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg gtgcatctga cgctgaggag cgaaagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01012173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012173
VERSION     KHUT01012173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           84 g           51 t
ORIGIN      
        1 tacagagact ccaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggtcaat
       61 tgtgtcggag gtgaaatcct gcagcttaac tgtagatgtg ccttcgaaac tagttgacta
      121 gagtatcgga gaggcaagcg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccgaa ggcgaaggca gcttgctgga cgattactga cgctcaggca cgaaagcatg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01012174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012174
VERSION     KHUT01012174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggtcctt
       61 taagtcagcg gtgaaatccc acggctcaac cgtggaactg ccgttgatac tgagggactt
      121 gagttcgtcc gaggtcgctg gaatgcgtgg tgtagcggtg aaatgcatag atatcacgca
      181 gaacaccgat tgcgaaggca ggtgactggg acgcgactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012175
VERSION     KHUT01012175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           96 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgtc
       61 atagtcgggg gtgaaatctt tcggcccaac cggaaaaggg cccccgatac tggacgcctc
      121 gagggaggta ggggtcgatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012176
VERSION     KHUT01012176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           92 g           42 t
ORIGIN      
        1 gacggagggc gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggaaag
       61 taagtcagac gtgaaaactc ggggctcaac tccgagcctg cgtttgaaac tgctatcctt
      121 gagtgcagga gagggaagcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012177
VERSION     KHUT01012177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggggcgca ggcggtatgg
       61 taagtcagat gtgaaaaccc gaggctcaac ctcgggactg catttgaaac tgccagactt
      121 gagtgccgga gaggcaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttgctgga cggcaactga cgctgaggcc cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012178             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012178
VERSION     KHUT01012178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           84 g           53 t
ORIGIN      
        1 tacgtggggt gcgagcgtta tccggattta ctgggcgtaa agcgtaccgt cggcgattta
       61 gtaagttttc tgtccaagcc tggggcttaa ctccagaaag gcagagaaaa ctactagaat
      121 tgagtctgtt cgaggaacct ggaattctcg gtggaggggt gaaatccgta gatatcgaga
      181 ggaacgccaa gtgcgaaggc agggttctag gacacgactg acgctcatgg acgaaagcta
      241 ggggagcaaa aggg
//
LOCUS       KHUT01012179             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012179
VERSION     KHUT01012179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggctagg
       61 taagttttct gtgaaagcta cgggctcaac ccgttaaagt gcagagaata ctgcttggct
      121 cgagtgtcgg agaggatagt ggaattcccg gtgtagaggt gaaattcgta gagatcggga
      181 ggaacaccag tggcgaaggc gactatctgg ctgacaactg acgctgagac tcgaaagcat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012180
VERSION     KHUT01012180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 aacgtaggag cctagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggccgag
       61 taagtggatt gtgaaatctc ccggctcaac tgggagggga cggtccaaac tgctcggctg
      121 gaggcaggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcctcctgga cctgacctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012181             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012181
VERSION     KHUT01012181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c          102 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agagcgcgta ggcggcactg
       61 tacgaccgct gtgaaagccc ccggctcaac tggggagggt cagtggtaac ggcagagctc
      121 tgagtgcagg agagggaagt ggaactccgg gagtagcggt gaaatgcgta gagacccgga
      181 ggaacaccag tggcgaaggc ggcttcctgg tctgcgactg acgctgtggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012182
VERSION     KHUT01012182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           79 g           54 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggctttttgg
       61 aaagtctgag gttaaatttc ggggctcaac cccgtcaatg tcttggaaac tcccaaaatt
      121 gagcaatgga gaggtacctg gaatgccatg tgtaggagta aaatccgtag atatatggta
      181 gaacgccaaa agtgaagaca gggtactaga cattcgctga cgctgagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01012183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012183
VERSION     KHUT01012183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           81 g           54 t
ORIGIN      
        1 tacatggagt ccaaacgtta tccggattta ctgggcgtaa agcgttcgta ggtgttccat
       61 taagtttctg gtgaaatctc cgggcccaac tcggaaactg tcaggaaaac tagtggaatt
      121 gagtacggca gaggcagatg gaatgcttgg tgtaggggta aaatccgttg atatcaagca
      181 gaacaccagt ggcgaaggcg atctgctggg ccgatactga cactgaggaa cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012184
VERSION     KHUT01012184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           48 t
ORIGIN      
        1 gacgagctgt gcaaacgtta ttcggaatta ctgggcttaa agagttcgta ggcggcgcgt
       61 taggtggggt gtgaaatccc tcggctcaac cgaggaatgg cgctccaaac cggcgtgctt
      121 gaggaaggta gaggtgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccgga ggcgaaggcg gctcactggg ccttttctga cgctgaggaa cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012185
VERSION     KHUT01012185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           91 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgca gacggcagtg
       61 caagtctgaa gtgaaagctc gcggctcaac cgcggaactg ctttggaaac tgtacagcta
      121 gagtaccgga ggggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcttactgga cggtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012186             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012186
VERSION     KHUT01012186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           99 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcgatt
       61 attagtcagg cgtgaaagcc ccgggctcaa cctgggaggt gcgcttgaaa cgggatcgct
      121 agagttcggg agaggagagt ggaattccca gtgtagaggt gaaattcgta gatattggga
      181 agaacaccgg tggcgaaggc ggctctctgg accgaaactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012187             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012187
VERSION     KHUT01012187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           97 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggaccgg
       61 cacgtcagtc gctgaaatcc cccggctcaa ccggggaagt ggcgttgata cgaccggtct
      121 ggaggggggt aggggaatcg ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gcggttctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012188             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012188
VERSION     KHUT01012188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           75 g           57 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcata
       61 gtaagtgaat agtgaaagcg tgtggctcaa ccatacatcc attatttaaa ctactaagct
      121 agaagacgag agaggtagat ggaattcctg atgtaggggt aatatccgta gatatcagga
      181 ggaacaccga tggcgtaagc agtctactgg ctcgttcttg acactaaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012189
VERSION     KHUT01012189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacggggggg gctagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggcgcgg
       61 taagtcaaag gtgaaatccc tcggctcaac tgaggaactg cctttgaaac tgccgtgctt
      121 gagtccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012190             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012190
VERSION     KHUT01012190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agggcgcgta ggcggttacg
       61 taagtcggat gtgaaaactc caggctcaac ctggagacgc cacccgatac tgcgttgact
      121 tgagttcggt aggggatcac ggaatacctg gtgtagcggt gaaatgcgca gatatcaggt
      181 ggaacaccga tggcgaaggc agtgatctgg gccgatactg acgctgatgc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012191             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012191
VERSION     KHUT01012191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtctc
       61 taagtctggt gtgaaaggtt gagggctcaa ccccaacagt gcactggata ctgcggacct
      121 tgagttgggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctctctgg ccccaaactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012192
VERSION     KHUT01012192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcg
       61 caagtctgtg gtgaaagccc ccagcttaac tggggaagtg ccatggatac tgcgagtctc
      121 gaggagggta ggggcatctg gaacagtagg tggagcggtg aaatgcgttg atatctactg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012193
VERSION     KHUT01012193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           89 g           53 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatca ttgggcgtaa agcggacgta ggtggctttg
       61 taagtcaggc gtgaaagccc tgggctcaac ccaggaagtg cgcttgatac tgcttagctt
      121 gagtgtagga gaggtaagtg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg gcttactggc ctaacactga cgctgaggtc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012194
VERSION     KHUT01012194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           64 c          111 g           34 t
ORIGIN      
        1 cacgtagggg ccgagcgttg tccggaatta ctgggcgtga agcgcgcgca ggcggccatg
       61 cgcgcccggc gtgaaagccc ccggctcaac cggggagcgt cgttggggac ggcatggctt
      121 gagggcgaga ggggccggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccggctggc tcgactctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012195             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012195
VERSION     KHUT01012195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           77 g           63 t
ORIGIN      
        1 tacagaggtc acaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggtgggttt
       61 ataagttatc cttcaaagac tacggctcaa ccggaggaag ggggataata ctgtaagtct
      121 tgatttttgg tggggcatct ggaactgata gtgtagtagt gaaatacgtt gatattatca
      181 ggaactccaa gggcgaaggc aggatgctaa ccaattaatg acactgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01012196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012196
VERSION     KHUT01012196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctcgc
       61 taagtctggt gtgaaatccc caagcttaac ttgggaactg catcggaaac tggcgagctt
      121 gagtccggga gagggtagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012197
VERSION     KHUT01012197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          108 g           37 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccgc
       61 taagtctgct gtgaaaggcc ggggctcaac cccggaaggt cagcggagac tggtgggctg
      121 gagtgccgga gagggatgcg gaatacctgg tgtagcggtg gaatgcgtag agatcaggtg
      181 gaacaccgat ggcgaaggca gcatcctggc cggagactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012198
VERSION     KHUT01012198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           46 t
ORIGIN      
        1 tacgaaaggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 caagtgcggc gtgaaagtcc tgggctcaac ctggggacgg cgctgcagac tgctgggctt
      121 gagtccggaa gagggtggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccggt ggcgaaggcg gccacctggt ccggtactga cgctcaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012199
VERSION     KHUT01012199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggttatt
       61 caagtcagag gcgaaagccc ggggctcaac cccggaacgg cctttgaaac tagatagcta
      121 gagtcttgga gaggttagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actaactgga caagtactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012200
VERSION     KHUT01012200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           93 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccg
       61 taagtgcctt gtgaaatccc acggcttaac cgtggaactg ctgggcatac tgcgggtctt
      121 gagcggttta ggggctgtcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccgac ggtgaaaaca gacagctggg aacctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012201
VERSION     KHUT01012201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          104 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcctgca ggcggcaagc
       61 caagtcgtgc gtgaaatctc tcggctcaac tgagaggggt cgtgcgagac tggttcgctc
      121 gaggcgggta gggggaagcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcttcctgga cccggcctga cgctcagggg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01012202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012202
VERSION     KHUT01012202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgca ggcggccctg
       61 taagaccggt gtgaaatccc cgggctcaac ctgggaattg cgctggtgac tgcagggcta
      121 gagtacggta gaggggggtg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacaccgat ggcgaaggca gccccctggg cctgtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012203
VERSION     KHUT01012203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcttat
       61 caagtcaatt gtgaaatctc cgggcttaac ccggaacggt caattgatac tgatatgcta
      121 gagtgcagaa agggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacaccaga ggcgaaggcg agttgctagg ctgacactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012204
VERSION     KHUT01012204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           90 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtacgta ggcggtttgg
       61 atagtccgtg gtgaaatctc ccggcttaac cgggaggggt ctgtggatac ttccagactt
      121 gagtgtcgta gaagaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gtcttctagg cgataactga cgctgaggta cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012205
VERSION     KHUT01012205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          100 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtcggtt gtgaaagccc acggcttaac cgtgggtctg cagtcgatac gggcaggcta
      121 gagtgtggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012206
VERSION     KHUT01012206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          100 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgtcg
       61 caagtcaacg gtgaaagctc gatgctcaac atcgagtcgg ccgtcgatac tgcgaggctg
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012207
VERSION     KHUT01012207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           84 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgtg
       61 taagttagat gtgaaatccc taggcttaac ctgggaactg cgtttaatac tgtataacta
      121 gagtactgca gaggttagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actaactggg cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012208
VERSION     KHUT01012208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggattta ctgggcgtaa agcgtgtgta ggcggtccgt
       61 taagtcggat gtgaaagccc tgggctcaac ctaggaattg cattcgatac tggcgggcta
      121 gagtacggta gagggcggcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccgat ggcgaaggca accgcctggg cctgtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012209
VERSION     KHUT01012209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          103 g           39 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agagcgcgca ggcggctcga
       61 tgcgtcccgt gtgaaatccc ccggctcaac tgggggcggc cacgggagac ggtcgggctg
      121 gagcgaggga gagggtcgtg gaattcccgg agtagtggtg aaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg acgacctgga cctctgctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01012210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012210
VERSION     KHUT01012210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          102 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcttgg
       61 gaagtccatg gtgaaagccc gcggctcaac tgcgggtcgg ccaaggatac tcctgagctc
      121 gagtcgacta gaggctaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttagctggg gtcggactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012211
VERSION     KHUT01012211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           94 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtctggt gtgaaatccc ggggcttaac cctggaactg cactggatac tgctttgctt
      121 gagcactgga gagggaactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccggt ggcgaaggcg agttcctgga cagttgctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012212
VERSION     KHUT01012212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           91 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcacgta ggtgggatat
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tggtattcta
      121 gagtttgaca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcggcctggg tcaaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012213
VERSION     KHUT01012213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          100 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcataa agggcgcgta ggcgggtcac
       61 cgagtccgtg gtgaaagccc ccggctcaac cggggaagtg cttcggatac tggtggtctc
      121 gagtggggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccccaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012214
VERSION     KHUT01012214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           82 g           60 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttttt
       61 taagttagtg ttcaaatacg atggctcaac caccggtcgg gcattaaaac tgaaaaactt
      121 gagtaaggtt aaggcagctg gaatttgtgg tgtagcggtg aaatgctcag ataccacaag
      181 gaacgccgat cgtgaagaca ggctgcaggg cctttactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012215
VERSION     KHUT01012215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggagga
       61 taagtcgggc gtgaaatccc tgggctcaac ccaggaactg cactcgaaac tgcccttctt
      121 gagtaccgga gaggaaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012216
VERSION     KHUT01012216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           82 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggctggatat
       61 taagtaggaa gtgaaaggtt ggagctcaac tctgacactg ctccctatac tggtattctt
      121 gagtcttgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccgat ggcgaaagca gctttctgga caagtactga cgctgaggtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01012217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012217
VERSION     KHUT01012217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           87 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgtgcgta ggcggttgac
       61 taagtctggt gtgaaatccc tgaacccaat tcaggacgtg cattggatac tggtcgacta
      121 gagtatggga gaggaaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctttctgga ccattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012218
VERSION     KHUT01012218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           85 g           57 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggctttg
       61 taagtcaacg attaaagatc gacgcttaac gtcgggaatg ttgttgatac tgcatggctt
      121 gaggataaca ggggcaggcg gaactttagg tgtaggggtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttgctagg ttatttctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012219
VERSION     KHUT01012219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           94 g           40 t
ORIGIN      
        1 tacagaggag gccagcgttg tccggactta ctgggcgtaa agggtccgca ggtggtcggg
       61 caagcagggt gtgaaatccg ccggctcaac tggcggtcgc catcctgaac tgcacgactt
      121 gagggctgga gaggtcggtg gaattgccgg agtagtagtg atatgcgtag ataccggcag
      181 gaacgcccaa ggggaagcca accgactggt cagcacctga cactcaggga cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01012220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012220
VERSION     KHUT01012220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggccttt
       61 caagttggat gtgaaagccc tgggctcaac ccaggaagtg cattcaaaac tgtgaggctt
      121 gaatcccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cgggtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012221
VERSION     KHUT01012221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          102 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcacca
       61 caagtacgca ctgaaatctc cctgctcaac ggggagggag gtgggtagac tgtggagcta
      121 gaggacggta gagggtggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gccacctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012222
VERSION     KHUT01012222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           86 g           53 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ctgggcgtaa agagctcgta ggcggtttgc
       61 caagtcgaaa gtcaaaacta caggctcaac ttgtagtcgc tttcgatact ggtatgactt
      121 gaatctggta ggggatcacg gaattcctgg tgtagcggtg aaatgcgcag agatcaggag
      181 gaacaccaat ggcgaaggca gtgatctggg ccagtattga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012223
VERSION     KHUT01012223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           78 g           57 t
ORIGIN      
        1 tacgagtacc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggtctta
       61 ttagtctttt gttaaaattt ccggcttaac cggagacatg cagaggaaac ggtaagacta
      121 gagtgtgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcattactga cactgaaaca cgaaagcgtg
      241 ggtagcgaac tgg
//
LOCUS       KHUT01012224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012224
VERSION     KHUT01012224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           85 g           53 t
ORIGIN      
        1 gacgaaccat gcaaacgtta ttcggattca ctgggcttaa agcgtgcgta ggcggctttg
       61 ttagtcgggt gtgaaagccc tcagctcaac tgaggaattg cgctcgaaac tgcaaggcta
      121 gagaatgata gaggtgggtg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaggcg acccactggg tcatttctga cgctgaggta cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012225
VERSION     KHUT01012225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           86 g           52 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggttgtg
       61 ttagtctcat gtcaaattct tcggcttaac cggggtaccg catgggaaac ggcacaacta
      121 gaggatgcga ggggtgagtg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca gctcactggc gcactcctga cgctgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01012226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012226
VERSION     KHUT01012226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           85 g           51 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggcagtg
       61 ttagtcggtt gttaaaaccc ggggcttaac cccggacatg cgatcgaaac ggcacagcta
      121 gaggatgcga gggatgtatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtacattggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01012227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012227
VERSION     KHUT01012227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggtgtgta ggtggctggg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaatgg cctttgatac tgccgagcta
      121 gagttttgta gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gccgcctgga caaaaactga cactgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012228
VERSION     KHUT01012228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctct
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgtaac tgagaggctt
      121 gagtgcggca gaggggggtg gaattccagg tgtagcagtg aaatgcgtag atatctggag
      181 gaacaccgat ggcgaaggca gccccctggg tcgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012229
VERSION     KHUT01012229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           98 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttaa
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg cattgcaaac gattgaactg
      121 gagtcgtgga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc cacagactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012230
VERSION     KHUT01012230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtttag
       61 taagcggagg gtgaaatccc agggctcaac tctggggctg ctctccgaac tgctggacta
      121 gagtacggga gaggacagtg gaattcctgg tgtagcggtg gaatgcgtaa atatcaggag
      181 gaacacccgt ggcgaaggct gctgtctgga acgttactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012231
VERSION     KHUT01012231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c           96 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ttgggcttaa agggcgcgta ggcgggcttt
       61 caagtccggg gtgaaatcct ccagctcaac tggagaacgg ccttggatac tggaggtctt
      121 gagggaggta ggggcatctg gaactgtggg tggagcggtg aaatgcgttg atattcacag
      181 gaactccggt ggcgaaggcg aggtgctgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012232
VERSION     KHUT01012232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctctt
       61 taagtgactg gtgaaatccc cgggctcaac tcgggagggt ctggtcatac tggggagctg
      121 gagtgtggga gaggtgagtg gaactccagg tgtagcggtg atatgcgtag agatctggaa
      181 gaacacccgt ggcgaaggcg gctcactgga ccacaactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012233
VERSION     KHUT01012233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 tacgaaggtt gcaagcgttg ttcggaatca ctgggcttaa agcgcatgta ggtggaccag
       61 aaagcacttt gtgaaatccc ccggcttaac cggggaatcg cttggtgaac tactggtctt
      121 gaggcaagta ggggtgcatg gaactcttgg tggagcggtg gaatgcgtag atatcaagag
      181 gaacgccaga ggtgaaaacg gtgcactggg cttgttctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012234
VERSION     KHUT01012234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           96 g           48 t
ORIGIN      
        1 aacgtagggg gcaagcgtta ttcggattta ctgggcgtaa agcgcgtgca ggcggctctg
       61 caagtcggac gttaaatctc ctggctcaac tgggaggggt cgttcgatac tgtggggctt
      121 gaggttggga gagggaagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcttcctggc ccacacctga cgctaagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012235
VERSION     KHUT01012235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattca ctgggcgtaa agcgtgtgca ggcggttgga
       61 taagttggat gtgaaatctc ctggctcaac tgggagaggc cgttcgatac tgttcgacta
      121 gagggcaaca gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctggg ttgcatctga cgctcagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012236
VERSION     KHUT01012236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 taagtcaaat gtgaaagccc tgagcttaac tcaggaattg catatgatac tgctagactc
      121 gagtgtcgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012237
VERSION     KHUT01012237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctac
       61 taagtcgaac gtgaaagccc caggcttaac ctgggaactg cgtttgatac tggaaggctt
      121 ggattcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacatcga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012238
VERSION     KHUT01012238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggttttt
       61 taagtcagat gtgaaattcc ggggctcaac ctcggaattg catttgaaac tggagagcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012239
VERSION     KHUT01012239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctatt
       61 caagtcagag gtgaaagccc ggggctcaac cccggaattg cctttgaaac tagatagcta
      121 gaatcacgga gaggtaagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcttactgga cgtgaattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012240
VERSION     KHUT01012240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           88 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ctgggcgtaa agagagcgta ggcgggatcg
       61 taagttagga gtttaatcca cgggctcaac ctgtggtctg cttttaatac tgcggttctt
      121 gagtatggga gagggcgatg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt agcgaaggcg gtcgcctggc ccaatactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012241
VERSION     KHUT01012241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           87 g           55 t
ORIGIN      
        1 tacgaaggtt gcaagcgttg ttcggaatca ctgggcttaa agcgcatgta ggcggacagg
       61 aaagcgtttt gtgaaatccc tcggcttaac cgaggaattg cttggcgaac ttcctgtctt
      121 gaggcaagta ggggtgcatg gaactcttgg tggagcggtg gaatgcgtag atatcaagag
      181 gaacgccaat ggtgaaaaca gtgcactggg cttgtactga cgctgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012242
VERSION     KHUT01012242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           89 g           52 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttat
       61 taagttagtt gtgaaagccc tgggctcaac ctaggaagta caactaagac tgatagacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctatctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012243
VERSION     KHUT01012243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           93 g           47 t
ORIGIN      
        1 gacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtttat
       61 caagtttgat gtgaaagtcc tgggcttaac cgagggaggg cgtcaaagac tgataagcta
      121 gagtaagaga gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccaga ggcgaaggcg acgacctggc ttttaactga cactgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012244
VERSION     KHUT01012244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           94 g           46 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggctgtg
       61 ttagtcgggc atgaaatctc tcggctcaac cgggagaggc tgtccgatac tgcgcagctt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgcccctga cgctgaaacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012245
VERSION     KHUT01012245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccgtt
       61 ttagtccgac gtgaaatccc acggctcaac cgtggaactg cgtcggatac tggacggctt
      121 gaatccaaga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ttggtattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012246
VERSION     KHUT01012246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           45 t
ORIGIN      
        1 tacgtagggc gcaagcgtta ctcggaatca ctgggcataa agggcgtgta ggcggagacg
       61 taagtccggt gtaaaatccc ccggctcaac cggggaatgc cattggatac tgcgtctctt
      121 gagtgcggga ggggagagtg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccggt ggcgaaagcg actctctggc ccgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012247
VERSION     KHUT01012247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           81 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtgggtttg
       61 taagtacctt gtgaaatccc atggctcaac catggaactg ctgggtatac tgcgaatctt
      121 gagctgtcta gaggttactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtaactggg gacacgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012248
VERSION     KHUT01012248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctacc
       61 taagtctgat gtgaaatccc ggggcttacc cccggaagtg cattggaaac tgggtagcta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012249
VERSION     KHUT01012249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           90 g           47 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccgaattta ctgggcgtaa agcgcgtgca ggcggtccaa
       61 caagttggat gtgaaatctc ctggctcaac tgggagaggt cgttcaaaac tgttggactg
      121 gaggacggta gagggaggtg gaattcccgg tgtagtagtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcctcctgga ccgtacctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012250
VERSION     KHUT01012250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcaac
       61 caagtcaggg gtgaaatctt tcagcttaac tggaaaacag cctctgatac tggttgtctg
      121 gagggaggta ggggcctgtg gaactgctgg tggagcggtg aaatgcgtag atatcagcag
      181 gaacgccggt ggcgaaagcg acgggctgga cctcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012251
VERSION     KHUT01012251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacgaaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcggtcttg
       61 caagtcggat gtgaaagccc tgggcttaac ctgggaactg catccgaaac tgcttgactt
      121 gaatgcttga gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcgga ggcgaaggcg gccaactggt gatgcattga cgctaaggtg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01012252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012252
VERSION     KHUT01012252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           43 t
ORIGIN      
        1 gacggagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggctctt
       61 taagtcggat gtgaaatccc ccggctcaac cggggaactg catccgatac tggggagctc
      121 gagaatggtt ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccatcactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012253
VERSION     KHUT01012253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           40 c           93 g           53 t
ORIGIN      
        1 tacggggggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtttac
       61 caagtcaggt gtgaaagcct tgggcttaac ctgagaatag catttggtac tggtagacta
      121 gaggacagga gaggaaagcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccgga ggcgaaggcg gctttctgga ctgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012254
VERSION     KHUT01012254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           85 g           55 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatga ctgggcgtaa agggtacgta ggcggcttta
       61 taagtaggat gtgaaatccc cgggcttaac ccgggaactg cattctatac tgtaaggcta
      121 gagatcgata gaggaaacag gaattcctag tgtaggggta atatccatag atattaggag
      181 gaacaccagt ggcgaaggcg agtttctggg tcgatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012255             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012255
VERSION     KHUT01012255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           89 g           49 t
ORIGIN      
        1 tacgtagggt ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagtcggat gtgaaaactc ggggctcaac cccgagacgc catccgatac tgctgtggct
      121 tgagtttggt aggggagcac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtgctctgg gccaacactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012256             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012256
VERSION     KHUT01012256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           99 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctgac
       61 cgagtcgggc gtgaaattct caggctcaac ctgagatggt cgttcgatac tggtctggct
      121 ggagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggtctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012257
VERSION     KHUT01012257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           86 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcgggta ggcggttggg
       61 taagtctgtt gtgaaagctc ctggcttaac tgggagaggt caacagaaac tacccagctc
      121 gagtgtggta gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggcttctgga ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012258
VERSION     KHUT01012258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           62 c          100 g           39 t
ORIGIN      
        1 gacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggctcgc
       61 caagcccgaa gtgaaatgcc ccggcctaac cggggacgtg cttcggggac tggcgggctc
      121 gggcgagcta ggggtctgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acagactggg gctttgccga cgctcatgca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012259
VERSION     KHUT01012259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           92 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttgt
       61 caagtcaggc gtgaaagccc cgggcttaac ctgggaggtg cgtttgatac tggcaagcta
      121 gagagtgaga gaggaaagca gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaataccaga ggcgaaggcg gctttctggc tcactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012260
VERSION     KHUT01012260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           52 c          106 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatca ctgggtgtaa agcgcgtgta ggcggcctgc
       61 caagtctgtc gtgaaagggc ccggcttaac cgggtcaggt cgatggaaac tggtgggctg
      121 gaggccggta gaggtgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcactggg ccgagcctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012261
VERSION     KHUT01012261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           95 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccgtg
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaaggg cgcccgaaac tgcatggctt
      121 gagtaccgga gaggatggcg gaattctcca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccatctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012262
VERSION     KHUT01012262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           86 g           56 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agagtctgta ggcggctctg
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg catctgatac tgcttagctt
      121 gaatgttgga gaggaaagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctttctggc caaacattga cgctgagaga cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012263
VERSION     KHUT01012263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agagcgtgta ggcggtctag
       61 caagtcagat gtgaaagccc agagcctaac tctggaagtg catttgaaac tgctaggctt
      121 gagtacagta gaggttggcg gaatacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccaactggg ctgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012264             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012264
VERSION     KHUT01012264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          107 g           36 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtcttg
       61 tgcgcgtgcc gtgaaagcct ggggctcaac cccaggtcgg cggtgcgaac ggcaggactg
      121 gagcatgcga gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggg ggcgaaggcg gcctgctggc gcagtagctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012265             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012265
VERSION     KHUT01012265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           94 g           52 t
ORIGIN      
        1 tacggagggg gcaagcgtta ttcggattta ctgggcgtaa agggtgtgta ggcggtcgcg
       61 caagtctgat gtgaaatctt caccacttaa cgggaagagg tcattggaaa ctgcgtgact
      121 tgagtttggt agaggcgagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacaccgg tggcgaaggc ggctcgctgg gccaatactg acgctgagac acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012266
VERSION     KHUT01012266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgtc
       61 taagtatctt gtgaaatccc acagctcaac tgtggaactg ctggatatac tggacgtctt
      121 gaggtattca ggggcgaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgcctgt ggtgaagacg ggtcgctggg aatattctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012267             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012267
VERSION     KHUT01012267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          108 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggtggcgccg
       61 taggttcgtc gttaaagccc gaggctcaac ttcgggtggt cggcggagac cgcggggctt
      121 ggaggccggt agagggatgc ggaattcccg gtgtagtggt gaaatgcgta gagatcggga
      181 ggaacaccag aggcgaaagc ggcatcctgg gccggacctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01012268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012268
VERSION     KHUT01012268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacggagagt gcgagcgttg ttcggaatta ctgggcgtaa agggcgtcta ggcggcttgc
       61 caagtcagat gtgaaatccc tcggctcaac cgaggacgtg catctgaaac tggtgagctt
      121 gagtattgga gggggcagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggc caaatactga cgctgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012269
VERSION     KHUT01012269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           92 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttggg
       61 aaagtcttct gtgaaatcta cgggctcagc ctgtaacgtg cagaggaaac tactcggctt
      121 gagtgtcgga gaggataggg gaatacccgg tgtagaggtg aaattcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg cctgtctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012270
VERSION     KHUT01012270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          108 g           37 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggatgct
       61 cgcatcggtg gtgaaagccc cccgctcaac gggggagggt ccaccgagat ggggtatctg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctggctgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012271
VERSION     KHUT01012271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           95 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtgggg
       61 taagtctgat gtgaaaggcg ggggctcaac ctccgtatgg cattggatac tgctctacta
      121 gagtcctgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga caggaactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012272
VERSION     KHUT01012272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c          100 g           35 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggcctga
       61 aaagtctgat gtgaaagcgc ccggctcaac cgggcgaggc cattggaaac ttttaggctg
      121 gagggcagta gaggcaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctgctggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012273
VERSION     KHUT01012273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcattt
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tggaacgcta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012274
VERSION     KHUT01012274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcagac gtgaaatccc tcggcttaac cggggaacgg catctgatac tggatggctg
      121 gagttcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacactga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012275
VERSION     KHUT01012275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           91 g           53 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtagag
       61 taagtctgat gtgaaatccc agggctcaac cctggaactg cattggatac tgctttgctt
      121 gagtactgga gggggaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaaggccagt ggcgaaggcg agttcctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012276
VERSION     KHUT01012276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           47 c           98 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttagt
       61 cacgtcggct gtgaaatctc ctggcttaac tgggagtttg ctgtcgatac gggctggctt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012277
VERSION     KHUT01012277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccgcg
       61 caggtcggat gtgaaagccc ttggctcaac caaggaagtg cattcgaaac cgcacggctc
      121 gagtcccgga gaggaaggcg gaattcttgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gccttctgga cgaggactga cgctgagatg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012278
VERSION     KHUT01012278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           78 g           53 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtgcgca ggcggattta
       61 caagtcagaa gtgaaatccc taggctcaac ctaggaattg cttttgaaac tgtagatcta
      121 gaatcccgga gaggttaaca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgtaagcg gttaactgga cgggcattga cgctcaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012279
VERSION     KHUT01012279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatact
       61 taagtcagag gtgaaatccc agggcccaac tctggaactg cctttgatac tgggtatctt
      121 gaggatggca gaggcaagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg acttgctggt ccattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012280
VERSION     KHUT01012280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c          103 g           36 t
ORIGIN      
        1 tacagggggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtcttg
       61 gacgtgccgt gtgacatccg tcggctcaac cgacggaggc cagggtacac gacaagactg
      121 gaggcagaga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcatcctggc tctgtactga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012281
VERSION     KHUT01012281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           95 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggtttt
       61 caagtcaggg gtgaaatgcc ggggcttaac ctcggaactg cctttgatac tgggagcctg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012282
VERSION     KHUT01012282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           81 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggactat
       61 taagtaagaa gtgaaaggtt ggagctcaac tccgacactg ctccttatac tggtagtctt
      121 gagtcttgga gaggaaagcg gaacgacaca tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccgat ggcgaaagca gctttctgga caagtactga cgctgaggtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01012283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012283
VERSION     KHUT01012283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcgat
       61 aaagtagggt gtgaaatccc ccggctcaac cggggaatgg cgttctaaac taatcgactg
      121 gagtaagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctatactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012284
VERSION     KHUT01012284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c          108 g           33 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggcggag
       61 tatgtgccgt gtgacaggcg ggagctcaac tcccgccggc cagggtagac agctcggctg
      121 gaggcagtga gagggaggtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgcaggcg acctcctggc actgacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012285
VERSION     KHUT01012285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           88 g           55 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggtggtttaa
       61 taagtcaggt gtgaaagccc ggggctcaac tccgggattg catttgaaac tattagacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012286
VERSION     KHUT01012286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggatatg
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaatgg catacgatac tgtatatctc
      121 gagttggtta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcttcctggc accaaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012287
VERSION     KHUT01012287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          102 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttttt
       61 taagttcgtg gtgaaagacc ggggctcaac cccggagcgt ccgcgaagac tggaagactg
      121 gagtgtcgga gaggagtgcg gaatccctgg tgtagcggtg gaatgcgtag agatcagggg
      181 gaacaccgat ggcgaaggca gcactctggc cgaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012288
VERSION     KHUT01012288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           62 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaccgg
       61 cgagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctctgatac cgccggcctt
      121 gagggggata ggggcgtgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacaccggt ggcgaaggcg gcacgctggg tccctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012289
VERSION     KHUT01012289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttcg
       61 caagtctgct gtgaaagccc cgggcttaac ctgggaatgg cagtggaaac tatgggactt
      121 gaatctggca gaggggggtg gaactccacg tgtagcagtg aaatgcgtag atatgtggaa
      181 gaacaccgat ggcgaaggca gccccctggg ctgagattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012290
VERSION     KHUT01012290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggcggctaga
       61 taagtcagat gtgaaagccc ggggctcaac cccgggactg catcagaaac tatttagcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012291
VERSION     KHUT01012291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctgcg gtgaaagccc agggcttaac cctggaagtg ccgcagaaac ggtgagactg
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caggtactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012292
VERSION     KHUT01012292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctag
       61 ccagtccggg gtgaaagcct ccagctcaac tggagaagtg ccttggatac tgctggactc
      121 gagcgaggta ggggtgatgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg catcactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012293
VERSION     KHUT01012293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           81 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggctga
       61 taagtcagtg gtgaaagctc cgagcttaac ttggaaactg ccattgatac tgccagtctt
      121 gaatgtcgta gaggtaagcg gaatgttgca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccaat tgcgaaggca gcttactatg cgatgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012294
VERSION     KHUT01012294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtctgtt gtgaaatccc cgggcttaac ctgggaatgg caatggagac tgcaaggcta
      121 gagtttggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaagactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012295
VERSION     KHUT01012295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggttacg
       61 tatgtctgaa gtgaaatccc tgagcttaac tcaggacgtg ctttggaaac ggcgtaacta
      121 gagtactgga gaggttcgta gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaataccagc ggcgaaggcg gcgaactgga cagttactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012296
VERSION     KHUT01012296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           92 g           53 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggctttt
       61 taagttggat gtgaaatacc cgagcttaac ttggggggtg cattcaaaac tggagagcta
      121 gagttcagga gagggaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgacactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012297
VERSION     KHUT01012297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcgggtctg
       61 taagtcgatt gtgaaaaccc tgggcttaac ccgggacgtg cagtcgaaac tgcagatctt
      121 gagtgcctga gaggatggcg gaattcccgg tgtagtagtg aaatacgtag atatcgggaa
      181 gaacacctga ggcgaaggcg gctatctggc ggtgcactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012298
VERSION     KHUT01012298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggtttaa
       61 tgtgaggtat gtgaaagccc cgagcttaac ttgggaagtg catagctaac tattgaacta
      121 gagtatatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cactgaagcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012299
VERSION     KHUT01012299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           88 g           54 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctata
       61 taagtctaat gtgaaatacc ttggcttaac caaggaagtg cattggatac tgtatagctt
      121 gagtcccaaa gagggcgatg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcagt ggcgaaggcg atcgcctggt tggagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012300             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012300
VERSION     KHUT01012300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c          100 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcctgt
       61 cgcgtcagcg gtgaaaggtg tgggctcaac ccgcacattg ctgctgatac gggcaggctc
      121 gaggctggaa gaggagagtg gaatgccctg tgtagcggtg aaatgcgcag atatagggca
      181 gaacacccgt ggcgaaggcg gctctctggg acagttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012301
VERSION     KHUT01012301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           82 g           54 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcaa
       61 caagtcgggt gtgaaatcct ccagctcaac tggagaacag cgcccgaaac tgttgtgctt
      121 gaggaataca gaggtatgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcatactggg tattttctga cgctcatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012302             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012302
VERSION     KHUT01012302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtcgtc
       61 tgtgtcgggc gtgaaatccc aaggctcaac cttggaatgg cgctcgaaac tgggcgactt
      121 gagtgcagga tgggagagcg gaattcttgg tgtagcggtg aaatgcgttg atatcaagaa
      181 gaacaccggt ggcgaaggcg gctctctgga atgctactga cactgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012303
VERSION     KHUT01012303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggcgcgt
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgaaac tggcgtgctt
      121 gaatatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcgacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012304
VERSION     KHUT01012304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 gacgagctct ccaaacgtta ttcggtatca ctgggcttaa agagttcgta ggcggcttga
       61 caggtgaggt gtgaaagccc acggcctaac cgtggaattg cgcttcaaac cgtcgggcta
      121 gagggatgca ggggtatgcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacaccggt ggcgaaagcg gcatactggg cattttctga cgctgaggaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012305
VERSION     KHUT01012305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtaaac
       61 catgtcaggg gtgaaatccc ggggctcaac tccggaattg cctttgaaac gggtttgcta
      121 gagtccgaga gaggatggcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggc tcggtactga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012306
VERSION     KHUT01012306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           90 g           50 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggaccaa
       61 tgtgtcagat gtgaaagccc tgggctcaac ctaggaaatg catttgaaac tgttggtctt
      121 gagtgttgga gagggtcgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgacctgga caataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012307
VERSION     KHUT01012307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           85 g           59 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agggtgcgta ggttgccatt
       61 caagtcagtt gtgaaatccc cgggctcaac ccgggaagtg cttctgatac tgtttggctt
      121 gagtactgga gaggatagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctatctggc catgtactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012308
VERSION     KHUT01012308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           85 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagtgcgta ggtggataat
       61 taagtcatat gtgaaatccc cgggcttaac ctgggaattg cgtctgaaac tggttgtcta
      121 gagtatggta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actgtctggg ccaatactga cactgaggta cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012309
VERSION     KHUT01012309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 gacgaaggtg gcgagcgttg ttcggaatca ctgggcataa agcgcacgta ggcggcgacg
       61 taagttgggt gtgaaatccc tcggcccaac cgaggaactg cgcccagtac tgcgttgctt
      121 gaggatcaaa ggggtgcatg gaattcccgg tggagcggtg aaatgcgttg atatcgggag
      181 gaactccggc ggcgaaagcg gtgcactggt tgattactga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012310
VERSION     KHUT01012310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           90 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgtta atcggaatga ttgggcgtaa agggcgcgta ggtggtaatc
       61 caggtttggt gtgaaagctc tcggctcaac cgagaaaggg catcaaatac cagattgcta
      121 gagtgagaga gaggactgtg gaactttcgg tgtagcggtg aaatgcgtag atatcgaaag
      181 gaacaccaga ggcgaaggcg acagtctggc tctatactga cactgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012311
VERSION     KHUT01012311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtgggttga
       61 taagtcagtg gtgaaatccc caagcttaac ttgggaactg ccattgatac tattaatctt
      121 gaatactgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat agcgaaggca gcttactacg cagttattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012312
VERSION     KHUT01012312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           81 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcagg
       61 taagtttgaa gtgaaagctc catgctcaac ttggaaaatg cttcatatac tatctgacta
      121 gagagccgga gaggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg agtctctgga cggcatctga cgctgaggca cgaaagccaa
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012313
VERSION     KHUT01012313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           84 g           54 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggcaaat
       61 caagtctgtt gttaaatttc tcggcccaac cgggagcatg cagcggatac tgatttgata
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012314
VERSION     KHUT01012314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           83 g           55 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtttcgt
       61 taagtctcta gtgaaagccc tcggctcaac tgaggaactg ttagagatac tggcggaata
      121 gagtatggca gaggcagacg gaattcttgg tgtagcggta aaatgcgttg atatcaagaa
      181 gaacaccagt ggcgaaggcg gtctgctggg ccagtactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012315
VERSION     KHUT01012315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           94 g           48 t
ORIGIN      
        1 gacagaggtt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtgaag
       61 tgtgtctgat gtgaaagccc tcggcttaac cggggaagtg catcggaaac tgctttgcta
      121 gagtgcgaga gaggatggcg gaattcccag agtagaggtg aaattcgtag atactgggag
      181 gaacacctga ggcgaaggcg gccatctggt tcgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012316
VERSION     KHUT01012316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           78 g           58 t
ORIGIN      
        1 tacgaaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttac
       61 taagtcagtg gtgaaagccc ggagctcaac tccggaattg ccattgatac tgatgagctt
      121 gagtacactt gaggtaggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gcttactaaa gtgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012317
VERSION     KHUT01012317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggccgct
       61 caagtcgggg gtgaaatcgt tccgcttaac ggaacaagtg ccttcgatac tgggtggctg
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012318
VERSION     KHUT01012318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           99 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgacc
       61 caagtcaacg gtgaaagctc gacgctcaac gtcgagtcgg ccgtcgatac tgggttgctc
      121 gagcactgta gaggctcatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgagctggg cagtagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012319
VERSION     KHUT01012319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           99 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggagact
       61 taagtctggt gggaaagccc ctggctcaac cagggaagga cgctggaaac tgggtctctt
      121 gagggcgaca gagggaagtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctggg tcgcccctga cactgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012320
VERSION     KHUT01012320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           95 g           40 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgcgtgta ggcggtccgt
       61 caagtcgggt gtgaaatctc gaggctcaac ctcgaaatcg catccgatac tggcggactt
      121 gaggacggta gaggtcggtg gaattcccgg tgtaccggtg aaatgggcag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacg cgaaagccag
      241 gggagcgaac cgg
//
LOCUS       KHUT01012321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012321
VERSION     KHUT01012321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c          101 g           35 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggccgcg
       61 cccgtccggt gtgaaagccc ggggctcaac cccggaaggg cactggagac ggcgcgactg
      121 gagtgctgaa gaggagggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccctctggg cagcgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012322
VERSION     KHUT01012322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           90 g           46 t
ORIGIN      
        1 gacagaggat gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctttg
       61 aaagtcgggt gtgaaagccc caggctcaac ctgggaagtg cactcgatac tgcaaagcta
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012323
VERSION     KHUT01012323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtca
       61 caagtctgtg gtgaaatccc ccggctcaac cggggaactg ccgtggatac tgtgactctc
      121 gaggggtgta ggggcatgcg gaacttcagg tggagcggtg aaatgcgtag atatctgaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012324
VERSION     KHUT01012324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           60 c          110 g           39 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccgtt
       61 caagtctggc gtgaaagccc ccggctcaac cggggagggt cgtcggagac tgggcggctt
      121 gagggcggta ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg ccgtccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012325
VERSION     KHUT01012325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           87 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggatatt
       61 taagtcgaat gtgaaatccc tgggcttaac ctgggaactg catccgatac tgggtatctg
      121 gagtatggta gaggaaagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg actttctgga tcaatactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012326
VERSION     KHUT01012326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           92 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtgtca
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tgtgatgctc
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gcttactgga ccacgactga cgctgagaca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012327
VERSION     KHUT01012327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           92 g           45 t
ORIGIN      
        1 tacagggggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggatcag
       61 gaagtgtttc gtgaaaaccc ccggctcaac cggggaattg cggggcaaac tcctgatctt
      121 gagggaagta tgggcggaca gaactatcgg tggagcggtg aaatgcgtag atatcgatag
      181 gaatgccgat ggcgaaggcg gtccgctggg cttctcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012328
VERSION     KHUT01012328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           58 c           93 g           38 t
ORIGIN      
        1 tacagaggcc acaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggactcg
       61 aaagcgtcct gtgaaagccc ccggctcaac cggggaacag cagggcgaac tgcgagtcta
      121 gaagcaagta ggcgcgggca gaacagtggg tggagcggtg aaatgcgtag atatccactg
      181 gaatgccgat ggggaagccg gcccgctggg cttgtcttga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012329
VERSION     KHUT01012329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctctt
       61 caagtcagcc gtgaaatctc caggcttaac ctggaagggt cggttgatac tgtcgagcta
      121 gagtgtagaa gaggcaactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacacctgc ggcgaaggcg ggttgctggg ctaacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012330
VERSION     KHUT01012330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctatg
       61 caagtcagag gtgaaatacc cgggctcaac ccgggaactg cctttgaaac tgcatagctg
      121 gagtgtcgga ggggatagcg gaatttctaa tgtagcggtg aaatgcgtag atattagaag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012331
VERSION     KHUT01012331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c          100 g           42 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggttatg
       61 taagtggtgg gtgaaagccc cgggcttaac ctgggaagtg cccatcaaac tgcatgacta
      121 gagtacagga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaggcg actgcctggc ctgaaactga cgctgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012332
VERSION     KHUT01012332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           91 g           55 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggagcg
       61 taagtctgga aggaaatctc aaggctcaac tttggggctt tttcagatac tgcgtttctt
      121 gagggtatca gaggatagta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg actgtctggg atattcctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012333
VERSION     KHUT01012333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           91 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggtccac
       61 caagtcaggg gtgaaatctt tcagcttaac tggaaaagtg cctttgatac tggtggactc
      121 gagcgaggta ggggcagatg gaaccgtggg tggagcggtg aaatgtgtag atatccatgg
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012334
VERSION     KHUT01012334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           85 g           53 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtcattt gctaaagcct ggggctcaac cccagttctg cggatgaaac ggcgcgacta
      121 gaggatgcga gggatgtatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtacattggc gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01012335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012335
VERSION     KHUT01012335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtaagg
       61 caagtctgat gtgaaatctc caagctcaac ttggaaatgg cattggaaac tgctttactt
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacatctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012336
VERSION     KHUT01012336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgca ggcggttagt
       61 taagtcgggt gtgaaatccc tgggctcaac cgaggaagtg catccgaaac taactagcta
      121 gagaatggta gaggaaggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaataccggt ggcgaaggcg gccttctgga ccatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012337
VERSION     KHUT01012337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           83 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgtta ttcggaatta ctgggcgtac agggtgcgta gacggcttat
       61 taagtcggtt gttaaagatt ccggcctaac cggaaaaatg cggtcgatac tgataagcta
      121 gaggttggaa gagagaagtg gaattctcgg agtagcggtc aaatgcgtag atatcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatacctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012338
VERSION     KHUT01012338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           94 g           47 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtgaag
       61 tgtgtctgac gtgaaagccc tcggcttaac cggggaaatg cattggaaac tacttcgctg
      121 gagtgcgaga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccgga ggcgaaggcg gccatctgga tcgtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012339
VERSION     KHUT01012339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           92 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaataa ctgggcgtaa agggtgcgta ggcggtaaag
       61 cgagcttgat gtgaaatccc cgggcttaac ctgggaatgg cattgaggac agtttaacta
      121 gagtctgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataagactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012340
VERSION     KHUT01012340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           86 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagtgca ggcggctcga
       61 taagtctgat gtgaaagcct tcggctcaac cggagaattg catcagaaac tgtcgagctt
      121 gagtacagaa gaggagagtg gaactccatg tgtagcggtg aaatgcgtag atatatggaa
      181 gaacaccggt ggcgaaggcg gctctctggt ctgttactga cgctgaggct cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012341
VERSION     KHUT01012341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           81 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtat agggcgcgta ggcggacttt
       61 caagtcggat gtgaaatcct agggctcaac cttagaacag catccgaaac tggtagtcta
      121 gagggtagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01012342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012342
VERSION     KHUT01012342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           82 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca gacggtcaac
       61 taagtcaact gttaaatttc tcagcctaac tgggagctcg cagtagaaac tggtcgacta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012343
VERSION     KHUT01012343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           91 g           42 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggccacg
       61 caggtcggat gtgaaatccc tcggctcaac caaggaagtg cattcgaaac cgcatggctc
      121 gagtcccgga gagggtggtg gaattctagg cgtagaggtg aaattcgtag atttctggag
      181 gaacaccggc ggcgaaagcg gccacctgga cgaggactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012344
VERSION     KHUT01012344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ctgggcgtaa agagttcgta ggcggtttgt
       61 taagtttaat gtgaaagccc tgggctcaat ccaggaagtg cattagatac tggcaaactg
      121 gagtgcagga gaggagagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctctctgga ctgtcactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012345
VERSION     KHUT01012345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           86 g           51 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggtggtctgt
       61 tatgtcggat gtgaaagccc tcggcttaac tgaggaagtg catccgaaac tggcagactt
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaat ggcgaagacg gcgacctgat tcgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012346
VERSION     KHUT01012346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgggggag caagcgttgt tcggatttac tgggcgtaaa gggcgcgtag gcggccagca
       61 caagtcagtt gtgaaatctc cgagcttaac tcggaaaggt caactgatac tgtgcggcta
      121 gagtgcggaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctgc ggcgaaggcg ggttgctggg ccgacactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012347
VERSION     KHUT01012347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           88 g           50 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagttc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tggttctctt
      121 gggccatcta ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca gctcgctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012348
VERSION     KHUT01012348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           83 g           51 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccgaa
       61 tgtgtcaggt gtgaaagccc gaggcttaac ctcggaattg catttgaaac tattcggcta
      121 gagtattgga gagggtaacg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gttacctgga caattactga cgctcaggca cgaaagcatg
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01012349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012349
VERSION     KHUT01012349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           87 g           50 t
ORIGIN      
        1 tacagagggt gcaagtgttg ttcggaatta ttgggcgtaa agcgcgcgta ggttgtttag
       61 taagtcgaat gtgaaagccc tgggctcaac ccaggaactg catccgaaac tgctaagcta
      121 gagtttggta gaggagagtg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggg ccaaaactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012350
VERSION     KHUT01012350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           85 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggaaaat
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaattg caactgaaac tggttttcta
      121 gagtatggta gaggacagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacaccagt ggcgaaggcg actgtctggg ccaatactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012351
VERSION     KHUT01012351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           82 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcgggtttt
       61 taagtaggaa gtgaaaggtt ggagctcaac tccaacactg ctccctatac tgggaatctt
      121 gagtgccgga gaggaagacg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca gtcttctgga cggatactga cgctgagata cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01012352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012352
VERSION     KHUT01012352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           80 g           53 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggctgtttta
       61 taagttggaa gtgaaagccc agggctcaac cctggaattg ctttcaaaac tataagactc
      121 gaatctcgga gaggatagcg gaatttccag tgtagtagtg aaatacgtag atattggaag
      181 gaacaccagt ggcgcaagcg gctatctgga cgagcattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012353
VERSION     KHUT01012353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           98 g           36 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtcgcg
       61 cgcgtggggt gtgaaagcgc tcggcttaac tgagcgaagc catcccatac ggcgcgactc
      121 gagcccgtga gaggtccatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggc acggagctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012354
VERSION     KHUT01012354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttatt
       61 taagtgagat gtgaaatccc cgggctcaac ctgggaactg catcttatac tgggtaactg
      121 gagtacatta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga atgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012355
VERSION     KHUT01012355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           59 c          109 g           38 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctatc
       61 caagcatggc gtgaaagccc ccggctcaac cggggagggt cgtcgtggac tgggtggctt
      121 gagggcggga ggggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctgga ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012356
VERSION     KHUT01012356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggttgg
       61 taagtatctt gtgaaatccc acggcttaac cgtggaattg ctgggtatac tgccgatctt
      121 gagtcatcta gaggtaaccg gaacagatgg tggagcggtg aaatgcatag atatcatctg
      181 gaacgccaat ggtgcagaca ggttactggg gatgtactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012357
VERSION     KHUT01012357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           81 g           57 t
ORIGIN      
        1 tacgagaggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgcgca gacggtttgt
       61 taagtcaatt gtttaattcc tcggcctaac tgaggatcag cagtagaaac tgataaacta
      121 gagtgtgaga gagggaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcctggc tcattactga cgttcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012358
VERSION     KHUT01012358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtacctt gtgaaatccc ccggctcaac cggggaactg ctgggtatac tgggagtctt
      121 gagccagcta ggggtcgccg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca ggcgactggg gctgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012359
VERSION     KHUT01012359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacgtaggcc tcgagcgtta tccggattta ttgggcgtaa agagcatgta ggaggcttcg
       61 cgcgtctttg gttaaagacc accgcctaac ggtgggactg ccgaggatac ggcggagctg
      121 gaggaggtta gaggtgcata gaacgcatgg tgtaggggtg aaatccgttg atatcatgcg
      181 gaataccgaa ggcgaaggca gtgcactggg accttcctga ctctgagatg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01012360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012360
VERSION     KHUT01012360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctggg gtgaaagccc agggcttaac cctggaagtg ccccggaaac ggtgagactg
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctgga cagttactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012361
VERSION     KHUT01012361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctcgt
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg catacgaaac tggcgagctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcagac agg
//
LOCUS       KHUT01012362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012362
VERSION     KHUT01012362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           98 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctgga
       61 taagtagagg gtgaaatctc atggctcaac tgtgaggctg ccttctagac tgtctggctt
      121 gagcatggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gccagctggg ccattgctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012363
VERSION     KHUT01012363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggttctt
       61 taagtcctgt gtgaaagccc tgggctcaac ccaggaagtg cgcaggatac tggggaactg
      121 gagtggcgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgtcaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012364
VERSION     KHUT01012364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           82 g           57 t
ORIGIN      
        1 tacgaaggtg gcgaatgttg ctcggattca ctgggcgtaa agcgtctgta ggtggcttgt
       61 taagttagag gtgaaagccc cgcgctcaac gtgggaactg cctttaaaac tggcgagctt
      121 gagagtatca gaggtaggcg gaataactgg tgtagaggta aaattcatag atatcagttg
      181 gaacatccat ggcgaaggca gcttactggg atatttctga cactgagaga cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012365
VERSION     KHUT01012365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           96 g           41 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggccttg
       61 taagtccgtt ctgaaagccc ggggctcaac cccgggaggc ggatggatac tgcaaggctc
      121 gagttcggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012366
VERSION     KHUT01012366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta gtcggaatta ttgggcgtaa agggcgcgta ggcggttaat
       61 taagtgggat gtgaaagccc cgggctcaac ctgggaatgg cattccaaac tgattagcta
      121 gagtatagta gaggtgagtg gaatttctgg tgtagcagtg aaatgcgtag agatcagaag
      181 gaacaccagt ggcgaaggcg gctcactgga ctaatactga cgctgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012367
VERSION     KHUT01012367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatga ctgggcgtaa agggttcgta ggcgggagga
       61 caagttcagt gtgaaatccc caggcttaac ctgggacgtg cattgaaaac tgtctttctt
      121 gagtgttgga gagggtggtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaggcg accacctggc caaacactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012368             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012368
VERSION     KHUT01012368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           86 g           48 t
ORIGIN      
        1 tacagaggga gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggttcat
       61 taagtctact gtcaaatacc agggcttaac cttgggcagg cagtggagaa ctaatgagct
      121 agagtatggt agaggcagag ggaattccat gtggagcggt gaaatgcgta gagatatgga
      181 agaacaccag tggcgaaggc gctctgctgg gccaaaactg acactgagag acgaaagcca
      241 ggggagcaaa tagg
//
LOCUS       KHUT01012369             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012369
VERSION     KHUT01012369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctggg
       61 caagtcgggt gtgaaaactc caggctcaac ttggagacgc cacttgatac tgccctggct
      121 tgagtccggt aggggagcgc ggaattcctg gtgtagcggt gaaatgcgca gagatcagga
      181 ggaacaccgg tggcgaaggc ggcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012370
VERSION     KHUT01012370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcgtat
       61 catgtccggg gtgaaagccc ccagcttaac tggggaagtg ccttggaaac ggatgcgctc
      121 gagggaggta ggggtgtgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcgcactgga cctcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012371
VERSION     KHUT01012371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           83 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggatagt
       61 caagtcgtgt gtgaaatttc ggggctcaac ctcgaagcgg cacaggaaac taactatcta
      121 gagggtagat ggagagaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttctctaat ttattcctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01012372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012372
VERSION     KHUT01012372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           82 g           49 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtcaact gttaaaaccc ggggctcaac cccggaaccg cggttgatac ggcacgacta
      121 gaggatggca gagatgtatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtacattggg ccactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaaa agg
//
LOCUS       KHUT01012373             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012373
VERSION     KHUT01012373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           51 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtccaa
       61 caagtctggc gtgaaagcct tgagctcaac tcaagaagtg cgctggaaac tgtcggactt
      121 gagtactgga gggggaggtg gaattctcga gtgtagaggt gaaattcgta gatattcgga
      181 agaacatcag tggcgaaggc gacctcctgg acagatactg acgctgagac gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012374
VERSION     KHUT01012374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgac
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaacag cttctgatac tggtcgtctt
      121 gagtgaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012375
VERSION     KHUT01012375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 gacgaaccgt cctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggcttaac cgaggaactg cgctcaaaac taccgtgctt
      121 gagggagaca gaggtgagtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacaccggt ggcgaaagcg actcactggg tctctactga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012376             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012376
VERSION     KHUT01012376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agggcgcgta ggcggttacg
       61 caagtcggat gtgaaaactc caggctcaac ctggagacgc cacccgatac tgcgttgact
      121 tgagttcggt aggggatcac ggaatctctg gtgtagcggt gaaatgcgca gatatcagag
      181 ggaacaccgg tggcgaaggc ggtgatctgg gccgacactg acgctgatgc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012377
VERSION     KHUT01012377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           91 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agggagcgta ggcggtcttt
       61 taagttagat gtgaaaaacc cgggctcaac ttgggaggtg catctaaaac tgggggactt
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012378
VERSION     KHUT01012378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           95 g           48 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcatga
       61 caagtcagat gtgaaagccc cgggcttaac ccgggaactg catctgaaac tgttttgctt
      121 gagttctgga gggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagc ggcgaaggcg gctacctgga cagtgactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012379
VERSION     KHUT01012379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          104 g           41 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtggaacgg
       61 cgtgtccggt ctgaaagccc ccggctcaac cggggagggt ggctggaaac tgccgttctt
      121 gagggcgaga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccctctggc tcgaccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012380
VERSION     KHUT01012380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggattca ctgggcttaa agggtgcgta ggcggctgtt
       61 taagtagggt gtgaaagccc ccggctcaac cggggaattg cgctctaaac tggacagctt
      121 gagtgagaca ggggtgtgcg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tcttgactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012381
VERSION     KHUT01012381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           85 g           59 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ctgggcgtaa agggagcgta ggcgggctta
       61 taagttagga gtttaattca cgggcttaac tcgtgatctg cttttaatac tataggtctt
      121 gagtatggga gagggcgatg gaattctagg tgtagcggtg gaatgcgtag atatctagaa
      181 gaacaccagt agcgaaggcg gtcgcctggc ccaatactga cgctgaggct cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012382
VERSION     KHUT01012382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcggatatt
       61 taagtcagat gtgaaatccc ggggcctaac ttcggggctg catttgaaac tgggtatctt
      121 gagggcagga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgaccctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012383
VERSION     KHUT01012383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           83 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcgccg
       61 taagtcagtg gtgaaatact ctagctcaac tagaggggtg ccattgatac tgcggtgctt
      121 gagtgaagtc gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacaccaat agtgaagaca gcttactaga cttatactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012384
VERSION     KHUT01012384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 gacgaaccga gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtact
       61 caagtctgtg gtgaaatccg gcagcttaac tgtcgaactg ccgtggatac tgggtatctc
      121 gaggaaggta ggggcatgcg gaactattgg tggagcggtg aaatgcgttg atatcaatag
      181 gaactccggt ggcgaaggcg gcatgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012385
VERSION     KHUT01012385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           95 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggcggctcga
       61 gaagtctgat gtgaaagccc cgggcttaac ctgggaagtg cattggaaac tctcgggctt
      121 gagtgctgga gaggaaggga gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cccttctgga cagcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012386
VERSION     KHUT01012386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           73 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggttat
       61 taagtcagtg gtgaaatcct acagctcaac tgtagaactg ccattgatac tattaatctt
      121 gaatattgtt gaggtaggcg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gcttactaag caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012387
VERSION     KHUT01012387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           38 c           91 g           57 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttagtt gtgaaattcc tgggcttaac ctgggctggt caactaagac tgttagactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacactggt ggcgaaggcg actacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012388
VERSION     KHUT01012388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           90 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcataa agggcgcgta ggcgggccgt
       61 atagtcaggg gtgaaatcct ccagcttaac tggagaactg cctttgatac tgacggtctc
      121 gagtggggta ggggcgtgtg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg acacgctgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012389
VERSION     KHUT01012389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           79 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggcccga
       61 taagtcagtg gtgaaagccc gcagctcaac tgcggaattg ccattgaaac tgtcaggctt
      121 gaatatagtt gaagtgggcg gaatacaaca tgtagcggtg aaatgcttag atatgttgta
      181 gaacaccaat tgcgtaggca gctcactaag ctaatattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01012390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012390
VERSION     KHUT01012390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgatg
       61 taagtctgat gtgaaatctc agagcttaac tctgaaatgg cattggatac tatgttgctt
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga cgaatcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012391
VERSION     KHUT01012391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           94 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggtggttact
       61 taagtctgat gtgaaagctc ctggcttaac tgggagaggt cgttggaaac tgggtaactt
      121 gaggtgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcaccctggc attgacctga cgctcaggtg cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012392
VERSION     KHUT01012392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           85 g           47 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcta
       61 caagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcccaaaac tgtagtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacccgt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012393
VERSION     KHUT01012393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           80 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcgggttcg
       61 taagtcagtg gtgaaatcct acagcttaac tgtagaactg ccattgatac tgcgagtctt
      121 gagtgtagtt gaggtagctg gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaacaccaat tgcgaaggca ggttaccaag ttacaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012394
VERSION     KHUT01012394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           87 g           53 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatca ttgggcgtaa agcgtccgta ggcggttttt
       61 caagtctgct gttaaagcgt gcagcttaac tgcatatcgg cagtggaaac tgagagacta
      121 gagtgtagta ggggtagagg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg ctctactggg ctacaactga cgctgatgga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01012395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012395
VERSION     KHUT01012395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           82 g           55 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta ggcggtctat
       61 taagtttgtt gttaaatact tcggcctaac tgaagaaagg caacgaatac tggtaaacta
      121 gagggtgaaa gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggct gcttcttggt tcactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012396
VERSION     KHUT01012396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcacgca ggcggttcgt
       61 taagtcggtt gtgaaatccc acggcccaac tgtggggggt cgaccgaaac tggcggactt
      121 gagggaggca gagggaagcg gaattcccgg agtagtggtg aaatgcgtag ataccgggag
      181 gaacaccagt ggcgaaagcg gcttcctggg cttttcctga cgctgaggtg cgaaggcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012397
VERSION     KHUT01012397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggactcg
       61 aaagtacttt gtgaaagccc acggctcaac cgtggaaatg cttggtaaac tacgagtctt
      121 gaggtcggta ggggttgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcaactggg ccgaatctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012398
VERSION     KHUT01012398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           89 g           53 t
ORIGIN      
        1 tacgtaggtg acaagcgtta tccggaatta ctgggcgtaa agcgcacgca agcggtctga
       61 caagtctgac gtgaaatctc ttggcttaac caggagcggt cgttggaaac tgtcaggctt
      121 gaggcgatga gagggatgca gaattcctgg tgtagtggtg gaatgcgtag atatcaggag
      181 gaatacctgt ggcgaaagcg gcatcctggc gtcgtcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012399
VERSION     KHUT01012399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacgtactgg gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccttc
       61 caggtccggt gtgaaatctc tcggctcaac cgagaaatgg cgctggaaac cggaaggctt
      121 gagtaggcta ggggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg gcctaactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012400
VERSION     KHUT01012400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           68 c          115 g           26 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggccggc
       61 cgcgtcggac gtgaaagccc ccggctcaac cggggcgggt cgtccgcgac gggtcggctg
      121 gagggggcga gaggggggtg gaatgccggg tggagcggtg aaatgcgtag agacccggcg
      181 gaacaccagg ggcgaaggcg gccccctggc gccatcctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012401
VERSION     KHUT01012401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggttccg
       61 cgtgtcggat gtgaaatctg gaggctcacc ctccaaagtg cattcgaaac tgcgagactc
      121 gagtacagga gaggagagcg gaattcttgg tgtagcggtg aaatgcgttg agatcaagaa
      181 gaacaccagt ggcgaaggcg gctctctgga atgaaactga cgctgaggca cgaaagttgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012402
VERSION     KHUT01012402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           84 g           51 t
ORIGIN      
        1 tacagagacc acgagcgttg accggattta ttgggcgtaa agcgtgcgca ggcggtttcg
       61 taagtcattt gttaaatctt caggctcaac ctggaggcag cgggtgatac tgcgagacta
      121 gagaatggga gaggttggcg gaatgcccgg tgtaggggta aaatccgtta agatcgggta
      181 gaacaccaaa tgcgaaagca gccaactgga acatttctga cgctgaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01012403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012403
VERSION     KHUT01012403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa aggatgtgta ggcggtcttc
       61 taagtgggat gtgaaatccc tgggctcaac ccaggaattg cattccaaac tgggggacta
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012404
VERSION     KHUT01012404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           41 a           65 c          115 g           32 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggccgtg
       61 cgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac ggcatggctt
      121 gagggcggca ggggctggtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacacccgt ggcgaaggcg gccagctggg ccggctctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012405
VERSION     KHUT01012405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagac gtgaaatctc caggcttaac ctggaaactg cgtttgaaac tgggaggctt
      121 gaggaccgga gagggcagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cggcacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012406
VERSION     KHUT01012406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c          101 g           34 t
ORIGIN      
        1 aacgtaggag acaagcgtta tccggagtta ctgggcgtaa agggcgtgca ggcggcaggt
       61 taagatgggt gtgaaagcct ctggctcaac cgggggaggc cacccgtgac tgacccgcta
      121 gagggcggga gaggggtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcaccctagc ccgcacctga cgctgagacg cgacagcacg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012407
VERSION     KHUT01012407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           94 g           43 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggttctc
       61 taagtcggat gtgaaatcct acggcctaac cgtggaattg catccgatac tggggaactt
      121 gaggacggta gaggccggtg gaattcccgg tgtaccggtg aaatggacag atatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ccgatcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01012408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012408
VERSION     KHUT01012408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           88 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggcggtacgg
       61 aaagttggat gtgaaagctc ccggctcaac tgggagaggt cgttcaaaac ttccgcactc
      121 gagtacagta gagggaagtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcttcctgga ctgatactga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012409
VERSION     KHUT01012409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c          102 g           38 t
ORIGIN      
        1 gacggaggag gcaagcgttg tccggaatta ctgggcgtaa agcgctcgta ggcggtcgtc
       61 tacggcggtg gtgaaagctc ccggctcaac tgggagaggc ccgccgcaac gggacgactc
      121 gagggtggta gaggcagacg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gtctgctggg ccattcctga cgctgaggag cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012410
VERSION     KHUT01012410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           80 g           57 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agagtgtgta ggtggcacgt
       61 taagttgaat gttaaatctc agggctcaac tctggggctg cattcaatac tggcgagctc
      121 gaggtatgga gaggtaagcg gaattagtag tgtagcggtg aaatgcgtag atattactaa
      181 gaacaccaat ggcgaaggca gcttactggc cattacctga cactgagaca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012411
VERSION     KHUT01012411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           81 g           52 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca ggcggtatgc
       61 taagtcaact gttaaagacc tcggcctaac tgaggaacag cggtagatac tgacgtacta
      121 gagtatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccaat tgcgaaggca gcttcttggt ccattactga cgctaaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012412
VERSION     KHUT01012412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ctcggaatca ctgggcataa agggcgtcta ggcgggtcgg
       61 tcagtcggct gtgaaatccc tcggctcaac cgaggaagtg catccgatac tgccggtctt
      121 gagggcaaga ggggagagtg gaactcatgg tggagcggtg aaatgcgtag atatcatgag
      181 gaacgccggt ggcgaaagcg actctctggc ttgttcctga cgctgaagcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012413
VERSION     KHUT01012413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           87 g           51 t
ORIGIN      
        1 tacggagggt ccaagcgtta ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttac
       61 taagtctgat gtgaaatccc tagactcaat ctaggaagtg cattggatac tggtaggcta
      121 gagtacagga ggggaaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctttctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012414
VERSION     KHUT01012414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggaccat
       61 taagtcggag gtgaaagcct gaggctcaac ctcagaactg ccttcgatac tgatggtctt
      121 gagtttggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012415
VERSION     KHUT01012415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtctgg
       61 taagtgggat gtgaaagccc ccggctcacc cggggatctg cattccaaac tgccagactt
      121 gagtatggga gaggacaggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cctgtctggc ccaatactga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012416
VERSION     KHUT01012416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaacct
       61 taagtgtggc ttgaaagccc ccggctcaac cggggaaggt ggctgcaaac tggggttctt
      121 gagggcgtga gaggagcgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcgctctggc gcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012417
VERSION     KHUT01012417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           95 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgca ggcggttata
       61 caagtgaggt gtgaaatccc cgggcttaac ccgggaactg catttcagac tgtatggctg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012418
VERSION     KHUT01012418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggtgtaa agcgtgtgca ggcggtcatg
       61 taagtagcgc gtgaaagcgc ccggcttaac cgggcgagga cgtgctgtac tgcatgactg
      121 gaggctggta gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg actcgctggg ccagcactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012419
VERSION     KHUT01012419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           92 g           46 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtcctt
       61 taagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tgggggactt
      121 gaatactgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccatctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012420
VERSION     KHUT01012420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattca ttgggcgtaa agagcttgta ggtggccctg
       61 taagttggac gtgaaaggcc cgggcttaac ccgggacggc cgttcaatac tgcggggctg
      121 gagggcgtca gaggggagtg gaatccctgg tgtagcagtg aaatgcgtag atatcagggg
      181 gaacacccgt ggcgaaggcg gctccctggg acgcccctga cactgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012421
VERSION     KHUT01012421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           92 g           44 t
ORIGIN      
        1 aacgtaggag ccaagcgtta tccgaattta ctgggcgtaa agcgcgtgta ggcggcacgg
       61 taagttggat gtgaaatctc ccggctcaac tgggagaggc cgttcaagac tgccgagctg
      121 gaggatggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ccaaccctga cgctcagacg cgaaagctag
      241 gggagtgaac ggg
//
LOCUS       KHUT01012422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012422
VERSION     KHUT01012422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 aaagtctgat gtgaaatccc cgggcccaac ccgggaaatg catcggaaac tgcctggctt
      121 gagggttgga ggggggactg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacgccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012423
VERSION     KHUT01012423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           80 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtccgta ggcggattag
       61 taagtcagtg gtgaaagccc gcagctcaac tgtggaactg ccattgatac tgctagtctt
      121 gagtgtagtt gaagtagctg gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaacaccaat tgcgaaggca ggttactaag ttacaactga cgctgatgga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012424
VERSION     KHUT01012424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgta ggcggtttcc
       61 taagtcagat gtgaaaaccc cgggcttaac ctgggacctg catttgaaac tgggagacta
      121 gagtacagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012425
VERSION     KHUT01012425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           81 g           51 t
ORIGIN      
        1 tacgagtacc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggtctgg
       61 ttagtcttct gtcaaaattt cgggctcaac ccgaaacctg cggaggaaac ggccaaactt
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca attcactggc gcatttctga cgctgaaaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012426
VERSION     KHUT01012426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           86 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatgtt
       61 taagtcagat gtgaaaatcc cgagcttaac ttgggaactg catttgaaac tggatatcta
      121 gagtgcggga gaggagaatg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg attctctgga ccgtaactga cgctgaggca tgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012427
VERSION     KHUT01012427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaataa ctgggcgtaa agggcacgca ggcggtgact
       61 taagtgaggt gtgaaagccc cgggcttaac ctgggaattg catttcatac tgggtcgcta
      121 gagtacttta gggaggggta gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccgaa ggcgaaggca gccccttggg aatgtactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012428
VERSION     KHUT01012428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcataa agcgcgcgta ggcggctccg
       61 taagtgcggt gtgaaatccc acggctcacc cgtggaactg cgccgtatac tgcagggcta
      121 gagcatggta gaggagggcg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctggt ccattgctga cgctgaggcg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012429             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012429
VERSION     KHUT01012429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           53 c           80 g           50 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ctgggcgtaa agggtccgca ggtggtcctg
       61 cacgtcgatg gttaaatgta cgggctcaac ccgtacgccg ccgtcgatac ggcagtgact
      121 agagcatgga agaggtaagc ggaattaccg gtgtagtagt aatatgcgtt aatatcggta
      181 agaacaccaa aagcgtaggc agcttactag gacattgctg acactcaggg acgaaagcgt
      241 ggggagcgaa aggg
//
LOCUS       KHUT01012430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012430
VERSION     KHUT01012430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           95 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttgag
       61 caagtcggat gtgaaagccc tgggcttaac ctgggaattg cattcgagac tgctcggcta
      121 gagtttggta gaggaaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gctttctgga ctgaaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012431
VERSION     KHUT01012431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcgatttga
       61 taagtctcgt gtgaaagccc agggcctaac cctggaaggg cacgggatac tgttgaattt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagc ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012432             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012432
VERSION     KHUT01012432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           78 g           60 t
ORIGIN      
        1 tacagaggtt acaagcgtta tccggattta ttgggcgtaa agcgtttcgt aggcggtctg
       61 aaaagttact tttcaaatac cacggctcaa ccgggggaag ggaggtaata ctggcagact
      121 tgattattgg tggggtttct agaactgatg gtgtagtagt gaaatacgtt gatatcatca
      181 ggaattccaa gggcgaaggc aggaaactaa ccaattaatg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01012433             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012433
VERSION     KHUT01012433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           83 g           53 t
ORIGIN      
        1 tacggaggaa gcaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcaaa
       61 gtgtgtcgat agtgaaagcg tggggctcaa cctcatatcc attatcgaaa ctgcttagct
      121 agaggacgag agaggttatt ggaattccca gtgtaggagt gaaatccgta gatattggga
      181 ggaacaccga tggcgtaggc agataactgg ctcgttcctg acactaaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012434
VERSION     KHUT01012434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           88 g           50 t
ORIGIN      
        1 tacggaggtc ccaagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggactgg
       61 caagtctaaa gtggaatccc ggggctcaac ctcggaactg cttcggatac tatcagtctt
      121 gagttcgtga gaggaaagcg gaattcaggg tgtagcggtg aaatgcttag atatcctgag
      181 gaacaccaat ggcgaaggca gctttctggc gcgatactga cgctgaggct cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012435
VERSION     KHUT01012435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           94 g           48 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatca ttgggcgtaa agggtatgta ggcggtgcgg
       61 taagtctggt gtgaaatccc caagcttaac ttgggatccg cgccagagac tgccgtgcta
      121 gagtgtggga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctttctgga ccactactga cgctgagata cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012436
VERSION     KHUT01012436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaataa ctgggcgtaa agggcgcgta gacggtatat
       61 taagtctgtt gttaaatttt ctggcccaac cagaagtcag cagcggatac tggtatgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012437
VERSION     KHUT01012437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           96 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggagtgta ggcggttcgg
       61 taagtgggat gtgaaatgcc cgggctcacc ccgggacctg catctcatac tgctgagctt
      121 gagtacagga gagggtgggg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg cccacctggc ctgctactga cgctgatgct cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012438
VERSION     KHUT01012438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           37 c           89 g           58 t
ORIGIN      
        1 tacggggggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttttt
       61 taagttatct gtgaaattac tgggcttaac ctggttagga aagataatac tgagagacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012439
VERSION     KHUT01012439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcatg
       61 caagtcaggg gtgaaagccc ccggcccaac cggggaagtg cctctgatac tggatgcctc
      121 gagcgggata ggggcagctg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga tccctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012440
VERSION     KHUT01012440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctcag
       61 caagtcagac gtgaaatccc tcagcttaac tgaggaactg cgtctgatac tgctgggctt
      121 gagtgtcgga ggggatggta gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaatacccgt ggcgaaggcg accatctgga agacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012441
VERSION     KHUT01012441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           95 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtcttg
       61 taagtctgtt gtgaaatctc ccggcttaac cgggagggtg cggtggaaac tgcaaggctg
      121 gagtatggga gaggtaggcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtagacg gcttactgga ccatcactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012442
VERSION     KHUT01012442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggttgataac
       61 caagtgagtt gtgaaatccc tgggcttaac ctaggaattg caacttagac tggttgtctg
      121 gagtgcagta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgcctgga ctgagactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012443
VERSION     KHUT01012443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           77 g           64 t
ORIGIN      
        1 tacggaggat cctagcgttg tccggattta ttgggtttaa agggtgcgta ggcggattga
       61 taagtcaggg gtgaaagttt gcagcttaac tgtaaaattg cctttgaaac tgccaatctt
      121 gagtacagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gctcactaaa ctgtaactga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012444
VERSION     KHUT01012444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           84 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgtgta ggcgggactt
       61 taagtagcaa gtgaaaggtt ggtgctcaac accaacactg ctcgctatac tggagacctt
      121 gagtgccgga gaggaagatg gaactgcacg tgtagcggtg aaatgcgttg agatgtgcag
      181 gaacaccaat ggcgaaggca atcttctaga cggaaactga cgctgagaca cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01012445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012445
VERSION     KHUT01012445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtcag
       61 taagcccgtg gtgaaatctc caagcttaac ttggaaactg ccgtggatac tattgacctt
      121 gaatgttgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca caaatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012446
VERSION     KHUT01012446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           85 g           51 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgtgta ggcggtttag
       61 caagttagaa gtgaaagccc ggagcttaac tccggaactg cttttaaaac tactaatcta
      121 gagtacggta gggggtgatg gaattcctag tggagaggtg aaattcttag atattaggag
      181 gaacaccgaa ggcgaaagca atcacctggg ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012447
VERSION     KHUT01012447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaagta ggtggtctca
       61 tttgtctggg gtgaaatcct tgggcttaac tcaagaagtg ccccagaaac ggtgagacta
      121 gagtcctaga gagggtcgtg gaatacccgg tgtagcggtg aaatgcgtag agatcgggtg
      181 gaacaccaga ggcgaaggcg gcgacctgga taggtactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012448
VERSION     KHUT01012448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           61 c          103 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggactgc
       61 cgcgtctggg gtgaaatcct ccggctcaac cggagaagtg cctcgggtac gggcggtctc
      121 gaggggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012449             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012449
VERSION     KHUT01012449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggttaga
       61 taagttgtct gtgaaagccc tgggcttaac ccaggaaggt caggcaaaac tgtttagctt
      121 agagtacaag agagggtagt ggaatttccg gtgtagcggt gaaatgcgta gatatcggaa
      181 ggaacaccag tggcgaaggc ggctacctgg cttgatactg acactgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012450             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012450
VERSION     KHUT01012450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c          101 g           39 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggcccgt
       61 taggttccat cgtgaaaggc aggggctcaa cccctggact gcgaggaaaa ccggcaggct
      121 ggagagaggc agaggaaggt ggaatggctg gtggagcggt gaaatgcgta gagatcagtc
      181 ggaacaccca aggcgaaggc agccttctgg gcctcatctg acgctgtgac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012451             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012451
VERSION     KHUT01012451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           47 c          101 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggctttt
       61 taagtctggg gtgaaaggtt ctgcgcttaa cgtgaacagt gccctggaga ctggaaggct
      121 tgagtgtggg agggggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctgcctgg cctaacactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012452             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012452
VERSION     KHUT01012452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           82 g           57 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa agtgtgcgta ggtggtttaa
       61 taagttactg gtggcaaatc ggggcccaac cccgtggaag ttagtaatac tattagacta
      121 gaggactgga gaggttaacg gaatgattcg tgtaggggta aaatccgtaa atatgaattg
      181 gaacaccaaa cggcgaaggc agttaactat tcggtgcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01012453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012453
VERSION     KHUT01012453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctata
       61 taagtcaggt gtgaaagccc ggggctcaac ctcggaagtg catttgatac tgcatagctt
      121 gagtgcggga gaggctatta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccgga ggcgaaggcg gatggctggc ccgacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012454             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012454
VERSION     KHUT01012454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           78 g           60 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta ggtggtagaa
       61 taagttactg gttgaagtca agctcaactt gacggaagct ggtaaaactg ttctgctaga
      121 ggttaggaga ggttgacgga actctataag taagggtgaa atctgttgat ctatagagga
      181 ccaccaaaaa aggcgaaggc agttaactat tctattcctg acactgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01012455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012455
VERSION     KHUT01012455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           81 g           52 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtttttgat
       61 cacgtctggc agtaaattgc ggggctcaac cccgcatccc tgctggaaac gagttggata
      121 gagtcattca gaggcatctg gaatgtcatg tgtaggggta aaatccgtag atacatgatg
      181 gaacgccaaa agcgaaggca ggatgctagg aatgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01012456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012456
VERSION     KHUT01012456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           94 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggattta ctgggtgtaa agggcgtgta ggcgggatgg
       61 caagtcaggc gtgaaaacta tgggcttaac ctgtagcttg cgtttgaaac tgtgattctt
      121 gagtgatgga gaggcaggcg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagt ggcgaaggcg gcctgctgga cattaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012457
VERSION     KHUT01012457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 ccagtctggg gtgaaatccc gcggctcaac cgtggaaccg cctcagatac tgacggcctc
      121 gagggagata ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaggcg gcgtgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012458
VERSION     KHUT01012458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          100 g           45 t
ORIGIN      
        1 gacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggctaag
       61 gcagttggat gtgaaagccc ggggctcaac cccggaggtg catccgatac tccttggctg
      121 gaggccggaa gaggagggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaagcg gctctctggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012459
VERSION     KHUT01012459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           51 c           83 g           45 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccagaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 caagtctgcc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttccgg tggagcggtg aaatggattg agatcggaaa
      181 gaacaccaac ggcgaaatca ctctgctggg ccaacactaa cactgagaga tgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01012460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012460
VERSION     KHUT01012460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 taagttctgt gtgaaagccc tgggctcaac ccaggaagtg cgcagaatac tgcctgactg
      121 gagtgacgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgtcaactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012461
VERSION     KHUT01012461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 tacgtatggt gcaaacgttg ttcggaatca ctgggcttaa agagcacgta ggcggtcagg
       61 aaagtctgat gtgaaatccc ttggcttaac tgaggaactg catcggatac ttcctgacta
      121 gagtgcagga ggggagagtg gaattcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggc ctgttactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012462
VERSION     KHUT01012462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggtggctttg
       61 tgtgtcaggt gtgaaatccc ggggcttaac cccggaactg cgcctgaaac tgcagagcta
      121 gagcattgga gagggaagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg acttcctgga caattgctga cactcaggca cgaaagcatg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01012463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012463
VERSION     KHUT01012463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           90 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcatgta ggcggttaga
       61 taagttggat gtgaaaggct acggctcaac cgtagagctg catccaaaac tgtttaactt
      121 gagtgcagta gaggaaagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actttctggg ctgtaactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012464
VERSION     KHUT01012464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           93 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcatgta ggcggttatg
       61 ttagtctgat gtgaaatacc cgggcttaac ctgggatatg cgttggagac ggcatagcta
      121 gagtatggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacactcgt ggcgaaggcg gctttctggc ccataactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012465
VERSION     KHUT01012465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           93 g           44 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgg
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgccgacctt
      121 gaggcactta ggggcgagcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgac ggtgaaaaca gctcgctggg agtgtcctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012466
VERSION     KHUT01012466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           82 g           52 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggtccta
       61 ttagtcttct gtcaaatttc ccggcttaac cgggaacctg cagaggaaac ggtaggactc
      121 gaggatgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca attcactggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012467
VERSION     KHUT01012467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          108 g           37 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggttact
       61 cgcgtcgcac gtgaaagccc ccggctcaac cggggagggt cgtgcgagac ggagtaactg
      121 gaggcagaga gaggctggtg gaattcccgg agtagtggtg aaatgcgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gccagctggc tctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012468
VERSION     KHUT01012468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          106 g           36 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatca ctgggcgtaa agcgcgcgta ggtggcacag
       61 tgcgtcggtg ctgaaagcgc cgggcttaac ccggcgaggt gcagcgatac gactgagctc
      121 gaggccagca gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgccggt ggtgaagacg gccacctggg ctggacctga cactgaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012469
VERSION     KHUT01012469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          108 g           37 t
ORIGIN      
        1 tacgtaggga ccgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggtcggg
       61 tgcgtcgggt gtgaaagccc tccgctcaac ggaggagagt cgctcgaaac ggtccgactg
      121 gaggcagggc gaggtcggtg gaattcccgg tgtagtggtg atatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gccgactgga cctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012470
VERSION     KHUT01012470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcgggtttt
       61 taagtcaggg gtgaaatccc aaggctcaac cttggaactg cctttgatac tggaaatctt
      121 gagtccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012471
VERSION     KHUT01012471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           60 c           92 g           42 t
ORIGIN      
        1 tacgtaggtt gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtcctg
       61 taagtccatg gtgaaagctc ccggcccaac tgggagaggc ccgtggaaac tacaggactt
      121 gaaggcggga gaggcagatg gaatttccgg tgtagcggta aaatgcgtag ataccggaag
      181 gaacaccagt ggcgaaagcg atctgctggc ccgcccttga cgctcaggcg cgaaagctag
      241 gggagcgacc ggg
//
LOCUS       KHUT01012472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012472
VERSION     KHUT01012472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 aacgtaggag acgaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtctga
       61 taagttcggc gtgaaatttc tcggcttaac cgggggatgt cgtcggagac tgtcggacta
      121 gagggcgata gaggtgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacactggg tcgcacctga cgctaagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012473
VERSION     KHUT01012473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           91 g           43 t
ORIGIN      
        1 gacagggggc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggtcgg
       61 taagcatccc gtgaaatccc ttggctcaac caaggaaccg cgggatggac tgccgatctt
      121 gaggaagcta gaggcgggta gaacgatagg tggagcggtg gaatgcgtag atatctatcg
      181 gaatgccgaa agtgaagaca gcccgctggg gctttcctga cgctaaagca cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012474
VERSION     KHUT01012474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c           99 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtca ttcggaatca ctgggcttaa agggcgcgta ggcgggtgag
       61 cgagtccggg gtgaaatccc acggcccaac cgtggaaggg cctcggatac cgctcgtctc
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg acacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012475
VERSION     KHUT01012475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcacgcgta ggcggcttgg
       61 taagttggga gtgaaatccc gaggcttaac ctcggaattg ctttcaaaac tatcaagcta
      121 gagtatggca ggggacagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaagcg actgtctgga ccattactga cgctgaggcg tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012476
VERSION     KHUT01012476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcggag
       61 taagtcagac gtgaaatccc tcggctcaac cgaggaactg cgtctgaaac tgctctgctt
      121 gaggccggga gaggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccggttctga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012477
VERSION     KHUT01012477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           91 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgtacgca ggcggctcct
       61 taagtcgggt gtgaaagccc agggctcaac cctggaactg cgcccgaaac tgggaagctt
      121 gagtgcggga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttcctggc ccgacactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012478
VERSION     KHUT01012478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtctca
       61 aaagtcgggt gtgaaagccc tgggctcaac ccaggaagtg cactcgaaac tgcgaggcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012479
VERSION     KHUT01012479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           92 g           46 t
ORIGIN      
        1 tacagggggc tcgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggtccat
       61 caagtgttct gtgaaatccc cgagctcaac tcgggaactg cagggcaaac tgaaggactt
      121 gaagcaggta ggggctggaa gaacagttgg tggagcggtg aaatgcgtag atatcaactg
      181 gaatgccgat ggggaagccg tccagctggg cctgtcttga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012480
VERSION     KHUT01012480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           62 c           97 g           38 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggtcgcg
       61 cgcgtggggt gtgaaaccgg gcggcttaac tgcccgacgc catcccatac ggcgcgactc
      121 gagcattgga gaggtccatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggc cagttgctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012481
VERSION     KHUT01012481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           94 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgtgta ggtgggaaga
       61 taagtcagac gtgaaaggcc tgggcttaac ctaggaatgg cgtatgaaac tgtttttctg
      121 gagtcgagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctctgactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012482
VERSION     KHUT01012482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggagcgca ggcgggaagc
       61 caagttggat gtgaaatacc gcagcttaac tgcggggctg catccaaaac tggttttctt
      121 gagtgaagta gaggtaggcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcctactggg ctttaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012483
VERSION     KHUT01012483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggctgatacg
       61 tgtgtcaggt gtgaaatccc tgggctcaac ccaggaactg cacttgaaac tgcgtatcta
      121 gagtgtcgga gagggaagcg gaattgctgg tgtaggggtg aaatccgtag atatcagcag
      181 gaacatcggt ggcgaaggcg gcttcctgga cgactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012484
VERSION     KHUT01012484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           40 c           97 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agcgcctgta ggtggtttgt
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tggcggactg
      121 gagtcggaca gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaatatcgat ggcgaaggca gcgacctggg tccagactga cactgagggg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012485
VERSION     KHUT01012485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           93 g           42 t
ORIGIN      
        1 tacggaaggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggataag
       61 aaggcatctc gtgaaagccc ccggctcaac cggggaactg cggggtgaac cacttgtctt
      121 gaggaaggta ggggtcagtg gaaccatggg tggagcggtg aaatgcgtag atatccatgg
      181 gaacgcctat ggcgaaggca actgactggg cctttcctga cgctcaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012486
VERSION     KHUT01012486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           87 g           57 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggttagg
       61 taagtcaggt gtgaaatccc tgggctcaac cgaggaagtg cacttgaaac tacctagcta
      121 gaggacagta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaatacctgt ggcgaaggcg gccttctggg ctgttcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012487
VERSION     KHUT01012487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           94 g           50 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcag
       61 caagtctggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tgctggtcta
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012488
VERSION     KHUT01012488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggttgca
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaattg cgttgaagac tgtggaacta
      121 gagtcggaca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgacctggg tccagactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012489
VERSION     KHUT01012489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcggtg gtgaaatcct tcagcttaac tggagaatgg ccgccgatac tgacgacctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012490
VERSION     KHUT01012490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           82 g           55 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggaattc
       61 caagtcagcg gtgaaatctc catgctcaac atggacactg ccgttgaaac tggcgttcta
      121 gagtgtaaat gaggtaggcg gaatgcgtgg tgtagcggtg aaatgcatag atatcacgca
      181 gaactccgat tgcgaaggca gcctgctggg atacaactga cgctgaggca cgaaagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01012491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012491
VERSION     KHUT01012491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           54 c          110 g           41 t
ORIGIN      
        1 tacgggggat gcgagcgttg tccggattca ctgggcgtaa agggcgcgta ggcggcctgg
       61 tcagtccgat ttataagtgc ggggctcaac cccgtgatgg attggggacg gtcaggctgg
      121 ggcgcggcag agggagacgg aatgccgggt gtagtggtga aatgcgtaga gatccggcgg
      181 aacaccggtg atgaagatgg tctcctgggc cgagggccga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012492
VERSION     KHUT01012492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           98 g           39 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgggca ggcggtctag
       61 caagtggggt gtgaaatctc ccagcttaac tgggagaggc cattccaaac tgctggactg
      121 gagggcagta gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgcacctga cgctgaaccg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012493             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012493
VERSION     KHUT01012493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           64 c          102 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggc
       61 cgcgtcgttt gctgaaagcc cccggctcaa ccgggggacc ggcggcgaaa cgggccgtct
      121 ggaggggcgt agggggagct ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg cggcgaaagc gagctcctgg acgcctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012494             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012494
VERSION     KHUT01012494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           62 c           85 g           43 t
ORIGIN      
        1 caccagcgcc acgagtggcc atcacgatta ttgggcctaa agcgttcgta gtcggttcag
       61 tgcatcctct gtgaaatcgc agcgcctaac gttgcggcat gcggaggaca cgactgagct
      121 tgagaccggg aggcgtcgag ggtattccaa ggggaccggt aaaatggtat aatccttgga
      181 ggaccacctg tggcgaaggc gctcgacgag aacggatctg acgatgagga acgaaagcca
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01012495             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012495
VERSION     KHUT01012495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           93 g           49 t
ORIGIN      
        1 tacggagagg gcaagcgttg ttcggattta ttgggcgtaa agcgcgtgta ggcggttcag
       61 taagtcagat gttaaagacc cacggctcac ccgggggcct gcgtctgata ctgctggact
      121 agagtacgaa agaggagagc ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacccg tggcgaaggc ggctctctgg ttcgtaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012496             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012496
VERSION     KHUT01012496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           49 c           79 g           52 t
ORIGIN      
        1 tacggagaac tcaagcgtta cccggaatca ctgggcgtaa agcgttgcgt aggcggtttg
       61 ttaagtgaga tatgaaatgt tacggctcaa ccgtgaaagt gtatctcaaa ctaacaaact
      121 agaggatggt agaggtcagt ggaattgctg gtgtaggagt gaaatccgta gatatcagca
      181 ggaacaccaa tggcgtaggc agctgactgg accactcctg acgctcaggc acgaaagcgt
      241 gggtagcgaa cggg
//
LOCUS       KHUT01012497             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012497
VERSION     KHUT01012497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           43 t
ORIGIN      
        1 tacagagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggaacgt
       61 gcaagtccga agtgaaatct cgccgctcaa cggcgaggct gctttggaga ctgtcgatct
      121 agggaccggt agaggctggt agaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaatacccg tggcgaaggc ggccagctgg gccgggtccg acgctgatgt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012498             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012498
VERSION     KHUT01012498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           99 g           41 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggtggtcgag
       61 gcaagtgcaa tgtgaaagcg gcccgcttaa cgggtcgagg ccatggcaga ctgcccgact
      121 ggaggtcgtg agaggtccgt ggaattgccg gtgtagtggt gatatgcgta gatatcggca
      181 ggaacaccca aggggaagcc agcggactgg tacgatgctg acactgaggt gcgacagcgt
      241 ggggagcgaa ctgg
//
LOCUS       KHUT01012499             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012499
VERSION     KHUT01012499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          100 g           45 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggacttc
       61 caagcctgag gtgaaaggtc ggggctcaac cccgtacagt gccttgggga ctgtgagtct
      121 ggaggcaggc agaggagtgt ggaattcctg gtgtagcggt ggaatgcgta gagatcagga
      181 agaacaccca tggcgaaggc agcactctgg gcctgtactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012500             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012500
VERSION     KHUT01012500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           46 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 taagtcaggt gtgaaaactc cgagctcaac tcggagacgc catctgatac tgccatggct
      121 agagtccggt aggggagcgc ggaattcctg gtgtagcggt gaaatgcaca gatatcagga
      181 ggaacaccga tggcgaaggc agcgctctgg gccggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012501
VERSION     KHUT01012501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           91 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggagcgta ggcggacatt
       61 taagtgggat gtgaaatact cgggctcaac ccgggggctg cattccaaac tggaagtcta
      121 gagtgcagga gaggagagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actctctgga ctgtaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012502             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012502
VERSION     KHUT01012502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           86 g           56 t
ORIGIN      
        1 tacgtggggt gcaagcgtta tccggattta ctgggcgtaa agcgtatcgt aggtggatat
       61 atgcgtggcg ggtttaagac cttggcccaa ccaaggaagt gctcgtcata cggtatatct
      121 tgagtctgtt cgagggaact ggaattctgt gtggaggggt gaaatccgta gatatacaga
      181 ggaacgccaa gggcgaaggc aggttcctag ggcagtactg acactcatga acgacagcta
      241 ggggagcaaa aggg
//
LOCUS       KHUT01012503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012503
VERSION     KHUT01012503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttg
       61 taagtcaggg gtgaaagcct ggagctcaac tccagaactg cctttgatac tgcatggctt
      121 gaatccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgggattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012504
VERSION     KHUT01012504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           79 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcggatttt
       61 taagtcagcg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tattagtctt
      121 gaatcttgtt gaggtaggcg gaacgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacaccgat tgcgaaggca gcctactaag caagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012505
VERSION     KHUT01012505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          104 g           42 t
ORIGIN      
        1 gacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgta ggcggcacag
       61 caagcctggc gtgaaagggt gcggcttacc tgcacgaggg cgttggggac tgctgagcta
      121 gaatccggtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgcg ggcgtaagca gccgactagg gcgagattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012506             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012506
VERSION     KHUT01012506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggcggcg
       61 taagttcgtg gtgaaatccc atcggctcaa ccgtggaatc gcttcgaata ctgcgtcgct
      121 cgagtgggat aggggcttgt ggaactgccg gtggagcggt gaaatgtgtt gatatcggcg
      181 ggaacgccag tggcgaaagc ggcaagcttg accccaactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012507             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012507
VERSION     KHUT01012507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           51 c           81 g           44 t
ORIGIN      
        1 gacagaggat gcaaacgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 caagtccgcc gtcaaatccc agggctcaac cctggacaga cggtggaaac taccaagcta
      121 gaatatggta ggggcagagg gaatttccgg tggagcgatg aaatgcattg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgttggg ccgaaactga cactgagaga cgaaaagcta
      241 ggggagcaaa tggg
//
LOCUS       KHUT01012508             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012508
VERSION     KHUT01012508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           85 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctacc
       61 ataagtttgg tgtgaaagct ccgggctcaa cccggaaatg gcattgaata ctaaggagct
      121 agaggattgg aggggagact ggaatacttg gtgtagcagt gaaatgcgta gatatcaagt
      181 ggaacaccag tggcgaaggc gagtctctgg acaatacctg acactgaggc acgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012509
VERSION     KHUT01012509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggcatc
       61 taagtcaggg gtgaaatgcc agggctcaac cttggaattg cctttgatac tggatgcttg
      121 gagttcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012510
VERSION     KHUT01012510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctacc
       61 taagtcagat gtgaaagccc tcggctcaac tggggaagtg catctgaaac tgagtggctt
      121 gagtatcgaa gaggatcgcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggc ggcgaaggcg gcgatctggt cgaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012511
VERSION     KHUT01012511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcacac
       61 taagtcaggc gtgaaagccc ccggcttaac cggggaacgg ctcctgatac tggtgtgcta
      121 gaggacggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtttctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012512
VERSION     KHUT01012512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagac gtgaaaggtc agggcttaac cctgggactg cgtttgagac tgaagggctt
      121 gaggatcgga gagggcagtg gtattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg actgcctgga cgacacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012513
VERSION     KHUT01012513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           63 c          110 g           35 t
ORIGIN      
        1 cacgtggggt ccgagcgttg tccggagtca ctgggcgtaa agcgcacgca ggcggttcac
       61 tgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggtgaactt
      121 gagagcggca ggggccggtg gaatccctgg tgtagtggtg aaatgcgtag agatcagggg
      181 gaacacccgt ggcgaaggcg gccggctggg ccgactctga cgctgaggtg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012514             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012514
VERSION     KHUT01012514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ctcggaatca ctgggcttaa agcgcgcgta ggcggcgaca
       61 taagtcaggg gtgaaatccc atcggctcaa ccgtggaact gcctttgata ctgtgttgct
      121 tgagggaggt aggggctcgc ggaacttccg gtggagcggt gaaatgtgtt gagatcggag
      181 ggaacgccag tggcgaaagc ggcgagctgg atctcttctg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01012515             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012515
VERSION     KHUT01012515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           66 c           99 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcctgg
       61 agcgtcagcg gctgaaatcc cccggctcaa ccggggaacg ggccctgaaa cgtccaggct
      121 ggaggggggt agggggagcc ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gggctcctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012516             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012516
VERSION     KHUT01012516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggagtta ctgggcgtaa agagcgcgta ggcggcttat
       61 taagttgact gttaaattcc ctggctcaac cggggctaag cggtcaatac tgatggagct
      121 agaggttagg agagagaagt ggaattcttg gagtagcggt aaaatgcgta gatctcaaga
      181 ggaacaccga ttgcgaaggc agcttcttgg cctatacctg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012517             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012517
VERSION     KHUT01012517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           82 g           58 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agagtgcgta ggtggctgaa
       61 taagttattg gtgggaaatc ggggcccaac ctcgtggaag ctagtaatac tattcagctt
      121 gagagccgga ggggttaacg gaattctatg tgtagcggta aaatgcgtaa atatatagag
      181 gaacaccaat tggcgaaggc agttaactat tcggcttctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01012518             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012518
VERSION     KHUT01012518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           84 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agggggcgta ggcgggaagg
       61 aaagtcaatc cgtgaaatcc cagggctcaa ccctggaacg gcggttgaaa cttcctttct
      121 tgagggtaga cggagaaaac agaattccac aagtagcggt gaaatgcgta gatatgtgga
      181 agaataccgg tggcgaaagc ggttttctag tttatacctg acgctgaggc ccgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012519             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012519
VERSION     KHUT01012519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaattta ttgggcgtaa agcgcgtgta ggcggaccat
       61 caagcctgag gtgaaaggtt ggggctcaac cccatacagt gccttgggga ctgttggtct
      121 ggaggcaggc agaggtatgt ggaattcctg gtgtagcggt ggaatgcgta gagatcagga
      181 agaacaccca tggcgaaggc agcatactgg gcctgtactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012520             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012520
VERSION     KHUT01012520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatagg
       61 cgcgtcgtat gttgaaagcc tccggctcaa ccggagaatc ggcggcgata cggcctgtct
      121 agaggaacgc aggggaatct ggaacttccg gtggagcggt gatatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagattctgg acgttttctg acgctgagac gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012521             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012521
VERSION     KHUT01012521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           50 t
ORIGIN      
        1 tacggaggga gcaagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggcttgt
       61 taagtgtggt gtgaaatctc cagggctcaa cccggaaacc gcaccgcata ctggcaagct
      121 agaggaatgt agaggtagat agaattcccg gtgtaacggt gacatgtgta gatatcggga
      181 ggaataccaa tggcgaaggc agtctactgg acatttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012522             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012522
VERSION     KHUT01012522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           94 g           53 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgta ggcggtttgg
       61 caagtcggat gtgaaaactc caggcttaac ctggagatgc cattcgatac tgctatgact
      121 tgagttcggt aggggcgcat ggaatacccg gtgtagcggt gaaatgctta gatatcgggt
      181 ggaacaccag tggcgaaggc ggtgcgctgg gccgaaactg acgctgatga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012523             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012523
VERSION     KHUT01012523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           64 c          101 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgc
       61 cgcgtcggtc gctgaaatcc cccggctcaa ccggggaagt ggcaccgaaa cgggcggcct
      121 ggagggaggt agggggggcc ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gggcccctgg accttttctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012524             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012524
VERSION     KHUT01012524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           43 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcagct gtgaaagctc cgggctcaac ccggagaggc cagctgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaactcctg gtgtagcggt gaaatgcgca gatatcagga
      181 agaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01012525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012525
VERSION     KHUT01012525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcttgg
       61 caagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tgccgagctt
      121 gagtatcgga gaggtgggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gcccactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012526
VERSION     KHUT01012526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          104 g           39 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgcgta ggcggctcgg
       61 taagtgggtc gtgaaagcgc ccggctaaac tgggcgagga cgatcgagac tgccaggcta
      121 gagggtggta gagggacatg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggcgaaggcg atgtcctggg ccacctctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012527
VERSION     KHUT01012527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           81 g           58 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattga ttgggtgtaa agggtgtgca gacggcttac
       61 taagtcactt gttaaattct tcggcctaac tgaagatctg caggtgatac tggaaagcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgttcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012528
VERSION     KHUT01012528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           91 g           43 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcgggaata
       61 ttagtcaagc gtgaaagccc caggcttaac ctgggaacag cgcatgaaac ggtataccta
      121 gaggacgaga gaggtaagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccaga ggcgaaggcg gcttactggc tcgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012529
VERSION     KHUT01012529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagcgcgca ggcggccatt
       61 caagtcagat gtgaaagacc ccggcttaac cggggctagt cgtctgaaac tggatagcta
      121 gagtggcaga gaggaaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctttctggc tgctaactga cgctgaggcg cgaaggctgg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012530
VERSION     KHUT01012530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           84 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggattta ctgggtgtaa agggcgagta gacggcgtta
       61 caagtcagat gtgaaaactt ggggctcaac ctcaagactg catctgaaac tgtaatgcta
      121 gagtgcagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgttgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012531
VERSION     KHUT01012531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           87 g           56 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcgggcgat
       61 ttagtcagag gtgaaatccc aaggctcaac cttggaactg cctttgatac tgattgtctt
      121 gagttcgaga gaggtaagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggc tcgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012532
VERSION     KHUT01012532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c          100 g           40 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcgggccgt
       61 caagtggtgt atgaaagcac tgtgctcaac gcagtgacgg taggccagac tgacggtctg
      121 gagggcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcacctga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012533
VERSION     KHUT01012533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggccaga
       61 aaagtttgat gtgaaatctc cgggcttaac ccggaaaatg cattgaatac tgtctggctt
      121 gaggattgga ggggagactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012534
VERSION     KHUT01012534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           81 g           56 t
ORIGIN      
        1 tacgagtgct ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtctttc gttaaattct tcggcttaac cgggggcatg cgggagaaac ggcacaacta
      121 gaggatgcga gaggtatatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca atatactggc gcactcctga cactgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01012535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012535
VERSION     KHUT01012535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggtggtttat
       61 caagtttggt gtgaaatccc tgggcttaac cgaggaaggg catcaaaaac gggtagactc
      121 gagtaagaga gagggctgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagc ggcgaaggcg gcggcctggc tcttaactga cactgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012536
VERSION     KHUT01012536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           92 g           53 t
ORIGIN      
        1 tacgtaggca gcaaacgttg ttcggagtta ctgggcgtaa agagtacgta ggcggtttgc
       61 caagtttggt gtgaaatctc ctggcttaac tgggagggtg cgccgaatac tggcaggctc
      121 gagtgttgga gaggggagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctccctgga caaccactga cgctgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012537
VERSION     KHUT01012537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           85 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgca ggtggttggg
       61 taagttagat gtgaaatccc cgggcttaac ctgggaactg cgtttaaaac tgctcgacta
      121 gagtattgca gagggtagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actacctggg caaatactga cactgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012538
VERSION     KHUT01012538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatttg
       61 taagtacctt gtgaaatccc atggctcaac catggaactg ctcggtatac tgcgaatctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012539
VERSION     KHUT01012539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           83 g           56 t
ORIGIN      
        1 tacgtagacc tcgagcgtta tccggattta ctgggcgtaa agcgtgcgta ggtgttttcg
       61 caagtctcta gtgaaatctc cgggcttaac tcggaaactg ttagggaaac tgcgagaatt
      121 gagtatggca gaggcagatg gaatggttgg tgtagcggta aaatgcgttg atatcaacca
      181 gaacaccagt ggcgaaggcg atctgctggg ccagtactga cactgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012540             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012540
VERSION     KHUT01012540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           93 g           46 t
ORIGIN      
        1 cacgtaggca ccgagcgttg tccggattta ttgggcgtaa agagcttgta ggcggttcgg
       61 taagtcgggt gtgaaacctc tgggctcaac tcagagacgc cacccgatac tgctgtgact
      121 cgagtgcggt aggggagcgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag tggcgaaggc gccgctctgg gccgtaactg acgctgagaa gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012541
VERSION     KHUT01012541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 caagtcaggg gtgaaatccc ccagcttaac tggggaagtg cctttgatac tgccgatctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012542
VERSION     KHUT01012542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           92 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 taagtcggaa gttaaaacgg gaggctcaac ctcttgtcgc tttcgatact gccatggctt
      121 gagtttggta ggggatgacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gtcatctggg ccaatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012543             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012543
VERSION     KHUT01012543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c          101 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgg
       61 cacgtccgtc gctgaaatcc cccggctcaa ccggggaagg ggcggggata cgaccgggct
      121 ggaggggggt aggggaatcg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gcggttctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012544             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012544
VERSION     KHUT01012544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggattca ctgggcgtaa agggtcgtgt aggtggtttt
       61 ccgcatcgtg tttgaaagcc cgaggcttaa cctcggcgtt ggacacgaga tggggagact
      121 cgagggaggc agaggaacgg ggaactcata gtggagcagt gaaatgcgtt gatattatga
      181 ggaacaccaa aggcgaaggc accgttctgg gcctctcctg acactgagac acgaaagcta
      241 ggggagcgaa gcag
//
LOCUS       KHUT01012545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012545
VERSION     KHUT01012545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcttg
       61 taagtcagtg gtgaaatctc cgggctcaac tcggaaactg ccattgatac tataagtctt
      121 gaattatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gcttactacg ggtatattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012546             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012546
VERSION     KHUT01012546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           99 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 taagtcggat gtgaaatctc caggctcaac ttggagcgtg catccgatac tgtcatggct
      121 agagtccggt agggggccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggcctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012547
VERSION     KHUT01012547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcgggccgg
       61 aaagtctgat gtgaaatccc ggggcttaac tccggaactg cattggatac tgtcggcctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012548
VERSION     KHUT01012548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           82 g           52 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtctga
       61 taagtctatt gtgtaagctc ctggcttaac tgggagaggc caatagaaac tatcaggctt
      121 gagtatagga gaggatactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtatctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012549
VERSION     KHUT01012549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgtgcgta ggcggccctc
       61 caagttgaat gtgaaagccc tgagctcaac tcaggaagtg cattcaagac tggtgggctt
      121 gagtcctgga gagggtggta gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaataccagt ggcgaaggcg accacctgga cggagactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012550             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012550
VERSION     KHUT01012550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           64 c          111 g           31 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattca ttgggcgtaa agggcgcgta ggcggcccgc
       61 agcagtggcg cgtggaatcc cccggctcaa ccgggggggg ccgcgccaga cgggcgggct
      121 cgagctgggt agagggtcgc ggaacgggcg gagtaggggt gaaatccgta gagaccgtcc
      181 ggaacaccgt tagcgaaggc ggcgacctgg gctcatgctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cgga
//
LOCUS       KHUT01012551             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012551
VERSION     KHUT01012551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctgag
       61 taagtcgggt gtgaaacctc caggctcaac ctggagccgc cactcgatac tgctctggct
      121 tgagtccggt aggggcccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgggctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012552
VERSION     KHUT01012552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtttgt
       61 taagtcggct gtgaaagccc gaggctcaac ctcggagagt cggccgatac tggcaagcta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg ggctactggg ccgatactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012553
VERSION     KHUT01012553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 gacgagggac cctagcgttg ttcggaatca ttgggcgtaa agcgggtgca ggtggctttg
       61 taagtcaggt gtgaaatccc agggcttaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgctgga gaggctacta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccgga ggcgaaggcg ggtagctggc cagacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012554
VERSION     KHUT01012554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgtta ctcggaatta ctgggcgtaa agcgcgcgta ggcgggtttg
       61 taagtcgatt gtgaaagccc tgggctcaac ccgggaattg cagtcgaaac tgcaaatctt
      121 gagtgcctga gaggatggcg gaattcccgg tgtagaagtg aaattcgtag atatcgggag
      181 gaacacctga ggcgaaggcg gctatctggc ggtgcactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012555
VERSION     KHUT01012555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           88 g           46 t
ORIGIN      
        1 gacagaggcc acaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggctcacc cgtggaactg cttggtatac tgggcgtctt
      121 gagccatcta ggggcagctg gaacgaacgg tggagcggtg aaatgcgtag atatcgttcg
      181 gaacgccaat ggtgaaaaca ggctgctggg gatgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012556
VERSION     KHUT01012556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           54 c           87 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 caagtccgcc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatttccgg tggagcggtg aaatgcattg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01012557             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012557
VERSION     KHUT01012557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           83 g           51 t
ORIGIN      
        1 caccagcgcc tcgagtgggg gtcgcgatta ttgggtctaa agcgtccgta gcaggctttg
       61 taaatgcttt gtgaaattgt ccggcataac cggacagcgt gcagagtaca ctgcagagat
      121 tgagaccggg agaggttagc agtattcctt gggtaccggt aaaatgggtt aatccaagga
      181 ggactaccaa tggcgaaggc agctaaccag aacggttctg actgtgaggg acgaaagcta
      241 ggggagcaaa acgg
//
LOCUS       KHUT01012558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012558
VERSION     KHUT01012558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c          100 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggtggtttgt
       61 cgcgttgttc gtgaaaaccg gaggctcaac ctctggcgtg cgggcgatac gggcagactt
      121 gagtactgca ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012559
VERSION     KHUT01012559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           82 g           52 t
ORIGIN      
        1 tacgagtacc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggtcttg
       61 ttagtctcct gtcaaaactt ctggctcaac cagaagattg caggggaaac ggcaaggctt
      121 gagagtgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcatttctga cgctgaaaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012560
VERSION     KHUT01012560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          103 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtc
       61 cgagtcaggg gtgaaagccc ccggcccaac cggggaagtg cccctgatac cgggcgtctg
      121 gagggaggta ggggtcgccg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ggcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012561
VERSION     KHUT01012561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           50 c           79 g           48 t
ORIGIN      
        1 tacagaggat gcaagcgtta tccgaaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 taagtccgcc gtcaaatccc agggctcaac tctggacagg cgatggaaac taccaagctt
      121 aagtacggta ggggtagagg gaatttttgg tggagcggtg aaatgcgtag agatcgaaaa
      181 gaacaccaac gacgaaagca ctctgctggg ccgacactga cactgagaga ccaaagctag
      241 gggagcgaat gag
//
LOCUS       KHUT01012562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012562
VERSION     KHUT01012562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgcgtgta ggcggtctgt
       61 taagtcggat gtgaaatctc tgggcttaac ccagaaatcg catccgatac tggcagactt
      121 gaggacggta gaggtaggtg gaattcccgg tgtaccggtg aaatggacag atatcgggag
      181 gaacaccagt ggcgaaggcg gcctactggg ccgatcctga cgctgagacg cgaaagccag
      241 gggagcgaac cgg
//
LOCUS       KHUT01012563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012563
VERSION     KHUT01012563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggtcaga
       61 caggtcggat gtgaaatccc tcggctcaac caaggaagtg cattcgaaac cgtctgactc
      121 gagtcccgga gaggaaggtg gaattctcgg tgtagaggtg aaattcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gccttctgga cgaggactga cgctgagatg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012564
VERSION     KHUT01012564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggg
       61 caagtcggag gtgaaagccc acagctcaac tgtggaagtg ccttcgatac tgccggtcta
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tctctactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012565
VERSION     KHUT01012565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           40 c           94 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtgggctgt
       61 taagtctggt ttgaaagttg gtcgcttaac gatcaagggt ggctggatac tggcggtctt
      121 gaatggggtg gaggtagccg gaatgggttg tgtagcggtg aaatgcatag agatgacccg
      181 gaacaccgat tgcgaaggca ggctactacg ccttaattga cactgaggca cgagagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012566
VERSION     KHUT01012566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatccc acggctcaac cgtggaactg cctcggatac tgacggcctc
      121 gaggggggta ggggcatgcg gaactgtagg tggagcggtg aaatgcgttg atatctacag
      181 gaactccggt ggcgaaggcg gcatgctgga ccttcactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012567
VERSION     KHUT01012567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           98 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggacccc
       61 caagtccggg gtgaaatact tcggctcaac cgaagaattg cctgggatac tggaggtctc
      121 gagggaggta ggggccgacg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gtcggctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012568
VERSION     KHUT01012568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           83 g           52 t
ORIGIN      
        1 tacgaaggga gctagcgtta ctcggaataa ctgggcgtaa agcgcatgta ggcggaaata
       61 caagtcagag gtgaaagccc tgggctcaac ccaggaattg cctttgaaac tgtatttctt
      121 gagtatgaca gaggatggtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg accatctggg tcataactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012569
VERSION     KHUT01012569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           98 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 atagccaagg gtgaaatcct ccggctcaac cggagaaggg cccttggtac tggcagcctc
      121 gaggggtgca ggggcgtgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacaccggt ggcgaaggcg gcacgctgga cacctgctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012570
VERSION     KHUT01012570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggccacg
       61 taagtcagat gtgaaatccc agggcttaac cctggaagtg catctgatac tgcgcggctt
      121 gagtcacgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cgatgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012571             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012571
VERSION     KHUT01012571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttcgg
       61 taagtcgggt gtgaaatctc cgtgctcaac acggagcggc cacctgatac tgccgtgact
      121 cgagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcactctgg gccgtgactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012572
VERSION     KHUT01012572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgctcgta ggcgggtgtt
       61 taagtcgatc gtgaaagccc tgggcttaac ctgggaattg cggtcgatac tgagcaccta
      121 gagtatggta gaggatagtg gaattcccgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actatctgga tcaatactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012573
VERSION     KHUT01012573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtcata
       61 agagtgtgat gtgaaagccc cgggcttaac ctgggaagcg catcgcaaac gctatgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaagcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012574             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012574
VERSION     KHUT01012574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ttgggcttaa agagcgcgta ggcggcgagg
       61 caagtgaggg gtgaaatcct atcggctcaa ccgtggaata gcctttcata ctgcctcgct
      121 cgaggaaggt aggggtcagt ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccag tggcgaaagc gactgactgg atcttgtctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012575
VERSION     KHUT01012575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           86 g           48 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ttcggaatca ctgggcgtaa agcgggtgta ggtggctctg
       61 caagtcacat gtgaaagccc cgggctcaac ccgggaagtg catgtgatac tacagagctt
      121 gagtaaggga gaggatggca gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaactccggt ggcgaaggcg gccatctggc ccattactga cactcagacc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012576             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012576
VERSION     KHUT01012576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           97 g           46 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattca ctgggcgtaa agggcgcgta ggcggttacg
       61 caagtcggat gtgaaaagtc acggctcaac tgtgatcgtg catccgatac tgcgttgact
      121 tgaggtcggt agaggcaagt ggaactagcg gtgtagcggt gaaatgcgca gatatcgcta
      181 ggaacgccgg tggcgaaggc gacttgctgg gccgtacctg acgctgaggc gcgaaagcta
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01012577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012577
VERSION     KHUT01012577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           80 g           53 t
ORIGIN      
        1 tacgtagggc ccaagcatta tccggaatta ctgggcgtaa agagtgcgta ggtgttcctt
       61 caagtcggtc gttaaatctt agagcttaac tctaaggccg cgagcgatac tgaaggaatt
      121 gagtactgca ggggcaagca gaatttttgg agtaggggtg aaatccgttg ataccaaaag
      181 gaataccaat ggcgaaggca gcttgctagg cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01012578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012578
VERSION     KHUT01012578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           92 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaagtg cctttgatac tggcgagctc
      121 gagtgaggta ggggcagatg gaactgttgg tggagcggtg aaatgtgttg atatcaacag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012579
VERSION     KHUT01012579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           99 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggggaag
       61 cgcgtcggac gtgaaaggct ggggctcaac cccagacagc cgttcgaaac ggcttctctt
      121 gaggaccgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012580             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012580
VERSION     KHUT01012580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           86 g           49 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtacgta ggcggcacgt
       61 taagtgagat gcgaaatcct gtggctcaac catatggact gtatctcata ctggcgcgct
      121 tgaggtcggc agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gatatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccgcacctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012581
VERSION     KHUT01012581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           96 g           44 t
ORIGIN      
        1 gacagaggtc tcgagcgtta atcggattta ttgggcttaa agcgtgcgta ggcgggccgc
       61 taagcgtctt gtgaaagccc ccggctcaac cggggaactg cgggacggac tggcggcctt
      121 gaggcaggta tgggcggaca gaactgtcgg tggagcggtg aaatgcgtag agatcgacag
      181 gaatgcctat ggtgaagacg gtccgctggg cctgtcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012582
VERSION     KHUT01012582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c           98 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 ttagtctggg gtgaaatctt tcggcttaac cggaaaagtg cctcggatac tggcagtctt
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcctgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012583
VERSION     KHUT01012583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           92 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggccaac
       61 taagtcagac gtgaaatccc taggctcaac ctaggaactg cgtctgatac tggatggctt
      121 ggatccggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccaatattga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012584
VERSION     KHUT01012584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           93 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggactgg
       61 caagtctgat gtgaaaggcg ggggctcaac ccctggactg cattggaaac tgttagtctt
      121 gagtgccgga gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cggtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012585
VERSION     KHUT01012585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcctc
       61 caagtcagtg gtgaaagccc ggagctcaac tccggaattg ccattgaaac tgtgggactt
      121 gagtacgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcgttactga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012586
VERSION     KHUT01012586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agcgtgagta ggtggctcgc
       61 taagtcggtt gtgaaatccc tgggctcaac ctgggaattg cagtcgatac tggcaagcta
      121 gagttcggta gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcgat ggcgaaggca accacctggg cctgaactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012587
VERSION     KHUT01012587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           94 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttag
       61 taagtcagat gtgaaagccc ggggcttaac cccggaagtg cgtttgaaac tactagacta
      121 gagtgcagta gaggttggtg gaattcccag tggagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctgtaactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012588
VERSION     KHUT01012588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           93 g           48 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgaga
       61 tgtgagtgat gtgaaagccc tgggcttaac ctaggaagcg catcgctaac tatctgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012589
VERSION     KHUT01012589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           87 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggctta
       61 taagtcgatt gtgaaatccc tgggcttaac ctgggaactg cagtcgaaac tgtaagcctt
      121 gaatgtctga gaggatggcg gaataaccgg tgtagtagtg aaatacgtag atatcggtta
      181 gaacacctga ggcgaaggcg gctatctggc ggaacattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012590
VERSION     KHUT01012590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta gacggcttaa
       61 taagtcaggt gtgaaagacc cgggctcaac ctgggggcgg catctgagac tgttgagctt
      121 gagtgcagga gaggcaagtg gaattcccag tgtagcagtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg acttgctgga ctgtaactga cgttgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012591
VERSION     KHUT01012591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           81 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcatgta ggtggttgga
       61 taagtctgtg gtgaaatttc tcagctcaac tgggaaactg ccacggaaac tatctgactt
      121 gagtgtggga gggggagacg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaat ggcgaaagca gtctcctggc ccaatactga cactgagatg cgaaagctag
      241 ggtagcaaac agg
//
LOCUS       KHUT01012592             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012592
VERSION     KHUT01012592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           91 g           53 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcaggt gtgaaaactc taggctcaac ttagagaggc cacttgatac tgctatgact
      121 tgagttcggt aggggaccat ggaattcctg gtgtagcggt ggaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggtctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012593
VERSION     KHUT01012593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggactga
       61 caagtcagac gtgaaatccc cgggctcaac ccgggaactg cgcttgaaac tgtcagtctt
      121 gagtgttgga gaggatcgtg gaattcctgg tgtacaggtg aaattggtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgatctgga caacaactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012594
VERSION     KHUT01012594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgtgca ggtggcttgg
       61 taagttaggc gtgaaatctc ccggctcaac tgggataggt cgtctaatac taccgggcta
      121 gagaacagaa gaggaagatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcttctggt ctgtttctga cactcagacg cgacagctag
      241 ggtagtaaac ggg
//
LOCUS       KHUT01012595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012595
VERSION     KHUT01012595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           85 g           52 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggagttc
       61 taagtacctt gtgaaatccc accgctcaac ggtggaactg cttggtatac tggttctctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012596
VERSION     KHUT01012596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           91 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggtgcgta ggtggttaat
       61 taagttagct gtgaaagccc cgggcttaac ctgggaattg cagctaaaac tgaatagcta
      121 gagtagggga gaggagtgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcactctgga accatactga cactgaggca cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012597
VERSION     KHUT01012597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           83 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggcggattgg
       61 taagtcagtg gtgaaagccc cgagctcaac ttgggaattg ccattgatac tgctgatctt
      121 gagtacggtt gaagtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcactaag ccgttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012598
VERSION     KHUT01012598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           91 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 taagtcagct gtgaaatccc cgggctcaac ctgggaatgg cagttgagac tggcaggctg
      121 gagtgtgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012599
VERSION     KHUT01012599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           93 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttatgt gtgaaagacc agggcttaac cttggggtgt cagataagac tgttagactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012600
VERSION     KHUT01012600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggttgtctca
       61 taagtccact gtgaaatccc cgggcttaac ccgggaagtg cggtggatac tgtaagacta
      121 gagtgctata ggggtacgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgtactgga gagcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012601
VERSION     KHUT01012601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggcgcag
       61 caagttcgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tgctgtgctt
      121 gaggcaagga ggggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc cttagcctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012602
VERSION     KHUT01012602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           82 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggcccgg
       61 taagtcagtg gtgaaatccc tcagcttaac tgaggaattg ccattgaaac tgccaggctt
      121 gaatacagtt gaagtgggcg gaatacaaca tgtagcggtg aaatgcttag atatgttgta
      181 gaacaccgat tgcgaaggca gctcactaag ctgtgattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012603
VERSION     KHUT01012603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           82 g           57 t
ORIGIN      
        1 gacggagggt gcaagcgtta tccggattta ctgggtttaa agggtacgta ggcggcctga
       61 taagtcagtg gtgaaaacta gcagctcaac tgctaacgtg ccattgatac tgccaggctt
      121 gagtatagta gaggtgggtg gaattctaca tgtagcggtg aaatgcttag atatgtagag
      181 gaacgccgat aacgaaggta actcactggg ctattactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012604
VERSION     KHUT01012604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           81 g           56 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggtaaag
       61 aaagtcggat gtgaaatccc ggggcttaac ctcggacccg cattcgaaac ttcttttcta
      121 gagggtagat ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacatctgt ggcgaaggcg gttttctaat ttacacctga cgctgaggcg cgaaagtaag
      241 gggatcaaac agg
//
LOCUS       KHUT01012605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012605
VERSION     KHUT01012605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtgcat
       61 caagtcagag gtgaaagccc ggagctcaac ttcggaactg cctttgaaac tagtgcacta
      121 gaatcttgga gaggcgggtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gcccgctgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012606
VERSION     KHUT01012606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcggtcgtg
       61 tgcgtcgagg gtgaaatccc caggcttaac ctgggagctg ccttcgatac ggcacggctt
      121 gagtccggga gaggggagca gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaataccagt ggcgaaggcg gctccctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012607
VERSION     KHUT01012607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggttacg
       61 cgagtcgggt gtgaaagccc ggggctcaac cccggacgtg cactcgatac tacgtagctg
      121 gagtactgta gaggaaggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012608             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012608
VERSION     KHUT01012608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           79 g           55 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggaatta ctgggcgtaa agcgtgcgca gacggtttga
       61 taggtctaat gcgaaatcca gtggctcaac catttggact gtattagaaa ccatctgact
      121 agaggtaggt agaggcaagt ggaatttctg gtgtaggggt gacatccgta gatatcagaa
      181 ggaacaccaa tggcgaaggc agcttgctgg gccttacctg acgttcaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012609             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012609
VERSION     KHUT01012609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           47 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 caagtcgggt gtgaaacccc caggcttaac ctggggacgc catccgatac tgctatggct
      121 tgagtccggt aggggcgcat ggaatacccg gtgtagcggt gaaatgctta gatatcgggt
      181 ggaacaccag tagcgaaggc ggtgcgctgg gccggaactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012610
VERSION     KHUT01012610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           87 g           48 t
ORIGIN      
        1 gacgagcctc ccgaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagtagggt gtgaaatccc tcggctcaac cgaggaactg cgctctaaac taccgtgctc
      121 gagggagata gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012611
VERSION     KHUT01012611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           82 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggagtta ctgggcgtaa agagctcgta ggtggttctt
       61 caagtccgat gtgaaatccc tgaactcaat tcaggaagtg catcggatac tgctgaacta
      121 gagtatagga gaggaaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagc ggcgaaggcg gctttctgga ctataactga cactgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012612
VERSION     KHUT01012612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           84 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggaaaaa
       61 taagtctaat gtgaaagccc tcggcttaac cgaggaactg catcggaaac tgtttttctt
      121 gagtgcagaa gaggagagtg gaactccatg tgtagcggtg gaatgcgtag atatatggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ctgcaactga cgctgaggct cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012613
VERSION     KHUT01012613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           96 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtaagg
       61 agggtgtgat gtgaaagccc tgggcttaac ctgggaagtg catcgcaaac tacctgactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012614
VERSION     KHUT01012614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggctttc
       61 taagttggat gtgaaaactc tgggctcaac ccagagactg catccaaaac tggagagctt
      121 gggttttgga ggggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga caaataccga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012615
VERSION     KHUT01012615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcat
       61 caagtccggg gtgaaatctt ccggcttaac cggaaaagag ccttggatac tggtgatctg
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaggcg agctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012616
VERSION     KHUT01012616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           89 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgtgagta ggtggtctca
       61 tttgtcctgg gtgaaatccc cgagcttaac ttgggaactg ccctggaaac ggtgagacta
      121 gagttctgga gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaagcg gcgacctgga cagacactga cactcaatca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012617
VERSION     KHUT01012617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           83 g           57 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgcgta gacggcatat
       61 taagtctgtt gttaaatctt ccggcctaac cggaaatctg cagtagatac tgatgtgcta
      121 gagggtgaaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgaggg
      181 gaacaccaat ggcgaaggca gcttcttggt tcacatctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012618
VERSION     KHUT01012618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggtggtttgt
       61 tgcgtcggct gtgaaaactt ggggctcaac cccaagcccg cagccgatac gggcagacta
      121 gaggtaggta ggggaaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctttctggg ccttacctga cactgaggag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012619
VERSION     KHUT01012619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           92 g           47 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggatggc
       61 taagtgaggt atgaaatccc ggtgcttaac agcgggatgg tacctcagac tagtgatctg
      121 gagtctgaga gagggatgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaagcg gcatcctggc tcagcactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012620
VERSION     KHUT01012620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           43 c           76 g           55 t
ORIGIN      
        1 gacgtaggat gcaaacgtta tccggaatta ttgggcgtaa agagttcgta ggtggtttgt
       61 taagtagagc gtcaaagact tgagcctaac tcaaggaagg tgctctatac tgactcacta
      121 gagtaagaca ggggcaggtg gaattctgta tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gcctgctggg tctttactga cactcaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012621
VERSION     KHUT01012621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatgtc
       61 taagtacctt gtgaaatccc accgctcaac ggtggaactg cttggtatac tggatgtctt
      121 gaggtgctca ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaat ggtgaagaca gctcgctggg ggcatactga cgctgagacg cgaaagccag
      241 gggatcaaag ggg
//
LOCUS       KHUT01012622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012622
VERSION     KHUT01012622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           79 g           54 t
ORIGIN      
        1 tacagagacc ccgagcgtta tccggaatta ctgggcgtaa aggacgagta gtcggttatg
       61 atagtctgat gttaaatctc atcgctcaac ggtgaggccg cattagaaac gtcatttcta
      121 gagcgagtca ggggcaagcg gaacgtgtgg tgtagcggtg aaatgcattg atatcacacg
      181 gaacaccaaa agcgaaggca gcttgctggg gctttgctga cgatgaatcg tgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01012623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012623
VERSION     KHUT01012623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           61 c           77 g           49 t
ORIGIN      
        1 tccccgcagt ccaagtcgca accacatttg ttgggtctaa aacatccgta gcttgctttg
       61 taagtctcgt gtgaaatctc gtctctcaag ggcgaacgtg cacgagatac tgcaaagcta
      121 gagaccggga gacgcagtga gtacgcatcg ggtaccggta aaatgggttg atccaatgcg
      181 gactaccaac cgcgaaggca cgctgcgagc acggatctga cagtgaggga tgaaggctag
      241 gggcgcaaag tgg
//
LOCUS       KHUT01012624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012624
VERSION     KHUT01012624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c           97 g           37 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggcggtgccg
       61 cacgtcggat gtgaaagccc ggggctcaac cccggaagcg catctgaaac gacggcgctg
      121 gagggtgaga gaggcgagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actcgctggc tcactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012625
VERSION     KHUT01012625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacgaggggc tcaagcgttg tccggagtta ttgggcgtaa agggtgcgta ggaggttgtg
       61 ttagtcgatg tttaaagcct tgggcccaac ccaagataag gcatcgaaac ggcacaactc
      121 gagggtacga gaggtgagcg gaactcatag tggaggggtg aaatccgttg atattatggg
      181 gaacaccaaa ggcgaaggca gctcactggc gtatacctga ctctgaggca cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01012626             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012626
VERSION     KHUT01012626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           89 a           28 c           67 g           70 t
ORIGIN      
        1 tacgggagga gcgagcatta ttcgggatga ttaggcgtaa agagtttgta ggttgttttt
       61 taagttgaaa gaaacaaatt aaagcccaac tttatacatt ttttcaaaac tggagaactt
      121 gagtataaat agaggatgat ggaatttcta ttggagtgat aaaatacgtt gataatagaa
      181 ggaagaccta tggcgaaggc aattatctgg gtatatactg acactgagaa acgaaagctt
      241 gggtagcgaa cggg
//
LOCUS       KHUT01012627             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012627
VERSION     KHUT01012627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           81 g           55 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggtttag
       61 gcaagtcagg tgtaaaaacc ccgggcttaa cctgggaatg tcgcttgaga ctgcctagct
      121 agagtactat agaggatagt ggaatttcca gtgtagcggt gaaatgcgta gatattggaa
      181 ggaacaccag tggcgaaggc gactatctgg atagaaactg acactgaaac acgaaagcat
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012628             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012628
VERSION     KHUT01012628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           63 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggcctc
       61 tgcgtcggtc gctgaaagcc cccggctcaa ccggggaagc ggcgccgaaa cgaggggcct
      121 ggaggggggt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg accctttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012629
VERSION     KHUT01012629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtcaggg gtgaaatctt tcggcttaac cggaaaacag cctctgatac tggcagtctg
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttctctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012630
VERSION     KHUT01012630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttact
       61 taagtctggt gttaaatact gaagctcaac ttcagcatgg cattggaaac tgggtggcta
      121 gagtgcggta gaggtctcgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg cgagactggg ccgtaactga cgctgaggaa cgaaagccag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01012631             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012631
VERSION     KHUT01012631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           97 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggcctgt
       61 taagtttgct gtgaaatctc ccggctcaac tgggagggtg cggtgaatac tgacgggctt
      121 ggagtgcggg agaggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggtgtagac ggctctctgg accgtaactg acgctgagac acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012632
VERSION     KHUT01012632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 aaagtcggac gtgaaagctc ctggctcaac tgggagaggc cgttcgaaac taccaggcta
      121 gagggcagta gagggaggtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcctcctgga ctgtccctga cgctcagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012633
VERSION     KHUT01012633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           51 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggctgttaat
       61 caagttaggt gtgaaagccc tgggcttaac ctgggaactg catttaatac tgattgactg
      121 gagagcgaga gaggaaagcg gaattcctag tgtagaagtg aaatctgtag atattaggag
      181 gaacaccaga ggcgaaggcg gctttctggc tcgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012634
VERSION     KHUT01012634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           84 g           52 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agagcatgta ggcggcttag
       61 taagtcagtt atcaaagacc gatgcttaac atcgggccgg tatctgatac tgctgggcta
      121 gaggatagca ggggtacatg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca atgtactggg ctatatctga cgctgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012635             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012635
VERSION     KHUT01012635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           62 c           98 g           36 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccc
       61 taagcgccat cgtgaaatcc cgccgctcaa cggcggaacc gcgtggcgga ctgggggtct
      121 tgaggggctc aggggtcacc ggaacaggcg gtggagcggt gaaatgcgta gatatcgcct
      181 ggaacgccga cggtgaaaac aggtgactgg gagccttctg acgctgagac acgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012636             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012636
VERSION     KHUT01012636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           44 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 taagttgggt gtgaaattct cgggctcaac ccgggaacgc cacccgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012637
VERSION     KHUT01012637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           84 g           55 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggtggctttt
       61 caagtcagag gtgaaatccc caggctcaac ctgggaattg cctttgaaac tgttaagctt
      121 gagtactgga ggggatggtg gaatttctaa tgtagcggtg aaatgcgtag atattagaag
      181 gaacaccggt ggcgaaggcg accatctgga cagtaactga cactgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012638
VERSION     KHUT01012638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggacag
       61 caagtccggg gtgaaagctt tcggctcaac cggaaaagtg cctcggatac tgttgtcctc
      121 gagggaggta ggggtcggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg accgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012639
VERSION     KHUT01012639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           37 c           77 g           54 t
ORIGIN      
        1 tacgtgagtg gcaagcgtta tccggaatta ctgggcgtaa agggcgtgta ggtggtacta
       61 taagtcatag gtaaaaacac tgagcttaac tcagatggag ccaatgaaac tgtagaacta
      121 gagtatagga taggaagatg gaattattac tgtagcggtg gaatgcgtag atagtaatag
      181 gaaggccaac gacgaaggta atcttctgga ctaatactga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012640
VERSION     KHUT01012640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggacctt
       61 caagtcaggg gtgaaatcct ccagctcaac tggagaactg cctttgatac tggaggtctc
      121 gagtaaggta ggggtgtgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcacactgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012641
VERSION     KHUT01012641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgcgtgta ggtggccttg
       61 caagtcgggt gtgaaatccc tgggcttaac ccaggacgtg catccgaaac tgcttggctt
      121 gagtgccgga gagggcggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccgcctgga cggctactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012642
VERSION     KHUT01012642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           85 g           54 t
ORIGIN      
        1 tacagagggt gcaagcattg ttcggaatca ttgggcgtaa agggcgtgta ggcggttcgg
       61 taagtcagat gtgaaatccc tgggcttaac ccaggaactg catttgatac tgcttaactt
      121 gagtgcggtg gaggatagcg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctatctata ccgttactga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012643
VERSION     KHUT01012643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgagtgta ggcggctcta
       61 aaagtcaggt gtgaaatccc acggctcaac cgtggaactg catttgaaac tgtggagctt
      121 gagtattgca gaggatggta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg gccatctggg caaatactga cgctgagact cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01012644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012644
VERSION     KHUT01012644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           95 g           46 t
ORIGIN      
        1 cacgtagggg cctagcgttg tccggattta ttgggcgtaa agggcgcgta ggcggcctgg
       61 cgcgtctcct gcgaaagctc ccggcttaac cgggagaggt caggggaaac ggccaggctt
      121 gaggatagca gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaagcg accttctggg ctatttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01012645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012645
VERSION     KHUT01012645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c          100 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggtggaa
       61 taagtggtag gtgaaagccc cgggctcaac ctgggaagtg cctatcaaac tgtttgactg
      121 gagtgcagga gagggcagta gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaatgccggt ggcgaaggcg actgcctggc ctgacactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012646
VERSION     KHUT01012646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c          100 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caggtcgggg gtgaaatgcc gcagcttaac tgtggaagtg cccccgatac cggcggcctc
      121 gagggggata ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acacgctgga tccctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012647
VERSION     KHUT01012647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtttac
       61 taagtctggt gtgaaagccc gaggcttaac ctcggaagtg cactggatac tggtaagcta
      121 gaggacgaga gagggaagtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcggt ggcgaaggcg acttcctggc tcgtaactga cgctcagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012648
VERSION     KHUT01012648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatta ttgggcgtaa agggcgtgta ggcggccttg
       61 tatgtctcat gtgaaagccc tcggctcaac cggggaactg catgggaaac ggcaaggctt
      121 gagtccggga gaggatagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctatctgga ccggtactga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012649
VERSION     KHUT01012649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           84 g           57 t
ORIGIN      
        1 tacgtagggt gctaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggttgccaag
       61 taagttgaat gtgaaagccc tcggcttaac cgaggaagtg cattcaaaac tgctcggcta
      121 gaggatggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaatacctgt ggcgaaggcg gccttctggg ccattcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012650
VERSION     KHUT01012650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttcg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcgaggctt
      121 gagtaccgga gaggatggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccatctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012651
VERSION     KHUT01012651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           99 g           43 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggggcga
       61 caagtcgagt gtgaaatccc tcggctcaac cgaggaactg cgcccgaaac tgtcgctctt
      121 gagtcccgga gaggaagatg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gtcttctgga cggtgactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012652
VERSION     KHUT01012652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           46 c           98 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggtgtaa agggtgtgta ggcgggtcgg
       61 taagtcaggg gtgaaaggca gcggcttaac cgttgaattg cctttgatac tgccggtctt
      121 gagtcccgga gaggtcactg gaattcgtgg tgtagcggtg aaatgcgtag atatcacgag
      181 gaacaccgga ggtgtagacg ggtgactgga cggcgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012653
VERSION     KHUT01012653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           93 g           51 t
ORIGIN      
        1 tacagggggt gcgagcgtta ttcggaatta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagtttggt gtgaaagccc cgggctcaat ccgggaagtg cattgaatac tgccagacta
      121 gagtgcagga gaggagagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gctctctgga ctgttactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012654
VERSION     KHUT01012654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           85 g           49 t
ORIGIN      
        1 gacgaacctc ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgcgtag
       61 taagtcgggt gtgaaatccc ccagcttaac tgaggaattg cgcccgatac tgctgcgctg
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012655
VERSION     KHUT01012655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           97 g           47 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtattc
       61 caagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgggatgcta
      121 gagtgtggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacctgc ggtgtagacg gcttgctgga ccacgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012656
VERSION     KHUT01012656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           88 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcagat gtgaaatccc cgggctcaac cctggaactg catctgaaac tacctggcta
      121 gagtctcgga gaggaaggca gaattcccgg tgtaggggtg aaatccgtag ataccgggag
      181 gaataccagt agcgaaggcg gccttctgga cgaagactga cgctcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012657
VERSION     KHUT01012657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           51 c          101 g           48 t
ORIGIN      
        1 cacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggctcgt
       61 tgcgtcaacc gtgaaaaccc ggggcttaac ttcgggcttg cggttgatac gggcgggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012658
VERSION     KHUT01012658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacagaggcc ccgagcgtta atcggaatta ctgggcttaa agggtgcgta ggcggaccag
       61 aaagtgcttt gtgaaagccc ccggctcaac cggggaactg cggggcatac tgctggtctt
      121 gaggcaagta agggttgcga gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaatgccgat ggtgaagacg cgcaactggg cttgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012659
VERSION     KHUT01012659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaagcct ggggctcaac cccagaagtg catttgatac tgcgaagctt
      121 gagtgccgga gaggttatta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccgga ggcgaaggcg gataactggc cggacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012660
VERSION     KHUT01012660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcga
       61 caagttgggt gtgaaagccc agggctcaac cctggaattg cactcaatac tgtcttgctt
      121 gaattcggta gaggttggtg gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaacacccgt ggcgaaggcg gccaactgga ccgacattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012661             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012661
VERSION     KHUT01012661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           95 g           38 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcgggtcgt
       61 taagtgtccg cgtgaaatcc cacggctcaa ccgtggaacc gccgggcata ctggcgacct
      121 tgaggcacgc aggggcgagc ggaacaaatg gtggagcggt gaaatgcgta gatatcattt
      181 ggaacgccga cggtgaaaac agctcgctgg gcgtgaactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012662             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012662
VERSION     KHUT01012662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           87 g           56 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggattta ctgggcgtaa agcgtatcgt aggcggatgt
       61 gtgcgtggtg ggtttaagac ctcagctcaa ctgaggaact gctcgtcata cggcacgtct
      121 tgagtctgtt cgagggaact agaattctcg gtggaggggt gaaatccgta gatatcgaga
      181 ggaatgccaa gggtgaagac aggttcctag ggcagtactg acgctcatga acgacagcta
      241 ggggagcaaa aggg
//
LOCUS       KHUT01012663             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012663
VERSION     KHUT01012663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           81 g           56 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtacgta ggcgggtgtt
       61 taagtgaaat gcgaaatctc gtggctcaac catgtagact gtatttcata ctggacacct
      121 tgaggatggc agaggcaagt ggaattgata gtgtagcagt gaaatgcgta gatattatca
      181 ggaacaccaa tggcgaaggc agcttgctgg gccatatctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012664             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012664
VERSION     KHUT01012664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           80 g           53 t
ORIGIN      
        1 tacatagggc ccaagcgtta tccggaatta ttgggcgtaa agtgcgcgta ggcgttccga
       61 caagtcggtc gttaaaccat gtagcttaac tacatacatg cgagcgatac tatcggaatt
      121 gagttttgca ggggctagca gaatttctgg agtaggggtg aaatccgttg ataccagaag
      181 gaataccaat ggcgaaggca gctagctggg caataactga cgctgagagc gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012665
VERSION     KHUT01012665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggcccat
       61 taagtcgtat gtgaaatgcc acggctcaac cgtggaattg cgcacgatac tggtgggctt
      121 gaggtcggga ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg actcactggc ccgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012666
VERSION     KHUT01012666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           90 g           48 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgctcgtctt
      121 gagctgtcta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgat ggtgaaaaca ggttgctggg gacatgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012667             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012667
VERSION     KHUT01012667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           94 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agggctcgta ggtggtttgt
       61 cgcgtcgtct gtgaaattcc ggggcttaac tccgggcgtg caggcgatac gggcataact
      121 tgagtactgt aggggtaact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc aggttactgg gcagttactg acgctgagga gcgaaagcat
      241 gggtagcgaa cagg
//
LOCUS       KHUT01012668             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012668
VERSION     KHUT01012668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           92 g           51 t
ORIGIN      
        1 gacgaaccaa gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtctct
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaattg ccttggatac tgtggggctc
      121 gagggaggta ggggtcacgg gaacatccag tggagcggtg aaatgcgttg atattggatg
      181 gaacgccggt ggcgaaagcg cgtgactgga ccttttctga cgctgagtgc gcgaaagcca
      241 gggtagcgaa cggg
//
LOCUS       KHUT01012669             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012669
VERSION     KHUT01012669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           96 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcggat gtgaaatctc caggctcaac ctggaggggt catctgatac tgctatgact
      121 agaggtcggt aggggagtgt ggaattcctg gtggagcggt gaaatgcgca gatatcagga
      181 ggaacacccg tagcgaaggc ggcactctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012670             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012670
VERSION     KHUT01012670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           58 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ctgggcttaa agcgtgtgta ggcggaccgg
       61 cacgtccgat gttgaaatcc cccggcttaa ccggggaact ggcacggata cgaccggtct
      121 ggagggaggt aggggggtct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg accttttctg acgctgagac acgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012671             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012671
VERSION     KHUT01012671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           68 c           98 g           32 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cacgtcgact gctgaaatcc cccggctcaa ccggggaacg ggcatcgata cgaccggcct
      121 ggaggggggt aggggggacg ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg cggcgaaagc gcgtccctgg accccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012672             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012672
VERSION     KHUT01012672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           92 g           46 t
ORIGIN      
        1 tacagggggg gcaagcgtta tccggaatga ttgggcgtaa agggtctgta ggtggtttat
       61 gaagttcaat gttaaaaacc agggctcaac cctgggaagg cattgaagta ctcataaact
      121 agagtgcggt aggggtagag ggaactccgc gtggagcggt gaaatgcgta gagatgcgga
      181 ggaacaccag tggcgaaggc gctctactgg gccgagactg acactgagag acgaaagcta
      241 agggagcgaa tagg
//
LOCUS       KHUT01012673             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012673
VERSION     KHUT01012673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          108 g           39 t
ORIGIN      
        1 gacggagggg gcgagcgttg ttcggaatca ttgggcgtaa agggctcgta ggcgggcccg
       61 caggtctggc gtgaaatctc caccgctcaa cggggagggg tcgctggaaa ccgcgggtct
      121 ggagtagggt agagggtcgt ggaattccgg gtgtagcggt ggaatgcgta gatatccgga
      181 agaacaccgg tggcgaaggc ggcgacctgg gcccatactg acgctgagga gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012674             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012674
VERSION     KHUT01012674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaagta ggtggtctca
       61 tttgtctaca ggtgaaatcc ttgggcttaa ctcaagaagt gccttagaaa cggtgagact
      121 cgagtactgg agagggtcgt ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 ggaacaccag cggcgaaggc ggcgacctgg acagttactg acactcaact gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012675             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012675
VERSION     KHUT01012675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          101 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggttggg
       61 taggttggtt gtgaaaggtg cgggcttaac ccgcataggt caaccaaaac cgtccgactc
      121 tgaggacagt agaggaaggt ggaattccgg gtgtagtggt gaaatgcgta gagatccgga
      181 ggaacaccgg cggcgaaggc ggccttctgg gctggccctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa ctga
//
LOCUS       KHUT01012676             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012676
VERSION     KHUT01012676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           97 g           44 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttgg
       61 taagtcggat gtgaaacctc caggctcaac ctggagtcgc catccgatac tgccatggct
      121 agagtccggt aggggcccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgggctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012677
VERSION     KHUT01012677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          101 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcatg
       61 caagtccggg gtgaaagccc tcggctcaac cgaggaactg cctcggatac tgcgtgtctg
      121 gaggggggta ggggcggacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gtccgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012678
VERSION     KHUT01012678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           97 g           43 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcccgca ggcggcttgg
       61 caagtctgag gtgaaatctc ccggctcaac tgggagggtg ccccggaaac tgccaggctc
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012679
VERSION     KHUT01012679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           84 g           52 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgtacgta ggtggatatt
       61 taagtcgggt gtgaaagccc caggctcaac ctgggaactg cgtccgatac tgggtatcta
      121 gagtatggta gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cactgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012680
VERSION     KHUT01012680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           84 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttag
       61 taagttggtt gtgaaatccc tgggcttaac ctaggaattg cagtcaatac tgctaggcta
      121 gagtagaata gagggtagta gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga ttcatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012681
VERSION     KHUT01012681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatga ctaggcgtaa agggcaggta ggtggtcttg
       61 taagtctgct gtgaaagctc ccggcttaac tgggagaggt cagtggatac tgcgaggctt
      121 gagtatagga gagggtactg gaattcccgg tggagcggtg aaatgtgtag atatcgggag
      181 gaacaccaat ggcgaaagca agtacctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012682
VERSION     KHUT01012682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           46 t
ORIGIN      
        1 gacagaggtg gcgagcgttg ttcggaatta ctgggcttaa agggcgcgta ggcgtcccga
       61 caagtcaggt gtggaagctt cccgcttaac ggggaaattg catctgaaac tgtcgggatt
      121 gagtcagcga ggggatggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaaggccggt ggcgaaggcg gccatctggc gctgaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012683
VERSION     KHUT01012683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           86 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agagcgtgta ggcggttatg
       61 aaagtcagat gtgaaatccc cgggcttaac ccgggacgtg catttgaaac tccatagcta
      121 gagtgtggta gaggatagtg gaatacctag tgtagtggtg aaatacgtag atattaggta
      181 gaacaccggt ggcgaaggcg gctatctggg ccataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012684             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012684
VERSION     KHUT01012684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           75 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcata
       61 gtaagcaaat agtgaaatcg tgtggctcaa ccatatatcc attatttgaa ctgctaagct
      121 tgaagatgag agaggtagat ggaattccta gtgtaggggt aatatccgta gatattagga
      181 ggaacaccga tggcgtaggc agtctactgg ctcattcttg acactaaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012685             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012685
VERSION     KHUT01012685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 aaagtcaggt gtgaaaactc tgggcttaac ccagagacgc cacttgatac ttctatggct
      121 tgagttcggt aggggaccat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtggtctgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012686             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012686
VERSION     KHUT01012686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatta ttgggcttaa agagcgcgta ggcggcgagg
       61 caagtgaggg gtgaaatcct atcggctcaa ccgtggaata gcctttcata ctgccttgct
      121 cgaggggggt aggggtcgat ggaacttccg gtggagcggt gaaatgtgtt gagatcggaa
      181 ggaacgccag tggcgaaagc gatcgactgg atcttttctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012687             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012687
VERSION     KHUT01012687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           82 g           50 t
ORIGIN      
        1 tacgtagggt acaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcgggagtg
       61 aaagtcagat gtgaaattcc aaagctcaac tttggggctg catttgaaac tccacttctc
      121 agagggatgg cggagaaagc ggaattccac gtgtagcggt aaaatgcgta gatatgtgga
      181 agaacaccgg tggcgaaagc ggctttctag cttaaccctg acgctgaggc gcgaaagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012688             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012688
VERSION     KHUT01012688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           62 c          111 g           35 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggccacg
       61 ttggccacca cgtgaaagcc cccggctcaa ccgggtggtg gcgggtggga ccgcgtggct
      121 ggaggatggt aggggccagt ggaattcccg gtgtagtggt gaaatgcgta gataccggga
      181 ggaacacccg cggcgaaggc ggctggctgg gccattcctg acgctgaggc gcgaaggcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012689             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012689
VERSION     KHUT01012689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           48 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttga
       61 taagtcgggt gtgaaacctc caggctcaac ctggagcagc cactcgatac tgtcatggct
      121 tgagttcggc aggggaacac ggaattcctg gtgtagcggt gaaatgcaca gatatcagga
      181 ggaacaccga tggcgaaggc agtgttctgg gccgagactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012690             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012690
VERSION     KHUT01012690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          104 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggccctt
       61 taagtctgtc ggtcaaatgc cggggctcaa ccccgtgctc gccggagata ctggggggct
      121 ggagacaggt agaggcgagt ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggctcgctgg gcctggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012691             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012691
VERSION     KHUT01012691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           83 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggcggtgaga
       61 taagtcagtc gtgaaatctc ttggcttaac caatgaaatt gcgattgata ctgtttcact
      121 tgaaagaggt tgaggtcagc ggaatgtggc atgtagcggt gaaatgcata gatatgccat
      181 agaacaccga ttgcgaaggc agctggctgg gcctactttg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012692             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012692
VERSION     KHUT01012692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          105 g           42 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtggctctc
       61 gaagtcctgt cgtgaaaggt cgcggcccaa ctgcgacagt gcggaggata ctggggagct
      121 tgagggcagc agaggccggt ggaattcccg gtggagtggt gaaatgcgta gatatcggga
      181 ggaacaccag aggcgaaggc ggccggctgg gctgtacctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01012693             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012693
VERSION     KHUT01012693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           92 g           44 t
ORIGIN      
        1 cacgtagggt gcaagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcgggt gtgaaaaccc caggctcaac ctggggacgc cacccgatac tgctatagct
      121 agagtccagt aggggagtgt ggaattccca gtgtagcggt gaaatgcgca gatattggga
      181 ggaacaccag cggcgaaggc ggcactctgg gctggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012694             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012694
VERSION     KHUT01012694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           54 c           84 g           45 t
ORIGIN      
        1 tacggagaac tcaagcgtta cccggaatca ctgggcgtaa agcgttgcgt aggcggactc
       61 gcaagtcgga tcttaaaggc caaggctcaa ccttggaact gggtacgata ctacgagact
      121 agaggatggt agaggtgagt ggaattgctg gtgtaggagt gaaatccgta gatatcagca
      181 ggaacaccaa tggcgaaggc agctcactgg gccattcctg acgctgaggc acgaaagcgt
      241 agggagcgaa ccgg
//
LOCUS       KHUT01012695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012695
VERSION     KHUT01012695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          104 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgcgg
       61 caagtctgtg gtgaaagctc gtggctcaac tgcgagtcgg ccatggatac tgctgtgctc
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012696             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012696
VERSION     KHUT01012696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           96 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagctcgta ggcggctgag
       61 taagtcaggt gtgaaacccc caggctcaac ctggggccgc catctgatac tgctctagct
      121 tgagtccggt aggggaccac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtggtctgg gccggcactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012697             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012697
VERSION     KHUT01012697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           67 c           99 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggccgg
       61 cccgtcgagt gctgaaatcc cccggctcaa ccggggaagg ggcctcgaaa cgaccggcct
      121 ggagcgaggt agggggagcc ggaactcacg gtggagcggt gaaatgcgtt gagatcgtga
      181 ggaacgcccg tggcgaaagc gggctcctgg acctccgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012698
VERSION     KHUT01012698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggctcga
       61 taagtctggt gtgaaagccc ggggctcacc cccggaagtg cactggatac tgttgagctc
      121 gagttccgga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012699             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012699
VERSION     KHUT01012699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggctatt
       61 gcaagtcggg tgtgaaagcc ctgggctcaa cctaggaagt gcactcgaaa ctgcgaagct
      121 tgaagtctgg agaggaacgc ggaattctcg gtgtagaggt gaaattcgta gatatcgaga
      181 ggaacaccgg tggcgaaggc ggcgttctgg acagcacttg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012700             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012700
VERSION     KHUT01012700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           90 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ttgggcttaa agagcgcgta ggcggcgtcg
       61 taagttggag gtgaaatccc atcggctcaa ccgtggaact gcccccaata ctgcgacgct
      121 tgagggaggt aggggcatgc ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc ggcatgctgg atctcttctg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01012701             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012701
VERSION     KHUT01012701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           82 g           57 t
ORIGIN      
        1 gacgagagct ccgagcgtta tccggaatta ctgggcgtaa agggtgtgta ggtggcattg
       61 ttagtctttt gttaaaatcc caagcctaac ttgggaactg cgaaggatac ggcaatgctt
      121 tgagagtgca agaggcgtgc ggaactcatg gtgtaggggt gaaatccgtt gatatcatgg
      181 ggaacaccaa atgcgaaggc agcacgctgg tgcatttctg acactcaaac acgaaagcgt
      241 gggtagcgaa tggg
//
LOCUS       KHUT01012702             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012702
VERSION     KHUT01012702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           51 c          108 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggtggcttgt
       61 taggttcgtc gttaaagccc gaggcttaac tttgggtggt cggcggagac cggcgggctt
      121 ggaggctggt agagggatgc ggaattcccg gtgtagtggt gaaatgcgta gagatcggga
      181 ggaacaccag aggcgaaggc ggcatcctgg gccgggcctg acactgaggc gcgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01012703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012703
VERSION     KHUT01012703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agagcacgta ggcggcgtcg
       61 taagttggag gtgaaatccc acggctcaac cgtggaactg cctccgaaac tgcgatgctt
      121 gagggaggta ggggcctgtg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccagt ggcgaaagcg gcaggctgga tcttttctga cgctgaggtg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012704
VERSION     KHUT01012704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           91 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggcgcgta ggcggtcata
       61 taagttgggt gtgaaattct cgggcttaac ctggggtggt cactcaaaac tgtctggcta
      121 gagtagggaa gaggaaagtg gaattctcgg tgtagcagtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggt ccactactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012705
VERSION     KHUT01012705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           50 c           74 g           55 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggttccttgg
       61 taagtctgtt gttaaaaccc agagcttaac tctggttcag caatggaaac tgtcaaggta
      121 gaggacggaa gaggcacacg gaattgcagg tgtagtcgta ataagcgttg atatctgcaa
      181 gaacaccaaa agcgaaggca gtgtgctggt acgttcctga cactcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012706             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012706
VERSION     KHUT01012706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           63 c          111 g           31 t
ORIGIN      
        1 gacgtagggg gcgagcgtta tccggattta ttgggcgtaa agggcgcgta ggcggctccc
       61 agcagtggcg cgtggaatcc cccggctcaa ccgggggggg ccgcgccaga cgggggagct
      121 cgaggtgggg agagggtcgc ggaacgggcg gagtaggggt gaaatccgta gagaccgtcc
      181 ggaacaccgt tagcgaaggc ggcgacctgg gcccatcctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cgga
//
LOCUS       KHUT01012707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012707
VERSION     KHUT01012707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           86 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agggaacgca ggcggtcttt
       61 taagtctgat gtgaaagcct tcggcttaac cggagtagtg cattggaaac tggaagactt
      121 gagtgcagaa gaggagagtg gaactccatg tgtagcggtg aaatgcgtag atatatggaa
      181 gaacaccagt ggcgaaagcg gctctctggt ctgtaactga cgctgaggtt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012708
VERSION     KHUT01012708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgag
       61 taagttggat gtgaaatgcc tgggcttaac ctgggatctg catacaaaac tgctcggcta
      121 gagtaaggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccattactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012709
VERSION     KHUT01012709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           85 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcttgg
       61 taagtcagat gtgaaagctc agggctcaac cctgaaattg catccgatac tgccaggcta
      121 gagaactgga gaggtgtcta gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaataccaat ggcgaaggca ggacactgga cagtatctga cgctgaggca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012710
VERSION     KHUT01012710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           88 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcggccgtt
       61 caagtcggat gtgaaagccc ggggctcaac cccggaattg cattcgaaac tggacggctt
      121 gagtacggga gagggaagca gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaatacctgt ggcgaaagcg gcttcctggc ccgatactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012711
VERSION     KHUT01012711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           83 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggattta ttgggcgtaa agggcgcgta gacgggagga
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tgtttttcta
      121 gaggatggac ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagt ttattcctga cgttgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012712
VERSION     KHUT01012712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           88 g           47 t
ORIGIN      
        1 tacgaaccgg acgaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggccttg
       61 taggttgggt gtgaaatccc acggctcaac cgtggaactg cgctcaaaac cacaaggctt
      121 gaggaaggca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaggcg gctcactggg ccttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012713
VERSION     KHUT01012713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggtttgt
       61 taagctagct gtgaaagccc cgggctcaac ctgggaactg cagttagaac tggcaagcta
      121 gagtacagta gaggggtgtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacatcagt ggcgaaggcg acaccctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012714
VERSION     KHUT01012714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           98 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgca ggcggtccgt
       61 taagtgcagg gtgaaagctc ccggctcaac tgggagaggt ccttgtatac tggcggactt
      121 gaggagggta gaggagagtg gaatctccgg tgtagtggtg atatgcgtag atatcggagg
      181 gaacaccagt ggcgaaggcg gctctctggg ccctacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012715
VERSION     KHUT01012715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggatatt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg cctccgatac tgggtatctt
      121 gagtccgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc tcggaactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012716
VERSION     KHUT01012716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           93 g           48 t
ORIGIN      
        1 tacagaggcc ccgagcgtta cgcggtgtca ttgggcttaa agggtgcgta ggcgggtccg
       61 caagtgcttt gtgaaatccc acggctcaac cgtggaatcg cggggcatac tgcgggtctt
      121 gaggccagta agggttgccg gaactgtagg tgtagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg ggcaactggg ctggtactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012717
VERSION     KHUT01012717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          100 g           43 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttcgt
       61 taagtcggga gtgaaagcgc tgggctcaac ccagcaatgg ctctcgagac tggcgggcta
      121 gagggtccta ggggattgtg gaattccagg tgtagcggtg aaatgcgtag atatctggga
      181 gaacacccgt ggcgaaggcg gcaatctggg ggacacctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012718             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012718
VERSION     KHUT01012718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           89 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttctt
       61 taagtctttt gtgaaatctg cgggctcagc ctgtcaaagt gcagaggaaa ctggagaact
      121 agagtgtcag agaggatagc ggaattcccg gtgtagaggt gaaattcgta gagatcggga
      181 ggaacaccag tggcgaaggc ggctatctgg ctgacaactg acgctgagac tcgaaagcat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01012719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012719
VERSION     KHUT01012719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggactat
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catccaatac tgatagacta
      121 gagtgcggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012720
VERSION     KHUT01012720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          100 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ctgggcgtaa agcgcgcgca gacggctttc
       61 tgcgtccggg gtgaaaaccc ggggctcaac cccgggacgg ccttggatac gggagagctt
      121 gagggtcgga gaggcaaggg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacacctgt ggcgaaggcg ccttgctggc cgattcctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012721
VERSION     KHUT01012721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           83 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaatcct ccagcttaac tggagtcgtg ccattgatac tggaaagctt
      121 gagtacaggc gaggttggcg gaattgaagg tgtagcggtg aaatgcatag atatcttcaa
      181 gaacaccgat tgcgaaggca gctaactaga ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01012722             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012722
VERSION     KHUT01012722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           83 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgcta tccggaatca ctgggcgtaa agagtgcgta ggtgggtgtt
       61 taagtgcaat gcgaaattct ggggctcaac cccatgaatg gtattgcata ctggatacct
      121 agaggatatg agaggcaagt ggaattaccg gtgtagcagt gaaatgcgta gagatcggta
      181 ggaacaccaa tggcgaaggc agcttgctgg catatttctg acactaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012723
VERSION     KHUT01012723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccgcg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgcgtggcta
      121 gaggatgaga gaggaaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctttctggc tcatttctga cgctaaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012724             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012724
VERSION     KHUT01012724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           85 g           52 t
ORIGIN      
        1 tacgagtgct tcaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtcttgt gtaaaatctc ccggctcaac cgggaactcg cacgggaaac ggcacgactg
      121 agaggacggc agaggtttct ggaactcatg gtgtagcggt gaaatgcgtt gatatcatgg
      181 ggaacaccga aagcgaaggc aagaaactgg gccgctcctg acactgaaac acgaaagcgt
      241 gggtcgcgaa tggg
//
LOCUS       KHUT01012725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012725
VERSION     KHUT01012725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           87 g           48 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatca ctgggtgtaa agggtgcgta ggcggatgga
       61 taagtcagcg gtgaaatctc acggctcaac cgtgaaactg ccgatgatac tgcccatctt
      121 gagtttggga gaggtcgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcgactggc ccacaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012726
VERSION     KHUT01012726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           91 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtttat
       61 taagtcttct gtttaatgca tgggctcaac ccgtgtacgc aggggaaact ggtagtactt
      121 gagtgcagga gaggaaagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012727
VERSION     KHUT01012727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           89 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttaga
       61 taagtcttgt gtgaaatccc tcggctcaac cgaggaagtg catgggaaac tatctagctg
      121 gaagacggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctgga ccgatcttga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012728
VERSION     KHUT01012728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           94 g           42 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtctct
       61 taagtcggat gtgaaatcct gcggcctaac cgcggaactg catccgatac tgggagactt
      121 gaggacggta gaggtcggtg gaattcccgg tgtaccggtg aaatggacag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01012729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012729
VERSION     KHUT01012729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           94 g           44 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcttgg
       61 taagtcggac gtgaaaacac gaggctcaac ctcgtgcctg catccgaaac tgcccggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012730
VERSION     KHUT01012730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           84 g           60 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtttgta ggcggttatg
       61 taagctagtg gtgaaagcct ggggctcaac ctcagaattg ccattagaac tacatagctt
      121 gagagtgtta gaggaaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gctttctggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012731
VERSION     KHUT01012731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           88 g           44 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctccg
       61 caagtcggat gtgaaatccc tcggctcaac caaggaagtg catccgaaac tgcagggctt
      121 gaatacagaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg ctgatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012732
VERSION     KHUT01012732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           90 g           46 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacggg
       61 taagtacctt gtgaaatccc ccggctcaac cggggaactg ctgggtatac tgcttgtctt
      121 gagctgctta ggggcgaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaaa ggtgaaaaca ggtcgctggg agcatgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012733
VERSION     KHUT01012733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           96 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaactg ccgtggatac tggcggcctc
      121 gagggtgata ggggtcggtg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg accgactgga tcacttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012734             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012734
VERSION     KHUT01012734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           95 a           25 c           66 g           68 t
ORIGIN      
        1 tacgggagga gcgagcatta ttcggaatga ttaggcgtaa agggtttgta ggtggttttt
       61 taagttggaa aaaaaaaatt aaagcccaac tttatacatt ttttcaaaac tgaaaaactt
      121 gagtataaat agaggataat ggaatttcta ttggagtgat aaaatatgtt gataatagaa
      181 ggaagatcga gagcgaaggc aattatctgg gtatatactg acactgagaa acgaaagctt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012735
VERSION     KHUT01012735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           95 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggccgtt
       61 taagattgat gtgaaatccc cgggcttaac ctgggaacgg catcgataac tggacggctg
      121 gagtactgta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctgcctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012736
VERSION     KHUT01012736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           80 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttctt
       61 taagtcagtg gtgaaagcct ccagcttaac tggagaaatg ccattgatac tgaagaactt
      121 gagtacagac gaggtaggcg gaattgacag tgtagcggtg aaatgcatag atattgtcaa
      181 gaacaccgat tgcgaaggca gcttactaga ctgtaactga cgctgaggca cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01012737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012737
VERSION     KHUT01012737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           95 g           44 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtcgca
       61 tcagtctggg gtgacagttc ccggcctaac cgggaaagtg ctctggaaac tgtgcgactt
      121 gagggcttca gagggatgtg gaattccagg tggagtggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gcatcctggg aagttcctga cgctcgggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012738
VERSION     KHUT01012738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           63 c          109 g           33 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtcgcg
       61 caggtcgtac tgtgaaagcg cccggctcaa ccgggcgtgg cgggcgaaac cgcgcgactt
      121 gagggcggta gagggcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacacccgt ggcgaaggcg gccgcctggg ccgaccctga cgctgaggcg cgaaggccgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012739
VERSION     KHUT01012739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c          100 g           37 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggcggacggg
       61 caagcgggat gtgaaagctc ctggctcaac tgggagagtg cgttccgaac tgcgtgacta
      121 gaggccaggc gatgggggtg gaattcccgg tggagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgcaagcg gcccccaagc ctgagcctga cgctgaagcg cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012740
VERSION     KHUT01012740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggtggcacca
       61 taagtctgct gtgaaagcgc ccggctcaac tgggcgaggt cggtggaaac tgtggagcta
      121 gaggacggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg ccgctcctga cactgagacg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012741
VERSION     KHUT01012741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggcttg
       61 taagtcgatt gtgaaagccc cgggctcaat ccgggaattg cagtcgaaac tataagtctt
      121 gaatgctcta gagggtgacg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gttgcctgga gatgcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012742
VERSION     KHUT01012742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           63 c           86 g           41 t
ORIGIN      
        1 taccagcacc ccgagtggtc gggacgatta ttgggcctaa agcatccgta gccggttccg
       61 caagtcttcc gttaaatcca cctgctcaac agatgggccg cggaggatac cgcggagcta
      121 ggaggcggga gaggcaaacg gtactcagtg ggtaggggta aaatccattg atccactgaa
      181 gaccaccagt ggcgaaggcg gtttgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012743
VERSION     KHUT01012743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          103 g           42 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggcggatgtc
       61 taagtctgtt gtgaaagcgc ctggcttaac cgggcgaggt cgatggaaac taggcgtcta
      121 gaggccggta gaggggggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccgcgcctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012744
VERSION     KHUT01012744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           99 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcttgc
       61 caagtcagtg gtgaaatctc ccggcttaac tgggagaggc ccgctgatac tggtaggctc
      121 gagggaggca gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg accttctggg ccttgcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012745
VERSION     KHUT01012745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          103 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcgtcg
       61 gcagtctgtg gtgaaagctc gtggctcaac tgcgagtcgg ccatggatac tccgatgctt
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtagctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012746
VERSION     KHUT01012746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          100 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtctgc
       61 taagtctggg gtgaaagctc ccggctcaac tgggagaggt ccctggatac tggcagactc
      121 gagggaggta gaggagagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gctctctggg ccttacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012747
VERSION     KHUT01012747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgaag
       61 caagtcggat gtgaaagctc cgggctcaac ccggaaatgg cattggaaac tgctttgctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012748
VERSION     KHUT01012748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           93 g           47 t
ORIGIN      
        1 tacagagggg gccagcgttg ttcggaatca ctgggtgtaa agggtgcgca ggtggtcagt
       61 tcagtctgat gtgaaatccc tgggctcaac tcaggaatgg cattggaaac tggctggcta
      121 gagtaccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gcttcctggc cggatactga cactcatgca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012749
VERSION     KHUT01012749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           92 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtcgtt
       61 tgtgtcgggt gtgaaatccc acggctcaac cgtggaatgg cattcgaaac tggacggcta
      121 gagtgcagga tgggagagcg gaattcttgg tgtagcggtg aaatgcgttg atatcaagaa
      181 gaacaccggt ggcgaaggcg gctctctgga atgcaactga cactgaggca cgaaagctgg
      241 ggaagcaaac agg
//
LOCUS       KHUT01012750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012750
VERSION     KHUT01012750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           80 g           56 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgagca ggcggatata
       61 taagttagat gtgaaatccc tgggcttaac ctaggaacag catttaagac tgtatgtcta
      121 gagtttgtga gaggtgggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca gcccactggc atacaactga cgctcaatca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012751
VERSION     KHUT01012751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           55 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggcggcctgt
       61 taagtaagtt gtgaaatccc acggctcaac cgtggaattg caatttaaac tggcgggctt
      121 gaatgcagga gaggttggta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccggt ggcgaaggcg gccaactggc ctagcattga cgctgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012752
VERSION     KHUT01012752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           98 g           44 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggttgct
       61 taagtccgct gtgaaatcac caggctcaac ctggtgcagt cggtggaaac taggcgactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012753
VERSION     KHUT01012753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggttgag
       61 taagtcagat gtgaaatccc tgagcctaac tcaggacgtg catttgaaac tgctcgactc
      121 gagtgcagta gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccgat ggcgaaggca gccaactggg ctgcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012754
VERSION     KHUT01012754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggattta ctgggcgtaa agcgtgcgta ggcggcattg
       61 agagtcgcgg gtgaaatccc agggcttaac cctggaactg cctgcgagac ctcagtgcta
      121 gagtgagtga ggggttggtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacacctgt ggcgaaggcg gccaactggc actcaactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012755
VERSION     KHUT01012755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          106 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtcttt
       61 taagcgtgtg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgggagactg
      121 gagcatgtca gaggcgggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg acatggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012756
VERSION     KHUT01012756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           95 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 ccagtcaggg gtgaaatcct ccagctcaac tggagcagtg cctttgatac tgcgagtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012757
VERSION     KHUT01012757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           36 c           74 g           68 t
ORIGIN      
        1 tacaggggat gcgagtgtta ttcggaatta ttgggcgtaa agcgtccgta ggtggatctt
       61 ttagtcattt gttaaatttt tgagcttaac tcaaagcctg caaatgaaac tattgatctt
      121 gagtatggta aaggtaaagg gaacttccag tggagcggtg gaatgtgtag atgttggaaa
      181 gaacaccgat ggcgaaggca ttttacttgg ccagtactga cactgataga cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012758
VERSION     KHUT01012758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           79 g           59 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtgatg
       61 taagtctgat attaaataca gaagcttaac ttctgacctg tatcggaaac tacattacta
      121 gaggacaata gaggaagtgg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg cacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012759
VERSION     KHUT01012759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           75 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggattta
       61 taagtcagtg gtgaaagcct acagctcaac tgtagaactg ccattgatac tgtaagtctt
      121 gaatttggtc gaagtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat agcgaaggca gctcactagt ccaacattga cgctcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012760
VERSION     KHUT01012760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttaga
       61 taagttggat gtgaaatccc cgggcttaac ctgggcaggt catccagtac tgtcagacta
      121 gagtacagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttcctgga ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012761
VERSION     KHUT01012761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           88 g           50 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agggtgtgca ggcggtctat
       61 caagtcagat gtgaaataac tcggctaaac cgggtggggt catttgaaac tggtagacta
      121 gagtagtgca gaggatagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctggg cacaaactga cgctgagaca cgaaggctaa
      241 gggagcaaac agg
//
LOCUS       KHUT01012762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012762
VERSION     KHUT01012762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           98 g           44 t
ORIGIN      
        1 tacgtaggat gctagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcacac
       61 taagtatggt gtgaaatctc cctgctcaac ggggagggag cattgtaaac tggtgagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012763
VERSION     KHUT01012763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           85 g           57 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggcgggtttt
       61 taagtcagtt gtgaaagtct gcggctcaac cgtagaattg cagttgaaac tggaagcctt
      121 gagtgcacgc aggggtgccg gaattcatgg tgtagcggtg aaatgcttag atatcatgaa
      181 gaactccgaa agcgaaggca agtgcccgga gtgcaactga cgctgaggct cgaaagtgcg
      241 ggtatcaaac agg
//
LOCUS       KHUT01012764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012764
VERSION     KHUT01012764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgca ggcggacagg
       61 taagtcagtg gtgaaatcct gcagcttaac tgcagaactg ccgttgatac tatttgtctt
      121 gaatgttgtg gaggttagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctaactacg caaatattga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012765
VERSION     KHUT01012765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggtttgc
       61 gaagtctcta ccgacagtgt ccggcttaac cgggcaagtg gtagggaaac tggcaaactt
      121 gagggcttca gagggatgtg gaactccagg tggagtggtg aaatgcgtag atatctggaa
      181 gaacaccaaa ggcgaaggca acatcctggg aagcatctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012766
VERSION     KHUT01012766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           85 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcgggcctt
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tggaggtctt
      121 gagtgccgga gaggaagatg gaacgggtcg tgtagcggtg aaatgcgttg agatgactcg
      181 gaacaccaat ggcgaaagca atcttctgga cggaaactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01012767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012767
VERSION     KHUT01012767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          104 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggtgggg
       61 tcagttcgtg gtgaaagcgc ggggctcaac cccgcgtcgg ccatgaatac tgccgcactt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012768
VERSION     KHUT01012768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          105 g           34 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggctgc
       61 cgcgtccggg gtgaaagcct cccgctcaac gggagaacgg cctcggatac gggcggcctc
      121 gaggggggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccccgtctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012769
VERSION     KHUT01012769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          103 g           40 t
ORIGIN      
        1 gacagagggg gccagcgttg tccggattga ctgggcgtac agcgcgcgca ggtggatact
       61 gacgtgccgt gtgacagcca ggagctcaac ttctggtggc cacggtaaac gcagtgtctg
      121 gagggtgcga gaggcatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcatgctggc gcattcctga cactgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012770
VERSION     KHUT01012770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          103 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggacgtt
       61 taagtgtggc ctgaaagccc ccggctcaac cggggagggc ggctgcaaac tgggcgtctt
      121 gaggaaccaa gaggcgtgtg gaattccggg agtagcggtg aaatgcgtag agatccggag
      181 gaacacccgt ggcgaaggcg gcacgctggc ggttacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012771
VERSION     KHUT01012771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcatgta ggcgggaggg
       61 tgtgtcgaac gtgaaaggct gcggctcaac cgcagagcgc cgttcgaaac tgcccttctt
      121 gagggccgaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacccat ggcgaaggca gctctctggt cggcctctga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012772
VERSION     KHUT01012772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctt
       61 caagtccggg gtgaaagcct cccgcttaac gggagaagag cctcggagac tgagggcctc
      121 gaggagggta ggggcgtgcg gaactatggg tggagcggtg aaatgcattg atatccatag
      181 gaactccggt ggcgaaggcg gcacgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012773
VERSION     KHUT01012773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           63 c          103 g           32 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcgggtgcc
       61 gacgtgccgt gtgacaaacg ggggctcaac ccccgagggc catggtacac gagacacctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01012774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012774
VERSION     KHUT01012774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          100 g           39 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtcg
       61 ccagtctgtg gtgaaatccc ccggctcaac cggggaatgg ccgtggatac tgcgactctc
      121 gaggggtgta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga cacctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012775
VERSION     KHUT01012775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           82 g           51 t
ORIGIN      
        1 gacgaagggg gctagcgtta ttcggaatta ctgggcgtaa agagcgcgta ggtggtttaa
       61 caagttggaa gtgaaagccc aaggctcaac cttggaactg ctttcaagac tgttagacta
      121 gagtatggta gagggtgatg gaattcctag tgtagaggtg aaattctgag acattaggag
      181 gaacaccaga ggcgaaggcg atcacctggg ccactactga cactaatgcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012776
VERSION     KHUT01012776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccagg
       61 caggtctgct gttaaaactc gaggctcaac ctcgagaggt cggtggaaac cgtttggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012777
VERSION     KHUT01012777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           79 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggagggcagg
       61 taagtcagcg gtgaaatctc tgggcttaac ccggaaactg ccattgatac tatctgtctt
      121 gaatatggtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgctaca cctatattga ctctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012778
VERSION     KHUT01012778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           86 g           48 t
ORIGIN      
        1 gacgaaccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgca ggcggcctga
       61 caagttgggt gtgaaatacc tcggcttaac cggggaatgg cgctcaaaac tgtcgggctt
      121 gagggagata gaggtgagcg gaacttaagg tggagcggtg aaatgcgttg atatcttaag
      181 gaacacctgt ggcgaaagcg gctcactgga tctcttctga cgctcaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012779
VERSION     KHUT01012779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggtgcgta ggcgggtttt
       61 caagtcgggg gtgaaatctt tcggcccaac cggaaaaggg cctccgatac tgggaacctc
      121 gagggagata ggggcgcgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg gcgcgctgga tctcttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012780
VERSION     KHUT01012780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           83 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgagcgca ggcggacctt
       61 taagtaggaa gtgaaaggtt ggagctcaac tctaacactg ctccctatac tggaggtctt
      121 gagtgccgga gaggaagacg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca gtcttctgga cggatactga cgctcaggct cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01012781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012781
VERSION     KHUT01012781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           84 g           56 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggcgtttcgt
       61 taagtctcca gtgaaagccc tcggctcaac tgaggaatgg ttggagatac tggcggaatt
      121 gagtatggca gaggcagatg gaattctcgg tgtagcggta aaatgcgttg atatcgagaa
      181 gaacaccagt ggcgaaggcg atctgctggg ccattactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012782
VERSION     KHUT01012782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           39 c           83 g           64 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggtttgt
       61 taagtcagtc gttaaaggca gcagcttaac tgttgtaagg cgattgatac tggcgaactt
      121 gaattattgg gaggttgtcg gaatgcgtag tgtagcggtg aaatgctcag atattacgca
      181 gaacgccgat tgcgaaggca gataactacc aatatattga cgctgaggca cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012783
VERSION     KHUT01012783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           51 t
ORIGIN      
        1 gacataggtg gcgagcgtta ttcggaatta ctaggcgtaa agcgagtgta ggcgggcgct
       61 taagtccgac gtgaaatctc ccggcttaac tgggaggggt cgtcggatac tgggcgtctt
      121 gagtggggta gggggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca gctgcctggg cctttactga cgctaagact cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012784
VERSION     KHUT01012784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           85 g           54 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ctcggaatca ttgggcgtaa agcgggtgca ggtggctttg
       61 taagtcagga gtgaaagccc agggcttaac cctggaagtg cttttgatac tgcaaagctt
      121 gagtgtgaga gaggatgctg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaaccccggt ggcgaaggcg ggtatctggc tcaacactga cacttagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012785
VERSION     KHUT01012785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          112 g           31 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggagtta ctgggcgtac agcgcgcgca ggcggccgtg
       61 aaggtgccgg gtgacagacg ggggcttaac ccccggcggt cccggtagac cggacggctg
      121 gaggctggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcatcctggc ccagtactga cgctgaggcg cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012786
VERSION     KHUT01012786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           99 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agggcaagta ggtggttcag
       61 tgcgtggggt ttgaaaacac ctggcttaac tgggtgggga ggccccaaac ggctggactt
      121 gaggtaggga gagggatgcg gaattcctgg tgtagtggtg gaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaagcg gcatcctggc cctgacctga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012787
VERSION     KHUT01012787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ttgggcgtaa agcgcgtgta ggcggttcga
       61 ttagtccgct ctgaaagccc agggctcaac cctgggaggc gggtggatac tgtcggacta
      121 gagtacggaa ggggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac agcgaaggca gatctctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012788
VERSION     KHUT01012788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           82 g           58 t
ORIGIN      
        1 tacgagagcc tcgagcgtta tccggaatta ttgggcgtaa agggtgcgta ggttgttctg
       61 ttagtctttt gtcaaagccc cgggcttaac ctgggatatg cgagagaaac ggcagaactt
      121 gaaagtgcga gaggtatacg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca gtatactggc gcatatttga cactgaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01012789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012789
VERSION     KHUT01012789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           82 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgca ggcggaagag
       61 taagtaggaa gtgaaaggtc ggagctcaac tccaacactg ctccctatac tgcttttctt
      121 gagtgccgga gaggaagagg gaacttcacg tgtagcggtg aaatgcgttg agatgtgaag
      181 gaacaccaat ggcgaaagca ctcttctgga cggaaactga cgctgagata cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01012790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012790
VERSION     KHUT01012790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           83 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggattga
       61 taagtcagat gtgaaatacc aaagctcaac tttggtgctg catttgaaac tgtctctcta
      121 gaggattgac ggagaaagcg gaattccacg tgtagcggtg aaatgcgtag atacgtggag
      181 gaacaccggt ggcgaaagcg gctttctagt ttagacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01012791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012791
VERSION     KHUT01012791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           38 c           73 g           60 t
ORIGIN      
        1 gacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta ggtggtttgt
       61 taagttggat gttaaagctt tgagctcaac tcaaaattgg tatccaaaac tggcaggcta
      121 gagacaggta gaggtagata gaattctgta tgtaggggta atatccgtag atatacagag
      181 gaataccaaa agcgaaggca atctactgga cctgttctga cactaaagaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012792
VERSION     KHUT01012792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggagtgc cgcccgagac tgcctggctt
      121 gaggtcggaa gaggggagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccgattctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012793             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012793
VERSION     KHUT01012793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c          100 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatcgg
       61 cgcgtcggtc gctgaaagcc cccggctcaa ccggggaata ggcgtcgaaa cggccggtct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg cggcgaaagc gaggtcctgg acgccatctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012794
VERSION     KHUT01012794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggccgcg
       61 taagtcgggc gtgaaatccc acagctcaac tgtggaagtg cactcgaaac tgtgcggcta
      121 gagtcctgga gaggaaggtg gaatacttgg tgtagaggtg aaattcgtag atatcaagtg
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012795
VERSION     KHUT01012795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcggctttc
       61 taagtcgggg gtgaaatccg gcagctcaac tgtcgcagtg ccttcgatac tgggaagctc
      121 gagggaggta ggggtgcgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctcaactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012796             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012796
VERSION     KHUT01012796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           83 g           53 t
ORIGIN      
        1 tacgtagacc tcgagcgtta tccggattta ctgggcgtaa agcgtgcgta ggtgtctttg
       61 taagtctctg gtgaaatctc cgggctcaac ccggaaatgg tcagggaaac tgcaaggatc
      121 gagtacggca gaggcagatg gaatggttgg tgtagcggta aaatgcgttg atatcaacca
      181 gaacaccaat ggcgaaggca atctgctggg cctgtactga cactgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012797
VERSION     KHUT01012797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtag agggcgcgta ggctgtttga
       61 taagtagaag gtgaaagatt cgggctcaac ccgaaaactg ccttctaaac tgtcttactt
      121 gagggtctga ggggaagctg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaagcg ggcttctggc agactcctga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012798
VERSION     KHUT01012798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           62 c          103 g           37 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggcgccg
       61 tatgtgactg gtgacattcg cgggctcaac ccgcgacggc ccggttacac agcggtgctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgatagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012799
VERSION     KHUT01012799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           81 g           52 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatta ctgggcgtaa agcgcatgta ggtggttatg
       61 caagtcgacg atgaaagact accgcttaac ggtaggaccg ttttcgatac tacatagctt
      121 gaggaatgca ggggtaagcg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca gcttactggg cattacctga cactaagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012800
VERSION     KHUT01012800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c          101 g           36 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcgggtagg
       61 caagtcggaa gtgaaagccc ggggctcaac cccgggaggc ctttcgatac tgcctgccta
      121 gggaccggta gaggctggtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacacccgt ggcgaaggcg gccagctggg ccggatccga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012801
VERSION     KHUT01012801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           93 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatca ctgggcttaa agcgtgcgta ggcggacatg
       61 caggcatgga gtgaaatccc atggctcaac catggaacag cttcgtgaac cgcatgtctt
      121 gaggcaggta ggggcggtcg gaacgatagg tggagcggtg aaatgcgtag atatctatcg
      181 gaacgccgac ggagaagtca ggccgctggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012802
VERSION     KHUT01012802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           84 g           57 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgcgta gacggcatat
       61 taagtcaact gttaaattcc ctggcctaac tggggaccag cagtagatac tgatgtgctt
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012803
VERSION     KHUT01012803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           91 g           48 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggctcgt
       61 taagtgtgga gtgaaatctc ggggctcacc cccgaaactg ctctacatac tggcgagctg
      121 gagggacgaa gaggcttgtg gaattcccgg tgtagcggtg aaatgcgttg atatcgggag
      181 gaacacccat ggcgaaggca gcaagctggt cgtctcctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012804
VERSION     KHUT01012804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggttcga
       61 ttagtcagat gtgaaagccc tgggcttaac ccgggaactg cgtttgatac tgtcgagcta
      121 gaattcggaa gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggt ccgacattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012805
VERSION     KHUT01012805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           46 t
ORIGIN      
        1 gacgaaggtg gctagcgtta ttcggattta ctgggcataa agcgcacgta ggcggccttg
       61 taagtcggag gtgaaagccc gcagctcaac tgcggaattg cccccgatac tgcttggctt
      121 gagtgcagga ggggtgactg gaactccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaga ggcgaaggcg ggtcactgga ctgcaactga cgctgaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01012806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012806
VERSION     KHUT01012806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggagcg
       61 cgcgtccgag aggaaatctc acggctcaac cgtgtggctt tctcggatac ggcgcttctt
      121 gagggtagaa gaggggactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtccctggt ctacatctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012807
VERSION     KHUT01012807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           98 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggccaag
       61 tatgtcgtgc gtaaaatctc ccggctcaac tgggaggggc cgcgcgaaac tgcttggctt
      121 gagttcggta gggggaagtg gaattccggg tgtagtggtg gaatacgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01012808             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012808
VERSION     KHUT01012808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           46 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggttccta ggcggctggc
       61 ctagtcgggt gtgaaacctc caggctcaac ctggagacgc cacccgatac tggtctggct
      121 agagtccggt aggggcgaac ggaattcccg gtgtagcggt gaaatgctta gatatcggga
      181 ggaacaccag tggcgaaggc ggttcgctgg gccggaactg acgctgaaga acgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01012809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012809
VERSION     KHUT01012809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           93 g           52 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agggttcgta ggcgggagag
       61 taagttaagt gtgaaagacc tgggctcaac ctgggaggtg cacttaaaac tgtttttctt
      121 gagtctcaga gagggtggtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacatcgga ggcgaaggcg accacctggc tgaagactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012810
VERSION     KHUT01012810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           50 c           99 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctcgt
       61 ttagtcggat gtgaaagtcc agggctcaac cctgggatgc cattcgatac tggcgagctt
      121 gagcacggta gggggatctg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctgga ccgttgctga cgctgaggcg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01012811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012811
VERSION     KHUT01012811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           96 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctgct
       61 caagtcgaat gtgaaaactc gaggctcaac ctcgagcctg cattcgatac tgggcggcta
      121 gagtctggta gggggatctg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggatcctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012812
VERSION     KHUT01012812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           97 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggcggttctt
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccggatac tgaagagcta
      121 gagtgcggga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgcaaacg gctctctgga ccgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012813             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012813
VERSION     KHUT01012813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           55 c           87 g           46 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcgggtgtt
       61 tgcgtgagat gcgaaatccg gtggctcaac catctggact gtatctcata cggggcacct
      121 agaggacggc agaggcaagc ggaattactg gtgtagcagt gaaatgcgta gatatcagta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccacacctg acgctaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012814             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012814
VERSION     KHUT01012814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c          100 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgtgg
       61 taagtcttac tgtgaaagac cggagctcaa ctccggggct gcagaggata ctgccatgct
      121 ggagacaagt agaggcagat ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccag aggcgaaggc ggtctgctgg gcttggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01012815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012815
VERSION     KHUT01012815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c          101 g           39 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggccccg
       61 ccagtctgac gtgaaagccc agggctcagc tctggaaggg cgttagaaac tgcggggctc
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012816
VERSION     KHUT01012816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggacatt
       61 taagatagat gtgaaacacc tgggcttaac ctaggtcgtg catataagac tggatgacta
      121 gagtaatgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actacctgga catatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012817
VERSION     KHUT01012817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           98 g           37 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agggcgggca ggcggcttgt
       61 caagtgggat gtcaaagcgc acagcttaac tgagcgaggc cattccaaac tgatgagctg
      121 gagggcagta gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012818
VERSION     KHUT01012818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacgtaggga gcaagcgttg cccggaatta ctgggcgtaa agagctcgta ggcgggggcg
       61 tgcgtccgag aagaaatctt acggctcaac cgtagggcta tctcggatac ggcgcttctt
      121 gagggtgaga gaggaaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagc ggcgaaggcg gctttctggc tcattcctga cgctgaggag cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012819
VERSION     KHUT01012819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 gacagaggtg gcgaccgttg ttcggaatca ctgggcataa agcgcgtgta ggtggcattg
       61 taagtcgggt gtgaaatccc tcggcctaac cgaggaattg cgcccgaaac tactttgctt
      121 gaggatggga ggggagcctg gaattcctgg tggagcggtg aaatgcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg aggctctggc ccattactga cactgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012820
VERSION     KHUT01012820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           59 c          105 g           41 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggttcac
       61 tcagtccggc gtgaaagctc ccggctcacc tgggagaggg cgtgggatac tggtggactg
      121 gaggcaggca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccggctggg cctgtcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012821
VERSION     KHUT01012821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           57 c          106 g           42 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggttcgg
       61 tacgtccggc gtgaaagctc ccggctcaac tgggagaggg cgttggatac ggccggactg
      121 gagggcggta gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccgactggg ccgtccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012822             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012822
VERSION     KHUT01012822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           55 c           80 g           48 t
ORIGIN      
        1 tacgaaggcc ccgagcgtta tccggattta ttgggcgtaa agcgttcaac ggtggttctg
       61 cacgtccccg gttaaatctc atggctcaac catgaggccg ccggtgatac gacaggactt
      121 gaggacggga gaggcatacg gaatgaccgg tgtagcggta aaatgcgtta atatcggtca
      181 gaacaccaat agcgaaggca gtatgctgga acgatcctga cactaaaaga acgaaagcgt
      241 ggggagcgaa tggg
//
LOCUS       KHUT01012823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012823
VERSION     KHUT01012823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           92 g           43 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcggacggg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgcccgtctt
      121 gaggcactca ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtgtcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012824
VERSION     KHUT01012824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c          104 g           40 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggaatca ctgggcgtaa agggcgtcta ggcggccgct
       61 ttagtcgtgc gtgaaagctc ccggctcaac tgggagaggg cgcacgatac tgggcggctc
      121 gagactcaga ggggggtgtg gaattcccgg tggagtggtg aaatgcgttg agatcgggag
      181 gaacaccagt ggcgaaggcg gcaccctggc tgaggtctga cgctgaagcg cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012825
VERSION     KHUT01012825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggccatc
       61 taagtctgct gtgaaagtcc agggctcaac cctgggatgc cggtggatac tggatggctc
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gcactctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012826
VERSION     KHUT01012826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           90 g           48 t
ORIGIN      
        1 cacgaaccgg gcaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgctg
       61 taagtcgggt gtgaaatccc tcggcctaac cgaggaattg cgcccgatac tgcagtgctc
      121 gaggaagata gaggtgggcg gaactagcgg tggagcggtg aaatgcgttg atatcgctgg
      181 gaacacccgt ggcgaaagcg gcccactggg tcttttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012827
VERSION     KHUT01012827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           71 c          106 g           28 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagcccgcg gtgaaatccc ccggctcaac cgggggacgg ccgcggggac tgacggcctc
      121 gaggggggta ggggcgcgcg gaacgccggg tggagcggtg aaatgcgttg atatccggcg
      181 gaactccggt ggcgaaagcg gcgcgctggg cccccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012828
VERSION     KHUT01012828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttagt
       61 aaagtgtgat gtgaaagccc cgggctcaac ctgggaagtg catcgcaaac agactaactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012829
VERSION     KHUT01012829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agcgcgcgta ggcggcctgc
       61 taagtctaat gtgaaatccc tggacctaat tcaggaagtg cattggatac tggtaggcta
      121 gagtacagga gaggagagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctctctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012830
VERSION     KHUT01012830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           52 c           75 g           52 t
ORIGIN      
        1 tacgaggacc ccgagcgtta tccggaatta ctgggcgtaa aacgtgagca tccggttgtg
       61 ctagtcttat gttaaatctc attgctcaac gatgagaccg catgggaaac ggcacgacta
      121 gagtgggtaa gaggcaagcg gaacgtgcgg tgtaggggtg aaatccattg atattgcacg
      181 gaacaccaaa agcgaaagca gcttgctagt gctctactga cggtcaatca ctaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01012831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012831
VERSION     KHUT01012831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          101 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtc
       61 cgagtccggg gtgaaagccc ccagcctaac tggggaagtg ccttggatac cggatgtctc
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcctgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012832             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012832
VERSION     KHUT01012832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ctcggaatta ctgggcataa agggcgcgta ggtggcttgg
       61 taagtcggtg gtgaaatcct gtggcttaac cacagaactg ctatcgatac tgccgagctc
      121 gaggacggga ggggagaatg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg attctctgga ctgttcctga cactgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012833
VERSION     KHUT01012833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggccttg
       61 caagtcgggt gtgaaagccc tcagctcaac tgaggaagtg cgcccgaaac tgcttggctt
      121 gagtaccgga gagggtcgcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gcgacctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012834
VERSION     KHUT01012834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           94 g           43 t
ORIGIN      
        1 tacgtaggga gctagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggcgcga
       61 taagtccgtt gtgaaagtcg agggctcaac cctcgaatgc cggcggatac tgtcgcgcta
      121 gagtccggaa gaggcgagtg gaactcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccgat ggcgaaggca gctcgctagg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012835
VERSION     KHUT01012835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           89 g           51 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaactg cgtccaatac tgccgtgctg
      121 gagggagata ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactgga tctcttctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012836
VERSION     KHUT01012836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           89 g           45 t
ORIGIN      
        1 tacccgcagc acaagtggtg gtcattatta ttgggcctaa agcgtccgta gctggcgtgt
       61 tagatgcctg ggtaaatcga agcgctcaac gcttcgactt ccgggtagac cggcacgctc
      121 gagaccggga gaggcatggg gtactcgggg ggtaggggta aaatcctgta atcctccggg
      181 gacgaccagt ggcgaaggcg ccatgctaga acggctctga cagtgaggga cgaagccctg
      241 gggcgcaaac cgg
//
LOCUS       KHUT01012837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012837
VERSION     KHUT01012837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggtcttt
       61 taggccgacc gtgaaatccc atggctcaac catggaagtg cggtcggaac cgggagacta
      121 gagtctcgac gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccatctggg agaagactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012838
VERSION     KHUT01012838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcagt
       61 caagtcaagg gtgaaatcct gcggctcaac cgtagaagag cccctgatac tggctgtctc
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012839
VERSION     KHUT01012839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           85 g           51 t
ORIGIN      
        1 tacgaaggtg acaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcgggtttg
       61 taagtcgatt gtgaaatccc tgggctcaac ccgggaattg cagtcgaaac tgcaagtctt
      121 gaatgctgga gaggatggtg gaattccagg agtagaagtg aaattcgtag atatctggag
      181 gaacaccgga ggcgaaagcg gccatctggc cagacattga cgctaaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012840
VERSION     KHUT01012840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agcgtacgta ggcggccttt
       61 taagtcggtg gttaaagact gcggctcaac cgcaggagtg ccttcgatac tgagaggctg
      121 gagggtggca gaggaagtcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg gacttctggg ccattcctga cgctgaggta cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012841
VERSION     KHUT01012841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           81 g           55 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggtctt
       61 taagtcagat gtgaaatccc gaggctcaac ctcggaacag catttgaaac tgaagatctt
      121 gagggtagaa ggagagaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg attctctatt ttattcctga cgctgaggcg cgaaagtaag
      241 gggatcaaac agg
//
LOCUS       KHUT01012842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012842
VERSION     KHUT01012842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c           97 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 atagtttgcg gtgaaatcct ccagcttaac tggagaagtg ccgtgaatac tgccggtctt
      121 gaggagggta ggggcatctg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012843
VERSION     KHUT01012843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ttgggcgtaa agcgcgcgta ggcgggttcg
       61 taagtcgatt gtgaaaaccc tgggctcaac ctgggacgtg cagtcgatac tgcgaatctt
      121 gagtgcctga gaggatggcg gaattcccgg tgtagtagtg aaatacgtag atatcgggaa
      181 gaacacctga ggcgaaggcg gctatctggc ggtgcactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012844
VERSION     KHUT01012844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           80 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggatagt
       61 taagtaagaa gtgaaaggtc ggagcttaac tccaacactg cttcttatac tggctatctt
      121 gagtagcgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacacccat ggcgaaagca ctcttctgga cgcagactga cgctcatgtt cgaaagccaa
      241 gggagcgaac agg
//
LOCUS       KHUT01012845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012845
VERSION     KHUT01012845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacagaggtt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcgggacgg
       61 catgtcgaat gtgaaagccc tcggctcaac cgaggaagtg cgttcgaaac taccgttctt
      121 gagtgtcgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cgaacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012846
VERSION     KHUT01012846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttagt
       61 taagatagat gtgaaagccc tgggcttaac ctaggaacgg catctgttac tgactagctg
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012847
VERSION     KHUT01012847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           90 g           54 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggaatta ctgggcgtaa agggcgtgta ggtggttgaa
       61 taagttggtt gtgaaatccc tgggcttaac ctaggaatgg caatcaagac tgtttaacta
      121 gagtacggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012848             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012848
VERSION     KHUT01012848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           87 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttgga
       61 taagttcttt gtgaaagctc ccggcttaac tgggagaggt caaggaatac tgtctggctt
      121 gagtacggta ggggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca agttactggg ccgttactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012849
VERSION     KHUT01012849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgt
       61 caagtcggtg gtgaaatctt tcggctcaac cggaaaactg ccttcgatac tggcaggcta
      121 gagggagata ggggttgcgg gaactaccag tggagcggtg aaatgcgttg atattggtag
      181 gaacgccggt ggcgaaagcg cgcaactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012850
VERSION     KHUT01012850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           89 g           45 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgcgta ggtggttcgg
       61 taagtcaggt gtgaaatccc ggggctcaac cccggaaggg cacttgatac tgccagactt
      121 gagtactgga gaggagagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actctctgga cagatactga cactgaggcg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01012851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU12999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012851
VERSION     KHUT01012851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU12999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           81 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatca ctgggtttaa agggtgcgta ggtgggtgtt
       61 taagtgagtg gtgaaagctc ccggcttaac cgggaaattg ccattcatac tggatacctt
      121 gagtacagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012852
VERSION     KHUT01012852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           95 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggctctt
       61 gaagtcaggc gtgaaagccc cgggctcaac ctgggaatag cgcttgatac tcgagagctt
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012853
VERSION     KHUT01012853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           91 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggactgg
       61 taagtcgaat gtgaaagccc tgggctcaac ctgggaatgg cactcgatac tgtcagtctt
      121 gaatgcttga gagggtggcg gaattccagg tgtagaagtg aaattcgtag atatctggag
      181 gaacaccgga ggcgaaggcg gctgcctggc aagacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012854             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012854
VERSION     KHUT01012854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           50 t
ORIGIN      
        1 cacgagtggt ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggttgtg
       61 ttagtctcgt gtcaaatcct tcctgcctaa caggaagacc gcacgggata cggcacagct
      121 tgagggtgcg aggggtgaat ggaactcacg gtgtaggggt gaaatccgtt gatatcgtgg
      181 ggaacgccaa aagcgaaggc aattcactgg cgcatacctg acgctgaaac acgaaagcca
      241 ggggagcgaa tggg
//
LOCUS       KHUT01012855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012855
VERSION     KHUT01012855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           82 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggggcgta ggcgggaatg
       61 aaagcaagac gtgaaatccc agggctcaac cctggaactg cattttgaac tacatttcta
      121 gaggataggc ggagaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttattcctga cgctgaggcc cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012856
VERSION     KHUT01012856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           63 c          101 g           34 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggccgtg
       61 cacgtggggt gtgaaagccg gtggcttaac taccggaggc catcccatac ggcgcggctg
      121 gagccatgca gaggccagcg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gctggctggg catgggctga cgctcaggtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012857
VERSION     KHUT01012857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtctgat gtgaaatcct gaggctcaac cttggaactg cattggatac tgctttgcta
      121 gagaactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012858
VERSION     KHUT01012858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           88 g           47 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtgatg
       61 caagtcgggt gtgaaatacc ggagcctaac tccggaacgg cattcgaaac tgcatcgctc
      121 gaggtctgga gaggtgattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaggcg aatcactaga cagaacctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012859
VERSION     KHUT01012859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           97 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttcc
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggggggctc
      121 gagggaggta ggggcaagcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gcttgctgga cctcatctga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012860
VERSION     KHUT01012860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agcgcatgta ggttgtttag
       61 taagtgggcg attaaagacc gatgcttaac atcgggaatg ttacctatac tgcttgacta
      121 gagaatgaca ggggtaagcg gaactttagg tgtaggagtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttactggg tcttttctga cactgagatg cgaaggcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012861
VERSION     KHUT01012861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggtttgg
       61 caagtccgct gtgaaatctc caggctcaac ttggagaggt cggtggaaac tgccaaactt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012862
VERSION     KHUT01012862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c          106 g           33 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggcctct
       61 caagtcgggc gtgaaagccc tcggctcagc cgaggaaggg cgcacgaaac ggaggggctg
      121 gagggccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcccctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012863
VERSION     KHUT01012863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          104 g           38 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggcccgg
       61 caagtcaggc gtgaaatccc cgggctcaac ccgggggctg cgcttgagac tgcggggctg
      121 gagggcggga gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggc ccgtttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012864
VERSION     KHUT01012864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          107 g           34 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggagtaa ctgggcgtac agcgcgcgca ggcggttggc
       61 gacgtgccgt gtcacagccc tgagctcacc tcagggtggc cacggtagac gcgtcgacta
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgaaggcg gcatcctggc cctggcctga cgctgaggtg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012865
VERSION     KHUT01012865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tgcgggtctt
      121 gggccaccta ggggcagacg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gtctgctggg ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012866
VERSION     KHUT01012866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctgt
       61 taagtgtgaa gtgaaatctc agggctcaac cctgaaactg cttcgcatac tggcagactt
      121 gagggatgga gaggcttgtg gaattcctgg tgtagcggtg aaatgcattg atatcaggag
      181 gaacacccat ggcgaaggca gcaagctggc catcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012867
VERSION     KHUT01012867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           83 g           57 t
ORIGIN      
        1 tacagagagt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatatt
       61 taagtcggat gtgaaatccc tgggctcaac tcaggaattg cattcgatac tgagtttcta
      121 gagtatagta gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gcctcctgga ctaatactga cacttaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012868
VERSION     KHUT01012868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           93 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggcggcataa
       61 taagtcagat gtgaaatccc agggcttaac cctggagttg catttgaaac tgtgatgctt
      121 gagtgcagga gagggaagcg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012869
VERSION     KHUT01012869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtccgag gtgaaagcct ccagcttaac tggagaagtg ccccggatac tgattgtctc
      121 gaggagggta ggggcatctg gaactagcgg tggagcggtg aaatgcgttg atatcgctag
      181 gaactccaat ggcgaaggca aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012870
VERSION     KHUT01012870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           53 c          103 g           48 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggctcga
       61 ttagtctgct gtgaaagctc tcggcttaac cgggagaggt cggtggatac tgtcgggctt
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacctgt ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012871
VERSION     KHUT01012871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           41 c           92 g           56 t
ORIGIN      
        1 gacgtaggat gcgagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtttgat gttaaagatc ggggcctaac ctcgagagtg cattgaatac tggcagactg
      121 gagtgcagta gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgcaggcg actcgctggg ctgtaactga cgctgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01012872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012872
VERSION     KHUT01012872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggattca ttgggcttaa agggcgcgta ggcgggctcg
       61 atagtcaggg gtgaaagccc acagctcaac tgtggaagtg cctttgatac tgcgagtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012873
VERSION     KHUT01012873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           95 g           45 t
ORIGIN      
        1 gacaggggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggcctg
       61 taagtgtcct gtgaaatccc ccggctcaac cggggaactg cggggcatac tgcgggtctt
      121 gaggaaggta ggcgccgagg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg ctcggctggg cctttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012874
VERSION     KHUT01012874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacggggggt ccgaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggcttgg
       61 taagtgaacg attaaagatc aacgcttaac gttgggaatg ttgttcatac tgcctggctt
      121 gagaatatca ggggcaagcg gaactttggg tgtaggggtg aaatccgtag atatccaaag
      181 gaacaccaat ggcgaaggca gcttgctggg gtatttctga cactgagatg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012875
VERSION     KHUT01012875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          104 g           42 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggtgggt
       61 caagtccggt gtcaaatctc ccggctcaac tgggagggcg cgctggagac tggtccactg
      121 gagacaggca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg cctggactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01012876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012876
VERSION     KHUT01012876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           92 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctcc
       61 taagtcgagt gtgaaagccc tcggctcaac tgaggaagtg cattcgaaac tgggagactt
      121 gagtaccgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gccatctgga cggatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012877
VERSION     KHUT01012877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           90 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggttttt
       61 taagttagaa gtgaaaactc gaggctcaac ctcgagactg cttctaaaac tgggagactt
      121 gagtgcagga gaggaaggta gtattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatactcgt ggcgaaggcg gccttctggc ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012878
VERSION     KHUT01012878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c          101 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggcgtgta ggcggcctgt
       61 taagtcaggc gtgaaaaccc cgggcttaac ctggggactg cgcttgaaac tggcgggctt
      121 gagggcagga gaggaaagtg gaattcccgg tgtagcggtg atatgcgtag ataccgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ctgcacctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012879
VERSION     KHUT01012879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggattta ctgggtgtaa agggcacgta ggcggcccga
       61 taagctctgc ctgaaatgca ctcgctcaac gagtgcacgt ggtggaggac tgctgggctt
      121 gagggtggaa gaggccgacg gaattcccgg tgtaagggtg acatctgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gtcggctggt ccacttctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012880
VERSION     KHUT01012880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           76 g           57 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtatgca ggtggactta
       61 caagtcagaa gtgaaatccc agggcttaac cctggaattg cttttgaaac tgtcagtctt
      121 gaatcccgga gaggttaaca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgtaagcg gttaactgga cgggcattga cactcagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012881
VERSION     KHUT01012881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          103 g           40 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggattta ctgggcgtac agggtgcgta ggcggttcgg
       61 taagccctcc gggaaaactc caggctcaac ctggaaaggt cggaggggac tgccgtgcta
      121 gaggacggga gaggagcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaaggccggt ggcgaaggcg gcgctctgga acgttcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012882
VERSION     KHUT01012882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          104 g           35 t
ORIGIN      
        1 gacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggctcgc
       61 gaagcccgca gtgaaaggtc ccggcccaac cggggacgtg ctgcggggac tcgcgggctc
      121 gggcgagcta ggggtctgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acagactggg gcttggccga cgctcaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012883
VERSION     KHUT01012883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           87 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtgggcatt
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg cattcgagac tgaatgcctt
      121 gagtatggta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctttctgga ccaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012884
VERSION     KHUT01012884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c          103 g           35 t
ORIGIN      
        1 aacgtaggac gcgagcgtta tccggagtta ctgggcgtaa agggtgggca ggcggccgtg
       61 tcagatgggt gtgaaatctc ccggctcaac cgggaggggc cactcgtgac tgcacggctg
      121 gagggcagca gaggcgcgtg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcgcgctggg ctgcaactga cgctgaacca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012885
VERSION     KHUT01012885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           85 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgtta ttcggaataa ttgggcgtac agggtgcgca ggcggtgcgt
       61 taagtcaact gttaaatccc ccagcctaac tggggatcag cggtagatac tggcgcgcta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacacctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012886
VERSION     KHUT01012886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           49 c           98 g           49 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtgacg
       61 ttagtcgggc gtgaaagccc cgggctcaac ctgggatctg cgttcgatac tgtgtcgcta
      121 gagagcggaa gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctggt ccgtttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012887
VERSION     KHUT01012887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           36 c           71 g           69 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcgggttat
       61 taagtcagtg gtgaaagttt tcagcttaac tgaaaaattg ccattgatac tggtagcctt
      121 gaatttttgt gaagtaacta gaatatgtag tgtagcggtg aaatgcttag atattacatg
      181 gaataccaat tgcgaaggca ggttactaac aaacgattga cgctgatgga cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012888
VERSION     KHUT01012888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          102 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgtg
       61 caagcccggg gtgaaatcct ccagcttaac tggagaacgg cctcggggac tgtgcgtctg
      121 gagtggggta ggggtacgcg gaacactagg tggagcggtg aaatgcgttg agatctagtg
      181 gaactccggt ggcgaaggcg gcgtactgga ccccgactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012889
VERSION     KHUT01012889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggcgccg
       61 caagtgcttt gtgaaatccc atggctcaac catggaattg ctcggcagac tgcggcgctt
      121 gaggttggta ggggtcgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcgactggg ccaattctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012890
VERSION     KHUT01012890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           88 g           57 t
ORIGIN      
        1 tacgaagggg gcaagcgtta ttcggaatga ttgggcgtaa agagcgtgta ggcggttgga
       61 taagtttgat gtttaattct ctggcttaac tagaggcatg cattgaatac tgttcggcta
      121 gagtgtgaga gagggaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacgctaat ggcgaaggca gcttcctgga gcacaactga cgctgagacg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012891
VERSION     KHUT01012891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtcgggg gtgaaatcct tcagcttaac tgaagaatag ccttcgatac tggtcgtctg
      121 gaggggggta ggggcggacg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggc ggcgaaagcg gtccgctgga cccccactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012892
VERSION     KHUT01012892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           53 c          101 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggcttgg
       61 taagtcatcg gtgaaatcct ccggctcaac tggagggggt ccggcgaaac tgccaggctt
      121 gaggtcggaa gggggaagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggt ccgactctga cgctgaggtg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01012893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012893
VERSION     KHUT01012893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggcttgt
       61 caggtctgat gtgaaatctt agggctcaac cctaagcgtg catcagaaac cgactggcta
      121 gagtccggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt tgcgaaggcg gctctctgga acggtactga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01012894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012894
VERSION     KHUT01012894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c          100 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agagtgcgta ggcgggtgtg
       61 taagtgggtg gtgaaagccc tcggctaaac tgagggagga ccgctcagac tgcacgtcta
      121 gaggcggata gaggagcgtg gaattccggg tgtagcggtg aaatgtgtag agatccggag
      181 gaacaccagt ggtgcaaacg gcgctctggg tccgtactga cgctgaagca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012895
VERSION     KHUT01012895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           86 g           49 t
ORIGIN      
        1 tacggagact gcgagcgtta ctcggattca ctgggcgtaa agggtgcgca ggcggttaat
       61 tgtgtcaggt gtgaaatccc ggagcttaac tccggaactg cgcctgaaac tgattgacta
      121 gagactcgga gaggtaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaaa ggcgaaggca gcttactgga cgagttctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01012896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012896
VERSION     KHUT01012896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttggg
       61 taagtctctt gtgaaagctc ccggcttaac tgggagaggt caagggaaac tactcggcta
      121 gagtgtggta gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccatcactga cgctgatcta cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012897
VERSION     KHUT01012897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgaggggt gcgagcgtta ttcggaatta ttgggcgtac agggcgcgta gacggcatgt
       61 taagtcaact gttaaattcc ccggcctaac tggggacctg cagtagatac tggcaagcta
      121 gaggttggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccaaacctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012898
VERSION     KHUT01012898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           93 g           43 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattca ctgggcgtaa agcgcacgca ggcggcgcgg
       61 ttggtagggc atgaaacctc ccggcttaac tgggagacgc tgtcctatac cgccgcgctt
      121 gaggctggaa gagggaagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg gcttcctggt ccacgcctga cgctcaaatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012899
VERSION     KHUT01012899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           90 g           45 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattca ctgggcgtaa agggcgcgta ggcggcgcaa
       61 caagtcactt gtgaaatctc tgggcttaac ccagaacggc caagtgaaac tgttgtgcta
      121 gagtgcagaa ggggcaatcg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccaga ggtgaagacg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012900
VERSION     KHUT01012900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgg
       61 aaagttatct gtgaaatccc tgggcttaac ctgggcaggt cagataagac taacagacta
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012901
VERSION     KHUT01012901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           94 g           41 t
ORIGIN      
        1 gacggagggc gcaaacgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggagag
       61 taagtcagac gtgaaatcct ggggctcaac tccaggactg cgtttgaaac tgctatccta
      121 gagtgcagga gagggaggcg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gcctcctgga ctgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012902
VERSION     KHUT01012902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           80 g           53 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggattaa ttgggcgtaa agcgttcgta ggcggctttg
       61 taagtcattt gttaaatcct caggctcaac ctgggggctg caggtgatac tgtaaggctc
      121 gagtgtggga gaggcagatg gaatgccccg tgtagcggta aaatgcgtta atatggggta
      181 gaacaccaaa ggcgaaagca atctgctgga acacaactga cgctcatgaa cgaaagcgtg
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01012903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012903
VERSION     KHUT01012903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           85 g           47 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggcgttctcc
       61 cacgtctggc gtaaaagcac ggagcctaac tccgtgtacg tgccggaaac gaggagaatt
      121 gagtcattca gaggcaactg gaatgtcgtg tgtaggggta aaatccgttg atccacgatg
      181 gaatgccaaa agcgaaagca ggttgctggg aatgtactga cgctcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01012904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012904
VERSION     KHUT01012904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           53 c           89 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggactgg
       61 tgtgtcggat gtgaaatccc caggctcaac ctgggaactg cacccgaaac taccagcctt
      121 gagtggtgga gagggtagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccagt ggcgaaggcg gctacctgga caccaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012905
VERSION     KHUT01012905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 tacagaggcc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggatgg
       61 taagtatctt gtgaaatccc acggcttaac cgtggaactg ctgggtatac tgccgttctt
      121 gaatcagcta ggggtcgccg gaacaaatgg cggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaagaca ggcgacttgg gctgcattga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012906
VERSION     KHUT01012906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           85 g           51 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtacac tggtcatctt
      121 gagccactta ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012907
VERSION     KHUT01012907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctgac
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaattg ccctagatac tggtcagctc
      121 gagggaggta ggggcagctg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg agctgctgga tctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012908
VERSION     KHUT01012908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           96 g           38 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggttctt
       61 taagtctgat gtgaaatctc ccggctcaac cgggagcagc cattggaaac tagggaactg
      121 gagggcgaga gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggc tcgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012909
VERSION     KHUT01012909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctacg
       61 cgcgtcggat gtgaaagctc agagctcaac tctgagaggc cattcgatac ggcgtggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggag cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012910
VERSION     KHUT01012910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggctaga
       61 taggtccgct gtgaaaactc gaggctcaac ctcgagacgt cggtggaaac catctagcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaatatccgt ggcgaaggcg gctctctggt acggcactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012911
VERSION     KHUT01012911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           90 g           48 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggcctgg
       61 aaagttggga gtgaaatccc ggggctcaac tccggaattg ctctcaaaac ttctaggcta
      121 gagacttgga gaggtgggcg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt tgcgaaggcg gctcactggc caagatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012912
VERSION     KHUT01012912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           86 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggccttt
       61 taagtcagtg gtgaaagccc gcggctcaac cgcggaactg ccattgatac tggaaggctt
      121 gagtacagac gaggtgggcg gaattgaaga tgtagcggtg aaatgcttag atatcttcaa
      181 gaacaccgat tgcgtaggca gctcactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012913
VERSION     KHUT01012913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           89 g           48 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcatt
       61 taggtagggt gtgaaatccc tcggctcaac cgaggaattg cgccctaaac cgggtgacta
      121 gagtgaggca ggggtgtacg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt agcgaaagcg gtacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012914
VERSION     KHUT01012914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacgaagggt ccaagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcggatcag
       61 caagtcaggt gtgaaatctc ggggctcaac cccgaaactg cgcctgaaac tgctagtcta
      121 gaatatcgga gggggcaggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccggg ggcgaaggcg cctgcctgga cgattattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012915
VERSION     KHUT01012915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 tacgtcggga gtgaaaatcc agggctcaac tctggacgtg ctttcgatac gggcagacta
      121 gaggtattca ggggagaacg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg aattacctga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01012916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012916
VERSION     KHUT01012916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttatt
       61 caagtcaggg gtgaaatccc ggagcttaac tccggaactg cctctgaaac tagatagcta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012917
VERSION     KHUT01012917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggttaat
       61 caagttatat gtgaaattcc tgggcttaac ctgggcgctg catataatac taattagcta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacattcgt ggcgaaggcg gctttctgga ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012918
VERSION     KHUT01012918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggtggcccgt
       61 taagttcggt gtgaaagccc ctggctcaac cggggaagcg cgccggatac tgacaggcta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccctctgga cgagtactga cactgaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012919
VERSION     KHUT01012919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacgaagggt ccgagcgtag cgcggaatca ctgggcttaa agggtacgta ggcggattgg
       61 taagtatctt gtgaaagccc ccggctcaac cggggaactg ctcggtagac tgccaatctt
      121 gaggtcaaca gaggtcgtcg gaaccatagg tgtagcggtg aaatgcgtag atatctatgg
      181 gaacgccaaa ggtgaagaca gacggctggg ttgattctga cgctgaggta cgaaagcgtg
      241 ggtagcgaag agg
//
LOCUS       KHUT01012920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012920
VERSION     KHUT01012920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           83 g           53 t
ORIGIN      
        1 tacgaatgtg gcgagcgttg ttcggaatca ctgggcgtaa agagtacgta ggcggtttga
       61 caagtcagaa gtgaaagccc agggctcaac cctggaattg cttttgaaac tgttagacta
      121 gaaactcgga gaggttggcg gaattccaag tgtagaggtg aaattcgcag atatttggag
      181 gaacaccagt ggcgtaagcg gccaactgga cgagtattga cgctgaggta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012921
VERSION     KHUT01012921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           95 g           45 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggagcg
       61 cgcgtctcgg aagaaatctc acggctcaac tgtgagcctt tccgagatac ggcgcttctt
      121 gagggcagga gaggaaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agtttctggc ctgcatctga cgctgaggag cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012922
VERSION     KHUT01012922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agggcgcgca ggcggttatt
       61 taagtctgat gtgaaatctt gtggcttaac cacaagcggt cattggaaac tgaataactt
      121 gagtgcagaa gaggagagtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gctctctggt ctgcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01012923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012923
VERSION     KHUT01012923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           86 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaaa
       61 taagtcagat gtgaaattcc tgagctcaac ttggggactg cgtctgatac tgtttgacta
      121 gagtattgta gagggaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga caaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012924
VERSION     KHUT01012924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgac
       61 taagtcagat gtgaaatccc tgggcttaac ctaggaattg catttgatac tggtcagcta
      121 gagtgtggga gagggaagtg gaatttcaag tgtagcggtg aaatgcgtag atatttgaag
      181 gaacaccagt ggcgaaggcg acttcctgga ccactactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012925
VERSION     KHUT01012925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           86 g           49 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggcatgt
       61 taagttcgtt gttaaaggct tcggcctaac cgaagtattg caatgaaaac tggcaggcta
      121 gagggtgaga gagagaagtg gaattcccgg agtagcggta aaatgcgtgg atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcacaactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012926
VERSION     KHUT01012926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           86 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggtggcttga
       61 taagtctgtt gtgaaagctc ccggctcaac tgggagaggc caacagaaac tatcaggctt
      121 gagtgtggta gaggaggctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggcctctggg ccaccactga cactgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012927
VERSION     KHUT01012927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          103 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaagat
       61 taagcgtggc ttgaaagccc ccggctcaac cggggagggc ggctgcgaac tggtcttctt
      121 gagggcgaca gaggagtgtg gaattccggg agtagcggtg aaatgcgtag agatccggag
      181 gaacacccgt ggcgaaggcg gcactctggg tcgcccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012928             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012928
VERSION     KHUT01012928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agcgcgcgta ggcggcggcg
       61 taagtggggg gtgaaatcct atcggctcaa ccgtggaatc gcctctcaaa ctgcgttgct
      121 tgagggagac aggggcacgc ggaacttcca gtggagcggt gaaatgtgtt gagattggaa
      181 ggaacgccag tggcgaaagc ggcgtgctgg atctttactg acgctgaggc gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01012929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012929
VERSION     KHUT01012929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           38 c           81 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtacgta ggcggctcgg
       61 taagtctatg gtgaaagtca gcagcttaac tgttggattg ccaaagatac tgccttgctt
      121 gagtatagtc gaggtaggtg gaattccata tgtagtggtg aaatgcttag atatatggag
      181 gaacgccgat aacgaaggta acttactagg ctataactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012930             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012930
VERSION     KHUT01012930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           95 g           40 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtgtccg cgtgaaatcc cacggctcaa ccgtggaacg gccgggcata ctggcgacct
      121 tgaggtgctc aggggcgagc ggaacaaacg gtggagcggt gaaatgcgta gatatcgttt
      181 ggaacgccga cggtgaaaac agctcgctgg gagcatcctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01012931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012931
VERSION     KHUT01012931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgt
       61 caagtcaggg gtgaaatcct gcggctcaac cgcagaagtg cctttgatac tgacggtctc
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012932
VERSION     KHUT01012932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           99 g           38 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggg
       61 aaagtcaggg gtgaaagccg gcagctcaac tgtcgaagtg cctctgatac tctccgcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012933
VERSION     KHUT01012933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           49 c           78 g           51 t
ORIGIN      
        1 tacagaagtc ccaagcgttg ttcggattca ctggacgtaa aggatgcgta ggtggctgaa
       61 taagtctgat gtgaaatctc cgagctcaac tcggaaatgg cattggatac tattcggctc
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaat ggcgaaggcg agtctctgga cgactcctga cactgaggca cgaaagctaa
      241 aaaagcaaac agg
//
LOCUS       KHUT01012934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012934
VERSION     KHUT01012934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgtgg
       61 aatgtcagat gtgaaatccg gtggctcaac caccgaaatg catccgaaac ttccatgctt
      121 gagtgctgta ggggagtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgt ggcgaaggcg ggactctggg cagcaactga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012935
VERSION     KHUT01012935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          102 g           41 t
ORIGIN      
        1 aacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggccgtt
       61 taagtcctgt gtgaaagacc ccggcttaac cgggggaggt catgggaaac tggacggctt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggtaactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012936
VERSION     KHUT01012936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggccctt
       61 taagtcagat gtgaaagccc gcggcttaac cgcggaactg catttgaaac tggagggctt
      121 gagtgtcgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actacctgga cgataactga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012937
VERSION     KHUT01012937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatca ctgggcgtaa agcgcacgca ggtggtctct
       61 taagtccact gttaaatcac ggggctcaac cccggaggtg cagcggatac tgagggactg
      121 gagactggta gaggctagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctagctggg ccagttctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012938
VERSION     KHUT01012938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           89 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatca ttgggcgtaa agggcgcgta ggcggtctga
       61 taagtcaaat gtgaaaatcc agggcttaac tctggaagtg cgtttgaaac tgtgagacta
      121 gagtacagga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacatcgat ggcgaaggca gctttctggc ctgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012939
VERSION     KHUT01012939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           87 g           56 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaataa ctgggcgtaa agagcgcgta ggcgggtata
       61 taagttattt gtgaaagccc cgggcttaac ctgggaattg caggtaaaac tgtatatctt
      121 gaatactgca ggggatggtg gaattgctgg tggagaagtg aaatttatag agatcagcag
      181 gaacacctga ggcgaaggcg gccatctggg catgtattga cgctgtggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012940
VERSION     KHUT01012940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          102 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgca ggcggcccgt
       61 tccgtcccgt gtgaaagcct ccggctcaac cggaggaggt cgcgggatac tggcgggctt
      121 gaggaaggca gaggatggta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatgccagt ggcgaaggcg gccatctggg ccttctctga cgctgagacg cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012941
VERSION     KHUT01012941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           91 g           45 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattga ctgggcgtaa agcgtatgta ggtggtacgg
       61 caggtggggt gttacatctc caggcttaac ctggagccgc cgctccaaac cgccgcactc
      121 gagtcgtgca gaggttggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacctaa agcgaaagca gccaactggg cacgtactga cactgaggta cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012942
VERSION     KHUT01012942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          101 g           41 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcacgca ggcggtcagg
       61 taagtgtcgt gtgaaagcgg cccgcttaac gggtcgaggc catggcagac tgcccggctg
      121 gaggtcgtga gaggtccgtg gaattgccgg tgtagtggtg atatgcgtag atatcggcag
      181 gaacacccaa ggggaagcca gcggactggc acgatgctga cgctgaggtg cgatagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01012943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012943
VERSION     KHUT01012943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          106 g           31 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agcgcgcgca ggcggtcaca
       61 tatgtgcccg gtgacactcc acggctcaac cgtggacggc ccgggtacac agtgggactg
      121 gaggcaggga gagggtcgtg gaatgcccgg tggagcggtg aaatgcgtag agatcgggcg
      181 gaacaccagc ggcgaaggcg gcgacctggc cctgaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012944
VERSION     KHUT01012944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgagcgca ggcggatcag
       61 taagtcaggt gtgaaatctc gaggctcaac ctcgaaattg cgcctgaaac tactagtcta
      121 gaatgttgga ggggataggg gaatttcacg tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccgga ggcgaaggcg cctatctgga caactattga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012945
VERSION     KHUT01012945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           91 g           50 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgtgta ggcggcccga
       61 tcagtagggt gtgaaaggct tcggctcaac cggagcactg cgctctagac tttcgggctc
      121 gagcaagaca ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccggt ggcgaaagcg gcacactggg tcttgtctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012946
VERSION     KHUT01012946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           55 c          107 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcatcg
       61 caagtcggcc gtgaaatctc ccagctcaac tgggaggggt cgggcgagac tgcggagctc
      121 gaggtgggta gagggcagtg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctgcctggg cctggcctga cgctgaggcg cgaaagcgtg
      241 gggagcaatc cgg
//
LOCUS       KHUT01012947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012947
VERSION     KHUT01012947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           98 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgctg
       61 ctagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgcttgatac ggcagtgcta
      121 gaggatggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012948
VERSION     KHUT01012948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggtctgg
       61 tcagtcggac gtgaaagccc ggggctcaac cccggaagtg cattcgatac tgccaggctg
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012949
VERSION     KHUT01012949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           91 g           50 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggatatt
       61 taagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tggatatcta
      121 gagtacgaga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacgtcaat ggcgaaggca gccacctggc tcgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012950
VERSION     KHUT01012950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacggaggtg gcaagcgttg ctcggattta ctgggtgtaa agggcacgta ggcggcccta
       61 taagccctgc ctgaaatgca ctggctcaac catggcatgt ggcaggggac tgcagggctt
      121 gagagtggaa gaggcgagcg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctcgctggt ccatatctga cgctgaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012951
VERSION     KHUT01012951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           80 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctttt
       61 taagtcagtg gtgaaatcca acagctcaac tgttgaactg ccattgatac tggagagctt
      121 gagtacagac gaggtaggcg gaatttatgg tgtagcggtg aaatgcatag ataccataaa
      181 gaacaccgat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012952
VERSION     KHUT01012952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           48 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ctgggcgtaa agggttcgta ggtggcttgc
       61 taagtcagac gtgaaatccc tcggcttaac cggggaattg cgtctgaaac tggcgagctt
      121 gagtgcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcaactga cactgaggaa cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01012953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012953
VERSION     KHUT01012953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           97 g           43 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattca ctgggcgtaa agcgcgtgta ggcggtcggt
       61 caagtcggct gttaaagccc ctggcttaac tgggggaggc cagccgagac tgctcgactt
      121 gagggtagta gaggagagtg gaactcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccagt ggcgaaggcg actctctggg ctacacctga cgctgagacg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012954
VERSION     KHUT01012954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtcta ggctgtttgc
       61 taagtttgtt gtgaaagccc ctggctcaac cagggatgtg caatggatac tggcgaactt
      121 gaataatgga gaggtgggcg gaattccggg tgtagcagtg aaatgcgtag atatccggag
      181 gaacaccaga ggcgaaggcg gctcactggc catttattga cgctgaagcg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01012955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012955
VERSION     KHUT01012955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agcgcacgta ggcggctctt
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaactg ccttcgatac tgtagagctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggtg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012956
VERSION     KHUT01012956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacagaggag gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggtctgg
       61 taagtacttt gtgaaatacc tgggctcaac ctgggaactg cttggtatac tgccagactc
      121 gaggcggata ggggtgcctg gaactctagg tggagcggtg aaatgcgtag atatctaggg
      181 gaacgccaga ggcgaaagcg gggcactggg tccgatctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01012957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012957
VERSION     KHUT01012957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           85 g           48 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgttctgc
       61 cacgtctggc gtaaaatttc ggggctcaac cccgaatgcg tgctggaaac gagcggaatc
      121 gagtcattca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaggca ggatgctggg aatgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01012958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012958
VERSION     KHUT01012958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           95 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcgtgt
       61 cgcgtcgaaa gtgaaaatcc aacgctcaac gttggacttg ctttcggtac gggcatgctt
      121 gagtgtggta ggggagtctg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggactctggg ccattactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012959
VERSION     KHUT01012959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c          104 g           38 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggccgat
       61 caagtcccgt gtgaaaggcc acggcttaac cgtggagggc catgggaaac tggttggctg
      121 gagggccgga gagggtggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cggaccctga cgctgaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012960
VERSION     KHUT01012960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           93 g           49 t
ORIGIN      
        1 gacgggagat gcgagcgttg ttcggaatca ttgggcttaa agcgtgtgta ggcggctcgg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtctac tgccgggctc
      121 gaggttggta gaggtgcctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgcccgt ggcgaaagcg gggcactggg ccaatcctga cgctgagaca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012961
VERSION     KHUT01012961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           75 g           62 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggtctat
       61 taagtcagtg gtgaaatctc tcggcttaac tgagaaattg ccattgatac tggtagactt
      121 gagtgtagtt gccgttggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctaacgaaa ctataactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01012962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012962
VERSION     KHUT01012962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           94 g           43 t
ORIGIN      
        1 gacgaaccgg acgaacgtta ttcggaatta ctgggcttaa agagtgcgta ggcggcctgg
       61 caggttgggt gtgaaatccc tcggctcaac cgaggaactg cgctcaaaac cgccaggctg
      121 gagggagaca ggggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcactggg tcttctctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012963
VERSION     KHUT01012963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           92 g           50 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggttagg
       61 taagtgagat gtgaaaggct ccggctcacc cggagacatg catctcatac tgcttagctc
      121 gagtacagga gaggaaacga gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg cgtttctggc ctgttactga cgctgatgcg cgatagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012964
VERSION     KHUT01012964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c          101 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggccgtt
       61 taagtcctgt gtgaaaggtc ccggcttaac cgggaaaggt catgggaaac tgggcggctt
      121 gagtgccgga gagggttgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaggcg gcaacctgga cggtgactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01012965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012965
VERSION     KHUT01012965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 aaagtctgat gtgaaatctc caggctcaac ctggaaatgg cattggaaac taccttgctc
      121 gaggagtgga ggggggactg gaatgctcgg tgtagcagtg aaatgcgtag atatcgagcg
      181 gaacaccagt ggcgaaggcg agtccctgga cacttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01012966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012966
VERSION     KHUT01012966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           65 c          109 g           29 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtcccg
       61 caggtcacct gtgaaagccc ccggctcaac cgggcggagg cgggtgaaac cgcgggactg
      121 gagggcggca gagggtcgtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgc ggcgaaggcg gcgacctgga ccgaccctga cgctgaggcg cgaaggccgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012967
VERSION     KHUT01012967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           97 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggacgcg
       61 tcagtcaggg gtgaaatccc ggggctcaac ctcggaacgg cctttgatac tgcgggtctg
      121 gagtccggaa gaggttggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga ggcgaaggcg gccaactggt ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012968
VERSION     KHUT01012968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           40 a           66 c          114 g           33 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccgcg
       61 agagcccggc gtgaaagccc ccggctcaac cgggggaggc cgtcggggac ctcgcggctg
      121 gaggccggta ggggcgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggt ggcggaggcg gctcgctggg ccggtcctga cgctcgggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01012969             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012969
VERSION     KHUT01012969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c          104 g           37 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggccgct
       61 caagtggtgt gtgaaatcgc ccggctcaac cgggcgcggt caggccagac tgaggggcta
      121 gagtgcggga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc ccgacactga cgctgagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012970
VERSION     KHUT01012970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           95 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgac
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaatag cctttgatac tggtcgtctg
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01012971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012971
VERSION     KHUT01012971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           86 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttata
       61 taagttagat gtgaaatccc cgggcttaac ctgggaacgg catttaatac tgtatggcta
      121 gagtagaata gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ttcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012972
VERSION     KHUT01012972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           55 c          105 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggcccgca ggtggtttcg
       61 tgcgtcgcgc gtgaaatacc ccggctcaac tggggggtgt cgtgcgatac ggcgagactg
      121 gaggccagta gggggatgcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaacaccagc ggcgaaggcg gcatcctgga ctggtcctga cactcagggg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01012973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012973
VERSION     KHUT01012973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c          103 g           42 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggcggat
       61 caagtcaggc gtgaaagccc cgggctcaac ctgggaggtg cgcttgggac tggtctgctt
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012974
VERSION     KHUT01012974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           83 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggaaatt
       61 taagtcgatt gtgaaatccc cgggctcaac ctgggaactg cagtcgagac tggatatcta
      121 gagtatagta gaggaaagta gaattcccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaataccagt ggcgaaggcg gctttctgga ctaatactga cactgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012975
VERSION     KHUT01012975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           87 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggattgc
       61 taagtcagat gtgaaattcc tgggcttaac ctgggaactg catttgaaac tggtaatcta
      121 gagtgcggga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ccgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012976
VERSION     KHUT01012976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtttggg gtgaaatcca ccggctcaac cggagaagtg cctcgaatac tggcggtctg
      121 gagggaggta ggggcgtgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012977
VERSION     KHUT01012977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 gacgagggat cctagcgttg ctcggaatta ttgggcgtaa agagggcgta ggtggctttg
       61 taagtcaggt gtgaaagccc cgggctcaac ccgggaagtg catttgatac tgcgaagctt
      121 gagtgttgga gaggtcatcg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg ggtgactggc caaacactga cactgaggcc cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01012978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012978
VERSION     KHUT01012978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtttca
       61 tttgtctggg gtgaaagcct ttggcttaac caaagaagtg ccccggaaac ggtggaacta
      121 gagtgctaga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg acgacctgga tagcaactga cactcaacta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012979
VERSION     KHUT01012979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcaaag
       61 taagtcgggt gtgaaatcct ggggctcaac cccggaactg cactcgatac tgctctgctc
      121 gagtactgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agtctctgga cagttactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01012980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012980
VERSION     KHUT01012980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcttg
       61 taagtcccgt tgtgtaagac cggggctcaa ccccgggttg caagggaaac tgcaggactt
      121 gagtgcggga gagggaagcg gaattccatg tgtagcggtg aaatgcgtag agatatggag
      181 gaacaccagt ggcgaaggcg gcttcctggt ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012981
VERSION     KHUT01012981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           83 g           55 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgtgta ggaggttagg
       61 tcagtcgggc gtgaaatctc accgcttaac ggtgaaactg cgttcgatac tacttgacta
      121 gaggatcgga gggggtatcg gaattcatgg tgtagcagtg aaatgcatag atatcatgag
      181 gaacaccggt ggcgaaagcg gatacctgga agattcctga ctctgaaaca cgaaagctag
      241 gagagcaaac ggg
//
LOCUS       KHUT01012982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012982
VERSION     KHUT01012982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           95 g           49 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatga ctgggtgtaa agggcgcgta ggcggtgttt
       61 taagtgaggc gtgaaagccc tgggctcaac ccaggaggtg cgtttcatac tggaatacta
      121 gagtgcgaga gaggaaagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccaga ggcgaaggcg gctttctggc tcgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012983
VERSION     KHUT01012983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggttgc
       61 caagtctgtg gtgaaatcct ccagcttaac tggagaacgg ccatggatac tggcgacctc
      121 gaggggggca ggggcttgcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg gcgagctgga ccctttctga cgctgaagca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01012984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012984
VERSION     KHUT01012984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctcg
       61 caagttgggt gtgaaagccc tcggcttaac cgaggaagtg cattcaaaac tacgagactt
      121 gaatactgga gagggttgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcaacctgga cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012985
VERSION     KHUT01012985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           54 c           82 g           43 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggtctaa agcgtctata ggcggctttt
       61 caagtccgcc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaacctg
      121 gagtacggta ggggcagagg gaatttccgg tggagcagtg aaatgcattg agatcggaaa
      181 gaacaacaac ggtgaaagca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01012986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012986
VERSION     KHUT01012986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggaccta
       61 taagtcgatt gtgaaatccc tgggctcaac ccgggaattg cagtcgaaac tgcaggtctt
      121 gagttctcaa gaggatggtg gaattcctgg agtagtagtg aaatacgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gccatctggt gagacactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01012987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012987
VERSION     KHUT01012987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggtgtaa agggcgtgta ggcggggagg
       61 caagtcagat gtgaaattcc ggagctcaac tccggcgctg catctgaaac tgcttctctt
      121 gagtactgga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggag
      181 gaacaccagt ggcgaaggcg actttctgga cagtaactga cgctgaggcg cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012988
VERSION     KHUT01012988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggtggttatc
       61 taagtctggt gttaaagccc ggagctcaac tccggcatgg cattggaaac tggatggcta
      121 gagtgtggaa gaggtaactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaagcg agttactggt ccactactga cgctgaggaa cgaaagccag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01012989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012989
VERSION     KHUT01012989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttttg
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaattg catccgatac tgcttaacta
      121 gagtatgcta gagggaagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccaat ggcgaaggca acttcctggg gcaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012990
VERSION     KHUT01012990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           85 g           59 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggcgggtttg
       61 taagtcagtg gtgaaatatt gtcgcttaac gataaggttg ccattgatac tgcaggtctt
      121 gagtttggtt gaggtaggcg gaatgtgcag tgtagcggtg aaatgcatag agattgcaca
      181 gaacaccgat tgcgaaggca gcttactaag ccaaaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01012991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012991
VERSION     KHUT01012991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           80 g           56 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtgtgta ggcggatttg
       61 ttagtcagat gtcaaagacc ggggctcaac cccgggaagg catttgaaac ggcaagtcta
      121 gagaatgtga gagattgctg gaattcatgg tgtagtagtg aaatgcgttg atatcatgag
      181 gaacactcaa ggcgaaggca agcaattggc acatttctga cgctcagaca cgaaagcgtg
      241 gggagcgaat ggg
//
LOCUS       KHUT01012992             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012992
VERSION     KHUT01012992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 gacagaggag gcgagcgttg ttcggaatca ttgggcttaa agcgcgtgta ggcggtcccg
       61 taagtgtctt gtgaaatccg gtggctcaac catcgaactg ctgggcatac tgcgggactt
      121 gaggttggta gaggtgcctg gaactcttgg tggagcggtg aaatgtgtag atatcaagag
      181 gaacgccgga ggcgaaagcg gggcactggg ccaaatctga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01012993             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012993
VERSION     KHUT01012993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggaatta ctgggcgtaa agcgcatgca ggcggttctt
       61 taagtcggac gtgaaagctc ctggctcaac taggagaggc cgttcgaaac tgaaggactt
      121 gaggatggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccatacctga cgctcagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01012994             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012994
VERSION     KHUT01012994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           60 c           94 g           39 t
ORIGIN      
        1 tacgagaggc ccgagcgtta atcggaatca ctgggcttaa agcgtgcgca ggcggatcgg
       61 caggcatcct gtgaaatccc acggctcaac cgtggaacag cggggtgaac cgccgatctt
      121 gaggcaggta ggggctgatg gaaccatggg tggagcggtg aaatgcgtag atatccatgg
      181 gaacgccgat ggcgaaggca gtcagctggg cctgtcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01012995             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012995
VERSION     KHUT01012995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggaccat
       61 caagtcgggg gtgaaatcct ggagcttaac tccagaactg ccttcgatac tgttggtctt
      121 gaggtcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgaatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012996             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012996
VERSION     KHUT01012996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           89 g           51 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccgatatta ttgggcgtaa agagtgcgta ggcggtttgg
       61 taagacaatg gttaaagacc cgagctcaac tcggggagtg ccgttgttac tgccagactc
      121 gacgatagca ggggcaagtg gaattctcgg tgtaggggtg aaatccgttg agatcgagaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctatatgtga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01012997             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012997
VERSION     KHUT01012997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           84 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggtgtgt
       61 taagtctgtt gttaaaggct ccagcctaac tggagacatg cagtggaaac taacatgcta
      121 gagggcggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccgcttctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01012998             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012998
VERSION     KHUT01012998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           37 c           87 g           59 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttgggt gtgaaagccc tgagcttaac ttggggattg cgcttaatac tgttgagcta
      121 gagtactgta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01012999             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01012999
VERSION     KHUT01012999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttcat
       61 taagttatct gtgaaattcc cgggcttaac ctgggcgggt cagataatac tggtggacta
      121 gagtatagga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctgcctggc ctaatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013000             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013000
VERSION     KHUT01013000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 tacaggaggc ccgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcgggtcag
       61 taagagtgtc gtgaaatccc tcggctcaac cggggaactg cggcgcagac tgctgatctt
      121 gaggaagcta ggggtcgctg gaacgctcgg tggagcggtg aaatgcgttg atatcgagcg
      181 gaacgccgat ggtgaagaca ggcgactggg gctttcctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013001
VERSION     KHUT01013001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          108 g           35 t
ORIGIN      
        1 gacggagggc gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggaagag
       61 gaagtcggga gtgaaagacc ggggctcaac tccggggcgg cttccgatac tcctgttctg
      121 gggaccggta gaggcgggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013002
VERSION     KHUT01013002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacagagatg gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggagtga
       61 tgggtgcctt gtgaaatccc tcggctcaac cggggaatgg ctcggcaaac cggcactctt
      121 gaggcaagta gaggtaactg gaaccattgg tggagcggtg aaatgcgtag agatcaatgg
      181 gaacgccggt ggcgaaagcg agttactggg cttgttctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013003
VERSION     KHUT01013003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           90 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatca ttgggcgtaa agggcgcgta ggcggtttag
       61 taagtttggt gtgaaatccc ggggcttaac tccggaagtg cattgaatac tgctagacta
      121 gagtgtagga gaggatggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacagt ggcgaaggcg gccatctgga ctacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013004
VERSION     KHUT01013004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           98 g           40 t
ORIGIN      
        1 aacgtaggag acaaacgtta tccggagtta ctgggcgtaa agggcgtgca ggtggctgct
       61 taagttcggc gtgaaagcct ccggctaaac cgggggaggt cgtcggaaac tggacagcta
      121 gagggtgata gaggcgggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acccgctggg tcacacctga cactgagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013005
VERSION     KHUT01013005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           96 g           44 t
ORIGIN      
        1 tacagggggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctggt
       61 caagtggaag gtgaaatccc tcggctcaac cgaggaactg cctttcaaac tgatcagctt
      121 gagtccggga gaggcgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcgctgga ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013006
VERSION     KHUT01013006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           75 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcggatatt
       61 taagtcagcg gtgaaatctc cgggctcaac ccggaaactg ccattgatac tatttatctt
      121 gaattctgtt gaggtaggcg gaatacatca tgtagcggtg aaatgcttag agatgatgta
      181 gaacaccgat tgcgaaggca gcttactaaa cagctattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013007
VERSION     KHUT01013007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           93 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcgaaatta ctgggcgtaa agggtgcgta ggtggcgaaa
       61 taagttactt gtgaaagtcc tgggcttaac ccaggttgga cgagtgatac tgttttgctt
      121 gagtacagga gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcggt ggcgaaggcg gctgcctggt ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013008
VERSION     KHUT01013008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttttg
       61 taagtcagat gtgaaatccc ggggcttaac tccggaagtg catttgaaac tgcaaggcta
      121 gagtgcagta gaggttggtg gaattcctag tggagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctgcaactga cgctgaggcg cgagagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013009
VERSION     KHUT01013009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           85 g           46 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatga ctaggcgtaa agggcaggta ggcggtacgg
       61 caagtctgtt gtgaaagctc ctggcttaac tgggagaggc caacagaaac tgccgaactt
      121 gagtatggga gaggatactg gaattcccgg tgtagcggtg aaatgcgctg atttcgggag
      181 gaacaccaac ggcgaaagca agtatctgga ccattactga cgctcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013010             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013010
VERSION     KHUT01013010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           99 g           46 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggctagt
       61 tttgtcgagt gtgaaagccc cgggcttaac ctgggaactg cgctcgaaac gggctggcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gagcaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013011
VERSION     KHUT01013011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtgggccat
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaagac tgatggtcta
      121 gagtcggaga gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcgacctggt tccagactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013012
VERSION     KHUT01013012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           82 a           44 c           78 g           49 t
ORIGIN      
        1 tacaggggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgca ggtggttttg
       61 aaagtcgatt gtcaaaaacc aaggcttaac cttgggccgg caatggaaac ttcaagacta
      121 gagtatggta gaggtagagg gaattaccgg tgtaacggtg aaatgtgcag atatcggtaa
      181 gaacaccaaa ggcgaaagca ctctactggg ccaaaactga cactcagaga cgaaagctag
      241 gggagcgaat agg
//
LOCUS       KHUT01013013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013013
VERSION     KHUT01013013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           89 g           53 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcggcgcta
       61 caagtgcctt gtgaaatccc atcgcttaac gatggaattg cttggtagac tgtagtgctt
      121 gaggttggta ggggctgtcg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca gacagctggg ccaattctga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013014
VERSION     KHUT01013014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           90 g           45 t
ORIGIN      
        1 tacgaagggc ccgagcgttg cgcggaatta ctgggcttaa agggtgcgta ggcggacttg
       61 caagtgtctt gtgaaatacc acggcttaac cgtggaactg ctcggcaaac tacaagtctt
      121 gagcccgtca ggggtcgtcg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca gacgactggg acggcgctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013015
VERSION     KHUT01013015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           56 c           87 g           44 t
ORIGIN      
        1 tacagagggc ccaagcgtta atcggaatca ctgggcttaa agggtgcgca ggcggacctg
       61 taagtgcttt gtgaaatccc actgctcaac ggtggaattg ctcggcaaac tacaggtctt
      121 gagcccgaca ggggtcatcg gaacgctagg tggagcggtg aaatgcgtag atatctagcg
      181 gaacgccaaa ggtgaagaca gatgactggg tcggcgctga cgctcaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013016
VERSION     KHUT01013016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          100 g           40 t
ORIGIN      
        1 tacggagggg gctagcgttg tccggaatca ctgggcgtaa agcgcgcgta ggcggcttgg
       61 taagtcgggt gtgaaagccc ggggctcaac cccggatgcg catccgagac tgccaggctt
      121 gagtccgaga gagggcggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccgcctggc tcggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013017             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013017
VERSION     KHUT01013017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           90 g           55 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggcggggagt
       61 caagtcagtt gtgaaaagcc gcggcccaac cgtggtcgtg cagttgaaac tggttctctt
      121 gagtgcgcac gaggacggtg gaattcgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gccgtccgga gcgttactga cgctgaggct cgaaggtgcg
      241 ggtatcaaac agg
//
LOCUS       KHUT01013018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013018
VERSION     KHUT01013018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggaggcttgg
       61 aaagtcaggt gtgaaatctc gccgcttaac ggcgaaatgg cgcttgatac tcccgagcta
      121 gaagatcgga ggggagaacg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaac ggcgaaggca gttctctgga cgattcttga ctctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013019
VERSION     KHUT01013019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           91 g           50 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccgtt
       61 caagtagggt gtgaaaggct ccggctcaac cggggcattg cgctctaaac tgttcggctc
      121 gagtgagata ggggtacgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacaccggt ggcgaaagcg gcgtactggg tctcaactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013020
VERSION     KHUT01013020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           91 g           53 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccggagat
       61 taagcgtgtt gtgaaatgcc gcggctcaac cgtggcactg cagcgcgaac tggttttctt
      121 gagtgcgcgc gacgcaggcg gaattcgtgg tgtagcggtg aaatgcttag atatcacgag
      181 gaaccccgat tgcgaaggca gcttgcggga gcgcaactga cgctgaagct cgaaggtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01013021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013021
VERSION     KHUT01013021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 gacgtaggtg gcaaacgtta tccggaatca ctgggcgtaa agggtccgca ggcggttcaa
       61 caagtcggac gtgaaagctc gcggctcaac tgcgagaagc cgttcgaaac tgttgaactt
      121 gaatccggca gaggtcgatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccaag agcgaaggca gtcggctggg ctgagattga cgctgaggga cgaaggcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013022
VERSION     KHUT01013022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           92 g           50 t
ORIGIN      
        1 tacgtactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccgac
       61 taagcagggt gtgaaatccc ccggctcaac cggggaattg cgctctggac tggttggctt
      121 gagtaaggta ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggc agcgaaagcg gcacactggg ccttaactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013023
VERSION     KHUT01013023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcagaatca ctgggcttaa agcgtgtgta ggcggaatgc
       61 taagtacctt gtgaaatcct gcggctcaac cgcagaactg ctgggtatac tggtattctt
      121 gagccactta ggggcagtcg gaacagatgg tggagcggtg aaatgcgtag atatcatctg
      181 gaacgccaaa ggtgaaaaca gactgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013024
VERSION     KHUT01013024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 taagtgtgat gtgaaagccc agggcttaac cttggaactg catcacatac tgtcaggcta
      121 gagtacgaga gaggggggta gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaataccagt ggcgaaggcg gccccctggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013025
VERSION     KHUT01013025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           98 g           48 t
ORIGIN      
        1 tacggggggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgg
       61 tgtgtcaaag gtgaaatccc tcggctcaac tgaggaattg cctttgaaac taccgggctt
      121 gaggccggga gggggtagcg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013026
VERSION     KHUT01013026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           97 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggaccgg
       61 tcagtcagag gtgaaatccc ggggctcaac ctcggaattg cctttgatac tgttggtctt
      121 gaggacggga gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctgga ccggatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013027
VERSION     KHUT01013027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           94 g           49 t
ORIGIN      
        1 tacgaatcgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtccgag gtgaaatccc acagctcaac tgtggaactg ccccggatac tggtcgtctt
      121 gagggaggta ggggcgtgtg gaacgtttgg tggagcggtg aaatgcgttg atatcaaacg
      181 gaactccggt ggcgaaggcg acacgctggg cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaag agg
//
LOCUS       KHUT01013028             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013028
VERSION     KHUT01013028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggcggcctgt
       61 taagtcagtt gtgaaatccc acggctcaac cgtggaattg caattgaaac tggcgggctt
      121 gagtgcagga gaggttggta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gccaactggc ctagcactga cgctgagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013029
VERSION     KHUT01013029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           56 c          106 g           40 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggttgga
       61 tgcgccggtg gtgaaagcgc agggctcaac cctgcgtcgg ccatcgggac ggtccgactg
      121 gagcactgta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013030
VERSION     KHUT01013030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           59 c          107 g           37 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtccgta ggcggctcca
       61 tgcgtcggtg ctgaaagcgc caggcttaac ctggcgaggc gtgccgatac gatggggctt
      121 gaggcaggca gagggtggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgccggt ggcgcaagcg gccacctggg cctgacctga cgctgaggga cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013031
VERSION     KHUT01013031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgagtgta ggcggcttcg
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaattg cattcgatac tgcgcggcta
      121 gagtctgata gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg gcttcctgga tcaagactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013032
VERSION     KHUT01013032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcggc
       61 caagtctggg gtgaaatcct gcagcttaac tgtagaagtg cctgagatac tggctggctc
      121 gagggaggta ggggtctgtg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg acagactgga ccttttctaa cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013033
VERSION     KHUT01013033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcttaa
       61 caagtcagag gtgaaatgcc agggctcaac ccgggaactg cctttgaaac tgttaagctt
      121 gagactcgga ggggatagcg gaatacctaa tgtagcggtg aaatgcgtag atattaggtg
      181 gaacaccggt ggcgaaggcg gctatctgga cgagtactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013034
VERSION     KHUT01013034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           78 g           62 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattta ctgggcgtaa agggtgtgta gacggcacta
       61 taagttcgtt gttaaatctt tcggcctaac cgaaagcttg cgatgaaaac tattttgctt
      121 gagagtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013035
VERSION     KHUT01013035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           79 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ttgggtttaa agggtccgca ggcggactag
       61 taagtcagtg gtgaaatctc tcagcttaac tgagaaactg ccattgatac tgctagtctt
      121 gaattcggtc gaagtgggcg gaatgtgtca tgtagcggtg aaatgcatag atatgacaca
      181 gaacaccgat agcgaaggca gctcactagg cctgaattga cgctcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013036             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013036
VERSION     KHUT01013036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           57 c           97 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggtttaa agagcgcgta ggcgggtcgg
       61 cgcgtcggaa ctgaaatccc ccggcttaac cggggaacgg gattcgatac ggctggcctc
      121 gaggtgagaa gggggacctg gaacgtccgg tggagcggtg aaatgcgttg agatcggacg
      181 gaacgccaat ggcgaaagca aggtcctggg ctcataactg acgctgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013037             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013037
VERSION     KHUT01013037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           95 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta atcggattca ttgggcttaa agggcgcgta ggcggcggcg
       61 taagttcgtg gtgaaatccc atcggctcaa ccgtggaatt gcttcgaata ctgcgtcgct
      121 cgagggagat aggggaccgc agaactgccg gtggagcggt gaaatgtgtt gatatcggcg
      181 ggaatgccag tgccgaaagg ggcggtctgg acctcttctg acgctgaggc gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013038             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013038
VERSION     KHUT01013038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           62 c           97 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggggcgg
       61 aacgtcggat gctgaaagcc cccggctcaa ccggggaagt ggcaccgata cgaccgccct
      121 cgagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttttctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013039             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013039
VERSION     KHUT01013039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgcgca ggcggatgga
       61 gcaagtctgg agtgaaatct cgccgctcaa cggcgaggct gctttggata ctgcacatct
      121 agggaccggt agaggctggt agaattgctg gtgtagcggt ggaatgcgta gatatcagca
      181 agaatacccg tggcgaaggc ggccagctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013040
VERSION     KHUT01013040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           84 g           55 t
ORIGIN      
        1 tacagaggcc tcgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggtgacg
       61 taagtcagat gtgaaagccc tgggcttaac ccgggaagtg catttgatac tgcgtcgctc
      121 gagtacgaga gaggaaagcg gaatttccag tgtaggagtg aaatccgtag atattggaag
      181 gaataccgat ggcgaaggca gctttctggc tcgtaactga cactgagatt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013041             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013041
VERSION     KHUT01013041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           89 g           51 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 aaagtcaggt gtgaaacctc caggctcaac ctggagacgc cacttgatac ttctatggct
      121 tgagttcggt aggggtagac ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccga tggcgaaggc agtctactgg gccgatactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01013042             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013042
VERSION     KHUT01013042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           80 g           64 t
ORIGIN      
        1 cacgggggct ccaagcatta tccggtttta ttgggcgtaa agagttctgt aggtggtgat
       61 gtaagttatg ggttaaattt tccggcttaa ccgggaactt gcttgtaata ctgcattgct
      121 agaggcaaac aggggaaagc agaacgcatg gtgtagtggt aaaatacgtt gatatcatgc
      181 ggaataccga aggcgtaggc agctttctgg gtttgtcctg acactgagag acgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01013043             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013043
VERSION     KHUT01013043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           86 g           60 t
ORIGIN      
        1 tacgagggat gcaagcgtta tccggattta ttgggtgtaa agcgtttcgt aggcgttttc
       61 gtaagttatc tttcaaagac cggggctcaa cctcggaaag gggggtaata ctgcgggaat
      121 tgaagaatgt cggggctact ggaacttatg gtgtaggagt gaaatccgtt gatatcataa
      181 ggaacaccaa gggcgtaggc gggtagctag gcattatttg acgctgagga acgacagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01013044             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013044
VERSION     KHUT01013044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           94 g           46 t
ORIGIN      
        1 tacggaaggg gcgagcgttg ttcggattca ttgggcgtaa agcgcgtgta ggcggtctgg
       61 tcagtcgaag gtgaaatcgc tacggctcaa ccgagcaatt gccctcgaaa ctgccagact
      121 tgagtgcgga agaggtgagt ggaattccca gtgtagcggt gaaatgcgta gatattggga
      181 ggaacaccag tggcgaaggc ggctcactgg tccgcaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013045
VERSION     KHUT01013045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           96 g           40 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcatgca ggcggccgag
       61 caagttggat gtgaaagctc ccggctcaac tgggagagga cgtacaagac tgcttggctc
      121 gaggacggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gcctcctggg ccggacctga cgctgagatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013046
VERSION     KHUT01013046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcttgta ggcggttttt
       61 caagtctcgt gtgaaagctc caggctcaac ctggaaagtg cgcgagaaac tgggagactt
      121 gagtgtcgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cgagcactga cgctgagaag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013047
VERSION     KHUT01013047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaacgg cttctgatac tggcaggctg
      121 gagggaggta ggggcggatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013048
VERSION     KHUT01013048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           63 c           89 g           44 t
ORIGIN      
        1 taccagctcc tcgagtggtc ggggcgatta ttgggcctaa agcatccgta gccggttccg
       61 taggtctcct gcaaaatcca gtcgctcaac gattggcccg caggagatac cgcgggacta
      121 ggaggcggga gaggtggacg gtactccacg tgtaggggta aaatcctttg atccgtggaa
      181 gaccaccagt ggcgaaggcg gttcaccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013049
VERSION     KHUT01013049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           83 g           50 t
ORIGIN      
        1 gacgaaccac ccaaacgtta ttcggaatta ctgggcttaa agcgtgcgta ggcggtccgg
       61 aaagttaggt gtgaaatgcc acagctcaac tgtggaactg cgcttaaaac ttccagactt
      121 gagggatata gaggtgagca gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaataccgga ggcgaaggcg gctcactggg tatcttctga cgctgaggta cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013050
VERSION     KHUT01013050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgtgcgta ggcggcttgg
       61 taagtcggat gtgaaagccc tgggctcaac ccaggaagtg cattcgaaac tgccaagctt
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcagt ggcgaaggcg accacctgga cgaagattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013051
VERSION     KHUT01013051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggaatga ctgggcgtaa agggcgcgta gacggcgcat
       61 taagtctgct gttaaattct tcagcctaac tgaaggtcag cggtggaaac tgatgtgctt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013052
VERSION     KHUT01013052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggttccttgg
       61 caagtctggt gtaaaatggc gatgctcaac atcgtcatcg tgccggaaac taccgaggta
      121 gagtcactca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgccaaa agcgaaggca ggatgctggg ggtgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01013053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013053
VERSION     KHUT01013053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacggaggtt gcgagcgttg ttcggaatta ctgggcgtaa agggagcgca ggcggccgga
       61 taagtctaaa gtgaaatccc ggggctcaac ctcggaattg ctttggatac tgctcggctt
      121 gagtacgtga gaggtaggcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcggt ggcgaaagcg gcctactggc acgaaactga cgctgaggct cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013054
VERSION     KHUT01013054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtctggg gtgaaatcct tcggctcaac cgaagaactg ccttagatac tggtggtctc
      121 gagggaggta ggggtctgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcggactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013055
VERSION     KHUT01013055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           56 c           77 g           54 t
ORIGIN      
        1 tcccagcagt ccaagtcgca accacatttg ttgggtctaa aacatccgta gctcgcttcg
       61 taagtctcct gtgaaatcct tcatctcaag tgaagaagtg catgagatac tgcggagcta
      121 gagaccggga ggcgtatcga gtacgaaggg agtagcggta aaatgcgttg atcctctttg
      181 gactaccagt cgcgaaggcg cgatacgagc acggttctga cagtgaggga tgaaggctag
      241 gggcgcaaag tgg
//
LOCUS       KHUT01013056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013056
VERSION     KHUT01013056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c          101 g           34 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggcgggtaca
       61 tacgtcggcg gtgaaagttc ccggctcaac cgggagagga ccgccgaaac ggcgtacctg
      121 gaggacagga gaggcaggcg gaatgcccga aggagcggtg acatgcgttg agatcgggcg
      181 gaactccagt ggcgaaggcg gcctgctgga ctgctcctga cgctgaggcg cgaaagccag
      241 gggagcgaac gga
//
LOCUS       KHUT01013057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013057
VERSION     KHUT01013057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           90 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggtaac
       61 caagtcagat gtgaaattcc ggggcttaac tccggagctg catctgaaac tggttatctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013058
VERSION     KHUT01013058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           97 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcgggcggc
       61 acagtcagag gtgaaatctc acggcccaac cgtgaaacgg cctttgatac tggcggtctc
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcatgctggg ccttctctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013059
VERSION     KHUT01013059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           81 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggtggtaagg
       61 aaagtcagat gtgaaatccc ggggctcaac ctcggaactg catttgaaac ttcctttcta
      121 gaggataggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttagacctga cactgatgcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013060
VERSION     KHUT01013060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           91 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggagtca ctgggcttaa agcgtacgta ggcggatatg
       61 caggcgtcgt gtgaaatccc tcggctcaac cggggaactg cacggcgaac cgcatatctt
      121 gagaaaggta ggggctgtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaagaca ggcagctggg ccttttctga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013061
VERSION     KHUT01013061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           45 c           82 g           51 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatca ttgggcgtaa agggtgtgta gacggctaat
       61 taagtcaact gttaaattct tcggcctaac cggagatcag cagcagaaac tgataagcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccactcctga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013062
VERSION     KHUT01013062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ttgggcgtaa agcgcacgca ggtggcttct
       61 taagtctgac gtgaaagctc ctggcttaac cgggagaggt cgttgggtac tgggaggctt
      121 gaggcaagga gaggggcgta gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccaga ggcgcaagcg gcgccctggc cttggcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013063
VERSION     KHUT01013063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          101 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggccgca
       61 taagtcactt gtgaaatccc ggggctcaac tccggggctg caggtgagac tgtgtggcta
      121 gagcttggta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag atatcgggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccaatgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013064
VERSION     KHUT01013064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggatgct
       61 taagtcagat gtgaaagccc tgggcttaac ctaggaactg catctgaaac tgggcatcta
      121 gagtatggta gaggatggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gccgtctggg ccaatactga cgctgaggca cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013065
VERSION     KHUT01013065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           91 g           43 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggctgcccgc
       61 taagtcaggc gtgaaatccc ccggctcaac cggggaattg cacctgatac tggcgggcta
      121 gaggttggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgaaactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013066
VERSION     KHUT01013066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcaatg
       61 taagtcgggt gtgaaatccc ttggcttaac caaggaagtg cactcgatac tgtgttgctt
      121 gagtatcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgattactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013067
VERSION     KHUT01013067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagttagat gtgaaatccc taggcttaac ctgggaatgg catttaagac tgaatggcta
      121 gagtagaata gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcctgga ttcatactga cgctgaggcg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013068
VERSION     KHUT01013068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           87 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtaggg
       61 taagttcgat gtgaaagctc cgagctcaac tcggaaatgg cattgaaaac taccctgctc
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013069
VERSION     KHUT01013069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           98 g           49 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggatgg
       61 caagtcggtc gtgaaatcct tcggctcaac cgaagaacgt cgatcgatac tgccgttctt
      121 gaggattgca gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacactggt ggcgaaggcg ggtctttggg caatttttga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013070
VERSION     KHUT01013070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          101 g           43 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggcatgt
       61 caagtacgga gtgaaatctc actgctcaac ggtgagggtg ctttgtagac tggcaagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgaacctga cgctgaggcg cgaaggctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013071
VERSION     KHUT01013071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           81 g           56 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggatatg
       61 taagtaagaa gtgaaatccc tgggcttaac ccaggaactg ctttttagac tgcatgtctt
      121 gaattcggta gaggttagtg gaatatccag tgtagaggtg aaattcgcag atattggatg
      181 gaacaccagt ggcgaaggcg actaactgga ccgacattga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013072
VERSION     KHUT01013072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           81 g           57 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgcgta gacggtatgt
       61 taagtctatt gttaaatctt ctggcctaac cagaaatctg cagtaggtac tgatgtgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccactcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013073
VERSION     KHUT01013073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtttgg
       61 taagttggga gtgaaatccc ggggcttaac tccggaattg ctttcaaaac tgccaagcta
      121 gagtgtgata ggggacagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaagcg actgtctgga tcactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013074
VERSION     KHUT01013074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           85 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccgca
       61 taagtctgat gtgaaatctc gaagcttaac ttcgaaactg cattggatac tatacggctg
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013075
VERSION     KHUT01013075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c          101 g           41 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgtcg
       61 caagtcaacg gtgaaagctc gacgcttaac gtcgagtcgg ccgtcgagac tgcgaggctc
      121 gagcactgta gaggctcatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013076
VERSION     KHUT01013076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           95 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgt
       61 ccagtcgggg gtgaaatctt tcggctcaac cggaaaagtg cccccgatac tgacaggctc
      121 gagcgaggta ggggcagggg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ccctgctgga cctcacctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013077
VERSION     KHUT01013077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           92 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggactga
       61 caagtcgagg gtgaaatccc ggggctcaac ctcggaactg ccttcgatac tgtcagtctt
      121 gaggtcggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgattctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013078
VERSION     KHUT01013078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           96 g           40 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatga ttgggcgtaa agcgcgtgta ggcggcccac
       61 caagccggct gtgaaagccc acggcttaac cgtggaagtg cagccgggac tgatgggcta
      121 gagtcccgaa tagggtggcg gaatgcccag tgtagaggtg aaattcgtag atattgggta
      181 gaacatcggt ggcgaaggcg gccacctggg aggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013079
VERSION     KHUT01013079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           87 g           53 t
ORIGIN      
        1 tacgaagggt gttagcgttg ctcggaatta ctgggcgtaa agagcacgta ggcgggtttg
       61 taagtcgatt gtgaaagccc cgggcttaac ctgggaactg cagtcgaaac tgcaagtctt
      121 gaatgttcga gaggatggta gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaataccgga ggcgaaagcg gccatctggc gaaacattga cgctgaggtg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01013080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013080
VERSION     KHUT01013080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c           99 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccgc
       61 gtagtcggtg gtgaaatctt tcggctcaac cggaaaatcg cctccgatac tggcggcctc
      121 gaggggggta ggggcatgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013081
VERSION     KHUT01013081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           88 g           46 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 caagttcgat gtgaaagctc caggcttaac ctggaaatgg cattgaagac tgccccgctc
      121 gagggtcgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013082
VERSION     KHUT01013082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           96 g           47 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggagtta ctgggcgtaa agcgtgtgca ggcggcttgt
       61 tgcgtctacg gtgaaatctc ccggcttaac tgggagggtg gcgtggatac gggcaagctt
      121 gagggctgga gagggaagta gaactcccgg tggaacagtg aaatgtgtag agatcgggaa
      181 gaataccaga ggtgaagacg gcttcctggc cagtgcctga cgctcagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013083
VERSION     KHUT01013083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          106 g           35 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agggtgcgca ggcggcctac
       61 cgcgtggggg gtgaaatcgc tcggctcaac cgggcgggtg ctttccagac gggtaggctt
      121 gaggggcgga gaggggcgcg gaattccggg tggagcggtg aaatgcgtag agatccggaa
      181 gaacaccgac ggcgaaggca gcgccctgga cgccatctga cgctcatgca cgaaagcgcg
      241 gggagcgaac agg
//
LOCUS       KHUT01013084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013084
VERSION     KHUT01013084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           79 g           55 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggatgg
       61 tcagtcagat gtgaaatcct gaggctcaac ctcagaacag catttgaaac ttccattcta
      121 gagggtaggc ggagaaaaca gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaatacctgt ggcgaaggcg gttttctagg ttattcctga cgctaaagcg cgaaagtaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013085
VERSION     KHUT01013085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          109 g           35 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtga ctgggcgtaa agcgcgcgca ggcggcccgc
       61 gaggtgtcct gtgaaagccc cgtgctcaac gcgggagggt cagggcaaac cggcgggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtactga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013086
VERSION     KHUT01013086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c           90 g           44 t
ORIGIN      
        1 taccagctcc tcgagtggtc ggggcgatta ttgggcctaa agcatccgta gccggttccg
       61 taggtcctct gttaaatcca accgcttaac ggttgacctg cagaggatac cgcgggacta
      121 ggaggcggga gaggcggacg gtactccgcg tgtaggggta aaatcctttg atccgcggaa
      181 gaccaccagt ggcgaaggcg gtccgccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013087
VERSION     KHUT01013087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           95 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctgat
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cctttgatac tggtcagctt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctggc cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013088
VERSION     KHUT01013088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcgggtctt
       61 taagtgaggg gtgaaaggtt cgggctcaac ccggacactg cctttcatac tggagacctt
      121 gagtacagga gaggcgagtg gaatggtcgg tgtagcggtg aaatgcgtag atatcgatcg
      181 gaacacccat ggcgaaggca gctcgctggt ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013089
VERSION     KHUT01013089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           50 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggtggcttgt
       61 taagtcaggg gtgaaagttt tcggcccaac cggaaaatgg cctttgatac tggcgagctc
      121 gagtgcgtga ggggtaaccg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccaac ggcgaaggca ggttactggt acgcaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013090
VERSION     KHUT01013090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           59 c          107 g           39 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggctcgg
       61 caagcatggc gtgaaagccc ccggcctaac cggggagagt cgtcgtggac tgtcgagctt
      121 gagggcggta ggggttggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccaactggg ccgaccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013091
VERSION     KHUT01013091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           92 g           56 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggccttg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgctaaagac tgcagggcta
      121 gagtacggga tgggagagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaatacctgt ggtgtagacg gctctctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013092
VERSION     KHUT01013092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           56 c           99 g           45 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggctcgt
       61 taagtcggtt gtgaaaaccc ggggctcaac cccgggcctg catccgatac tggcgggctg
      121 gaggcagata ggggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga tctgtcctga cgctcaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013093
VERSION     KHUT01013093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           85 g           49 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggcggatttt
       61 caagtcagat gtgaaatctc ggagcttaac tccgaaactg cgtctgaaac tagaaatctt
      121 gagtgtcgga gagggatggg gaatttcctg tgtaggggta aaatccgtag agataggaag
      181 gaacatcaga ggcgaaggcg ccatcctaga cgaccactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013094             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013094
VERSION     KHUT01013094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggcggctaag
       61 taagtcagat gtgaaagccc ggggctcaac cccggaactg catttgaaac tgcatagctt
      121 gaatatcaga gagggtagcg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacatcgga ggcgaaggcg gctacctggc tgaatattga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013095
VERSION     KHUT01013095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           99 g           43 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtctgt
       61 taagtctgcg gtgaaatccc ggggctcaac tccggatatg ccgcggatac tggcggacta
      121 gagaccggta gaggcgagtg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccggttctga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013096
VERSION     KHUT01013096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           92 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggtcgaa
       61 tcagtcaggt gtgaaagccc ccggctcaac cggggaatgg cacttgatac tgtttgactg
      121 gaggacggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtatctga cgctaaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013097
VERSION     KHUT01013097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           98 g           43 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctggt gtgaaatctc cgagctcaac tcggaacccg catcagtgac tgccgtgctc
      121 gagtgtggga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccacaactga cgctgaggcg cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01013098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013098
VERSION     KHUT01013098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           90 g           50 t
ORIGIN      
        1 tacgtagggt ccgagcgtta tccggtatta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagacagtg gttaaagccc cgagctcaac tcgggatctg ccgttgtgac tgccaaactt
      121 gacggtagca ggggcaagcg gaattgtcgg tgtaggagtg aaatccgttg agatcgacaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctatcagtga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013099
VERSION     KHUT01013099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           47 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcacgta ggcggcccgg
       61 caagtcggtt gtgaaatccc tcggctcaac cgaggaattg ctgctgatac tgtcgggctt
      121 gagtcccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg actacctgga cgggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013100
VERSION     KHUT01013100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           88 g           50 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ttgggcttaa agggtacgta ggcggcactg
       61 tcagtcgggt gtgaaagccc tcggctcaac cgaggaattg cgtccgatac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcatctga cgctgaggta cgaaagctag
      241 gggagcgaag ggg
//
LOCUS       KHUT01013101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013101
VERSION     KHUT01013101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           96 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggcttgg
       61 caagtcgagt gtgaaatccc ttggcttaac taaggaattg cgctcgaaac tacctcgctg
      121 gaggacggga gagggaagtg gaactctcgg tgtaggggtg aaatctgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gcttcctggc ccgtacctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01013102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013102
VERSION     KHUT01013102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          107 g           35 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctcgc
       61 aacgcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac gggcgggctt
      121 gagtccggta gaggccgatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtcggctggg ccggaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013103
VERSION     KHUT01013103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           95 g           49 t
ORIGIN      
        1 gacggagggc gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtcttc
       61 taagtccctt gtgaaattcc ggggctcaac cccggagatg catgggatac tggttgactg
      121 gagaacggta gaggctagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctagctggg ccgtttctga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013104
VERSION     KHUT01013104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c          101 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtacgg
       61 caagtgtggt gtgaaatccc tgggcttaac ctggggcggt caccacggac tgcggtgctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013105
VERSION     KHUT01013105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          102 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgagta ggtggcttgg
       61 taagtctgtg gtgaaagcgc ggggctcaac cccgcgtcgg ccgtggatac tgctaggctc
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccagg ggcgaaggcg gcctgctggg cagttgctga cactgaatcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013106
VERSION     KHUT01013106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           88 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgaag
       61 taagtcgggt gtgaaatctc ggggcttaac cccgaaactg cattcgatac tgctttgctc
      121 gaggactgga gaggagattg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013107
VERSION     KHUT01013107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggcaggta ggttgttgtg
       61 caagtccggt gtgaaatccc acggctcaac cgtggaacgg cattggaaac tgcatgactt
      121 gagtatcgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cgattactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013108
VERSION     KHUT01013108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           87 g           58 t
ORIGIN      
        1 tacgagtgcg tcgagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggcgttg
       61 ttagtctctc gttaaattct tcggctcaac cgggggcatg cgggggaaac ggcaatgctt
      121 gaggttggta ggggtttctg gaactctaag tgtagcggtg aaatgcgttg atatttaggg
      181 gaacaccaaa agcgaaggca ggaaactgga ccatacctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013109
VERSION     KHUT01013109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           93 g           51 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ctgggcttaa agcgggtgta ggcggccttc
       61 caagtacctt gtgaaatacc tcggctcaac cggggaatgg ctgggtatac tgttaggctt
      121 gaggctgata ggggtgcctg gaactctagg tggagcggtg aaatgcgttg atatctagag
      181 gaacgcctgt ggcgaaagcg gggcactggg tcagtcctga cgctgagacc cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01013110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013110
VERSION     KHUT01013110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggtggtccgc
       61 taagtcgaat gtgaaatacc ccggctcaac cggggaactg cattcgatac tggcgggctc
      121 gaggtcggga ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactggc ccgaatctga cactgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013111
VERSION     KHUT01013111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 gacagagggc tcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggcttac
       61 taagtcggtt gtgaaatccc ccggctcaac cggggaactg cattcgatac tggtaggctc
      121 gaggacggga ggggtaagtg gaactccagg tggaacggtg aaatgtgtag atatctggag
      181 gaacaccggt ggcgaaagcg gcttactggc ccgtaactga cgctaaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013112
VERSION     KHUT01013112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           97 g           47 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggcggtcctt
       61 tgagtccttt gtgaaatccc ggggctcaac tccggatgtg cataggatac tggaggactg
      121 gagactggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccagttctga cgctcagccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013113
VERSION     KHUT01013113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           84 g           54 t
ORIGIN      
        1 tacgtaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcag
       61 taagttgggt gtgaaatccc ccggctcaac cggggaatgg cgctcaaaac tactgtgctc
      121 gaggaataca gaggtaagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcttactggg tattttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013114
VERSION     KHUT01013114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 tacgtaggga gcaagcgtta cccggattta ctgggcgtaa agagcgcgta ggcggcaagt
       61 taagtcttct gtaaaagctc ccggcttaac tgggagaggt caggagatac tggctagctc
      121 gagggcagta gaggagggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg tccctctggg ctgttcctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac cgg
//
LOCUS       KHUT01013115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013115
VERSION     KHUT01013115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           94 g           45 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ttgggcgtaa agcgcacgta ggcggcctgg
       61 taagatggac gtgaaagctc ccggcttaac tgggagaggt cgtacaagac taccaagcta
      121 gaggctggaa gaggagcgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcgctctggt tcagcactga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013116
VERSION     KHUT01013116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           88 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggtcaag
       61 taagtcgaat gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac tacttagctg
      121 gaggacggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctggg ccgatcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013117
VERSION     KHUT01013117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           78 g           61 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggtgggttat
       61 taagtcagtg gtgaaagcct gcagcttaac tgcagaactg ccattgatac tgataatctt
      121 gagtgtagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctcgctaaa ctataactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013118
VERSION     KHUT01013118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           97 g           47 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggttcgta ggcggtccaa
       61 gcagtcggac gtgacaatgc acggctcatc tgtgcgcggt cgttcgatac ttttggactg
      121 gaatcggtgc gaggtcggcg gaatgcccgg tgtagtggtg gaatgcgtag agatcgggca
      181 gaacctcaaa ggcgcaggca gccggctagg gccagattga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013119
VERSION     KHUT01013119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           84 g           47 t
ORIGIN      
        1 tacagagacc tcgagcgttg tccggattta ttgggcgtaa agggtccgca ggtggtacgg
       61 aaagtcgcgt gtcaaatctc agggctcaac cctgaaactg cgcgtgatac ttccgaacta
      121 gaggccggga gaggcagatg gaattgccgg tgtagcggta aaatgcgtta atatcggcaa
      181 gaacaccaaa ggcgaaggca gtctgctgga acggtcctga cactcaggga cgaaagcgtg
      241 ggtagcgaat gag
//
LOCUS       KHUT01013120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013120
VERSION     KHUT01013120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           86 g           49 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcgaaatta ctgggcgtaa agggcacgta ggcggcaaag
       61 tgtgtctgat gtgaaagacc ttggcctaac caaggaagtg cattggaaac tactttgcta
      121 gagtatgaga gaggatggcg gaattcccag agtagaggtg aaattcgtag atactgggag
      181 gaacacctga ggcgaaggcg gccatctgga tcataactga cgctgaggtg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013121
VERSION     KHUT01013121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           83 g           48 t
ORIGIN      
        1 tacagagacc tcaaacgttg tccggattta ttgggcgtaa agggtccgca ggcggcttca
       61 cacgtcgatg gtcaaatcat cgggcttaac ccggtggccg ccgtcgatac ggtgaagcta
      121 gagcatggga gaggtaagcg gaattgccgg tgtagtagta aaatgcgtta atatcggcaa
      181 gaacaccaaa tgcgaaggca gcttactgga acattgctga cgctcaggga cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01013122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013122
VERSION     KHUT01013122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          101 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcccgc
       61 caagtcaggg gtgaaagccc ccagctcaac tggggaaggg cctttgatac tggcgggctc
      121 gaggggagta ggggcggacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gtccgctggg ctcttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013123
VERSION     KHUT01013123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta ggcggtttgg
       61 taagtttgtg gttaaatctg gccgctcaac ggtcaacatg ccataaatac tgctgaacta
      121 gagcatggca gaggtaactg gaattctgtg tgtaggggta aaatccgttg atacacagag
      181 gaacaccaaa agcgaaggca ggttactggg ccatttctga cgctgttgaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013124
VERSION     KHUT01013124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           84 g           55 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggaataa ctgggcgtaa agggtgcgta gacggtatgt
       61 taagcttgtt gttaaattcc ctggcctaac cagggtcctg cagcgagaac tggcattcta
      121 gaggatgaga gagaagagtg gaatttccgg agtagcggta aaatgcgtag atctcggaag
      181 gaacatcgat ggcgaaagca gctctttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013125
VERSION     KHUT01013125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           44 c           76 g           54 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgtgta gacggcaaaa
       61 caagtcagtt attaaatctc ttggcttaac caagaaactg taaccgaaac tgttttgctt
      121 gaggatggaa gagagaagcg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013126
VERSION     KHUT01013126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           91 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggctgct
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaaatag cctccgatac tgggcagctc
      121 gagggagata ggggcacgcg gaactaatgg tggagcggtg aaatgcgtag atatcattag
      181 gaacgccggt ggcgaaagcg gcgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013127             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013127
VERSION     KHUT01013127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggtttct
       61 taagtcaagt ggtgaaaact atgggctcaa cccatagcct gcctttgaaa ctgggagact
      121 tgaggacagg agaggggagt ggaattccca gtgtagcggt gaaatgcgta gatattggga
      181 ggaacaccag tggcgaaggc ggctctctgg cctgtaactg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013128
VERSION     KHUT01013128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttgt
       61 taagtcaggt gtgaaatccc tgggctcaac ctgggaactg catttgatac tggcagacta
      121 gagtgtggta gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gccacctgga ccaacactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013129
VERSION     KHUT01013129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           96 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtcgttc gtgaaaactc aacgcttaac gttgagcctg cggtcgatac gggcagactt
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013130
VERSION     KHUT01013130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacgaaggga gctagcgttg ctcggaatta ctgggcgtaa agcgtttgta ggcggctata
       61 caagccagtg gtgaaagccc agggctcaac cctggaattg ccattgggac tgtatagcta
      121 gaggatgtta gaggtaagtg gaattcctag tgtaggagtg aaatccgtag agattaggag
      181 gaatatcgat ggcgaaggca gcttactggg acatttctga cgctgagaaa cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013131
VERSION     KHUT01013131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           89 g           46 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtcttt
       61 taagtcagat gtgaaatcct acggctcaac cgtggaactg cacctgatac tgaaagactt
      121 gaggacggta gaggtcggtg gaataccctg tgtaccggtg aaatgggcag atatagggtg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagctag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01013132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013132
VERSION     KHUT01013132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           87 g           48 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcag
       61 caagtcagat gtgaaatccg gaggcttaac ctccgaactg catccgaaac tgctgtgctc
      121 gagatctgta gagggacttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgt ggcgaaggcg aagtcctggg cagattctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013133
VERSION     KHUT01013133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggacctg
       61 taagtcgatt gtgaaatccc tgggcttaac ccgggaattg cagtcgaaac tgcaagtctt
      121 gagttctcga gaggatggtg gaattcctgg agtagtagtg aaatacgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gccatctggc gagacactga cgctaaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013134
VERSION     KHUT01013134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggatcc
       61 caagtctgcg gtgaaatcct ccagcttaac tggagaagtg ccgtggatac tgggattctc
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013135
VERSION     KHUT01013135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           82 g           59 t
ORIGIN      
        1 tacgaaggcg tcaagcgtta tccggattta ttgggcgtaa agagtgcgca ggaggatttt
       61 taagtttggt gttaaatctc ggggctcaac ttcgaggctg cattgaatac tgagaatcta
      121 gagtgcggga gaggctgata gaactcacgg tgtaggggtg acatccgttg atatcgtggg
      181 gaataccaaa ggcgaaggca ttcagctggc ccgttactga ctctcatgca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01013136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013136
VERSION     KHUT01013136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttgga
       61 caagtgagat gtgaaatccc cgggcttaac ccgggaactg cattttatac tgtctggctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013137
VERSION     KHUT01013137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgtgta ggtggtttga
       61 caagtttgat gtgaaatccc tgggcttaac ctaggaatgg cgtcaaaaac tgtaagactg
      121 gagtgagata gagggttgtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg acaacctgga tcttaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013138
VERSION     KHUT01013138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           95 g           43 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agggcgcgca ggtggtaggg
       61 taagtgtggc gtgaaagctc ccggctaaac tgggagaggt cgaaacagac tgcccaacta
      121 gagagtgata gaagtacgcg gaattcgggg tgtagcggtg aaatgtgtag agatcccgag
      181 gaacaccagt ggtgaaaacg gcgtactggg tcatatctga cactgaggcg cgaaagcttg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013139
VERSION     KHUT01013139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttat
       61 caagtcaggg gtgaaatcct ccggctcaac cggagaatag cccctgatac tggtagtctt
      121 gagggaggta ggggcctgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgggctgga cctcatctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013140
VERSION     KHUT01013140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtggat
       61 taagtctgat gtgaaagctc gcagctcaac tgcgaaatgg catgggatac taatctgcta
      121 gaggcttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caagacctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013141
VERSION     KHUT01013141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           97 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcat
       61 caagtccggg gtgaaagcct ccagcttaac tggagaagtg ccttggatac tggtgggctc
      121 gagtgaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ggctgctggt cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013142
VERSION     KHUT01013142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           92 g           48 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggattta ctgggcgtaa agcgcatgta ggcggttgag
       61 taagtcagat gtgaaagctc ctggctcaac taggagaggc catgtgaaac tagtcaacta
      121 gagggtggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccaaccctga cgctgtgatg cgaaagctag
      241 gggagagaac ggg
//
LOCUS       KHUT01013143             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013143
VERSION     KHUT01013143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           86 g           51 t
ORIGIN      
        1 tacgagggga gctagcgtta tccggaatga ttgggcgtaa agcgtttgta ggtggcttat
       61 aaagtctact gtcaaatatc gaggcttaac ctcgaaccgg cagtggagta ctcataagct
      121 tgagtgtggt agaggtagag ggaattccat gtggagcggt gaaatgcgta gagatatgga
      181 agaacaccag cggcgaaggc gctctactgg gccaagactg acactgagaa acgaaagctg
      241 ggggagcgaa aagg
//
LOCUS       KHUT01013144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013144
VERSION     KHUT01013144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           98 g           47 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttttg
       61 caagtcagag gtgaaagccc tgggctcaac ctgggaggtg cctttgaaac tgtgggactt
      121 gagtacggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccatctgga ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013145
VERSION     KHUT01013145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcgggacag
       61 tatgtcgggt gtgaaagccc agggctcaac cctggaagtg cgcccgaaac tgctgttctt
      121 gaggtcggga gggggtcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gcgacctgga ccgaatctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013146
VERSION     KHUT01013146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 tacagggggt gcaagcgtta ctcggaatta ttgggcgtaa agagcgtgta ggcggtttgt
       61 taagtctgat gtgaaagccc tgggcttaat ccaggaagtg catcggatac tggcagacta
      121 gagtgcagga gaggtgagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gctcactgga ctgtaactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013147
VERSION     KHUT01013147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c          100 g           37 t
ORIGIN      
        1 gacaggggtc tcgagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggacccg
       61 aacgcgtccc gtgaaatccc ccggctcaac cggggaactg cggggcgaac ggtgggtctc
      121 gaggcaggta ggggtcgccg gaaccgtagg tggagcggtg aaatgcgtag atatctacgg
      181 gaacgccgat ggtgaagacg ggcgactggg cctgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013148
VERSION     KHUT01013148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           83 g           55 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggtcttg
       61 ttagtctttt gttaaagctc ccggcttaac cgggaaaatg caagagaaac ggcaagactg
      121 gagggtgcaa gaggcttata gaactcatgg tgtaggggtg aaatccgtta atatcatggg
      181 gaataccgaa ggcgaaggca gtaagctggt gcacatctga cgctgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013149
VERSION     KHUT01013149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           93 g           45 t
ORIGIN      
        1 tacagaggtg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggtggctagg
       61 caagtcctgg gtgaaatccc tcggctcaac cgaggaactg ccttggaaac tgcctggctc
      121 gaggccggga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cactgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013150             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013150
VERSION     KHUT01013150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggcgtgt
       61 taagtgtgat gcgaaatctg gtggctcaac catctagact gtatcgcata ctggcatgct
      121 tgaggatggc agaggtaagt ggaattgata gtgtagcagt gaaatgcgta gatattatca
      181 ggaacaccaa tggcgaaggc agcttactgg gccacacctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01013151             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013151
VERSION     KHUT01013151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           79 g           60 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggtttta
       61 taagtttttt gttcaagact tcagcctaac tgaagaaaag caaaaaatac tgtactggct
      121 agagtatgat agagagaagt ggaattttcg gagtagcggt taaatgcgta gatctcgaaa
      181 ggaacgccaa tggcgaaggc agcttcttgg gtcattactg acgctgaggc gcgaaggcat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013152             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013152
VERSION     KHUT01013152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           39 c           79 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggattta ttgggcgtaa aggatgcgta gatggtggaa
       61 taagttattg gttgaagtcg agctcaactt gatggaagct agtaatactg ttctactgga
      121 gggcagaaga ggttaacgga attccatgcg taggggtgaa atccaatgat ccatggagga
      181 ccaccaaaaa aggcgaaggc agttaactat tctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa acgg
//
LOCUS       KHUT01013153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013153
VERSION     KHUT01013153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagat gtgaaatctc agggcttaac cctgaaactg catttgaaac tggagggctt
      121 gaggatcgga gagggcagtg gaattcccag tgtagcggtg atatgcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg actgcctgga cgacacctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013154
VERSION     KHUT01013154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggttttc
       61 caagtcaggc gtgaaagccc cgggctcaac ccgggaactg cgcttgatac tgtgagactt
      121 gagaccggga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013155
VERSION     KHUT01013155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacggagggg gctagcgtta ttcggaatca ctgggcgtaa agcgcgtgta ggcggcctgt
       61 aaagtcagag gtgaaatccc ggagcttaac ttcggaattg cctttgaaac tcacaggcta
      121 gaattcggta gaggatagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actgtctggg ccgatattga cgctaagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013156
VERSION     KHUT01013156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 tacgtagggt ccgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgc
       61 taagacaggt gtgaaatccc cgggcttaac ctgggaactg cgcttgtaac tagcaggcta
      121 gagtgcggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcgacactga cgctcatgca caaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013157
VERSION     KHUT01013157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           96 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggggtt
       61 caagtcggtg gtgaaatctt tcggcccaac cggaaaatcg cctccgatac tggatctctt
      121 gaggggggta ggggcatgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013158
VERSION     KHUT01013158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           92 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgtaa
       61 taagttagct gtgaaatccc tgggcttaac ctaggaactg cagctgatac tgttgggcta
      121 gagtgcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013159
VERSION     KHUT01013159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtctgat gtgaaatctc cgggctcaac tcggagtctg catcagaaac tgccgtgctc
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013160
VERSION     KHUT01013160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggagtta ctgggcgtaa agggtgcgta ggtggccgcg
       61 caagtcagat gtgaaatccg ggggcccaac ccccgaactg catccgaaac tgcgtggctt
      121 gagtgctata gagggacttg gaattttcgg tgtagcagtg aaatgcgtag atatcgaaaa
      181 gaagaccagt ggcgaaggct gggtcctggg tagcaactga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013161
VERSION     KHUT01013161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          104 g           45 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggtggcctgc
       61 caagtctgat gtgaaatcgc ccggctcaac tgggcggggt cgttggaaac tggcaagctg
      121 gaggacggta gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccgactggg ccggtcctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013162             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013162
VERSION     KHUT01013162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttaat
       61 taagtctcat gtgaaatcta cgggctcagc ctgtcaaagt gcatgagaaa ctaattagct
      121 agagtgtcga agaggagagc ggaattcccg gtgtagaggt gaaattcgta gagatcggga
      181 ggaacaccag tggcgaaggc ggctctctgg tcgacaactg acgctgagac tcgaaagcat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013163
VERSION     KHUT01013163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcacgta ggcggccctg
       61 taagttacat ctgaaaggtg acagctcaac tgtcaatggt ggtgtaagac tgcagagctt
      121 gaggtgagca aaagaatgtg gaactccgag tgtagcggtg gaatgcgtag agattcggaa
      181 gaacacccat ggcgaaggca gcattctggg ctcaacctga cgctgaggag cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013164
VERSION     KHUT01013164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          104 g           39 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggccgtg
       61 caagtcgtgc gtgaaagcgc ccggctcaac cgggcgaggt cgtgcgaaac tgcggggctt
      121 gaggcaggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gcacgctggg cctggcctga cgctcagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013165
VERSION     KHUT01013165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           93 g           52 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggaatca ttgggcttaa agagcacgta ggcggtcctt
       61 caagtgtctt gtgaaatacc tcggcttaac cgaggaattg ctgggcatac tgtaggactt
      121 gaggcaggta tgggcgagtg gaactcttgg tggagcggtg gaatgcgtag atatcaagag
      181 gaacgccggt ggtgaagacg actcgctggg cctgtcctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013166
VERSION     KHUT01013166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           90 g           45 t
ORIGIN      
        1 gacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgctcgta ggtggatcaa
       61 caagtccgca agcaaagacc cgggctcaac tcgggaatcc gtgtggaaac tgtcgatcta
      121 gaggtagtta gaggatgatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg atcatctggg gctaacctga cactgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013167
VERSION     KHUT01013167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           89 g           55 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccgtctgg
       61 taagcgtgtt gtgaaatgta ccggctcaac cggtgatgtg cagcgcgaac tgtcggactt
      121 gagtgcgcgc aacgcaggcg gaatttgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gcttgcggga gcgcaactga cgctgaagct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01013168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013168
VERSION     KHUT01013168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           94 g           46 t
ORIGIN      
        1 tacgggggga gcaagcgtta ctcggaatta ttgggcgtaa agggcgcgta ggcggtccta
       61 taagtcttct gtgaaagccc agggcttaac cttggaagtg caggagaaac tgcaggaatt
      121 gagttccgga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga cggatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013169
VERSION     KHUT01013169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           92 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggcgcgta ggctgtgggg
       61 taagtctggt gtgaaatctc gaagctcaac ttcgagactg cactggatac tgccttactc
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag agatcgtgag
      181 gaagaccggt ggcgaaggcg gatctctgga cagttcctga cgctgaggcg cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013170
VERSION     KHUT01013170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           90 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggcttgt
       61 taagtcgatt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac tagctagcta
      121 gaggacggca gaggaaggtg gaatgccaag tgtagaggtg aaattcgtag atatttggcg
      181 gaataccggt ggcgaaggcg gccttctgga ccggtcctga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013171
VERSION     KHUT01013171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           53 c          106 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgcgta ggcggcaggc
       61 caagtctgtg gtgaaatcgc ccggctcaac tgggcggggt ccatggaaac tggtcagctg
      121 gaggacggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggtcctga cgctgatgcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013172
VERSION     KHUT01013172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggattgg
       61 caagtcagag gtgaaatctc acggctcaac cgtgaaactg cctttgatac tgctgatctt
      121 gagttaggga gaggttgatg gaattctagg tgtagcggtg aaatgcgtag atatctagaa
      181 gaacaccagt ggcgaaggcg gtcaactggc cctaaactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013173             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013173
VERSION     KHUT01013173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           84 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggacttt
       61 taagtaggga gtgaaaggtt ggagctcaac tctaacactg ctccttatac tgggagtctt
      121 gagtgccgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca gctttctgga cggatactga cgctcaggca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01013174             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013174
VERSION     KHUT01013174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggtact
       61 taagtcagag gtcaaatccc agggctcaac cctggaactg cctttgatac tgggtaccta
      121 gagttcggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13326 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013175
VERSION     KHUT01013175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13326"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           96 g           48 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgagg
       61 taagtcagac gtgaaatccc ccggcttaac cggggaattg cgtctgatac tcccttgctt
      121 gaggccagga gaggagagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ctggttctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013176
VERSION     KHUT01013176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           82 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcgaaatta ttgggcgtaa agcgcgagca ggcggcgatg
       61 taagtcttgt gtgaaatctt cgggctcaac ccggaaaagt catgagaaac tgcattgcta
      121 gagtagatga gaggagagcg gaattcccag tgtagtggtg aaatacgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctctctggc atctcactga cgctcattcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013177
VERSION     KHUT01013177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           89 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggtgcgca ggcgggactg
       61 caagttggat gtgaaatacc ggagcttaac tccggagctg catccaaaac tgtggttctt
      121 gagtggagta gaggtaagcg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcttactggg ctctaactga cgctgaggca cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013178
VERSION     KHUT01013178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           81 g           50 t
ORIGIN      
        1 gacagaaggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgtctttc
       61 cacgtctggc attaaatcac ggggctcaac tccgtaacta tgttggaaac gagaaagatc
      121 gagtcactca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atacacgatg
      181 gaacgccaaa agcgaaggca ggatgctagg ggtgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01013179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013179
VERSION     KHUT01013179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           80 g           64 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggagcgta ggtggactgg
       61 taagtcagtt gtgaaagttt gcggctcaac cgtaaaattg cagttgatac tgtcagtctt
      121 gagtacagta gaggtgggcg gaattcgtgg tgtagcggtg aaatgcttag atatcacgaa
      181 gaactccgat tgcgaaggca gctcactgga ctgcaactga cactgatgct cgaaagtgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01013180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013180
VERSION     KHUT01013180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggtttag
       61 taagtgagtt gtgaaatccc tgggcttaac ctaggaactg caacttatac tgttagactt
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013181
VERSION     KHUT01013181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctacg
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaaatag cctccgatac tgcgtggctt
      121 gagggagata ggggcatgcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacgccggt ggcgaaagcg gcgtgctgga tcttctctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013182             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013182
VERSION     KHUT01013182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           91 g           47 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggcttg
       61 taagttagat gtgaaatctc cacggctcaa ccgggaaacc gcatctaaaa ctgcaagcct
      121 tgaggtatgg agagggaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacccg tggcgaaggc ggcttcctgg ccatatcctg acgctgaggt gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013183             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013183
VERSION     KHUT01013183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           92 g           47 t
ORIGIN      
        1 tacggagggc gcaagcgttg ctcggaatca ctgggcgtaa agggagcgca ggcggtcgga
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaattg cattcgatac tgtttgactt
      121 gagtgcggta gaggaaagcg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacaccagc ggcgaaggcg gctttctgga ccgacactga cgctgaggct cgaaagcgtc
      241 gggtagcaaa cggg
//
LOCUS       KHUT01013184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013184
VERSION     KHUT01013184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c          103 g           36 t
ORIGIN      
        1 gacagagggc gcgagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcgggttcg
       61 caagtcggaa gtgaaattcc ggggctcaac cccggagctg ctcccgaaac tgcgagtcta
      121 gggaccggta ggggccggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacctgt ggcgaaggcg gccggctggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013185
VERSION     KHUT01013185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           96 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtacgc
       61 caagtctggg gtgaaatctt tcggctcaac cggaaaattg cctgagatac tggcgtgctc
      121 gagggaggta ggggcagcgg gaactgccgg tggagcggtg aaatgcgttg atatcggcag
      181 gaacgccggt ggcgaaagcg cgttgctgga cctcttctga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013186
VERSION     KHUT01013186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           94 g           50 t
ORIGIN      
        1 tacggggggg gcgagcgttg ttcggaatta ctgggcgtaa agggtttgta ggtggcttgt
       61 tcagtcagac gtgaaatccc tcagcttaac tggggaactg cgtctgatac tagcgagctt
      121 gagggcagga gaggaacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgttctgga ctgcatctga cactgagaaa cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013187
VERSION     KHUT01013187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           86 g           56 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctggt gtgaaatctt cgagcttaac tcggaaactg cattggatac tattcggcta
      121 gagagttgga ggggggattg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg aatccctgga caacttctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013188
VERSION     KHUT01013188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           80 g           56 t
ORIGIN      
        1 tacgagtgcc tcaagcgtta tccggaatta ttgggcgtaa agggtgcgca ggcggaccta
       61 ttagtctcat gttaaaactt cgggctcaac ccgaaaaatg catgggaaac ggtaggtctt
      121 gaggatgtga gaggtgtaca gaactctagg tgtaggggtg aaatccgttg atatctaggg
      181 gaataccgaa tgcgaaggca gtacactggc acattcctga cgctcatgca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013189
VERSION     KHUT01013189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 gacggagggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggcggttgcc
       61 taagttggat gtgaaatccc gtggctcaac catggaactg catccgatac tgggcgactt
      121 gaggaaggtt ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccttttctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013190
VERSION     KHUT01013190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           80 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggagcgta ggtggctttg
       61 taagtcagtg gtgaaagctc ggagcttaac tccggaactg ccattgatac tgcttagctt
      121 gaatcggttc gaggcggatg gaatgtgaca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccaat tgcgaaggca gttcgccaga cccgtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013191
VERSION     KHUT01013191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttgt
       61 ttagtctgat gtgaaagccc tgggctcaac ccaggaagtg cattggaaac tggcaaactt
      121 gagtacggga gaggaaagtg gaatttcggg tgtaggggtg aaatccgtag atattcgaag
      181 gaacaccagt ggcgaaggcg gctttctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013192
VERSION     KHUT01013192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           92 g           45 t
ORIGIN      
        1 tacggggggt gcaagcgttg ctcggaatca ctgggcgtaa agcgtccgca ggcggtttga
       61 taagtcgggt gtgaaagccc ggggctcaac cccggaactg cattcgaaac tgtctgactt
      121 gagtacggga gagggtcgcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcgacctggc ccgatactga cgctcatgga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013193             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13344 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013193
VERSION     KHUT01013193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13344"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           94 g           49 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggttctg
       61 ttagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgagac tgcaggacta
      121 gagttcggga gagggtagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gctacctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013194
VERSION     KHUT01013194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           85 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtccgca gtcggttgtc
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaattg cattcgatac tggatagcta
      121 gagttcggca gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaagcg acttcctgga ccagaactga cgatcaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013195
VERSION     KHUT01013195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           79 g           56 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggagcgca ggcgggtcta
       61 taagtcgacg gtgaaatctt gcagctcaac tgcaaaactg ccgccgatac tgtagatctt
      121 gaatatgtca gagggaaacg gaattcatgg tgtagcggtg aaatgcgtag atatcatgaa
      181 gaacaccagt cgcgaaggcg gtttcctggg acattattga cgctcatgct cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013196
VERSION     KHUT01013196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgggca ggtggtcctt
       61 taagtccttt gtgaaatccc ggggctcaac tccggatgtg cataggatac tggaggactt
      121 gggactggta gaggcgagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ccagatccga cactcatccg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013197
VERSION     KHUT01013197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           85 g           58 t
ORIGIN      
        1 tacgaaggtt gcaagcgttg ttcggaatca ctgggcttaa agcgtatgta ggcggataag
       61 taagcgtttt gtgaaatccc tcggcttaac cggggaattg cttggcgaac tgcttatctt
      121 gaggcaaata ggggtacatg gaactcttgg tggagcggtg gaatgcgtag atatcaagag
      181 gaacgccagc ggcgaaagcg gtgtactgga tttgtcctga cgctcagata cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013198
VERSION     KHUT01013198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           88 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgtgg
       61 aatgtcagat gtgaaatccg ggggctcaac ctccgaattg catccgaaac ttccatgctc
      121 gagagctgta gaggggtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgt ggcgaaggcg agactctggg cagcttctga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013199
VERSION     KHUT01013199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           88 g           57 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcgggttat
       61 caagtcagat gtgaaattcc atggctcaac tgtggcgctg catctgaaac tggtaatctt
      121 gagtgatgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cattaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013200
VERSION     KHUT01013200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           52 c           77 g           49 t
ORIGIN      
        1 tacgagaaga acaagcgtta tccggaatca ttgggcgtaa agcgtgcgca agcggtttga
       61 taagtggggt gttaaatccc aacgctcaac gttggaatcg cgctccaaac tatcagacta
      121 gaggcaagta gaggctaacg gaattcccgg tgtagtagtg aaatgcgttg atatcgggag
      181 gaacaccaaa agcgaaagca gttagctagg cttgtcctga cgccgttgca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013201
VERSION     KHUT01013201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaggg
       61 caagtcggat gtgaaatccc gtggctcaac cacggaagtg cattcgaaac tgctcagcta
      121 gagttctgga gaggaaggcg gaatgcttgg tgtagaggtg aaattcgtag atatcaagca
      181 gaacacctgt ggcgaaggcg gccttctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013202
VERSION     KHUT01013202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           93 g           47 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggtcatg
       61 taagtcggat gtgaaagccc agggcttaac cctggaagtg catccgatac tgcatgactt
      121 gagtcccgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013203
VERSION     KHUT01013203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcggatctg
       61 taagtgcttt gtgaaatccc atggctcaac catggaattg ctcggcagac tgcagatctt
      121 gaggtcggta ggggctgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgaa ggtgaagaca ggcagctggg ccgattctga cgctgaggca cgaaagcgcg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013204
VERSION     KHUT01013204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           93 g           50 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggttggc
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaaaac tggccgactc
      121 gagtgtggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gcttactgga ccactactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013205
VERSION     KHUT01013205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          100 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcttgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccgtgcggac tggcaagctg
      121 gagcagagta gaggcgagtg gaatttcggg tgtagcggtg gaatgcgtag atatccgaaa
      181 gaacaccggt ggcgaaggcg gctcgctggg ctcttgctga cactgcagcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013206
VERSION     KHUT01013206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtcttc
       61 tttgtctggg gtgaaatcct tgagcttaac tcaagaaggg ccccagaaac ggggagacta
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtgactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013207
VERSION     KHUT01013207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           89 g           47 t
ORIGIN      
        1 tacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatgcc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaaatg cttggtatac tgggtgtctt
      121 gaggtgctca ggggcgagcg gaacagacgg tggagcggtg aaatgcgtag atatcgtctg
      181 gaacgccaat ggtgaagaca gctcgctggg agcattctga cgctgagacg cgaaagccag
      241 gggatcaaac ggg
//
LOCUS       KHUT01013208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013208
VERSION     KHUT01013208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           85 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggatacc
       61 taagtcgatt gtgaaagccc cgggctcaac ctgggaactg cattcgatac tgagtatcta
      121 gagtatggta gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccaatactga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013209
VERSION     KHUT01013209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           82 g           57 t
ORIGIN      
        1 tacgaaaggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtttga
       61 taagttggaa gtgaaatccc gaggcttaac ttcggaattg ctttcaaaac tgtcaagcta
      121 gaatatggta ggggacagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaagcg actgtctgga ccattattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013210
VERSION     KHUT01013210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 gacggaggga gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcctgt
       61 taagtcggct gtgaaatcgc tgggctcaac tcagcaaccg cagtcgatac tggcaggctc
      121 gagttcggga gagggaagcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctttctgga ccgatactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013211
VERSION     KHUT01013211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggattta ctgggcgtaa agcgcgtgta ggcggttggt
       61 taagttggat gtgaaagctc ctggctcaac taggagaggt cgttcaaaac tggctaacta
      121 gaggacagta gagggaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ctgtgcctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013212
VERSION     KHUT01013212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           56 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggcatga
       61 aaagtctggt gttaaatcct ggagctcaac tccagttcgg cattggatac tttcaagcta
      121 gagtgcagaa gaggcaaggg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgtaagcg ccttgctggt ctgttactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01013213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013213
VERSION     KHUT01013213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttctg
       61 taagtcagat gtgaaaaccc ggggcttaac tccggaaggt cgtttgatac tgcagagcta
      121 gagttttgaa ggggacagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccaga ggcgaaggcg actgtctggt caaaaactga cactgaggca cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013214
VERSION     KHUT01013214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 tcagtcggat gtgaaatccc tgggctcaac ctaggaattg cattcgatac tgcctggcta
      121 gagtgtggta gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaggcg acttcctgga ccaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013215
VERSION     KHUT01013215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttgg
       61 taagtcggat gtgaaagccc cgagcttaac tcgggaattg cattcgatac tgcctgactg
      121 gagtgtggta gagggtagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccaacactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013216
VERSION     KHUT01013216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcgagcattg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggtctt
       61 taagtcgatt gtgaaagccc agggcttaac cctggaacag cagtcgatac tattgatctt
      121 gaatacctga gagggtgtcg gaattcccgg tgtaggagtg aaatccgtag atatcgggag
      181 gaacatccga ggcgaaggcg gacacctggt ggtgtattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013217
VERSION     KHUT01013217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           47 c           83 g           50 t
ORIGIN      
        1 tacgaaggtg acaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcgggtttg
       61 aaagtcgatt gtgaaaaccc tgggctcaac ccgggacctg cagtcgaaac ttcaagtctt
      121 gaatgctcga gaggatagtg gaattccagg agtagaagtg aaattcgtag atatctggag
      181 gaacaccgga ggcgaaagcg gctatctggc gagacattga cgctaaggtg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013218
VERSION     KHUT01013218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           81 g           56 t
ORIGIN      
        1 gacgtaggtt gcgagcgtta tccggaatta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagttggat gtcaaattcc aaagctcaac tttgggtcgg tatccaaaac tagcaagcta
      121 gagacagaga gaggtagatg gaattctgca tggaggggta atatccgtag atatgcagag
      181 gaacaccaaa agcgaaagca gtctactggc tctgttctga cgctgaggaa cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013219
VERSION     KHUT01013219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtgaag
       61 taagttgggt gtgaaatgcc gcagctcaac tgcggccagc actcgatact gcttcgctag
      121 agcactagag agggggctgg aatttacggt gtagcagtga aatgcgtaga tatcgtaagg
      181 aacgccagtt gcgaaagcga gcccctggat agtatgctga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013220
VERSION     KHUT01013220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           63 c          109 g           34 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggcgcgca ggcggtgctg
       61 tgcgtcggcg ctgaaagcgc ccggcttaac cgggtgaggt gcgccgatac gacggcactg
      121 gaggcaggga gaggctggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgcccgt ggcgcaagcg gccagctggt cctgacctga cgctgaggcg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013221
VERSION     KHUT01013221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 gacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgtgg
       61 caagtcgaaa gtgaaatctc caggcttaac ttggaggctg cttccgagac tgccatgcta
      121 gagtgcggga gaggcgagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaggcg gctcgctgga ccgcaactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013222
VERSION     KHUT01013222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 aacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggtggttttc
       61 taagtttcgt gtgaaagccc acggctcaac cgtggaacgg cacggaaaac tggaagactt
      121 gaatacggga gaagagggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctctctaga ccgatattga cgctcatacg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013223
VERSION     KHUT01013223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           96 g           44 t
ORIGIN      
        1 aacgtgggag acgagcgtta tccggaatta ctgggcgtaa agcgcatgca ggcggtcctt
       61 taagtcggat gtgaaagcgc ctggctcaac taggcgagga cattcgagac tggaggagta
      121 gagggtggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccaaccctga cgctcagatg cgaaagctag
      241 gggagagaac ggg
//
LOCUS       KHUT01013224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013224
VERSION     KHUT01013224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           90 g           44 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggctgttgac
       61 taagtcaggt gtgaaagccc ccggctcaac cggggaacag cacctgatac tggtcgactc
      121 gagaatggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccatttctga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013225
VERSION     KHUT01013225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           93 g           47 t
ORIGIN      
        1 gacgtaggtg gcgagcgtta ctcggattca ctaggcgtaa agcgcgtgta ggcgggtgcc
       61 taagtccgcc gtgaaattta cgggctcaac ccgtgaaggt cgacggatac tgggcgtctt
      121 gagtgcggta gagggtactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ggtacctggg ccgttactga cgctaagacg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013226
VERSION     KHUT01013226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           78 g           59 t
ORIGIN      
        1 tacgtagggt acaagcatta atcggattta ctgggcgtaa agggtgcgta ggcggatcga
       61 caagtcaggt gtgaaattcc ggtactcaat accggagctg cacttgaaac tatctttcta
      121 gaggtcagac ggaggaaacg gaattccatg tgtagcggtg aaatgcgtag atatatggaa
      181 gaacacctgt ggcgaaagcg gtttcctagt ttgattctga cgctgatgca cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013227
VERSION     KHUT01013227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggcttgg
       61 taagtttctt gtgaaagctc ccggcttaac tgggagaggt caaggaatac tgccaggcta
      121 gagtgtggta gaggaagcta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013228
VERSION     KHUT01013228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           92 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggctagg
       61 taagtttagt gtgaaatctc ccggctcaac tgggagggtg cgccgaatac tgcctggctg
      121 gagtacggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013229
VERSION     KHUT01013229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           93 g           50 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcaa
       61 caagtcgggg gtgaaatcct gcagcttaac tgcagaagtg ccttcgatac tgttggtctg
      121 gagggagata ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013230
VERSION     KHUT01013230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta gatggcttcg
       61 taagtcggat gtgaaatccc cgggcttaac ctgggaactg cattcgaaac tgcgttgctc
      121 gaatgtggaa gagggaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcagt ggcgaaagcg gcttcctggt ccaacattga cattcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013231
VERSION     KHUT01013231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           94 g           48 t
ORIGIN      
        1 tacagagggc ccgagcgttg cgcggaatca ctgggcttaa agggtgcgta ggcggaggcg
       61 taagtgtctt gtgaaagccc acggctcaac cgtggaattg ttgggcatac tgcgtctctt
      121 gaggctggta ggcgccgccg gaactgtagg tgtagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg ggcggctagg ccagttctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013232
VERSION     KHUT01013232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           86 g           55 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaataa ttgggcgtaa agagtgcgta ggcggtgcgt
       61 taagtctatt gttaaatact ctggcctaac tggagaaatg caatggaaac tggcgtgcta
      121 gagggtggga gagagaagtg gaattctcgg agtagcggta aaatgcttag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013233
VERSION     KHUT01013233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           92 g           43 t
ORIGIN      
        1 cacggaggcc ccgagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggcgagg
       61 caagtcagat gtgaaagccc ggggctcaac cccggaattg catccgatac tgccttgctt
      121 gagggttgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtgg atatcgtgag
      181 gaagaccaat ggcgaaggca ggtctctgga caactcctga cgctgaggca cgaaggccag
      241 gggagcgaaa agg
//
LOCUS       KHUT01013234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013234
VERSION     KHUT01013234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggctgcgtgg
       61 taagtcgagt gtgaaattta ggggcttaac ccctaaactg catccgatac tgccatgcta
      121 gaggactgta gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctggg caggtcctga cgctcatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013235
VERSION     KHUT01013235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccttt
       61 taagtcagac gtgaaatatt tgggcttaac ccaaaggctg cgtttgaaac tggagggctt
      121 gaggaccgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actgcctgga cggcacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013236
VERSION     KHUT01013236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           83 g           54 t
ORIGIN      
        1 tacagagacc acaagcgtta tccggattta ttgggcgtaa agggtccgta ggcggcttgg
       61 taagtcattc gtcaaatctt cgggctcaac ccggaggctg cgggtgatac tgtcaggctt
      121 gagactggga gaggcatatg gaattgctgg tgtagcggta aaatgcgtta atatcagcaa
      181 gaacaccaaa ggcgaaggca atatgctgga acagttctga cgctcaggga cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013237
VERSION     KHUT01013237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggccatt
       61 taggtagggt gtgaaatccc gcagcttaac tgcggaacgg cgctctaaac cgggtggctc
      121 gagtgaggca ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tcttaactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013238
VERSION     KHUT01013238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtaagg
       61 taagttggat gtgaaatccc ggggcttaac cccggaactg cattcaatac tgccatacta
      121 gagtactgga gaggagattg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg aatctctgga cagttactga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013239
VERSION     KHUT01013239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           99 g           44 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgcgca ggcggctttg
       61 caagtcggac gtgaaagctc ctggcttaac tgggagaggc cgttcgaaac tgcgaggctt
      121 gaggttggga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013240
VERSION     KHUT01013240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           89 g           45 t
ORIGIN      
        1 taccagctcc tcgagtggtc gggacgatta ttgggcctaa agcatccgta gcctgttccg
       61 taggtcctcc gttaaatcca acggcttaac cgttgacatg cgggggatac cgcgggacta
      121 ggaggcggga gaggtgggcg gtactccacg tgtaggggta aaatcctttg atccgtggaa
      181 gaccaccagt ggcgaaggcg gcccaccaga acgcgctcga cggtgaggga tgaaagctgg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013241
VERSION     KHUT01013241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           89 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgtgta ggcggccttt
       61 taagtgagat gtgaaatcct gcagctcaac tgcagaactg catttcaaac tggaaggctt
      121 gagtgtggga gaggaaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gctttctggc ccaccactga cgctgagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013242
VERSION     KHUT01013242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcgtgca ggtggtttgg
       61 taagttggat gtgaaagctc ctggcttaac tgggagaggt cgttcaaaac taccagactt
      121 gagtgtggga aaggaaggcg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctggc ccacaactga cactcagatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013243
VERSION     KHUT01013243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           84 g           52 t
ORIGIN      
        1 gacgagggac cctagcgttg ttcggaatca ttgggcgtaa agcgggtgca ggtggcttta
       61 taagtcagat gtgaaagccc tgggcttaac ccaggaagtg catttgatac tgcaaagctt
      121 gagtgctgga gaggttactg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg ggtaactggc cagacactga cactcaaacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013244
VERSION     KHUT01013244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           45 c           72 g           58 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggattca ttgggcgtaa agcgtctgca ggtggatatt
       61 taagtctttt gttaaagctt tgggctcaac ccaaaatctg caaaagaaac tatgtatctt
      121 gagtctggta gaggtaaagg gaatttccgg tggagcggtg aaatgcgtag atatcggaaa
      181 gaacaccaac tgcgaaggca ctttactggg ccacgactga cactcagaga cgaaagctaa
      241 gggagcaaac agg
//
LOCUS       KHUT01013245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013245
VERSION     KHUT01013245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           97 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattaa ctgggcgtaa agggcgcgta ggcggtgagg
       61 tgtgagtgat gtgaaagccc cgggcttaac ctgggaagcg catcgctgac tacctgactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013246
VERSION     KHUT01013246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtttgg
       61 taagtccgct gtgaaagtcc agggctcaac cctggaaagc cggtggatac tgtcaaactc
      121 gagtgcggga gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctgga acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013247
VERSION     KHUT01013247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggtgcgta ggcggtaaga
       61 tgtgtgcgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac tgttttgcta
      121 gagtatagga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ctaatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013248
VERSION     KHUT01013248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           60 c           96 g           41 t
ORIGIN      
        1 gacgaaccgt gctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtgg
       61 acagtccgtg gtgaaatcct ccggctcaac cggagaactg ccgcggatac tgccactctc
      121 gaggggtgta ggggcatgcg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013249
VERSION     KHUT01013249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacggagagg gcaagcgttg ttcggaatta ctgggcataa agcgcacgta ggcgggtcgg
       61 aaagtcagtt gtgaaatccc ggagctcaac tccggaactg cttctgaaac ttccggtctg
      121 gagggtgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaagcg actctctggc tcatttctga cgctaaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013250
VERSION     KHUT01013250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           94 g           49 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatga ctgggcgtaa agggagcgca ggcggttcga
       61 taagtctgga gtgaaagccc ggggctcaac cccggcgggt ctttggatac tgtcgaactt
      121 gagtacggaa gaggagagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctctctggt ccgatactga cgctcatgct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013251
VERSION     KHUT01013251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           80 g           51 t
ORIGIN      
        1 tacagagacc ttaaacattg tccggattta ttgggcgtaa agggtccgca ggtggcttca
       61 tgcgtcgatg gtcaaacctt tgggctcaac ccaaagactg ccgtcgatac ggtgaagctt
      121 gagcatggga gaggtacgcg gaattgccgg tgtagtagta aaatgcgttc atatcggcaa
      181 gaacaccaaa tgcgaaggca gcgtactgga acattgctga cactcaggga cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01013252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013252
VERSION     KHUT01013252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcggaactg
       61 caagtcagat gtgaaattcc ggggctcaac cccggaactg catttgaaac tgtagtcctt
      121 gagtatcgga gaggcaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttgctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013253
VERSION     KHUT01013253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           89 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgagta ggcggttatt
       61 taggccagcc gtgaaagccc atggctcaac catggaagtg cagctggaac cggatagcta
      121 gagtatcgac gaggatggtg gaattcccag tgtagaggtg aaattcgtag agattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg agataactga cgctgagtcg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01013254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013254
VERSION     KHUT01013254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           83 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ctgggcgtaa agagcgcgta ggctgttcga
       61 taagtttgat gtgaaagccc tggactcaat tcaggaagtg catcgaatac tgtcggacta
      121 gagtatagga gaggacaacg gaatttctgg tgtagaggtg aaattcgtag atatcagaag
      181 gaacaacagt agcgaaggcg gttgtctgga ctattactga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013255
VERSION     KHUT01013255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 gacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcatgggca ggtggttcgg
       61 tcagtctgtt gtgaaagcct tgggcttaac ccagggaggt caatggatac tgccggactt
      121 gaatcgagga ggggtgactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaat ggcgaaggca ggtcactgga ctcgtattga cactcatcca tgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01013256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013256
VERSION     KHUT01013256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           46 c           92 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtttgt
       61 tgcgtcgact gtgaaatccc aaggctcaac cttgggtctg cagtcgatac gggcaaacta
      121 gagtatggta ggggagactg gaattcttgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccattactga cactgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013257
VERSION     KHUT01013257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           96 g           48 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggt
       61 taagtcaggg gtgaaatccg gcagcctaac tgtcgaatag cttttgatac tggccatctt
      121 gaggggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctggg ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013258             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013258
VERSION     KHUT01013258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           64 c          111 g           31 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccggattta ttgggcgtaa agggcgcgta ggcggccccc
       61 agcagtggcg cgtggaatct cccggctcac ccgggagggg ccgtgccaga cgggggggct
      121 ggaggcgggc agagggtcgc ggaacgggcg gagtaggggt gaaatccgta gagaccgtcc
      181 ggaacaccga tggcgaaggc agcgacctgg gcccgtcctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cgga
//
LOCUS       KHUT01013259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013259
VERSION     KHUT01013259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           81 g           57 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttttg
       61 ttagtcttct gttaaagctc ccggcttaac cgggaaagtg cagaggaaac ggcaagacta
      121 gaggatgtga gaggtttatg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca ataaactggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013260             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013260
VERSION     KHUT01013260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           46 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agggttcgca ggcgggaacc
       61 tcaagtcagg tgtgaaatcc ccgggctcaa cccgggacgt gcatctgaaa ctgggtttct
      121 tgagtactgg agagggcagt ggaattgctg gtgtaggagt gacatccgta gagatcagca
      181 ggaacaccgg aggcgaaggc gactgcctgg ccagatactg acgctgagga acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013261             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013261
VERSION     KHUT01013261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           97 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggttggg
       61 caagtcgggt gtgaaacctc caggctcaac ctggcgtcgc cacccgatac tgccctgact
      121 cgagtccggt aggggagtgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggcactctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013262             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013262
VERSION     KHUT01013262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           50 c          101 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa aaggcttgta ggtggccacg
       61 caaagtcgga tgtgaaatct cccggctcaa ctgggagggg tcattcgata ctggcgggct
      121 cgaggccggt agggggaagt gggattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc gacttcctgg accggttctg acactgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01013263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013263
VERSION     KHUT01013263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           96 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgaga
       61 taagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catagcaaac tgtctgactg
      121 gagtatagga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgccaat ggcgaaggca gccacctggc cagatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013264             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013264
VERSION     KHUT01013264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           52 c          102 g           49 t
ORIGIN      
        1 tacggaaggg gcgagcgttg ttcggaatca ttgggcgtaa aggggtgagt aggcggttcg
       61 gtgtgtctct cgtgaaagcc cacggctcaa ccgtggtgtg tcgggagaaa ctgccggact
      121 tgaggctggg aggggctggt ggaattccct gtgtagcggt gaaatgcgta gagatggggg
      181 agaacactcg tggcgaaggc ggccagctgg tccagttctg acgctcagtc ccgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013265
VERSION     KHUT01013265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           84 g           57 t
ORIGIN      
        1 gacgaggggt gcgagcgtta ttcggaatta ttgggcgtaa agggtgtgta ggcggcatat
       61 taagtcaact gttaaatttt ctggcctaac tggaaacatg cggtagaaac tgatgtgctt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013266
VERSION     KHUT01013266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           89 g           53 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctctc
       61 taagtcagaa gtgaaagccc caggcttaac ctgggaagtg cttttgatac tggagagctt
      121 gaatgtggga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ccaacattga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013267
VERSION     KHUT01013267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtccga
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg ccccagaaac ggtcggactc
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtgactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013268
VERSION     KHUT01013268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           93 g           51 t
ORIGIN      
        1 tacggggggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggtggtttgt
       61 taagtctgtt gtgaaagccc tgggctcaac ccaggaagtg cactggatac tggcagactt
      121 gaatacggga gaggagagcg gaattcctgg tgtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgacattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013269
VERSION     KHUT01013269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggtgtaa agggcgtgta ggcgggttag
       61 taagtcagat gtgaaattcc aaggctcaac cttggcgctg catctgaaac tactgatctt
      121 gagtgtcgga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013270
VERSION     KHUT01013270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           87 g           58 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtggattct
       61 taagtcggtc gtgaaatcct gtagcttaac tgcagaattg cgttcgatac tgggaatctt
      121 gagtgcggca gagggagatg gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaacaccggt agcgaaggcg gtctcctggt ccgcaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013271
VERSION     KHUT01013271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           90 g           48 t
ORIGIN      
        1 tacgaactgc acaaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggcctgg
       61 agggtgaggt gtgaaatccc acagctcaac tgtggaattg cgcttcaaac cgccaggctt
      121 gagggagaca ggggtgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatttg
      181 gaacaccggt ggcgaaggcg gctcactggg tctcttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013272
VERSION     KHUT01013272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           61 c          109 g           36 t
ORIGIN      
        1 cacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggtctgg
       61 tgcatctctg gtgaaagccc cgggctcaac ccgggagggt ccagggcgat ggccagactg
      121 gaggcagggc gagggcggtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gccgcctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013273
VERSION     KHUT01013273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgagta ggtggctcgt
       61 taagcgtgtg gtgaaagcct ggggctcaac cccaggtctg ccatgcgaac tggcaagctt
      121 gagcatagta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag atatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg ctattgctga cactgaatca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013274
VERSION     KHUT01013274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           89 g           54 t
ORIGIN      
        1 gacggggggt cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggttttg
       61 taagtcagga gtgaaagccc tgggctcaac ccaggaagtg cttttgatac tgcaaaactt
      121 gagtgtggga gaggatcgtg gaattcctag tgtagtggtg aaatacgtag atattaggag
      181 gaacaccggt ggcgaaggcg gcggtctggc ccaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013275
VERSION     KHUT01013275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggattta ctgggtgtaa agggcgcgta ggcgggactg
       61 caagtcagat gtgaaatgtg agggcttaac cctcaagctg catttgaaac tgtggttctt
      121 gagtatcgga gaggcaggcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcctgctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013276             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013276
VERSION     KHUT01013276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           50 c           77 g           53 t
ORIGIN      
        1 tacgagggcc ccaagcatta tccggattta ttgggcgtaa agcgtccgta ggtggtttat
       61 cgcatctcct gttaaatctc gaggctcaac ctcgagtcag cgggagagat ggacaaacta
      121 gagatcggaa gaggcaaacg gaattgctgg tgtaggggtt aaatccatta atatcagcaa
      181 gaacaccaaa tgcgaaggca gtttgctagg acgaatctga cactcagtgg acgaaagcgt
      241 ggggagcgaa tggg
//
LOCUS       KHUT01013277             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013277
VERSION     KHUT01013277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           49 c           84 g           49 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtttctttc
       61 cacgtctggt gtaaaagcac tggggctcaa ccccgtgtac gtaccggaaa cgagagagat
      121 agagtcattc agaggtatct ggaatgtcgt gtgtaggggt aaaatccgta gatccacgat
      181 ggaacgccaa aagcgaaggc aggatactgg ggatgtactg acactcagag acgaaagcgt
      241 ggggagcaaa gggg
//
LOCUS       KHUT01013278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013278
VERSION     KHUT01013278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           98 g           42 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggcctag
       61 caagtcagct gtgaaagccc caggctcaac ctgggaattg catctgaaac tgccgggcta
      121 gagttcagga gaggagggcg gaactcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ctgaaactga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013279
VERSION     KHUT01013279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggcggttcgg
       61 ttagtctgtt gtgaaagccc cgggctcaac ccggggaggc caatggatac tgccggactt
      121 gaatgtgaga gaggtgactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaat ggcgaaagca ggtcactgga tcactattga cgctcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01013280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013280
VERSION     KHUT01013280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           82 g           49 t
ORIGIN      
        1 tacgaaggcc ccaagcgtta tccggaatca ctgggcgtaa agggtccgta ggtggtttag
       61 ttagtctcat gttaaatctc caggctcaac ctggaaacag cgtgggaaac ggctaaacta
      121 gagagtgcga gaggtctgca gaacgcatgg tgtaggggtg aaatccgttg atatcatgcg
      181 gaataccgaa agcgaaggca gcagactggc gcatttctga cactgaggga cgaaagccag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013281             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013281
VERSION     KHUT01013281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           86 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggataac
       61 taagtcagat gtgaaaaccc taggcttaac caagggagtg catttgaaac tggttatcta
      121 gagtgcggga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg acttactgga ccgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013282
VERSION     KHUT01013282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c          100 g           39 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ctcggaatca ctgggcttaa agggcatgta ggcgggcgag
       61 taagcgcctt gtgaaatccc ccggctcaac cggggaaccg cttggcgaac tgctcgtctg
      121 gagggaggca ggggcgaccg gaaccctggg tggagcggtg aaatgcgtag atatccaggg
      181 gaacgccgga ggagaaatcg ggtcgctggg cctttcctga cgctgaggtg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013283
VERSION     KHUT01013283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggttttg
       61 taagtcagtg gtgaaagccc ggagcttaac ttcggaattg ccattgaaac tgcaaagctt
      121 gaatatagtt gaagtgggcg gaatacaaca tgtagcggtg aaatgcttag atatgttgta
      181 gaacaccaat tgcgtaggca gctcactaaa ctatcattga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01013284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013284
VERSION     KHUT01013284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           96 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtgaga
       61 tgtgagcgat gtgaaagccc cgggcttaac ctgggaagag catagtttac tatctgactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013285
VERSION     KHUT01013285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          104 g           39 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggaatta ctgggcgtaa agcgctcgta ggcggtcgcg
       61 tacggcggtg gtgaaagctc ccggctcaac tgggagaggc ccgccgcaac ggcgtgactc
      121 gagggtggta gaggcaaatg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gtttgctggg ccatgcctga cgctgaggag cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013286
VERSION     KHUT01013286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           91 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgggg
       61 tgagtccggt gtgaaatctc cgagcttaac tcggaaatgg catcggatac tattccgctg
      121 gagggtcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013287
VERSION     KHUT01013287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           87 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggtgggtctt
       61 taagtcgatt gtgaaatccc ggggcttaac cccggaattg cagtcgaaac tattgatctt
      121 gaatgcctga gaggatgtcg gaattcccgg tgtagaagtg aaattcgtag atatcgggag
      181 gaacacctga ggcgaaggcg gatgtctggc ggtgcattga cactgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013288
VERSION     KHUT01013288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggttcgg
       61 aaagtctctt gtgaaagctc ccggcttaac tgggagaggc caagggaaac ttccggactt
      121 gagtgtggta gaggatgctg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccaat ggcgaaagca ggcatctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013289
VERSION     KHUT01013289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c          101 g           45 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcgcgt
       61 cgagttaggg gtgaaaggcc tgggctcaac ctgggaactg cctttaatac tgacgtgctg
      121 gagtccggga gggggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccacctggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013290
VERSION     KHUT01013290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           88 g           54 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggtagta
       61 caagtctgaa gtgaaatgcg ggggctcaac ccctgaactg ctttggaaac tgtattacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013291
VERSION     KHUT01013291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           97 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggctgat
       61 caagttggac gtgaaagctc ccggcttaac tgggagaggt cgtacaaaac tgatcggctc
      121 gaggctgaga gaggagagca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctctctggc tcagaactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013292             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013292
VERSION     KHUT01013292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           98 g           47 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggtggcgcgg
       61 tgcgtctgtg gtgaaagtct tcggcccaac cggagaatag ccgcggatac ggccgtgctt
      121 gagtgcatta ggggtagggg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccagt gccgaagggg ccctactggg atgcaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013293
VERSION     KHUT01013293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           61 c          105 g           36 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggtcaga
       61 ggcgtcggtg ctcaaagtcc accgcttaac ggtgggaggc gtgccgatac gctctgactg
      121 gagggcgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg gccacctagc ccgcacctga cgctgaggcg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01013294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013294
VERSION     KHUT01013294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           98 g           39 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggcggatgac
       61 taagttactg gtgaaagcgc cgggctaaac tcggcgaggt ccggtaagac tggtgatcta
      121 gagggggtca gaggggtgcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcaccctggg accaacctga cgctgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013295
VERSION     KHUT01013295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggccaga
       61 taggtccgct gtgaaaactc gaggcttaac ctcgagaggt cggtggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013296
VERSION     KHUT01013296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           39 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggtgcgta ggtggtgaga
       61 taagtcagtt gtgaaaggcc tgggcttaac ctgggaattg caattgaaac tgtgtgacta
      121 gagttgagta gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctctctgga ctcaaactga cgctgaagca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013297
VERSION     KHUT01013297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggttcaa
       61 tcagtctgct gtaaaagtcc agtgctcaac gctggaaggt cggtggatac tgttgaacta
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013298
VERSION     KHUT01013298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           81 g           59 t
ORIGIN      
        1 tacgagtggt ccaagcgtta tccggaatta ttgggcgtaa aggatgtgta ggtggttgta
       61 ttagtcgggt gtcgaacctc tgggcttaac ccggagacag cattcgaaac ggtacgacta
      121 gagtatgtta ggggtaagcg gaactcatag tgtaggggtg aaatccgttg atattatggg
      181 gaacaccaaa agcgaaggca gcttactgga acattactga cactgaaaca tgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013299
VERSION     KHUT01013299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           53 c           83 g           46 t
ORIGIN      
        1 gacgtaccac ccgaacgtta ttcggaatca ctgggcttaa agagtccgta ggcggcccag
       61 aaagttggat gtgaaatacc tcggcctaac cgaggaacag cattcaaaac tactgggctt
      121 gagagataca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tatcttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013300             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013300
VERSION     KHUT01013300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c          102 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtccgt
       61 taagtccgag gtgaaaggtt cagcgctcaa cgcgaacagt gccttggaga ctgggggact
      121 tgagtgtggg agggggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctgcctgg cctaacactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013301             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013301
VERSION     KHUT01013301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           44 c           98 g           52 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttggt
       61 taagtcgggc gtgaaagctt taccactcaa cggaaagagg tcgctcgata ctggctgact
      121 agagtatggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgca gatatcagga
      181 agaacatcgg tggcgaaggc ggcttgctgg gccattactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013302             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013302
VERSION     KHUT01013302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           96 g           51 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agggagcgta ggcggttagt
       61 caagtccggt gtgaaagctt caccactcaa cgggaagagg tcactggaaa ctggctggct
      121 agagtaaggt agaggcaagc ggaattcctg gtgtagcggt ggaatgtgta gatatcagga
      181 agaacatctg tggcgaaggc ggcttgctgg gcctttactg acgctgaggc tcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013303             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013303
VERSION     KHUT01013303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcttga
       61 caagtttgat gtgaaatctc cgggcttaac ccggaaaatg cattgaatac tgtagaggct
      121 tgaggtttgg aggggggact ggaattctcg gtgtagcagt gaaatgcgta gatatcgaga
      181 ggaacaccag tggcgaaggc gagtccctgg acaacacctg acactgaggc acgaaagccg
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013304
VERSION     KHUT01013304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           86 g           50 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcatag
       61 aaagtcggat gtgaaatacc ggggctcaac cccggtgctg catttgaaac tcctaagcta
      121 gagtacagtt agggaaaaca gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggtgaagacg gttttctggg ctgtaactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013305
VERSION     KHUT01013305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           93 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcctga
       61 taagtctgct gtgaaagtcc agggctcaac cctggaatgc cggtggaaac tgtcaggcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013306
VERSION     KHUT01013306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agcgtatgta ggcggcttcg
       61 taagtcgggt gtgaaatccc ggggcttaac cccggaactg cactcgagac tgcaaggctt
      121 gagtatcgga gaggttggcg gaattcccag tgtagcagtg aaatgcgcag atattgggag
      181 gaacaccgat ggcgtaagca gccaactgga cgattactga cgctgagata cgaaagtatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013307
VERSION     KHUT01013307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           85 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggctgttctt
       61 taagtcttct gtgaaatcta cgggctcagc ctgtaacgtg cagaggatac tgaagagctt
      121 gagtgtcgga gaggatagtg gaattccagg tgtagaagtg aaattcgtag agatctggag
      181 gaacaccagt ggcgaaggcg actatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013308             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013308
VERSION     KHUT01013308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           63 c          109 g           31 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggccccg
       61 caggtcggta gtgaaagccc ccggctcaac cgggtggagg cgaccgaaac cgcggggctg
      121 gagggcgata gagggtggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgc ggcgaaggcg gccacctggg tcgaccctga cgctgaggcg cgaaggccgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013309
VERSION     KHUT01013309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           90 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggcggctttg
       61 caagtctggc gtgaaagccc ggggcttaac cccggatgtg cgttggaaac tgcattgctt
      121 gagttcggaa gaggagagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actctctggt ccgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013310
VERSION     KHUT01013310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggctcgt
       61 taagtcggac gtgaaagccc agggctcaac cctggaagtg cgttcgatac tggcaggctt
      121 gaatatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013311
VERSION     KHUT01013311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggagtta ctgggcgtaa agcgcgtgta ggtggtcaaa
       61 cgggtcgagt gtgaaagccc tcggcttaac cgaggaagtg cacgcgaaac tatttggctt
      121 gagtacggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccaga ggcgaaggcg gctctctaga ccgtaactga cactgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013312             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013312
VERSION     KHUT01013312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           96 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agagcccgca ggcggcttgg
       61 caagtttcag gtgaaatctc ccggcttaac cgggagggtg cccggaaaac tgccaggctt
      121 gaggcatgga gagggatgcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc catgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01013313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013313
VERSION     KHUT01013313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           95 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtggc
       61 caagtcagag gtgaaatcct ggagctcaac tccagaagtg cccctgatac tggtcatctc
      121 gagggagata ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013314             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013314
VERSION     KHUT01013314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           91 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtttgc
       61 caagtctgac gttaaagacg gaagctcaac ttccgcatgg tatcggaaac tggcaggcta
      121 gagtgtggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ccttgctggg ccaatactga cgctgaggaa cgaaagctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01013315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013315
VERSION     KHUT01013315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           51 c           78 g           52 t
ORIGIN      
        1 tacagaggcc ccaagcatta cccggaatca ctgggcgtaa agggtgtgta ggtggtcata
       61 ttagtcgagc gttaaatccc agggcttaac tctggaatcg cgttcgaaac ggtacgactt
      121 gagagcgtga gaggtgaatg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaagca attcactggc acgtttctga cactcaaaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013316             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013316
VERSION     KHUT01013316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           50 t
ORIGIN      
        1 tacagaggcc acgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgac
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg cttggtatac tggttgtctt
      121 gaggcactca ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaagaca gctcgctggg ggtgtactga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013317
VERSION     KHUT01013317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           92 g           49 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtctgat gttaaagacc ggcgctcaac gccgacacgg catcggaaac tggcaggctg
      121 gagtctggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaagcg ccttgctgga ccatgactga cgctgaggaa cgaaagctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01013318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013318
VERSION     KHUT01013318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggtttg
       61 taagtcagcg gtgaaatctc tctgcttaac agagaaactg ccattgatac tataagtctt
      121 gaattatccg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgctacg gattgattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013319
VERSION     KHUT01013319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           83 g           58 t
ORIGIN      
        1 tacatagggg gcaagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttag
       61 aaagtcggaa gttaaaacgt aaggcttaac cttgcggtgc tttcgatact tctatgactt
      121 gagtatgtca gaggttgatg gaattccagg tgtagcggtg aaatgcgcag atatctggag
      181 gaacaccagt agcgaaggcg gtcaactggg acattactga cgctgaggag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013320
VERSION     KHUT01013320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           87 g           55 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agggttcgta ggctggaaga
       61 caagttgggt gtgaaatcct cgggcttaac ctgagacgtg cattcaaaac tgtttttctt
      121 gaatgttgga gagggaagtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaagcg acttcctggc caaacattga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013321
VERSION     KHUT01013321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcggggctg
       61 caagtcagaa gtgaaatcca tgggcttaac ccatgaactg cttttgaaac tgtggctctt
      121 gagtatcgga gaggcaggcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcctgctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013322
VERSION     KHUT01013322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           43 c           99 g           50 t
ORIGIN      
        1 gacagagggt gcgagcgtta atcggagtga ctgggcgtaa agggcgcgta ggcggttgta
       61 agggtctgat gtgaaatgtc tgggcttaac ctgggatgtg cattggaaac tgtatgacta
      121 gagtctagga gaggagagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaaccctcgt ggcgaaggcg actctctggc ctaagactga cgctgaggcg cgacggcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013323
VERSION     KHUT01013323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccgg
       61 aaagtctggt gtgaaaactt ggggctcaac cctaagcctg cactggatac tatcgggctt
      121 gaatccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgattga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013324
VERSION     KHUT01013324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           87 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggtgatg
       61 taagtcagag gtgaaatccc agggctcaac cctggaatcg cctttgaaac tgcatcgcta
      121 gaatatgaga gagggtagtg gaattccgaa tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccgat ggcgaaggca gctacctggc tcattattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13477 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013325
VERSION     KHUT01013325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13477"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           84 g           57 t
ORIGIN      
        1 tacagggggt gcgagcgtta atcggattga ctgggcgtaa agagtgtgta ggtggtttaa
       61 tacgttggat gtgaaagact tgggctcaac ctaagaactg catccaaaac ggtttttcta
      121 gagtatagga gagaaaagca gaattcccgg tgtagaagtg aaattcgtag atatcgggag
      181 gaatgacagt ggcgaaggcg gctttttggc ctgatactga cactgagaca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01013326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013326
VERSION     KHUT01013326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           85 g           55 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatta ctgggcgtaa agcgcatgta ggtggcttag
       61 caagtcatat gtgaaagcct cgggctcaac ccgagaagtg catctgatac tgctgagctt
      121 gagtatggga gagggtagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gctacctggc ccaatactga cactgagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013327
VERSION     KHUT01013327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           88 g           53 t
ORIGIN      
        1 tacagaggtt gcaagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggtttga
       61 caagtcggtt gtgaaatccc cgggctcaac ctgggaattg cttccgggac tgtcaggcta
      121 gagatcggta gagggcggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccaat ggcgaaggca gccgcctggg cctgatctga cactgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013328
VERSION     KHUT01013328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           84 g           53 t
ORIGIN      
        1 gacgggggat gcaagtgtta tccggaataa ttgggcgtaa agcgtctgta ggtggtttac
       61 caagtctgtt gttaaaaatt agggcttaac cctgattcgg caacagaaac tagtaggctt
      121 gagtacggta ggggcagagg gaattctcgg tgtagtggtg aaatgcgtag atatcgagaa
      181 gaacaccaac agcgaaagca ctctgctggg ccgaaactga cattgagaga cgagagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01013329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013329
VERSION     KHUT01013329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           97 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgca gacggaactg
       61 caagtctgat gtgaaagccc ggggctcaac cgcgggactg catcggatac tgtagatctg
      121 gagtgccgga ggggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggcgaaggcg gcttgctgga cggcaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013330
VERSION     KHUT01013330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           86 g           47 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggttgtg
       61 taagtcggat gtgaaatccc ggagcctaac tccggaacgg cattcgaaac tacacagctc
      121 gagatctgga gaggtgagtg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaagcg actcactgga cagaatctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013331             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013331
VERSION     KHUT01013331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c          100 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggctcgg
       61 caagtctggt gtgaaatctt cacagctcac ctggaagagg tcactggaaa ctgctgagct
      121 tgaggtccgg agaggccgat ggaattcccg gtgtagcggt ggaatgcgta gatatcggga
      181 agaacaccag tggcgaaggc ggtcggctgg acggcacctg acgctgaggc gcgaaagtgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013332             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013332
VERSION     KHUT01013332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           46 c           81 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcatt
       61 gtaagcgggt agtgaaagcg tgtggctcaa ccatatacac attacccgaa ctgcaaagct
      121 agaggatgag agaggttatt ggaattccct gtgtaggagt gaaatccgta gatataggga
      181 ggaacaccga tggcgtaggc agataactgg ctcattcctg acactaaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013333
VERSION     KHUT01013333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           98 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgtcg
       61 caagtctgtg gtgaaagctc gatgctcaac atcgagtcgg ccacggatac tgcgaggctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtagctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013334             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013334
VERSION     KHUT01013334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           99 g           44 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgg
       61 taagtcgagt gtgaaatccc cgggctcaac ccggggcggc cattcgatac tgctatggct
      121 agagtccggt aggggagcgt ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013335
VERSION     KHUT01013335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           92 g           49 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacatt
       61 caagtcaggg gtgaaatccc ccagctcaac tggggaactg cctttgatac tgggtgtctt
      121 gagtaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaggcg gcatgctgga ccttcactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013336
VERSION     KHUT01013336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ttgggcgtaa agcgtgcgta ggcggttgtt
       61 caagacagat gtgaaacccc tgagctcaac ttaggaactg cgtttgtgac tggacggcta
      121 gagtgtgtta gagggaggcg gaattccatg tgtagcagtg aaatgcgtag agatatggag
      181 gaacaccgat ggcgaaggca gcctcctggg ataacactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013337             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013337
VERSION     KHUT01013337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           51 c           76 g           53 t
ORIGIN      
        1 tacagagacc tcaagcatta tccggattta ttgggcgtaa agggtccgca ggtggctttt
       61 cacgtcgctg gttaaatgtt ggggctcaac cctaacaccg ccaacgatac ggattagctc
      121 gagcatggga gaggtacgcg gaattactgg tgtagtagta aaatgcgtta atatcagtaa
      181 gaacaccaaa tgcgaaggca gcgtactgga acattgctga cactcaggga cgaaagcgtg
      241 gggagcgaaa ggg
//
LOCUS       KHUT01013338             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013338
VERSION     KHUT01013338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggaatca ctgggtttaa agggtgcgca ggcggcgcga
       61 taagtcagtt gtgaaatccc ccggctcaac cggcgggact gcgattgata ctgttgcgct
      121 tgaatcgggt tgaggcaggc ggaatgtggc atgtagcggt gaaatgctta gatatgccat
      181 agaacaccga ttgcgaaggc agcttgctgg gcccgtattg acgctgaggc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013339
VERSION     KHUT01013339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttctg
       61 taagaccgat gtgaaatccc cgggcttaac ctgggaactg cattggtaac tgcagagcta
      121 gagtgtgtca gagggaggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gcctcctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013340
VERSION     KHUT01013340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           90 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcatt
       61 taagtctggt ttgaaagcag gcggctcaac cgtctgatgt ggctggaaac tgagtgactt
      121 gaatgggtta gcggctgccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggcagccagg acctgattga cactgaggca cgagagcatg
      241 gggagccaac agg
//
LOCUS       KHUT01013341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013341
VERSION     KHUT01013341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgcgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggcgatctt
      121 gaggcactca ggggcgagtg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgat ggtgaaaaca gctcgctggg agtgttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013342
VERSION     KHUT01013342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           84 g           51 t
ORIGIN      
        1 tacgaggggt ctaagcgttg ttcggaatca ctgggcgtaa agcgggtgta ggtggctatg
       61 caagttagat gtgaaatccc cgggctcaac ccgggaagtg catctaaaac tacgtagctt
      121 gagtgcggga gagaaaagca gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaactccggt ggcgaaggcg gctttttggc ccagcactga cactcagacc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013343
VERSION     KHUT01013343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           41 c           89 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggggaga
       61 taagtccgtg gtgaaagacg atcgcttaac gattgcagtg ccatggatac tgtttttctt
      121 gaataacgtt gaggcgggtg gaattgaccg tgtagcggtg aaatgcatag atatggtcaa
      181 gaactccaat tgcgaaggca gcccgctggg cgttgattga cgctgaggca cgagagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013344
VERSION     KHUT01013344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           52 c           79 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggacttt
       61 taagtaggaa gtgaaaggtt ggagctcaac tccaacactg ctccctatac tggaagtctt
      121 gagtagcgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca gctttctgga cgcaaactga cgctcatgca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01013345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013345
VERSION     KHUT01013345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           87 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggcggaagag
       61 caagtcagat gtgaaatctc ggggcttaac cccgaacgtg cgtctgaaac tacttttcta
      121 gagtagcgga ggggagaggg gaatttcaca tgtaggggta aaatccgtag agatgtgaag
      181 gaacaccaga ggcgaaagcg cctctctggc cgctaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013346
VERSION     KHUT01013346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           79 g           56 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtgtgta ggcggtctga
       61 ttagtcagat gtcaaagact ggggctcaac cccaggaagg catttgaaac ggtcagacta
      121 gagaatgtga gagattgctg gaattcatgg tgtagtagtg aaatgcgttg atatcatgag
      181 gaacactcaa ggcgaaggca agcaattggc acatttctga cgctcagaca cgaaagcgtg
      241 gggagcgaat ggg
//
LOCUS       KHUT01013347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013347
VERSION     KHUT01013347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           95 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcacgta ggcggcccga
       61 taagtctgct gtgaaagtcc gaggctcaac ctcggaaggc cagtggatac tgtcgggcta
      121 gagttcggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat tgcgaaggca gctcgctggg acgatactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013348
VERSION     KHUT01013348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           82 g           58 t
ORIGIN      
        1 tacggggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtttgta ggtggctttg
       61 taagttaaat gtgaaagccc agggcctaac cctggaagtg catttaatac tgcagagctt
      121 gagtatgaga gaggaaggtg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacatcggc ggcgaaagcg gccttctggc ttaatactga cactgaaaaa cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013349
VERSION     KHUT01013349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           86 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggaatca ttgggcgtaa agagttcgta ggcggtttgt
       61 taagttagat gttaaatact gaggctcaac ctcagcacgg catttgatac tggcagactt
      121 gaatgcggta gaggtaaagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcggt ggcgtaagcg ctttactggg ccgtaattga cgctgaggaa cgaaagccag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013350
VERSION     KHUT01013350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacgaagggg gcgagcattg ttcggaatta ctgggcgtaa agggtgcgta ggcggatcga
       61 tcagtcagat gtgaaagccc agggctcaac cctggaactg catttgatac tgtcagtctg
      121 gagtattgga gaggagcgtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccagt ggcgtaggcg acgctctggc caaatactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013351
VERSION     KHUT01013351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcacgta ggcggggtca
       61 tatgtcgatt gtgaaagccc caggctcaac ctgggaactg cagccgaaac tgtgactctt
      121 gaatatctga gagggtgggg gaattgctgg tggagtagtg aaatacgtag agatcagcag
      181 gaacacctga ggcgaaggcg cctgcctggc agaatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013352
VERSION     KHUT01013352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           95 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttag
       61 caagtcaggt gtgaaatccc ggggctcaac tccggaagtg catttgaaac tgctaggcta
      121 gagtgttgta gaggatggcg gaatacccag tggagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gccatctggg caacaactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013353
VERSION     KHUT01013353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 caagtctctt gtgaaatctc cgggctcaac tcggagcctg caggagaaac tgcggtgctt
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013354             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013354
VERSION     KHUT01013354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           65 c          102 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggacgg
       61 cgcgtcggtc gctgaaagcc cccggctcaa ccggggaagc ggcgccgata cggccgacct
      121 ggagggacgt aggggggcct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggccctgg acgtctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013355
VERSION     KHUT01013355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 caagtcagat gtgaaagccc agggcttaac cctggaagtg catttgaaac tgcaagactt
      121 gagtgatgta gaggttggtg gaattcctag tggagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gccaactggg cattaactga cgctgaggcg cgagagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013356
VERSION     KHUT01013356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           90 g           54 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatta ctgggtgtaa agggagcgta ggcgggacgg
       61 caagtcagat gtgaaaacca tgggctcaac ctatggactg catttgaaac tgttgttctt
      121 gagtgaagta gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttactggg ctttaactga cgctgaggct cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013357
VERSION     KHUT01013357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           84 g           50 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcgggaagt
       61 taagtcagat gtgaaatccc ggggctcaac ctcggaactg catttgaaac tgattttcta
      121 gagggtaggc ggagaaaatg gaattccaca agtagcggtg aaatgcgtag agatgtggaa
      181 gaacaccagt ggcgaaggcg attttctagc ttattccaga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013358
VERSION     KHUT01013358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           44 c           77 g           56 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggtgggaaag
       61 gcagtcagat gtgaaatccc gaagctcaac ttcggaactg catctgaaac tccttttctt
      121 gagggtaggc ggagaaaata gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaatacctgt ggcgaaagcg attttctagc ttattcctga cactgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013359             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013359
VERSION     KHUT01013359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           38 c           76 g           62 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttaatg gtcgatgtca aactcaattt gtcggaagct gttaatacta ttctgctaga
      121 ggacaggaga ggttgatgga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaaa aggcgaaggc aatcaactat tctgttcctg acattgaggc atgaaagcgt
      241 gggtagcaaa ccgg
//
LOCUS       KHUT01013360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013360
VERSION     KHUT01013360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatga ctgggtgtaa agagcgtgta ggcggtctga
       61 taagtcagct gttaaagcgc ccggcttaac cgggcacatg cagttgaaac tgttgggcta
      121 gagtacggga gagaaaggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccttttggc ccgtaactga cgcttgagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013361
VERSION     KHUT01013361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggtggcctgt
       61 tatgttggcc gtgaaagccc atggctcaac catggaagtg cagccaaaac tggcaggcta
      121 gagttctgaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gccatctggg aagtgactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013362
VERSION     KHUT01013362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctgcg gtgaaatcct cccgcttaac gggagaacgg ccgcggatac tggcggcctc
      121 gaggaaggta ggggcaagcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg gcttgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013363             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013363
VERSION     KHUT01013363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           86 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgtta tccggattta ctgggcgtaa agcgtatcgt aggcggatgt
       61 gtgcgtggcg ggtttaagac tccagcccaa ctggaggaat gctcgtcata cggcgcatct
      121 tgaatttgtt cgagggaact ggaactctct gtggaggggt gaaatccgta gatatagaga
      181 agaacgccaa gggcgaaggc aggttcctag ggcaatattg acgctcatga acgacagcta
      241 ggggagcaaa aggg
//
LOCUS       KHUT01013364             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013364
VERSION     KHUT01013364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           81 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggtgtgt
       61 taagtgagat gcgaaatcct gtggctcaac catatggact gtttctcata ctggcatact
      121 tgaggatggt agaggcaagt ggaactggta gtgtagcagt gaaatgcgca gatattacca
      181 agaacaccaa tggcgaaggc agcttgctgg gccacacctg acactaaggc acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01013365             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013365
VERSION     KHUT01013365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           93 g           52 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctcgg
       61 taagtcgggt gtgaaatctc caagctcaac ttggagcttg catccgaaac tgctgtggct
      121 agagtctggt aggggaacac ggaatacctg gtgtagcggt gaaatgctta gatatcaggt
      181 ggaacaccag tggcgaaggc ggtgttctgg gccagtactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013366
VERSION     KHUT01013366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           65 c          113 g           27 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtga ctgggcgtaa agcgcgcgca ggcggccatc
       61 cgcgccggtc gtgaaagccc ccggctcaac cggggcacgt cgatcgggac ggggtggctg
      121 gagggtgtca gaggggggtg gaacgccgcg tggagcagtg aaatgcgtag agatgcggca
      181 gaacaccgga ggcgaaggcg gccccctggg gcacaactga cgctgaggcg cgaaggcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013367
VERSION     KHUT01013367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           97 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccga
       61 taagtccgct ctgaaagccc ggggctcaac cccgggaggc gggcggatac tgtcaggcta
      121 gagtccggaa gaggcaagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gcttgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013368
VERSION     KHUT01013368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           94 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggtggctcgg
       61 gtagtctggt gtgaaatctt tcggctcaac cggaagcggt cactggatac tctcgggctt
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acgtgactga cactgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013369
VERSION     KHUT01013369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           82 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggggaga
       61 taagtcagat gtgaaattcc aaagctcaac tttggagctg catctgaaac tgtctttctt
      121 gaggaatggc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacatctgt ggcgaaagcg gttttctagc ttaatcctga cgctgaggca cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013370
VERSION     KHUT01013370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatcgt
       61 taagtgcctt gtgaaatccc accgctcaac ggtggaactg cttggcatac tggcggtctt
      121 gagccggcta ggggcgactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggtcgctggg gccgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013371             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013371
VERSION     KHUT01013371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           89 g           49 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ttgggcttaa agggcgcgta ggcggcatcg
       61 taagttagag gtgaaatccc aacggctcaa ccgtggaact gcccctaata ctgcgatgct
      121 cgagggagat aggggcatgt ggaacttccg gtggagcggt gaaatgtgtt gatatcggaa
      181 ggaacgccgg tggcgaaagc gacgtgctgg atctcttctg acgctgaggc gcgaaagcta
      241 gggtagcgaa cggg
//
LOCUS       KHUT01013372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013372
VERSION     KHUT01013372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgaacgca ggcgattcat
       61 taagtcgggc gtgaaagccc cgggctcaac ctgggaattg cactcgatac tggtgaactt
      121 gagtctgaga gagggaagcg gaattcctgg tgtagaggtg aaattcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg gcttcctggc tcagtactga cgctcatgtt cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013373
VERSION     KHUT01013373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 taagttagat gtgaaagccc cgggcttaac ctgggaactg catttaagac tgcaaagctt
      121 gaggacgaga gaggaaagtg gaattgtgag tgtagaggtg aaattcgtag atattcacaa
      181 gaacaccagt ggcgaaggcg actttctggc tcgtatctga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013374             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013374
VERSION     KHUT01013374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           90 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgca ggcgggtcgt
       61 taagtcaggg gtgaaatccc gaggctcaac ctcggaactg cctttgatac tggcgacctt
      121 tgagtccggg agaggtgagt ggaactccga gtgtagaggt gaaattcgta gatattcgga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctcatgt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013375             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013375
VERSION     KHUT01013375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           48 c           99 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtccct
       61 taagtctgac gtgaaaggtt cagcgctcaa cgtgaacagt gcgttggata ctgggggact
      121 tgagtgcggg aggggagagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccga tggcgaaggc agctctctgg cctgtaactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01013376             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013376
VERSION     KHUT01013376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           82 g           55 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggatatg
       61 taagtcagat gtgaaattcc agggctcaac cttgggacag catttgaaac tgcattgtct
      121 agagggtagg cggagaaaac agaattccac aagtagcggt gaaatgcgta gatatgtgga
      181 agaataccgg tggcgaaggc ggttttctag cttattcctg acgctgaagc gcgaaagcaa
      241 ggggatcaaa cagg
//
LOCUS       KHUT01013377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013377
VERSION     KHUT01013377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           87 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtcgag
       61 taagttaatt gtgaaagtcc cgagcttaac ttggggattg cagttaatac tgcttggcta
      121 gagtgttgta gaggaaagcg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacatcgat ggcgaaggca gctttctgga caacaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013378
VERSION     KHUT01013378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggctaca
       61 caagtcgggg gtgaaatctt tcggcccaac cggaaaatag ctttcgatac tgtgtagctt
      121 gagggaggta ggggcacgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga tctccactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013379             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013379
VERSION     KHUT01013379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg tccggaatga ttgggcgtaa agcgtctgta ggtggctcgt
       61 aaagtctaat gtcaaatacc agggctcaac cttggaccgg cattggagta ctcacgagct
      121 tgagtacggt agaggcagag ggaattccaa gtggagcggt gaaatgcgta gagatttgga
      181 ggaacaccag tggcgaaggc gctctgctgg gccgaaactg acactgagag acgaaagctg
      241 ggggagcgaa tagg
//
LOCUS       KHUT01013380             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013380
VERSION     KHUT01013380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           92 g           51 t
ORIGIN      
        1 tacggggggg ggcaagcgtt gttcggaatt actgggcgta aagggtttgt aggtggcttg
       61 ttaagtcaga cgtgaaatcc ctcagcttaa ctggggaact gcgtctgata ctggcgagct
      121 tgagtgcagg agaggaacgc ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccgg tggcgaaggc ggcgttctgg actgcaactg acactgaaaa acgaaagcca
      241 gggtagcaaa cggg
//
LOCUS       KHUT01013381             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013381
VERSION     KHUT01013381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           95 g           47 t
ORIGIN      
        1 tacgtagggg cctagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcacgt gtgaaatctc cacgcttaac gtggaggcgc cacgtgaaac tgccgtgact
      121 tgagtccggt aggggggtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag tagcgaaggc ggcaccctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013382             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013382
VERSION     KHUT01013382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           66 c           99 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggcccgg
       61 cacgtttgtc gctgaaatcc cccggctcaa ccggggaacc ggcgcggata cgaccgggct
      121 ggaggggggt agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagctcctgg gccccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013383
VERSION     KHUT01013383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           91 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgat
       61 taagttatct gtaaaatccc cgggcttaac ctgggaaggt cagataagac tggttaactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ccaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013384
VERSION     KHUT01013384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           88 g           51 t
ORIGIN      
        1 gacgaaccgc ccgaacgtta ttcggaatca ttgggcttaa agggtgcgta ggcggcgctg
       61 taagtcgtgt gtgaaatctc tcggcttaac cgaggaactg cgcatgatac tgcagtgctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013385
VERSION     KHUT01013385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           96 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcc
       61 caagtccggg gtgaaatctt tcggcttaac cggaagacag ccccggagac tgggagtctc
      121 gagcgaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013386
VERSION     KHUT01013386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           97 g           45 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggcggcgcgt
       61 caagtcactg gtgaaagttt gcggcccaac cgtaaaattg ccggcgatac tggcgtgctt
      121 gagtgcgtga ggggttgggg gaacgagtgg tgtagcggtg aaatgcgtag agatcactcg
      181 gaaggccaga gccgaagggg cccaactggt acgcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013387
VERSION     KHUT01013387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           63 c          100 g           37 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgca ggcggcacag
       61 cacgttcgcg ctgaaatccc gtggcttaac cacggggcgc gtgcgaatac ggctgcgctc
      121 gaggcaggga gaggatgggg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacatccgt ggcgcaagcg cccatctggc cctgtcctga cgctgaggca cgagagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013388
VERSION     KHUT01013388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agcgtgtgta ggtggtttgt
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaactg cattcgatac tggcaggcta
      121 gagtacggta gagggaggcg gaactccggg tgtagcggtg aaatgcgtag atatccggaa
      181 gaacaccgat ggcgaaggca acctcctggg cctgtactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013389
VERSION     KHUT01013389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           85 g           48 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggctatg
       61 taagttggaa gtgaaagccc cgggctcaac ccgggaattg ctttcaaaac tacatagcta
      121 gagtatcgga gaggttggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgtaagcg gccaactaga cgataactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013390
VERSION     KHUT01013390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           95 g           43 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcgggtcga
       61 tgagtcaggg gtgaaatccc acggcccaac cgtggaagtg cctttgatac tgtcgatctc
      121 gagggggata ggggcgtgcg gaactcaggg tggagcggtg agatgcgtag atatcctgag
      181 gaacaccggt ggcgaaagcg gcgcgctgga tcccttctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013391
VERSION     KHUT01013391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttgg
       61 caagtcaggt gtgaaatccc ggagctcaac tccggacgtg cacttgaaac tgccaggctt
      121 gagtgtggta gaggatggcg gaatacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccatctggg ccacaactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013392
VERSION     KHUT01013392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           87 g           57 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggttgttatg
       61 taagtcggat gtgaaatccc tgggctcaac cgaggaagtg catccgaaac tatatggcta
      121 gaggatggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggc ggcgaaggcg gccttctggg ccattcctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013393
VERSION     KHUT01013393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           87 g           54 t
ORIGIN      
        1 tacagggggt gcaagcgtta ttcggattta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagtttgat gtgaaagccc tggactcaat tcaggaagtg catcgaatac tgctagacta
      121 gagtactgga gaggacaacg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga agcgaaggcg gttgtctgga cagttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013394
VERSION     KHUT01013394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           65 c          104 g           31 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggcggc
       61 caagtccggg gtgaaagccc acggcccaac cgtggcaggg cctcggagac tggccgcctc
      121 gagggaggta ggggcgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gcgcgctgga cctctgctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013395
VERSION     KHUT01013395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggtccta
       61 taagtctggg gtgaaagccc gttgctcaac aacggaacgg ccctggaaac tgtgggactt
      121 gagtacagac gagggtggcg gaatgggtgc tgtagcggtg aaatgcatag atagcatcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013396             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013396
VERSION     KHUT01013396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggccatg
       61 taagtcagaa gtgaaagccc agggcttaac cctggaagtg cttttgatac tgcgtggctt
      121 gagtgtggga gaggatagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccgga ggcgaaggcg gctatctggc ctaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13554 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013397
VERSION     KHUT01013397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13554"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggtcgcg
       61 taagtcggat gtgaaatctt ggggctcaac ctcaaaactg cattcgatac tgcgaggcta
      121 gaggactgga gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag agatcgtgag
      181 gaagaccaac ggcgaaggca gctctctgga cagtatctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013398
VERSION     KHUT01013398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           82 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggtggtcatg
       61 taagtcaaat gtgaaatgcc agggctcaac cttggattcg catgtgatac tgcgtgactt
      121 gagaactgga gagggatctt gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaaa ggcgaaagca ggatcctggc cagcttctga cactcaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013399
VERSION     KHUT01013399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           80 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggatggt
       61 taagtagaaa gtgaaaggtc ggagctcaac tccaacattg cttcctatac tggccatctt
      121 gagtctcgga gaggaaagtg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca actttctgga cgagaactga cgctcatgca cgaaagctaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01013400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013400
VERSION     KHUT01013400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           86 g           52 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggcggctttg
       61 caagtcggga gtgaaatccc agggcttaac cctggaattg ctttcgaaac tgcaaggctt
      121 gagtctcgga gaggttagcg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgtaagcg gctaactaga cgagtactga cgctcaagca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013401
VERSION     KHUT01013401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattag
       61 taagtcagtg gtgaaatctt tgggcttaac ccgaaaactg ccattgatac tattagtctt
      121 gaataccgtg gaggtcgacg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gttggctacg cgagtattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013402
VERSION     KHUT01013402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           95 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgga
       61 taagatgtgt gtgaaaaccc tgggcttaac ctgggacgtg catacaagac tgttcgacta
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggg ctgatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013403
VERSION     KHUT01013403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           54 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggcggcttga
       61 taagttggaa gtgaaatccc agggcttaac cttggaattg ctttcaaaac tattaggcta
      121 gagtctcgga gaggttagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgtaagcg gctaactaga cgagtactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013404
VERSION     KHUT01013404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           96 g           41 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggcttgt
       61 tacgtcgggt gtgaaagccc agggctcaac cctggcagtg cacccgaaac ggggaagctg
      121 gagtactgga gaggcgagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg actcgctggc cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013405
VERSION     KHUT01013405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaagta ggttgtctta
       61 taagtttact gtgaaatccc tgggctcaac ccaggaagtg cggtgagtac tgtaggacta
      121 gagtatcgga ggggtacgtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccaga ggcgaaagcg gcgtactgga cgataactga cactcaattg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013406
VERSION     KHUT01013406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcggatgta ggtggctttg
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cattcgatac tgcgaagctt
      121 gagtgctaga gaggttacta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccgga ggcgaaggcg ggtaaccagc tagacactga cactcagatc cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013407
VERSION     KHUT01013407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           86 g           50 t
ORIGIN      
        1 gacgaggggt gcaaacgtta ttcggaatga ttgggcgtaa agggtgcgta ggcggcgtgc
       61 taagtcaact gttaaatccc ccggcctaac cggggtcctg cagtagaaac tggcatgctt
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013408
VERSION     KHUT01013408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           79 a           41 c           79 g           54 t
ORIGIN      
        1 tacggagact gcaagcgtta ctcggattta ctgggcgtaa agggtgcgaa ggtggctaga
       61 tgtgtcagat gtgaaatctt gaagctcaac ttcaagcgtg cgtctgaaac tgtctagcta
      121 gagtattgga ggggtaagta gaattcctag tgtagcagtg aaatgcgtag atattaggag
      181 gaacaccaaa ggcgaaggca gcttactgga caataactga cactaaagca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01013409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013409
VERSION     KHUT01013409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          102 g           44 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgca ggcggccacg
       61 cacgtccgtt gtgaaatcgc tcggctcaac tgggcggggt cagcggatac ggcgcggctg
      121 gagcaagcta gggggcaatg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttgcctgga gctttgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013410
VERSION     KHUT01013410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           81 g           52 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggcggtcgtg
       61 aaagttagaa gtgaaatccc agggctcaac cttggaatgg cttttaaaac tccacgactt
      121 gagtattgga gaggttggcg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccaat ggcgtaggca gccaactgga caattactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013411
VERSION     KHUT01013411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccatt
       61 caagtcgagg gtgaaagcct gtggcttaac cacagaattg ccttcgatac tggatagcta
      121 gagaccggaa gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13570 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013412
VERSION     KHUT01013412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13570"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           40 c           71 g           62 t
ORIGIN      
        1 tacagacagt gcaagcgtta ttcggattga ttgggcgtaa aaggtatgta ggtggctatt
       61 taaacggagt gcgaaatact agagtataac tgtaggcggg cactttacaa ttatttagct
      121 tgagttaggg agaggtcggt agaatttctt ttgaaggggt aaaattccac gataattgaa
      181 cgaataccaa aagcgaaggc atccctccag ccttaactga cgctgaggta cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013413
VERSION     KHUT01013413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 tacagggggc tcgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggctcgc
       61 caagtgcttt gtgaaatccc cgggctcaac ccgggaacag cagggcaaac tggcgagctt
      121 gaagcaggta ggggccgaca gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaatgccgat ggggaagccg gtcggctggg cctgtcttga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013414
VERSION     KHUT01013414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           47 c           79 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggtgcgca ggcggggtgg
       61 caagtcaaat gtgaaatcct ggggctcaac ctcagaacag catttgaaac tactactctt
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttatacctga cgctcatgca cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013415
VERSION     KHUT01013415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           91 g           55 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttcgt
       61 taagtttggt gttaaagacc gaggctcaac ttcggggttg cactgaatac tggcggactg
      121 gagtatggta gaggcaagcg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgcaagcg gcttgctggg ccataactga cgctgaggaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01013416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013416
VERSION     KHUT01013416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtgggaatg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaattg catttgaaac tgtgtttcta
      121 gagtgaagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cttcaactga cactgaggcg cgaaagcgtg
      241 gggagcgaag agg
//
LOCUS       KHUT01013417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013417
VERSION     KHUT01013417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           82 g           53 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggagcg
       61 taagtcagat gtgaaatccc agggctcaac cctggaatag catttgaaac tgcgtttcta
      121 gagggtaggc ggagaaaaca gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaatacctgt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013418
VERSION     KHUT01013418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           81 g           62 t
ORIGIN      
        1 tacatagggg gcgagcgtta tccggattta ttgggcgtaa agcgtgcgta ggcggctaat
       61 taagtctgat gtgaaatttc acggcttaac cgtgaagggt cattggaaac tgattggctt
      121 gaatacagaa gaggtaagtg gaattccatg tgtagcggta aaatgcttag atatatggag
      181 gaacaccagt ggcgaaggcg gcttactggt ctgtcattga cgctgatgca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013419
VERSION     KHUT01013419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttag
       61 taagttggat gtgaaatccc tgggcttaac ctaggaactg catacaatac tgttagacta
      121 gagtgctgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013420
VERSION     KHUT01013420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggcgcgta ggtggttgag
       61 caagttagat gtgaaatccc tgggcttaac ctaggaatgg catctaaaac tgcttgactg
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013421
VERSION     KHUT01013421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           87 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttttg
       61 taagttaggt gtgaaattcc tgggcttaac ctggggactg cgcctaatac tgtaagacta
      121 gagtactgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013422
VERSION     KHUT01013422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           91 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgta
       61 taagttagct gtgaaattcc tgggctcaac ctgggatggt cagctaagac tgtatagcta
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013423
VERSION     KHUT01013423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggctggt
       61 tgtgtcgaat gtgaaagccc tcggcttaac cgaggaagtg cgttcgaaac tgactagctt
      121 gagtaccgga gaggaaggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg accttctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013424
VERSION     KHUT01013424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgtgta ggcgggtgtt
       61 taagtcagtg gtgaaatctc caggctcaac ctggaaactg ccattgatac tattcatctt
      121 gaattctgtt gaggtgggcg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccgat tgcgaaggca gctcactaaa cagcgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013425
VERSION     KHUT01013425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggcctat
       61 taagtcaggc gtgaaatccc cgggctcaac ctgggaactg cgtttgaaac tgataggctt
      121 gagagcggaa gaggaaagtg gaattcctag tgtagaagtg aaattcgtag atattaggag
      181 gaacaccaga ggcgaaggcg gctttctggt ccgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013426
VERSION     KHUT01013426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           98 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggggtcg
       61 caagtctgtg gtgaaatcct tcggcttaac cggagaactg ccgcggatac tgcgactctc
      121 gaggggtgta ggggcatgcg gaactcaggg tggagcggtg aaatgcgtag atatcctgag
      181 gaacgccggt ggcgaaagcg gcgtgctgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013427
VERSION     KHUT01013427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           47 c           98 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggcgtga
       61 tcagtcaggc gtgaaattcc tgggcttaac ctgggggctg cgcttgatac ggttgtgcta
      121 gaggatggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013428
VERSION     KHUT01013428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           97 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtttggg gtgaaatcct ccggctcaac cggagaacgg cctcaaatac tggcggtctc
      121 gaggggggta ggggcatgcg gaactatgcg tggagcggtg aaatgcgttg atatgcatag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013429
VERSION     KHUT01013429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           97 g           47 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggtgtaa agggtgcgta ggcggtcagt
       61 caagtctgat gtgaaatccc tcggctcaac tgaggaatgg cattggaaac tggctggcta
      121 gagtaccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gcttcctggc cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013430
VERSION     KHUT01013430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           94 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcccgca ggcggcttga
       61 aaagttttag gtgaaatctc ccggcttaac tgggagggtg cctagaaaac tatcaggctt
      121 gaggcaggga gagggatgtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgtcctga cgctcagggg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01013431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013431
VERSION     KHUT01013431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ctgggcataa agcgcatgta ggcggctcga
       61 tcagtcgggt gtgaaagccc ctggctcaac cagggaactg cattcgatac tgtcgggctc
      121 gagggtggaa gaggagagtg gaactcgtag tggagcggtg aaatgcgtag atattacgag
      181 gaacaccggt ggcgaaagcg actctctggt ccattcctga cgctgagatg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013432
VERSION     KHUT01013432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 tacgaaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcgtgta ggcggcttgg
       61 caagtcgggt gtgaaatccc tcagctcaac tgaggaactg cgcccgaaac tgccttgctt
      121 gaggcctgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg actctctgga caggttctga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013433
VERSION     KHUT01013433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           51 c           80 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgagcgtaa agaatgcgta ggtagtcgga
       61 taagtccgat gtgaaatctc cgagcctaac tcggaaactg catcggatac tattcggcta
      121 gagggttgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013434
VERSION     KHUT01013434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           96 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggctttt
       61 caagtcgggc gtgaaagctc cgggcttaac ccggaaatgt cgctcgatac tgttgagcta
      121 gagtgtggaa gaggctactg gaattccgtg tgtagcggtg aaatgcgtag atatacggag
      181 gaacaccagc ggcgaaggcg ggtagctggg ccaacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013435
VERSION     KHUT01013435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           59 c          104 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggcgggt
       61 taagtggggt gtgaaagccc ggggctcaac cccgggacgc cgctccaaac tggccggctt
      121 gaggacggaa gaggggagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctccctggt ccgtccctga cgctgagacg cgaaagcgcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013436
VERSION     KHUT01013436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggtcta
       61 taagtagatt gtgaaatccc tgggctcaac ccgggacgtg cagtctaaac tgtagatctt
      121 gaatgtctga gaggatggcg gaattcccgg tgtagtagtg aaatacgtag atatcgggaa
      181 gaacacctga ggcgaaggcg gctatctggc ggaacattga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013437             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013437
VERSION     KHUT01013437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtcaggg gtgaaagcct tgggcttaac tcaagaagtg cccttgaaac ggtgagacta
      121 gagtcctgga gaggctagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gctagctgga caggtactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013438
VERSION     KHUT01013438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           86 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgaa
       61 taagttaggt gtgaaagtcc tgggcttaac ctggggactg cgcctaatac tgtttaacta
      121 gagtactgta gaggaaggtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacatctgt ggcgaaggcg accttctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013439
VERSION     KHUT01013439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          100 g           47 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtcttg
       61 tttgtctgct gtgaaagtcc acggctcacc cgtggagagt cggtagaaac ggcaagactt
      121 gaggctggga ggggttggtg gaattccctg tgtagcggtg aaatgcgtag atacggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013440
VERSION     KHUT01013440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           88 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggcgtgta ggtgggagat
       61 taagtcagat gtgaaagccc gcagcttaac tgcggtactg catctgaaac tagtcttctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013441
VERSION     KHUT01013441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtgccg
       61 taagtcctat gtgaaagccc tgggctcaac ccaggaagtg cgtaggatac tgcgggactg
      121 gagtgacgga gaggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgttaactga cgctgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013442
VERSION     KHUT01013442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           82 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggtaagg
       61 caagtcaggt gtgaaatccc ggagcttaac tccggaactg cacttgaaac tgcctttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttacaactga cgctgatgcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013443
VERSION     KHUT01013443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           76 g           60 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggagcgta ggtggactga
       61 taagtcagtg gtgaaagccg ttagcttaac taacgaattg ccattgatac tgttggtctt
      121 gagtacagtt gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cactgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01013444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013444
VERSION     KHUT01013444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           55 c           77 g           52 t
ORIGIN      
        1 tcccagcggt ccaagtcgca accacaatta ttgggtctaa aacatccgta gcttgcttga
       61 taagtctcct gtgaaatccg gcctcttaag ggtcgaagag catgagatac tgtcgggctt
      121 gggaccggta gaggtaagaa gtacggtcgg ggtaccggta aaatgggttg atcccgtctg
      181 gactaacaac agcgaaggca tcttaccagc acggatctga cagtaaggga tgaaggctag
      241 gggcgcaaaa ggg
//
LOCUS       KHUT01013445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013445
VERSION     KHUT01013445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          102 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtctgt
       61 cgcgtccgtt gtgaaagctc ccggctcaac tgggagaggt cttcggatac gggcagactc
      121 gagggaggca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctttcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013446
VERSION     KHUT01013446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           97 g           38 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccgt
       61 caagtcgagg gtgaaatccc ccagctcaac tggggaagtg cctccgatac tggcggcctc
      121 gagggggata ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgtgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013447
VERSION     KHUT01013447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggattcc
       61 caagtcggtg gtgaaatctt tcggcccaac cggaaaaggg ccgccgatac tgggagtctc
      121 gagggaggta ggggtcgagg gaactggtgg tggagcggtg aaatgcgtag atatcactag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013448
VERSION     KHUT01013448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcatat
       61 caagtcagct gtgaaatctc cgggcttaac tcggaactgt cagctgaaac tgatgtgctg
      121 gagtgcagaa ggggcaaccg gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013449
VERSION     KHUT01013449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggctctc
       61 caagttaggt gtgaaagccc tgggcttaac ccaggaactg cgcttaatac tggagagcta
      121 gaggttggta gaggaaagtg gaactcctag tgtagaggtg aaattcatag atattaggaa
      181 gaacaccaga ggcgaaggcg actttctggg ccaatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013450
VERSION     KHUT01013450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           50 c           74 g           49 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agagcgaaaa gccggttgcg
       61 ttagtctgat gtaaaaacat ggggcccaac ttcatgaccg cattggaaac ggcgcaactt
      121 gagcagataa gaggagagca gaacatatgg tgtaggggtg aaatccactg atatcatatg
      181 gaataccaaa agcgaaggca gctctctagt gtcttgctga cggtgcatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01013451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013451
VERSION     KHUT01013451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           90 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggccact
       61 taagtctggt ttgaaagctg gtggctcaac catcagatgt ggctggaaac tgggtggctt
      121 gaatgggttg gccgtcgctg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggcgacgagg actctattga cactgaggca cgagagcatg
      241 gggagccaac agg
//
LOCUS       KHUT01013452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013452
VERSION     KHUT01013452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgtgca ggcggattgt
       61 taagtcgggg gtgaaatcct ggagctcaac tccagaacgg ccttcgatac tggcaatctc
      121 gagtccggaa gaggtgggtg gaattcctag tgtagaggtg gaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013453
VERSION     KHUT01013453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           61 c           79 g           43 t
ORIGIN      
        1 tacagagacc ccgagcatta cccggaatta ctgggcgtaa aggacgagta gccggtcgtg
       61 ttagtccgat gtcaaaccct ggggcccaac cccatgtctg catcggaaac ggcatgactc
      121 gagcgagtga gaggtgagcg gaacgtacgg tgtaggggtg aaatccactg atatcgtacg
      181 gaacaccaaa agcgaaggca gctcactagc gctctgctga cggtcaatcg tgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01013454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013454
VERSION     KHUT01013454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 taagtctcgt gtgaaatccc tcggctcaac tgaggaactg cacgggatac tgcttggctt
      121 gaattcggga gagggacgcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga ccgacattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013455
VERSION     KHUT01013455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           95 g           49 t
ORIGIN      
        1 tacggggggg gctaacgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggttcgg
       61 taagtctagc gtgaaatctc accgctcacc ggtgaaacgg cgctggaaac tgtcgagctc
      121 gaggatggaa gaggtaggtg gaattccagg tgtagcggtg gaatgcgtag atatctggaa
      181 gaacactcgt ggcgaaggcg gcctactggt ccatttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013456
VERSION     KHUT01013456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          102 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcggtcatc
       61 gacgtgccgt gtgacagacg ccggctcaac cggcgtaggc cagggtacac gagatgactg
      121 gaggcagaga gagggatgtg gaatacctgg tggagcggtg aaatgcgtag agatcaggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc tctgttctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01013457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013457
VERSION     KHUT01013457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           93 g           46 t
ORIGIN      
        1 gacagaggtt gcaaacgttg ttcggaatta ctgggcataa agcgcacgta ggcggtccgc
       61 taagtcgggc gtgaaatccc ccggctcaac cggggaattg cgcctgatac tggcgggctt
      121 gaggttggta ggggtgagcg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccaattctga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013458
VERSION     KHUT01013458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           90 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcataa agggcgcgta ggcgggccga
       61 taagtcaggg gtgaaatcct ccagctcaac tggagaactg cctctgatac tgacggcctc
      121 gagtaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcatgctgga ccttcactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013459
VERSION     KHUT01013459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           53 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ctgggtgtaa agggtgcgta ggtggcggta
       61 ttagtctttt gtgaaagccc cgagctcaac ttgggaaacg caaaagaaac ggtactgcta
      121 gagagtgtga ggggtctagg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca ctagactggc acatttctga cactcaagca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013460
VERSION     KHUT01013460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          100 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtccggg gtgaaagccc cccgctcaac gggggaatgg ccttggatac tggcggcctg
      121 gaggaaggta ggggcagatg gaactctcgg tggagcggtg aaatgcgtag atatcgggag
      181 gaacgccggt ggcgaaagcg atctgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013461
VERSION     KHUT01013461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggtgatt
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaatgg catctcatac tgagtgacta
      121 gagtattgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga caaatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013462
VERSION     KHUT01013462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           46 c           98 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgct gtgaaatccc ggggcttaac cccgggtgtg cagtgggtac gggcagactt
      121 gagtgcagta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atattaggaa
      181 gaacaccgat ggcgaaggca ggtctctggg ctgttactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013463
VERSION     KHUT01013463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           86 g           48 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggctcta
       61 taagtcagat gtgaaattcc ggggctcaac cccggagcgg catccgaaac tatagaacta
      121 gagtgcagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggtgaagacg gttttctggg ctgtaactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013464
VERSION     KHUT01013464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           83 g           55 t
ORIGIN      
        1 tacgagtgcc tcaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggtcatg
       61 ttagtcttct gttaaatttc ccggcttaac cgggaacctg cggaggaaac ggcatgactt
      121 gaggatgcga ggggtctgcg gaactcatag tgtagcggtg aaatgcgttg atattatggg
      181 gaacaccaaa agcgaaggca gcagactgga gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013465
VERSION     KHUT01013465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtgggttga
       61 tgtgttggct gttaaagccc cgagcttaac ttgggaaagc cagccaaaac tgtcagccta
      121 gagtataata gaggagagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actctctgga ttaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013466
VERSION     KHUT01013466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           88 g           51 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggcatat
       61 caagtctggt gttaaagccc gaggctcaac ctcggatcgg cactggatac tgataagcta
      121 gagtgcagta gaggcaaggg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgtaagcg ccttgctggg ctgttactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01013467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013467
VERSION     KHUT01013467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           73 g           63 t
ORIGIN      
        1 tacgaaggat ccaagcgttg tccggattta ctgggtttaa agggtgcgta ggcggactat
       61 taagtcagtg gtgaaagccg gtagcttaac tatcgaattg ccattgaaac tgatagtctc
      121 gagtatagtt gaggtagttg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccgat tgcgaaggca gattactaaa ctataactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013468
VERSION     KHUT01013468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           86 g           58 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggatatg
       61 taagttagat gtgaaagacc tgggcttaac ctgggatggt catttaatac tgtgtatcta
      121 gagtatggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcaat ggcgaaggca gctacctgga ctgatactga cactgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013469
VERSION     KHUT01013469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggtctgt
       61 taagtttgac gtgaaagctc ccggctcaac tgggagaggt cgttgaagac tggcagactt
      121 gaggcgatga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg gcaccctagc gttagcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013470
VERSION     KHUT01013470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           82 g           57 t
ORIGIN      
        1 tacggaggat tcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggttcga
       61 taagttagag gtgaaatccc ggggctcaac tccggcactg cctctgatac tgtcgggcta
      121 gagtttagtt gcggtaggcg gaatgtatgg tgtagcggtg aaatgcatag agatcataca
      181 gaacaccgat tgcgaaggca gcttaccaaa ctacgactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013471
VERSION     KHUT01013471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           86 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagcgta ggtcgcttag
       61 caagtcagtg gtgaaagccc agggctcaac tctggaactg ccattgaaac tgctaagctg
      121 gaatctcaga ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga tgagtattga cactgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013472
VERSION     KHUT01013472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           83 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgagtgta ggcggttact
       61 taagtctgat gtgaaagccc agggctcaac cctggaagtg cattggatac tgggtaacta
      121 gagggcgata gaggagaacg gaattaatgg tgtaggggtg aaatccgtag atatcattag
      181 gaacaccgaa agcgaaggct gttctctggg tcgcaactga cgctaagact cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013473
VERSION     KHUT01013473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttggt
       61 taagtgaggt gtgaaagtcc cgggctcaac ctgggaaggt cacttcatac tggtcgactt
      121 gagtagggta gagggaggtg gaatttccgg cgtagcggtg aaatgcgtag atttcggaag
      181 gaacaccggt ggcgaaggcg gcctcctgga cccatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013474
VERSION     KHUT01013474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           84 g           57 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagtgcgta ggcggatatt
       61 taagtcatat gtgaaatccc cgggcttaac ctgggaattg catctgatac tgggtatcta
      121 gagtatggta gaggacagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaag
      181 gaacaccagt ggcgaaggcg actgtctggg ccaatactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013475
VERSION     KHUT01013475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           90 g           43 t
ORIGIN      
        1 tacagaggcc acaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggcccgc
       61 caagtgtctt gtgaaatccc ggagctcaac tccggaacag cagggcatac tggcgggctc
      121 gagaaaggta agggtcgctg gaactgtagg tggagcggtg aaatgcgtag atatctacag
      181 gaacgccgat ggtgaagacg agcgactggg ccttttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013476
VERSION     KHUT01013476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           81 g           57 t
ORIGIN      
        1 tacggaggat ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcaggg gtgaaagacg gtagctcaac tatcgcagtg cctttgatac tgcagggctt
      121 gaatatacta gaggcaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttgctatg gtataattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013477
VERSION     KHUT01013477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggtgtaa agggcgcgta ggcgggagta
       61 caagtcagat gtgaaatgct gaggcttaac ctcagagctg catttgaaac tgtacttctt
      121 gagtatcgga gaggcaggcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcctgctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013478
VERSION     KHUT01013478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcctgt
       61 taagtcggtt gtgaaagccc atggctcaac catggaaggg cgtccgaaac tggcaggcta
      121 gagtcccgaa gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccaga ggcgaaggcg gccatctggg agggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013479
VERSION     KHUT01013479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           54 c           83 g           45 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattca ttgggcgtaa agagcacgca ggcggtttgg
       61 caagtcggat gtgaaaacca gaagctcaac ttctggcctg catccgaaac tgcccgacta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatacgcag atatcaaaag
      181 gaacaccgat ggcgaagacg gatctctgaa ccttacctga cgctcaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013480
VERSION     KHUT01013480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggatatt
       61 taagttggat gtaaaaaccc tgggcttaac ctaggactgt catacaagac tggatatctg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013481
VERSION     KHUT01013481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           56 c          103 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ttgggcgtaa agggcgcgta ggtggcggga
       61 gaagcgtgcg gtgaaagctc ggggctcaac cccgagtctg ccgtgcgaac tctcttgctt
      121 gagcactgta gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg cagtggctga cactgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013482
VERSION     KHUT01013482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           90 g           46 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggtgagg
       61 caagttcggt gtgaaagctc caggctcaac ctggaaatgg cactgaaaac tgctttgctt
      121 gaggtttgga ggggggactg gaattctcgg tggagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01013483             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013483
VERSION     KHUT01013483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           82 g           53 t
ORIGIN      
        1 tacgtggggt gcaagcgtta tccggattta ctgggcgtaa agcgtaccgt cggcgacttg
       61 ataagtttct tgtttaagcc caaggctcaa ctttggaaca gcaggaaaaa ctatcaagat
      121 tgagtctgtt cggggaagtt ggaattctcg gtggaggggt gaaatccgta gatatcgaga
      181 ggaacgccaa gcgcgaaggc agacttctag gacacgactg acgctcaggg acgaaagctc
      241 ggggagcaaa aggg
//
LOCUS       KHUT01013484             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013484
VERSION     KHUT01013484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           94 g           53 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggattta ctgggcgtaa agagttcgta ggcggttgtg
       61 taagtctggt gtgaaatctt caccgctcaa cgggaagagg tcactggaaa ctgcgcaact
      121 tgagtatggt agaggcaagc ggaattcccg gtgtagcggt ggaatgtgta gatatcggga
      181 agaacacctg tggcgaaggc ggcttgctgg gccattactg acgctgagga acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013485             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013485
VERSION     KHUT01013485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           64 c          100 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta gacgggcgac
       61 cgcgtcgatg gctgaaagcc cccggctcaa ccggggaacg ggcctcgata cgggtcgcct
      121 cgagtggagg agggggacct ggaactgccg gtggagcggt gaaatgcgtt gagatcggca
      181 ggaacgcctg tggcgaaagc gaggtcctgg actcccactg acgttgaggc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013486             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013486
VERSION     KHUT01013486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          103 g           41 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggcttct
       61 taagtctgac ggtgaaatgc cggggctcaa ccccgtgccc gccgcggata ctggggagct
      121 ggagacgagt agaggcaagc ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg aggcgaaggc ggcttgctgg gcttggtctg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01013487             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013487
VERSION     KHUT01013487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           63 c           99 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggaggg
       61 aacgtcggcc gctgaaatcc cccggcttaa ccggggaagt ggcgccgata cggccctcct
      121 ggagggacgt agggggagct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg cggcgaaagc gagctcctgg acgtctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013488
VERSION     KHUT01013488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtccggg gtgaaatccc ccagcttaac tggggaagtg ccctggatac tggtggcctc
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccggt ggcgaaagcg aggtgctgga ccctttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013489
VERSION     KHUT01013489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctttg
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaagtg cactcgaaac tgcaaagctt
      121 gagtatcgga gaggttggta gaattctcgg tggagaggtg aaattcgtag atatcgaggg
      181 gaataccagt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013490
VERSION     KHUT01013490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggctagg
       61 taagtccgct gtgaaaactc gaggctcaac ttcgagctgt cggcggaaac tatctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013491
VERSION     KHUT01013491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttta
       61 aaagtggcgt gtgaaatacc cgggcttaac ctgggggggt cacgtcatac tgtaagacta
      121 gagtatagga gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacacccgt ggcgaaggcg gctctctggc ctaatactga cactgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013492
VERSION     KHUT01013492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           96 g           47 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtccgt
       61 tacgtctgct gtgaaaactc ggggctcaac cccgagcctg cagtggatac gggcggacta
      121 gagtgcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctgga ccgatactga cactgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013493
VERSION     KHUT01013493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           91 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggctgat
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaacgg cgtttgaaac tggtcagcta
      121 gagtttggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ctaaaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013494
VERSION     KHUT01013494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggtggcggga
       61 taagcgtgtg gtgaaagctc ggagctcaac tccgagtctg ccatgcggac tgtcttgctc
      121 gagcgtagta gaggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggg ctactgctga cactgatgca cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013495
VERSION     KHUT01013495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           82 g           56 t
ORIGIN      
        1 tacgaaggtg gcgaatgttg ctcggattca ctgggcgtaa agcgtcggta ggtggtgttt
       61 taagttagag gtgaaagccc cgcgctcaac gtgggaactg cctttaaaac tgggacactt
      121 gagagtatca gaggatggcg gaataactgg tgtagaggta aaattcatag atatcagttg
      181 gaacacccat ggcgaaggca gctatctggg atatttctga cactgagcga cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013496
VERSION     KHUT01013496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           98 g           48 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtccat
       61 cgcgtcgaat gtgaaatccc ggggctcaac tccgggcctg cattcgatac gggtgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013497
VERSION     KHUT01013497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           87 g           54 t
ORIGIN      
        1 tacgtagggt ccgagcgtta tccggtatta ttgggcgtaa agagcgcgta ggcggtttgg
       61 taagacaatg gttaaatctc catgctcaac atggaagctg ccgttgtgac tgccaaactt
      121 gacgatagca ggggcaagtg gaattctcgg tgtaggggtg aaatccgttg agatcgagaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctatatgtga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013498
VERSION     KHUT01013498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggtggccatg
       61 tatgtcagga gtgaaagccc tcggcttaac cggggaagtg cttttgaaac tgcatggctt
      121 gaatactgga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga cagatattga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013499
VERSION     KHUT01013499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           93 g           41 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgca ggcggtttcg
       61 cgagtccgac gtgaaagccc ggggcccaac cccggaagtg cgttggatac tacgaagctt
      121 gagtgctgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagacactga cgctgagacg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01013500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013500
VERSION     KHUT01013500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggctaag
       61 taagtctgat gtgaaagccc tgggcttaac ctgggaatag cgttggaaac tgtttagcta
      121 gagtcggaca gagggcggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacccgt ggcgaaggcg gccgcctggg accagactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013501
VERSION     KHUT01013501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggttcgc
       61 taagtctcat gtgaaatccc ccggctcaac cggggaattg cgcgagatac tggcgagctt
      121 gagatcggta ggggtgagtg gaactccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgacactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013502
VERSION     KHUT01013502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 tacagagagg gcaagcgttg tccggaatga ctgggcgtaa agcgcgcgca ggcggtttct
       61 taagttggat gtgaaatccc cgggctcaac ctgggaactg cattcgatac tggggaacta
      121 gaggccgaga gaggaaagcg gaatgacgag tgtagaggtg aaattcgtag atattcgtcg
      181 gaacaccaga ggcgaaggcg gctttctggc tcggacctga cgctcaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013503
VERSION     KHUT01013503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           84 g           56 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggaatta ttgggcgtaa agagaacgta ggtggtttct
       61 taagtttggg cttaaagact ggagcttaac tccaggactg gctcgaaaac tgggagacta
      121 gaggatgtca gaggaaagtg gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaacaccagt ggcgaaagcg actttctggg acattcctga cactgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01013504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013504
VERSION     KHUT01013504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggattcc
       61 caagtccggg gtgaaatctt tcggctcaac cggaaaatag ccctggatac tgggggtctc
      121 gagtgaggta ggggcagctg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013505
VERSION     KHUT01013505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c          102 g           40 t
ORIGIN      
        1 gacggaaggt gcaagcgttg ctcggaatca ttgggcgtaa agggtgcgta ggcggcttgg
       61 tcagtcgggt gtgaaagccc tgggctcagc ccgggaaggg cactcgaaac tgccgagctg
      121 gagtgccgga ggggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013506
VERSION     KHUT01013506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctcg
       61 tttgtccgag gtgaaagccc cgagcttaac ttgggaagtg cctcggaaac ggcgagacta
      121 gagtcttgga gagggtcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaataccaga ggcgaaggcg gcgacctgga caagttctga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013507
VERSION     KHUT01013507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           91 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgggg
       61 aaagtcagat gtgaaatccg ggggctcaac ccccgaatgg catccgatac ttccttgctg
      121 gagggttgta gggggatctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgt ggcgaaggcg agatcctggg caacttctga cgctgatgca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013508
VERSION     KHUT01013508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggccacg
       61 acagtcttct gtgaaatcta cgggcttagc ctgtaacgtg cagaggatac tacgtagctt
      121 gagtgtcgga gaggatagtg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg actatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013509
VERSION     KHUT01013509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           55 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcacg
       61 caagtcgggg gtgaaatcct ccagctcaac tggagaacgg ctttcgatac tgcgtgtctt
      121 gagggaggta ggggcaggtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acctgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013510
VERSION     KHUT01013510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgtta cccggaatca ctgggcgtaa agagtgcgta ggtggctagg
       61 taagtcttct gtgaaagctc ccggctaaac tgggagaggt caggggaaac tacctggctc
      121 gagggcagta gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg ctgttcctga cactgaggca cgagagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01013511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013511
VERSION     KHUT01013511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           86 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgtc ggcggtttgg
       61 caagaaaggt gtgaaatccc agggcttaac cttggaactg cacttttaac tgccaggcta
      121 gagtatgtca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataatactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013512
VERSION     KHUT01013512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           91 g           51 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgatg
       61 taagtcgggc gtgaaatccc ggggctcaac cccggaactg cgttcgatac tgctttgctt
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccggt ggcgaaggcg ggtctctaga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013513
VERSION     KHUT01013513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           93 g           42 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccgg
       61 caagtcgggt gtgaaagccc tcggcttaac cgaggaagtg cattcgaaac taccggactc
      121 gagtactgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013514
VERSION     KHUT01013514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttgg
       61 caagtcggtt gtgaaatccc cgggcttaac ccgggaaatg cgaccgaaac tacctagcta
      121 gagtctcgga gaggaaggca gaattcccag tgtaggggtg aaatccgtag atactgggag
      181 gaataccggt agcgaaggcg gccttctgga cgaagactga cgctcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013515
VERSION     KHUT01013515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           96 g           48 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agcgtgcgta ggcggtgatc
       61 caagtctggt gtgaaatctc ccggcttaac tgggagggtg cgccggaaac tggatcgctg
      121 gagtgcggga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgc ggtgtagacg gcttactgga ccgccactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013516
VERSION     KHUT01013516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           93 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctcct
       61 caagtcggat gtgaaagccc tgggctcaac ccaggaagtg catccgaaac tgtggagctt
      121 gaatgccgga ggggcccaga gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaataccagt ggcgaaggcg ctgggctgga cggtcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013517
VERSION     KHUT01013517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           53 c          102 g           45 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcgt
       61 cgcgtcggct gtgaaaacct gccgctcaac ggtaggcgtg cagctgatac gggcgggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013518
VERSION     KHUT01013518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtctggt gtgaaatctc ggggctcaac cccgagcgtg cactggatac tgcctagctt
      121 gagtccggga ggggagagcg gaattcctag tgtagcggtg aaatgcgcag atattaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013519
VERSION     KHUT01013519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           92 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcgggtagg
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tgcctgtctt
      121 gagtgcggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013520
VERSION     KHUT01013520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           46 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatca ctgggcgtaa agcgcgtgta ggcggtctcc
       61 taagtttggt gtgaaagccc ggggctcaac tccggaagtg catcggatac tgggagacta
      121 gagtaccgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccctctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013521
VERSION     KHUT01013521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           85 g           53 t
ORIGIN      
        1 tacctgcagc ccgagtggtg ctcgttatta ttgggtctaa aacgtccgta gtcggttgag
       61 tagatgcccg ggtaaatcgc agcgcttaac gttgcgaatt ccgggtagac cgctcgacta
      121 gggattggga gaggtgcata gtactctgag ggtaggggta aaatcctgta atccttgggg
      181 gactaccagt ggcgaaggcg atgcactaga acaagtccga cgatcaggga cgaagccctg
      241 gggcgcaaac ggg
//
LOCUS       KHUT01013522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013522
VERSION     KHUT01013522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           94 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccgg
       61 tcagtcggtt gtaaaagtca agggctcaac ccttgaaagc cggccgatac tgccgggcta
      121 gagtccggaa gaggtgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcactggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013523
VERSION     KHUT01013523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           91 g           53 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtttag
       61 caagtcactt gtgaaatctt tgggcttaac ccaaaacggt caagtgatac tgttagacta
      121 gagtgcagaa ggggcaactg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctggg ctgacactga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013524
VERSION     KHUT01013524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           88 g           50 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcggacagg
       61 caagtcagcg gtgaaatctc acggctcaac cgtgaaactg ccgttgatac tgcttgtctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccggt ggcgaaggcg gtcaactggc ccgtaactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013525
VERSION     KHUT01013525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           46 c           96 g           53 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcttc
       61 taagtctggt gtgaaatctc ccggctcaac tgggagggtg cgccggaaac tgggaggcta
      121 gagtgtggga tgggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctctctgga ccgcaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013526
VERSION     KHUT01013526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           85 g           53 t
ORIGIN      
        1 gacagaggat gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggtggtcttg
       61 tgtgtctggt gtgaaagccc ttagctcaac taaggaagtg cgctagaaac tgcaagactt
      121 gaattcggga gaggatggta gaattctcag tgtagaggtg aaattcgtag atactgggag
      181 gaacaccggt ggcgaaggcg accatctgga ccgacattga cactaaagtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013527
VERSION     KHUT01013527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           86 g           47 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggtgtccttc
       61 cacgtctggt gtaaaagcac ggagctcaac tccgtgtacg tgccggaaac gagagggatt
      121 gagtcattca gaggcatctg gaatgtcgtg tgtaggggta aaatccgtag atccacgatg
      181 gaacgccaaa agcgaaggca ggatgctggg aatgtactga cactcagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01013528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013528
VERSION     KHUT01013528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          101 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggtcagc
       61 taagtctggt gtcaaagccc acggcttaac cgtggagggg cactggaaac tggctgactt
      121 gagtgcagga gagggaagcg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gcttcctggt ctgtcactga cgctgaggcg cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013529
VERSION     KHUT01013529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           39 c           95 g           52 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatga ttgggcgtaa agggtgtgta ggcggtgggt
       61 taagacggat gtgaaatttc tgggctcaac ctggagatgg catacgtgac tggcgcactg
      121 gaatatagca gagggaggtg gaattccaca tgtagcagtg aaatgcgtag agatgtggag
      181 gaacatcgat ggcgaaggca gcctcctggg ttaatattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013530
VERSION     KHUT01013530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           83 g           60 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcctat
       61 taagtcagtg ctgaaatcct ccggcttaac cggagatgtg gcattgatac tgatgggcta
      121 gagtacagat gaggtaggcg gaattgacgg tgtagcggtg aaatgcttag atatcgtcaa
      181 gaacacctat agcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013531
VERSION     KHUT01013531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggccgac
       61 caagtcaggt gtgaaagcct ggggctcaac cccagaagtg cacttgaaac tggttggctt
      121 gaatgtcgga gagggtagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg actacctgga cgaacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013532
VERSION     KHUT01013532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgagg
       61 aaagttcgat gtgaaagctc cgggctcaac ccggaaatgg cattgaaaac tatcttgctt
      121 gaggattgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caagtcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013533
VERSION     KHUT01013533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           77 g           55 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggaatca ctgggcgtaa aggacgcgta ggcggattat
       61 caagtctttt gtgaaatcta atggcttaac cattaaactg cttgagaaac tgataatcta
      121 gagtgaggga gaggcagatg gaattggtgg tgtaggggta aaatccgtag agatcaccaa
      181 gaatacccat tgcgaaggcg atctgctaga actcaactga cgctaatgcg tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013534
VERSION     KHUT01013534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggtgcg
       61 caagtccgtt gtgaaatccc tgggctcaac ccaggaactg catcggaaac tgcgtgtctt
      121 gagtaatgga gagggaagtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga catttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013535
VERSION     KHUT01013535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctca
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg ccctagaaac ggtgagacta
      121 gagtcttgga gagggttgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcaacctgga caagtactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013536
VERSION     KHUT01013536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          104 g           40 t
ORIGIN      
        1 gacagagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgtgta ggcggggcgg
       61 caagtcgggc gtgaaagcct tcggctcaac cgaagaaggg cgtccgatac tgctgtcctg
      121 gaggattgga gaggagactg gaattcccgg tgtagcggtg aaatgtgtag agatcgggag
      181 gaacaccggt ggcgaaggcg ggtctctggc caattcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013537
VERSION     KHUT01013537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           98 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggaagac
       61 caagtccggg gtgaaagctt tcggctcaac cggaaaatgg ccttggatac tggttttctg
      121 gagggaggta ggggcagctg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccggt ggcgaaagcg agctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013538
VERSION     KHUT01013538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcccga
       61 ccagtcggtg gtgaaagttc gacgcttaac gtcgaatcgg ccaccgatac tgttgggctg
      121 gagcactgta gaggctcatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtgagctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013539
VERSION     KHUT01013539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgca ggcggccatt
       61 caagtcagat gtgaaagccc cgggctcaac ctgggaatag catttgggac tgggtggcta
      121 gagttcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013540
VERSION     KHUT01013540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           94 g           52 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtgag
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaatgg ccttggatac tgcttgtcta
      121 gagggaggta ggggtctgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013541
VERSION     KHUT01013541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatca ctaggcgtaa agcgcgtgta ggcgggtgct
       61 taagtccgtc gtgaaatttc ccggctcaac cgggaaaggt cgacggatac tgggcatctt
      121 gagtatggca ggggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg ggcttctggg ccatcactga cgctaagacg cgaaagttag
      241 gggagcaaac agg
//
LOCUS       KHUT01013542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013542
VERSION     KHUT01013542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttat
       61 taagttggct gtgaaattcc tgggctcaac ctggggtggt cagccaagac tggtagactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013543
VERSION     KHUT01013543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcgggtggt
       61 acagtcagat gtgaaattcc tgggctcaac ctgggggctg catttgatac gtgctgtctt
      121 gagttcggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgaaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013544
VERSION     KHUT01013544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           87 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggtctct
       61 taagtttcat gtgaaatccc tgggctcaac ccaggacgtg catgaaaaac tgggaggctg
      121 gagtgcggta aaagagggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccaga agcgaaggcg gctctctcgg ccgttactga cactaagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013545
VERSION     KHUT01013545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           62 c           99 g           39 t
ORIGIN      
        1 gacgaaccgt gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 caagtccgcg gtgaaatccc ccggctcaac cggggaactg ccgcggatac tggaggtctc
      121 gaggagggta ggggcatgcg gaactacggg tggagcggtg aaatgcgttg atatccgtag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013546
VERSION     KHUT01013546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           93 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggctttt
       61 taagtcagag gtgaaagccc ggggctcaac tccggaactg cctttgagac tgggatgcta
      121 gatcgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgatga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013547
VERSION     KHUT01013547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           86 g           57 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggattta ctgggcgtaa agcgcatgta ggtggcctta
       61 taagtggacg atcaaagacc aacgctcaac gttgggatcg ttgtccatac tgtttggctg
      121 gagggtggca ggggagcatg gaactttgag tgtaggagtg atatccgtag atattcaaag
      181 gaacaccaat ggcgaaggca gtgctctggg ccatttctga cactgagatg cgaaagtgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013548
VERSION     KHUT01013548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           95 g           48 t
ORIGIN      
        1 gacaaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgc
       61 caagtcaggg gtgaaatctt tcggctcaac cggaaaagtg cttctgatac tggcggactt
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013549
VERSION     KHUT01013549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           85 g           50 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggtgtccggt
       61 taagtctccg gtgaaatccc tcggctcaac tgaggaatgg tcggagaaac tagctggatt
      121 gagtgtggca gaggcagacg gaattcccgg tgtagcggta aaatgcgttg atatcgggaa
      181 gaacaccaat ggcgaaggca gtctgctggg ccactactga cactgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013550
VERSION     KHUT01013550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcagaatta ctgggcgtaa agggcgcgta ggcggctcta
       61 taagttagat gtgaaatccc tgggctcaac ctaggaactg catctaagac tgtaggactg
      121 gagtgcagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgacactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013551
VERSION     KHUT01013551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           81 g           60 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattaa ttgggtgtaa agggtgtgca gacggcttat
       61 taagtcactt gttaaattct tcggcctaac tgaaggtctg cgagtgatac tgatgagcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattcctga cgttcataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013552
VERSION     KHUT01013552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatta ctgggcgtaa agagcttgta ggcggcttgc
       61 tcagtccgat gtgaaagccc ccggctcaac cggggaagtg cattggatac tggcgagctt
      121 gagtatggga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga ccaatactga cgctgagaag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013553
VERSION     KHUT01013553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           37 c           90 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggcgcgta ggtgggagat
       61 taagttggat gtgaaagact ggagcttaac ttcgggatgt cattcaatac tgattttcta
      121 gagtacagta gagggtagtg gaatttccag tgtagcggtg aaatgcgtag agattggaaa
      181 gaacaccggt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013554
VERSION     KHUT01013554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 tacgtagggt gcaggcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggtttgt
       61 taagacagat gtgaaatccc cgggcttaac ctgggaactg cgtttgttac tggcagacta
      121 gagtgtggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaataccaat ggcgaaggca gccccctggg ttaacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013555
VERSION     KHUT01013555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           54 c          102 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgtgca ggtggttggt
       61 taaggccgat gtaaaatctc ccggctcacc tgggagggcg cgttggcgac tggccgactg
      121 gaggcaggta gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gccggctggg cctgtcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013556
VERSION     KHUT01013556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c          101 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcaggc
       61 gaggtcgggg gtgaaatccc gcagcttaac tgcggaaggg cctccgatac cggcttgctg
      121 gaggagggta ggggtgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggc ggcgaaagcg gcgcactgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013557
VERSION     KHUT01013557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           90 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtctgt
       61 aaagtcaggt gtgaaagccc cgggctcaac ccgggaagtg cacttgaaac tcacaggcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg gccaactgga cgaatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013558
VERSION     KHUT01013558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           92 g           51 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ttgggcgtaa agcgcacgca ggtggctgtt
       61 caagtctggc gtgaaatctc ttggcttaac caagagcggt cgctggaaac tgaatagctt
      121 gaggcaatga gaggggtgcg gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaacaccagt ggcgaaagcg gcaccctggc attggcctga cactcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013559
VERSION     KHUT01013559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c           98 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggcctga
       61 cacgtcacgc gtgaaatctc ccggctcaac tgggaggggt cgcgtgatac ggtcaggcta
      121 gagcaaggta gggggaagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga cctttgctga cgctaatgcg cgaaagcgtg
      241 ggtagcgatc cgg
//
LOCUS       KHUT01013560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013560
VERSION     KHUT01013560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           55 c           87 g           46 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgcccgg
       61 taagttgggt gtgaaagccc tcggctcaac cgaggaattg cgcccaaaac tgtcgggctc
      121 gaggaagata gaggtgagcg gaactaaggg tggagcggtg aaatgcgttg atatccttag
      181 gaacaccagt agcgaaagcg gctcactggg tcttctctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013561
VERSION     KHUT01013561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           87 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcacgta ggcggcattg
       61 taagttacat ttgaaatttg agggctcaac cttcagaagt ggtgtaagac tgcaaagctt
      121 gaggcaatca aaagaatgtg gaactccgag tgtagcggtg gaatgcgtag agattcggaa
      181 gaacacccat ggcgaaggca gcattctggg attgacctga cgctgaggag cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13721 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013562
VERSION     KHUT01013562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13721"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           62 c          104 g           38 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggactta ctgggcgtaa agcgcgcgca ggcggatggg
       61 tatgtgccgt gtgaaagcgc cgggcttccc ccggcgaggt catggtagac agcccatctt
      121 gagcctcgga gaggtcggtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccagg ggcgaaagcg gccgactggc cgagtgctga cgctgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01013563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013563
VERSION     KHUT01013563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           89 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggagcgta ggcggtttat
       61 taagtgagat gtgaaatacc cgggcttaac ttgggaattg catttcaaac tggttgacta
      121 gagtgcagga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgatgct cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013564
VERSION     KHUT01013564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gctagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggcggcttgt
       61 taagtgaggt gtgaaaccgt gtggctcaac catatcgttg catctcatac tggcaggcta
      121 gaggacggca gaggcaagtg gaattggtag tgtagcagtg aaatgcgtag atattaccag
      181 gaacaccaat ggcgaaggca gcttgctggg ccgaccctga cgctgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013565
VERSION     KHUT01013565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtctga
       61 caagtcttgt gtgaaatctc tgggctcaac ccagagtctg cataggaaac tgtcgggctt
      121 gagtatggga gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt ggcgaaagcg gctcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013566
VERSION     KHUT01013566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           95 g           50 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtttgt
       61 taagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaagac tggcagactg
      121 gagagcagta ggggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttctgga ctgcatctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013567
VERSION     KHUT01013567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           92 g           53 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcaat
       61 caagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaatac tgatggacta
      121 gagtgcggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013568
VERSION     KHUT01013568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttaat
       61 taagtcgtgt gtgaaatccc tgggcttaac ctaggaattg catatgaaac tgattagcta
      121 gagtttggga gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actccctggc ccgatactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013569
VERSION     KHUT01013569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           86 g           55 t
ORIGIN      
        1 tacgtagatg gcgagcgtta tccggaatga ctgggcgtaa agggtgcgta ggcggttatg
       61 taagtcagat gtgaaaatcc cgagcttaac ttgggaactg catttgaaac tatgtgactt
      121 gagtacagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013570
VERSION     KHUT01013570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          104 g           43 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttcgc
       61 tccgtccggt gtgaaatctc ccggctcacc tgggaggggg cgctggagac tggcgaactg
      121 gaggcaggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gccgactgga cctgtcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013571
VERSION     KHUT01013571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c           99 g           43 t
ORIGIN      
        1 gacgtaggga gcgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcctcg
       61 caagtcggtt gtgaaatccc ggggctcaac cccgggactg cgtccgatac tgcgaggctg
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gagcaccggt ggcgaaggcg gatctctggg ccttacctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013572
VERSION     KHUT01013572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           91 g           47 t
ORIGIN      
        1 gacgtagggg gcaagtgttc tccggaatca ttgggcgtaa agcgcatgca ggcggtcaat
       61 caagttcatg ctgacaattc tcggcttaac cgggaagggt gtgtgaagac tggttgactt
      121 gagagcgaga gagggacacg gaattccagg tggagcagtg aaatgcgtag agatctggag
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtttctga cgctcagatg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013573
VERSION     KHUT01013573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ctgggcgtaa agagttcgta ggcggtttgt
       61 taagtcgaat gtgaaagccc tgggctcaat ccaggaagtg cattcgatac tggcaagcta
      121 gagtgcagga gaggaaggcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gccttctgga ctgtcactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013574
VERSION     KHUT01013574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           90 g           47 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtccgca ggtggctgtg
       61 taagtctgct gttaaagagt catgctcaac atgataagag cagtggaaac tacacggcta
      121 gagtgcgttc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agatcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctagg ccgcaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01013575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013575
VERSION     KHUT01013575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           83 g           54 t
ORIGIN      
        1 gacgaggggt gcaaacgtta ttcggaatga ttgggcgtaa agggtgcgta ggcggtttat
       61 caagtcaact gttaaatttc tcagcttaac tgagagtatg cggtagaaac tgacagacta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttagt ccatttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013576
VERSION     KHUT01013576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           91 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggagcgta ggcggtcttt
       61 taagtgggat gtgaaatacc cgggctcaac ctgggaattg cattccaaac tgggagacta
      121 gagtgcagga gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag agatttggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtaactga cgctgaggct cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013577
VERSION     KHUT01013577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           96 g           49 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcaatt
       61 taagtctgat gtgaaagccc ggggcttacc cccggaagtg cattggatac tgggttgctg
      121 gagtactgga gaggagggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gccctctgga cagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013578
VERSION     KHUT01013578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           95 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa aaggttcgta ggtggctcgt
       61 ccagtcgagc gtgaaatctc ccggcttaac tgggagaggt cgttcgatac tagcgggctc
      121 gaggtcggta gagggaagcg gaattccggg tgtagtggtg gaatacgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgattctga cactgaggaa cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01013579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013579
VERSION     KHUT01013579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggttata
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaatgg cattgaaaac tgtaggactt
      121 gagtcggtga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacatcagt ggcgaaggcg tctgcctggc accagactga cacttaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013580
VERSION     KHUT01013580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           54 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtcggat gtgaaatcct acagcttaac tgtagaactg cattcgaaac tgctcagcta
      121 gagtactgga gaggagatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gatctctgga cagttactga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013581
VERSION     KHUT01013581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctctt gtgaaagctc ccggctcaac tgggagaggt caagggaaac taccgggcta
      121 gagtgtggca gaggagactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtctctggg ccgtcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013582
VERSION     KHUT01013582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           99 g           37 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctcgg
       61 caagtcggga gtgaaagccc ggggctcaac cccggaacgg ctctcgaaac tgccgggctc
      121 gagtgcggaa gaggcaaccg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacacctga ggcgaaggcg ggttgctggg ccgacactga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013583
VERSION     KHUT01013583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           79 g           57 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggttatg
       61 taagttagat gtgaaagccc tgggcttaac ctgggaactg catttaatac tgcacaactc
      121 gagtgcgata gaggagagcg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaataccgat ggcgaaggca gctctctggc tcgcaactga cactgagatt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013584
VERSION     KHUT01013584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           57 c           90 g           46 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccttc
       61 caagtcaggt gtgaaatccc ccggcccaac cggggaactg cgcttgaaac tggttggctt
      121 gagtcagcta gaggtgcacg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacaccaga ggcgaaagcg gtgcactgga gctggtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013585
VERSION     KHUT01013585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggacttt
       61 taagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgatac tgggagtctc
      121 gagttcggaa gaggttggtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gccaactggt ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013586
VERSION     KHUT01013586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           94 g           48 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggtggtttga
       61 taagtcactg ttgaaagctc ccggctaaac tgggagaggt gcggtgagac tgtcaggcta
      121 gagtatggga gagggatgca gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaataccaga ggcgaaggcg gcatcctggc ctgttactga cactgagacg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13746 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013587
VERSION     KHUT01013587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13746"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           95 g           42 t
ORIGIN      
        1 tacggagggg gttagcgttg ttcggaatga ctgggcgtaa agcgcgcgta ggcgggctgg
       61 aaagtcagag gtgaaagccc ggggctcaac cccggaattg cctttgaaac tcccagcctc
      121 gagttcgaga gaggtgggtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gcccactggc tcgacactga cgctgaggcg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01013588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013588
VERSION     KHUT01013588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           93 g           49 t
ORIGIN      
        1 gacggaggtc ccgagcgttg ttcggaatta ttgggcttaa agcgcgtgta ggcgggatgg
       61 caagtacctt gtgaaagccc cggactcaat ccgggaactg ctcggtatac tgccgttctc
      121 gaggcaggta ggggtcactg gaaccttagg tggagcggtg aaatgcgtag atatctaagg
      181 gaacgcctgt ggcgaaagcg ggtgactggg cctgtcctga cgctgagacg cgaaagcgta
      241 gggagcaaac ggg
//
LOCUS       KHUT01013589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013589
VERSION     KHUT01013589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           57 c          107 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaacgt
       61 taagcgtggc ctgaaagccc ccggctcaac cggggaaggt ggctgcgtac tggcgttctt
      121 gagggcgaca gaggcgggtg gaattccggg tgtagcggtg aaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gcccgctggg tcggctctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013590
VERSION     KHUT01013590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           84 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttgag
       61 taagtctgat gtgaaatctc cgagctcaac tcggaaatgg cattggaaac tacttgacta
      121 gagagttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caacttctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013591
VERSION     KHUT01013591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           90 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttagt
       61 taagttatct gtgaaatacc tgggctcaac ccaggaaggt cagataagac tgattgacta
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctgcctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013592
VERSION     KHUT01013592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           98 g           45 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccgct
       61 caagtcggat gtgaaatccc tcggcttaac cgaggaactg cattcgatac tgggtggcta
      121 gagtccggga aggggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctacctgga ccggtactga cgctgaggcg cgaaagccag
      241 gggagcgaat ggg
//
LOCUS       KHUT01013593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013593
VERSION     KHUT01013593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           95 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacggt
       61 caagtcggtg gtgaaatcct tcagcttaac tggagaatgg ccgccgatac tgattgtctc
      121 gagggaggta ggggcatgtg gaactgaggg tggagcggtg aaatgcgtag atatcctcag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013594
VERSION     KHUT01013594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           86 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgtta ttcggattaa ctgggcgtaa agagcgtgtc ggcggtcttt
       61 taagtcaact gtaaaagagc tcggcttaac cgagtaaatg cgggtgatac tggaagacta
      121 gagtacggga gagaagggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccctttggc ccgtaactga cgcttaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013595
VERSION     KHUT01013595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           92 g           43 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agagcgcgta ggcggttacc
       61 taagtcggat gtgaaagccc tcggcttaac cgaggaagtg cacccgaaac tgagtagctg
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgagactga cgctaaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013596
VERSION     KHUT01013596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           77 g           57 t
ORIGIN      
        1 gacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agcgttcgta ggtggtttgt
       61 taagtaggat gttaaatccc tgagctcaac tcaggattgg tatcctaaac tggcaagcta
      121 gagacaggta gaggcagatg gaattctgta tgtaggggta atatccgtag atatacagag
      181 gaacaccaaa agcgaaggca gtctgctgga cctgttctga cactcaagaa cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013597
VERSION     KHUT01013597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           84 g           50 t
ORIGIN      
        1 gacgtaccac ccgaacgtta ttcggattta ctgggcttaa agagtccgta ggcggccaag
       61 taagttggat gtgaaatacc tcggcttaac cgaggagctg cattcaaaac tacttggctc
      121 gagagagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggga cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013598
VERSION     KHUT01013598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccgcg
       61 caagtttgat gtgaaatctc cgggcttaac ccggaaatgg cattgaatac tgcgtggctg
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013599
VERSION     KHUT01013599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           82 g           49 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggctaag
       61 tgtgtggagt gtgaaagctc gaagcttaac ttcgaaactg caccccaaac tacttagcta
      121 gagcattgga gagagtagca gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaataccaga ggcgaaggcg gctacttgga caattgctga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01013600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013600
VERSION     KHUT01013600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgtgg
       61 aaagtcaggt gtgaaatccg gaggctcaac ctccgaattg catccgatac ttccatgcta
      121 gagagttgta gaggggcttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagaa
      181 gaacgctcgc agcgaaggcg aagctctggg caacatctga cgctgaggca cgaaggctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013601
VERSION     KHUT01013601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           87 g           50 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgcag
       61 taagttgggt gtgaaatccc tcggcttaac cgaggaactg cgcccaaaac tactgtgctt
      121 gagggagata gaggtgagcg gaactgaggg tggagcggtg aaatgcgttg atatcctcag
      181 gaacacccgt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013602
VERSION     KHUT01013602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           89 g           48 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agcgcgcgta ggcgggcatt
       61 caagtcggtc gtgaaatccc ccagctcaac tggggaaccg cgtccgatac tgtctgtctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaggcg actctctggc tcgtatctga cgctgaggcg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013603
VERSION     KHUT01013603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           83 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaaag
       61 aaagttagat gtgaaagcct gaggcttaac ttcagaacag catctaaaac tccttttcta
      121 gaggatgagc ggggaaaatg gaattccaca tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccggt ggcgaaggcg attttctagc ttagacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013604
VERSION     KHUT01013604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           94 g           43 t
ORIGIN      
        1 tacaggaggc ccgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcgggccag
       61 taagtatctc gtgaaatacc tcggctcaac cgaggaatgg cggggtaaac tactggtctt
      121 gaggtaggta ggggttgctg gaacgctcgg tggagcggtg aaatgcgtag agatcgagcg
      181 gaacgccgaa ggtgaagaca agcaactggg cctatcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013605
VERSION     KHUT01013605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggtttgc
       61 caagtagggg gtgaaatccc ggagcttaac tccggaattg ccttctagac tggcagactt
      121 gagaccgaga gaggtaaggg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg ccttactggc tcggcactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013606
VERSION     KHUT01013606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggctcag
       61 caagtccgct ctgaaagccc agggctcaac cctgggatgc gggtggatac tgctgagctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013607
VERSION     KHUT01013607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           79 g           60 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatca ttgggcgtaa agcgagtgcg ggtggctttt
       61 taagtcagga gtgaaatccc agagcttaac tctggaagtg cttttgatac tggaaagctt
      121 gaatgtggca gaggatacta gaatacctaa tgtagtggtg aaatacgtag atattaggtg
      181 gaacaccggt ggcgaaggcg ggtatctggg ccaacattga cacttagact cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013608
VERSION     KHUT01013608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           87 g           53 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggattca ctgggtgtaa agggtgcgca ggcgggatag
       61 taagtcagcg gtgaaatctc acggctcaac cgtgaaactg ccgttgatac tgctgttctt
      121 gagttcggga gaggttgatg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gtcaactggc ccgtaactga cgctcatgca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013609
VERSION     KHUT01013609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggtggcg
       61 taagtcggat gtgaaatccc tcggcttaac cgaggacgtg cacccgatac tgtgccactt
      121 gagtaccgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggtaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013610
VERSION     KHUT01013610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           82 g           57 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattaa ttgggcgtaa agggtgcgca gacggtttgt
       61 taagtcactt gttaaattct tcggcctaac tgaagatctg cgagtgatac tggcgaacta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccacatctga cgttcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013611
VERSION     KHUT01013611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           91 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcaggccgc
       61 caagtcgggg gtgaaatttt tcggcttaac cggaaaacag ctttcgatac tggcagtctc
      121 gagggaggta ggggcagatg gaacttgtgg tggagcggtg aaatgcgttg atatcacaag
      181 gaactccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013612
VERSION     KHUT01013612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           83 g           54 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatta ttgggcgtaa agggtgtgta gacggcatgt
       61 taagtcaatt gttaaattct ccggcctaac cggaggcaag cagtagaaac tggcgtgcta
      121 gagggtggaa gagagaagcg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatttctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013613
VERSION     KHUT01013613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           88 g           51 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggtggtcttc
       61 caagttggat gtgaaagcct tgggcttaac ccaagaaatg cattcaggac tggaagacta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cactaagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013614
VERSION     KHUT01013614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtccta
       61 taagtcaggt gtgaaagccc ggggctcaac cccggaagtg cacttgatac tgttggacta
      121 gagtaccgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cggatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013615
VERSION     KHUT01013615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           90 g           44 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggtctga
       61 taagtcggac gtgaaatccc ggggctcaac cccggaagag cgctcgatac tgtctgactt
      121 gggttctgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagataccga cgctgagacg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01013616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013616
VERSION     KHUT01013616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           82 g           49 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtacgca ggcggccatg
       61 caagtcagaa gtgaaagccc tgggctcaac ccaggaatag cttttgaaac tgcatggcta
      121 gaatatggga gaggttggcg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccagt ggcgaaggcg gccaactgga ccattattga cgctcatgta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013617
VERSION     KHUT01013617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           83 g           55 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtcagta ggcggtcgag
       61 ttagtcttct gtcaaattct tcggcttaac cgggggtccg cagaggaaac ggctcgactt
      121 gaggatgcga gaggtgaatg gaactcatag tgtaggggtg aaatccgttg atattatggg
      181 gaacaccaaa agcgaaggca attcactggc gcactcctga cgctgaatga cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013618
VERSION     KHUT01013618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           97 g           43 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agggcacgca ggtggtcgtg
       61 caagtgccgt gtgaaagcag ctagcttaac tggctgaggt catggcagac tgcgcgactc
      121 gagcccgtga gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccat ggagaaatca gcggactggc acggtgctga cactgaggtg cgaaagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01013619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013619
VERSION     KHUT01013619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           87 g           56 t
ORIGIN      
        1 tacgtaggga ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttttg
       61 taagtcggga gttaaaacat caggctcaac ctggtgtcgc ttttgatact gcaatgactt
      121 gagtgtggca ggggaagaca gaattccacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaataccgat ggcgaaggca gtcttctggg ccaatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013620
VERSION     KHUT01013620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           85 g           55 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtgcgta ggcggttacg
       61 taagtctgat attaaaaacc ggagctcaac tccggggctg tatcggaaac tgtgtaacta
      121 gaggacaata gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg gacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013621
VERSION     KHUT01013621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           93 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtcggct gtgaaatccc gaggctcaac ctcgggcttg cagtcgatac gggcagacta
      121 gagtggagca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca ggtctctggg ctttaactga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013622
VERSION     KHUT01013622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctcgg
       61 taagtcggct gtgaaagccc tcggctcaac cgaggaattg cgaccgatac tgccgggctt
      121 gagtcccgga gagggtagcg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccagt ggcgaaggcg gctacctgga cgggtactga cgctgatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013623
VERSION     KHUT01013623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           60 c          109 g           35 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgg
       61 taagggggcg gtgaaagccc ggggctcaac cccgggtctg ccggaccgac tgccgagctt
      121 gagcacacta ggggcaggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgccggg gtgtggctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013624
VERSION     KHUT01013624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           43 c           95 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggcgtaa agggcgtgta ggtgggccat
       61 taagtcttgt gtctaagtgc ggtgcttaac gccgtatagg cgcaggaaac tgaaggtctt
      121 gagtgcagga gaggaaagtg gaattcccgg agtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctgga ctgtgtctga cactgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013625
VERSION     KHUT01013625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           83 g           53 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgcgta ggtggcgtgt
       61 taagtgagat gttaaaccgt gtggctcaac catatcgttg catctcatac tggcatgcta
      121 gaggacggca gaggcaagtg gaattggtag tgtagcagtg aaatgcgtag atattaccag
      181 gaacaccaat ggcgaaggca gcttgctggg ccactcctga cactgaggta cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013626
VERSION     KHUT01013626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggtggtttaa
       61 taagttatct gtgaaaaccc tgggctcaac ctggggctga gagataagac tgtaagacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccggt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013627
VERSION     KHUT01013627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           94 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatggt
       61 acagtcagat gtgaaattcc cgggcttaac ctgggggctg catttgatac gtattgtctt
      121 gagttcggaa gagggtcgtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013628
VERSION     KHUT01013628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtctta
       61 taagttagtt gtgaaatacc tgggcttaac ctgggaactg caactaagac tgtgagactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013629
VERSION     KHUT01013629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtccgac gtgaaaggct tcggctcaac cgaagaaggc cgttggaaac tgccgttctt
      121 gagcgtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt cggcgactga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013630
VERSION     KHUT01013630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggccatt
       61 caagtcaggg gtgaaagccc agagctcaac tctggaactg cccttgaaac tgtatggctt
      121 gaggatgaga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc tcatttctga cgctcatgca cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013631
VERSION     KHUT01013631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           50 c           83 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta gtcggaatta ctgggcgtaa agggtgcgta ggcggatatg
       61 caagtcagac gtgaaattcc gaggctcaac ctcggagctg catctgaaac tgcataacta
      121 gcggaatggc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ctactccgga cgctgaggca cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013632
VERSION     KHUT01013632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           86 g           53 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggctatt
       61 caagtcagtt gttaaagcgt ggggcttaac tccataaagg caaatgaaac tgattagcta
      121 gagtgcgata ggggcaaggg gaatttccag tgtagcggtg aaatgcgtag atattggaaa
      181 gaacaccggt ggcgaaagcg ccttgctggg tctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01013633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013633
VERSION     KHUT01013633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           95 g           48 t
ORIGIN      
        1 cacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggtggtccgc
       61 tgcgtccgct gtgaaaatcc aaggcttaac cttgggcttg cagtggatac gggcagacta
      121 gaggtaggta ggggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gttctctggg ccttacctga cactgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013634
VERSION     KHUT01013634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           94 g           48 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgc
       61 caagtcgggg gtgaaatcct gcagctcaac tgtagaagtg ccttcgatac tggcagtctg
      121 gagggaggta ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccgga ggcgaaagcg gcgcactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013635
VERSION     KHUT01013635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggatcgt
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tggcgatctt
      121 gggccactca ggggttgccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgaa ggtgaagaca ggcaactggg agtgcgccga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13795 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013636
VERSION     KHUT01013636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13795"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcga
       61 tgagtcaacg gtgaaagctc gacgctcaac gtcgagtcgg ccgtcgatac tgtcgcgctc
      121 gagcactgta gaggccaatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gttggctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013637
VERSION     KHUT01013637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           50 c           94 g           52 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatcat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tggtggtctt
      121 gagggaggta ggggcatgtg gaactccagg tggagcggtg aaatgcgttg atatctggag
      181 gaactccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013638
VERSION     KHUT01013638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           52 c           85 g           47 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agggtgcgca ggcggccaag
       61 tgtgtcaggt gtgaaatccc gaggcttaac ctcggaactg cgcctgaaac tacttggcta
      121 gagtactgga gagggtaacg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaga ggcgaaggcg gttacctgga cagttactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013639
VERSION     KHUT01013639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           92 g           52 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggtggtttgt
       61 taagtcacgg gtgaaagttt tcggcccaac cggaaaattg cccgtgatac tggcgaactt
      121 gagtgcgtga ggggttaccg gaacgagcag tgtagcggtg aaatgcgtag atattgctcg
      181 gaaggccaga ggcgaaggcg ggtaactggt gcgcaactga cactgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013640
VERSION     KHUT01013640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           95 g           41 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tcccgcgctt
      121 gagggctgga gaggagtccg gaattcacgg tggagcagtg aaatgcgtgg atatcgtgag
      181 gaacactagc ggcgaaggcg ggactctgga cagaacctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013641
VERSION     KHUT01013641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgta ggcggctggc
       61 taagttggat gtgaaatctc ccggctcaac tgggagagga cgttcaagac tggccggcta
      121 gaggacggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg accttctgga ccgttcctga cgctgagatg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013642
VERSION     KHUT01013642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           87 g           49 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttact
       61 taagtcaggg gtgaaatccc ggggctcaac cccggaactg cccttgaaac tgggcaacta
      121 gaatcttgga gaggtcagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actgactgga caagtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013643             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013643
VERSION     KHUT01013643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           84 g           51 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcacgta ggcggcttgt
       61 taagtgtgaa gtgaaatctc cagagctcaa ctcggaaacc gcttcgcata ctggcaagct
      121 agaggaatgc agaggtaagt ggaattcctg gtgtagcggt gaaatgcata gatatcagga
      181 ggaacaccca tggcgaaggc agcttactgg gcattatctg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013644
VERSION     KHUT01013644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           85 g           53 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggtaggta ggtggtcata
       61 aaagttcggt gtgaaagccc acagctcaac tgtggaactg cattgaatac tctatgactt
      121 gagtactgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cggtaactga cactgagcta cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013645
VERSION     KHUT01013645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           79 g           61 t
ORIGIN      
        1 tacgagtggt gctagcgtta ttcggattta ttgggcgtaa agggtgtgta gacggcttct
       61 taagtcactt gtttaattct tcggcctaac cgaagctcag cgagtgaaac tgattagctt
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatccctga cgttgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013646
VERSION     KHUT01013646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           82 g           55 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggtgtaa agggtgctca ggtggtctga
       61 taagtcagag gtgaaagccc agggctcaac cctggaactg cctttgatac tatcaggctt
      121 gagtatggaa gaggttgatg gaatttctgg tgtagcagtg aaatgcgtag atatcagaaa
      181 gaacaccagt ggcgaaggcg gtcaactggt ccattactga cactaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013647
VERSION     KHUT01013647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c           96 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgga
       61 ttagtcgcgt gtgaaagccc ccggcttaac tggggagggt cacgcgatac tgttcgactt
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013648
VERSION     KHUT01013648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           84 g           56 t
ORIGIN      
        1 tacggggggt ccaagcgttg ttcggaatca ctgggcgtaa agcgcatgta ggtggctttt
       61 caagtctgtt gtgaaagccc agggcctaac cctggaagtg cagcagatac tgttaagctt
      121 gagtatggga gaggatagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gctatctggc ccaatactga cactaagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013649
VERSION     KHUT01013649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           87 g           53 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggattta ctgggcgtaa agagtgtgta gacggtttgc
       61 caagtctgct gttaaattcc tcggcttaac cgagggtcag cagtagaaac tggcggacta
      121 gagtgtggga gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccgat ggcgaaggca gcttcttgga ccattactga cgttgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013650
VERSION     KHUT01013650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           89 g           45 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtccaa
       61 taagtccgct gtgaaagtcg aaaactcaac cctcgaatgc cggtggatac tgtcgggcta
      121 gagtgcggaa gagacgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013651
VERSION     KHUT01013651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           49 t
ORIGIN      
        1 gacgaacctt gctaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcggag
       61 aaagttgggt gtgaaatccc tcagctcaac tgaggaactg cgcccaaaac tacttcgctc
      121 gaggaagata ggggtacgcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gcgtactggg tcttctctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013652
VERSION     KHUT01013652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           59 c          111 g           36 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agggcgcgca ggcggtccgc
       61 tgcatcggtg gtgaaagccc cgggcttaac ccgggagggt ccatcgagat gagcggactg
      121 gaggcagggc gagggcggtg gaattccggg tgtagtggtg aaatgcgtag agctccggag
      181 gaacaccagt ggcgaaggcg gccgcctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013653
VERSION     KHUT01013653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           79 g           61 t
ORIGIN      
        1 tacgtagggt gctagagttg tccggattta ctgggtgtaa agggtgctca ggcggatttg
       61 taagtcagag gtgaaatccc aaagcttaac tttggagctg cctttgatac tgcaagtctt
      121 gagtgtgaga gaggatgatg gaatatctgg tgtagcagtg aaatgcgtag atatcagata
      181 gaacaccaat ggcgaaggca gtcatctggc tcaatactga cgctaaagca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013654
VERSION     KHUT01013654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 cacgtatggg gcgagcgttg ttcggaatta ttgggcgtaa agggtgtgca ggcggttatg
       61 taagtctgac gtgaaagact cgagcttaac tcgaggaacg cgttggaaac tgtgtgacta
      121 gagttctgga ggggtagctg gaattccagg tgtaggggtg aaatctgtag atatctggaa
      181 gaacatcagt ggcgaaggcg agctactagc caaggactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013655
VERSION     KHUT01013655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          102 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtctct
       61 taagtcggct gtgaaatctc tgggcccaac ccagaggggt cggccgagac tgggggactc
      121 gagggtggta gaggaaggtg gaataccggg tgtagtggtg aaatgcgtag agatccggtg
      181 gaacaccagt ggcgaaggcg gccttctggg ccatacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cga
//
LOCUS       KHUT01013656             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013656
VERSION     KHUT01013656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           54 t
ORIGIN      
        1 tacagaggtc tcgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtttgg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgctttaact
      121 tgagtgctgg agaggagatc ggaatttacg gtgtagcagt gaaatgcgta gatatcgtaa
      181 ggaagaccag tggcgaaggc ggatctctgg acagcaactg acactgaggc acgaaggcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013657
VERSION     KHUT01013657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           74 g           65 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggtcttg
       61 taagtcagcg gtgaaagcct acagcttaac tgtagaattg ccattgatac tgcttgactt
      121 gaatcatgtc gaggtggatg gaatatatca tgtagcggtg aaatgcttag atatgatata
      181 gaacaccaat tgcgaaggca gttcactaga catgtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013658
VERSION     KHUT01013658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggatgtt
       61 caagtcagag gtgaaagccc tgggctcaac ccgggaattg cctttgaaac tggacgtctt
      121 gagtatggga gagggtggcg gaatacccag tgtagaggtg aaattcgtag atattgggta
      181 gaacaccagt ggcgaaggcg gccacctggc ccataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013659
VERSION     KHUT01013659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           95 g           42 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcagg
       61 caagtggaat gtgaaatgcc ccggctcacc cggggaccgg catcccaaac tgcttgactt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013660
VERSION     KHUT01013660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcgaaatta ctgggcgtaa agcgcgtgta ggcggtctga
       61 taagttggac gtgaaatccc tcggcttaac cgaggaactg cgttcaaaac tgccagacta
      121 gagtatcgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg accatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013661
VERSION     KHUT01013661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcgtaa agagcgcgta ggtggtttgg
       61 taagtctgat gtgaaagccc tggactcaat tcaggaagcg cattggatac tgccagacta
      121 gagtgcagga gaggaaagcg gaattccagg agtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctttctgga ctgcaactga cactaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013662
VERSION     KHUT01013662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 tacgtaggat ccgagcgttg tccggattta ctgggcgtaa agggtgtgta ggcggcttgc
       61 taagtcttgt gtgaaatctc ccggcccaac tgggaggggt catgggaaac tagcaggcta
      121 gagggcagta gaggagagcg gaattccggg agtagtggtg aaatacgtag atacccggag
      181 gaacaccagt ggcgaaagcg gctctctggg ctgtccctga cgctgaggca cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01013663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013663
VERSION     KHUT01013663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggtctgg
       61 caagtcgggc atgaaatctc ttggctcaac cgggagaggt tgtccgatac ttccagactt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccacctgga ctgcacctga cgctaaaacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013664
VERSION     KHUT01013664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           81 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcctta
       61 taagtcagtg gtgaaagcct gcagcttaac tgtaggactg ccattgatac tgcaaggctt
      121 gagtactgat gaggtaggcg gaattgatag tgtagcggtg aaatgcatag atattatcaa
      181 gaacaccgat tgcgaaggca gcttactaag gagttactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013665
VERSION     KHUT01013665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           87 g           56 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatta ctgggtttaa agggtgcgta ggcggcttag
       61 taagtcagag gtgaaaggct acggcttaac cgtggaattg cctatgatac tgctgagctt
      121 gaattgggtt gaggtaagcg gaatgtggca tgtagcggtg aaatgcatag atatgccata
      181 gaacaccgat tgcgaaggca gcttactgga cctggattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013666             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013666
VERSION     KHUT01013666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           66 c          103 g           32 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggctcg
       61 cgcgtcggac gctgaaagcc cccggctcaa ccggggaacg ggcgccgata cggcgggcct
      121 ggaggggggt agggggcgct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg cggcgaaagc gagcgcctgg acccttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013667
VERSION     KHUT01013667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           81 g           62 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcgggctgg
       61 taagtcagtg gtgaaataca cgtgctcaac atgtgaactg ccattgatac tgccggtctt
      121 gaatatgagt gaggtaggcg gaatgttgca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccaat tgcgtaggca gcttactaac tcatgattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013668
VERSION     KHUT01013668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgttg ttcggaatta ctgggcgtaa agagcttgta ggtggctctg
       61 taagttagat gtgaaagcct ggggctcaac cccagaagtg catctaaaac tgcagagctt
      121 gagtatggga gagggtgacg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gttgcctggc ccaatactga cactgagaag cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013669
VERSION     KHUT01013669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           97 g           44 t
ORIGIN      
        1 tacggaaggg gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggccgct
       61 caagtgaggc gtgaaagccc cgggcttaac ctgggaaccg cgcttcagac tgggtggctc
      121 gagtccggga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccacctggc ccggtactga cgctatagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013670             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013670
VERSION     KHUT01013670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gccagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggcgcag
       61 tgtgtttggt gtgaaatctc ccggctcaac cgggaggggc catcgaaaac tgctgtgctt
      121 cgggtttggg agaggggagt ggaattcccg gtgtagcggt gaaatgcgta gagatcggga
      181 ggaacaccag cgacgaaggt ggctccctgg accaataccg acgctgaggc gcgaaagcta
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013671
VERSION     KHUT01013671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           88 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 gaagttcgat gtgaaatccc cgggctcaac ccgggaactg cattgaaaac tctctcgctt
      121 gagggtcgga ggggagactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013672
VERSION     KHUT01013672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggttggt
       61 taagtctgtc gtgaaagccc tgggctcaac ctgggaatgg cgatggatac tggctagcta
      121 gagtgtaata gaggatggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacatccgt ggcgaaggcg gccatctgga tcaacactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013673
VERSION     KHUT01013673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           88 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctttt gtgaaagctc ccggctcaac tgggagaggt caagggaaac taccgggctg
      121 gagtgtggca gaggaagcta gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccattactga cgctaagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013674
VERSION     KHUT01013674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggttaaa
       61 caagtcacat gtgaaatacc agggctcaac cttggggggt catgtgaaac tgtataactt
      121 gagtgcagta gaggagagcg gaattcctgg tgtagcggta aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gctctctggg ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013675
VERSION     KHUT01013675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           41 c           86 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggtggtcatt
       61 taagtctgat ttgaaagctg gtggcttaac catcagatgt ggttggaaac tgagtgactt
      121 gaatgggttg gcggtagccg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaacaccgat tgcgaaggca ggctactacg acttgattga cactgaggca cgagagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013676
VERSION     KHUT01013676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           91 g           54 t
ORIGIN      
        1 tacagggggt gcgagcgtta ttcggattta ttgggcgtaa agagcgtgta ggcggtttgc
       61 taagtctgat gtgaaagccc tgggctcaat ccaggaagtg cattggatac tggcagacta
      121 gagtacagga ggggtaagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gcttactgga ctgttactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013677
VERSION     KHUT01013677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           88 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa aggatgcgta ggcggaatct
       61 taagtcagat gtgaaatccc cgggcttaac ccgggaatag catttgaaac tggggttcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013678
VERSION     KHUT01013678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttact
       61 taagtcttat gtgaaatccc tgggcttaac ctaggaattg cataggaaac tgagtagcta
      121 gagtacggaa gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg acttcctggt ccgttactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013679
VERSION     KHUT01013679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcggcacgg
       61 acagtcaggc gtgaaattcc tgggcttaac ctaggggctg cgcttgatac gtccgagcta
      121 gaggatggaa gaggctcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccattactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013680
VERSION     KHUT01013680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatgtt
       61 taagtgagat gtgaaatacc caggctcaac ctgggagctg catttcaaac tggatatcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca tgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013681
VERSION     KHUT01013681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           88 g           45 t
ORIGIN      
        1 gacagaggat gcaagcgtta tccggaatga ttgggcgtaa agcgtctgta ggtggctttt
       61 taagtctgcc gttaaatccc agggcttaac cctggataag cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaatctccgg tggagcggtg aaatgcgtag agatcggaaa
      181 gaacaccaac ggcgaaggca ctctgctggg ccgacactga cactgagaga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01013682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013682
VERSION     KHUT01013682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           89 g           45 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcgcgg
       61 aaagttgggt gtgaaatccc tcggctcaac cgaggaatgg cgcccaaaac taccgtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgatgca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013683
VERSION     KHUT01013683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           89 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtgatg
       61 caagtcaggg gtgaaatccc ggagcttaac tccggaactg cctttgaaac tgcattgctg
      121 gagtgtgtga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctggc acacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013684
VERSION     KHUT01013684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           85 a           30 c           67 g           71 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggaatta ttgggcgtaa agggtttgta ggtggatatt
       61 taagtctttt gttaaatctt taagcttaac ttaaaatatg caaaagaaac tgtttatctt
      121 gagtatgcta aaggtaaagg gaattttcag tggagcggtg aaatgcgtag atattgaaag
      181 gaacaccgaa ggcgaaagca ctttacttgg tcattactga cactgaaaaa cgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01013685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013685
VERSION     KHUT01013685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           65 c          100 g           36 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggctcgg
       61 cgcgtggggt gtgaaactgg ccggctcaac cggtcatcgc cacctcatac ggctgggctc
      121 gagcccgtga gaggtccgtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gcggactggc acgcggctga cgctcaggtg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013686
VERSION     KHUT01013686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           86 g           45 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 taagtggggt gtgaaagccc tcggctcaac cgaggaactg cgctccaaac taccgtgctt
      121 gagtgagaca gaggtaagcg gaacttcggg tggaacggtg aaatgtgttg atatccgaag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcaactga cgctgatgca cgaaagccag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013687
VERSION     KHUT01013687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           76 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggtttt
       61 taagtctggg gtgaaatccc gaagctcaac ttcggaactg ccctggatac tattagcctt
      121 gaatcttgtt gaggttggcg gaatataaca tgtagcggtg aaatgcatag atatgttata
      181 gaacaccgat tgcgaaggca gctaactaaa caagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013688
VERSION     KHUT01013688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggttgcc
       61 taagtctggt gttaaatact agggctcaac cctggcatgg cattggaaac tgggcgacta
      121 gagtgcggta gaggtatcgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccagt ggcgaaagcg cgatactggg ccgtaactga cgctgaggaa cgaaagccag
      241 ggtagcaaaa ggg
//
LOCUS       KHUT01013689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013689
VERSION     KHUT01013689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           85 g           53 t
ORIGIN      
        1 tacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgtt gtgtaagctc ctggcttaac tgggagaggt caacggaaac tatcagactt
      121 gagtatggga gaggatactg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca ggtatctgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013690
VERSION     KHUT01013690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           89 g           45 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtctta
       61 caagacaggt gtgaaatccc cgggctcaac ctgggaactg cacttgtgac tgtaaggctg
      121 gagtacggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgtactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013691
VERSION     KHUT01013691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           44 c           86 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggtttgt
       61 taagatggat gtgaaatccc caagctcaac ttgggaatgg catacatgac tggcaagcta
      121 gaatatggta gagggaggtg gaattccatg tgtagcagtg aaatgcgtag agatatggag
      181 gaacaccgat ggcgaaggca gcctcctgga ccaatattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013692
VERSION     KHUT01013692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggcaagta ggtggtctta
       61 tttgtctagg gtgaaatcct tgagcttaac tcaagaagtg ccctagaaac ggtgagacta
      121 gagttctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagacactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013693
VERSION     KHUT01013693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           83 g           54 t
ORIGIN      
        1 tacgaaggtg gctagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtttga
       61 taagtcaggt gtgaaatccc acagcttaac tgtggacacg cgcccgatac tgtcagacta
      121 gagtatgaga ggggtaagag gaatttccag tgtaggggtg aaatccgtag atattggaaa
      181 gaacaccggc cgcgaaggcg tcttactggc tcattactga cgctcaggga cgaaagccaa
      241 gggagcaaac ggg
//
LOCUS       KHUT01013694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013694
VERSION     KHUT01013694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           45 c           97 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatga ctgggcgtaa agcgtgcgta ggtggttgtg
       61 taagacaggc gtgaaatgcc cgggcttaac ctgggacgtg cgcttgtgac tgcatggcta
      121 gaatgcatca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg gtgtgattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013695
VERSION     KHUT01013695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtctag
       61 tcagtctagt gtgaaatccc caggcttaac ttgggaactg cgctggatac tgctggacta
      121 gagtgctaga ggggttcgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaga ggcgaaggcg gcgaactgga tagcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013696
VERSION     KHUT01013696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgt
       61 caagtcgggg gtgaaatcca gcagcttaac tgttgaagtg cctccgatac tgtcaagctc
      121 gaggaaggta ggggtgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg gcgcactgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013697
VERSION     KHUT01013697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           80 g           60 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggaattt
       61 taagtcagat gtgaaatccc cgggcttaac ttgggaattg catttgatac tgtttttcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaagcg gcttcctgga ccaatactga cacttaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013698             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013698
VERSION     KHUT01013698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           53 c          105 g           44 t
ORIGIN      
        1 tacgtaggtg acgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggtggtcggc
       61 caagtctggg gtgaaatcgc ccggctcaac tgggcggggt ccttggaaac tggccgactg
      121 gaggacggta gaggccggtg gaattcctgg tgtagtggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gccggctggg ccggtcctga cactgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013699             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013699
VERSION     KHUT01013699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacctc
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tgggggtctc
      121 gagggaggca ggggcggatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013700
VERSION     KHUT01013700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggacagt
       61 ttagtcagga gttaaagccc agggctcaac cttggaatcg cttttgatac tggctgtctt
      121 gagtacggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013701
VERSION     KHUT01013701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           89 g           55 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggtggtgctc
       61 taagtctgtt gtgaaatctc ccggctcaac tgggagggtg cggcggaaac tggggcgctc
      121 gagtatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagaca gctttctgga ccattactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013702
VERSION     KHUT01013702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           89 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggatcg
       61 caagtcagat gtgaaattct gaagcttaac ttcagcgctg catctgaaac tgtgattctt
      121 gagtgctgga gaggatagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctatctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013703
VERSION     KHUT01013703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           97 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgtgta ggcggtgcgg
       61 taagtcttct gtgaaaactc cgggctcaac ccggagcctg caggggaaac tgccgtgctt
      121 gagtgtggga gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaagcg gctctctgga ccacaactga cgctgagacg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013704
VERSION     KHUT01013704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           91 g           46 t
ORIGIN      
        1 tacgtaaggg gcaagcgttg tccggaatca ctgggcgtaa agcgcacgta ggcggtgtag
       61 caagtcaggg gtgcaagctc caggcctaac ctggaaactg cctctgaaac tactatgctt
      121 gagggtcaga ggggaaactg gaattcacgg tgtagcggtg aaatgcgtag atatcgtgga
      181 gaacacccgt ggcgaaggcg ggtttctggc tgacaactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013705
VERSION     KHUT01013705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           90 g           46 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatca ctgggcgtaa agggtgcgca ggcgggtttg
       61 caagacagat gtgaaagccc caggcttaac ctgggaactg catttgtgac tgcaagtcta
      121 gagtatggca gagggaggtg gaattccatg tgtagcagtg aaatgcgtag agatatggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013706
VERSION     KHUT01013706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          100 g           43 t
ORIGIN      
        1 tacgtacgag gcgagcgtta tccggaatta ctgggcgtaa agcgcgcgca ggtggccgat
       61 taagtcggac gtgaaatctc ccggcctaac tgggaggggt cgttcgagac tggccggctt
      121 gaggactgca gaggtgggtg gaattcctgg tgtagtggtg aaatgcgtag agatcaggag
      181 gaacaccggt ggcgaaggcg gcccactggg cagtcactga cgctgaggcg cgaaagctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013707             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013707
VERSION     KHUT01013707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           96 g           46 t
ORIGIN      
        1 aacgtaggag ccaaacgtta tccggagtta ctgggcgtaa agggcgtgta ggcggttagt
       61 taagtctggc gtgaaagctc tcggctaaac cgggagaggt cgttggatac tggctgactt
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cgctaaagcg cgacagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013708             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013708
VERSION     KHUT01013708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           75 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatca ttgggtttaa agggtccgta ggcggtttgg
       61 taagtcagtg gtgaaagccc atcgctcaac ggtggaacgg ccattgatac tgccagactt
      121 gaattattag gaagtaacta gaatatgtag tgtagcggtg aaatgcttag agattacatg
      181 gaataccaat tgcgaaggca ggttactact aattgattga cgctgatgga cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013709
VERSION     KHUT01013709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttta
       61 caagtcaggg gtgaaatccc acggctcaac cgtggaattg cctttgaaac tgtaaagcta
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01013710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013710
VERSION     KHUT01013710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           85 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggggtgg
       61 taagtcagtg gtgaaatcct gcagcttaac tgtagaattg ccattgatac tgccattctt
      121 gagtacgctg gatgtgggcg gaatgtgccg tgtagcggtg aaatgcttag atatggcaca
      181 gaacaccgat agcgaaggca gctcacaacg gcgaaactga cgctgaggca cgaaagcgtg
      241 gggatcgaac agg
//
LOCUS       KHUT01013711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013711
VERSION     KHUT01013711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           46 c           76 g           56 t
ORIGIN      
        1 tacggagggt ccaagcgtta tccggaatca ttgggtttaa agggtgcgca ggcggtaaag
       61 taagtcagtg gtgaaatccc ttcgctcaac gaaggaactg ccattgatac tgctatactt
      121 gaatacagtt ggagtaggcg gaatgtgtag tgtagcggtg aaatgcttag atattacaca
      181 gaacaccgat agcgaaggca gcttactaaa ctgagattga cgctcatgca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013712
VERSION     KHUT01013712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           95 g           46 t
ORIGIN      
        1 tacgggggga gcaaacgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtcgcg
       61 taagtagggc gtgaaatccc acagcttaac tgtggaactg cgtcctatac tgcgtggctt
      121 gaggatcgga gaggaatcgg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg cgattctggc cgaatcctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013713
VERSION     KHUT01013713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 caagtcaggg gtgaaatact tcagcttaac tggagaagtg cctttgatac tggcgagcta
      121 gagcgaggta ggggcagatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013714
VERSION     KHUT01013714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctt
       61 caagttcggg gtgaaatctt ccggcttaac cggaaaacag ccccggatac tggaggtctc
      121 gaggggggta ggggcggccg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ggccgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013715
VERSION     KHUT01013715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           92 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggcgagg
       61 tcagtcgaat gtgaaatctc ccggctcaac tgggaagggt cattcgatac tgcctggctc
      121 gaagatagga gagggaagcg gaatttcagg tgtagtggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg gctttctggc ctattcttga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013716
VERSION     KHUT01013716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttggatga
       61 caagatgggt gtgaaatccc cgggcttaac ctgggaactg catccaagac tgttatccta
      121 gagtgcggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ccagcactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013717
VERSION     KHUT01013717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           86 g           49 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggcgggg
       61 aaagtttaag gtgaaatctc caggctcaac ttggaaaatg cctcgaatac ttcctcgcta
      121 gagggtcgca gaggaggttg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacatcagt ggcgaaagcg aatctctggg tgacacctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013718
VERSION     KHUT01013718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           85 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ttgggcataa agcgcatgta ggtggtttgg
       61 taagtttgat gtgaaatccc cggactcaat tcgggaagtg cattgaatac tgccaggcta
      121 gagtatggga gaggtgagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacagt ggcgaaggcg gctcactgga ccataactga cactgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013719
VERSION     KHUT01013719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           82 g           58 t
ORIGIN      
        1 tacgtagggt gcaagcgtta ctcggaatta ttgggcgtaa agcgcatgta ggtggttttc
       61 taagtttgat gtgaaatccc tggactcaat tcaggaactg cattgaatac tggaagacta
      121 gagtatggga gaggtaggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg gcttactgga ccataactga cactgagatg cgacagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013720
VERSION     KHUT01013720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           96 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcggat
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaaggg ccttggatac tgatctgctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013721
VERSION     KHUT01013721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgtta ttcggaataa ctgggcgtaa agggcgtgta ggtggctcaa
       61 gaagtctggc gttaaatctc tcggctcaac cgagagctcg cgttggaaac cattgagctt
      121 gagtcctgca ggggtaagcg gaattcccgg tgtagcagtg aaatgcgttg atatcgggag
      181 gaacacctat ggcgaaggca gcttactagg caggtactga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013722
VERSION     KHUT01013722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           84 g           50 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agcgcatgca ggtggaccta
       61 aacgtgagtg attaaagacc gccgcctaac ggcgggaatg ttattcacac ggtaggtctt
      121 gaggatggca ggggtaagcg gaactttgag tgtaggagtg atatccgtag atattcaaag
      181 gaacaccaat ggcgaaggca gcttactggg ccatttctga cactcagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013723
VERSION     KHUT01013723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           77 g           58 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcgggctta
       61 taagtcagag gtgaaatccg gcagctcaac tgtcgaactg cctttgatac tgtaagtcta
      121 gagttaagtt gaggcaggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat agcgaaggca gcctgccaaa cttatactga cgctgatgca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013724
VERSION     KHUT01013724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agcgtgtgca ggcggcctca
       61 caagtggtgt atgaaatatc caggctcaac ctggaggggt tatgccagac tgtgaggcta
      121 gagtgcgaga gaggggcgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcgccctggc tcgcaactga cgctcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013725
VERSION     KHUT01013725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           87 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggaaggta ggtggttacg
       61 tatgtctggg gtgaaatccc tgagctcaac tcaggaagtg ccttggaaac ggcgtaacta
      121 gaatactgga gaggttcgta gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaataccaga ggcgaaggcg gcgaactgga cagttattga cactaaactt cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013726
VERSION     KHUT01013726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           54 c           92 g           39 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggctccg
       61 cacgtccgat gtgaaagccc agggctcaac cctggaagtg cataggatac gacggagctg
      121 gagtgctgaa gaggagagcg gaactcccgg tgtagaggtg aaattcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg gctctctggg cagacactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013727
VERSION     KHUT01013727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggttct
       61 taagtatctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgggaatctt
      121 gagccactta ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013728
VERSION     KHUT01013728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           91 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ctgggcgtaa agagtacgta ggcggtttta
       61 caagtcggga gtgaaagccc agggctcaac cttggaattg ctctcgaaac tgtagaacta
      121 gagtcccgga gaggttggcg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccagt ggcgtaagcg gccaactgga cgggaactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013729
VERSION     KHUT01013729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggctgttagg
       61 taagtcttct gtgaaatcta cgggcttacc ccgtaacgtg cagaggaaac tgcttagctt
      121 gagtgtcgga gaggataggg gaatacccgg tgtagaggtg aaattcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg cctatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013730             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013730
VERSION     KHUT01013730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           83 g           57 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggccttg
       61 taagtcaggg gtgaaagacg gcagctcaac tgtcgcagtg cctttgatac tgcagggctt
      121 gaatgaacta gaggcaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccgat tgcgaaggca gcttgctatg gttttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013731
VERSION     KHUT01013731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           90 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agcgcacgca ggcggttctt
       61 taagtttgac gtgaaagctc ccggcttaac tgggagaggt cgttgaagac tgaagagctt
      121 gaggaaatga gaggggtgca gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaataccagt ggcgaaagcg gcaccctagc atttttctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013732
VERSION     KHUT01013732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           89 g           44 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agagtgcgta ggcggcttat
       61 caggcgggat gtgaaatccc tcggcccaac cgaggaactg cgttccgaac cgataggctt
      121 gaggaggata gaggcgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacacctgt ggcgaaagcg gctcgctggg tccttactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013733
VERSION     KHUT01013733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           38 c           92 g           57 t
ORIGIN      
        1 tacggggggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggttgttaga
       61 taagttatct gtgaaattcc tgggcttaac ctggggtggt cagataagac tgtttgactg
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013734
VERSION     KHUT01013734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           91 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggctgag
       61 caagtcagat gtgaaatccc ggggcttaac tccggaagtg catttgaaac tgctcagcta
      121 gagtatagta gaggttggcg gaatacccag tggagaggtg aaattcgtag atattgggta
      181 gaacaccggt ggcgaaggcg gccaactggg ctataactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013735
VERSION     KHUT01013735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           65 c          107 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggactga ctgggcgtaa agcgcgcgca ggcggtgcgg
       61 ggcgtcggtg ctgaaatcgg gccgcttaac ggcccgcggc gtgccgatac gcccgtactg
      121 gagggtgggc gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt ggcgcaagcg gccacctagc ccgcacctga cgctgaggcg cgacagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01013736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013736
VERSION     KHUT01013736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           51 c           86 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttcgg
       61 aaagaaagat gtgaaatccc agggctcaac cttggaatgg catttttaac tcccgaacta
      121 gagtgtgtca gaggggggtg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca gccccctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013737
VERSION     KHUT01013737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           93 g           52 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggtctgg
       61 taagttggga gtgaaagccc ggggcttaac ctcggaattg ctttcaaaac tgccggacta
      121 gagtgtagta ggggatgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccggt ggcgaaggcg gtcatctggg ctacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013738
VERSION     KHUT01013738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           95 g           45 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggattca ctgggcataa agggcgcgta ggcggttccg
       61 aaagtggcat gtgaaatccc tcggctcaac cgaggaagtg cgtgccaaac ttcggttcta
      121 gaggacggta ggggggactg gaactctcgg tggagcggtg aaatgcgtag atatcgagag
      181 gaacaccggc ggcgaaggcg agtccctggt ccgtttctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013739
VERSION     KHUT01013739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           83 g           58 t
ORIGIN      
        1 tacgggggga gctagcgttt ttcggaatta ttgggcgtaa agggcgtgta ggcggttaat
       61 aaagttgaaa gtgaaatccc cgagcttaac tcgggaactg ctttcaaaac tcattagcta
      121 gaggacgata gaggatagca gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaataccggt ggcgaaggcg gctatctgga tcgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013740
VERSION     KHUT01013740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           45 c           81 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggtaata
       61 gaagtcagat gtgaaatcct ggggctcaac cccagaacag catatgaaac tctatttcta
      121 gagggtaggc ggagaaaatg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013741
VERSION     KHUT01013741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggtttta
       61 taagtcggat gtgaaatccc agggctcaac cttggaagtg catccgagac tgtaagacta
      121 gagtttcgga gagggcagtg gaattcccag tgtagaagtg aaattcgtag atattgggag
      181 gaacatcagt ggcgaaggcg actgcctggc tgaagactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013742
VERSION     KHUT01013742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           52 c           75 g           48 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agagcgaaaa gccggtcatg
       61 ttagtccgat gtaaaaccat agggcccaac tctatgaccg cattggaaac ggcgtgactt
      121 gagcggatga gaggtgagca gaacatatgg tgtaggggtg aaatccactg atatcatatg
      181 gaataccaaa agcgaaggca gctcactagc gtcttgctga cggtgcatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01013743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013743
VERSION     KHUT01013743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           54 c           73 g           54 t
ORIGIN      
        1 tcccagcagt ccaagtcgca accacatttg ttgggtctaa aacatccgta gcttgcctag
       61 taagtctctt gtgaaatcct gcctcttaag ggtagaactg caagagatac tactgggcta
      121 gagaccggga gacgtaacga gtactgatag ggaagcggta aaatgtgttg atcctatctg
      181 gactaccaac agcgaaggca cgttacgagc acggatctga cagtgaggga tgaaggctag
      241 gggcgcaaag tgg
//
LOCUS       KHUT01013744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013744
VERSION     KHUT01013744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           98 g           36 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctg
       61 aaagtcaagg gtgaaagccc ccggctcaac cggggaagtg cccctgatac ttcaggcctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013745
VERSION     KHUT01013745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           86 g           48 t
ORIGIN      
        1 tacgagaggt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggttcta
       61 caagtggggt gtgaaagccc tcggctcaac cgaggaactg cgctccaaac tgtagaacta
      121 gaggaagata gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccgga ggcgaaggcg gctcactggg tcttttctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013746
VERSION     KHUT01013746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgg
       61 caagtcaggg gtgaaatact ccggctcaac cggagcagtg cttttgatac tgccgatctc
      121 gaggggtgta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tacctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013747             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013747
VERSION     KHUT01013747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           88 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcaact
       61 caagtcatct gtgaaatctc cgggcttaac tcggaacggt cagatgatac tgttttgcta
      121 gagtgcagaa ggggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013748
VERSION     KHUT01013748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           85 g           50 t
ORIGIN      
        1 tacgaaggga gcgagcgtta ttcggaataa ctgggcgtaa agagcgtgta gatggtgata
       61 taagtcgatt gttaaagatc acggcctaac cgtgaggatg cggtcgaaac tgtattgcta
      121 gagtgcagga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggc ctgcaactga cattcaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013749
VERSION     KHUT01013749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatga ttgggcgtaa agcgcgtgta ggcggcctgt
       61 tatgccggtt gtgaaagccc gtggctcaac cacggaagtg cagtcggaac tgacaggcta
      121 gagtgccgaa gagggtggcg gaatgaccag tgtagaggtg aaattcgtag atattggtta
      181 gaacaccggt ggcgaaggcg gccacctggg aggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013750
VERSION     KHUT01013750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcctcg
       61 catgtccgat gtgaaagccc acctcttaaa ggtggaattg catcggaaac tgcgaggcta
      121 gagtgccgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cggtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013751
VERSION     KHUT01013751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtcgtg
       61 caagtcggat gtgaaagccc acagctcaac tgtggaagtg cattcgatac tgcgcgactc
      121 gagtcccgga gaggatggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccatctgga cggtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013752
VERSION     KHUT01013752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           91 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggtgtaa agggagcgca ggcggggaat
       61 caagtcagct gtgaaaacta cgggcttaac ttgtagactg cagttgaaac tgattttctt
      121 gagtgaagta gaggttggcg gaattccgag tgtagcggtg aaatgcgtgg atattcggag
      181 gaacaccggt ggcgaaggcg gccaactggg ctttaactga cgctgaggct cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013753
VERSION     KHUT01013753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           84 g           47 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggctgcatgg
       61 taagtcagat gtgaaatccg tgggctcaac ccccgaatcg catccgaaac taccaagcta
      121 gagtgctgaa gaggggcttg gaattctcgg tgtagcagtg aaatgcgtag atatcgaaaa
      181 gaacgctcgt ggcgaaagcg aggccctggt cagcaactga cgctgaggca cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013754
VERSION     KHUT01013754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           95 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtccgct gtgaaatccc agggcttaac cctgggcctg cagtgggtac gggcaaacta
      121 gagtgcggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013755
VERSION     KHUT01013755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           93 g           50 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctag
       61 caagtctgag gtgaaatctt tcggcttaac cggaaaagtg ccttggatac tgctagtctc
      121 gagggaggta ggggcagggg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggt ggcgaaagcg ccctgctgga tctcttctga cgctgaggcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013756
VERSION     KHUT01013756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agagctcgta ggcggcttag
       61 caagtcagag gtgaaatgcc agggctcaac cctggaactg cctttgaaac tgctaggctt
      121 gagactcgga ggggatagcg gaattcctaa tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgagtactga cgctgaggag cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013757
VERSION     KHUT01013757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agagctcgta ggcggggatg
       61 cacgtcctgg agtaaatcca actgctcaac ggttggctca tctgggatac ggtatctctt
      121 gaggacagaa gaggagaaca gaattccgag tgtagcggtg aaatgcgtag atattcggag
      181 gaataccggt ggcgaaggcg gttctctggt ctgattctga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013758
VERSION     KHUT01013758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgaaaggt gcaagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcgggttcg
       61 tgtgtcgatt gtgaaagccc caggctcaac ctgggaatgg cagtcgaaac tgcggatctt
      121 gaatacctga gagggtgggg gaattcctgg tggagaagtg aaattcgtag atatcaggag
      181 gaacatcaga ggcgaaggcg cctacctggc aggctattga cgctgaggtg cgaaagtgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01013759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013759
VERSION     KHUT01013759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattca ttgggcgtaa agagcgcgta ggcggccgtg
       61 caagtcggat gtgaaaatcc ggggctcaac cccggacgtg catccgatac tgctcggcta
      121 gaggtaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01013760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013760
VERSION     KHUT01013760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           83 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agagcatgta ggcggttggt
       61 taagtctgtc gttaaatctc cacgcttaac gtggagtcgt cgacggatac tgatcagctt
      121 gagtttggga gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaac ggcgaaagca ggttactgga ccaatactga cgctgagatg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013761
VERSION     KHUT01013761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcacg
       61 cacgtcaggg gtgaaagccc acggctcaac cgtggaagtg cctttgagac ggcgtgtctt
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013762
VERSION     KHUT01013762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           43 c           91 g           55 t
ORIGIN      
        1 gacggagggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggctgtcgcg
       61 tgtgtcagag gtgaaatccc cgagcttaac ttgggaactg cctttgaaac tgcgcgactt
      121 gaatgataga gaggcaagta gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaataccggt ggcgaaggcg gcttgctggc tatttattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013763
VERSION     KHUT01013763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggctgcaata
       61 taagtcggat gtgaaagccc tcagcttaac tgaggaagtg catccgatac tgtatcgcta
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga cgacgactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013764
VERSION     KHUT01013764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccaac
       61 taagtcagac gtgaaatccc acggcttaac cgtggaactg cgtctgatac tggatggctt
      121 gagattggga gagggacgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgtcctgga ccaacactga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013765
VERSION     KHUT01013765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcatgta ggtggcatat
       61 taagtctgat gtgaaagccc tgggcttaac ctgggaattg cgttggagac tgataagcta
      121 gagtcggaca gagggccgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gcggcctggg tccagactga cactgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013766
VERSION     KHUT01013766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c           96 g           40 t
ORIGIN      
        1 gacgtaggga ccgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggccgtg
       61 caagtcggat gtgaaaacca gaggctcaac ctctggcctg cattcgaaac tgcacggcta
      121 gaggcaggta ggggagaggg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg cctctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01013767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013767
VERSION     KHUT01013767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           95 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgtccgca ggcggtctgt
       61 caagttcggg gtgacagttc ctggcttaac caggaaagtg cttcgaaaac tgggagactt
      121 gagggcttca gaggaaggcg gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaacaccgat ggcgtaggca gccttctggg aagtacctga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013768
VERSION     KHUT01013768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggtcttt
       61 taggccggct gtgaaatccc atggcttaac catggaaggg cggccggaac cgggagactt
      121 gagttctgac gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg aagatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013769
VERSION     KHUT01013769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           75 g           59 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattca ttgggtttaa agggtgcgta ggcgtccttg
       61 taagttttcg gtgaaatcct caagcttaac ttgagaactg ccgagaatac tactaggatt
      121 gagtacagtt gaggtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgaccca
      181 gaacaccaat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013770
VERSION     KHUT01013770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           81 g           53 t
ORIGIN      
        1 tacgtagggt tcgagcgtta atcggaatta ttgggcgtaa agcgtgcgca ggcgggaata
       61 caagatagat gtgaaatcct tgagcttaac ttaagaactg catatatgac tgtataccta
      121 gagtgtatca gaggggggta gaattccatg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg aagacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013771
VERSION     KHUT01013771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggtggcttta
       61 taagtcagtg gtgaaagccc ggagcttaac tccggaactg ccattgatac tgtttagctt
      121 gaatcaggta gaggtggatg gaatattaca tgtagcggtg aaatgcttag atatgtaata
      181 gaacaccgat tgcgaaggca gttcactatg cctgcattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013772
VERSION     KHUT01013772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           93 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcttg
       61 taagtcaagg gtgaaatcct ccggctcaac cggagaacag cccctgatac tgcaggtctc
      121 gaggaaggta ggggcatgtg gaacttcggg tggagcggtg aaatgcgtag atatccgaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013773
VERSION     KHUT01013773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           54 c           78 g           51 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agcgtccgca ggtggttcgt
       61 taagtcagcg gtcaaatcac ggggctcaac cccgtaactg ccgctgatac tggcgagctt
      121 gaggccggta gaggtaagcg gaattacagg tgtagcggta aaatgcgttt atatctgtaa
      181 gaacaccaaa ggcgaaagca gcttactgga acggtcctga cactcatgga cgaaagcgtg
      241 gggagcgaaa agg
//
LOCUS       KHUT01013774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013774
VERSION     KHUT01013774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttttt
       61 taagttatct gtaaaatccc tgggctcaac ctgggcatgc cagataagac tgaaagactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013775
VERSION     KHUT01013775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           86 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtctgt
       61 taagtcaggt gtgaaatcct tgggcttaac ctgagaattg catttgaaac tagcagacta
      121 gagtgtggta gagggtagtg gaactcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg gctacctgga ccaacactga cactgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013776
VERSION     KHUT01013776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           87 g           54 t
ORIGIN      
        1 tacgaaggga gcaagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggtttta
       61 taagtcaggt gtgaaagccc cgagcttaac ttgggatgtg catttgatac tgtaagacta
      121 gaggacgaga gaggaaaaag gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccgat ggcgaaggca tttttctggc tcgtatctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013777
VERSION     KHUT01013777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttag
       61 taagtcagat gtgaaagccc ttggctcaac caaggaatgg catttgaaac tgctagacta
      121 gagtatcgga ggggatggcg gaattcccgg tgtagaggtg gaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013778
VERSION     KHUT01013778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggttgtagat
       61 taagttagct gtgaaatccc tgggcttaac ctggggcggt cagctaagac tgatttactc
      121 gagtacggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013779
VERSION     KHUT01013779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           49 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtgatt
       61 taagtctgat gttaaagacc ggggctcaac cccgacatgg catcggaaac tggattgcta
      121 gagtgcggta gaggtcaagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt agcgaaagcg cttgactgga ccgtaactga cgctgaggaa cgaaagccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01013780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013780
VERSION     KHUT01013780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           83 g           54 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggtattg
       61 taagttagat gtgaaatccc cgggctcaac ctgggaactg cgtttaagac tgcaaaacta
      121 gagtactgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013781
VERSION     KHUT01013781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgagtgta ggtggctatg
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cattcgatac tgcatggcta
      121 gagtctgata gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggaa
      181 gaacaccagt ggcgaaggcg gcttcctgga tcaagactga cactgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013782
VERSION     KHUT01013782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           87 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtttat
       61 taagtgagat gtgaaatccc tgggcttaac ccaggaactg catcttatac tgatgaactg
      121 gagtacagta gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actgcctgga ctgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013783
VERSION     KHUT01013783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           40 c           90 g           59 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ttgggcgtaa agagtatgta ggtggtgtcg
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaagac tgcgatgctg
      121 gagtatggga gagggaatcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg ggttcctgga ccattactga cactgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013784
VERSION     KHUT01013784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggttttg
       61 taagtcggat gtgaaagccc cgggcttaac ctgggaatgg cattcgatac tgctcgacta
      121 gagtatggta gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013785
VERSION     KHUT01013785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           53 c           80 g           44 t
ORIGIN      
        1 gacagaggat gcaagcatta tccggaatga ttgcgcataa agcatttgta ggtggctttt
       61 caagtccgtc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagagg gaattttcgg tggagcggtg aaatgcattg agatcagaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgccactga cacagagaga caaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01013786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013786
VERSION     KHUT01013786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           97 g           46 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcttgg
       61 caagtgacgg gtgaaatccc tcggcttaac cgaggatgtg cccgttagac tgtcaggctt
      121 gagaccggga gaggtgagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctcactgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013787             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013787
VERSION     KHUT01013787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           62 c           95 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggattgg
       61 aacgtcggcg cctgaaatcc cccggctcaa ccggggaacg cggcccgaaa cgaccagtct
      121 ggagggacgt aggggggact ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gagtccctgg acgtctactg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013788
VERSION     KHUT01013788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           90 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggtgact
       61 caagtcagag gtgaaagccc ggagctcaac ttcggaattg cctttgaaac taggtcgctt
      121 gaacgtcgga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga cgactgttga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013789
VERSION     KHUT01013789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           95 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggagtta ctgggcgtaa agggcgcgta ggcggtttat
       61 caagtcagat gtgaaagccc tgggcttaac ccgggaatgg catttgggac tgaaagacta
      121 gaggacgaga gaggatgatg gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaacaccggt ggcgaaggcg gtcatctggc tcgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013790
VERSION     KHUT01013790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ttgggcgtaa agcgaacgta ggcggtttct
       61 taagtttggt cttaaagacc ggggctcaac cccgggagtg gactgaatac tggggagcta
      121 gaggatgtta gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg actttctggg acattcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01013791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013791
VERSION     KHUT01013791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           91 g           42 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggacgt
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaactg ctgggcatac tggcgtcctt
      121 gggccaatca ggggttgtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgaa ggcgaaagca gacaactggg attgcgccga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013792
VERSION     KHUT01013792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           85 g           51 t
ORIGIN      
        1 gacgaagggt gcgagcgtta ttcggaatca ttgggcgtaa agggtgcgta gacggcatat
       61 taagtcaact gttaaatcct ccggcctaac cggagacctg cggtagatac tgatgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccattactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013793             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013793
VERSION     KHUT01013793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           78 g           63 t
ORIGIN      
        1 tacgtaggat ccaagcgtta tccggattta ctgggtgtaa agagttgcgt aggtggtctg
       61 ttaagtaaat agtgaaatcg tgtggctcaa ccatgcatcc attatttaaa ctggcagact
      121 tgagagtgag agaggtagat ggaattgcta gtgtaggagt ggaatccgta gatattagca
      181 ggaacaccga tggcgtaggc agtctactgg ctcatttctg acactaaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013794
VERSION     KHUT01013794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           56 c           92 g           43 t
ORIGIN      
        1 tacagggggc gcgagcgttg ctcggaatca ctgggcgtaa agcgtacgca ggcgggtcga
       61 taagtccgtt gtgaaatccc ggggctcaac cccggaaatg cgatggagac tgccgatctt
      121 gagtacggga gaggggagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctccctggc ccgatactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013795
VERSION     KHUT01013795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           51 c           94 g           48 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggtccga
       61 taagtctgct gtgaaagcca ggggctcaac ccttggatgc cggtggatac tgtcgggctt
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013796             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013796
VERSION     KHUT01013796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           67 c          101 g           31 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggagtcc
       61 cccgtccgag gctgaaagcc cccggctcaa ccggggaacg ggcccggaga cgaggactct
      121 ggaggggggt agggggagcc ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gggctcctgg gccccacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013797             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013797
VERSION     KHUT01013797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           53 t
ORIGIN      
        1 tacgaggggt gcgagcgtta ttcggaatca ttgggcgtaa agggtgcgta ggcggcaaat
       61 taagtcagct gttaaatttc ctggcctaac caggaacatg cggtcgatac tgatatgcta
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatatctga cgctgagtgc acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013798
VERSION     KHUT01013798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           95 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcctc
       61 taagtttggt gtgaaatctc ccggctcaac cgggagggtg cgccgaaaac tggggggcta
      121 gagtgcggga tgggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgt ggtgtagacg gctttctgga ccgcaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013799
VERSION     KHUT01013799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacagagacg gcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggcggtccgt
       61 taagtcgggt gtgaaatctc acagcctaac tgtgataggt cattcgaaac tagcgggctc
      121 gaggactgga gaggagactg gaatagtcgg tgtagcggtg aaatgcgtag agatcgacta
      181 gaacaccggt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013800
VERSION     KHUT01013800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggtctg
       61 taagtcagct gtgaaatccc agggcttaac cttggaatgg cggttgaaac tacagatctt
      121 gaatacccaa gagggtgacg gaattccggg tgtagtagtg aaatacgtag atatccggag
      181 gaacaccaga ggcgaaggcg gttacctggt ggtgtattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013801
VERSION     KHUT01013801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttgg
       61 taagatatct gtgaaatccc cgggcttaac ctgggcaggt cagatataac tgctgaactc
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013802
VERSION     KHUT01013802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           88 g           54 t
ORIGIN      
        1 tacggggggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtcatg
       61 caagtcaggg gtgaaatccc agagcttaac tctggaactg cctttgaaac tgcatggcta
      121 gaatgtgtga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctggc acactattga cgctctggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013803
VERSION     KHUT01013803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           82 g           57 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggtttga
       61 taagttggga gtgaaatccc ggggcttaac tccggaattg ctttcaaaac tatcaatcta
      121 gaatatggca ggggacagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaagcg actgtctgga ccattattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013804
VERSION     KHUT01013804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggtcaga
       61 taagtcagtg gtgaaagctt ccggcttaac cgggaaattg ccattgaaac tgcctgactt
      121 gaatatagtt gaagtggatg gaatacatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccaat tgcgaaggca gttcactaag ctataattga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013805
VERSION     KHUT01013805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           95 g           46 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtagga
       61 tacgtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gattttactg
      121 gagtagatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc atcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013806
VERSION     KHUT01013806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           93 g           44 t
ORIGIN      
        1 tacagaggcc acaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcgggtgtg
       61 taagcgttcc gtgaaatccc ggagctcaac tccggaactg cggggcgaac tgcacatctt
      121 gaggcatgta ggggcgggcg gaacaatagg tggagcggtg aaatgcgtag atatctattg
      181 gaacgccgat ggtgaagacg gcccgctggg catgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013807
VERSION     KHUT01013807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           94 g           42 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggaccgt
       61 taagtatccc gtgaaatccc acggctcaac cgtggaactg cggggtatac tggcggtctt
      121 gagagaccta ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccgac ggtgaaaaca gctcgctggg ggtcttctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013808
VERSION     KHUT01013808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           84 g           51 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcatta
       61 aaagtcggat gtgaaattcc ggggctcaac cccggagctg catttgaaac tttaaagcta
      121 gagtatagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggtgaagacg gttttctggg ctaaaactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013809
VERSION     KHUT01013809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 gacggggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgtgtgca ggtggtcttg
       61 aaagttaggt gtgaaatccc cgggctcaac tcgggaagtg cacttaaaac tacaagactt
      121 gagtaaggaa gagggtcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgacctggt cctatactga cactcataca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013810
VERSION     KHUT01013810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           96 g           43 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtacctt gtgaaatcct gcggcttaac cgcagaactg ctgggtatac tggcgatctt
      121 gagccaccta ggggcagccg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgaa ggtgaagaca ggctgctggg ggtgtgctga cgctgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013811
VERSION     KHUT01013811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c          101 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgccta ggcggtcctt
       61 taagtctgcg gtgaaagacc gaggctcaac ctcggggtgt ccgcggagac tggaggactg
      121 gagtatcgga gaggagtgcg gaattcctgg tgtagcggtg gaatgcgtag agatcaggag
      181 gaacaccgat ggcgaaggca gcactctggc cgaatactga cgctgaagcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013812
VERSION     KHUT01013812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 gacggggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtgtgca ggtggtttgg
       61 taagttggat gtgaaagccc cgggcttaac ccgggaagtg cattcaaaac taccaaactt
      121 gagtagggaa gagggtcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgacctggt cctatactga cactcataca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013813
VERSION     KHUT01013813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           74 g           66 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtatt
       61 taagtcagcg gtgaaatctc caggcttaac ctggaaattg ccattgatac tatttatctt
      121 gaattatctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactacg ggtatattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013814
VERSION     KHUT01013814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgca ggcggttgat
       61 taagttagat gtgaaatccc cgggcttaac ctgggaatgg catctaaaac tggtcagcta
      121 gagtcttgta gaggggggta gaattccatg tgtagcggtg aaatgcgtag agatgtggag
      181 gaataccggt ggcgaaggcg gccccctgga caaagactga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013815
VERSION     KHUT01013815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggactta ctgggcgtaa agggcgcgta ggtggaatta
       61 taagtcagtt gtgaaaggcc tgggcttaac cgaggaacag cgattgaaac tgtgattcta
      121 gagttgagta gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt tgcgaaggcg gctctctgga ctcaaactga cactgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13975 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013816
VERSION     KHUT01013816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13975"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           85 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgttcgca ggcgggaaca
       61 taagtcaagt gtgaaatccc tgagcttaac tcaggacgtg catttgaaac tgtgtttctt
      121 gaatgctgga gagggtcgtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaagcg acgacctggc cagacattga cgctcaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013817
VERSION     KHUT01013817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggccgt
       61 taagtgtctt gtgaaatccc ccggctcaac cggggaattg ctgggcatac tggcggtctt
      121 gagcagttca ggggcagatg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaac ggtgaaaaca gtctgctggg aacttgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013818
VERSION     KHUT01013818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           91 g           53 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggtggtctaa
       61 tgcgtctgat gtgaaatacc tgggcttaac ctgggaactg catcagatac ggttaggctt
      121 gagtgtgaga ggggaagatg gaattgcagg tggagaggtg aaattcgtag atatctgcag
      181 gaacaccagt ggcgaaggcg atcttctggc tcacaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013819             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013819
VERSION     KHUT01013819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           78 g           59 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtttgta ggcggaaatt
       61 taagtgagat gcgaaatctt gtggctcaac catatagacg gtatctcata ctgggtttct
      121 agaagatagc agaggcaagt ggaattagta gtgtagcggt aaaatgcgta gatattacta
      181 ggaacaccaa tggcgaaggc agcttgctgg gctatatttg acgctgagaa acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01013820             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013820
VERSION     KHUT01013820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           99 g           36 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggcccgt
       61 taagtgtccc gtgaaatccc ccggctcaac cggggaaccg cggggcatac tggcgggctt
      121 gggccactca ggggcagacg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccgaa ggtgaaaaca gtctgctggg ggtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013821
VERSION     KHUT01013821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c          104 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggcgga
       61 taagtctgct gtgaaagccc ggagctcaac tccggagggt cggcggatac tgttcgtctt
      121 gagggaggca gggagaagtg gaatggctgg tgtagcggtg aaatgcgtag atatcagtcg
      181 gaacaccggt ggcgaaggcg gcttcttggg ccttacctga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013822
VERSION     KHUT01013822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           86 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtgaca
       61 aaagtccgag gtgaaatccc tgagctcaac tcaggacgtg ccttggaaac tttgtcacta
      121 gagtgctaga gaggttcgtg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacatcaga ggcgaaggcg acgaactgga tagatactga cactgaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013823
VERSION     KHUT01013823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           97 g           39 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggtctgc
       61 taagacagag gtgaaagccc cgggcttaac ctgggaatgg cctatgtgac tggcaggctg
      121 gaggatggca gagggaggcg gaatgccacg tgtagcagtg aaatgcgtag agatgtggcg
      181 gaacaccgat ggcgaaggca gcctcctggg ccaatcctga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013824
VERSION     KHUT01013824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           82 g           55 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggattta ctgggcgtaa agggtgtgta ggcggcaaat
       61 caagtctatt gttaaagatc tcggcctaac cgggaaagtg cgatggaaac tgatttgata
      121 gagattggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctgataca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013825
VERSION     KHUT01013825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           85 g           56 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgctgga gaggctatta gaatacctag tgtagtggtg aaatacgtag atattaggtg
      181 gaataccgga ggcgaaggcg gatagctggc cagacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013826             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013826
VERSION     KHUT01013826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           55 c           99 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agggctcgca ggcggctcga
       61 tcaagtcgtg cgtgaaatct ctcggctcaa ctgggagcgg tcgtgcgata ctgatcagct
      121 tgaggccggt agggggaagc ggaattccgg gtgtagtggt ggaatgcgta gatatccgga
      181 ggaacaccag tggcgaaggc ggcttcctgg accggtcctg acgctcagga gcgaaagcgt
      241 gggtagcgat ccgg
//
LOCUS       KHUT01013827             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013827
VERSION     KHUT01013827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           54 c           85 g           46 t
ORIGIN      
        1 tacggagaac tcaagcgtta cccggaatca ctgggcgtaa agcgttgcgt aggcggtttc
       61 gcaagtcgga tcttaaaagc cgaggctcaa cctcggaatt gggtacgata ctacgagact
      121 agaggatggt agaggtgagt ggaattgctg gtgtaggagt gaaatccgta gagatcagca
      181 ggaacaccga tggcgaaggc agctcactgg accattcctg acgctcaggc acgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01013828             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013828
VERSION     KHUT01013828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           82 g           55 t
ORIGIN      
        1 tacgtagagt acaagcgtta tccggattta ttgggcgtaa agcgtatcgc aggtggggat
       61 atacgtggcg ggtgtaagcc ctgagctcaa ctcaggaact gcttgtcata cggtatctct
      121 tgagtctgtt ggagggaact ggaattcttc gtggaggggt gaaatccgta gatacgaaga
      181 ggaacgccaa gggtgaagac aagttcctac ggcagcactg acactcatga acgacagcta
      241 ggggagcaaa aggg
//
LOCUS       KHUT01013829             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013829
VERSION     KHUT01013829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggaatga ctgggcgtaa agggtgcgta ggtggttcag
       61 caagttagta gcgtaactcc ggagctcaac ttcggcacta ctactaaaac tgttggactt
      121 gagtgcagga ggggcaactg gaattcctag tgtagcggtg gaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg agttgctgga ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013830             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013830
VERSION     KHUT01013830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c          100 g           35 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgt
       61 gcaagtcggg agtgaaagcc cggggctcaa ccccggaacg gctcacgaaa ctgcggggct
      121 cgagtgcgga agaggcaatc ggaattccca gtgtagcggt gaaatgcgta gatattggga
      181 agaacaccgg aggcgaaggc ggattgctgg gccgacactg acgctgaggc gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013831
VERSION     KHUT01013831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           83 g           59 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttagt
       61 taagttggat gtgaaatccc taggctcaac caaggaagtg cattcaaaac taattagcta
      121 gaagatgtta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctggg acattcttga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013832             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013832
VERSION     KHUT01013832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggattta
       61 taagtcagat gtgaaattcc aggactcaat tttggagctg catttgaaac tgtaagtcta
      121 agagggtaga cggagaaaac ggaattccac atgtagcggt gaaatgcgtg gatatgtgga
      181 agaacaccgg tggcgaaagc ggttttctag tttatacctg acgctgaggc gcgagagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013833             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013833
VERSION     KHUT01013833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          104 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggtccgca ggcggttgcg
       61 tgcgtttgtg gtgacagcgg tcggcttcac tgaccgagaa ccacgaagac ggcgtagctg
      121 gagggccaga gagggacatg gaactccggg tggagtggtg aaatgcgtag agatccggaa
      181 gaacaccgaa ggcgaaggca atgtcctggc tggtacctga cgctcgggga cgaaagcgag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013834
VERSION     KHUT01013834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           57 c           98 g           42 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggcccgg
       61 taagtcggat gtgaaatccc gtggctcaac cgcggaactg cattcgaaac tgtcgggcta
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01013835             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013835
VERSION     KHUT01013835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           90 g           48 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtcaag
       61 aaagtcggat gtgaaagccc cgggctcaac ccgggaagtg cattcgatac tgcttgactt
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013836
VERSION     KHUT01013836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttgtt
       61 taagtgggtt gtgaaatccc tgggcttaac ctaggaattg cgacttaaac tggacagctg
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actacctgga ctgtaactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013837             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013837
VERSION     KHUT01013837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           43 t
ORIGIN      
        1 gacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggatcct
       61 gcaagtcata agtgaaatgt cactgctcaa cggtgacgct gcttctgaga ctgcggatct
      121 ggggaccggt agaggctggt ggaattgcag gtgtagcggt ggaatgcgta gagatctgca
      181 agaacacccg tggcgaaggc ggccagctgg gccggatccg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13998 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013838
VERSION     KHUT01013838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13998"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           91 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgatg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgcattgctt
      121 gaggactgga gaggagactg gaattcacag tgtagcagtg aaatgcgtag atattgtgag
      181 gaaggccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU13999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013839
VERSION     KHUT01013839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU13999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           74 g           59 t
ORIGIN      
        1 tacagaggtc ccaagcgtta tccggattca ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctc cgggcttaac tcggaaactg ccattgatac tatctgtctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013840
VERSION     KHUT01013840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggattta ctgggcgtaa agcgtgcgta ggcggtttcc
       61 caagttagaa gtgaaagccc agggcttaac cctggaatgg cttttaaaac tgggtcacta
      121 gaatctagga gaggtaaatg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaat ggcgaaagca gtttactgga ctagtattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013841
VERSION     KHUT01013841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtcttg
       61 taagtctgat gttaaatact ggcgctcaac gccagcacgg cattggaaac tgcaaggcta
      121 gagtatggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ccttgctggg ccgttactga cgctgaggaa cgaaagccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01013842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013842
VERSION     KHUT01013842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           80 g           55 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa aaagcgtgta ggtggtttag
       61 ttagttgaaa gtaaaagccc aaggcttaac cttggaaccg ctttcaaaac tgctaagcta
      121 gagtacggta gaggatgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccaag ggcgaaggca atcatctggg ccgtcactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013843
VERSION     KHUT01013843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           89 g           53 t
ORIGIN      
        1 tacgagaggt gctagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggcggcctgt
       61 taagtttgtt gttaaagacc ttggctcaac tgagggagtg caacaaaaac tggcgggcta
      121 gagggtgata gagagaagtg gaattcccgg agtagcggta aaatgcgtag atctcgggag
      181 gaacaccaat ggcgaaggct gcttcttggt tcactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013844
VERSION     KHUT01013844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 gacgtaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcgcgta ggcggccaag
       61 tcagtcgggt gtgaaatccc tcggctcaac cgaggaactg cgcccgatac tgcttggctt
      121 gagggccgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cggtacctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013845
VERSION     KHUT01013845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcggggatg
       61 taagtcagat gtgaaattcc atggctcaac tgtggcgctg catctgaaac tacatttctt
      121 gagtgctgga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctttctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013846
VERSION     KHUT01013846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggcggttctg
       61 caagtccgtt gtgaaagctc ctggcttaac tgggagaggt caatggatac tgcagggctt
      121 gaatcgagga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaagca ggtgactgga ctcgtattga cgctcatcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013847
VERSION     KHUT01013847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggattta ttgggcgtaa agcgagtgca ggcggttatt
       61 taagtctgat gtgaaagccc tcggctcaac cgaggaaggt cattggaaac tgggtaactt
      121 gagtgcagaa gaggagagtg gaactccatg tgtagcggtg aaatgcgtag atatatggaa
      181 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013848             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013848
VERSION     KHUT01013848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           83 g           50 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggagtta ttgggcgtaa agagcgtgta ggcggtctgt
       61 taagttgact gttaaatctc ccggcctaac cgggaaaatg caatcaaaac tggcaagact
      121 agagggcagg agagagaagt ggaattctcg gagtagcggt aaaatgcgta gatctcgaga
      181 ggaacaccga ttgcgaaggc agcttcttgg cctgtacctg acgctgagac gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013849             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013849
VERSION     KHUT01013849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtcttg
       61 tttgtcagag gtgaaatccc tgggcttaac ccaggaagtg cctctgaaac ggcaagactc
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013850             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013850
VERSION     KHUT01013850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcagaatta ctgggcgtaa agggcgcgta ggcggccaat
       61 caagtcagat gtgaaagccc tgggcttaac ctaggaggtg catttgatac tgataggctt
      121 gagatcgaga gaggagagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg actctctggc tcgatactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013851
VERSION     KHUT01013851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacgaggggg gcaagcgtta ttcggaatta ctgggcgtaa agagcgtgga ggtggcctct
       61 taagttattt gttaaatcct acggcttaac cgtaggcccg caagtaaaac tgagaggcta
      121 gaggatgaaa gagaggggtg gaactcctag agtagcggta aaatgcgtgg atcctaggat
      181 gaacgtcagt ggcgaaggcg gcctctcagg tcatatctga cactcgagcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013852
VERSION     KHUT01013852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           83 g           58 t
ORIGIN      
        1 tacgtagggt gcaagcgtta ttcggaatta ttgggcgtaa agcgcatgta ggcggctttg
       61 caagtttgat gtgaaatccc tggactcaat tcaggaactg cattgaatac tgcatggcta
      121 gagtatgaga gaggtaggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg gcttactgga tcataactga cgctgagatg cgacagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013853
VERSION     KHUT01013853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           77 g           62 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ctgggtgtaa agagtgcgta ggcggataga
       61 caagtcagat gtgaaattcc gttgcttaac atcggagctg catttgatac tacctatctt
      121 gaggatagat ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgtaagcg gttttctaat ttattcctga cgctgaggta cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013854
VERSION     KHUT01013854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctctc
       61 taagtctggt gtgaaatccc ggagcttaac tccggaactg cactggatac tggaaagcta
      121 gagggcgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccatctggc tcgcacctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013855
VERSION     KHUT01013855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c          101 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcatgca ggcggtcgga
       61 cgcgttttgt gtgacagcgg atggcttaac tatccgagga tacgaaagac ggtttgactt
      121 gagggctcga gagggacacg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaaa ggcgaaggca gtgtcctggc gagtacctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013856             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013856
VERSION     KHUT01013856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           79 g           54 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggaatta ctgggcgtaa agagttgcgt aggtggcaga
       61 gtaagcaagt agtgaaagcc tggggctcaa ccccttacac attacttgaa ctgctcagct
      121 tgaggacgag agaggttatt ggaattccta gtgtaggagt gaaatccgta gatattagga
      181 ggaacaccga tggcgtaggc agataactgg ctcgttcctg acactaaggc acgaaagcgt
      241 gggtagcaaa cggg
//
LOCUS       KHUT01013857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013857
VERSION     KHUT01013857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           89 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggaatta ctgggcgtaa agagtgtgta ggcggttctc
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg catcggaaac tggagggctg
      121 gagtatggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacctgc ggtgtagaca gctttctgga ccatcactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013858             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013858
VERSION     KHUT01013858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           84 g           55 t
ORIGIN      
        1 tacgagtgct ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggttgtg
       61 ttagtctttt gtcaaattct tcggcttaac cgggggtccg cagaggaaac ggcacaacta
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa tgcgaaggca attcactggc gcactcctga cgctgagaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013859
VERSION     KHUT01013859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           87 g           53 t
ORIGIN      
        1 tacggaggat gcgagcgtta tccggattca ttgggtttaa agggtgcgta ggtggccgat
       61 taagtcagcg gtgaaagtca gtggctcaac cattgaattg ccgttgaaac tggttggctt
      121 gagtacgtac ggggcaggcg gaatgcgtgg tgtagcggtg aaatgcatag atatcacgca
      181 gaaccccgat cgcgaaggca gcctgccagg ccgtgactga cactgatgca cgaaagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01013860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013860
VERSION     KHUT01013860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatta ctgggcgtaa agggtctgta ggcggcggtt
       61 taggtctgat gtgacacctc gcggctcaac tgcgagtatg cattggaaac cgggtcgcta
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013861
VERSION     KHUT01013861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           45 c           78 g           56 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatca ctgggcgtaa agcgaatgta ggtggctttg
       61 aaagtcagat gtgaaagccc tgggcttaac ccgggaagtg cgtctgatac ttcatagcta
      121 gagtgcggga gaggaaagtg gaatttctag tgtaggggtg aaatccgtag atattagaag
      181 gaataccaat ggcgaaggca actttctggc ccgtaactga cactcagatt cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013862
VERSION     KHUT01013862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 gacggggggt cctagcgttg ttcggaatta ttgggcgtaa agcgtgtgca ggcggtctga
       61 aaagttgggt gtgaaagccc cgagcttaac tcgggaagtg cattcaaaac tgtcggactt
      121 gagtaaggaa gagggttgta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccggt ggcgaaggcg gcaacctggt ccaatactga cgctcataca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013863
VERSION     KHUT01013863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           97 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggagacg
       61 gaagtccggt gtgaaagccc acggcttaac cgtggaaggt cgctggaaac tccgtttctt
      121 gagcacgata gagggaagtg gaattgctgg tgtagcggtg aaatgcgtag atatcagcag
      181 gaacacccgt ggcgaaggcg gcttcctgga tcgtagctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013864
VERSION     KHUT01013864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           98 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgggc
       61 caagtcagtg gtgaaagctc gatgctcaac atcgagtcgg ccactgatac tggttcgctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagttgctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013865
VERSION     KHUT01013865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           90 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa aggggaggta ggtggtctcg
       61 tttgtcagag gtgaaatccc taggcttaac ctaggacgtg cctccgaaac ggcgagacta
      121 gagtactgga gaggttagtg gaatacccgg tgtagcggtg aaatgcgtag agatcgggtg
      181 gaacaccaga ggcgaaggcg actaactgga cagttactga cactgaactc cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013866
VERSION     KHUT01013866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           95 g           43 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccagaatca ctgggcgtaa agcgcgcgca ggcggtttgt
       61 tacgttggtt gtgaaagctc ccggctcaac tgggaagggt cgaccaaaac gggcagactg
      121 gagggcagca gaggtgagtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccaaa ggcgaaggca gctcactggg ctgtgcctga cgctgaggcg cgaaggctag
      241 gggagcgaac agg
//
LOCUS       KHUT01013867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013867
VERSION     KHUT01013867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           43 c           99 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagtgtgat gtgaaagccc tgggcttaac ctgggaagtg cgtcgcatac tgggtagctg
      121 gagtttagga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ctatgactga cgctgaggcg cgagagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013868
VERSION     KHUT01013868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtttag
       61 taagttatgt gtaaaatccc tgggcttaac ctgggaaggt cagataagac tgctagacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013869
VERSION     KHUT01013869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggcttcc
       61 taagtcagag gtgaaagcct ggagctcaac tccagaactg cctttgagac tgggtcgctt
      121 gaatccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013870
VERSION     KHUT01013870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           91 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta ttcggaatta ctgggcgtaa agagcgcgta ggcggtctgc
       61 taagtctgat gtgaaagccc tggactcaat ctaggaagtg cattggatac tggcaggcta
      121 gagtacagga gaggagagcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaacaga ggcgaaggcg gctctctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013871
VERSION     KHUT01013871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           84 g           59 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatca ctgggcgtaa agagcatgta ggtggctttg
       61 taagtggatg attaaagacc aacgcttaac gttgggactg ttgtctatac tgcatggctt
      121 gaggataata ggggcaagcg gaactttagg tgtaggggtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttgctagg ttatttctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013872
VERSION     KHUT01013872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 tacgtaggga ccaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcgtgg
       61 taagtcaatt gtgaaatctc cgagcttaac tcggaacggt caattgatac tgctgtgcta
      121 gagtgcagaa agggcaatcg gaattcttgg tgtagcggtg aaatgcgtag atatcaagag
      181 gaacacctga ggcgaaggcg ggttgctagg ctgacactga cgctgatgcg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013873
VERSION     KHUT01013873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           89 g           46 t
ORIGIN      
        1 tacgaagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgtgta ggcggttagc
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaatgg catttgatac tggttaacta
      121 gaatgtggta gagggaggcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacaccaat ggcgaaggca gcctcctggg ccaacattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013874
VERSION     KHUT01013874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           92 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttaat
       61 caagtcaggt gtgaaagccc tgggctcaac ccgggaattg catttgggac tgattgacta
      121 gagacaggaa gaggatgatg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gtcatctggt ccttgtctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013875
VERSION     KHUT01013875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c           97 g           42 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 aaagtcggtt gtgaaatccc agggctcaac cctgggactg cgtccgaaac ttccgggctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013876
VERSION     KHUT01013876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           90 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggcggctgat
       61 cgagtgcggg gtgaaatccc acggcttacc cgtggaactg cttcgcatac tgatcggctg
      121 gaggacggta gaggagagcg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccaaa ggcgaaggca gctctctggt ccgtttctga cgctgaggtg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013877
VERSION     KHUT01013877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           98 g           46 t
ORIGIN      
        1 tacggggagt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctcga
       61 aaagtctgga gtgaaagccc tcggctcaac cgaggaagcg ctttggaaac tgtcgggctt
      121 gagtcctgga ggggcgggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gcccgctgga cagtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013878
VERSION     KHUT01013878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           95 g           49 t
ORIGIN      
        1 gacgtagggg gcaagcgtta tccggaatta ctgggcgtaa agcgcgtgta ggcggttagg
       61 aaagttggtt gtgaaagctc tcggcttaac cgagagaggt catccaaaac ttcctgactt
      121 gagtgaggta gaggagcacg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gtgctctggg ccttaactga cgctgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013879
VERSION     KHUT01013879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           88 g           45 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgcc
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctgggtatac tgggtgtctt
      121 gagccaacca ggggcagccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaac ggcgaaagca ggctgctgga gttgtgctga cactgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013880
VERSION     KHUT01013880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           89 g           55 t
ORIGIN      
        1 tacgtaggag gcgagcgtta tccggattta ttgggcgtaa agtgggcgta ggtggtcttt
       61 caagtcagat gtgaaatctc ccggcttaac tgggaggggt catctgatac tgttggactt
      121 gagtacggca ggagaaaatg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg attttctagg ccgaaactga cactgaggcc cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013881
VERSION     KHUT01013881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           98 g           42 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agagcccgta ggcggacagc
       61 caagttggac gtgaaagctc ccggcttaac tgggagaggt cgtacaaaac tggttgactt
      121 gaggctgaga gaggcgagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggc tcagacctga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013882
VERSION     KHUT01013882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c          102 g           32 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtccct
       61 gaagtctgac gtgaaagctc ccggcttaac cgggagaggc cgtgagaaac tcggggactg
      121 gagggcagca gaggtgggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcccactggg ctgcacctga cgctgagccg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013883
VERSION     KHUT01013883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           45 c           96 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggaatta ctgggcgtaa agcgcacgca ggtggctgga
       61 taagtctgac gtgaaagctc ctggcttaac tgggagaggt cgttggagac tgtttggctt
      121 gaggacagga gaggggtgta gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaataccagt ggcgaaagcg acaccctggt ctgtaactga cactcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013884
VERSION     KHUT01013884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           99 g           42 t
ORIGIN      
        1 tacggaggtt gcaagcgttg ttcggaatga ctgggcgtaa agggagcgca ggcggtttct
       61 taagtccggc gtgaaagccc ggggctcaac cccggcgggt cgccggatac tggggaactc
      121 gagtacggaa gaggcgagtg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaagcg gctcgctggt ccgatactga cgctcaggct cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013885
VERSION     KHUT01013885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           53 c           91 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggtgtaa agcgcatgca ggtggcctgg
       61 caagtatgct gtgaaagccc cgtgcttaac acggggaggc cagtatagac tgccaagctc
      121 gaggactgca gagggtaggg gaattgctgg tgtagtggtg aaatgcgtag agatcagcag
      181 gaataccaaa gccgaaggga cctacctggg cagtatctga cactcaagtg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013886
VERSION     KHUT01013886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           87 a           30 c           67 g           69 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggaatta ttgggcgtaa agcgtctgta ggttgattta
       61 taagtctttt gttaaatatt taagcttaac ttaaaaaatg caaaggaaac tatttatcta
      121 gagtttggta gaggtaaagg gaatttccag tgaaatggtg aaatatttag atattggaaa
      181 gaacaccaag ggcgaaggca ttttactggg ccataactga cactgagaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013887
VERSION     KHUT01013887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggttgttggt
       61 caagttatct gtgaaatacc tgggcttaac ctggggaggt cagataagac tgattgacta
      121 gagtacagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013888
VERSION     KHUT01013888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           52 c          100 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggcccgt
       61 taagtgcagg gtgaaagctc ccggctcaac tgggagaggt ccttgcatac tggcgggctt
      121 gagggagcta gaggagagtg gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg gctttcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013889
VERSION     KHUT01013889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           92 g           49 t
ORIGIN      
        1 gacagaggtg gcgaccgttg ttcggaatca ctgggcataa agcgcgtgta ggcggcattg
       61 taagtcgggt gtgaaatccc tcggcctaac cgaggaattg cgctcgaaac tactttgctt
      121 gaggatcaga ggggagcctg gaattcctgg tggagcggtg aaatgcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg aggctctggc tgatttctga cgctgagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013890
VERSION     KHUT01013890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           52 c           79 g           49 t
ORIGIN      
        1 tacagagacc ccgagcgtta tccggaatta ctgggcgtaa agcgagcgta gtcggcgcta
       61 ctagtctgat gttaaatctc atcgcctaac ggtgaggccg cattagaaac ggtagcacta
      121 gagcgagtca gaggtacacg gaacgtgtgg tgtagcggtg aaatgcattg atatcacacg
      181 gaacaccaaa agcgaaggca gtgtactggg gctttgctga cgataaggct cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01013891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013891
VERSION     KHUT01013891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctaag
       61 taagtcaggt gtgaaagccc ggggctcaac ctcggaagtg cacttgatac tgcatagctt
      121 gagtgtggga gaggctatta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccgga ggcgaaggcg gatagctggc ccaacactga cactcagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01013892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013892
VERSION     KHUT01013892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           40 c           82 g           55 t
ORIGIN      
        1 tacagagagt gcgagcgtta atcggaatca ctgggcgtaa agcgcatgta ggtgggtatt
       61 taagtcggat gtgaaagccc tgggcttaac ctaggaatag cattcgatac tggatatcta
      121 gagtatggta gagagaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gcttcttgga ccaatactga cactaaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013893
VERSION     KHUT01013893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           87 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttatg
       61 taagttagat gtgaaattcc tgggcttaac ctggggactg cgtctgatac tgtgtagcta
      121 gagtcctata gaggaaagcg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctttctgga cagagactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013894
VERSION     KHUT01013894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           90 g           53 t
ORIGIN      
        1 tacggggggt gcaagcgtta ttcggattga ctgggcgtaa agagcacgta ggcggctcac
       61 taagtctgat gtgaaagccc tgggctcaat ccaggaagtg cattggatac tggtttgcta
      121 gagtatagga ggggagagcg gaatttctgg tgtagaggtg aaattcgtag atatcagaag
      181 gaacaacagc ggcgaaggcg gctctctgga ctattactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013895             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013895
VERSION     KHUT01013895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           91 g           53 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ctgggcgtaa agggcgcgta ggcggctcgt
       61 taagtgtgaa gtgaaatccc tagggctcaa cccaggaact gctttacata ctggcgggct
      121 tgaggattgg agaggcttgt ggaattcctg gtgtagcggt gaaatgcgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcaagctgg ccaatttctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01013896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013896
VERSION     KHUT01013896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           89 g           45 t
ORIGIN      
        1 tacgaactgc gcaaacgtta ttcggaatca ctgggcttac agggtgcgta ggcggccaat
       61 caggtccggt gtgaaatccc acagctcaac tgtggaacgg cattggaaac cgattggctt
      121 gagtgagcta gaggtgcacg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacaccaga ggcgaaagcg gtgcactgga gcttgtctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013897
VERSION     KHUT01013897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           97 g           45 t
ORIGIN      
        1 aacgtaggag acgaacgtta tccggagtta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagttcgtt gtgaaatctc tcggctaaac cgggagggtg cgatggatac tggcagacta
      121 gaggacggta gaggcgtgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg acacgctggg ccgtacctga cgctaagacg cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013898
VERSION     KHUT01013898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           93 g           46 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggattta ctgggcgtaa agcgcatgca ggcggtcgga
       61 gaagtcggat gtgaaagctc ctggcttaac taggagaggt cattcgagac tcttcgactc
      121 gagggtggta gaggagagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg ccacacctga cgctcagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01013899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013899
VERSION     KHUT01013899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          100 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 cgagtctgcg gtgaaatccc ccagcttaac tggggaacgg ccgcggatac tggcggcctc
      121 gagggggata ggggcaagcg gaactgtgcg tggagcggtg aaatgcgttg atatgcacag
      181 gaactccggt ggcgaaggcg gcttgctgga tcccttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013900
VERSION     KHUT01013900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           86 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcttgta ggtggttctt
       61 taagttagat gtgaaaaccc tgggctcaac ctgggacgtg catttaatac tggagggcta
      121 gagtatggta gaggagaggg gaattcctgg tgtagcagtg aaatgcgtag agatcaggag
      181 gaacatcagt ggcgaaggcg cctctctgga ctaatactga cactgaaaag cgaaggcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013901             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013901
VERSION     KHUT01013901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c          103 g           32 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggcggg
       61 tacgtcggcg actgaaagcc cccggctcaa ccggggaagg ggtgccgaaa cggccggcct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgccaactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013902
VERSION     KHUT01013902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg ttcggattta ctgggcgtaa agggtgtgta ggcggttatg
       61 caagtcgaat gtgaaatacc ttggctcaac caaggaagtg cattcgaaac tatatagcta
      121 gagttcggga gaggagagcg gaattctcgg tgtagcggtg aaatgcgtgg agatcgagaa
      181 gaacaccagc ggtgaagacg gctctctaga ccggtactga cgcttaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013903
VERSION     KHUT01013903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           51 c           97 g           47 t
ORIGIN      
        1 gacgtaggga gcaagcgttg cccggaatta ctgggcgtaa agagctcgta ggcgggggcg
       61 tgcgtccgag aggaaatctc aaggctcaac cttggggctg tctcggatac ggcgcttctt
      121 gagggtgtga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc acattcctga cgctgaggag cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013904
VERSION     KHUT01013904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttgga
       61 taagtctgat gtgaaatctc cgagctcaac tcggaaatgg catcggatac tattcagctt
      121 gaggattgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caatacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013905             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013905
VERSION     KHUT01013905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           73 g           61 t
ORIGIN      
        1 tacgaaggac ccgagcgtta tccggattta ttgggtttaa agggtgcgta ggcggacttg
       61 caagtcagtg gtgaaatccc tcagcttaac tgaggaactg ccattgaaac tgcttgtctt
      121 gagtacattt gaggtgggcg gaatgtgtca tgtagaggtg aaattcttag atatgacaca
      181 gaacaccaat tgcgaaggca gctcactaaa ctgtaactga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013906
VERSION     KHUT01013906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           66 c          109 g           29 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggtcgat
       61 cacgtcggac gtgaaagccc ccggctcaac cggggcacgt cgtccgcgac gggtcgactg
      121 gaggctgaca gaggggggtg gaatgccggg tggagcggtg aaatgcgtag agacccggcg
      181 gaacaccggt ggcgaaggcg gccccctggg gcagacctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013907             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013907
VERSION     KHUT01013907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           91 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggctat
       61 taagtcggat gtgaaatctc cacggctcaa ccgggaaact gcatccgaaa ctgttagcct
      121 tgaggtatgg agagggaagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacacccg tggcgaaggc ggcttcctgg tcatatcctg acgctgaggt gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013908
VERSION     KHUT01013908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttagg
       61 taagtcggat gtgaaatctc cgagctcaac tcggaaactg cattggaaac tacctggcta
      121 gagggttgga ggggggactg gaatgcttgg tgtagcagtg aaatgcgtag atatcaagcg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgatgca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013909
VERSION     KHUT01013909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           82 g           54 t
ORIGIN      
        1 tacgtagacc tcgagcgtta tccggattta ctgggcgtaa agcgtacgta ggcgttttgt
       61 taagtcttcg gtgaaatctc tccgcctaac ggagaaactg tcgaagaaac tggcagaatt
      121 gagtgtgaca gaggcagacg gaattcgagg tgtagcggta aaatgcgtag atatctcgaa
      181 gaacaccagt ggcgaaggcg gtctgctggg tcaccactga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013910
VERSION     KHUT01013910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           42 c           88 g           54 t
ORIGIN      
        1 tacgggggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgta ggtggtttta
       61 taagtctgtt gtcaaagcgc gaggcttaac ttcgtacagg cagtggaaac tataagacta
      121 gagtgcggta ggggtagagg gaatttccag tgtagcggtg aaatgcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg ctctactgga cctgcactga cactgaggga cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01013911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013911
VERSION     KHUT01013911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           85 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgtgta ggcggatatt
       61 taagtcggat gtgaaatccc tgggctcaac ctaggaactg cattcgatac tggatgtcta
      121 gagtatggta gaggaaagtg gaactcccgg tgtagcggtg aaatgcgtag atatcgggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ccaatactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013912             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013912
VERSION     KHUT01013912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           44 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 taagtcaggt gtgaaaactc tgggctcaac tcagagacgc cacctgatac tgctgtggct
      121 agagtccggt aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcgctctgg gccggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013913
VERSION     KHUT01013913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           98 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtctgt
       61 cgcgtcggct gtgaaatctc ggggctcaac tccgagtctg cagtcgatac gggcagactt
      121 gagtgctgta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg cagtgactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013914
VERSION     KHUT01013914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           60 c          108 g           35 t
ORIGIN      
        1 gacagagggg gcgagcgttg tccggagtga ctgggcgtac agcgcgcgca ggcggatcgt
       61 gacgtgccgt gtcacagccc tgagctcacc tcagggtggc catggtagac gcgcggtctg
      121 gaggcaggga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gcatcctggc cctgacctga cgctgaggcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013915
VERSION     KHUT01013915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgca ggcggctttg
       61 taagtctgat gtgaaagccc cgggcccaac ccgggaagtg cattggatac tgcaagactt
      121 gagtacggga gaggagagtg gaattcctgg tgtaggggtg aaatccgtag atatcaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013916
VERSION     KHUT01013916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggtcctg
       61 tgggtaagat gtgaaatccc tgagcttaac ttaggaagtg catcttaaac tacaagactg
      121 gagtacagta gagggtagcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgccagt ggcgtaggcg gctacctgga ctgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013917
VERSION     KHUT01013917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           84 g           59 t
ORIGIN      
        1 tacgtaggat ccaagcgttg tccggattta ctgggtgtaa agggcgtgta ggtggattct
       61 taagtcggtg gtgaaatctt gcagctcaac tgcaaaactg ccattgatac tgggaatctt
      121 gagtgcggca gagggaaatg gaattcatgg tgtagcggtg aaatgtgtag atatcatgaa
      181 gaacaccggt agcgaaggcg gtttcctggt ccgtaactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013918
VERSION     KHUT01013918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgtacgta ggcggatttg
       61 taagtcgatt gtgaaagccc tgggcttaac ccgggaattg cagtcgaaac tgcaagtctt
      121 gaatgctcga gaggatggtg gaattcctgg agtagaagtg aaattcgtag atatcaggag
      181 gaacatcaga ggcgaaggcg gccatctggc gagacattga cgctaaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013919
VERSION     KHUT01013919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           95 g           40 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccggagtta ctgggcgtaa agcgcgcgta ggcggtccgt
       61 taagtggggc gtgaaagccc cgtgctaaac acggggagga cgaaccatac tgacggacta
      121 gagtagaaca gagggacatg gaattccggg tgtagcggtg aaatgtgcag atatccggag
      181 gaacaccaga ggtgaaaacg gtgtcctggg ttcatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013920
VERSION     KHUT01013920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctggg
       61 tcagtctgct gtgaaagtcc agggctcaac cctggaatgt cggcagatac tgctcagcta
      121 gagtacggga ggggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctgga acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013921
VERSION     KHUT01013921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ttgggcgtaa agggcgcgca ggcggttttc
       61 caagtctgat gtgaaagccc cgggcttacc ccgggaagtg cattggaaac tgggagactt
      121 gagtacggaa gaggagagtg gaattcctgg agtaggggtg aaatccatag atatcaggaa
      181 gaagaccggt ggcgaaggcg gctctctgat acgatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013922
VERSION     KHUT01013922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           95 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggacctt
       61 taagtgggtg gtgaaattcc gaggctcaac ctcggaactg cctctcaaac tgggggtctc
      121 gaggcttgga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catgtcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013923
VERSION     KHUT01013923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agcgtacgta ggcggtttac
       61 caagtctgga gttaaagacc gaggctcaac ctcgggagtg ctctggaaac tggtaggctg
      121 gaggacggca gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaagcg actttctggg ccgttcctga cgctgaggta cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013924
VERSION     KHUT01013924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           89 g           54 t
ORIGIN      
        1 tacgagggat cctagcgttg ttcggaatca ttgggcgtaa agggtgcgta ggtggccaag
       61 taagtcagtt gtgaaagccc cgggctcaac ccgggaagtg cttctgatac tgcatggctt
      121 gaatgttgga gaggtgagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctcactggc catacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013925
VERSION     KHUT01013925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          106 g           40 t
ORIGIN      
        1 tacgtagggg tcgagcgttg tccggagtta ctgggcgtaa agcgtgcgca ggtggctcat
       61 tgcgcccggc gtgaaagccc ccggctcaac cggggagggt cgtcggggac gggtgagctt
      121 gagggtatca ggggctggtg gaactcccgg tgtagtggtg aaatgcgtag agatcgggaa
      181 gaacacccgt ggcgaaggcg gccagctggg atacacctga cactgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013926
VERSION     KHUT01013926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           60 c          100 g           42 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggccgt
       61 gtggtccggg gtgaaatcct cccgcttaac gggagaacgg ccccggatac ctgcggtctc
      121 gaggagggta ggggcgtgcg gaacactggg tggagcggtg aaatgcgttg atatccagtg
      181 gaactccggt ggcgaaggcg gcacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013927             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013927
VERSION     KHUT01013927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgtgta ggcggtcttg
       61 taagtcggat gtgaaagccc cgggctcaac ctgggaactg cattcgatac tgcaggacta
      121 gagtatggaa gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctggt ccaatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013928
VERSION     KHUT01013928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgat
       61 taagttggtt gtgaaagccc tgggctcaac ctgggaaggc caactaagac tgatgaactg
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgttactga cactgaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013929
VERSION     KHUT01013929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           86 g           46 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggtaccg
       61 taagtcgtat gtgaaatccc ggggctcaac cccggaacag catatgaaac tgcggttcta
      121 gaggataggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttagacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013930
VERSION     KHUT01013930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           84 g           53 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggtggtctgg
       61 aaagttggat gttaaatctt tcggctcaac cgagaggtgt cgttcaaaac tcccagactg
      121 gaggacggta gaggtaggta gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaataccagt ggcgaaggcg gcctactgga ccgttcctga cactcagaca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013931
VERSION     KHUT01013931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           97 g           41 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatga ctgggcgtaa agcgcgtgta ggcggccgtg
       61 cacgtcgggt gtgaaacccc ggggctcaac cccgggcgtg cattcgatac gacacggctg
      121 gaggcctgta gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggc caggttctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013932
VERSION     KHUT01013932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           95 g           50 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggagcg
       61 tgcgtccgag aggaaatctc aaggctcaac tttggggctt tctcggatac ggcgcttctt
      121 gagggtaaca gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctggg ttattcctga cgctgaggag cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013933             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013933
VERSION     KHUT01013933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           52 c          101 g           49 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcttac
       61 caagtcggtc gtgaaatctc tcggctcaac cgggaggggt cgggcgaaac tggtaggctt
      121 gaggatggta ggggcaagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttgctgga ccatttctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01013934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013934
VERSION     KHUT01013934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           83 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaaga
       61 aaagtcggat gtgaaatccc ggggctcaac ctcggaactg catttgaaac tctctttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaggcg gttttctagc ttacacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013935
VERSION     KHUT01013935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           95 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgg
       61 tcggtcaggg gtgaaatcct ccggctcaac cggagaacag cctttgatac cgccagtctc
      121 gaggaaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccttttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013936
VERSION     KHUT01013936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           96 g           43 t
ORIGIN      
        1 tacgtagggg acgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggctctg
       61 caagtcctgc gtgaaatctc gaggctcaac ctcgagcggt cgtgggaaac tggagggcta
      121 gagtgcggga gagggaagtg gaattccagg tgtagcggtg aaatgcgcag atatctggaa
      181 gaacacccgt ggcgaaggcg gcttcctgga acgacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013937
VERSION     KHUT01013937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           84 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgca ggctgtggtg
       61 taagtcgagt gtgaaatctc ggggctcaac cccgaaattg cactcgatac tgcatcacta
      121 gaggactgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaagcg actctctggg cagttcctga cgctcatgca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013938
VERSION     KHUT01013938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           86 g           49 t
ORIGIN      
        1 gacgagccac acaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtgcgg
       61 aaagtcgggt gtgaaatccc tcagcttaac tgaggaactg cgcccgatac taccgtgcta
      121 gagggagata gaggaatgcg gaacttaggg tggagcggtg aaatgcgttg atatcctagg
      181 gaacaccggt ggcgaaagcg gcattctgga tctcttctga cgctgaggca cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01013939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013939
VERSION     KHUT01013939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           92 g           47 t
ORIGIN      
        1 aacgtaggtt ccgagcgtta tccggattta ctgggcgtaa agcgcgttca ggcggctggt
       61 caagtcgggc atgaaagctc tcggctcaac cgggagaggc tgtccgatac tgatcggctt
      121 gagggcagta gagggtggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccacctgga ctgtacctga cgctcaaacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01013940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013940
VERSION     KHUT01013940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           84 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcgggtctg
       61 taagtcagtg gtgaaagcct tcagcttaac tggagaattg ccattgatac tgctgacctt
      121 gaattcggtt gaagtgggcg gaatgtgtgg tgtagcggtg aaatgcatag atatcacaca
      181 gaacaccgat tgcgaaggca gctcactaag ccgatattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01013941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013941
VERSION     KHUT01013941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctgag gtgaaagccc acggcttaac cgtggaagtg ccccggatac tggtagtctc
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013942
VERSION     KHUT01013942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           99 g           36 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggcatcg
       61 cacgtcgggt gtgaaagccc ggggctcaac cccggcagtg cacccgaaac gacgatgcta
      121 gagtgctgga gaggcgggtg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcccgctggc cagcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013943
VERSION     KHUT01013943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggattta ctgggcgtaa agcgcttgta ggcgggctag
       61 cgtgtcgggt gtgaaatccc tgggcttaac ccaggaactg cattcgaaac tactagtctt
      121 gaggaattga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga tgcgaaggcg gctctctgga aattatctga cgctgagaag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013944
VERSION     KHUT01013944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           89 g           43 t
ORIGIN      
        1 gacgtagggg acgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggctgcg
       61 caagtcggat gtgaaaatcc ggagctcaac cccggacctg catccgatac tgcgcggcta
      121 gaggtaggta ggagagatcg gaattcctag tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaagcg cgaaagctaa
      241 ggaaacgaac agg
//
LOCUS       KHUT01013945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013945
VERSION     KHUT01013945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           83 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcataa agggcgcgta ggcgggcctg
       61 caagtcagat gtgaaatccc ggagctcaac ttcggaacag catttgaaac tacaggtctt
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacatcggt ggcgaaggcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01013946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013946
VERSION     KHUT01013946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           49 c           84 g           49 t
ORIGIN      
        1 tacggagggt gctaacgttg tccggaatta ctgggcgtaa agcgcatgta ggtggtctcg
       61 cacgtcagtg atcaaagact gtcgcttaac gacagaaacg ttactgatac gacgaggctt
      121 gaggaagaca ggggtacgcg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca gcgtactggg tcttacctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013947
VERSION     KHUT01013947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           89 g           52 t
ORIGIN      
        1 tacagagatc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggctgttccg
       61 caagtgcttt gtgaaatccc atggcttaac catggaattg ctcggtagac tgcggaactt
      121 gaggtcggta ggggctgccg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccaaa ggtgaagaca ggcagctggg ccgattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01013948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013948
VERSION     KHUT01013948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           91 g           53 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgca ggcggattga
       61 ttagtcgggg gtgaaatccc ggggctcaac ctcggaattg ccttcgatac tgtcagtctt
      121 gagtccggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013949
VERSION     KHUT01013949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgtaggcc acaagcgtta tccggattta ttgggcgtaa agcgtgtgta gggggtttgg
       61 tgcgtctgtg gttaaagccc accgcctaac ggtggaagtg ccacggatac gaccagactg
      121 gagggtgtta gaggtgcacg gaacacatgg tgtaggggtg aaatccgttg atatcatgtg
      181 gaacaccgaa ggcgaaggca gtgcactggg acattcctga ccctgagaca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01013950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013950
VERSION     KHUT01013950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           49 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggcttga
       61 tacgtctgat gtgaaatccc tgggcttaac ctaggaacag cattggatac ggttgagcta
      121 gagtccgcga gaggaagatg gaattgtgcg tgtagaggtg aaattcgtag agatgcacaa
      181 gaacaccggt ggcgaaggcg atcttctgga gcgagactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013951
VERSION     KHUT01013951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ctgggcgtaa agggttcgta ggcgggaatg
       61 caagtcaagt gtgaaatccc caggcttaac ctgggacgtg catttgagac tgtgtttctt
      121 gagtttcgga gagggtggtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaggcg accacctggc cgaatactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013952
VERSION     KHUT01013952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           87 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggcggtcggg
       61 taagtctgat gtgaaatctc cgagcctaac tcggaaacgg catcggatac tacttggctt
      121 gaggaatgga agggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga catttcctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013953
VERSION     KHUT01013953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           59 c           76 g           45 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agcgtgagcc accggtcgtg
       61 ttagtctgat gtaaaaccac agggcccaac tctgtgtccg catcggaaac ggcacgactc
      121 gagcggatga gaggagagcg gaacgtatgg tgtaggggtg aaatccactg atatcatacg
      181 gaacaccaaa agcgaaggca gctctctagc gtcttgctga cggtcaatca cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01013954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013954
VERSION     KHUT01013954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           80 g           57 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ctgggcgtaa agcgtgtgta ggcggttttg
       61 ttagtctttc gttaaattct tcggcttaac cgggggcatg cgaaggaaac ggcaaaactt
      121 gaaggtgcaa ggggtttatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtaaactggt gcactcttga cgctgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01013955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013955
VERSION     KHUT01013955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggattta ctgggcgtaa agcgtgcgta ggctgtattg
       61 agagtcgcgg gtgaaatccc agggcttaac cctggaactg cctgcgagac ctcagtacta
      121 gagtgtgaga ggggatagtg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacaccggt ggcgaaggcg gctatctggc tcacaactga cgctcaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013956             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013956
VERSION     KHUT01013956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           84 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaaa
       61 taagttagat gtgaaatccc cgagcttaac ttgggaacgg catataatac tgtttagcta
      121 gagtagaata gaggggagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttcctgga ttcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01013957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013957
VERSION     KHUT01013957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcttga
       61 taagtcagac gtgaaatccc ttggcttaac caaggaactg cgtctgatac tgtcaggcta
      121 gagtgtcgga ggggatggtg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg accatctgga agacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013958
VERSION     KHUT01013958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           59 c          103 g           38 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggacggg
       61 taagtgtggc ttgaaagccc ccggctcaac cggggaaggc ggctgcaaac tgcccgtctt
      121 gagggcgaca gaggagtgtg gaattccggg agtagcggtg aaatgcgtag agatccggag
      181 gaacacccgt ggcgaaggcg gcactctggg tcgcccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013959
VERSION     KHUT01013959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctaa
       61 taagtcggtc gtgaaatccc ttggcttaac caaggaactg cgtccgaaac tgttgggctc
      121 gagtatcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga agactactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013960
VERSION     KHUT01013960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           84 g           54 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggctgttctg
       61 caagtcagcg gtgaaagccc ggggctcaac cccggaaatg ccgttgatac tgcggggctg
      121 gaatacggat gccgtgggag gaatgagtag tgtagcggtg aaatgcatag atattactca
      181 gaacaccgat tgcgaaggca tctcacgaat ccgttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013961
VERSION     KHUT01013961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgca ggcggtcctg
       61 caagtctgat gtgaaagccc ggggctcaac cccgggactg cattggaaac tgtgggactt
      121 gagtgtcgga ggggcaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttgctgga cgacaactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013962
VERSION     KHUT01013962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           85 g           55 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggtgcgta ggcgggaagg
       61 taagtcagat gttaaattcc ggggctcaac cccggaccgg catttgaaac tgcttttctt
      121 gaggataggt ggggaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccggt ggcgaaggcg gttttctaac ttattcctga cgctgaggca cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013963
VERSION     KHUT01013963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacggagggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcgggtttg
       61 taagtcaatt gtgaaatccc taggctaaac ctgggaattg cagttgaaac tgcgaatctt
      121 gaatgcttaa cagggtagcg gaattcctgg tgtagaagtg aaattcgtag atatcaggag
      181 gaacaccaga agcgaaagcg gctgcctggc aatgcattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013964
VERSION     KHUT01013964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 tacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgtgg
       61 caagtcggta gtgaaatctc caggcttaac ttggaggctg ctgccgaaac tgccgtgcta
      121 gagtacggga gaggcgagtg gaattacagg tgtagcggtg aaatgcgtag atatctgtag
      181 gaacacccgt ggcgaaggcg gctcgctgga ccgttactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013965             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013965
VERSION     KHUT01013965.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcgggttac
       61 taagtcaggg gtgaaatgcc gaggctcaac ctcggaactg cctttgatac tggtaacctg
      121 gagtacggaa gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggt ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013966             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013966
VERSION     KHUT01013966.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           86 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttgg
       61 taagtctgtt gtgaaagctt cctgcttaac ggggagaggc caacagatac tgccggactt
      121 gagtatggga gaggttactg gaattcctgg tgtagcggtg aaatgcgcag agatcaggag
      181 gaacaccgat ggcgaaagca ggtaactgga ccattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01013967             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013967
VERSION     KHUT01013967.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggtttcg
       61 cacgtccgat gtgaaagccc agggctcaac cctggaagtg cattggatac gacggagctg
      121 gagtgctgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagcgactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013968             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013968
VERSION     KHUT01013968.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgtta
       61 caagtcgggt gtgaaatccc tcagcttaac tgaggaactg cgctcgaaac tgtagtgcta
      121 gagggagaca ggggtgagcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctcactggg tctctactga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01013969             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013969
VERSION     KHUT01013969.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           93 g           50 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtcagt
       61 caagtctgat gtgaaatccc tgggctcaac tcaggaattg cattggatac tagctggctt
      121 ggagtactgg agagggaagc ggaatttctg gtgtagcggt gaaatgcgta gataccagaa
      181 ggaacaccgg tggcgaaggc ggcttcctgg ccaagtactg acgctgaggc acgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01013970             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013970
VERSION     KHUT01013970.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttctt
       61 taagtcagga gtgaaagccc tgggctcaac ccgggaattg ctcttgaaac taaggaactc
      121 gagtgtagga gaggtgagtg gaattcccag tgtagaggta aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctcactggc ctactactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013971             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013971
VERSION     KHUT01013971.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           82 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgca ggtggtattt
       61 taagtgtact gtgaaatgcc aaggcttaac cttggaattg cattacatac tggagtacta
      121 gagtttagta gaggatagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt tgcgaaggcg gctatctgga cataaactga cactgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01013972             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013972
VERSION     KHUT01013972.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           95 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcacgca ggcggtttgg
       61 aaagtccgtg gtgaaatctc tcggctcaac cgagaggggt ctgcggatac tcccagactt
      121 gagtggcgta gacgaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccttcgagg cgccaactga cgctgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01013973             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013973
VERSION     KHUT01013973.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           89 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtatat
       61 taagttatct gtgaaatacc tgggcttaac ctgggcaggt cagataaaac tgatgtactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013974             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013974
VERSION     KHUT01013974.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14136"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           86 g           54 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggcaggta ggtggttatg
       61 taagtctggt gtgaaatccc agagctcaac tctggaactg cattggaaac tacatgactt
      121 gaatatcgga gaggtgaggg gaattcccgg tgtaagggtg aaatctgtag atatcgggag
      181 gaacaccagt ggcgaaggcg cctcactggc cgattattga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01013975             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14137 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013975
VERSION     KHUT01013975.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14137"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           45 c           84 g           51 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggagtta ttgggcgtaa agagcgcgta ggcggttcgt
       61 taagtcaact gttaaatatc ccggcccaac cgggaaaatg cagtcgaaac tggcagacta
      121 gagtgtaaga gagagaagta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gcttcttggc ttataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013976             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14138 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013976
VERSION     KHUT01013976.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14138"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           95 g           42 t
ORIGIN      
        1 gacgtaggga gcgagcgtta tccggattca ttgggcgtaa agcgcacgca ggcggccaag
       61 taagtcgggt gtgaaatccc ggggctcaac cccgggcgtg catccgaaac tgcttggcta
      121 gaggtaggca ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttacctga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01013977             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14139 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013977
VERSION     KHUT01013977.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14139"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggattgg
       61 aaagttggag gtgaaatccc agggctcaac cttggaactg ccttcaaaac tcccagtctt
      121 gagttcgaga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccagt ggcgaaggcg gctcactggc tcgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013978             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14140 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013978
VERSION     KHUT01013978.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14140"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           48 c           95 g           53 t
ORIGIN      
        1 tacgtgaggg gcgaacgttg ttcggtgtca ctgggcttaa agggtgcgta ggtggttgcg
       61 taagtaacgt gtgaaatccc tcggcttacc cggggaattg catgttatac tgcgtgactt
      121 gaggaccaga ggggaaaagg gaactccagg tgtagcggtg aaatgcgcag atatctgggg
      181 gaaggccggc ggcgaaggcg cttttctggc tggttcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013979             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14141 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013979
VERSION     KHUT01013979.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14141"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcttct
       61 caagtcaggg gtgaaatcct tgggctcaac ccaagaattg cctctgaaac tgtggagctg
      121 gagtactgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagtaactga cgctgaggcg cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013980             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14142 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013980
VERSION     KHUT01013980.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14142"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggtggtttgg
       61 taagtctgtt gtgaaagctc ccggcttaac cgggagaggt caatggatac tgccgaactt
      121 gaatgtggga gaggtgactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaat ggcgaaagca ggtcactgga ccactattga cactcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01013981             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14143 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013981
VERSION     KHUT01013981.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14143"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           87 g           56 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgtgta ggcggtcgag
       61 atagtctgat gttaaatctc tgggctcaac ccagatgccg cattggatac ttcttggcta
      121 gagaggcata gggggaaccg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacgccagt ggcgaaagcg ggtttctgga tgccttctga cgctgaggca ctaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01013982             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14144 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013982
VERSION     KHUT01013982.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14144"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           85 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggattta ctgggcgtaa agcgtacgca gacggtaagg
       61 taggtcgggt gtgaaagccc tgggctcaac ccaggaactg catccgaaac ccccttgcta
      121 gagtatgaga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatcagc ggcgaaggcg gcttcctggc tcattactga cgttcaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01013983             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14145 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013983
VERSION     KHUT01013983.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14145"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaataa ctgggcgtaa agggcgcgta ggtggttatg
       61 taagatcgat gtgaaatccc tgggcttaac ctaggaactg catcgatgac tggatagctg
      121 gagtactgta gagggtagta gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaataccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013984             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14146 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013984
VERSION     KHUT01013984.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14146"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcttaa
       61 taagttggga gtgaaatccc ggggcttaac tccggaattg ctttcaaaac tattaagcta
      121 gagtatggca ggggacagtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaagcg actgtctgga ccattactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013985             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14147 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013985
VERSION     KHUT01013985.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14147"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           60 c          108 g           38 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggttttt
       61 caggcccgac gtgaaagccc tcggctcaac cggggagagt cgtcggggac cgggaaactt
      121 gagggcggca ggggtcggtg gaatccccgg tgtagtggtg aaatgcgtag agatcggggg
      181 gaacacccgt ggcgaaggcg gccgactggg ccgactctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013986             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14148 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013986
VERSION     KHUT01013986.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14148"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           93 g           42 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttatc
       61 caagacaggc gtgaaatccc cgggctcaac ctgggaacgg cgcttgtgac tggatggcta
      121 gagtacggca gaggggggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg ccgatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013987             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14149 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013987
VERSION     KHUT01013987.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14149"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agagcgcgta ggcggctatg
       61 taagtcagag gtgaaatccc agggctcaac cctggaactg cctttgaaac tacatggcta
      121 gagttcgtga gagggtggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gccacctggc acgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013988             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14150 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013988
VERSION     KHUT01013988.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14150"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatca ctgggcgtaa agggtgcgta ggtggttgta
       61 taagtttcat gtgaaatccc tgggcttaac ctgggaactg catgggagac tgtatgacta
      121 gagtagagta gaggtgagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctcactgga ctcatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01013989             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14151 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013989
VERSION     KHUT01013989.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14151"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           98 g           46 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agggtgtgca ggcggggggt
       61 caagtggtgt atgaaagggc gtggctcaac cacgctaggt tatgccagac tggctctctt
      121 gaggacgaaa cagggctgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaaccccaga ggcgaaggcg gcagcctggt tcgtacctga cgctcggaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01013990             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14152 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013990
VERSION     KHUT01013990.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14152"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           90 g           49 t
ORIGIN      
        1 tacagagggc ccgagcgttg cgcggaatca ctgggcttaa agggtgcgta ggcggaagcg
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaattg ctgggcatac tgcgcttctt
      121 gaggtcggta agcgctgccg gaacagtagg tgtagcggtg aaatgcgtag atatctactg
      181 gaacgccgat ggtgaagacg ggcagctagg ccgattctga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01013991             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14153 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013991
VERSION     KHUT01013991.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14153"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           90 a           51 c           68 g           44 t
ORIGIN      
        1 tacgggagga gcaagcgttg ttcggaatta ctaggcgtaa aaggctcgta ggcggccaac
       61 taagtcaaac gtgaaatccc tcggcttaac cgaagaactg cgcttgatac tgaatagctt
      121 gagattggga aaaagatgcg gaattccagg tgtaacggtg aaatgcgtag atatctaaaa
      181 aaacaccaat gacgaaaaca gcatcctaga ccaacactga cgctgagaag cgaaagccaa
      241 aggagcaaac ggg
//
LOCUS       KHUT01013992             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14154 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013992
VERSION     KHUT01013992.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14154"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           91 g           52 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttttg
       61 taagtcgcat gtgaaatctc tgggcttaac ccagaggggc catgtgatac tgcaatgact
      121 tgagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccaa tggcgaaggc agcactctgg gccgcgactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01013993             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14155 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013993
VERSION     KHUT01013993.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14155"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           52 c           85 g           47 t
ORIGIN      
        1 tacggagaac tcaagcgtta cccggaatca ctgggcgtaa agcgttgcgt aggcggactt
       61 gtaagtcgga tcttaaaagc cggggctcaa cctcggaatt ggatacgata ctgcaagact
      121 agaggatggt agaggtgagt ggaattgctg gtgtaggagt gaaatccgta gatatcagca
      181 ggaacaccaa tggcgaaggc agctcactgg gccattcctg acgctgaggc acgaaagcgt
      241 ggggagcgaa ccgg
//
LOCUS       KHUT01013994             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14156 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013994
VERSION     KHUT01013994.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14156"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggcc tctgaaatcc cccggctcaa ccggggaagt ggagccgata cgacccgtct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctgctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01013995             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14157 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013995
VERSION     KHUT01013995.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14157"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           62 c           87 g           43 t
ORIGIN      
        1 caccagcgcc acaagtggct atcacgatta ttgggcctaa agcgtccgta gtcggtcatg
       61 cgcatcctct gtgaaatcgc agcgcttaac gttgcggcat gcgggggaca cggcatggct
      121 tgagaccggg aggcgtcgag ggtattccaa ggggaccggt aaaatggtat aatccttgga
      181 ggaccacctg tggcgaaggc gctcgacgag aacggatctg acgatgaggg acgaaagcca
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01013996             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14158 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013996
VERSION     KHUT01013996.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14158"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           54 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgg
       61 caagtcgacg gtgaaatctc cacgctcaac gtggaggggt ccgtcgatac tgccgtgact
      121 ggagtccggt agaggagtgt ggaattcccg gtgtagcggt gaaatgcgca gagatcggga
      181 ggaacacccg tagcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013997             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14159 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013997
VERSION     KHUT01013997.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14159"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           56 c          108 g           44 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa aaggcttgta ggcggccacc
       61 gcgcgtcgtg cgtgaaatct cccggctcaa ctgggagggg tcgtgcgata cggcggggct
      121 cgaggccggt agggggacgc ggaattcccg gtgtagtggt ggaatgcgta gatatcggga
      181 ggaacaccag tggcgaaggc ggcgtcctgg accggtgctg acgctgagga gcgaaagcgt
      241 ggggagcgat ccgg
//
LOCUS       KHUT01013998             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14160 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013998
VERSION     KHUT01013998.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14160"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           48 t
ORIGIN      
        1 cacgtagggg ccaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcag
       61 tcagttgggt gtgaaaactc cgggcttaac tcggagaggc cacccaatac tgctgtgact
      121 tgagtccggt aggggagtgg ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gccactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01013999             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14161 16S ribosomal RNA, sequence.
ACCESSION   KHUT01013999
VERSION     KHUT01013999.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14161"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           83 g           56 t
ORIGIN      
        1 tacagaggtt acaagcattg tccggaatca ctgggcgtaa agcgtttcgc aggtgtcttc
       61 ttaagttacg tttgaaagac tggggctcaa ccccagaaac gggcgtaata ctgggaggat
      121 tgaagggtat cggggctatt ggaactgtcg gtgtaggggt gaaatccgtt gatatcgaca
      181 ggaactccga gggcgtaggc agatagctag gtacctcttg acactcatga acgaaagcta
      241 ggggagcgaa aggg
//
LOCUS       KHUT01014000             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14162 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014000
VERSION     KHUT01014000.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14162"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           80 g           55 t
ORIGIN      
        1 tacgtagggt acaagcatta atcggaatta ttgggcgtaa agggtgcgta ggcggaccta
       61 caagtcagat gtgaaatttc ggggctcaac ctcgaagctg catttgaaac tgtaagtctt
      121 gagggttgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagt taataacctg acgctgaggc acgagagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014001             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14163 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014001
VERSION     KHUT01014001.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14163"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctatt
       61 taagtcggat gtgaaagccc ttggctcaac tgaggaagtg cacccgaaac tgaatggctt
      121 gagtaccaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tggatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014002             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14164 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014002
VERSION     KHUT01014002.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14164"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           33 c           81 g           63 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggtgcgta ggtggtaata
       61 taagttagat gtgaaagtcc tgagcttaac ttagggattg cgtttaatac tgtgttactt
      121 gagtattgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga caaatactga cactgaggca caaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014003             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14165 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014003
VERSION     KHUT01014003.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14165"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatta ctgggcgtaa agagctcgta ggcgggggcg
       61 cgcgtctcgg aggaaatttc agggctcaac cctggggctt tccgagatac ggcgcctctt
      121 gaggacagga gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcctga cgctgaggag cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014004             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14166 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014004
VERSION     KHUT01014004.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14166"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           89 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcacgta ggcggcttga
       61 caagtccgat gtgaaatccc ggagctcaac ttcggaactg cattggaaac tgtcaggctt
      121 gagtatagaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggt ctattactga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014005             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14167 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014005
VERSION     KHUT01014005.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14167"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgcgca ggcgggatgg
       61 taagtcggat gtgaaagccc tcggctcaac cgaggaagtg cgttcgaaac tactgttctt
      121 gagtgtcgga gagggtggcg gaattcccgg tgtagaagtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cgaacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014006             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14168 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014006
VERSION     KHUT01014006.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14168"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          100 g           46 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggactta ctgggcgtaa agcgcgcgca ggtggatgag
       61 tatgtgccgt gtgaaagcgc cgtccttacg atggtgaggt catggtagac agcttatcta
      121 gaggctcaga gaggtcgatg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccagg ggcgaaggcg gtcgactggc tgagttctga cactgaggcg cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01014007             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14169 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014007
VERSION     KHUT01014007.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14169"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaatg
       61 taagtcagat gtgaaattcc gaggctcaac ctcggcgctg catctgaaac tgcgtttctt
      121 gagtgtcgga gaggaaagcg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014008             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14170 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014008
VERSION     KHUT01014008.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14170"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 gacgtagggg gcaagcgtta tccggaatga ttgggcgtaa agcgcacgca ggcggttttc
       61 tacgttcagg gtgacagtct ccggcttaac tggggaagtg ccctgaaaac ggggagactt
      121 gagagcgaga gagggacacg gaactccgag tggagcagtg aaatgcgtag agattcggaa
      181 gaacaccaat ggcgaaggct gtgtcctggc tcgtttctga cactcaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014009             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14171 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014009
VERSION     KHUT01014009.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14171"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           54 c           99 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtggggaag
       61 caagtccggt gggaaagccc acggctcaac cgtggaaggt cgctggaaac tgcttttctt
      121 gagggcgaca gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccaa ggcgaaggca gctctctggg ttgcccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014010             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14172 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014010
VERSION     KHUT01014010.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14172"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           78 g           57 t
ORIGIN      
        1 tcccagcagt ccaagtcgca gccacaatta ttgggtctaa aacatccgta gcttgcttta
       61 taagtctctt gtgaaatctg gcctcttaag ggtcgggcgt gcaagagata ctgtttagct
      121 agggaccggg agacgtaaga agtacggacg gggtagcggt aaaatgtgtt gatcccgttt
      181 ggactaacaa tagcgaaggc atcttacgag aacggatccg acagtgaggg atgaaggcta
      241 ggggcgcaaa gtgg
//
LOCUS       KHUT01014011             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14173 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014011
VERSION     KHUT01014011.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14173"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           62 c          104 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttgc
       61 cgcgtccggg gtgaaattcc acggctcaac cgtgaaacgg cctcggatac gggcggcctg
      121 gaggggggta ggggcgggcg gaactctgcg tggagcggtg aaatgcgttg atatgcagag
      181 gaactccggt ggcgaaggcg gcccgctgga ccccatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014012             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14174 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014012
VERSION     KHUT01014012.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14174"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c          100 g           43 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccgc
       61 taagtttggc gtgaaagccc ccggctcacc tggggaacgg cgctggatac tggcgggcta
      121 gagtaccgga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga cggatactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014013             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14175 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014013
VERSION     KHUT01014013.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14175"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           88 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcgg
       61 taagttgggt gtgaaatccc tcggctcaac cgaggaattg cgcccaaaac tgtcgtgctt
      121 gagggagaca gaggtgagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014014             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14176 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014014
VERSION     KHUT01014014.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14176"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           56 c           90 g           40 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggtccgc
       61 caagttcact gcgacagtca ccggctcaac cggtgaagga cggtgaaaac tggcagactt
      121 gagagcgaga gagggacatg gaactccagg tggagcggtg aaatgcgtag agatctggaa
      181 gaacaccgaa ggcgaaggcc gtgtcctggc tcgtatctga cgctcaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01014015             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14177 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014015
VERSION     KHUT01014015.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14177"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          105 g           38 t
ORIGIN      
        1 gacagagggc gcgagcgttg ttcggaatca ttgggcgtaa agcgcgcgca ggcgggtgcg
       61 caagtcggga gtgaaattcc ggggctcaac tccggagcgg cttccgaaac tgcgtgtcta
      121 gggaccggta ggggtcggtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacacctgt ggcgaaggcg gccgactggg ccggatccga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014016             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14178 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014016
VERSION     KHUT01014016.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14178"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcacgta ggcggtttat
       61 taagtcagat gtgaaaactc tcggctcaac tgggagactg catttgaaac tggtaggcta
      121 gagtccagca gagggaagtg gaattccaag tgtagcggtg aaatgcgcag atatttggaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ctgcgactga cgctgaggtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01014017             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14179 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014017
VERSION     KHUT01014017.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14179"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           79 g           57 t
ORIGIN      
        1 tcccagcagt ccaagtcgca gccacatttg ttgggtctaa aacatccgta gcttgcttcg
       61 taagtctttt gtgaaatcgg gcctcttaag ggtccggcgt gcaaaagata ctgcggagct
      121 tgagaccggg agacgtaatg agtacgatga gggtagcggt aaaatgtgtt gatcctcatt
      181 ggactaacaa tagcgaaggc acgttacgag aacggatctg acagtgaggg atgaaggcta
      241 ggggcgcaaa gtgg
//
LOCUS       KHUT01014018             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14180 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014018
VERSION     KHUT01014018.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14180"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c           97 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 caagtcaggg gtgaaatcct ccagctcaac tggagaagtg cctttgatac tggcgggctc
      121 gagtgaggta ggggcgggtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acccgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014019             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14181 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014019
VERSION     KHUT01014019.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14181"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtcgggg gtgaaatact tcggcttaac cggagaacag ctttcgatac tggtggccta
      121 gagggaggta ggggtctgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014020             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14182 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014020
VERSION     KHUT01014020.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14182"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           84 g           57 t
ORIGIN      
        1 tacgtaagtg gcgagcgtta tccggaatta ctgggtgtaa agggagtgca ggcggccttt
       61 taagtctcat gttaaatttc ccggcttaac cgggaacctg cgtgagaaac tggaaggctt
      121 gagtagagga gaggaagctg gaattcctag tgtagcggtg aaatgtgtag atattaggaa
      181 gaacaccagt ggcgaaggcg ggcttctggc cttttactga cgctgatact cgaaagccgg
      241 gggagcaaac agg
//
LOCUS       KHUT01014021             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14183 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014021
VERSION     KHUT01014021.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14183"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           47 c           94 g           52 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggccgtt
       61 taagtttggt gtgaaatctc ccggctcaac tgggagggtg cgccgaatac tgaatggctc
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgtaactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014022             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14184 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014022
VERSION     KHUT01014022.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14184"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           90 g           51 t
ORIGIN      
        1 gacgaaggtg gctagcgttg ttcggaatca ctgggcttaa agcgcatgta ggcgtcctgt
       61 taagtgcctt gtgaaatccc tcggctcaac cgaggaactg cttggcatac tggcaggatt
      121 gaggcaggta tgggtgcatg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacgccgga ggtgaagacg atgcactggg cctgtcctga cgctgagatg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014023             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14185 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014023
VERSION     KHUT01014023.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14185"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           90 g           50 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgctctg
       61 tgtgtcggat gtgaaagccc tgggctcaac ctaggaagtg cattcgaaac tacagagctt
      121 gagtcctgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccttctgga cagtgactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014024             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14186 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014024
VERSION     KHUT01014024.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14186"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           81 g           57 t
ORIGIN      
        1 tacagaggtg gcgagcgtta ctcggattta ttgggtgtaa agggcaagta ggcgtcttaa
       61 caagttagga gtgaaatcct gcagctcaac tgcagaactg ctcttaaaac tgttgagatt
      121 gagtttggga gaggaaagcg gaattctcgg tgtaagagtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg gctttctggc ccagtactga cgctgaattg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014025             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14187 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014025
VERSION     KHUT01014025.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14187"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           44 c           93 g           54 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtgctt
       61 caagtctgtt gtgaaatctc ccggcttaac tgggagggtg cggtggaaac tgaagcgctt
      121 gagtatggga gaggtaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gcttactgga ccatgactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014026             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14188 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014026
VERSION     KHUT01014026.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14188"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           99 g           46 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agcgcacgca ggcggcttcg
       61 tgagtcggac gttaaagctc ctggcttaac tgggagaggc cgttcgatac tgcggggctt
      121 gaggttggga gaggggtgcg gaattccggg tgtagtggtg gaatgcgtag atatccggag
      181 gaacacctgt ggcgaaggcg gcaccctggc ccacacctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014027             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14189 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014027
VERSION     KHUT01014027.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14189"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           53 c           89 g           40 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggcgcgg
       61 taagtcggct gtgaaagccc tcggctcaac cgaggaagtg cagccgaaac taccaagcta
      121 gagtatcgaa gagggtggcg gaatgcgcag tgtagaggtg aaattcgtag atattgcgta
      181 gaacaccagt ggcgaaggcg gccacctggg agactactga cgctaagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014028             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14190 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014028
VERSION     KHUT01014028.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14190"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           96 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggaatg
       61 tacgtccgtc gctgaaatcc cccggctcaa ccggggaagt ggcgcggata cggcattcct
      121 tgaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcctactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014029             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14191 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014029
VERSION     KHUT01014029.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14191"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           60 c           97 g           42 t
ORIGIN      
        1 cacgtagggg acgagcgttg tccggattca ttgggcgtaa agcgcgcgta ggcggcccgg
       61 aaagtcggtc gtgaaatcct ggggctcaac cccatgactg cgtccgatac tcccgggctg
      121 gaggcaggta ggggagatcg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccttgcctga cgctgaggcg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01014030             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14192 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014030
VERSION     KHUT01014030.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14192"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           52 c           86 g           47 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggtccgt
       61 taagttgggt gtgaaagacc tcggctcaac cgaggaatgg cgcccaaaac tagcggactt
      121 gagtaaggca gaggtgaacg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt agcgaaagcg gttcactggg ccttttctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014031             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14193 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014031
VERSION     KHUT01014031.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14193"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           41 c           92 g           57 t
ORIGIN      
        1 tacagaggtg gcgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggttttg
       61 tgtgtcggat gtgaaatctc actgctcaac ggtgaaatgg cattcgaaac tgcagaactt
      121 gagtgcagga gaggagagcg gaattcttgg tgtagcggtg gaatgcgttg agatcaagaa
      181 gaacaccggt ggcgtaggcg gctctctggt atgcaactga cactgaggca cgaaagttgg
      241 gggagcaaac agg
//
LOCUS       KHUT01014032             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14194 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014032
VERSION     KHUT01014032.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14194"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           91 g           49 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcatgta ggtggcttga
       61 aaagttggac gtgaaagctc ccggcttaac tgggagaggt cgttcaaaac tgacaagcta
      121 gaggttggaa gaggagagcg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tcaatactga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014033             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14195 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014033
VERSION     KHUT01014033.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14195"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           93 g           44 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgaa
       61 agggtgtctt gtgaaatccc ccggctcaac cggggaactg cgaggcaaac cgttcgactt
      121 gaggcagtta gaggtcgatg gaactctggg tggagcggtg aaatgcgtag atatccaggg
      181 gaacgccaat ggtgaaaaca gtcgactggg cctgtcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014034             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14196 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014034
VERSION     KHUT01014034.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14196"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           80 g           57 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtctttt gttaaaaccc agggcttaac cctggatctg cgattgatac ggcacaacta
      121 gaggatgcga gagatgtatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gtacattggc gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014035             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14197 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014035
VERSION     KHUT01014035.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14197"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           98 g           41 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 caagtctggg gtgaaatcct ccggctcaac cggagaacgg cctcggatac tggcggcctc
      121 gagggggata ggggcgagcg gaactgtgag tggagcggtg aaatgcgttg atattcacag
      181 gaactccggt ggcgaaggcg gctcgctggg tctcatctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014036             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14198 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014036
VERSION     KHUT01014036.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14198"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggatctg
       61 acagtcagat gtgaaattcc tgggctcaac ctgggggctg catttgatac gtgaggtctt
      121 gagtccggaa gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccacctggt ccggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014037             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14199 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014037
VERSION     KHUT01014037.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14199"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           95 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cacgtcgact gtgaaaaccc atggcttaac tgtgggcttg cagtcgatac gggctggcta
      121 gagtacggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgttactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014038             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14200 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014038
VERSION     KHUT01014038.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14200"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           38 c           78 g           64 t
ORIGIN      
        1 tacgtaggtc tcaagcgtta tccggattta ctgggcgtaa agagtctgta gctggttgca
       61 taagtctatt tttaaagacc ttggctcaac taagggaagg gaatggatac tgtgcatctg
      121 gaggttatta ggggttagta gaattcccgg tggaggggtg aaatccgttg atattgggag
      181 gaatgccgaa agcgaaagca gctaactata attatcctga cagtgagaga cgaaagcatg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014039             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14201 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014039
VERSION     KHUT01014039.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14201"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           92 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcgggttag
       61 caagtcgatt gtgaaagccc cgggcttaac ctgggaattg cagtcgaaac tgctgatctt
      121 gaatgcccga gaggatggcg gaattcccgg tgtagaagtg aaattcgtag atatcgggaa
      181 gaacacctga ggcgaaggcg gctatctggt ggtgcattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014040             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14202 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014040
VERSION     KHUT01014040.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14202"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttatt
       61 taagtctggt gttaaagtcc ggagctcaac tccgggttcg cattggaaac tgaatagctt
      121 gagtgcggta gaggtaaagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ctttactgga ccgcaactga cgctgaggaa cgaaagccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01014041             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14203 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014041
VERSION     KHUT01014041.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14203"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           89 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggtggatcgc
       61 taagtctggt gtgaaatccc tcggcttaac tgaggacgtg cattggatac tggcgacctt
      121 gagtcctgga gaggatagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctatctgga cagtgactga cactgagacg cgaaagcacg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014042             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14204 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014042
VERSION     KHUT01014042.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14204"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           90 g           52 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggcttgg
       61 caagtccgat gtgaaagccc agggctcaac cctgggattg cattggaaac tgttaggcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014043             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14205 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014043
VERSION     KHUT01014043.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14205"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c           96 g           47 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggcctgt
       61 taagtctgct gtaaaatctc ccggcttaac cgggaggagc cagcggatac tggcaggctt
      121 gaggcaagca gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg cttgtcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014044             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14206 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014044
VERSION     KHUT01014044.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14206"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           93 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ttgggcgtaa agggctcgta ggcgggctcg
       61 tatgtcgatt gtgaaagccc caggctcaac ctgggaattg cagtcgaaac tgcgagtctt
      121 gaatacctga gagggtggga gaattcctgg tggagtagtg aaatacgtag agatcaggag
      181 gaataccaga ggcgaaggcg cctgcctggc agtgtattga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014045             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14207 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014045
VERSION     KHUT01014045.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14207"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           35 c           76 g           66 t
ORIGIN      
        1 cacggaaggt gcaagcgtta tccggtttta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtt gtgaaatgct aacgcttaac gttagagctg caattgaaac tggtaggctt
      121 gagttaagaa gaggtaagta gaatttatgg tgtagtagtg aaatgcatag atatcataag
      181 gaataccaat ggcgtaggca gcttactggt ctataactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014046             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14208 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014046
VERSION     KHUT01014046.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14208"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           92 g           42 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtccgt
       61 taagtcgggt gtgaaatctc caagctcaac ttggaaactg cacccgatac tggcggactt
      121 gaggacggta gaggtcggtg gaatgccgag tgtaccggtg aaatggacag atattcggcg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01014047             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14209 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014047
VERSION     KHUT01014047.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14209"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           97 g           40 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccga
       61 taagtcccgc gtgaaagccc ccggctcaac tggggaattg cgcgggaaac tattgggctt
      121 gagatcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014048             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14210 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014048
VERSION     KHUT01014048.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14210"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           77 g           64 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ttgggtttaa agggtgcgta ggtggataag
       61 taagtcagtg gtgaaagcct gcagcttaac tgtagaattg ccattgatac tgcttgtctt
      121 gagtacattt gaggtgggcg gaatatgaca tgtagcggtg aaatgcttag atatgtcata
      181 gaacaccgat tgcgaaggca gctcactaaa ctgttactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014049             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14211 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014049
VERSION     KHUT01014049.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14211"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           87 g           51 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggccata
       61 caagtcgggt gtgaaatccc ggagcttaac tccggaatgg cattcgaaac tgtatggctc
      121 gagatctgga gaggtgattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaggcg aatcactgga cagtatctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014050             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14212 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014050
VERSION     KHUT01014050.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14212"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           89 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggtg
       61 taagtcggat gtgaaatccc ggggctcaac cctggaactg cattcgatac tgcatcgctt
      121 gagtactgga gagggaactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg agttcctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014051             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14213 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014051
VERSION     KHUT01014051.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14213"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c          100 g           51 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgacc gtgaaatctt ggggcttaac cctgagtctg cggttgatac gggcaggcta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014052             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14214 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014052
VERSION     KHUT01014052.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14214"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           93 g           48 t
ORIGIN      
        1 tacggagagt gcaagcgttg ttcggaatca ttgggcgtaa agagcgtgta ggcggcctgc
       61 taagtctggt gtgaaagccc tcggcttaac cgaggaagtg cattggatac tggcaggctt
      121 gagtacggga gagggaagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014053             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14215 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014053
VERSION     KHUT01014053.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14215"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 tacggaggtt ccaagcgttg ttcggaatta ctgggcgtaa agggggcgca ggcggactgg
       61 tcagtggaaa gtggaagccc ggggctcaac ctcggaggtg ctttccatac tgccagtctt
      121 gagttcgtga gaggagagcg gaattcaggg tgtagcggtg aaatgcttag atatcctgag
      181 gaacaccggt ggcgaaagcg gctctctggc acgaaactga cgctgaggcc cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014054             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14216 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014054
VERSION     KHUT01014054.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14216"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           87 g           49 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggtccgca ggtggcattg
       61 taagtctgct gttaaagagt ctagctcaac tagataaaag cagtggaaac tacaaagcta
      121 gagtatggtc ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacatcggt ggcgaaggcg ctctgctagg ccaaaactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01014055             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14217 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014055
VERSION     KHUT01014055.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14217"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           88 g           51 t
ORIGIN      
        1 tacggagtgt gctagcgtta ctcggaatca ctgggcataa agcgcgcgta ggcggagtct
       61 caagtcggtt gtgaaatccc tcagcttaac tgaggagctg cagccgatac tgtgactctt
      121 gaggacgaaa ggggagagcg gaacttctgg tgaagcggtg aaatgcgtag atatcagaag
      181 gaacgccggt ggcgaaagcg gctctctggt tcgtatctga cgctgaggcg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014056             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14218 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014056
VERSION     KHUT01014056.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14218"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           99 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtcagt
       61 tgcgtcgttg gtgacagtcc ccggcttaac tggggaagcg ctggcgaaac gggttgactt
      121 gagggccaga gagggacacg gaactccggg tggagcggtg aaatgcgtag agatccggaa
      181 gaacaccgaa ggcgaaggca gtgtcctggc tggttcctga cgctcaggtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014057             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14219 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014057
VERSION     KHUT01014057.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14219"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           90 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggtggttggg
       61 taagtccgca atgaaatctc ccggcttaac tgggaggggt ttgtggatac tgtccggctg
      121 gagtgtggga gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacctaa ggcgaaagca ggttactggc ccaatactga cactgagctg cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01014058             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14220 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014058
VERSION     KHUT01014058.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14220"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           43 c           96 g           55 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggtcctt
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tgaggggcta
      121 gagtgcggga gaggatagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctatctgga ccgtgactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014059             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14221 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014059
VERSION     KHUT01014059.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14221"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           80 g           58 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggttttt
       61 taagttagag gtgaaatccc ggtgctcaac accggaactg cctctgatac tggaaggcta
      121 gagagtagat gctgtgggcg gaatgtatgg tgtagcggtg aaatgcttag agatcataca
      181 gaacaccgat tgcgaaggca gctcacaaat ctatatctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014060             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14222 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014060
VERSION     KHUT01014060.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14222"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           86 g           58 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatatt
       61 taagtgagat gtgaaattcc taggcttaac ttgggagctg catttcaaac tgggtatcta
      121 gagtgcggga gaggagaatg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg attctctgga ccgtaactga cgctgaggca tgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014061             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14223 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014061
VERSION     KHUT01014061.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14223"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           57 c          101 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggactta ctgggcgtaa agggcgcgta ggcggccagg
       61 tacgtttcgg gtgaaatcgc cccgcttaac ggggcgcggt ctcggaatac ggcttggcta
      121 gaggcatgca gagggtggtg gaattcccgg cgtagtggtg aaatgcgtag agttcgggag
      181 gaacaccagt ggtgcagacg gccacctggg catgacctga cgctgaggcg cgatagcgtg
      241 gggagcgaac cga
//
LOCUS       KHUT01014062             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14224 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014062
VERSION     KHUT01014062.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14224"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccgaattca ctgggcgtaa agcgcgcgta ggcggatttt
       61 taagttggag gtgaaatccc ggggctcaac cccggaactg cccccaaaac tggaagtctt
      121 gaggtatgga gagggaagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacacccgt ggcgaaggcg gcttcctggt catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014063             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14225 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014063
VERSION     KHUT01014063.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14225"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgtggggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcccgt
       61 taagtctgat gtgaaatccc tgggcttaac ccaggaactg catcggaaac taacaggctg
      121 gagtccggga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga ccggtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014064             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14226 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014064
VERSION     KHUT01014064.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14226"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           49 c          103 g           49 t
ORIGIN      
        1 gacgtagggc gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttgt
       61 tgcgtcgatc gtgaaaacct gcggcttaac tgtgggcgtg cggtcgatac gggcaggctg
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014065             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14227 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014065
VERSION     KHUT01014065.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14227"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           92 g           48 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttgt
       61 ctagtccggg gtgaaatccc acagcttaac tgtggaactg cctcggatac tggcagtctc
      121 gagggtggta ggggtatgcg gaactatcgg tggagcggtg aaatgcgttg atatcgatag
      181 gaactccggt ggcgaaggcg gcatactgga ccacatctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014066             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14228 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014066
VERSION     KHUT01014066.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14228"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggctgtgtcg
       61 caagtcaggg gtgaaatccc ggagcttaac tccggaactg cctttgaaac tgcgatgctg
      121 gaatctcaga ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga tgagtattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014067             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14229 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014067
VERSION     KHUT01014067.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14229"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           85 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgca ggtggctgga
       61 aaagtttgat gtgaaatctc cgggcttaac ccggaaatgg cattgaatac tcttcagctg
      121 gaggtttgga ggggggactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014068             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14230 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014068
VERSION     KHUT01014068.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14230"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           98 g           47 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggcgtaa agggccagta ggtggttcga
       61 tgcgttgggt ttgaaatcac ctggcttaac tgggtgaatg ggcccaaaac ggtcggactg
      121 gaggtaggga gaggggtgca gaattcctgg tgtagtggtg gaatgcgtag agatcaggag
      181 gaatacccgt ggcgaaagcg gcaccctggc cctgacctga cactgagggg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014069             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14231 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014069
VERSION     KHUT01014069.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14231"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          104 g           36 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcaggg
       61 tacgcgtgcc gtgaaagccc ggggctcaac cccgggtcgg cggtgcgaac ggctcagctt
      121 gagcatacga gaggcaggcg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcctgctggc gtatagctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014070             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14232 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014070
VERSION     KHUT01014070.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14232"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           63 c          103 g           33 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggactga ctgggcgtaa agcgcccgca ggcggtcgcg
       61 tcagtgccgt gtgaaagccg gcggcttaac tgccggaggc catggcagac cgcgcgactg
      121 gagcgatgca gagggtggtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggggaaccca gccacctggg catcggctga cgctgagggg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014071             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14233 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014071
VERSION     KHUT01014071.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14233"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           86 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggaatat
       61 taagtcagat gtgaaactcc agggctcaac tctggaactg catttgaaac tgatattcta
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg agatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014072             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14234 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014072
VERSION     KHUT01014072.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14234"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacgaactgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttcgc
       61 caagtcaggt gtgaaatccc ccggctcaac cggggaacag cgcttgaaac tagcgggctg
      121 gagcgaggca ggggtgtgtg gaacttccgg tggagcggtg aaatgtgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcacactggg ccttgactga cgctgaggca cgaaagccag
      241 gggagtgaac ggg
//
LOCUS       KHUT01014073             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14235 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014073
VERSION     KHUT01014073.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14235"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           87 g           56 t
ORIGIN      
        1 tacgaggggt cctagcgttg ttcggaatta ctgggcgtaa agcgtatgta ggtggcttgg
       61 caagtcaggt gtgaaagcct cgggcttaac ccgagaagtg cacttgatac tgctgagctt
      121 gagtatggga gagggtagta gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaataccggt ggcgaaggcg gctacctggc ccaatactga cactgagata cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014074             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14236 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014074
VERSION     KHUT01014074.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14236"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           49 a           59 c          113 g           32 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggcccgt
       61 taagtctgcg gtgaaagccc ggggctcaac cccggagggt ccgcggagac tggggggcta
      121 gggggtcgga gaggagcggg gaatcccggg tgtagcggtg gaatgcgtag agatccgggg
      181 gaacaccgat ggcgaaggca ccgctctggc cgacgaccga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014075             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14237 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014075
VERSION     KHUT01014075.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14237"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           87 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgca ggcgggaagg
       61 ttagttcgtg gtgaaagacg atcgctcaac gattgcagtg ccatgaatac tgcctttctt
      121 gaatctggtt gaggccggtg gaattgaccg tgtagcggtg aaatgcatag atatggtcaa
      181 gaactccaat tgcgaaggca gccggctggg ccagcattga cgctcaggca cgagagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01014076             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14238 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014076
VERSION     KHUT01014076.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14238"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           80 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agagttcgta ggtggactta
       61 ttagtatagg gttaaatacc ggggcctaac tccggacctg ctctgtaaac ggtaagtcta
      121 gagtactaga gaggatagtg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacatctga ggcgaaggcg actatctggc taggcactga cactaaagaa cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014077             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14239 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014077
VERSION     KHUT01014077.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14239"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           96 g           38 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcc
       61 caagtcggtg gtgaaatcgt ccggcccaac cggacaagtg ccgccgatac tgggggcctc
      121 gagggaggta ggggtcgagg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014078             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14240 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014078
VERSION     KHUT01014078.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14240"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           90 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggtcacc
       61 ccagtcggat gtgaaagccc agggctcaac cctggacgtg catacgatac taggtggctg
      121 gagtctcgga gaggatagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgagtactga cactgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014079             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14241 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014079
VERSION     KHUT01014079.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14241"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           89 g           56 t
ORIGIN      
        1 tacgtaggtg gcgagcgtta tccggattta ctgggtgtaa agggtgtgta ggcgggattg
       61 caagtcggat gtgaaaacta tgggctcaac tcatagcttg cattcgaaac tgtagttctt
      121 gagggtcgga gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg agttactgga cgatacctga cgctgagaca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014080             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14242 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014080
VERSION     KHUT01014080.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14242"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           53 c           89 g           45 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcccgg
       61 gaagttgggt gtgaaagccc tcggctcaac cgaggaattg cgcccaaaac tgccgggctt
      121 gaggaagata gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt agcgaaagcg gctcactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaag agg
//
LOCUS       KHUT01014081             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14243 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014081
VERSION     KHUT01014081.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14243"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c          101 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggcctgg
       61 taagtagggg gtgaaatctc acggctcaac cgtgaggctg cctcttagac tgcctggctc
      121 gagcatggta gaggcgagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaggcg gctcgctggg ccattgctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014082             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14244 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014082
VERSION     KHUT01014082.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14244"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           61 c          102 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agggcgcgta ggctggctgc
       61 cgcgtcgggg ctgaaagccc ccggcttaac cggggaatgg gcctcgagac gggcagcctg
      121 gaggcgagga gggggacctg gaacgtccgg tggagcggtg aaatgcgttg agatcggacg
      181 gaactccgac ggcgaaagca aggtcctgga ctcgtactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014083             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14245 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014083
VERSION     KHUT01014083.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14245"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           94 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaattta ctgggcgtaa agcgcgcgta ggcggacctt
       61 taagtcaggg gtgaaattcc ggggctcaac tccggaactg cctttgaaac tggaggtctt
      121 gaggtatgga gagggaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gcttcctggc catatcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014084             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14246 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014084
VERSION     KHUT01014084.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14246"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggctgccttg
       61 taagtcggat gtgaaagccc cgggctcaac ccgggaagtg cacccgatac tacaaggctg
      121 gagtctcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgatgactga cgctgagaca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014085             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14247 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014085
VERSION     KHUT01014085.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14247"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           82 g           51 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgcgca ggcggattgt
       61 taagtaggga gtgaaaggtt ggggctcaac cctaacactg ctccctatac tggcagtctt
      121 gagtggcgga gaggaagatg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca atcttctgga cgcaaactga cgctcatgca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01014086             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14248 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014086
VERSION     KHUT01014086.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14248"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           98 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcacgca ggcggtgctg
       61 caagtttggg gtgacagctc ctcgcttaac gaggagagcg tctcgaagac tgcaggactt
      121 gagggccaga gagggacaca gaattccggg tggagtggtg aaatgcgtag atatccggag
      181 gaataccaaa ggcgaaggca gtgtcctggc tggtacctga cgctcaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014087             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14249 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014087
VERSION     KHUT01014087.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14249"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 tacggagggg gcgagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggcggctttg
       61 taagtcccgg gtgaaagccc tcggctcaac cgaggaactg ccttggatac tgcaaagctt
      121 gaggctggaa gagggcagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctgcctggg ccagttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014088             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14250 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014088
VERSION     KHUT01014088.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14250"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 taagtcggac gtgaaagctc ggggcttaac cccgaaatgg cgttggaaac taccttgcta
      121 gaggattgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014089             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14251 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014089
VERSION     KHUT01014089.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14251"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           95 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggcttgt
       61 cgcgtctgct gtgaaatctg gtggctcaac catcagcgtg cagtgggtac gggcaagcta
      121 gagtgcggta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gatctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01014090             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14252 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014090
VERSION     KHUT01014090.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14252"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           84 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agagcacgta ggcggattag
       61 aaagttggat gtgaaagccc cgggctcaac ctgggaactg cattcaaaac tactagtcta
      121 gagtatagga gaggaaagcg gaatttccgg tgtagcagtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg gctttctggc ctaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014091             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14253 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014091
VERSION     KHUT01014091.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14253"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggt
       61 caagtcaggg gtgaaatccc acggctcaac cgtggaagtg cctttgatac tggccgtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014092             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14254 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014092
VERSION     KHUT01014092.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14254"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           65 c          107 g           31 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgc
       61 ggcgtccggg gtgaaatcct cccgcccaac gggagaacgg cctcggatac gggcggcctg
      121 gaggggggta ggggcgggcg gaactgtggg tggagcggtg aaatgcgttg agatccacag
      181 gaactccggt ggcgaaggcg gcccgctgga ccccacctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014093             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14255 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014093
VERSION     KHUT01014093.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14255"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggtggt
       61 caagtcagag gtgaaatcct ccagcttaac tggagaagag cccctgatac tggccatctc
      121 gagggaggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014094             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14256 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014094
VERSION     KHUT01014094.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14256"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           91 g           53 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggttcgt
       61 taagtgcaca agtgaaagct ccaggctcaa cttggaaatt gctttgcata ctggcggact
      121 tgagggacgc agaggtctgt ggaattcctg gtgtaacggt gaaatgtgtt gatatcagga
      181 ggaacaccca tggcgaaggc agcagactgg gcgtttcctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01014095             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14257 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014095
VERSION     KHUT01014095.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14257"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggttttg
       61 taagtctgat gtgaaagccc cgggctcaac ctgggaatgg cattggagac tgcaagactg
      121 gaatctggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag atatgtggag
      181 gaacaccgat ggcgaaggca gccccctggg tcaagattga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014096             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14258 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014096
VERSION     KHUT01014096.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14258"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           90 g           41 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggtcggc
       61 caagttcact gcgacagtcc ccggcttaac cggggaagga cgatgaacac tggccgactt
      121 gagagcgaga gagggacatg gaactccagg tggagcggtg aaatgcgtag agatctggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggc tcgtatctga cgctgatgtg cgaaagctag
      241 gggagcgaac agg
//
LOCUS       KHUT01014097             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14259 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014097
VERSION     KHUT01014097.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14259"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgtgta ggcgggcaat
       61 taagtcagcg gtgaaatctt caggctcaac ctgaaaactg ccattgatac tatttgtctt
      121 gaatatcgtt gaggtaggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gcttgctaaa cgatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014098             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14260 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014098
VERSION     KHUT01014098.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14260"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           38 c           89 g           57 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggatat
       61 taagtcagat gtgaaagtcc taggctcaac ttagggagtg catctgaaac taatgttctt
      121 gagtggagga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg actttctgga ctttaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014099             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14261 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014099
VERSION     KHUT01014099.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14261"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           88 g           53 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgcatgta ggcggccaaa
       61 taagtcaggt gtgaaagccc tgggctcaac ccgggaagtg cacttgatac tgcttggctt
      121 gaatatggga gaggatagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctggc ccaatattga cgctgagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014100             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14262 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014100
VERSION     KHUT01014100.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14262"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtt
       61 caagtcgagt gtgaaagccc ccggctcaac tggggagggt cactcgatac tgatcgactc
      121 gaaggcagaa aaaaaaagtg gaattcccgg tatagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg acttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014101             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14263 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014101
VERSION     KHUT01014101.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14263"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          101 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaagct
       61 taagtgtggc ctgaaagccc ccggctcaac cggggagtgc ggctgcaaac tgggtttctt
      121 gagggcggca gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gctctctggg ccggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014102             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14264 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014102
VERSION     KHUT01014102.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14264"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           92 g           45 t
ORIGIN      
        1 tacagggggt gcaagcgttg ctcggaatca ctgggcgtaa agcgagcgca ggcggaccgt
       61 taagtcgggt gtgaaatccc ggggctcaac cccggaactg cattcgaaac tggcggtctt
      121 gagtacggga gaggtgagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctcactggc ccgatactga cgctcatgct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014103             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14265 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014103
VERSION     KHUT01014103.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14265"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           92 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttcac
       61 taagtctgct gtgaaatcta cgggcttagc ctgtaaagtg cagcggaaac tggtggactt
      121 gagtatcgga gagggcaggg gaatacccag tgtagaggtg aaattcgtag agattgggtg
      181 gaacaccagt ggcgaaggcg cctgcctggc tgatgactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014104             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14266 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014104
VERSION     KHUT01014104.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14266"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           95 g           47 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgtcg
       61 caagtccgtg gtgaaatctc tgggctcaac tcagagtctg ccacggaaac tgcggtgcta
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014105             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14267 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014105
VERSION     KHUT01014105.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14267"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           96 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgtgta ggcggtcctc
       61 taagttcggt gtgaaatctc ccggcttaac cgggagggtg cgccggaaac tggggggctc
      121 gagtgtggga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgtagacg gctttctgga ccattactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014106             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14268 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014106
VERSION     KHUT01014106.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14268"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           97 g           40 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtaa agcgcacgca ggcggtacgg
       61 caagtggtgt gtgaaagcgg tcggcttaac tgaccgaggc catgccaaac tgccgcactc
      121 gagcactgca gaggtcgatg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccaag ggggaaacca gtcgactggg caggtgctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac tgg
//
LOCUS       KHUT01014107             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14269 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014107
VERSION     KHUT01014107.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14269"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtcatt
       61 taagtagggt gtgaaagccc tcggctcaac cggggaactg cgtcctaaac tgggtgactg
      121 gagtgagata gaggtgtgtg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014108             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14270 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014108
VERSION     KHUT01014108.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14270"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          106 g           39 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggcttga
       61 tgcgtcacac gtgaaagccc ctggctcaac cagggagggt cgtgtgaaac ggtcaagctg
      121 gaggcagcga gaggctggtg gaattcccgg agtagtggtg aaatgcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gccagctggc gctgacctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014109             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14271 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014109
VERSION     KHUT01014109.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14271"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgtaggtt gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcggagatg
       61 caagttggga gtgaaatcca tgggctcaac ccatgaactg ctttcaaaac tgtatccctt
      121 gagtatcgga gaggcaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttgctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014110             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14272 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014110
VERSION     KHUT01014110.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14272"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           87 g           54 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agagggcgta ggtggccttg
       61 taagtcagat gtgaaagccc agggctcaac cctggaagtg catttgatac tgcttggctt
      121 gaatgcgaga gaggtaagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gcttactggc tcgacattga cactgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014111             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14273 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014111
VERSION     KHUT01014111.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14273"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcggcg
       61 taagtcggtg gtgaaatccc tcagctcaac tgaggaagtg ccctcgatac tgcgtcgctt
      121 gaatgtcgga ggggatggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccatctgga cgacaattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014112             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14274 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014112
VERSION     KHUT01014112.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14274"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           83 g           54 t
ORIGIN      
        1 tacgaaggtg acaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggtcggtttg
       61 taagtcgatt gtgaaaaccc tgggcttaac ccgggacgtg cagtcgaaac tgcaaatctt
      121 gaatgctcga gaggatagta gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaataccgga ggcgaaagcg gctatctggc gagtcattga cactgaggtg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01014113             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14275 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014113
VERSION     KHUT01014113.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14275"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggtttcg
       61 taagtccgac gtgaaagtcc agggctcaac cctggaatgc cgttggatac tgcggaacta
      121 gagtccggaa ggggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014114             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14276 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014114
VERSION     KHUT01014114.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14276"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           46 c           68 g           59 t
ORIGIN      
        1 tacaaaaaat gcaagcgtta tccggattca ctaggtttaa agggtacgta ggtaggcagg
       61 taagtcagtg gtgaaatctc caggcttaac tcggaaactg ccattgatac tatctgtctt
      121 gaataccgtg gaggtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctggctaca cgaatattga cactgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014115             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14277 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014115
VERSION     KHUT01014115.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14277"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           89 g           53 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggtctat
       61 taagtcgggt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac taatagacta
      121 gaaggtggta gaggatggtg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaataccggt ggcgaaggcg gccatctggg ccactcttga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014116             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14278 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014116
VERSION     KHUT01014116.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14278"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           91 g           48 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaagta ggtggtctca
       61 tttgtctggg gtgaaagcct tgagcttaac tcaagaagtg ccccggaaac ggtgagactc
      121 gagtattgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caattactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014117             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14279 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014117
VERSION     KHUT01014117.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14279"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           78 g           56 t
ORIGIN      
        1 tacataggtc acaagcgtta tccggaatta ttgggcgtaa agggtttgta ggtggtaaag
       61 cgggtcttcg gtaaaatccc caagcttaac ttgggtttcg ccgaagaaac tactttgctc
      121 gagggtagca ggggcatacg gaatacccgg tgtagcagtg aaatgcgttg atatcgggta
      181 gaacaccaat ggcgcaagca gtatgctagg ctactcctga cactgaaaaa cgaaagcgtg
      241 gggagcgaat ggg
//
LOCUS       KHUT01014118             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14280 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014118
VERSION     KHUT01014118.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14280"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           96 g           49 t
ORIGIN      
        1 tacgaggggg gcgagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 tatgtctcct gtgaaaactc caggctcaac ttggagctgg caggggaaac tgccgtgctt
      121 gagtactgga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga cagtaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014119             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14281 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014119
VERSION     KHUT01014119.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14281"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggcggcg
       61 taagtacctt gtgaaatccc acggctcaac cgtggaactg ctcggtatac tgcgtcgctt
      121 gagccagcta ggggcaaccg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccgat ggtgaaaaca ggttgctggg gctgcgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014120             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14282 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014120
VERSION     KHUT01014120.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14282"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgcag
       61 caagtcaggg gtgaaatccc agagcttaac tctggaactg cctttgaaac tgctgtgctg
      121 gaatctcaga ggggatagcg gaattccaaa tgtaggggtg aaatccgtag atatttggag
      181 gaacaccggt ggcgaaggcg gctatctgga tgagtattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014121             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14283 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014121
VERSION     KHUT01014121.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14283"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           93 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 caagtcggat gtgaaatccc tcggctcaac cggggaactg cattcgaaac tgcttggctt
      121 gagtactgga gagggtggcg gaattcccgg tgtaggagtg aaatccgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccacctgga cagtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014122             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14284 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014122
VERSION     KHUT01014122.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14284"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           89 g           53 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgcatgta ggcggcctag
       61 caagtcaggt gtgaaagccc agggcttaac cctggaagtg catttgatac tgctgggctt
      121 gaatatggga gaggatagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctggc ccaatattga cgctgagatg cgaaagcgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01014123             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14285 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014123
VERSION     KHUT01014123.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14285"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           93 g           45 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctcgt
       61 taggtagggt gtgaaagccc ccggctcaac cggggaattg cgccctaaac cggcgggcta
      121 gagtgagata ggggtgtgtg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg acacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014124             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14286 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014124
VERSION     KHUT01014124.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14286"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           88 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgtta tccgaattta ctgggcgtaa agcgcgtgta ggcggctcct
       61 taagtgagat gtgaaatctt gcggctcaac cgcaaaactg catttcaaac tggggggctt
      121 gaatttggga gaggaaagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctttctggc ccaacattga cgctgagacg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014125             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14287 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014125
VERSION     KHUT01014125.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14287"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           53 c           84 g           46 t
ORIGIN      
        1 tacgaaccgt ccaaacgtta ttcggaatca ctgggcctaa agggtgcgta ggcggctcag
       61 aaagtagggt gtgaaatacc tcggctcaac cgaggaattg cgccctaaac tactgggctc
      121 gaggaagaca gaggtaagcg gaacttacgg tggagcggtg aaatgcgttg atatcgtaag
      181 gaacaccagg agcgaaagcg gcttactggg tcttttctga cgctgaggca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014126             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14288 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014126
VERSION     KHUT01014126.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14288"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           86 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa aggatgcgta ggcggatttt
       61 taagtcagat gtgaaaaccc cgagctcaac ttggggactg catttgaaac tggaagtcta
      121 gagtgcggga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actttctgga ccgtaactga cgctgaggca tgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014127             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14289 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014127
VERSION     KHUT01014127.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14289"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           42 c           72 g           62 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agagcatgta ggcggacata
       61 ttagtcagac gtcaaatctt cgagctcaac tcgaaaactg cgtttgaaac ggtatgtctt
      121 gagaaagtga gagatcaatg gaacatatgg tgtagcagtg aaatgcgttg atatcatatg
      181 gaacaccaaa ggcgaaggca tttgattggc acttttctga cgctgagatg cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014128             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14290 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014128
VERSION     KHUT01014128.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14290"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           89 g           46 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggttggt
       61 caagttggat gtgaaatctc ccggctcaac tgggagagga cgttcaaaac tggccgactt
      121 gaggttggta gagggaggcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctagg ccatacctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014129             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14291 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014129
VERSION     KHUT01014129.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14291"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          101 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agagcgcgta ggcgggttgg
       61 caagtccgct gtgaaagccc ggggcttaac cccggagggt cggtggatac tgccagtctt
      121 gaagatacta ggggaagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacacccgt ggcgaaggcg gtcttctggg gtagtcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014130             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14292 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014130
VERSION     KHUT01014130.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14292"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           87 g           51 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcgga
       61 caagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgttcgtctt
      121 gaggcatcta ggggcaactg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca ggttgctggg gatgttctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014131             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14293 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014131
VERSION     KHUT01014131.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14293"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           76 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggactga
       61 taagtcagtg gtgaaatctt ctcgctcaac gggaaaactg ccattgatac tatttgtctt
      121 gaattcggtt gaggtgagtg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctcactaag ccgaaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014132             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14294 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014132
VERSION     KHUT01014132.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14294"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          101 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agcgcgtgta ggcggagcgt
       61 tcagtctggc ctgaaagccc ccggctcaac cggggaaggt ggctggaaac tggcgttctt
      121 gagggcgata gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctggg tcggccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014133             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14295 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014133
VERSION     KHUT01014133.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14295"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           77 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggcagg
       61 taagtcagtg gtgaaatctt cgagcttaac tcggaaactg ccattgatac tatctgtctt
      121 gaatcatctg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacgcccat tgcgaaggca gcttactacg gatgtattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014134             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14296 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014134
VERSION     KHUT01014134.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14296"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          105 g           39 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggccttg
       61 tcagtcaggc gtgaaagccc cgggctcaac ccgggaggtg cgcttgggac ggcgaggctg
      121 gagttcggga gaggagcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgctctgga ccgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014135             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14297 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014135
VERSION     KHUT01014135.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14297"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           99 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcgt
       61 caagtccgtg gtgaaagcct ccggcttaac cggagaagag ctgcggatac tggcgacctc
      121 gagtggtgta ggggtctgtg gaactgcggg tggagcggtg aaatgcgtag agatccgcag
      181 gaacgccggt ggcgaaagcg acggactgga caccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014136             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14298 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014136
VERSION     KHUT01014136.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14298"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           85 g           56 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaat
       61 taagtcagat gtgaaatccc taggcttaac ctaggaattg cgtttgagac tgaatagcta
      121 gagtacagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg acttcctgga ctgttactga cactgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014137             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14299 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014137
VERSION     KHUT01014137.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14299"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgtgcgca ggcggcaaag
       61 caagtcgagt gtgaaagccc cgggcttaac ttgggaattg cgctcgaaac tactttgctg
      121 gagtgtggca gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gccttctggg ccaacactga cgctcatgca cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014138             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14300 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014138
VERSION     KHUT01014138.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14300"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggagcgca ggcggatcag
       61 taagtcagtg gtgaaatacc cgtgcttaac atgggaactg ccattgatac tattggtctt
      121 gaatatcgtt gaggtttgcg gaatgggtca tgtagcggtg aaatgcatag atatgacccg
      181 gaacacctat tgcgaaggca gcaggctaaa cgaagattga cgctgaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014139             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14301 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014139
VERSION     KHUT01014139.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14301"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgtgta ggcggccgtg
       61 caagtcaagt gtgaaaagcc tcggcttaac cgaggcattg cacatgaaac tgcatggctt
      121 gagtgcagga gagggaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttcctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014140             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14302 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014140
VERSION     KHUT01014140.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14302"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta gacggttttg
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg cgttcgagac tgcatggcta
      121 gagtacggta gagggaagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg gcttcctgga ccagtactga cgttcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014141             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14303 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014141
VERSION     KHUT01014141.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14303"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           87 g           57 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccgtctta
       61 taagcgtgtt gtgaaatgtc ggggctcaac ctgggcattg cagcgcgaac tgtgagactt
      121 gagtgcgcag gaagtaggcg gaattcgtcg tgtagcggtg aaatgcttag atatgacgaa
      181 gaactccgat tgcgaaggca gcctgctgta gcgcaactga cgctgaagct cgaaagcgtg
      241 ggtatcgaac agg
//
LOCUS       KHUT01014142             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14304 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014142
VERSION     KHUT01014142.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14304"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           57 c           84 g           44 t
ORIGIN      
        1 gacgaaccat ccaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggcacgg
       61 taagttgggt gtgaaatacc tcggcccaac cgaggaattg cgcccaatac tgccgcgctc
      121 gaggaagaca taggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccagt agcgaaagcg gctcactggg tcttatctga cgctgaggca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014143             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14305 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014143
VERSION     KHUT01014143.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14305"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           90 g           44 t
ORIGIN      
        1 tacagaaagt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggctact
       61 taagtcggat gtgaaagccc ttggctcaac tgaggaagtg cacccgaaac tgagtagctg
      121 aagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014144             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14306 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014144
VERSION     KHUT01014144.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14306"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           91 g           51 t
ORIGIN      
        1 tacgggaggt gcgagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggtctt
       61 taagtcgcat gtgaaagccc tgggcttaac ccgggaattg cacgcgaaac tggagatctt
      121 gaatgcccga gaggttgatg gaattctagg tgtaggagtg aaatccgtag atatctagag
      181 gaacaccaga ggcgaaggcg gtcaactggt ggtgcattga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014145             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14307 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014145
VERSION     KHUT01014145.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14307"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatca ctgggcgtaa agggtgcgta gacggcatgt
       61 taagttcgtt gttaaaggct ccagcctaac tggagtatcg cgatgaaaac tggcatactg
      121 gagggtgaga gagagaagtg gaattcctgg agtagcggta aaatgcgtgg atctcaggag
      181 gaacaccaat ggcgaaggca gcttcttggc tcattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014146             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14308 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014146
VERSION     KHUT01014146.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14308"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           52 c           90 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggctctc
       61 ccagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgatac tagagagctg
      121 gagtctcgga gaggatagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgagtactga cactgagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014147             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14309 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014147
VERSION     KHUT01014147.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14309"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           87 g           49 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggca ggcggttcgt
       61 aaagtctgtt gtgaaagctc ccggcttaac tgggagaggc caatggatac tcacggactt
      121 gaatcgggga ggggtcactg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccaat ggcgaaggca ggtgactaga cccgtattga cgctcatcca cgaaagctgg
      241 gggatcaaac agg
//
LOCUS       KHUT01014148             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14310 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014148
VERSION     KHUT01014148.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14310"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           93 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttgtt
       61 taagcgggat gtgaaatccc cgggcttaac ctgggaacgg catcctgaac tgaacagctg
      121 gagtactgta gaggatagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actgtctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014149             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14311 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014149
VERSION     KHUT01014149.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14311"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           58 c           98 g           43 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggattta ttgggcgtaa agcgcacgca ggcggccagg
       61 caagtcggat gtgaaaaccc gaggctcaac ctcgggcctg cattcgatac tgcttggctc
      121 gaggcaggta ggggagatcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccggt ggcgaaggcg gatctctggg cctgtcctga cgctcaggtg cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01014150             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14312 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014150
VERSION     KHUT01014150.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14312"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           67 c          101 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 gaggtccggg gtgaaatccc cccgctcaac gggggaacgg ccccggatac cggcggcctc
      121 gaggtgggta ggggcggatg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg atccgctgga ccccccctga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014151             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14313 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014151
VERSION     KHUT01014151.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14313"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           92 g           53 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatga ctgggcgtaa agggtgcgta ggcggttttt
       61 taagtgaggc gtgaaagccc tgggcttaac ccaggaattg cgtttcagac tgaaaggctt
      121 gagtacgaga gaggtaagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gcttactggc tcgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014152             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14314 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014152
VERSION     KHUT01014152.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14314"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           84 g           52 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggaatca ttgggtgtaa agggcaagta ggcggcctat
       61 taagtgaggg gtgaaatcct gcgactcaat cgcagaattg cctttcaaac tgttaggctt
      121 gaggtaggaa gagggaagcg gaattcgcgg tgtaagggtg aaatctgtag atatcgcgaa
      181 gaacaccagc ggcgaaggcg gcttcctggt ccttacctga cgctgaattg cgaaagctag
      241 gggagcaaac aga
//
LOCUS       KHUT01014153             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14315 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014153
VERSION     KHUT01014153.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14315"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           94 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcgtg
       61 taagtcgaat gtgaaagccc ctggctcaac tggggagagt cattcgatac tgtacgactc
      121 gaaggcagga gagggaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actttctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014154             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14316 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014154
VERSION     KHUT01014154.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14316"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           60 c          102 g           34 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcac
       61 aaggtccggg gtgaaagctt tcggcccaac cggaaaaggg cctcggatac cggtgacctg
      121 gaggggggta ggggtgcgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gcgcactgga ccccaactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014155             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14317 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014155
VERSION     KHUT01014155.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14317"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agcgcgcgca ggtgggtgtg
       61 caagtcggaa gtgaaatctc ggagctcaac tccgaggctg ctcccgaaac tgctcgccta
      121 gggactggta gaggctggta gaattcccgg tgtagcggtg gaatgcgtag agatcgggaa
      181 gaatacccgt ggcgaaggcg gccagctggg ccagatccga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014156             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14318 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014156
VERSION     KHUT01014156.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14318"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggatttg
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgcttatctt
      121 gggccatcta ggggctaccg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca ggtagctggg gatgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014157             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14319 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014157
VERSION     KHUT01014157.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14319"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           85 g           52 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgca ggcggcatgt
       61 taagtcagtt gttaaatact tcagcctaac tgaagatcgg cggcagatac tggcatgcta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctaaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014158             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14320 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014158
VERSION     KHUT01014158.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14320"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           59 c          107 g           41 t
ORIGIN      
        1 tacgtagggt gcaagcgttg cccggattta ttgggcgtaa agggcgcgta ggcggtgccg
       61 cgcgtcgcgc gtgaaatctc ccggcttacc cgggaggggt cgtgcgatac ggcggcactc
      121 gagcgcggga gagggaagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccgtggctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014159             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14321 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014159
VERSION     KHUT01014159.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14321"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          103 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggtcctt
       61 taagtccgcg gtgaaagccc gaggctcaac ctcggagggt ccgcggagac tggaggactg
      121 gagtatcgga gaggagtgcg gaattcctgg tgtagcggtg gaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca gcactctggc cggtaactga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014160             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14322 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014160
VERSION     KHUT01014160.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14322"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           99 g           42 t
ORIGIN      
        1 tacgaggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgtgg
       61 caagtctcct gtgaaaactc cgggctcaac tcggagcctg caggagggac tgccatgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014161             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14323 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014161
VERSION     KHUT01014161.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14323"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           64 c          104 g           33 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgcg
       61 tgagtcgggg gtgaaatcgc ccggcccaac cgggcaaggg cccccgatac cgcgcgcctg
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgcgctgga cctccgctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014162             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14324 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014162
VERSION     KHUT01014162.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14324"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           90 g           51 t
ORIGIN      
        1 tacgaattgt gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctatg
       61 aaggtcaggt gtgaaatccc acggctcaac cgtggaactg cgcttgaaac cgcgtggctt
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tcttttctga cgctgaggca cgaaagccag
      241 gggagtgaac ggg
//
LOCUS       KHUT01014163             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14325 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014163
VERSION     KHUT01014163.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14325"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           85 g           50 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggcggtttga
       61 taagtccgtt gtgaaagctc ccggcttaac tgggagaggt caacagatac tgtcgaactt
      121 gagtatggga gagggtactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaagca ggtacctgga ccattactga cgctcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014164             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14327 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014164
VERSION     KHUT01014164.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14327"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           93 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaagta ggtggtctca
       61 tttgtcaggg gtgaaagcct tgggcttaac tcaagaagtg cccctgaaac ggtgagactt
      121 gagtcctgga gaggcgagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gctcgctgga caggcactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014165             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14328 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014165
VERSION     KHUT01014165.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14328"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           38 c           83 g           57 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtaata
       61 caagtctgtt attaaagacc ggagcttaac tccgggaatg taatggaaac tgtattgcta
      121 gaggatagta gaggaagtcg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgaaagcg gacttctggg ctattactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014166             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14329 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014166
VERSION     KHUT01014166.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14329"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggcctgc
       61 taagtctggt gtgaaagccc ggagcttaac tccggaagtg cactggatac tggcaggcta
      121 gaggacgaga gagggaagcg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gcttcctggc tcgtttctga cgctcagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014167             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14330 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014167
VERSION     KHUT01014167.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14330"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           90 g           50 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcggag
       61 caagtctgat gtgaaatcct ggggctcaac ctcagaactg cattggatac tgcctcgcta
      121 gagtactgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaaggccagt ggcgaaggcg agtttctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014168             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14331 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014168
VERSION     KHUT01014168.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14331"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           91 g           45 t
ORIGIN      
        1 tacgtatggt gcgaacgttg ttcggaatca ctgggcataa agagcacgta ggcggtcgag
       61 caagtccggt gtgaaatccc ccggcttaac cggggaactg caccggatac tattcgacta
      121 gagtgcagga ggggagagtg gaattccagg tggagcggtg aaatgcgtag atatctggag
      181 gaacgccggc ggcgaaagcg actctctggt ctgcaactga cgctgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014169             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14332 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014169
VERSION     KHUT01014169.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14332"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           92 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgtgta ggcggtttgt
       61 taagtcatgt gtgaaagccc tcggctcaac cgaggaaaga cgcatgaaac tgacaggcta
      121 gagtaccaaa gagggtggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccgt ggcgaaggcg gccacctggt tggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014170             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14333 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014170
VERSION     KHUT01014170.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14333"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           84 g           56 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggtggactgt
       61 caagtcagtt gtgaaatccg cgggctcaac ctgcggcctg cagttgaaac tggcggtctt
      121 gagtccgttc agggatgctg gaattcatgg tgtagcggtg aaatgctcag atatcatgaa
      181 gaactccaat cgcgaaggca ggcatccggg ccggaactga cactgaggct cgaaagtgcg
      241 ggtatcaaac agg
//
LOCUS       KHUT01014171             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14334 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014171
VERSION     KHUT01014171.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14334"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           46 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttatc
       61 taagtctggt gtgaaagccc ggggctcaac cccggaagtg cactggatac tggatagcta
      121 gagggcgaga gaggagatcg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatctgt ggcgaaggcg gatctctggc tcgtttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014172             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14335 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014172
VERSION     KHUT01014172.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14335"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           99 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggcgtaa agggtgtcta ggcggcaagg
       61 caggtcttgc gtgaaagctc ccggctcaac tgggagagcg cgcaggaaac ctcctggcta
      121 gagactcgga ggggggtgtg gaattcccgg tggagtggtg aaatgcgttg agatcgggag
      181 gaacaccagt agcgaaggcg gcaccctggc cgagttctga cgctgaagca cgacagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014173             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14336 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014173
VERSION     KHUT01014173.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14336"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           95 g           48 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggcctgt
       61 gcaagtcagt tgtgaaatct ccgagcttaa ctcggaacgg tcaactgata ctgctcggct
      121 agagtgcgga aggggcaact ggaattcttg gtgtagcggt gaaatgcgta gatatcaaga
      181 ggaacacctg cggcgaaggc gggttgctgg gccgacactg acgctgatgc gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014174             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14337 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014174
VERSION     KHUT01014174.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14337"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           50 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtttga
       61 caagtcaggt gtgaaagccc cgagctcaac tcgggaattg cgcttgagac tgtctttcta
      121 agagttcggg agaggtaagt ggaattccca gtgtagcagt gaaatgcgta gatattggga
      181 agaacacccg tggcgaaggc ggcttactgg accgacactg acgctgaggc gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014175             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14338 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014175
VERSION     KHUT01014175.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14338"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           87 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgagta ggcggctttt
       61 taagtctgat gtgaaagccc ggggcttaac cccggaagtg cattggatac tgaaaagcta
      121 gagggtgtaa gaggagagtg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatcagt ggcgaaggcg gctctctggt acatttctga cgctcagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014176             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14339 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014176
VERSION     KHUT01014176.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14339"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggttttg
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cacacgatac tgcgaagctt
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014177             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14340 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014177
VERSION     KHUT01014177.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14340"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggtgggttgt
       61 taagtcggat gtgaaatccc tcagcttaac tgaggaagtg cattcgaaac tggctgcctt
      121 gagtacggga gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014178             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14341 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014178
VERSION     KHUT01014178.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14341"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           59 c           98 g           37 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtgcctt gtgaaatccc ccggctcaac cggggaacgg ctgggcagac tggcggtctt
      121 gagccaccta agggcggacg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccgac ggcgaaagca gtccgctggg ggtgagctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014179             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14342 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014179
VERSION     KHUT01014179.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14342"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           88 g           55 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggtttat
       61 caagtcggaa gtgaaagccc agggcttaac cttggaattg ctttcgaaac tgatttacta
      121 gagattggta ggggatagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctatctgga ccaaatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014180             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14343 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014180
VERSION     KHUT01014180.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14343"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c          105 g           40 t
ORIGIN      
        1 tacggggggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggtatcg
       61 taagtctgtg gtgaaagccc ggggctcaac cccgggtctg ccgcggagac tgcgaaactt
      121 gagtgctgta gaggggggta gaatgccggg tgtagcggtg gaatgcgtag agatccggca
      181 gaagaccggt ggcgaaggcg gccccctggg cagtaactga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014181             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14345 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014181
VERSION     KHUT01014181.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14345"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           83 g           60 t
ORIGIN      
        1 tacggaggat tcaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggtcgga
       61 taagttagag gtgaaatccc ggggctcaac ttcggaattg cctctgatac tgttcggcta
      121 gagagtagtt gcggtaggcg gaatgtatgg tgtagcggtg aaatgcttag agatcataca
      181 gaacaccgat tgcgaaggca gcttaccaag ctacttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014182             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14346 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014182
VERSION     KHUT01014182.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14346"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           81 g           54 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaatg
       61 taagtcagac gtgaaattcc ggggctcaac ctcggagcgg catttgaaac tacatttcta
      121 gagggtagaa ggagaaaaca gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggcgaaggcg gttttctatt ttattcctga cgctgaagcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014183             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14347 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014183
VERSION     KHUT01014183.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14347"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           84 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgggca ggcggacatt
       61 taagtaagaa gtgaaaggtt tgggctcaac ccggacactg cttcttatac tgggtgtctt
      121 gagtgccgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg agatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cggaaactga cgctcatcca cgaaagctaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01014184             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14348 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014184
VERSION     KHUT01014184.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14348"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           63 c           98 g           35 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaataa ctgggcttaa agggcgcgta ggcgggccgc
       61 caagttcggg gtgaaatcct ccggctcaac cggagaacag ccccgaatac tggaggcctc
      121 gaggggggta ggggcacgcg gaactgtggg tggagcggtg aaatgcgttg atatccacag
      181 gaactccggt ggcgaaggcg gcgtgctgga cccctcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014185             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14349 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014185
VERSION     KHUT01014185.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14349"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           92 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggccgtt
       61 taagtccggg gtgaaagccc gttgctcaac aacggaactg ccccggaaac tggatggctt
      121 gagtacagac gagggcggcg gaatggacgg tgtagcggtg aaatgcatag ataccgtcca
      181 gaaccccgat tgcgaaggca gctgcctaga ctgtgactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014186             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14350 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014186
VERSION     KHUT01014186.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14350"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           59 c           97 g           40 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcgggcccc
       61 tcagtgcctt gtgaaatccc accgctcaac ggtggaacgg ctgggcatac tgggggcctt
      121 gagagaccta ggggtcagcg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccggc ggcgaaagcg gctgactgga ggtcttctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014187             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14351 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014187
VERSION     KHUT01014187.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14351"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           84 g           57 t
ORIGIN      
        1 tacgtagggt gcaagcgtta ctcggattta ttgggcgtaa agcgcatgta ggcggctttg
       61 taagtttgat gtgaaatccc tggactcaat tcaggaactg cattgaatac tgcaaggcta
      121 gagtacggga gaggtaggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg acttactgga ccgtaactga cgctgagatg cgacagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014188             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14352 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014188
VERSION     KHUT01014188.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14352"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           48 c           82 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggccggagag
       61 aaagtcagat gtgaaatcct ggggctcaac ctcagaacag catttgaaac tcctcttcta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014189             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14353 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014189
VERSION     KHUT01014189.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14353"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           96 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggatata
       61 tgagtgtgat gtgaaagccc tgggcttaac ctaggaagtg cattgcaaac gatgtatctg
      121 gagtacaaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttgatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014190             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14354 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014190
VERSION     KHUT01014190.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14354"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           88 g           55 t
ORIGIN      
        1 tacgagtgct tcaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggcggcgatg
       61 ttagtctctc gttaaattct tcggcttaac cgggggctcg cgggagaaac ggcattgctt
      121 gaggatggaa ggggtctgcg gaactcatgg tgtagcggtg aaatgcgttg atatcatggg
      181 gaacaccgaa agcgaaggca gcagactggt ccactcctga cgctgaaaca cgaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01014191             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14355 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014191
VERSION     KHUT01014191.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14355"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           48 c          105 g           46 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agggcgcgta ggcgggaact
       61 taagtccgct gtgaaagccc ggggcttaac cccggagggt cggtggagac tgggtttctt
      121 gaaagggcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctgga gcctgtttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014192             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14356 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014192
VERSION     KHUT01014192.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14356"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           92 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcga
       61 taagtctgct gtgaaagtcc agggctcaac cctgggatgc cggtggatac tgtcgagctc
      121 gagtccggaa gaggtgtgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat agcgaaggca gcacactggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014193             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14357 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014193
VERSION     KHUT01014193.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14357"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           58 c           95 g           38 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatggg
       61 aacgtcggac gttgaaagcc cccggctcaa ccggggaagg ggcgccgaaa cgacctgtct
      121 cgaggaacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgttatctg acgctgagac gcgaaagcta
      241 ggggagcaaa cggg
//
LOCUS       KHUT01014194             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14358 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014194
VERSION     KHUT01014194.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14358"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           88 g           51 t
ORIGIN      
        1 tacgtaggtg gcgagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggttcgg
       61 taagtctttt gtgaaagctc ccggcttaac tgggagaggc caagggaaac tgctgggcta
      121 gaatgtggta gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgtaagca ggcttctgga ccatcattga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014195             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14359 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014195
VERSION     KHUT01014195.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14359"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           82 g           51 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggctatg
       61 ttagtctcat gttaaaactc ccggcttaac cgggaaaccg catgggaaac ggcatatcta
      121 gaggatgcga ggggtctgta gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaataccaaa agcgaaggca gcagactggc gcactcctga cgctgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014196             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14360 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014196
VERSION     KHUT01014196.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14360"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           58 c          106 g           39 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggaatta ctgggcgtaa agcgtgcgca ggcggctcct
       61 taggcatgac gtgaaagccc cgggctcaac ccgggagggt cgtcgtgaac cggggggctt
      121 gaggccggta gaggtgggtg gaactcctgg tggagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgaaggcg gcccactggg ccggtgctga cgctgaggca cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014197             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14361 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014197
VERSION     KHUT01014197.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14361"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           83 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggttgtctat
       61 caagctagtg gtgaaagccc ggagctcaac tccggaactg ccattagaac tgataggcta
      121 gagagtgtta gaggtgagtg gaatttctag tgtaggagtg aaatccgtag atattagaag
      181 gaacatcaat ggcgaaggca gctcactggg tcacatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014198             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14362 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014198
VERSION     KHUT01014198.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14362"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           90 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttg
       61 taagtgagat gtgaaagacc tgggcttaac ctaggaagtg catttcagac tgcaaagcta
      121 gagtgcgaga gaggaaagtg gaatgacgag tgtagaggtg aaattcgtag atattcgttg
      181 gaacaccagt ggcgaaggcg actttctggc tcgcaactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014199             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14363 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014199
VERSION     KHUT01014199.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14363"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           94 g           46 t
ORIGIN      
        1 gacgtagggg gccagcgttg ctcggaatta ctgggcgtaa agggttcgta ggcggtgcgg
       61 caagttggga gtgaaatctc tgggcttaac ccagaggctg cttccaaaac tgccgcgctt
      121 gagtacggga gaggcgagtg gaattgcagg tgtagcggtg aaatgcgtag atatctgcag
      181 gaacacccgt ggcgaaagcg gctcgctgga ccgttactga cgctgaggaa cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014200             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14364 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014200
VERSION     KHUT01014200.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14364"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           53 t
ORIGIN      
        1 tacagggggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggatcgt
       61 taagtctggt gtgaaatccc tcggctcaac tgaggacgtg cattggatac tggcgacctt
      121 gagtcctgga gagggtagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagtgactga cgctgagacg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014201             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14365 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014201
VERSION     KHUT01014201.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14365"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           49 c          102 g           49 t
ORIGIN      
        1 tacggagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcgggtcag
       61 caagtctgct gtgaaagcct gaggcttaac ctcagcgggt cggtggatac tgctggtctt
      121 gaaggtgcta gggggagatg gaattaccgg tgtagcggtg aaatgcgtag atatcggtag
      181 gaacaccagt ggcgaaggcg gtctcctggg gcattcttga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014202             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14366 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014202
VERSION     KHUT01014202.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14366"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           41 c           80 g           62 t
ORIGIN      
        1 tacggaggat tctagcgttg tccggattta ttgggtttaa agggtgcgta ggcggatgac
       61 taagtcaggg gtgaaagccg gcagcttaac tgtcgaaatg cctatgaaac tggtcatctt
      121 gaatatagtt gaggtgggcg gaatgtgaca tgtagcggtg aaatgcttag atatgttaca
      181 gaacaccgat tgcgaaggca gctcactaag ctattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014203             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14367 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014203
VERSION     KHUT01014203.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14367"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           86 g           49 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgtc gtggaagctc ccggcttaac tgggagaggt cgacagaaac tatcaagctt
      121 gagtgtggga gagggtattg gaattcccgg tgtagcggtg aaatgcgcag agttcgggag
      181 gaacaccaat ggcgaaagca gatacctgga ccactactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014204             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14368 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014204
VERSION     KHUT01014204.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14368"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           96 g           48 t
ORIGIN      
        1 tacgggagag gcaagcgttg ctcggaatca ttgggcttaa agcgcgtgta ggcggctcgt
       61 caagtgccat gtgaaagtcc tcggctcaac cggggaaatg cacggtatac tgtcgggctt
      121 gaggtgggta ggggtgcctg gaactctagg tggagcggtg aaatgcgttg atatctagag
      181 gaacgcccgt ggcgaaagcg gggcactggg ccttacctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014205             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14369 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014205
VERSION     KHUT01014205.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14369"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcggga
       61 taagtctgat gtgaaatctt cgagcttaac tcggaaatgg cattggaaac tattttgcta
      121 gaggtttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014206             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14370 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014206
VERSION     KHUT01014206.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14370"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           93 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtcttc
       61 taagtctggt gtgaaagccc ggggcttaac tccggaagtg cactggatac tggaagacta
      121 gagggcgaga gggggaagcg gaattcttgg tgtaggggtg aaatccgtag atatcaagag
      181 gaacatcggt ggcgaaggcg gcttcctggc tcgtttctga cgctcagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014207             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14371 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014207
VERSION     KHUT01014207.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14371"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           97 g           49 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggtctgg
       61 taagtcagtt gtgaaatccc tcggctcaac cgaggaattg cttctgatac tgccgggctg
      121 gagtcccgga gagggcagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctgcctgga cgggtactga cgctgatgcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014208             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14372 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014208
VERSION     KHUT01014208.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14372"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           92 g           51 t
ORIGIN      
        1 tacgaagggt cctagcgtta atcggagtta ctgggcttaa agggtgcgta ggcggatcgg
       61 taagtgcttt gtgaaatccc acggctcaac cgtggaattg cttggcatac tgtcggtctt
      121 gaggtcggaa gaggtcgccg gaactctagg tgtagcggtg aaatgcgtag atatctagag
      181 gaacgcccaa ggcgaaggca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014209             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14373 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014209
VERSION     KHUT01014209.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14373"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           58 c          102 g           37 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattta ctgggcgtac agcgcgcgca ggcgggtctc
       61 gacgtgccgt gtgacaggca tcggctcaac cgatgcaggc cagggtacac gagagacctg
      121 gaggcagaga gagggatgtg gaatacctgg tggagcggtg aaatgcgtag agatcaggtg
      181 gaacaccagc ggcgaaggcg gcatcctggc tctgttctga cgctgaggcg cgagagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01014210             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14374 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014210
VERSION     KHUT01014210.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14374"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggatact
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaattg catctgatac tgtgtatcta
      121 gagtatggta gaggatggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctatctggg ccaatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014211             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14375 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014211
VERSION     KHUT01014211.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14375"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           55 c          109 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg cccggaatca ttgggcgtaa agggcgcgca ggcggcctga
       61 ttagtcgtgg gtgaaatcgt tcggctcaac cgagcggggt cctgcgaaac ggtcgggctg
      121 gagcaaggca gggggtcgtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg cctgtgctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014212             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14376 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014212
VERSION     KHUT01014212.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14376"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           84 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgta ggtggcttta
       61 taagttagtt gtgaaatccc cgggcttaac ctgggaactg cagctaatac tgtatcgcta
      121 gagtagagta gaggaaagta gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaataccagt ggcgaaggcg gctttctgga ctcatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014213             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14377 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014213
VERSION     KHUT01014213.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14377"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           91 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttgt
       61 tatgtcgggt gtgaaagccc tcagcttaac tgaggaagtg cgcccgaaac tggcaagctt
      121 gagtgtcgga gaggatagcg gaacacccgg tgtagaggtg aaattcgtag atatcgggta
      181 gaacaccagt ggcgaaggcg gctatctgga cgacaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014214             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14378 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014214
VERSION     KHUT01014214.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14378"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           93 g           45 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggtcact
       61 taagtcggat gtgaaaaccc tcggctcaac tggggaagtg cacccgaaac tgagtggcta
      121 gagtacgaaa gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014215             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14379 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014215
VERSION     KHUT01014215.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14379"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           91 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta gacggctagg
       61 caagtctggt gtgaaaaccc ggggctcaac tccgggatcg cattggaaac tgtctagcta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014216             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14380 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014216
VERSION     KHUT01014216.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14380"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcgggaact
       61 taagtctggt tttaaagacc ggggctcaac cccgggagtg gactggatac tgagtttctt
      121 gatctctgga gaggtaaccg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccaat ggcgaaggca ggttactgga cagaagatga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014217             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14381 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014217
VERSION     KHUT01014217.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14381"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          103 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggaacgt
       61 tatgtctggc ctgaaagccc ccggctcaac cggggagggt ggctggaaac tggcgttctt
      121 gagggcgtta gaggagggtg gaattcctgg agtagcggtg aaatgcgtag atatcaggag
      181 gaacaccgat ggcgaaggca gccctctggg gcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014218             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14382 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014218
VERSION     KHUT01014218.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14382"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           90 g           48 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggccggtatg
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctttgatac tgcagaccta
      121 gagtccggaa gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gcccactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014219             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14383 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014219
VERSION     KHUT01014219.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14383"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttaga
       61 taagttaggt gtgaaatgtc tgggcttaac ctggagactg cacttaagac tgtgtgacta
      121 gagtagagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctcatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014220             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14384 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014220
VERSION     KHUT01014220.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14384"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           86 g           49 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agcgtgcgca ggcggtttcg
       61 caagtcagaa gtgaaagccc agggctcaac cctggaattg cttttgaaac tgcgaagcta
      121 gagtatcgga gaggttggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgtaagcg gccaactaga cgattactga cgctcaggca cgaaagtatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014221             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14385 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014221
VERSION     KHUT01014221.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14385"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           86 g           55 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggcttta
       61 taagtcagga gtgaaagccc taggctcaac ctaggaagtg cttttgatac tgtagagctt
      121 gagtgtagga gaggatcgtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcgatctggc ctaacactga cactcagacc cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01014222             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14386 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014222
VERSION     KHUT01014222.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14386"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           81 g           59 t
ORIGIN      
        1 tacggggggt cctagcgttg ttcggaatca ctgggcgtaa agcgtttgta ggcggctttg
       61 taagttgaat gtgaaatcct ggggcctaac cccagaagtg cattcaatac tgctaagctt
      121 gagtatgaga gaggaagatg gaatagctgg tgtagtggtg aaatacgtag atatcagctg
      181 gaacatcggt ggcgaaagcg gtcttctggc tcaatactga cgctgaaaaa cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014223             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14387 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014223
VERSION     KHUT01014223.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14387"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           90 g           47 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcttgt
       61 taagtcagtc gtgaaagccc acggctcaac cgtggaagtg caactgaaac tggcaagctt
      121 gagttctgga gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggc ggcgaaggcg gccacctgga aagcgactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014224             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14388 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014224
VERSION     KHUT01014224.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14388"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcgtaa
       61 caagtcagag gtgaaagccc acagctcaac tgtggaactg cctttgaaac tgttatgctt
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014225             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14389 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014225
VERSION     KHUT01014225.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14389"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          106 g           40 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggttctg
       61 caggtggctc gtgaaagcgc ccggctcaac tgggcagggt cgggccaaac ggcagagctg
      121 gaggctggta gggggaagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctgga ccagacctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014226             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14390 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014226
VERSION     KHUT01014226.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14390"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           57 c          106 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg cccggattta ttgggcgtaa agcgcgcgca ggcgggtctg
       61 tgcgtcgtgc gtgaaatcac ccggctcaac tgggtggggt cgtgcgatac ggcggacctg
      121 gagcgaggca gagggtagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctacctgga cctcggctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014227             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14391 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014227
VERSION     KHUT01014227.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14391"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttagg
       61 aaggtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac caactagctg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaagcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014228             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14392 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014228
VERSION     KHUT01014228.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14392"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           45 a           65 c          112 g           31 t
ORIGIN      
        1 gacgtaggag acgagcgttg tccggattta ctgggcgtaa agggcgcgca ggcggtcgcg
       61 cgcgtcgggc gtgaaagccc ccggctcaac cggggcgggt cgctcgagac ggcgcgactg
      121 gagggcggca gaggctggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacacccgt ggcgaaggcg gccagctggg ccggccctga cgctgaggcg cgaaggcccg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014229             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14393 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014229
VERSION     KHUT01014229.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14393"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           95 g           51 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggattta ttgggcgtaa agcgcacgca ggcggctcga
       61 caagtctgac gtgaaatctc ccggcttaac tgggagcggt cgttggaaac tgtcgagctt
      121 gaggcgatga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcggggg
      181 gaacatcagt ggcgaaagcg gcaccctggc attagcctga cgctcatgtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014230             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14394 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014230
VERSION     KHUT01014230.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14394"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           92 g           49 t
ORIGIN      
        1 tacagagggt gctagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcagg
       61 taagtcaaat gtgaaagccc agggctcaac cttggaagtg catgtgaaac tgcctgactt
      121 gaatctcgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cgaagattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014231             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14395 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014231
VERSION     KHUT01014231.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14395"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           86 g           54 t
ORIGIN      
        1 tacgggggga gctagcgtta ttcggaatta ctgggcgtaa agagcgcgta ggcggttctg
       61 taagttggaa gtgaaagccc agggcttaac cctggaactg ctttcaaaac tgcaggacta
      121 gagattgata ggggatagcg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga tcaatactga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014232             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14396 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014232
VERSION     KHUT01014232.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14396"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c           99 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggttcgt
       61 taagtccggt gtgaaagctc ccggctcaac tgggagaggt catcggatac tggcggactt
      121 gaggaaggta gaggaaggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg cctttcctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014233             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14397 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014233
VERSION     KHUT01014233.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14397"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggcgata
       61 caagtcagag gtgaaatccc caagcttaac ttgggaactg cctttgaaac tgtattgcta
      121 gagtattgga ggggagtggg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg ccactctgga caataactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014234             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14398 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014234
VERSION     KHUT01014234.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14398"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggtttgg
       61 taagtttgat gtgaaagccc tgggcttaac ctgggaatgg cgttgaaaac tgctgaacta
      121 gagtgggata gagggcagta gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaataccggt ggcgaaggcg gctgcctggg tcctaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014235             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14399 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014235
VERSION     KHUT01014235.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14399"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           42 c           86 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaat
       61 taagttatct gtgaaatccc tgggcttaac ctgggcaggt cagataaaac tgaataactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg actacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014236             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14400 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014236
VERSION     KHUT01014236.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14400"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           54 c          109 g           42 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggcgtaa agggcgcgca ggtggtgcgc
       61 taagtccgtc gtgaaatctc ccggctcaac tgggagggtg cggcggggac tggcggactg
      121 gaggcactca gaggccggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccggctggg ggtgacctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014237             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14401 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014237
VERSION     KHUT01014237.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14401"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           94 g           44 t
ORIGIN      
        1 tacagagatg gcaagcgttg ttcggaatca ctgggcttaa agcgcgtgta ggcggagcga
       61 aaggcgtctt gtgaaatccc ccggctcaac cggggaattg ctgggcgaac cggcgctctt
      121 gaggcaggta gaggtcactg gaaccatagg tggagcggtg aaatgcgtag agatctatgg
      181 gaacgccggt ggcgaaagcg agtgactggg cctgatctga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014238             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14402 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014238
VERSION     KHUT01014238.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14402"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           84 g           57 t
ORIGIN      
        1 tacgtaggat gcaagcgttg ttcggaatta ctgggcgtaa agagtacgta ggcggtttga
       61 taagtctgct attaaagact ggagcttaac tccaggactg tagtggaaac tgtcagacta
      121 gaggacaata gaggaagtgg gaattctcag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccagt ggcgaaagcg cacttctggg ttgttactga cgctgaggta cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014239             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14403 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014239
VERSION     KHUT01014239.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14403"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           92 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgtgta ggcggccatg
       61 taggtctgac gtgaaattcc gaggctcaac ctcggcaggt cgttggaaac catatggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggaa
      181 gaacaccaat ggcgaaggca gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014240             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14404 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014240
VERSION     KHUT01014240.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14404"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           82 g           51 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggaagg
       61 aaagtcagat gtgaaatcct ggggctcaac ctcagaacag catttgaaac tgtctttcta
      121 gagggtagaa ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatt ttacacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014241             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14405 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014241
VERSION     KHUT01014241.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14405"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           50 c           71 g           51 t
ORIGIN      
        1 tacagagacc ccgagcatta tccggaatta ctgggcgtaa agagcgaaaa gccggttgcg
       61 ttagtccgat gttaaatcat agggcccaac tctatgtcag cattggaaac ggcacaactc
      121 gagtagataa gaggagagca gaacatatgg tgtaggggtg aaatccactg atatcatatg
      181 gaataccaaa agcgaaggca gctctctagt gtcttactga cggtgcatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01014242             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14406 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014242
VERSION     KHUT01014242.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14406"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           95 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggttcgg
       61 taagtctgct gtgaaagtcc agggctcaac cctgggatgc cggtggatac tgtcgagcta
      121 gagtgcggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014243             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14407 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014243
VERSION     KHUT01014243.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14407"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           45 c           92 g           56 t
ORIGIN      
        1 tacgtgaggg gcgaacgttg ttcggtgtca ctgggcttaa agggtgcgta ggtggttgtg
       61 taagtaacgt gtgaaatccc tcggcttacc cggggaattg catgttatac tgcatgactt
      121 gaggtctaaa ggggaaaagg gaactccagg tgtagcggtg aaatgcgcag atatctgggg
      181 gaaggccggc ggcgaaggcg cttttctggt tagatcctga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014244             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14408 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014244
VERSION     KHUT01014244.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14408"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           49 c           79 g           50 t
ORIGIN      
        1 tacgaatgga gcgagcgttg ttcggaatca ctgggcttaa agcgtatgca ggcggtcaag
       61 gaagtcagaa gtgaaatccc cgggcttaac ccgggaattg cttttgaaac tccatgacta
      121 gaatcccgga gaggttaaca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgcaagcg gttaactgga cgggcattga cgctcagata cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014245             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14409 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014245
VERSION     KHUT01014245.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14409"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          108 g           37 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggttaca
       61 cacatcggcg gtgaaagccc cccgctcaac gggggagggt ccgtcgagat ggtgtgactg
      121 gaggcaggga gaggcgagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gctcgctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014246             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14410 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014246
VERSION     KHUT01014246.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14410"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           88 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatca ctgggcgtaa agggcgtgta ggcgggcgga
       61 caagtcagat gtgaaatccc caggctcaac ctgggaactg catttgaaac tgtccacctt
      121 gagtgtcgga gaggaaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctttctgga cgacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014247             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14411 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014247
VERSION     KHUT01014247.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14411"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaatccc tcagctcaac tgaggaagtg cgcccgaaac tgcagagctt
      121 gagtaccgga gaggatggtg gaattcttca agtagaggtg aaatttgtag atatgaggag
      181 gaacaccggt ggcgaaggcg accatctgga cggatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014248             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14412 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014248
VERSION     KHUT01014248.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14412"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgtt ggtggttgag
       61 taagtttgat gtgaaagccc cgggcttaac ctgggaatgg cattgaatac tgctgaacta
      121 gagtcggaca gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagt ggcgaaggcg gctgcctggg tccagactga cacttaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014249             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14413 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014249
VERSION     KHUT01014249.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14413"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           55 c           90 g           41 t
ORIGIN      
        1 tacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggagctg
       61 caagtcagat gtgaaatccc cgagctcaac tcgggaactg cacctgaaac tgcagcacta
      121 gagtgtcgga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gccacctgga cgaccactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014250             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14414 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014250
VERSION     KHUT01014250.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14414"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           82 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtaatg
       61 taagttagat gtgaaatccc cgagcttaac ttgggaactg catttaagac tgcatagcta
      121 gagtagaata gaggtaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttactgga ttcatactga cgctgaggcg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014251             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14415 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014251
VERSION     KHUT01014251.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14415"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta tccggattta ctgggtgtaa agggcgtgta ggcgggactg
       61 caagtcagat gtgaaattcc agggctcaac cctggacctg catttgaaac tgtggttctt
      121 gagtgatgga gaggcaggcg gaattccctg tgtagcggtg aaatgcgtag atatagggag
      181 gaacaccagt ggcgaaggcg gcctgctgga cattaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014252             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14416 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014252
VERSION     KHUT01014252.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14416"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           84 g           53 t
ORIGIN      
        1 tacagaggtt cctagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggtgtgg
       61 taagtcaaat gtgaaatact gcagcttaac tgtagaactg cattcgaaac tgccatgcta
      121 gagggctgga gaggagatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg gatctctgga cagcaactga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014253             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14417 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014253
VERSION     KHUT01014253.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14417"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggttggg
       61 taagtcagat gtgaaatccc tgggcttaac tcaggaactg catttgatac tgcttaactt
      121 gagtacggta gagggtagtg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctacctgga tcgttactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014254             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14418 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014254
VERSION     KHUT01014254.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14418"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           84 g           55 t
ORIGIN      
        1 tacgaagggt gcaaacgtta ttcggaatga ctgggcgtaa agggcgcgta gacggtatat
       61 taagtctgtt gttaaattct ttggcctaac caagggtaag caccagatac tgatgtgctt
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014255             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14419 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014255
VERSION     KHUT01014255.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14419"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          100 g           41 t
ORIGIN      
        1 tacgtaggtg gcgaacgtta tccggaatta ctgggcgtaa agggtccgca ggcggcggag
       61 gaggtctggt gtgacagccc gtggctcaac tacgggaggc cactggaaac cgctccgctt
      121 gaatcaggcc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctagg ccctgattga cgctgaggga cgagagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014256             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14420 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014256
VERSION     KHUT01014256.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14420"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           60 c          100 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 caagtccggg gtgaaagccc ccagctcaac tggggaagcg ccttggatac ttcgggcctg
      121 gagggaggta ggggcgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014257             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14421 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014257
VERSION     KHUT01014257.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14421"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           98 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgag
       61 aaagtccggg gtgaaagcgt acggctcaac cgtaccaggg ccttggatac tgctcgtctc
      121 gagggaggta ggggcgtgcg gaacttccgg tagagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcacgctggg cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014258             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14422 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014258
VERSION     KHUT01014258.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14422"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 gacagaggcc ccgagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcgggtcga
       61 taagtgtcgt gtgaaagccc actgctcaac ggtggaactg cacggcaaac tgtcggtctt
      121 gaagaattca gaggtcgctc gaacgcatgg tggagcggtg gaatgcgtag atatcatgcg
      181 gaatgccaaa ggtgaagaca ggcgactggg gatttcttga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014259             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14423 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014259
VERSION     KHUT01014259.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14423"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggctacg
       61 aaagtcggat gtgaaagccc tgggctcaac ccaggaagtg cactcgatac tgcgtagctt
      121 gagtcccgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cggagactga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014260             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14424 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014260
VERSION     KHUT01014260.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14424"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           86 a           32 c           65 g           70 t
ORIGIN      
        1 tacaggggat gcaagtgtta tccggaatta ttgggcgtaa agcgtctgca gattgatatt
       61 taagtctttt gttaaaactt tgtgcttaac tcaaaaattg caaaagaaac tatttatctt
      121 gggtttagta gaggtaaagg gaattttcag tggagcggtg aaatgcgtag atattgaaaa
      181 gaacaccaat agcgaaggca ctttactgga tttggactaa cattaaaaga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014261             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14425 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014261
VERSION     KHUT01014261.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14425"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           60 c           95 g           37 t
ORIGIN      
        1 gacaggggcc ccaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggaccgc
       61 aaagcgtctt gtgaaatccc ccggctcaac cggggaaccg cagggcgaac tggcggtctc
      121 gaggcaagta ggggcgggca gaacagtcga tggagcggtg aaatgcgtag atatcgactg
      181 gaatgccgat ggtgaagacg gcccgctggg cttgtcctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014262             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14426 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014262
VERSION     KHUT01014262.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14426"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           87 g           52 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggttgga
       61 aaagtcagat gtgaaattcc ggggctcaac ctcggagctg catttgaaac tttctagcta
      121 gagttcagtt agggaaaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccggt ggtgaagacg gttttctggg ctgaaactga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014263             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14427 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014263
VERSION     KHUT01014263.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14427"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           83 g           56 t
ORIGIN      
        1 tacgagtgct ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggttgtg
       61 ttagtcttct gttaaattct tcggcttaac cgggggcatg caggggaaac ggcacaacta
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca attcactggc gcactcctga cgctgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014264             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14428 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014264
VERSION     KHUT01014264.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14428"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           86 g           55 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggattta ctgggcgtaa agcgcgcgta ggcggcgata
       61 caagtcaggg gtgaaatcct ggggcttaac cccagaattg cctttgaaac tgtattgcta
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgcttaggcg cgaaagcgtg
      241 ggtatcaaac agg
//
LOCUS       KHUT01014265             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14429 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014265
VERSION     KHUT01014265.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14429"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           44 c           79 g           55 t
ORIGIN      
        1 gacgtaggct gcaagcgtta tccggaatta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagttggat gttaaagctc agggcccaac cctggtcagg tgttcaaaac tggcaaacta
      121 gagacaagta gaggcaagta gaattctgca tgtaggggta atatccgtag atatgcagag
      181 gaataccaaa agcgaaggca gcttgctgga cttgttctga cgctcaagaa cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014266             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14430 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014266
VERSION     KHUT01014266.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14430"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           83 g           58 t
ORIGIN      
        1 gacagagggt gcaagcgtta atcggaatta ttgggcgtaa agcgcgcgta ggtggattaa
       61 taagttggat gtgaaatccc tgggcttaac ctgggaattg cattcaatac tgttattcta
      121 gagtacagta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg acttcctgga ctgatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014267             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14431 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014267
VERSION     KHUT01014267.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14431"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           89 g           50 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agagcgtgta ggcggcccgg
       61 aaagtctggt gtgaaaactt gaggctcaac ctcaagcttg catcggatac tgtcgggctt
      121 gaatctggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctctctggt acatgattga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014268             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14432 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014268
VERSION     KHUT01014268.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14432"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           37 c           84 g           60 t
ORIGIN      
        1 tacagagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtttat
       61 taagttagaa gtgaaatccc ggagcttaac ttcggaattg cttttaatac tgataagcta
      121 gagattggta ggggatagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacaccagt ggcgaaggcg gctatctgga ccaatactga cgctgaggca cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014269             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14433 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014269
VERSION     KHUT01014269.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14433"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggctgtttgt
       61 taagtcttct gtgaaagcta cgggctcagc ctgtaaagtg cagaggaaac tagcagactc
      121 gagtgtcgga gaggataggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg cctatctggc tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014270             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14434 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014270
VERSION     KHUT01014270.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14434"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggttact
       61 taagtcaaat gtgaaagccc cgggctcaac ctgggaactg cacgtgatac tgggtaacta
      121 gagtatggta gaggatggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gccgtctggg ccaatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014271             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14435 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014271
VERSION     KHUT01014271.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14435"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           96 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggatgtc
       61 caagtccggg gtgaaatcct ccggcttaac cggagaacag ccttggatac tggacgtctc
      121 gagggaggta ggggtcgagg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014272             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14436 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014272
VERSION     KHUT01014272.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14436"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacgaaggcc ccgagcgtta tccggaatta ttgggcgtaa agggtgcgta ggtggttttg
       61 tgcgtctctt gttaaatttc actgctcaac agtgaagccg caagggaaac ggcagaacta
      121 gaggatgcaa gaggtctgta gaactcacgg tgtaggggtg aaatccgttg atatcgtggg
      181 gaataccaaa ggcgaaggca gcagactggt gcatttctga cactgaggca cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01014273             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14437 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014273
VERSION     KHUT01014273.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14437"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           55 c           90 g           44 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggatctt
       61 taagtcgggg gtgaaatccc ggagctcaac tccggaactg ccctcgatac tgaagacctt
      121 gagtccggga gaggtgagag gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014274             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14438 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014274
VERSION     KHUT01014274.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14438"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           87 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgta ggcgggagta
       61 caagtcaggt gtgaaatcta tgggctcaac ccataaactg cacttgaaac tgtatttctt
      121 gagtgatgga gaggcaggcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcctgctgga cattaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014275             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14439 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014275
VERSION     KHUT01014275.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14439"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           92 g           49 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatca ctgggcgtaa agcgcacgta ggcggatttg
       61 taagtcaggg gtgaaatccc ggggctcaac ctcggaactg cctctgatac tgcggatctc
      121 gagtccggaa gaggtgggtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgagggcg gcccactggt ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014276             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14440 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014276
VERSION     KHUT01014276.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14440"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           96 g           43 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgtgta ggcggtaggg
       61 tgtgtctgac gtgaaagccc tcggctcaac cgaggaaatg cgtcggaaac taccttgctg
      121 gagtgcgaga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag ataccaggag
      181 gaacaccaga ggcgaaggcg gccacctggc tcgtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014277             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14441 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014277
VERSION     KHUT01014277.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14441"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           84 g           53 t
ORIGIN      
        1 tacagaggtt gcaagcgttg ttcggattta ttgggcgtaa agggtccgta ggcggtccgt
       61 aaagtctgat gtgaaatccc acagctcaac tgtggaactg cattggaaac tcacggacta
      121 gagtatgaga ggggttaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacacctgt cgcgaaggcg cttaactggc tcattactga cgctcaggga cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014278             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14442 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014278
VERSION     KHUT01014278.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14442"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           90 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgcacgca ggcggcttgg
       61 taagtcagtt gtgaaactcc tgggctcaac ttgggaactg cagctgatac tgccatgctt
      121 gagtacggaa gaggggagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gctccctggt ccgatactga cgctcatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014279             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14443 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014279
VERSION     KHUT01014279.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14443"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtcatc
       61 taagtctggt gtgaaatccc tcggctcaac tgaggaagtg cattggatac tggatgacta
      121 gaggatgaga gaggagagtg gaattcccgg tgtaggggtg aaatccgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gctctctggc tcatttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014280             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14444 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014280
VERSION     KHUT01014280.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14444"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgtgtgca ggctgttagc
       61 taagtcgagt gtgaaatccc tgggcttaac cgaggaagtg cattcgaaac tagctagcta
      121 gaagacggca gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaataccggt ggcgaaggcg gccttctgga ccgttcttga cgctgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014281             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14445 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014281
VERSION     KHUT01014281.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14445"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           50 c           83 g           49 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgca ggttccacga
       61 aaagtctgat gtaaaagcac tggagctcaa ctccgtgtac gtgtcggaaa ctctcgtggt
      121 agagtcactc agaggtatct ggaatgccgt gtgtaggggt aaaatccgta gatccacggt
      181 ggaacgccta aagcgaaagc aggatactgg gagtgtactg acactcagag acgaaagcgt
      241 ggggagcaaa gggg
//
LOCUS       KHUT01014282             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14446 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014282
VERSION     KHUT01014282.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14446"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           95 g           46 t
ORIGIN      
        1 tacagagggg gcgagcgtta ttcggaatta ttgggcgtaa agggcgcgta ggtggcttga
       61 caagtcaacg gtgaaatccc tcggctcaac cgaggaatgg cctttgaaac tgtcgagcta
      121 gaggccggga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctacctgga ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014283             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14447 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014283
VERSION     KHUT01014283.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14447"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           86 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 aaagttcgat gtgaaagctc cgagcttaac tcggaaatgg cattgaaaac ttccttgcta
      121 gagggttgga agggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014284             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14448 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014284
VERSION     KHUT01014284.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14448"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           87 g           56 t
ORIGIN      
        1 tacgaggggt gcgagcgtta ttcggattta ttgggcgtaa agggtgcgta gacggtttgt
       61 taagtctgtt gttaaattct ccgacttaat cggaggtagg cggtggaaac tggcaaacta
      121 gagtgtggaa gagagaagtg gaattcccgg agtagcggta aaatgcacag atctcgggag
      181 gaacaccaat ggcgaaggca gcttcttggt ccataactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014285             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14449 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014285
VERSION     KHUT01014285.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14449"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           88 g           49 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcacgta ggcggctttg
       61 caagtctgat gtgaaagccc tcggcttaac cgaggaagtg cattagaaac tgcagagctt
      121 gagtactaaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg tagatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014286             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14450 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014286
VERSION     KHUT01014286.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14450"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           85 g           53 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggctggg
       61 taagtcaaat gtgaaatcct gcagcttaac tgcagaattg cattcgaaac tgcttagctt
      121 gagggctgga gaggagatcg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaagcg gatctctgga cagcatctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014287             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14451 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014287
VERSION     KHUT01014287.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14451"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           88 g           48 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggtggtcata
       61 caagtcgggt gtgaaatccc ggagcctaac tccggaacgg cactcgaaac tgtttgactg
      121 gaggtctgga gaggtgactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaggcg agtcactgga cagaacctga cactgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014288             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14452 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014288
VERSION     KHUT01014288.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14452"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           95 g           43 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgcga
       61 caagtcacct gtgaaatccc cgggctcaac tcggggcctg caggcgaaac tgtcgtgctg
      121 gagtatggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014289             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14453 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014289
VERSION     KHUT01014289.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14453"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           41 c           83 g           55 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggattta ttgggcgtaa agggtgtgta ggcagtatat
       61 caagtctgtt gttaaatatc tcggcctaac cgagaagatg cggtggaaac tgatgtacta
      121 gaggatggga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccaat ggcgaaggca gcttcttggc ccaatcctga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014290             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14454 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014290
VERSION     KHUT01014290.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14454"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           55 c           99 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtctgtg gtgaaatcct tcggctcaac cggagaactg ccgtggatac tggtcgtctg
      121 gagggaggta ggggcagcgg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg cgctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014291             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14455 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014291
VERSION     KHUT01014291.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14455"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           95 g           50 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatta ctgggtgtaa agggagtgta ggcgggacgg
       61 caagttaggt gtgaaatgcg gaggctcaac ctccgagctg cacttaaaac tgctgttctt
      121 gagtgaagca gaggcaggcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gcctgctggg cttttactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014292             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14456 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014292
VERSION     KHUT01014292.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14456"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           64 c           97 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggatcat
       61 cacgtcggtc gctgaaagcc cccggctcaa ccggggaacg ggcgccgata cgagtgatct
      121 ggaggtaggt agggggagcc ggaactgccg gtggagcggt gaaatgcgtt gagatcggca
      181 ggaacgcccg tggcgaaagc gggctcctgg acctcacctg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014293             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14457 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014293
VERSION     KHUT01014293.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14457"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           87 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agagcacgta ggtggttgtt
       61 caagttggat gtgaaagccc cgggctcaac ctgggaaggt catccaaaac tggacgacta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga ctaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014294             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14458 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014294
VERSION     KHUT01014294.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14458"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           85 g           57 t
ORIGIN      
        1 tacatagggt gcaagcgtta tccggattta ttgggcgtaa agagttcgta ggcgtcttgt
       61 taagttctgt ttgaaatccc gaggcttaac ctcggaactg gatggaatac tggcaagatc
      121 gagtctgtta gggggtactg gaactgtcgg tgtagcagtg aaatgcgttg atatcgatag
      181 gaacaccaag ggcgaaggca ggtacctggg acacgactga cgctgaggaa cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01014295             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14459 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014295
VERSION     KHUT01014295.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14459"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggatata
       61 agagtgtggt gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac gatatatctg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cactgaagcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014296             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14460 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014296
VERSION     KHUT01014296.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14460"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           87 g           50 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggataga
       61 aaagtcagat gtgaaatctt agggctcaac cctgaaacgg cgtctgaaac tttctgtcta
      121 gaatctcgaa gagggaaggg gaatttcgca tgtaggagta aaatccgtag agatgcgaag
      181 gaacaccggt ggcgaaggcg ccttcctggg agagcattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014297             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14461 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014297
VERSION     KHUT01014297.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14461"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           83 g           58 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgtacgta ggcggcttga
       61 taagtcgatt gtgaaagccc caagctcaac ttgggaactg caatcgaaac tgtctcgctt
      121 gagtttggtc ggggatggtg gaatttctag tgtaggggtg aaatccgtag atattagaag
      181 gaatatcggt ggcgaaggcg accatctagg ccaatactga cgctaaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014298             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14462 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014298
VERSION     KHUT01014298.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14462"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           80 g           56 t
ORIGIN      
        1 tacgagtgct ccaagcgtta tccggaatta ctgggcgtaa agggtgtgta ggcggttttg
       61 ttagtcggtt gttaaagctc cccgcttaac ggggaaaatg caatcgaaac ggcaaatcta
      121 gaggatgcga gaggtgaaca gaactcatag tgtaggggtg aaatccgttg atattatggg
      181 gaataccaaa tgcgaaggca gttcactggc gcactcctga cgctgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014299             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14463 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014299
VERSION     KHUT01014299.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14463"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           84 g           59 t
ORIGIN      
        1 gacgtaggat gcaagcattg tccggattta ttgggcgtaa agagttcgta ggcggtttgt
       61 taagtctagt gttaaatatc agggctcaac cctgggactg cattggatac tggcagactc
      121 gagtgtggta gaggtaagtg gaatttctgg tgtagcggtg aaatgcgtag atatcagaaa
      181 gaacaccagt ggcgtaggcg acttactggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01014300             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14464 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014300
VERSION     KHUT01014300.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14464"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           52 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcaat
       61 taagtctggg gtgaaaggtt caacgctcaa cgtgaacagt gccttggata ctggttgact
      121 tgaatctggg agagggtagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccca tggcgaaggc agctacctgg cctaagattg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cggg
//
LOCUS       KHUT01014301             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14465 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014301
VERSION     KHUT01014301.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14465"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggatagg
       61 caagtgagat gtgaaatacc cgggcttaac ctaggaactg catcttaaac tgcttatctg
      121 gagtattgta gaggacagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actgtctgga caaatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014302             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14466 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014302
VERSION     KHUT01014302.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14466"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           42 c           81 g           57 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ctgggcgtaa agggtgcgta ggtggtttaa
       61 taagttactg gtggcaaatc ggggctcaac cccgtggaag tcagtaatac tattgaacta
      121 gagggctgga gaggttaacg gaattctgtg tgtagcggta aaatgctcaa atatacagag
      181 gaacaccaat tggcgaaggc agttaactat tcagttcctg acgttgaggc acgaaagcgt
      241 ggggagcaag acgg
//
LOCUS       KHUT01014303             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14467 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014303
VERSION     KHUT01014303.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14467"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           96 g           42 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggattta ctgggcgtaa agcgcgtgta ggcggccggc
       61 caagtcggat gtgaaagccc ggggctcaac cccggaagtg cattcgatac tggtcggctg
      121 gagtctcgga gaggcgggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggt ggcgaaggcg gcccgctgga cgaagactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014304             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14468 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014304
VERSION     KHUT01014304.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14468"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          103 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agggcacgca ggcggttgtg
       61 tcagtttgcg gggacagtgg gcggcttaac tgcccaaggg tcgcaaagac ggcacgactg
      121 gagagcgaga gagggacctg gaactccggg tggagcggtg aaatgcgtag agatccggaa
      181 gaacaccact ggcgaaggca aggtcctggc tcgtatctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014305             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14469 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014305
VERSION     KHUT01014305.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14469"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           56 c           92 g           44 t
ORIGIN      
        1 tacagaggcc tcgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcggattct
       61 taagtgtctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgggaatctt
      121 gggccactca ggggcgaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccaaa ggtgaagaca ggtcgctggg ggtgtgccga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014306             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14470 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014306
VERSION     KHUT01014306.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14470"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           90 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcga
       61 aaagtctgat gtgaaatctt ggggctcaac cccaagctgt cattggatac tgtcgagctt
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gcactctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014307             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14471 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014307
VERSION     KHUT01014307.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14471"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ttgggcgtaa agggcgcgta ggtggttagg
       61 taagttagat gtgaaagccc cgggcttaac ctgggaactg catctaatac tgccagacta
      121 gagtacagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014308             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14472 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014308
VERSION     KHUT01014308.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14472"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggcttcg
       61 taagtcaggt gtgaaacctc caggctcaac ctggagccgc catctgatac tgcgatggct
      121 tgagtccagt aggggcgcgc ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggcgcgctgg gctggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014309             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14473 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014309
VERSION     KHUT01014309.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14473"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           92 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggaaaat
       61 caagctgggt gtgaaagact taggcttaac cttgggaagt cattcaggac tggttttctg
      121 gagtatagga gaggggagtg gaattcacag tgtagcggtg aaatgcgtag atattgtgag
      181 gaacatcgat ggcgaaggca gctctctggc ctaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014310             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14474 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014310
VERSION     KHUT01014310.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14474"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           93 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgctg
       61 taagtcggat gtgaaatccc ggggctcaac cccggaactg cattcgatac tgcggtgctt
      121 gaggactgga gaggagattg gaattcacgg tgtagcagtg aaatgcttgg atatcgtgag
      181 gaaggccagt ggcgaaggcg gatctctgga cagttcctga cgctgaggca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014311             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14475 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014311
VERSION     KHUT01014311.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14475"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           88 g           55 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggttata
       61 tgtgtcaaat gtgaaatccc tgggcttaac tcaggaagtg catttgaaac tgtataacta
      121 gagtactgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcagt ggcgaaggcg accacctgga cggtaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014312             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14476 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014312
VERSION     KHUT01014312.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14476"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           97 g           40 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcgggt gtgaaaactc caggctcaac ctggagacgc cacccgatac tgctgtggct
      121 agagtccagt aggggagcgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccag cggcgaaggc ggcgctctgg gctggaactg acgctgagga gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014313             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14478 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014313
VERSION     KHUT01014313.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14478"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           89 g           51 t
ORIGIN      
        1 tacagggggt gctagcgttg ttcggaatca ctgggcgtaa agggcgcgta ggcggttttg
       61 caagtcagaa gtgaaagccc ggagcttaac tccggaattg ctcctgatac tgcaaggcta
      121 gagtgcatta gaggatggcg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccggt ggcgaaggcg gccatctggg atgcaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014314             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14479 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014314
VERSION     KHUT01014314.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14479"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           84 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggagtta ttgggcgtaa agggcgcgta ggcggtttgg
       61 taagtcttga tgtgaaattc ctgggctcaa cctaggagct gcatgggaaa ctaccaaact
      121 agaggatgga cggagaaaac ggaattccac aagtagcggt gaaatgcgta gatatgtgga
      181 agaacacctg tggcgaaggc ggttttctag tttattcctg acgctgaggc gcgaaagcaa
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014315             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14480 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014315
VERSION     KHUT01014315.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14480"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           91 g           44 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttt
       61 taagtgcctt gtgaaatccc acggctcaac cgtggaacgg ctgggcatac tgggagtctt
      121 gagctgccta ggggtcgctg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaaa ggtgaaaaca ggcgactggg ggcaagctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014316             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14481 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014316
VERSION     KHUT01014316.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14481"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           84 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggctgat
       61 taagtcagtg gtgaaaggca gcagctcaac tgttagcacg gccattgata ctggttagct
      121 tgagtacagt cgaggtaggc ggaattgaag gtgtagcggt gaaatgcata gatatcttca
      181 agaacaccta ttgcgaaggc agcttactag gctgtaactg acgctgatgc acgaaagcgt
      241 ggggatcgaa cagg
//
LOCUS       KHUT01014317             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14482 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014317
VERSION     KHUT01014317.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14482"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           47 c           88 g           57 t
ORIGIN      
        1 tacgagtgcc tcgagcgtta tccggaatta ttgggcgtaa agcgtgtgta ggtggtcatg
       61 ttagtctcct gcgaaattct tcggcttaac cggggcgtga tgggagaaac ggcatgactt
      121 gagggtgcga ggggtgtgtg gaactctagg tgtaggggtg aaatccgttg atatctaggg
      181 gaacaccaaa agcgaaggca gcacactggc gcattcctga cactgagaca ctaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014318             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14483 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014318
VERSION     KHUT01014318.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14483"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           90 g           51 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgca ggcggctctt
       61 caagtcaggg gtgaaagccc agagctcaac tctggaattg cctttgagac tgtagagctt
      121 gagtacggga gaggtgagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctcactggc ccgtttctga cgctcaggcg cgacagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014319             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14484 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014319
VERSION     KHUT01014319.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14484"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           92 g           44 t
ORIGIN      
        1 cacgggggga gcaagcgttg ttcggaatca ctgggcgtaa agcgcgtgaa ggcggttagg
       61 taagtgggac gtgaaatccc tcggctcacc cgagggactg catcccaaac tgcttagctt
      121 gagtatggga gaggatgagg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg ctcatctggc ccaatactga cgctgcagcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014320             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14485 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014320
VERSION     KHUT01014320.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14485"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           47 c           80 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggggata
       61 taagtcagac gtgaaatccc gaggctcaac ctcggaactg catttgaaac tatatctctc
      121 gagggtagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctatc ttactcctga cgctgatgcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014321             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14486 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014321
VERSION     KHUT01014321.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14486"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           77 g           64 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggctgattaa
       61 taagtcagtg gtgaaatctt cgggctcaac ccggaaattg ccgttgatac tattagtctt
      121 gaatatagtt gaggtgagca gaatgcgtgg tgtagcggtg aaatgcttag atatcacgca
      181 gaataccgat tgcgaaggca gctcgctaaa ctattattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014322             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14487 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014322
VERSION     KHUT01014322.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14487"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttagt
       61 taagtgagat gtgaaagccc tgggcttaac ctaggaaccg catcttaaac tgactagcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014323             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14488 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014323
VERSION     KHUT01014323.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14488"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           91 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ctgggcgtaa agcgagcgta ggcggccatg
       61 taagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tgtatggctc
      121 gagtactgga gagggtaacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gttacctgga cagatactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014324             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14489 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014324
VERSION     KHUT01014324.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14489"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           95 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtttcg
       61 taagtcgggt gtgaaatccc ccagcttaac tggggaagtg cgcccgaaac tgcgtgactc
      121 gagtgccgga gagggtggcg gaatacccca agtagaggtg aaattcgtag atatggggtg
      181 gaacaccggt ggcgaaggcg gccacctgga cggtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014325             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14490 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014325
VERSION     KHUT01014325.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14490"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           88 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggattta ctgggcgtaa agggcgtgta ggcgggataa
       61 taagtcagac gtgaaatacc tgagcttaac ttgggaaagt cgtatgatac tgttattcta
      121 gagtggagta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccggt ggcgaaggcg gctatctgga ctcacactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014326             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14491 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014326
VERSION     KHUT01014326.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14491"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c          102 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ttgggcgtaa agcgcgcgca ggcggcacgc
       61 taagtacgca gtgaaatctc cctgctaaac ggggagggtg ctgtgtaaac tggcgagcta
      121 gaggacggta gaggggggta gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaatacccgt ggcgaaggcg gccccctggg ccgtacctga cgctgaggcg cgaaggctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014327             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14492 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014327
VERSION     KHUT01014327.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14492"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           89 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggactta ttgggcgtaa agggcgcgta ggcggtttga
       61 taagtggggt gtgaaatact tgggctcaac ctgaggattg cattccagac tgttaagcta
      121 gaatacagca gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcaat ggcgaaggca gcttcctggg ctgatattga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014328             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14493 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014328
VERSION     KHUT01014328.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14493"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           88 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggcggctatg
       61 caagtcagag gtgaaatgcc ggagcttaac tccggaactg cctttgaaac tgcatagctt
      121 gagactcgga ggggatagcg gaattgctaa tgtagcggtg aaatgcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgagtactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014329             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14494 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014329
VERSION     KHUT01014329.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14494"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           89 g           46 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggcgggtcgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtatac tggcgatctt
      121 gagagaccta ggggctgtcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaaa ggtgaaaaca gacagctggg ggtcttctga cgctgagacg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014330             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14495 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014330
VERSION     KHUT01014330.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14495"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           86 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggatatt
       61 taagtcggat gtgaaatccc cgggctcaac ctgggaactg catacgatac tgagtatctg
      121 gagtatggta gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg actttctgga ccaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014331             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14496 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014331
VERSION     KHUT01014331.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14496"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           88 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggtaggc
       61 taagtcggat gtgaaatcct ggggctcaac cccagaactg cattcgatac tggcttgctt
      121 gaggactgga gaggagattg gaattcatgg tgtagcagtg aaatgcgtag atatcatgag
      181 gaagaccagt ggcgaaggcg gatctctgga cagttcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014332             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14497 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014332
VERSION     KHUT01014332.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14497"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           84 g           52 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtctttc gttaaaacca cgggctcaac ctgtgatcgg cgagagaaac ggcacgacta
      121 gaggatgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca gttcactggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014333             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14498 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014333
VERSION     KHUT01014333.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14498"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           86 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccaga
       61 taagtctgat gtgaaatctc cgggcttaac ccggaaatgg cattggaaac tatctggcta
      121 gaggagtgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cacttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014334             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14499 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014334
VERSION     KHUT01014334.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14499"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcatgg
       61 taggtctggg gtgaaatccc acagcttaac tgtggaagag cctccgatac cgtcatgctg
      121 gaggagggta ggggtgcgcg gaacttctgg tggagcggtg aaatgcgttg atatcagaag
      181 gaacgccggc ggcgaaagcg gcgcactgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014335             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14500 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014335
VERSION     KHUT01014335.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14500"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           82 g           57 t
ORIGIN      
        1 tacaaggggt gctagcgtta ttcggattca ttgggcgtaa agggtacgta gacggcgtgc
       61 taagttagtt gttaaatctc tcggcctaac cgagaacaag caattaatac tggtatgcta
      121 gagggtgaga gagaggagtg gaattctcgg agtagcggta aaatgcgtag atatcgagag
      181 gaacaccgat ggcgaaggca gctccttggc tcattcctga cgttgaggta cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014336             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14501 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014336
VERSION     KHUT01014336.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14501"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           88 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttagt
       61 taagtaagtt gtgaaatccc cgggcttaac ctgggaattg caagttaaac tggctagctg
      121 gagtacatga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg actacctggc atgatactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014337             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14502 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014337
VERSION     KHUT01014337.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14502"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           88 g           52 t
ORIGIN      
        1 tacgaggagc ccgagcgtta tccggattta ctgggcgtaa agggtgtgta ggtggtcttg
       61 ttagtcctgt tttaaatctc cgtgctcaac atggaggctg gatgggaaac ggcaagactt
      121 ggagggtgcc agaggcaagc ggaactcacg gtgtaggggt gaaatccgtt gatatcgtgg
      181 ggaacaccaa aggcgaaggc agcttgctgg ggcactcctg acactgaaac acgaaagcgt
      241 ggggatcaaa aagg
//
LOCUS       KHUT01014338             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14503 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014338
VERSION     KHUT01014338.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14503"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           84 g           54 t
ORIGIN      
        1 cacgtaggat ccaagcgtta tccggattta ctgggcgtaa agcgtgtgca ggtggtctgt
       61 taagttgggt gttaaatctc ttggctcaac tgggaggtgt cgctcaaaac taacagactg
      121 gaggacggta gaggaaggtg gaattccggg tgtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg accttctgga ccgctcctga cactcataca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014339             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14504 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014339
VERSION     KHUT01014339.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14504"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           49 c           88 g           47 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcaggg
       61 taagtcggat gtgaaatctc cgagctcaac tcggaaatgg cattggaaac tacccagcta
      121 gagggttgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caactcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014340             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14505 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014340
VERSION     KHUT01014340.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14505"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           93 g           45 t
ORIGIN      
        1 cacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctgag
       61 taagtcaggt gttaaatccc caggctcaac ctgggacgcc acctgatact gctctggcta
      121 gagtccggta ggggagcgtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccagc ggcgaaggcg gcgctctggg ccggaactga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014341             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14506 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014341
VERSION     KHUT01014341.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14506"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           54 c           89 g           41 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcgtgta ggcggcacgg
       61 caagtcatct gtgaaagccc tcggctcaac cgaggaagtg cagctgaaac tgccgcgcta
      121 gagtgctgaa gagggtggcg gaatgcgcag tgtagaggtg aaattcgtag atattgcgta
      181 gaacaccagt ggcgaaggcg gccacctggg aagctactga cgctaagacg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014342             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14507 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014342
VERSION     KHUT01014342.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14507"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           88 g           52 t
ORIGIN      
        1 tacgggaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggtctg
       61 caagtcggat gtgaaatccc cgggcttaac ctgggaactg cactcgatac tgcagatctt
      121 gaatacctaa gaggttgatg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacaccgga ggcgaaggcg gtcaactggt ggtgtattga cgctaatgtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014343             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14508 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014343
VERSION     KHUT01014343.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14508"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           90 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggttgtc
       61 taagtctgat gtgaaagccc agggctcaac cctggaagtg cattggatac tggataacta
      121 gagggcgaga gaggaaagtg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatctgt ggcgaaggcg gctttctggc tcgtttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014344             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14509 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014344
VERSION     KHUT01014344.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14509"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacggagact gcaagcgtta ctcggattca ctgggcgtaa agggtgcgca ggcggctttg
       61 tgtgtcagat gtgaaatgcc ggggcttaac tccggatctg cgtctgaaac tacaaggcta
      121 gagttttgga gaggtaagtg gaattcctag tgtagcagtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga caaaaactga cgctcaggca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01014345             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14510 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014345
VERSION     KHUT01014345.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14510"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           47 c           79 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtgtgca ggcggacctt
       61 taagtagaaa gtgaaaggtt ggagctcaac tccaacactg cttcctatac tgggggtctt
      121 gagtgtcgga gaggaagatg gaactacacg tgtagcggtg aaatgcgttg atatgtgtag
      181 gaacaccaat ggcgaaggca atcttctgga cgaatactga cgctgataca cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01014346             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14511 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014346
VERSION     KHUT01014346.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14511"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           45 c           79 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggtgcgta ggcgggaatg
       61 aaagtcagct gtgaaatccc ggggctcaac ctcggaactg catttgaaac tccatttctg
      121 gaggatagtc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctaga ttattcctga cgctaaggca cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014347             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14512 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014347
VERSION     KHUT01014347.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14512"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           89 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggcggtttga
       61 caagtcgggt gtgaaatccc cgggctcaac ctgggaattg cattcgaaac tgtcaagcta
      121 gagtctagga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacatcagt ggcgaaggcg gcttcctagc ctaagactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014348             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14513 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014348
VERSION     KHUT01014348.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14513"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacagaggtc ccgagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgggg
       61 caagtctgat gtgaaatcct ggagctcaac tccaggcctg cattggatac tgccttgcta
      121 gagtactgga gaggaaactg gaatttacgg tgtagcagtg aaatgcgtag agatcgtaag
      181 gaagaccagt ggcgaaggcg agtttctgga cagttactga cgctgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014349             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14514 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014349
VERSION     KHUT01014349.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14514"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           87 g           49 t
ORIGIN      
        1 gacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggctgtgcgg
       61 caagttaggt gtgaaatacc tcagcttaac tgaggaactg cgcctaaaac tgccgtgctc
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacaccggt ggcgaaagcg gctcactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014350             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14515 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014350
VERSION     KHUT01014350.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14515"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c          102 g           36 t
ORIGIN      
        1 gacggagggt gcaagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggccgcg
       61 caagtctaac gtgaaagccc agggctcagc cctggaaggg cgctagagac tgcggggcta
      121 gagtgccgga ggggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccggt ggcgaaagcg actctctgga cggcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014351             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14516 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014351
VERSION     KHUT01014351.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14516"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           43 c           75 g           57 t
ORIGIN      
        1 gacgaaggat ccaagcgtta tccggattca ttgggtttaa agggagcgta ggcgggttta
       61 taagtcagtg gtgaaagccg atcgcttaac gatcgaactg ccattgatac tgtagaccta
      121 gagtatagat gatgttggcg gaatatgaca tgtagcggtg aaatgcatag atatgtcata
      181 gaacaccgat tgcgaaggca gctgacaaaa ctataactga cgctgaggct cgaaagtgcg
      241 gggatcaaac agg
//
LOCUS       KHUT01014352             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14517 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014352
VERSION     KHUT01014352.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14517"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           83 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtac agggtgcgca ggcggtatat
       61 taagtcaact gtcaaattcc tcagcctaac tggggtccgg cggtagatac tggtatacta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014353             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14518 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014353
VERSION     KHUT01014353.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14518"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           86 g           58 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgtgtgta ggttgttttg
       61 caagtcagtt gtgaaagccc cgggctcaac ccgggaagtg cttctgatac tgcttaactt
      121 gagtactgga gaggatagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctatctggc catgtactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014354             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14519 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014354
VERSION     KHUT01014354.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14519"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggtgcgta ggtggtaagg
       61 caagtcagat gtgaaagccc ggggctcaac cccggtactg catttgaaac tgtctgactg
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014355             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14520 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014355
VERSION     KHUT01014355.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14520"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           96 g           42 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggcctgc
       61 taagtccgct ctgaaagccc agggctcaac cctgggaggc gggtggatac tggcaggctc
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014356             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14521 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014356
VERSION     KHUT01014356.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14521"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           88 g           55 t
ORIGIN      
        1 tacgagggat cctagcgttg ttcggaatta ttgggcgtaa agggtgtgta ggcggtccta
       61 caagtcagtt gtgaaagccc cgggctcaac ccgggaagtg cttctgatac tgttggactt
      121 gagtactgga gaggtgagtg gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gctcactggc catgtactga cgctgaaaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014357             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14522 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014357
VERSION     KHUT01014357.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14522"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           90 g           47 t
ORIGIN      
        1 tacggaggtc ccgagcgttg ttcggaatta ctgggcgtaa agggggcgca ggcggacagg
       61 tcagtggaaa gtggaagccc agggctcaac cctggaaagc ctttccatac tgcctgtctt
      121 gagttcgtga gaggagagcg gaattcaggg tgtagcggtg aaatgcttag atatcctgag
      181 gaacaccaat ggcgaaagca gctctctggc acgttactga cgctgaggcc cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014358             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14523 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014358
VERSION     KHUT01014358.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14523"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c           99 g           42 t
ORIGIN      
        1 gacgaaccgc ccgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgga
       61 caagtcgggg gtgaaatctt tcagcttaac tggaaaagag cttccgatac tggccgtctt
      121 gagggaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014359             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14524 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014359
VERSION     KHUT01014359.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14524"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           96 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgtc
       61 caagtcaggg gtgaaatccg gcagctcaac tgtcgaacag cttctgatac tggatgtctt
      121 gaggggggta ggggcatgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg acgtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014360             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14525 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014360
VERSION     KHUT01014360.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14525"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           98 g           46 t
ORIGIN      
        1 tacggggggg gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtctgct gtgaaaaccc gaggctcaac ctcgggcctg cagtgggtac gggcagacta
      121 gagtgcggta ggggagattg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gatctctggg ccgtaactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01014361             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14526 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014361
VERSION     KHUT01014361.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14526"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 tacgtaggat gcaagcgtta tccggaatta ttgggcgtaa agcgaacgta ggcggttctg
       61 taagtctggg gttaaagatc aaggctcaac cttgggaatg ccctggaaac tacaggactg
      121 gaggacggca gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaagcg actttctggg ccgttcctga cgctgaggtt cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014362             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14527 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014362
VERSION     KHUT01014362.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14527"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           57 c          101 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcctc
       61 ccagtccggg gtgaaagctt tcggcttaac cggaaacgtg ccttggatac tggaggtctg
      121 gaggggggta ggggcagccg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg ggctgctgga cctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014363             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14528 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014363
VERSION     KHUT01014363.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14528"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           78 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggcttg
       61 taagtcagtg gtgaaatccc ggagcttaac tccggaactg ccattgatac tataggtctt
      121 gaattttctg gaagtcagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctgactacg gaacgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014364             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14529 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014364
VERSION     KHUT01014364.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14529"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           57 c           92 g           42 t
ORIGIN      
        1 aacgtagggg gcaagcgttg cccggaatca ctgggcgtaa agcgggtgta ggcggtctct
       61 taagtcggat gtgaaatcct acggctcaac cgtggaactg catccgatac tgggagactt
      121 gaggacggta gaggtcggtg gaattccagg tgtaccggtg aaatgggcag atatctggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 gggagcaaac cgg
//
LOCUS       KHUT01014365             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14530 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014365
VERSION     KHUT01014365.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14530"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           58 c          108 g           39 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcgggcgtt
       61 taagtccggt ctgaaagccc ccggctcaac cggggaaggt ggctggaaac tgggcgtctg
      121 gagggcggca gaggcgtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccgt ggcgaaggcg gcacgctggg ccggccctga cgctgaggcg cgacagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014366             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14531 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014366
VERSION     KHUT01014366.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14531"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           89 g           57 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttcgt
       61 taagtctgat gttaaagact ggggctcaac ctcggaaatg cattggatac tggcggactg
      121 gagtacagta gaggcaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggaa
      181 gaacaccggt ggcgtaagcg acttgctggg ctgtaactga cgctgaggaa cgaaagctag
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014367             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14532 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014367
VERSION     KHUT01014367.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14532"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           99 g           46 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcttgc
       61 caagtcagcg gtgaaatctc ccggcttaac tgggaggggc ccgttgaaac tggtaggctt
      121 gagggaggca gaggaaggtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctggg ccttacctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01014368             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14533 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014368
VERSION     KHUT01014368.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14533"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c           99 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcttg
       61 atagtccgcg gtgaaagccc ccagcttaac tggggaagtg ccgtggatac tgcaagcctg
      121 gaggagggta ggggcatctg gaacgctggg tggagcggtg aaatgcgttg atatccagcg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014369             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14534 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014369
VERSION     KHUT01014369.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14534"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           98 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcc
       61 caagtccggg gtgaaatctt tcggcttaac cggaaaattg ccttggatac tgggagtctc
      121 gagggaggta ggggcagccg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccggt ggcgaaagcg ggctgctggg cctctcctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014370             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14535 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014370
VERSION     KHUT01014370.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14535"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           86 g           57 t
ORIGIN      
        1 tacgagaggt gcgagcgtta ttcggattta ttgggtgtaa agggtgcgta gacggcgcgt
       61 taagtttgtt gttaaatcct ccggctcaac cggagatatg cggcaaatac tggcgtgcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccattcctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014371             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14536 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014371
VERSION     KHUT01014371.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14536"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           59 c          103 g           37 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatga ctgggcgtaa agggcgcgta ggcggccccg
       61 tcagtcgggc gtgaaagccc cgggctcaac ccggggactg cgctcgatac ggcggggctg
      121 gaggacggaa gaggctcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgagctggt ccgttactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014372             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14537 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014372
VERSION     KHUT01014372.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14537"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           93 g           47 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcgggcatg
       61 caagtcaggt gtgaaagccc tgggctcaac ctaggaactg cacttgaaac tgcatgtctt
      121 gagtgccgga gaggatcgtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcgatctgga cggttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014373             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14538 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014373
VERSION     KHUT01014373.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14538"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           91 g           53 t
ORIGIN      
        1 tacggggggt gcaagcgttg ttcggaatta ttgggcgtaa agagcgtgta ggcggcttga
       61 taagtcagat gtgaaagccc tgggcttaac ccaggaagtg catttgaaac tgtcaggcta
      121 gagtaaggaa gggggaagtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg acttcctggt cctatactga cgctgagacg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014374             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14539 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014374
VERSION     KHUT01014374.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14539"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           56 c           92 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggagtta ctgggcgtaa agcgcatgca ggcggctcgc
       61 caagtttgag gtcacagctc ccggctcaac tgggagaaga tctcgaaaac tggcgaacta
      121 gagggctcac gagggacatg gaactccggg tgtagtggtg aaatgcgtag atatccggaa
      181 gaacaccaaa ggcgaaggca gtgtcctggg gagcacctga cgctcagatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014375             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14540 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014375
VERSION     KHUT01014375.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14540"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           98 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcattt gtgaaagctc ccggctcaac tgggagactg caagcgaaac tgccgtgctg
      121 gagtgtggga gaggtgagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gctcactgga ccacaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014376             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14541 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014376
VERSION     KHUT01014376.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14541"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           84 g           47 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggttctg
       61 taagtcgtct gtgaaatccc ggggctcaac cccggaacag catatgaaac tacaggacta
      121 gaggataggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttagccctga cgctgaggcg cgaaagcaag
      241 ggtagcaaac agg
//
LOCUS       KHUT01014377             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14542 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014377
VERSION     KHUT01014377.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14542"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           40 c           73 g           62 t
ORIGIN      
        1 tacggaggat ccaagcgttg tccggattca ttgggtttaa agggtgcgta ggcggagaat
       61 taagtcagtg gtgaaagctt acagctcaac tgtaaaaatg ccattgaaac tgattttctt
      121 gagtacgatt gaggtagatg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaggca gtctactaag tcgttactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014378             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14543 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014378
VERSION     KHUT01014378.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14543"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           86 g           51 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttga
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggt caacggatac tgtcggactt
      121 gagtatggga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca ggtaactgga ccattactga cactaagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014379             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14544 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014379
VERSION     KHUT01014379.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14544"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           96 g           48 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggagagt
       61 taagtcaggg gtgaaatgcc gaggctcaac cttggaatgg cctttgatac tggctgtctg
      121 gagatcggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggag
      181 gaacaccaga agcgaaggcg gctcactggc ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014380             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14545 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014380
VERSION     KHUT01014380.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14545"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           89 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatca ctgggcgtaa agcgctcgta ggctgtttgg
       61 tcagtcagat gtgaaagccc acggcttaac cgtggaactg catttgatac tgccagactt
      121 gagtgtcgga gagggtggcg gaattccggg tgtaggagtg aaatccgtag atatccggag
      181 gaacaccagt ggcgaaggcg gccacctgga cgacaactga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014381             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14546 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014381
VERSION     KHUT01014381.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14546"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           53 c           98 g           46 t
ORIGIN      
        1 tacgtagggc gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggttcgt
       61 cgcgtctgcc gtgaaagccc gaggctcaac ctcgggtctg cggtgggtac gggcggacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca ggtctctggg ccacaactga cgctgaggag cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014382             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14547 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014382
VERSION     KHUT01014382.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14547"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           81 g           57 t
ORIGIN      
        1 tacgaaggga gctagcgtta ctcggaatca ctgggcgtaa agcgtatgta ggcggaattt
       61 caagtcagag gtgaaatccc tgggctcaac ccaggaattg cctttgaaac tgtaattctt
      121 gagtatagta gaggatggtg gaatttccag tgtagaggtg aaattcgtag atattggaaa
      181 gaacaccggt ggcgaaggcg gccatctggg ctattactga cgctgagata cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014383             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14548 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014383
VERSION     KHUT01014383.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14548"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           90 g           50 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggcgcgg
       61 aaagtcagat gtgaaatccg ggggctcaac ctccgaattg catccgatac ttccgtgctt
      121 gagaactgaa gaggagcttg gaattctcgg tgtagcagtg aaatgcgtag agatcgagag
      181 gaacactagt ggcgaaggct gggctctggg cagtatctga cgctgaggca cgaaggtcag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014384             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14549 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014384
VERSION     KHUT01014384.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14549"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           42 c           76 g           57 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggcactt
       61 taagtcagtg gtgaaagcct agtgcttaac actagaactg ccattgaaac tgaagagctt
      121 gaatcaagga gaggtaagca gaatttatag tgtagcagtg aaatgcttag atactataag
      181 gaataccaac agcgaaggca gcttactggc cttgtattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014385             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14550 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014385
VERSION     KHUT01014385.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14550"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           90 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agtgcgcgca ggtggccgcg
       61 catgtcgagt gtgaaatccc cgagcttaac ttgggaattg cgctcgaaac tatgtggcta
      121 gagtgtggca gagggaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gcctcctggg ccaacactga cactcatgca cgaaagcgtg
      241 gggagcagac agg
//
LOCUS       KHUT01014386             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14551 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014386
VERSION     KHUT01014386.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14551"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           55 c          105 g           41 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggcggcg
       61 tatgtcggcc gtgaaagcgc acggctcaac cgtgctgggt cggcggaaac tgcgtcgctt
      121 gaggctggga ggggctggtg gaattccccg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctgga ccagtcctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014387             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14552 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014387
VERSION     KHUT01014387.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14552"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           88 g           55 t
ORIGIN      
        1 tacgtagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggctgccaat
       61 taagtcgatt gtgaaatccc tgggctcaac cgaggaagtg cattcgaaac taattggctc
      121 gaagacggta gaggaaggtg gaattcctag tgtagaggtg aaattcgtag atattaggag
      181 gaataccggt ggcgaaggcg gccttctggg ccggtcttga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014388             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14553 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014388
VERSION     KHUT01014388.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14553"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           89 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtggtttga
       61 taagtcggat gtgaaagccc tgggcttaac ctgggaacgg catacgaaac tgttgaactc
      121 gagtcgtgta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga cacagactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014389             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14555 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014389
VERSION     KHUT01014389.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14555"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           84 g           55 t
ORIGIN      
        1 tacagaggcc tcaagcgttg ttcggaatta ctgggcgtaa agcgggtgta ggttgcgtag
       61 taagtcagat gtgaaagccc tgggctcaac cctggaagtg cgtctgatac tgctacgctg
      121 gagtgcgaga gaggtaagtg gaattcctag tgtaggggtg aaatccgtag atattaggag
      181 gaataccgat ggcgaaggca acttactggc tcgtaactga cactgagatc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014390             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14556 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014390
VERSION     KHUT01014390.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14556"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           41 c           87 g           61 t
ORIGIN      
        1 tacgaagggg gcaagcgtta ttcggaatga ttgggtgtaa agggcgcgta gacggctttg
       61 taagtctgtt gtttaatttt ctggcctaac cggaagcatg cgatagatac tgctttgctt
      121 gagtttggga gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacgccaat ggcgaaggca gcttcttgga ccattactga cgttgaggcg cgaaggcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014391             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14557 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014391
VERSION     KHUT01014391.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14557"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           59 c          112 g           36 t
ORIGIN      
        1 gacgtagggg ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcttgg
       61 tgcatccccg gtgaaagccc cccgctcaac gggggagggt ccggggtgat ggccaggctg
      121 gaggcaggga gaggccagtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccggt ggcgaaggcg gctggctgga cctgtcctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014392             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14558 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014392
VERSION     KHUT01014392.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14558"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           56 c          101 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgtgta ggcgggacgg
       61 caagtcggac gtgaaagccc ccggctcaac cggggagggc cgttggaaac tgccgttctt
      121 gagggtcgaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gctctctggt cggcccctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014393             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14559 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014393
VERSION     KHUT01014393.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14559"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           86 g           54 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggattca ctgggcgtaa agcgcacgta ggcggtttgg
       61 taagtctgat gtgaaagccc tcgacttaat cgaggaagtg cattggatac tgtcaaacta
      121 gagtacagga gaggaagatg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaacagt ggcgaaggcg atcttctgga ctgtaactga cgctgaggtg cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014394             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14560 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014394
VERSION     KHUT01014394.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14560"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcatgta ggcggctcgg
       61 aaagtcggat gtgaaagccc agggctcatc cctggaagtg cactcgaaac tcccgagctg
      121 gagtcccgga gaggaaggcg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacacctgt ggcgaaggcg gccttctgga cggtgactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014395             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14561 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014395
VERSION     KHUT01014395.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14561"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           93 g           45 t
ORIGIN      
        1 tacagaggtc tcgagcgtta gtcggaatca ctgggcttaa agcgtacgta ggcggaggtg
       61 caggcgtcgt gtgaaatccc ccggctcaac cggggaatag cacggcgaac cgcacctctt
      121 gagagaggta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggtgaaaaca ggccgctggg ccttctctga cgctgaggta cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014396             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14562 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014396
VERSION     KHUT01014396.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14562"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           53 c          104 g           43 t
ORIGIN      
        1 tacggagagg gcgagcgttg ttcggattta ttgggcgtaa agcgcgtgta ggcggtgcgg
       61 cgagtcggat gttaaagacc cacggctcaa ccggggggat gcgtccgata ctgccgcgct
      121 ggagtgcggg agaggaaagt ggaattccgg gtgtagcggt gaaatgcgta gatatccgga
      181 ggaacaccgg tggcgaaggc ggctttctgg cccgcaactg acgctgagac gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014397             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14563 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014397
VERSION     KHUT01014397.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14563"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           95 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcatgta ggcggtttgt
       61 taagtcgcgt gtgaaagctt ccggctcaac cggaaaagtg cgcgcgaaac tggcgaactt
      121 gagtgccgga gagggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cggacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014398             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14564 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014398
VERSION     KHUT01014398.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14564"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           81 g           55 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttgta
       61 ttagtcttct gtgaaattct tcggcttaac cggggacccg cagaggaaac ggtacaacta
      121 gaggatgcga gaggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca attcactggc gcactcctga cactgaaaca cgaaagcgta
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014399             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14565 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014399
VERSION     KHUT01014399.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14565"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           86 g           53 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatta ttgggcgtac agggtgcgta ggcggtatgt
       61 taagtcaact gttaaattct tcggcctaac cgggggcaag cagtagatac tggcattcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatacctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014400             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14566 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014400
VERSION     KHUT01014400.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14566"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           40 c           78 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ttgggtttaa agggtgcgta ggcggttatt
       61 taagtcagtg gtgaaatcct gtagctcaac tatagaattg ccattgatac tgaataactt
      121 gagtacagac gaggtaggcg gaattgatag tgtagcggtg aaatgcatag atactatcaa
      181 gaacaccgat tgcgaaggca gcttactagg ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014401             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14567 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014401
VERSION     KHUT01014401.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14567"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           96 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggccact
       61 taagctagag gtgaaatccc ggggctcaac cccggaactg ctcctagaac tgggtggcta
      121 gagacaggca gaggcgggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcccgctggg cctgttctga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014402             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14568 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014402
VERSION     KHUT01014402.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14568"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           88 g           53 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggacgtg
       61 taagttagat gtgaaatccc cgagcttaac ttgggaatgg catttaagac tgcatatcta
      121 gagtctgtaa gaggggggta gaattccgag tgtagcagtg aaatgcgtag agattcggag
      181 gaataccagt ggcgaaggcg gccccctggt ataagactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014403             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14569 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014403
VERSION     KHUT01014403.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14569"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           93 g           49 t
ORIGIN      
        1 tacgtaggca ccaagcgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtctgc
       61 caagtcggtt gtgaaatctc ccggcttaac tgggagggtg cgactgaaac tggcaggcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggtgaagacg gctctctgga ccgctactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014404             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14571 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014404
VERSION     KHUT01014404.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14571"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           84 g           52 t
ORIGIN      
        1 tacagaaggt gcaagcgtta atcggaatta ctgggcgtaa agcgcatgta ggcgggtatt
       61 taagtcgatt gtgaaagccc tgggctcaac ctaggaactg cagtcgatac tggataccta
      121 gagtatggta gaggacagtg gaattcccgg tgtagcggtg aaatgcatag atatcgggag
      181 gaacaccagt ggcgaaggcg actgtctgga ttaatactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014405             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14572 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014405
VERSION     KHUT01014405.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14572"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           74 g           61 t
ORIGIN      
        1 tacagagacc tcaagcgtta tccggattta ttgggcgtaa agagcatgta ggcggatttg
       61 ttagtcagat gtcaaatctt cgagctcaac tcgaaaactg catttgaaac ggcaaatctt
      121 gaggaagcga gagaccaata gaacgtatgg tgtagcagtg aaatgcgttg atatcatacg
      181 gaataccaaa ggcgaaggca tttggttggc gcttttctga cgctgagatg cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014406             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14573 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014406
VERSION     KHUT01014406.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14573"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           93 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ttgggcttaa agagcgcgta ggcggcgtcg
       61 taagttggag gtgaaatccc acggctcaac cgtggaactg cccccaatac tgcgatgctt
      121 gagggaggta ggggcctgtg gaacttccgg tggagcggtg aaatgtgttg agatcggaag
      181 gaacgccagc ggcgaaagcg gcaggctgga tctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014407             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14574 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014407
VERSION     KHUT01014407.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14574"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           90 g           54 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggagaa
       61 caagtcagat gtgaaatacc gaagcttaac ttcggggctg catctgaaac tgtttttctt
      121 gagtgtcgga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccagt ggcgaaggcg actttctgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014408             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14575 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014408
VERSION     KHUT01014408.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14575"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           48 c           98 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta ttcggaatta ctgggcgtaa agggcgtgca ggcggaaggg
       61 caagtctggt gtgaaagccc ggggctcaac cccggaagtg cattggaaac tgctcttctt
      121 gagtgctgga gaggaagggg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaatatcagt ggcgaaggcg cccttctgga cggcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014409             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14576 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014409
VERSION     KHUT01014409.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14576"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           94 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaga
       61 taagttggct gtgaaattcc tgggcttaac ctggggcggt cagccaagac tgtcaaactc
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014410             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14577 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014410
VERSION     KHUT01014410.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14577"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           94 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggacgac
       61 caagtcgggg gtgaaatctt tcggctcaac cggaaaatag ccttcgatac tggccgtcta
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaactccggt ggcgaaagcg acacgctgga tctcatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014411             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14578 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014411
VERSION     KHUT01014411.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14578"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           91 g           47 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttgt
       61 caagtcggat gtgaaatccc cgggcttaac ctgggaattg cgttcgaaac tagcaggcta
      121 gagtgcggca gaggggggta gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg tcgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014412             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14579 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014412
VERSION     KHUT01014412.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14579"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgaga
       61 taagtggaat gtgaaagccc cgggcttaac ctgggaagcg cataccaaac gatctgactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014413             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14580 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014413
VERSION     KHUT01014413.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14580"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           87 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggtttga
       61 taagttagtt gtgaaagtcc cgggcttaac ctgggaattg cgactaatac tgtcgagcta
      121 gagtactgta gaggaaggtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg accttctgga cagatactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014414             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14581 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014414
VERSION     KHUT01014414.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14581"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           93 g           48 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgtgcgta ggcggtatgt
       61 taagtctgat gtgaaatccc cgggctcaac ctgggaatgg cattggatac tggcaggcta
      121 gagtacggta gagggtggtg gaattcccgg tgtagcagtg aaatgcgtag agatcgggag
      181 gaacatctgt ggcgaaggcg gccacctgga ccagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014415             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14582 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014415
VERSION     KHUT01014415.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14582"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           89 g           50 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggctcgt
       61 taagtgtaga gtgaaatctc tgggctcacc ccagaaactg ctctgcatac tggcgagctt
      121 gagggtcgca gaggcttgtg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccat ggcgaaggca gcaagctggg cgacatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014416             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14583 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014416
VERSION     KHUT01014416.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14583"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 tacgaggggt gcaagcgtta tccggaatta ttgggcgtac agggtgcgta ggcggcttat
       61 taagtcaatt gttaaatctc ccggcctaac tgggaaccag caattgatac tgacgagcta
      121 gaggatggaa gagagaagcg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014417             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14584 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014417
VERSION     KHUT01014417.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14584"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           93 g           53 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggtttga
       61 tgggtgagat gtgaaagccc tgggcttaac ctgggacgtg cattttaaac tatcaagctg
      121 gaatatatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatattga cgctgaagcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014418             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14585 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014418
VERSION     KHUT01014418.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14585"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           48 c           81 g           48 t
ORIGIN      
        1 tacagagact gcaagcgtta ttcggattca ctgggcgtaa agagtgcgta ggcggtgaga
       61 tgtgtcaggc gtgaaatctc aaggctcaac cttgaaattg cacctgaaac tatttcgcta
      121 gagagctgga gagggtagcg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacaccaat ggcgaaggca gctacctgga cagcaactga cgctaaagca cgaaagcgtg
      241 gggagcaaaa ggg
//
LOCUS       KHUT01014419             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14586 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014419
VERSION     KHUT01014419.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14586"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           52 c           92 g           44 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggtcgcg
       61 caagtcgggt gtgaaatccc ggagcctaac tccggaatgg cattcgaaac tgcgcggctc
      121 gaggtctgga gaggtgactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaggcg agtcactgga cagaacctga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014420             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14587 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014420
VERSION     KHUT01014420.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14587"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           89 g           52 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggtacat
       61 taagtctggt gttaaagact ggcgctcaac gccagcatgg cattggaaac tggtgaacta
      121 gagtgtgtca gaggttacgg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg cgtaactggg gcataactga cgctgaggaa cgaaagccag
      241 gggagcaaaa ggg
//
LOCUS       KHUT01014421             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14588 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014421
VERSION     KHUT01014421.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14588"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           86 g           47 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtgagg
       61 caagttcgat gtgaaatctc cgggctcaac ccggaaatgg cattgaaaac tgccccgcta
      121 gagggttgga tgggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga caacacctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014422             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14589 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014422
VERSION     KHUT01014422.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14589"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           89 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggcggttcag
       61 taagtctttt gtgaaagctc ctggctcaac tgggagaggt caagggaaac tactgggctt
      121 gagtgtggca gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacacctat ggcgaaagca ggcttctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014423             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14590 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014423
VERSION     KHUT01014423.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14590"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           89 g           47 t
ORIGIN      
        1 tacgaaggtc ccaagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggcaggg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacgg catccgatac tgccttgctt
      121 gagtagtgga ggggagcctg gaattcacgg tgtagcagtg aaatgcgtgg atatcgtgag
      181 gaacactcgt ggcgaaggcg aggctctgga cacttactga cgctgagaca cgaaggccag
      241 gggagcaaaa agg
//
LOCUS       KHUT01014424             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14591 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014424
VERSION     KHUT01014424.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14591"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           48 c           81 g           48 t
ORIGIN      
        1 gacaggcaat acaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggtgtcttgc
       61 taagtctggg gtaaaatttc ggggctcaac cccgaaaacg tcctggaaac tcgcaggatt
      121 gagcaatgca gaggtatctg gaatgccgtg agtaggagta aaatccgtag atacacggta
      181 gaacgccgaa agcgaaggca ggatactagg catttgctga cactgagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01014425             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14592 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014425
VERSION     KHUT01014425.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14592"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           86 g           52 t
ORIGIN      
        1 tacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgcc
       61 taagtacctt gtgaaatccc acggcttaac cgtggaactg cttggtatac tgggtgtctt
      121 gagccaacta ggggcagtcg gaacaaacgg tggagcggtg aaatgcgtag atatcgtttg
      181 gaacgccaat ggtgaaaaca gactgctggg gttgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014426             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14593 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014426
VERSION     KHUT01014426.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14593"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           82 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggtccga
       61 taagtcagtg gtgaaagccc gcagctcaac tgcggaattg ccattgaaac tgccggactt
      121 gaatccggtt gaagtgggcg gaatatatca tgtagcggtg aaatgcttag atatgatgta
      181 gaacaccgat tgcgaaggca gctcactaaa ccgacattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014427             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14594 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014427
VERSION     KHUT01014427.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14594"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           93 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggtgcgta ggcggatata
       61 caagtgtgat gtgaaagccc cgggctcaac ctgggaagtg catcgcaaac ggtataactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttaatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014428             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14595 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014428
VERSION     KHUT01014428.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14595"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           87 g           50 t
ORIGIN      
        1 tacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggttttg
       61 caagttagag gtgaaagcct ggagctcaac tccagaacag cctttaaaac tgcatcactt
      121 gaacacggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccgttgttga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014429             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14596 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014429
VERSION     KHUT01014429.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14596"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 tacgggggat gcaagcgtta tccggaatta ttgggcgtaa agcgtccgca ggtggcactt
       61 caagtctgct gtcaaaggtc ggggcttaac cccgggaagg cagtggaaac tgaagcgcta
      121 gagtgtcgta ggggtagagg gaattcccgg tgtagcggtg aaatgcgtag agatcgggaa
      181 gaacatcggt ggcgaaagcg ctctactgga cgacaactga cactcaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01014430             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14597 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014430
VERSION     KHUT01014430.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14597"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           53 c           98 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctgcg gtgaaagcct ccagcttaac tggagaagtg ccgtggatac tggtggtctc
      121 gaggagggta ggggcatctg gaacgttggg tggagcggtg aaatgcgttg atatccaacg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014431             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14598 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014431
VERSION     KHUT01014431.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14598"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           96 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcccac
       61 taagtcggac gtgaaatccc tcggctcaac cggggaactg cgtccgatac tggagggctt
      121 gaattcggga gagggatgcg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgagattga cgctgatgcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014432             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14599 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014432
VERSION     KHUT01014432.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14599"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           93 g           51 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtatgta ggcggttttg
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggag cgccgaaaac tacaaggctt
      121 gagtgcggga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtagacg gctctctgga ccgcgactga cgctgagata cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014433             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14600 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014433
VERSION     KHUT01014433.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14600"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           55 c           93 g           45 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattta ctgggcgtaa agcgtgtgca gacggatggt
       61 caagtggtgt atgaaagcgc tcggctcaac cgggcaatgg tatgccagac tgaccatctc
      121 gagccagaga gagggacgtg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacccccga ggcgaaggcg gcgtcctggc tctggtctga cgttcagaca cgacagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014434             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14601 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014434
VERSION     KHUT01014434.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14601"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           88 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ttgggtgtaa agggtgcgta ggcgggaata
       61 taagtcggat gtgaaatgcc cgggctcaac tcgggagctg cattcgaaac tatatttctt
      121 gagtgaagta gaggcaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttgctggg cttttactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014435             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14602 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014435
VERSION     KHUT01014435.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14602"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           39 c           82 g           58 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggaatta ttgggcgtaa agggtgcgta ggcggcagga
       61 taagttagat gtgaaatatc tgtgcttaac acagaaattg catttaagac tgtcttgctt
      121 gagttctaga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaagcg agtctctggc tagaaactga cgctgaggca cgaaagctgg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014436             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14603 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014436
VERSION     KHUT01014436.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14603"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           59 c           90 g           43 t
ORIGIN      
        1 tacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgcgtgta ggtggacggg
       61 tcagtacctc gtgaaatccc acggctcaac cgtggaactg ccgggtatac tgcccgcctt
      121 gaggcactca ggggcgagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgcccat ggtgaaaaca gctcgctggg ggtgtcctga cactgagacg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014437             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14604 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014437
VERSION     KHUT01014437.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14604"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          106 g           39 t
ORIGIN      
        1 tacggaaggg gcaagcgttg ttcggcatca ttgggcgtaa aggagtgagt aggcggtctg
       61 gtaggtctgc tgtgaaagcc cacggcttcc ccgtggaagg gcagcggaaa cggccggact
      121 tgaggctggg aggggtgggt ggaattcccc gtgtagcggt gaaatgcgta gagacggggg
      181 agaacactgg tagcgaaggc ggccagctgg accagccctg acgctgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014438             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14605 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014438
VERSION     KHUT01014438.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14605"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           51 c           93 g           43 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcctcg
       61 caggtcggat gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac cgcaaggctc
      121 gagtcccgga gaggaaggtg gaattctagg tgtagaggtg aaattcgtag atatctggag
      181 gaacaccggt ggcgaaagcg gccttctgga cgaggactga cgctgagacg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014439             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14606 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014439
VERSION     KHUT01014439.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14606"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 tcagtcggac gtgaaatccc cgggctcaac tcgggaactg cgtccgatac tggaaggctg
      121 gagttcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgaaactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014440             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14607 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014440
VERSION     KHUT01014440.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14607"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 aacgtagggg acaagcgtta tccggattta ctgggcgtaa agcgcccgta ggcggctttt
       61 taagttggac gtgaaagctc ccggcttaac tgggagaggc cgttcaaaac tggaaagctc
      121 gagtgaggaa gaggagagca gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaatgccagt ggcgaaagcg gctctctggt cctaaactga cgctgagggg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014441             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14608 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014441
VERSION     KHUT01014441.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14608"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           83 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtac agggcgcgta ggcgggaata
       61 gaagtcagat gtgaaatgcc ggagctcaac tccggaactg catttgaaac tctatttctt
      121 gaggctagtc ggagaaagcg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gctttctagg ttagacctga cgctgatgcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014442             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14609 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014442
VERSION     KHUT01014442.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14609"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          100 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtccgcg gtgaaatcct ccggcttaac cggagaagtg ccgcggatac tgggggcctc
      121 gagggaggta ggggcgtgcg gaacactggg tggagcggtg aaatgcgttg atatccagtg
      181 gaactccggt ggcgaaggcg gcacgctgga cctcatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014443             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14610 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014443
VERSION     KHUT01014443.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14610"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           85 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtctgat gtgaaatctc cgagcttaac tcggaaatgg cattggatac taccttgcta
      121 gaggattgga ggggagactg gaattcttgg tgtagcagtg aaatgcgtag atatcaagag
      181 gaacaccagt ggcgaaggcg agtctctgga caattcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014444             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14611 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014444
VERSION     KHUT01014444.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14611"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggtgcgg
       61 taagttgggt gtgaaatccc tgggcttaac ccaggaatgg cgcctaatac tgccggactc
      121 gagggagaca gaggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccagt ggcgaaagcg gcttactggg tctcttctga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014445             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14612 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014445
VERSION     KHUT01014445.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14612"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           89 g           50 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcccgt
       61 taagttggtt gtgaaatccc acggctcaac cgtggaagtg caaccaaaac tggcgggctt
      121 gagtcctgaa gagaatggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg gccatttggg aaggtactga cgctgagacg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014446             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14613 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014446
VERSION     KHUT01014446.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14613"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c           94 g           47 t
ORIGIN      
        1 tacgaagggc ccgagcgttg cgcggaatta ctgggcttaa agcgtgcgca ggcgggcatg
       61 caggcgcttt gtgaaatccc tcgactcaat cgaggaattg cttggcgaac cgcatgtctt
      121 gaggcaagta ggggcggtcg gaacgatagg tggagtggtg aaatgcgttg atatctatcg
      181 gaacgccgaa ggagaaatca ggccgctggg cttgtcctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014447             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14614 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014447
VERSION     KHUT01014447.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14614"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           90 g           53 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattca ctgggcgtaa agcgagtgca ggtggttgtg
       61 taagttggat gtgaaatttc ccggcttaac cgggaagggt cattcaatac tgcgcgactt
      121 gagggcagaa gaggggggtg gaattgccgg tgtagtggtg aaatgcgtag atatcggcag
      181 gaacacctgt ggcgaaagcg gccccctggt ctgctcctga cactcagact cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014448             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14615 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014448
VERSION     KHUT01014448.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14615"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           47 c           91 g           53 t
ORIGIN      
        1 tacagaggtt ccaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggcggcgctg
       61 taagtcggat gtgaaatctt agggctcaac cctgaaattg cattcgatac tgtagtgctc
      121 gaggactgga gaggagactg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt ggcgaaggcg ggtctctgga cagttcctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014449             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14616 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014449
VERSION     KHUT01014449.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14616"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           87 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggccagg
       61 taagtttggt gtgaaagctc ggagctcaac tccgaaatgt cattgaatac tatctggctg
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgaaggcg agtctctgga cgaatcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014450             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14617 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014450
VERSION     KHUT01014450.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14617"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcggctttc
       61 taagtcaggg gtgaaatcct ggagctcaac tccagaactg cctttgatac tggagagctt
      121 gagtccggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014451             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14618 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014451
VERSION     KHUT01014451.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14618"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcaatg
       61 taagtcagac gtgaaatcct tcggcttaac tgaagaactg cgtctgaaac tgcatggcta
      121 gagtgcagaa gagggaaacg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gtttcctggt ctgacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014452             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14619 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014452
VERSION     KHUT01014452.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14619"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           89 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatagc
       61 taagtcagat gtgaaatccc cgagctcaac tcgggaactg catttgaaac tggcagtcta
      121 gagtgctgga gagggaagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttcctgga cagtaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014453             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14620 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014453
VERSION     KHUT01014453.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14620"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           55 c          100 g           43 t
ORIGIN      
        1 tacgtagggg gccagcgttg tccggattta ctgggcgtaa agcgcgcgta ggcggaacgt
       61 taagtctggc ctgaaagccc ccggctcaac cggggaaggt ggctggaaac tggcgttctt
      121 gagggcgata gaggagtgtg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacacccat ggcgaaggca gcactctggg tcggccctga cgctgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014454             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14621 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014454
VERSION     KHUT01014454.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14621"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           93 g           49 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtttcg
       61 taagttcggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcgaagctc
      121 gagtatggga gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccagc ggtgtagacg gcttgctaga ccatgactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014455             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14622 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014455
VERSION     KHUT01014455.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14622"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           94 g           49 t
ORIGIN      
        1 gacggaggga gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggttttt
       61 taagtgaggc gtgaaagccc tgggcttaac ccaggaggtg cgcttcagac tggaaagctt
      121 gaggacgaga gaggaaagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg actttctggc tcgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014456             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14623 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014456
VERSION     KHUT01014456.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14623"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           84 g           53 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agggcaggta ggtggtttaa
       61 taagtccgtt gtgtcagctc ctggcttaac tgggagaggt caatggaaac tattagactt
      121 gagtatagga gagggtactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaggca ggtacctgga ctattactga cactcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014457             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14624 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014457
VERSION     KHUT01014457.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14624"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           92 g           49 t
ORIGIN      
        1 tacggaggga gcaagcgtta ctcggaatta ctgggcgtaa agggcgcgta ggcgattcat
       61 taagtcttgt gtgaaagccc atggcttaac tgtggaagtg cacgagaaac tgatgggttt
      121 gagtacagga gaggaaggca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gccttctgga ctgtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014458             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14625 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014458
VERSION     KHUT01014458.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14625"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           83 g           54 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtttgg
       61 taagttcgat gtgaaatctc cgagcttaac ttggaaactg cattgaaaac tattgaactg
      121 gaggattgga ggggggactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga caaatcctga cactgaggca cgaaagccag
      241 gggagcaaac agg
//
LOCUS       KHUT01014459             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14626 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014459
VERSION     KHUT01014459.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14626"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           62 c           95 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcgggcgac
       61 caagtcaagg gtgaaatccc ccggcccaac cggggaactg cctctgatac tggtcgtctc
      121 gagggggata ggggcacgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga tcccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014460             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14627 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014460
VERSION     KHUT01014460.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14627"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtgaag
       61 taagtaagat gtgaaatccc tgggcttaac ctgggaaggt catcttaaac tgcttgactg
      121 gagtacggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ctgatactga cactgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014461             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14628 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014461
VERSION     KHUT01014461.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14628"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           94 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggccagg
       61 taggtccgtt gtgaaaactt gaggctcaac ttcgagatgt cgacggaaac catctggcta
      121 gagtccggaa gaggagagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacacccgt ggcgaaagcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014462             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14629 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014462
VERSION     KHUT01014462.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14629"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           46 c           97 g           49 t
ORIGIN      
        1 gacgaagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcttg
       61 ttagtcaggc gtgaaagccc tgggcttaac ctaggatgtg cgcttgatac tgcgaggctt
      121 gagtgtggga gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gccaactgga ccacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014463             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14630 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014463
VERSION     KHUT01014463.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14630"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggcggatggg
       61 tgtgtcaggt gtgaaatccc caggctcaac ttgggaactg cacttgaaac tgcccgtctc
      121 gagtggtgga gaggagagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctctctgga caccgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014464             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14631 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014464
VERSION     KHUT01014464.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14631"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           91 g           52 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcgggtcgt
       61 taagtcaggg gtgaaatccc ggagctcaac tccggaactg cctttgatac tggcgacctt
      121 gaggccggaa gaggttagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacacctgt ggcgaaggcg gctaactggt ccggatctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014465             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14632 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014465
VERSION     KHUT01014465.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14632"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           84 g           55 t
ORIGIN      
        1 tacagaggcc ccgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggtcgtg
       61 taagtcagat gtgaaagccc caggcttaac ctgggaagtg catttgatac tgcacggctg
      121 gagtgcgaaa gaggaaagcg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaataccgat ggcgaaggca gctttctggt tcgtaactga cactgagatt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014466             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14633 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014466
VERSION     KHUT01014466.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14633"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           42 c           83 g           50 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaataa ctgggtgtaa agagcgtgaa ggcggtttgt
       61 taagtcaatt gttaaagagc ccagcttaac tgggcaaaag caagtgaaac tggcagacta
      121 gagtacagaa gagaagagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccggt tgcgaaggcg gctctttggt ctgtaactga cgctcaaacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014467             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14634 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014467
VERSION     KHUT01014467.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14634"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           79 g           55 t
ORIGIN      
        1 tacgaaggga gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggcggataag
       61 aaagtcagaa gtgaaatccc aaggcttaac catggaattg cttttgaaac ttcttatctg
      121 gagtatggta gaggatagcg gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaacaccagt tgcgaaggcg gctatctgga ccattactga cgctgaggta cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01014468             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14635 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014468
VERSION     KHUT01014468.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14635"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           84 g           49 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgaacgca ggcggatggt
       61 taagtaggaa gtgaaaggtg ggagctcaac tcctacactg ctccctatac tggccgtctt
      121 gagtagcgga gaggaagagg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaagca ctcttctgga cgcagactga cgctgaggtt cgaaagccaa
      241 ggtagcaaac agg
//
LOCUS       KHUT01014469             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14636 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014469
VERSION     KHUT01014469.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14636"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           95 g           44 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggcggctggc
       61 tcagtcagac gtgaaatccc tcggctcaac cgaggaactg cgtctgatac tagccagctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggc ggcgaaggcg gcatcctgga ccggtattga cgctgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014470             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14637 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014470
VERSION     KHUT01014470.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14637"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          103 g           37 t
ORIGIN      
        1 gacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggtcgcg
       61 caggtcatct gtgaaagcac ccggctcaac cgggctgatg cgggtgaaac cgcgcgactt
      121 gagggcggga gaggtcggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacccgt agcgaaggcg gccgactgga ccgatcctga cgctgaggcg cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014471             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14638 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014471
VERSION     KHUT01014471.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14638"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           92 g           51 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggattga ctgggcgtaa agcgcacgta ggcgggtttt
       61 taagtcggct gtgaaagccc cgggcttaac ctgggaactg cagccgaaac tgaaaatctt
      121 gaatgcttga gaggttggtg gaattcctgg tgtaggagtg aaatccgtag agatcaggag
      181 gaacatcggt ggcgaaggcg accaactggc gatgcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014472             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14639 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014472
VERSION     KHUT01014472.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14639"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           94 g           50 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agagcgtgta ggcggatatt
       61 taagtcaggt gtgaaagacc ggggctcaac cccgggattg cacttgatac tgggtatctt
      121 gagggcagga gaggaaagta gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaataccggt ggcgaaggcg gctttctgga ctgaccctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014473             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14640 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014473
VERSION     KHUT01014473.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14640"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           40 c           83 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agcgtgcgta ggtggttagg
       61 taagttagat gtgaaatccc cgggcttaac ctgggaactg cgtttaaaac tgtttaacta
      121 gagtactgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014474             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14641 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014474
VERSION     KHUT01014474.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14641"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           39 c           85 g           57 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttata
       61 taagttaggt gtgaaatttc tgggcttaac ctggaaactg cgcctaatac tgtgtgacta
      121 gagttctgga gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaagcg actttctggc tagaaactga cactgaggca cgaaggcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014475             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14642 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014475
VERSION     KHUT01014475.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14642"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           54 c           91 g           45 t
ORIGIN      
        1 aacgtaggat ccgagcgtta tccgaattca ctgggcgtaa agcgcgtgca ggcggccgga
       61 aaagttggat gtgaaatctc ctggctcaac tgggagaggt cgttcaagac tatctggctc
      121 gaggacggta gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctggg ccgatcctga cgctcagacg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014476             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14643 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014476
VERSION     KHUT01014476.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14643"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggtcggg
       61 taagtctgat gtgaaatctc cgggctcaac ccggaaaagg catcggatac tacttggctg
      121 gagggttgga ggggggactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaat ggcgaaggct agtccctgga caacttctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014477             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14644 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014477
VERSION     KHUT01014477.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14644"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c           92 g           48 t
ORIGIN      
        1 tacgtaggtg acaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggttcga
       61 tcagtcgaat gtgaaatccc ccggctcaac tggggtgggt cattcgaaac agtcggactc
      121 gaaggcagga gagggtagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg actacctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014478             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14645 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014478
VERSION     KHUT01014478.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14645"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatgtc
       61 taagtacctt gtgaaatccc atagctcaac tatggaactg ctcggtatac tggacgtctt
      121 gagccactta ggggctaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggtagctggg agtgtgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014479             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14646 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014479
VERSION     KHUT01014479.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14646"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           90 g           45 t
ORIGIN      
        1 gacagaggat gcaaacgttg ctcggaatta ctgggcgtaa agcgcgcgca ggcggtcttg
       61 taagtcaggt gtgaaatccc ggggctcaac ctcggaagtg cacttgatac tgctcgactg
      121 gagtactgga gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctgga cagatactga cgctgaggcg cgaaagcgtg
      241 ggaagcaaac agg
//
LOCUS       KHUT01014480             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14647 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014480
VERSION     KHUT01014480.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14647"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           75 g           59 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgca ggcggaatga
       61 taagtcagtg gtgaaatcca acagcttaac tgttgaactg ccattgatac tgtcgttctt
      121 gagtatagtt ggagtgggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat cgcgaaggca gctcgctaaa ctaaaactga cgctcatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014481             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14648 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014481
VERSION     KHUT01014481.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14648"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           35 c           74 g           70 t
ORIGIN      
        1 tacggaaggt gcaagcgtta tccggtttta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtt gtgaaatcct agtgcttaac gctagaactg caattgatac tggtaggctt
      121 gagttaagaa gaggtaagta gaatttatgg tgtagtagtg aaatgcttag atatcataag
      181 gaataccaat agcgtaggca gcttactggt ctttaactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014482             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14649 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014482
VERSION     KHUT01014482.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14649"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           45 c           95 g           52 t
ORIGIN      
        1 tacgtaggca gcgagcgttg ttcggagtta ctgggcgtaa agggtgtgta ggcggttctc
       61 taagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaatac tgaagagcta
      121 gagtgcggga gaggagagtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggc ggtgtaaacg gctctctgga ccgtgactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014483             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14650 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014483
VERSION     KHUT01014483.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14650"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           86 g           57 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatca ttgggcgtaa agcgcatgta ggtggctatg
       61 taagtcagat gtgaaatccc tgggcttaac ccaggacgtg catttgatac tgcgtagctt
      121 gaatatggga gagggtagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctggc ccaatattga cactgagatg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014484             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14651 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014484
VERSION     KHUT01014484.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14651"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           90 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtctag
       61 caagtcgggt gtgaaatccc ttggctcaac caaggaactg cattcgaaac tgcttaacta
      121 gagtgtcgga gggggtggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacacctgt ggcgaaggcg gccacctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014485             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14652 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014485
VERSION     KHUT01014485.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14652"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          102 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgc
       61 cgagtcaggg gtgaaatcgt tcggcttaac cggacaagtg cctctgatac tggcggcctc
      121 gaggggggta ggggcctgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgggctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014486             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14653 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014486
VERSION     KHUT01014486.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14653"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           49 c           94 g           49 t
ORIGIN      
        1 aacgtaggtg gcgagcgtta tccggattta ctgggcgtaa agagcttgta ggcggtcttg
       61 taagttgggc gtgaaagccc cgggcttaac tcggggaggc cgttcaatac tgcagggcta
      121 gaggatgtca gagggagatg gaatacctgg tgtagcagtg aaatgcgtag atatcaggtg
      181 gaacacccgt ggcgaaggcg gtctcctggg gcactcctga cgctgagaag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014487             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14654 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014487
VERSION     KHUT01014487.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14654"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           95 g           46 t
ORIGIN      
        1 aacgtaggag gcaagcgtta tccgaattta ctgggcgtaa agcgtgtgca ggcggttcga
       61 taagttgggt gtgaaaactc ctggctcaac tgggagaggt cgctcaagac tgtcggactg
      121 gagggcagga gagggaggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gcctcctgga ctgtacctga cgctgagaca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014488             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14655 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014488
VERSION     KHUT01014488.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14655"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           88 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcaggta ggtggtcttg
       61 taagtctagt gtgaaatccc cgagctcaac ttgggaactg cgctggatac tgcaagacta
      121 gagtgctgga ggggtgcgta gaattctcgg tgtagcggtg aaatgcgtag atatcgagag
      181 gaataccaga ggcgaaggcg gcgcactaga cagcaactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014489             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14656 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014489
VERSION     KHUT01014489.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14656"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           92 g           43 t
ORIGIN      
        1 gacagagggt gcgaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcatcg
       61 caagtcggat gtgaaagccc ggggctcaac cccggaagtg cattcgatac tgcgaagcta
      121 gagtctcgga gaggatggtg gaactctcgg tgtagaggtg aaattcgtag atatcgagaa
      181 gaacaccggc ggcgaaggcg gccatctgga cgatgactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014490             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14657 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014490
VERSION     KHUT01014490.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14657"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           59 c           99 g           39 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggattca ctgggcataa agggcgcgta ggcggttccg
       61 aaggtggcgc gtgaaatccc ccggctcaac cggggaacgg cgcgccaaac cgcggttctc
      121 gagggcggta ggggggactg gaactcttgg tggagcggtg aaatgcgtag atatcaagag
      181 gaacaccggt ggcgaaggcg agtccctggt ccgcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014491             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14658 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014491
VERSION     KHUT01014491.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14658"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           56 c          100 g           43 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggccccg
       61 atagtcaggc gtgaaattcc tgggctcaac ctggggactg cgcttgatac gtcggggctg
      121 gagtgcggga gagggtcgcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggc ccgttactga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014492             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14659 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014492
VERSION     KHUT01014492.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14659"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggaagta
       61 caagtcaggt gtgaaatttt ggggctcaac tccagagcgg cacttgaaac tgtacatcta
      121 gagggatggc ggaggaaatg gaattccacg tgtagcggtg aaatgcgtag atatgtggag
      181 gaacacctgt ggcgaaagcg gttttctagc ttttacctga cgctgaggcg cgagagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014493             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14660 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014493
VERSION     KHUT01014493.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14660"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatca ttgggcgtaa agagggcgta ggtggctctg
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cattcgatac tgcggagctt
      121 gagtgttgga gaggttatcg gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaacaccgga ggcgaaggcg ggtaactggc caaacactga cactgaggcc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014494             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14661 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014494
VERSION     KHUT01014494.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14661"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           86 g           56 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtccgca ggcggattga
       61 taagtcagtg gtgaaatctc agtgcttaac attggggctg ccattgatac tgttagtctt
      121 gaggatggat gaagtgggcg gaatacagca tgtagcggtg aaatgcgtag agatgctgta
      181 gaacaccgat tgcgaaggca gctcactaaa ccatgactga cgctgaggga cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014495             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14662 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014495
VERSION     KHUT01014495.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14662"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           45 c           77 g           61 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggactgg
       61 taagtccgtg gtgaaatccc cgagcttaac ttgggaactg ccatggatac tattagtctt
      121 gaatatcgtg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca cgtttattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014496             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14663 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014496
VERSION     KHUT01014496.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14663"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           77 g           62 t
ORIGIN      
        1 tacgaagggt ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtg gtgaaagcct tcagcttaac tgaagaactg ccattgatac tgatgagctt
      121 gagtatagtt gaggttggcg gaatgtgtca tgtagcggtg aaatgcttag atatgacaca
      181 gaacaccgat tgcgaaggca gctagctaag ctataactga cgctgatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014497             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14664 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014497
VERSION     KHUT01014497.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14664"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           92 g           47 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggtggccaaa
       61 taagtcggat gtgaaagccc agggcttaac cctggaagtg catccgatac tgtatggctt
      121 gagtcccgga gaggttcgtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg acgaactgga cgaggactga cactgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014498             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14665 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014498
VERSION     KHUT01014498.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14665"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           92 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggtccgt
       61 taagtcgggt gtgaaatctc cgagctcaac tcggaaactg cacccgatac tgtcggactt
      121 gaggacggta gaggtcggtg gaatgaccag tgtaccggtg aaatggacag atattggtcg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgttcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01014499             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14666 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014499
VERSION     KHUT01014499.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14666"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           75 g           61 t
ORIGIN      
        1 tacgaaggat cctagcgttg tccggattta ctgggtttaa agggtgcgta ggcggacttt
       61 taagtcagtg gtgaaagctt gcagcttaac tgtaaaattg ccattgaaac tgagagtctc
      121 gagtacggtt gaggtcactg gaatgtatca tgtagcggtg aaatgcttag atatgataca
      181 gaacaccaat tgcgaaggca ggtgactaaa ccgtaactga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014500             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14667 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014500
VERSION     KHUT01014500.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14667"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           91 g           44 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgtacgca ggcggtcttg
       61 cgagtcgggc gtgaaagtcc ggggctcaac cccgggaagt cgtccgatac tacgagactt
      121 gagtacggga gaggggtgtg gaattcccag tgtagaagtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg acaccctggc ccgaaactga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014501             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14668 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014501
VERSION     KHUT01014501.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14668"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c           99 g           50 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agggcgtgca ggcggtctgt
       61 taagtctagg gtgaaagctc ccggctcaac tgggagaggt ccttggatac tggcggactt
      121 gagggagaca gaggagagag gaattcccgg tgtagtggtg atatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg tcttacctga cgctgagacg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014502             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14669 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014502
VERSION     KHUT01014502.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14669"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           50 c           84 g           48 t
ORIGIN      
        1 tacgaggggt ccaagcgttg ttcggaatca ctgggcgtaa agcgggtgta ggtggccaag
       61 caagttggat gtgaaagccc caggctcaac ctgggaagtg catccaaaac tacatggctt
      121 gagtaaggga gagaaaggta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaacaccggt ggcgaaggcg gccttttggc ccattactga cactcagacc cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014503             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14670 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014503
VERSION     KHUT01014503.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14670"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           88 g           52 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agggttcgta ggtgggaagg
       61 taagtcgagt gtgaaatccc tgagcttaac tcaggacgtg cacccgaaac tgcttttctt
      121 gagtgttgga gagggtcgta gaattgctgg tgtaggagtg aaatccgtag atatcagcag
      181 gaataccaga ggcgaaggcg gcgacctggc caaacactga cactgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014504             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14671 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014504
VERSION     KHUT01014504.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14671"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           89 g           51 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttgat
       61 taagttatct gtgaaatacc caggcttaac ctgggcaggt cagataatac tggacaacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014505             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14672 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014505
VERSION     KHUT01014505.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14672"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           50 c           96 g           46 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agagcgcgta ggcggctcgg
       61 taagtctgtt gtgaaagtca agggctcaac ccttgaatgc caatggatac tgtcgagcta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014506             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14673 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014506
VERSION     KHUT01014506.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14673"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           84 g           53 t
ORIGIN      
        1 tacagaaggt gcgagcgtta atcggaatta ctgggcgtaa agagtgcgta ggtggtttat
       61 taagtcaggc gtgaaatcca cgggctcaac ccgtgaactg cgtttgaaac tggtaggcta
      121 gagtatggta gagggaagtg gaatttcagg tgtagcggtg aaatgcgtag atatctgaag
      181 gaacaccagt ggcgaaggcg acttcctggc ccaatactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014507             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14674 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014507
VERSION     KHUT01014507.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14674"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           59 c          108 g           34 t
ORIGIN      
        1 tacgtaggag acgagcgttg tccggagtta ctgggcgtaa agggtgcgca ggcggctgcg
       61 cgcgtgtgcg gtgaaatctc ccggctcaac tgggaggggg ccgtgcagac ggcgcagctt
      121 gagggtcgga gaggggcgtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgac ggcgaaggca gcgccctggc cgacgactga cgctcaggca cgaaagctgg
      241 gggagcgaac agg
//
LOCUS       KHUT01014508             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14675 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014508
VERSION     KHUT01014508.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14675"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           43 c           89 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatta ctgggtgtaa agggagcgta gacggttacg
       61 taagtctgga gtgaaaggca agggcccaac ccttggactg ctctggaaac tatgaaacta
      121 gagtgcagga gaggtaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga ctgtaactga cgttgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014509             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14676 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014509
VERSION     KHUT01014509.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14676"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           55 c          104 g           43 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcacgta ggtggctgcc
       61 taagatgggc gtgaaatctc cgggctcacc tcggaggggt cgtccgtgac tgggtagctc
      121 gggtctggta gaggcaggtg gaattcccgg tggagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg acctgctggg ccagtaccga cgctgaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014510             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14677 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014510
VERSION     KHUT01014510.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14677"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           41 c           78 g           59 t
ORIGIN      
        1 gacgaaggat gcaagcgtta tccggattca ttgggtttaa agggtgcgta ggcggtttat
       61 taagtcagtg gtgaaagccg gtcgcttaac gatcgaattg ccattgatac tggtagactt
      121 gagtacagat gctgtgggcg gaatatgaca tgtagtggtg aaatacatag agatgtcata
      181 gaacaccgat tgcgaaggca gctcacaaaa ctgtaactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014511             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14678 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014511
VERSION     KHUT01014511.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14678"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           93 g           47 t
ORIGIN      
        1 gacgtagggt ccgagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggtccgt
       61 taagtgagga gtgaaagctc cgggctcaac ccggaaatcg cttttcatac tggcggactc
      121 gagggtggca gaggcttgtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacacccat ggcgaaggca gcaagctggg ccgcatctga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014512             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14679 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014512
VERSION     KHUT01014512.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14679"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagttcgta ggcggtttac
       61 caagtctgac gttaaagccc agcgcttaac gctggtatgg catcggaaac tggtaggcta
      121 gagtctggta gaggcaaggg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ccttgctggg ccagtactga cgctgaggaa cgaaagctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01014513             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14680 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014513
VERSION     KHUT01014513.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14680"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           95 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctgag gtgaaagcct tgggcttaac ccaagaagtg cctcggaaac ggtgagactt
      121 gagtcctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcggcctgga caggaactga cactgaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014514             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14681 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014514
VERSION     KHUT01014514.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14681"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           93 g           50 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ctgggcttaa agagcacgta ggcggccctt
       61 caagtgtctt gtgaaatccc tcggctcaac cgaggaattg ctgggcatac tgtcgggctt
      121 gaggtaggta tgggcgggtg gaactcttgg tggagcggtg gaatgcgtag atatcaagag
      181 gaacgccggt ggtgaagacg atccgctagg cctatcctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014515             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14682 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014515
VERSION     KHUT01014515.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14682"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           92 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtcttg
       61 taagtcgggt gtgaaagccc agggctcaac cctggaagtg cactcgaaac tgcaaggcta
      121 gagtaccgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgagtactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014516             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14683 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014516
VERSION     KHUT01014516.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14683"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggcgcg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tgtgtgtcta
      121 gagggtaggc ggagaaaaca gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggcgaaggcg gttttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014517             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14684 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014517
VERSION     KHUT01014517.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14684"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           52 c          105 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatta ctgggtgtaa agcgcgtgta ggcggctcgt
       61 taagtttgtg gtgaaagcgc ccggctcaac cgggcagagt ccacagaaac tggcgggctg
      121 gaggtcggta gaggcgagtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcgctggg ccggacctga cgctgagacg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014518             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14685 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014518
VERSION     KHUT01014518.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14685"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           96 g           44 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatca ctgggcgtaa agcgcgcgta ggcggcctgt
       61 taagtcagat gtgaaagccc cgggctcaac ctgggaaccg catttgatac tggcgggctc
      121 gagtccgaaa gaggggggta gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gccccctggt tcgagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014519             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14686 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014519
VERSION     KHUT01014519.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14686"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacgtagggt ccgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggtggttttg
       61 taagccagat gtgaaatccc cgggcttaac ctgggaactg catttggtac tgcaaggcta
      121 gagtacggca gagggaggtg gaattccagg tgtagcagtg aaatgcgtag agatctggag
      181 gaacaccgat ggcgaaggca gcctcctggg ctagtactga cgctcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014520             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14687 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014520
VERSION     KHUT01014520.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14687"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           89 g           46 t
ORIGIN      
        1 tacggaggga gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggccatt
       61 caagtcagag gtgaaagccc agggctcaac cctggaatag cctttgaaac tagatggctc
      121 gaatccggga gaggtgagtg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gctcactgga ccggcattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014521             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14688 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014521
VERSION     KHUT01014521.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14688"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           59 c           94 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgcgtgta ggcggtccgc
       61 aaagtcggtt gtgaaatctc cgagctcaac tcggaaactg cgaccgatac ttgcggactt
      121 gaggacggta gaggtcggtg gaatgtgcgg tgtaccggtg aaatgggtaa atatcgcacg
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacg cgaaagccag
      241 gggagcgaac cgg
//
LOCUS       KHUT01014522             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14689 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014522
VERSION     KHUT01014522.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14689"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           44 c           92 g           53 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggattta ctgggtgtaa agggtgcgta ggcgggactg
       61 caagtcagac gtgaaatgcc aaggcttaac cttggagctg cgtttgaaac tgtggttctt
      121 gagtgaagta gaggcaggcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcctgctggg ctttaactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014523             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14690 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014523
VERSION     KHUT01014523.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14690"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           48 c           95 g           51 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccatg
       61 taagtcgggt gtgaaatccc aaggctcaac cttggaactg cattcgatac tgtgtggctt
      121 gagaccggaa gaggtgggtg gaatacccag tgtagaggtg aaattcgtag atattgggtg
      181 gaacaccggt ggcgaaggcg gctcactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014524             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14691 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014524
VERSION     KHUT01014524.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14691"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           97 g           41 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggaatca ctgggcataa agcgcgcgta ggcggctaag
       61 taagtcgggt gtgaaatccc ccagctcaac tggggaactg cgcccgatac tgcttggctt
      121 gaggtccgga ggggagagtg gaattcccgg tggagcggtg aaatgcgtag agatcgggaa
      181 gaacgccggc ggcgaaagcg actctctggt cggatcctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014525             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14692 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014525
VERSION     KHUT01014525.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14692"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           89 g           47 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcggtctcg
       61 caagtcggat gtgaaagccc caggctcaac ctgggaactg catccgaaac tgcaagactt
      121 gaatgcttga gaggttggtg gaattccagg tgtaggagtg aaatccgtag atatctggag
      181 gaacatcggt ggcgaaggcg accaactggt gatgcattga cgctgaggtg cgaaagcgcg
      241 gggagcaaac agg
//
LOCUS       KHUT01014526             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14693 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014526
VERSION     KHUT01014526.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14693"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           89 g           49 t
ORIGIN      
        1 tacgtatgga gcaagcgtta tccggattta ctgggtgtaa agggagcgca ggcggtctgg
       61 caagtctgat gtgaaaaccc ggggctcaac cccgggactg cattggaaac tgtcagacta
      121 gagtgtcgga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014527             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14694 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014527
VERSION     KHUT01014527.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14694"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggccatc
       61 taagtctggt gtgaaagccc ggggcctaac cccggaagtg cactggatac tggatggcta
      121 gaggacgaga gaggagattg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatcagt ggcgaaggcg aatctctggc tcgtatctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014528             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14695 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014528
VERSION     KHUT01014528.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14695"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           95 g           44 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggcggtgagg
       61 tgtgtctgac gtgaaagccc tcggctcaac cggggaattg catcggaaac tacctcgctg
      121 gaatgcagga gagggtggcg gaattcctgg tgtagaggtg aaattcgtag ataccaggag
      181 gaacaccgga ggcgaaggcg gccacctggc ctgtgattga cgctgaggca caaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014529             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14696 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014529
VERSION     KHUT01014529.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14696"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           96 g           45 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtctgc
       61 caagtcgggg ttgaaatctt tcggctcaac cggaaaagcg gcttcgaaac tggcaggctg
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgtgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014530             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14697 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014530
VERSION     KHUT01014530.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14697"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           46 c           84 g           50 t
ORIGIN      
        1 tacagaggtg ccaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggctata
       61 caagtcgggt gtgaaatacc ggagcctaac ttcggaacgg cattcgaaac tgtatagctc
      121 gagatctgca gaggtgattg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccaga ggcgaaagcg aatcactgga cagaatctga cgctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014531             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14698 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014531
VERSION     KHUT01014531.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14698"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           98 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtccgt
       61 taagtcagcc gtgaaagccc cgggcttaac ctgggaactg cggatgatac tggcgggcta
      121 gagtgtggca gagggtggcg gaatttccgg tgtagcagtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gccatctggg ctaacactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014532             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14699 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014532
VERSION     KHUT01014532.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14699"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacggaaggt gcaagcgttg ttcggaatga ctgggcgtaa agcgcacgta ggcgggttcg
       61 taagtcggat gtgaaagccc tgggctcaac ctgggaactg cacccgaaac tccgaatctt
      121 gaatgcctga gaggttggcg gaattcctgg tgtaggagtg aaatccgtag atatcaggag
      181 gaacatcgga ggcgaaggcg gccaactggt ggtgcattga cgctaaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014533             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14700 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014533
VERSION     KHUT01014533.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14700"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           43 c           81 g           53 t
ORIGIN      
        1 tacgaagggt gcaagcgtta ttcggaatga ttgggcgtaa agggtgcgta gacggtgcat
       61 taagtcaact gttaaatttc ctggcctaac taggagcaag cagtagaaac tagtgtgctc
      121 gaggatggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccatatctga cgttcaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014534             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14701 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014534
VERSION     KHUT01014534.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14701"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           48 c           82 g           50 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ttgggcgtat agggcgcgta ggcgggcatg
       61 taagtcggac gtgaaatccc agggctcaac cctggaacag catccgaaac tgtatgtcta
      121 gaggatagga ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacccgt ggcgaaggcg gttttctatc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014535             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14702 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014535
VERSION     KHUT01014535.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14702"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           93 g           47 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtccgag gtgaaatcct tgggcttaac ctgagaaggg cctcggaaac ggtaagacta
      121 gagttctgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga caggcactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014536             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14703 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014536
VERSION     KHUT01014536.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14703"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           43 c           87 g           53 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggctatg
       61 taagtcagac gtaaaatacc taagcttaac ttagggcgtg cgtttgatac tgcgtagcta
      121 gagaatggga gaggagagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctctctggc ccattactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014537             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14704 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014537
VERSION     KHUT01014537.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14704"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gcaaacgttg ctcggaatca ctgggcataa agcgcacgta ggtggcatgg
       61 taagtctgat gtgaaatccc tcggctcaac cgaggaactg cgttggatac tgctgtgctt
      121 gagggttgga ggggagattg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg aatctctgga caacttctga cactgaggtg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014538             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14705 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014538
VERSION     KHUT01014538.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14705"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           63 c          104 g           35 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggg
       61 ccagtccggg gtgaaagccc ccggctcaac cggggaacgg ccttggatac tgcctgtctg
      121 gaggggggta ggggcacgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg gcgtgctgga ccccgcctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014539             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14706 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014539
VERSION     KHUT01014539.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14706"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           58 c           96 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggctcg
       61 ttagtcgggg gtgaaatccc acggcccaac cgtggaagtg cctccgatac tgcgggccta
      121 gaggggaaca ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgtgctgga ttcctactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014540             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14707 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014540
VERSION     KHUT01014540.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14707"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           56 c           94 g           40 t
ORIGIN      
        1 gacagagggt gcgaacgttg ctcggaatta ctgggcgtaa agcgcgtgta ggtggcaccg
       61 cacgtcgggt gtgaaagccc ggggctcaac cccggacgtg cactcgatac gacggcgctg
      121 gagtactgaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggg cagatactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014541             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14708 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014541
VERSION     KHUT01014541.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14708"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttttt
       61 taagatggtt gtgaaatccc tgggcttaac ctaggaactg caaccatgac tggaaggcta
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014542             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14709 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014542
VERSION     KHUT01014542.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14709"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           91 g           46 t
ORIGIN      
        1 tacgtagggg gccaacgttg ctcggaatca ctgggcataa agcgcacgta ggtggctttg
       61 taagtctggc gtgaaatccc ccggcccaac cggggaactg cgtcggatac tgcaaggcta
      121 gagggctgga ggggagactg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg agtctctggt cagcttctga cactgaggtg cgaaagctag
      241 gggatcaaac ggg
//
LOCUS       KHUT01014543             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14710 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014543
VERSION     KHUT01014543.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14710"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           51 c           99 g           44 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggctcgt
       61 taagtcggtt gtgaaagccc ggggctcaac cccggagggt cgaccgatac tggcaagcta
      121 gagtacagaa gaggtagctg gaattcccgg tgtagcggtg agatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ctgatactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014544             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14711 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014544
VERSION     KHUT01014544.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14711"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           93 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggcttgt
       61 caagtccggg gtgaaatact tcagcttaac tggagaagtg ccttggatac tgacgagctc
      121 gagcgaggta ggggcagttg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg aactgctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014545             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14712 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014545
VERSION     KHUT01014545.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14712"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           86 g           54 t
ORIGIN      
        1 tacggagtgt gcaagcgtta ctcggaatca ctgggcataa agggcgtgta ggcgggcttt
       61 caagtcggtt gtgaaatccc tcagctcaac tgaggaactg catccgatac tgtttgtctt
      121 gaggacgaga ggggagagtg gaacttctgg tggagcggtg aaatgcgtag atatcagaag
      181 gaacaccggt ggcgaaggcg actctctggc tcgtatctga cgctgaaacg cgaaagcttg
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014546             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14713 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014546
VERSION     KHUT01014546.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14713"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacgaaccaa gcaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtcgat
       61 caagtctgga gtgaaatctt tcggctcaac cggaaaagtg ctttggatac tgatcggcta
      121 gaagggggta ggggttgcgg gaacagccag tggagcggtg aaatgcgttg atattggctg
      181 gaacgccggt ggcgaaagcg cgcaactgga cctctcttga cgctgaggcg cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014547             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14714 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014547
VERSION     KHUT01014547.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14714"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 tacagaggcc tcgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggcggtgacg
       61 taagtcagat gtgaaagccc tgggcttaac ctgggaactg catttgatac tgcgttgcta
      121 gagtgcgaga gaggaaaggg gaattcccgg tgtaggggtg aaatccgtag atatcgggag
      181 gaataccgat ggcgaaggca cctttctggc tcgtaactga cgctgagatt cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014548             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14715 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014548
VERSION     KHUT01014548.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14715"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           82 g           55 t
ORIGIN      
        1 tacggaggtg gcaagcgtta ctcggaatta ttgggtgtaa agggcaagta ggtggcttgg
       61 taagtgagag gtgaaatact ccggctcaat cggagaactg cctttcatac tgccttgctt
      121 gaggatgaca gggggaaagg gaattccagg tgtaagggtg aaatctgtag atatctggaa
      181 gaacaccagc ggcgaaggcg ctttcctggg tcactcctga cactaaattg cgaaagctag
      241 gggagcaaac aga
//
LOCUS       KHUT01014549             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14716 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014549
VERSION     KHUT01014549.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14716"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           86 g           55 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagcgta ggtggtagtg
       61 taagtcagat gtgaaagccc gttgcttaac agcgggattg catcagaaac tacattacta
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacatcagt ggcgaaggcg gcttactgga ctgtaactga cactaaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014550             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14717 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014550
VERSION     KHUT01014550.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14717"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggtgtaa agggcgtgca gccgggaatg
       61 caagtcagat gtgaaatcca tgggcttaac ccatgaactg catttgaaac tgtatttctt
      121 gagtactgga gaggcaatcg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gattgctgga cagcaactga cggtgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014551             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14718 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014551
VERSION     KHUT01014551.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14718"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           89 g           49 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattca ctgggcgtaa agcgcatgca ggtggcactg
       61 taagtagggc atgaaatctc ctggcttaac tgggagacgc tgttctaaac tgcagagctt
      121 gaggctgaga gagggaagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccgt ggcgaaagcg gcttcctggc tcagtcctga cactcagatg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014552             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14719 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014552
VERSION     KHUT01014552.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14719"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           96 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctcag
       61 taagtccgct ctgaaagtcc aaggctcaac cttgggatgc gggtggatac tgctgggctc
      121 gagtccggaa gaggcgggtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gcccgctggg acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014553             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14720 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014553
VERSION     KHUT01014553.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14720"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           48 c           84 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggattta ttgggcgtaa agagctcgta ggctgagtgg
       61 aaagttagat gtgaaatccc ggggctcaac cccggacccg catctaaaac tcccattcta
      121 gaggatggac ggagaaaagg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg cttttctagt ttagacctga cgctgaggag cgaaagcgag
      241 gggagcaaac agg
//
LOCUS       KHUT01014554             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14722 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014554
VERSION     KHUT01014554.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14722"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           49 c           85 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcagac gtgaaatccc ccagcttaac ctgggaactg cgtctgatac taggaggctt
      121 gagtatggga gagggatgta gaattccagg tatagcggtg aaatgcgtag atatctggag
      181 gaataccgat ggcaaaggcg gcatcctgga ccataactga cgctaaggcg cgaaagctag
      241 gagagcaaac ggg
//
LOCUS       KHUT01014555             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14723 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014555
VERSION     KHUT01014555.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14723"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           47 c           92 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggtggcttct
       61 taagtctggt ttgaaagctg gtggctcaac catcagatgt ggctggaaac tggggagctt
      121 gaatgggttg gccgtcgctg gaacgggtca tgtagcggtg aaatgcatag atatgaccca
      181 gaaccccgat tgcgaaggca ggcgacgagg acctgattga cactgaggca cgagagcatg
      241 gggagccaac agg
//
LOCUS       KHUT01014556             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14724 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014556
VERSION     KHUT01014556.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14724"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           88 g           49 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggactgg
       61 caagtgcttt gtgaaatccc acggctcaac cgtggaatcg cttggcatac tgctggtctt
      121 gagccaccta ggggcaactg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg ggtgtgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014557             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14725 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014557
VERSION     KHUT01014557.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14725"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           79 g           60 t
ORIGIN      
        1 tacgagagcc tcaagcgtta tccggaatta ttgggcgtaa agggtgcgta ggttgttttg
       61 ttagtctttt gtcaaatccc cgagcttaac ttggggtctg caatggaaac ggcaaaactt
      121 gaaagtgtga gaggcgtacg gaactcatgg tgtaggggta aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaagca gtacgctggc gcatatttga cactgaagca cgaaagcgtg
      241 ggtcgcgaat ggg
//
LOCUS       KHUT01014558             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14726 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014558
VERSION     KHUT01014558.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14726"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           95 g           46 t
ORIGIN      
        1 tacagagggg gccagcgttg ttcggaatta ctgggtgtaa agggtgcgca ggtggtccgc
       61 taagttcgat gtgaaatccc tgggctcaac tcaggaacgg cattggatac tggcgggcta
      121 gagtaccgga gagggaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gcttcctggc cgagtactga cgctcatgca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014559             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14727 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014559
VERSION     KHUT01014559.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14727"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           88 g           54 t
ORIGIN      
        1 tacgaagggt gcgagcgtta ttcggaatga ctgggtgtaa agagcgtgga ggcggtttgt
       61 taagttagtt gttaaatcgc ccggcttaac cgggcacatg cgattaaaac tgacaagcta
      121 gagtgcgaga gagaaaggta gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaataccggt tgcgaaggcg gccttttggc tcgttactga cgctggagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014560             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14728 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014560
VERSION     KHUT01014560.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14728"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           57 c           92 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggctggccgc
       61 caagtcaggg gtgaaatcct ccagcttaac tggagaactg cctctgatac tggcggcctc
      121 gaggaaggta ggggcgtgtg gaactctagg tggagcggtg aaatgcgttg atatctagag
      181 gaactccggt ggcgaaggcg acacgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014561             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14729 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014561
VERSION     KHUT01014561.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14729"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           88 g           55 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggtttaa
       61 taagttagtt gtgaaattcc tgggctcaac ctagggaggt caactaagac tgttagactt
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014562             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14730 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014562
VERSION     KHUT01014562.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14730"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           90 g           56 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggtttag
       61 taagttggaa gtgaaatccc ggagcttaac ttcggaattg ctttcaaaac tacttaacta
      121 gagtacggta gggggtgatg gaattccaag tgtagaggtg aaattcttag atatttggag
      181 gaacaccggt ggcgaaggcg gtcacctggg ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014563             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14731 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014563
VERSION     KHUT01014563.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14731"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggttgctttg
       61 taagttggat gtgaaatgtc cgggcttaac ctggaacggc catccaatac tgcaaagcta
      121 gagtacagta gagggaaggg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg ccttcctgga ctgatactga cactgaggcg cgacagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014564             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14732 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014564
VERSION     KHUT01014564.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14732"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           85 g           56 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggttata
       61 taagtccgat gtgaaatccc acggctcaac cgtggaactg cgttggatac tgtatgactt
      121 gagtaccgga gaggtaaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccaat ggcgaaggca ccttactggt cggttactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014565             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14733 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014565
VERSION     KHUT01014565.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14733"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           48 c           81 g           49 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa aggacgcgta ggcggcaaag
       61 aaagtcgggt gtgaaatccc ggagctcaac tccggaacag cactcgaaac tccttttcta
      121 gaggataggc ggggaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttattcctga cgctgaggcg tgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014566             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14734 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014566
VERSION     KHUT01014566.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14734"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           90 g           46 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcggaagca
       61 caagtctgat tttaaagacc atagctcaac tatgggaaag gattggagac tgtgctacta
      121 gacctctgga gagagaactg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccaat ggcgaaggca ggttcttgga cagaaggtga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014567             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14735 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014567
VERSION     KHUT01014567.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14735"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           81 g           55 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtttgta ggtggttcta
       61 ttagtcttct gttaaagctc tcggctcaac cgggaaagtg caggggaaac ggtagaacta
      121 gaggatgcga gaggtgtgtg gaactcatag tgtaggggta aaatccgttg atattatggg
      181 gaacaccaaa agcgaaggca gcacactggc gcactcctga cactgaaaaa cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014568             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14736 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014568
VERSION     KHUT01014568.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14736"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           59 c          106 g           40 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggaatca ctgggcgtaa agcgcgtgta ggcggtgcgg
       61 gaagtctgct gtgaaagcgc ctggcttaac tgggcgaggt cggcggaaac tctcgcactt
      121 gaggccggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag agctcgggag
      181 gaacacccgt ggcgcaggcg gccccctggg ccgcgcctga cgctgagacg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014569             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14737 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014569
VERSION     KHUT01014569.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14737"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           44 c           92 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggttatg
       61 tgtgttagat gtgaaagccc cgggcttaac ccgggaatgg catttaaaac tgcatagcta
      121 gagtgaagta gaggtgagtg gaatttccag agtagcggtg aaatgcgtag atactggaag
      181 gaacaccggt ggcgaaggcg gctcactgga cttcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014570             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14738 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014570
VERSION     KHUT01014570.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14738"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           96 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cgcgtccgct gtgaaaactc agggcttaac cctgagcctg cagtgggtac gggcagacta
      121 gagtgtggta ggggagatcg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gatctctggg ccataactga cgctgaggag cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01014571             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14739 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014571
VERSION     KHUT01014571.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14739"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacggagggt gcaaacgttg tccggaatta ctgggcgtaa agcgcatgta ggtggttttg
       61 taagtcagcg atgaaagacc gacgcttaac gtcggaaccg ttgctgatac tgcaaagctt
      121 gaggtagaca ggggtaagcg gaactttagg tgtaggagtg aaatccgtag atatctaaag
      181 gaacaccaat ggcgaaggca gcttactggg tctattctga cactgagatg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014572             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14740 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014572
VERSION     KHUT01014572.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14740"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           40 c           93 g           55 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatga ctgggcgtaa agggcgcgta ggcggtttga
       61 taagtttggt gtgaaagtcc tgggctcaac ctggggattg cactggatac tgttggacta
      121 gagatcgaga gaggaaaatg gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccggt ggcgaaggcg gttttctggc tcgatactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014573             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14741 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014573
VERSION     KHUT01014573.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14741"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcgaaatta ctgggcgtaa agggtgcgta ggtggcaact
       61 taagttactt gtgaaagtcc taggcttaac ctaggttgga cgagtgatac tgagtagcta
      121 gagtacagga gagggcagtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcggt ggcgaaggcg gctgcctggt ctgatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014574             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14742 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014574
VERSION     KHUT01014574.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14742"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           93 g           46 t
ORIGIN      
        1 tacgaactgt gcaaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggctcgt
       61 taagcagggt gtgaaagccc ccggctcaac cggggaattg cgccttgaac tagcgggctg
      121 gagtgagata ggggtgtgcg gaacttccag tggagcggtg aaatgtgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcacactggg tctcaactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014575             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14743 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014575
VERSION     KHUT01014575.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14743"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           55 c           76 g           53 t
ORIGIN      
        1 tcccagcagt ccaagtcgca accacatttg ttgggtctaa aacatccgta gcttgcttcg
       61 taagtctcct gtgaaatcct tcatcctaag tgaagaactg caagagatac tgcggagcta
      121 gagaccggga ggcgtaatga gtacgtacag ggaagcggta aaatgcgttg atcctgtatg
      181 gactaccagt cgcgaaggcg cgttacgagc acggatctga cagtgaggga tgaaggctag
      241 gggcgcaaag tgg
//
LOCUS       KHUT01014576             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14744 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014576
VERSION     KHUT01014576.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14744"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           98 g           42 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctcg
       61 caagtcccgt gtgaaatccc tcggcttaac cgaggaatgg cacgggaaac tgcggggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgatactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014577             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14745 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014577
VERSION     KHUT01014577.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14745"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           89 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta ctcggaatta ctgggcgtaa agcgcgtgta ggtggtctta
       61 taagtctggt gtgaaatccc ggggctcaac cccggaagtg cattggatac tgtaagacta
      121 gagtgcagga gaggatggcg gaattccagg tgtagaggtg aaattcgtag atatctggag
      181 gaacatcaga ggcgaaggcg gctatctgga ctgcaactga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014578             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14747 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014578
VERSION     KHUT01014578.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14747"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           88 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggtttga
       61 taagttagat gtgaaatccc tgggcttaac ctaggaactg catctaagac tgttgagctg
      121 gagtatgata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga ttaatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014579             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14748 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014579
VERSION     KHUT01014579.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14748"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           52 c           87 g           56 t
ORIGIN      
        1 tacggaaggt ccgggcgtta tccggattta ttgggtttaa agggagcgta ggccgccttt
       61 taagcgtgtt gtgaaatgca gtcgctcaac gtctgcactg cagcgcgaac tggagggctt
      121 gagtgcacgg gaagctggcg gaattcgtcg tgtagcggtg aaatgcttag atatgacgaa
      181 gaaccccgat tgcgaaggca gctggctgta gtgccactga cgctgaagct cgaaagtgcg
      241 ggtatcgaac agg
//
LOCUS       KHUT01014580             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14749 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014580
VERSION     KHUT01014580.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14749"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtgcgg
       61 taagtcactt gtgaaatctc cgggcttaac tcggagcctg caggcgaaac taccgtgctg
      121 gagtgtggga gaggtgcgtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctgt ggcgaaagcg gcgcactgga ccacaactga cgctgatgcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014581             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14750 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014581
VERSION     KHUT01014581.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14750"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           92 g           48 t
ORIGIN      
        1 tacgaaaggg gctagcgttg ttcggattta ctgggcgtaa agcgcacgta ggcggcttgg
       61 caagttgggg gtgaaatccc cgggctcaac ccgggaactg ccttcaagac tgctaggcta
      121 gaggatgcga gaggttggcg gaattccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg gccaactggc gcatttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014582             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14751 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014582
VERSION     KHUT01014582.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14751"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           97 g           48 t
ORIGIN      
        1 tacgtaggca gcaagcgttg ttcggagtta ctgggcgtaa agagtgcgta ggcggtttcg
       61 caagtttggt gtgaaatctc ccggcttaac tgggagggtg cgccgaaaac tgcggggctt
      121 gagggtggga gaggtaagcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggtgtagacg gcttactgga ccacagctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014583             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14752 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014583
VERSION     KHUT01014583.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14752"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c           96 g           50 t
ORIGIN      
        1 tacgtaggtg acgagcgtta tccggaatta ttgggcgtaa agcgcacgca ggcggtgctt
       61 caagtctgac gtgaaatctc tcggcttaac cgggagcggt cgttggaaac tgaggtgctt
      121 gaggcaagga gaggggtgtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacatcagt ggcgcaagcg gcaccctggc cttcgcctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014584             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14753 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014584
VERSION     KHUT01014584.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14753"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           54 c          106 g           38 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agggtgcgca ggcggcctag
       61 gaagtggcgt gtgaaatctc ctggctcaac tgggagggtg ggcgccaaac tggtaggctt
      121 gaggctcgga ggggggcgtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgac ggcgtaggca gcgccctgga cgagacctga cgctcaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01014585             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14754 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014585
VERSION     KHUT01014585.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14754"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           60 c          111 g           34 t
ORIGIN      
        1 gacgtagggt gcaagcgttg tccggagtta ctgggcgtaa agggcgcgta ggcggcggcc
       61 cgcgtcgcac gtgaaagccc ccggctcaac cggggagggt cgtgcgagac ggggtggctg
      121 gaggcaggga gaggctggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacacccga ggcgaaggcg gccagctggc cctgacctga cgctgaggcg cgaaggctcg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014586             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14755 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014586
VERSION     KHUT01014586.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14755"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           45 c           83 g           48 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcgggtagg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaacag catatgaaac taccgatcta
      121 gagggtaggc ggagaaaata gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggcgaaggcg attttctagc ttatacctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014587             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14756 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014587
VERSION     KHUT01014587.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14756"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           95 g           48 t
ORIGIN      
        1 tacagagggg gcaagcgtta ttcggattta ttgggcgtaa agggcgcgta ggcggcttaa
       61 caagtcaggg gtgaaatccc tcggctcaac cgaggaactg cctctgaaac tgtttggctt
      121 gagaccggga gagggtggtg gaattcccag tgtagcggtg aaatgcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctgga ccggttctga cgctgaggcg cgaaagtgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014588             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14757 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014588
VERSION     KHUT01014588.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14757"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           41 c           90 g           56 t
ORIGIN      
        1 tacgtaggga gcgagcgttg tccggaatta ctgggtgtaa agggagcgta ggcgggattg
       61 caagtcagat gtgaaataca taggcttaac ctatgggctg catttgaaac tgtagttctt
      121 gagtgaagta gaggcaagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gcttgctggg ctttaactga cgctgaggct cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014589             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14758 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014589
VERSION     KHUT01014589.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14758"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           58 c           94 g           44 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgggtgta ggcggttctt
       61 taagtcggtc gtgaaatccc acggctcaac cgtggaattg cgtccgatac tggagaactt
      121 gaggacggta gaggtcggtg gaattcccgg tgtaccggtg aaatgggcag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacc cgaaagccag
      241 ggtagcgaac cgg
//
LOCUS       KHUT01014590             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14759 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014590
VERSION     KHUT01014590.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14759"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           90 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggaatca ttgggcgtaa agcgcgtgta ggcggttccg
       61 taagtccgct ctgaaagccc aaggctcaac cttgggaggc gggtggatac tacggaactg
      121 gagtacggaa gaggagattg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaac agcgaaggca gatctctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014591             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14760 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014591
VERSION     KHUT01014591.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14760"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           94 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctcg
       61 taagtcggga gtgaaagccc tgggctcaac ccaggaagtg ctttcgaaac tgcgaggctt
      121 gagtatggaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctggg ccaatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014592             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14761 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014592
VERSION     KHUT01014592.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14761"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           94 g           48 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggttggg
       61 taagtcgggt gtgaaagccc tgggcttaac ctgggaatgg cattcgagac cacctagcta
      121 gagtctggta gagggaagtg gaatttccgg tgtagcggtg aaatgtgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg acttcctgga ccaagactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014593             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14762 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014593
VERSION     KHUT01014593.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14762"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           38 c           83 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggcaagg
       61 taagttagat gtgaaatccc tgggctcaac ctgggaattg cgtttaaaac tgcttagcta
      121 gagtattgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga cagatactga cactgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014594             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14763 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014594
VERSION     KHUT01014594.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14763"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           92 g           49 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcata ggcggttttg
       61 taagtcagac gtgaaatccc caggcttaac ttgggaactg cgtctgatac tgcagaactt
      121 gagtgcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014595             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14764 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014595
VERSION     KHUT01014595.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14764"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacggaggga gcgagcgttg ttcggaatta ctgggcgtaa agggtgcgca ggcggctttg
       61 taagttggga gtgaaatccc tgggcttaac ccaggaattg ctttcaaaac tacaaagcta
      121 gagtctcgga gaggttagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgtaagcg gctaactaga cgagtactga cgctcaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014596             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14765 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014596
VERSION     KHUT01014596.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14765"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           39 c           93 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgagta ggcggatgta
       61 taagtgaggt gataaagaca tgggcttaac ctatgggtga caccttagac tgtatatctg
      121 gagacaagga gaggggagtg gaatttccgg aggagtggtg aaatacgtag atttcggaag
      181 gaacaccgga ggcgaaggcg actctctggc cttgttctga cgctgaggag cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014597             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14766 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014597
VERSION     KHUT01014597.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14766"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           62 c           99 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 ccagtccggg gtgaaagccc acggctcaac cgtggaagcg ccttggatac tgcgagtctc
      121 gaggggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga ccccttctga cgctgatgcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014598             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14767 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014598
VERSION     KHUT01014598.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14767"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           86 g           50 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggcctga
       61 aaagtcggat gtgaaattcc ggggctcaac cccggagctg catttgaaac tttcaggcta
      121 gaggatagta agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggtgaagacg gttttctgtg ctataactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014599             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14768 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014599
VERSION     KHUT01014599.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14768"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           60 c          100 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 tgcgtccgtg gtgaaagccc ccggcttaac cggggaagtg ccgcggatac gagcaagctc
      121 gagtggggta ggggcagacg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacaccggt ggcgaaagcg gtctgctggg ccccttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014600             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14769 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014600
VERSION     KHUT01014600.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14769"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           91 g           43 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agagtgcgta ggcggcttgc
       61 caggcggggt gtgaaatccc tcggcccaac cgaggaatgg cgctccgaac cggcaggctc
      121 gaggaagata gaggcgagcg gaacagatgg tggagcggtg aaatgcgttg atatcatctg
      181 gaacacctgt ggcgaaagcg gctcgctggg tcttttctga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014601             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14770 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014601
VERSION     KHUT01014601.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14770"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           83 g           48 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggatctg
       61 aaagtcagat gtgaaatccc ggggctcaac cccggaacag catttgaaac tccagatctt
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg gttttctagc ttactcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014602             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14771 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014602
VERSION     KHUT01014602.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14771"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           81 g           58 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcgggtgtg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccatgggtac tgcatatctt
      121 gaatgttgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcactaca caggaattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014603             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14772 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014603
VERSION     KHUT01014603.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14772"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           84 g           54 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggaatta ctgggcgtaa agcgcatgta ggtggatatt
       61 taagtcgggt gtgaaagccc cgggctcaac ctgggaacag cactcgatac tggatatctc
      121 gagtatggta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacatcagt ggcgaaggcg gctacctgga tcaatactga cacttaagtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014604             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14773 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014604
VERSION     KHUT01014604.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14773"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           89 g           54 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggtgcgta ggcggtttat
       61 taagtcagat gtgaaattcc caggcttaac ctggggactg catttgaaac tggtaggctg
      121 gagtgcagga gaggaaagcg gaattcctag tgtagcggtg aaatgcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctttctgga ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014605             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14774 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014605
VERSION     KHUT01014605.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14774"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           41 c           76 g           60 t
ORIGIN      
        1 tacgtagggt gcgagcgttg ctcggaatta ctgggcgtaa agcacatgta ggctgtttta
       61 taagttagag gtgtaatccc tgggctcaac tcaggaactg cttctaaaac tgtaagacta
      121 gagtgtggta gagggtagcg gaatttctag tgtagaggta aaattcatag atattagaag
      181 gaacaccaaa agcgaaagca gctatctggg ccattactga cgctgagatg tgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014606             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14775 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014606
VERSION     KHUT01014606.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14775"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           90 g           53 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgta ggcggttaat
       61 taagtcagat gtgaaaaccc ggggcttaac tccgggattg catttgaaac tggatagctt
      121 gagtgcagga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg acttactgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014607             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14776 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014607
VERSION     KHUT01014607.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14776"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           48 c           92 g           44 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgagtgta ggcggtccta
       61 aaagtcgggt gtgaaagccc cgggctcaac ccgggaagtg cactcgaaac tgcagggcta
      121 gagtatcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaatactga cgctgagact cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014608             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14777 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014608
VERSION     KHUT01014608.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14777"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           86 g           49 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcacgta ggtggatgct
       61 taagtcgaat gtgaaacccc tgagctcaac ttggggactg catgcgatac tgaacatcta
      121 gagtatagta gagggaggtg gaatttccgg agtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gcctcctggg ctaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014609             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14778 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014609
VERSION     KHUT01014609.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14778"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           99 g           40 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtccca
       61 taagtcccgc gtgaaatccc ccggctcaac tggggaactg cgcgggagac tgcggggctt
      121 gagttcggga gagggaagcg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccggt ggcgaaggcg gcttcctgga ccgacactga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014610             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14779 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014610
VERSION     KHUT01014610.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14779"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           87 g           47 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggctgatctg
       61 caagtcaggt gtgaaatccc ggggcttaac cccggaagtg cgcctgaaac tacaagtcta
      121 gaatgtcgga gagggaaggg gaatatctca tgtaggggta aaatccgtag atatgagatg
      181 gaacaccaga ggcgaaggcg ccttcctgga cgactattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014611             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14780 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014611
VERSION     KHUT01014611.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14780"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           38 c           81 g           63 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ttgggtttaa agggtgcgta ggcggcttat
       61 taagtcagtg gtgaaatatt acagcttaac tgtgagggtg ccattgatac tgatgagctt
      121 gagtaaagcc gaggtaggcg gaattgacgg tgtagcggtg aaatgcatag atatcgtcaa
      181 gaacaccgat agtgaagaca gcttactaga cttatactga cgctgaggca cgaaagtgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014612             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14781 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014612
VERSION     KHUT01014612.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14781"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           90 g           49 t
ORIGIN      
        1 tacgtagggc gcaagcgtta tccggaatta ttgggcgtaa agagctcgta ggcggtttgt
       61 cacgtctgct gtgaaatctg ggggctcaac ccccagcctg cagtgggtac ggacagacta
      121 gagtgtggta ggggagactg gaattcctgg tgtagcggtg aaatgctcag atatcaggag
      181 gaacaccaat ggcgaaggca ggtctctggg ccactactga cgctgaggag cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014613             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14782 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014613
VERSION     KHUT01014613.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14782"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           84 g           55 t
ORIGIN      
        1 tacgaagggt cctagcgttg ttcggaatca ttgggcgtag agagcacgta ggtagtctag
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg cacttgaaac tgcatgactt
      121 gagtacggaa gaggtaagcg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gcttactggt cctgtactga cactgaggtg cgaaagcatg
      241 gggatcaaac agg
//
LOCUS       KHUT01014614             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14783 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014614
VERSION     KHUT01014614.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14783"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           52 c          100 g           40 t
ORIGIN      
        1 tacgtaggag gcaagcgtta tccggaatta ctgggcgtaa agggcgtcta ggtggcgggc
       61 caagcggggt gtgaaagctc ctggcttaac tgggagagtg cgctccgaac tggccagcta
      121 gaggacaggc gacgtggatg gaatacccgg tggagtggtg aaatgcgtag agatcgggtg
      181 gaacaccagt ggcgaaggcg gtccacgagc ctgtaactga cactgaagcg cgagagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014615             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14784 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014615
VERSION     KHUT01014615.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14784"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           92 g           48 t
ORIGIN      
        1 tacgaagggg gcaagcgttg ctcggaatca ctgggcgtaa agggcgcgta ggcggatcgt
       61 taagtcgggg gtgaaatcct ggggctcaac ctcagaattg ccttcgatac tggcgatctc
      121 gagaccggaa gaggtaagtg gaattgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gcttactggt ccggttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014616             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14785 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014616
VERSION     KHUT01014616.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14785"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           85 g           56 t
ORIGIN      
        1 gacagagggt gcaagcgtta atcgaaataa ctgggcgtaa agagtacgta ggcggaagaa
       61 taagtcatgt gtgaaatatc ggggctcaac ctcgggatgt catgtgatac tgtttttcta
      121 gagtttggta gaggtaagtg gaatttccgg tgtagcggtg aaatgcttag atatcggaag
      181 gaacaccagt ggcgaaggcg gcttactggg ccaatactga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014617             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14786 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014617
VERSION     KHUT01014617.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14786"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           90 g           53 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctctg
       61 taagtcagga gtgaaagccc caggcttaac ctgggaagtg cttttgatac tgcagagctt
      121 gaatgtggga gaggatggtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaactccggt ggcgaaggcg gccatctggc ccaacattga cactgagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014618             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14787 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014618
VERSION     KHUT01014618.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14787"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           97 g           44 t
ORIGIN      
        1 tacagagggg gcaagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtgcga
       61 tcagtcgaat gtgaaagccc ccggctcaac tggggagggt cattcgatac tgtcggactc
      121 gaaggcagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcttcctggc ctgttcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014619             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14788 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014619
VERSION     KHUT01014619.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14788"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           89 g           49 t
ORIGIN      
        1 tacagaggcc acaagcgtta atcggaatca ctgggcttaa agggtgcgta ggcggcgccg
       61 caagtgcttt gtgaaatccc atcgcttaac gatggaattg ctcggcatac tgcggtgctt
      121 gaggtcggta gaggtcgtcg gaacagtagg tggagcggtg aaatgcgtag atatctactg
      181 gaacgccaaa ggtgaagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014620             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14789 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014620
VERSION     KHUT01014620.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14789"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctggg gtgaaatcct tgagcttaac tcaagaagtg ccctagaaac ggtgagactt
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagc ggcgaaggcg gcgacctgga cagtaactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014621             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14790 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014621
VERSION     KHUT01014621.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14790"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccag
       61 caagcgggga gtgaaagccc tcggcttaac cgaggaagtg ctttccgaac tactggactt
      121 gaatactgaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg cagatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014622             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14791 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014622
VERSION     KHUT01014622.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14791"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           89 g           49 t
ORIGIN      
        1 tacgagggtg tcaaacgttg tccggaatta ttgggcgtaa agggcgcgta ggcggccaag
       61 taagtcagat gtgaaatcgc tgggctcaac ccagcaattg catttgaaac tgctaggcta
      121 gagatcggta gaggagggcg gaattcctgg tgtagcggtg aaatgcgttg atatcaggag
      181 gaacacccgt ggcgaaggcg gctctctgga ccgattctga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014623             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14792 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014623
VERSION     KHUT01014623.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14792"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           91 g           47 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgcatgca ggcggattgt
       61 taagtcgagg gtgaaatccc agggctcaac cctggaactg ccttcgatac tggcaatctt
      121 gaggccggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccggtactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014624             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14793 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014624
VERSION     KHUT01014624.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14793"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           93 g           47 t
ORIGIN      
        1 tacgggggga gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggtcagg
       61 taagtgagat gtgaaatcct ccggctcacc cggagaaatg catctcatac tgcttgacta
      121 gagtgcggaa gaggaatcgg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg cgattctggt ccgttactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014625             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14794 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014625
VERSION     KHUT01014625.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14794"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           41 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcgcgta ggtggttata
       61 taagtgagtt gtgaaatccc tgggcttaac ctaggaactg caactgagac tgtatgactg
      121 gagtatgata gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga tcaatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014626             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14796 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014626
VERSION     KHUT01014626.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14796"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           76 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattgg
       61 taagtccgtg gtgaaatctc cgagcttaac tcggaaactg ccatggatac tatcagtctt
      121 gaatatcttg gaggtaagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gcttactaca cgatgattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014627             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14797 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014627
VERSION     KHUT01014627.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14797"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           82 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agcgtatgta ggctgtacgg
       61 taagtcagat gtgaaagccc agggctcaac cctggaactg catccgatac tgccatactt
      121 gagtagtgga ggggtatatg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaacactggc ggcgaaggcg atatactgga cactcactga cgctgagata cgaaggctag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01014628             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14798 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014628
VERSION     KHUT01014628.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14798"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           89 g           46 t
ORIGIN      
        1 gacgaaccgg acaaacgtta ttcggaatta ttgggcttaa agggtgcgta ggcggccatg
       61 aaagttgggt gtgaaagccc tcggctcaac cgaggaatag cgctcaaaac tacatggctt
      121 gagtgaggca gaggtgagcg gaacttgcgg tggagcggtg aaatgcgttg atatcgcaag
      181 gaacaccagt ggcgaaggcg gctcactggg cctttactga cgctgaggca cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014629             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14799 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014629
VERSION     KHUT01014629.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14799"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           96 g           41 t
ORIGIN      
        1 aacgtaggac gcaagcgtta tccggagtta ctgggcgtaa agcgcgggca ggcggtctgt
       61 taagtctgat gtgaaatctc ctcgctcaac ggggagcagc cattggaaac tgggagactg
      121 gagtctgaca gaggtaggcg gaattccggg tggagtggtg aaatgcgtag agatccggag
      181 gaacaccaga ggcgaaggcg gcctactggg tcagaactga cgctgagccg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014630             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14800 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014630
VERSION     KHUT01014630.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14800"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggctggg
       61 taagtttgat gtgaaagctc ggagctcaac tccgaaaagg cattgaatac tatctggctt
      121 gaggatcgga ggggagactg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtctctgga cgagtcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014631             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14801 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014631
VERSION     KHUT01014631.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14801"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           57 c           96 g           41 t
ORIGIN      
        1 gacggagggt gcaaacgttg ttcggaatca ctgggcataa agcgcacgta ggccgtccgc
       61 taagttcgac gtgaaagccc ccggctcaac cggggaactg cgtcgaagac tggcggactc
      121 gaggacggtt ggggtgagtg gaactcctgg tggagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaagcg gctcactgga ccgtttctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014632             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14802 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014632
VERSION     KHUT01014632.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14802"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           85 g           57 t
ORIGIN      
        1 gacgtaggat gcaagcgttg tccggattta ttgggcgtaa agagttcgta ggcggttact
       61 taagtttggt gttaaatccc atggctcaac catggacctg catcgaatac tgggtaactt
      121 gagtgtggta gaggcgagtg gaattcccag tgtagcggtg aaatgcatag atattgggaa
      181 gaacaccggt ggcgtaggcg actcgctggg ccataactga cgctgaggaa cgaaagccag
      241 gggagcaaat ggg
//
LOCUS       KHUT01014633             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14803 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014633
VERSION     KHUT01014633.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14803"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           97 g           43 t
ORIGIN      
        1 tacggagggt gcgagcgttg ttcggaatta ttgggcgtaa agggcgtgca ggcggcttgt
       61 caggtccgat gtgaaagccc cgggctcaac ccgggaagtg cattggaaac cggcaagctt
      121 gaatacggga gagggcagtg gaattcctgg tgtaggggtg aaatccgcag atatcaggag
      181 gaacaccggt ggcgaaggca gctgcctgga ccgatattga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014634             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14804 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014634
VERSION     KHUT01014634.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14804"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           84 g           53 t
ORIGIN      
        1 tacagaggtt gcgagcgttg ttcggattta ctgggcgtaa agggtgcgta ggcggtctta
       61 tgtgtcagat gtgaaatccc atggctcaac catggaactg catttgaaac tgtaggacta
      121 gagtatcgga gaggtgagtg gaattcttgg tgtagcggtg aaatgcgtag atatcaagaa
      181 gaacaccagc ggcgaaagcg actcactgga cgaatactga cgctgaggca cgaaagccag
      241 gggagcaaag agg
//
LOCUS       KHUT01014635             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14805 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014635
VERSION     KHUT01014635.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14805"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           87 g           51 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcaata
       61 caagtcaggg gtgaaatccc agagcttaac tctggaactg cctttgaaac tgtattgctt
      121 gagtatcgga ggggagtgcg gaattgctaa tgtagcggtg aaatgcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gcactctgga cgaacactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014636             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14806 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014636
VERSION     KHUT01014636.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14806"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatga ctaggcgtaa agggcaggta ggcggtttgg
       61 taagtctgtt gtgaaagctc ccggcttaac tgggagaggc caacagatac tgctaggctt
      121 gagtgtggga gaggttactg gaattcccgg tgtagcggtg aaatgcgcag agatcgggag
      181 gaacaccaat ggcgaaagca agtaactgga ccattactga cgctcagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014637             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14807 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014637
VERSION     KHUT01014637.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14807"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           57 c           89 g           42 t
ORIGIN      
        1 tacagagggc ccgagcgtta atcggaatca ctgggcttaa agcgtacgca ggcggccctg
       61 aaggcatcgt gtgaaatccc ccggcttaac cggggaactg cacggtgaac cacagggctt
      121 gaggcaggta ggggtcagtg gaaccatgag tggagcggtg aaatgcgtag atattcatgg
      181 gaacgccaat ggcgaaggca gctgactggg cctgatctga cgctcatgta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014638             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14808 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014638
VERSION     KHUT01014638.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14808"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           52 c           93 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cgcccgaaac tgcatagctt
      121 gagtttcgga gagggtggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccacctggc cgaaaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014639             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14809 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014639
VERSION     KHUT01014639.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14809"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           51 c          105 g           42 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggaatta ctgggcgtaa agagcgtgta ggcggtctgc
       61 caagtctgtt gtgaaagcgc ctggcttaac tgggcgaggt cgacggaaac tggcaaactg
      121 gaggccggta gaggagggtg gaattcccgg tgtagtggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gccctctggg ccgggcctga cgctgagacg cgagggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014640             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14810 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014640
VERSION     KHUT01014640.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14810"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           83 g           53 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaataa ttgggcgtaa agggcgcgta gatggtatgt
       61 taagtttgtt gttaaatcct tcggctcaac tgaagatcag caacgaaaac tggcgtgcta
      121 gagggtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccacacctga cattgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014641             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14811 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014641
VERSION     KHUT01014641.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14811"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           90 g           51 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgtgtgta ggtggttgtc
       61 gtagtcgggt gtgaaagcct tgagctcaac tcaagaaatg cactcgatac tcggcggcta
      121 gaggaccgga gaggatagtg gaattcccag tgtagtggtg aaatacgtag agattgggaa
      181 gaacaccagt ggcgaaggcg gctatctgga cggtttctga cactaagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014642             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14812 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014642
VERSION     KHUT01014642.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14812"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           44 c           95 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttaat
       61 taagtgagat gtgaaatccc cgggcttaac ctgggaactg catcttagac tagttgactg
      121 gagtgcagta gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg accacctgga ctgagactga cgctgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014643             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14813 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014643
VERSION     KHUT01014643.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14813"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           43 c           83 g           50 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggatata
       61 caagttagat gtgaaagccc ggggctcaac tccggaacgg catttaaaac tgtaaatcta
      121 gagggtaggc ggagaaaatg gaattccaca tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggcgaaggcg attttctagt ctatacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014644             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14814 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014644
VERSION     KHUT01014644.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14814"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           80 g           56 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ttgggcgtaa agcgtgcgta ggcggtctag
       61 taagtcgaat gtgaaagccc agagcttaac tttggaattg cattcgaaac tactaaacta
      121 gagtatggcg ggggatagtg gaattcttag tgtaggggtg aaatccgtag atattaagag
      181 gaacaccagt ggcgaaagcg gctatctaca ccattactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014645             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14815 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014645
VERSION     KHUT01014645.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14815"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           85 g           52 t
ORIGIN      
        1 tacgaaggtg gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttg
       61 caagtcaggg gtgaaatcct aaggctcaac cttagaattg cctttgaaac tgcaaggctg
      121 gagtactgga ggggttagcg gaattgctaa tgtagaggtg aaattcgtag atattagcag
      181 gaacaccgat ggcgaaggca gctaactgga cagtcactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014646             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14816 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014646
VERSION     KHUT01014646.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14816"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           47 c           82 g           56 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggttttg
       61 ttagtcttct gttaaattct tcggcttaac cgggggtctg caaaagaaac ggcaaaacta
      121 gaggatgcga gaggtgtgcg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca gcacactggc gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014647             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14817 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014647
VERSION     KHUT01014647.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14817"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggctcaa
       61 taagtctttt gtgaaagctc ccggcttaac tgggagaggt caagagaaac tattgggcta
      121 gagtgtggta gaggaaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggtttctggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014648             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14818 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014648
VERSION     KHUT01014648.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14818"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           85 g           52 t
ORIGIN      
        1 gacaggggat gcaagcgtta ctcggaatta ctgggcgtaa agcgtctgta ggcgactttt
       61 caagtccggg gtgaaagtgc ggagctcaac tccgtgattg ccttggaaac tgtaaagatt
      121 gagctattca gaggtatctg gaatgccata agtaggggta aaatccgtag atctatggta
      181 gaacgccaaa agcgaaggca ggatactggg aatatgctga cgctgagaga cgaaagcgtg
      241 gggagcgaag ggg
//
LOCUS       KHUT01014649             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14819 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014649
VERSION     KHUT01014649.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14819"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           56 c          105 g           42 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggcgcgta ggcggtctgc
       61 tgcgtcggtg ctgaaagggt ctcgcttaac gagatcaggt gtgccgatac gagcaggctg
      121 gaggcagaga gaggttggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacgcccgt ggcgcaagcg gccaactggc tctgttctga cgctgaggcg cgacagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014650             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14820 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014650
VERSION     KHUT01014650.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14820"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          102 g           37 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctcg
       61 cgagtcaggg gtgaaagccc gcggcttaac cgcggaagcg cctttgatac tgcgggcctc
      121 gaggggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014651             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14821 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014651
VERSION     KHUT01014651.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14821"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           44 c           87 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaataa ttgggcgtaa agggtgcgca ggcggtgaat
       61 taagacagat gtgaaagccc tgggcttaac ctgggaatgg catttgtgac tgattcacta
      121 gaatatggca gagggaggtg gaattccaca tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccaat ggcgaaggca gcctcctggg ccaatattga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014652             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14822 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014652
VERSION     KHUT01014652.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14822"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           93 g           52 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggcggtctct
       61 taagtctggt gtgaaatcct ggggcttaac cccagaagtg cactggatac tgggagacta
      121 gagggcgaga gaggagaatg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcaga ggcgaaggcg gttctctggc tcgtttctga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014653             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14823 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014653
VERSION     KHUT01014653.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14823"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgtgta ggcggcctgt
       61 taagtcgact gtgaaagccc ttggctcaac caaggaagtg cagtcgaaac tggcagactc
      121 gagtcccgaa gaggatggcg gaatggccag tgtagaggtg aaattcgtag atattggttg
      181 gaacaccagt ggcgaaggcg gccatctggg aggcgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014654             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14824 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014654
VERSION     KHUT01014654.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14824"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           59 c          108 g           39 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattca ttgggcgtaa agcgcgcgca ggcggccggc
       61 agagcccggc gtgaaagctc ccagcttaac tgggagaggt cgtcggggac ctggtggcta
      121 gaggccggta ggggtgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggt ggcgaaggcg gctcactggg ccggtcctga cgctcaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014655             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14825 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014655
VERSION     KHUT01014655.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14825"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           45 c           90 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggctttc
       61 taagtctggt gtgaaagccc ggggctcaac cccggaagtg cactggatac tggaaagcta
      121 gagggcgaga gaggagattg gaattcatgg tgtaggggtg aaatccgtag atatcatgag
      181 gaacatctgt ggcgaaggcg aatctctggc tcgtttctga cactgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014656             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14826 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014656
VERSION     KHUT01014656.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14826"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           90 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agggtgcgta ggcggatctt
       61 taagttagag gtgaaagcct ggagctcaac tccagaacgg cctttaatac tggggatctt
      121 gagttcggga gaggtgagtg gaactgcgag tgtagaggtg aaattcgtag atattcgcaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014657             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14827 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014657
VERSION     KHUT01014657.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14827"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           42 c           79 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggaatta ctgggcgtaa agggtatgta ggcggaagtt
       61 taagtagaaa gtgaaaggtg agagctcaac tctgacattg cttcctatac tggacatctt
      121 gagtttcgga gaggaagatg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat agcgaaggca atcttctgga cgaatactga cgctgagata cgaaagccaa
      241 ggtagcgaac agg
//
LOCUS       KHUT01014658             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14828 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014658
VERSION     KHUT01014658.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14828"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           35 c           69 g           68 t
ORIGIN      
        1 tacgaagggg gtgagcgtta tccgaaatga ttgggcgtaa agcgttcgta ggttgtagat
       61 tctgtctttc gttaaagctt gattaaaaag tcaaaccagc gttagatact tttctacttg
      121 agtatattag aggacaatag aatttcataa ggaatggtag aatatctaaa tctatgaagg
      181 aatgccggtg gcgaaagcga ttgtctggaa tctatactga cactgaggaa cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01014659             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14829 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014659
VERSION     KHUT01014659.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14829"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           44 a           63 c          110 g           36 t
ORIGIN      
        1 tacgtagggt ccgagcgttg tccggagtta ctgggcgtaa agcgcgcgca ggcggcggtg
       61 ttggcccggc gtgaaagccc ccggctcaac cggggagagt cgtcggggac cgcaccgctt
      121 gagggcggta ggggccggtg gaatgcctgg tgtagtggtg aaatgcgtag agatcaggca
      181 aaacacctgg ggcgaaggcg gccggctggg ccgtccctga cgctgaggcg cgaaggcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014660             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14830 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014660
VERSION     KHUT01014660.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14830"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           95 g           45 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggttgac
       61 caagtcgagt gtgaaatacc agagcttaac tctggcaggt cgctcgatac tggtcgacta
      121 gagtgtagaa gaggatgtcg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg ggcatctggg ctaacactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014661             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14831 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014661
VERSION     KHUT01014661.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14831"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           86 g           54 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggattta ctgggcgtaa agggcgcgta ggcggtttag
       61 taagttggat gtgaaagccc tgggctcaac ccgggaattg cattcaaaac tactagactt
      121 gaattcggta gaggttggta gaattcccag tgtagaggtg aaattcgtag agattgggaa
      181 gaatacccgt ggcgaaggcg gccaactgga ccgacattga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014662             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14832 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014662
VERSION     KHUT01014662.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14832"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           89 g           51 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 aaagtcaggg gtgaaatcct ccagcttaac tggagaactg cctttgatac tgatggtctc
      121 gagtaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgtgttg atatcatcag
      181 gaactccggt ggcgaaagcg gcatgctgga ccttcactga cgctgatgcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014663             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14833 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014663
VERSION     KHUT01014663.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14833"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c          101 g           44 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggattta ctgggcgtaa agggcgcgca ggcggcgatc
       61 tttgtcaggc gtgaaagccc cgggctcaac ctgggaatgg cgcttgggac ggggtcgcta
      121 gagatcggga gaggagagtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014664             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14834 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014664
VERSION     KHUT01014664.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14834"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           84 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgtgta ggcggcgtga
       61 aaagtcagat gtgaaatctg ggggctcaac cttcaaatag catccgatac tgtcatgctg
      121 gaggtctgta gaggagtctg gaattctcgg tgtagcagtg aaatgcgtag atatcgagag
      181 gaacacttgt tgcgaaagcg agactctggg cagaaactga cgctgaaaca cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014665             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14835 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014665
VERSION     KHUT01014665.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14835"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           81 a           34 c           70 g           68 t
ORIGIN      
        1 tacggaggat acaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggttttt
       61 taagtcagta gtgaaatctt atagcttaac tataaaattg ctattgatac tggaaagcta
      121 gagtaatatt ggagtaactg gaatgtgtgg tgtagcggtg gaatgcatag atatcacaca
      181 gaacaccaat cgcgaaggca ggttattaaa tatttactga cgctgaggca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014666             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14836 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014666
VERSION     KHUT01014666.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14836"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           50 a           57 c          111 g           35 t
ORIGIN      
        1 gacagagggg gcaagcgttg tccggattga ctgggcgtac agggcgcgta ggcgggacgc
       61 gacgtgctgg gtgacaggcg acggctcaac cgtcgttgga ctcggtagac gcgcgacctg
      121 gaggccgaga gagggatgtg gaatacccgg tggagcggtg aaatgcgtag agatcgggtg
      181 gaacaccagg ggcgtaggcg gcatcctggc tcggacctga cgctgaggcg cgagagcgtg
      241 gggagcgaac tgg
//
LOCUS       KHUT01014667             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14837 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014667
VERSION     KHUT01014667.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14837"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           84 g           55 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgtatgta ggcggatgct
       61 taagtaggaa gtgaaaggtt agagctcaac tctaacactg ctccctatac tgggcgtctt
      121 gagggtcgga gaggaaagcg gaacgacacg tgtagcggtg aaatgcgttg atatgtgtcg
      181 gaacaccaat ggcgaaggca gctttctgga cgatttctga cgctgagata cgaaagccga
      241 ggtagcgaac agg
//
LOCUS       KHUT01014668             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14838 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014668
VERSION     KHUT01014668.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14838"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           65 c          105 g           32 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccgg
       61 ccagtcaggg gtgaaagccc gcggcttaac cgcggaagcg cctctgatac tgccggcctg
      121 gaggggggta ggggcgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcgctgga ccccgcctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014669             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14839 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014669
VERSION     KHUT01014669.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14839"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           95 g           47 t
ORIGIN      
        1 tacagaggtc tcgagcgtta atcggagtca ctgggcttaa agggtgcgta ggcggcgctg
       61 caagtgcttt gtgaaatccc acggctcaac cgtggaattg ctcggcagac tgcagcgctt
      121 gaggtcggta ggggtcgctg gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgaa ggtgaagaca ggcgactggg ccgattctga cgctgaggca cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014670             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14840 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014670
VERSION     KHUT01014670.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14840"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           54 c          104 g           42 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgcgca ggtggcagca
       61 caagtatggg gtgaaatctc cctgctcaac ggggagggtg ccttgtagac tgtgcagcta
      121 gagggcggta gaggggtgtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaacacccgt ggcgaaggcg gcaccctggg ccgcacctga cgctgaggcg cgaaggctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014671             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14841 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014671
VERSION     KHUT01014671.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14841"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           50 c           90 g           45 t
ORIGIN      
        1 aacgtaggaa gcgagcgtta tccggattta ctgggcgtaa agcgcatgca ggcggttcca
       61 caagttagac ttgaaagctc ccggcttaac tgggagaggc ggtttaagac tgtgggacta
      121 gaggatggac gaggagagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg gctctctaga ccgtacctga cgctcagatg cgaaagccag
      241 gggagagaac ggg
//
LOCUS       KHUT01014672             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14842 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014672
VERSION     KHUT01014672.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14842"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           48 c           86 g           54 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta ggtggcgaag
       61 caagtctaac gtgaaatctc ggggcttaac cccgaaattg cgtcggatac tgctttgctc
      121 gaggattgta gaggagagtg gaattcacgg tgtagcagtg aaatgcgtag atatcgtgag
      181 gaagaccagt tgcgaaggcg actctctggg caattcctga cactgaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014673             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14843 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014673
VERSION     KHUT01014673.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14843"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           43 c           75 g           60 t
ORIGIN      
        1 tacggaggat ccaagcgtta tccggattta ttgggtttaa agggtgcgta ggcggccgta
       61 taagtcaggg gtgaaaactt gcggctcaac cgtaagcttg cctttgatac tgtatggctt
      121 gaatatagat gaggtaggcg gaatgtgaca agtagcggtg aaatgcatag atatgtcaca
      181 gaacaccaat tgcgaaggca gcttactaaa ctaatattga cgctgatgca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014674             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14844 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014674
VERSION     KHUT01014674.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14844"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           82 g           55 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgcgta gacggcatat
       61 taagtctgta gttaaatccc tcggcttaac cgaggatatg ctgcggaaac tgataagcta
      121 gagggtgaaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt tcattcctga cgttcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014675             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14845 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014675
VERSION     KHUT01014675.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14845"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           97 g           50 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatga ctgggcgtaa agggtgcgta ggcggttggc
       61 taagttatct gtgaaagccc tgggcttaac ctgggaaggt cagataagac tggttgactt
      121 gagtatggga gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctgtctggc ctaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014676             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14846 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014676
VERSION     KHUT01014676.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14846"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           50 c           99 g           41 t
ORIGIN      
        1 aacgtaggag acgagcgtta tccggaatta ctgggcgtaa agcgcgagta ggcggctttt
       61 taagtggtct gtgaaagcgt ccggctcaac tggacgaggc cggatcagac tggaaagctg
      121 gagggtcaga gaggcaggcg gaattccggg tgtagtggtg aaatgcgtag agatccggag
      181 gaacaccagt ggcgaaggcg gcctgctggc tgacaactga cgctgaggcg cgaaagcatg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014677             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14847 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014677
VERSION     KHUT01014677.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14847"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           87 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggcgata
       61 taagtcagag gtgaaagccc agggctcaac cctggaattg cctttgaaac tatatcgcta
      121 gaatctcgga ggggttggcg gaattcctaa tgtagcagtg aaatgcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gccaactgga cgagtattga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014678             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14848 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014678
VERSION     KHUT01014678.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14848"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           93 g           40 t
ORIGIN      
        1 gacgaaccgt ccgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggatccg
       61 caagtcaggg gtgaaatccc acggctcaac cgtggaagag cctttgatac tgcgggtcta
      121 gagggatgta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcacgctgga catctactga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014679             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14849 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014679
VERSION     KHUT01014679.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14849"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           53 c           98 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ctgggcgtaa agcgcgcgta ggtgggggtg
       61 caagtccggt gggaaagccc acggctcaac cgtggaaggt cgctggaaac tgcacttctt
      121 gaggtagaca gaggagagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacccat ggcgaaggca gctctctggg tttcacctga cactgaggcg cgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014680             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14850 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014680
VERSION     KHUT01014680.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14850"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           57 c          107 g           35 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggagtta ctgggcgtaa agggtgcgca ggcggcccgg
       61 caggtgggtg gtgaaagctc ctggctcaac tgggagaggg ccatgcagac ggtcgggctt
      121 gagggtcgca gaggggcgtg gaattccggg tggagcggtg aaatgcgtag agatccggag
      181 gaacaccgac ggcgaaggca gcgccctgga cgatccctga cgctcaggca cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01014681             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14851 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014681
VERSION     KHUT01014681.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14851"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           42 c           96 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggcggtgaga
       61 tgtgtatggt gtgaaagccc cgggcttaac ctgggaagtg cattgtagac tatctgactg
      121 gagtatatga gagggtggtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcaat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014682             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14852 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014682
VERSION     KHUT01014682.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14852"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           43 c           98 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggtgtaa agggcgcgta ggcggttttg
       61 tgagtgtgat gtgaaagccc cgggcttaac ctgggaaggg catcgcaaac gataagactg
      121 gagtatatga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ataatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014683             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14853 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014683
VERSION     KHUT01014683.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14853"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           48 c           83 g           52 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggattta ctgggcgtaa agggtccgta ggcggcttag
       61 aaagttgaat gtgaaatccc gcagctcaac tgcggaactg cattcaaaac ttctaggcta
      121 gagtatgaga ggggttaagg gaattcccgg tgtaagggtg aaatctgcag agatcgggag
      181 gaacaccagt cgcgaaggcg cttaactggc tcattactga cgctcaggga cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014684             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14854 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014684
VERSION     KHUT01014684.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14854"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           85 g           55 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtaa agggcgtgta ggcggattcg
       61 taagtcagat gtgaaattcc ggggctcaac ctcggagctg catttgaaac tgtgagtctt
      121 gagggttgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggcgaaagcg gttttctagt taatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014685             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14855 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014685
VERSION     KHUT01014685.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14855"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           91 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtggca
       61 taagttaact gtgaaatccc cgggctcaac ctgggaaggt cagttaagac tgtgtgacta
      121 gagtatggga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014686             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14856 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014686
VERSION     KHUT01014686.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14856"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           42 c           95 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatca ttgggcgtaa agggcgcgta ggtggtttcc
       61 taagtctggt gtgaaagccc tggattcaat ccaggaagtg cattggatac tgggagactg
      121 gagtgcagga ggggagagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacatcgga ggcgaaggcg gctctctgga ctgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014687             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14857 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014687
VERSION     KHUT01014687.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14857"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           91 g           48 t
ORIGIN      
        1 gacggggggg gggcaagtgt tcttcggaat gactgggcgt aaagggcacg taggcggtga
       61 atcgggttga aagtgaaagt cgccaaaaag tggcggaatg ctctcgaaac caattcactt
      121 gagtgagata gaggagagtg gaatttcgtg tataggggtg aaatccgtag atctatgaag
      181 gaacgccaaa agcgaaggca gctctctggg tccctaccga tgctagggtg cgaaagaatg
      241 gggagcgaac atg
//
LOCUS       KHUT01014688             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14858 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014688
VERSION     KHUT01014688.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14858"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           57 c           98 g           41 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcaag
       61 caagtccggg gtgaaagcgt tcggctcaac cggaccaggg ccttggaaac tgcttgtctg
      121 gagggaggta ggggtccgcg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg gcggactgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014689             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14859 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014689
VERSION     KHUT01014689.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14859"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           47 c           80 g           51 t
ORIGIN      
        1 gacaggggat acaagcgtta ctcggaatca ctgggcgtaa agcgtctgta ggcttttcag
       61 aaagtctgag gtcaaatgtc ggggctcaac cccgtcaacg tcttggaaac tactgagatt
      121 gagcaatgga gaggtacctg gaatgccatg tgtaggagta aaatccgtag atatatggta
      181 gaacgcctaa agtgaagaca gggtactagc cattcgctga cgctgagaga cgaaagcgtg
      241 gggagcaaag ggg
//
LOCUS       KHUT01014690             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14860 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014690
VERSION     KHUT01014690.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14860"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           46 c           93 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggttaat
       61 taagtcagat gtgaaaggct acggctcaac cgtagagttg catttgaaac tggttaactt
      121 gagtgcagga gaggtaagtg gaattcccgg tgtagcggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gcttactggc ctgcaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014691             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14861 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014691
VERSION     KHUT01014691.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14861"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           86 g           54 t
ORIGIN      
        1 gacgagggat ccaagcgttg ttcggaatca ttgggcgtaa agcgggtgta ggtggctttg
       61 taagtcaggt gtgaaatccc agggctcaac cctggacgtg catttgatac tgcagagctt
      121 gagtgtggga gaggttacta gaatacctgg tgtagtggtg aaatacgtag atatcaggtg
      181 gaataccgga ggcgaaggcg ggtaactggc ctaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014692             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14862 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014692
VERSION     KHUT01014692.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14862"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           95 g           47 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggccttc
       61 taagtcagac gtgaaatccc caggcttaac ttgggaactg cgtctgatac tgggaggctt
      121 gaatgcggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccggt ggcgaaggcg gcatcctgga ccgacattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014693             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14863 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014693
VERSION     KHUT01014693.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14863"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           91 g           50 t
ORIGIN      
        1 tacagaggtg gcaagcgttg ttcggaatta ctgggcgtaa agggctcgta ggtggccaat
       61 taagtcaggc gtgaaatccc caggctcaac ttgggaactg cgtctgatac tggttggctt
      121 gaattcggga gagggatgca gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaataccggt ggcgaaggcg gcatcctgga ccgagattga cactgaggag cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014694             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14864 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014694
VERSION     KHUT01014694.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14864"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           90 g           52 t
ORIGIN      
        1 tacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgta ggcggttttt
       61 caagtcaggt gtgaaatctt tcggctcaac cggaaaatgg cgcttgaaac tgagaaactg
      121 gagtgtgcta ggggtgtgtg gaactgccag tggagcggtg aaatgtgttg agattggcag
      181 gaacgccggt ggcgaaagcg acacactggg gcataactga cgctgaggca cgaaagccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014695             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14865 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014695
VERSION     KHUT01014695.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14865"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           96 g           44 t
ORIGIN      
        1 cacgtaggtg gcaagcgtta tccggaatta ctgggcgtaa agggtccgca ggtgggacgg
       61 tcagtctgtg gtgacagccc ctggcttaac tgggggaggc ccatggatac tgccgttctt
      121 gagtcacgga gagggaagcg gaattccggg tgtagtggtg gaatgcgtag agatccggag
      181 gaactccaaa ggcgcaagca gcttcctggc cgaggactga cactgaggga cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014696             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14866 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014696
VERSION     KHUT01014696.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14866"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           43 c           92 g           51 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcgctg
       61 tgtgtcgaat gtgaaatacc tgggcttaac cgaggaagtg cattcgaaac tacagtgcta
      121 gaatcccaaa gaggttggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacatcggt ggcgaaggcg accaactggt tggtgattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014697             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14867 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014697
VERSION     KHUT01014697.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14867"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           46 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgat
       61 caagtcaggg gtgaaatccc gcagctcaac tgcggaagtg cctttgatac tggttgtctc
      121 gagggagata ggggcatgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccgat ggcgaaagca gcgtgctgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014698             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14868 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014698
VERSION     KHUT01014698.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14868"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           50 c           98 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agagctcgta ggcggtttgg
       61 caagtcggat gtgaaaactt caggctcaac ctggagatgc cattcgagac tgctatgact
      121 agagtccggt aggggagtgt ggaattcccg gtgtagcggt gaaatgcgca gatatcggga
      181 ggaacaccgg tggcgaaggc ggcactctgg gccggtactg acgctgagga gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01014699             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14869 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014699
VERSION     KHUT01014699.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14869"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           44 c           92 g           47 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtatta
       61 aacgtggtta tgtgaaagcc ccaggcttaa cctggggagt gcataccaaa cgatagtact
      121 ggaatacagg agagggtagc ggaatttccg gtgtagcggt gaaatgcgta gagatcggaa
      181 ggaacgtcaa tggcgaaggc agctacctgg actgatattg acgctgaggc gcgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01014700             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14870 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014700
VERSION     KHUT01014700.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14870"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           94 g           45 t
ORIGIN      
        1 tacagagggg gtaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggttccg
       61 tgcgtcagcg ctgaaatcta taggcttaac ctataggcgc gtgctgatac gacgggactt
      121 gaggctctga gagggtggtg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg accacctggc agagacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014701             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14871 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014701
VERSION     KHUT01014701.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14871"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           48 c           95 g           50 t
ORIGIN      
        1 gacgtagggg gcgagcgtta ttcggaatca ttgggcgtaa agcgctcgta ggcgggaagg
       61 caagtccgtg ttgaaagacc tcggctcaac tgagggaccg gctcggatac tgcttttctt
      121 gaggcaatca gagggtaatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gttacctggg gttgttctga cgctgaggag cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014702             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14872 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014702
VERSION     KHUT01014702.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14872"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c           98 g           43 t
ORIGIN      
        1 tacagagggg gcaagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcgtgc
       61 taagtcgaac gtgaaatccc cgggctcaac ccgggaactg cgtccgagac tggcatgctt
      121 gaatccggga gagggatgtg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacatcggt ggcgaaggcg gcatcctgga ccggtattga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014703             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14873 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014703
VERSION     KHUT01014703.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14873"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           52 c           92 g           43 t
ORIGIN      
        1 tacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggcacgt
       61 taagtcgacc gtgaaagccc atggctcaac catggaagtg cagtcgaaac tgacgagctc
      121 gagtcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctgga cgggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014704             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14874 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014704
VERSION     KHUT01014704.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14874"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           86 g           53 t
ORIGIN      
        1 tacgaaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta ggcggtttga
       61 taagtctttt gtgaaagctc ccggcttaac tgggagaggt caagagaaac tgtcaggcta
      121 gagtgtggta gaggtaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaatacctat ggcgaaagca ggttactggg ccatcactga cgctgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014705             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14875 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014705
VERSION     KHUT01014705.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14875"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           50 c           86 g           47 t
ORIGIN      
        1 tacgaaggga gcgagcgttg ttcggaatca ctgggcttaa agcgtgcgta ggcggacagg
       61 aaagtcagaa gtgaaatccc ggggctcaac cccggaactg cttttgaaac ttcctgtcta
      121 gaatccggaa gaggttggcg gaatatccag tgtagaggtg aaattcgcag atattggata
      181 gaacaccggt ggcgaaggcg gccaactggt ccggaattga cgctgaggta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014706             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14876 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014706
VERSION     KHUT01014706.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14876"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           86 g           53 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ttgggcgtaa agggcgcgta ggcggttagg
       61 aaagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac ttcctgacta
      121 gagtacaatt agggtaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccggt ggtgaagacg gtttactggg ttgtaactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014707             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14877 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014707
VERSION     KHUT01014707.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14877"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           38 c           80 g           59 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttattg gtcgatgcca aactcaattt ggtggaagct gataatactg ttcggctaga
      121 ggacaggaga ggttgatgga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaaa aggcgaaggc agtcaactat tctgttcctg acattgaggc ataaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01014708             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14878 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014708
VERSION     KHUT01014708.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14878"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           55 c           92 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatca ctgggcgtaa agcgcacgta ggcgggctct
       61 taagtcggtg gtgaaatcct ggggctcaac cccagaactg cctccgatac tgagagtctt
      121 ggagtccggg agaggtgagt ggaactgcga gtgtagaggt gaaattcgta gatattcgca
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgaggt gcgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014709             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14879 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014709
VERSION     KHUT01014709.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14879"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           49 c           94 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggattta ctgggcgtaa agagcgcgca ggcggtcttg
       61 taagtcgaat gtgaaagccc ctggctcaac tggggagggt cattcgatac tgctcgactt
      121 gaaggtagga gagggaagcg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctggc ctattcttga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014710             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14880 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014710
VERSION     KHUT01014710.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14880"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           51 c           84 g           44 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggctttg
       61 caagtcaagt gtgaaatccc ggggctcaac ctcggaacag cacctgaaac tgcagagctc
      121 gaggataggc ggagagaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttctctagc ttaaacctga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014711             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14881 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014711
VERSION     KHUT01014711.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14881"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           39 c           94 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtga ctgggcgtaa agggcatgta ggcggttata
       61 ttagtcagat gtgaaagacc tgggcttaac ctgggatgtg catgtgagac ggtatagcta
      121 gagtttggga gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacactcgt ggcgaaggcg gctttctggc ccaaaactga cgctgaggtg cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014712             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14882 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014712
VERSION     KHUT01014712.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14882"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           54 c           85 g           49 t
ORIGIN      
        1 gacgaaccat ccaaacgtta ttcggaatta ctgggcttaa agggtgcgta ggcggcgcag
       61 taagttgggt gtgaaatccc tcggcttaac cgaggaactg cgcccaaaac tactgtgctt
      121 gagggagaca gaggtgagcg gaacttaggg tggagcggtg aaatgcgttg atatcctaag
      181 gaacacccgt ggcgaaagcg gctcactggg tctcttctga cgctgatgca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014713             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14883 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014713
VERSION     KHUT01014713.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14883"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           53 c           95 g           42 t
ORIGIN      
        1 tacggagggg gccagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggccaag
       61 caagtcggac gtgaaatacc tcggctcaac tggggaaatg catccgaaac tgcctggctt
      121 gagtcctgga gagggtagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctacctgga caggtactga cgctgaggcg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014714             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14884 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014714
VERSION     KHUT01014714.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14884"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           51 c           91 g           44 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtccag
       61 caagtcgggt gtgaaagccc tcggctcaac cgaggaagtg cattcgaaac tactggactt
      121 gaataccgga gagggtgacg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtcacctgga cggatattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014715             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14885 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014715
VERSION     KHUT01014715.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14885"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           51 c           89 g           51 t
ORIGIN      
        1 gacgaactgt cctaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggtccaa
       61 caagtcaggg gtgaaatact acagctcaac tgtagaagtg cctctgatac tgttggtctc
      121 gagggagata ggggtgtgcg gaacttccag tggagcggtg aaatgcgttg atattggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014716             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14886 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014716
VERSION     KHUT01014716.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14886"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           88 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatca ctaggcgtaa agcgcaggta ggtggtcagg
       61 taagtctctt gtgaaagctc ccggctcaac tgggagaggc caagggaaac tacctggcta
      121 gagtgtggta gaggaagctg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaatacctat ggcgaaagca ggcttctggg ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014717             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14887 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014717
VERSION     KHUT01014717.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14887"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           94 g           46 t
ORIGIN      
        1 gacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggcgact
       61 aaagtcagat gtgaaagccc tgggctcaac ccaggaagtg catctgatac tgagttgctt
      121 gagtgctgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg accacctgga cagacactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014718             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14888 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014718
VERSION     KHUT01014718.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14888"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           85 g           50 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgca ggcggtcagg
       61 aaagtcagat gtgaaattcc ggggctcaac cccggagctg catttgaaac ttcctatcta
      121 gagttcagtt agggaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacaccagt ggtgaagacg gttttctggg ctgatactga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014719             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14889 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014719
VERSION     KHUT01014719.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14889"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           92 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcggattgt
       61 taagtcgggg gtgaaagccc agggctcaac cctggaatgg ccttcgatac tggcaatctg
      121 gagaacggga gaggtgagtg gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaacaccagt ggcgaaggcg gctcactggc ccgtttctga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014720             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14890 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014720
VERSION     KHUT01014720.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14890"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           94 g           45 t
ORIGIN      
        1 tacatgtggg gcaagcgttg ttcggaatta ctgggcataa agggtgcgta ggcggcgctg
       61 taagtcaggg gtgaaatccc acggcccaac cgtggaacgg cctttgatac tgcagtgctt
      121 gagtgcgtga ggggtgactg gaacgcgtgg tgtagcggtg aaatgcgtag atatcacgcg
      181 gaaggccaaa ggcgaaggca agtcactggt acgcaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014721             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14891 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014721
VERSION     KHUT01014721.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14891"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           42 c           93 g           51 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ttgggcgtaa agggcgcgta ggcggttatt
       61 taagtgagat gtgaaatccc tgggcttaac ctaggaacag cattttagac tggatgactg
      121 gagtacagta gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg gccacctgga ctgaaactga cgctgaggcg cgagagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014722             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14892 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014722
VERSION     KHUT01014722.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14892"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           86 g           55 t
ORIGIN      
        1 gacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggccatt
       61 caagttagac gtgaaagccc agggcttaac cctggaattg cgtttaatac tgtttggcta
      121 gagttcgata gagggaagta gaattcctag tgtagaggtg aaattcgtag atattaggaa
      181 gaataccaga ggcgaaggcg gcttcctgga tcgatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014723             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14893 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014723
VERSION     KHUT01014723.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14893"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           51 c           99 g           51 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agcgcgcgta ggcggcaagg
       61 tgtgtcgtgc gtaaaatctc ccggcttaac tgggaggcgc cgtgcgaaac tgcctagctt
      121 gaggtcggaa ggggagagtg gaattcccgg tgtagtggtg gaatacgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctctctggt ccgattctga cgctgaggtg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014724             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14894 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014724
VERSION     KHUT01014724.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14894"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           45 c           94 g           50 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtctgt
       61 taagtctgat gtgaaatccc ttggctcaac tgaggaattg cattggaaac tagcgggctg
      121 gaatatcgga gaggagagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaggcg gctctctggc cgaatattga cgctgaggca cgaaagcatg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014725             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14895 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014725
VERSION     KHUT01014725.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14895"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           87 g           50 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggttatt
       61 caagtcaggg gtgaaatcct ggggctcaac cccagaattg cctctgaaac tgtataacta
      121 gagtgtcgga ggggatagcg gaattgctaa tgtagcggtg aaatgcgtag atattagcag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014726             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14896 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014726
VERSION     KHUT01014726.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14896"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           94 g           49 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatga ctgggcgtaa agggcgcgta ggcggtgata
       61 caagtgagat gtgaaatccc tgggcttaac ctaggaatgg cattttagac tgtgtgacta
      121 gaggctagta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacatcagt ggcgaaggcg actacctgga ctggaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014727             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14897 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014727
VERSION     KHUT01014727.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14897"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           92 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggctttg
       61 caagtcgggt gtgaaagccc acggctcaac cgtggaagtg cgcccgaaac tgcagagctt
      121 gagtgtcaaa gaggatggcg gaattcccca agtagaggtg aaattcgtag atatggggag
      181 gaacaccggt ggcgaaggcg gccatctggg tgacaactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014728             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14898 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014728
VERSION     KHUT01014728.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14898"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           94 g           47 t
ORIGIN      
        1 tacggagggg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcctag
       61 aaagtcagat gtgaaagccc ttggcttaac caaggaactg catctgaaac tgcttggctt
      121 gaattcggga agggagagtg gaattcccag tgtagcggtg aaatgcgtag atactgggag
      181 gaacaccggt ggcgaaggcg gctctctgga ccgatattga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014729             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14899 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014729
VERSION     KHUT01014729.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14899"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           85 g           59 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ttgggcgtaa agcgaacgta ggcggtttct
       61 taagtttggt cttaaagact ggggctcaac ctcaggagtg gactgaatac tgggagacta
      121 gaggatgtta gaggaaagtg gaattccaag tgtagcggtg aaatgcgtag atatttggag
      181 gaacaccagt ggcgaaagcg gctttctggg acattcctga cgctgaggtt cgaaagctcg
      241 gggagcaaac agg
//
LOCUS       KHUT01014730             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14900 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014730
VERSION     KHUT01014730.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14900"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           40 c           79 g           58 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggaatta ttgggcgtaa aggatgcgta gatggtagaa
       61 taagttactg gttgatgcca aactcaattt gacggaagct ggtaatacta ttctgctaga
      121 gggcaggaga agttgatgga attctgtgtg gagcggtgaa atgcgttgat ctacagagga
      181 acaccaaaaa aggcgaaggc agtcaaccat tctgttcctg acattgaggc atgaaagcgt
      241 ggggagcaaa ccgg
//
LOCUS       KHUT01014731             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14901 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014731
VERSION     KHUT01014731.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14901"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           46 c           95 g           48 t
ORIGIN      
        1 tacggggggg gcaagcgttg ttcggaatta ctgggcgtaa agggtgcgta ggcggtgcgc
       61 taagttggat gtgaaaactc tgggcttaac ccagagactg cattcaaaac tgacgtgcta
      121 gagttctgga gggggtagcg gaattcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacaccggt ggcgaaggcg gctacctgga cagagtctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014732             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14902 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014732
VERSION     KHUT01014732.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14902"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           85 g           52 t
ORIGIN      
        1 tacgaaggga gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcctga
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaactg catttgatac tgtcaggcta
      121 gagatcgaga gaggaaagtg gaattacgag tgtagaagtg aaattcgtag atattcgtag
      181 gaacacctgt ggcgaaggcg actttctggc tcgatactga cgctaaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014733             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14903 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014733
VERSION     KHUT01014733.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14903"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           86 g           52 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggcgttg
       61 ttagtcttct gtcaaattct tcggcttaac cgggggcagg caggggaaac ggcaatgctc
      121 gaggatgcga ggggtgaatg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa agcgaaggca attcactggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014734             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14904 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014734
VERSION     KHUT01014734.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14904"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           89 g           48 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggccaag
       61 caagtccgat gtgaaatccc agggctcaac cctggaactg cattggaaac tgctaggctt
      121 gagtaccgga gaggtgaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg cctcactggc cggttactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014735             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14905 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014735
VERSION     KHUT01014735.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14905"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           55 c           87 g           48 t
ORIGIN      
        1 gacgaaccgt ccaaacgtta ttcggaatca ctgggcttac agagttcgta ggcggtccgg
       61 taagttaggt gtgaaatccc tcggctcaac cgaggaactg cgcttaatac tgccggactt
      121 gagggggata gaggagagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaggcg gctctctggg tccctcctga cgctgaggaa cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014736             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14906 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014736
VERSION     KHUT01014736.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14906"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           51 c           84 g           47 t
ORIGIN      
        1 gacagaggat gcaagtgtta tccgaaatga ttgggcgtaa agcgtctgtt ggtggctttt
       61 caagtccgtc gtcaaatccc agggctcaac cctggacagg cggtggaaac taccaagctg
      121 gagtacggta ggggcagatg gaatttccgg tggagcggtg aaatgcattg agatcggaaa
      181 gaacaccaac ggcgaaagca ctctgctggg ccgacactga cactgagaga tgaaagctag
      241 gggagcaaat ggg
//
LOCUS       KHUT01014737             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14907 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014737
VERSION     KHUT01014737.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14907"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           50 c           99 g           49 t
ORIGIN      
        1 gacgtagggt gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcgt
       61 tgcgtcagct gtgaaaatcc acggcttaac cgtgggcctg cagctgatac gggcggacta
      121 gagttcggta ggggagactg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg ggtctctggg ccgatactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014738             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14908 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014738
VERSION     KHUT01014738.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14908"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           96 g           45 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatta ctgggcgtaa agggcgcgta ggcggcttgg
       61 caagttgggc gtgaaaggcc tgggcttaac ccgggaaatg cgttcgatac tgccgagcta
      121 gagtgtggga gaggatggcg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccagt ggcgaaggcg gccatctgga ccacaactga cgctaaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014739             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14909 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014739
VERSION     KHUT01014739.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14909"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           86 g           50 t
ORIGIN      
        1 tacggagggt gctaacgttg tccggaatta ctgggcgtaa agcgcatgta ggtggtcttg
       61 cacgtcgacg atgaaagact gtcgcctaac gacagaaacg ttttcgatac gacaaggctt
      121 gaggggagca ggggtacgcg gaactttcgg tgtaggagtg aaatccgtag atatcgaaag
      181 gaacaccaat ggcgaaggca gcgtactggg cttttcctga cactgagatg cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014740             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14910 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014740
VERSION     KHUT01014740.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14910"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           51 c           86 g           52 t
ORIGIN      
        1 aacgtaggta ccgagcgtta tccggattca ctgggcgtaa agcgtgtgca ggtggtttgg
       61 taagtcgggc atgaaaactc ttggctcaac tgagagacgc tgttcgatac tgtcaaactt
      121 gagggtgtga gagggaggtg gaattccgca tgtagtggtg aaatgcgtag atatgcggag
      181 gaacaccagt ggcgaaggcg gcctcctggc acactcctga cactcataca cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014741             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14911 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014741
VERSION     KHUT01014741.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14911"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           48 c           86 g           53 t
ORIGIN      
        1 tacgagggct ccaagcgttg ttcggaatca ttgggcgtaa agcgagtgcg ggtggcttct
       61 taagtcagga gtgaaagccc agggctcaac cctggaagtg cttttgatac tgggaagctt
      121 gagtgtggga gagggtgcta gaatacctaa tgtagtggtg aaatacgtag atattaggtg
      181 gaacaccggt ggcgaaggcg ggcacctggc ccaacactga cacttagact cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014742             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14912 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014742
VERSION     KHUT01014742.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14912"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           48 a           59 c          107 g           39 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggcggctcgt
       61 caagcgtgcg gtgaaagccc ggggctcaac tccgggtcgg ccgtgcgaac tggcgggctt
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagttgctga cgctgatgcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014743             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14913 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014743
VERSION     KHUT01014743.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14913"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           91 g           46 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggttctg
       61 caagtcggac gtgaaagccc cgggctcaac ccgggaagtg cgctcgatac tgctgaactt
      121 gagtgtcgga gaggttggta gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaataccggc ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014744             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14914 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014744
VERSION     KHUT01014744.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14914"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           50 c           77 g           53 t
ORIGIN      
        1 tacagagacc ccaagcgtta tccggaatta ctgggcgtaa agcgcaagta gtcggttatg
       61 atagtctgat gttaaatttc atcgctcaac ggtgaagccg cattagaaac gtcattacta
      121 gagcgagtca ggggcacgcg gaacgtgtgg tgtagcggtg aaatgcattg atatcacacg
      181 gaacaccaaa agcgaaggca gcgtgctggg gctttgctga cgatcagttg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01014745             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14915 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014745
VERSION     KHUT01014745.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14915"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           39 c           79 g           60 t
ORIGIN      
        1 tacgagaggt gcaagcgtta ttcggaatca ttgggcgtaa agggtgcgta gacggtatgt
       61 taagtctgtt gttaaatctt tcggcttaac cgaaaatctg caatggaaac tggcatgcta
      121 gagtatggaa gagataagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttattggt ccattactga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014746             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14916 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014746
VERSION     KHUT01014746.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14916"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           49 c           89 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatta ctgggcgtaa agcgtgtgta ggcggtctcg
       61 taagtggggt gtgaaagccc taggctcaac ctaggaactg cgccccaaac tgcgagactt
      121 gagtgtcgga gaggatagtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccagt ggcgaaggcg gctatctgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014747             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14917 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014747
VERSION     KHUT01014747.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14917"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           55 c           91 g           46 t
ORIGIN      
        1 tacgaagggg gctagcgttg ttcggaatca ctgggcgtaa agcgtatgca ggcggaccgt
       61 taagtcgggg gtgaaatccc agggctcaac cctggaactg ccttcgatac tggcggcctt
      121 cgagtccggg agaggtgagt ggaactccga gtgtagaggt gaaattcgta gatattcgga
      181 agaacaccag tggcgaaggc ggctcactgg cccggtactg acgctgagat acgaaagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014748             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14918 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014748
VERSION     KHUT01014748.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14918"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           42 c           86 g           59 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcaatg
       61 taagttggat gtgaaatctt gaggcttaac ctcaaaattg cattcaatac tgcgttgctt
      121 gagcgctgta gaggagattg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaagaccagt ggcgaaggcg aatctctggg cagctgctga cgctgaggca cgaaggccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014749             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14919 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014749
VERSION     KHUT01014749.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14919"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           40 c           92 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaat
       61 caagttagct gtgaaatacc agggcttaac cttggggtga gagctaagac tggttgactt
      121 gagtatcgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaggcg gctacctgga cgaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014750             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14920 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014750
VERSION     KHUT01014750.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14920"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           87 g           48 t
ORIGIN      
        1 tacggagggt gcaagcattg atcggaatta ctgggcgtaa agggcgcgta ggcggccaag
       61 aaagtcagat gtgaaatacc ggggctcaac cccggtgcgg catttgaaac ttcatggctc
      121 gagttcagtt agggaaaaca gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaataccggt ggtgaagacg gttttctggg ctgaaactga cgctgaggcg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014751             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14921 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014751
VERSION     KHUT01014751.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14921"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           84 g           55 t
ORIGIN      
        1 tacagaagcc tcgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggcggtgacg
       61 taagtcagat gtgaaagccc tgggcttaac ccgggaagtg catttgatac tgcgttgcta
      121 gagtgcgaaa gaggaaagtg gaattcccgg tgtaggagtg aaatccgtag atatcgggag
      181 gaataccaat ggcgaaggca gctttctggt tcgtaactga cgctgagatt cgaaagcatg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014752             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14922 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014752
VERSION     KHUT01014752.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14922"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           46 c           82 g           53 t
ORIGIN      
        1 tacgaagggg gcgagcgtta ttcggaatca ctgggcgtaa agcgtgcgta ggctgttttg
       61 taagttggaa gtgaaagccc agggcttaac cctggaattg ctttcaaaac tacaagactc
      121 gaatctcgga gaggatagcg gaattgtcag tgtagcagtg aaatgcgtag atattgacag
      181 gaacaccagt ggcgcaagcg gctatctgga cgagtattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014753             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14923 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014753
VERSION     KHUT01014753.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14923"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           45 c           99 g           53 t
ORIGIN      
        1 tacgtaggat gcgagcgttg tccggagtta ttgggcgtaa agagcacgta ggcggtgcga
       61 taagtgtggt gtgaaatctg ggggcttaac tcccagcggt cgctgcagac tgtcgtgctg
      121 gagggctgga gaggaagata gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gtcttctggc cagttcctga cgctgaggtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014754             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14924 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014754
VERSION     KHUT01014754.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14924"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           41 c           82 g           53 t
ORIGIN      
        1 tacggagagt gcaagcatta atcggaatta ttgggcgtaa agggcgcgta ggcggaaagg
       61 taagtcagat gtgaaatact gaagctcaac ttcagtgctg catttgaaac tgcaattcta
      121 gagggttgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagt tgatacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014755             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14925 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014755
VERSION     KHUT01014755.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14925"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           93 g           48 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ctgggcgtaa agggcgtgta ggcggtggag
       61 tgtgtttggt gtgaaagccc tcggcttaac tgaggaagtg cattgaaaac tactctgcta
      121 gagtgcggga gaggatggtg gaattcccaa agtagaagtg aaattcgtag agattgggag
      181 gaacacctga ggcgaaggcg gccatctggc ccgcaactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014756             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14926 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014756
VERSION     KHUT01014756.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14926"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           81 g           58 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggcggctttg
       61 taagtcagtg gtgaaagctc cccgcttaac ggggaaactg ccattgatac tgcttagctt
      121 gagtacagtt gaggtgggcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccgat tgcgaaggca gctcgctaaa ctgtaactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014757             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14927 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014757
VERSION     KHUT01014757.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14927"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           50 c           88 g           46 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcctcg
       61 taagtcaaat gtgaaagccc tcggctcaac cgaggaagcg catttgatac tgcgaagctt
      121 gagtatcgga gaggagagcg gaacacccgg tgtagaggtg aaattcgtag atatcgggta
      181 gaacaccagt ggcgaaggcg gctctctgga cgaatactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014758             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14928 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014758
VERSION     KHUT01014758.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14928"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           46 c           81 g           51 t
ORIGIN      
        1 tacgtagggt gcaagcatta atcggaatta ttgggcgtat agggcgcgta ggcggatcta
       61 taagtcggtt gtgaaatccc agggctcaac cctggaatag cacccgaaac tatagaacta
      121 gagggtaggc ggagaaaacg gaattccaca agtagcggtg aaatgcgtag atatgtggaa
      181 gaacatcggt ggcgaaggcg gttttctagc ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014759             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14929 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014759
VERSION     KHUT01014759.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14929"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           44 c           81 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgtta gtcggaatta ctgggcgtaa agggtgcgta ggcggaaaca
       61 taagtcagat gtgaaaaacc aaagctcaac tttggcagtg catctgaaac tgtgtttcta
      121 gaggaagagc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc ttactcctga cgctgaggca cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014760             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14930 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014760
VERSION     KHUT01014760.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14930"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           92 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtgat
       61 caagtctatg gtgaaatact ccagcccaac tggagaactg ccgtggatac tgattgtctc
      121 gagggaggta ggggcgtgcg gaactgttag tggagcggtg aaatgcgttg atattaacag
      181 gaactccggt ggcgaaggcg gcacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014761             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14931 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014761
VERSION     KHUT01014761.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14931"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           90 g           54 t
ORIGIN      
        1 tacgtagggt gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggcttag
       61 caagtcggaa gttaaaacaa gaggctcaac ctcttgtcgc tttcgatact gctatggctt
      121 gagtctggta ggggatcacg gaattccacg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gtgatctggg ccagtactga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014762             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14932 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014762
VERSION     KHUT01014762.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14932"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           49 c           94 g           45 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggcggcttgg
       61 aaagtcggat gtgaaagccc agggctcaac cttggaagtg catccgaaac tgctgagctc
      121 gaatcccgga gagggtggtg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg accacctgga cggagattga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014763             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14933 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014763
VERSION     KHUT01014763.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14933"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           92 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcccga
       61 taagtctgat gtgaaatctt gaggctcaac ctcaagcggt cattggatac tgtcgggcta
      121 gagtacggaa gaggagagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacacctat ggcgaaggca gctctctggt acgagactga cgctgagacg cgaaagcatg
      241 gggagcgaac agg
//
LOCUS       KHUT01014764             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14934 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014764
VERSION     KHUT01014764.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14934"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           51 c           81 g           53 t
ORIGIN      
        1 tacatagacc tcaagcgtta tccggattta ctgggcgtaa agcgtgcgta ggtgtccttt
       61 taagtcctcg gtgaaatctc tccgcctaac ggagaaactg tcgaggaaac tggaaggatt
      121 gagtgtgaca gaggctgacg gaattcgagg tgtagcggta aaatgcgtag atatctcgaa
      181 gaacaccagt ggcgaaggcg gtcagctggg tcactactga cactgaggca cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014765             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14935 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014765
VERSION     KHUT01014765.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14935"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           87 g           56 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agagtatgta ggcggatatt
       61 taagtcagat gtgaaattcc cgggcttaac ctgggcgctg catttgaaac tgggtatctg
      121 gagtgcggga gaggaaagtg gaattcctag tgtagcggtg aaatgcgtag agattaggaa
      181 gaacaccagt ggcgaaggcg actttctgga ccgtaactga cgctgagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014766             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14936 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014766
VERSION     KHUT01014766.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14936"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           84 g           58 t
ORIGIN      
        1 tacgagtggt gcaagcgtta ttcggattta ttgggcgtaa agggcgcgta gacggtatga
       61 taagtctgtt gttaaatccc tcggcttaac cgaggatctg cgatggaaac tgttgtgcta
      121 gaggatggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccaat ggcgaaggca gcttcttggt ccactcctga cgttgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014767             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14937 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014767
VERSION     KHUT01014767.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14937"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           40 c           82 g           59 t
ORIGIN      
        1 tacgggggga gctagcgttt ttcggaatta ctgggcgtaa agggcgcgta ggcggttagt
       61 aaagttgaaa gtgaaatccc cgagcttaac tcgggaactg ctttcaaaac tcattaacta
      121 gaggatgata gaggatagta gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaataccggt ggcgaaggcg gctatctgga tcatttctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014768             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14938 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014768
VERSION     KHUT01014768.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14938"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 tacagaagcc tcgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggcggtgacg
       61 taagtcagat gtgaaagccc cgggcttaac ctgggaagtg catttgatac tgcgttgcta
      121 gagtgtgtga gaggaaagcg gaattcccag tgtaggggtg aaatccgtag atattgggag
      181 gaataccgat ggcgtaggca gctttctggc acataactga cgctgagatt cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014769             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14939 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014769
VERSION     KHUT01014769.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14939"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           88 g           54 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggcggtttga
       61 taagtcggtc gtgaaatccc tgggcttaac ctaggaactg cgatcgaaac tattagactt
      121 gagtatcgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga agattactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014770             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14940 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014770
VERSION     KHUT01014770.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14940"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           85 g           59 t
ORIGIN      
        1 tacgtggggt gcaagcgtta tccggattta ttgggcgtaa agagtttgta ggcgtcttat
       61 caagttttgc ttgaaatcct ggggctcaac cccagaactg ggtgaaatac tggtgagatt
      121 gagtctgtta gggggtactg gaactcatgg tgtagcagtg aaatgcgttg atatcatgag
      181 gaacaccaag ggcgaaggca ggtacctggg acatgactga cgctgagaaa cgaaagctag
      241 gggagcgaaa ggg
//
LOCUS       KHUT01014771             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14941 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014771
VERSION     KHUT01014771.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14941"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           37 c           86 g           55 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggcggataaa
       61 taagttagat gtgaaatacc tgagcttaac ttaggaggtg catttaagac tgtagatcta
      121 gagtatgtca gaggggggta gaattccaag tgtagcagtg aaatgcgtag agatttggag
      181 gaataccgat ggcgaaggca gccccctggg atgatactga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014772             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14942 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014772
VERSION     KHUT01014772.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14942"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           45 c           87 g           54 t
ORIGIN      
        1 gacgaggggt ccaagcgttg ttcggaatta ttgggcgtaa agcgggtgta ggcggcctta
       61 taagtcagaa gtgaaagccc caggcttaac ctgggaagtg cttttgatac tgcaaggctt
      121 gaatgtggga gaggatagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacatcggt ggcgaaggcg gctatctggc ccaacattga cgctcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014773             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14943 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014773
VERSION     KHUT01014773.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14943"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           97 g           42 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ttcggattca ctgggcataa agggcgcgta ggcggttccg
       61 aaagtggcat gtgaaatccc ccggctcaac cggggaagtg cgtgccaaac ttcggtgcta
      121 gagtgcggta ggggggactg gaactctcgg tggagcggtg aaatgcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg agtccctggt ccgcaactga cgctgaggcg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014774             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14944 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014774
VERSION     KHUT01014774.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14944"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           57 c           90 g           43 t
ORIGIN      
        1 gacagaggtc ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacccg
       61 taagtacctt gtgaaatccc ccggctcaac cggggaacgg ctcggtatac tgcgggtctt
      121 gagcaaccta ggggcgagcg gaacaagtgg tggagcggtg aaatgcgtag atatcacttg
      181 gaacgccaat ggtgaaaaca gctcgctggg ggtttgctga cgctgagaca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014775             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14945 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014775
VERSION     KHUT01014775.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14945"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           42 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ttgggcgtaa agggagcgta ggcggatgtg
       61 caagtggtag gtgaaatacc cgggcttaac ctggggaggt cctatcagac tgcacgtcta
      121 gaatgtggta gaggaaggtg gaattcctgg tgtagcggtg aaatgcgtag atatcaggaa
      181 gaacatcaat ggcgaaggca accttctggg ccattattga cgctgaggct cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014776             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14946 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014776
VERSION     KHUT01014776.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14946"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           56 c           97 g           41 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggaccgt
       61 taagtacctt gtgaaatccc acggctcaac cgtggaacgg ctgggtagac tggcggtctt
      121 gagcgaccca ggggtcaccg gaacaagcgg tggagcggtg aaatgcgtag atatcgcttg
      181 gaacgccggc ggtgaaaacg ggtgactggg ggtctgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014777             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14947 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014777
VERSION     KHUT01014777.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14947"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           52 c           94 g           47 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggcttgc
       61 aaagtcaggg gtgaaagcct ccagcttaac tggagaagtg cctttgatac tggcaagctc
      121 gagggaggta ggggcagatg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg atctgctgga cctcttctga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014778             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14948 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014778
VERSION     KHUT01014778.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14948"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           44 c           88 g           55 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ttgggcgtaa agggtgcgta ggcggcggag
       61 taagtgggat gtgaaatccc ggagcttaac ttcggaattg cattcgatac tgctttgcta
      121 gagtgctgga gaggagactg gaatttacgg tgtagcagtg aaatgcgtag atatcgtaag
      181 gaacaccagt ggcgaaggcg agtctctgga cagctactga cgctaaggca cgaaggccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014779             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14949 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014779
VERSION     KHUT01014779.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14949"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           49 c           94 g           47 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggtagtc
       61 catgtcgggt gtgaaagccc tcagcttaac tggggaagtg cgcccgaaac tggactgcta
      121 gagtgtcgga ggggaaggcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccttctgga cgaacactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014780             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14950 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014780
VERSION     KHUT01014780.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14950"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           40 c           82 g           58 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggaatta ttgggcgtaa agggcgtgta ggcggatttg
       61 taagtcagat gtgaaatacc gaggcttaac ttcggtgctg catttgaaac tacaaatcta
      121 gaggattgac ggagaaaacg gaattccacg tgtagcggtg aaatgcgtgg atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagt ttattcctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014781             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14951 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014781
VERSION     KHUT01014781.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14951"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           98 g           44 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggtcga
       61 caagtccggg gtgaaagccc ccagcttaac tggggaagtg ccttggaaac tgtcggtcta
      121 gagggaggta ggggcatgtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg acgtgctgga tctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014782             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14952 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014782
VERSION     KHUT01014782.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14952"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           94 g           43 t
ORIGIN      
        1 gacgaaccgt ccaaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcggatgct
       61 caagtcggtg gtgaaatctt tcggctcaac cggaaaagtg ccgccgatac tggggatctc
      121 gagggaggta ggggtcgagg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg ctcgactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014783             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14953 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014783
VERSION     KHUT01014783.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14953"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           48 c           89 g           49 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtttta
       61 taagccagat gtgaaagccc cgggctcaac ctgggaatgg catttgggac tgtaaggctg
      121 gagtatggca gaggggacta gaattcctgg tgtagcagtg aaatgcgtag atatcaggag
      181 gaataccgat ggcgaaggca ggtccctggg ccaatactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014784             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14954 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014784
VERSION     KHUT01014784.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14954"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           42 c           88 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttatt
       61 taagtttgat gtgaaagccc tgggcttaac ctaggaattg cattgaatac tgaataactg
      121 gagtactgca gagggcagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actgcctgga cagatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014785             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14955 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014785
VERSION     KHUT01014785.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14955"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           87 g           55 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcacgta ggtggatatt
       61 taagtcggat gtgaaagccc tgggcttaac ctaggaactg catttgatac tgaatgtcta
      121 gagtatggta gaggaaagtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccaatactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014786             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14956 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014786
VERSION     KHUT01014786.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14956"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           48 c           96 g           48 t
ORIGIN      
        1 gacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcttgta ggcgggatgg
       61 cgcgtcgtgg agtaaatctc tcggctcaac tgagagaaca tctgcgatac ggccgttctt
      121 gaggacagaa gaggaaagca gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaataccagt ggcgaaggcg gctttctggt ctgattctga cgctgaggag cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014787             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14957 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014787
VERSION     KHUT01014787.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14957"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           81 g           58 t
ORIGIN      
        1 tacgtagggt ccaagcatta tccggattta ttgggcgtaa agaacatgta ggtggtttgg
       61 taagtcacgt cttaaagctc tgggctcaac ccagaatctg gacgagatac tgtcagactt
      121 gtggtcagca ggggcaaatg gaattcccgg tgtaggagtg aaatccgttg atatcgggag
      181 gaacaccaat ggcgaaggca atttgctagg ctgattcaga cactgagatg tgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014788             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14958 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014788
VERSION     KHUT01014788.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14958"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           51 c           98 g           43 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agcgcgcgta ggcggtcttc
       61 taagttgggt gtgaaagccc ggagcttaac tccggaaatg cgcccaagac tggaagactg
      121 gagtccggga gaggtgggtg gaactcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcccactgga ccggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014789             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14959 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014789
VERSION     KHUT01014789.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14959"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           61 c          102 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcgtc
       61 atagtccgcg gtgaaatccc ccggctcaac cgggggacgg ccgcggatac taggcgtctg
      121 gaggcgggta ggggcgtgcg gaacactagg tggagcggtg aaatgcgttg atatctagtg
      181 gaactccggt ggcgaaagcg gcgcgctgga cccgatctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014790             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14960 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014790
VERSION     KHUT01014790.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14960"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           86 g           57 t
ORIGIN      
        1 tacgaggggt gcaagcgtta ttcggagtta ctgggcgtaa agagtgcgta ggcggtctgc
       61 taagttgttt gttaaatttc tcggcttaac tgaggatagg caagcaaaac tggtgaacta
      121 gaggacaaga gagagaggtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaatatcggt tgcgaaggcg gcctcttggc ttgtatctga cgctaaagca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014791             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14961 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014791
VERSION     KHUT01014791.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14961"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           45 c           89 g           51 t
ORIGIN      
        1 tacgaagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggtttga
       61 taagcgatat gtgaaagccc tgggctcaac ctaggaactg catgtcgaac tgtcaggcta
      121 gagtatggta gaggggtgtg gaatttcctg tgtagcggtg aaatgcgtag atataggaag
      181 gaacatcagt ggcgaaggcg acaccctgga ccgatactga cactgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014792             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14962 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014792
VERSION     KHUT01014792.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14962"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           42 c           72 g           64 t
ORIGIN      
        1 tacgaaggtc ccgagcgtta tccggattca ttgggtttaa agggtgcgta ggtggaatga
       61 taagtcagtg gtgaaatctt gcagcttaac tgtaaaactg ccattgatac tgtcgttctt
      121 gagtatagtt gatgtgggcg gaatatgtca tgtagtggtg aaatacttag atatgacata
      181 gaacaccgat tgcgaaggca gctcacaaag ctacaactga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014793             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14963 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014793
VERSION     KHUT01014793.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14963"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c           97 g           45 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agcgcgcgta ggcggcttgg
       61 taagtcggtt gtggaagcct cgagctcaac tcgggaacgg cgaccgaaac tgccagactt
      121 gagtacggta ggggatgctg gaattcctgg tgtagcggtg aaatgcatag atatcaggag
      181 gaacaccggt ggcgaaggcg ggcatctggg ccgttactga cgctgaggcg cgaaagctag
      241 gggagcaaac cgg
//
LOCUS       KHUT01014794             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14964 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014794
VERSION     KHUT01014794.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14964"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           59 c           94 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcggg
       61 caagtctgag gtgaaatccc acggctcaac cgtggaagag ccttcgatac tacccgtctc
      121 gagggaggta ggggcgtgtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg acacgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014795             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14965 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014795
VERSION     KHUT01014795.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14965"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           46 c           85 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattca ttgggcgtaa agggtgcgta gatggcgccg
       61 taagtcgggt gtgaaatctc ggggcttaac tccgaaactg cattcgatac tgcggtgctt
      121 gaggactgga gaggaaactg gaatttatgg tgtagcagtg aaatgcgtag agatcataaa
      181 gaagaccaat ggcgaaggcg ggtctctgga cagttcctga cactgaggca cgaaggccaa
      241 aggagcaaac ggg
//
LOCUS       KHUT01014796             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14966 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014796
VERSION     KHUT01014796.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14966"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           49 c           81 g           51 t
ORIGIN      
        1 cacgagagcc tcaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggtaatg
       61 ttagtctcat gtcaaagcct ctggcttaac cagagatccg catgggaaac ggcataacta
      121 ggagggtgca agaggtgaat ggaactcacg gtgtaggggt gaaatccgtt gatatcgtgg
      181 ggaacgccaa aagcgaaggc aattcactgg tgcatacctg acgctgaaac acgaaagcca
      241 gggtatcgaa tggg
//
LOCUS       KHUT01014797             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14967 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014797
VERSION     KHUT01014797.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14967"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           88 g           48 t
ORIGIN      
        1 tacgaagggt gcaaacgttg ctcggaatta ttgggcgtaa ggcgcacgta ggcggcttag
       61 caagttggat gtgaaagccc tcggcttaac caaggaagtg catccaaaac tgctgagctt
      121 gagtagttaa gaggatcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgatctggg aacatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014798             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14968 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014798
VERSION     KHUT01014798.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14968"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           91 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggtggcttgg
       61 taagtccggt gtgaaacctt ggggctcaac cccaagcctg catcggatac tgcctcgctt
      121 gagttcggga ggggagagcg gaattcctag tgtagcggtg aaatgcgcag atattaggaa
      181 gaacaccggt ggcgaaggcg gctctctgga acgatactga cactgagacg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014799             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14969 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014799
VERSION     KHUT01014799.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14969"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           39 c           85 g           59 t
ORIGIN      
        1 tacagaggcc tcgagcgttg ttcggaatta ctgggcgtaa agggaatgta ggtggtgata
       61 caagtcagat gtgaaagccc tgggcttaac ctgggaagtg catttgatac tgtattgctg
      121 gagtgcgaga gaggaaagtg gaattccaag tgtaggggtg aaatccgtag atatttggag
      181 gaataccgat ggcgaaggca gctttctggc tcgtaactga cactgagatt cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014800             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14970 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014800
VERSION     KHUT01014800.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14970"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           92 g           53 t
ORIGIN      
        1 tacgaagggt gcgagcgttg ttcggaatta ttgggcgtaa agggttcgta ggcggttatg
       61 caagtcaagt gtgaaaaccc tgggctcaac cttgggcgtg catttgaaac tgcgtatctt
      121 gagttttgga gagggtggtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacaccgga ggcgaaggcg accacctggc cgtagactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014801             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14971 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014801
VERSION     KHUT01014801.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14971"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           43 c           85 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggtaaag
       61 taagttaggt gtgaaatcct caggctcaac ctgagaactg cgcttaagac tgctttacta
      121 gagtactgta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg acttcctgga cagatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014802             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14972 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014802
VERSION     KHUT01014802.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14972"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           46 c           89 g           55 t
ORIGIN      
        1 gacgaggggt cctagcgttg ttcggaatta ttgggcgtaa agcgcatgta ggcggctatt
       61 taagtcaggt gtgaaatccc cgggctcaac ccgggaagtg catctgatac tggatagctt
      121 gagtactgga gagggtagtg gaattcctgg tgtagtggtg aaatacgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gctacctggc catgtactga cgctgagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014803             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14973 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014803
VERSION     KHUT01014803.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14973"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           45 c           80 g           59 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggattag
       61 taagtccgtg gtgaaatccc cgagcttaac ttgggaactg ccatggatac tattagtctt
      121 gaatactgtg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacaccaat tgcgaaggca gctcgctaca cggttattga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014804             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14974 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014804
VERSION     KHUT01014804.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14974"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           46 a           58 c          107 g           42 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattca ttgggcgtaa agcgctcgca ggcggttcgc
       61 cgcgtcttgc gtgaaggccc tcggctcaac cgagggtggt cgtaagatac gggcggactg
      121 gaggtaggta gggggacgcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccggc ggcgaaggcg gcgtcctgga cctggcctga cgctcaggag cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014805             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14976 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014805
VERSION     KHUT01014805.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14976"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           91 g           46 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggattca ctgggcgtaa agggcacgta ggtggtcgag
       61 taaatcagct gtggaatacc gtcgctcacc ggcggaattg cagctgaaaa tgctcgtctt
      121 gagcgcagga ggggttagag gaactctagg tggagcggtg aaatgcgtag atatctagag
      181 gaacgccgga ggcgaaagcg tctaactggc ctgctgctga cactgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014806             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14977 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014806
VERSION     KHUT01014806.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14977"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           54 c          103 g           44 t
ORIGIN      
        1 tacgtaggag gcgagcgttg tccggattta ctgggcgtaa agcgcgtgta ggtggcgctg
       61 taagtctgct gtgaaagctc tcggctcaac cgggagaggt cggtggaaac tgcggcgcta
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggccag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014807             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14978 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014807
VERSION     KHUT01014807.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14978"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           48 c           93 g           49 t
ORIGIN      
        1 aacgtaggag gcgagcgtta tccggattta ctgggtgtaa agcgcgtgca ggcggtcggc
       61 taagttggat gtgaaagctc ctggctcaac tgggagaggt cgttcaatac tggcggactt
      121 gagagcagta ggggaaggtg gaattccggg agtagtggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaagcg gccttctgga ctgcatctga cgctcagacg cgaaagctag
      241 ggtagcaaac ggg
//
LOCUS       KHUT01014808             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14979 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014808
VERSION     KHUT01014808.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14979"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           53 c          101 g           41 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ctgggcgtaa agggtgcgta ggcggcttgg
       61 caagtcctgt gtgaaaggtc gcggctcaac cgcggtgggt catgggaaac tgccaagctc
      121 gagtgccgga gaggatagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctatctgga aggtcactga cgctgaggca cgacagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01014809             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14980 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014809
VERSION     KHUT01014809.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14980"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           45 c           84 g           52 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggattta ttgggcgtaa agcgcgcgca ggctgattgg
       61 taagtcaaat gtgaaatctc ggggcttaac cccgaaattg cgtctgaaac tgtcgatcta
      121 gaattctgga gagggaaggg gaatttctca tgtaggggta aaatccgtag agatgagaag
      181 gaacaccaga ggcgaaggcg ccttcctaga cagatattga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014810             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14981 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014810
VERSION     KHUT01014810.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14981"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           46 c           92 g           49 t
ORIGIN      
        1 tacgtagggg gcaagcgtta tccggattta ctgggtgtaa agggagtgta ggcggtctgg
       61 caagtcagat gtgaaagccc ggggctcaac cccgggactg catttgaaac tgccggacta
      121 gagtgtcgga gaggtaagtg gaattcctag tgtagcggtg aaatgcgtag atattaggag
      181 gaacaccagt ggcgaaggcg gcttactgga cgataactga cgctgaggct cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014811             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14982 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014811
VERSION     KHUT01014811.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14982"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           92 g           50 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggcaggta ggtggtctta
       61 tttgtctgat gtgaaagcct tgagcttaac tcaagaagtg cgtcagaaac ggtgagacta
      121 gagtgctgga gaggttcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgaactgga cagtgactga cactcaactg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014812             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14983 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014812
VERSION     KHUT01014812.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14983"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           53 c           84 g           52 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggaatca ctgggcgtaa agggtgcgca ggccgcacgg
       61 gaagtcaaat gtgtaatcca ggggctcaac ccctgaactg cgcttgatac ttccgtgcta
      121 gaaccttgga gaggagtctg gaattctcgg tgtagcagtg aaatgcgtgg atatcgagaa
      181 gaacacttag ggcgtaggca ggactctgga caagtgttga cgctcatgca cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014813             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14984 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014813
VERSION     KHUT01014813.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14984"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           56 c           95 g           45 t
ORIGIN      
        1 gacgaaccga gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggctgt
       61 caagtctggg gtgaaatccc gcggctcaac cgtggaactg ccttagatac tgacggcctc
      121 gaggaaggta ggggcatgcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaactccggt ggcgaaggcg gcgtgctgga ccttttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014814             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14985 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014814
VERSION     KHUT01014814.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14985"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           95 g           50 t
ORIGIN      
        1 tacggaaggt gcgagcgtta atcggaatta ctgggcgtaa agcgcacgta ggcggttact
       61 taagttggat gtgaaagccc tgggcttaac ctgggaattg catctgatac tgggtgacta
      121 gagtacggta gaggggggtg gaattccagg tgtagcggtg aaatgcgtag agatctggag
      181 gaacaccggt ggcgaaggcg gccccctgga ctgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014815             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14986 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014815
VERSION     KHUT01014815.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14986"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           97 g           50 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcggtcctg
       61 tgagtcaggc gtgaaattcc cgggcttaac ctggggactg cgcttgatac tgcgggactt
      121 gaggatggaa gagggtcgtg gaattcccag tgtagaggtg aaattcgtag atattgggaa
      181 gaacaccggt ggcgaaggcg gcgacctggt ccatttctga cgctgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014816             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14987 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014816
VERSION     KHUT01014816.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14987"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           88 g           51 t
ORIGIN      
        1 tacgtggagt ccaagcgtta tccggattta ctgggcgtaa agcgttcgta ggcgtccggc
       61 caagtttccg gtgaaatccc acggcctaac cgtggaattg tcgggaaaac tggtcggatt
      121 gagtatgaca gaggcagagg gaattcccgg tgtaggggta aaatccgttg atatcgggaa
      181 gaacaccagt ggcgaaggcg ctctgctggg tcatcactga cgctgaggga cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014817             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14988 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014817
VERSION     KHUT01014817.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14988"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           51 c           96 g           50 t
ORIGIN      
        1 tacgtaggtg acaagcgttg tccggattta ctgggcgtaa agggcgtgta ggcggttcgt
       61 taagtctgtt gtgaaatctc ccggcctaac tgggagcggt cagcggatac tggcgagctt
      121 gaggggggca aaggaagatg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gtcttctggg cccatcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01014818             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14989 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014818
VERSION     KHUT01014818.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14989"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           75 a           49 c           79 g           50 t
ORIGIN      
        1 tacgaatggg gcaagcgttg ttcggaatca ctgggcttaa agcgtatgca ggtggccaag
       61 caagtcagaa gtgaaatccc tgggctcaac ccaggaattg cttttgaaac tgctaggcta
      121 gaatcccgga gaggttaaca gaattccgag tgtagaggtg aaattcgcag atattcggag
      181 gaataccagt ggcgcaagcg gttaactgga cgggcattga cactcagata cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014819             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14990 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014819
VERSION     KHUT01014819.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14990"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           59 c           99 g           40 t
ORIGIN      
        1 gacggagggg gcaagcgttg tccggactca ctgggcgtaa agggtgcgta ggcggttgtg
       61 tgcgtcggcg ctgaaatctc gaggcttaac ctcgaggcgc gtgccgatac gacacgactt
      121 gaggctctga gagggtggcg gaactcctgg tgtagtggtg aaatgcgtag agatcaggaa
      181 gaacacccgt ggcgcaagcg gccacctggc agagacctga cgctgaggca cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014820             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14991 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014820
VERSION     KHUT01014820.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14991"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           50 c           97 g           47 t
ORIGIN      
        1 tacggggggg ggcaagcgtt gttcggaatt actgggcgta aagggctcgt aggtggccaa
       61 ctaagtcgga cgtgaaatcc ctcggcttaa ccggggaact gcgtccgata ctggttggct
      121 tgagtttggg agagggatgc ggaattccag gtgtagcggt gaaatgcgta gatatctgga
      181 ggaacaccgg tggcgaaggc ggcatcctgg accaatactg acgctgagga gcgaaagcca
      241 ggggagcaaa cggg
//
LOCUS       KHUT01014821             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14992 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014821
VERSION     KHUT01014821.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14992"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           81 g           56 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatta ttgggcgtaa agggtgtgta ggcggttttg
       61 ttagtcttat gttaaagctc ccggcttaac cgggaaaatg catgagatac ggcaaaacta
      121 gaggatgcga gaggtatgcg gaactcatag tgtaggggtg aaatccgttg atattatggg
      181 gaacaccaaa agcgaaggca gcatactggc gcactcctga cgttgagaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014822             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14993 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014822
VERSION     KHUT01014822.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14993"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggattca ctgggcttaa agggcgcgta ggcggctgac
       61 caagtcgggg gtgaaatcgt tcggcttaac cggacaaggg cttccgatac tggtcagctg
      121 gagggagata ggggtgcgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgcactgga tctctactga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014823             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14994 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014823
VERSION     KHUT01014823.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14994"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           40 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgtgta ggtggtgacg
       61 tgagtcaggt gtgaaagtcc cgggcttaac ctggggatgg cgcctgatac tgcgtggcta
      121 gagtattgta gaggaaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacatcagt ggcgaaggcg actttctgga caaatactga cactgagaca cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014824             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14995 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014824
VERSION     KHUT01014824.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14995"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           47 c           91 g           52 t
ORIGIN      
        1 tacgtaggtg acaaatgtta tccggaatta ctgggcgtaa agggtctgta ggcggctgtt
       61 taggtctgat gtgacacctc ccggcttaac tgggagaatg cattggaaac cgggcagctg
      121 gaatcacgtc gaggtcggtg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacctcgaa agcgaaggca gccggctaga cgatgattga cgctgaggga cgatagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014825             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14996 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014825
VERSION     KHUT01014825.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14996"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           53 c           95 g           43 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggccttt
       61 caggtccgtt gtgaaaaccc gaggctcaac ctcgggacgt cgatggaaac cgaaaggcta
      121 gagtccggaa gaggagagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gctctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014826             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14997 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014826
VERSION     KHUT01014826.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14997"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           54 c           92 g           43 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggcgatg
       61 caagacaggt gtgaaatccc cgggctcaac ctgggaactg cacttgtgac tgcatggcta
      121 gagtgcgtca gagggaggtg gaattccgcg tgtagcagtg aaatgcgtag agatgcggag
      181 gaacaccgat ggcgaaggca gcctcctggg atgacactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014827             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU14999 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014827
VERSION     KHUT01014827.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU14999"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           88 g           49 t
ORIGIN      
        1 gacgaaggtg gcgagcgtta ctcggaatta ctaggcgtaa agcgtgggta ggtggctcag
       61 caagtctgtt gtgaaagctc ctggcttaac tgggagaggc caatggatac tgctgggctt
      121 gaatcgtgga gaggtcactg gaattcctgg tgtagcggtg aaatgcgtag agatcaggag
      181 gaacaccaat ggcgaaagca ggtgactgga cacgtattga cactcagcca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014828             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15000 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014828
VERSION     KHUT01014828.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15000"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           86 g           53 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattca ctgggcgtaa agggtgcgta ggtggttggg
       61 taagtctgat gtgaaatctc tgggctcaac ccagaaatgg cattggatac tatccggcta
      121 gaggagtgga gggggaactg gaatacttgg tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agttcctgga cacttcctga cactgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014829             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15001 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014829
VERSION     KHUT01014829.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15001"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           49 c          102 g           48 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccgaagtta ctgggcgtaa agcgcgcgta ggcggtcctt
       61 taagtctggg gtgaaaggtt cagcgctcaa cgcgaacagt gccttggata ctgggggact
      121 tgagtctggg agagggcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccgg tggcgaaggc ggctgcctgg cctaagactg acgctgaggt gcgaaagcgt
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014830             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15002 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014830
VERSION     KHUT01014830.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15002"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           43 c           76 g           63 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattta ctgggtttaa agggtgcgta ggtgggtaga
       61 taagtcagcg gtgaaatctc caggcttaac ttggaaactg ccattgatac tatctgtctt
      121 gaatattctg gaggtgagcg gaatatgtca tgtagcggtg aaatgcttag atatgacata
      181 gaacacccat tgcgaaggca gctcactacg ggattattga cactgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014831             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15003 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014831
VERSION     KHUT01014831.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15003"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           58 c          102 g           40 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggccac
       61 caagtctggg gtgaaagccc ccggctcaac cggggaattg cctgggatac tggtggtctg
      121 gagggaggta ggggcagcgg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggt ggcgaaagcg cgctgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014832             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15004 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014832
VERSION     KHUT01014832.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15004"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           33 c           70 g           71 t
ORIGIN      
        1 tacgaaggga gtaagcgtta tccgaaatga ttgggcgtaa agggttcgta ggtggtaatt
       61 gttgtcatat gtgaaatctt gaaatttcct ttcaaatatg cgtatgatac tcttttactt
      121 gagtatatca ggggataaca gaattttgta agtaggagta aaatccattg atctgcaaag
      181 gaatgccagt ggcgaaagcg gttatctggg atatatactg acgctgagga acgaaagcat
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014833             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15005 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014833
VERSION     KHUT01014833.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15005"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           53 c           94 g           47 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagctcgta ggcggctcag
       61 caagtcgact gtgaaaactc cgggctcaac tcggagacgc cagtcgatac tgctgtggct
      121 agagtccgac aggggagcat ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc ggtgctctgg gtcggtactg acgctgagga gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01014834             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15006 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014834
VERSION     KHUT01014834.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15006"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           56 c           88 g           45 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtcgcg
       61 caagacagat gtgaaatccc cgggcttaac ctgagaatcg catttgtgac tgcacggctt
      121 gagtgcggca gagggggatg gaattccgcg tgtagcagtg aaatgcgtag atatgcggag
      181 gaacaccgat ggcgaaggca atcccctggg cctgcactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014835             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15007 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014835
VERSION     KHUT01014835.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15007"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           49 c           95 g           51 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagctcgta ggcggttcga
       61 taagccggat gtgaaatctc cacgctcaac gtggagggtg cattcggtac tgtcgtgact
      121 agagtcaggt agaggagtgt ggaattcctg gtgtagcggt gaaatgcgca gagatcagga
      181 ggaacacccg tagcgaaggc ggcactctgg gccttgactg acgctgagga gcgaaagcgt
      241 gggtagcgaa cagg
//
LOCUS       KHUT01014836             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15008 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014836
VERSION     KHUT01014836.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15008"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           56 c          100 g           42 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttgt
       61 caagtcgggg gtgaaatcgt tcggcccaac cggacaaggg cccccgatac tggcagtctt
      121 gagggaggta ggggtcggtg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gccgactgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014837             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15009 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014837
VERSION     KHUT01014837.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15009"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           93 g           52 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ctgggtgtaa agggcgtgta ggcgggaatg
       61 caagtcagat gtgaaattcc agggctcaac cctggcgctg catctgaaac tgtatttctt
      121 gagtgtcgga gaggaaagtg gaattcccag tgtagcggtg aaatgcgtag agattgggag
      181 gaacaccagt ggcgaaggcg gctttctgga cgataactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014838             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15010 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014838
VERSION     KHUT01014838.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15010"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           45 c           90 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggattta ctgggcgtaa agggcgcgta ggtggttaga
       61 taagtgagat gtgaaatccc cgggctcaac ctgggaactg catcttatac tgtctagctg
      121 gagtacatga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc atgatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014839             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15011 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014839
VERSION     KHUT01014839.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15011"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           49 c           92 g           52 t
ORIGIN      
        1 tacgggggga gcaagcgttg tccggattta ttgggtgtaa agggcgtgta ggcggtccga
       61 taagtcaggg gtgaaacctg cgagctcaac tcgcagcctg cctttgatac tgttggactt
      121 gagtctggta gagggtcatg gaattccggg tgtagcggtg aaatgcgtag atatccggaa
      181 gaacaccaat tgcgaaggca gtgacctggg ccagtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014840             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15012 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014840
VERSION     KHUT01014840.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15012"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcgtgta ggtggcggta
       61 taagtcgaat gtgaaatccc tcagctcaac tgaggaagtg catccgatac tgtatcgctt
      121 gagtaccgga ggggatggcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacctgt ggcgaaggcg gccatctgga cggtaactga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014841             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15013 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014841
VERSION     KHUT01014841.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15013"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           61 c          100 g           39 t
ORIGIN      
        1 gacgtactgc gcgaacgtta ttcggaatca ctgggcttaa agggtgcgca ggcggcttcc
       61 aaagcccgaa gtgaaaggtc ccggcccaac cggggacgtg cttcggggac ttggaggctc
      121 gggcgagcta ggggtccgtg gaactcccgg tggagcggtg aaatgtgttg agatcgggag
      181 gaacgccggt ggcgaaagcg acggactggg gctttgccga cgctcatgca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014842             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15014 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014842
VERSION     KHUT01014842.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15014"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           55 c           99 g           45 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggactta ctgggcgtaa agcgtgtgca ggcgggtggc
       61 tatgtgccgt gtgaaagctc cgcgcttatc gcggagaggt cgcggtagac aggctacctg
      121 gaggctcgga gaggtcagtg gaattctcgg tgtagtggtg aaatgcgtag atatcgagag
      181 gaacaccagt ggcgcaagcg gctgactggc cgagacctga cgctgagaca cgacggcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01014843             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15015 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014843
VERSION     KHUT01014843.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15015"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           47 c           87 g           53 t
ORIGIN      
        1 gacgagggat cctagcgttg ttcggaatta ttgggcgtaa agcgggtgca ggtggctttg
       61 taagtcaggt gtgaaatccc agggctcaac cctggaagtg catttgatac tgcgaagctt
      121 gagtgtggga gaggtgagta gaattccagg tgtagtggtg aaatacgtag atatctggag
      181 gaataccgga ggcgaaggcg gctcactggc ccaacactga cactcagacc cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014844             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15016 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014844
VERSION     KHUT01014844.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15016"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           87 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgttg ttcggaatta ctgggcgtaa agcgcacgta ggcgggttcg
       61 tatgtcgatt gtgaaagccc taggcttaac ctgggaattg cagtcgaaac tgcgagtctt
      121 gaatgttcaa gaggatggcg gaattcctgg tgtagtagtg aaatacgtag atatcaggaa
      181 gaacacccga ggcgaaggcg gctatctggt gaaacattga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014845             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15017 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014845
VERSION     KHUT01014845.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15017"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           47 c           82 g           53 t
ORIGIN      
        1 gacagaggtg ccaagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggtggatttg
       61 taagtacctt gtgaaatccc acagctcaac tgtggaactg ctcggtatac tgcgaatctt
      121 gagctactta ggggcaaccg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca ggttgctggg agtatgctga cactgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014846             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15018 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014846
VERSION     KHUT01014846.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15018"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           93 g           48 t
ORIGIN      
        1 tacggagggt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggcttga
       61 taagtcgggt gtgaaatccc tgggctcaac ctgggaactg catccgatac tgtcaagcta
      121 gagtgtggga gagggtgacg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg gttgcctggc ccaacactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014847             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15019 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014847
VERSION     KHUT01014847.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15019"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           48 c           88 g           49 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatta ttgggcgtaa agcgtgcgca ggcggttttg
       61 taagacagat gtgaaatccc cgggcttaac ctgggaactg catttgtgac tgcaaggcta
      121 gagtgtgtca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg acaacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014848             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15020 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014848
VERSION     KHUT01014848.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15020"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           46 c           83 g           54 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatta ctgggcgtaa agcgtacgta ggcgggagtt
       61 taagtgcaat gcgaaatctt gtgactcaat catatagact gtattgcata ctgggcacct
      121 agaggacggc agaggcaagt ggaattggta gtgtagcagt gaaatgcgta gatattacca
      181 ggaacaccaa tggcgaaggc agcttgctgg gccgtatctg acgctgaggt acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01014849             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15021 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014849
VERSION     KHUT01014849.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15021"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           98 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcggaaggg
       61 cacgtccgga gctgaaatcc cccggctcaa ccggggaagt ggcgcggata cgactcttct
      121 ggaggggcgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgcttactg acgctgagac gcgaaagcca
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014850             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15022 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014850
VERSION     KHUT01014850.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15022"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           50 c           98 g           50 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agagctcgta ggcggtgtgt
       61 cacgtcgttc gtgaaaacct gcagcttaac tgtgggcgtg cggtcgatac gggcatcact
      121 ggagttcggc aggggagact ggaattcctg gtgtagcggt gaaatgcgca gatatcagga
      181 ggaacaccgg tggcgaaggc gggtctctgg gccgtaactg acgctgagga gcgaaagcat
      241 ggggagcgaa cagg
//
LOCUS       KHUT01014851             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15023 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014851
VERSION     KHUT01014851.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15023"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           57 c          103 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcacga
       61 taagcgtgcg gtgaaagccc ggggctcaac cccgggtcgg ccgtgcgaac tgtcgagctc
      121 gagcactgta gaggcagatg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gtctgctggg cagtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014852             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15024 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014852
VERSION     KHUT01014852.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15024"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           95 g           40 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacgcc
       61 tcagtgcctt gtgaaatccc acggctcaac cgtggaactg ctgggcatac tgggcgtctt
      121 gggccactta ggggtcgtcg gaacaggcgg tggagcggtg aaatgcgtag atatcgcctg
      181 gaacgccaac ggcgaaagca gacgactggg agtgtgccga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014853             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15025 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014853
VERSION     KHUT01014853.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15025"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           42 c           85 g           54 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ttcggaatta ctgggcgtaa agggttcgta ggcgggaaga
       61 taagttaagt gtgaaatccc taggctcaac ctaggacgtg catttaaaac tgtttttctt
      121 gagtgttgga gagggtagtg gaattgctgg tgtaggagtg acatccgtag agatcagcag
      181 gaacatcaga ggcgaaagcg actgcctggc caaacactga cgctgaggaa cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014854             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15026 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014854
VERSION     KHUT01014854.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15026"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           92 g           44 t
ORIGIN      
        1 tacagggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggcaact
       61 taagtcggat gtgaaagccc ttggctcaac cgaggaagtg cacccgaaac tgagtcgctg
      121 gagtacgaaa gaggatcgcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gcgatctggg tcgatactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014855             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15027 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014855
VERSION     KHUT01014855.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15027"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           61 c          100 g           36 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggtgcgta ggcgggcgcg
       61 tcagtcaggg gtgaaatccc gcggcccaac cgcggaaggg cctctgatac tgcgtgccta
      121 gaggggggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcacgctggg tccccactga cgctgaggca cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014856             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15028 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014856
VERSION     KHUT01014856.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15028"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           56 c           99 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agagcgtgta ggcggtccgg
       61 taagtctgcc gtgaaaaccc ggggctcaac cccgggcgtg cggtggaaac tgccgggcta
      121 gaggatggta gaggcgagcg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccagt agcgaaggcg gctcgctggg ccattcctga cgctgagacg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014857             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15029 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014857
VERSION     KHUT01014857.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15029"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           53 c          100 g           41 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggaatta ctgggcgtaa agcgcgcgca ggcggcaccc
       61 aaagtactca gtgaaatctc cctgctcaac ggggagggtg gtgagtagac ttgggagcta
      121 gagagcggta gaggggggtg gaattgctgg tgtagcggtg aaatgcgtag agatcagcag
      181 gaagacccgt ggcgaaggcg gccccctggg ccgtatctga cgctgaggcg cgaaggctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014858             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15030 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014858
VERSION     KHUT01014858.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15030"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           58 c          107 g           38 t
ORIGIN      
        1 tacggagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggctcac
       61 gaaagcgtgc ggtgaaagcc cggggctcaa ccccgggtcg gccgtgcgga ctgcggggct
      121 cgagcactgt aggggcagat ggaattccgg gtgtagcggt ggaatgcgta gagatccgga
      181 agaacaccgg tggcgaaggc ggtctgctgg gcagttgctg acactgaggc gcgacagcgt
      241 ggggagcaaa cagg
//
LOCUS       KHUT01014859             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15031 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014859
VERSION     KHUT01014859.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15031"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           44 c           91 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttgat
       61 taagttgttt gtgaaatccc cgggcttaac ctgggaactg caagcaaaac tggtcagctt
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014860             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15032 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014860
VERSION     KHUT01014860.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15032"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           47 a           63 c          111 g           32 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatca ttgggcgtaa agcgcgcgca ggcggcgctg
       61 agagcccggc gtgaaagccc ccggctcaac cgggggaggc cgtcggggac ctcagggctg
      121 gaggccggca ggggtgagcg gaattcccgg tgtagtggtg gaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctcactggg ccggacctga cgctgaggcg cgaaagcgtg
      241 gggagcgatc cgg
//
LOCUS       KHUT01014861             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15033 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014861
VERSION     KHUT01014861.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15033"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           41 c           91 g           53 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgcgta ggtggttaat
       61 taagttagct gtgaaatctc tgggcttaac ctggggcgga cggctaatac tgaataactt
      121 gagtatagga gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctggc ctaatactga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014862             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15034 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014862
VERSION     KHUT01014862.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15034"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           94 g           45 t
ORIGIN      
        1 gacgaaggtg gcgaccgttg ttcggaatca ctgggcataa agcgcacgta ggcggtgagg
       61 taagtcaggt gtgaaatccc tcggctcaac cgaggaactg cgcttgaaac taccttgctt
      121 gaggacagga ggggagcgtg gaattcctgg tggagcggtg aaatgcgttg atatcaggag
      181 gaacaccggc ggcgaaggcg acgctctggc ctgtttctga cgctgaggtg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014863             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15035 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014863
VERSION     KHUT01014863.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15035"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           51 c          100 g           45 t
ORIGIN      
        1 tacgtaggag gcaagcgttg tccggattta ctgggcgtaa agagcgtgta ggcggcatgg
       61 taagtctgct gtgaaagctc ctcgcttaac gaggagaggt cggtggaaac tgccaggcta
      121 gaggacggta gaggggggtg gaattcccgg tgtagtggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gccccctggg ccgctcctga cgctgagacg cgaaggctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014864             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15036 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014864
VERSION     KHUT01014864.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15036"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           52 c           94 g           44 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcggcttcg
       61 caagtcgggt gtgaaatccc ccagcttaac tggggaagtg cgctcgaaac tgcggagctc
      121 gagtaccgga gaggatggcg gaatgcccca agtagaggtg aaattcgtag atatggggcg
      181 gaacaccggt ggcgaaggcg gccatctgga cggatactga cgctgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014865             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15037 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014865
VERSION     KHUT01014865.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15037"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           91 g           47 t
ORIGIN      
        1 tacggagggt gcaaacgttg ctcggaatca ttgggcgtaa agcgcacgta ggcggtctcg
       61 taagtcggtt gtgaaatccc tcggcttaac caaggaagtg cattcgaaac tgcgagactt
      121 gagtacggga gagggtcgcg gaattctcgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccagt ggcgaaggcg gcgacctgga ccgatactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014866             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15038 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014866
VERSION     KHUT01014866.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15038"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           48 c           98 g           51 t
ORIGIN      
        1 tacgtagggt gcgagcgttg tccggaatta ttgggcgtaa agggctcgta ggcggtttgt
       61 cgcgtcggga gtgaaaacgc gctgcttaac agcgcgcttg ctttcgatac gggcagactg
      121 gaggtatgca ggggagaacg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccggt ggcgaaggcg gttctctggg cattacctga cgctgaggag cgaaagtgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014867             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15039 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014867
VERSION     KHUT01014867.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15039"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           87 g           52 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatca ctgggcgtaa agcgcgcgta ggcggctttt
       61 caagtcgggg gtgaaatcct gaggcttaac ctcagaattg ccttcgaaac tgtaaagcta
      121 gagtgtcgga ggggatagcg gaattcctaa tgtagaggtg aaattcgtag atattaggag
      181 gaacaccggt ggcgaaggcg gctatctgga cgacaactga cgctgaggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014868             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15040 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014868
VERSION     KHUT01014868.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15040"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           88 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggactta ctgggcgtaa agggtgcgta ggtggaagga
       61 taagtcagat gtgaaaggcc tgggcttaac ctaggaactg caattgaaac tgtccatcta
      121 gagttgagta gaggggagcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacacctgt tgcgaaggcg gctctctgga ctcaaactga cgctgaggca cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014869             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15041 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014869
VERSION     KHUT01014869.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15041"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           46 c           90 g           49 t
ORIGIN      
        1 tacggagggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctagg gtgaaagcct tgagcttaac tcaagaagtg ccctagaaac ggtgagactc
      121 gagtcctgga gaggcaagtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcttgctgga caggtactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014870             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15042 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014870
VERSION     KHUT01014870.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15042"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           49 c           97 g           45 t
ORIGIN      
        1 tacagagggt gcgagcgttg ttcggaatta ttgggcgtaa agcgcgtgta ggcgggattg
       61 tgtgtcaggt gtgaaagccc tcggctcaac cgaggaagtg cgcctgaaac tgcagttctt
      121 gagtgtcgga gagggtggcg gaattcccgg tggagaggtg aaattcgtag atatcgggag
      181 gaacaccaga ggcgaaggcg gccacctgga cgaacactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014871             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15043 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014871
VERSION     KHUT01014871.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15043"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           53 c           76 g           50 t
ORIGIN      
        1 tacagagacc ccgagcgtta tccggaatta ctgggcgtaa agcgcgagca gctggtcgtg
       61 ttagtctgat gtaaaatcat ggggcctaac tccatgtccg catcggaaac ggcacggctt
      121 gagcagatga gaggagagca gaacatatgg tgtaggggtg aaatccactg atatcatatg
      181 gaataccaaa agcgaaggca gctctctagc gtcttgctga cagtcaatcg cgaaagcgtg
      241 gggatcaaaa agg
//
LOCUS       KHUT01014872             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15044 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014872
VERSION     KHUT01014872.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15044"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           42 c           79 g           52 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggttagg
       61 caagttaaca gtgaaaacga ggctcaacct cgtggaagtt gttaagactg cttggctaga
      121 gaaccggaga ggttaacgga actcttatgg aggggtaaaa tccaacgata tgagcgggaa
      181 caccaataac ggcgaaagca gttaactatt cggtttctga cattgaggca cgaaagcgtg
      241 gggagcaaaa cgg
//
LOCUS       KHUT01014873             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15045 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014873
VERSION     KHUT01014873.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15045"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           41 c           91 g           52 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggtggttaaa
       61 taagttggat gtgaaatccc tgggcttaac ctaggaatgg cattcaaaac tgtttgactg
      121 gagtactgta gagggtagtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacaccagt ggcgaaggcg gctacctgga cagatactga cactgaggcg cgagagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014874             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15046 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014874
VERSION     KHUT01014874.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15046"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           50 c           99 g           45 t
ORIGIN      
        1 tacggagggt gcgagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggcggtggcg
       61 tgagtcggat gtgaaagccc cgggctcaac ctgggaatgg cattcgatac cgcgttgcta
      121 gagtttggga gagggaaggg gaattccggg tgtagcggtg aaatgcgtag atatccggag
      181 gaacaccagt ggcgaaggcg ccttcctggc ctaagactga cgctgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014875             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15047 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014875
VERSION     KHUT01014875.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15047"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           60 c           95 g           40 t
ORIGIN      
        1 gacgaaccga gcgaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcgggccat
       61 caagtcaggg gtgaaatccc acggctcaac cgtggcagtg cctttgatac tggtggcctc
      121 gagcgaggta ggggcgtgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacaccggt ggcgaaagcg gcgcgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014876             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15048 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014876
VERSION     KHUT01014876.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15048"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           55 c           96 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggcaggt
       61 aaagtccggg gtgaaatctt tcagcttaac tggaaaagtg ccccggatac tgacttgctc
      121 gagggaggca ggggccgagg gaacttccgg tggagcggtg aaatgcgtag agatcggaag
      181 gaacgccggt ggcgaaagcg ctcggctgga cctcttctga cgctgaggcg cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014877             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15049 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014877
VERSION     KHUT01014877.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15049"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           52 c           98 g           44 t
ORIGIN      
        1 tacggagggt gcaagcgtta cccggaatca ctgggcgtaa agggcgtgta ggcggaaggt
       61 taagtccgac tttaaagacc ggggctcaac cccgggcctg ggttggagac tggctttctg
      121 gacctctgga gaggcaactg gaattcctgg tgtagcggtg gaatgcgtag ataccaggag
      181 gaacaccgat ggcgaaggca ggttgctgga cagaaggtga cgctgaggcg cgaaagtgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014878             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15050 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014878
VERSION     KHUT01014878.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15050"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           40 c           85 g           59 t
ORIGIN      
        1 tacgaggggt gcaaacgtta ttcggattta ctgggtgtaa agggtgtgta gacggtgcgt
       61 taagtttgct gttaaaactt ttggcctaac caaaagcttg cggtgaaaac tggcgtgcta
      121 gagagtggaa gagagaagtg gaattctcgg agtagcggta aaatgcgtag atctcgagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccatttctga cgttgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014879             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15051 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014879
VERSION     KHUT01014879.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15051"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           47 c           83 g           56 t
ORIGIN      
        1 tacgagtgcc ccgagcgtta tccggaatta ttgggcgtaa agggtgtgta ggtggtcttg
       61 ttagtcttct gttaaatctc ccggcttaac cgggaacatg caggggaaac ggcaagacta
      121 gaggatgtga gaggtttgcg gaactcatgg tgtaggggtg aaatccgttg atatcatggg
      181 gaacaccaaa tgcgaaggca gcaaactggc gcattcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014880             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15052 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014880
VERSION     KHUT01014880.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15052"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           50 c           91 g           45 t
ORIGIN      
        1 gacagagggt gcaaacgttg ttcggaatta ctgggcgtaa agcgtgtgta ggcggtcctg
       61 aaagtcggat gtgaaagccc cgggctcaac ccgggaagtg cactcgatac tccaggactt
      121 gagtgtcgga gaggttggtg gaattctcgg tgtagaggtg aaattcgtag atatcgagag
      181 gaacaccggt ggcgaaggcg gccaactgga cgaacactga cgctgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014881             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15053 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014881
VERSION     KHUT01014881.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15053"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           94 g           50 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggcggttagt
       61 tgcgtgtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcaaac gggctaacta
      121 gagtatgaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag atatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttaatactga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014882             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15054 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014882
VERSION     KHUT01014882.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15054"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agcgtgcgta ggtgggcgtt
       61 taagtcgaat gtgaaagccc tgggcttaac ctgggaactg catccgatac tggacgtcta
      121 gagtatggta gaggaaagtg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacatcagt ggcgaaggcg actttctgga tcaatactga cactgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014883             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15055 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014883
VERSION     KHUT01014883.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15055"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           51 c           85 g           48 t
ORIGIN      
        1 tacgaagggg gcgagcgttg ttcggaatca ctgggcgtaa agcgtgcgta ggctgtcttg
       61 caagttggaa gtgaaagccc ggggctcaac cccggaattg ctttcaaaac tgcaagactc
      121 gaattcggga gaggatagcg gaattcccag tgtagtagtg aaatacgtag atattgggag
      181 gaacaccagt ggcgcaagcg gctatctgga ccgacattga cgctgaggca cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014884             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15056 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014884
VERSION     KHUT01014884.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15056"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           90 g           50 t
ORIGIN      
        1 tacagagggt gcaagcgttg ctcggaatca ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtcaggg gtgaaatcct tgggcttaac tcaagaagtg cccttgaaac ggtgagactg
      121 gagtcttgga gaggttcgcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgagctgga caagtactga cactcaacta cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014885             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15057 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014885
VERSION     KHUT01014885.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15057"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           56 a           52 c          105 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ctgggcgtaa agggcgcgca ggcggtttgc
       61 taagtccgct gtgaaagacc ggggctcaac cccggggtgt cagcggagac tggtgaactg
      121 gagtgctgga gagggatgcg gaatacctgg tgtagcggtg gaatgcgtag agatcaggtg
      181 gaacaccgat ggcgaaggca gcatcctggc cagagactga cgctgaggcg cgaaagctgg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014886             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15058 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014886
VERSION     KHUT01014886.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15058"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggattta ctgggcgtaa agggcgcgta ggcggatttc
       61 taagtcgggg gtgaaagccc agggctcaac cctggaattg ccttcgatac tggaagtctt
      121 gagtacggga gaggtgagtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg actcactggc ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014887             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15059 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014887
VERSION     KHUT01014887.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15059"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           45 c           96 g           49 t
ORIGIN      
        1 tacgtaggtg gcgagcgttg tccggaatta ttgggcgtaa agggtgcgta ggcggtccgt
       61 taagtctagt ttgaaagacc acggcttaac cgtgggaggg gattagaaac tggcggactt
      121 gagggtaaga gaggaaggtg gaattccacg tgtagcggtg aaatgcgtag agatgtggag
      181 gaacaccagt ggcgaaggcg gccttctggc ttacccctga cgctgaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014888             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15060 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014888
VERSION     KHUT01014888.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15060"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtaggat gcaagcgttg tccggattta ttgggcgtaa agcgtccgta ggcggtgatt
       61 taagttcgat gttaaagact ggagctcaac tccaacatgg cattgaatac tggatcgctg
      121 gagtgcggca gaggtaaagg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacaccagt ggcgaaagcg ctttactggg ccgtaactga cgctgaggga cgaaagccag
      241 ggtagcgaaa ggg
//
LOCUS       KHUT01014889             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15061 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014889
VERSION     KHUT01014889.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15061"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           88 g           49 t
ORIGIN      
        1 tacgaagggg gctagcgttg ctcggaatta ctgggcgtaa agggcgcgta ggcgggtgtt
       61 taagtcagag gttaaatccc agagctcaac tctggaactg cctttgatac tggacaccta
      121 gagtacggga gaggtgtgtg gaactccgag tgtagaggtg aaattcgtag atattcggaa
      181 gaacaccagt ggcgaaggcg acacactggc ccgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014890             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15062 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014890
VERSION     KHUT01014890.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15062"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           56 c           97 g           40 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcctcg
       61 taagtccgct ctgaaagcct ggggctcaac cccaggaggc gggtggatac tgcgaggctc
      121 gagtacggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014891             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15063 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014891
VERSION     KHUT01014891.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15063"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           54 c           96 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcctga
       61 taagtccgtt ctgaaagtcc ggggctcaac cccgggaggc ggatggatac tgtcaggctc
      121 gagtccggaa gaggcaagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gcttgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014892             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15064 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014892
VERSION     KHUT01014892.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15064"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           58 c           97 g           38 t
ORIGIN      
        1 tacgtagggt gcgagcgtta atcggaatta ctgggcgtaa agcgtgcgca ggcggtccgc
       61 taagcccgct gtgaaagccc cgggctcaac ctgggaacgg cagtggggac tgacgggctg
      121 gagtgcggca gagggaggtg gaattccacg tgtagcagtg aaatgcgtag agatgtggag
      181 gaacaccgat ggcgaaggca gcctcctggg ccgcaactga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014893             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15065 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014893
VERSION     KHUT01014893.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15065"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           91 g           49 t
ORIGIN      
        1 tacagagggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcatgta ggcggcttgt
       61 taagtcgatt gtgaaatccc acggctcaac cgtggaagtg ctgtcgaaac tggcgagctg
      121 gagtcctgac gagggtggcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaggcg gccacctggg aaggtactga cgctcagatg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014894             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15066 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014894
VERSION     KHUT01014894.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15066"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           52 a           60 c          102 g           39 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggattca ctgggcttaa agggcgcgta ggcgggcgtt
       61 cttgtccggg gtgaaagccc acggcttaac cgtggaagtg cctcggaaac gggacgcctc
      121 gaggggggta ggggcctgcg gaacttccgg tggagcggtg aaatgcgtag atatcggaag
      181 gaacgccggt ggcgaaagcg gcgggctgga ccccttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014895             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15067 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014895
VERSION     KHUT01014895.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15067"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           46 c           99 g           53 t
ORIGIN      
        1 gacgtaggga gcaagcgttg tccggattta ttgggcgtaa agggctcgta ggcggcttgt
       61 cacgtctgtc gtgaaagatc agggcttaac cctggttctg cggtggatac gggcaggctt
      121 gaggcaagta ggggagatcg gaattccatg tgtagcggtg aaatgcgcag atatgtggag
      181 gaacaccggt ggcgaaggcg gatctctggg cttgttctga cgctgaggag cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014896             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15068 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014896
VERSION     KHUT01014896.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15068"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           61 c           99 g           36 t
ORIGIN      
        1 gacagaggag ccaagcgttg tccggattga ctgggcgtaa agcgcacgca ggcggccgtg
       61 cacgtggggt gtgaaagctg acggcttaac tgtcagaggc catcccatac ggcgcggctc
      121 gagccctgca gaggtccacg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacacccag ggggaaacca gtggactggg caggggctga cgctcaggtg cgaaagcgtg
      241 gggagcgaac cgg
//
LOCUS       KHUT01014897             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15069 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014897
VERSION     KHUT01014897.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15069"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           87 g           52 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ctgggcgtaa agagcgtgta ggcggtctag
       61 aaagtcagat gtgaaatccc ggggcttaac cccggacgtg catttgaaac tcctaggcta
      121 gagtgtggta gaggatagtg gaatacctag tgtagtggtg aaatacgtag atattaggta
      181 gaacaccggt ggcgaaggcg gctatctggg ccataactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014898             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15070 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014898
VERSION     KHUT01014898.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15070"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           86 g           53 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggttgtg
       61 ttagtctcat gtcaaattct tcggcttaac cggggtaccg catgggatac ggcacaacta
      121 gaggatgcga ggggtctgtg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca gcaggctggc gcactcctga cactgaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014899             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15071 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014899
VERSION     KHUT01014899.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15071"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           50 c           88 g           50 t
ORIGIN      
        1 tacgtagggt ccaagcgtta ttcggaataa ctgggcgtaa agggcgtgta ggtggctcga
       61 gaagtcttgc gttaaatcaa tcggctcaac cgattggcgg cgtgggaaac catcgagctt
      121 gagtcttgca ggggctagcg gaattcccgg tgtagcagtg aaatgcgttg atatcgggaa
      181 gaacaccaat ggcgaaggca gctagctagg caagttctga cactgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014900             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15072 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014900
VERSION     KHUT01014900.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15072"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacgaaggtg gcaagcgttg ctcggaatca ctgggcataa agcgcgcgta ggtggcctga
       61 taagtcggtg gtgaaatccc atggctcaac catggaactg ccgtcgatac tatcgggctt
      121 gaggacggta ggggagagtg gaactcctgg tgtagcggtg aaatgcgtag atatcaggag
      181 gaacgccggt ggcgaaagcg actctctggg ccgctcctga cactgatgtg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014901             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15073 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014901
VERSION     KHUT01014901.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15073"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           91 g           46 t
ORIGIN      
        1 tacgtagggt gcaagcgtta atcggaatga ctgggcgtaa agggtgcgca ggcggctgat
       61 taagtcagat gtgaaatacc tgggctcaac ctaggaggtg catttgagac tgatgagcta
      121 gagtgtgtaa gaggggggta gaattccacg agtagcagtg aaatgcgtag agatgtggag
      181 gaataccgat ggcgaaggca gccccctggg ataacactga cgctcaggca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014902             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15074 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014902
VERSION     KHUT01014902.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15074"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           63 a           51 c           93 g           46 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggattta ttgggcgtaa agagcgtgta ggcggcctag
       61 taggtcagct gtgaaaactc gaggctcaac ctcgagctgt cggttgaaac cactaggcta
      121 gagtccggaa gaggagaatg gaattcccgg tgtagcggtg aaatgcgcag atatcgggaa
      181 gaacacccgt ggcgaaggcg gttctctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014903             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15075 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014903
VERSION     KHUT01014903.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15075"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c          100 g           44 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg ttcggaatta ttgggcgtaa agggcgcgta ggcggtcgct
       61 taagtcagcc gtgaaagccc ggggctcaac cccggagggt cggctgatac tgggtggcta
      121 gagtacggaa gaggtagctg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctga ggcgaaggcg ggctactggg ccgatactga cgctgatgcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014904             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15076 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014904
VERSION     KHUT01014904.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15076"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           54 c           97 g           48 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggttcc
       61 tcagtctgtg gtgaaatcct ccagcttaac tggagaagtg ccatggatac tgggagtctc
      121 gaggagggta ggggcatctg gaacagccgg tggagcggtg aaatgcgttg atatcggctg
      181 gaactccggt ggcgaaggcg aggtgctgga ccctttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014905             256 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15077 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014905
VERSION     KHUT01014905.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15077"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>256
                     /product="16S ribosomal RNA"
BASE COUNT           80 a           41 c           82 g           53 t
ORIGIN      
        1 gacataggtg gcaaacgtta tccggaatta ttgggcgtaa agggtgcgta gatggtatga
       61 taagttagcg gtgggaaatc ggggctcaac cccgtggaag ctgataatac tatcgtgcta
      121 gagtgtagga gaggttaacg gaactccccg tggagcggtg aaatgcatag atatggggaa
      181 gaacaccaat aatagcgaag gcagttaact attctactac tgacattgag gcacgaaagc
      241 gtggggagca aaaagg
//
LOCUS       KHUT01014906             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15078 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014906
VERSION     KHUT01014906.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15078"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           41 c           91 g           54 t
ORIGIN      
        1 gacggagggg gctagcgttg ttcggaatta ctgggcgtaa agagcgcgta ggcggattag
       61 taagttggga gtgaaagccc ggggcttaac ctcggaatag ctttcaaaac tactagtctt
      121 gagtgtagta ggggatgatg gaattcctag tgtagaggtg aaattcttag atattaggag
      181 gaacaccggt ggcgaaggcg gtcatctggg ctacaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014907             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15079 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014907
VERSION     KHUT01014907.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15079"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           54 c           87 g           46 t
ORIGIN      
        1 tacgtagacc acaagcgtta tccggattta ttgggcgtaa agcgcgtgta gggggtccaa
       61 cgcatctctt gttaaatccc ggggcctaac cccggaaatg caagggagat ggtcggactg
      121 gaggaggtta gaggcaagtg gaacgcacgg tgtaggggtg aaatccgttg atatcgtgcg
      181 gaacaccaaa ggcgaaggca gcttgctggg accttcctga ccctgagacg cgaaagcgtg
      241 gggagcaaaa agg
//
LOCUS       KHUT01014908             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15080 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014908
VERSION     KHUT01014908.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15080"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           42 c           89 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcggaatta ctgggcgtaa agagcgcgta ggcggtttga
       61 taagtctgat gtgaaagccc tggactcaat tcaggaagtg cattggatac tgtcaggcta
      121 gagtacagga gaggtgagcg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacaacaga ggcgaaggcg gctcactgga ctgttactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014909             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15081 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014909
VERSION     KHUT01014909.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15081"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           43 c           94 g           48 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggagtta ctgggcgtaa agggcgcgta ggcggttagg
       61 aaagagtgat gtgaaagccc tgggcttaac ctaggaagtg catcgcagac tatttaactg
      121 gagtataaga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ttaatactga cgctgaggcg cgaaagcgtg
      241 ggtagcgaac agg
//
LOCUS       KHUT01014910             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15082 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014910
VERSION     KHUT01014910.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15082"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           84 g           50 t
ORIGIN      
        1 tacgtaggtg gcaagcgata ctcggaatca ctaggcgtaa agcgcaggta ggcggtttgg
       61 taagtctttt gtgaaagctc ccggcttaac tgggagaggc caaaggaaac tactagacta
      121 gagtgtggca gaggaaactg gaattcccgg tgtagcggtg aaatgcgtag atatcgggag
      181 gaacacctat ggcgaaagca ggtttctggg ccatcactga cgctgatctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014911             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15083 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014911
VERSION     KHUT01014911.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15083"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           54 c          101 g           40 t
ORIGIN      
        1 tacgtagggg gcgagcgttg tccggaatta ttgggcgtaa agcgcgcgca ggcggtcctt
       61 taagtccggc gtttaagcgc ggggctcaac cccgaagagg cgccggaaac tgggggactt
      121 gagtgcggga gaggaaagcg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccagt ggcgaaggcg gctttctgga ccgtaactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014912             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15084 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014912
VERSION     KHUT01014912.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15084"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           92 g           47 t
ORIGIN      
        1 gacagagggt gctagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggttctg
       61 caagtcaggt gtgaaagccc ggggctcaac ctcggacgtg catctgaaac tgcttaacta
      121 gagtcccgga gagggtggtg gaattcctgg tgtagaggtg aaattcgtag atatcaggag
      181 gaacacccgt ggcgaaggcg gccacctgga cggagactga cgctgaggtg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014913             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15085 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014913
VERSION     KHUT01014913.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15085"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           89 g           51 t
ORIGIN      
        1 tacagaggtc ccaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggtggcgggg
       61 taagtcggat gtgaaatctc caggctcaac ttggaaatgg cattggaaac taccttgctg
      121 gaggatcgga ggggggactg gaatacttga tgtagcagtg aaatgcgtag atatcaagtg
      181 gaacaccagt ggcgaaggcg agtccctgga cgattcctga cgctgaggca cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014914             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15086 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014914
VERSION     KHUT01014914.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15086"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           58 c           98 g           42 t
ORIGIN      
        1 gacgaaccgt gcaaacgtta ttcggaatca ctgggcttaa agggcgcgta ggcggctcgc
       61 ccagtcgggg gtgaaatctt tcggctcaac cggaaaagtg ccttcgatac tggcgggctg
      121 gagggaggta ggggcatgcg gaacttccgg tggagcggtg aaatgcgttg atatcggaag
      181 gaacgccggc ggcgaaagcg gcgtgctgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014915             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15087 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014915
VERSION     KHUT01014915.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15087"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           48 c           90 g           51 t
ORIGIN      
        1 tacgtagggg gctagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggttcga
       61 ttagtctgct gtgaaagtca agagctcaac tcttgaaagc ccgtggatac tgtcggacta
      121 gagtacggaa gaggcgagtg gaattcctgg tgtagcggtg aaatgcgcag atatcaggag
      181 gaacaccaat tgcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014916             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15088 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014916
VERSION     KHUT01014916.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15088"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           85 g           52 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggggatg
       61 taagtcagat gtgaaatccc ggggctcaac cccggaactg catttgaaac tgcaactctt
      121 gaggatggaa ggagagaacg gaattccaca tgtagcggtg aaatgcgtag atatgtggag
      181 gaacaccggt ggcgaaggcg gttctctatt ttattcctga cgctgaggcg cgaaagcaag
      241 gggatcaaac agg
//
LOCUS       KHUT01014917             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15089 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014917
VERSION     KHUT01014917.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15089"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           60 a           54 c           97 g           42 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggctctg
       61 taagtccgtt ctgaaagtcc ggggctcaac cccgggaggc ggatggatac tgcagggctc
      121 gagtacggaa gaggcgagtg gaattcccgg tgtagcggtg aaatgcgcag atatcgggag
      181 gaacaccaat ggcgaaggca gctcgctggg acgttactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014918             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15090 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014918
VERSION     KHUT01014918.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15090"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           40 c           82 g           60 t
ORIGIN      
        1 tacggagggt gcaagcgttg tccggattta ctgggcgtaa agagtacgta ggcgctttgt
       61 taagtttttg tctaaatacc agggctcaac cttgggaaag gtgaaaatac tgacaaagtt
      121 gagggatgta gaggctacta gaactgtcgg tgtaggggtg aaatccgttg atatcgacag
      181 taattccaaa ggcgaaggcg agtagctgga catcttttga cgctgaggta cgaaagctag
      241 gggagcgaaa cgg
//
LOCUS       KHUT01014919             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15091 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014919
VERSION     KHUT01014919.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15091"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           46 c           82 g           55 t
ORIGIN      
        1 tacagagggt gcaagcgtta ttcgaaatta ttgggcgtaa agcgcgcgta ggtggtgagg
       61 caagtcttgt gtgaaatcct cgagctcaac tcgagaaagt catgagaaac taccttgctt
      121 gagtataaga ggggagagcg gaattcccag tgtagaggtg aaattcgtag atattgggag
      181 gaacaccggt ggcgaaagcg gctctctggc ttatcactga cactctggcg cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014920             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15092 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014920
VERSION     KHUT01014920.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15092"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           51 c           86 g           51 t
ORIGIN      
        1 tacgaaccgt acgaacgtta ttcggaatca ctgggcttaa agggtttgta ggcggccgtg
       61 taagtggggt gtgaaatccc tcggctcaac cgaggaattg cgccccaaac tacatggctt
      121 gagggagaca ggggtaagcg gaactgatgg tggagcggtg aaatgcgttg atatcatcag
      181 gaacaccggt ggcgaaagcg gcttactggg tctcttctga cgctgagaaa cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014921             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15093 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014921
VERSION     KHUT01014921.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15093"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           88 g           50 t
ORIGIN      
        1 gacagaggtg ccgagcgtta ggcggaatca ctgggcttaa agcgtgtgta ggcggacttc
       61 taagtgcctt gtgaaatccc atggcttaac catggaactg ctgggcatac tgggagtctt
      121 gagccactta ggggctagcg gaacaaatgg tggagcggtg aaatgcgtag atatcatttg
      181 gaacgccaat ggtgaaaaca gctagctggg agtgtgctga cgctgagaca cgaaagccag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014922             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15094 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014922
VERSION     KHUT01014922.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15094"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           61 c           95 g           39 t
ORIGIN      
        1 aacgtagggg gcgagcgttg cccggattca ctgggcgtaa agcgcgtgta ggcggtccgc
       61 caagtcggat gtgaaatcct acggctcaac cgtggaactg catccgatac tgacggactt
      121 gaggacggta gaggtcggtg gaattcccgg tgtaccggtg aaatgggcag atatcgggag
      181 gaacaccagt ggcgaaggcg gccgactggg ccgatcctga cgctgagacg cgaaagccag
      241 gggagcgaac cgg
//
LOCUS       KHUT01014923             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15095 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014923
VERSION     KHUT01014923.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15095"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           68 a           44 c           86 g           55 t
ORIGIN      
        1 tacgaagggt gcaagcgttg ctcggaatta ttgggcgtaa agcgagcgcg gatggtctcc
       61 taagtcagga gtgaaagccc tgggctcaac ccaggaagtg cttttgatac tgggagactt
      121 gaatgtggga gaggaagcta gaatacctag tgtagtggtg aaatacgtag atattaggtg
      181 gaacaccggt ggggaagccg ggtttctggc ccaacattga catttaggct cgaaagcgtg
      241 gggatcaaac agg
//
LOCUS       KHUT01014924             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15096 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014924
VERSION     KHUT01014924.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15096"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           83 g           58 t
ORIGIN      
        1 tacgaagggt gctagcgttg ttcggaatta ctgggcgtaa agcgtgcgta ggttgtctag
       61 ttagctagtg gtgaaagccc ggggcttaac cccggaattg ccattagaac tgctaggcta
      121 gagagtgtta gaggtaagtg gaattcctag tgtaggagtg aaatccgtag atattaggag
      181 gaacatcaat ggcgaaggca gcttactggg tcacatctga cactgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014925             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15097 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014925
VERSION     KHUT01014925.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15097"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           55 a           52 c          101 g           45 t
ORIGIN      
        1 tacggaaggg gcgagcgttg ttcggaatca ttgggcgtaa agggcgcgta ggtggtgctg
       61 tatgtctccc gtgaaagccc acggctcaac cgtggcgtgt cgggagaaac tgcaggactt
      121 gaggctggga ggggctggtg gaattccctg tgtagcggtg aaatgcgtag agatggggga
      181 gaacactcgt ggcgaaggcg gccagctaga ccagttctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014926             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15098 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014926
VERSION     KHUT01014926.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15098"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           52 c           95 g           44 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agcgcgtgta ggcggcttgg
       61 taagtccgtt ctgaaagtcc gaggctcaac ctcgggaggc ggatggatac tgtcaagctc
      121 gagtccggaa gaggcgagtg gaattccagg tgtagcggtg aaatgcgcag atatctggag
      181 gaacaccaat ggcgaaggca gctcgctggg acggtactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014927             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15099 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014927
VERSION     KHUT01014927.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15099"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           49 c           86 g           52 t
ORIGIN      
        1 tacgtagggt ccaagcgtta tccggaatca ctgggcgtaa agcgtgtgta ggcggaaatt
       61 tgcgtgtaat gcgaaatccg gtggctcaac catctggact gtattgcata cgggatttct
      121 ggaggtcggc agaggcaagt ggaattacta gtgtagcagt gaaatgcgta gagattagta
      181 ggaacaccaa tggcgaaggc agcttgctgg gccgcacctg acgctgagac acgaaagcgt
      241 ggggagcgaa cagg
//
LOCUS       KHUT01014928             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15100 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014928
VERSION     KHUT01014928.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15100"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           53 c           78 g           53 t
ORIGIN      
        1 tacatggagt ccaagcgtta tccggattta ctgggcgtaa agcgtccgca ggtttctcaa
       61 caagttcctg gtgaaatctc acggcccaac cgtgaaattg tcaggaaaac tattgagatc
      121 gagtatggca gaggcagaag gaattcccgg tgtaggggta aaatccgttg atatcgggaa
      181 gaacaccagt ggcgaaggcg ttctgctggg ccattactga cactcaggga cgaaagcgtg
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014929             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15101 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014929
VERSION     KHUT01014929.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15101"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           57 c           92 g           40 t
ORIGIN      
        1 tacgagaggt ccaagcgtta gtcggaatca ctgggcttaa agggtgcgta ggcggaccgg
       61 aaagcgtctt gtgaaatccc ctggctcaac cagggaaatg cagggcgaac tcccggtctc
      121 gaggaagcta gaggccggcg gaacgatcgg tggagcggtg gaatgcgtag atatcgatcg
      181 gaacgccaaa ggtgaagaca gccggctggg gctttcctga cgctgaggca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014930             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15102 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014930
VERSION     KHUT01014930.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15102"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           51 a           57 c          107 g           38 t
ORIGIN      
        1 tacagagggt gcgagcgttg tccggaatca ctgggcgtaa agggcgcgta ggtggcgcgg
       61 taagcgtgcg gtgaaagctc ggggctcaac cctgagtcgg ccgtgcgaac tgccgggctt
      121 gagcactgta gaggcgggtg gaattccggg tgtagcggtg gaatgcgtag agatccggaa
      181 gaacaccggt ggcgaaggcg gcccgctggg cagtggctga cactgaggcg cgacagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014931             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15103 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014931
VERSION     KHUT01014931.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15103"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           49 c           96 g           44 t
ORIGIN      
        1 tacggagggg gcaagcgttg ttcggaatca ctgggtgtaa agggtgcgta ggcggtgtgc
       61 caagtctgat gtgaaagccc tgggctcaac tcaggaatgg cattggaaac tggcagactc
      121 gagtaccgga gaggaaagcg gaattcctgg tgtagcggtg aaatgcgtag ataccaggag
      181 gaacaccggt ggcgaaagcg gctttctggc cgagtactga cgctgaggca cgaaagcgtg
      241 gggagcaaac ggg
//
LOCUS       KHUT01014932             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15104 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014932
VERSION     KHUT01014932.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15104"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           93 g           44 t
ORIGIN      
        1 tacatggggt gcaaacgttg ctcggaatta ttgggcgtaa agcgcgcgta ggcggtcgct
       61 taagtcggat gtgaaagccc ttggcttaac caaggaagtg catccgaaac tgagcagcta
      121 gagtacgaaa gagggtcgcg gaattcccgg tgtagaggtg aaattcgtag atatcgggag
      181 gaacaccggt ggcgaaggcg gcgacctggg tcgagactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014933             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15105 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014933
VERSION     KHUT01014933.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15105"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           54 a           61 c          101 g           38 t
ORIGIN      
        1 gacgaaccgt gcgaacgtta ttcggaatca ctgggcttaa agcgcgtgta ggcgggcgcg
       61 tgcgtcggat cctgaaatcc cccggctcaa ccggggaagc ggggccgata cggcgtgcct
      121 ggagggacgt agggggacct ggaacttccg gtggagcggt gaaatgcgtt gagatcggaa
      181 ggaacgcccg tggcgaaagc gaggtcctgg acgtctgctg acgctgagac gcgaaagcta
      241 ggggagcgaa cggg
//
LOCUS       KHUT01014934             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15106 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014934
VERSION     KHUT01014934.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15106"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           54 c           91 g           46 t
ORIGIN      
        1 gacgaaccaa gcaaacgttg ttcggattca ctgggcttaa agggcgcgta ggcggtcgga
       61 caagtccggg gtgaaatcct tcggctcaac cggagaatgg ccttggatac tgttcgacta
      121 gaagaggata ggggttgcgg gaactaccag tggagcggtg aaatgcgttg atattggtag
      181 gaacgccggt ggcgaaagcg cgcaactgga ccctccttga cgctgaggcg cgaaagctag
      241 ggtagcgaac ggg
//
LOCUS       KHUT01014935             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15107 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014935
VERSION     KHUT01014935.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15107"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           59 a           54 c           94 g           46 t
ORIGIN      
        1 aacgtaggtg gcaagcgtta tccggattca ctgggcgtaa agcgcacgca ggtggcaggg
       61 taggtagggc atgaaatcgc ctggcttaac taggcgaggt tgtcctatac cgctccgctt
      121 gaggctggga gagggaagtg gaattgccgg tgtagtggtg aaatgcgtag agatcggcag
      181 gaacaccagt ggcgaaggcg gcttcctggc ccagtcctga cactcaagtg cgaaagctag
      241 gggagcgaac ggg
//
LOCUS       KHUT01014936             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15108 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014936
VERSION     KHUT01014936.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15108"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           45 c           84 g           53 t
ORIGIN      
        1 tacagaggtc tcaagcgttg ttcggattta ctgggcgtaa agggtgcgta ggaggttttt
       61 taagtcgaat gtgaaatccc acagcctaac tgtggaactg cattcgatac tgaagagctg
      121 gagggtcgga gaggttgctg gaattctcgg tgtagcggtg aaatgcgtag agatcgagag
      181 gaacaccaat ggcgtaagca agcaactgga agaaccctga ctctgaggca cgaaagctag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014937             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15109 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014937
VERSION     KHUT01014937.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15109"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           61 a           53 c           98 g           41 t
ORIGIN      
        1 tacgtagggg gcaagcgttg tccggaatca ttgggcgtaa agggcgcgta ggcggtccga
       61 taagtccgct gtaaaagtcg agggctcaac cctcgaaggc cggtggatac tgtcggacta
      121 gagtccggaa gaggcgagtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccgat ggcgaaggca gctcgctggg acggtactga cgctgaggcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014938             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15110 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014938
VERSION     KHUT01014938.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15110"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           62 a           50 c           92 g           49 t
ORIGIN      
        1 tacgtaggga gcaagcgttg tccggaatta ttgggcgtaa agcgcgtgta ggcggtccga
       61 atagtctggt gtgaaatctg ggggctcaac cctcagctgt cactggatac tggcggactt
      121 gagtccggaa gaggagtgtg gaattcctgg tgtagcggtg gaatgcgcag atatcaggag
      181 gaacaccaat ggcgaaggca gcactctggt acgtgactga cgctgagacg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014939             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15111 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014939
VERSION     KHUT01014939.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15111"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           43 c           86 g           55 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattca ttgggtgtaa agggcaggta ggtggctata
       61 taagtccgat gtgaaatccc acggctcaac cgtggaactg cgttggatac tgtgtggctt
      121 gaatatcgga gaggtaaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaagcg ccttactggc cgattattga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014940             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15112 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014940
VERSION     KHUT01014940.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15112"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           77 a           35 c           81 g           60 t
ORIGIN      
        1 tacagagggt gctagcgtta atcggaatta ctgggcgtaa agggtgtgta ggttgctaaa
       61 taagttagat gtgaaaggcc tgggcttaac ctgggaattg cgtttaaaac tgttaagcta
      121 gagtattgca gagggtagta gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaataccagt ggcgaaggcg actatctggg caaatactga cactgagaca cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014941             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15113 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014941
VERSION     KHUT01014941.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15113"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           44 c           85 g           54 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggctgtcttg
       61 caagtctgac gtgaaatccc acggctcaac cgtggaaatg cgctggaaac tgcttgactt
      121 gagtatcaga gaggtaaagg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccaga ggcgaaggcg ctttactggc tgattactga cgctgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014942             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15114 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014942
VERSION     KHUT01014942.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15114"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           58 a           55 c           97 g           43 t
ORIGIN      
        1 gacgaaccgt gcgaacgttg ttcggaatca ctgggcttaa agggcgcgta ggcgggcaag
       61 caagtccggg gtgaaagccc ccagcttaac tggggaagtg ccttggaaac tgcttgtctc
      121 gagggaggta ggggtctgtg gaacttcggg tggagcggtg aaatgcgttg atatccgaag
      181 gaacgccggt ggcgaaagcg acggactgga cctcttctga cgctgaggcg cgaaagccag
      241 gggagcaaac ggg
//
LOCUS       KHUT01014943             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15115 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014943
VERSION     KHUT01014943.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15115"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           57 a           52 c           97 g           47 t
ORIGIN      
        1 cacgtagggg gcgagcgttg tccggattta ttgggcgtaa agagcgcgta ggcggcttcc
       61 taagtcggac gtgaaattcc aaggctcaac cttggaacgc cgttcgatac tgggaggcta
      121 gaggcaggta ggggaaggtg gaatacctgg tgtagcggtg aaatgcgtag atatcaggtg
      181 gaacaccggt ggcgaaggcg gccttctggg cctgtcctga cgctgaggcg cgaaagctgg
      241 gggagcaaac agg
//
LOCUS       KHUT01014944             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15116 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014944
VERSION     KHUT01014944.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15116"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           50 c           88 g           51 t
ORIGIN      
        1 tacgagtgcc ccaagcgtta tccggaatca ttgggcgtaa agggtgtgta ggtggtcgtg
       61 ttagtctgca gtgaaatctc ccggcttaac tgggaatccg ctgtggaaac ggcacgacta
      121 gaggatgcga ggggtctgtg gaactcatgg agtaggggtg aaatccgttg atatcatggg
      181 gaacaccgaa agcgaaggca gcagactggc gcattcctga cactgagaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014945             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15117 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014945
VERSION     KHUT01014945.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15117"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           39 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgtta atcggaatta ctgggcgtaa agggcatgta ggtggaataa
       61 taagtttgat gtgaaaggcc tgggcttaac ctgggaatgg cgttgaagac tgttaatcta
      121 gagtttgaca gagggtcgtg gaatttccgg tgtagcggtg aaatgcgtag agatcggaaa
      181 gaacaccagc ggcgaaggcg gcgacctggg tcaaaactga cactgaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014946             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15118 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014946
VERSION     KHUT01014946.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15118"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           64 a           47 c           99 g           43 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggcgcgta ggcggtgatg
       61 cgagtgtgat gtgaaagccc cgggcttaac ctgggaagtg catcgcaaac ggcatgactg
      121 gagtatagga gagggtggcg gaatttccgg tgtagcggtg aaatgcgtag agatcggaag
      181 gaacgtcgat ggcgaaggca gccacctggc ctaatactga cgctgaggcg cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014947             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15119 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014947
VERSION     KHUT01014947.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15119"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           71 a           48 c           82 g           52 t
ORIGIN      
        1 tacgaagggt gcgaacgtta ttcggattta ttgggcgtac agggtgcgca ggcggcaagt
       61 taagtcaact gttaaatctc ccagcctaac tgggaatcag cggtagatac tggcatgcta
      121 gagggtggaa gagagaagtg gaattcttgg agtagcggta aaatgcgtag atctcaagag
      181 gaacaccgat ggcgaaggca gcttcttggt ccacatctga cgctcatgca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014948             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15120 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014948
VERSION     KHUT01014948.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15120"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           70 a           47 c           82 g           54 t
ORIGIN      
        1 tacggagggt gcaagcgtta tccggattca ctgggtttaa agggtgcgta ggcggcccgg
       61 taagtcagtg gtgaaagctc ccagcttaac tgggaaactg ccattgaaac tgccaggctt
      121 gaatacagtt gaagtaggcg gaatacaaca tgtagcggtg aaatgcttag atatgttgta
      181 gaacaccgat tgcgaaggca gcttactaag ctgtgattga cgctgaggca cgaaagcgtg
      241 gggagcgaac agg
//
LOCUS       KHUT01014949             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15121 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014949
VERSION     KHUT01014949.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15121"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           76 a           39 c           82 g           56 t
ORIGIN      
        1 tacagagggt gcgagcgtta atcggaatta ctgggcgtaa agggtgtgta ggtggtcata
       61 taagttagat gtgaaatccc cgggctcaac ctgggaactg cgtttaagac tgtatgacta
      121 gagttttgta gaggatagtg gaatttccag tgtagcggtg aaatgcgtag atattggaag
      181 gaacaccagt ggcgaaggcg actatctgga caaaaactga cactgagaca cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014950             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15122 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014950
VERSION     KHUT01014950.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15122"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           78 a           41 c           80 g           54 t
ORIGIN      
        1 gacataggtg gcgaacgtta tccggcttta ttgggcgtaa agagtgcgta ggtggcgaaa
       61 taagttagcg gtgggaaatc ggggctcaac ctcgtggaag ccggtaatac tatttcgcta
      121 gaggacagga gaagttgacg gaactctatg tgtaggggta aaatccaaag atatatagaa
      181 gaacatcaaa ggcgaaggca gttaactatt ctgttcctga cattgaggca cgaaagcgtg
      241 gggagcaaaa cgg
//
LOCUS       KHUT01014951             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15123 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014951
VERSION     KHUT01014951.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15123"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           93 g           45 t
ORIGIN      
        1 tacgaggggt gcaagcgttg ctcggaatta ttgggcgtaa agggtaggta ggtggtctca
       61 tttgtctggg gtgaaagccc ggagcttaac tccggaagtg ccccagaaac ggtgagactc
      121 gagtactgga gagggtcgtg gaattcccgg tgtagcggtg aaatgcgtag agatcgggag
      181 gaacaccaga ggcgaaggcg gcgacctgga cagtaactga cactcaacta cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014952             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15124 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014952
VERSION     KHUT01014952.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15124"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           43 c           88 g           56 t
ORIGIN      
        1 tacgtagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcgtgta ggtggtttac
       61 caagtcagtt gtgaaagccc tcggctcaac cggggaagtg cagttgaaac tggtagacta
      121 gagaacggta gaggaaggtg gaattccaag tgtagaggtg aaattcgtag atatttggag
      181 gaatacctgt ggcgaaggcg gccttctggg ccgtttctga cactgagacg cgaaagcgtg
      241 ggtagcaaac agg
//
LOCUS       KHUT01014953             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15125 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014953
VERSION     KHUT01014953.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15125"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           73 a           41 c           83 g           56 t
ORIGIN      
        1 tacggagggt gcaagcatta atcggattta ttgggcgtaa agggcgcgta ggcggttttg
       61 taagtcagat gtgaaatacc aaagctcaac tttggtgctg catttgaaac tgtaagacta
      121 gagggatggc ggagaaaacg gaattccacg tgtagcggtg aaatgcgtag atatgtggaa
      181 gaacacctgt ggcgaaagcg gttttctagc tattacctga cgctgaggcg cgagagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014954             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15126 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014954
VERSION     KHUT01014954.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15126"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           88 g           52 t
ORIGIN      
        1 gacagaggtg gcaagcgttg ttcggattta ttgggtgtaa agggcaggta ggtggctttg
       61 caagtctgac gtgaaatccc agggctcaac cctggaactg cgttggaaac tgcatagctt
      121 gagtaccgga gaggtaaggg gaattctcgg tgtaagggtg aaatctgtag atatcgagag
      181 gaacaccagt ggcgaaggcg ccttactggc cggttactga cactgagctg cgaaagcaag
      241 gggagcaaac agg
//
LOCUS       KHUT01014955             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15127 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014955
VERSION     KHUT01014955.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15127"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           41 c           87 g           53 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatta ttgggcgtaa agggagtgta ggcggttagt
       61 taagtctcat gtgaaagctc cgagctcagc ttggaaagtg catgagaaac tggttaactt
      121 gagtgtcaaa gaggagagcg gaattcccgg tgtagaggtg aaattcgtag agatcgggag
      181 gaacaccagt ggcgaaagcg gctctctggt tgacaactga cgctgagact cgaaagcatg
      241 gggagcaaac agg
//
LOCUS       KHUT01014956             254 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15128 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014956
VERSION     KHUT01014956.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15128"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>254
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           50 c           86 g           52 t
ORIGIN      
        1 gacgtagggt ccaagcgttg tccggattta ctgggcgtaa agagcgcgta ggcggcttgt
       61 taagtgtgaa gtgaaatctc cagggctcaa cccggaaact gctttacata ctggctagct
      121 agaggaatgc agaggtcagt ggaattcctg gtgtagcggt gaaatgcgta gatatcagga
      181 ggaacaccca tggcgaaggc agctgactgg gcattatctg acgctgaggc gcgaaagcgt
      241 gggtagcaaa cagg
//
LOCUS       KHUT01014957             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15129 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014957
VERSION     KHUT01014957.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15129"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           69 a           47 c           92 g           45 t
ORIGIN      
        1 tacggaggat gcaagcgtta tccggaatga ttgggcgtaa agggttcgca ggtggtagtg
       61 aaagtctgct gttaaaggtc ggggctcaac cccgaacagg cagtggaaac tacacgacta
      121 gagtacggta ggggcagagg gaattcctgg tgtagcggtg aaatgcgtag agctcaggaa
      181 gaacaccggt ggcgaaagcg ctctgctaga cctgtactga cactgaggga cgaaagctag
      241 gggagcgaat ggg
//
LOCUS       KHUT01014958             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15130 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014958
VERSION     KHUT01014958.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15130"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           53 a           55 c           97 g           48 t
ORIGIN      
        1 tacgtaggtg gcaagcgttg tccggattta ctgggcgtaa agcgcgcgca ggcggctgat
       61 taagtccgat gtgaaatctc ccggctcaac cgggagcggt cattggatac tggtcggctt
      121 gagggaggca gaggagagtg gaattcccgg tgtagtggtg gaatgcgtag atatcgggag
      181 gaacaccagc ggcgaaggcg gctctctggg cctttcctga cgctgaggcg cgaaagcgtg
      241 gggagcaaac cgg
//
LOCUS       KHUT01014959             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15131 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014959
VERSION     KHUT01014959.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15131"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           65 a           47 c           90 g           51 t
ORIGIN      
        1 tacagagggt gcaagcgttg ttcggaatca ttgggcgtaa agcgcacgta ggcggcttgc
       61 taagtttggt gtgaaagccc agagctcaac tctggaagtg cactgaatac tggcaagcta
      121 gaggacgaga gaggatggtg gaattcctgg tgtaggggtg aaatccgtag atatcaggag
      181 gaacatcagt ggcgaaggcg gccatctggc tcgtttctga cgctcaggtg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014960             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15132 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014960
VERSION     KHUT01014960.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15132"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           72 a           44 c           80 g           57 t
ORIGIN      
        1 tacagaggcc tcaagcgtta tccggaatta ctgggcgtaa agggtgtgta ggtggtttag
       61 ttagtctttc gttaaattct tcggcttaac cggggacatg cgggagaaac ggctagacta
      121 gaggatgtga gaggtacatg gaactcatgg agtaggggtg aaatccgttg atatcatgag
      181 gaacaccaaa agcgaaggca gtgtactggc acattcctga cactcaaaca cgaaagcgtg
      241 ggtagcgaat ggg
//
LOCUS       KHUT01014961             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15133 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014961
VERSION     KHUT01014961.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15133"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           74 a           43 c           83 g           53 t
ORIGIN      
        1 tacagagagt gcaagcgtta atcggaatta ctgggcgtaa agcgcgcgta ggtggatatt
       61 taagtcggat gtgaaagccc tgggcttaac ctaggaattg cattcgatac tggatatcta
      121 gagtatggta gagggaagtg gaatttccgg tgtagcggtg aaatgcgtag atatcggaaa
      181 gaacaccagt ggcgaaggcg gcttcctgga ccaatactga cactgaagcg cgaaagcgtg
      241 gggagcaaac agg
//
LOCUS       KHUT01014962             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15134 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014962
VERSION     KHUT01014962.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15134"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           66 a           49 c           84 g           54 t
ORIGIN      
        1 tacgtagggc ccgagcgtta tccggtatta ttgggcgtaa agcatacgta ggcggtttgg
       61 taagacaatg gttaaagctc cgagctcaac tcggaaatcg ccgttgttac tgccaaactt
      121 gtcggtagca ggggcaagcg gaattctcgg tgtaggagtg aaatccgttg agatcgagaa
      181 gaacaccaat ggcgaaggca gcttgctagg ctacttgaga cgctgaggta tgaaagcgtg
      241 gggagcgaac ggg
//
LOCUS       KHUT01014963             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15135 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014963
VERSION     KHUT01014963.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted Locus Study; ENV.
SOURCE      soil metagenome
  ORGANISM  soil metagenome
            unclassified sequences; metagenomes; ecological metagenomes.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Khatri-Chhetri,U.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2023) Agricultural, Food & Nutritional Science,
            University of Alberta, 116 St & 85 Ave, Edmonton, Alberta T6G 2R3,
            Canada
COMMENT     Sequences were screened for chimeras by the submitter using
            Usearch.
            
            ##Assembly-Data-START##
            Assembly Method       :: Paired End reAd mergeR v. 32-bit version
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="soil metagenome"
                     /mol_type="genomic DNA"
                     /submitter_seqid="OTU15135"
                     /isolation_source="Grassland soil"
                     /db_xref="taxon:410658"
                     /environmental_sample
                     /country="Canada: Prairie provinces of Alberta,
                     Saskatchewan, and Manitoba"
                     /lat_lon="50.87376425 N 111.87538659 W"
                     /collection_date="2017"
                     /note="metagenomic"
     rRNA            <1..>253
                     /product="16S ribosomal RNA"
BASE COUNT           67 a           46 c           87 g           53 t
ORIGIN      
        1 tacgtaggtg gcaagcgtta ctcggaatta ctaggcgtaa agcgcaggta gctggttagg
       61 taagtccgca atgaaatctc ccggcttaac tgggaggggt ttgtggatac tgcttgactc
      121 gagtgtggga gaggtcactg gaattccagg tgtagcggtg aaatgcgtag atatctggag
      181 gaacaccaat ggcgaaagca ggtgactggc ccaatactga cggtgagctg cgaaagctag
      241 gggagcaaac agg
//
LOCUS       KHUT01014964             253 bp    DNA     linear   ENV 03-MAR-2023
DEFINITION  TLS: soil metagenome OTU15136 16S ribosomal RNA, sequence.
ACCESSION   KHUT01014964
VERSION     KHUT01014964.1
DBLINK      BioProject: PRJNA923480
            BioSample: SAMN32723588
KEYWORDS    TLS; Targeted